LOCUS       DAOQAD010000001        50942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_527_length_50942_cov_6.910134, whole genome shotgun sequence.
ACCESSION   DAOQAD010000001 DAOQAD010000000
VERSION     DAOQAD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 50942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50942
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_527_length_50942_cov_6.910134"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2061
                     /locus_tag="PKL60_00005"
     CDS             <1..2061
                     /locus_tag="PKL60_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNW94549.1"
                     /translation="EQLTRLIQTALNERGVQAPEITIEFNPETAPTEMLFNQGMMIEK
                     MPEEKRAPYEARLHEIKVVLIRSLISDQLPYINIAKDWFTVSDLAEIRQHKLGRGRIG
                     GKAAGMLLAARILKEKAGLRLRESLQTPTSFYIGSDVFYNFLSINNLHHWNDQKYKDE
                     EQMRSDYPRIVEDFIRGDFPPTAVQHQETILSMAGKRPLIVRSSSLLEDNFGTAFAGK
                     YESIFLPNQADPEENLRALQQAIARIYASTLNPPALLYRKSRGLLDYDERMALLIQVV
                     EGQQIGPYYFPQLAGVGYSQNQFRWSPQIRPQDGFVRLVWGLGTRAVDRVGNDYPRLI
                     ALSHPTLTPSSSAQSIRRYSQQFIDLIDLQQNRFTTLPIRAVLSQKVPQMRFLAQLDS
                     EGYFQTLRSNLVEGNPENLVLTFDELLRRTDFPQLMRELLATLESEYHTPVDIEFTAR
                     VAENDGSPAVQITLIQCRPLGTIKEGKYELPQRVADNDKIFSGIGVVTGGMIEGVRYA
                     LYVKPDAYFAMPTQEERNQLERAIGKINAALHGENYICVGPGRWGTSNPDLGVHVDYA
                     DIFNARAIVELTGGAVGIPDEPSLGTHFFQDLLEGQIYPLAVNLNRDTFNTRFFESTP
                     NHLLEWLPDAEALQHCLRVIRTADYSPESRLDIVIDDLNGSALAFLSGETVKGGEKRQ
                     LIVP"
     gene            2665..2737
                     /locus_tag="PKL60_00010"
     tRNA            2665..2737
                     /locus_tag="PKL60_00010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2698..2700,aa:Arg,seq:gcg)
     gene            2812..4044
                     /locus_tag="PKL60_00015"
     CDS             2812..4044
                     /locus_tag="PKL60_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94550.1"
                     /translation="MKKHLFPYLFLLLAALALAACASKAAPEAVLPESAYPAGANART
                     VYPMSTPTSTSAPATAESPGAVSPAPIPLFSIRIFPDGRGWAANWNQTALYHTADSGL
                     NWTEVSPADAISGASGGFYSFFLDGSTAWLSLPLFESSSLLRTTDSGTTWSTTPVDFS
                     AGNPWFLDSQNGFLLSRLDAAAGSEFVGIYQTADGGLSWVKRFSHEPGMADPSLPAGG
                     QKSGFTFPNLINGWVAGSEPINDFIYLYRSTDSGVNWLLQDVPLPDDAIGAMFESLPP
                     VFFNASEAVLPVRIYLPDGDAHTGFYRSTDGGETWSFLIGALPPANRFVFADAQNWWL
                     LGESTFLRTGDGGLTWEDLSAALPVGFTPLNLDFADANHGWLLVSPVGDLELESNFLY
                     GTSSGGSAWELLPARLTP"
     gene            4171..4998
                     /locus_tag="PKL60_00020"
     CDS             4171..4998
                     /locus_tag="PKL60_00020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNW94551.1"
                     /translation="MKKCLFSILNLAALAGLALTAPAPVLAAAQAQALPPASQSADPG
                     RTQSFIQLVNNWTAPDQPADSAQDLPHANPDLVVYLTFDDGPDPDWTPQILALLQEYH
                     ATAVFYEIGRNARTYPQISLEVAEAGQMIGLHGYNHIELSKLGYNDFYLEVIDTRDAI
                     QDALSSRPDLEGQLTPCLRPPYGAVNDAVYSYAYNMNYAISMWHLDTRDWTGISGEEI
                     LATVEKELVPYRVVLMHDGGEDRSETVRGLELVLHELTLRGYHFEPYCTATGQEYNH"
     gene            5254..5835
                     /locus_tag="PKL60_00025"
     CDS             5254..5835
                     /locus_tag="PKL60_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNW94552.1"
                     /translation="MAQYKGRYCPLCGAQLERAQKALEPGAPEQDYSINKGALEGLLD
                     NIRSALNIGAVFRAADGAGLRKLWLTGITPRPTQQAQIAKTALGAEQAVPWESAPNAL
                     TLAADLKARGYTLLALENAPNARPLFSFRSDAPKHSPLLLIVGSEQAGVDPGLLALAD
                     ETLSLPMHGSKGSLNVAVAFGIAAYWLRYAPLT"
     gene            complement(6021..7274)
                     /locus_tag="PKL60_00030"
     CDS             complement(6021..7274)
                     /locus_tag="PKL60_00030"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNW94553.1"
                     /translation="MPKTSEILGVILGGGQGKRLYPLTLERSKPAVPIAGKYRLIDIP
                     ISNCINSDIFRIAVLTQFNSVSLHRHISQTYLFDSFHPGWVQIWAAEQTMEHTDWYQG
                     TADAVRKQLMEIISTKCPYVLILAGDHLYRMDYAEMAQFHWDNNADITVAVQPVPKSE
                     ASRFGLLKRAEDGRITDFAEKPKDPIKLASMVSRDDPESPYLGSMGIYMFNTELLVKV
                     LRKGGERLDDFGGDIIPYLIGQGASVYGYNYEGYWRDIGTIRSFYETNLELTDDQPGF
                     DFYDPDKPIFTHPRFLPCSRMVGCSFKQVLIAEGCNIENATIEHSVIGVRSQIGAGSV
                     VKDTIMMGGDYYGMLKNGAPLGIGKNCTIQGAIIDKNSSIGDNVVIKPFPVDMEVDHA
                     LYTVRDGIVVIPKHTTIPAGTVIAP"
     gene            complement(7359..9095)
                     /gene="recN"
                     /locus_tag="PKL60_00035"
     CDS             complement(7359..9095)
                     /gene="recN"
                     /locus_tag="PKL60_00035"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNW94554.1"
                     /translation="MLAELRIQNFAIIQELTLTFSNGLVVLTGETGAGKSIILDALNA
                     VLGGRVDVNDIRRDSDKAVIEASFALDAPTAAGVRALLEPEGLWEEDGTLSLSREIRA
                     GGRSVARVNGRSVSLSLQSELGSLLADIHGQSEHLSLLKVRRHLELLDRFAHHEDALS
                     EYTRLYRQWLAYRSELEELLNLQQYAKDRTDMLKYQIQEIESARLKADEENALRQERT
                     LLANAETLHSLSRQALSALDEGADALPVTDLLGEAQHALNELARIDPQMSAMSERAVQ
                     ALNTVSEIAYELRGYLENIEFNPARLDQIEERLNTLNFLKRKYGGSVESVKAYLQKAK
                     DELSKVETVEDQISEVEQKISRIKQELAVLAARLSAERRSAAQKLENGVETQLQSLQM
                     KNARFQVSFSALMDPEGLEVDGQRLAFDAAGVDRVEFLIETNPGEGLKPLVKTASGGE
                     TSRLMLALKHVLAEADQIPTLVFDEIDQGIGGRVGVIVGQMLWQLGRKHQVMCVTHLP
                     QLAAYGDQHLRVSKHETDGRTLTQVSELEAGAREQELAGMIGSVSAGTLQSARDLLHA
                     VEGFTGKINTVR"
     gene            complement(9104..9631)
                     /locus_tag="PKL60_00040"
     CDS             complement(9104..9631)
                     /locus_tag="PKL60_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94555.1"
                     /translation="MTETNLPTPNAAQEEIVLRCNRCNKPITPETAVLTPTGYRCKDC
                     VRSQQKIYDTNKGLDVPVGLLISALIAFGGSWLAGRIGFLILFVAPGIGTLIANVVRW
                     AVRKRRSQALNKALLWGSILGCLPLLILRLLPIFGANGAQAALVGLLPLVWQAVYALL
                     LVSSAYYQSTGIKLN"
     gene            complement(9624..10448)
                     /locus_tag="PKL60_00045"
     CDS             complement(9624..10448)
                     /locus_tag="PKL60_00045"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNW94556.1"
                     /translation="MPKALQIAGLLSERLSRAGAAVSTFVAAPAAIPSGPDLSSADLV
                     IVLGGDGTLLRAGHLCAPLGIPLLGVQAGKLGFLSELTESTWEEALLRLLSGEFHLEQ
                     RMMLLAQLIQDERVCGEWDVINEIVVSRGRIVRPIEVRVDLNEGYLTSYIADGLIVAT
                     ATGSTAYALAVGGPILPPELRNMLLLPIAPHLSLDRAVVLAEGASVVLRAHCDHEAVI
                     SVDGIDPIRLNDNDAVRVTANQHSLKMVRFGEPNYFYRRLATVLYDNPALKQNHHD"
     assembly_gap    10960..11059
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11060..11317
                     /locus_tag="PKL60_00050"
     CDS             <11060..11317
                     /locus_tag="PKL60_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94557.1"
                     /translation="AVTPPDLQPQAVGLRCRSTRPTTLLPFHLTYDHVLSGCAAVPTN
                     LTLLPFHPTYDFAAAPTDLQPRAVGLRCRSTRPTTFLPPHS"
     gene            11463..12971
                     /locus_tag="PKL60_00055"
     CDS             11463..12971
                     /locus_tag="PKL60_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNW94558.1"
                     /translation="MHKRLNILLACVLCLLPGLARPLPAFALPPAPQSTLPDFPCYRD
                     LATLYADAAALASAYPALATWTDIGDSWEKTQPGGAPGYDLFALRLTNSALPGEKPAL
                     ILVSGTHARDLAPVELNLRFAEYLLAGYGSDQDLTWLLDSAEVHIILSANPDGRAVVE
                     AQIASGEADEFGDTARKKNLNNTACPDPILTGVDLDRNFSFQWQLGPDGCMSDYPGAA
                     AASEPETNALIAYLENTLEDYRAGGPDEPADPNARGLLVHLQSYGNKVFYPYSYRSEP
                     APEDAALQMLANKFAYGTPASPAKYSGTYASFLPGSLADYVYGERGVPALAYMLFANM
                     DGRYFTRCSVFGNSLSANLQALTRALKTVWAPYIFPAGPEMTPLLITVHHSESETWWE
                     LLTSADTNMLKDPDPNAPPVASAVYSFDLPPWEPGAVLHALPTQDGAFDSPQEQLHSR
                     LDFSGLEPGWHRVFAQAFLADGTPGLAAAQSVLVPEPPPTTPYSSYLPILLR"
     gene            complement(12984..14042)
                     /locus_tag="PKL60_00060"
     CDS             complement(12984..14042)
                     /locus_tag="PKL60_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNW94559.1"
                     /translation="MNPSLPALNYAFLEDFLVRLLNTPSPTGYAARAVELTRQTLAEF
                     PQLSLRVTKKGALLAVLPGADAQPEPSRALTGHVDTLGGMVRVIKPSGRLELTSIGGF
                     AWNAVEGEGVWVHTQSGVLVRGSLLPNHASVHVYSDVKDQKRSPDTMEVRLDARTSSA
                     EETRALGIQVGDFVSFDPRVELTNGFVRSRHLDDKACAACMAAAIQALCAAGLEPARS
                     TCFLFSNFEEVGHGGAADIPASVEELVAVDMAAMGEGQASDEFHATLCVKDSGGPYHN
                     GLSNRLRALAENYVIPYKVDIYPNYGSDATAYWHAGGAAAAALIGPGVDASHHYERTH
                     REALEATTNWILAYLLSE"
     gene            complement(14039..14494)
                     /locus_tag="PKL60_00065"
     CDS             complement(14039..14494)
                     /locus_tag="PKL60_00065"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNW94560.1"
                     /translation="MTSLTPAYPKQFTFYHPIAVRYADLDTHRHVNNISVLEYVETAR
                     TAYYQAAGIWDGVTVENMGMVVVSVKCDYLESICFDQSVRVGLTLDSIGTKSLRFRFQ
                     VEDGEGTRAFARGDVVMVSYDHFEGHSRPVSAEWRHKLAAFENREDFLA"
     gene            15146..15880
                     /locus_tag="PKL60_00070"
                     /pseudo
     CDS             15146..15880
                     /locus_tag="PKL60_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            15881..16255
                     /locus_tag="PKL60_00075"
     CDS             15881..16255
                     /locus_tag="PKL60_00075"
                     /inference="COORDINATES: protein motif:HMM:NF037895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94561.1"
                     /translation="MRSACKRLSRFTQLEFNAPIFITPNPRSLERVPFGALVEQGFIE
                     PGQALFFGLEDDLKATVQADGSLVCRGERGSIHALARSLRKGPANGWELWYYWDTETQ
                     CRQPINKLRAKYRESVKKHYSG"
     gene            16277..18052
                     /locus_tag="PKL60_00080"
     CDS             16277..18052
                     /locus_tag="PKL60_00080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNW94562.1"
                     /translation="MMEHKETYTQSRWSLDDLFTGFEDANIEATYEKLDAMVAEFEKC
                     RGELKPDISAERFMEIVASSEAGEKLGYRLYGFAELSFAANTQDQKAQIAVARIQQFV
                     AEMSNRTLFFNLWWKALDDANAQRLLAASGDYRYWLEQIRNFKPYTLSEAEEKVINIK
                     NVTGSNALNILYDSLTNRYVFHLEVDGEQKELTRGELMTYVRSGDPELRAKAYQELYR
                     VYGEDAPILGQIYQNLVRDWRNENVSLRGFGSPISVRNLINDIPDEIIDTLIEVTRKN
                     LGVFHRYFKLKAKKLGVEKLRRYDIYAPVTAADKRFSYEEAVALTFEAFERFDQAFAS
                     KARRVFDEKHVDSEVRKGKMGGAFCSTILPELTPWVLLNYQGKSEDVSTMAHELGHAV
                     HSMMAAEHTLYTQHACLPLAETASTFGEMTLTDLMLERETDASVKEDILFGQLDDAFA
                     TILRQIYFAIFERDAHEKIIRDASVDELSELYLENLKEEFGDAVDVSEEFKYEWVSIP
                     HIYGTPFYVYAYAFGQLLVFALYKRYQEEGESFKPKYMRILSAGGSVAPVQLLAEAGL
                     DVTKAEFWQGGYDIIDGMVSRLENL"
     gene            complement(18119..19015)
                     /gene="metF"
                     /locus_tag="PKL60_00085"
     CDS             complement(18119..19015)
                     /gene="metF"
                     /locus_tag="PKL60_00085"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNW94563.1"
                     /translation="MNITKLFDHKKTVFSLEIFPPKKDTSYNTIYNTLLRLRGIPADF
                     ISVTYGAGGSQAQREKTIEIASLILSTYHVEPVAHLTCVNLDRPEALETLEKLKANQV
                     QNIMVLRGDISPDVPPKEDFRHASDLAAFIKQYDPSFNLLGACYPECHYQCETLEKDI
                     ENLKIKIDNGVGHLITQLFFDNEKFYIFRDMAERAGIHVPIEAGIMPITNIRQIERTV
                     ALSGASVPARLSRIFSRYGDNPKALFDAGIYYATEQIMDLIDHDIRGIHLYTMNNVET
                     ATRISDAIQNVLEAENTPKNAA"
     gene            19169..19378
                     /locus_tag="PKL60_00090"
     CDS             19169..19378
                     /locus_tag="PKL60_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94564.1"
                     /translation="MEIDVETERVPRSREVHVHESSAFYGLGFIGAAIYFISTATTFW
                     MGVLGFLKAFVWPAFLVYEALKVLY"
     assembly_gap    19894..19993
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20348..20986
                     /locus_tag="PKL60_00095"
     CDS             20348..20986
                     /locus_tag="PKL60_00095"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNW94565.1"
                     /translation="MFIAIEGVIGVGKTTLARFVHSAFSADLLLEVFEENPFLSDFYS
                     DRSRYAFQTQIFFLLSRYQQQHRVIPDTLAAGRMIISDYTFEKDALFAGINLAGDELA
                     MYHRVHAALAEKIPQPDLVVYLRASTETLMQRIAHRDRPYERGMDRAYIDELNQAYEA
                     FFAHPRWEGRLLPIETDALNIFARPDDLNYVINRIRQTLRIAPYQESLPLEG"
     gene            21000..22007
                     /locus_tag="PKL60_00100"
     CDS             21000..22007
                     /locus_tag="PKL60_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94566.1"
                     /translation="MHITRDLLVKFAREYVAKKTRQSNDIVAVYLTGSVLTDDPLIGG
                     STDIDLVVVHKENPLFEREVQRISYEISLDVQHHHQSFYTFHRRLRLNPWLGFALCDH
                     RSILYDTEHWLEFIQAGVSAGFNSPETVYARGQAAADKARQLWFELEDPQELPFALWT
                     DLYYKAVGSAANAIAIINGPALTTRRMLLDFPARAEALGKLELTGELLQIISRAPINT
                     EILNTWRPVWENALSAASKLPDCPPNLLPARKAYFVNSCAAMLESGSAHAALWPMVET
                     WRQANLLLSEDPAQQEAWQKFLEALEITPFDKDEQIERLDLFLDNAEAALAEMKAEYG
                     L"
     gene            complement(22435..23067)
                     /locus_tag="PKL60_00105"
     CDS             complement(22435..23067)
                     /locus_tag="PKL60_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNW94567.1"
                     /translation="MDKFIVVAGNIGAGKSTLVQILSERLGFKPFFEPVNDNPYLEDF
                     YRNMEAWSFHSQLYFLTRRLRIHKQLLDAEGSVVQDRSVYEDAEVFARNLYLQEDMST
                     RDYGVYQDLYRILIDLLPPPNLVVYLRTSVDTLLQRINTRGRGFEAGISRSYLERLNV
                     LYEEWIASFAQCPVLIIPSDNLNLVSSSAHISQVVQLVQERLMGKTEVNL"
     gene            complement(23152..24336)
                     /locus_tag="PKL60_00110"
     CDS             complement(23152..24336)
                     /locus_tag="PKL60_00110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW94568.1"
                     /translation="MPNPISPRTKAEIFLFTLTRTVINSGYRMVYPLLPLFAAGMGVQ
                     LADLSIAFSIRSVLGIFNPFLASMADARGRKNGLMLGVILFFVGCGITALGQNFLSFI
                     IGLSVFGVGNGIFIPSMQAYLGEKVPYERRGTVLSITELSWSLGFIVGVPVLGALLNT
                     GGWVAPFSALTIAGVLLTAALWLLVPAQRAPANQGTSVLANLGKALRYFPVLAAALVG
                     IFSTGANEMINLIFSVWIKDTFGLDFAALAVASVIIGTSELGSELLSAAVLDKIGKHR
                     AMRLALIINGLVALALPLTRTSFSLALVGLALFYVSFEFALISLMTTVSEVLPNSRAA
                     VLAILMAALSIGRLLGPLFSPGLYQNSFWLVCAAAAGLNAGAVLLVPGIRVSPQGAAV
                     ES"
     gene            complement(24369..24791)
                     /locus_tag="PKL60_00115"
     CDS             complement(24369..24791)
                     /locus_tag="PKL60_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNW94569.1"
                     /translation="MAMQITNQADYALRSMIYIARNGQNRLTPSNVIAEEMQISRIFL
                     SRINSELVGAGLIKTRRGARGGIMLAKSPAEISIYDIVTTIDGKINLIHCTEDPETCP
                     LADACPYLKFWKETEDQIIAQLKGTTLQDMLDQPATPA"
     gene            complement(24893..27223)
                     /locus_tag="PKL60_00120"
     CDS             complement(24893..27223)
                     /locus_tag="PKL60_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNW94570.1"
                     /translation="MNTAAPAPPPTPDPTQVHIPAVMPFFWLACAGLCGAVGASWLRL
                     PWQWWAGMLGLCLLGALGHAKRRFRWQPRKQLPLSLLAAGFCLAALLYQLSLPKDTAA
                     FIGYYVGKGEVEVLGVVSETPEAWENGQELVVEVQAVNALSADVEAVRPEKVRGKILL
                     RTMPGSAYAYGDLLSIRGELTEAGESGTFSYKAYLFHQGISALSQYARVELLQREQGS
                     PFYTAIYRLRERSLLVSRRIFPEPEGALLRGILLGERSGISAELQNAYSLTGTAHIIA
                     ISGFNMAVLAGLITGLFTRKLGAWRGGGLAILTLAFYTVLVGADASVVRAALMSSLGI
                     MGASINRRGSGLNSLGLVVCLMLLFNPHLPWDIGFQLSAAATVGILLFSAPMQARLRR
                     WLEARVGQAAARGLSDTAGEYLLTTFAAQALTLPLVLYHFQEISPLFLLANPLVLPAQ
                     PLVMLLGMLALLGGLLSLGLGKALAWLAWLPAAYTNRAVLWLAGLLPTAWRFPPFSFF
                     WVLAAWALALSLAVPLKHKPAKALARPIPLMIIGASLVVLVWSTAARAPDGQLHLRAF
                     NSPEQPVLLVRGAEGRYLLVGGSLPSASLKQQLGSALPPFQRELDALIIPACGKDDVQ
                     GLFGLSESFRIGAVYWACNPERLQTTRRLYETFQSAGVLQRRLQPQDELLLGGSAVLG
                     FTLGEDALKALSLRSADFSALVQFESAVNLEPVSLWLGAFAQEMPDAQARLAVGPRLL
                     TDLEQNPEISRVIALSEWTWVEAATDGHALRILAVK"
     gene            complement(27244..28500)
                     /locus_tag="PKL60_00125"
     CDS             complement(27244..28500)
                     /locus_tag="PKL60_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNW94571.1"
                     /translation="MSNVQPFLLVFGSSADPFHKGHSALITAAVRALSGRGWPVREVM
                     IMPVYRHHNTLDAVKRSLPFTYEHRFELCQLAAGDIARDLAGTVERVSVSRLEEEIAR
                     ESHRPNLSAETFTRLREECDPKLRLAFLLGTDSFSGEDPSLNHWFHLEALLGLTALVV
                     CPRQGFAPNLKFIGELEAKGAQIILLDEVSAPEISSSEIRRELESGAEPERLVQKGWL
                     SPAAAEYIRSNHLAESWRELDSSGRMPVEERQEGDPPGLEARIGKILGGHKWTLALAE
                     SCTGGLISHRITNVPGSSEYYIGGVVAYAYAAKVALLGVRWETLQRYGAVSSETVLEM
                     ARGARELFKTDFALSVSCIAGPGGATPNKPVGTSWVGLAAAGKEWSRHFLLSGDRAAN
                     KEMLAQKALELLLEFISTESSPGSKP"
     gene            complement(28497..30389)
                     /gene="nadE"
                     /locus_tag="PKL60_00130"
     CDS             complement(28497..30389)
                     /gene="nadE"
                     /locus_tag="PKL60_00130"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNW94572.1"
                     /translation="MGSSPAPQTKTPCRGLQLSVVVPELRAADVDFNCREILRALEQT
                     TAQNPAPRLAVFPELCLTGCSCGDLFLQPLLVESALRALERIAAGCRSMETWALVGLP
                     FQHVKRLYNAAVLLGPHGPAGLSLEREPLDPLSGFPSRWFSAGGEFPDTQAEILGQSV
                     PAAADLSLRLPELYPERIQLCVGRTENLILEMRVGLLLNPCALPALALADQTPETRLS
                     LRGRQAVWAYASCGPCESTAEAVFSGKAGILHDGETLAETQPLQFSTQSARALIGPRK
                     RSARKAAEQSNEAPPPALTQTPFLSVSQPDAQCAAAFAIQSAGLAGRLRRTGLRKVVL
                     GLSGGADSSLALMVSFQAFQLLGLEKQDILALSLPGPGSTQSSRERLERLASLCGVTL
                     REIPITGALESHLRDIGHPPDCFDLTYENAQARERTQILLDLANQHSALMVGTGDLSE
                     IALGWCTFAGDQTSNYHVNTGLPKTLVLRVLAWAGRELFGEDGAAAVSSICAAAISPE
                     LLPVRADGSSPQETETVLGPYLLHDFFLYHSLVNKLSPRQVYQLALSAFAGQYAPQQV
                     LAWLRLFYRRFFRQQFKRSAGPEGPKVSALSLSARGGWVMPADASGALWLNELENLDT
                     NPGENP"
     gene            complement(30489..31268)
                     /locus_tag="PKL60_00135"
     CDS             complement(30489..31268)
                     /locus_tag="PKL60_00135"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNW94573.1"
                     /translation="MKVSLNYSQALLNLMSAGAARVDAVEWVDKLPLSQIAESRAQFP
                     QVAFHFHPGRFHHTHSWLAHLRAYLQACPQSPHVSVHLAPLPWLWTSAYMHRGRLLPG
                     PLAGLSVRSFIRAVKWLQRNSELPVILENMPALHPNRYLFESEPSVINKVLEETGCKL
                     LLDLAHARIAAQARRMSPEAYLSALPLEKSVQIHLAGVRAGRDGRLFDAHETLTDEDY
                     RLLEWTLERVQPEWLTLEYFREDSLAVAQQLERVQEFASQA"
     gene            complement(31265..32053)
                     /locus_tag="PKL60_00140"
     CDS             complement(31265..32053)
                     /locus_tag="PKL60_00140"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW94574.1"
                     /translation="MTAASSPETVLDVRGLTISFVESDGSLDVLDDLSFRVEENSFVC
                     LIGPSGGGKSTLLRAIGGLLRPTSGQILFPLQSRGGSLTGMVFQKPNLMPWRNLFNNI
                     SLPLEMAGKSPTEIQQITRDMIELVGLRGFEKNFPHELSGGMAQRVAIARSLVQDPEI
                     LLLDEPFGQLDALTRERMGEELLRIWGSKSKTIVMVTHSISEALYLSDRVLVLTSRPA
                     KISLDLPVPLPRPRRQEMRYSAEFARLSRVIHQQLTRNETETCP"
     gene            complement(32031..33008)
                     /locus_tag="PKL60_00145"
     CDS             complement(32031..33008)
                     /locus_tag="PKL60_00145"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW94575.1"
                     /translation="MMKRLNPIFMLTVLLLTACTGAKPAQNGPLRAVTLNLTYIPNVQ
                     FAPFYAAIENGYFADAGLDVTLNYGNEADLIALVGSGKQQFMIASGEQVLMARAQGLP
                     VVTVYNWYKEFPVGIVSLAEKNITSPMDLKGKTIGLPGLYGANYIGFEAFAQNEGLRD
                     SDYTLQSIGYTQVESLVSGIVDAAVIYLSNEPSQLRAMGYQINTLRVADSIDLIGNGL
                     VTNESTIKEDPELVRAVLGALLKGIQFSAENPDETYLICTKYVDNLAEADEAVQKQVL
                     AESIKLWDVYPTNEDHTRRWENMQSVLLKLGLMSQAVDLKGVYDGSFLP"
     gene            complement(33023..33829)
                     /locus_tag="PKL60_00150"
     CDS             complement(33023..33829)
                     /locus_tag="PKL60_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW94576.1"
                     /translation="MKNLTDIKQRGYTRPGGSRALTVLLAAAAGVIVWQLLWMLLKLP
                     GFILPAPAVVWQKFLQVLGNGLLWRHLSYTLLEVLSGLLIGSLSAALVGYFLFKHPRA
                     ERFTSPYLVASQSVPVVAIAPLLVIWFGPGLFSKVLICALTVFFPVLVNTLVALREVP
                     QDLRELMRSLKASPGQTARYLELPASLPVFLGGLRVGATLSVIGAIVGELVGSDRGLG
                     FLINVGRGQYDTALVFVAVLTLMVLAASLYGLILLLESRLLAWQTWRKNA"
     gene            complement(34369..35946)
                     /locus_tag="PKL60_00155"
     CDS             complement(34369..35946)
                     /locus_tag="PKL60_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94577.1"
                     /translation="MNTKPKLPFPFYPLLFAAFPVLSLLGHNIQEVNLRAGLRPLLMI
                     LGGAVCVWLALLLATRSIQKAGLVSAWLFLLFYTYGRAYSAAQAAGSLFQVFGHHRIL
                     LPLWAVLAGVGAVLLLKYVKDSGNLSIILNVFSAFLLIIPVYQLITYEIRATGAGKGI
                     SQGGVAAAAEDVKLQPGALPDVYIIILDAHLRSDVLDATFHLDNTNFINQLTEMGFYV
                     PPCSMSNYAYTPLSMSSFLNMQYLQDISPDFAPPNNDTDSLFELIEHNAVRKNLESLG
                     YSTYSLASYQRIAWTDADHYYPLDPASLVSTEERVVFSKFERMLFDTTLVRILTDYKG
                     GVTTSDKIPLNYPYADHVRQQWTILKELKQISGFQGPKLVWAHLTMPHAPYVFYPDGS
                     LIPDPPELPWVVPLPWDQYLQYYSWGVQYTDNAILPVLEAILKNSDSPPIIVITGDHG
                     ADSANRLAVLSAFYVPDTVKAQITPDISLVNYFRVIFNGVYGANYEILENISYLSDKV
                     NTFGFTLYPETMPDCMP"
     gene            complement(36254..37639)
                     /locus_tag="PKL60_00160"
     CDS             complement(36254..37639)
                     /locus_tag="PKL60_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94578.1"
                     /translation="MKLTSSFRDPSGFMFHQDGVLYRQVNQSYQEDYDLLMESGLYQH
                     LSSARALISHEECSLDIAPVPGLAYKVLKPEPIPFVSYPYEWCFDQLKDAAVLTLSIA
                     RRALQYGMILKDASAFNVQFKNGRPIFIDTLSFERYLPGAPWIAYKQFCQHFLAPLTL
                     MAKVDIRLNQLLRVYIDGIPLDLTSSLLPKGSLLNPGLLSHIHLHAKTQQKYADMALP
                     EEQVSGKMTRNALLGLLDSLLSAVRSLKLKAQKTEWENYYEQTNYARASFEAKREKVS
                     AFILRVSPRSVWDLGANTGEFSRAASSAGIPTIALDIDHAAVQRNYALVKQNQEAQLL
                     PLCMDLTNPSPALGWNCAERMSLLERGPVDMVMALALVHHLAISNNIPLENLAAFFAG
                     LGHWLIVEFIPKSDSQVKRLLSSRRDIFPDYTEAGFKKAFGAHFEILEEAPIAGSERI
                     LFLMERKQKLS"
     gene            complement(37696..37938)
                     /locus_tag="PKL60_00165"
     CDS             complement(37696..37938)
                     /locus_tag="PKL60_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94579.1"
                     /translation="MNIFFAPIEKLTHYLDPGTGSMVLQVLLAALLSVGVLVKVFWRK
                     IRSWFNPNRNALRADLDPTEIVEPESTPNNPDDSLK"
     gene            38093..39280
                     /locus_tag="PKL60_00170"
     CDS             38093..39280
                     /locus_tag="PKL60_00170"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNW94580.1"
                     /translation="MTDKLAWIDDELKTLKDTGFYNSIRTLDSPQGAWLVVDGKKVLN
                     FCSNNYLGLANHPRIVQAASEAMQKYGVGPGAVRSIAGTMSLHLQLEKRMAAFKKVEA
                     AITFQSGFMANGATIPALVGKEDVIFSDALNHASIIDGSRLSGAKIIRFAHADPADFE
                     KQIQAAAGTYRRALMISDGVFSMDGDLAPLDKLVEIAERYDVMTMVDDAHGEGVVGEG
                     GRGIVDHFHLHGRVDVEVGTFSKAFGVVGGVVAGSARIIEWLRQRGRPFLFSSAVTVP
                     DTAACLAAVDLLEESTELVDRLWANARYFQAEMRRLGFDTGATQTPITPVMLGEVQLA
                     REFSRRLFDEGLFAMALGFPTVPQGMARIRVMLSAAHSQSDLDQALEIFRKVGKELGV
                     LRG"
     gene            complement(39343..39424)
                     /locus_tag="PKL60_00175"
     tRNA            complement(39343..39424)
                     /locus_tag="PKL60_00175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39388..39390),aa:Leu,seq:caa)
     gene            complement(39475..40278)
                     /locus_tag="PKL60_00180"
     CDS             complement(39475..40278)
                     /locus_tag="PKL60_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94581.1"
                     /translation="MMKKISLIAALLLSVGLACSMGRNPAPTATIAAAPTLGLVPTEV
                     PTTSPVPTETSAALAPTEPILIPSPTAKSAVPLSTQVGLPEQGEILLEDDFSTTDKAW
                     YTGEEDGNSIKNLGGIMEFTVVKESWLIWTESTLLDTPDVVIEVDALLWDGDLTNNYG
                     VMCRYVDEDNYYSLTIANDGYVEILKYVGGEAELLYGEMQLAGFDENLNHIAASCIGE
                     TLSLYVNGDFIASVEDSSLESGDVGLVVGTYDVPPVTVRFDNFIAYTPR"
     gene            40478..41548
                     /gene="ychF"
                     /locus_tag="PKL60_00185"
     CDS             40478..41548
                     /gene="ychF"
                     /locus_tag="PKL60_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNW94582.1"
                     /translation="MQLGIIGLPQSGKTTLYKALTRADIPTDISSGQMEVHTAVVDVP
                     DPRVDQLCEIYHPKKIVRAKVTYADIAGLDGSAGKNGLSGALSNMLSQMDGFLHVVRQ
                     FENPSVPHQAGSVDPIRDIQTMDTEFVLNDLVMVERRLERLREEHRRGVTRDRALIQE
                     DIDLFERLQAVLEKELPLRHYAFTPEEDKLLSSFQFLSRKPQIVVFNQSEDQTPLEYH
                     SPHPHTSSVSLPAKLEMDIAALDPEDAAVFMAEYGITEPSLSRLIRLSYDLLGVHSFF
                     TAGDKEVHAWTLRKGSNAREAAGVIHSDMEKGFIRAEIISFEDMITFGGTAEARAKGR
                     LRVEGKTYIIQDGDIIEIRFNI"
     gene            complement(41631..42467)
                     /gene="surE"
                     /locus_tag="PKL60_00190"
     CDS             complement(41631..42467)
                     /gene="surE"
                     /locus_tag="PKL60_00190"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNW94583.1"
                     /translation="MKTNEKPQILLTNDDSINSPGLWAAAEALAPLGFVHVVAPRVQQ
                     TSMGRALPRESDGIIEARTMTVNGKEWQVYAVGGSPAQAFLHGYYEVLGFKPDLVVSG
                     INYGENVASGITISGTVGAALEAATLGISALAVSLETPVSQHFNLSPDVDFSVAGYFT
                     RYFAEKLLRTAMPFDVGVLKVDVPNDATPETPWVVTRQSRERYFYAVPADPDKAQGSR
                     KLTYEVRLDQNEPEADSDLHVVKIDRKVSVTPLSFDLTARVNLSDFASLLRQNNPPEF
                     AR"
     gene            complement(42571..43089)
                     /locus_tag="PKL60_00195"
     CDS             complement(42571..43089)
                     /locus_tag="PKL60_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94584.1"
                     /translation="MPVSSYRMLNIGGLPTGLLGLEELFAELYAEGWLPTDEKLKDAL
                     IAGVRKQNFIPTPAVPEYAGALCMEYSRFFRLKASGKAAVARDYGSWRGYPREQIPWF
                     PAIAPELCNDCGACLETCAREVFERDENGHICVAEPFLCLVGCCFCKSVCEPKAIRLP
                     GQEILKNYREKM"
     gene            complement(43089..43517)
                     /locus_tag="PKL60_00200"
     CDS             complement(43089..43517)
                     /locus_tag="PKL60_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNW94585.1"
                     /translation="MQTTAEIAAQISAEFSLLENWEEKYDYLIEKGQSLPDMPKDLKD
                     DEHLVKGCQSSVWFVTECREGSLYFQADSDSLIVKGLAALLQELLNGQPAEAYQNVDL
                     SLFETLGFWRHLSSQRSNGLTAMVSFLKTAAGDCAAQRGE"
     gene            complement(43522..44748)
                     /locus_tag="PKL60_00205"
     CDS             complement(43522..44748)
                     /locus_tag="PKL60_00205"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNW94586.1"
                     /translation="MSTPISAARADFPILSTWINDHPLVYLDNAATTQKPQVVIDSLR
                     DYYSFVNSNVHRGGHTLAVRATDMYEAAREKVARFINAAHAHEIIFTRGTTESINLAA
                     SSFGEAFVHPGDEIIVSQLEHHSNYVPWFELCQRKGARFRVLPFTPTGELDPEVLQGM
                     FTEKTRLLALNQVSNSLGTVNPLETLIPLAHAAGVPVLVDAAQSVQHLAARDVQTLDA
                     DFYAFSGHKMYAPMGIGVLYAKESWLEKLPPFLYGGEMIDQVSSTRVTFAQLPHKFEA
                     GTPNVEGAIGLGAAVDYLNQFDPQELQTYEEDLLAYGISQLRAIPGLTLYGNPKHRSS
                     ILPFNVEGIQHYDMGILLDTSGVAVRTGQHCTQPIMDALSISGTVRASLALYNNRDDI
                     DALTRGIRRVIKMLKR"
     gene            44929..45639
                     /locus_tag="PKL60_00210"
     CDS             44929..45639
                     /locus_tag="PKL60_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW94587.1"
                     /translation="MPEKILVVDDEISLQETLAYNLTREGYQVEVAGDGNQALHLART
                     SKPDLVILDVMLPGLDGFEVCRILRQEANLPVLMLTARDDEIDRVVGLEVGADDYLAK
                     PFSMRELIARVKALLRRVRLTREDMVTEKQEAEQPKLITFDNLVIDLTRREVKLDDKV
                     LPLKPKEYELLLFMGQQKGRLLTRELILERVWGWGFVGDSRTVDVHIRWLREKIEPNP
                     ETPQRIVTVRGAGYRFEG"
     gene            45639..47060
                     /locus_tag="PKL60_00215"
     CDS             45639..47060
                     /locus_tag="PKL60_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW94588.1"
                     /translation="MMGLLSKRRFILPYAFILIGSILLSAVLVPPAVRSAYARSAESG
                     LPPDFTLMYLSIGAVGLLALAAAMIANRIYANRNLNIIRQLTVTASQLGEGKYRSIAI
                     PHQVESVPEMHALSQALEETAAKIEAQISALSSEQVMLSTVLGQMTDGVLIANHDDRV
                     QLLNPAAERLFGFKNAGALGRSVIEVIRHHALVDLWNSTKEGESKTITLEMGADHKFL
                     QVIGIPLGEQLPERSMLLFQDLTQLHRLEIVRKDFISNISHELRTPMASLKALSETLL
                     DGALDDPSVAKRFIVRMDTEVDNLTYLVNELLELSRIEAGRTNLEFQRIHPCDLMRRP
                     YERMLLQAERAGLSLTMNCNPDLPAVLADPDRISQVFINLLHNAIKFTPNGGRIQMDA
                     WVFEDKVVFMVRDNGVGMPEKDLKRIFERFYKVDRARSGGGTGLGLSICKHMVEAHGG
                     YIWAESEEGKGSSLFFAIPIAFG"
     gene            47249..47935
                     /gene="phoU"
                     /locus_tag="PKL60_00220"
     CDS             47249..47935
                     /gene="phoU"
                     /locus_tag="PKL60_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNW94589.1"
                     /translation="MPRLTLDREIRLIQDETLLLGSLVEQAVLNAVDALKSRDVSAAR
                     AVIRRDVLINDKRFAIENRILILFATQSPMARDLRLLAAELELITELERIGDYAKGIA
                     KVAEELADDETPVPIREICTMADLAVSMLHRALSAFIAEDLDTARALPAEDDQVDELY
                     KVIYRKIVQGMIANPSSIDHSNQILWVIHNIERTADRVTNICERIVFIATGELLEMDS
                     SDDELETPEA"
     gene            48076..48801
                     /gene="tmk"
                     /locus_tag="PKL60_00225"
     CDS             48076..48801
                     /gene="tmk"
                     /locus_tag="PKL60_00225"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNW94590.1"
                     /translation="MLKPNTTKGKLIVVEGIDGSGKSTQIDLLFKWLQSQGYSVFFSE
                     WNSSPLVKSTTKAAKKEKSFTPTTFSILHCTDFADRWENSIYPLLKAGAIVLADRYVF
                     TAFARDVARGVDPEWVRNMYSFAIKPDAVLYFRVPLEVAVERITAARASLKYYEAGMD
                     LNLSENINESFKLFQGKILEQYDQMVDEFGLTLIDGTQPVKDQQKKVRALIKKILRGW
                     EGLPNPNKPAGSARPAAETGGKS"
     gene            48798..49511
                     /locus_tag="PKL60_00230"
     CDS             48798..49511
                     /locus_tag="PKL60_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HNW94591.1"
                     /translation="MKGANFYGAGFPYRDIQQLPGKLIVLEGTDGVGRSTQIKLLRRW
                     LEDEGYAVSDTGLTRSALTASGLEAAKSGHTLGPLTLSLFYLADFADRLENLIIPALQ
                     AGFVVLSDRYFYSVMARDAIRGVDKSWSRQLYGIALRPDLILYMKADVPVLAQRLIHG
                     RGLNYWEAGMDLHLADNMYDSFLAYQNLLTKEFDELALTYNFVTVDANQKPDTIFEDL
                     KVHILELLHNRLTKTKKPR"
     gene            49571..50608
                     /locus_tag="PKL60_00235"
     CDS             49571..50608
                     /locus_tag="PKL60_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNW94592.1"
                     /translation="MNANADAITVYGAKIISRHAAAMAAEMEGVRKAEDIEYIHRMRV
                     ASRRMRSALTIFKGCFDKKKFNEIDRDVRKVVRALGEARDLDVQLEVVREAEAQFNTP
                     RLLPGLKRLQLRLTQKRAQAQQHVVSAMDRLAQDQLLERLEAWYAPLQAAAEGVYLYS
                     PALYQLAFESVQARLKELLGHVPYINDPANVAELHAMRISAKRLRYTLEAFDDLYGGR
                     LKPFISTGRKLQDQLGLIHDLDVWIFFVPAFIEEERARIVGYFGSDGPLKRLLPGLLT
                     FQKSRREMRDAAYAEFLVSWAEIEAAKTWEEMMRLLNTPMDLDSALRLLEAARAGQNP
                     EAQPGSTAADD"
BASE COUNT        10777 a        14884 c        14752 g        10329 t
ORIGIN      
        1 gagcagctca cgaggctcat ccagactgcc ctgaacgagc gcggggtcca ggcgcctgag
       61 attaccatcg aattcaatcc cgaaaccgcg ccaacggaaa tgcttttcaa tcagggcatg
      121 atgattgaaa agatgccgga ggaaaaacgc gccccgtacg aagcgcgcct gcacgaaatc
      181 aaggttgtgc tcatccgcag tctcatcagc gaccaactgc cgtacatcaa tatcgccaaa
      241 gactggttca ccgtctccga cctggcggaa atccgtcagc acaagctcgg gcgcggccgg
      301 attggcggga aagctgccgg catgctgctg gccgcgcgca tcctgaagga aaaagccggc
      361 ctgcgcttgc gcgaaagcct gcaaacgccg acctcatttt acattggctc ggatgttttc
      421 tacaatttcc tgtccatcaa caacctgcat cactggaacg accagaaata taaagacgaa
      481 gagcaaatgc gcagcgatta cccgcggatt gtggaagatt tcatccgcgg agactttccc
      541 ccgacggcag tccagcacca ggagaccatc ctgagcatgg caggcaaacg cccgctgatt
      601 gtgcgctcct ccagcctgct ggaagataat tttgggacgg ctttcgccgg gaaatacgag
      661 agcatcttcc ttcccaacca ggcagaccct gaagagaacc tgcgcgcgct ccagcaagcc
      721 atcgcgcgca tttatgcttc cacgctcaac cctcctgcgc tgctttaccg caaaagccgc
      781 ggtctgctgg actatgacga acgcatggcg ctgctcatcc aggtggtgga aggacagcaa
      841 atcgggccgt attacttccc gcagttggcc ggcgtcgggt acagccagaa tcagttccgc
      901 tggtccccgc aaattcgccc gcaggacggt tttgtgcgcc tggtgtgggg tttgggaacg
      961 cgtgcggtag accgcgtggg caacgattac ccacgcctga tcgcgctcag ccatcccacg
     1021 ctcacgcctt cctcgtccgc gcagtccatc cggcgctatt cgcagcagtt tatcgatctg
     1081 attgacctgc agcagaaccg ctttaccacg ctgccaatcc gcgcagtgct cagccagaaa
     1141 gtcccgcaga tgcgcttcct ggctcaactg gacagcgaag gctacttcca gaccctgcgc
     1201 agcaacctgg tggagggcaa tcccgagaat ctggtgctca cctttgacga gctgctgcgc
     1261 cgcaccgatt ttccacagtt gatgcgcgag ctgcttgcca ccctggagag cgagtaccac
     1321 acgccggtgg acattgagtt cacggcgcgc gtggcggaaa atgacggcag tccggcagtc
     1381 caaatcaccc tcatccagtg ccgtccgctg ggaaccatca aagaaggcaa atacgaactg
     1441 ccgcagcgcg tggctgataa tgacaaaata ttttccggga tcggcgttgt gaccggcggg
     1501 atgattgaag gcgtccgcta cgcgctttac gtcaaaccgg acgcgtactt tgccatgccc
     1561 acccaggaag aacgcaatca gctggagcgc gccatcggca aaatcaacgc cgcgctgcac
     1621 ggcgaaaact acatttgcgt cggacccggg cgctggggca cttccaaccc ggacctgggc
     1681 gtgcacgtgg attacgccga catttttaac gccagggcga tcgtggagct taccggcggc
     1741 gcagtgggga ttccggacga accctcgctg ggcacccatt tctttcagga cttgctggag
     1801 gggcagattt accccctggc ggtcaacctg aaccgcgaca ccttcaatac ccggttcttt
     1861 gaaagcacac ccaaccacct gctcgagtgg ctgcccgacg cggaagccct ccaacactgt
     1921 ctgcgggtca tcagaacagc ggattacagc ccggaaagcc gcctggatat cgtcatcgac
     1981 gatctcaacg gcagcgctct ggcgttttta agcggggaaa cggtaaaagg cggcgagaaa
     2041 aggcagctta tagtgccctg aatgtttcac gtgaaacaaa tatccggcgg ttggatgttt
     2101 cacgtgaaac atggttaggt tgaggggcgt tcctcagcaa gcatgaataa agtccgcgcg
     2161 ctggttagcg tcctgttttc tttcgtgatg gcaggccagg gaattgccgt gcctggtgca
     2221 aataagtgat acctgcctga ttgatttttt ccaaaaggaa atgcgctggt agggcggata
     2281 agggtcgttt gtccgattca ttacagacaa attcagatcg aaaagcggat ttgccaatat
     2341 cagtcacgct gcgctggcga gtttcccttt ttggtatgta agcagtcggg agcaggggca
     2401 aagcaaccca attaatccca aatctcaggt ttggggttga tgaaggatgc ttcgtgccaa
     2461 catggtaaag gcgtattttg gttgcttgaa taagagtcta tggacaatca aagccctcat
     2521 ccgtcttgcc ccgctcgcaa gcagccctct ggggaaggga aacaggcacg tctcgccttg
     2581 ccagcttaga ctttgattca agctctaaat ttcccttgac ccaatcgcgg tttagggata
     2641 aaatctctcc cctcaaagca atttgccctc gtagcttaat ggatagagca cctgcttgcg
     2701 gagcagtagg ttgcgcgttc gaatcgcgcc gagggcattt tcctttttta tcacggttct
     2761 tcattttgtc cgcgccctac gcagtggtag aatgctatta aggagcgcgc gatgaaaaaa
     2821 cacttgttcc cctatctttt cctcctgctc gcggcgcttg cgctggctgc ctgtgcgtcg
     2881 aaagccgcac cggaagcagt tttgcccgag tccgcctatc ccgccggcgc gaatgcccgc
     2941 acggtttacc ctatgtccac tcccaccagc acaagcgcac cggcaaccgc ggaaagtccc
     3001 ggcgccgttt cccccgcgcc aattccgctc ttttccatcc gaatcttccc ggatgggcgc
     3061 ggttgggctg ccaactggaa ccaaaccgcg ttgtaccaca ctgccgacag cggcctaaat
     3121 tggacggaag tcagccccgc ggacgccatt tccggggcga gcggcggttt ctacagtttc
     3181 tttctggacg gcagcacagc ctggctcagc ctgccgcttt ttgaaagctc cagcctgctg
     3241 cgcacgactg actctggcac gacctggtcc acgacccctg tggatttctc cgcgggcaat
     3301 ccctggtttc tggacagtca gaacggcttt ttgctcagcc gcctggatgc cgctgccggg
     3361 tctgagttcg tggggattta tcaaaccgca gacggcggcc tgagctgggt gaagcgcttc
     3421 agtcacgagc ccggcatggc agacccctcc ctcccggctg gcggtcaaaa aagcggcttt
     3481 acgtttccga acctgattaa tggctgggtt gctggaagtg agcctattaa tgacttcatt
     3541 tatttatacc gcagcaccga tagcggcgtg aattggctac tgcaagatgt tccgcttcca
     3601 gatgatgcaa tcggagcaat gttcgaaagt ttaccgccag tgttttttaa cgcttccgaa
     3661 gctgttctgc cagtgcgtat ttatcttccg gacggggatg cacacaccgg attctaccgc
     3721 tccactgacg gcggcgaaac ctggtctttc ctcatcggcg cgctgccccc ggcgaaccgt
     3781 tttgtctttg cggatgcaca aaactggtgg ctgctggggg aatccacttt tctccgcacc
     3841 ggagacggcg gcctgacctg ggaagacctc tcggcagcgc tgccagtcgg ttttaccccg
     3901 cttaacctgg attttgcgga cgccaaccac ggctggctgt tggtctcccc tgttggggac
     3961 cttgagctgg agagtaattt tctctacggc acaagcagcg gcggcagcgc ctgggaactg
     4021 ctgcccgcgc gactcacgcc ttagccgccc cgttcggata aacgctgtat tggcacaggt
     4081 cctgcagcga ttcgtaaaag cttttgcccc gactggcttt gaaaagcgcc cggagtctat
     4141 ttataatgag gataagtgaa ggttcgcaag atgaaaaagt gcctgtttag catcctaaac
     4201 ctggctgccc tggcagggtt ggcgctcact gcccccgcac ccgttttggc agcagctcag
     4261 gcacaagccc tgccgcccgc cagtcagagc gccgacccgg gtcgcaccca atcttttatt
     4321 caactcgtga acaactggac cgcgccggac cagccggctg attccgcgca ggacctgccg
     4381 cacgcgaacc ccgacctggt ggtctacctg acttttgacg acggcccgga ccctgattgg
     4441 actccacaga tacttgccct gctgcaagaa taccacgcga ccgccgtgtt ctacgaaatc
     4501 ggccgcaacg cgcgcacata ccctcagata tctttggaag tagcggaagc cggccagatg
     4561 atagggctgc acggctacaa ccacatcgaa ctctccaaac tgggctataa cgatttctac
     4621 ctggaagtta tcgatacccg ggacgctatc caggatgcgc tcagcagccg gccggacctg
     4681 gaaggacagc ttacgccctg cctgcgcccg ccctacggcg cggtgaatga tgcggtatac
     4741 agctacgcct acaacatgaa ttacgcaatt tccatgtggc acctggatac gcgcgactgg
     4801 accggaatct ccggggaaga aattctggct accgtggaaa aagaactggt accttaccgc
     4861 gtggtgctga tgcacgacgg cggggaggac cgcagcgaga cggtgcgcgg gttggagctg
     4921 gtgctgcacg agctcaccct gcgcggatat cacttcgagc cttattgcac agccacaggt
     4981 caggaataca atcactgatg ttccggcagc atcgattgtc gattttctta tgaaattcaa
     5041 atgaacccag ccgtcttttt ttccaatttg acttgatttt gttttaattt accgttatac
     5101 taaatatgtt agacgcttgg gtgcccccct cacccaggcg tctaacactt taaccgcgga
     5161 ttccccggtc ccaggccgct gaaagggttg aatttggata tctggtttat gcaatgtcag
     5221 aacccaggct gtcagctgcg gttccccctc gatttggcac agtacaaagg caggtattgc
     5281 ccgctgtgcg gcgctcaact cgaacgcgca caaaaagcgc tcgagcccgg cgcgccggag
     5341 caggattaca gcatcaacaa aggcgcgctg gaaggcttgc ttgataacat ccgctctgcg
     5401 ctgaatatcg gagcggtgtt tcgcgcggca gacggcgccg gcctgcgcaa gctttggctg
     5461 accggcatca ccccccgccc cacgcagcag gcgcaaattg ccaagacagc cctgggcgcc
     5521 gagcaagccg tcccctggga aagcgcccca aacgcgctga ccctggcggc cgacctgaaa
     5581 gcgcgcgggt acaccctgct ggcgctggaa aacgcgccga acgcgcgccc gctctttagt
     5641 ttccggtcgg acgcgccaaa gcacagcccg ctgctcctga tcgtcgggag cgagcaggct
     5701 ggcgtggacc ccgggctgct ggcgctggcc gacgaaacct tgtccctgcc aatgcacggc
     5761 agtaaaggct cgctcaatgt cgcggttgcg ttcgggatcg ctgcatactg gctgcgctat
     5821 gctcctctaa cgtagaagag gctgtccaaa tagaacgggt ttttcttttt gggcagccca
     5881 aagcggggga tagcagctgg tttaggcgtc ttgtaaacag cgcaaatccg cgaacaccct
     5941 aaaaaagggc acggagtagg aaatcagaag gggccgtccg tctttttgga cagccccttc
     6001 agtgcgtgcc tggtaagtgt ctacggcgcg atgaccgtcc cggccgggat cgtggtatgt
     6061 ttgggaatta ccacaatccc atcccgcacc gtatagagcg cgtggtccac ttccatgtcc
     6121 accgggaagg gtttgatgac cacgttatcc ccgatactgg agttcttgtc gataattgcg
     6181 ccctgaatcg tgcaattttt gccgatgccc agcggcgcgc cgttcttcag catgccgtaa
     6241 tagtccccgc ccatcataat ggtatccttc accacggaac ccgcgccaat ctggctgcgt
     6301 acgccgatca cggaatgttc aatcgtagcg ttttcgatgt tacagccctc cgcaatcagc
     6361 acctgcttaa aggagcagcc gaccatgcgc gagcagggta aaaagcgcgg atgggtgaaa
     6421 atcggtttgt caggatcgta aaaatcaaat ccgggctgat cgtcggtcaa ttccaggttg
     6481 gtctcgtaaa atgagcggat tgttccgata tcgcgccagt agccctcgta gttgtagccg
     6541 taaacggacg cgccctgccc gataaggtag gggataatgt cgccgccaaa gtcgtccagg
     6601 cgctcgccgc ctttgcgcag caccttcacc agcagttcgg tattgaacat gtagatgccc
     6661 atcgagccga gataaggact ttccgggtcg tcccggctga ccatgctggc cagtttgatc
     6721 ggatccttgg gtttctcagc gaaatcggtg atgcggccgt cttccgcgcg cttgagcaag
     6781 ccgaagcggg aagcctcgga tttgggcaca ggctgcacag cgacagtaat atccgcgttg
     6841 ttatcccaat ggaactgcgc catctcagcg taatccatgc ggtaaaggtg atcgccagcc
     6901 aggatgagga cgtacgggca cttggtggag atgatttcca tcagctgctt gcggacggcg
     6961 tccgcggtac cctggtacca gtcggtatgt tccatggttt gttccgccgc ccagatttgg
     7021 acccaaccgg ggtggaaaga gtcgaacaga taagtctggg aaatgtgcct gtgaagtgag
     7081 accgaattaa actgcgtcag taccgcgatg cgaaaaatgt cggagttgat gcagttgctg
     7141 atggggatgt caatcaggcg gtacttcccg gcaattggca ctgcgggttt ggaacgttcg
     7201 agggtgagcg ggtaaaggcg tttgccttgt cctccgccga gaatgacgcc gagtatctcg
     7261 ctggtcttgg gcataatggg tatcctttct tccgaatcca tttccgtcag gaacttaccc
     7321 ctgcgtatga tgatggcagc agtccgattg tggtgaactt agcggactgt attaatttta
     7381 cccgtaaatc cttccaccgc gtgtaataaa tcgcgcgcgg actgcagcgt gccggcgctg
     7441 accgagccaa tcatgcccgc cagttcctgc tcgcgcgcgc cagcttcaag ctcgctaacc
     7501 tgcgtcaggg tgcggccgtc tgtttcgtgc ttggagacgc gcagatgctg gtcgccgtag
     7561 gctgccaact gcgggaggtg cgtaacgcac attacctgat gcttgcgccc aagctgccag
     7621 agcatctgcc ccacgatgac gccgacccgg ccgccaatac cctggtcaat ctcatcgaaa
     7681 accagggtgg gaatctggtc ggcttccgcc agcacatgct tcagagcaag catcaggcgc
     7741 gaagtctcgc caccggaggc tgtcttcacc agcggcttca agccctcacc cgggttggtc
     7801 tcaatcagga attccacccg gtcaaccccg gctgcgtcaa aagccaggcg ctgaccgtca
     7861 acctccaggc cttccgggtc catcagcgcc gagaagctta cctgaaagcg ggcgtttttc
     7921 atttgcaggc tttgcagctg ggtttccact ccgttttcaa gcttttgcgc ggcactgcgc
     7981 cgttcagcgc tgagcctggc agccagaact gcaagctctt gcttaatccg gcttattttt
     8041 tgctccactt cgcttatctg gtcttctaca gtctcgactt tgcttaactc gtctttggct
     8101 ttctgcagat aggcctttac gctttccaca ctgccgccgt acttgcgctt gaggaagttc
     8161 agggtgttca gccgttcctc aatttggtcc agccgcgcgg ggttgaattc gatgttctcc
     8221 aggtatccgc gcagttcgta ggcaatttcg gatacggtgt tcagcgcctg gaccgcccgt
     8281 tcgctcattg cactcatttg cggatcaata cgcgccagtt cgttcagggc gtgctgcgcc
     8341 tcgcccagca ggtcggtgac cggcagggcg tccgcgccct cgtccaaagc ggagagcgcc
     8401 tggcgcgaaa ggctgtgcag ggtttcggcg ttcgccagca aggtgcgttc ctggcgcaaa
     8461 gcgttctcct cgtctgcttt caggcgcgcg gattcaatct cctgaatctg gtacttgagc
     8521 atgtcggtgc ggtctttggc gtattgctgc aggttcagga gctcttccag ttcagagcgg
     8581 tatgccagcc actgccggta aagccgggtg tactcgctaa gcgcgtcttc gtgatgcgcg
     8641 aagcggtcca gcagctccag atggcggcgt actttcagga gcgaaagatg ctcggactgc
     8701 ccgtggatat ccgccagcag agagccaagc tcggattgca gcgagagcga gacgctgcgc
     8761 ccgttcaccc gcgccacgct gcgcccgcct gcgcggattt cgcgcgagag cgagagcgtg
     8821 ccgtcctctt cccacaggcc ttccggctcc agcagcgccc ggacgcccgc ggcggtggga
     8881 gcgtccaacg cgaaagaggc ctcgatgaca gctttgtcac tgtcccgccg gatgtcgttc
     8941 acgtccacgc gaccgcccag cacagcgttc agcgcgtcca ggataatgga tttgcccgcg
     9001 ccggtttcac cggtcaatac caccagcccg ttcgaaaagg tgagggttaa ttcctgaatg
     9061 atggcgaaat tttggatgcg cagttctgca agcacggcgg acctcagttc aacttgatgc
     9121 cggtgctttg atagtaagcg ctggagacca gcagcagggc gtaaaccgcc tgccagacca
     9181 gcggaagcag cccgaccagg gcagcctgag cgccgttagc gccaaaaatc ggaagcaggc
     9241 gcaggatgag caaaggcagg catcccagga tgctgcccca cagaagcgct ttgttcagcg
     9301 cttggctgcg gcgcttgcgc acagcccagc gcaccacgtt ggcaatcagc gtgcctattc
     9361 ccggcgcgac gaacaaaatc agaaagccga tgcgcccggc cagccagctg ccgccaaagg
     9421 caatcagcgc ggaaatcagc agtcccacgg gtacgtccag gcctttattg gtgtcgtaaa
     9481 tcttttgctg ggagcgcacg cagtccttgc agcggtaacc ggtgggcgtc agcacggccg
     9541 tttcgggtgt gataggtttg ttacagcggt tgcagcgcag aacgatttct tcctgcgcgg
     9601 cgttgggggt aggtaggttg gtttcagtca tgatggtttt gtttcagtgc agggttatca
     9661 tacagcacgg ttgccagccg gcggtagaag taattcggtt cgccaaagcg caccatcttc
     9721 agggaatgtt gattcgccgt cacgcgcacg gcgtcgttat cgttcaggcg gatggggtca
     9781 atgccgtcca cgctgatgac cgcttcgtgg tcgcagtgag cgcgcagtac cacggaagcg
     9841 ccttctgcca gcaccaccgc gcggtccagc gataggtgcg gggcgatggg cagcagcagc
     9901 atgttgcgca gttccggcgg caagatcggg cctccgacag ccagcgcgta ggctgtggaa
     9961 ccggtggcgg tggcgacaat cagtccgtct gcaatgtagg aggtgaggta cccctcgttc
    10021 aggtccacgc gcacctcaat cgggcgcacg atgcggccgc gtgagacgac aatttcgttg
    10081 atgacgtccc actcgccgca cacgcgctca tcctgaatca actgggcaag cagcatcatg
    10141 cgctgttcca ggtggaattc gcccgagagc aagcgcagca gcgcctcttc ccaggtggat
    10201 tcggtcagtt cggagaggaa gcccagcttg ccggcctgga cccccaacag tggaatgcca
    10261 agcggagcgc acaggtggcc ggcgcgcagc agtgtgccgt cgccgcccag gacgatgacc
    10321 aagtccgcgc tgctcaggtc cgggcctgag gggatggcgg caggcgcggc gacgaaagtg
    10381 cttaccgcag cgcctgcccg ggagagccgc tcggagagga ggccggcaat ttgcagcgct
    10441 tttggcattt ccgtgttggg aagcaggaca atatgcgagg gggcggagac agttgaggcc
    10501 atagtcagta ttatagccgg agttgtgggc ttttcagacc cttgcagagg atatccgaag
    10561 cttggtggac atggcaacgc catccctgag cgaattgacg gagttttgtt attctgagcc
    10621 ggcagcggaa ctggtagttt gaaactaaag attcttcgcg ctgctcagaa tgacaagccg
    10681 gataagccgg gtgcagaggc gcagaacccg acgacagctc cacaaacccg ccggccaaag
    10741 cttgtcgggt tgcgctgccg ttccaacaaa cctgactttg ctgccgctcc acccgaccta
    10801 cgactttgct gccgccccac cggacctacg gccacgtgct gtcgggttgc gctgtcactc
    10861 cacccgacct acaaccacaa gctgtcgggt tgcgctgccg ttccaacaaa cctgactttg
    10921 ctgccgctcc acccgaccta cgactttgct gccgccccan nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnng ctgtcactcc acccgaccta caaccacaag ctgtcgggtt
    11101 gcgctgccgt tccacccgac ctacgacttt gctgccgttc cacttaacct acgaccacgt
    11161 gctgtcgggt tgcgctgccg ttccaacaaa cctgactttg ctgccattcc acccgaccta
    11221 cgactttgct gccgccccaa ctgacctaca accacgagct gtcgggttgc gctgccgctc
    11281 cacccgacct acgaccttcc tgccgcccca ctcgtaaccc gccctgactc tgcacccagc
    11341 ctttcaggat agcaccagcc ggaaagcctg atgatttgta cgatttcaca ggtttcccaa
    11401 ccttttcctc tcgaactctg ttaaaatcat tttgaaacaa ccagttagcc tggagctttt
    11461 ccatgcataa acgcctgaat atcctgctcg cctgtgtgct ttgccttctc cccgggctgg
    11521 ctcgccccct gcccgccttc gccctcccgc cagcacccca aagcacactg ccggacttcc
    11581 cctgttaccg cgaccttgcc accctctatg ccgacgcggc cgccctcgcc agcgcttacc
    11641 ccgcgctggc cacctggacg gatatcggcg attcctggga gaaaactcag cccggcggcg
    11701 ccccaggtta cgacctgttc gcgctcaggc tgaccaacag cgcgctgccc ggggagaaac
    11761 ccgcgctcat tctggtcagc ggcacacatg cccgggacct ggcgccagtg gagctgaacc
    11821 tgcgtttcgc tgaatatctc ctggcaggct acggctctga ccaagatctc acctggctgc
    11881 tggacagcgc tgaagtgcac atcatcctca gcgccaatcc cgacggccgc gctgtggtgg
    11941 aagcgcaaat tgccagcggc gaggcagacg aattcggcga taccgcccgc aaaaagaacc
    12001 tgaacaacac cgcctgcccc gatcccatcc tcaccggagt agacctggat cgcaactttt
    12061 cgtttcagtg gcagctgggc cccgacggct gcatgagcga ttacccgggc gccgcggccg
    12121 cctctgagcc ggaaacgaac gccctgattg cctatctgga gaacaccctg gaggattacc
    12181 gcgccggcgg cccggacgag ccggccgacc ccaacgcgcg cggtttgctg gtgcacctgc
    12241 aaagctatgg aaacaaggtc ttttacccct attcctaccg ctcggagccc gcgcctgagg
    12301 atgccgcgct gcaaatgctg gctaacaaat tcgcgtacgg cacgcccgcc tcgccggcaa
    12361 aatattccgg gacctacgcg tccttcctgc ccggcagcct ggcggattat gtttacgggg
    12421 aaaggggcgt gcccgcgctg gcgtacatgc tcttcgcgaa tatggatggg cgttacttca
    12481 cccgctgcag cgtctttggg aattctctct ccgccaacct gcaggcgctc acgcgggcat
    12541 tgaaaaccgt ttgggcgcct tacatatttc cagccgggcc ggaaatgacc ccgctgctga
    12601 ttactgtcca tcacagcgaa tctgaaacct ggtgggaact tttgacgagc gcggacacga
    12661 acatgctgaa ggaccccgac cccaacgcgc cgccggtggc ttccgcggtc tacagttttg
    12721 acctgcctcc ctgggaaccc ggcgcggttc tgcacgcgct gccaacccag gacggcgctt
    12781 ttgacagccc acaggagcag ctgcactccc gactggactt cagcgggctg gagcccggtt
    12841 ggcatcgggt cttcgcgcag gccttcctgg cggacggcac gccgggcttg gctgccgcgc
    12901 aaagcgtcct ggtgcccgag ccgcccccaa cgacccctta cagcagttac ctcccgattt
    12961 tattgcgcta acccctcaga gcttcattcc gagagcagat acgccaggat ccagttggtt
    13021 gtggcttcca gcgcctcgcg gtgcgtgcgc tcgtaatggt gcgaggcatc cactcctggg
    13081 ccgatgagcg cggcagccgc ggcgccgccc gcgtgccagt aagcggtagc gtccgagccg
    13141 taattggggt agatatccac tttgtaaggg atgacatagt tttccgccag cgcgcgcagc
    13201 ctgttgctca ggccgttgtg gtagggtccg ccgctgtcct tgacgcacag cgtagcatga
    13261 aattcgtccg aagcctgacc ttcgcccatg gccgccatat ccactgccac caattcttcc
    13321 acgctggcgg ggatatccgc cgcgccgccg tgcccgactt cctcaaaatt gctgaacagg
    13381 aagcaggtac tgcgggcggg ttcgaggcct gcagcgcaca gcgcctggat ggccgccgcc
    13441 atgcaggccg cgcaagcctt gtcgtccagg tggcgcgagc gcacaaagcc gttagtcagc
    13501 tccacgcgcg ggtcaaagga aacaaaatcg cccacctgga taccgagcgc gcgggtttcc
    13561 tcggcgctgc tggtgcgcgc gtccaggcgc acttccatcg tgtccgggct gcgtttctgg
    13621 tctttcacgt cgctgtagac atgcacggag gcgtgattgg gcagcaggct gccgcgcacg
    13681 agcacgccgc tttgggtgtg cacccaaacg ccctcgccct ccaccgcgtt ccacgcgaag
    13741 ccgccgatgc tggtcagttc caggcgcccg ctgggtttaa tcacgcgcac catgccgccg
    13801 agcgtatcca catgcccggt cagcgcgcgg ctgggctctg gctgggcgtc cgcgcctggg
    13861 agcactgcca gcagggcgcc ttttttggtc acgcgcaggc ttaattgcgg gaattccgct
    13921 aacgtctggc gcgtcaactc cactgcccgc gcggcgtagc cggtggggct gggcgtgttc
    13981 aacaggcgca ccagaaagtc ttccaggaaa gcgtagttca gggcaggtaa gctcgggttc
    14041 atgccaaaaa gtcctctctg ttttcaaaag cagccagttt atgccgccat tcggcggaaa
    14101 ctggccgtga atgaccttcg aaatggtcgt acgacaccat caccacatct ccccgcgcga
    14161 aggcccgcgt cccctcgccg tcttccacct gaaagcggaa gcgcaggctt ttggtgccga
    14221 tgctgtccag ggtgaggccc acgcgcacgc tttggtcaaa gcatatggat tccagatagt
    14281 cgcattttac cgagacgacc accatgccca tgttctccac ggtcacgccg tcccagatgc
    14341 cggcggcttg atagtaggcg gtgcgggcgg tttcgacata ctccagcacg gaaatgttgt
    14401 tcacgtggcg gtgcgtgtcc aggtcggcat agcgcacagc gatggggtga tagaaggtga
    14461 attgtttggg ataagcgggg gtgagcgagg tcatattctg tttctcctgt gggaattata
    14521 gtcaatccgc gcgtaaatcg atagttgtga tgcgcagagc ggcggctgga accgcggtcg
    14581 gggtttcccg gcactccctt ccgccggcgc atctggggcg gagaaagtct ctggtccgct
    14641 gcttttggaa gtcggagcgt tggtgtgaag cgttggcaag gggcccggca tcggccccag
    14701 gcgcgggatc gggctgctct atatcgtgct ggggctggct accttcgcgg cgactgccat
    14761 cgcgtacgcg tatacccctc tgcggaacgt ggagaccctg ctgcccgacc gactgccgga
    14821 acaaccagcg gtggaatagc gtagtttttc atttgcgcaa aagcagcctt taaaccaagc
    14881 ctggaccggg aacttttgtc agtaatttga caaaactcag tggatttcaa agttatgtca
    14941 atgttttgac ataacttgct agaaaaaaag ttttgtcaat taattgacaa aactttttcc
    15001 tttgaatgct acgctgtaac tagcgctccc tctaactgag gcctattgtc tttcgccctg
    15061 cgcccacatg cccagccgtt ctttatagcc cacgcgggta ttccctcacc ccatccgccc
    15121 gccttacggg tacaattgaa ccaacatgcc cgaaaatccg cttgacccct ggatagacac
    15181 cgtcatccaa ggcgactgcc tcgagattct gcccaccctg cccgccgaat cggtggacct
    15241 cgttttcgcc gacccaccct acaatctgca gctgcagtcc gagctctggc gcccgaatac
    15301 tacccgcgtg gacgccgttg acgcgaattg ggacaaattc gaaaacctgg aagcctacga
    15361 caactttacg cgtgcctggc tggacgcctg ccggcgcgtg ctcaagccta ccggcacgct
    15421 gtgggtcatc ggttcctatc acaatattta ccgcgtcggg cgcgttttgc aggatttgca
    15481 gtattggatt ctgaatgaca tcgtgtgggt caagagcaac cccatgccca acttccgcgg
    15541 gatgcgcttt accaacgcgc atgaaacgct gctgtgggcg caaaaggaac gcggcgcgcg
    15601 ctacactttc aactatcatg ccatgaaagc cctcaatgac gacctgcaga tgcgctcgga
    15661 ttggtttatc cctatttgca ctggcagcga acgcctgaag gaaaacggtc gtaaagtgca
    15721 cccgacccag aaacccgagg cgctgctcta ccgcatcttg ctggcaagca ccaacccggg
    15781 tgatgtggtg cttgacccat tcttcggcac gggcaccact ggcgcgatgg ctaagaagct
    15841 gcagcggcac tacattggca tcgaggtgga agaatcttat gtgcgcagcg cctgcaaacg
    15901 cctcagccgc ttcacgcagc tggaattcaa cgcccccatt ttcatcacgc ccaacccgcg
    15961 cagtctggag cgcgtgccct ttggcgcgct ggtggaacag ggcttcatcg agccggggca
    16021 ggcgctcttt ttcggcctgg aggacgacct gaaagccact gtgcaggcgg atggttcgct
    16081 ggtctgccgc ggtgagcgtg ggtctatcca cgcgctggcc cgaagcttac gcaaaggccc
    16141 ggcaaacggc tgggagcttt ggtactattg ggacacagag acgcagtgcc gacagccgat
    16201 taacaagctg cgcgccaaat accgcgaaag cgttaaaaaa cattactcag gatgaaagcg
    16261 agaggattgg aaccaaatga tggaacataa agaaacgtat acgcaaagcc gctggagcct
    16321 ggatgacctg ttcacaggct ttgaagacgc caacatcgaa gcgacctacg aaaagctgga
    16381 cgcgatggtg gcggagttcg aaaagtgccg cggggagctg aagcctgata tttccgccga
    16441 acgcttcatg gaaatcgtgg ccagctcaga agcgggtgaa aagctgggct accgcctgta
    16501 cggcttcgcc gagctatcct tcgcggcgaa cacccaggac cagaaagctc agattgccgt
    16561 ggcgcgcatc cagcaattcg tcgccgagat gtccaaccgc acgctcttct tcaacctgtg
    16621 gtggaaagcg ctggacgacg ccaacgcgca gcgccttttg gcagccagcg gcgactaccg
    16681 ctattggctg gagcaaatcc gcaacttcaa gccttacacg ctcagcgaag ccgaagaaaa
    16741 ggtgattaac atcaaaaacg tcaccggttc caacgccctg aacatactct acgactcgct
    16801 caccaaccgc tacgtcttcc acctggaagt ggacggcgag caaaaagagc tgacccgcgg
    16861 cgaattgatg acctacgtgc gcagcggcga cccggagctg cgcgccaagg cctaccagga
    16921 gctttaccgc gtctacggtg aggacgcgcc catcctcgga caaatctacc agaaccttgt
    16981 gcgcgactgg cgcaacgaaa acgtctcctt gcgcggcttc ggcagcccga tttccgtgcg
    17041 caacctgatt aacgacatcc cggatgagat tatcgacacg cttatcgagg ttacccgcaa
    17101 aaacctgggc gtcttccacc gctacttcaa gctgaaagct aaaaaactgg gcgtggaaaa
    17161 actgcgccgc tacgatatct atgcgcctgt caccgcagcc gacaaacgct ttagctacga
    17221 agaagccgtt gcgctcacct tcgaagcctt cgagcgcttc gaccaggcct ttgccagcaa
    17281 agcacggcgc gtctttgacg aaaagcatgt ggacagcgaa gtgcgcaaag gcaaaatggg
    17341 cggagcgttc tgctccacca tcctgcccga attgaccccc tgggtgctgc tcaattacca
    17401 gggcaagtcc gaggacgtct ccaccatggc gcacgaactc gggcacgccg tgcattccat
    17461 gatggcagcg gagcacacgc tttacaccca gcacgcctgc ctgcccctgg cggagactgc
    17521 ctccactttc ggcgaaatga ccctgaccga cctgatgctc gagcgcgaga cggatgcctc
    17581 ggtcaaggaa gatatccttt tcgggcagct ggacgatgct tttgccacca ttttgcgcca
    17641 gatttacttt gccatcttcg agcgcgacgc gcacgagaaa atcatccggg acgcttcagt
    17701 ggacgagctt tcggagcttt acctcgagaa cctgaaagag gaatttggcg acgcggtgga
    17761 cgtgagcgag gagttcaagt acgaatgggt ttccatcccg cacatctacg gcacgccctt
    17821 ctatgtgtac gcctacgcct ttgggcagct gctggtcttc gcgctgtaca aacgctacca
    17881 ggaagagggc gagagcttta agcccaagta tatgcgcatc ctctcggcgg gcggctccgt
    17941 cgcgccggtg cagctgctgg cggaagccgg cctggacgtg accaaggctg agttctggca
    18001 gggcgggtac gatatcattg acggcatggt cagccggctg gaaaacttgt aagctttttc
    18061 ttgatccata acgaatagag cgcccgttag ggcgctcttt gttagcttag atacgtgtct
    18121 acgcagcgtt cttgggggtg ttttccgcct cgagtacgtt ctggatggcg tcgctgatac
    18181 gggtggcggt ctccacgttg ttcatagtat agaggtggat gccgcgaatg tcatggtcaa
    18241 tcaggtccat aatctgctcg gtggcgtaat agatgccggc gtcgaagagc gccttggggt
    18301 tatctccgta gcggctgaag atgcgtgaga gcctggcggg cacgctggcg ccgctcaggg
    18361 ccaccgtacg ctcaatctgg cggatgttgg tgatgggcat gatgccggct tcgatgggca
    18421 catgaatgcc ggcgcgttcc gccatatcgc gaaagatgta aaacttctca ttgtcgaaga
    18481 acagctgggt aatcaggtgc cccacgccgt tatcgatctt gattttcagg ttctcgatgt
    18541 ctttttccaa cgtctcgcac tggtagtggc actcggggta gcaggcgccc agcaggttga
    18601 agctcgggtc gtactgcttg atgaaagccg ccaggtcgct ggcatgccgg aagtcttctt
    18661 tgggcggcac gtccggagaa atgtcgccgc gcaggaccat gatattctgc acctggttgg
    18721 ctttcagctt ttccagcgtc tccagggctt ccgggcggtc caggttcacg caggtcaggt
    18781 gggcgacagg ttccacatgg taggtggaga ggatcaggga ggcaatctcg atggttttct
    18841 cgcgctgagc ttggctgccg ccggcgccgt aggtgacact gataaaatcc gcggggatgc
    18901 cgcgcagcct gagcagcgtg ttgtaaatcg tgttgtacga ggtgtctttt ttcggcggga
    18961 agatttccag cgagaagacg gtctttttgt ggtcgaatag tttcgtgata ttcataagcc
    19021 tctccaggtt agagcattat tttatcctga gtgcgctgga atgggaaacg gcttgttccg
    19081 ggctgccaga acggtagata atcttttgtt atccttcaga attgcattat atttttaaca
    19141 tattcattga aaggaaggaa aatccgtcat ggaaatcgat gttgagactg aacgtgtgcc
    19201 tagaagcaga gaggtgcatg tgcatgaatc cagtgcattt tacgggttgg gttttatcgg
    19261 cgcggcgatt tattttatca gcacggccac cacgttctgg atgggcgtac tgggctttct
    19321 gaaagcgttc gtctggccgg ctttcctggt ctacgaagcg ctgaaggtgc tgtactagaa
    19381 cacgcgctcg cttgtcaccc tggcgcggct gcccggttcg ttagcatgag cacggcggct
    19441 tgcttgtcac gctgagcgaa gtcgaagggt cttgtaggga tttgtttcgt tcgtcggtgc
    19501 ggacctggcc gttcgtatcg cgagcttaag aagcgatctt cccctttttt ccttcgtcaa
    19561 cccgatcgca ctcccctaac cgtctctgcg cgaccctttg ccaatgcctg acctctgcgg
    19621 ggaataaggc agctggcaaa gggtcacggc aatgcgaagc tcccatggtg gtgcctgcct
    19681 gtcaatccct gacaaatttt caggtgatgt gaaaaaaagc cgccaaatag ttttcaaata
    19741 tccctatgca atctggcaga attttacata ctaaaggcag attgccgcac cgttttgcct
    19801 ggcaacctgg gtcgcttggc agatcattgg ttggcaaaac ggttcgcaat gacggatctt
    19861 cgtcgcaagg acgaattgcg cgtcagcgcg agcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19981 nnnnnnnnnn nnngtcgcaa ccggaaggtc gtaggtcggg tgcagcggca ggacgttcgt
    20041 aggtcgggtg gagcgccagc gcaacccgac aagctttggc cagcgagtgt ggagccgttg
    20101 gcttccgccc ccgcgcctct tcacccggcg taaacggctt tcatcctgaa cgcagcgaag
    20161 aatctatctt tgaaccccct ttcggcggtt cgaaaaacgc aaaccgccct acaaccgacc
    20221 ctccttcgca atgacaaatc gttccccggc agcaatcccg ggcatccctt caacacgtgt
    20281 caatctacgc ccgataagag cttatggtat attttacgca tcacttctcg ctcaggagca
    20341 gctgttcatg ttcattgcca tcgaaggtgt catcggcgtc ggcaaaacca cgcttgcccg
    20401 tttcgtccat tctgctttct ccgccgacct gctcctggaa gtcttcgagg aaaacccctt
    20461 cctgagcgat ttctacagcg accgcagccg ctacgccttc caaacccaaa tcttctttct
    20521 gctcagccgc tatcaacagc agcaccgcgt catcccggat accctggcag ccggacgaat
    20581 gattatctcc gactacacct tcgaaaaaga cgcgctcttt gccggcatca acctggccgg
    20641 cgatgagctg gcaatgtacc accgcgtgca tgcagccctg gcggaaaaaa tcccacagcc
    20701 ggacctggtg gtgtacttgc gcgccagcac tgagacgctc atgcagcgca tcgcccaccg
    20761 cgaccgcccc tacgagcgcg ggatggaccg ggcatacatc gacgagctga accaggctta
    20821 cgaggcgttc ttcgcgcacc cccgttggga aggccgcctg ctgccgattg aaacggacgc
    20881 cctgaatatc ttcgcccgcc cggacgacct gaattatgtc atcaaccgca tccggcagac
    20941 gctgcgcatt gcgccctatc aggaaagcct gccattggaa ggctgaagga ggaggtatcg
    21001 tgcatatcac ccgggattta ctggtcaagt ttgccaggga atatgtcgca aagaaaacgc
    21061 gccagtcgaa cgacatcgtc gccgtgtacc tcaccggttc cgtcctgacg gacgacccat
    21121 tgattggcgg cagcacggat atcgacctgg tggtcgtgca caaagagaat ccgctcttcg
    21181 aacgggaagt gcagcgcatc agctacgaaa tctcgctgga tgtgcagcat caccaccaat
    21241 ccttctacac ttttcaccgc cgcttgcgcc tgaacccctg gctgggcttt gccttgtgcg
    21301 atcacaggtc tatcctgtac gacaccgagc actggctgga atttatccag gccggcgtca
    21361 gcgccggctt caacagcccc gagactgtgt acgcgcgcgg gcaggccgcg gcggataaag
    21421 cccgccagct ttggtttgaa ctggaagatc cgcaggaact gccttttgcc ttgtggaccg
    21481 acctgtacta taaggcggtc ggcagcgccg ccaacgcaat cgcgattatc aacggtccgg
    21541 cgcttaccac ccggcggatg cttttggatt ttcccgcgcg cgcggaggcg cttggtaagc
    21601 tggaactgac cggcgaactg ctgcagatca tcagccgcgc cccgattaac actgaaattt
    21661 tgaacacctg gcgcccggtg tgggaaaacg cgctgagcgc cgcgagcaag ctgcccgact
    21721 gcccgcctaa tctgctgccc gcccgcaaag cctactttgt gaacagctgc gctgccatgc
    21781 tggaaagcgg aagcgcccac gccgcgctct ggccgatggt ggagacctgg cggcaggcga
    21841 atctgttgtt atccgaggat cctgcccaac aggaagcctg gcaaaagttc ctggaagcgc
    21901 tggaaattac cccgttcgac aaggatgaac aaatcgaacg cctggacctc ttcctggaca
    21961 atgccgaagc agctttggct gagatgaaag cggagtatgg gctctgaatt ctaaattttc
    22021 cccttttttc cactttttct gtggataagt gccggaaatc tgtggataag tctgcaaata
    22081 tgtgaataac tcgagcaaat attctatgtt caacgggaaa acctgttgaa aactcaggtc
    22141 ctttaaagcc cgcgagaaga acttatccac aagtttttgc tttttccaca aaaaatccaa
    22201 caccactgca caaatctgcg gcgcacaaaa acaggaaagc accgccacat attctgcctg
    22261 cacaagagca agcccctaca tagccggcct tgaaccgctt ttcacagtct ctatccacat
    22321 ctccacagtc cctactgctc ttactacctt aagaattatg aaatgaatat agatctgtgt
    22381 ataaaaaaac ggcagcccaa ccgaactgcc gtaaaaaaga agtacacaat cttgttaaag
    22441 gttgacttcc gttttaccca tcagcctctc ctgcaccagc tgcacgacct ggctgatatg
    22501 cgcgctgctg gaaaccagat tgaggttatc gctcgggata atcagcaccg ggcactgggc
    22561 gaaagacgcg atccactctt cgtacaagac gttcaaacgc tccagatagc tgcgcgaaat
    22621 gccggcttca aagccgcgcc cgcgcgtgtt gatgcgctgc aatagcgtat ccaccgaggt
    22681 gcgtaaatag accaccagat taggcggagg cagcaggtca atcagaatgc ggtaaaggtc
    22741 ctggtaaacg ccgtagtcgc gcgtactcat atcctcctgc aggtacaggt tgcgggcaaa
    22801 aacttccgcg tcctcgtaga ctgagcgatc ctgcaccacc gagccttcgg catccagcag
    22861 ctgcttatgg atgcgcaggc ggcgggtgag gaagtaaagt tgcgaatgga aggaccaggc
    22921 ttccatattg cggtaaaagt cttccaaata ggggttgtcg tttacaggct caaaaaaagg
    22981 cttgaagccg agccgttcgc tcagaatctg aaccaaagtg gacttgcccg cgccgatatt
    23041 gcctgccaca acgatgaact tatccatgct gccagctttc cgaatcagat gagttagctg
    23101 tagtataaac gcaatctggg ggaaaatcag gacaaaacca gggcttttcg ttcaggattc
    23161 gacagctgcg ccttggggac ttacgcgaat gcccggcacc aacagaacag ccccggcgtt
    23221 cagccccgcg gcggccgcgc agaccagcca gaagctgttc tgatacaaac cggggctgaa
    23281 cagaggcccc agcagcctgc caatcgagag tgctgccatc aaaatcgcca gtaccgccgc
    23341 acgcgaattt ggcagcacct cgctgaccgt ggtcatcaga cttatcagcg cgaattcaaa
    23401 actgacataa aagagagcca acccgaccag cgccaggcta aaggaagttc tggtgagggg
    23461 aagggccagc gccacgagcc cgtttattat caacgcaagc cgcatggcgc ggtgtttgcc
    23521 aattttatcg agcaccgcgg ccgagagcag ttccgaaccc agttccgaag tgccaataat
    23581 taccgaagca actgccagcg cggcaaagtc cagcccgaaa gtatccttaa tccacacgct
    23641 gaaaatgagg ttaatcatct cattcgcgcc cgtagagaag attccgacca gcgcggcggc
    23701 cagcaccgga aaatagcgca gcgctttgcc caggttcgcc aggacgcttg ttccttggtt
    23761 ggcaggcgcg cgctgcgcag gcaccagcag ccacagcgcg gcggtcaaaa gcacgccggc
    23821 gatggttagg gcgctgaaag gcgcgaccca gccgccagtg ttcagcagcg cccccagcac
    23881 aggcacccca acgatgaagc ccagcgacca actcagctcg gtgatggaca gcaccgtccc
    23941 tctgcgctca taagggactt tttcccccag gtaggcttgc atagagggga tgaagatgcc
    24001 gttgccgacg ccaaagactg acagcccgat gataaagctc agaaagttct gtccaagtgc
    24061 ggtaatgccg cagccgacaa aaaagagaat gacgccaagc atcaaaccgt ttttgcgtcc
    24121 gcgcgcatcc gccatggaag ccagaaaagg attgaagata cccagcacgg aacggatgga
    24181 aaacgcgatg ctgaggtctg ccagctgcac gcccatgccg gcggcaaaga gcggaagcag
    24241 cgggtacacc atacggtagc cggagttgat gaccgtgcgg gtgagcgtaa acaggaaaat
    24301 ttcagccttg gtacgcgggg aaatagggtt gggcatgcag cgggaacctt gggcgcgtag
    24361 agctttgctt aggccggggt ggcaggctgg tccagcatat cctgcagggt ggtgcccttg
    24421 agctgcgcga taatctggtc ttccgtctct ttccagaatt tcagataggg gcaggcgtcc
    24481 gcgagcgggc aggtctccgg gtcttccgtg cagtggatca ggttgatctt gccgtcgatg
    24541 gtggtcacaa tgtcgtagat ggagatttca gccgggcttt ttgccagcat gatgccgccc
    24601 cgcgcgccgc ggcgggtctt gatcaaaccc gctcccacca gttcagaatt gatccgggaa
    24661 agaaaaatgc gggagatttg catctcttcc gcaatgacat tagagggtgt cagacggttc
    24721 tgtccattcc gggcaatata aatcatagag cgcagcgcgt aatccgcctg attggtaatt
    24781 tgcattgcca tagtatatcc gtttccaact ggtcgcctgg aaaaccaggt gtggtaagtc
    24841 cgttctgcaa acggactgaa ggaagggggc aaataattac tatgcctatg atttatttta
    24901 ccgcaagtat gcgtaatgca tgaccatccg ttgcggcttc cacccaggtc cactctgaaa
    24961 gtgcgatcac ccggctgatt tccgggtttt gttcaaggtc tgtcaagagg cgcggaccga
    25021 ctgccagacg cgcctgcgca tcgggcattt cctgcgcgaa cgcgccaagc cacaggctga
    25081 ctggctcgag attgacagca gattcgaatt ggacaagcgc tgaaaaatcg gcgctgcgca
    25141 aactcagcgc tttgagcgcg tcttccccga gcgtgaatcc gagcacagcg ctgccgccca
    25201 agagcagctc gtcttgcggc tgcagcctgc gctggaggac gcccgcgctc tggaaagttt
    25261 catacaggcg ccgcgtggtt tgcagccgct ccgggttgca agcccagtag accgcgccga
    25321 ttcggaaact ttcgctcaag ccaaacaacc cttgcacgtc atctttgcca caagctggaa
    25381 tgatcagggc gtccagttcg cgctggaagg gcggcagcgc gctgcccagc tgctgcttaa
    25441 gcgaggcgga gggcaggctc cctcccacta gcaggtagcg cccttccgcg ccgcgcacaa
    25501 gcagcaccgg ctgctccggg ctgttgaagg cgcgcaagtg cagctgcccg tcaggcgcgc
    25561 gggctgccgt gctccagacc agcacaacca ggctggcgcc gataatcatg agtgggatgg
    25621 ggcgggccag agctttggca ggtttgtgtt tcagcgggac tgccaggctg agcgccaatg
    25681 cccaggcagc cagcacccaa aagaagctga acggcggaaa gcgccaggca gtcgggagca
    25741 agcccgccaa ccaaagcacc gcgcggttgg tgtacgcagc gggcagccag gcaagccagg
    25801 caagcgcctt tccaaggcct aaggagagca agccccctaa aagcgccagc atgcccaaca
    25861 gcatgaccag cggctgggcg ggcagcacca gtgggttggc gagcagaaag agaggcgaga
    25921 tttcctgaaa gtggtaaagg acgaggggca gcgtaagggc ttgcgccgcg aaagtggtca
    25981 gcaggtattc gccggcggtg tcgctcagac cccgggcggc agcctgtccc acgcgtgcct
    26041 ccagccagcg ccgcaggcgg gcttgcatgg gcgcgctgaa cagcagaatt ccgacagtag
    26101 ccgctgccga gagctggaag ccgatatccc agggcaggtg cgggttgaag agcagcatca
    26161 ggcagacgac caatcccagg ctgttcagcc cgctgccgcg ccggttgatg gatgcgccca
    26221 taattcccaa tgaactcatc agcgccgcgc gcaccacaga agcgtctgcc ccgaccaaaa
    26281 cggtgtaaaa cgccagagtc aatatcgcca gcccgccgcc gcgccaggct cccagcttac
    26341 gggtgaagag cccggtgatt aaccccgcca gcacggccat attaaaacct gagatggcga
    26401 tgatgtgcgc ggtgcccgtc agggagtagg cgttttgcag ctcggcggag atgccgctgc
    26461 gctcgccaag cagaataccg cgcagcaggg cgccctccgg ctccgggaaa atccggcgcg
    26521 agaccaggag cgagcgttcc cgcaggcggt agatggcagt gtagaaaggg ctgccctgct
    26581 cgcgctgcag cagttccacg cgcgcgtact ggctgagcgc gctgatgccc tgatggaaaa
    26641 gatacgcttt ataggaaaac gtgccgctct cccccgcctc cgtgagctcc ccacggatgg
    26701 agagcaggtc gccgtaggcg taggcgctgc ccggcattgt ccgcagcagg atttttccgc
    26761 gcactttctc cggccgcact gcttccacgt ccgcggaaag ggcgtttacg gcttgcacct
    26821 ctacgaccaa ttcctgcccg ttctcccaag cctcgggcgt ttcgctcacc acgcccaaga
    26881 cctcgacttc gcctttgccg acgtagtagc caatgaatgc ggcggtatcc tttggcaaag
    26941 aaagctgata gagcagcgct gccaggcaaa aaccagccgc aagcagggat aacggcaatt
    27001 gtttgcgcgg ctgccagcgg aatctgcgtt ttgcatgccc gagcgcgccc aaaagacaca
    27061 gccccaacat gcccgcccac cactgccagg gcagcctgag ccagcttgcc cccacagcgc
    27121 cgcaaagccc cgcgcaggcc agccagaaaa agggcattac cgcggggata tgcacctgtg
    27181 tcgggtcggg cgtgggcggc ggggcgggcg cggcggtgtt cacggcgggt ctggtctccg
    27241 ggcctaaggc ttgctgcccg gcgagctctc cgtgctgatg aattcgagca agagctcaag
    27301 cgctttttgc gcgagcattt ctttgttggc ggcccggtct ccggatagca aaaaatgccg
    27361 ggaccattcc ttgccggctg cggctaagcc cacccaactg gtgccgaccg gcttgtttgg
    27421 ggtagcccca cccggcccgg cgatgcagga cacggaaagg gcaaaatcgg tcttgaacag
    27481 ttctctcgcg ccgcgcgcca tttccaaaac agtctcgctg ctgaccgccc catagcgctg
    27541 cagcgtctcc cagcgcacgc ccaaaagcgc cactttggcg gcataggcat aagcgaccac
    27601 cccgccaatg tagtattccg aggagcccgg aacattggta atgcggtggc tgatcagccc
    27661 gccggtgcag ctttccgcca gcgccagcgt ccatttatgc ccgccaagga ttttgccaat
    27721 acgcgcttcc agaccgggcg ggtccccttc ctggcgctcc tcaacaggca ttcttccgga
    27781 agaatccagc tctctccagc tttccgccag gtgattgctg cggatgtact cggcggcagc
    27841 cgggctgagc cagccttttt gcaccaggcg ctcgggttcg gcgccgctct ccaactcccg
    27901 gcggatttct gaggaagaaa tctccggcgc gcttacctcg tcgagcagga tgatttgcgc
    27961 gcctttcgct tccagctcgc cgatgaactt caggttcggc gcgaagccct gccgcgggca
    28021 caccaccagc gcggtcagcc ccaagagcgc ctccaggtga aaccagtggt tgagggaagg
    28081 gtcctctccg gaaaagctgt cggttccgag caggaaagcc agacgcagct ttgggtcgca
    28141 ttcttcgcgc aggcgggtaa aagtttccgc gctcaggttt gggcggtggc tttcgcgcgc
    28201 gatttcctct tccagcctgc tgacagaaac gcgctcaacc gtgccagcca gatcccgggc
    28261 gatatcgccg gcagccagct ggcaaagctc aaaacggtgc tcgtaagtga acggcaggga
    28321 acgtttgacg gcatccaggg tattgtggtg ccggtaaacc ggcataatca tcacctcgcg
    28381 caccggccag ccgcgcccgc tcagcgcccg caccgcggct gtaatcaatg cgctgtgccc
    28441 tttatggaaa gggtccgccg aactgccaaa cacaagcaaa aatggctgga cgttgctcat
    28501 gggttttctc ccggattcgt atccaaattt tccagctcat taagccagag cgcgccgctg
    28561 gcgtccgccg gcatcaccca gccgccgcgc gcggaaaggg agagcgcgct gaccttaggt
    28621 ccttccgggc cggccgagcg tttgaattgc tgcctgaaaa atcgccggta aaacaggcgc
    28681 agccacgcga ggacctgctg cggggcgtac tgcccggcaa aggcgctgag cgcgagctga
    28741 tagacctgcc gcggggaaag cttgtttacc aggctgtgat acaggaaaaa gtcgtgcagc
    28801 aggtaaggcc ccagcaccgt ttccgtttcc tgcgggctgc tgccgtccgc gcggaccggc
    28861 agcagttcgg gtgaaatcgc tgccgcgcat atgctggaca cagctgctgc gccgtcctcc
    28921 ccgaaaagct cacgccccgc ccaagccaac acgcgcagga caagcgtttt gggcaggccg
    28981 gtgttcacgt ggtaattgga ggtctggtcg cccgcaaagg tgcaccagcc caacgcgatt
    29041 tccgaaaggt cgccggtgcc gaccatcagc gcgctgtgtt ggttggcaag gtcgagcaga
    29101 atctgcgtgc gttcgcgcgc ctgcgcgttt tcgtatgtaa ggtcaaaaca gtccggtggg
    29161 tgcccgatat cccgcaagtg gctctcaagc gcgcccgtaa tcgggatttc gcgcagcgtc
    29221 acgccgcaca agcttgccag cctttccagg cgttcgcggg aggattgggt agagcccggt
    29281 ccaggcaagc tcagcgccag gatatcctgt ttctccaggc ccagcagctg gaatgcctga
    29341 aagctgacca tcagcgccag gctggaatcc gcgccgccgg acaggcccaa cacgaccttg
    29401 cgcagcccgg tacggcgcag tctgccggcc agaccggcgc tttgaatggc gaacgcggcc
    29461 gcgcactggg cgtcaggttg gctgacggag aggaaggggg tctgagtgag cgctggtggg
    29521 ggcgcctcgt tactttgttc ggctgctttg cgcgcggagc gtttgcgggg gccaatcagg
    29581 gcgcgggcgg attgggtaga gaactgcagc ggctgcgttt cggccagggt ctcgccgtcg
    29641 tgcaggatgc cggctttgcc gctgaacacc gcttccgccg tggattcgca cggcccgcaa
    29701 gaggcgtacg cccataccgc ctgccgtccg cgcaaggaca ggcgggtctc cggggtttgg
    29761 tcggccagcg ccagggcggg cagggcgcag ggattgagca acagccctac gcgcatttcg
    29821 agtatcaggt tttcagtccg cccgacgcac agttgaattc tttccgggta gagttccggg
    29881 agccgcaagc tcaggtccgc ggcggccggt acgctctgcc ccagaatttc cgcctgcgtg
    29941 tcggggaatt cgcctcccgc gctgaaccag cggctcggga acccggacag cgggtcgaga
    30001 ggttctcttt ccagcgaaag cccggccggg ccgtgtggac ccagcagcac ggcggcgttg
    30061 tacaggcgtt ttacgtgctg gaacgggagc ccgaccaacg cccaggtttc catgctgcgg
    30121 cagcctgcgg caatgcgctc cagggctctc aaggcgcttt ccaccagcag gggctgcagg
    30181 aacaaatcgc cgcagctgca gccggtcagg cagagctcgg ggaaaactgc caggcgcggc
    30241 gcggggttct gcgcggttgt ttgctccagc gcgcgcagga tttcccggca gttaaagtca
    30301 acgtcggcag cacgcagctc gggcacaacc accgagagct gcaggccgcg gcagggagtt
    30361 ttggtttggg gtgcaggtga gcttcccaca gcaaagatcc ttgaacagct taatgataac
    30421 aagcttttcc gggctgtgcg cgatggattg atagaaaatt tcgggaaccg gaattttctc
    30481 cggcatgttc aggcctggct ggcaaattcc tgcacgcgct caagctgctg cgccaccgcc
    30541 agcgagtctt cccggaagta ttccagggtc agccattcgg gctgcacacg ctccagggtc
    30601 cattccagca ggcggtaatc ttcgtccgtc agcgtttcat gcgcgtcgaa aaggcgcccg
    30661 tcccgcccgg cgcgcactcc tgccaggtgg atctgcacgc ttttttccag cggcagcgcg
    30721 gaaaggtagg cttcggggct catcctgcgc gcctgcgcgg caatgcgcgc gtgcgccagg
    30781 tccagcagca gcttgcagcc agtttcttcc aacactttat tgattacgga tggttcgctt
    30841 tcgaacaggt agcggtttgg gtgcagagcg ggcatgttct ccaggatgac gggcagctcg
    30901 gaattacgct gcagccactt cacagccctg ataaagctgc gtacggataa gcctgccagc
    30961 ggaccaggca gcagcctgcc gcggtgcata tatgcgctgg tccacagcca aggcagcggc
    31021 gccaggtgca cagaaacatg cggcgactgc gggcaggcct gcaggtaagc ccgcaaatgc
    31081 gccagccagc tgtgtgtgtg gtggaagcgc cccgggtgaa aatgaaacgc cacctgcggg
    31141 aactgggcgc ggctctcggc tatctggctg agcgggagtt tatccaccca ttccaccgcg
    31201 tccacgcgcg cggcgcctgc gctcatgaga ttgagcagcg cctgggaata gttgagggat
    31261 actttcatgg gcaggtctca gtctcgttcc gggtgagctg ctggtgaatg acgcgcgaaa
    31321 gccgggcaaa ttccgcgctg tagcgcatct cctgccggcg cggtctgggc agcggtacgg
    31381 gcaggtccag gctgattttt gccggccggc tggtgagcac cagtacgcgg tccgaaaggt
    31441 aaagcgcttc ggagatggag tgcgtgacca tcacgatagt tttgctcttg ctgccccaaa
    31501 tgcgcaggag ttcttcgccc atgcgctcgc gggtgagcgc gtccaactgc ccaaaaggct
    31561 cgtccagcag cagaatttcg gggtcctgca ccaaagagcg cgcgatggcc acgcgctgcg
    31621 ccatgccgcc ggaaagttcg tgcgggaagt ttttctcaaa gccgcgcagc cctaccagct
    31681 cgatcatatc ccgggttatt tgctggattt ctgtcggact tttgcctgcc atctcgagcg
    31741 ggaggctgat gttgttgaac agattgcgcc agggcatcag gttgggcttc tggaacacca
    31801 tgccggtcag gctgccgccg cgcgattgca gcggaaagag gatttgcccg gaggtaggcc
    31861 ggagcaagcc gccaatcgcg cgcaggagcg tgcttttccc gccgccagag ggcccaatca
    31921 ggcacacgaa ggaattctct tccacccgga aagacaaatc gtccagcacg tccaggctgc
    31981 cgtcgctttc cacaaagctg atggtcaacc cgcgcacatc caggacggtt tcaggggagg
    32041 aagctgccgt catatacgcc tttaaggtcc accgcctggc tcatcaagcc gagcttcaac
    32101 aggacggact gcatattctc ccagcgccgg gtatggtcct cgttagtggg atagacgtcc
    32161 cacagcttga tggactccgc cagcacctgt ttctggacgg cttcgtctgc ctccgccagg
    32221 ttgtccacgt atttcgtgca gatcaagtag gtctcgtccg ggttttctgc gctgaactga
    32281 atgcctttga gcagcgcgcc gagcaccgcg cgcaccagct cggggtcctc ttttatggta
    32341 ctttcgttgg tgaccaggcc gttgccaatc aggtcaatgc tgtcagccac gcgcaaggtg
    32401 ttgatttggt agcccatggc gcgcagttgg gagggttcgt tggaaaggta aatcaccgcg
    32461 gcgtccacta tcccgctcac cagcgattcc acctgggtat agccaatgga ctgcagggtg
    32521 taatcactgt cgcgcaagcc ttcgttctgc gcgaatgctt cgaagccgat atagttcgcc
    32581 ccgtataagc ccggcagacc aatcgtctta cccttcaggt ccatcgggga ggtgatattc
    32641 ttctccgcca gcgagacgat gccgaccgga aattccttgt accagttgta aaccgtcacc
    32701 acaggcagcc cctgggcgcg ggccatgagc acttgttctc ccgaggcaat cataaattgc
    32761 tgcttgccgg agccgaccaa cgcgataagg tcggcttcat tgccgtagtt aagcgttacg
    32821 tccaggcccg cgtccgcgaa gtagccattt tcgatggcgg cgtagaacgg agcaaactgc
    32881 acattaggga tgtaggtcag attcagggtc acagcgcgca aaggcccgtt ttgggcgggc
    32941 ttggcgcctg tgcaggcagt cagcagcagc acagtcagca taaaaatcgg gttcagacgt
    33001 ttcatcataa ggctctccaa gtctacgcgt tcttccgcca ggtctgccag gccagcaggc
    33061 gcgattccag caggaggatt aaaccataaa gtgaggctgc cagcaccatt aaggtgagca
    33121 cagccacaaa aaccagggca gtatcgtact gacctctgcc cacgttgatc aggaaaccca
    33181 gtccgcggtc cgaaccgacc aattcgccga caatcgcgcc gatgaccgaa agcgtagccc
    33241 ccacgcgcaa gccgcccaaa aagacaggca gactggcggg cagttccagg tagcgggcgg
    33301 tctgcccggg ggaagccttt aaggaacgca tcagctcgcg caggtcctgc ggaacttccc
    33361 gcagggccac caaggtattc accagcaccg ggaaaaagac agtcagcgcg cagatgagca
    33421 ccttggagaa caagcccggg ccgaaccaga tgaccagcag aggcgcgatg gccacgacag
    33481 gcaccgattg gctggcgacc aggtacggtg aagtgaaacg ttccgcgcgg ggatgcttga
    33541 acaggaagta cccgaccagc gcggcgctca ggctgccgat taacaaacct gagaggactt
    33601 ccaggagggt gtaggagaga tgacgccaga gcagcccatt cccaagcacc tgcaagaact
    33661 tttgccagac gaccgcaggc gccggaagga tgaagccggg cagcttcagc agcatccaga
    33721 gcaactgcca gacaataacg ccggccgccg cggcgagcaa gaccgtcagc gcgcggctgc
    33781 cgcccgggcg cgtgtagccg cgctgtttga tgtcagtcag gtttttcatg gtgaattatg
    33841 gtacaaagta taaaaaaata aacggcttaa gtcaattgat tcgtttgcag cgatcggcgg
    33901 agtttgcgat aatggcaacc tgtcttgatg acaccctgaa aggtcaggcg ccgcatccgg
    33961 acacctgcaa gggcctgcgc gccgctcagg gataatcatt tgcagcagcg cgctaacggc
    34021 cggctggcag atcaacgggt acgcggagaa cacctggcgt ctcgcgatga ggcgcgattg
    34081 gtcattcggg agagctcggt gaaggtcggg ggtttgcggc tgtcagcttg cttcttccat
    34141 aagaatgccc acggcagggc acacccttcc aataaagact gcgctaagcc aaaaatagaa
    34201 aaagtcttgc ttatcgggat attttcctat tcaaaaaatg tctgggcgca gcttgtttca
    34261 cgttacaggc agcagatgct cagaaagctg gaggaggaaa ggcagcctgt ttgggtctgt
    34321 ggggaatcaa cccgcacaaa acgctgccgt ccgggagcaa agccccgatt aaggcataca
    34381 atccggcatc gtctcaggat aaagcgtaaa cccgaaggtg tttaccttat cgctaagata
    34441 ggaaatgttc tcaagaatct cataatttgc gccgtaaacg ccgttaaaga taacgcggaa
    34501 gtaattcaca aggctgatat ccggcgtgat ttgtgccttg acggtatccg gcacatagaa
    34561 agcgctcagg accgccaggc ggttggcgct gtcagcgccg tgatcgccgg ttatcacgat
    34621 aattggcggc gaatccgagt tcttgagaat cgcttccaga accggaagga tggcgttatc
    34681 ggtatactga acaccccagg aatagtactg caagtattgg tcccagggga gaggaaccac
    34741 ccacgggagc tcgggagggt caggaatgag cgaaccatcc gggtagaaca cgtaaggcgc
    34801 gtgcggcatg gtcagatgag cccacaccag tttgggtccc tggaaaccag aaatctgctt
    34861 cagctccttc aggatggtcc attgttggcg cacatggtcg gcataaggat aattgagcgg
    34921 gattttgtcg gatgttgtga ctccgccttt gtaatcagta agaattcgga ccagggtagt
    34981 gtcgaagagc atccgctcga atttactgaa cacaacccgt tcttcagtcg aaaccaggct
    35041 ggcaggatcc aacgggtagt aatggtctgc gtctgtccag gctatgcgct gataagaagc
    35101 cagtgagtag gtgctgtacc ccagggattc caggttcttt cggactgcgt tatgctcgat
    35161 taactcaaac agcgaatcgg tgtcgttatt tggcggcgcg aaatccgggc tgatgtcctg
    35221 tagatactgc atgttaagaa acgaggacat ggagagcgga gtataagcgt aattgctcat
    35281 actgcatggg ggcacgtaga agcccatctc ggtaagttga ttaataaagt tggtgttatc
    35341 cagatgaaag gtcgcatcga gcacgtcact gcgcaggtga gcatccagga taatgatata
    35401 aacatccggc aaggcgcccg gctggagctt cacatcttcc gccgcggccg caaccccacc
    35461 ctgggatatc ccttttccgg cgccggtcgc gcgtatttca taggttatca gttgatacac
    35521 cgggataatc aacaaaaaag ctgaaaaaac attcagaatg atgctgagat tgccgctgtc
    35581 tttcacatat ttcaagagca gaactgcacc aacgccagcc aaaactgccc ataacggcag
    35641 caggatgcgg tgatgaccaa aaacctgaaa gaggctgccc gctgcttgcg cagcgctgta
    35701 ggcgcgccca taggtgtaaa acagcaggaa cagccaggcg ctgaccaacc cggctttttg
    35761 aatagacctg gtcgccagca agagcgccag ccaaacacac accgctccgc cgagtatcat
    35821 cagcagcggg cgcaggccgg cgcgcaggtt aacctcttga atgttgtgac ctaataatga
    35881 gagtaccgga aacgccgcga aaaggagcgg ataaaaagga aagggcagtt tcggcttcgt
    35941 gttcatcttt gctatactgg cgtgatttta ccactttcgc cgggctgccc gtctttcaga
    36001 atttgcgcgc gagcatgggt ttccaaatga gaatgcaatg aaaaggcctt ggcggaagaa
    36061 cggactgcca gttgtcaggc cggcgatttc aattgcgagg atagaaggat attttgagcc
    36121 cccgcgcggg cttacaggcg gacgcggtga atctcaaagc gatactcttc cgcatgccca
    36181 tgggaattgc catgtccggc gccaaacccg ctgccggaat gccggaattt agagcgaggg
    36241 tctcagccga aattcaggag agcttctgct tacgttccat gaggaataaa atccgctcgg
    36301 acccggcaat cggggcttcc tccaaaattt caaagtgcgc cccgaaggcc tttttaaagc
    36361 cggcttcagt gtagtccgga aaaatgtccc gccgggagga cagcagccgc ttcacctggg
    36421 aatctgactt tggaatgaac tcgacaatca gccaatgccc aagcccggca aaaaaagctg
    36481 ccaggttctc cagaggaata ttgtttgata tcgccagatg gtggaccaat gccagggcca
    36541 tcaccatatc gaccgggccg cgttccagca aagacattct ttccgcgcaa ttccagccca
    36601 gggctggcga aggattggtc aggtccatgc aaaggggcag cagctgcgct tcctggtttt
    36661 gtttcacaag ggcgtaattc cgctgaaccg ccgcgtggtc aatgtccaac gcgatggtcg
    36721 ggattcccgc gctgctggct gcccggctga attcaccggt attggcgccc aaatcccaga
    36781 cagaacgagg gcttaccctg aggatgaacg cgctcacttt ttcgcgtttt gcctcgaagg
    36841 atgcccgcgc gtaattggtt tgttcgtagt aattctccca ttcggtcttt tgcgccttca
    36901 gcttcaggct gcggaccgcg gaaaggaggc tgtccagcaa tcccaagagg gcgtttcttg
    36961 tcattttgcc cgagacttgc tcttccggca gcgccatgtc ggcgtatttt tgctgggtct
    37021 tcgcgtgcag gtggatatgg ctgagcaaac ccgggtttag caggctcccc ttggggagga
    37081 gggaactggt caggtcaagg ggaataccgt caatgtaaac ccgcagcagt tggttaaggc
    37141 ggatatccac ttttgccatt aaagtcagcg gcgccaggaa atgctggcag aactgcttgt
    37201 acgcaatcca gggagcgccg ggcagataac gttcgaagga aagcgtatcg atgaaaatcg
    37261 gtcggccgtt tttgaactga acattaaacg cgctggcgtc tttcaaaatc atcccgtact
    37321 gcaaagcgcg tctggcaatg gaaagcgtca gcactgccgc gtctttcaat tggtcgaagc
    37381 accattcata aggataggac acaaaaggga ttggctcagg tttaaggact ttgtaggcaa
    37441 gcccgggaac cggcgcaatg tccaacgagc attcttcatg cgatatgagc gcccgggcgg
    37501 agcttaagtg ctggtacagc cccgattcca tcagcaggtc ataatcttcc tgatagctct
    37561 ggttcacctg acggtagaga acgccgtcct ggtggaacat aaaaccgctc gggtcacgga
    37621 atgaactggt caacttcatc gttttgcttc ccacaaatca aggaatagcc taaatcaaga
    37681 gaatgctgtg agccgttact tcaagctgtc gtccgggttg ttcggggtgg attccggctc
    37741 gacaatttcg gttgggtcga ggtcagcccg taaagcgttt ctgttcggat tgaaccagct
    37801 gcgtattttt ctccagaata ccttaacaag gacgccaacg ctcagtaagg ccgccagaag
    37861 aacctggagg accatactgc cggtgcccgg gtccaggtaa tgggtcagtt tttcaatggg
    37921 cgcaaagaaa atattcattg cttgttcctt cggggtttat tttggagctc attataatct
    37981 gaatcctgag ccgcgctccg aaggacaggc ttctcatttg tgcgcgaagc aagtcagcct
    38041 gcctgcctgc agtataatca gcttaactca ccttaccaaa gcagaggagc gcatgaccga
    38101 taaattagcc tggattgatg acgaactgaa gaccctcaaa gacaccggtt tctataacag
    38161 catccgcacg ctggactccc cgcagggcgc ctggctggtg gtcgatggga aaaaagtgtt
    38221 gaatttctgc tcgaacaact accttgggct ggcaaaccac ccccgcatcg tgcaagcagc
    38281 ctccgaagcg atgcaaaagt acggcgtcgg ccccggcgcg gtgcgcagca tcgccggcac
    38341 catgtccttg catctgcagt tggaaaagcg catggctgcc ttcaaaaaag tggaagccgc
    38401 catcactttc caatccggct ttatggcgaa cggtgccacc atccccgccc tggtcgggaa
    38461 ggaagacgtc attttctcgg acgctctcaa ccacgccagt atcattgacg gctcgcgcct
    38521 ttccggcgca aaaatcatcc gcttcgcgca cgcagacccg gcggacttcg aaaagcaaat
    38581 ccaggccgcg gccggcacgt atcggcgggc gctgatgatt agcgacggcg tgttctccat
    38641 ggacggcgac cttgccccat tggataagct ggtggaaatc gccgaacgct atgacgtaat
    38701 gaccatggtg gacgacgcgc acggcgaagg cgtggtgggc gaaggcggtc gcggcatcgt
    38761 ggaccatttt cacctgcacg gccgcgtgga cgtggaagtt ggcactttct ccaaggcgtt
    38821 tggcgtggtt ggcggcgtgg tggccggcag cgcgcgcatt attgagtggc tgcgccagcg
    38881 cgggcgcccc ttcctgttct cctccgccgt gaccgtaccc gacaccgccg cctgtctggc
    38941 tgcggttgac ctgctggaag aatctaccga gctggtggac cggctgtggg cgaacgcgcg
    39001 ttacttccag gcggagatgc gcaggcttgg cttcgatacc ggcgcgaccc aaaccccgat
    39061 tacgcccgtc atgctcgggg aagtgcagct ggcgcgcgaa ttcagccggc gcctcttcga
    39121 cgaaggcttg ttcgccatgg cgttgggctt ccccactgtg ccgcagggca tggcgcgcat
    39181 ccgcgtgatg ctctccgccg cgcacagcca aagcgacctg gaccaggcgc tcgaaatttt
    39241 ccgcaaggtc ggcaaagagc tgggcgtgct gcgcggataa gaccatctct cagaataaaa
    39301 caaaacgctc catccaaaag atggagcgtt ttcattcacg catgccgaag gtgggaatcg
    39361 aacccacata cccgagggta cacgattttg agtcgtgcgc gtctgcctat tccgccactc
    39421 cggctggcga gaaaaagtat acccaatcgc ccgccgcggg tcaagctttg cgcattaacg
    39481 aggggtgtag gcgataaagt tatcgaagcg cacggtcaca ggcggtacat cgtaggtgcc
    39541 cacaaccagg ccgacgtctc cgctctccag gctgctgtct tccacgctgg cgataaaatc
    39601 gccgtttacg tacaaagaca gcgtctcacc gatgcagctg gcggcgatgt gattcaggtt
    39661 ttcatcaaaa ccggctaact gcatctcgcc gtacagcagc tcagcctctc cgccgacata
    39721 cttcagaatc tcaacatagc cgtcgttggc aatggtaagc gagtagtagt tatcctcatc
    39781 aacatagcgg cacatcacgc cgtagttgtt ggtcaggtcg ccgtcccaca gcagggcatc
    39841 cacctctata accacatcag gggtgtccag cagcgtggat tcagtccaga ttaaccagct
    39901 ttctttcacg acagtaaatt ccatgattcc acccaggttt ttgatggagt tgccgtcttc
    39961 ttcgccggtg taccaggctt tgtcggtggt cgaaaagtcg tcttccaaca gaatctctcc
    40021 ttgctctggg agcccgacct gagttgaaag cggaacggct gatttcgcgg tgggggaagg
    40081 aatcagaatg ggctctgtgg gcgccagcgc ggcgctggtt tccgtcggca cagggctggt
    40141 ggtgggaact tcggtaggca ccagaccaag ggtgggcgcg gcggctatgg ttgcggtcgg
    40201 ggctgggttc ctgcccatgg agcaggcaag ccccacactt aagagtaatg ctgcaatcaa
    40261 tgagattttt ttcatcatag gctccgtcga gagtaagaaa agcgggaaac tagggaaagt
    40321 ttatcatgaa agaggggtat taagaaaaaa agtatgcata ccctcgcgtc cctcagcaga
    40381 tttttacccg gacagcgggg ggagtgtata atccaattga acccggcgcc ttagtttccc
    40441 ggtcagccgc ccgacattca actctcagga gcttaccatg cagttaggca tcatcggctt
    40501 gccgcagtca ggcaaaacaa ccttatataa agcgcttacc cgcgcggata tccccaccga
    40561 tatttcctcc ggtcagatgg aagtccatac cgcggtggtg gacgtgcccg acccgcgcgt
    40621 ggaccagctt tgcgaaattt accaccccaa aaagattgtg cgcgccaagg ttacctatgc
    40681 cgatattgcc ggcttggacg gcagcgccgg gaaaaacggc ctttccggcg cgctttccaa
    40741 catgctctcc cagatggacg gttttctgca cgtcgtccgg cagttcgaaa accccagcgt
    40801 gccgcatcag gccggcagcg tggaccccat ccgcgatatc cagaccatgg ataccgaatt
    40861 tgtgctgaat gacctggtca tggtggaacg ccggctggaa cgcctgcgcg aggaacaccg
    40921 ccgcggggtc acccgcgacc gggccttgat ccaggaggat atcgacctgt tcgagcgcct
    40981 gcaggccgtg ctggaaaaag agctgccctt gcgccactac gccttcacgc ccgaagagga
    41041 caagctgctc tccagttttc agttcctcag ccgcaagccg caaattgtag tcttcaacca
    41101 atccgaggac caaaccccgc tggaatacca cagcccgcac ccgcatacca gctccgtgag
    41161 cttgcctgcc aagctggaaa tggatatcgc cgcgctggac ccggaggacg cagctgtttt
    41221 catggcggaa tacggcatca ccgagcccag cctctcgcgc ctgattcgcc tttcctacga
    41281 cttgcttggg gtgcattcct tcttcaccgc cggcgataaa gaagtgcacg cctggaccct
    41341 gcgcaaaggc tccaacgcgc gtgaagctgc cggagtgatt cactctgata tggaaaaagg
    41401 cttcatccgc gccgaaatca tctcgtttga ggatatgatc accttcggcg gcaccgcaga
    41461 ggcgcgcgcc aagggacgcc tgagggtgga ggggaaaacc tatattatcc aggacgggga
    41521 tattattgaa atcaggttca atatttaagg aaaagcagcg ccgctaagtc cgcccgcaat
    41581 ccaatagaaa aaaacagcgc ggtattctta gggtaccgcg ctgtcgcgtt ttatcgggcg
    41641 aattccgggg gattattttg gcgcagtaag ctggcgaaat ccgacaggtt cacccgcgcg
    41701 gtcaaatcaa aggaaagcgg ggtaacgctc accttgcggt caatcttcac gacgtgcagg
    41761 tcgctgtccg cttcgggctc gttctggtct aacctcacct catacgttaa tttgcgtgag
    41821 ccctgcgcct tgtccgggtc tgccggcacc gcgtaaaagt agcgttcgcg cgattggcgc
    41881 gtgaccaccc agggtgtctc gggcgtggca tcgtttggca cgtccacctt gagcacgcca
    41941 acatcgaaag gcatcgcggt ccgcagcagc ttttcggcga agtagcgcgt gaaatatccc
    42001 gcgaccgaaa aatccacatc cggcgaaagg ttgaaatgct ggctgaccgg cgtttccagc
    42061 gacacagcca gcgcggagat gcccagggta gcggcttcca gcgctgcgcc gaccgtcccg
    42121 ctgatggtga tgccggaggc gacgttttcg ccgtaattaa tcccggaaac gaccaggtcc
    42181 ggcttgaagc cgagcacctc ataatagccg tgcaaaaaag cctgcgctgg tgaaccgccg
    42241 acagcgtata cctgccattc tttgccgtta acagtcatcg tgcgcgcttc gatgatgccg
    42301 tcggattcgc gcgggagggc gcgccccatg gaggtctgct ggacgcgcgg tgccacgaca
    42361 tgcacaaaac ccagaggcgc gagcgcttcg gcggctgccc ataagcccgg ggagttgatg
    42421 ctgtcatcgt ttgtcagtaa gatttgtggt ttttcgtttg tcttcatccg cgctattcta
    42481 atcccgaaaa gcgcctgcgg caacaagact gcccccgcgc ggtttttcag cgcctggagg
    42541 actgcccgcc ggcagctttc gcgccggaat ttacatcttt tcgcggtagt tcttcaggat
    42601 ttcctgccct ggcagacgaa tcgccttggg ttcgcacacg ctcttgcaga aacagcagcc
    42661 caccaggcac aggaaaggct ccgccacaca aatatgtccg ttttcgtcgc gctcaaagac
    42721 ttcgcgggcg caggtttcca ggcaggcgcc gcaatcgttg cacagttccg gcgcgatagc
    42781 ggggaaccag gggatttgtt cgcggggata gccgcgccag ctgccgtaat cgcgcgccac
    42841 cgcggctttt ccgctggcct tcaggcggaa aaagcggctg tattccatgc acagcgcgcc
    42901 ggcgtactcg ggtacggcgg gggtcggaat aaagttctgt ttgcgcaccc cggcaatcag
    42961 ggcgtctttc agcttttcat ccgtgggcag ccagccctca gcgtagagct ccgcgaagag
    43021 ttcttccaag cccagcaagc cggtgggcaa tccgcctata ttcagcatgc ggtaacttga
    43081 gacaggcatt attctccccg ctgcgcggcg cagtccccag cggcggtttt caaaaagctg
    43141 accattgcgg tcagaccgtt gctgcgctgg gaggacagat gccgccagaa acccagcgtc
    43201 tcgaacaggc tcaggtccac gttttggtat gcttccgccg gctgaccgtt caggagttcc
    43261 tgcagcaagg ccgccagacc cttcacgatg agcgagtcgc tgtccgcctg gaaatacaga
    43321 ctgccctcgc ggcactcggt gacgaaccag acgctggatt ggcagccttt gaccaggtgt
    43381 tcatcgtcct tcaggtcttt cggcatgtcg ggcagggact gacctttttc aatcaggtaa
    43441 tcgtattttt cttcccagtt ttccagcaag ctgaattcag cgctgatttg ggcggcaatt
    43501 tccgcggtag tctgcattgc tttacctttt taacatcttt atcacgcgcc ggataccgcg
    43561 cgtgagcgcg tcgatatcat cgcggttgtt gtagagcgcc agagaggcgc gcaccgtgcc
    43621 cgagatggac agggcgtcca tgattggctg ggtgcagtgc tgcccggtgc gcaccgcgac
    43681 ccccgatgta tccagcagga tgcccatatc gtaatgctgg ataccttcca cgttgaaggg
    43741 caagatgctg gagcggtgct ttgggttccc atacagcgtc aggcccggga tggcgcgcag
    43801 ctgtgagatg ccgtacgcca gcaggtcttc ttcgtaggtc tgcagttcct gcgggtcgaa
    43861 ttggttcagg tagtccaccg ccgcgccgag cccgatggcg ccttccacgt ttggggtgcc
    43921 ggcttcgaac ttgtgcggca gctgcgcgaa agtcacgcgc gtgctgctga cctggtcaat
    43981 catttcgccg ccgtacagga agggcggcag cttctccagc cagctttctt tggcgtacaa
    44041 aacgccgatg cccattgggg cgtacatctt atgcccggag aaggcataaa aatctgcgtc
    44101 cagggtttgc acgtcgcgcg cggccaggtg ctgcacggac tgggcggcgt ccacaagcac
    44161 gggaacgccg gcggcatgcg ccagaggaat cagggtttcc agagggttga cggtgccgag
    44221 cgagttggaa acctgattga gcgccagcag gcgggttttt tcggtgaaca tgccctgcag
    44281 aacctcaggg tccagctcgc ccgtcggggt gaagggcagc acgcggaagc gcgcgccttt
    44341 ccgttggcaa agctcgaacc agggcacata attgctgtgg tgttccagct ggctgacgat
    44401 aatctcatcg ccgggatgca caaaggcttc gccgaaagaa ctggctgcca gattgatcga
    44461 ttccgtggtc ccgcgtgtaa agatgatttc gtgggcgtgc gcggcattga taaagcgcgc
    44521 caccttctcg cgcgcggctt cgtacatgtc cgtggcgcgt actgccagcg tatgcccgcc
    44581 gcggtgcacg ttgctgttta cgaagctgta gtaatcgcgc agggaatcaa tcacaacctg
    44641 gggtttttgg gtggtcgccg cgttgtccag gtagaccagc ggatggtcgt tgatccaggt
    44701 agaaaggatt gggaaatctg cgcgcgctgc ggaaataggt gtggacatgc ctgcctctga
    44761 taatttcaga cccattgtat catcacaggg gctggtcttt gcctgcctcc aggtgtacaa
    44821 gcggcgcctg cgcagcgaaa aggggatgcg atacaattgt taacaaacgg gacctgctgc
    44881 ggatgacgct cacctttgcc gagaccgcgc gcccgtacag gagtaggcat gcccgaaaaa
    44941 atcttggtcg ttgatgatga aatttccctg caggaaaccc tggcgtataa ccttaccaga
    45001 gaaggctacc aggtggaggt ggcgggggat ggaaatcaag cccttcattt agcgcgtaca
    45061 tccaaaccag acctggtgat tctggatgtg atgctgcccg gcctggacgg gtttgaggtt
    45121 tgccggattc tgcgccagga agccaacctg cctgttctga tgctgaccgc gcgcgatgac
    45181 gaaattgacc gcgtagtggg tttggaggtc ggtgcggacg attacctggc aaaacccttc
    45241 agcatgcgcg agctgatcgc aagggtaaaa gccctgctgc ggcgcgtgcg cctgacccga
    45301 gaggacatgg tcaccgaaaa gcaggaggcc gaacagccta agctgattac gtttgataac
    45361 ctggtcatcg accttacgcg ccgggaagtg aagctggacg ataaagtgct gccgctgaaa
    45421 cccaaggaat atgagctgct gctctttatg gggcagcaaa aagggcgcct gctgacgcgt
    45481 gagttgattc tggagcgggt ctggggttgg gggtttgtgg gggattcccg cacggtggat
    45541 gtgcacattc gctggctgcg cgagaaaatc gaaccgaacc ccgaaacccc gcagcgcatc
    45601 gtcactgtcc gcggggcagg ttaccgcttc gaaggctgat gatgggtttg ttgagcaaac
    45661 ggaggtttat ccttccgtac gcgtttatct taatcggcag catattgctc tcggcggtgc
    45721 ttgttccgcc ggcagtccgg tctgcctacg cgcgcagcgc tgaaagcggc ctgccgcctg
    45781 atttcaccct gatgtacctc tccatcggag ctgtggggct gcttgcgctg gccgccgcga
    45841 tgatagccaa cagaatctac gcgaaccgca acctcaacat aatccgccag cttaccgtca
    45901 cggcgagcca actcggggag ggcaaatacc gcagcattgc cattccccac caggtggaat
    45961 ccgtgccgga gatgcatgcc ctgagccagg cgctggaaga gaccgccgcg aagattgagg
    46021 cgcaaatttc cgcgctttcc tcggaacaag tgatgctctc gacagtgctc gggcaaatga
    46081 ccgacggcgt gctgattgcc aatcatgatg accgcgtgca gctgcttaat cccgccgcgg
    46141 aaaggctttt tggctttaaa aatgccggcg ctttggggcg ctctgtcata gaggtcattc
    46201 gccaccacgc gctggtggac ttatggaact ccaccaagga aggcgaatca aaaacaatta
    46261 cgctggaaat gggcgccgac cataaattcc tgcaggtcat cggtatcccg ctgggagagc
    46321 agctgcccga acgttccatg ctcctgtttc aagacctgac gcagctccac cgtctggaaa
    46381 ttgtgcgcaa agacttcatt tccaatattt cccatgagct gcgcaccccg atggcgagcc
    46441 tgaaagccct gagtgagacg ctcctggatg gcgcattgga cgacccgtct gtcgcaaaac
    46501 gcttcatcgt gcgcatggat accgaagtgg acaacctgac ttatctggtg aacgaactgc
    46561 tggagctttc ccgcattgaa gcaggcagaa ccaacctgga gttccaacgc atccaccctt
    46621 gcgacctgat gagaaggccc tatgaacgca tgctgctgca agctgagcgc gccggcttga
    46681 gcctgacgat gaactgcaac ccggacttgc cggcggtgct tgccgacccg gaccgcattt
    46741 cccaggtatt tatcaacctg ctgcataacg ccatcaaatt tacgccgaat ggcgggcgca
    46801 tccagatgga tgcctgggtc tttgaagata aggtagtctt tatggtgcgc gataacggcg
    46861 tgggaatgcc tgaaaaagat ctgaaacgca tctttgagcg cttttacaaa gttgaccgcg
    46921 cgcgttccgg gggcggtacc ggtttggggc tgtccatctg caaacacatg gtggaagctc
    46981 acggcggtta tatctgggcg gagagcgaag aagggaaagg cagcagtctg ttcttcgcga
    47041 ttccaattgc gttcgggtag gtgaagggcg tccgcgtgaa cccgacacaa aacattgaca
    47101 ggctttttac cgcgcattaa cccattccta atcatgactg gtgcataata aaaacgggag
    47161 agcatgcctg ttgttacgcg tgtaaaaaca cagacaatag cgtacaatag gtttacacag
    47221 aaaacaccgg acacagagga attcagagat gccccgcctc acattggatc gagaaattcg
    47281 ccttattcag gatgaaacgc tgctccttgg cagcctggtg gaacaggcgg tgctgaacgc
    47341 tgtcgacgcc ttgaaatcgc gcgacgtctc ggcggcgaga gccgtcatcc gcagggatgt
    47401 cctcatcaac gacaaacgct tcgcgattga aaaccgcatc ctgattctgt tcgccaccca
    47461 atccccgatg gcgcgcgacc tgcgcttgtt ggcagccgag ctggaactca tcaccgagct
    47521 tgagcgcatc ggcgattacg ccaaaggcat cgccaaggtg gcggaagaac tggctgatga
    47581 tgaaacgccc gttcctatcc gcgagatttg cacgatggcg gacctggcgg tcagtatgct
    47641 gcaccgcgcg ctgagcgcgt tcatcgctga agacctggat accgccagag ccctgcccgc
    47701 cgaggacgac caggtggacg aactctacaa ggtcatctac cgcaaaattg tccagggcat
    47761 gattgccaac ccgagctcga ttgaccattc caaccaaatt ctatgggtca tccataacat
    47821 cgagcgcacc gccgaccggg tcaccaacat ctgcgaacgg attgtgttca tcgctaccgg
    47881 cgaactgctt gagatggaca gttccgacga cgagcttgag accccggaag cctgaagacc
    47941 cgcgccgcat tatcccggcg tttatcttat aatgaatatt atttccggta tccgcgccgg
    48001 gcgggagcgc gcagttcact tatgctcttc gttttcccgg gcgcttatcg cgatgaatca
    48061 taaggagagc attccgtgtt aaaacccaat accacgaaag gaaagttaat cgtcgttgaa
    48121 ggcatcgacg gttccggtaa atcaacccag attgatttgc tgttcaagtg gctgcaatcc
    48181 cagggttatt ccgtgttttt cagtgaatgg aacagttccc ccctggtgaa aagcaccacc
    48241 aaagctgcca agaaagaaaa gtccttcacc cccaccacct tcagcatcct gcattgtacc
    48301 gactttgccg accgttggga aaacagcatc tatccgctgc tgaaagccgg cgcgattgtt
    48361 ttggcagacc gctatgtttt caccgcgttt gcccgggacg tcgcccgggg ggtggacccg
    48421 gagtgggtgc gcaatatgta ttcctttgcc atcaaaccgg acgccgtttt atacttccgc
    48481 gtcccgctgg aagtggcggt ggagcgcatc accgctgcgc gcgccagcct gaagtattac
    48541 gaagccggga tggacttgaa cctttcagag aatatcaatg aaagcttcaa gcttttccag
    48601 ggcaaaatac tggagcaata cgaccagatg gtggatgaat ttggattgac cctgattgac
    48661 ggcacgcagc cggtcaaaga ccagcaaaaa aaagtgcgcg cgctgatcaa gaagatactg
    48721 cgcggctggg aaggcctgcc caatcccaat aagccggcag gatccgctcg gcccgctgcg
    48781 gaaacaggag gtaaatcatg aaaggcgcga acttttatgg tgcaggcttt ccctaccggg
    48841 atattcagca gctgcccggc aagctgattg tgctggaagg gaccgacgga gtcggccgct
    48901 ccacgcagat taaactgctg cggcgctggc tggaggatga aggctatgcc gtctccgata
    48961 ccgggctcac ccgttccgcg ctgaccgctt ccggactgga agccgcgaaa agcggccata
    49021 ctctcggtcc gctgacctta agcctgtttt acctggcgga ttttgccgac cgcctggaga
    49081 atttgataat cccggcgctg caggccggct ttgtggtgct ctccgaccgg tatttctact
    49141 ctgtcatggc gcgcgatgcc atccgcgggg tagataaatc ctggtcgcgc cagctgtacg
    49201 gcatcgcgct caggcctgac ctgattcttt acatgaaagc ggatgtgccc gtgctggcgc
    49261 agcgcctcat ccacggccgc gggctcaact actgggaagc gggtatggac ctgcacctgg
    49321 ccgataacat gtatgacagc tttttggctt accagaattt gctcaccaaa gaatttgacg
    49381 agttggcgct gacttacaac tttgtgaccg ttgatgccaa ccagaaaccg gacactattt
    49441 ttgaagacct gaaagtgcac attctggaac tgctgcacaa ccggctgacc aagacaaaaa
    49501 agcccagata aacagccggt cttgaaaact taacagcgct ggcgcccgtc cggcggaagg
    49561 agcggaaaca atgaacgcta atgccgacgc gattactgtc tacggtgcaa aaatcatcag
    49621 ccggcatgcc gccgcgatgg cagcggaaat ggagggggtg cgcaaagcgg aggatattga
    49681 gtacatccac cgaatgcgcg tagcctcgcg gcgcatgcgc agcgccctca cgattttcaa
    49741 aggctgcttc gacaaaaaaa agtttaatga aatcgatcgc gacgtacgca aggtggtccg
    49801 cgccctgggc gaagcacgcg acctggacgt gcagctggag gtggtgcgcg aggcggaagc
    49861 acagttcaac acccccaggc ttctgccggg gttgaagcgc ttgcagctgc gcctgaccca
    49921 aaagcgcgcc caggcgcagc agcacgtcgt cagcgcgatg gaccgcctgg cgcaggacca
    49981 gctgctggag cgcctggaag cctggtacgc gcctctgcag gcggcggccg agggcgtgta
    50041 cctttattcc cccgctttgt accagctggc ttttgaaagc gtccaggccc gcttgaaaga
    50101 actcctggga catgtgccct acatcaacga tccggcgaac gtggcggagc tgcacgctat
    50161 gcgcatcagc gccaagcggc tgcgctacac cctggaagcc ttcgatgacc tgtatggtgg
    50221 gcgtctgaag ccgtttatat ccacaggccg caaattgcag gaccagttgg gcttgattca
    50281 tgacctggac gtatggatat ttttcgtgcc ggccttcatt gaggaggagc gtgcgcgtat
    50341 cgttggctac tttggcagcg acggaccgct caagcgcttg ctgcccggct tgctgacctt
    50401 ccagaaatcc cgccgcgaaa tgcgcgatgc cgcctatgcg gaatttctgg tttcctgggc
    50461 ggaaattgag gcggcaaaga cctgggaaga gatgatgcgc ctgctcaaca ccccgatgga
    50521 cctggattcg gcgctgcgcc tgctggaggc cgcccgggcg ggccagaacc cggaggcgca
    50581 gccgggcagc acggctgcgg atgactgaag tttcgcgtca ggtctcgatt gcgtgcagta
    50641 aggcaggagc gaaacccgat tgtagaaccc ttcgaacctc gccaagttcc acgcacccaa
    50701 gaagcggcct ctacccggcc tgtcattctg agtcagcctg caaaccagct tgtcatttgg
    50761 attgctatga ctatgtcagg agcgcaggat ggattgaatg aacatcgggg atgtatggtt
    50821 tgccggatta atccaacgaa agcaggacgc taaccaggtt gcaggtcggg tagagcggca
    50881 gcgcaacccg acagaacgta gccgtaggtc gggtggagcg gcagcccaac aaaacgtggt
    50941 cg
//
LOCUS       DAOQAD010000002        50837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_529_length_50837_cov_5.501851, whole genome shotgun sequence.
ACCESSION   DAOQAD010000002 DAOQAD010000000
VERSION     DAOQAD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 50837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50837
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_529_length_50837_cov_5.501851"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKL60_00240"
     CDS             complement(<1..361)
                     /locus_tag="PKL60_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94593.1"
                     /translation="MPIAITSVRYPWLQPAKSSGVIYALRIGRKNATSSGVITMPGTP
                     GKQRDDSFTGIAFDCRLKERVNQKKTASDHLSEERLESEQSAERRVGETGIKSFLRIN
                     RKHGEAGICLYPSGQTRN"
     gene            1275..1487
                     /locus_tag="PKL60_00245"
     CDS             1275..1487
                     /locus_tag="PKL60_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNW94594.1"
                     /translation="MSEREVGTVKWFNSEKGFGFIARNSGGKDVFVHYSAIISDGFKT
                     LQENQSVEFTVAQGAKGPQAQDVKVL"
     gene            2561..3397
                     /locus_tag="PKL60_00250"
     CDS             2561..3397
                     /locus_tag="PKL60_00250"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNW94595.1"
                     /translation="MSDTITLQEIDSAAACVRGRISIQPQIALILGSGLGPLADEVES
                     PAIIPTNEIPNWPVSTVQGHKGRLVIGRLMGKPILVLQGRAHYYEGYSMPRIGLPVRV
                     MQRLGVKTVVLTNAAGGINPDFYAGDLMLITDHLSLLPMTGPNPLRGPNLDEFGPRFP
                     DMSQVYDKELLRLAQEVSDAQQINVRRGVYAGLSGPSFETPADCRFLRLAGADAVGMS
                     TVPEAIVARHGNLRVLGISGISNKIDINGNNVASHEEVLEAGKVIVPKLSALIKGVLA
                     RL"
     gene            3450..4148
                     /locus_tag="PKL60_00255"
     CDS             3450..4148
                     /locus_tag="PKL60_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94596.1"
                     /translation="MDAIVKFLADLEVPIYLILGVVAVIYLRRLALALDERKSSIFGL
                     EREAAQTKVVSAATVMILVALLAVGEFIVATFLAGELAQQPLYATPTIAVLTTPTTTL
                     LPAAVPTDATPTPTPYPQAQIEGIESNCQAEVLEISEPKHGDEVSGVVELIGSVNTPN
                     FGSYEYEYSVMGQPNWQTIAAGSGTRVQSSLGFWYTTDLEPGDYLLRLVALDNQGERT
                     AACVIVVKVSAPQE"
     gene            4163..4702
                     /locus_tag="PKL60_00260"
     CDS             4163..4702
                     /locus_tag="PKL60_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW94597.1"
                     /translation="MAELEIKAARQADLDRMLGLNHHSSSEYAYKLELTNEKGIFTHS
                     FQRVFLPRAVNIKYPKDETALINSWGEASAIFVGLVLGELIAYVALETGRIKDTARVT
                     DLVVAPVMRRKGVGSGILLACEGWAHDQKARRILMEIQMRNDPAIQMVQKLGYQMCGY
                     LDQYFPNHDTALFFDKPIS"
     gene            4732..6681
                     /locus_tag="PKL60_00265"
     CDS             4732..6681
                     /locus_tag="PKL60_00265"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNW94598.1"
                     /translation="MLTLTGILKIIAQILNAGVAITAIALAMYALSFNFRDNTARTFM
                     ILLALVAVVFSSDTIANISNSPAIIQAWLQLRWAAMAFLPAAYFYFSDALLTLTGRPS
                     RGRRTWLVRIFMLISLAWAVLIPLRITVGPLADQANPVAYLQTGPVSVAFGVYYLAVL
                     ALSFYNLARAVLRSNTTTTRRRLFYLFIGASAPAVTCVLYLFHGSALFAYNPVFFWGL
                     TIIGAILTGISLVVIAYVVSFFGLNWTDRAIKSRLFRWLMRGPFVAMVTLGATTVVRR
                     YGLTLGDPYIPYVPVVMVGTILLLEYFITLLAPGLEKPLFFGADREDLSLIRTLEERM
                     LTEKDLDQFLEIIAASICDQLQVRGAFIAVVENGTVDYIIHAGDRHILDKLPETDTLL
                     RAVQAERKDPREMLTFESFRLIPLAYQQDIEQTRLFGVCGFPAEDTDTFFPEQLSAVR
                     LLADRATLALKDRALQLQVLNSLSVLQPEVDYIQELIASSSYNQKGIYRNGSTQVPGE
                     LTDWVKDALTHYWGGPKLTNNPLLNLRLVENASENYDGSKANALRAVLKQAIEHIKPE
                     GERKFTSDWILYNILDLKFIQGEKVREVARKLAMSEADLYRKQRVALESVARIIMRQE
                     AETAAGEKQPETDSEAPASASGDAL"
     gene            6742..9021
                     /locus_tag="PKL60_00270"
     CDS             6742..9021
                     /locus_tag="PKL60_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNW94599.1"
                     /translation="MSPTKAKASETKNGGSPAREHSPSAAQSPAPKPGNKGWFDPSSI
                     GWDLVGVLLLALGFVLLLAVLGFTRGTILDLILGAARRWFGLGRYLLPLAFLAGGWLL
                     LLWRKNEHSSVSAGRVVLVELGLLFLLGALSAFTRETVLEIEAGTSLGGSTGWGLAHP
                     LAGLVGSFGAGILLGLLSLLFLLYGLNFFRRIEAWARARVGAAPSASETPAPVLETSG
                     RTRTAELPRQTPAKEVKTQQTLPLTYQKVTKETIPEKPPEAPLSRPDVLPPLSLLETE
                     KPAVVNEAAIQMNAAILERTLGEFGIPAKVVGHRVGPAITQFALEPGYIEKNGSPEER
                     QKVRVSKISNLSRDLALALKAERLRIEAPVPGKSYVGIEVPNAVHTIVKLRPILESEQ
                     FQRVNAPLAFPLGKDVSGAPIVADLAVMPHLLIAGTTNSGKSICIAALTVCLVMNNKP
                     ENLRLVMIDPKRVELTRFNGLPHLLGPVETDLERIQSLLRWAVGEMEARYKILEEAHS
                     RNLDSYNARMTHSGKPTLPRIVILIDELADLMLAAPEETDRALVRLAQKARAVGIHLV
                     VATQRPSVDVITGSIKANFPARIGFTVASLMDSRVILDSPGAETLLGKGDMLFLNPLT
                     GQLERSQGVYVSDGEIRRVIAWWQQQTGGAEKKTAPAKESPAVNEGPASPWEQQVSED
                     SEDSDETLVKQAVEVLRRTKRASASFLQRQLHLSYPRAAWLVDELENRGIIGPAQSGG
                     KNRQILLDENADETDEAVE"
     gene            9040..9660
                     /locus_tag="PKL60_00275"
     CDS             9040..9660
                     /locus_tag="PKL60_00275"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNW94600.1"
                     /translation="MNEQKTNQKSFEFLLRKTFSGILDGIAGFLLGLGLKPNHITLAG
                     LCGNVVAALLIGSGYLVWGGVTAALMGPLDAVDGAMARKLGTPTRFGGFLDSVIDRYD
                     ELLLLGGVLVFFTREANTAGIYLTYAAAAGSVLVSYMRARAEALGFDGKVGLLTRVER
                     YIILVIGLLLNLTIYSVGIIAVLGNATAIQRLFYVKKQTGENTRRE"
     gene            9678..10715
                     /gene="lgt"
                     /locus_tag="PKL60_00280"
     CDS             9678..10715
                     /gene="lgt"
                     /locus_tag="PKL60_00280"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNW94601.1"
                     /translation="MPQGFQIGPLTIRYYALIILAGALLGAWLASVEARRRDKDGNLV
                     WDILPWLLVGGIIGARLWHVFTPSASNIAMGLTTKYYLTHPLQILMIWNGGLGIPGGI
                     IGGGLALLIYCKVTKQSFLEWADIISPGLLVGQAVGRWGNFVNQELYGSPSNLPWAIT
                     IDPLYRLDGFKEVSKYHPLFLYESVLNLLAAGILLFVGRKYKNKLYRGDIFLLYLVLY
                     PVIRFGLEFLRLDPSPVAGFNINQTVMGIVALLSVALLVLRHTIRRPKAVQELETEGI
                     DISDMQTVAIEEAGLESDTAALGDDGLEPVQGDAGLEGPFEPLTDMTEEAPQIEPEGS
                     NAEDETHFPED"
     gene            10870..12084
                     /gene="tyrS"
                     /locus_tag="PKL60_00285"
     CDS             10870..12084
                     /gene="tyrS"
                     /locus_tag="PKL60_00285"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNW94602.1"
                     /translation="MNIDEQVEYLMQGADYGDTAVEQAMAVELRERLIEAGREGRPLQ
                     VYCGYDPTSTDLHLGHTVTMRKLRQFQELGHEVTFLIGNFTSLIGDPSDKDKLRPQMS
                     TEKVMHNAETYAEQAFRVLDPERTKVRYNSEWLSRLTMEDLFRLASNFTVQQFLTRDN
                     FRKRWDNAEPIYLHEFFYSLMQGYDAHHLNADVQVGGSDQLFNIMTAARKLMTFFGQK
                     PNIGIILGILPGTDGEVRMSKSLGNHIPLNTSAEDMYGKVMSIPDKVMPVYARLATDW
                     PPETLNHFLTALESNSMHPRDAKMKLARGITAVFYGEEAAESAQNQFIAMFQKGDAPD
                     EMPEYRLAGAENLLEVMVKNKLAQSRNQARRLVEQQGVKIDGITVGDVTLIIDQPCIL
                     QVGKRHYLRILR"
     gene            complement(12089..14695)
                     /locus_tag="PKL60_00290"
     CDS             complement(12089..14695)
                     /locus_tag="PKL60_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNW94603.1"
                     /translation="MSETAAPKKTATLPAPLWLVGLFVSFYAFALALAPAVRTHASDF
                     QPELRYLLPLLGWAAGIAALQFAADKLLPRHDPTLIPLVGALTGWGLLSVWRLSPALG
                     GKQLLWFLVGVLVMIGGMTIRDLVALLKQYKYVWLALGLLLVALTLFVGVNPSGSGPR
                     LWLNLFGVYLQPSEPLKLLLLVYLAAFFADQIRPNVSIAASILPTLLITALVGVLLVI
                     QRDLGTATLFAVLFLFMLALTTHRRRLLWLIPLLIITAGTAGYFLFDIVRTRVDIWLN
                     PWLQPAGASYQLVQAQIAIASGGLIGTGPGLGSPQFIPVAVSDFIFSAIAEETGLLGT
                     SALLLLILLLVMRGVAISRRTKNTFGRYLAFGISAFFALQSFFIIGGNLGLMPLTGIT
                     LPFISYGGSNLLTCMACVLLLLRLSAEPGKRMSAEGSHSTYQFLSAVLILLFVLLQGK
                     NAMISVWNRDTLVSRPENLRWAVYDRYSPRGNIITTAGDPLAVTTGEPGAYSRELLYP
                     PLSSVVGYTNGLYGQSGLERGVYSFLRGYGKPFSEVWKNQLLYNQPPAGSDVKVNLNL
                     SLQQAADRLLEGKKGALVLLNAQTGEIYALASHPYFNANTLEDDFNDLMSREDAPMLN
                     RTTQAAYPVGTLLNLPALAAAWTDPQIRYQIPELSPRLDLPCYQAMKAASSRVNVLQY
                     GCESASADLLRGTTPEELADIMRAFGFFREPAVAVESAQAAPDPALGDDLAVWLQTAA
                     ETNVTPLQMALMAAALTNDGLQPTPKLVNSYQSENGAWVAFGGNTSALEVFNSDLALQ
                     LRNQFLAESQSIWYLNGHAALESGETLTWYIGGSTPQWVGVPLAIAVAIEGSEPALSQ
                     QIGSQLLTLMTP"
     gene            complement(14698..15579)
                     /gene="mtnP"
                     /locus_tag="PKL60_00295"
     CDS             complement(14698..15579)
                     /gene="mtnP"
                     /locus_tag="PKL60_00295"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNW94604.1"
                     /translation="MEAGKIAFGIIGGSGLYAFEGLENKKTVIIDTPFGLPSSPIVLG
                     EVHGKPLAFLARHGIGHTISPSEVNYRANIYALKQLGVTKIISVSACGSLQEKFAPGD
                     VVIPDQVFDFTHKRERSFFGEGLVVHVGTAEPFCPNLSAALAKALKAEGGTVHEGGAY
                     ITIEGPRFSTKAESNTFRAWGMSLVGMTASPEVFLAREAEICYATMAHVTDYDVWHTS
                     EDPVSVDMVVRTLARNTDLAQRALIRLVDILEQSETDAPACACQDALKDALITSPGKI
                     RPETREKLQLLISKYVK"
     gene            complement(15598..16029)
                     /locus_tag="PKL60_00300"
     CDS             complement(15598..16029)
                     /locus_tag="PKL60_00300"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNW94605.1"
                     /translation="MNAGILLVLRILFLIALYGFLGLSLWVIWKSTVTKAAQSVQELV
                     PPLSLSSTVMESTNAQVFITPKVTVGRAPECDFILPHEMVSSLHGSFSYHHQQWWYED
                     LKSTNGSYLEDLRVEEPVVVKDGDVLFCGEVDINISIKPLS"
     gene            complement(16026..16787)
                     /locus_tag="PKL60_00305"
     CDS             complement(16026..16787)
                     /locus_tag="PKL60_00305"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 domain-containing protein"
                     /protein_id="HNW94606.1"
                     /translation="MLLETIENKLRALFEGTLDRILYPGLASSLSSQLVALISEKIAA
                     QPPNTHRAPDLIDILISPDKWDAWQSARPALDDAARELEAYWAEQGYTFLHSPRIKVI
                     ADPELSAEAIAIHVDYAHSDRQQAKTSLQTVPRPLAEEPIPTHAYLIINGKEQAALEK
                     AVIKIGRRSTSDIVLNDPMISRDHVQLRAEQGKYLLFDLSSTGGTFINNRQVHTAVLK
                     PGDVIRLGKTLLVYNQELPGTDETKISLIESQPKP"
     gene            complement(16920..17984)
                     /locus_tag="PKL60_00310"
     CDS             complement(16920..17984)
                     /locus_tag="PKL60_00310"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNW94607.1"
                     /translation="MEEIIVALHMHTVYSDGNGKHADLAAAALKAGLDAILVSDHNVW
                     VQGMEGYLQKGKKRLLTLVGEEVHDQTLPKGKNHLLIFGSTRELARFAPDPQQLIHQA
                     GASGGLTFIAHPIEDALPAFGEAAFNWEDWQVSGFTGIELWNQMSEFKTRATSLPKTV
                     LHAFFPQLMTFRPLESTLKLWDELILKHNKPIVAVGGADAHEMQIRKGPLRVVLYPYE
                     QQFRSITNHLLLPKPLTGNLTQDKTLIYDALRAGHSFIAYDLPRSARGFRFTVNSNEG
                     TFWMGDQVSAEDGLTFQVRLPARTHVRLLRNGTVVKEHTDREVLTYLTKEPGSYRAEV
                     YLDYLGMHRAWIFSNPIYAL"
     gene            18596..18820
                     /gene="secG"
                     /locus_tag="PKL60_00315"
     CDS             18596..18820
                     /gene="secG"
                     /locus_tag="PKL60_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNW94608.1"
                     /translation="MNSFLNIAIMITSVALIASILLQSKGIGLGSLAGGDTGGVYTKR
                     RGVERVLFFVTIGLAVLFVVLALVSVLISG"
     gene            18912..20582
                     /locus_tag="PKL60_00320"
     CDS             18912..20582
                     /locus_tag="PKL60_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNW94609.1"
                     /translation="MRKFRWQLLIILLTGLVVGLLLVFQQGTNLPEAQNIPSPVTGGI
                     YTEALVGNFLRLNPILDMVNQADKDVDRLLFNGLVRFDSSGMPQPDLAETWSYSSDGT
                     RFTFSLRANAYWHDGRPVTTQDVVYTMGLLREENELIPPDLRAFWSEIQVTALSDTIV
                     EFALPEAFSPLLDYLSVPLLPSHLLGNLTLAELVDHPFNTAPIGTGPYKFNRFLVKDG
                     LVSGVDLLANQDYYLGRPYIDEVVFQYYPSMRAAWTAFQGGEVDGLGGVSNDILPEVL
                     AEPGLNLFSTREPRLSMVFLNLNNSAKDFLQDPEFRQALMLSINRQAILDKVLLGQGV
                     LAHGPILPGSWAYYADQGSYSFDPDLAAQKIAALGITRSEAGTLTTAEGVEVRIILLT
                     PDDELHMQIADSIKQGWESVGVAVDVLSMPYADVIARLDARDFDAALVEIDLSGTPDP
                     DPYPFWAQSQAQSGQNYSQWNNRSASEYLEQARVSNDYNLRTKLYRNFQILFHDQLPS
                     LPLFYPVYNYAIRDTILNVTIGPIYEASDRLNAINTWFILAGNAPEGE"
     gene            20585..21112
                     /locus_tag="PKL60_00325"
     CDS             20585..21112
                     /locus_tag="PKL60_00325"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HNW94610.1"
                     /translation="MAARRALARKPGCTSLIREENVSKADPRLRAIGAIDEASASLSL
                     AKAFLPGEPEKAALETCQEHLSLIMAGLAGWKQADFPESAARALDWLEALLTEMENSV
                     ELPGGFLHPGKSPAEGALDLARAVVRRAERETVALAQSESGAELPADALTYLNRLSSL
                     CFLLLAAQIAGQKPE"
     gene            21129..21848
                     /locus_tag="PKL60_00330"
     CDS             21129..21848
                     /locus_tag="PKL60_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNW94611.1"
                     /translation="MIGTFINVAAILLGGLLGLTAGNKIPAKFRQTIVSGLGLFTLAY
                     GVYLFTETRNMLIPLGALILGTIVGEWLRLEERLENLGAFLQTKFNHAEGSGSADAQR
                     FINGFVAASLLFCIGPMAILGSIQDGLSGDFQMLAIKSLLDGLTSVAFASSLGVGVLF
                     SALPVLVYQGGISLLARALGQGFDASVVAEMTAVGGVILTGIAISSLLEIKKIRTGSF
                     LPALVFAVLIVLGLNAMGVSY"
     gene            complement(21929..22594)
                     /locus_tag="PKL60_00335"
     CDS             complement(21929..22594)
                     /locus_tag="PKL60_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNW94612.1"
                     /translation="MPGTQSQREKLREQRKQASKKKTVTLLIVLGAAALAVFLLVMLP
                     KWTLDKDQLYMQDGFSIGDPNAPVKVEEFSDFRCSHCQNFALNYEADFIEKYVDTGKV
                     YLTFHNYAFLAEDSADAAEATYCAAEQNAMWQYKKLLFTYSTYTGAFAEANLIDYAGQ
                     LDLDTDKFSACLQSDANLAKLDEIMSYANSLGIDSTPQFSVNGTIVYMDTLDETVDAA
                     LLR"
     gene            complement(22664..23515)
                     /locus_tag="PKL60_00340"
     CDS             complement(22664..23515)
                     /locus_tag="PKL60_00340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW94613.1"
                     /translation="MVPLILPASAYPKSALVSFLNSAPLSFSNLDWISDRERLEGQFC
                     YTLIDESQVKALISCEPENHSAAWLRFFLSQSDGEHSGYFTNLLERALADLRAARVPA
                     LFTLSFKSWHEQLFSDHGFAGYTQIITLIRTGVLTPPVKPHPRLSIRPITADDLPEIE
                     ELDHAAFSPPWQLNTKTLQAAFRACSYSTAAFLEDKLVGYQMTAASFDSAHLARLAVL
                     PQAQQQGVGSGLALDTIREAESRGSTDFTVNTQTNNAASLKLYKRLGFRQEGRLIPVL
                     IRAVSAS"
     gene            complement(23578..25170)
                     /locus_tag="PKL60_00345"
     CDS             complement(23578..25170)
                     /locus_tag="PKL60_00345"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNW94614.1"
                     /translation="MTERVLLKNGYFYQGSGSQTLLQSALVEDGVISALAFPEEFSSG
                     LRHSCRQIDLQGQTVWPGLVDSHLHLAFLAEQLAAVDCETGSLAECLARVEAKSRGLS
                     QGEWVIGYGWNQNAWEPANYGTAAELEQVSAGHPAVLYAKSLHASWANETALRAAGID
                     ISSADPAGGRILRDSQGKPTGILLENAVQLVDAVVPPLAGKRLESMLKHAQALLLSYG
                     LTSVHDFDRWDCFQALLALDAAGELLLRVRKNLHAERLADIAAEGLRSGSGTEHLFLG
                     SLKYFADGALGPQTAAMLSPYENSDSAGILLMDADEVYAQGLNAAAVGFPLAIHAIGD
                     LAVRAVLDGYQRLRAWERENGRPPLPHRIEHVQLITPEDQPRLKALDVIASMQPIHAP
                     SDMFTADQHWGSRSVNAYAFSALLKQGTQLIFGSDAPVESANPFLGLHAAVTRRRVSG
                     EPGAQGWYPEQRISLAQALDAYTGNPARSCGLGQSFGRIASGYRADLIVLPRDPFRMN
                     PQDLWQLAPEMTLVDGRVVFQR"
     gene            complement(25163..26032)
                     /locus_tag="PKL60_00350"
     CDS             complement(25163..26032)
                     /locus_tag="PKL60_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNW94615.1"
                     /translation="MARKTGLGKGLDALIPTWQQESYSSLPANQVVQIAIDKITPNPR
                     QPRKAFSAESLQELANSIQEHGILQPLILVQNEGEERYTLIAGERRWRAASLAGLQDV
                     PAIVRSATLQEQLEFAIVENVQREDLNVLERAHAYQSLLNEFALTHEDIAQKVGKSRA
                     AVTNTLRLLNLPASVQQALQDDLITEGHARPLLALNSTRAIEAALETILNLGLNVRQT
                     ELLITKLLGKAPRKKIEPGKSAELQALENKLRQFFNTKVNLQSGKKGGSITIYYYSDE
                     ELNGITDALGLND"
     gene            complement(26037..26828)
                     /locus_tag="PKL60_00355"
     CDS             complement(26037..26828)
                     /locus_tag="PKL60_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNW94616.1"
                     /translation="MGKVYTIVNQKGGVGKTTTSINLGAYLGAFQQRVLLVDIDPQAN
                     ATSCLGVDKNSVQNGIYEALIGDQPAADHILHNARFHLSLLPSSPNLAGAEIELIDLP
                     ERETRLRKLIDPLRSRYDYILIDCPPSLGLLTLNGLIAAFDGVIIPVQCEYLALEGLG
                     QLTQTLGRVRQGLFPTLDIKGVVLTMFDGRTNLSADVMSEVQRVFPNRVFNTFIPRSI
                     RLAEAPSFGMPISVYAPESNGAKAYRALAMELLSSNGVHIPEVQE"
     gene            complement(26838..27686)
                     /locus_tag="PKL60_00360"
     CDS             complement(26838..27686)
                     /locus_tag="PKL60_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNW94617.1"
                     /translation="MELSTSEPIDYLVIGHITQDLLDDGTYRPGGTVSYSGLTAKALG
                     RRVGILTSCAADTDLDAYAGIQVRTLPAAQSTVFRNIPTDSGRLQYMYHSARRLDASS
                     VPEEWKNSPIVHLGPVAAEIDPDIYTAFPRSLLCLTPQGWLRSFAQDGRVFPVDWTYS
                     PKLLQAAHAVVLSLEDLHNDEKKVEKWASMCHLLAVTESDQGARVYWNGDVRRFPAPK
                     VALAEDTGAGDIFAACFFHRLYSTQDPWEAARFAVRLAAASVTRRHFHSTPTSVEISA
                     ALTQII"
     gene            27841..29601
                     /locus_tag="PKL60_00365"
     CDS             27841..29601
                     /locus_tag="PKL60_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNW94618.1"
                     /translation="MSLLLEYDCMEKDITTYQKALEDFKKARSKAALQRFWAGIRGKS
                     LDLLPYDEISAKLQAFNKVDRGLQTVKLKDIVGSVGRNEDFDRNFLPLNDADIIRWAQ
                     VKTAMTSPTGAGLPPVSLYKVGDAYFVLDGNHRVSIAREMGLDAIEAYVTEIYTRVPV
                     STDITAEDLHIKATYVGFLEKTRLDQILPGVDFSLNQTENYPLLEEHIAVHRYYMGIE
                     QGREIPYEAAVLDWYEHVYQPVVEMIHSSGLREEFKHLTITDLYLWVLDQQSILQEAL
                     GIPIRSAHAAEYMALQEGKTMRPPTSPTEQHFEETVFSDSAKAAFSLPYVQDNPEGCL
                     FRDILVAIGDHDDYWKALEQAVLINLCQVGHVRGLHVISPSDTLDDTAHQMMELRFEK
                     RLAEMGMSGKLLIVSGEITNKLSEHSLLSDLLVLRLAFPPTGGILDRLTSGIGSILHS
                     AKRPVLIVKEQALPFERMMVVYNGSPKSKEALFVGAYFAGRFDTKLCLFTLDDGTPDL
                     ESETSYAKTYLRKLNLDFEFILRKGGNLAEAALEEAAAFGATTIVLGGYSGNSLFDRL
                     FGSTIDNLLQKTELPVLICQ"
     gene            complement(29587..33414)
                     /locus_tag="PKL60_00370"
     CDS             complement(29587..33414)
                     /locus_tag="PKL60_00370"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNW94619.1"
                     /translation="MKTFTGEDQADLWKALTDFCQQSGKHDPPEAYLCSVSKFIQQEL
                     SAQSSFHFPDPSPFPDNECQGLSASLTKIHLQVERSGKRVMDTNVADQSALLGVPLLT
                     RNTVLGSLVVKRELQPFDAVEICFVDALAAAVSMELALLSSEIRQENQKTRLALIASV
                     VSSIAHITRLDELAREVSQAILRAFGFYYVAIFTLERKTNTVLFRASARSENSDQPAF
                     ELPAGTQISLGDHIVGHVAQSGRALLANDVSKEPRYGRLESLPETKAELALPLKAGGS
                     VLGVLDVQSDQPDVFTEEDMVVLSALADSIGLAVHKVRLYSELEERTEQRDVIAGLTE
                     AISSILDLDTLLNKVAQMIHSQLKYPFVHIFLLDRVRNTIDFHSGSGSRAKKYREHKV
                     SFELDAPKGLIPLTVRRNRIHLASNVRRDPEYMPSPIIGAHSGSELCIPLAFNREVFG
                     VLDVQSAKTNAFDAADIDLLSTLSASISIAIRNAKLYNAEKWRSRVAESLQSVARKLT
                     ESSALEEKFDFILESISAVLPCDAAAIWLLDETPRKSGEAPGCLNLAAYKSQRTAEHP
                     HLENCLSGELAWYPEALSRNEPVVRQNSGKPDPLAEALRLPDGFSALSAPLSAGGKQL
                     GVLTLHHATANRYGPESVSISASFARYAGIAVENERLAQESRDQNWISTILLQVAKAT
                     QSLTRINELTDLIGQLITLLIGGKQGAVFLLEPEERSYYLQGVFGEDLKTLNTTLPSR
                     VGRPKVFVQAAEKLEPMAFPAEECEPDLRAMLGLEPEDTILLLPLIAHEELLGFLLHA
                     STDAYQPAPPESVLGRQKYAILQGIAQQVAVSAQNINLLNSRQEETYISSVLLQVSQT
                     IVTTPDLVEAMDRIYYVLHMLIGVEGCATFRWLPEEDSYELKHAASEQLHPWQLERLY
                     GARIPRAEFPFIPGSEDGKPNLVRSDEVRGTYNLLANDALRSESDTDSPLIKASNSVY
                     LIYKLAMRGEDYGVLICRDPDIPKRERRMELLNGVAQQISFAIQNERLEKVKADQQRV
                     EQEFHLARQIQETFLPEKLPEINGYELDVRWQTARQVGGDFYDIFALRNGSYGMVIAD
                     VSDKGIAAALYMTVTRTLIRAVALESDSPAKTLERVNQLLQLDSQRGFFVTCFYGVLD
                     PLNHRLTFANAGHLPPFLLNPKTKTASPLQRGGTALGIYEDAVYVERVLAIEPGCGLV
                     LFTDGVTEAFNKLGSFYGMTRYQKVLQRCAALSPGALLDAVAEDLASFRREVPLSDDI
                     TELVIRRLPLLAD"
     gene            complement(33411..33830)
                     /locus_tag="PKL60_00375"
     CDS             complement(33411..33830)
                     /locus_tag="PKL60_00375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW94620.1"
                     /translation="MSTQVFPANYQALGAINEFILTEASPIGFSPKELYAVELSVNEA
                     AANIIDHSYCGEDLGTIEVTAEASSQGLTITLKDCGAPFDPDQIPDPDISSPLEMRRE
                     RGLGVYTMRKLMDEVFFDFSQPGVNTLVMTKRKKTQP"
     gene            33909..34613
                     /gene="radC"
                     /locus_tag="PKL60_00380"
     CDS             33909..34613
                     /gene="radC"
                     /locus_tag="PKL60_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNW94621.1"
                     /translation="MTKESGRPTIKEMREENRPRERLIAEGPQALSDGELLAVLLGSG
                     KAGLNAIELGNQILMELGGFSGIHRTDISELENISGVGLAKAARIKAAVELGNRLAKE
                     RTEAKTVIRSPEDVVRLVGLEMRGKEQEELWVIWLDVRNQVLGIDRLYKGSQDSSSVR
                     VGEIFTRAIRKGANAVILVHNHPSGDASESPEDVNLTRAVIEAGRLLDMRVHDHIIIG
                     RDDFNSIKKNHPALWV"
     gene            complement(34610..35302)
                     /locus_tag="PKL60_00385"
     CDS             complement(34610..35302)
                     /locus_tag="PKL60_00385"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNW94622.1"
                     /translation="MNTPPDISDPNAWILYADADLLVVNKPAGLLSIPDGYHPGLPHL
                     AQALSPAVGKLWIVHRLDKETSGVMLLARSAEAHRQLNQSFRERALRKLYHCLAAPNP
                     QWQSQDLDPPLRVNADRAHRTRVDLKRGKPARTLVNVLQRGESFALLECALFSGYTHQ
                     IRAHLYAAGLGILGDALYHPKALNTEDARLQAERMMLHAYEIGFKHPSSGLELSFRAP
                     YPQDFEKKLSSL"
     gene            35370..35678
                     /locus_tag="PKL60_00390"
     CDS             35370..35678
                     /locus_tag="PKL60_00390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNW94623.1"
                     /translation="MFTVIVTIQVVPECVQDFISITRYNASNSRLEAGVVRFDFFQEL
                     DRPESFRLVEIYREQADQAAHQQTEHYLKWREAAAPMMAAERTRVFLRNLDPTDAEWL
                     "
     gene            complement(35808..36653)
                     /locus_tag="PKL60_00395"
     CDS             complement(35808..36653)
                     /locus_tag="PKL60_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNW94624.1"
                     /translation="MIQIVVDTTCGIPLTRLSAQGIHVLPQIITFGDVSYRDDTEIDT
                     ETFLTKLAASKQLPGTAAPPPAMYAPTFQKILDAGDTALVITPSDKMSGTYRSALVAA
                     EEFHSDRIHILNTKTIAGGLGSLVLQASCWAKQGMEIETLKSKLSAMAARENVYFVVD
                     TLEYLHKGGRIGGASRLVGSVLQIKPILTIKDGQVEPFDKVRTLKQALATIVDLDRAI
                     LRNNPNGHLTISQCAAEEQAIQLKAQLEKELHLTDIPIFVVPPAIVVHVGPGVVTTSC
                     FSAPE"
     gene            complement(36772..37359)
                     /locus_tag="PKL60_00400"
     CDS             complement(36772..37359)
                     /locus_tag="PKL60_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNW94625.1"
                     /translation="MTPRIYHGSFSPEDIARDLISYFHRGNYQVQRLGGPDNMAVQIA
                     TRRNLSSGGATALTVSLQKVADGVSVQLSNQAWFGLAASLGMTALNALRNPWTLIGRM
                     DDIAQDVESLQLQENVWQVVDSFARQHGTGQELSERLARTVCPYCLTANPIASARCLA
                     CGAPLGENQASTCAKCGFILEKKEKICPNCGQPRS"
     gene            37423..38055
                     /locus_tag="PKL60_00405"
     CDS             37423..38055
                     /locus_tag="PKL60_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNW94626.1"
                     /translation="MNFLKKHRLPWLFSVLILLLLTALSLLPALENPVLAQFPTVSIP
                     TVTSSPRGAYIRVLLTTTEYDPINVRSGPGSVYELVGILLVGQEAKAYGYYDQYIQIE
                     YPGGPGGRGWVFANNVDVIGGPIPLLEPPATATPAVTKTIDPTLAAQFIYTSQPSRLP
                     TFTEPPALNLPTYQNITGNTAPGGLPLGLVIVVLAGLGVFLTLIAILRRG"
     gene            38169..38618
                     /gene="smpB"
                     /locus_tag="PKL60_00410"
     CDS             38169..38618
                     /gene="smpB"
                     /locus_tag="PKL60_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNW94627.1"
                     /translation="MDVKTVSTNRKARFEYFLQETFEAGLELKGTEIKSIRKGQISLQ
                     EAYVRTDGKQAWLVGAHVAPYEHASAYQHDPDRERRLLLHKREIRELWDAVRIKGMTI
                     VPVRVYLKAGRAKLELAIAKGKKQYDKREAIKQKDIEREESRDERRY"
     gene            38676..39752
                     /locus_tag="PKL60_00415"
     CDS             38676..39752
                     /locus_tag="PKL60_00415"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNW94628.1"
                     /translation="MKPDNKITRREFLKLSGMFIGAMMLPRPKGVFAAAARQFPQADK
                     LGRICVGMEGAWFKLKTEPNVNAPEAGTVWRDDVLVWSREVVANQLDLNVYNQRWVET
                     PGGYLQSSMVQPVKNLPNQPLQSLPVNPDGSQGMWVEITVPLVDIKPTRVPAASFWIR
                     EVNKPRIYYSQVFWAYDVRQENGITEYLLTEKWGAEPDSYWVDASACRPITADEITPI
                     HPDAGDKLIRVNMNYQTLSCLEGGREVYFCEVSSGGPSTPLGTTLIWRKMISTHMSAG
                     GLIAYDTGGIGWTTLFHGEGSAIHAAFWHNNFGTALSHGCINCLPEDAKWIWRWTNPV
                     VDYYPGELTVSDGGQNSTRVEVFY"
     gene            39808..40833
                     /locus_tag="PKL60_00420"
     CDS             39808..40833
                     /locus_tag="PKL60_00420"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNW94629.1"
                     /translation="MNDEVIQVTLYSRPSLAEQNEAVLERLAELAQEYPHQLTCIDID
                     RNNYLKRSIGEQIPRLEIGPYFLIESFDSGDIRTALKETRAKIDDAHTSANPWVLRQY
                     ARPRSFPLRDRFSLWFARHYVMLFTLLVFLYLGGAFLAPTLMKLKLEPAARALYSFYR
                     PVCHQLGYRSFYLFGGQPFYPRELAGLESYQTFSQATGINELDNRAASEFLGSETLGY
                     KVALCQRDTAIYTLMLLFGLIFGASGGRIKEIRWYIWVLLGILPIALDGGTQMLSQMG
                     LAFLSWFPARESTPFLRVLTGGLFGLFTAWLGYPLVESSVQISRPQLERQYALNSGAA
                     FEPGRRP"
     gene            40830..41624
                     /gene="pgeF"
                     /locus_tag="PKL60_00425"
     CDS             40830..41624
                     /gene="pgeF"
                     /locus_tag="PKL60_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNW94630.1"
                     /translation="MIRCDVDGCRYYRFEQLPNQQVDHAVFTRLGGASAGPYAALNLG
                     GTVGDKPEAVLENHQRLFQVFGRPYESRFDVWQVHGTTMVYSDQPRPRDQKHRPADGI
                     FTDNPEVTLIMRFADCVPLVFFDPTHHVVGLVHAGWQGTAQKIGELAVNYMRERFNSD
                     PAALFAGIGPSICGSCYQTGEEVRARFLQAYGADALDFFMEREGSLYLDLWRANAYCL
                     QRAGVQQLEQCGLCTAEDLDQWYSYRKEKGITGRFAVVIGLKHAAQ"
     gene            41611..42363
                     /locus_tag="PKL60_00430"
     CDS             41611..42363
                     /locus_tag="PKL60_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNW94631.1"
                     /translation="MLHSEDESRFIRTIGARLALVRENIARSAAKAGRRAEAVEIVTI
                     SKRQPEVYIQAAYACGLRCFGENYAEEALVKMNNLSGLPDLQWDIVGHIQSRKARLIA
                     DRVARVHSVDSLKLAGLLSQLRPADMPALDVLLEVNVSGEESKGGFPAVSQADWSALL
                     PAADAVSRLPRLRLRGLMCMPPLQTEMENNRSYFSKTRELLDFLKAALPGLELSELSM
                     GTSSDYPVAVEEGATLVRLGEAILGPRPVKAE"
     gene            42370..42633
                     /locus_tag="PKL60_00435"
     CDS             42370..42633
                     /locus_tag="PKL60_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNW94632.1"
                     /translation="MTIALTIIHTIGQALSLLVILSTVLSYFLAWDHPVRRGLDKIVT
                     PMLRPIRSLIKPVGGLDFSPFVLILLIYMLEQLLSSLVRALFR"
     gene            complement(42640..43341)
                     /locus_tag="PKL60_00440"
     CDS             complement(42640..43341)
                     /locus_tag="PKL60_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNW94633.1"
                     /translation="MNLTFSLFRLQTLDTQRQKITKRLAQIEAILNADEAVQASLREK
                     TQAEADLTARQQAVNQIAAETAEKRLKLELNQVQLFGGKIRNPKELQDLQAESEALRR
                     YIQKLEESQFEALMAQETAQKTLVEAETAYLAALNQKAAENSLLAGERGTLLTEMSNL
                     NSQREALTQTLPADIVAQYENLLKTKGGKAVAAIVDDGCEACGLELSPRDMQAVRSAT
                     TLLRCKTCGRILYKP"
     gene            43671..44747
                     /locus_tag="PKL60_00445"
     CDS             43671..44747
                     /locus_tag="PKL60_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNW94634.1"
                     /translation="MAFNPINWLLGLFSLDIGIDLGTANTLVNVKGKGIVINEPSWVA
                     IDKRTREPLKIGAAAKAMVGRAPANVVTLRPLRDGVISEYDITQAMLEFFIGRAHQHS
                     IVPFPRPRVVVGIPSGATEVEKRAVYDAAMAAGAREAYMIHEPAAAAIGAGLPVNDGN
                     GNMIVDIGGGTTEVAVISMGGIVISRSLRVAGDELDQDIIEYLRSKYNLFIGEQMAEK
                     VKIAIGSAYPLKDEKTVDVRGRNLITGLPEAIEVSSVEIREALSASVQVIIDTIKDAL
                     DESPPEILSDLMDNGICMAGGMSQLQGLAERVSNEVRVRVWVAEDPMTCVARGAGIVL
                     DDLEEYEQLLVGIERDPYTSLTKG"
     gene            44783..45619
                     /gene="mreC"
                     /locus_tag="PKL60_00450"
     CDS             44783..45619
                     /gene="mreC"
                     /locus_tag="PKL60_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNW94635.1"
                     /translation="MRNLFTSGNIRNFIILLVVAGILLMAVSGYLTPLFSLTFSPLIS
                     TQSWLSQRYLAIRDFFTSPRDMASLRERNAQLENEVSQLQSQIVELQENLSQAQILYT
                     LLDFARTNPQHEYTAATVIGREISPYYQYIIIDKGSNQGIMHGMPVVTQQGLVGRIDA
                     VIANASRVQLISDAESVVNVHLQNAKVEGQIVGSITGEITLEMIPQDAEVQIGDVLLT
                     SGLGGNYPPNIFVGQVLSMQSQQNRLFLSGSVQSIVDFSSLSAVLIITNFEAVDITPL
                     VP"
     gene            45628..46131
                     /locus_tag="PKL60_00455"
     CDS             45628..46131
                     /locus_tag="PKL60_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94636.1"
                     /translation="MFFYISSSIGFLIAIMLQMGVISRAALLSGTADVVLLFLAAWSL
                     HQRQKYSWMLVVIFGLVLGAISASPTLLFLIVYLSVFLAATRLQMSFWHSPLLSMFLL
                     VFFGTFLEHGVLILGLFVQGVSINLIEIFTQVLLPSVLLNMLLTIPIHALVQEAFRNA
                     APAGTEI"
     gene            46128..48203
                     /gene="mrdA"
                     /locus_tag="PKL60_00460"
     CDS             46128..48203
                     /gene="mrdA"
                     /locus_tag="PKL60_00460"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNW94637.1"
                     /translation="MNNTHFEKPTINRSRIRGVIFLVAIVVFYYIFQLFNYQIVNGAL
                     YRAQAEDNRTSEISDPTQRGIIYDRNGVVLARNEPAYNITVTPANLPESDGELRKIYS
                     ELSELLKIPVSNGVVDAVTASSFTPCNTTLGIEQIVYIADTNWPFSATRLSCDVSKEI
                     AMAIKEKAMDWPGIDVEIESIRVYPTGNLTAEIVGFLGPVPESMVDYYTGLGFVAGRD
                     KVGYAGVENTMQDVLGGTNGKRVVEVTVGGEVVRNLEEPVDPVPGQNIYLTIDTRLQS
                     AARDALIMNLDYWNRYLGYTLSTSGSVVALNAKTGEVLAMVSYPNYENNRMSKVIPGY
                     YYNQLTLDQAKPLVNQAISAELPPGSVFKLVSALGILNEGVVTPEYTVDDPGTISLVQ
                     RFYQNDPGSLQTYYCWDRAGHGPVDYLHGIAWSCNVYWYKVTGGYGTEIPGNGLGIWR
                     LGTYARALGYGALTGIELPGETDGLIPDPTWKRLSQGENWATGDTYLAAVGQGYVLAT
                     PVQVVHSIATIANDGKLMKLSIISKVEAADGTVTRQFEPTMLWDITKDPKIEVYDDNI
                     PTGELKTVQPWVIELAKKGMEMVTQPGGTAALEFKDDTNKVAGKTGTAEYCDDFALAN
                     RLCGVGIGGWPAHAWFVGYAPYDDPEIVVVAFAYNGKEGSTLAAPIVRKVIESYFSLK
                     AADADNPGY"
     gene            48217..48888
                     /locus_tag="PKL60_00465"
     CDS             48217..48888
                     /locus_tag="PKL60_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNW94638.1"
                     /translation="MSKRTELLVRGIRGGVLVILPDAPWYQQRDMLISRIQSQERFFK
                     GGRIALDLGGTDWSEEHLDKLLRDLADEGICLRAVLSSSQITLVSAQVFGLPPSIEGD
                     ERPKAAAPEAEAGPRVVWIDRPLENAEKLIVDGNLALFSDLPAGAEVICSGSAALWGC
                     LDGILRAGCAGDPDSAVNILKLGLGSVSLNGEAVKIPRKLHKTVGIMIRRQDGAINVV
                     PLVKK"
     gene            48894..50009
                     /locus_tag="PKL60_00470"
     CDS             48894..50009
                     /locus_tag="PKL60_00470"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNW94639.1"
                     /translation="MFTTRQQWQHFDFPLFAAVLFLSIFGVALISSAIAGNPTLANHP
                     QRQIVFLIIGLAALFIFAGLDYKIWLVLTRPFYIVVALFLILVFVSGAARFGAARWLT
                     IGSEAIQPAELAKIVIIMALAQFFASKISSINEWKTVGQSLLLTGGIVIWIILQPNLS
                     TSIVIMVIWFAMLWISGWKTKNILILAGVAIVLLGLFAALDFPFLADYQKARITNFIN
                     PDENARYGDTYNVDQALISIGSGSWFGQGYGQGSQVQLRFLKIRHNDFIFSVLAHEFG
                     FVGTVAVLLLLLFVIFRCFQIAARAPDAYGALIAYGFGVLMAFQTLVNVGVNLRLLPV
                     TGLTLPFISYGGSSLVTLLMGIGLVQSVRMRSEQRPG"
     gene            50066..50136
                     /locus_tag="PKL60_00475"
     tRNA            50066..50136
                     /locus_tag="PKL60_00475"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50098..50100,aa:Gln,seq:ctg)
BASE COUNT        10739 a        13793 c        14922 g        11383 t
ORIGIN      
        1 ggttccgggt ttgccctgac gggtacaggc aaatcccagc ctcgccatgt tttcggttaa
       61 tgcgcaaaaa agacttgata ccagtttccc ctactctgcg ctctgcggac tgctcagatt
      121 ccagcctctc ttcagataaa tggtcagaag cggttttttt ctggttaacc ctttccttta
      181 gcctgcagtc aaaagctatt ccagtgaaac tatcgtcacg ctgcttgccc ggggtgcctg
      241 gcatcgtaat cacgccagaa ctcgtcgcat tttttcttcc aatcctcagg gcgtagatca
      301 cccccgaact ttttgccggc tgtagccagg gatatcttac tgatgtaatc gcgataggca
      361 tcaactgacc ggtctttagg ttgcattaaa taagcaatat caattctccg tttcatcgga
      421 aaccaccttt ttgtgtttta ccagccatcg tcaagaatct cgaagggatc caataccaca
      481 cttacgaaga cattaacatg agagcgtaaa cgatttggaa aaacggttag cagacctatt
      541 catagtatag tgaaattcaa atggagaaca aagcaatttt ccagcactgc gccggaggga
      601 aggacaaaac ctcaggcact ccttcagaat tctcgtccgc cattcgcctg ggaaggtctc
      661 tgcgctgaac tggtgtgggg tgtgcattgc caatcctttc ggaattctta ccgcgcaaag
      721 ctgttgggca gcttcaggct agcgtttcgc gatgcagagt tgggattttg cccggtccgc
      781 cccgcaaacg ccaggaagag aaaaacgttt tctttcgaac agttcaagag gttgcgctgt
      841 atcggatgaa atattcaagc tttcatgcag accaggcgat aatatacctc cgctcaataa
      901 tcaaatgtgt ccgtggatta caatgctgga ctgcgaagcc agaattcgcc ggaggttatg
      961 gaaatcctcg gggaaaaaca cctttacatt cgcctgtcga tggattttcg aaggaccggg
     1021 agccgcgctt cagggataaa ctcaagcccg cgcccttcta agccggccag agcgcagcga
     1081 tgtgtcggaa caccagttga caatctgttg aacagcttgt attcccataa aaaacctttt
     1141 gacaaaataa aaaaactgta gtaatatttc aaagttgtat atggtagttt gctattcaaa
     1201 gtgcttgtca tccggcagta aacccccatt tatcaaatca cacttttttt atatatagag
     1261 aggatcagta cataatgtca gaacgtgaag ttggaaccgt caaatggttc aatagtgaaa
     1321 aaggttttgg gttcatagcc cgcaattccg gcggtaaaga cgtcttcgtg cactacagcg
     1381 ccatcatttc cgatggtttc aagaccctgc aggaaaatca gtctgttgaa ttcaccgttg
     1441 cccagggcgc aaaaggcccc caggcgcagg atgtaaaggt cctctagacc tccataacca
     1501 agattcccaa agggctgacc gtcaggtcag ctttttgttt taacccgata ggaagctgcc
     1561 cttctctttc accgtttcct ttcccattgc tccaatcccg agccctgcgt gcgttctgct
     1621 cacaatccgc ttcgcttaca tcccaaaccg cgccgttaag tacgctaagg cacgcctgga
     1681 atatccctat gttctattat cgctcaacct gcgcttgaaa atcaataaat cagaatgtat
     1741 agcaactatc gtccaccgtc tgggatttgg attccgataa acacaacacg tctatccact
     1801 gtcgcatttt gtgtttcaat tctgttgact ttgtttttgc tgctgagcgt cgtcaagctt
     1861 gcttctgctg atccggaaga gaaagtgagc atttgccatg ttacaggaaa tggcaatgag
     1921 attaccctga cgctcggtga ggcggcgctg aaaggtcatt tcaatgggga tggcacccct
     1981 cgcgaagggg gtttgcgcga ctattgggcc gattgcggta ggacagaatc agcacaaaat
     2041 ttcatcgcat gccgcttagg tcccacaagc ttaaagcgca ttgatactcc atagcgccga
     2101 cgcatgccgt ttgcacacag gcaagggaca gcccttcctg gcagcgaagg ctcgggcggc
     2161 gccagacttc cacgattgag gagcgtcttt gggggctgcc cggcatgcct tgctttcgtg
     2221 ttccgatgat ggcccgggct tggtattaac aatccaggtg ttgatcatcc gtaggcagcc
     2281 aaagacactg gccggcatgc tggcctatcg ctttttccgc gttgggtcag ctgccaacat
     2341 gaggaagctc acacgccgcc caaccccgga tgaaccagct ccggggaact tgatcgggtt
     2401 tctgaagaaa agggagcagt catgccaggc gcagctggaa atcgaatgat tcagagtagg
     2461 ttttcagacc ctggaatgtt acatcatcgc aggcgggcgc tccagttttc agcccggctt
     2521 gggatgatat aatctaatca ccacaaattc tgaggtgatt atgtccgata cgattacttt
     2581 gcaggaaatt gattctgcgg ccgcgtgcgt gcgcggtcgg atttccatcc agccccaaat
     2641 cgcgctcatt ctgggctccg ggttgggccc tttggcggat gaagttgaaa gccctgcgat
     2701 tatacccact aacgaaatcc cgaactggcc tgtctctacc gtccaggggc acaaaggacg
     2761 tctggtgatc ggccggctga tgggaaaacc cattctcgtg cttcaggggc gcgcgcatta
     2821 ctacgaaggc tacagcatgc cgcgtatcgg gctgcctgtg cgcgtgatgc agcgcctggg
     2881 cgttaaaacc gtcgtgctga cgaacgcggc cggcggtatc aatccggatt tttacgccgg
     2941 tgacctgatg ttgattacgg accatctctc ccttttgccc atgaccggac ctaatcccct
     3001 gcgcggaccc aacctggatg aattcggccc ccgctttccg gatatgagcc aggtatacga
     3061 taaagagctg ctcaggctcg cgcaggaggt ttccgacgcg cagcagataa atgtgcggcg
     3121 cggtgtgtat gccgggctgt ccggcccttc gttcgaaact ccggcggact gccgtttcct
     3181 gcgcctggct ggcgcggacg cggtggggat gtccacggtg cctgaggcta tcgtcgcgcg
     3241 gcacgggaat ttgcgcgtat tgggtatttc cgggatttca aacaaaatcg atatcaacgg
     3301 gaacaacgtc gcttcgcatg aggaagtgtt ggaagccggg aaagtcatcg tgccaaagct
     3361 cagcgcgctt atcaaaggcg tacttgccag gttgtagcct tgggtctcgc ggaaaccaca
     3421 gcgcggcaac ctgattgtca gcaatataaa tggatgcaat cgtaaaattc ctcgcggacc
     3481 tggaagtccc aatttatctg attctcggcg tcgtcgcggt gatttatttg cgcaggctcg
     3541 cgcttgcgct ggacgaacgc aaaagctcta ttttcggtct ggagcgcgaa gccgcccaga
     3601 caaaagtcgt ctccgccgcg accgtgatga ttctggtggc gctgctcgcc gtgggtgaat
     3661 ttatcgtggc aacttttttg gccggagaac tggcgcagca gccgctttac gccacgccga
     3721 ccatcgcggt gctgacgacc ccgacaacca cgctgctgcc tgctgccgtg cccaccgatg
     3781 ccacgccgac gccgacgcct tacccgcagg cacaaattga ggggattgag agcaactgcc
     3841 aggcagaggt tctggagatt tctgaaccca aacatgggga tgaggtcagc ggcgtggtgg
     3901 aattgatagg ctctgtgaat actccgaatt ttggttccta tgagtacgag tattccgtga
     3961 tggggcagcc aaactggcag acgattgcag ccggcagcgg cacgcgcgtg cagtccagtc
     4021 tgggtttctg gtacacgacc gacctcgagc cgggagatta cctgctcagg ctggtcgcgc
     4081 tggataacca gggcgaaaga acagcggcat gcgtgattgt cgtcaaggtt agcgcgccgc
     4141 aggaatagag gaaacgatta gcatggcaga attggaaata aaggcagctc ggcaggcgga
     4201 tttggaccgc atgcttgggt taaaccatca ctcgagcagc gaatatgcct ataaattaga
     4261 gctgaccaac gaaaaaggta tttttacgca cagttttcag cgagtcttct taccccgcgc
     4321 ggtcaatatc aaatacccca aagatgaaac agcgctgatt aattcctggg gcgaagccag
     4381 cgcgattttt gtcgggttgg tgctgggcga gctgattgct tatgtggcgc tcgaaacggg
     4441 gcgcattaaa gataccgcgc gcgttaccga tttggtcgtc gcaccggtca tgcgccgcaa
     4501 gggcgttggc agcggcattc ttttggcttg cgaaggctgg gcgcacgacc agaaggcccg
     4561 ccgaattctc atggaaatcc agatgcgcaa cgatcccgcc atccagatgg tgcaaaagct
     4621 gggctatcag atgtgcggct atctggacca gtacttcccg aatcacgata cggctttgtt
     4681 cttcgacaaa cctataagct gatgatatta tcgcttctca caggcgcacc tatgcttacg
     4741 cttactggca tcctgaaaat aatcgcgcaa atcctgaatg cgggcgtggc catcaccgcc
     4801 attgcgctgg cgatgtatgc cctcagcttc aacttccgcg ataacaccgc ccggactttc
     4861 atgattcttt tggcgctggt ggcagtcgtt ttcagcagcg atacgattgc caacatctcc
     4921 aacagccccg cgattattca ggcctggctg cagctgcgct gggcggctat ggcttttctg
     4981 ccggcagcct acttctactt ctccgatgcc ctgctgactc ttaccggccg acccagccgc
     5041 ggaaggcgca cgtggctggt gcgcatcttt atgctcattt ccctggcgtg ggcggtgctc
     5101 atcccgctgc gcatcaccgt aggcccttta gcagaccagg ctaatcctgt ggcatatctg
     5161 caaaccggac cggtttccgt agcatttggt gtgtactatc tggctgtgct tgcgctctca
     5221 ttctacaacc tggcccgggc agtcttacgc agcaatacca ccaccacccg ccggcggctt
     5281 ttttacctgt tcatcggcgc cagcgctcca gcggtcacct gcgtgctcta cctctttcac
     5341 ggcagcgcgc tttttgccta caatccggtt tttttctggg ggctgaccat cattggagcg
     5401 attctcaccg gcatttcgct ggtggtgatt gcttatgtcg tgtccttttt cggattgaat
     5461 tggaccgacc gcgctatcaa gagccgcttg ttccgctggt tgatgcgcgg accttttgtg
     5521 gcgatggtga cgctcggcgc gaccaccgtt gtgcgccgct atgggttgac gctgggcgac
     5581 ccgtacatac cgtatgtgcc tgtcgtgatg gttggaacca tcctgctgtt ggaatacttc
     5641 attaccctgc tcgctccagg ccttgagaaa ccgctgttct ttggcgcgga ccgcgaagac
     5701 ctgagcctta tccgcacgct ggaagaacgc atgctgaccg aaaaagacct ggaccagttc
     5761 ctggaaatca ttgcggcttc catttgcgac caactgcagg tgcgcggcgc gttcattgcc
     5821 gtggtggaaa acggcacggt agactatatt attcacgctg gcgaccggca catcctggat
     5881 aagctgcctg aaacggacac tctcctccgc gcggtacagg ctgagcggaa ggacccgcgg
     5941 gaaatgctca cttttgagtc cttcaggctt atccctttgg cttatcagca ggatatcgag
     6001 caaacccgcc tttttggggt atgcggcttt ccagctgaag acacggatac gtttttccct
     6061 gagcagctga gcgcggtgag gcttttagca gaccgcgcca cgctggcgct gaaagaccgc
     6121 gcgctgcagc tgcaggtgct gaactcgctt tctgtgctgc agccggaggt agattatatc
     6181 caggaactta ttgccagctc cagctataac cagaagggca tttatcgcaa cggcagcacg
     6241 caggtgcccg gcgaattaac ggattgggtc aaggacgcgc ttacgcatta ttggggcggc
     6301 cccaaattga cgaacaaccc gctcctgaat ttacgtctgg ttgaaaatgc cagcgaaaac
     6361 tacgacggca gcaaggccaa cgcgctgcgg gcagtcctga aacaggcaat agagcatatc
     6421 aagccggaag gggaacgcaa attcaccagc gactggattc tttacaatat tctggacctg
     6481 aaatttatcc agggggaaaa agtgcgcgag gtggcgcgca aattggcgat gtccgaagcc
     6541 gacctctacc gcaagcagcg cgtagcgctg gaaagcgtcg ccagaattat catgcgccag
     6601 gaagccgaaa cagctgccgg ggaaaaacaa ccggaaactg actcagaagc tcctgcaagc
     6661 gcttccgggg acgcactctg aggcatagaa attgcaacct tttttccctg gaaagcagac
     6721 attcttgcct ggagttaacg cgtgagccct acaaaagcaa aagcatctga gacaaaaaat
     6781 ggcggcagcc ccgcgcgcga acacagccca tctgccgcgc aaagccccgc gccaaaaccc
     6841 gggaataaag gatggttcga cccttccagt attggttggg atttggttgg ggtgctgttg
     6901 ctggcgcttg gttttgtact gctgctggct gtcttgggtt tcactcgcgg cacaatcctg
     6961 gacctgatcc ttggggctgc ccggcgttgg ttcggtttgg gcaggtacct gctgccgctg
     7021 gcgtttttgg cgggcgggtg gctgctgctt ctgtggcgta aaaacgagca cagttccgtc
     7081 tccgccggtc gggtcgttct ggtggagctt ggactgcttt ttctgttggg cgcgctttcc
     7141 gcgtttacca gagaaactgt gttggaaatt gaagcgggca ccagcctggg cggcagcaca
     7201 ggttgggggc tggctcaccc gctcgcaggt ctggtcggtt cgtttggcgc gggcatcctg
     7261 cttgggctgc tcagcctgct gttcctgctt tatggcttga acttttttag acgcatcgaa
     7321 gcctgggcgc gcgctcgggt gggcgcagcg ccgtctgcct ctgaaacgcc cgcgcccgtg
     7381 ctcgagacaa gcggccgcac ccgcacggcg gagctgcccc gtcagacgcc cgcgaaagag
     7441 gttaagactc agcaaacact gccattgacc taccagaaag tgacgaaaga gactattccg
     7501 gaaaaaccgc cagaagctcc gctcagccga cccgatgtgc ttccaccgct atccctgttg
     7561 gaaacagaga agcctgctgt ggtcaacgaa gcagccattc agatgaacgc cgccatcctg
     7621 gagagaacgc tgggtgaatt tggcattccc gccaaagtcg tcggacaccg cgtcgggccg
     7681 gcgattaccc aatttgcgct ggaacctggc tatatcgaaa agaacggcag cccggaagaa
     7741 cgtcaaaaag tgcgcgtctc caagatttcc aacctctcgc gggatttagc cctcgcgctg
     7801 aaagcggaac ggctgcgcat agaggcgcca gtgccgggga aatcctatgt gggcatcgag
     7861 gtgccgaacg ctgtgcatac gatcgtaaag ctgcggccca ttctggaatc ggaacagttc
     7921 cagcgggtaa acgcccccct ggcattcccg ctggggaaag atgtttccgg cgcgcccata
     7981 gtggctgacc tggctgtgat gccgcatttg ctcattgctg gcaccaccaa ttccggcaaa
     8041 tccatttgca tcgccgcgct tactgtctgc ctggtgatga acaacaaacc cgaaaatttg
     8101 cgcctggtga tgattgaccc gaagcgcgtg gaactgaccc gctttaacgg cttgccgcat
     8161 ttgttaggtc cggtggagac cgacctggag cgcatccaga gcctcctgcg ttgggctgtg
     8221 ggggaaatgg aagcgcgcta caagattttg gaagaagcgc actcccgcaa cctggactcg
     8281 tacaacgcgc gtatgaccca cagcggaaaa ccgaccttac cgcgcattgt gattttgatc
     8341 gacgagctgg cggacctgat gctcgcggct ccggaagaaa cagaccgcgc gctagtgcgc
     8401 ctggcgcaaa aagcgcgcgc cgttggtatt catttggtcg tcgccacgca aaggcccagc
     8461 gtggatgtca tcaccggcag catcaaagcc aacttcccgg ccagaattgg cttcaccgtg
     8521 gcctccctga tggattcgcg cgtgattctg gattctcccg gcgcggaaac cctgctcggt
     8581 aaaggggaca tgcttttcct gaacccgctc accggacagc ttgagcgttc gcagggtgtg
     8641 tacgtaagcg atggggaaat ccgccgcgtc atcgcctggt ggcagcagca gacgggaggc
     8701 gcggaaaaga aaaccgcccc ggcaaaggaa agcccggctg tgaatgaagg gccggcttca
     8761 ccctgggagc agcaagtgag cgaggattcc gaggacagcg acgaaacact ggttaagcaa
     8821 gctgtggaag tgctgcggcg cacaaaaagg gccagcgcgt ctttcttgca aaggcagctg
     8881 cacctcagtt atccccgggc tgcctggttg gtggatgaac tggaaaaccg cggcattatc
     8941 ggaccagcgc aaagcggcgg gaaaaaccgt cagatcctgc tggatgagaa tgctgacgag
     9001 accgatgaag cggttgaata gcctttcgag tgagcgcgca tgaatgaaca gaaaacaaat
     9061 cagaaatcct tcgaattcct gctgcgaaaa actttcagcg gaattttgga tgggattgcg
     9121 ggctttctgc ttggtttggg gttgaaaccc aatcacatca cactggcagg tctttgcggg
     9181 aatgttgtgg cggcgttgct gattggcagc gggtacctgg tatggggggg agtgacagcc
     9241 gccctgatgg gtccgctgga tgcggtggac ggcgccatgg cacgtaaact gggaacgccc
     9301 acccgttttg gcggctttct ggattccgtt atcgaccgct atgatgaact tttgctcttg
     9361 ggcggcgtgc tggtcttttt tacccgcgaa gcgaatacgg ccggtattta cctcacctac
     9421 gccgcggctg ccggctcggt attggtctcg tacatgcgcg cgcgcgccga agccctgggt
     9481 tttgacggaa aagtgggctt actgacccgc gtagaacgtt acatcatttt ggttatcggt
     9541 ctactgttga atttgacgat ttattctgtt ggtataattg ctgtcttagg aaatgcaacc
     9601 gctatccagc gtctttttta tgtcaaaaaa caaaccggcg aaaacacccg ccgggaataa
     9661 ttgcaaagga ctttattatg ccgcaaggat ttcagatagg accgctcaca attcgatatt
     9721 acgccctgat cattctggct ggcgcgcttt taggggcctg gcttgcctcc gtggaggcca
     9781 ggcgccgcga taaagacggc aacctggtat gggatatttt gccctggctg ctagtcggcg
     9841 ggattattgg cgcacggctc tggcatgtgt tcacgccgtc cgcttccaac atcgccatgg
     9901 ggctgaccac caaatactac cttacccatc cgcttcaaat cctgatgatt tggaacggcg
     9961 gactgggaat tccaggtgga atcattggtg gaggactggc gttgttgatt tattgcaagg
    10021 tgaccaaaca aagtttcctc gagtgggcgg atattatcag cccagggctg cttgtcgggc
    10081 aagccgtcgg gcgctggggc aattttgtta accaggaact ctacggcagt ccatccaacc
    10141 ttccctgggc aataaccatt gacccgcttt acaggctgga tggatttaag gaggtttcga
    10201 aataccaccc tctcttcctt tacgagtcgg tcttgaacct gcttgccgct ggaatcctgc
    10261 tctttgtggg gcgaaagtac aaaaacaagc tgtaccgcgg tgatattttc ctgctctatc
    10321 tggtgctata tccggtcata cgctttgggt tggagttctt gcgtctggat ccttctcctg
    10381 tggctgggtt taacattaac cagactgtga tgggcattgt ggccctgctc tctgtcgcgc
    10441 tgttggtcct ccgccatacg atccgccgac caaaagctgt acaggaactc gagactgaag
    10501 gcattgatat ttcggatatg cagacagtcg ccattgaaga agccgggttg gaatccgata
    10561 ccgctgccct gggggatgac gggctggaac cggttcaggg ggatgctggt ctggagggtc
    10621 cgtttgagcc tctgactgac atgacggaag aggcccccca gattgaaccg gaaggttcga
    10681 acgctgagga tgagacccac ttccctgaag attaaataca agctttggta attgaagcct
    10741 ccggaatttc cggaggcttt tttcgaagat tcaccgcacg gaattaaagt gcggtaaaat
    10801 ttaacattac tggcatagcg cggcgcagag aacgcacagg ccatctcggc ggcgtgacgg
    10861 aggcaaaaga tgaacattga tgaacaggtc gaatatctca tgcagggtgc ggactacggc
    10921 gacacggccg tggaacaagc catggcggtg gaactgcgcg aacgcttgat tgaagccggg
    10981 cgcgaaggcc gcccgctgca ggtctattgc ggttacgacc caacctccac cgatttacac
    11041 cttggacata ctgtcaccat gcgcaagctg cgccaatttc aggagctggg gcacgaagtg
    11101 accttcctga ttggcaactt tacctccctt atcggcgacc cttccgacaa agacaagctg
    11161 cgcccgcaga tgagcaccga gaaagtcatg cacaatgctg agacctacgc ggagcaggct
    11221 ttccgcgtgc tcgaccctga gagaaccaag gtgcgttaca actctgaatg gctgtccagg
    11281 ctgacgatgg aggatttgtt tcgtctcgct tccaatttca cagtccagca attcctgacc
    11341 cgcgataact tccgcaagcg ctgggataac gccgagccga tttacctgca cgaatttttc
    11401 tactcgctga tgcagggtta cgacgcgcat cacttgaacg cggatgtgca ggtcggcggg
    11461 tctgaccagt tgttcaacat catgaccgct gcccgcaaac tgatgacttt cttcgggcag
    11521 aaaccgaaca tcggcatcat cctgggcatc ctgcccggca cagacggcga agtgcgcatg
    11581 agcaaatcgc tgggcaacca catcccgctg aacaccagcg ccgaggatat gtacggcaaa
    11641 gtgatgagca tcccggacaa agtcatgccc gtttacgcac gcctggcgac ggattggccg
    11701 ccggaaacgc tgaaccactt cctgactgct ttggaaagca acagcatgca cccccgcgat
    11761 gccaaaatga aactggcgcg cggcattacc gcggtgttct acggggaaga ggcggccgaa
    11821 agcgcgcaaa atcagttcat tgccatgttc cagaagggcg atgccccgga cgagatgcca
    11881 gaataccggc tcgcaggcgc ggagaatttg ttggaagtca tggtgaaaaa caagctggca
    11941 cagagccgaa accaggcgag aaggctggta gagcagcagg gcgtgaaaat agacggcata
    12001 accgtcgggg atgtaaccct gattattgac caaccttgta tcttacaggt cggcaaacgc
    12061 cattacctgc gtatcctgcg ttgagatttc agggcgtcat cagagtcagg agctgacttc
    12121 cgatttgctg gctgagggcc ggttcgctgc cctcaatcgc aaccgcgatt gccagtggga
    12181 cgccgaccca ctgcggggtg ctgccgccga tgtaccaggt tagcgtttcg ccgctttcca
    12241 gtgcggcatg cccgttcaga taccagatag attgggattc cgccaggaac tggttgcgaa
    12301 gctgcagtgc caggtcgcta ttgaagacct ccagcgctga agtgttccca ccaaaagcta
    12361 cccaggcgcc gttttcggac tgatagctgt ttaccaactt cggggtgggc tgcagaccgt
    12421 cattagtgag ggcggctgcc atgagcgcca tttgcagcgg ggtcacattt gtctccgcag
    12481 ccgtttgcag ccacacagcc aaatcatccc ctaaggccgg gtccggagca gcttgcgcgg
    12541 attcgaccgc tacggctggt tcgcggaaga atccgaacgc ccgcatgata tcagccaact
    12601 cttccggcgt tgttccgcgc agcaggtccg cggacgcgct ttcacatccg tattggagca
    12661 cattcacgcg cgaggatgcg gctttcatag cctgataaca gggcaggtcc aggcgtgggg
    12721 agagctcagg gatttgatac cggatttgag ggtcagtcca ggcggctgcc agcgcgggca
    12781 aattcaggag cgtgccaacg ggataagccg cctgcgttgt gcgattcagc atgggagcgt
    12841 cctcgcggct catcaaatcg ttgaaatcat cctccagtgt gttggcattg aagtacggat
    12901 ggctggcgag cgcgtaaatt tcgccggtct gggcgttcag gaggacgagc gcgcctttct
    12961 ttccttccag cagcctgtcc gccgcctgtt ggagcgaaag attcagattc accttgacgt
    13021 ccgagcccgc cgggggttgg ttgtagagca gttggttttt ccagacttct gaaaagggtt
    13081 tgccgtaacc gcgcaagaag gaatacacac cgcgttccag gccggattgc ccgtagagtc
    13141 cgttcgtata gcctacaacg ctgctgagcg gtgggtagag cagctcgcgg ctgtacgcgc
    13201 cgggctcacc ggtagtcacc gctaatgggt cgcccgcagt cgtgataatg ttcccacgcg
    13261 gagaatagcg gtcgtaaacc gcccagcgca aattttcagg tctggaaacc agcgtatccc
    13321 tgttccatac ggaaatcatg gcgttcttgc cttgcaggag cacaaacagc aggataagca
    13381 ctgcggagag aaattggtag gtagagtgcg agccttcggc gctcatccgt ttgcccggct
    13441 cagcgctcag cctgagtaaa agcaggacgc aagccatgca ggtcagcagg ttcgaacccc
    13501 cgtatgaaat aaaggggagg gtgatgccgg tgagcggcat caggcccaga ttgccgccga
    13561 tgatgaagaa tgactgcagc gcgaaaaacg cggaaatccc gaatgccaga tagcggccga
    13621 aggtgttttt ggtgcgtctg gaaatggcca cgccgcgcat gaccagtaat aatatcagca
    13681 gcaggagggc gctggttccg agcaaaccgg tttcctccgc aatcgcggaa aaaataaaat
    13741 ccgaaaccgc aaccgggata aactgcgggc tgccaagccc ggggccggtg ccaatcagac
    13801 cgccggaggc gatggcaatc tgggcttgca ccagctggta tgaggcgccg gccggctgca
    13861 gccaggggtt tagccaaata tccacgcgtg tgcgcacaat atcgaacagg aagtaacccg
    13921 ctgttccggc ggtgatgata agcagtggaa tgagccagag gaggcgtcgg cggtgggtgg
    13981 tgagcgccag catgaacaga aacaagaccg cgaaaagcgt tgcggtgcct aaatcgcgct
    14041 ggattacgag gagtacgccg accagcgcgg tgatcagcag cgtgggcagg atggaggcgg
    14101 ctatggagac gttcggccgg atttgatccg caaagaacgc agccagatag accagcaaca
    14161 gcagctttag cggttccgaa ggctgtaaat acactccgaa caggttcagc cacaacctgg
    14221 ggccagaacc gctgggattg accccgacaa acaaggtcag cgccaccagc agcagcccga
    14281 gcgccagcca gacgtacttg tattgcttca aaagcgcgac aagatcgcga attgtcatcc
    14341 cgccaatcat caccagaacg ccaaccagga accacagcag ctgttttccg ccaagcgcgg
    14401 gtgaaagccg ccagacggac agtaagcccc agcctgtcag cgcgccgacc agcggaatca
    14461 gggtcgggtc gtgcctgggg aggagtttgt cggccgcgaa ctgcagggcg gcaattcccg
    14521 cagcccagcc cagcagcggg agcaggtagc gcagttccgg ctgaaaatcg ctggcgtgcg
    14581 ttcgcaccgc cggagctaaa gccagcgcga acgcgtaaaa cgatacaaaa aggccaacca
    14641 gccacaaagg cgctggcagg gtcgcggtct ttttgggtgc tgcggtctcg ctcaaggtta
    14701 cttcacgtac ttggagatca gaagctgaag cttttcgcgg gtttcaggtc ggattttccc
    14761 ggggctggta ataagcgcgt ctttcagcgc gtcctggcag gcgcaggcgg gcgcgtcggt
    14821 ttcggattgt tccagaatgt ccacaagccg gatgagcgcg cgctgtgcca ggtctgtgtt
    14881 cctggccagg gtgcgcacga ccatatctac agaaacaggg tcttcgctgg tgtgccagac
    14941 gtcgtaatcc gtgacgtgcg ccatggtggc gtagcagatt tcagcttcgc gcgcaaggaa
    15001 gacctccgga gaagccgtca taccgaccag ggacatgccc caggcgcgga aggtattgga
    15061 ttcagctttg gtggagaagc gcggaccttc gatggtgata tatgcgccgc cttcgtgcac
    15121 agtgcctcct tcagccttca gcgctttggc aagcgcggcg ctgagatttg ggcagaaggg
    15181 ctccgccgtg ccaacatgca ccaccagacc ctccccgaag aagctgcgtt cgcgcttgtg
    15241 ggtgaaatcg aagacctggt ccgggatgac gacatccccg ggtgcgaatt tttcctgcag
    15301 ggaaccgcac gcgctgacgc tgataatttt cgtcacacca agctgcttca gggcgtagat
    15361 attcgcacgg tagttaacct ccgaaggcga aatcgtatgc ccgataccgt ggcgtgccag
    15421 aaatgccagc ggtttgccgt gcacttcgcc caaaacaatc ggagaggagg gcaaaccaaa
    15481 gggtgtatcg atgattaccg tctttttgtt ttccagacct tcaaaagcat acagaccgga
    15541 cccaccgatg atgccaaacg cgatcttgcc agcttccatg ttgacctctc tttgatttca
    15601 ggataagggc ttgatagaaa tgttgatgtc cacttcaccg caaaaaagaa catctccatc
    15661 ttttacaacg acgggctctt ccacgcgcag gtcttccagg taagaaccat tggtggattt
    15721 caggtcttca taccaccact gctgatgatg gtaactgaaa ctgccgtgca gggacgacac
    15781 catttcgtgc ggaagtataa aatcacactc gggagcccgg ccgacggtga ccttgggagt
    15841 tataaaaact tgcgcgttcg tggattccat taccgtggat gaaaggctga gcgggggaac
    15901 aagttcctgg acgctttggg ctgctttggt gacggtagat ttccaaatca cccacaagct
    15961 gagccccaga aacccataga gggcaatcag aaagaggatg cgtaagacca gcagtattcc
    16021 ggcgttcatg gcttgggctg cgattcaatc agcgagattt tggtttcatc cgtacccggc
    16081 agctcctggt tgtagaccag taaagtcttc ccgagccgga tgacatcccc gggtttcagt
    16141 accgcggtat gcacctggcg attgttgatg aaggtgccgc ctgtcgagct caggtcaaaa
    16201 aggaggtatt tcccctgttc cgcgcgcagc tgcacatggt cgcgcgagat catcgggtcg
    16261 ttgaggacaa tatcgctggt ggagcggcgg ccaatcttga taaccgcttt ttcaagcgcc
    16321 gcctgctctt ttccgttgat aatcaggtag gcatgcgtgg ggatcggttc ctccgcgagc
    16381 ggacgcggga cggtttgcag gcttgttttg gcttgctgcc tgtcgctatg ggcgtaatcg
    16441 acatggatag caatggcttc agcgctcaat tcggggtcag caatcacttt gatgcgtggg
    16501 ctgtgcagaa atgtgtaacc ctgctccgcc cagtaagctt cgagctctcg cgcggcatcg
    16561 tccaaagccg gccgcgcgct ttgccaggca tcccatttat ccgggctgat gagaatatcg
    16621 atgaggtccg gagcgcggtg ggtgttgggg ggttgggcgg caatcttttc ggaaatcaga
    16681 gcgaccagct ggctgctgag tgaactggca agccccggat agaggatccg gtccaaagtg
    16741 ccctcaaaaa gcgcgcgcag cttgttttca atggtctcca ggagcatatg ccttcccttc
    16801 tatcaggatt ataccaacga acgcgtacag gctgagcgca ctgaacgctt ctcagtgatt
    16861 cctcagtaac ctggggaggc cggtgcgctt gcaccaggcc cgctatttgc caggcagatt
    16921 cacagcgcgt aaatcggatt gctgaaaatc caggcgcggt gcatgcccag ataatccaga
    16981 tagacctccg cgcggtagct gcctggctcc ttggtcaggt aggtcagcac ttcgcggtcc
    17041 gtgtgctctt tcaccacggt gccgttgcgc aataaacgca cgtgcgtgcg cgcgggcagc
    17101 cgcacctgaa aagtaagtcc gtcttctgct gaaacctggt cgcccatcca gaatgtgcct
    17161 tcgttgctgt tcaccgtgaa gcggaaaccg cgcgccgaac gcggcaggtc gtacgcgatg
    17221 aaagaatgtc cggctcgcag cgcgtcgtaa atcaaggtct tatcctgggt caggttgcct
    17281 gtcagaggtt tggggagcag caggtggttt gtaatcgatc ggaattgctg ctcgtatgga
    17341 taaagcacaa cgcgcagcgg gcctttgcgg atttgcattt cgtgcgcgtc cgccccgccg
    17401 actgctacga taggtttgtt gtgtttcaga atgagttcat cccatagttt cagggtggat
    17461 tccagagggc ggaaagtcat cagttggggg aagaaggcgt gcagcaccgt ctttggcagg
    17521 ctggtggcgc gcgtcttgaa ttcgctcatc tggttccaca actcaatacc ggtaaagccg
    17581 ctcacctgcc agtcctccca attgaaggca gcttcgccga aggcaggcag cgcgtcttca
    17641 atcgggtgag cgataaacgt cagtccgccc gaagcgccgg cctgatggat tagctgctgc
    17701 gggtccgggg caaaacgggc aagttcgcgc gtgctgccaa aaatcagcag gtggtttttg
    17761 cctttcggga gggtttggtc gtgcacttcc tccccgacca gggtgagcag ccgttttttg
    17821 cctttttgca ggtagccttc catgccctgc acccacacgt tgtggtcgga aaccaggatt
    17881 gcatccaagc cggcttttag agcagcggcg gctaaatccg cgtgctttcc attcccatca
    17941 gaatacactg tgtgcatatg tagggcgaca atgatttctt ccattgctta cctcaagcgt
    18001 ccatctgaca tcgtgttctg attatatcct ctggcgcggg taggcggttg gatcctgtag
    18061 gtttgctgtc aaggctgaat agaaatgtcc tgatttgtgg atgatgtcgt cagggcggtt
    18121 tttttccctc gaaacgaatc gccccatttc ttcttttcga acatgtttca cgcatgccct
    18181 atccctgaca cttatccggc ggcatgcggc tcgatattgc ctcggccaag ccgctgggga
    18241 agaaaaaaag gtctgataga atcaaaaatc aagtcccagt ctcagcaatg gtcatccgaa
    18301 tcagatgtta aaattcgttc tttgggacgg agagtacgga tgaaaaggct gctactcccg
    18361 tgtatgtcag ggtgtgatcc ttcctttgtt aaaaaacctg gaggtatcga atgaggtttt
    18421 cacagctcct aatcaacatt cggagatttc tattttgcta ctttaggaca ttatcatgtt
    18481 gctcttacac aatcctgtgg gtttgtctac agtttaatct gccatctgat ataatagcga
    18541 tttgacgttt ttaccgcttt aggtataatc tttttgttat ttgccggagg ttgaattgaa
    18601 cagttttctg aatatcgcga ttatgatcac atccgttgcc ctgatagcaa gcattctctt
    18661 gcagagtaaa ggcatcggcc ttggcagtct ggccggcggc gatactggcg gcgtttacac
    18721 caaacggcgc ggtgttgaac gggtgctttt ctttgtgacg attggtctgg cggtcctctt
    18781 tgttgttttg gctttggtca gtgtgctcat ttccggataa cccctttcgc aacggtttga
    18841 cggcgtttca acaattccac acggaggtta aagacgactc gccagtcttg gcggttgtct
    18901 ttttttaaac tatgagaaaa tttcgctggc agctgctgat tatcctgttg actggcctgg
    18961 tggtaggttt gctgctggtt ttccagcagg ggacaaactt gcctgaagcg cagaacatcc
    19021 ccagcccggt caccggcggt atttacacag aagccttggt cgggaatttc ctgcgcctca
    19081 accccatcct ggatatggtc aatcaggcgg ataaggacgt agaccgcctt ctgttcaacg
    19141 gcctggtccg ctttgattcc agcggtatgc cccaaccgga cctggcggaa acctggagct
    19201 actccagcga cggcacgcgt tttaccttca gcttgcgcgc gaatgcctac tggcatgacg
    19261 gacggccggt gaccacgcag gacgtggttt acacgatggg gctgctgcgc gaagaaaatg
    19321 agttgattcc ccctgacctg cgagctttct ggtcagaaat tcaggttacc gcgctttccg
    19381 atacgattgt cgaattcgcg ctccccgaag cattttctcc ccttctggat tatctcagcg
    19441 tcccgctgct gccctcgcac ttgctgggca atctgaccct ggctgaattg gttgaccacc
    19501 cgttcaacac tgcgccaatc ggcaccggac cctacaaatt caaccgcttt ctggtaaaag
    19561 acggtctggt cagtggggtg gacctgctgg cgaatcagga ttactacctg ggcaggccgt
    19621 acattgacga ggtggtcttt cagtattacc ccagcatgcg cgcagcctgg actgcttttc
    19681 agggcggcga ggtggacggg cttggtggcg tcagcaatga tattctgccg gaagttctgg
    19741 cagaaccagg gctgaacctc ttctccacac gggaaccgcg ccttagcatg gtcttcctga
    19801 acctcaataa ttccgccaaa gatttcttgc aggaccctga gtttcgtcag gcgctgatgc
    19861 tttccataaa ccggcaggca atcctggata aggtcttgct cggtcagggg gttttggcgc
    19921 acggaccgat tctaccgggc agctgggctt attatgccga tcagggcagt tattcctttg
    19981 acccggacct ggcagcacaa aaaatcgcgg cactgggcat cacgcgcagc gaggctggca
    20041 cgctcacgac ggcggaaggc gtggaagtgc gaatcatctt gctcaccccg gacgatgaac
    20101 tgcacatgca aatcgcggat tctatcaaac agggctggga atcggtgggt gtggcggtgg
    20161 atgtgctttc catgccttat gctgacgtga tagccaggct tgacgcgcgt gattttgacg
    20221 ccgcgctggt ggaaattgat ctgtccggca ctccggaccc ggatccctat cctttctggg
    20281 cgcaatccca ggcccagagc gggcagaatt actcccagtg gaacaaccgt tcagccagcg
    20341 agtatctgga acaggcacgc gtcagcaatg attataatct gcggacaaag ctgtaccgca
    20401 acttccaaat cttgtttcat gatcagctgc cctccctgcc gcttttttac ccggtctaca
    20461 actacgccat ccgggacacc attctgaatg taaccattgg tccaatctac gaagccagcg
    20521 accggctgaa tgccatcaat acctggttca ttctcgcggg gaatgcccct gaaggcgaat
    20581 gagcatggcc gcccgcagag cactcgcgcg taaaccgggc tgcaccagcc tgatacggga
    20641 agaaaacgtc tctaaggcgg acccgcgctt gcgcgcgatc ggcgcgattg acgaagcaag
    20701 cgccagcctg agcctggcga aagctttttt gcctggcgag cctgagaagg ccgcacttga
    20761 gacctgtcag gagcatcttt cgctcatcat ggcgggtctg gcgggctgga agcaggctga
    20821 cttcccggaa agtgcggcgc gcgcgctgga ttggctggaa gctttattga ctgaaatgga
    20881 aaacagtgtg gaacttcccg gcgggtttct gcaccctggt aagtcgccgg ctgaaggcgc
    20941 gctggatctg gcgcgggctg tcgtgcgtcg ggcggagcgc gaaacggtcg cattggcgca
    21001 gtccgaatca ggggcggagc tgcccgcgga cgcgcttact tatctgaacc gactgtcgtc
    21061 tttatgcttc ttgctgctag ccgcgcaaat cgccgggcaa aaacccgaat aagcaggacg
    21121 gcggctgcat gataggtacc ttcattaacg tcgcggcaat cctgttgggt gggctgctcg
    21181 ggctgactgc cgggaacaaa atccctgcca aatttcgtca gacgattgtt tcaggcctgg
    21241 gtttgtttac cctcgcgtat ggggtgtatc tctttacgga aacccgcaac atgctcatcc
    21301 ccttgggtgc gctaattctg ggcacgattg tgggcgagtg gctacgactt gaagaacgcc
    21361 tggaaaattt gggcgctttt ttgcaaacca aatttaacca cgcggaaggc tccggcagcg
    21421 cggatgcaca gcgttttatc aacggctttg ttgccgcctc cctgctgttt tgcattgggc
    21481 cgatggcgat attgggctcc attcaggatg gtttgagcgg ggatttccag atgctggcga
    21541 taaaatcgct cctggacggg ctgacgtcgg tggcgttcgc gtcgtcgctg ggggtgggcg
    21601 tactcttttc ggcgttaccg gtgctggtgt atcaaggcgg catcagcctg ctggcgcgcg
    21661 cgttggggca gggttttgat gcttcggtcg tggctgagat gaccgcggtc ggcggggtga
    21721 ttttgacagg catcgcgatc agcagtctgc tggaaatcaa gaagatccgc acgggcagtt
    21781 tcctccccgc gctggtcttt gctgttttga ttgtgctggg cttgaacgcg atgggcgttt
    21841 cctactaaag tccaacttca gctgcgaact gattcaaaca aaacgcctgg cgggcaagca
    21901 gccgggcgtt ttctgttgca ggtctatgct atctcagcag cgcggcgtct acggtttcat
    21961 ccagggtgtc catgtagacg atggtcccat tgacagagaa ttgcggggtc gaatcaatcc
    22021 cgagcgaatt ggcatagctc atgatctcat caagtttggc caggttcgcg tcggactgca
    22081 ggcaggcgct gaacttatcc gtgtccaaat ccagttgtcc ggcgtaatcg atgagattgg
    22141 cttccgcgaa tgcgcccgta taggttgagt aagtgaacag gagtttcttg tactgccaca
    22201 ttgcgttttg ctcggcggcg caataggttg cttcggctgc gtccgcggaa tcctctgcga
    22261 ggaaagcgta gttgtggaag gtgaggtata ccttgccagt atccacatat ttttcgatga
    22321 aatccgcttc atagttcagc gcgaagtttt ggcagtgact acagcggaag tctgagaact
    22381 cctccacttt caccggcgcg ttggggtcgc cgatggagaa gccgtcctgc atatacaact
    22441 ggtctttatc aagtgtccac ttggggagca tcaccagcag gaagactgcc agcgctgccg
    22501 cgccaagcac aatcagaagc gtgaccgttt tctttttgga ggcttgcttg cgctgttcgc
    22561 gcaatttttc tctttgggat tgtgttccgg gcataaacag actcctctgg taagaataaa
    22621 acagattttg ccacagccgc gccgaattgt aaagcgaact gcatcaggaa gccgaaacag
    22681 cgcggataag cacgggtatc agccgtcctt cctgccggaa gcccagccgc ttgtaaagct
    22741 ttagggaggc ggcgttgttc gtctgggtgt tcacggtgaa atccgtgctg ccgcgcgatt
    22801 ccgcttcgcg gatggtatcc agcgccaggc ctgaaccaac gccctgctgc tgtgcctgcg
    22861 gcagcacagc cagcctggcc aggtgggcgc tgtcaaagct cgccgcggtc atctggtacc
    22921 cgaccaattt gtcctctaaa aaagccgcgg tgctgtagga acaagcgcga aaggcagcct
    22981 gcagcgtctt cgtgttcagc tgccagggcg ggctgaaagc ggcatggtcc aattcctcaa
    23041 tttcggggag gtcgtccgct gttatcggac ggatgctgag cctggggtgc ggcttgactg
    23101 ggggtgtaag cacgccagtc cgaatcaagg tgataatctg cgtgtacccg gcaaagccat
    23161 ggtcgctaaa aagctgctcg tgccagcttt tgaaggagag ggtgaagagc gccggcacgc
    23221 gggccgctcg caagtccgcg agcgcgcgct ccaggaggtt cgtaaagtat ccggaatgct
    23281 ctccgtcaga ttgggagagg aaaaagcgca gccaggcggc actgtggttc tccggttcgc
    23341 aggaaatgag tgccttgacc tgggattcgt ctatcagagt gtaacaaaac tgtccctcca
    23401 ggcgctcgcg gtctgaaatc caatccaggt tggagaacga tagcggcgcg gagttcagga
    23461 aagagaccag cgcgcttttc ggataagcac tggctggtaa tattagcggg accatagttc
    23521 gtaccttctg ctcatggctt ctgtgcgctg agggagaatc cgtgccgggg ttccagctca
    23581 gcgttggaaa actaccctgc cgtccaccag ggtcatctcc ggagcaagct gccacaaatc
    23641 ctgcgggttc atgcggaagg gatcacgggg gaggacaatc aggtcggcgc ggtaccctga
    23701 ggcgatacgc ccaaagcttt gccccagacc gcaggaacgg gcggggtttc ctgtgtaagc
    23761 atccaacgcc tgggccaggc tgatgcgctg ttccgggtac caaccttgcg cgcccggctc
    23821 tccgctgacg cggcggcggg taacggcggc atgtaagccc aggaagggat tggcggattc
    23881 aaccggcgcg tcggaaccaa aaataagctg cgtcccctgc ttgaggagcg cggaaaaggc
    23941 ataggcgtta accgagcggc ttccccagtg ctggtcggca gtgaacatgt ccgagggggc
    24001 atggataggc tgcatagaag caattacgtc cagcgctttc agacgcggct ggtcctcggg
    24061 tgtgatgagc tgcacatgtt cgatgcggtg cggcaaaggc gggcggccgt tttcccgctc
    24121 ccaggcgcgc agacgctggt acccgtccaa cacagcgcgc accgccaggt ccccgatggc
    24181 gtggatagcc agaggaaagc ccacagcggc ggcgtttaag ccctgcgcgt aaacttcgtc
    24241 ggcgtccatc agcaggatac ccgcgctgtc cgagttttcg tagggggaga gcatcgccgc
    24301 ggtctggggg cccaaagcgc cgtcagcgaa gtatttgagg gagcccagga aaaggtgttc
    24361 cgtgccgctg ccgctgcgca gcccctccgc ggcgatatcc gccaggcgct cggcgtgcaa
    24421 gtttttgcgc acgcgcaaga gaagctcacc ggcggcgtcc agggctaaca gggcttgaaa
    24481 gcaatcccag cggtcaaagt cgtgcacgct ggtgagaccg taggaaagca gcagtgcctg
    24541 ggcgtgctta agcatgctct ccagcctttt gcctgccagc ggggggacga ctgcgtccac
    24601 cagctgcacg gcgttttcca gcaggatgcc ggtaggtttg ccttgcgaat cccgcaaaat
    24661 acgcccgccg gcggggtcag cgctgcttat gtcgatgcca gcggcgcgca atgccgtttc
    24721 atttgcccag gaagcgtgca gagatttggc gtagagcacg gcaggatgcc cggcgctgac
    24781 ctgttcaagt tcggcggcag tgccgtaatt ggccggttcc cacgcgttct ggttccaccc
    24841 gtagccgatg acccactcac cctggctcag gccgcggctt ttcgcttcca cgcgcgccag
    24901 gcattccgcc agcgagccag tttcgcagtc cacggctgcc aattgctccg ccagaaatgc
    24961 caggtgcagg tgggaatcca ccagaccggg ccagacagtc tgcccctgca ggtcgatttg
    25021 ccggcatgaa tggcgcaagc ctgagctgaa ttcctcaggg aaggcaaggg cgctgatgac
    25081 gccgtcctct accagcgcgg actgcaggag tgtttggctg ccgctgccct gatagaaata
    25141 accgttcttg agcagcacgc gctcagtcat ttaaccctaa cgcgtccgtg attccattga
    25201 gttcttcgtc ggaatagtag tagatggtaa tgcttccgcc ctttttcccg gactgtaagt
    25261 tcacttttgt gttgaagaac tggcgaagtt tattttccag cgcctgcagt tcggccgatt
    25321 tgcccggttc tatctttttt ctgggcgcct tcccgagcag ctttgtaatc aggagctccg
    25381 tctgacgcac attgagccca aggttcagga ttgtttcgag cgcggcttca atggcgcgcg
    25441 tggaattgag cgccaacagc ggtcgggcgt gcccttccgt gattaagtcg tcctgcagtg
    25501 cttgctgaac gctggctggg aggttgagca gacgcagggt attggtcacg gcggcgcggc
    25561 tttttccgac cttttgggca atgtcctcgt gggtgagggc gaattcgttc agcaggcttt
    25621 ggtaggcgtg ggcgcgttcc agcacgttga gatcctcacg ctgcacgttc tccacaatcg
    25681 cgaactccaa ctgctcttgc agggtggcgc tgcgcacgat tgcgggcacg tcctgcaggc
    25741 cggccaggga agctgcccgc cagcggcgtt cgccagcgat gagtgtgtac cgctcctcgc
    25801 cttcattctg caccaggatc agcggctgca aaataccgtg ttcctggatg gaattcgcca
    25861 gttcctgcag gctttctgct gagaaggctt tgcgcggctg tctggggttc ggggtaatct
    25921 tgtcgattgc gatctggacc acctgattgg ctggcaggga ggagtagctc tcttgctgcc
    25981 atgtgggaat caacgcatca agtcctttgc caaggccggt ttttcgtgcc atgctgttac
    26041 tcctgtacct cggggatatg cacgccgttg gagctgagca gctccatcgc cagagcccgg
    26101 tacgcttttg ctccgttcga ttcgggcgcg taaactgaaa tgggcatgcc aaaagagggc
    26161 gcttccgcga gtcggatgct gcgcgggatg aaggtgttga acacccggtt cgggaacacg
    26221 cgctgaactt cgctcatcac gtccgcggag aggttggtgc gcccgtcgaa catggtcagc
    26281 accacgcctt taatgtccag cgtcgggaaa agcccctgcc gtacgcgccc gagcgtctgt
    26341 gtgagctgac cgaggccttc cagagccaga tactcgcact gcaccggaat gattaccccg
    26401 tcgaaggcgg cgatcagccc gttcaaagtc agcagaccga gggagggcgg gcagtcgatc
    26461 aggatgtaat catagcggga gcgcagcggg tcaattaact tgcgcaggcg ggtttcgcgc
    26521 tccggcaggt caatcagttc aatttcagcg ccagccaggt taggggagga gggcagcagt
    26581 gaaaggtgaa agcgcgcgtt gtgcaggata tggtccgcag caggctggtc accaatcagc
    26641 gcttcataga tgccgttttg gacgctgttt ttatcaacgc ccaggcagga agtggcattc
    26701 gcctgcgggt caatatccac caagaggacg cgctgttgga atgcacccaa atacgcgccg
    26761 aggttgatac tggtggtggt tttcccgaca ccgccttttt gattcacgat ggtataaact
    26821 ttgcccatac tttcctgtca gatgatttgt gtaagcgcgg cggaaatctc cacgctggtg
    26881 ggagtgctgt ggaaatgcct gcgggtgacg gaggcagccg ccaggcgcac cgcgaaacgg
    26941 gctgcctccc aggggtcctg ggtggaatac aggcggtgga aaaagcacgc ggcaaaaata
    27001 tcaccggcgc cggtatcctc tgccaacgcg acttttggag ccggaaagcg gcgcacatca
    27061 ccgttccagt aaacccgagc gccttggtcg gattctgtga ctgccaagag gtggcacatg
    27121 ctggcccact tttccacttt tttctcgtcg ttgtgcaggt cttccaggct cagaaccacc
    27181 gcgtgggcag cctgtaaaag cttcgggctg tacgtccagt ccactgggaa aacccgcccg
    27241 tcctgcgcaa aactccgcag ccaaccctgg ggggtgaggc acagcaaact gcgcggaaag
    27301 gcagtataga tatcggggtc aatctctgcg gcaaccgggc ccaggtgtac gattggtgag
    27361 ttcttccatt cttccggcac cgacgaagcg tccaggcggc gggcggagtg gtacatatac
    27421 tgcagccgac cggaatcggt gggaatgttg cggaacaccg tgctctgggc ggcaggcagc
    27481 gtgcgcacct gtatgccagc gtaggcatcc aggtcggtat ccgcggcgca gctggtgagg
    27541 atgcctaccc gccggccgag cgctttggct gtcagaccag aatagctgac ggttccgccg
    27601 gggcggtacg tgccgtcgtc cagcaagtcc tgggtgatgt gcccgatgac aaggtaatcg
    27661 ataggttctg acgtggataa ttccatgcgc ctgctttgtc cgctcataaa ttgttcaaaa
    27721 aattatagca tggttcaccc ctgggtaccg gggcttgacc gttttctgcc cggccgcctt
    27781 gagcgccagc tgtgggggag ccagcccgcc aaactgaaaa tgattgtgat aagattttag
    27841 atgagcctcc tgttggagta tgactgcatg gaaaaagata tcactaccta ccaaaaagcc
    27901 ctggaagact ttaaaaaagc gcgttccaaa gctgcgctgc agcgcttttg ggcaggcatc
    27961 cgcggaaagt cgctggacct gctcccttat gatgagatat ccgcaaaatt gcaggcgttc
    28021 aataaggttg accgcgggct gcagacggta aaactgaaag acattgtcgg cagcgtcggc
    28081 agaaatgagg attttgaccg caatttcctt cctttaaatg acgctgatat cattcgctgg
    28141 gcgcaggtga agaccgcaat gaccagccct accggcgccg gcctccctcc ggtgagccta
    28201 tataaagttg gggacgcgta ttttgtgctg gacggcaacc accgcgtttc gattgccagg
    28261 gaaatgggat tggacgcgat tgaggcttat gttaccgaaa tttacacgcg cgtgcctgtt
    28321 tccacggaca tcaccgcgga ggatctgcac atcaaagcga cgtatgtcgg gttcctggaa
    28381 aaaacccggc tggaccaaat cctgccgggc gtcgatttca gcctgaatca aacagagaac
    28441 tatcccctgc ttgaagagca tattgccgtc caccgctatt acatgggcat cgaacaaggg
    28501 cgggaaatcc catacgaagc agccgtactg gattggtacg aacatgtata ccaaccagtg
    28561 gtggaaatga tccattcttc gggcctgcgc gaggaattca agcatctgac gatcaccgat
    28621 ctctacctgt gggtgctcga ccagcagtcc attctgcagg aagcgctcgg cataccaatc
    28681 cgctcagcgc atgccgcgga gtacatggcg ctgcaggaag gcaagacgat gagacctcca
    28741 acctcaccga ctgaacagca ctttgaggaa acggtgtttt ctgattccgc caaagcggca
    28801 ttttccctgc cgtatgtgca ggataatccg gaaggatgcc ttttccgcga cattctggtg
    28861 gcaatcggcg accatgacga ttattggaag gcgctcgagc aggctgtctt gataaacctc
    28921 tgccaggtcg gacatgtgcg cggtctgcac gtcatcagcc ccagcgacac gcttgatgat
    28981 actgcccacc aaatgatgga gctgcgtttt gaaaagcgtt tggctgagat ggggatgagc
    29041 ggaaagctcc tgatcgtttc cggggagatt accaacaagc tgagcgaaca cagcttgctt
    29101 tcggatttgc tggtgctgcg tctggctttc ccaccgaccg gcggaatcct ggacagactg
    29161 acatcgggca tcgggtccat cctgcacagc gccaaacggc ctgtcctgat tgtcaaggag
    29221 caggcgctgc cgttcgaacg aatgatggtc gtatacaacg gttcgcccaa aagcaaagaa
    29281 gcgctgttcg tgggggcgta ctttgccggg cgttttgata cgaaattgtg tttattcaca
    29341 cttgatgacg gtacgcctga ccttgagtcg gaaacgagct acgcgaaaac ttatctgcga
    29401 aaattgaacc tggattttga gttcattttg cggaaaggcg ggaacttagc cgaagcagcg
    29461 ctggaagagg cggccgcgtt cggagcgacc acaatcgtcc tggggggata cagcgggaat
    29521 tccctgttcg accgattgtt cgggtccacc attgacaatt tgctgcaaaa aaccgagctg
    29581 ccggtattaa tctgccaata gcggcaggcg cctgatcacc agttcggtaa tatcgtcgct
    29641 gaggggcacc tcccggcgga aactcgcaag gtcttccgcg accgcgtcca gcaatgcacc
    29701 tggcgaaaga gccgcgcagc gctggagtac tttctggtag cgggtcattc cgtaaaaact
    29761 gccaagctta ttgaacgctt cagtaacacc atccgtgaac agcaccagac cgcagccagg
    29821 ttcaatcgca agaacgcgct caacgtaaac agcatcctcg tagatgccca gcgcggtccc
    29881 gcctctttgc agcgggctgg cagttttggt tttcgggttc agcaggaagg gcggcagatg
    29941 gcctgcgttg gcaaaggtca ggcggtggtt tagcgggtcg agtaccccgt agaagcaggt
    30001 cacaaaaaaa ccgcgctggg aatccagctg caatagttgg tttacccgct ccagtgtttt
    30061 ggcaggcgag tcggattcca gggcgaccgc acggatgagg gtgcgcgtga cggtcatgta
    30121 cagggccgcg gcaatgccct tatccgagac gtccgcgatg accatcccat acgagccgtt
    30181 ccggagcgca aagatatcat aaaaatcgcc gccaacctgc ctggcagtct gccagcgtac
    30241 gtccagctcg tagccattga tttcgggcag cttctccgga aggaaagtct cctggatttg
    30301 gcgcgccaga tggaattcct gctccacgcg ctgctggtcg gcctttacct tctccaggcg
    30361 ttcattctgg atggcgaagg agatttgttg ggccactccg ttcaggagct ccatgcggcg
    30421 ttcccgtttg ggtatgtccg ggtcgcggca gatcagaacg ccgtaatcct ccccgcgcat
    30481 ggcaagcttg taaatcagat agaccgagtt ggaggctttt atcagcggtg agtccgtgtc
    30541 ggactcgctg cgcagtgcgt cattcgccag caggttgtat gttccccgga cctcatcgga
    30601 cctgacaagg ttgggcttgc catcctcaga accggggatg aacgggaatt ccgccctggg
    30661 gatgcgcgcg ccgtaaagac gctccagctg ccacgggtgc agttgttctg aggcggcgtg
    30721 tttcagttcg taggaatctt cctctggcag ccacctgaac gtggcgcacc cttccacgcc
    30781 aatcagcata tgcaggacgt aataaatccg gtccatggct tccaccaggt cgggtgtggt
    30841 cacgatggtt tgtgagactt gcagcaatac tgaggagata taggtttctt cctggcggga
    30901 gttgagcaaa ttgatgttct gcgcgctgac tgccacctgc tgcgcaatcc cctgcagaat
    30961 ggcgtatttc tggcgcccca gcacactttc cggcggggcg ggttggtatg cgtctgtgct
    31021 ggcatgcagc aggaaaccca gcagttcctc gtgcgcgatg agcgggagca gcaaaattgt
    31081 gtcttcgggc tccaggccca gcatcgcgcg cagatcgggc tcacactcct cggccgggaa
    31141 tgccatgggt tccaatttct cggcagcctg gacgaatacc tttggcctgc cgacccgtga
    31201 aggaagcgtg gtattgagtg tcttcaggtc ctcgccgaac acgccttgca ggtaatagct
    31261 gcgctcctcc ggttccaaca ggaagaccgc gccctgctta ccgccaatca ggagcgtgat
    31321 gagctggcca atcaaatcag tcagttcatt gatacgcgtc agggattggg tggctttggc
    31381 aacctgcagc aggatagtag aaatccagtt ttggtctctg gattcctgcg cgaggcgttc
    31441 gttctccacc gcgataccgg catagcgggc gaaggaagcg ctgatgctca cggattccgg
    31501 accgtagcgg ttggcggtgg catggtgcaa cgtgaggacg cccagctgct tgccgccggc
    31561 gctgagcggc gcgctgagcg cagaaaagcc gtcagggagt ctgagggctt ccgccagagg
    31621 gtccggtttg cccgaatttt gccgcacgac cggctcattc cggctgagcg cttcagggta
    31681 ccaggccagc tcgccgctca ggcagttttc caggtgaggg tgctcggcag tccgctggga
    31741 tttgtaggct gccaggttca agcagcccgg cgcttccccg cttttgcggg gcgtttcatc
    31801 cagcagccag atcgcggccg cgtcgcaggg aagtacggcg ctaatgctct ccaggatgaa
    31861 gtcgaatttt tcctcgagcg cggagctttc cgtcagcttg cgcgccacgc tctgcaggct
    31921 ttcagccaca cggctgcgcc atttttcggc gttgtacagc ttggcgttgc gtatagctat
    31981 agagatgctg gctgaaagcg tggagagcaa gtcgatatcc gctgcgtcga aagcgttcgt
    32041 tttggcgctc tgcacatcca gcacgccaaa cacctcccgg ttgaacgcca gcgggatgca
    32101 aagctcggaa ccgctgtgtg cgccgatgat cgggctgggc atgtattccg ggtcgcggcg
    32161 cacgttactt gccagatgaa tgcgatttct gcggaccgtc agggggatga gaccttttgg
    32221 agcgtcaagc tcgaaggaga ccttatgctc acggtacttt ttcgcgcgcg aaccgctgcc
    32281 agagtgaaaa tcaatcgtgt tccgcacgcg gtccagcagg aaaatatgta caaacgggta
    32341 tttgagctgg ctgtgaatca tctgcgcgac tttattgagc agtgtgtcca ggtccaggat
    32401 gctggagatg gcttccgtca gaccggcaat cacgtcgcgt tgttcggtgc gttcttcgag
    32461 ttccgaatag agcctgactt tgtgcaccgc caggccgatg ctgtccgcca gcgcggaaag
    32521 gacgaccatg tcctcttccg tgaacacatc cggctggtcg gattgaacat ccagcacgcc
    32581 caatacgctc ccgccagcct tgagcggaag cgccagctca gctttcgtct cgggcaggga
    32641 ttcgaggcgc ccgtagcgcg gttccttgct cacatcgttt gccagcagag cccggccgct
    32701 ctgagcgacg tgaccgacga tgtggtcgcc caggctgatt tgcgtacctg ctggaagttc
    32761 aaacgcaggc tgatcggaat tttcactgcg cgcgctggcg cggaagagaa cggtgttagt
    32821 tttcctctcg agggtaaaaa tcgccacgta atagaatccg aaggcgcgca ggattgcctg
    32881 gctcacttcg cgggccagtt cgtccaggcg ggtgatatgc gcgatgctgg ataccaccga
    32941 ggcaatcagc gcgaggcgtg ttttctggtt ttcctggcgg atttccgagc tcaacagggc
    33001 caattccatg ctgacagcgg cggcaagcgc atccacgaag caaatttcca cggcgtcaaa
    33061 gggctgtaat tcccgcttga ctaccagcga acccagcacg gtattccggg taagaagtgg
    33121 aacgccgagc agcgcggatt ggtctgccac attcgtatcc atgacgcgct tgccggagcg
    33181 ttccacctgc aggtggattt ttgttaagct ggcagaaagg ccctggcatt cattatcggg
    33241 aaaggggctt gggtcaggga aatgaaagct ggattgcgcg gagagttctt gttggatgaa
    33301 tttcgaaacg gagcaaaggt aagcttccgg agggtcgtgc ttgccggact gctgacaaaa
    33361 atccgtcagc gccttccaca aatcggcctg gtcttctcct gtaaaggttt tcacggctgg
    33421 gttttctttc gcttggtcat tacaagcgtg ttcacaccgg gctgcgaaaa atcgaaaaac
    33481 acctcatcca tcaatttacg catcgtgtac acgcccagac cgcgctcgcg gcgcatttcc
    33541 agcgggctgc tgatatccgg gtccggaatt tgatcaggat cgaacggcgc gccgcagtct
    33601 ttcagggtaa tcgtgaggcc ttggctgctc gcctcagcag taacttcaat cgtccccagg
    33661 tcttcgccgc agtaggaatg gtcgatgatg ttcgccgcgg cttcattgac tgaaagctcg
    33721 accgcgtata actctttggg agagaagccg attggggaag cttccgtgag tataaactcg
    33781 ttgatggcac caagcgcctg gtaatttgct gggaaaacct gagtggacat gcggcaaccg
    33841 ttggtgaaat attctggttg gattataaca tgcgcctcag gcgcttcaat cgcgtgtaaa
    33901 ataaggctat gactaaagag agcgggcgtc cgacaatcaa ggaaatgcgg gaggagaatc
    33961 gcccgcggga acgcctgatt gcggagggcc cccaggctct cagcgacggg gagctgctgg
    34021 ctgtgctgct gggcagcggc aaagcggggc ttaacgcgat tgagctcggc aaccaaatct
    34081 taatggaatt gggcggtttt tccggcattc accgcacgga catatccgaa ctggaaaata
    34141 tctccggggt cggactagca aaagccgcgc gcattaaggc ggctgtggag ctgggcaacc
    34201 gcctggccaa agagcgcacg gaggccaaaa ctgtgattcg cagcccagag gatgtggtcc
    34261 gcctggtagg cttggagatg cgcggaaaag agcaggaaga gctgtgggtc atctggctgg
    34321 acgtgcgcaa tcaggtattg gggattgacc gcttgtacaa gggctcgcag gattcatctt
    34381 ccgtgcgcgt cggcgaaatc ttcacacgcg caatccgcaa aggcgcgaac gcggtcatcc
    34441 tggtgcataa ccacccttcc ggggatgcct cagaaagccc tgaagacgtc aatttgaccc
    34501 gcgcggtcat cgaagccgga cggctgctgg acatgcgcgt gcacgaccac atcattattg
    34561 gcagggatga cttcaactcc attaagaaga accaccccgc tttgtgggtt tagagactgc
    34621 ttagtttttt ctcgaaatcc tgcggataag gcgcccgaaa actaagctcc aaacctgagc
    34681 tcgggtgttt gaagccgatt tcataggcat gcagcatcat gcgctctgct tgcaagcgcg
    34741 catcctccgt attgagcgct ttgggatgat agagcgcgtc tcccaggatt cccaggccgg
    34801 ctgcgtacaa atgcgcgcgg atttgatgcg tgtaccctga aaaaagcgca cattccagca
    34861 gcgcaaaact ttccccgcgc tgcaggacgt ttacgagcgt acgggcgggt ttgccgcgct
    34921 tcaggtccac gcgcgtgcgg tgggcgcggt ccgcgttcac ccgtaagggc gggtccaggt
    34981 cttgcgactg ccactgcggg tttggagcag ccaggcagtg ataaagtttc ctcagcgcgc
    35041 gttcccggaa actctggtta agctggcggt gggcttcagc gctgcgcgcc agcagcatca
    35101 ccccgctggt ttccttatcc aggcggtgta caatccaaag cttccccact gctggcgaga
    35161 gcgcctgcgc gagatgcggc agcccggggt ggtagccgtc cgggatggag agcaatcctg
    35221 caggtttgtt taccaccagc aggtccgcgt ccgcgtataa aatccaggca ttcgggtcac
    35281 taatgtccgg gggggtgttc atcaagtaca atcataccat cgcgagcccg ggaaatttga
    35341 tgcgcagcca accgggataa ggagcagcca tgttcacagt tattgttact atccaggttg
    35401 taccggaatg cgttcaggac ttcatttcta tcacgcgtta caatgcatca aacagccgtc
    35461 tggaggctgg tgtggtgcgt ttcgatttct ttcaggagct tgaccggccg gagagctttc
    35521 ggttggtgga aatttatcgc gagcaggcgg accaggctgc ccatcagcag accgagcact
    35581 acctcaaatg gcgcgaagcc gccgcgccga tgatggccgc agagcgcacg cgggttttcc
    35641 tgcggaacct ggatccgacg gacgcggaat ggctgtaagc tctttccctg gctgattcag
    35701 cttctggcat ccgaggaaat tacaagcctg gccgcggtaa cgataaataa gcagcggttt
    35761 tgcgaccgct gcctacgccc tcttgagacc aggtagaaaa gttccggcta ttccggcgcg
    35821 ctgaaacaac tggtagtaac gacgcctggg ccgacatgca ccacaatcgc cgggggcaca
    35881 acgaagattg gaatgtcagt caaatgcagc tctttttcca actgggcttt caactgaatg
    35941 gcctgttctt ccgcggcgca ctggctgatg gtcagatgtc cgttggggtt gttcctgagg
    36001 attgcccggt cgagatcgac aatcgtcgcc agggcttgct tcagggtgcg caccttgtca
    36061 aagggttcta cctgcccgtc cttgatggtg agaataggct tgatttgcag cacactgccg
    36121 accaaacgag acgcgccgcc aattcgtccg cctttgtgca aatactccag ggtatcaacc
    36181 acgaaataga cattttcccg ggctgccatc gcgctgagct tgcttttgag ggtttcaatt
    36241 tccatgcctt gttttgccca gcagctggct tgcaagacga gcgaacccaa gccgccggca
    36301 atcgtttttg tattcagaat atggatgcgg tcgctgtgga attcttccgc cgccactaag
    36361 gcgctgcggt acgtgccgct cattttgtca gaaggggtga tgaccagagc ggtatccccg
    36421 gcatccagga ttttctggaa ggtgggcgcg tacatggctg gcgggggagc ggcggttccg
    36481 gggagctgtt tggaggccgc cagtttcgta aggaaggtct cggtatcaat ttcggtgtca
    36541 tcccgatagg atacatcccc aaaggtgatg atttgcggca gaacgtggat cccttgcgcg
    36601 gataaccggg tgagcgggat gccgcaggtt gtatcaacaa caatctgtat catactaaaa
    36661 gtcctttctg tcaggcggcc gtacgataag ttatcggcct ggataaaaaa gaaattaatg
    36721 gtaaaaacag cagatgctga acttggcaag cgtggcagct gctaaggggc ctcaggacct
    36781 gggctgcccg cagttcgggc agattttttc cttcttttcg aggataaagc cgcatttggc
    36841 gcaggtggaa gcctggtttt cccccagcgg cgcgccgcaa gccaggcagc gcgcggaggc
    36901 aatcgggttg gcggtcaggc agtaggggca gacggtgcgc gccagacgct cgctgagctc
    36961 ctggcctgtg ccgtgctgcc gcgcgaagct gtccaccacc tgccagacgt tctcctgcag
    37021 ctgcagggat tctacgtcct gagcgatatc gtccatccgt ccgattagcg tccacgggtt
    37081 gcgcagcgcg ttgagggcgg tcatcccgag gctggcagcc agcccaaacc aggcttgatt
    37141 ggaaagctgc acgctcacgc cgtccgccac cttttggagg cttacggtca gggctgtggc
    37201 gccgccgctg gagaggttcc ggcgggtggc aatctgcaca gccatattgt ccgggccgcc
    37261 gaggcgctgg acctggtaat taccgcggtg gaaatatgag atcaaatcgc gggcaatatc
    37321 ctcaggggaa aaacttccat ggtagatgcg tggtgtcatc gctgcctttt cttgtttgca
    37381 gatagattat aatcgggata tctggaagca aggatagatt ttatgaattt tctgaaaaaa
    37441 caccgtttgc cttggttgtt ctccgtgttg attttgctcc ttctgacggc gctcagcctg
    37501 ctgccggcgc tggaaaaccc ggtgttggcg cagtttccta cagtttcgat tccgacggtg
    37561 accagctctc cgcgcggcgc gtatatccgc gttctgttga cgaccaccga gtacgaccct
    37621 atcaacgtgc gttccggccc cggctccgtc tacgagctgg tcgggatttt gctggtaggc
    37681 caggaagcca aagcttacgg ttactacgac cagtatattc aaatcgagta cccgggcggt
    37741 ccaggcggaa gaggctgggt ctttgccaac aacgtggatg tgattggcgg tccaattccg
    37801 cttctggaac cgccggcaac cgcgacgccg gctgtcacca aaacaattga ccctaccctt
    37861 gcagcgcagt ttatctacac ctcgcagcct tccaggctgc caaccttcac cgaaccgccg
    37921 gctctgaacc tgcccaccta tcagaatatc accggcaaca cagccccggg cgggctgccc
    37981 ttggggctgg tgattgtggt gttggccggg ctgggggtct tcctgacgct gatagccatc
    38041 ctgcgccggg gctagacctc gtcgggtttt gccgcctccc tctcagagcc tttgatcttc
    38101 attccacgca cggacattcc acgcacggac aggccaggtg cctgcccttt atgttaaaat
    38161 gaatgcctat ggacgttaag acagtttcta ccaaccgcaa agcgcgtttt gaatatttcc
    38221 tgcaggagac ctttgaggcc gggcttgagt tgaaaggcac ggaaatcaaa tccattcgca
    38281 aggggcaaat cagcttgcag gaagcgtacg tgcgcaccga cggcaaacaa gcctggttgg
    38341 tcggcgcgca tgtcgcaccg tacgagcatg ccagcgctta ccagcacgat ccggatcgtg
    38401 agcgcagact cttgctgcac aagcgcgaaa tccgggagct atgggacgcg gtgcgcatta
    38461 agggcatgac tatcgtgcct gtgcgtgtgt acctgaaggc cggccgcgcc aagctcgagc
    38521 ttgccatcgc gaagggcaaa aaacagtacg acaagcgcga agcgattaaa cagaaggata
    38581 tcgaacggga agagtcgcgt gacgaacgac gttactaaac cggcacgcgt ataagcctgc
    38641 gcggaccaga agcttctgtc cgttataata gccgcatgaa acctgataac aagatcaccc
    38701 gcagagaatt cctcaagctt tccggcatgt tcatcggagc gatgatgctt ccgcgcccca
    38761 agggcgtgtt tgccgcagcg gcgcgccaat tcccgcaggc ggataagctc gggcgtattt
    38821 gtgtcgggat ggaaggcgcc tggttcaaac tcaagaccga acccaatgtt aacgcgccgg
    38881 aagccggcac ggtctggcgg gatgacgtgc ttgtttggag ccgtgaggtc gttgccaacc
    38941 agctggattt gaacgtgtac aaccagcgtt gggtggagac gcctggaggg tacctgcagt
    39001 cctccatggt ccaaccggtt aaaaaccttc ccaaccagcc cttgcagagc ttgcccgtca
    39061 atccggacgg cagccaaggg atgtgggtgg aaatcactgt acctttagtg gatatcaaac
    39121 caacccgggt gcccgctgcc tccttctgga tccgggaagt gaacaagccg cgcatctact
    39181 acagccaggt tttctgggct tacgacgtgc gtcaggagaa cggaatcacc gaatacctgc
    39241 ttacggaaaa atggggcgct gaacccgatt cctactgggt agacgcttca gcctgccgcc
    39301 cgattactgc ggacgagatt accccaatcc atccggacgc gggcgataaa cttatccgcg
    39361 taaacatgaa ttatcagacg ctttcctgct tggagggagg aagggaagtc tacttctgtg
    39421 aggtttcctc cggcgggcct tccacgccgc ttggaaccac tctcatttgg cgcaagatga
    39481 tatcgacgca catgagcgcg ggcggattaa tagcctacga caccggtggt atcggctgga
    39541 cgacgctttt ccacggcgaa ggctccgcca tccatgccgc cttctggcac aacaactttg
    39601 gcaccgcgct ttcccacggc tgcatcaact gcttgccgga ggacgcgaag tggatctggc
    39661 gctggaccaa tccggtcgtg gattactacc cgggcgagct gaccgtcagc gacggcgggc
    39721 agaattccac acgcgtggaa gttttctact aaagcttacg cggccggatt attcaccctg
    39781 tctgacgcgg ggaatggata agcagtgatg aacgatgagg ttattcaggt taccctttac
    39841 tcgcgtccgt cgttggcgga acaaaacgag gctgtccttg agcggctggc agagctcgcg
    39901 caggaatacc cgcatcagct gacttgtatc gatattgacc ggaataatta tctcaaacgg
    39961 agcattggtg aacaaattcc gcgcctggag attggtccat atttcctgat tgaaagtttt
    40021 gatagcgggg atatccgcac ggcgctcaaa gaaacgcggg cgaagattga cgacgcgcac
    40081 acatccgcta atccatgggt cctgcggcaa tatgcccggc cgcgttcgtt tccgctcagg
    40141 gacagatttt ccttgtggtt cgcgcgccat tacgtcatgc tgtttactct gctggtattt
    40201 ctgtaccttg gcggagcttt tctggcaccg accttgatga agcttaagct tgaaccagca
    40261 gcgcgggcgt tgtacagctt ttaccgaccg gtttgccacc agttgggcta tcgctccttc
    40321 tatttatttg gcgggcagcc cttttatccg cgcgaattag ccgggctgga atcttaccag
    40381 accttctcgc aagccaccgg cattaacgaa ttggacaatc gcgcggcttc agaattcctt
    40441 ggcagcgaaa cccttgggta taaagttgcg ctttgtcagc gggacacggc aatttatacg
    40501 ctcatgctgc ttttcgggct cattttcggc gcgtcggggg gcagaatcaa agaaatacgc
    40561 tggtacatct gggttttgct tggcatcctt cccatcgcgc tggacggcgg tacccagatg
    40621 ctcagccaga tgggactggc tttcctaagc tggttcccgg cgcgggagag cactccgttt
    40681 ctgcgcgtgc tcaccggcgg actgttcggc ctctttaccg cctggcttgg ttatcctctg
    40741 gtggaaagca gcgtgcagat cagccggccg cagctggaaa ggcagtacgc gttgaacagc
    40801 ggggcagcgt tcgagcccgg gaggcgtcca tgattcgctg cgacgtcgac ggctgccgtt
    40861 attaccgctt cgaacaactg cccaaccagc aggttgacca tgccgtgttc acgcgcctgg
    40921 gaggcgcgag cgccggccct tacgccgccc tcaaccttgg cggaaccgta ggcgacaaac
    40981 ccgaggcagt cctggaaaac caccagcgct tgtttcaggt ttttggcagg ccgtatgaga
    41041 gccgctttga tgtctggcag gtacacggca cgaccatggt gtattccgac caacccagac
    41101 cgcgcgacca gaagcaccgg ccggcggacg gaattttcac cgataacccg gaggtcaccc
    41161 tgattatgcg cttcgccgac tgcgtcccgc tggtattttt cgatccaacc caccatgtgg
    41221 tggggctggt acacgcgggc tggcagggca cagcccaaaa aattggcgag ctggcggtga
    41281 attacatgcg cgagcgtttt aactccgatc cggctgcctt gtttgccggg attgggccgt
    41341 ccatttgcgg cagctgctac cagacaggcg aggaggtacg cgcgcgcttt ttacaggctt
    41401 acggcgcaga cgcgctggat tttttcatgg aacgggaggg ctcgctctac ctggatttgt
    41461 ggcgcgccaa tgcgtactgc ctgcagcggg ctggcgttca gcagctggaa caatgcggat
    41521 tgtgtaccgc cgaagacctg gatcaatggt attcctaccg caaggaaaaa ggcatcaccg
    41581 ggcgttttgc cgtggtaatc gggttgaaac atgctgcaca gtgaggatga aagtagattc
    41641 atcaggacga taggggccag gcttgccctg gtccgcgaga atatcgcgcg gagtgctgcg
    41701 aaagccggcc ggcgcgcgga ggcagtggag attgtgacca tctccaaacg ccagccagag
    41761 gtatacatcc aggccgctta cgcctgcggt ctgcgctgtt ttggtgagaa ctacgcggaa
    41821 gaagcgctcg tcaagatgaa caacctgagc ggtttgcccg acctgcagtg ggatatcgtc
    41881 ggccatatcc agagccggaa ggccaggctg atagccgacc gcgtcgcgcg cgtgcattcc
    41941 gtggacagcc tgaagctggc ggggctgctt tctcaactgc gtccagcgga catgccagcg
    42001 ttggatgtgc tccttgaagt gaacgtcagc ggggaagaaa gcaaaggcgg cttcccggcg
    42061 gtttctcaag ctgactggag cgcgctgctg ccggctgccg acgcggtcag ccgcctgccg
    42121 cggctgcgcc tgcgcggcct gatgtgcatg ccccccttgc agacggaaat ggaaaacaac
    42181 cggagctact tcagtaaaac gcgcgagctt ttggacttcc tgaaagcggc tctccccggg
    42241 ctggagctga gcgaactttc catgggaacc tcaagcgatt atcccgtcgc cgtcgaggaa
    42301 ggcgccacac tggttcgtct gggtgaagcc attcttgggc ctcgaccggt gaaagcagag
    42361 tagacaaata tgaccattgc gttgaccatc attcatacta tcgggcaggc gctcagcttg
    42421 ctcgtaatcc tttccaccgt gctgagttat ttcctcgcct gggatcatcc ggtgcgccgg
    42481 gggctggaca agattgttac ccccatgctg cggccgatcc gcagtctgat caaaccagtc
    42541 ggtggattgg atttttcgcc gtttgtgcta attctgctga tttacatgct ggagcagctg
    42601 ctctcatctt tggttcgagc gctcttccga tagagattct cagggcttat agagaattcg
    42661 cccgcaggtt ttacagcgca gcagggtggt ggctgagcgg actgcctgca tgtctcgtgg
    42721 ggaaagctca agcccgcaag cttcgcagcc gtcatccacg atggctgcca ccgctttccc
    42781 acccttggtc ttcagcaaat tttcatattg cgccacgatg tccgccggta aggtttgggt
    42841 aagggcttcg cgttgggagt taagattact catttccgtc aaaagcgttc cgcgttcacc
    42901 agccaggagt gaattttccg ctgctttttg gttcagcgct gccaggtagg ctgtttcagc
    42961 ttccacaagc gttttctggg cggtttcctg tgccattaaa gcttcaaatt gcgattcttc
    43021 cagcttctgg atgtagcgcc gcagggcttc gctttccgcc tggaggtcct gtaattcttt
    43081 gggattgcgg atttttcccc cgaagagctg aacctggttc aattcaagct tcaggcgctt
    43141 ttcagccgtt tccgccgcaa tttggttgac cgcctgctgc cttgccgtga ggtccgcctc
    43201 tgcctgcgtc ttttcccgca agctcgcttg caccgcctca tcggcgttca ggatagcttc
    43261 tatttgtgcc agtcgtttgg tgattttctg gcgctgggtg tcgagagtct gcagtcggaa
    43321 aaggctgaat gtaaggttca taggctcctc ggcttgggaa agaacacgat aaatgattat
    43381 ttattgtatc acttccagat tctggaactg cctgctttcg gacgcaaata gtattttcag
    43441 atggaattgc tgcagctttt tgtaatcccg gatgcttggt gggattaatt taggaaaaaa
    43501 tccgccgcgc agctggcgcg ctttggatgg gacagcgctc tgccgcgcgc aagcaaggtt
    43561 tccaccaagc gtgtctaccc ctgtggcggc gcgccgtttg cgttatgctg attatgttat
    43621 aatctgagcg ttaactacgc agttaatcca tgtgatggag atataacaat ttggctttca
    43681 atccgattaa ttggctgtta ggtttatttt cgctcgatat tggaattgac ctgggaacag
    43741 ccaatacgct tgttaatgta aaaggaaaag gaattgtcat caacgagcct tcgtgggtgg
    43801 caattgacaa acgtacgcgc gaacccttaa aaatcggcgc ggctgccaag gccatggtcg
    43861 gccgggcgcc tgccaacgtc gtgacgctgc gtcccctgcg cgacggcgtt atctccgaat
    43921 atgatatcac ccaggcgatg ctcgaattct ttatcggtcg ggcgcaccaa cacagcatcg
    43981 tgcccttccc acgcccgcgc gtggtggtcg gcatcccctc aggcgccact gaggttgaaa
    44041 aacgcgcggt atacgacgcg gcgatggcag ctggcgcgcg ggaagcctac atgattcacg
    44101 aacccgccgc ggccgcgatt ggcgcgggtt tgccggtgaa cgacggcaac ggcaacatga
    44161 ttgtggatat tggcggcggc accacagaag tcgcggtgat atccatgggg ggcatcgtca
    44221 tttcgcgctc cctgcgcgta gctggtgacg agctggacca ggacatcatc gaatacctgc
    44281 gcagcaagta caacctgttt attggtgagc agatggcgga aaaggtgaaa atcgctatcg
    44341 gttcagccta ccccttgaaa gatgaaaaaa ctgtggatgt gcgcggacgc aacctgatca
    44401 ccggcctgcc ggaagcgatt gaagtttcct ctgtcgaaat ccgcgaggcg ctttctgcgt
    44461 ctgtccaggt tatcatcgat acgatcaaag acgctttgga cgaatcccca cctgagatcc
    44521 tctcggacct gatggataac ggcatctgca tggctggcgg catgtcccaa ctgcaagggc
    44581 tggcggaacg cgtttccaat gaggtccggg tgcgcgtgtg ggttgcggaa gatccgatga
    44641 cctgcgtcgc gcgcggagcg ggcatcgtcc tggacgacct ggaagagtac gaacagctcc
    44701 tggttggcat agagcgggat ccgtacacga gcctgaccaa ggggtagttc gcctgcctcc
    44761 agcaggtctt atccaatcta caatgcgcaa tctgtttaca tccggtaaca tcagaaattt
    44821 catcatcctg ctggtggtgg ctggcatttt gctgatggca gtttcaggct acctgacccc
    44881 gctgttcagc ctgaccttca gcccgctgat ttcgactcag agctggcttt cccaacgcta
    44941 ccttgccatc cgggattttt tcacatcccc gcgggatatg gccagcctgc gcgagcgcaa
    45001 cgcccagctg gaaaacgaag tctcgcagct gcagagccag attgtggagc tgcaggaaaa
    45061 cctgagccag gctcaaatcc tgtataccct gctggacttc gcgcgcacaa acccgcagca
    45121 tgagtacact gccgcgaccg tgattggacg ggaaatcagc ccctattatc agtacatcat
    45181 catcgacaaa gggtccaacc agggcatcat gcacggcatg ccggtggtga cccagcaagg
    45241 tctggttggc cgcatcgatg cggtcatcgc gaacgcatcc cgcgtgcagc tgatcagcga
    45301 cgcggagtct gtcgtaaacg tccacttgca aaacgccaaa gtggaagggc aaattgtcgg
    45361 ttcaatcacc ggagaaatca ccctggaaat gattccacag gacgctgaag ttcaaattgg
    45421 cgacgtgctg ctgacctccg ggctgggcgg caattatccg cccaacatct tcgtcgggca
    45481 ggttttaagc atgcaaagcc agcaaaaccg tttgtttctc tccggttcgg tgcaatcgat
    45541 tgtggatttt tcttcgctca gcgcggtgtt gattattacc aattttgaag cagtggatat
    45601 tacgcccctg gtaccctgag agtcagcatg tttttctaca tttccagcag catcggcttt
    45661 ttgatcgcca tcatgctcca aatgggtgta atcagccgcg cggcactgct ctcgggcacg
    45721 gccgatgtgg tgctgctctt tctggccgcc tggagtttgc accaacgtca gaaatacagt
    45781 tggatgcttg tggtaatatt cgggttggtg ctcggcgcga tttcggcctc gcccacgctc
    45841 ttattcttaa ttgtttatct cagcgttttt ctggcagcca cccgactcca aatgagtttc
    45901 tggcatagtc cgcttctgtc gatgttttta ttggtctttt ttggcacttt tttggagcac
    45961 ggcgttttga tactggggtt gtttgtgcag ggagtttcca tcaacctgat tgaaatcttt
    46021 acccaggtgc ttctgcccag cgtcttgctc aatatgctgc taacaatacc aattcacgcg
    46081 ctggttcagg aggcattccg gaacgcagcg cccgcgggca cagaaatatg aataacactc
    46141 attttgaaaa accaaccatc aaccgctccc gcatccgggg ggttatcttt ctggtcgcga
    46201 tcgtggtttt ctattacatc ttccagctgt ttaactacca gattgtgaac ggcgcgctct
    46261 accgcgccca ggcggaggac aaccgcacct ccgaaatcag cgacccaacc caacgcggga
    46321 ttatttacga ccgaaacggt gttgtgctgg cgcgcaacga acccgcctac aacataacgg
    46381 tgactcccgc gaacctgccc gaatctgacg gagagctgcg gaagatttac tctgagcttt
    46441 cggaactgct caaaatcccg gtcagcaatg gtgtggtgga cgcggtcacc gcctccagct
    46501 ttaccccttg caacacgacg ctggggattg aacaaattgt gtacattgcc gataccaact
    46561 ggcctttcag cgctacccgc ttgagctgcg atgtctccaa ggaaatcgca atggcaatca
    46621 aggaaaaggc aatggattgg cctggtattg atgtggaaat tgaatccatc cgggtgtacc
    46681 cgaccggcaa cttgactgcg gaaatcgttg gcttcctggg cccggtgccg gaatcgatgg
    46741 tggattacta caccgggctc ggttttgtgg ccggccggga taaagtcgga tacgccggcg
    46801 tggagaacac gatgcaggat gttttggggg gcaccaacgg taagcgtgtg gttgaggtta
    46861 ccgttggcgg cgaggtggtg cgcaacctgg aagagccggt ggacccggtg cccgggcaaa
    46921 acatttacct caccattgac acccgcctgc agtctgccgc gcgggacgcc ctaatcatga
    46981 acctggatta ctggaaccgc tacctcggct acacattaag caccagcggc tcggtggtgg
    47041 cgctgaacgc caagacgggc gaagtgctgg cgatggtctc ataccctaat tatgagaata
    47101 accgtatgtc caaggtcatc cccggttatt actacaacca actgacgctc gaccaggcca
    47161 agccgcttgt caaccaggct atttctgccg aactgccgcc gggctcggtg tttaaattgg
    47221 tctcggcttt gggcattctg aacgaaggcg tcgtcacgcc ggaatacaca gtcgacgacc
    47281 ccggcacgat ttcgcttgtg cagaggttct accagaacga ccccggcagc ctgcagacct
    47341 attactgctg ggaccgcgcc gggcatgggc ccgtggatta cttgcacggg attgcctggt
    47401 cctgtaatgt gtattggtac aaggtgactg gcgggtatgg tactgaaatc ccgggcaatg
    47461 ggcttgggat ctggcgtttg ggtacgtacg cccgggcgct gggctacgga gctctgaccg
    47521 gaattgaact gcccggcgag accgacggat tgatcccaga ccccacctgg aaacgcctga
    47581 gccagggcga aaactgggca actggcgata cgtacctggc agctgtggga cagggctatg
    47641 ttttggcgac gccggtgcag gtggtgcatt ccatagccac catcgcgaac gatggaaagt
    47701 tgatgaaatt atcgattatc agcaaggtag aggccgcgga cggcacggtg acccggcaat
    47761 ttgaaccgac catgctgtgg gatattacca aagatccaaa aatagaagtc tacgacgaca
    47821 acattccaac cggtgagttg aaaaccgtgc agccctgggt aattgaattg gcaaaaaaag
    47881 gcatggaaat ggttacccaa cccggaggca ccgccgctct tgagttcaaa gacgatacga
    47941 acaaggttgc aggaaaaact ggcacggcgg agtactgcga cgattttgcg ctggcaaacc
    48001 ggctgtgcgg ggtcggcatt ggcggctggc cggcgcatgc ctggtttgtg ggctacgcgc
    48061 cgtatgacga cccggagatt gtggtggttg ccttcgcgta caacggtaag gaaggctcta
    48121 cgctggccgc gccgattgtc cgcaaggtga ttgagtccta tttcagcttg aaagccgcgg
    48181 acgcggataa tccgggatat taatagggac ggcagaatga gcaaacggac tgagctcttg
    48241 gtgcgcggaa tcaggggtgg agtgctggta atcctgccag atgccccctg gtatcagcag
    48301 cgtgatatgc tcatttcccg catccagagc caggagcgct tctttaaagg cggccgcatt
    48361 gcgttggacc tgggcgggac ggactggagc gaggaacatc tggataaatt actgagggac
    48421 ctggcggatg agggcatttg cctgcgcgcg gtcctttcca gctcccaaat tacgcttgtt
    48481 tcggcgcagg ttttcgggct gccgcccagt atcgaaggcg atgaaagacc gaaagcagca
    48541 gccccggaag ctgaagccgg accgcgggtg gtgtggatcg accgccctct ggaaaatgcc
    48601 gaaaaactga ttgtggatgg caacctggcg ctcttttctg acctgcctgc aggcgcggaa
    48661 gtcatttgct ccgggagcgc ggcgctttgg ggctgtctgg acggcatttt gcgggcaggc
    48721 tgcgctgggg accctgatag cgcggtgaac atcctgaaat tgggccttgg aagcgtttct
    48781 ttaaacggtg aagcggtcaa aattccgcgc aaattgcaca agaccgttgg aatcatgatc
    48841 agaagacagg acggggcgat taacgtcgtg ccgttggtga agaagtaagg cgtatgttta
    48901 caaccagaca gcagtggcag cattttgatt tccccttatt cgcggcagta ttatttctgt
    48961 caatttttgg agtggcattg atcagctcag cgatcgcggg taacccgact ctggcgaacc
    49021 atccccagcg ccagattgtg ttcctgatta tcgggctggc agcgctgttt atctttgcgg
    49081 gtctggacta taaaatctgg ctggtgctca cgcggccttt ctatatcgtc gtggcgcttt
    49141 tccttatcct ggtttttgtg agcggcgcgg ccaggttcgg cgctgcgcgc tggctgacca
    49201 ttggttcgga agctattcaa ccggctgagc tggctaaaat cgtcatcatc atggcgctgg
    49261 cgcagttctt tgccagcaag atatccagca tcaacgaatg gaagacggtc ggtcagagcc
    49321 tgttgctgac cggtgggatt gttatctgga tcatcctgca gccaaatttg agcacatcca
    49381 tcgtgattat ggtcatctgg ttcgccatgc tgtggattag cggctggaag accaaaaaca
    49441 tcctgattct tgccggggtg gcgattgtgt tactggggct gtttgccgcg ctggatttcc
    49501 catttctggc ggattaccag aaggcgcgta tcaccaactt catcaacccg gatgaaaacg
    49561 cgcgttacgg ggatacgtat aacgtagacc aggcgctcat cagcatcggg tccggcagct
    49621 ggtttgggca gggctacggg cagggctccc aggtgcagct gcgtttcctc aagatccggc
    49681 acaacgactt cattttctcc gtgctggcgc acgagttcgg ctttgtgggc actgtggcgg
    49741 tgctgctttt gctgttattt gtgattttcc gctgttttca gattgccgcg agggcgccgg
    49801 acgcctacgg cgcactgatt gcctacggct ttggggtgtt gatggctttc cagacgctgg
    49861 tgaacgtggg cgtgaatttg cgcctgctgc cggtcaccgg gctgaccttg ccgttcatca
    49921 gctacggcgg cagctcgctg gtgacgctgc tgatgggcat cgggctggtg cagagcgtgc
    49981 ggatgcgttc ggaacagagg ccggggtaat ggcgcttttg cccttgtaag gctgggaaat
    50041 tgctggtaaa ataggaattc gttagtgggg gctcgtctaa tggcaggaca gcagactctg
    50101 aatctgtatg tagaggttcg actcctttgc ccccagcctg agaggttgac cggaatggtc
    50161 agcctctttt ttatttaatc cgggacaaaa ttgttgattt ctggggattc ccgggaaaaa
    50221 gcaacacagg ataagtgctt tcctccttct tgctgtcaac cttatctcct gaagggaaca
    50281 accgcaatga aggattttgc ctggtaaaag cattcttctg cgccttagaa catcaatgta
    50341 gagacttgat aagtaacgca agaggtacag agaattattc ctcacctaat tcaggatttc
    50401 ctcaaccgca tccgtgtttc tagcatcaag tctctttacc aagattgcag cgatctcctt
    50461 aagggatgga gatgcatgat aggtagcgct gatgttgccc cgtcgtaggt cgggtggagc
    50521 ggcagcgcaa cccgacaagc tttggccagc gggtttgtga agcctcagcg caacccgaca
    50581 gaacgtagac gtaggttggg tggagcggca gcgcaacccg acagaacgag gtcgtaggtt
    50641 gggtggagcg gcagcgcaac ccgacagaac gtagacgtag gttgggtgga gcggcagcgc
    50701 aacccgacag aacgaggtcg taggttgggt ggagcagcag cgcaacccga caagctttgg
    50761 ccagcgggtt tgtgaagccg gcgttgggtt acgctcctgc gtcgctgcac ccaacctaca
    50821 aacttgagcg cggaggt
//
LOCUS       DAOQAD010000003        47975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_589_length_47975_cov_6.002045, whole genome shotgun sequence.
ACCESSION   DAOQAD010000003 DAOQAD010000000
VERSION     DAOQAD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 47975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47975
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_589_length_47975_cov_6.002045"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..254)
                     /locus_tag="PKL60_00480"
     tRNA            complement(180..254)
                     /locus_tag="PKL60_00480"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(218..220),aa:Val,seq:cac)
     gene            360..1742
                     /locus_tag="PKL60_00485"
     CDS             360..1742
                     /locus_tag="PKL60_00485"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNW94640.1"
                     /translation="MAIWWIRRDLRLTDNLALQAALEAGGAFVPVFVLDPALLARPAE
                     RRQAFLFAGLRALAENLHALGSGLLIRSGDPTEELPRLAQELGGLPIFAEEDASPYAR
                     ERDRLVGSRTALSLLPGAGAHPLGSVLKADGTPYTVFTPFSRAWKALPLPAANLPSPR
                     SLPPLPALPSAELPDLPAPASFPAGEQEAQRRLDAFLDGPIYDYKEDRDRMDLEGSSG
                     LSPYLRFGMLSARQAAQAARGASYRAPNESARAGAEIWLNELLWREFYLSILYHFPRV
                     LAESFVPAGKNIRWRQAPQDLAAWQSGRTGYPVVDAGMRQLASLGWMHNRARMITASF
                     LVKHLLINWQEGEAWFMRQLVDGDPASNNGGWQWTAGTGTDAAPYFRVFNPILQGLRF
                     DPLGVYVRRWVPELAGVPTRTIHTPWQMPAELKGAAGCRLGVDYPLPIVEHPLARERV
                     LSVYQQAFRR"
     gene            1853..2380
                     /locus_tag="PKL60_00490"
     CDS             1853..2380
                     /locus_tag="PKL60_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNW94641.1"
                     /translation="MSPRAALPLHLEYTLLGLLKQKPQYGYDILQNWDKNLGIIWKVK
                     PGRLYAGLNKLEQNGLISAQLESGENAPARKVFALTAQGEAVFSRWLRSPVPSPREFR
                     QEWFAKLLFFPELSAEERGVIYEEQIKAARRWLKAQSELNLTGSDFEAQVRRFRQMQI
                     QSILAFLIDTQQNQI"
     gene            2392..3474
                     /locus_tag="PKL60_00495"
     CDS             2392..3474
                     /locus_tag="PKL60_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW94642.1"
                     /translation="MKRLCTIFLLFTLLLSACSQAVQTQAPQTAVPIVITDALGREVK
                     LEKLPQRIAVTGKASLMILDALYMFPEAKERVVAFEDAVQGDLNFIKLLDPHYAEKAF
                     LKRDAGVEQFVSAEPDLVLLKSSQAEKYGAPIEALNIPVIYLDFETPEQYARDLAVLG
                     QIFGNDARAQELIQLYQGAVDEINQKIPATAAKPSVLVLQYADADGAVAFSVPPAGWM
                     QTQIVELAGGSPVWKDANPGKGWAQVTIEQVAVWDPEVILVISYDKDPSEVTATLKQD
                     AQWQQLRAVKDGKLFGFPKDVYSWDQPDTRWLLGLKWLAFRLHPAEFGQVDVLQSARE
                     FYQSAYGMDAAAYDANLLPITQGDID"
     gene            3471..4502
                     /locus_tag="PKL60_00500"
     CDS             3471..4502
                     /locus_tag="PKL60_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNW94643.1"
                     /translation="MNEDASLLKARPDRARKKWVWAVLVAALVLLLALSVFLGRYPQV
                     GFLRLSQLRENLLAQLLVVNVRLPRLLTALLLGMVLAASGTVFQMIFANPLVEPGFLG
                     VSQGAAFGAALAIVFFSASAALVQGMAALFAFAGLGFSYLLARRIRVGSWLLRLVLAG
                     IAVSALFSAGLGIVKYLADPLRQLPEITFWMLGGLASITWTKFLAVLPACFLGLTVIF
                     AFRWRLNLLALDETTAFSLGAAPRRERLVMMIAAVLATSAIISVAGMVGWIGLIIPHI
                     ARRLVGSDTRRNLPAAILLGGAFGVICDDLARMLMAGEIPLGILTSLLGAAIFILLMA
                     RGLGKEVRS"
     gene            4499..5281
                     /locus_tag="PKL60_00505"
     CDS             4499..5281
                     /locus_tag="PKL60_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW94644.1"
                     /translation="MSVNLLSFDHVAFGYSQARPIFSDLNYQLRRGTLSVILGPNGIG
                     KTTLLYMALGWLQPSAGQVLLENRPLQVYSRAELGRRVSLVPQTERQPYSYTVLEYVL
                     FGRAPHLALFGAPGENDELAARQALARAGIPELAGAYVDQLSGGEGQLVLIARALAQE
                     PRVLLLDEPSAHLDLGNKFRLMQTLSDLRRDGTTILMTTHDPEIALAVSDETVLVDGS
                     GSVTVGTPTDLLTADKLSQLYHLPVGALEIRSSHPGVTLRRF"
     gene            complement(5359..6693)
                     /locus_tag="PKL60_00510"
     CDS             complement(5359..6693)
                     /locus_tag="PKL60_00510"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW94645.1"
                     /translation="MNQLPVKKWVCNVCGFSSVGVEPPDECPQCGTDRNHFEEFRPAG
                     KRYLIIGAGIAGVSAADAIHQAEAEAKVVLLSDETELPYFRMNLTRYLAGELDDSKLD
                     LHSRAWYLENHIELHLNSKVVDIDPHAQRVFCADGRSYDYDTLILTTGASPFVPPFPG
                     ANLAGVQTLRTLHDANSILAVCTRPVNVVCIGGGLLGLEVAGAISRRGAQVSVLESLP
                     WLLPRQLDQAASRILQGKIEAMGIKVITGAKTKALLGAEHVESVELEDGARLPADLVV
                     ISAGVSANLELARKAGLAVNRGVLVDDTMRTSDPRIYAAGDDTEYQGRMYGLWVPARN
                     QGAIAGFSAAGKPARFAGDPPSARLKVLGIDLFSIGQITPSESSDLLLVDESGGSYAS
                     FLFRAGVLSGSILLGDASLAGPVKNAVESQMDFSADLTRGISAAEFKEMLNA"
     gene            6860..7231
                     /locus_tag="PKL60_00515"
     CDS             6860..7231
                     /locus_tag="PKL60_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94646.1"
                     /translation="MRSALSAFWLLAGCLAVIVLLGLFVARLRKAHGIKSAPGYLGGI
                     IWVIAMIGYAVLGRNEMADPLIFILVSSVIPLVYLVNILIILLSRRYTWKDPQDKRAA
                     GRFAGVILAVLLVWIIGLILV"
     gene            7316..8176
                     /locus_tag="PKL60_00520"
     CDS             7316..8176
                     /locus_tag="PKL60_00520"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNW94647.1"
                     /translation="MSNETTTRPKTSFADWILRLLKGVLVGIGFITPGLSGGALAVVF
                     GIYEPLMRWLGNLREKFLQNLIYFVPVGIGGILGVLAFSKVVEWAFGHYAAQFTWLFI
                     GFIAGTFPSLIKTSGKQGRKSWHVGLLAAVAVGTLFLMRWLETIRSVQMVQNFGSWLL
                     SGAMIGLGVVVPGMSPSNFLIYLGLYEPMASGISRLDFGVIIPLVLGGAACVLLFARL
                     VSWLFKKYYALMYHIILGIVVGSTLAIIPAGVHGLGMIAVCVLLFAAGGLVSFLLAKL
                     DEKYSRDAVE"
     gene            8307..9206
                     /locus_tag="PKL60_00525"
     CDS             8307..9206
                     /locus_tag="PKL60_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNW94648.1"
                     /translation="MQRDLENADWAVGLMQGRQSVRNFKEEPVPEEILYEVLAAGINS
                     ATGGNLQPYSIIVEKNRLRNQQLAQLCGGQSFIAQAPVNLIFLLDWHKLAVYSRKKDA
                     PFTCNKSFRHFLIAIEDIVCAAQSIESSAWLYGIGGCFVGTILECIPAVAEMYKLPML
                     SAPVLLLSLGYPKTLAVPRKKLDMDMVVFRGTYPALSAEKLCEAYDRKYSGMRFSLPA
                     DPHKRQALLDEFRQALRTTYNESKCDEIIRAADKAGSISEIQRRFGLHYHAAEMLSPD
                     ILEGLASQGIYPYYCLLDQDPEL"
     gene            9381..10325
                     /locus_tag="PKL60_00530"
     CDS             9381..10325
                     /locus_tag="PKL60_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW94649.1"
                     /translation="MSAVLEVHNLVKKYGENTAVNDVSFDIQEGEIFSLLGPNGAGKT
                     TTISILSTLLSPTAGDATIFGHSVTRDPMEVKRLIGVVPQEIALYDDMSALENLNFWG
                     EMYGLSGGALKTRVKELLEQIGLADRAKERIKTYSGGMKRRVNIAVGLMHQPRLLFMD
                     EPTVGIDPQSRRAILDSVKSLNAAGMTILYTTHYMEEAQELSDRVGIIDHGKLIALGT
                     QSELTAKVGEMDTLILHLAENEDSGHLAQALKTLSGVLQANPSDHTVALVTPVAEELL
                     APVVSRANELGIKIQSVDMREPNLETVFLHLTGRALRD"
     gene            10330..11580
                     /locus_tag="PKL60_00535"
     CDS             10330..11580
                     /locus_tag="PKL60_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW94650.1"
                     /translation="MNLRKLLLIALKDLRLIFRDPTGLVIMLLAPFALTLGMGALSGR
                     FSGSTQLGISQISVAIVNQDSGELGQALEDVFTSRELNELIAADILTDPAAARALVDE
                     DQIAAAIIVPEGFTASIFSPESGGDVPVVEFYENPTRPTSTGIARSILESFLNQVEIG
                     RVSGTLIVSQLLASGAIQPQQAAALGAEVGREVVGSTEDSPSIKLNANTVEGSGAKFD
                     LLAYIAPGMAMFFLMYTVTMGGRSLLVESRTGTLARMLISPTNPGFVLGGKGFGIFLT
                     SAAQLLILIGGTSLLFRLSWGDGTAVALLILAAAFGASGWGILMAAVLKTPGQVAITG
                     SAIALLFGIIGGSFFDLHMLPEWVRTIAKVTPNSWANEGFRILSMGGKLAAIQPHLTA
                     LMSMGALLYALGTILISRRGLVRK"
     gene            11589..12782
                     /locus_tag="PKL60_00540"
     CDS             11589..12782
                     /locus_tag="PKL60_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW94651.1"
                     /translation="MKKLFAIMKKDTIIRFTSPMVWMFFIILPVVFIFVISGGTTQNS
                     DPRIVLTVVDQAGSTLSGALVEELGHSSAVKPVLTEYDQALKDFEQLKVSAMLIIPAD
                     FSAGALTAGNASLELRKQPNTLNGMAVEQAVQLAASRIASLADAARVSTERAAEFQPF
                     ADDAARQAFYDSAFQQAQILLAEAPDRLQSVVGSTTDPIEYDAKANSTAGQMITFVFI
                     PLVSLSVMFAYERANGTLRRLLVTPTSKATFLGGTILGQGLTALVQMTILIVFGILVM
                     KMKWGQSPAGLAMVMVSFTLAASALGTMLGTFVKSEGQANGLSLMIGMLMAMLGGCWY
                     PIELFPAALRNAAQALPTYWAMQGFLDIAVRGQGPAGVLQECAVLLGFALVFFAVGIT
                     RFKYE"
     assembly_gap    13200..13299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13335..13434
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13767..16196)
                     /locus_tag="PKL60_00545"
     CDS             complement(13767..16196)
                     /locus_tag="PKL60_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNW94652.1"
                     /translation="MSDNLPEVNVQRRRKGAKPTSQAQAPKRPSSGGAYSGEGSASSG
                     GSSGIPGGNVLRTTGGKTGCGSIGLFIIAAIIYFLFFRGDGSQVAEPGYDYPTEEPQT
                     VVQQPTALPVTRTPRPTSASGDTWTVMVYQDADDQALEEDVFIDMNEMERIGSTDNVR
                     IITQMDRFRGGYSGTDNWFSARRYLVLYDDDLYTIGSDMLMDLGEVDMADGNSLVDFV
                     TWTVQNYPADRYMLIMSDHGMGWPGGWSDPAPATPDGGSAPIINALQQDSIYLNELDQ
                     ALAQIQANTGIEKFDLIGMDACLMSQLEVYTALQPYAHYAVASEETEPGLGWAYSAFI
                     SLLIDNPGIDTADLATNIVETYIQQDERVTDDQARNDFLAQNSSSGGFFSSYRMSAEQ
                     LASQLEQNVTLTAVNLDNLPALNQAFNGLVYDMQSLNQQVVASARSYAQSFTSIFGSQ
                     VPPSYIDLGHFIQLLYKKSGGDAAIRQSANDVINALNAVLVAEKHGPNKPGATGIAIY
                     FPNSQLYSNAATGMRSYTMLADRFSRASLWDDFLVFHYSNRSFKESAAEAVTPPSSAV
                     TRAPGGGQISVSPIQASASSVTPGGSIKLSADITGTNIGYIYLFTGLYDEASNSIFIA
                     DTDYLESPEQDSLNGVYYPVWPEGGAFRMNFDFEPLLFSITDGSQSALAMFNPVSYGA
                     SAEQAVYAVRGTYTFADTGEQRTAELYFKDGQLFQVFGFKGGDAAQQPSQITPAAGDT
                     FTIAYRWLNLDSSGKVSGQTVTEGDTLTFGTSAFSWEQVYAPDGQYLVGFLVADLDGA
                     ITPAYTQITVR"
     gene            16377..16733
                     /locus_tag="PKL60_00550"
     CDS             16377..16733
                     /locus_tag="PKL60_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNW94653.1"
                     /translation="MSVKRIEEVPAELVPDAFGVSKQVLISAGEAPNFAMRCFTIQPG
                     GSMPLHTNLVEHEQYVLGGNAKVVIGEESCLVGPGSVVFIPAGAAHAYVNTGSEPFRF
                     LCLIPNREDKTSFVSS"
     gene            16834..17694
                     /locus_tag="PKL60_00555"
     CDS             16834..17694
                     /locus_tag="PKL60_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNW94654.1"
                     /translation="MISQLPPLVNVLLLSLIASLGTGLGGLIAVIRRPGKRSYGFLMG
                     ITAGVMICLSFLELVNEAWDLGGPITATVGFGLGASFMFLLDYLAPHIRFGEVEYHGA
                     HATEAEANDCEDFRPRRRFRFARLRAQGAQKKPVDQKLISSGILLAIGITLHNLPEGI
                     AVGAGYLHSPQFGLFVALAILLHNIPEGLATALPLCKGGVRRRDALRVAFLSGFAEPV
                     GALLAGLFLVNFTNFIPGALAFAGGVMVFITLDELIPSAREYGHVHWTSLGIILGSLF
                     VFVLSALLGV"
     assembly_gap    17753..17852
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17867..18031)
                     /locus_tag="PKL60_00560"
     CDS             complement(17867..18031)
                     /locus_tag="PKL60_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94655.1"
                     /translation="MWEAFIRILPGNSLTFELFKPEMDEAFLNPAGSAAQYRLSLSSA
                     AITHLPLLFR"
     gene            complement(18243..19664)
                     /locus_tag="PKL60_00565"
     CDS             complement(18243..19664)
                     /locus_tag="PKL60_00565"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HNW94656.1"
                     /translation="MTNATHKENPGRNAPKHSWSAAWALLLALCLAPYLALSAFARPQ
                     ADDYCSSAQFRSLGFWQAQTNAFTSWTNRYATMFFTGILDRFGLWGLRVLPILLILGL
                     AASVYLLLRQFLLRLNAGQPRLNLLICSLALIEITLSALPNLYQSVYWRAGSLAYTLP
                     VIALNLLLAGLLRPRKNKPAWYLQVLFALGAFLAAGFSTVNAAVQFTALGLAFCALLW
                     RKRLGAHERGLWVSALVGSLLGLLVLALQPGAHLRLAQMPPMPGLGRLIYLTLRHTAA
                     LVYHSLLDFLPSRLTALLLGLLFGLSVREPRLPSPKKLIPPLLLAALAAFLLLAAFCA
                     PGALAQSAYPEARSQSVAAFLISALLLGAGALSGAWLRALLSEQSSRMLHILLNLGAL
                     VSLFFALQQLPAHFAEFRRHAAAWDEREVLILQQKEAGLRNLLVPGVDSLAGLMELSP
                     DPSQWVNGCVASYYQLDSIRSTE"
     gene            20487..21341
                     /gene="panB"
                     /locus_tag="PKL60_00570"
     CDS             20487..21341
                     /gene="panB"
                     /locus_tag="PKL60_00570"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNW94657.1"
                     /translation="MTEQRKKLTLQGLFKKAAEGKPITWLTCYDYPTAYLQEQAGVDM
                     ILVGDSLGMTMLGYDSTLPVTMDDMIRHSAAVRRGAPNTWLVGDMPYMSYQPSNETAV
                     RSAGRFMAEAGCDSIKLEGGAAVCERIRAIADAGIPVIGHLGLTPQSVSSLGGFRLQG
                     KSAATAKKILDDAKALEEAGAFMILLELVPDRLCKLVTERAKNCLIMSLGSGLDAHGQ
                     LLIYHDAFALYPKFKPRMAKVFADAGSVIRQGLEAYVKEVSEKTFPAPENWFGMSDDE
                     YNELLSML"
     gene            21352..22614
                     /locus_tag="PKL60_00575"
     CDS             21352..22614
                     /locus_tag="PKL60_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94658.1"
                     /translation="MSDLRETLKIPAGNLEAVNSVLLDPDSRVVNAFLDVVARYGTPE
                     EINRKHRESRRMEVLFEKIRQKAPQYLEDLHWLIDQRSRGSFVRLEDYRKGILGEDFD
                     PKCINESCAVTLEVSALQYFPWVRPMLEQAIANRTLVPGRFIAVRKMKESEMDGDLPA
                     IVAALDIIGASFVETLDTKGTDGSNPHLGGPATLTGYFGGIGEPNGHALRWLDEFLYY
                     YTNYGVQHVLNFNAGTILLGFMLYRLGVDIQFKISVYFGADNPYSALWIMTMAKLFSR
                     PDGTSPLIGFNWSNSINNETLELSSEFRKALGFERVIRFEHHITETQKSIVIQPYNRR
                     QELLEVAERVPNIAAKHEGGDPEIDAAREHPSDILDYFRDKAEVIASGDWEHLQVNFL
                     DKVAACNATARALTERKLGFVAARNLHI"
     gene            complement(22761..23039)
                     /locus_tag="PKL60_00580"
     CDS             complement(22761..23039)
                     /locus_tag="PKL60_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94659.1"
                     /translation="MSPRKQFTQEEAKALAEQLGVDWTHFNVEQFTMGLNVELEHGTR
                     NQLTNVTSDDPLMTAKIALAHLTEFPDYYTRLDRLETEADNYWKDKKK"
     gene            complement(23127..24083)
                     /locus_tag="PKL60_00585"
     CDS             complement(23127..24083)
                     /locus_tag="PKL60_00585"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNW94660.1"
                     /translation="MKPKIKKLIPAAFALLLAAAAFAAADRYLIRHVEIGDVNAYASA
                     AVSTQALPEAASSAPESSTQTSEPVWDDWSYHSQTSEITITKTTLGSGADTITYFVAD
                     VVLKDGANILSAFAENKFGTNIIAATSEIAAANDAVFAINGDYYGFREDGILIRNGVI
                     YRDVPARVGLALYADGNMQVYDETTTNAQALLDSGVINTLSFGPALLDEGEVVAALES
                     VEVDHNFGNHSIQGAQPRTALGIIAPNHYVFVVVDGRSSGYSRGMTLTELADLFQELG
                     CTAAYNLDGGGSSTMVFMGRVVNQPSGRNGERGTSDILYISA"
     gene            24325..24858
                     /locus_tag="PKL60_00590"
     CDS             24325..24858
                     /locus_tag="PKL60_00590"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNW94661.1"
                     /translation="MYLLFALSALVEGPLTILTGGAGIARGFLQPLPIYFAVVAGNLL
                     GDIGWYSLGRFSKPGWVYQIAPKLGISAEKVSGLQEIVRKHTSKLIFAAKFSAGLPIP
                     TLIAVGLSRVPKRQWAAAWISGELLKSAIIMLAGYLFSAAIQTASRTMQVVLWGVTAL
                     LALAGIFWFRRRKHTRA"
     gene            24887..25579
                     /locus_tag="PKL60_00595"
     CDS             24887..25579
                     /locus_tag="PKL60_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW94662.1"
                     /translation="MKEEQTLPNVLVIIPAYNEAERLGPVAEAAGSLLPVLVVDDGSG
                     DETAEIGRQAGAQVLEQTPNQGKGAALRAGFRCALARGYEAVITLDADGQHDPAEIPA
                     FLQVFQQSGADLVIGRRDFSRMPFIRRLSNTVGTRLFSRALGQTVPDNQSGYRLISRR
                     LMEASLNCAERGFAFEVEMIVECVRNHFQLAWVPIRTIYAGEGSHISPLKHAAEFVRV
                     SYRTRRSLRRAD"
     gene            complement(25653..26525)
                     /locus_tag="PKL60_00600"
     CDS             complement(25653..26525)
                     /locus_tag="PKL60_00600"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNW94663.1"
                     /translation="MELRQLEYFQAFGRLESMTRAAEQLRVSQPSISVAIKKLEEELG
                     ISLVDRSHKKGTLTREGQIFLKRVEHVLGDLHDAVVEISDIQRPLGGALRIGITPIMG
                     ALMFPRFLSAYQKLHPDVKVSIVEEGSLLIREQLENSELDIALMITSDLPSSLEVLPV
                     KSGQIYVCLSRTNPLSRLERVPFDQLQEFEFILFKEDTFIRQRILAECARFHFSPRIL
                     FSSSQIGTILELVEQGMGITFFIQDIVPQRPDLVVLPLAEPLSLDVGLAWKKERYLSI
                     AAQNFIESSQRIRG"
     gene            26682..29351
                     /locus_tag="PKL60_00605"
     CDS             26682..29351
                     /locus_tag="PKL60_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNW94664.1"
                     /translation="MKEITLTINGEKHSLSVPPDLVLIDLLRRDLRLTGTKQSCDRKG
                     QCGACTVIVNGKAVRSCLTRVANLDGAEVITVEGLGTPANPHMIQEAFALAGAIQCGF
                     CTPGFIMSAKGLLDRNPDPSVEEIKKAFTHNLCRCTGYKKIIEAVQLAGRFMRGETTP
                     EAERARIGRETVGVSHVRPSALLKACGVAKFNDDIPLPEDALEIAVVRSTEYHANILS
                     IDTSEAEKMPGVVGFLRASDIKGTNRLRQVQPDQPVLCEDRVRLLGDPIIAVAANTRE
                     QARAAAAAVKVQYEPLPVQMTTRESLAPGAFVIHAHAPDNICCTQPLIKGDADAALAE
                     AAYIYEGEYSTQTNHQAPLEPEVSSAYFEGEGDARELVVVGRSINIHFHLNQLKEALG
                     HTKMRYQEAYSGGQFGIKVWIITEAIAGAAALHFNRPVRYAPTMAESMLITTKRHAYP
                     SIKIKLTADKEGHFTALFCDFTMDKGAYTVNGPVILGRAIMMLTSSYNFPNVKAFGRN
                     VYTNNGAGGSARGAGPPQVAFALESAVDMLAEKIGIDPLEFRRMNSLLPGQTKGVGMV
                     ADEWCFPEVCDSIEDAYRRARQKAESFNQTSPVIKRGVGIAAHSFGIGGAGDSAKMAI
                     ELNPDDSVTIYAAVADPGEGNDAMLTQIAAHQLGLPLEKIHLYTRDTDKTVLMGPAAG
                     SRMTFMAGNTLLNAIGKLQAAMAEVGAADYADLVAAGKPTRYEGFHKNDVPAGLDKHT
                     GQGYAQVSECHNIQMAEVEVNTETGAARVLKMTVAVDAGTIINPQAFEGQLEGGMDQG
                     VGYALREEYILGKTTDYVSFKFPTIKDSFDVAAIISKTPRSNGPLGATGVGEMTMVST
                     APAVTNAIYHACGARVYALPATPEKVLKALAAK"
     gene            29473..30783
                     /locus_tag="PKL60_00610"
     CDS             29473..30783
                     /locus_tag="PKL60_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNW94665.1"
                     /translation="METTGPIENFRADFPALHQTRNSLPPVYFDNACTTLVPQPVIDE
                     LTAYYTEYPACGGRRSHHWFASEVTRRVNAARERLAGFIKAESSSEIIFTLNTTQAIN
                     MVALGLPFKAGDVVLLSGEEHNSNLLPWLRLQQKGAVRVEHLSAAESEEFDLAAFEER
                     LSRAPVRLVSLAWTSNVTGSSLPAQEIIAAAHRHGALVLLDGAQTVPHQAVDVRSLDV
                     DFLAFSLHKMCGPRGVGVLYARNEFISAPSGSPNALEAVLLGGGNAINSTFQGYQLLE
                     GPERFEPGIPDYPGQIAAGAAVDYLEHVGMERIRKQEESLNSYLSARMQERYGHLGWL
                     EILGPLEPERRGGILSFSVRRPNAVGIAEELDRAANIMIRDGVFCAHSYFNERYGEAW
                     LHPKTHREHRMVYRVSLYFYNTRAECEFFLNALDAVFQERGYLE"
     gene            30792..31256
                     /locus_tag="PKL60_00615"
     CDS             30792..31256
                     /locus_tag="PKL60_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNW94666.1"
                     /translation="MDEIVIRQYRQLLRQDFPHSGELKNPSVFVEAVGENLIHCGNTG
                     NFMQLYLKIEQERISVMTYLCSCEPTANVAVEALCEMAQGKTLGEAEKISCEDLLQAV
                     GSKDEELGLKSAGLLALLGQAIREYQQGNAGGGGQASDPGREHISWDNTLSL"
     gene            complement(31510..33906)
                     /locus_tag="PKL60_00620"
     CDS             complement(31510..33906)
                     /locus_tag="PKL60_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNW94667.1"
                     /translation="MQPIPDLINQLTLEEKAALCSGQNFWFLKALERLDIPGIMLTDG
                     PHGLRKQADSPDHVGLNQSVPATCFPTASALAATWNRDLVFKVGQALAEECLQEGVSV
                     ILGPGANIKRSPLCGRNFEYFSEDPFLTGEIAKSHILGVQSLGVGSSLKHFALNNQES
                     RRMTANSVVDPRAMREIYLSGFERAVKDAQPWTVMCSYNRLNGTYACENKTLLSDILR
                     EDWGFEGLVVTDWGAMNEVVAAVRAGTDLEMPGVANGNTEKLIAAVHSGELDEAVLDQ
                     AVGRVLSLIQKSSESYQPGFSYDPHAHHALARQAASEGAVLLKNEGGLLPLSSGGKVA
                     LIGRFAKTPRYQGAGSSLITPTRLENLYEELGKLIGEERLLYTPGYTEQGQADDALLR
                     EALEFASRAETVILCVGLTDMDEVEGLDREHMRLPAGHDALIAAVCAVHPRCAVVLSN
                     GSPVEMPWLAAAPAVLEAYLGGQAGAGAVADILTGRVNPSGKLAETFPIRLADTPAQP
                     YPGGPQTVEYRESIYVGYRYYDAVGAQVLFPFGHGLSYTAFEYSGLTLRISGEHVIAS
                     FSLKNRGSRPGKETAQLYVRDLESSVFRPEKELKGFLKVELQPGEERQLEMELDPRAF
                     AFYDAESGQWATEGGDFEILIGASSRDIRLRGLIQLPGSSLPRAPRTEGLEPYFRPTQ
                     AVGFPRESFEALMGSPLPGNTPTKKGQFSLNTPLADMKSTLVGAYLFRSFEKGMQKMN
                     GPEQDTPTSKMMNAMLQEMPLRTALMMGGGVSRGKLLALVTMMNGRFFRGLRDFLRAR
                     "
     gene            complement(34065..34322)
                     /locus_tag="PKL60_00625"
     CDS             complement(34065..34322)
                     /locus_tag="PKL60_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNW94668.1"
                     /translation="MHQQLLKYCDNKKLVAIMEDIRGLIEWARGTPVGQPRSYDVAVR
                     EHMQILTYLKSNDREKAVQAMRSHLTSSCERTLGYWRGRSQ"
     gene            complement(34340..34915)
                     /locus_tag="PKL60_00630"
     CDS             complement(34340..34915)
                     /locus_tag="PKL60_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNW94669.1"
                     /translation="MNTDQTNEHRLLQEEAETFLNVLQARFEHNPRRHPGLAWSDVRV
                     RLASEPGKMWSLQAMEASGGEPDAVGKDAESGEIIFADCSAETPLGRRNLCYDRAALE
                     ARKTARPAGSALEMAAEMGISLLNEDEYSALQTLGEFDLKTSSWVLTPEGVRKLGGAL
                     FGDRRYGRVFFYHNSAESYYGVRGFRGLLRV"
     gene            35702..37804
                     /locus_tag="PKL60_00635"
     CDS             35702..37804
                     /locus_tag="PKL60_00635"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNW94670.1"
                     /translation="MQSIARTKNRNFLQNGAAAILKNRWFYLGLTVLITSIVLIFTLE
                     MQLYTVSLVSGLLGIALAAYFILRITLRPVTTQEMVEVATDIRTGARTYLRRQIKTIL
                     LVTPFFAALLFWILDWKAALTAVFGVLTSLMASYIGMSVSVRTNLQTADIAREGKREP
                     FRTALFGGSVMGLCITGLSLIVLSVLFSIFRDPNALIGFAFGGGLAALFAQIGGGIFT
                     KSADIGADLVGKIEENIPEDDPRNAAVIADLVGDNVGDCAGRGADLFQTFSDDLVTGS
                     IVAVAMASVYGPTAIFFPFLLQSVGVFASAAGIFASREWKPGMNPSTQFNVGLFVSSV
                     LSIIGALGMSMLLLNDWIIGIAATLGVLITTVASLSTRYYAGMEGKPVRNIAKASNRG
                     AALNLITGISYGLQSPVLPVIVLMGVVIGVYTLSGNSLLALVAVNIGTDLLITFIMAA
                     DAFGPIVDNAAGIAGMSRAPQHVIDKLSHLDSVGNTMKAVTKAYAMISGTITAFVIFL
                     TFYKLAGIDAIDVTTPFNLSFLFIGVCLPFLISSLVIGSTAKTAESMVDAVRDQFQQN
                     PDILKGLAKPNYSQCIDIATSNALHEMILPSAISILVPIGIGFAFGMNALGAVLVGSV
                     ACSALLGPFFNNTGTAFDNAKKMIENLEHSRGTPEHIAAVIGDTVGDSLKDVAGPSTL
                     IFMKLIGMASLLIVPLIK"
     gene            37847..38587
                     /gene="deoC"
                     /locus_tag="PKL60_00640"
     CDS             37847..38587
                     /gene="deoC"
                     /locus_tag="PKL60_00640"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNW94671.1"
                     /translation="MNPTKKASEMSVRELAAYIDQSVLKPEFTQAEIHRYIQEGVDFG
                     CATVCINPASLDIAAELTRGTDTKICVVCDFPFGLSTTRSKVMQAEEYCRRGDVAELD
                     VVANYGWIRSGLFAEVEADIRAVVDACHRCGTAVKVIFETDALTLDEVRQACEAGIAA
                     GADFLKTSTGFFTGGKSDGATSEVIALMMETARGRCKIKGSGGIRDQRHFFELIDMGI
                     DRMGIGYRSTPLVLGVSAEEARRLRGLG"
     gene            39144..39854
                     /locus_tag="PKL60_00645"
     CDS             39144..39854
                     /locus_tag="PKL60_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNW94672.1"
                     /translation="MKLTRRQETFILCLLDLFREYKRPIHYSTLAERVGVSPFTAYDM
                     LRLLEEKGFVTSEYRIEPGKSTVGRSEIVFLPTQLTYNRFAELANGTDPDDWEIAKSQ
                     ILKRILAGGIHDRELADELIARIPTDEPETVRYCFEVIALLFLRLGKNSGRKVLIERM
                     PQILAWKESFSKSGLIILGGFILGLLATDNPLILEADQPIFEYVQRYQSLVSEMEPRL
                     ANHLASTIYDMFSSVLKA"
     gene            39881..40828
                     /locus_tag="PKL60_00650"
     CDS             39881..40828
                     /locus_tag="PKL60_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW94673.1"
                     /translation="MTNTIEAIHLTKRYPSQPKQSRPALDNYDLQVKAGQLYGLVGPD
                     GAGKTTLLRILSTVLSPTSGSAHIEGFDIVKQSAQVRSRIGYMPQAFSLYPDLSVLEN
                     LRFFADMNGVPSEKQKQRIPELLEFARLTEFTSRRSENLSGGMRKKLALACAMIHEPE
                     ILLLDEPTTGVDPVSRRELWQLLAKVIERGVTIVVSTPYMDEAERCDVVSVIYQGKSL
                     ISGSPMEMAQHMPFKIVEVKARPRGTLRQVAGNLDGVIQWRAVGDRLRLSVANSTAVQ
                     ENLKAALTKAGAEITILREARITMEDVFIYLVEKQETPV"
     gene            40825..41778
                     /locus_tag="PKL60_00655"
     CDS             40825..41778
                     /locus_tag="PKL60_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW94674.1"
                     /translation="MNRENSVEVHQLTRNFGEFTAVDHISFDIPKGEIFGLLGPNGAG
                     KTTTIRMLCGLLKPTEGEARVLGFDVADQSEQVKRRIGYMSQKFSLYNDLTVYENLWF
                     YANLYGIPSERVKMRITELIEMAGLRGHEKQLTHNLSGAWRQRLALACAIIHEPPMLF
                     LDEPTAGVDPVSRREFWEMIYTMAGKGVSVLATTHYMDEAEFCNVIGMMYHSRLIALN
                     DPDTLKINMQGYLYEVDCDAPGRAEQILNSLVEVEDVAAYGVLLHVLLSKDSDKNLIL
                     RKLSENQIQVTRMEKVQPSLEDVFVSLVNQENRQQVRAEIE"
     gene            41810..42925
                     /locus_tag="PKL60_00660"
     CDS             41810..42925
                     /locus_tag="PKL60_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW94675.1"
                     /translation="MFTIVRKEWLHIIRDPRTLGLVIIMPVTMLFLLGYAVANDVTDI
                     PMVVADLSQTESSRTLVDKLTVSGFFKYTYSAQSENEILQLLDESAVKVGLYIPENFG
                     RNVSTGETGTIQFYIDGSNPTVAQTAQLAAETISQSMSQEILIKRLERSGSGMQLNLP
                     VDARMRYLYNPDMKRMNFMIPGLVAIILQIQTLLLTAFAIVREREQGTLEQLIVTPVR
                     SWELMLGKILPFVVVAFVNVIMTVSVGAIWFGVRVAGSIILLGILSLIFLLGSLGLGV
                     FISNISKTQMQAMYMASFIMMPSFILAGLLFPRENMPWLAYYAGYLLPVTYFLEIVRG
                     IILKGAGFSTLWMWIWPMAVFSIIVFFASVLMFRKRL"
     gene            42967..44256
                     /locus_tag="PKL60_00665"
     CDS             42967..44256
                     /locus_tag="PKL60_00665"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNW94676.1"
                     /translation="MKRQAYLFPSLFLSVAILLTGCQAINGNAANELKASGIISATEI
                     NIASQLGGTVVSIEVREGSQVKKGDVLFRLDDALLQAQRNQASAAVNAAEKAVNSARI
                     QYDIAVNSARLQEQQNRVNSWKIPQPKEFQLPTWYFDKGEKINAAKAEVDAAAADLKI
                     EKENLEKVLADNTSQEFLSAEKRLAEAQTAFIIADQVLTQADAAQDKEDISNYANDLY
                     DMAKSELSSAQTEYKRLVTTQAAAEVLEARARVRVAQERLDRAVDYRNSLESGDQSLM
                     VRAALAGVQQAEAVLTQAQAAQALIDIQLEKSIIKSPADGIVLTRNLEEGEMLSPGSV
                     AIIIGQLQEVSLTVYIPETEYGKIKLGDSVSIAVDSFPGEKFSGIVSYISDQAEFTPR
                     NVQTIDGRKTTVYAVKITVPNSNLELKPGMPADGTFN"
     gene            44258..45130
                     /locus_tag="PKL60_00670"
     CDS             44258..45130
                     /locus_tag="PKL60_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNW94677.1"
                     /translation="MKTQNSTVVVTGSAAQVQPEIAAQLGIVILPLIVVVDGKEHLDG
                     VDIFPGELYQKMRTQKIEVKTAAPNVGQYYECFKRIINQQGSDILCLTLSSQLSSDYN
                     AAVEAAKMLSEENQENRVTVFDSLRAAAPQGLLAVEAAKRLHAGETLEDTVQYLQNAR
                     YKSGLIAALDTLEYLAKGGRIGKAAYLVGSALQIIPILTINNEGIVAPSTVIRKKRNI
                     IPTFLSTLARKTAGYKRLSLSVMHADSVDQVETLRHVLREQYPTLEIPISEFTPVMGA
                     HTGPGLLGLGYFFE"
     gene            45157..45843
                     /locus_tag="PKL60_00675"
     CDS             45157..45843
                     /locus_tag="PKL60_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNW94678.1"
                     /translation="MLEKISQSMPAVTIFSLLVFAYLLGSIPSGYLAGKWLKGIDLRE
                     YGSGTVSGSMVWEHVAKWAVMPVGLFDIFKGALPTWLGLRLGLGEVPAMLAGGFAMVG
                     HNWPVYLNFQGGRGFSPFLGELLVLFPWGVIIILAGLVLGNFFHSAAVPLFAVILLPI
                     FTTWMGGPSIILWCSMMMVLITLIKRVEANQRPLPANPTERREVILRRIFLDRDIQDH
                     QAWIRREPKE"
     gene            45858..46301
                     /locus_tag="PKL60_00680"
     CDS             45858..46301
                     /locus_tag="PKL60_00680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNW94679.1"
                     /translation="MDFLPEHGSCFVCGKSNPKSIGIAWQLLAGNHILASFVFSDSQQ
                     GPPGYVHGGASAAVMDEAMGLAVFYSGYRAVTANMSVDYRKPIPLGQRVTIQAEMSGK
                     TERRILARGEILLPDGSVAVSATGMYAEAKLFFDKIAGQLLEPKE"
     gene            46298..46963
                     /locus_tag="PKL60_00685"
     CDS             46298..46963
                     /locus_tag="PKL60_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94680.1"
                     /translation="MKRLVSKIIWVAVFSIAMAYLEAAIVVYLRRLFGIGDMILQVPP
                     FDQQIAAIELGRELATLMMLLAIGWIAGHNIQARLSYTLIAFGVWDIFYYLWLNVFIG
                     WPKSFLDPDLLFLIPLPWWGPVLSPVLISILMITGGVAVLKENETKAPIHFPLSFWIL
                     LIPGVLLMLYAFMADAIRVLPASAAVLNSLRPSQFNWPVYLIGLVLAIVSVLQIIKRK
                     NAV"
     gene            47001..47480
                     /locus_tag="PKL60_00690"
     CDS             47001..47480
                     /locus_tag="PKL60_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNW94681.1"
                     /translation="MKIRYLGHSCVEIVGRHHILIDPDFTREPLPDVEYICVSHAHKD
                     HLGKVAQIPTGIVLASQDVCEIALKMGVPSSRLQPVSVGEHIDNIEILPGFSVVKDPV
                     YFIFRLLFRFSIPDPGGTPLSFFLFRMMQTYSMLVMLINFRWRFIRIFYVFHGALRS"
BASE COUNT        10029 a        12873 c        13954 g        10819 t
ORIGIN      
        1 ctgctggtca ggagaccagc aggagcggtt gacatgggcg cgaccagccg caggcggcga
       61 ggagccgggc gcgatcccct gaaggggact taccaccgaa cctttcttac gccctccccg
      121 ggtgcacgga tgggattata aaaaactgct ggtcaggaga ccagcaggag cggttgacat
      181 gggcgcgaga gggctcgaac ctccgaacct cacggatgtg aaccgtgcgc tctaaccaac
      241 tgagctacgc gcccgaagtg taaagattat agcaaacccc cgcgcatttg tccacccgaa
      301 atagaatgcc cctcgggtaa aattaacaca cgcagtaagc tcaacctgaa aggacgccca
      361 tggccatctg gtggattcgc cgggatttac gactgacgga caaccttgcc ctccaagccg
      421 ctctcgaagc cggcggcgcc tttgtgccgg tttttgtcct ggaccccgcc ttgcttgcca
      481 ggccggccga gcgccggcag gcctttcttt tcgccggttt gcgcgcgctg gcggaaaacc
      541 tgcacgcgct cggctccggg ctgcttatcc gcagcggcga cccgaccgag gagctgccgc
      601 gcctggcgca ggaattgggc gggctgccta ttttcgcgga agaggacgcc tcgccttacg
      661 cccgcgaacg cgaccgtttg gtcggcagcc gcactgcgct cagcttgctc cccggcgcag
      721 gcgcgcaccc gctcgggtct gtgctcaaag ccgacggcac gccctacacc gtctttaccc
      781 ccttcagccg cgcctggaaa gcccttccgc tgcctgccgc caacttgccc agcccccgtt
      841 ccctgccccc gctgcccgct ctcccctccg cggaactgcc ggacttgcct gcccctgcca
      901 gcttcccggc cggcgaacag gaagctcagc gccgcctgga cgcgttcctg gacggaccaa
      961 tctacgacta caaagaggac cgcgaccgca tggacctgga gggcagctcc gggctttccc
     1021 cttatctgcg ttttggcatg ctttccgccc ggcaggccgc ccaggccgcg cgcggggcaa
     1081 gctatcgcgc gccaaacgaa agcgcccgcg ccggagcgga gatctggttg aacgaactgc
     1141 tctggcgcga gttttacctg tctatcctgt accactttcc gcgcgtgctg gccgaatctt
     1201 ttgtgccggc gggcaagaac atccgctggc gtcaggcccc gcaggacctg gccgcctggc
     1261 aatccggacg cacgggctac ccggtggtgg atgccggcat gcgccagttg gcaagcctgg
     1321 gatggatgca caaccgcgcc cgtatgatca cagcctcttt tctggtcaaa cacctgctga
     1381 tcaactggca ggaaggcgag gcgtggttca tgcgccagct ggtggacggc gacccggctt
     1441 ccaacaacgg cggctggcaa tggacggccg gcacgggcac ggacgctgcg ccgtacttcc
     1501 gggtatttaa ccctattttg caagggctca gatttgatcc gctgggagta tatgtgcgcc
     1561 gttgggtgcc ggagctggcg ggcgtaccga cccgcaccat ccatacgccc tggcaaatgc
     1621 ccgcggaact gaagggggcg gctggctgcc ggctcggggt ggactatcca ctgccgattg
     1681 tggagcatcc tctggcgcgc gagcgcgtac tttcggtgta ccagcaggca ttccggcgct
     1741 gatatcccgg catagaaaaa ctcctgttgc aatttaatcc aattttcccc cgcctggctt
     1801 gagaatgtga taaaatcctt gctattcata gattgaatag tgaggatttg gtatgtcacc
     1861 ccgagcggcg ctccccctcc atctggaata caccctgctg ggtttgctca agcaaaaacc
     1921 ccagtacggc tacgacattc tgcaaaactg ggacaagaac ctgggtatca tctggaaggt
     1981 gaaaccgggc aggctgtatg ccgggttgaa caagctcgaa cagaacggcc tgatctccgc
     2041 gcagctggaa agcggtgaaa atgcgcctgc ccgcaaagtt ttcgcgctca cagctcaagg
     2101 tgaggcggtc ttctcgcgct ggctgagatc tccggtgcct tccccacggg aattccggca
     2161 ggaatggttt gccaagctcc ttttcttccc cgaactgagc gctgaagaac gcggagtgat
     2221 atacgaagag caaatcaaag ccgcgcggcg ctggctgaag gcgcaaagcg agcttaacct
     2281 gacgggttcg gatttcgaag cgcaagtgcg gcgattcagg cagatgcaaa ttcaaagtat
     2341 ccttgccttt ctgattgaca cacagcaaaa ccaaatttaa aggagtattt catgaaacgt
     2401 ctctgcacca tttttcttct gttcaccctg ctcttatcag cctgttctca agccgtccaa
     2461 acacaggcgc ctcagacagc cgtccccatc gtcatcaccg acgccctggg tcgggaagtg
     2521 aagctcgaaa agcttcccca gcgcattgcg gtgacgggca aagcctcgct gatgatcctg
     2581 gacgcgctct atatgttccc ggaagccaaa gagcgcgttg tcgcctttga ggacgctgtc
     2641 cagggtgacc ttaatttcat aaagctcctg gacccgcatt acgccgaaaa agcgtttctg
     2701 aagcgcgacg ccggcgtgga gcaattcgtc agcgccgagc ccgatttggt gctcctgaag
     2761 agctcgcagg ctgagaaata cggcgccccc atcgaagcgc tgaatattcc cgtcatttat
     2821 ctggattttg aaactccgga gcagtacgcg cgcgacctgg ctgtgctcgg tcagattttt
     2881 ggcaacgacg cccgcgcgca ggagcttatc cagctgtacc agggggcggt agacgaaatc
     2941 aatcaaaaaa tccccgcaac tgccgcgaaa ccaagcgtgc tcgtgcttca gtatgccgac
     3001 gcggacggcg cggtggcatt cagcgtgccg ccggcgggct ggatgcagac ccaaatcgtg
     3061 gaactggcag gcggcagccc ggtctggaaa gacgccaacc cgggcaaggg ttgggctcag
     3121 gttaccattg agcaagttgc cgtgtgggac ccggaagtga ttctggtgat atcttatgat
     3181 aaggacccaa gcgaagtcac tgccacgctg aagcaggacg cccagtggca gcagctgcgc
     3241 gcggtgaaag atgggaagct gttcggcttc cccaaggatg tctacagctg ggatcagccg
     3301 gacacccgtt ggctgctggg cttgaagtgg ctggctttcc gcctgcaccc cgctgagttt
     3361 ggtcaggtgg acgtcttgca gtccgcgcgc gaattttatc agtccgccta cggcatggat
     3421 gcggctgcat acgatgccaa cctcctgccg attacccagg gtgatatcga ttgaacgaag
     3481 atgcttcgct tctgaaagcc cgccccgacc gcgcccggaa gaaatgggtg tgggctgtgc
     3541 tggttgccgc cctcgtcctg ctgctggcgc tgtcagtctt tttggggcgc tacccccagg
     3601 tgggttttct gcgcctcagc caactgcggg aaaacctgct cgcgcagctg ctggtggtca
     3661 acgtgcgcct gccgcgcctg ctgaccgcct tgctgctggg catggttctg gcggcttcag
     3721 ggacggtctt tcagatgata tttgccaatc ctctggtcga gcccggcttt ctgggagtct
     3781 cccagggggc ggcctttggc gcagcgctcg cgattgtgtt cttttcagct tccgccgcgc
     3841 tggtacaggg gatggcggcg cttttcgcgt tcgcagggct gggcttctcc tacctgctgg
     3901 cgcggcgcat ccgcgtgggg agctggctgc tgcgcctggt tctggcgggc attgcggttt
     3961 cggcgctctt ttccgccggt ctgggcatcg tcaaatatct ggctgacccg ctgcgccaac
     4021 tgcctgagat taccttttgg atgctgggtg ggctggcgag cattacctgg accaaattcc
     4081 tggcggtcct gcctgcctgt ttcctgggtt tgacggtaat ttttgccttc cgctggcggc
     4141 tgaacctgct ggccttggat gagaccacgg cgttctcgct cggtgcggcg ccgcggcggg
     4201 agaggctcgt gatgatgatt gcggcggtcc tggccacttc agcgattatt tccgtggcgg
     4261 ggatggtcgg ctggattggt ttgattatcc cgcacatcgc ccggcgtctg gtcgggtcgg
     4321 atacgcgccg caacctgccg gctgccatcc tgctgggcgg ggcgtttggc gtaatttgcg
     4381 acgacctggc gcgcatgttg atggccgggg aaatcccgct gggcatcctg acctcgctgc
     4441 tgggggcggc gatatttatc ctcttgatgg cgcggggcct gggcaaggag gtgcgcagtt
     4501 gagcgttaac ttgctgagct ttgaccatgt tgccttcggg tactcccaag ccaggccgat
     4561 tttcagcgac ctgaattacc agcttcggcg cgggacgctt tctgttattc ttggtccgaa
     4621 cggcatcggg aaaacaaccc tgctttacat ggctttgggt tggctgcagc cttcagccgg
     4681 gcaggtgctg ctggaaaacc gcccgctgca agtgtattcc cgcgcggaac tgggccggcg
     4741 cgtctctttg gtgccgcaga ccgagcgcca gccctattcc tacacggtgt tggaatacgt
     4801 cctgttcggc cgcgcgccgc acctggcgct gtttggcgcg ccgggagaaa atgacgagtt
     4861 ggcggcgcgt caggcgctgg cccgcgcggg catcccggag cttgccggcg cgtatgtgga
     4921 ccagcttagc ggcggggagg ggcagttggt gctcatcgcg cgcgccctgg cgcaggaacc
     4981 gcgcgtgctt ttgctggacg agcccagcgc gcacttggac ctgggtaaca aattccggct
     5041 gatgcagacg ctttccgatt tgcgcaggga cggcacgacc atcctgatga ccacgcacga
     5101 cccggaaatt gccctggcag tcagtgacga gaccgtcctg gtggacggca gtggttcggt
     5161 gactgttggg accccaacgg atcttctgac cgcggataag ctttcacaac tgtaccacct
     5221 tcctgtgggc gcgctggaaa tccgctcctc gcaccccggg gtcactttac gccggttctg
     5281 aagtatggaa attgcataaa caaaaacagg cagacgaact tgaaatcctc tgcctgttta
     5341 tttttccggc tgcggggttc aggcgttcaa catttcttta aactcggccg cgctgatgcc
     5401 gcgtgtcagg tccgcgctga aatccatttg tgattccacc gcgttcttga ctggacctgc
     5461 cagggaagcg tcccccagca gaatagagcc gctcaggacc ccggcgcgga acaggaagct
     5521 ggcgtaactg ccgccgcttt cgtccacgag cagcaagtcc gagctttcgg aaggagtaat
     5581 ctgtccgatg ctgaacaggt cgatgccaag cactttcagg cgcgcggagg gcggatcgcc
     5641 agcgaatctg gcaggtttgc ccgccgcgga gaagcccgcg atggcgccct ggttcctggc
     5701 gggcacccac aggccgtaca tacggccctg atactcggta tcatcgccgg cggcgtaaat
     5761 ccgggggtcg gaagtgcgca tagtatcatc gaccagcacg ccgcggttga cggccagccc
     5821 ggctttgcgc gcgagttcca ggttggcgct tacacccgcg ctgataacta ccaggtcggc
     5881 gggcagcctg gcgccgtctt caagctccac gctttccacg tgttcggcgc caagcagcgc
     5941 tttggtcttt gcgccggtga taaccttgat gcccatggct tcaatttttc cctgtaagat
     6001 tcggctggcg gcctggtcca gctgccgcgg cagcagccag ggcagacttt ccagcacgct
     6061 cacctgcgct ccacgtcggg agatagcgcc ggctacctcc agaccgagca accccccgcc
     6121 aatacagacc acatttaccg gtcgggtgca gacagccagg atgctgttcg cgtcgtgcag
     6181 ggtgcgcagg gtttgcacgc ccgccagatt tgcccccggg aaaggcggca caaaggggga
     6241 cgcgccggtg gtcaggatga gcgtgtcgta atcgtagctg cgcccatccg cgcaaaaaac
     6301 ccgctgcgcg tgcgggtcaa tatccaccac cttgctgttc aggtgcagtt cgatgtggtt
     6361 ttccaggtac caggcccggg agtggaggtc cagcttgctg tcgtccagtt cgccggccag
     6421 gtagcgggtg aggttcatcc gaaaatacgg cagttccgtt tcatcggaga gcaaaactac
     6481 cttggcctcg gcttcggcct ggtggattgc gtcggcggcg ctgacgccgg ctatcccggc
     6541 accaataatc aggtatcgtt tcccagccgg gcggaattct tcgaagtggt tgcggtcggt
     6601 accgcactgc gggcattcat ccgggggttc tacgccgacg gaggagaatc cgcacacatt
     6661 acacacccat ttctttaccg gtaattggtt cattggtcgc ctctttgaga gcaatcgtaa
     6721 ataatttggc agtttgtgca atgagtgtag cataatgcca gcctgatttc aggtgcgtat
     6781 acaatcttta tccctgcggc tgtagaaggg gaaagcagtg tgtataatta gatccgaaag
     6841 atacccggag ggaattcttg tgagatcagc attgagcgcg ttttggttat tggccggctg
     6901 cctggcggtg attgtgctgc tcggactatt tgtcgcgcgt ttgcgtaaag cgcacgggat
     6961 aaagagcgcg ccgggttatc tgggcggcat catctgggtg attgccatga ttggctacgc
     7021 cgtcctgggc aggaacgaga tggcggaccc gcttattttc atcctggtaa gcagcgttat
     7081 tccgttggta tacctggtca acatcctcat catcctgctt tccaggcgct acacctggaa
     7141 agatccgcaa gacaagcgcg cggcaggccg ttttgccggc gtgattctgg cggtcttgct
     7201 ggtctggatc atcgggctca tccttgtgta aaaccgtttt caaaacccga aaacaacaac
     7261 caggcgcttg gggctataat tggggggctg taactaccac acgcaggagc tacctatgag
     7321 taacgaaacg acaacccgac caaaaacctc atttgcagat tggattctac ggctgctgaa
     7381 aggcgtcctg gtcggaattg gatttatcac gcccgggctt tcgggcggcg ctctggcggt
     7441 ggtgtttggc atttacgaac cgctgatgcg ctggcttggg aatctgcggg aaaagttcct
     7501 ccagaatctg atttactttg tgccagtggg gattgggggg attcttggtg tgctggcttt
     7561 ttccaaggtc gttgaatggg cgttcgggca ttatgcggcc caattcacct ggctcttcat
     7621 cggcttcatt gcaggcacct tcccctcgct catcaaaacc tccggaaaac aaggccgcaa
     7681 aagctggcat gtgggcttgc tggcagccgt cgcggtgggc acactcttcc tgatgcgctg
     7741 gctggagaca atccgcagcg tgcagatggt gcaaaacttc ggcagctggc tgctgagcgg
     7801 cgcgatgatt ggcctgggcg tggttgttcc gggcatgagc ccttcaaatt tcctgattta
     7861 cctcggcttg tacgaaccca tggccagcgg catcagccgg ctggactttg gggtcatcat
     7921 ccccctggtg ctgggcggcg cagcctgtgt gctgctcttt gccaggctgg tatcctggct
     7981 tttcaaaaag tattacgcgc tgatgtacca cataatcctg ggcatcgtcg tcggctcaac
     8041 cctggccatc atccccgcgg gcgtgcatgg gttgggtatg attgccgttt gcgtgctgct
     8101 ctttgcggct ggcgggctgg tttcttttct gctggccaaa ctggacgaga aatattcccg
     8161 ggacgctgtg gaataaggct tttgtgttcc aataattccg gaactgaccg cataaatgcg
     8221 taaattcggg ggagtaattg tgattgctgc cctaagaact gcttttatcc attaaaatag
     8281 aagtggtaaa gcagcgaggt ataaggatgc aaagagattt agagaatgcg gactgggccg
     8341 tcgggttgat gcaggggcgc caatccgtgc gcaattttaa ggaggagccg gttcctgagg
     8401 aaatcctcta tgaagtgctt gccgcgggca ttaacagtgc gactggtgga aacctgcagc
     8461 cttattccat catcgttgaa aaaaaccggc tcaggaacca gcagctggcg cagctttgcg
     8521 gcggtcaaag ctttatcgcg caggcgccgg tgaacctgat attcctgttg gactggcaca
     8581 agctggcggt ctacagccgc aaaaaggacg cgcccttcac ctgcaacaaa agcttccggc
     8641 atttcctcat tgccatagag gatatcgtat gcgccgcgca gagcatcgaa agctcggcat
     8701 ggctgtacgg cattggcggc tgcttcgtcg gcaccatcct ggagtgcatt cccgccgttg
     8761 cggaaatgta caagctgccc atgctgagcg cgccggtgct gctcctgagt ctgggctacc
     8821 ccaagacgct tgccgtgccg cgcaaaaagc tggacatgga tatggtcgtt ttccgcggca
     8881 cctacccggc gctcagcgcc gagaagcttt gcgaagccta cgaccgcaag tattccggca
     8941 tgcgcttctc gttacccgcc gacccgcaca agcggcaggc gctcttggac gagttccgcc
     9001 aggcgctgcg cacgacctac aacgagagca aatgcgacga aatcattcgc gcagcggaca
     9061 aagccggctc gatttccgag attcagcgcc gctttgggct gcactaccat gccgcggaaa
     9121 tgctgagccc cgatatcctc gaaggcctcg ccagccaggg aatctatcct tattactgcc
     9181 tgctggacca ggatccggaa ctataatcag cgcgtcattc tttgtttcct gatcaagatt
     9241 gaggagcgat tgcctttaat tcagcctgtg tttcttcgtt ggttgataac ttacaaaacc
     9301 atttccttaa cagcaaagcg gctgttgcat cagaaattat gccatgcgaa tcatcaatgc
     9361 tttggtcctg gaggctatcc atgagcgctg ttcttgaagt tcacaatctt gtgaaaaaat
     9421 acggtgagaa taccgcggta aacgatgttt ccttcgatat tcaggaaggt gaaattttca
     9481 gcctgttggg cccgaacgga gccggcaaaa ccaccacaat ttctattctt tccaccttgc
     9541 tctcgccaac cgctggcgac gcgaccatct ttggacattc cgtgaccagg gatccgatgg
     9601 aagtgaagcg cctgatcggg gtcgttccgc aggaaattgc gctgtatgac gacatgagtg
     9661 cgcttgagaa cctgaatttt tggggcgaaa tgtacggcct ctccggcggc gcgctgaaaa
     9721 cccgcgtgaa ggagctgctg gagcaaatcg ggttggctga ccgggccaaa gagcgcatca
     9781 agacctacag cggcgggatg aagcgccgcg tgaacattgc cgtggggttg atgcatcagc
     9841 cccgcctgct gtttatggac gaaccgaccg tcggaatcga cccgcagtcg cgccgggcaa
     9901 tccttgattc ggtcaagtcc cttaacgcag ctggcatgac cattctgtat accacgcact
     9961 acatggaaga agcgcaagag ctttcggacc gggtcgggat tatcgaccac ggcaaactga
    10021 ttgccctggg cacccagtct gagctgaccg ccaaagtcgg ggagatggac accctaatcc
    10081 tgcaccttgc tgaaaacgag gactccgggc acctggcgca ggccctgaaa acgctcagcg
    10141 gagttcttca ggcgaacccg agtgaccata cggtcgcgct ggtaacgccg gtcgccgaag
    10201 agctgctcgc gccggtggtt tcacgcgcca acgagctggg catcaaaatc caatccgtgg
    10261 atatgcgcga accgaacctg gaaacggtct ttttgcatct gaccgggcgg gctttaaggg
    10321 attgatgcga tgaacctgcg caaacttctc cttatcgcgc ttaaagacct gcgcttgatc
    10381 ttccgcgatc cgacaggtct ggtaattatg ctgctggcgc ctttcgcgct aacgctgggt
    10441 atgggggcgc tctccggccg cttttccggc tccacgcagc tcggaatcag ccagatctct
    10501 gtggcgattg tgaatcagga cagtggggaa ttggggcagg ctctggagga cgtgtttact
    10561 tccagggaat tgaacgaact catcgccgcg gacatcctga ccgacccggc tgcggcgcgc
    10621 gccctggtgg atgaggacca gattgcggcg gccattatcg ttccggaggg atttaccgcg
    10681 agcattttta gcccggaaag cgggggagac gtgcctgtgg tggagttcta tgaaaacccg
    10741 acccgaccga cgtccacagg catcgcgcgc tccattctgg agagcttttt gaatcaggtg
    10801 gaaatcgggc gggtttcggg cacgctgatt gtcagtcagc tgctggcttc cggcgcaatc
    10861 cagccgcagc aagctgcagc gcttggcgcg gaggtggggc gggaagtagt cgggagcacg
    10921 gaagacagcc cttccatcaa gctcaacgcc aacaccgtgg aaggcagtgg agcgaaattt
    10981 gaccttctgg cctatatcgc gccgggcatg gcaatgttct ttctgatgta taccgtgacc
    11041 atgggggggc gctcgctttt ggtagaaagc agaaccggga cgctggcgcg aatgctcatc
    11101 tcgccgacca atccgggctt tgtgctgggc ggcaaaggtt ttggcatctt cctcacctcg
    11161 gcggcgcagc tgcttatctt aatcggcgga acctcgctgc tgttccgcct ttcctggggg
    11221 gatggaacgg ctgtcgcgct gctgattttg gcggcggcat tcggcgcttc gggctggggc
    11281 atcctgatgg cagccgtgct gaaaacgccc gggcaggttg ccattaccgg ttccgctatc
    11341 gcgcttcttt tcggcattat aggcggcagc ttcttcgacc tgcacatgct gccggagtgg
    11401 gtgcgcacca tagcgaaggt tacccccaac agctgggcga atgagggctt tcgcattctt
    11461 tccatgggcg gtaagttagc cgcgatacaa ccccatctca ccgcgctgat gagcatgggc
    11521 gcgctgcttt acgcgctcgg gacaatcctg atcagccggc gcggattggt gcgaaagtga
    11581 ggcgcgcgat gaaaaaactg tttgccatca tgaagaaaga tacgatcata cgcttcacaa
    11641 gcccaatggt atggatgttc tttattatcc tgccggtagt gttcattttc gtcatcagcg
    11701 gcggtaccac ccaaaatagc gacccgcgga ttgtgctgac tgtcgttgac caggctggta
    11761 gcacgctttc gggcgcgctc gtggaggagc tggggcattc cagcgcggtg aagccggtgc
    11821 tgaccgagta cgaccaggct ttgaaggact ttgagcagct caaagtctcg gcgatgctga
    11881 ttatccccgc ggatttctcg gccggggcgc tgacggcggg taatgcctcg ctggaactgc
    11941 gcaaacagcc aaatacgctg aacggcatgg cggtggagca ggctgtacaa ttggcagcca
    12001 gcaggatagc cagcctggcg gatgcggcgc gggtgagcac agaacgcgcg gcagaatttc
    12061 agcctttcgc cgatgacgcg gcgcgccagg cgttctatga cagcgcattc cagcaggctc
    12121 agatactgct tgccgaggct ccggaccggc tgcaaagcgt ggtcggcagc acgaccgacc
    12181 cgatcgaata cgatgccaaa gccaactcca ccgccgggca gatgattacc tttgtcttca
    12241 ttccgcttgt cagcctatcg gtgatgttcg cgtacgaacg cgccaacggg acgctgcggc
    12301 gtttgttggt tacgccgacc agcaaggcaa cttttttggg cggcacgatc ctcgggcagg
    12361 ggctgaccgc cctggtgcaa atgaccattc tgattgtgtt cggcattctg gtgatgaaga
    12421 tgaagtgggg gcagtcgccg gccggcctgg cgatggtgat ggtgtctttc acgctggcgg
    12481 cttcggctct gggcacgatg ctgggcacgt ttgtgaagag tgaggggcag gcgaacgggc
    12541 tttcgttgat gattgggatg ctgatggcga tgctgggcgg ctgctggtat ccgatcgagc
    12601 tcttccccgc ggcgctgcgc aacgctgcgc aggctctgcc gacctactgg gcaatgcagg
    12661 gctttttgga tatcgcggtg cgcgggcagg gaccggccgg ggtgctgcag gagtgcgcgg
    12721 tgctgctggg cttcgcgctg gtgttttttg cggttggcat cacgcgcttc aagtacgaat
    12781 aggggctctg gctttcatgt catcctgagc tagcgatgtt gtcatcctga actaaaaata
    12841 ctgtcatcct gaactcagtg aaggatctgc tttttgtaga ggtgagattc ttcgcgtccg
    12901 ctccgaatga caaaatgtca ttctccctgc cttccgtcca ccatcatgcg tactctcgtt
    12961 gggtttcgct cctgcgtcgc tgcacccaac ctacaggctg cgcccaacat gcatgcgaac
    13021 agggtgttga tgttgggttt cgctcctgcg gcgctgtacc caacctacag gctgcatcca
    13081 acccaaatgc gaacagggtg ttgctgttgg gtttcgctcc tgcgtcgctg cacccaacct
    13141 acctgctgca tccaacccaa atgcgaacag ggtgttgctg ttgggtttcg ctcctgcgtn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gctgcaccca acctacaggc
    13321 tgcgcccaac atgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcctgc
    13441 gtcgctgcac ccaacctaca ggctgcgccc aacatgcatg cgaacagggc gttgctgttg
    13501 ggtttcgctc ctgcggcgct gcacccaacc tacaggctgc acccaaccta catgcgaaca
    13561 gggtgttgct gttgggtttc gctcctgtgt cgctgcaccc aacctacagg ctgcgcccga
    13621 cctgcatgcg aacagggcgt tgctgttggg tttcgctcct gcggcgctgc acccaaccta
    13681 caggctgcac ccaacctaca ggctgaactc acgcaaaaga ggctccccgc agggagcctc
    13741 ttttacatcc ataataatct gtcagtttag cgcaccgtaa tctgggtgta ggctggcgtg
    13801 atcgccccgt ccaggtccgc caccaaaaag cccaccaggt actgcccgtc gggggcgtaa
    13861 acctgttccc aggagaatgc gctcgtgcca aaggtgagcg tatcgccttc ggtgacggtc
    13921 tggccgctca ccttgccgct gctgtccagg ttcaaccagc ggtaggcgat ggtgaacgta
    13981 tccccggcgg ccggcgtaat ctgcgaaggc tgttgggcgg cgtccccccc tttgaagccg
    14041 aacacctgga agagctgtcc gtccttgaaa tacagctcgg cagtgcgctg ctcgccggta
    14101 tcagcgaatg tgtaggttcc gcgcaccgca taaaccgcct gttccgcgct ggcgccgtaa
    14161 gaaaccgggt tgaacatcgc cagggcggac tggctgccgt cggtgatgga gaagagcagc
    14221 ggctcaaagt cgaagttcat gcggaaagcg ccgccttccg gccagaccgg gtaatacacg
    14281 ccgttcagcg agtcctgttc cgggctctcc aggtagtcgg tatcggcaat gaagatgctg
    14341 ttggaagcct catcgtacaa accggtgaac aggtagatat agccgatgtt cgtgccggtg
    14401 atatccgcgc tgagcttgat ggagccgccg ggcgtaacgc tgctggcgga tgcctggatg
    14461 ggcgagacgc tgatttgccc gccgcccggc gcgcgtgtga cggctgagga ggggggcgta
    14521 accgcctcgg ccgcgctttc cttgaaggag cggttgctgt agtggaacac caggaagtca
    14581 tcccagaggg aagcccttga gaagcggtcg gccagcatgg tatacgagcg catgccagtg
    14641 gctgcgttgc tgtagagctg ggaattcggg aagtaaatgg caataccggt agcgccgggc
    14701 ttgttggggc cgtgtttttc ggccaccagc acagcgttca gcgcgttgat gacgtcattc
    14761 gcggattggc ggatggcagc atctccgccg gactttttgt agagcagctg gatgaaatgc
    14821 cccaggtcga tgtacgaagg aggcacctgg ctgccaaaga tgctggtgaa ggactgcgcg
    14881 tagctgcgcg cgctggcgac cacctgctgg ttgagggact gcatgtcgta aaccaggccg
    14941 ttgaaagcct ggttcagcgc gggtaaattg tccaggttca ccgcggtgag ggtcacgttc
    15001 tgttcaagct ggctggcaag ctgttcggcg ctcatgcgat agctcgagaa aaagccgccc
    15061 gagctggagt tctgcgccaa aaagtcattg cgcgcctggt cgtccgtgac gcgctcatcc
    15121 tgctggatat aggtttcgac gatgttggtc gccaggtcgg cggtatcgat gcccgggttg
    15181 tcaatcagca ggctgatgaa agcggaatac gcccaaccca agccgggctc cgtctcttcg
    15241 gatgccacgg cgtaatgcgc gtagggctgc agggcggtgt aaacttccag ttggctcatc
    15301 aggcaagcat ccatgccaat caaatcgaac ttttcgatgc cggtgttcgc ctggatttgg
    15361 gccagcgcct ggtcaagctc gttcaggtag atggagtcct gctggagggc attgataatc
    15421 ggcgcgctgc cgccgtccgg ggtggcaggc gcggggtcgc tccagccgcc cggccaaccc
    15481 atgccgtggt cggacataat cagcatatag cggtccgccg gatagttctg gacagtccag
    15541 gtgacaaaat ccaccaggct gttaccgtca gccatgtcca cttcgcccag gtccatcaac
    15601 atatctgagc cgatagtgta caggtcgtcg tcgtacaaca ccagatagcg cctggctgaa
    15661 aaccagttgt ctgttcctga gtagccgcct ctgaagcggt ccatctgcgt gatgatgcgc
    15721 acgttgtccg tggagccgat gcgttccatc tcgttcatgt cgataaaaac gtcctcttcc
    15781 agggcctggt cgtccgcgtc ctggtagacc atcacagtcc aggtgtcccc ggaggcgctg
    15841 gtcgggcgcg gggtgcgcgt gacgggcagg gcggtcggct gctgaacgac cgtctggggt
    15901 tcctcggtgg ggtaatcata gccgggctcg gcgacctggc tgccgtcgcc gcggaaaaag
    15961 aggaaataaa taatcgcggc aatgatgaat aagccgattg agccgcagcc cgtttttccg
    16021 ccggtggtgc gtaagacatt gccgccgggg atgccgctgc tgccgccgga ggaagcagag
    16081 ccctcgccgg agtaagcgcc gccgctggaa ggccgtttgg gggcttgcgc ctggctggta
    16141 ggcttcgcgc ctttgcgccg gcgctgaaca ttgacctcag ggagattatc agacattttc
    16201 accgtccatt catttgtctc ggttgtaatt tgattatatt gcagaatgta attttcgcgt
    16261 tttccggggg tccggcgccg gcaggctgtg cgaagttttc cgtgcgggaa cagggttgat
    16321 ataatctttt gcagagagcc tgataacaga tgcactgagt gcgaaaagga gagactgtga
    16381 gtgtaaaacg gattgaagaa gtgcctgctg agcttgtccc ggatgctttc ggcgtgagca
    16441 agcaggtgtt gatttccgcg ggggaagcgc ccaattttgc catgcgctgc tttaccatcc
    16501 agcccggcgg cagcatgccg ctgcatacca acctggtgga gcacgaacag tacgtgcttg
    16561 gcggaaacgc gaaagttgtc atcggagagg aaagctgcct ggtggggccg ggcagcgtgg
    16621 tgttcatccc ggctggcgcg gcacacgctt atgtcaacac cggcagcgag cctttccgtt
    16681 tcctgtgcct catccccaac cgcgaagaca aaaccagctt cgtttccagc tgatactgct
    16741 tgacagcgag ccctgcgcgt gtaaaataat cagttatttt acggccaata aacaggcgca
    16801 aggcctgctc aaatagaaag ctcaacataa gcgatgattt cacaactgcc ccctctcgtc
    16861 aatgtgcttc tgctcagcct gattgccagc ctcggcacag gccttggcgg attaatcgca
    16921 gtcattcgtc gacccggcaa acgctcttac ggatttctga tgggcatcac cgccggcgtg
    16981 atgatctgcc tctccttcct ggaactggtg aatgaagcct gggatctggg cggtccgatt
    17041 accgcaaccg ttggttttgg cttgggggcg tcctttatgt ttctgctgga ttacctggcc
    17101 ccgcatatcc gctttggcga ggtggagtac cacggtgcgc acgccacgga ggctgaggcg
    17161 aacgactgcg aggacttccg gccccgcagg cgttttcgct ttgccaggct gcgcgcgcag
    17221 ggcgcccaaa agaagccggt ggaccaaaag ctgatcagtt ccggcatcct gttggcgatt
    17281 ggcatcacgc tgcataacct gcccgaagga atcgcggtgg gcgcgggcta cttgcacagt
    17341 ccgcagtttg gcctgttcgt ggcgttggcc atcttgctgc acaacatccc ggaagggctg
    17401 gctaccgcgc tgccgctgtg caaaggcggc gtgcgccggc gggacgcgct gcgcgtggcg
    17461 ttcttatccg gtttcgcgga gccggtgggg gcgctgctgg ctggtctgtt cctggtgaac
    17521 tttacgaatt tcatccccgg cgcgttggct tttgccggcg gtgtgatggt gtttatcacc
    17581 ctggacgaac tcatcccttc cgcgcgcgaa tatgggcatg tgcactggac atctctgggc
    17641 attatcctcg ggtcgctgtt cgtgttcgtg ctgtctgccc tgctgggcgt ctgataatcc
    17701 cctttgtaag cttacctgaa caataacggc agatgcgtga ttgccgcgct gcnnnnnnnn
    17761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntccccttt gtaagcttac ctgaacaata
    17881 acggcagatg cgtgattgcc gcgctgctca ggcttaggcg gtactgcgcc gcgctgcctg
    17941 ccgggttgag gaaagcttca tccatttcgg gtttaaagag ctcgaaggtc agcgaattgc
    18001 ctggcaggat gcggatgaag gcttcccaca aggcggattt tacgtcgggc tctgcgggct
    18061 gtggaggctg ccaggcgctg gctgccagca gaaagagcgc cagaatgata atgctgaagc
    18121 gataccggct tatagaccct acgattttag aaatggaacc aggacatctc cagtagattg
    18181 gattgtcggt tgaattccac caatttgtgc aagcccggta cttgcgttgg caggccgggg
    18241 cgctattccg tgctgcggat ggaatccagc tggtaatacg atgctacgca gccgttcacc
    18301 cactggctgg ggtcggggga gagttccatc aagcctgcca ggctgtccac tcccgggacg
    18361 agcaggttgc gaaggccggc ctctttttgc tgcaggatga ggacctcgcg ttcgtcccag
    18421 gcggcggcgt gccggcggaa ttcagcaaag tgcgcgggca attgttggag cgcgaaaaac
    18481 agcgagacca acgcgccaag gttcaacaga atatgcagca tgcgcgagga ctgctcagaa
    18541 aggagcgcgc gcagccaagc gccagagagg gcacccgccc ccaacagcag cgcgctgatg
    18601 agaaaagccg caacgctttg ggagcgcgcc tccgggtagg cagattgcgc cagggcgccg
    18661 ggcgcgcaaa aggcagctaa aaggaggaag gctgcgagcg cggccagcag cagtgggggg
    18721 ataagtttct tgggagaggg cagcctgggc tcgcgcacgg aaaggccgaa gagtaaaccg
    18781 agcagcagcg cggtcaaccg ggagggcagg aaatccagca ggctgtgata gaccagcgcg
    18841 gcggtgtggc gaagcgtgag atagataaga cggcccagcc ctggcatcgg cggcatttgc
    18901 gccaggcgca ggtgcgcgcc gggctgcagc gcaagcacaa gcaatccaag caggctgccg
    18961 accagcgcgc taacccaaag cccgcgctca tgggcgccaa ggcgcttgcg ccagagcagc
    19021 gcgcaaaaag ccaggcccag cgcggtgaac tgcacagccg cgtttacggt ggaaaagccc
    19081 gctgccagga aagcgcccag agcgaataga acctgtaggt accaggcggg tttgtttttt
    19141 cgcgggcgca atagccccgc caggagcaga ttcagcgcga tgacaggcag ggtataggca
    19201 aggctgccag cgcgccaata cacggactgg tacaggttag gcagcgcgga aagcgttatt
    19261 tctatgagcg ccagcgagca gataagcagg ttcaggcgcg gctgcccggc gttcaatcga
    19321 aggaggaact gcctgagcag caggtaaacg ctggccgcaa gacccaaaat caggagaatg
    19381 ggaagcacgc gcagccccca cagtccgaat ctgtcgagaa tgccggtaaa aaacatggtc
    19441 gcgtagcggt tggtccaact ggtaaaagcg ttagtttgcg cctgccagaa gccgagtgag
    19501 cggaattggg ccgaggagca atagtcgtct gcctggggtc gggcaaaggc gctcaacgcc
    19561 agatagggcg cgaggcacag cgccagcagc agcgcccagg cggcgctcca gctgtgcttg
    19621 ggcgcatttc gcccgggatt ttctttgtgc gtcgcgttcg tcatcttttg ccgccctgcc
    19681 tgttggttcc tttggtctga ttatagtgct tttccggttt tttttgagcg cgggaaggca
    19741 gagacggact ttggaactat acgagaattg atcgaaaatg tccaaccccg ggtgatttcg
    19801 ttgccagccg atatgcgtct ccgagaatca aatccgggcg aagcaccctg aaggcgtgca
    19861 ggtggggata tggaaaattg ctttgggatg cttagcccgt ccgtgtatca caaagcgtaa
    19921 agcgcgtcgg cagattgcct gtgcgccaag cagattcagc agccagaaaa tgagggacag
    19981 cgccgcgcgg attttgggta ggggcgaagc accctgtaag cgtgcaggtg gggatatgga
    20041 aaattgcttt gggatgctta gcccgtccgt gtatcacaaa gcgtaaagcg cgtcggcaga
    20101 ttgcctgtgc gccaagcaga ttcagcagcc agaaaaagag ggacatcgcc gcgcggattt
    20161 tgggtagggg cgaagcaccc cgtaggcgtt ccggtggtga gatggtaatt ttctttggga
    20221 tgtttagccc gtacgtccgt aacacagaat catcgaatcc cgccggccaa agaactgctt
    20281 tccccctgat gcccggcagc ccatccaaat gcccgcttca ggatataatc aaagcagaga
    20341 aattcaggag gagcatactc acccgagcct atgaatttca gcatgcaagc cacgtacgca
    20401 ctgcccgccg cggcaaacac cttcgccttg aaagcaaggc gcgtttatcc tatcctcacc
    20461 ccccattcac tatcaaagga gatgtaatga ccgaacagcg taagaaattg acccttcagg
    20521 gattgttcaa aaaagcggct gaaggaaaac cgattacctg gctgacctgc tacgattatc
    20581 cgacggccta ccttcaggag caggctggcg tggatatgat cctggtgggc gacagcctgg
    20641 gaatgaccat gttgggttac gacagcaccc taccggtgac gatggacgat atgatccgcc
    20701 acagcgcggc ggtgcggcga ggcgcgccga acacctggtt ggtcggagat atgccgtaca
    20761 tgagctacca gccctcaaac gagactgcgg tgcgcagcgc cgggcgcttt atggccgaag
    20821 ccggctgcga ttccatcaaa ttggagggcg gtgcggcagt gtgcgaacgc atccgcgcga
    20881 ttgctgatgc gggcatcccc gtcatcgggc acctgggcct caccccgcaa agcgtcagct
    20941 cgctgggcgg cttccggctg cagggcaaga gcgccgcaac tgccaaaaag attctggacg
    21001 atgccaaagc gctggaagaa gccggggcgt tcatgatatt gctggaactg gtaccagacc
    21061 ggttgtgcaa actggtaaca gagcgggcga agaactgctt gattatgtcg ctcggctcgg
    21121 gtctggacgc gcacgggcag ctcctgatat accatgatgc cttcgcgctc tatcccaagt
    21181 tcaagccgcg catggcaaaa gtcttcgctg atgctggctc cgttattcgt cagggtttgg
    21241 aagcctatgt gaaagaggtc tcggaaaaga ccttccccgc gccggaaaac tggtttggta
    21301 tgtccgacga cgagtataac gaactgctga gtatgcttta ggagaaccga aatgagcgac
    21361 ctgcgcgaaa cgctgaaaat ccccgccggc aatctggaag cagtcaatag cgttctgttg
    21421 gaccctgaca gccgcgttgt caacgccttt ttggatgtgg tggcgcgtta tggcacaccc
    21481 gaagaaatca accgcaaaca ccgtgaatcc cgccggatgg aagtcctgtt cgagaaaatc
    21541 cgtcaaaaag cgccgcaata cctggaggat ttgcactggc tgattgacca gcgcagccgc
    21601 ggctctttcg tccgcctgga agattaccgt aaaggcattc taggggagga ttttgacccg
    21661 aaatgcatca acgagagctg cgcggtcact ttggaagtgt cggcgctgca gtacttcccc
    21721 tgggtgcgcc cgatgttgga gcaagctatc gccaaccgga ctctggttcc cggacgcttc
    21781 attgctgtac ggaaaatgaa agaatctgaa atggacggcg acctgccggc catcgtggcc
    21841 gcgctggata tcattggcgc ctcgttcgtc gaaacgttgg ataccaaagg cactgacggt
    21901 tccaacccgc acctgggagg cccggccacc ctcaccggtt acttcggcgg catcggggag
    21961 ccgaacgggc acgctttgcg ctggctggat gagttcctgt actactacac caattacggc
    22021 gtgcagcacg tcctgaactt caacgccggc acgattttgc ttgggtttat gctttacagg
    22081 ctgggcgtgg atattcagtt taagatttcg gtctattttg gcgcggataa cccctattcc
    22141 gcgctgtgga tcatgaccat ggccaagctc ttcagccgcc ccgacggtac ttctccattg
    22201 attggcttta actggtccaa ctccatcaac aatgaaacgc tggagttgag ctctgaattc
    22261 cgcaaggcgc tcggcttcga gcgcgtcatc cgctttgagc accacattac ggaaacccag
    22321 aaaagtatcg tcatccaacc ctacaaccgc cggcaggaac tgctggaagt ggcggagcgg
    22381 gtgcccaaca tcgctgccaa acacgaaggc ggcgacccgg aaatagacgc cgcgcgtgag
    22441 cacccctcgg atatcctgga ttatttccgc gataaagctg aggtcattgc cagcggggac
    22501 tgggagcatc tgcaagtgaa ctttttggat aaggtggcgg cttgcaatgc cactgcgcgg
    22561 gcgctgaccg aacgcaagct gggctttgtg gccgcgcgca acctgcatat ttaaaagact
    22621 ggcgcggctt ttgcagggct aatcaaggat acggacgcag cagggctgac cagctgcgtc
    22681 catttggtgc atgtgcatag tgatttatgg tgagcgcgca gcgaaccggc gcacttcccc
    22741 gcgcgaaggc tccgcgcggc ttactttttc ttgtctttcc agtagttatc cgcttccgtt
    22801 tccaacctgt ccaggcgggt gtagtaatcc gggaattcgg tcaggtgcgc cagggcaatc
    22861 ttggctgtca tcagcgggtc gtcactggtg acattggtga gttggttgcg cgtgccgtgt
    22921 tccagctcca cgttgagccc catggtgaac tgctctacat taaagtgcgt ccagtccacg
    22981 cccaactgct ctgccagagc tttggcttct tcctgtgtaa attgtttcct cggtgacatt
    23041 gcttctgctc cttcacgata tggttgccag atattgtagt cctacccatg cgttttccac
    23101 agaggcattt tgggcaaagg gccggcttac gcgctaatgt agagaatatc gcttgtgccg
    23161 cgttcgccgt ttctgccgct gggctggttg actaccctgc ccataaagac catagtggat
    23221 gagccgccgc cgtccaaatt gtaggctgcg gtgcagccca gctcctggaa caggtctgcc
    23281 agttcggtca gggtcatccc gcggctgtaa ccgctgctgc gtccgtccac caccacaaag
    23341 acgtagtggt tcggcgctat gatcccaagc gcggtgcgcg gctgggcgcc ctggatggag
    23401 tggttgccaa aattgtggtc cacttccacg ctttccaacg cggcaacgac ttcgccctca
    23461 tccagaagcg ccggcccaaa ggaaagtgta ttgataaccc cgctgtccaa gagggcctgc
    23521 gcgtttgtag tggtttcgtc gtacacctgc atatttccgt ccgcatatag cgccagacca
    23581 acccgcgccg gcacatcccg ataaatcacc ccgttgcgga tgagaatacc gtcctcgcgg
    23641 aagccgtagt agtctccgtt gattgcgaaa acagcgtcgt ttgcggcggc aatttctgag
    23701 gtggcagcga tgatgttcgt gccgaacttg ttctcggcaa aggcgctgag gatgttcgcg
    23761 ccgtccttaa gcaccacatc ggccacgaag tatgtgattg tatccgcgcc tgaacccagt
    23821 gtagttttgg taatggtgat ttcgctggtc tggctgtgat aactccaatc atcccagacc
    23881 ggttctgagg tctgggtgga gctttccggc gcgctgctgg ctgcttccgg gagcgcttgt
    23941 gtgctcacag ccgcgctggc gtacgcgttc acgtccccaa tttccacgtg ccggatgagg
    24001 tatcggtcgg ctgccgcgaa cgcggccgct gccaggagga gggcgaaggc cgcgggaatt
    24061 agttttttga tttttggttt catgtgcatg ttcctttctg tctgccagcc atgcggccgg
    24121 caaagcgatt ttcagtctac cttttagcca gaaagcgtgg tataaatgcg gtttattgcc
    24181 gccgcccggc cttttttttg ctgcttatac gggttctata cgcgcgggag caattgaatc
    24241 actatactcc aaaaagaggc aaaaaggcgg caaaggttaa acatgatcgg attattgggt
    24301 tcacactttc tcaaaggctt ttggatgtat ctgttgtttg cgctttccgc gctggtggag
    24361 ggacccctga caatcctcac cggcggcgcg ggcatcgcgc ggggcttctt gcagccgctt
    24421 ccgatctact ttgccgtagt tgcaggtaat ctgcttggcg atatcggctg gtacagcctg
    24481 ggcaggttct ccaaacctgg gtgggtgtac caaatcgcgc ctaaattagg catcagcgca
    24541 gagaaagtca gcggactgca ggaaattgtg cgcaagcaca ccagcaagct gattttcgcg
    24601 gcgaaattca gcgcgggact ccctattcca accttgattg ccgttgggct cagccgcgtg
    24661 ccgaagcgtc agtgggcggc tgcctggatt tccggggaac tgctcaagtc agccatcatc
    24721 atgctggcgg gctacctgtt tagcgccgct atccagactg cctctcgtac aatgcaagta
    24781 gtcctctggg gtgtcacggc tttgcttgct ttggcgggta tcttctggtt tcggcgccgc
    24841 aaacataccc gggcgtaggc gcggcgcggg tcttgaaggg aagaaaatga aagaggaaca
    24901 aacgctccca aatgtattgg taatcatccc ggcctacaat gaagcagagc ggctgggccc
    24961 ggtggcggag gcggcgggtt ccctgcttcc agtgctggtg gtggatgacg gttcgggcga
    25021 tgagaccgcg gaaatcggca ggcaggccgg cgcgcaggta ttggagcaga cgccaaacca
    25081 gggcaaaggc gcagcgctgc gcgcgggctt tcgctgcgcg ttggcacgag ggtatgaggc
    25141 agttattact ctggacgcgg acggtcagca cgacccggcc gaaatcccgg cgtttttaca
    25201 ggttttccag caaagcggcg cggacctcgt catcggccgg cgcgattttt cccgcatgcc
    25261 cttcatccgc cggctcagca acacggtggg tacccgcctg ttctccaggg cgcttgggca
    25321 gactgtgccg gataaccaga gcggttaccg tcttatcagc cgccggctga tggaagccag
    25381 cctgaattgt gcagagcggg gttttgcttt tgaagtggaa atgattgtgg aatgcgtccg
    25441 gaatcacttc caacttgcct gggtccctat ccggacgatt tatgccgggg agggcagcca
    25501 tatcagcccg ctcaaacacg ccgcggagtt cgtgcgcgtc tcctatcgga ctcgccggag
    25561 cttacgcagg gcggattaag cccggcgccg cgcgttggca gggggagtaa gacggatggg
    25621 ccttctcgca atccgggcgc ggggacgatt gcttacccgc gaatcctttg ggatgattca
    25681 ataaagtttt gggccgcgat ggacaggtag cgttccttct tccaggctaa cccaacatcg
    25741 agggataagg gctccgccag tgggaggacg accaggtcgg gacgttgggg gacgatgtcc
    25801 tggatgaaaa aggtaatccc cattccttgt tctacaagct ccaggatggt cccaatctga
    25861 ctggatgaaa acaatatgcg cggtgagaaa tggaaacggg cgcattccgc cagaatgcgc
    25921 tgccgaataa aggtgtcttc cttgaacaga ataaactcga attcctgcaa ctggtcaaag
    25981 ggcacgcgct cgaggcggct cagaggattc gtgcgggaca gacatacata aatctgcccg
    26041 gacttcaccg gcagcacttc cagagaggat ggaaggtcag aggtaatcat cagagcaata
    26101 tccaattcgc tgttttcgag ctgctcgcgg attagcagtg agccttcttc cacgatgctg
    26161 accttcacat cggggtgcaa tttttggtaa gcgctcaaga agcgggggaa catcagcgct
    26221 cccataatcg gagtaatgcc aattctcagt gcgccgccca aagggcgctg gatatcactg
    26281 atttccacta cggcgtcatg caaatcccca aggacgtgct ccacgcgttt tagaaatatc
    26341 tgtccctcgc gggtgagggt tcctttctta tggctgcggt ccaccaggct gatacccaac
    26401 tcctcctcaa gttttttaat tgcgaccgag atggaaggtt gggagacccg taattgttcc
    26461 gcggcccggg tcatgctttc caatcgacca aaagcctgaa aatactccag ctggcgcagt
    26521 tccattggta ctctcctggt ttataggttt tgtttatgat aacataagca aaacattctt
    26581 gttatatata gtttctcatg atatagtaat tttctcagcc cttttaagaa ttctttgcgc
    26641 tgtacgcggc agcgagcttt ttatccgcag aaaggttctc catgaaagaa atcacactca
    26701 cgatcaacgg cgagaaacat tccttatcgg tccccccgga cctggtactt attgaccttt
    26761 tgcgtcggga tttgcgcctt acgggcacaa agcaatcctg cgaccgtaag ggacagtgcg
    26821 gagcgtgtac tgtcattgta aacggcaagg ctgtccgctc ctgcctgacc agggtcgcga
    26881 acctggatgg ggcagaggtg attacggtgg aggggctggg caccccggcg aacccgcaca
    26941 tgatacagga ggcctttgcg ctggctgggg ctattcagtg cggcttctgc acgcccggct
    27001 ttattatgtc cgccaagggc ttgctggaca ggaaccctga cccgagtgtt gaggaaatca
    27061 agaaggcttt tacgcataat ctgtgccgct gcaccggata caaaaaaatc atcgaggcag
    27121 ttcagttggc tggaaggttt atgaggggcg aaaccacgcc ggaggctgaa cgcgccagga
    27181 tcggccgcga gactgtcggc gtatcccacg tacggccgtc cgcgctcctg aaagcctgcg
    27241 gtgtggctaa gtttaatgat gatattccgc tgccggaaga cgctctggaa atcgccgtcg
    27301 tgcgcagcac ggaatatcac gcgaacatcc tgtcgattga cacgagcgaa gcggaaaaaa
    27361 tgcccggcgt ggttggcttc ctgcgggcat cagatattaa ggggactaac cgtttacgcc
    27421 aggtgcagcc cgatcaaccg gtgctgtgcg aagaccgcgt gcgtctgttg ggcgacccca
    27481 tcatcgcggt ggcagccaac acgcgcgaac aggcgcgggc ggctgcggca gcggtaaaag
    27541 ttcaatacga acccttaccg gttcaaatga cgacgcggga atcgctggcg cccggcgcgt
    27601 ttgtcattca tgcccacgcg cccgataata tttgctgtac ccagccactg atcaaaggcg
    27661 acgcagatgc tgccctggcg gaagccgcgt acatttacga aggcgagtac tccacccaaa
    27721 cgaatcatca ggcgccgttg gaaccggaag tctcctccgc ttattttgaa ggcgaaggtg
    27781 acgcgcggga actggtggtg gttggacgca gcattaacat tcatttccac ttgaatcagc
    27841 ttaaggaagc gctggggcac accaagatgc gctaccagga agcctattcc ggcggtcaat
    27901 tcggcatcaa ggtctggatt attaccgagg ctatcgccgg cgctgccgcc ctgcatttca
    27961 atcgccctgt tcgctacgcg ccgaccatgg cagagtccat gctgattacc accaagcggc
    28021 acgcgtaccc cagcatcaag attaaactca ccgcggacaa ggagggacac ttcaccgctc
    28081 tgttctgcga ctttaccatg gacaaaggcg cttacaccgt taacggcccc gtcattctcg
    28141 ggcgcgcaat catgatgctg accagttcct acaatttccc caacgtgaaa gcttttggca
    28201 gaaacgtgta taccaataac ggcgccggcg gttctgcgcg cggagccggc ccgccccagg
    28261 ttgctttcgc gctggaatcc gccgtggaca tgctggcgga gaagattggc attgaccccc
    28321 tggaattccg ccggatgaat tctctgctcc caggacagac caagggcgtt ggcatggtgg
    28381 cggacgaatg gtgcttcccg gaagtctgcg actctatcga ggatgcctac agacgcgcca
    28441 ggcagaaagc cgaaagcttt aaccagacca gcccggtaat aaaacgcggc gtgggtattg
    28501 ccgcgcactc gtttgggatt ggcggcgcag gggattccgc caagatggct attgaattga
    28561 acccggatga cagcgtcacc atttatgccg ccgttgccga cccgggcgag gggaatgacg
    28621 ccatgcttac ccaaatcgcg gcgcatcagc tggggctccc actcgagaag atacacctgt
    28681 acacgcgaga tactgacaag actgtattga tgggaccagc cgcgggcagc cgcatgactt
    28741 ttatggccgg gaatacgctt ttgaatgcaa ttggcaagct gcaggctgcc atggcggaag
    28801 ttggcgccgc cgattacgct gacctggtgg ctgccggaaa accaacccga tatgaaggtt
    28861 ttcataagaa cgatgtgccc gcgggactgg ataagcacac gggtcagggc tacgcccagg
    28921 tttcagagtg ccataatatc cagatggcgg aagtggaagt gaacactgaa accggcgcgg
    28981 cgcgcgtcct aaaaatgacc gtggcggtgg acgccggcac catcatcaat ccacaggctt
    29041 ttgaaggtca gctggaaggc ggcatggatc agggtgtcgg gtatgccttg cgggaagaat
    29101 acatcctggg aaagaccacg gattatgttt ccttcaaatt cccaaccatc aaggatagtt
    29161 ttgatgtggc cgcgatcatc tccaaaaccc ctcgcagtaa cggcccgttg ggcgctactg
    29221 gcgtcgggga aatgaccatg gtatcaacag ctccagcagt taccaacgcg atttaccatg
    29281 cttgcggcgc gcgtgtgtac gctctgcctg ccacaccgga aaaagtgttg aaagcgctgg
    29341 cggcaaagta aggagacgag gttttgagcc ctgcccgggc ttcgcgcggt ggagtttcca
    29401 gagcttgttg agcgtgcaag cacggacggt gtgaggccgg gcaaacgggc ggcagttcaa
    29461 gggaatcgat gcatggaaac cacaggtcct atagagaatt tccgggctga ttttccagcg
    29521 ctgcaccaga ccaggaacag cctgccgcct gtttactttg acaatgcctg cacaacgctg
    29581 gtaccgcagc cagtcattga cgaactgaca gcgtattaca cggaataccc ggcttgcggc
    29641 gggcgcagaa gtcatcattg gtttgcctcc gaagtcaccc ggcgtgtaaa cgctgcgcgg
    29701 gagcgcctcg ccgggtttat taaggctgaa tcatcctccg aaattatctt taccctgaat
    29761 accacccagg caatcaacat ggtggcgctg ggcttgcctt ttaaggcagg agatgttgtg
    29821 ctgctctccg gagaagagca caactccaac ttgctgccgt ggctgcgcct gcagcaaaaa
    29881 ggcgcagtgc gcgttgagca cctgagcgcg gcggagagcg aagaatttga cctggcggcc
    29941 tttgaggaac gactgagcag ggcaccagtg cggctggtca gtctggcgtg gacctccaac
    30001 gtaaccggca gcagcctgcc ggcacaggaa atcatcgccg cggcgcacag gcacggcgcg
    30061 cttgtattgc tggatggcgc gcaaactgtg ccgcatcagg cggtcgatgt gcgctctttg
    30121 gatgttgatt tcctggcgtt ttcgctgcat aaaatgtgcg gtccgcgcgg ggtgggtgtg
    30181 ctttatgcca ggaacgaatt tatctccgcc ccgagcggca gcccaaacgc actggaagca
    30241 gtccttctgg ggggcgggaa tgccattaac agtacttttc agggctacca actgctggaa
    30301 ggcccggagc gctttgagcc cggcattccg gattatccgg gtcagattgc cgcaggcgcg
    30361 gcggtggact accttgagca tgtcgggatg gagcgcatcc gcaagcagga agaaagcctg
    30421 aacagctacc tcagcgcgcg catgcaagaa cgctacggtc atttgggctg gttggaaatt
    30481 ttgggaccgc tggagccgga acgccgtgga gggattctca gctttagtgt ccggcgtcca
    30541 aacgcggttg ggattgcgga agagctggac cgagccgcta atattatgat ccgcgatggg
    30601 gtcttctgcg cgcactcgta ctttaacgag cgctacggcg aagcctggct gcatccaaaa
    30661 acgcatcggg agcaccgcat ggtgtatcgg gtatcgctgt atttttataa tacccgcgca
    30721 gaatgtgagt ttttcctgaa cgctctggat gcggtttttc aggaacgcgg atacctggaa
    30781 taggcttcgc gatggatgag attgttatcc gtcagtaccg gcagctctta aggcaggatt
    30841 ttccgcattc cggagagctc aagaatccgt cggttttcgt ggaagcagtt ggcgagaact
    30901 tgattcactg cggaaacacc ggaaacttca tgcagctgta tctgaaaatt gaacaggaac
    30961 gcatctcggt gatgacttat ctgtgttcct gtgaacccac cgccaacgtt gcggttgagg
    31021 ccttatgcga aatggcgcag gggaagacgc tgggagaggc tgaaaagatc agctgcgagg
    31081 atttgctgca agctgttggc agtaaggatg aggagctggg actgaaaagt gccgggctgc
    31141 tggctttact ggggcaggcc atccgggaat atcagcaggg aaacgcgggc gggggaggac
    31201 aggcttccga cccgggccgg gagcacataa gctgggacaa tacgctttct ttatagcttc
    31261 tccccttctt gaaattccgc gcgtcaggct tcccttctga ttccaacgtg gggttgcgtg
    31321 ctgaaacgct gaaatcagac ctgtgctgcg cggaatagcg gcgcgggatt cttgcgcgga
    31381 cagaccttga ccttcaaaca cttcagcgcc ggcttggctt gggctatccc ccaaaacgcg
    31441 cggtccagct cccggaggga gtgccccgag ctgccgcccc gcagctgcag aacccccgcg
    31501 ggaatccctt tagcgtgccc tcaggaaatc tcgcagcccc ctgaaaaacc tcccgttcat
    31561 cattgttacc aacgccagaa gctttccgcg gctcactcct ccacccatca tcagcgctgt
    31621 gcgcagcggc atctcctgca gcatggcgtt catcatctta ctggttgggg tgtcttgttc
    31681 cggtccgttc atcttctgca tgcccttttc aaaggaacgg aacaggtagg cgccgaccaa
    31741 ggtgcttttc atatcggcca ggggggtatt caggctgaat tgaccctttt tcgttggcgt
    31801 attgcctggg agaggcgagc ccataagcgc ctcaaagctt tctcgcggga agccgacggc
    31861 ctgcgtgggg cgaaagtaag gctccaggcc ttctgttctg ggcgcgcgcg gcaggctgct
    31921 gccgggcagt tggattagcc cccgcaggcg gatatcccgc gaggatgcgc cgatgagaat
    31981 ctcgaaatca ccgccttcgg tcgcccattg gccgctttcc gcgtcataaa aggcgaacgc
    32041 gcgcgggtcc agttccatct caagctggcg ttcctcgccg ggctgaagtt ccaccttgag
    32101 gaagcctttc agttctttct ccgggcggaa aactgaagat tccagatccc gcacgtagag
    32161 ctgtgcggtt tccttcccgg gtcggctgcc gcggtttttc aggctgaaac tggcgatgac
    32221 atgctctccg ctgatgcgta gggtcaggcc gctgtattca aaagcggtgt agctgagccc
    32281 gtgcccgaac gggaacagca cttgcgcgcc gaccgcgtcg taatagcggt agccgacata
    32341 gatactctcg cggtactcca ccgtttgcgg accacccggg tagggttggg ctggcgtgtc
    32401 tgccagcctg atggggaagg tttcagcaag cttgccgctg gggttcaccc tgccggtcag
    32461 gatatcggcg acagcgcccg cgccggcctg cccgcccagg taggcttcca gcaccgcggg
    32521 ggcggcagcc agccagggca tttccaccgg cgaaccgttg ctgagcacta cggcgcagcg
    32581 cgggtggacg gcgcacacgg cggcaatcaa tgcatcgtga ccggcaggca agcgcatgtg
    32641 ctcgcgatcc agcccttcca cttcatccat gtcggtcagg ccgacacaca gaatgactgt
    32701 ttcggcgcgg ctggcgaact ccaaggcttc gcgcaggagc gcgtcatccg cctggccttg
    32761 ttcggtgtag ccgggtgtgt agagcagccg ctcttccccg atgagtttgc ccagctcttc
    32821 gtatagattc tccagccggg taggggtaat aagagagctg cctgcgccct ggtaacgcgg
    32881 cgttttggca aatctgccaa tcagagcgac ttttccgccg gaagagagcg ggagcaaacc
    32941 gccttcattc ttcagcagaa cggcgccttc agaggcggct tgcctggcca gggcatgatg
    33001 cgcgtgcggg tcatagctga agccgggctg gtaactttcg ctggattttt gaatcagact
    33061 gagcacccgt cccacagcct ggtccagcac ggcttcgtcc agctctccgg aatgcacagc
    33121 agcgatgagc ttttcagtgt tcccgttggc cacgcccggc atctccaggt cggtgccggc
    33181 gcgcaccgct gcgacaacct cattcatcgc gccccaatcg gtaacaacca gcccctcaaa
    33241 gccccaatct tccctgagga tgtcgctcaa cagcgttttg ttctcgcagg cataggtgcc
    33301 gttcaggcgg ttgtacgaac acattaccgt ccagggctgc gcgtctttga ccgcgcgttc
    33361 aaaaccggac agatagattt cgcgcattgc gcgtgggtcc actacggagt tggcggtcat
    33421 gcggcggctt tcctggttgt tcagcgcgaa atgctttagc gagctgccga cccccaggga
    33481 ctgcacgccc aggatgtggc ttttggcaat ttcaccggtg aggaaagggt cttctgagaa
    33541 gtattcgaag ttacggccgc acaaggggga gcgtttaatg ttggcgccgg ggcccagaat
    33601 gacgctcacg ccttcctgca ggcattcttc cgccagcgcc tggccgacct tgaagaccag
    33661 gtcgcggttc caggtggcgg ccagcgcgga ggcggtcggg aagcaggtag cgggcacgct
    33721 ctggttcaac ccgacatggt ccggcgagtc ggcttgcttg cgcaggccgt gaggtccatc
    33781 tgtcaacatg ataccgggga tatcgaggcg ttccaacgct ttcaggaacc agaaattttg
    33841 ccctgaacac agggcagctt tttcttcgag tgtcagttgg ttgatgaggt cagggattgg
    33901 ctgcatggga gttgtccttc cgggcttggg atttatggaa ttataaagga gagcagccgg
    33961 aaaatccgat ataagcgctg aatttcggga agatgccgtc cctctgctga gtgcaagcac
    34021 tcttgcagat aaaaaaaccc gtccgaagcc gggcgggtga agatttactg cgaccttccg
    34081 cgccagtacc cgagggttct ttcgcacgag ctggtcaggt gtgagcgcat ggcctggacg
    34141 gctttttctc tgtcattgga tttgagatag gtcaggattt gcatatgttc gcgcactgcc
    34201 acgtcatatg agcgcggctg cccgacaggg gttccgcggg cccactcgat taatccgcgg
    34261 atatcctcca tgattgcgac aagtttttta ttgtcgcaat acttgaggag ctgctgatga
    34321 atgtggtatc gtctggaagt taaacccgca gcaacccgcg aaaaccgcgc acaccgtagt
    34381 aggattccgc gctgttgtgg tagaaaaaga cccgcccgta gcgccggtca ccgaacagag
    34441 cgccgcccag cttgcgcaca ccctcggggg tgaggaccca actggaggtc ttcaggtcga
    34501 attcccccag ggtctgcagc gcactgtact catcttcgtt tagcaggctg atgcccattt
    34561 cggcagccat ttcaagcgcg gagccggccg gcctggcggt tttccgggct tccagcgctg
    34621 cgcggtcgta gcatagattt ctgcgcccca gcggcgtctc agcggagcag tccgcaaaga
    34681 ttatttctcc actttccgcg tctttcccaa ccgcgtccgg ctcgccgccg ctggcttcca
    34741 tcgcctgcag tgaccacatt ttgccgggct cgcttgccag ccttacgcgg acatccgacc
    34801 aggcaagccc ggggtgacgg cgcggattgt gctcaaagcg ggcttgcaga acgttcagga
    34861 atgtttcggc ttcctcttgg agcaggcggt gttcattggt ttggtctgtg ttcatacgat
    34921 aatcctatcc ctgcagggcc gcggagtcaa aatcctgaaa gaagaaatat tatacaaaag
    34981 ggctgccgcg cagaggttgc gcgtagatat tttgattcct tgcgcttcct gataaaagcc
    35041 tggcggcggc cgggcggact tttcagacca ttagatattc ccaaattgtg catggagact
    35101 caagtaaatt cgatgcatca cttgatgatg gcgcccaacc atgacgaaca tttaggctgc
    35161 tgtttttgta aaggctaaac aggaatatcc tgatgcgggc aattcagaca tctcctcttt
    35221 cgtggatgaa atcgtcagga gcattttttc ctcgaaacga agccccttct ttcttctttt
    35281 tgatcctgtt acaagcttgc ccgaaccttg acttttcccc agatgcaagc cgctctattt
    35341 tgcctcggtc cagctgctgg gacggggagt cagcggggta agcctgctat tcctttgtgt
    35401 gccggggtgt tcatgttcct gcgaaaaatg catggaagaa tcgaatgata atttcatagc
    35461 tcctaatttt aatcaggaca tttctatttt gctactttag gacattatca tgttgctctt
    35521 ccatttggag cgctggtttc ttgcggaaag gcggcttgga tgtataattc tcttcctgta
    35581 accaaccttt gcactctagt ggacaggctt gggattgctg tcctaaaacc cataatccca
    35641 ccataacaga attccatcca ctccaaaatt ccataccccc caagaaggca gccggttacc
    35701 tatgcagtct attgctcgaa cgaaaaacag aaattttctc cagaacggcg ctgccgccat
    35761 tcttaaaaac cgctggtttt atctcggcct caccgtcctc atcaccagca ttgtactcat
    35821 ctttacgctg gaaatgcagc tttacaccgt ttcactggtt tccgggctgc ttgggattgc
    35881 tttggcggcg tatttcatcc tgcgtatcac cttacggccg gttacaacgc aggaaatggt
    35941 ggaagtcgcg acggatatcc gcactggcgc cagaacttac ttgcgaagac agataaaaac
    36001 cattctcctg gtcactcctt tctttgccgc cttgctgttt tggatattgg attggaaggc
    36061 agcccttacc gcggtttttg gcgtactgac ctcgctaatg gcttcgtaca tcggaatgag
    36121 cgtgagcgtg cgcaccaacc tgcagactgc ggatatcgcc cgggaaggca aacgcgagcc
    36181 cttccggact gctttgttcg gcggctccgt gatgggtttg tgcatcacag gactgagttt
    36241 gattgtgctt tctgtgctct tcagcatctt ccgggatccg aacgcgctga ttggctttgc
    36301 cttcggcggc gggttggcgg cgctctttgc ccagattggc ggcggcattt tcaccaaatc
    36361 cgcggatatt ggcgcggacc tggtggggaa gattgaagag aatatccctg aggacgaccc
    36421 ccgcaacgcg gcggtcatcg cggacctggt aggcgataac gtgggcgact gcgccggccg
    36481 cggcgcggac ctgttccaaa ctttcagcga tgacctggtg accggttcga ttgtggcggt
    36541 tgcgatggcc tcggtttacg gacccaccgc tatctttttc cccttcctgc tgcagagtgt
    36601 aggcgttttt gcctcggctg ccggcatctt tgccagccgc gagtggaaac cgggcatgaa
    36661 tccctccacc cagttcaacg tcggcctgtt tgtcagctcg gtgctcagca tcatcggcgc
    36721 gctcggcatg tccatgctgc tgcttaatga ctggataatc gggattgcgg cgaccctggg
    36781 cgtgctgatt accacggttg cttcgcttag cacacgttat tatgccggca tggagggcaa
    36841 acccgtgcgc aacatcgcca aggcctccaa ccgcggcgcg gcgctgaacc tcatcaccgg
    36901 catctcgtac ggcctgcaaa gccccgtcct gccggtcatc gttctgatgg gcgtggtcat
    36961 cggggtatat accctttcag gaaacagcct gctggcactg gtggcagtca atatcggcac
    37021 ggacctgctg attaccttta tcatggcagc cgacgctttt ggtccgatag tggataacgc
    37081 tgctggcatt gccggcatgt cacgtgctcc gcagcatgtc atcgacaaac tttcccatct
    37141 cgactcggtc ggaaacacga tgaaagccgt caccaaagcc tacgcgatga tctccggcac
    37201 aatcaccgcg tttgtcattt tcctgacctt ttacaaactg gcgggcatcg acgcgattga
    37261 cgtgaccacg cccttcaacc tgagtttcct tttcatcggg gtatgcctgc ccttcctgat
    37321 ttcctcgctg gtcattggtt ccactgccaa aaccgcggaa tccatggtgg acgcggtgcg
    37381 cgatcaattc cagcagaacc cggacattct gaaggggctg gcgaagccga attactccca
    37441 atgcatcgat attgccacca gcaacgcgct gcacgagatg attttgccca gcgctatcag
    37501 catcctggtg ccgattggga ttgggttcgc gtttggaatg aacgcgctcg gggcggtttt
    37561 ggttggttcg gtggcgtgct cggcgctgtt ggggcctttt ttcaataaca ccggcaccgc
    37621 atttgacaac gccaagaaga tgattgagaa cctggagcac tcgcgcggga cgccggagca
    37681 cattgccgcg gtgattggcg ataccgtcgg ggattcgctc aaggatgtgg ccggcccgtc
    37741 cacgctgatc ttcatgaagc tgattggcat ggcctcgctg ctgattgtgc cgctgattaa
    37801 gtagggggag cagtgaatac aaacgaataa acaggagcaa aacaccatga accccaccaa
    37861 aaaggcaagc gaaatgtccg tccgggagct ggcggcctat atcgaccaat ccgtgctgaa
    37921 acctgaattc acccaggccg aaatccacag gtacatccag gaaggcgttg actttggctg
    37981 cgcgactgtg tgcatcaacc ccgcgtcgct ggacattgcc gccgaattga cccgcggcac
    38041 ggacacgaag atctgcgtgg tgtgcgactt tccgttcggg ctctccacca cccgctcgaa
    38101 agtgatgcag gctgaggagt actgccgcag aggggatgtg gccgagctgg acgttgtcgc
    38161 gaactacggc tggatccgca gcgggttgtt cgccgaggtg gaagccgata tccgcgcggt
    38221 ggtggacgcc tgtcaccgct gcggcaccgc ggtgaaggtc atctttgaga ccgacgcgct
    38281 gacgttggac gaggtccggc aggcttgcga ggcgggcatc gccgctggcg cggacttcct
    38341 gaagacctct actggatttt tcaccggcgg gaaaagcgat ggagcgacca gtgaggtgat
    38401 cgcgctgatg atggagacgg cgcgcgggcg ctgtaagatt aagggctccg gcggcatccg
    38461 cgaccagcgg cacttcttcg agctgattga catgggcatc gaccgcatgg gcatcggcta
    38521 ccgctccacg ccgctggtgc tgggagtgag cgcggaagag gccaggaggc tgcggggctt
    38581 ggggtaactc tctgaggccg cgccgcatcg atgcgggcgg atcggggaaa acgaaaacca
    38641 gtcagcctgc gcaaccaagg caacctacct ccattcctgg ggagcgtact tcagaggcaa
    38701 atcccagccg gcattgtgtg gttgagccct aagctgccgc gcaataattc ctatggcttt
    38761 gccgtgcagc ccggtggaca ggaattgcag cacatataag atcccccccc cggtccaaat
    38821 aatcacagaa taaagcatgc ggcgtaaagc gtttcgctct ttcctgccct ttttgacctt
    38881 cctcgcggga tgtcagctcg cttccccgcc gggcactgtt caggcgcctg cagtggaagc
    38941 tggcacaagc acggcattca ttcccgataa cgcctgcgcc gcggcagttc agcgctattc
    39001 gatgagtatc tgtttatccc ggcttcgcca ggtcatggtt tcatcgctat ggaatatcag
    39061 gtcttgtcaa cgatggttgt cgtcttgaca attcccaatt tcaacaatac cattaatcaa
    39121 ttctttggtt attttggaag tttatgaagc ttacgcgtcg tcaggaaact tttattctct
    39181 gtctactgga cttgtttcgg gagtataagc gtccaattca ttattcaacc ctggccgaga
    39241 gggttggagt aagtcctttt actgcttatg acatgcttcg cctccttgaa gaaaagggct
    39301 ttgtgacttc cgagtatcgc attgagcctg ggaaaagtac ggtgggtagg tcagaaattg
    39361 tattccttcc aacacaatta acctataacc ggtttgcgga attggcgaat ggcacggatc
    39421 cggacgattg ggaaatcgca aaatcccaaa ttttgaagag aattctcgct ggcggcatcc
    39481 atgaccgcga actggctgat gaattaattg caaggattcc aactgatgag cctgagacgg
    39541 tccggtattg ctttgaagtg attgctttgc tgtttttacg tctcggcaaa aattcgggac
    39601 ggaaggttct aattgagaga atgccccaaa tccttgcgtg gaaagagtct ttctcaaaat
    39661 cagggctcat cattttggga ggttttattt taggcctgtt ggcgacggat aatccgttaa
    39721 ttttggaagc cgatcagcca atttttgagt atgtccaaag ataccaatct ctggtctcgg
    39781 aaatggaacc tcgattggcg aaccatttgg ccagcaccat ttatgacatg ttttcttcgg
    39841 tgctgaaagc ataagaacct ggtctggtag aaaatatctg atgactaaca caatcgaagc
    39901 gattcatcta accaagcgat atccttccca gccaaaacag agccgtccag ctttggacaa
    39961 ttacgacctg caagtaaaag ctggccaact ctatggcctg gtaggaccag atggagccgg
    40021 caaaacaacc ctgcttcgga ttttatccac cgtgctctcc ccaacttcag gttctgctca
    40081 tatcgaaggt tttgatattg tcaaacaatc cgcgcaagtt cgctcacgaa ttggatatat
    40141 gccccaggca tttagcctat atccggattt aagtgtttta gaaaaccttc gtttttttgc
    40201 ggacatgaat ggcgttccca gcgaaaaaca aaaacagcgc atcccggaat tattggaatt
    40261 tgcccgactg acagaattta catcccggcg aagtgaaaat ctttccggcg gtatgcgcaa
    40321 aaaactggct ctggcttgcg caatgatcca tgaacccgaa attttacttc tggatgaacc
    40381 cacaacggga gtagatccgg tttcccgtcg ggaattatgg cagcttttag caaaagtcat
    40441 cgaaagggga gttactatcg ttgtcagcac gccctacatg gatgaagcag agcggtgtga
    40501 tgttgtcagc gttatctatc agggaaaaag tctaatctcg gggtcgccaa tggagatggc
    40561 tcagcacatg cctttcaaga tagttgaagt caaagcaagg ccgagaggta ctctgcggca
    40621 ggtggcgggc aatctcgatg gagtgattca atggcgcgcg gttggtgatc gtttacgtct
    40681 ttctgtggcc aattcaacag ccgtccagga aaatttaaaa gccgcattaa ccaaggcagg
    40741 agctgaaatc acaattttac gtgaagcacg aatcaccatg gaagacgtat ttatctatct
    40801 ggtcgaaaaa caggagaccc cggtatgaac cgtgaaaatt ctgttgaagt acatcagctg
    40861 acacgcaact ttggcgaatt tactgcagta gatcacatca gttttgatat tccaaaaggt
    40921 gaaatttttg gcctgctggg tccgaacggg gcaggaaaga caacgaccat acgaatgctc
    40981 tgcgggttgt taaaaccgac tgaaggagaa gccagagtgc ttggctttga tgttgccgac
    41041 cagtcggaac aggtaaaacg cagaattggc tatatgtcgc agaaattttc cttatacaac
    41101 gacctgacgg tgtatgaaaa cctttggttc tacgcgaatc tttatggcat tccgtctgag
    41161 cgcgtcaaaa tgcgcattac cgaattgatt gaaatggccg ggttgcgcgg gcatgaaaaa
    41221 caattgacgc ataacctttc aggcgcctgg cggcagcgcc tggccttagc ctgcgccatc
    41281 atccacgaac ctcccatgtt gtttttagat gagcctacgg ccggcgttga tcctgtttcc
    41341 cgacgggaat tttgggagat gatttacacc atggctggaa agggcgtcag tgtattggct
    41401 accacgcatt atatggatga ggccgagttc tgtaacgtga ttggcatgat gtatcactcg
    41461 cggctgattg ccttaaatga tcctgacacg cttaaaatta atatgcaagg atatttatat
    41521 gaggtggatt gtgatgcacc tggaagggct gaacagattt tgaattccct ggtagaggtg
    41581 gaggatgtcg cggcttatgg cgtgctcttg catgtgctgt tatcaaaaga ttccgataag
    41641 aatctgatcc tcaggaagtt atctgaaaat caaatccagg tcacccgaat ggaaaaagtc
    41701 cagccctctt tggaagacgt gtttgtttct ttagtgaatc aagagaatcg ccagcaggta
    41761 cgggcggaaa tagaataagg aggttcaggt gagacagagt ttccggagaa tgttcacgat
    41821 agtccgaaaa gaatggcttc atattatccg cgatcctcga acccttgggc ttgtgatcat
    41881 catgcccgtt actatgctct ttttgttggg ttatgctgta gcaaacgatg taacggatat
    41941 acccatggtt gttgctgatt tatctcaaac ggagagcagc cgcaccctgg ttgataagtt
    42001 gactgtaagc ggattcttca aatacaccta ttcagcccaa agcgagaacg aaattctcca
    42061 actgctggat gaaagcgccg tgaaggttgg tttatacatc ccggaaaatt ttgggcgaaa
    42121 tgtcagcaca ggggaaacag gaacaatcca gttttatatc gatggatcaa atccgacggt
    42181 tgcccaaacc gcgcagttgg cggctgaaac catcagtcaa tcgatgtctc aagaaatatt
    42241 gattaaacgt cttgagcgaa gtggttccgg tatgcaatta aacctcccgg ttgatgcccg
    42301 tatgcgctac ttatataacc cggatatgaa gcgcatgaac ttcatgatcc caggtctggt
    42361 tgcgattatc ctgcaaattc aaaccctgct gctgaccgct tttgccatcg tgcgggaacg
    42421 tgaacagggc actctggaac agcttatcgt gacccctgtc cgctcatggg aattgatgct
    42481 cggcaagatt ctaccctttg ttgtggtagc ctttgtaaat gtcatcatga ctgtttcggt
    42541 tggggcaatc tggttcggcg tgagagttgc cggcagtatc atactcctgg ggatattaag
    42601 cctcatcttc ttgctcggtt cgttgggttt gggcgttttc atttcgaata tttcgaaaac
    42661 gcaaatgcag gccatgtata tggcgtcctt tatcatgatg ccttcgttta tactggcagg
    42721 tttattattt ccacgggaaa acatgccctg gctggcatat tatgcgggtt acctcttacc
    42781 tgtgacctat tttctggaaa tcgttcgcgg gattatcctc aaaggggctg gattctccac
    42841 tttatggatg tggatttggc caatggcagt ttttagcatc atcgtctttt ttgccagcgt
    42901 tctgatgttc cgaaagcgac tctaagtcat acgttgtgac caaaaaactc ataaaggagt
    42961 tgtaagatga aaagacaagc ttatctattt ccctctttgt ttcttagcgt agcaatttta
    43021 ctgactggct gccaggcgat caatgggaat gctgccaatg aacttaaggc gtcagggatt
    43081 atttcagcta cggagataaa tattgcctcg cagcttggcg gcacggtggt ttccatcgag
    43141 gtgagggaag gctcgcaggt taaaaaaggg gatgtgcttt tccgtttgga tgatgcctta
    43201 cttcaggcgc agcgcaacca ggcttcagca gcggtaaacg cagcggaaaa ggctgtaaat
    43261 tcggccagga ttcaatatga tatcgcggtg aacagtgccc ggctgcaaga acagcagaac
    43321 cgtgtcaaca gctggaaaat cccgcaaccc aaggaattcc agcttccaac ctggtatttt
    43381 gacaaagggg agaaaatcaa cgctgccaaa gctgaggtag acgcggctgc tgcagatttg
    43441 aaaatagaaa aagaaaatct tgaaaaagtt cttgcggata atacttccca ggagttttta
    43501 tccgccgaga agcggttggc ggaagcccaa actgcgttta taattgcaga ccaggtgcta
    43561 actcaggctg atgctgccca ggacaaggag gatatttcca actatgcgaa tgacctttat
    43621 gatatggcaa aatcagagtt gagttccgct caaaccgaat acaagcgtct tgtaaccact
    43681 caagcagcgg ctgaagttct ggaagcgcga gcgcgcgtcc gggtggcgca agaaagattg
    43741 gacagggctg tggattatcg aaactctctt gaaagcggcg accaatcgtt aatggtccgg
    43801 gcggcgcttg cgggcgtaca gcaggcggaa gcagtattga cgcaggctca ggcggcccag
    43861 gcgttaatcg atatccaatt ggaaaaatca ataatcaaat cccctgcgga tgggattgtc
    43921 ttgacccgaa atttggaaga aggcgaaatg ctctctcccg gaagtgtagc gataatcatc
    43981 ggccaacttc aggaagtgag ccttacagtc tacattcctg aaacggaata tgggaaaata
    44041 aagttgggtg atagtgtctc gattgcagtt gattccttcc caggagaaaa gtttagtggg
    44101 attgtttcct atatttcgga tcaggctgaa tttacgccgc gaaatgttca aacgattgat
    44161 ggccgtaaaa cgaccgttta tgcagtgaaa attactgtac ccaattcgaa tctcgaactg
    44221 aaacctggca tgcctgcaga tgggaccttt aactaatatg aaaactcaaa attctaccgt
    44281 tgtcgtaact ggcagcgccg ctcaggtgca gccggaaatt gctgctcaat tgggaatcgt
    44341 gattcttccc ttgattgtcg tcgttgacgg gaaagaacat ctggacggtg ttgatatttt
    44401 tccgggtgag ctttaccaga aaatgcgcac tcaaaaaata gaagtaaaga ctgccgcgcc
    44461 caacgtcggt caatactatg aatgttttaa gagaatcata aaccaacaag gttctgatat
    44521 tctttgtctt actctgtcca gccaactgag ctctgattac aatgcggcag tagaggcggc
    44581 aaagatgtta tccgaagaaa accaggaaaa ccgtgtaacg gtctttgaca gcttgagggc
    44641 tgcggctccg caagggctgc tggcagttga agcggccaag cgcttgcatg caggggaaac
    44701 gcttgaagac accgtccaat atctgcagaa tgccaggtac aagtccggac taatcgcagc
    44761 tttggataca ttggaatatc ttgccaaagg tggtcgaatt gggaaggcgg catacctggt
    44821 tggcagcgcg ctgcaaatca tcccaattct caccatcaac aatgaaggaa ttgtcgcgcc
    44881 ttccacggtt atccgaaaaa aaagaaacat cattcctacc tttctctcta ccctggccag
    44941 gaaaacggcc ggatataagc gcttgagttt atcggtgatg catgcggatt ctgttgacca
    45001 ggtcgaaaca ctgcggcacg tgttaaggga acagtacccg acattggaaa ttccgatcag
    45061 cgagttcaca cccgtcatgg gagcgcatac cgggccgggt ttgcttgggt tagggtattt
    45121 ttttgaataa aagtttgaaa aggaaagagg aagcccatgc tcgaaaaaat atcacaaagc
    45181 atgccagcag tgaccatatt ttccctcctg gtgtttgcgt atcttctcgg ttcgataccc
    45241 agcggctatc tggcaggaaa atggctgaaa ggaatcgatt tgcgggaata cggcagcgga
    45301 acagtgagcg gttccatggt ctgggagcat gtggcaaaat gggctgtaat gccggtaggg
    45361 ctttttgata tctttaaggg cgctttaccc acctggcttg gtttgcgcct ggggctgggg
    45421 gaagtgccag ccatgctggc aggaggcttc gccatggtgg gtcacaattg gcctgtttac
    45481 ctgaattttc agggcgggcg tggttttagc ccatttttgg gtgaattgct ggttcttttc
    45541 ccctgggggg taatcatcat tctggctggt ttggtgttgg gcaatttttt tcattccgca
    45601 gctgttccat tgtttgccgt tattctcctg ccaatcttca ccacctggat gggcggtcca
    45661 tctatcatac tttggtgttc catgatgatg gtgttgatta ctttgataaa aagggtagaa
    45721 gccaatcaac ggccgctgcc agccaaccca accgaacgac gcgaagtaat cctccgacgc
    45781 atatttttgg accgtgatat ccaggatcac caggcctgga ttcgcaggga gccaaaagag
    45841 tagcccagaa agcgtctatg gattttttac ctgaacatgg ttcctgtttt gtttgtggta
    45901 agtcaaatcc taagagtatt gggattgcct ggcaattatt agcgggaaat catattttag
    45961 cgtcctttgt gttcagtgat tctcaacaag ggccgccagg atatgttcat ggtggagcct
    46021 cggcggctgt tatggatgaa gccatgggac tggctgtatt ctattccggc tacagagcgg
    46081 tcactgcgaa catgtcggtt gactatcgca aaccgatacc cttggggcag cgcgttacaa
    46141 tccaggctga aatgagcggt aaaaccgaaa gacgaatcct tgccaggggt gagattttat
    46201 taccggatgg ttctgttgcg gtttctgcca cgggaatgta cgcagaggca aaactttttt
    46261 tcgataagat cgcaggccaa ttgttggagc caaaagaatg aagaggctgg tttcgaagat
    46321 catctgggtt gctgtttttt caatcgctat ggcgtacctg gaagccgcta tcgtcgtata
    46381 tttacgacgt ttattcggca ttggcgacat gatccttcag gttccaccgt ttgaccagca
    46441 aatcgctgca atcgaattag gaagagagct ggcaaccctt atgatgctcc tggcaatagg
    46501 atggattgcg gggcacaata ttcaagcgcg tttgagctac acactcatcg cttttggtgt
    46561 ttgggatatt ttctattacc tttggttaaa cgttttcatc ggttggccaa aaagtttctt
    46621 ggaccccgat ttactatttt taatccccct tccctggtgg ggtccggtgc tctctcccgt
    46681 tttaatctcg atattgatga ttacaggagg ggtggcagtg ctgaaagaaa acgagaccaa
    46741 agcccccatc cattttccgc tttctttttg gattctgctc atcccgggtg ttttgttaat
    46801 gctttatgct ttcatggcag acgcgattcg ggttttaccg gcatccgctg ctgtgctaaa
    46861 cagtttaaga ccgtcccaat ttaattggcc agtctattta atcggactgg ttctggcaat
    46921 cgtttcagtc ctgcaaatca taaaaaggaa aaatgcggtc taagcagacc aatgcatttt
    46981 tcttttgcta aggagctacg gtgaaaattc gttaccttgg gcattcatgt gtagaaattg
    47041 tcggcaggca tcatatcctt atcgatcccg attttacgcg ggaacccctg ccagacgttg
    47101 agtatatttg tgtttcgcat gcccataagg accatctcgg taaggtggct cagattccta
    47161 ccggtatcgt attggcatct caagatgtgt gcgagatcgc gttgaaaatg ggtgtgcctt
    47221 cttctcgtct tcaacccgta agcgtcggag agcatatcga caacatcgaa atattgcctg
    47281 gttttagtgt ggttaaggac ccggtttatt tcatctttcg tctacttttt cgtttttcga
    47341 tccccgatcc aggaggaacc ccgctctcgt tttttttatt caggatgatg cagacctact
    47401 ccatgttggt gatgctcatc aatttccgct ggaggttcat ccggatattt tatgtcttcc
    47461 atggcgcgct tcgcagttag gggctgaaaa atacaaatca gtagtcattg agatggccag
    47521 gaaaatagcc gcgccttata ttctccctat tcattatgat cttcatggca ccgaagcgaa
    47581 cccgaaggaa ttggttcctc gccttgatgc agtcatcctg aacggagaag actggcatac
    47641 atttgagaat catttgttat tggagaattg aaatttccct ttgacttatt attttccgta
    47701 tacccgggag ccatcccttt tggttgggga aggtctccgg gctggtaaac ggcatggata
    47761 tccgaaaagt gggcatggat atacgggagg cacctctggg aagcgggttg atggagagta
    47821 ttgattgata gattttagca agtttaacta cgtgtgagag acaaggctta ctaaaaagtt
    47881 tgggatcaaa aaaccgtagc cccagggact acggttctcg ttagtagcgg ggagtggatt
    47941 cgaaccactg acctccgggt tatgagagat tttcc
//
LOCUS       DAOQAD010000004        45746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_663_length_45746_cov_5.400867, whole genome shotgun sequence.
ACCESSION   DAOQAD010000004 DAOQAD010000000
VERSION     DAOQAD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 45746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45746
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_663_length_45746_cov_5.400867"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="PKL60_00695"
     CDS             <1..1989
                     /locus_tag="PKL60_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNW94682.1"
                     /translation="GGIYPVNPSAVEILGFKCYPSVTDIPYVVDAAVITVPAKSVLDA
                     TKLCGEKGVKGLIIITSGFAEVGEIELEHELVRVAKSYGMRILGPNIVGTLSNSDKMN
                     ASFAPFLPLPGKASLVSQSGALLIAMDAGTYTRRVGFDKMVSIGNMSDVNFSEMVSYL
                     SEDPDTNCIALYVEGLRDGPKFIEAARNCKKPIVVLKSGVSAHGAAAAASHTGSLAGA
                     AKIYGTAFKQAGVTQATDLNDLFDRTLVLSLQPRMKGDNLLILTNGGGVGVLATDSAE
                     KYGLPLHFAPADVQEEMKKHMPDYGSAKNPVDMTGMAGNDWFYETTRSAYSHPWVDGL
                     VVLYCETAITLPEEIADAIYRGIKDSGVTDKPVTVSFVGGERSDKAMEWLVERGIPAY
                     NAPDVAVKAIATLRDDQRMQEMNHNGGYEPGDINSAFAREIIAGARAKGRDALTEVEA
                     KQVFRLYGLPVTTTELASSEEEAVKLAEKVGYPVVMKIVSPDILHKSDAGGVKVNIKN
                     AEQAKEAYRTILENAHNYKADAFIDGIAVQEMAPWATEVIIGSVNDNTFGPTVMFGLG
                     GIFVEVLKDVTFRVAPISLEEALGMEDEIRSAAILNGTRGESARDKDALARVLAAYAY
                     MVYDLKDEIKESDANPVIVYENGQGVKVVDARIILTTK"
     gene            2080..2856
                     /locus_tag="PKL60_00700"
     CDS             2080..2856
                     /locus_tag="PKL60_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNW94683.1"
                     /translation="MKEKKNYLIDMDGVLVHGANMIPGADGFINRLIEEKRKFLILTN
                     NPLYTTRDLAHRLQVTGLNIPEERLYTSALATAAFLNNQRPKGTAYVIGELGLSEAVH
                     AIGYTQTTVNPDYVVLGETFAYSIEQITIAVRLITAGARFIATNPDPVGNSEQGLVPA
                     CGAMAALIERATGIAPFYVGKPNPLMMRSALNYLDVHSENSIMVGDRMDTDVLSGISS
                     GMDTVLVLSGTTDRASMEKFSYRPKWVFNSVADINPKDLD"
     gene            2872..3885
                     /locus_tag="PKL60_00705"
     CDS             2872..3885
                     /locus_tag="PKL60_00705"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNW94684.1"
                     /translation="MEPKLTRREFLKLGLIGLGAAAALPARKVFAAEKAPRQVLVGNQ
                     NGVSVHKLPDEDSLIIYQIGYNEIVNVYDEVESPAGPFWNPIWYRVWGGYIFSGDVYE
                     VHYKLNSLNTDIRPTGQLAEITVPYTRAMFYSRLEGWIPEYRLYYGTTHWVMDIVTGP
                     DEKPWYKIKDEKNSVMLAVPSEHLRFVPDEELQPISPEVPLGKKRIEISIMFQRLKAY
                     EYDNLVFETKISSGGLTPIGTYNIQTKMPSKHMGNGYVTSDIRAYELVGVPWNCFFEM
                     TEGIATHGTYWHTNFGTPMSAGCINMSMADAKWIYLWTTPVAAPEDWQKHGFGTPITL
                     TKD"
     gene            complement(3882..5060)
                     /gene="hemW"
                     /locus_tag="PKL60_00710"
     CDS             complement(3882..5060)
                     /gene="hemW"
                     /locus_tag="PKL60_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNW94685.1"
                     /translation="MFSLYIHVPFCVRRCLYCDFITYAGQLEFLPAYANTAANEIQQL
                     GLAAGSERQSADTVYFGGGTPSLLESRLAGGLLEETARAFGLAPSAEISLEANPGTLD
                     PKKLRELRAAGINRLSLGVQSFSDSELALLGRIHSREQAEESIRWAREAGFEQLNLDL
                     ISGLPRQTLEEWQENLKTAVTYKPEHLSVYSLIVEEGTPLEKQIASGRLPEPDEDLAA
                     DMYELTQEFLAQAGYTQYEISNWALAEGCESRHNKAYWKTTPYLGIGAAAHSFAGGFR
                     TENVPGIQDYITRIQQGEANLEFPFSPANLNRSKIQPYTQMQESMLLGLRLTREGVAL
                     SAFQRRFGQDARELFSAEMMRLQEKRLVEFVQFPDGPHLRLTRRGVLVGNQAFMEFVD
                     "
     gene            5364..6515
                     /locus_tag="PKL60_00715"
     CDS             5364..6515
                     /locus_tag="PKL60_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNW94686.1"
                     /translation="MSQEKTTQNTKICPVCGTRLSETASRCLVCGTQLSTTAAVKKGS
                     GIPSRKLPEVRLNLPVAIALGFVFLALAGLLVFFIVKGSPAAPLTPGEVVEATPTLTP
                     TQTITPTQTLTPTPLPTWTPLPPMEYTVRSTDYCSSIAAVFGVSIQSIIRENNLDANC
                     TIKEGDVLRIPQPTLTPSPQPTATSSGIESTESDCQTITIKVESGWTLSSIALNYNVT
                     MASIREYNRMTNDVVYEGLDLIIPLCERKPTAGPTPTPTPAPPYPAPNLLLPSSGAAF
                     SASQDAITLQWAAVADLRENELYRVTVEDLTAGNGRILVEYVRDTRFILPTSFRPLDS
                     TPHIIQWSVAVARQINSGTTNPIYEEAGFLSEKRVFSWIGTGVSVTPTP"
     gene            complement(6520..7455)
                     /gene="trxB"
                     /locus_tag="PKL60_00720"
     CDS             complement(6520..7455)
                     /gene="trxB"
                     /locus_tag="PKL60_00720"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNW94687.1"
                     /translation="MEANTHHQLIIIGGGPAGFTAALYGARADFHPLIFTGMMLYGQA
                     SQTDIIENYPGFPEGIQGVELGKFFEQQALNFGAKVEYDQVLSLNLQSRPFQIQTYSS
                     SYTADSVILAMGAEPKKLLVPGEAEFTGRGVSYCGTCDGFFTRNKEIHVIGGGDTALE
                     EALFLTRFARSVTVIHRRDALRAGAILENRARSNPKIHFLWNTIVDEIRGDEKVHSLL
                     VRDVITGQKREIPSEAVFIFIGNKPNTDLIDGQVNLDEHGYVKIDDKMTTNIPGVFAA
                     GEIADPHYRQVITSAGMGAAAAISATRYLENLECQ"
     gene            complement(7467..8816)
                     /locus_tag="PKL60_00725"
     CDS             complement(7467..8816)
                     /locus_tag="PKL60_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW94688.1"
                     /translation="MKSDEILSKSIRHNLWTWTAQRKAEILPVERAEGVYFWDTGGKR
                     YLDLNSMVMCVNIGHGNRHVQEAMIRQIRELTFAGPHMATKARALLGEKLASLTPPGL
                     TRFLYTLGGSDANENAVKIARDYTGKFKILTRYRSYHGATMGAMALTGDYRRLHWEPE
                     TMPGVVHFLDPYAYRSPFFPDGGVDEESFSRAYVDHLEEIICFEGPDTIAAVLIETVT
                     GTNGILVPPSGYLQGVRALCDKYRILLITDEVMCGFGRTGKWFASQHWDVVPDIITMA
                     KGLTSGYAPLGAVAIKEEIAATYDDRQFVGGMTYNGHPVSLAAALANIEVIEQEGLLK
                     NALRRGETLKALLADLKQKHPCVGDVRSIGLFGAAELVRDRASKEPLSPTAKPLSPVM
                     KHVKSYILEHGVFMYNTFQTLLFIPPLIISDVQLREGFDIVDAALSIADHEIQAGME"
     gene            8914..10290
                     /locus_tag="PKL60_00730"
     CDS             8914..10290
                     /locus_tag="PKL60_00730"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNW94689.1"
                     /translation="MTHIENSLKFFTEHTDNFLQQYQDLVRIPSVSTDPEHKADMQAA
                     CKFLADKLRGLGAGHVEVFPTKVHPILFAEMRADAEDAPTILIYGHYDVQPAAPIEEW
                     QTPPFEPTISGEYLTARGASDMKGQVMAALAAVESVLAAGPLPVNLKFLLEGEEEIGS
                     PSLDGFIASHADLLACDIVLNPDSGMVSRDSPTIVYGLRGLLYFEIRVDGPKADLHSG
                     LFGGNVANPANVLAAIIARLHNPDGSVAVPGFYDKVLPLSPEEKQRIASVPFDPQALL
                     QITGVPQLFGEAGYSPLERTGARPTLDVNGIYSGFIGEGSKTIIPAYAKAKISCRLVP
                     DQNPDEIYALIQAYITSLTPEAVRLTIFKHSGAPAYLVDDAPGLEQLSKALAETWKKP
                     LSFKREGGSIPVATTMQTHLGVKSLLTGFGLPDDQIHSPNEHQHLPTWKLGVQALIRF
                     LLSFEHAN"
     gene            10280..11200
                     /locus_tag="PKL60_00735"
     CDS             10280..11200
                     /locus_tag="PKL60_00735"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNW94690.1"
                     /translation="MPTEAAPELKMPSYGGQAVIEGVMMRGKKAVAMAVRSPQGGIVM
                     FDEQLPALYRSIWFKTPFVRGVLSLWDSLELGMRYLTKAANVQTGEDEKIEGAALFFT
                     VLAAVLVGIGLFFLLPALAAGWVDKFLNLGSWWSNLFEGILRLAILVLYMALVGRMPD
                     IKRTFMYHGAEHKTINAFEAGVELTPQNVALQTKVHPRCGTSFILTLVVFSVLIFSIL
                     GPLPLHWRLISRVLLLPVVAGLAYEYIRWAAHAMDKSAFVRALIKPNLLLQKLTTKEP
                     TEDMLEVAIAAFKRMYALEQDTEEAQPLTS"
     gene            11197..11787
                     /locus_tag="PKL60_00740"
     CDS             11197..11787
                     /locus_tag="PKL60_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNW94691.1"
                     /translation="MKTWQSVFLGFIGGLLLSGLILLLILPQRGEPIQLATAAPDQRA
                     RSTPTAAEIEVYVAGAVANPGVYMLPAGSRVHDALSTAGGAAQDADFERINLSAFLQD
                     GQRVYMPRVGEAVPADDTTRGQTGLEAASNLVNINGADLETLMSLPGIGETKAAAILS
                     YRSEHGPFTSLEELLNVPGIGQALLEEIRPLIMLGP"
     gene            11840..12292
                     /locus_tag="PKL60_00745"
     CDS             11840..12292
                     /locus_tag="PKL60_00745"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNW94692.1"
                     /translation="MDNNQKLKADLQKAIKDQDPLRKRVLRLLISTIQLAEVAKGSAL
                     SDSEFVAVVQKEIKTKLDTIADAEKAGRADMVSESRLEIKELEAYLPRQLTEAELVAL
                     AEEIIRETGAASPKDMGLVLKVLIPRLAGRASNQDASRVVKDCLSKPA"
     gene            12295..14415
                     /locus_tag="PKL60_00750"
     CDS             12295..14415
                     /locus_tag="PKL60_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNW94693.1"
                     /translation="MAKNRENGTKPAGFTQGALRLLILLVSSLLIFASLVLPELITRS
                     NIPAGVGEAASQEILAPYSITYESKVLTERARTQAAAAVPPVFLPADPSISRHQLETL
                     DSVLYYISAVRKDNFSTPEQKLQDLAAISSVKLSSAVAAQLLELDEQDWQEIEVEARR
                     VLEQVMRDTIRSDNLSQIRSNLPAVIDFSFRPEDAQLVIALVSPLITPTSLFSEEQTN
                     AAREQARAGIEPISRQIIAGEVIVRRGQIIREEDYEALNVFGLAKPVNQTDQVITSAV
                     LTGVIAVLAALYFQRRQDQPFFSVKALLVIAGLFLIFLALGRFLVVDRTIVPYLYPVA
                     AFGLTLSIIFNLELGIVMSMFLAILMVFDNTREAELGVFYILPAVVGMLTIGRARRIS
                     AFLVAGLLTGIAGMAVVVAFRLGDTYTDLLGLTSLIASALIYGLASGSLALLLQYVVS
                     QILDIPTALQLLEVSRPDHPLLQYILRNAPGSYQHSLLVSNLAEQAAEAVGADRMLVR
                     VGALFHDAGKANNPAFFIENQVKDKLDSHDNLDPATAAATVIQHVHDGVELAKKYHLP
                     SRVIDFIREHHGTMLTLYQYSQAAAAADDPESVDKALFTYPGPKPQSRETAIVMLSDG
                     TEARSRAETPRSDDEIRALIRQTIDMYKNEGQLDATDLTLKDLSTIEDSFFRTLQRSY
                     HPRIQYPTLKPNGSAVSKGSDSAG"
     gene            14440..14901
                     /gene="ybeY"
                     /locus_tag="PKL60_00755"
     CDS             14440..14901
                     /gene="ybeY"
                     /locus_tag="PKL60_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNW94694.1"
                     /translation="MIEFMIPRKYRTVISADRLLLAYQRTVEKLGRRAEGAVTLKITD
                     NAGIRKLNKAYRGLDEATDVLSFENEFVDLETGRRFLGDIAISIEKALAQAEERGFSL
                     QEELEMLFVHAVLHLNGYDHLEEAELEEMNSLQDEILQEIGNPIRGSISHE"
     gene            14894..15715
                     /locus_tag="PKL60_00760"
     CDS             14894..15715
                     /locus_tag="PKL60_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94695.1"
                     /translation="MNETTLKPKHSRGLIVVLLSVIGMLLALLLAVGALASRLTLIDF
                     SAKLGLSIQETLTATNSGLKLISQSLDDVEASFDELQNSMKTLESSVANLSPMLTDVS
                     ELIGTDVSAIASEGATTLKSASESSKLIDSTLQFLAKIPLLRLDYVPEVPLHVTLEKL
                     SGDMDSLPPLLEQISVDLQQSADDIEQLGQDIGALSTQTTEIKSSLAEAGPILESYLK
                     ILSDLQVDTDSLFTSLPSYLNIACFALAAFALWAFLSQIMRLLDGLDMLKSGKIR"
     gene            15780..16706
                     /gene="argF"
                     /locus_tag="PKL60_00765"
     CDS             15780..16706
                     /gene="argF"
                     /locus_tag="PKL60_00765"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNW94696.1"
                     /translation="MHKDFLDIIDYSQEELQGLLDLAVRLKREYREGGNEPVLKGKVL
                     AMIFQKPSLRTRVSFDMAMRHLGGDALYLSPNEIGLGKRESVADIARVLAGYVHGIMA
                     RVFEHQHVLELAHWANVPVINGLSDFSHPCQALADALTIYENFGRLKGVNVTYVGDGN
                     NVAVSLMQISAKMGANFSIANPEGYDMPGAAVQTALEIARSSGSRLSFVRDPHEAVRF
                     ADVIYTDTWTSMGQEEEAAERERVFPPYQVNDQLVSEANPEVIVMHCLPAHRNHELTD
                     SIADGPHSRIFPQAHNRLHAQKAILYTLLRGE"
     gene            16708..17910
                     /gene="rocD"
                     /locus_tag="PKL60_00770"
     CDS             16708..17910
                     /gene="rocD"
                     /locus_tag="PKL60_00770"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNW94697.1"
                     /translation="MNSQDFINLEDQYGAHNYQPLDVVLTKGKGVWVEDVEGNRYLDC
                     LSAYSALNQGHCHPKIVQAMTDQLQRVSLTSRAFRNDKLGLFYKKLSDISGYEMSLPM
                     NSGAEAVETAIKVARKWGYAVKGIPKYEAEIIVAAGNFHGRTVTIVSFSTEPLYKDAF
                     GPFTPGFKVIPFGDLAALEAAIGPNTAAVMLEPIQGENGVIIPPEGYLRGAADLCKQN
                     NVLFIADEIQTGLGRTGKLFASDYEGVRPDMTIIGKALSGGLYPISAVLADKAILDLI
                     LPSEHGSTFGGSPLAAAVGIAALEVIEEEKLPERAFTLGKYFMDHLSEINSPNIKEIR
                     GKGLLIGVELKPEAGGARRFCESLQSEGILAKETHTHVIRFAPPLVIEQETLDWALPR
                     IARVLRQA"
     gene            17924..19366
                     /gene="pyk"
                     /locus_tag="PKL60_00775"
     CDS             17924..19366
                     /gene="pyk"
                     /locus_tag="PKL60_00775"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNW94698.1"
                     /translation="MNRFAKIVATLGPSSSDESTLRELIEAGMDVARLNFSHGTHEEH
                     AEKVKLLRKLSEELGKPVSILMDLQGPKLRIGKLEKDVVTLQSNQVVALSSSENPDHV
                     REGTIFIPFDVPRLHEALAPGNHILMDDGQLEFEVLELDGENIYARVILGGNLKSHKG
                     VNLPGSKLDIPCLTPKDLEDLQFGLDIGVDLVAISFVKQASDILTVRETMRRMSSLLR
                     LPPIVAKLERPEAIANLESIIDATDGVMVARGDLGVEMSPAVVPSAQKEIIRAANHKG
                     KFVITATQMLESMINNPRPTRAEAADVANAIFDGTDAVMLSAESAAGKYPVQSVRMME
                     SIIRESEKHLKEWGHLDSLVSTNQLDNAISIVRAAKDLTVNAKVSAIVVFTISGASAL
                     SMSKTKPSVPIYAFTPEIRTYQWLGIAWGVTPLLVPHADTLETMIRHVETAISSSTPL
                     RAGDQVVVISGFPVGEFGKPNLTLLYTLRG"
     gene            19742..20737
                     /locus_tag="PKL60_00780"
     CDS             19742..20737
                     /locus_tag="PKL60_00780"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNW94699.1"
                     /translation="MLKFDEDKIIKSINGALALRPKIETIADELYDRGFDAIFYLGIG
                     GTYASGIQVVDSVEGKSRLPIYLLHAARYAAHGNAHVTGQSVIVIASDSGTTSEMIEA
                     VKKAKQAGALIVGFVEFGDSPLARLSDRLISYPHNEQLKLFMFAHRLMNRNGEFEDYE
                     RFYRELEAHLADAYVDTSKRADAFALAFAEMHKDDPLHYYVSAGNHYGAAYSYAMCYV
                     EEQHWIRSKSIHAAEFFHGTLEVIDRDTNITVFVGEDEERPLAERVARFLPRICARHT
                     IIDTKDYPLEGISPEFRGLISYLVCHAVTERIDAHIEKLNCHPMEIRRYYRRLDY"
     gene            complement(21047..22090)
                     /locus_tag="PKL60_00785"
     CDS             complement(21047..22090)
                     /locus_tag="PKL60_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNW94700.1"
                     /translation="MDNTQTARTERNFVGLSPVDYRGKPSTLCPGCGHNTISNQIQQA
                     IYELGLMPEMVAKFSGIGCSSKSLNYFLQRSFGFNGLHGRMPTLVTGACFADRRIKAL
                     GTSGDGDTASIGLGHFKHIIRRNVPMVYIIENNGVYGLTKGQFSATSDLGLELKHQGK
                     NFLPPLDLCMEALISGAKFVARAFSGDIKQMKELLKAAVMHNGIAVIDVISPCVTFNN
                     RDEALQSYTWGRENEAPIQDITYVPRARTIEIKDFQEGETREVRLHDGSSILLKKIDR
                     DYDPTDKNAALDLLTESYKNHYFATGLIYIENAPTNIYDEFDFVEEPLNRLTPDRLRP
                     EPAALERINQLYQ"
     gene            complement(22083..23915)
                     /locus_tag="PKL60_00790"
     CDS             complement(22083..23915)
                     /locus_tag="PKL60_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNW94701.1"
                     /translation="MEHPTTNTQAESSASVNDFCMTLCTVNGSGSATANTILYRALFN
                     MGIPTSGKNIFPSNIKGLPTWFVIRVSERGYLGRVPYDDIIVAMNADTFQKDLSYLPE
                     GGVVFYADHLQKPADARTDVVYYPMPIRQFVRQAEVPRNLREYMENMAYVGVVGQVLG
                     IPLENIEYALTKHFGSRQNARESNLNMIKTGFAWAAANLTKQDRYSVRKLAPLTDCIL
                     TDGNNAAALGALFGGAQFCSWYPITPASSLVESMIAQNPKLRRDPQTGKNTCIILQVE
                     DELAAAGAAIGAGWAGLRAMTATSGPGLSLMTENIGLAYFTETPVVIWDVQRVGPSTG
                     LPTRTAQGDLAMVYRLGHGDINHILLIPGSVNECFEFGWKAFDIAEHYQTPVFVMSDL
                     DLGMNQWITDKFAYPDRPIDRGRILWEEDLARFEGSWGRYKDIEGDGIPWRTVMGNQN
                     PAAPYFTRGTGHDEYGNYSEDPQNWAEMIQRIKRKLQNSCEYLPEPILREKPGARLGI
                     IAMGSTDPAVVEGQDQLEQAGLPADYLRIRALPVCAAVREFLENHDRVYVLELNRDGQ
                     LYGILIQELQAFSQKLFSLAEVDGLPMTAAWVKQQIIVKEQQNG"
     gene            complement(23941..24618)
                     /locus_tag="PKL60_00795"
     CDS             complement(23941..24618)
                     /locus_tag="PKL60_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNW94702.1"
                     /translation="MKSKNELLDYLAEASRAQAPIPSIPQLSTLLGISVASVREQLEV
                     ARTLGFVDVKTRVGIQNREFSINQCLELCLRYGAKVQPDVSDALRDLRKHIEISYWHE
                     AAPQLTSRQIQTLHRIVDAAFAEISREPVRSPQPEHRDFHMLIFEHLENPLANSILET
                     FWNLNAEYERNLPVDRAYLENVWNYHRRIADALASRDYERGYQWLVEHMDLVKQRKKA
                     DLSQRFE"
     gene            24903..26672
                     /locus_tag="PKL60_00800"
     CDS             24903..26672
                     /locus_tag="PKL60_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW94703.1"
                     /translation="MVNLSMGVSEFSIPFRYKTNKANPVVWLLSHTLHHWAVFLAALL
                     GAVGNAALASIPAIQFGIVFTNLTSGAPDKNVFIQAALVITFSQVLRGLFQLMRNLAF
                     EVTAQRIERDVRDEFYISLLGKSMTFHSLQSVGDLMARATNDIREVNFLYSPGINMVF
                     GSINFLFVPPIIAWQIHPALLLTPLLFILTYFIAVTAYLKKLRPITDKVRSSFGLMNS
                     RLAEAIDGVETVKAAAQEENEIALFRRNIRAYRDASVEQGDIEARFLPLLLLALAMGF
                     GLLHALVLFQQGTIPLGDVIKYFSALALLNFPTNSSIHSYSQISLGVASARRLLSIMN
                     TENNLDKNDAGRAEPLRGEVRFTNLSFSYNTHNTALQNINFVVKPGQTVAIVGQTGSG
                     KTTLVRLINRTYDPSEGSVQIDGCDLRDWRLDTLRSQISIIEQDIFLFSTSIADNIAF
                     SKPGATRAEVEAAARKAQAHAFITALPEAYDTIIGERGVTLSGGQRQRIALARAFLTD
                     PKILILDDSTSAIDSQTEDEIQRAIKAASEGRTTFIITHRLSQIRWADLIIVMRRGTI
                     SDIGTHTELMQSSASYRNIFTES"
     gene            26678..28483
                     /locus_tag="PKL60_00805"
     CDS             26678..28483
                     /locus_tag="PKL60_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW94704.1"
                     /translation="MGFSAGLASESYDRQYSNKTLLGRIWAYAKPERKRILVILFAVL
                     LHGGVGALPPVIVAKVLDEGVAGIPDHQVFYLLAAVIIVLELLGYLLYYINHRLLARV
                     IADINRSLSTDAFAASMRQDMAFHDVFSSGKIVSRITTDTRDFSTLITLTMDVANNLV
                     QSVVTSIILLTAEWHLALLFFLTVPAFILFVSLYRNLARRVSRQGMRAMANVNATIKE
                     TISGIAIAKNFRQEERIYDEFKRSNILSCRTNVKRGMVLSTVFPITRMLGALATAMLV
                     YFGAISVTQGVITAGAWYLFILSTDRFLQPILSITSYWTQVQTGLSAAERILALIDAE
                     HTVIQTGGYNPENYRGRIDFNHLTFRYANGYTVLNDFSLHIREGENIAIVGHTGAGKT
                     SIARLIARFYEFQSGELLIDGKDIRSYDLHALRQHMGLVTQVPFLFEGTVEENIRFAR
                     PEITREEILALASQIGGGEWLDTFANGLDTRVGERGAQISMGQRQLVALMRVLAQNPS
                     VFILDEATASIDPFTEKQIQTALNLILNQSTSVLIAHRLSTVQSADRIIVLRDGDIIE
                     EGTHSQLLKQGGHYSELYNTYFRHQSLDYAEHAGG"
     regulatory      28560..28652
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            28734..29117
                     /gene="gcvH"
                     /locus_tag="PKL60_00810"
     CDS             28734..29117
                     /gene="gcvH"
                     /locus_tag="PKL60_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNW94705.1"
                     /translation="MNFPQELKYAQTDEWVKLENGIATVGISDYAQDALGDIVYFEFS
                     VEVGDHVQAGGQLGQVDSAKASSEVMLPVSGVVTEINEAILDEVEILNDDPYGKGWLL
                     RIKVDDPAELEKLLSAEAYRAYRVG"
     gene            29123..30478
                     /gene="gcvPA"
                     /locus_tag="PKL60_00815"
     CDS             29123..30478
                     /gene="gcvPA"
                     /locus_tag="PKL60_00815"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNW94706.1"
                     /translation="MFTPHTQKDVEVMLKTIGIGKVEDLFNEIPEKFRFPELKLPASL
                     TEMEAADELEGIADANGSTKTFLNFIGAGAYDHYVPAAVDMLLRRGEFYTAYTPYQPE
                     ISQGTLQAIFEYQSLICELTGMDVANASHYDGATAAAEAVILAYHHFKGKRTKFLISR
                     AVNPQYREVVRTYMRGSQEISIQGDDPETGIDASMEKLISRIDPNTALVMVQYPDFFG
                     RVIDYKPLIAAAHSAGALVAVAVNPTALGVLTPPGEIGADIVFGEGQPLGIPLSYGGP
                     YLGIFAVTNDLLRKISGRLVGETVDSAGRLAYVLTLTAREQHIRREKASSNICTNQGL
                     MALASTIYLSLLGKQGFEAVSNLCFQKAHYAAKQISSIQGYTQEFSSQPFFHEFVIRS
                     PQPVKHLLEDLRAYDILGGYDLGSDYPELEGCLLIAVTEKIGKEDIDLLHDALIEVTH
                     D"
     gene            30471..31925
                     /gene="gcvPB"
                     /locus_tag="PKL60_00820"
     CDS             30471..31925
                     /gene="gcvPB"
                     /locus_tag="PKL60_00820"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNW94707.1"
                     /translation="MTEPTIFELSSAGRQGVRFPASDVPEVELPAAFRRKELPLPELA
                     EIDTVRHFTRISQKNYAIDLGFYPLGSCTMKYNPKINEVTSRLPGFALTHPLQPEDTV
                     QGNLYLMYTLQEWLKEIVGFAGVSLQPAAGAHGELTGALIIQKYHADRGDSQRTRILI
                     PDSAHGTNPATSAMSGFEVVNLPSDARGNVDLEALKGLCDERLAGLMLTNPNTLGLFD
                     ENVTEVIEMVHAAGGLVYGDGANLNALLGIVRPGDLGFDIMHLNLHKTFSTPHGGGGP
                     GSGPVAVCQRLVEYLPDPVVAIVEPGSEELPPLYGYAHPAKSIGRVKSFNGHFGMLVR
                     AYTYIAMHGGNGLRNIAEKSVLNANYLLAKVKDAYHLAYDRRCMHEFVVEGHWADAPG
                     IRAIDIAKRLIDYNFHPPTNYFPLIVHEALMIEPTETESLETLDAFADAMLKIAAEAR
                     ENPELLHEAPHNSPVGRCDEVRAAKQLILRAPKV"
     gene            complement(32108..32869)
                     /locus_tag="PKL60_00825"
     CDS             complement(32108..32869)
                     /locus_tag="PKL60_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNW94708.1"
                     /translation="MKITTWNVNGYRAALSKQGFDWVMEHQPDVLCLQEIKVKPEQLS
                     GEQRELQGYKSFWNPADRPGYSGTAVFYKEPPLEIGTGLGIEAFDTEGRVTRLKYPDF
                     FLYNIYFPNGGEENQRVPFKLDFYARLLEVCEEHHEHGENVIITGDFNTAHNEIDLAR
                     PKENEKNTGFLPEERVWIDKFLEHGFIDVFRELYPLRAAYTWWSYRMNARVKNIGWRL
                     DYFLVSAPLMTRVRDVIILDEIMGSDHCPVTLELC"
     gene            33008..34348
                     /locus_tag="PKL60_00830"
     CDS             33008..34348
                     /locus_tag="PKL60_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNW94709.1"
                     /translation="MAPASVKAQSAQLPVYIVESGDTLYGIADQFYTTIDELLAVNNR
                     SIGDTLRPGDRLYIPGLEGLQGVLSTDYVPLGASLRSLSRRTQSDPASLVRLNKFTSQ
                     SELFVGRKIALTTSETTQDLQTMPSLLPGQSWMERSILAGQNPWALARLNRLGSPNAA
                     LPQDAYYAHSAQSNPNSLAIPGITSLVIGNLPLVQGGTFVLELTSEQPVSLSADLAGV
                     QPVFFTQDNGSQIAFGGINALQEPGAYPLTIEVTNADGATYRFDQWTIIGSGNFETDE
                     TLKVDPETVNGDNITAEDALFKQIVTVLTPIRQWSGVFQYPTKGGDCVNSRFGSRRSY
                     TGTDKIYYHTGLDIGWCYGIDVFAPAAGTVVAVLPEQIVRGNTLVIDHGLGVFSIYMH
                     LQDFLVAEGDQVQPGQLIAHIGNTGRSSGPHLHFEIDINGTPVNPVIWLNREFP"
     gene            complement(34376..34457)
                     /locus_tag="PKL60_00835"
     tRNA            complement(34376..34457)
                     /locus_tag="PKL60_00835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34421..34423),aa:Leu,seq:gag)
     gene            34520..35533
                     /gene="plsX"
                     /locus_tag="PKL60_00840"
     CDS             34520..35533
                     /gene="plsX"
                     /locus_tag="PKL60_00840"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNW94710.1"
                     /translation="MKTTIVLDAMGSDKRPQPEVEASVQCAETLGVDLILVGDEAVLK
                     PLLAARPGDKTHVRIAHAPEALEMSDHIAEARAKRNNTMQVGMDLVRSGAAQAFVTAG
                     NTGMAMYFATKTFGLIPGVIRPSLCTTFPVKNGKCVVLDIGANAECRPEFLVQFGRMG
                     SVYARLLLGRDQIRVGLLANGEEEGKGNELVKAAGPLMRAEVPGFVGNIEGKELFAGE
                     VDVAVTDGFTGNILLKSTEAVSRLILETLKEELLRSTRTKLGAALAKPAFNSVKQMID
                     PSEIGAAPLLGIDALVFVGHGRSDAKAVLSAVNQAKIAVESGLLDSLRQSIPSDNSGE
                     SHS"
     gene            35530..36249
                     /locus_tag="PKL60_00845"
     CDS             35530..36249
                     /locus_tag="PKL60_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94711.1"
                     /translation="MKLIINEKLIKKNKTIGQITTFASLAVLAIGLVFAFGKDMTKIL
                     YSYIALIVGFVLSQVGMYYSTRFGRSPRFDELFNDAFDKLRHEYSFYVYSSPVPYLLL
                     GPCVIWVPIPLNASGKISYENGKWKQQGGNFMMKIFGQEGIGRPVQEVSANTAALRNY
                     LTGKGIPEEEQPEIRHIMVVMMKNTIIGEVSQAPIPVVNLEDLKRSIRRVDREECEKP
                     LTEEQLARLTSALDEYSKKNS"
     gene            complement(36254..36997)
                     /locus_tag="PKL60_00850"
     CDS             complement(36254..36997)
                     /locus_tag="PKL60_00850"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNW94712.1"
                     /translation="MAEERVQKILAAAGLGSRRACETFIEAGRVMVNGKVIHLGDKAD
                     PARDSISLDGKLLRLKQDKIYIALNKPKGYLSDIDEDHPKPTVNDLVGLKEPLFAVGR
                     LDLDSEGLVLMTNDGDLANRLTHPRYKHEKEYEVFVIKQPDEEQLAIWQRGVVLEDGY
                     RTLPAKVEVMNNAGGGTWLRITMREGKKRQIRKTGARIGLPVAQIKRVRIGTLKLGSL
                     RSGEWRYLSPAEVKRLRQYAGLDKDEKNS"
     gene            complement(36997..37575)
                     /gene="scpB"
                     /locus_tag="PKL60_00855"
     CDS             complement(36997..37575)
                     /gene="scpB"
                     /locus_tag="PKL60_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNW94713.1"
                     /translation="MSTEVEISPETKTNLSRLEALLFAAPGLSPVEDLAKALEMSPAE
                     LEQLAATLEQHYEESHGIRLQKIRNQYQLVTAAEHAQLIEQFLGLEVSSRLSQPALEV
                     LAIIAYQLASTRPEIEAIRGVNSDTVVKSLLAKGLIEEKGRSEAPGRPILYAVAPDFL
                     LHFGLESLEQLPEMDIDALKAAQPQEQRILKD"
     gene            37651..38607
                     /locus_tag="PKL60_00860"
     CDS             37651..38607
                     /locus_tag="PKL60_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW94714.1"
                     /translation="MRVALTGAAGFLGSHLADRLIGEGHEVIGLDNFITGDEANIAHL
                     SKSGRFRLIRQDVSTFIDIPESLDFVLHFASPASPNPASPFGYPNLPIQTLKAGALGT
                     LNTLGLAKAHKARYLLASTSEVYGNPQVHPQTESYWGNCDPIGPRSVYDEAKRFAEAL
                     TMAYMRFHNLNTRIVRIFNTFGPRMRLDDGRVVPNLIQQALRGEPLTIYGSGSQTRSF
                     CYVSDLVEGIYRLMLTEEHEPVNLGNPNEISIREFALLINELTGNPAGLITRFAEGLG
                     DDPQRRRPDITRAKTLLGWEPQVDLKTGLHETIRYFQKKDRA"
     gene            38604..39092
                     /locus_tag="PKL60_00865"
     CDS             38604..39092
                     /locus_tag="PKL60_00865"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNW94715.1"
                     /translation="MTKKSTPEALRFGKFLMVGALNTLIDFGLMNLFSGWLRMPLMLA
                     QALSFLLAVINSYVFNRLWVYPETQEKSLAGQFSKFALINAAGLALRTLTIPPVDSWF
                     RQLISNNQIQLFGLSPQALSRNFALAVILPLTLILNYLANRYWTFGDIVIAQSGNKKA
                     QV"
     gene            39092..39688
                     /gene="rsmD"
                     /locus_tag="PKL60_00870"
     CDS             39092..39688
                     /gene="rsmD"
                     /locus_tag="PKL60_00870"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNW94716.1"
                     /translation="MGTLRVIGGSAKGLRLKTVPGDTTRPITDLVKEALFNILGDEIS
                     GSKVLDLFGGTGAVGIEALSRGAETALFLEKSREAVRVIQENLRTTRLGEKAQVLHQD
                     AFTFLAQAPASAFDLVYIAPPQYKGMWQKAMDLLDQQPGFLNRGGQIIVQINPVEWEE
                     RAYLHFTEFDSRKYGDTLLVFFEPNQAENDITNSSRAS"
     gene            complement(39646..40152)
                     /locus_tag="PKL60_00875"
     CDS             complement(39646..40152)
                     /locus_tag="PKL60_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNW94717.1"
                     /translation="MGIAAQGLSADRYKVIPRVLVFPFNVDGLVLLLHGAAHKRIWAG
                     LWNGIGGHVEAGESVLDAARRELLEESGLTAQSWTFCGQVLVDTREKTGIAFSVFRAE
                     MLAGELLESAEGSLRWFSLEELSQFPLVEDVPVLVGKAARTSAGGQPFWGVYRYDARE
                     ELVMSFSA"
     gene            complement(40218..41390)
                     /locus_tag="PKL60_00880"
     CDS             complement(40218..41390)
                     /locus_tag="PKL60_00880"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNW94718.1"
                     /translation="MAKKRIGVMTGGGDVPGLNLAIKSVVLNAVEKDYEVIGIRLGWG
                     GLLRYNLDNPESTADLIRPLDVNSVRKVDRTGGTFLHTSRTNPSNVRPKDTPEFLLNS
                     AYGKVVKPDGTKDFTDYILKVLDYHGLDSLITIGGDDTLSYSAPLYQEGFPVVAIPKT
                     MDNDVYGTEYCIGFSTSITQSVSLITNFRSSVGSHERIGIVELFGRNSGETSLVTGYL
                     SFADRTIICEVPFNFHKLADFLVADKKKNPSNYSMCVISEGAVSDGGVIVESGEEDAY
                     GHKKLGGIGELVGDQINALTGQHIMFQKLAYLVRSGPADMLDRMVALNYGTLAMQLTA
                     RKEFGKMVAINNGVYTTVPIDSSIQGKRHVDVDMYYDKENYRPHIQDIHNLPMFLV"
     gene            complement(41433..42659)
                     /locus_tag="PKL60_00885"
     CDS             complement(41433..42659)
                     /locus_tag="PKL60_00885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNW94719.1"
                     /translation="MLLLKKTAQYWVPLLVVALAAGLILLNLQLSEQFLYQDELLPRW
                     SAARGWMRDGTSPYSEQAYQEAQALYDQLGYTAPAFSQGRFIDLVWYVFLYIPFSFLP
                     YPIARAIWMTLTTAAIFFSAWFGIDLAGARMRSLERVLLALLITCSYPFFKLILSASI
                     LPLFIFVLLAACRSALAGKGTSAGVLLFLCVGMLPVSLLPAVFLIIWLNARKQTDALP
                     VFLVGLVFLALTSLILFPGWIPQWFTSLIAAQPRFDWLDTPLMRLAAFFPGAVVPLAA
                     ALHVLLLFAMLVEWYGLAENDERRFRWKLMLTLTLLSLFNPAGSPAFLILAWPGFLIF
                     YDFLQEKWKISGKIIFWTILLLLLYSNWQVFLRTQNWSSKEATWVITALPIAALVCLG
                     WTRWWAMSSPKPLRDA"
     gene            42889..44091
                     /locus_tag="PKL60_00890"
     CDS             42889..44091
                     /locus_tag="PKL60_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNW94720.1"
                     /translation="MARINNDNQVLDDDRESSALERLLDFGRKKTYVTYDDILQFFPD
                     AEQDLDQLEEAYTALLGAGINYTEETSAQDEPSETELSDVASEESALDDLANIDTDDT
                     IGLYLKEVSRVPLLHDIEEEQQLAQRIERGRAAREDLAKGNIGPKKRLELRALIEDGW
                     AAREHLITANSRLVISVAKKYMGRGVPFLDLIQEGNIGLIRATKKFEYQRGHKFSTYA
                     TWWIRQAVTRAIADQGRTIRVPVHMGDQINKLLRVQHQLTQKLGRDPSVEELAEALEV
                     PPKKVENMIQVARRPLSLETPTDDEEDSVLGDFIEDDEAPPPDDTATYNLLRAHLDEV
                     LDTLPPREVRILQLRYGLLDGQAYTLEEVGRKMGVTRERVRQIEAQALTRLRHPSVRR
                     KLRDYLGE"
     gene            44145..45098
                     /locus_tag="PKL60_00895"
     CDS             44145..45098
                     /locus_tag="PKL60_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94721.1"
                     /translation="MESVNPANKHTEPKATGSPQPLQISTPDPREATRPVRIAQSKPA
                     NLGVSAQPMAATAPELENTRPVQIRIVPAQTPPTATPGDELPAWLISFAAQETKPAAP
                     ANPEEDTRPIPLQLPDEAPEPEAAVIPSHASGLQAPEADNGWTVEIIPEPGAQEINLD
                     TAAQPDAGEIGIESRPETAAAEQEDLPGEVGAAMAEDIIPQPSGSGGLAAETSAPPGN
                     PALENSQAFSLEKALDESRIEEAGAMLMHMKSDPAARACALQVLRSRLDLRAESLPLW
                     QYYAELCSADNQPGLARQALETAEKLKIASGEEHGTIAGIG"
     gene            45073..45522
                     /gene="ndk"
                     /locus_tag="PKL60_00900"
     CDS             45073..45522
                     /gene="ndk"
                     /locus_tag="PKL60_00900"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNW94722.1"
                     /translation="MERSLVLVKPDGVQRGLIGKVITALEDRGLRLTAARFLQVNREL
                     AEAHYAVHAGKPFYDALITFITSSPVMAMVWEGPDAIAAIRQTMGKTRGTEAAPGSIR
                     HDYALQARYNITHASDSPENAEAEIALWFRPEELVRWSRELEKWIYE"
     gene            45519..>45746
                     /locus_tag="PKL60_00905"
     CDS             45519..>45746
                     /locus_tag="PKL60_00905"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNW94723.1"
                     /translation="MTEHGLSDLVVVYRAKGLVDGEIIRGFLAAHDIPASVSQEAAGK
                     IYGLTIGDLGVAEVLVRAQDEQNALELLREME"
BASE COUNT        10272 a        13504 c        12276 g         9694 t
ORIGIN      
        1 ggcggcattt atcctgtcaa cccgtcggca gttgaaatcc tcggtttcaa atgctacccc
       61 tctgtgacgg acatccccta tgtcgtggat gccgcggtta tcaccgtgcc tgctaaatcc
      121 gttttggacg ctaccaaact atgcggcgaa aaaggcgtca agggcttaat cattattact
      181 tccggttttg ctgaagttgg cgagattgaa ctggaacatg aactggtgcg ggtcgccaaa
      241 tcttacggca tgcgcatcct cggtccgaac atcgtcggca cgctttccaa cagcgataag
      301 atgaacgcct ccttcgcgcc cttcctgccc ttgccaggca aagcttcgct tgtttcccaa
      361 agcggcgctt tgctcattgc catggacgca ggtacctaca cccgccgtgt cggctttgac
      421 aagatggtgt ccatcggcaa catgtcggat gtcaatttct ccgagatggt cagctacctc
      481 agcgaagacc ccgataccaa ctgtatcgcg ctctacgttg aaggattgcg cgacggcccc
      541 aagttcatcg aagccgcccg caattgcaag aagccaattg tcgtgctcaa gtccggcgtt
      601 tccgcgcacg gcgcggcagc tgccgcctcc cacaccggct ccctggccgg cgcggcgaag
      661 atttacggta ccgccttcaa gcaggctggc gtgacccagg ctacagacct gaacgacctc
      721 ttcgaccgca ccctggtact ctccctgcag ccgcgcatga agggcgataa cctgctcatc
      781 ctgaccaacg gcggcggcgt aggcgtgctc gccacggatt ccgctgaaaa gtacggcctc
      841 ccgctgcatt tcgcccctgc ggacgtgcag gaagaaatga agaagcacat gcccgattat
      901 ggctccgcca aaaacccggt ggacatgacc ggtatggctg gcaatgactg gttctacgag
      961 accacccgca gcgcctactc ccatccctgg gtggacggcc tggtggtcct gtattgcgaa
     1021 accgccatca cactcccgga agaaatcgcg gatgcaatct accgcggcat caaagactcc
     1081 ggcgtcacgg acaaacccgt cacggtttcc tttgttggcg gcgaacgcag cgacaaagcc
     1141 atggaatggc tggtggaacg cggcatcccc gcctacaacg caccggatgt ggcagtcaaa
     1201 gctatcgcca ccctgcgcga cgaccagcgc atgcaggaaa tgaaccacaa cggcggctac
     1261 gaacccgggg acatcaactc cgctttcgcg cgcgaaatta tcgccggcgc ccgcgccaaa
     1321 ggccgcgacg cgctgaccga agtggaagct aaacaagttt tcaggctcta tggactgccc
     1381 gttaccacta ccgaactcgc cagctccgaa gaagaagcgg tcaaattggc ggaaaaagtc
     1441 gggtatccgg tcgtgatgaa gattgtctcc ccagacattc tgcataagtc cgatgcgggc
     1501 ggtgtaaaag taaacatcaa gaacgccgag caggcaaaag aagcttaccg gaccattctc
     1561 gagaacgccc ataactacaa agccgacgcg tttatcgacg gcatcgccgt gcaggaaatg
     1621 gctccctggg caaccgaggt cattatcggc tcggtcaatg acaacacctt tggtccgaca
     1681 gtcatgttcg gtctgggcgg catcttcgtc gaagtgctca aggacgtaac cttccgcgtg
     1741 gcccctattt ccctggaaga agctttgggc atggaagatg aaatccgcag cgccgcaatc
     1801 ctgaatggaa cccgcggtga aagcgcccgt gacaaggatg ctcttgccag ggttctggca
     1861 gcctacgcct atatggtcta tgacctgaag gatgaaatca aagaatcgga tgccaacccc
     1921 gtcatcgtct acgaaaacgg gcagggcgta aaggttgtgg atgcgcgcat catcctgacc
     1981 acgaaatagt ttgttctaca gctttagcat aagcaagccg ctgctggtac aatcaacagc
     2041 ggcttgttct tttcaagccg gcattttttg aggtgttaaa tgaaagaaaa aaagaattac
     2101 ttgattgaca tggacggcgt gcttgtgcac ggcgcgaaca tgatccccgg cgcggacggc
     2161 ttcatcaaca gacttatcga agaaaaacgt aagtttctca tcctgacgaa caatccgctt
     2221 tacaccaccc gcgacctggc acacaggctg caggttaccg gattaaatat cccggaggag
     2281 cgcctgtata cctccgccct cgccacggcg gcgtttctaa acaaccaacg ccccaagggc
     2341 actgcgtatg tgattggtga gcttggtctt agcgaagctg tccatgcgat tggctatacc
     2401 caaaccaccg ttaatcccga ttatgtcgtc ctgggcgaaa ccttcgcata cagcatcgaa
     2461 caaatcacga ttgccgtgcg cctgattacc gccggcgccc gtttcatcgc taccaacccc
     2521 gacccggtcg ggaactccga gcaaggtctg gtacctgcct gcggcgccat ggccgcgctg
     2581 attgagcgcg ccacgggcat cgcgcccttt tacgtcggca aacccaaccc gctgatgatg
     2641 cgctccgcac tgaactacct ggacgtgcac tccgaaaact cgattatggt cggcgaccgg
     2701 atggacacag acgtcctgag cggcatatcg agcgggatgg ataccgtgct cgtgctcagc
     2761 ggaaccaccg accgcgccag tatggagaaa ttctcctacc gaccaaaatg ggtattcaat
     2821 tcggtcgcgg atattaatcc gaaggacctt gactaagctg caccctgtga gatggaacct
     2881 aagcttaccc gcagagaatt cctgaaactt ggcctgattg ggctcggcgc ggcggcggct
     2941 ctgcctgccc gcaaagtctt tgccgccgag aaagccccac gccaggtgct ggttggcaac
     3001 cagaacggcg tcagtgtgca caaactaccg gatgaagaca gcctgattat ttaccagatc
     3061 ggctacaacg agattgtgaa cgtctacgac gaggttgaaa gcccggccgg acccttctgg
     3121 aacccgatct ggtaccgggt ttggggcggt tacatcttta gcggggatgt atacgaggtg
     3181 cactataaac tcaacagcct gaacacggat atccgtccga ccgggcagct tgctgaaatc
     3241 acagtgcctt atacgcgcgc gatgttctac agccggcttg agggttggat tccggaatac
     3301 cgcctgtact acggcaccac gcattgggtc atggacattg tcacgggacc ggacgaaaaa
     3361 ccctggtata aaatcaagga cgaaaagaac agcgtcatgc tggcggtgcc gtccgagcac
     3421 ctgcgttttg tgccggatga agagctgcag cccatctctc cggaggtgcc gctcgggaaa
     3481 aagcgcatcg agatttcgat tatgttccag cggctgaagg cgtacgaata cgacaacctg
     3541 gtgttcgaga ccaaaatttc ttccggtggc ctgaccccca tcggcaccta caatatccaa
     3601 accaagatgc cgtccaaaca catggggaat ggatatgtca ccagcgatat ccgcgcgtac
     3661 gaactggtag gggtgccgtg gaactgcttc tttgaaatga cagaaggcat tgccacgcac
     3721 gggacttact ggcacacgaa ttttggcacg ccgatgagcg ccggctgcat caacatgtcc
     3781 atggcagacg cgaaatggat ttacttgtgg accacgcccg tagccgcccc ggaagactgg
     3841 caaaagcacg gcttcggtac gcccatcacg cttaccaaag attagtcgac gaactccata
     3901 aaagcctggt tcccgaccag caccccgcgg cgcgtcaggc gcaagtgcgg gccgtccggg
     3961 aattggacaa attccaccaa gcgcttttcc tgcaagcgca tcatttctgc ggaaaaaagc
     4021 tcgcgtgcat cctgtccaaa cctgcgctga aatgccgaca gcgcaacccc ttcgcgcgtc
     4081 agacgcaagc cgagcagcat gctttcctgc atctgcgtgt acggctgtat tttcgaacgg
     4141 tttaggttgg ctggtgagaa gggaaactcc aggtttgcct ccccctgctg tatgcgcgtg
     4201 atgtaatcct gaataccggg cacattttct gtgcggaagc cgccggcaaa gctgtgagcg
     4261 gcggcgccaa ttcccagata cggcgtcgtc ttccagtagg ctttgttgtg gcgcgattca
     4321 caaccctctg ccaaagccca gttcgaaatc tcgtattgag tgtaccctgc ttgcgcaaga
     4381 aactcctgcg tcaattcgta catatcggcg gcaaggtcct cgtcaggttc gggcagcctt
     4441 ccgctcgcga tttgtttttc cagcggggtg ccctcctcta cgatcaggct gtaaacggag
     4501 agatgttccg gcttatatgt aactgcggtt ttcaggtttt cctgccactc ctccagcgtc
     4561 tgccgcggga gacctgagat gagatccaga ttgagctgct cgaaaccggc ttcgcgcgcc
     4621 cagcggatgc tttcttccgc ttgctcgcgc gaatggattc tgcccagcag cgccagctcg
     4681 gaatccgaaa aagactggac gccgaggctc aggcggttaa tgcccgcggc gcgcagttct
     4741 ctcaattttt tggggtctaa agtgccgggg ttggcttcca gactgatttc agcggaaggg
     4801 gcgaggccaa acgcgcgcgc ggtttcctcc agcagcccgc ctgccagccg ggattccagc
     4861 agggaaggcg tgccgccgcc aaagtacaca gtatccgctg attgccgctc gctgccggca
     4921 gccaaaccga gctgctggat ttcgttggca gccgtgttgg cataagcggg caggaattcg
     4981 agctgcccgg cgtacgtgat aaaatcacag tatagacatc tgcgtacgca aaacggcacg
     5041 tggatataga gactaaacat gcaactattt tatcagccca accctcgtga attgcggttt
     5101 tttcgagggc tgtatagttc aatgactatg agttggattg ctcgcacaca aagctccctt
     5161 acttccttat cgcgaaaaaa ctccccggtg cgagcctggg aagccattgc ccttccagcg
     5221 cacagggaga ctttcaagaa caaaagcgat gccgctgcca atgcaagcgc tgccaacatc
     5281 actgaatgat tcccgagagc ccttccacgc ctgacttgcg ggttttttga gggctgtgta
     5341 tactaacctt tctggaaaca actatgagtc aagaaaaaac tacccaaaac acgaaaattt
     5401 gcccggtttg tggaacccgc ttgagtgaga ccgcctcccg ctgcctggtt tgcggaaccc
     5461 agctttccac cactgctgca gtgaaaaaag gctcgggaat tccatcccga aagctgcctg
     5521 aggtccgcct gaacctgccc gtcgccattg cgctcgggtt cgtcttcctg gcgttggcag
     5581 gtctgctggt cttcttcatc gtcaaaggca gcccggccgc gccgcttact ccgggggaag
     5641 tcgtcgaagc aaccccgacc ctgacgccca cccagacgat tacccccact cagacgctga
     5701 cgcccacccc gctcccgacg tggacaccgc tgccgcccat ggaatacact gtccgcagca
     5761 cggattactg ctcctccatt gcggcagttt ttggcgtatc catccaaagc atcatccgcg
     5821 agaacaacct ggacgcgaac tgcactatca aagagggtga tgtgctccgt atcccgcagc
     5881 cgacgctcac gccctcaccc caaccgaccg ccacctcatc cggtatcgaa tctaccgaat
     5941 ccgactgcca gacgatcacc atcaaggtgg aatccggttg gacgttgagc agcatcgcct
     6001 tgaattacaa cgtaacgatg gcttccatcc gcgaatacaa ccgtatgacc aacgacgtgg
     6061 tctatgaagg cttggatctg ataatcccgc tgtgcgagcg caaacccacc gccggaccca
     6121 cccccacccc aacgcctgct cccccatatc ctgcgccaaa cctgctgctc cccagctcag
     6181 gcgccgcctt cagcgcatcg caggacgcta tcactttgca gtgggctgcc gtggccgacc
     6241 tgcgtgaaaa cgagctgtat cgcgtaacgg tggaagacct gacggctggc aacggacgca
     6301 tcctcgtgga gtacgtgcgg gatacgcgtt tcattctgcc caccagcttc aggccattgg
     6361 atagcacccc ccacatcatt cagtggtccg tggccgtcgc gcggcagatt aacagcggta
     6421 cgaccaatcc gatttacgag gaagcgggct tcctcagcga gaagcgcgta ttctcctgga
     6481 ttggtaccgg agtgagtgtt acgcccaccc cttgacggat cactgacatt cgagattttc
     6541 caaataacgc gttgccgaaa tggcagcagc agcgcccata ccggcagaag taatcacctg
     6601 ccggtaatgg ggatccgcga tttcaccagc agcgaaaacg cctgggatgt tagtcgtcat
     6661 tttgtcgtcg attttcacat agccatgttc atccaggttg acttgcccat caatcaggtc
     6721 tgtgttcggc ttgttcccga taaaaataaa gacagcttcc gatgggattt cacgtttttg
     6781 gccggttatc acgtcgcgca ccaacaggct gtgcactttt tcatcaccgc ggatttcgtc
     6841 cacaatagta ttccacagaa agtgaatttt tgggttggaa cgcgcgcgat tttccaggat
     6901 ggctccagcc cggagcgcgt ccctgcggtg aatcacggta acgctgcggg caaagcgcgt
     6961 cagaaagagc gcttcctcca gcgcggtatc cccgccgcca atcacgtgaa tctccttatt
     7021 gcgggtaaag aagccgtcgc aggtgccgca gtaagatacg cctctgccgg taaattcagc
     7081 ctcaccgggg acgagcagtt tcttcggctc ggcacccatg gccagaatca cagaatccgc
     7141 agtgtagctg ctgctgtagg tctgaatctg gaacggacgg ctctgaagat tgagcgagag
     7201 gacctgatca tactcgactt ttgccccaaa gtttaaggct tgctgttcga agaacttgcc
     7261 taattccacg ccttgaattc cctctggaaa accggggtaa ttctcaataa tgtcggtctg
     7321 agaagcctgc ccatacagca tcattccggt aaaaataagt ggatgaaaat ccgctcgggc
     7381 gccatacagc gcggcagtaa aacccgccgg acctccaccg atgattatga gttggtgatg
     7441 cgtatttgct tccattctta tcaggtttac tccattccag cctgaatttc atgatccgcg
     7501 atgcttagcg cggcgtctac aatgtcaaag ccctcgcgca gctgtacgtc gctgataatc
     7561 aagggcggta tgaacaaaag cgtttgaaag gtattgtaca tgaacacgcc gtgctcgagg
     7621 atataactct ttacgtgctt catcaccggg ctgaggggtt tggcggtggg gctaagcggc
     7681 tccttgctgg cgcggtcccg aaccagctcc gccgcgccaa acaagccgat agagcgtacg
     7741 tcgccgacgc aaggatgttt ttgtttcaaa tctgcgagca gcgcttttaa ggtttctcct
     7801 cgccgcaggg catttttcag cagcccttcc tgttcgatta cctcaatgtt ggcaagggct
     7861 gctgccagcg aaaccggatg tccgttgtag gtcattccgc ccacaaactg cctgtcgtca
     7921 taggtcgcgg cgatttcctc tttgattgcc actgcgccca acggcgcgta acccgaggtc
     7981 aggcctttcg ccatcgtgat gatgtcgggt accacgtccc aatgctggct ggcaaaccac
     8041 ttgccggtgc ggccaaagcc gcacatcacc tcgtcggtga tgagcaaaat ccggtatttg
     8101 tcgcacaaag cccgcacgcc ctgcaggtag ccgctgggcg gaacgagaat gccattggta
     8161 cccgttacgg tctcaatcag caccgcggca atggtatcgg gaccttcaaa gcagataatt
     8221 tcttcaaggt ggtcgacata agcgcggctg aagctctcct cgtcaacgcc tccatccggg
     8281 aagaaaggcg agcggtacgc gtaaggatcc aggaagtgca ccacgccggg catcgtttcc
     8341 ggttcccaat gcaggcggcg gtagtctcca gtcaaagcca tcgcgcccat ggtggctccg
     8401 tggtacgagc ggtagcgggt caagattttg aacttgccgg tgtaatcccg cgctattttt
     8461 accgcgttct cattggcatc cgagccgccc aaggtgtaga gaaagcgggt caggccaggc
     8521 ggtgtgaggg aagccagctt ttcacccagc agcgcccgcg ctttggtggc catatgcggc
     8581 ccggcgaaag tgagctcgcg tatctgcctg atcatcgctt cttgcacatg cctgttgccg
     8641 tgcccgatat tgacgcacat caccatggaa ttcaggtcca ggtagcgttt gccgcccgtg
     8701 tcccaaaagt agacgccctc cgccctttct acgggcagaa tttccgcctt acgctgcgct
     8761 gtccaggtcc ataagttatg tcggatggat ttggaaagaa tttcgtcact tttcatcttt
     8821 gcattcactc caaaacaagc cgttgtgcag tcaatgctaa cacaattcag cgtcaaatcg
     8881 catataatca acggaagttt aaaaggaaaa cgaatgacac acatagaaaa ttccctaaag
     8941 ttttttacag aacataccga caactttctc caacaatacc aggacctggt ccggattcca
     9001 tcggtttcca cggatccgga gcacaaagcg gacatgcagg cagcctgtaa gttcctggcg
     9061 gacaaactgc gcggactggg cgccgggcat gtagaggtct tcccgaccaa agtgcaccca
     9121 atcctcttcg cggaaatgcg cgcggatgcc gaagacgccc ccaccatcct gatttatggg
     9181 cattacgacg tacagccagc cgcgcccatc gaggaatggc agacaccgcc atttgagccg
     9241 accatttcgg gcgaatatct gaccgcccgc ggcgcgtcgg atatgaaagg ccaggtgatg
     9301 gccgccttgg cggcggttga aagcgtgctt gccgccggtc cgctgccggt gaatctgaaa
     9361 ttcctgctgg aaggcgaaga ggaaatcggt tccccctcac tggacggctt tatagcctcg
     9421 cacgccgatt tgctggcctg tgatatcgtc ctgaatcctg attccggcat ggtttccagg
     9481 gattccccga ccattgttta cggactgcgc ggactgctct acttcgagat tcgtgtggac
     9541 ggacctaagg cggacctgca ttccggtctt tttggcggga atgtggccaa ccccgcgaac
     9601 gtattggctg ccatcatcgc caggctgcac aaccccgacg gttccgtggc agtgcccggc
     9661 ttttacgata aagttttgcc gctcagcccg gaagaaaaac agcgcatcgc ttcagtaccc
     9721 tttgaccccc aagccctgct gcaaattacc ggcgtcccgc agctctttgg cgaggccggc
     9781 tattccccgc tggaacgcac cggcgcccga ccgaccctgg acgtcaacgg catttattcc
     9841 ggctttatcg gcgaaggcag taaaacgatt atccctgcct acgccaaagc caagatttcc
     9901 tgccggcttg tgccggacca gaaccccgat gaaatctacg cactcattca agcctacatc
     9961 actagcctga cgcctgaggc ggtacggctg accatcttca aacattctgg cgcgcctgcc
    10021 tatctggtgg acgatgcgcc cgggcttgag cagctctcca aagcgctcgc ggaaacctgg
    10081 aagaaaccgc tcagcttcaa acgcgaaggc ggcagcatcc cggtcgccac cacgatgcag
    10141 acccacctgg gggtcaagtc cctcctgacc ggcttcgggc tgcccgacga ccagatacac
    10201 tcccccaacg aacaccagca cctgccaacc tggaaattag gcgttcaagc gctcattcgt
    10261 tttttgctga gtttcgaaca tgccaactga agccgctcct gaactgaaaa tgccctccta
    10321 cggcggacag gccgtcatcg aaggcgtgat gatgcgcggc aagaaagctg tcgccatggc
    10381 ggtgcgctcc ccgcaaggcg gaattgtgat gttcgacgag cagctccccg cgctttaccg
    10441 ctcgatttgg ttcaaaactc ccttcgtgcg cggagttctc agtctgtggg actctctgga
    10501 actgggcatg cgctacctga ccaaggctgc taacgtgcag accggggaag acgagaaaat
    10561 tgagggcgca gcgctctttt tcactgtgct tgcagcggtt ttggtaggca ttgggctgtt
    10621 tttcctcctg cccgccctgg ctgccggttg ggtcgataaa ttcctgaacc ttggttcctg
    10681 gtggagcaac ctctttgagg gcattttgcg cctggcaatc ctggtccttt acatggcgct
    10741 ggtgggtaga atgccggata taaagcgcac ctttatgtat cacggcgctg aacataaaac
    10801 catcaacgcc ttcgaagccg gcgtggaact caccccgcag aacgtcgcac tgcagaccaa
    10861 agtgcacccg cgctgcggca cgtccttcat cctcaccctg gtcgtctttt ccgtgctgat
    10921 tttttccatt ctgggtccgc tgcccctcca ttggcggctc atctcccggg tactgctgct
    10981 gccggtggta gccgggcttg cctacgaata tatccgctgg gctgcccacg cgatggacaa
    11041 gagcgccttc gtgcgcgcgt tgattaagcc caacctgctt ctgcagaaat taacgaccaa
    11101 agagccgacc gaagacatgc tggaagttgc catcgcggct ttcaagcgca tgtacgcgct
    11161 ggagcaggac accgaagagg cgcagccgct gacttcatga aaacctggca gtctgtcttc
    11221 ctcgggttca ttggcgggct gctgctttcc ggcctgattc tcctgctcat tctcccgcag
    11281 cggggcgaac cgattcagct tgccaccgcg gcgcctgacc agcgcgcccg gagtacgccg
    11341 accgccgccg aaattgaggt atacgttgcc ggcgcggtgg caaacccagg cgtatacatg
    11401 ctcccggcag gttcgcgcgt gcacgatgcg ctttccacgg cgggcggcgc tgcccaggat
    11461 gcggattttg agcgcatcaa cctttctgcc ttcctgcagg acgggcagcg ggtgtatatg
    11521 ccgcgcgtcg gcgaagctgt gccagccgac gacacgacgc gtgggcagac tggcctggaa
    11581 gcggcatcaa acctggtaaa tatcaacggc gccgacctgg aaacgctaat gtccctgccg
    11641 ggcatcggcg aaaccaaggc ggccgcaatc ctgagctacc gcagcgagca cggtcccttc
    11701 acgagcctgg aagaactgct aaacgttccc ggcatcgggc aggcgcttct ggaggaaatc
    11761 cgtcccttaa ttatgctcgg accctgagta tcctcaacaa gcgcccttag cggaggtcaa
    11821 ggcaggtata atactccgca tggacaacaa tcaaaaacta aaagccgatt tacagaaagc
    11881 catcaaggat caggatccgc tgcgcaagcg cgttttacgc ctgctgattt ccaccatcca
    11941 attggcagaa gtcgccaaag gttccgcgct cagcgattcg gaatttgtcg cggtagtcca
    12001 aaaggaaatc aaaaccaaac tggatacgat tgcggacgcg gaaaaagcag ggcgcgcgga
    12061 tatggtttcc gaatcccggc tggaaatcaa ggagctggaa gcctacctcc ccaggcagct
    12121 cacggaagcg gaattggttg ctctggcaga ggaaatcatc cgggaaactg gcgctgcctc
    12181 cccaaaggat atgggtctgg tgctgaaagt cctcatcccc aggctggccg ggcgcgcctc
    12241 caatcaggat gccagtcggg tggttaaaga ctgcctcagc aaacctgctt gaccatggca
    12301 aaaaatcggg aaaacggcac aaaaccggca gggtttaccc agggcgcgct gcgccttctc
    12361 attctgcttg tcagcagtct gcttatattt gcttcgctgg ttttgcccga actgattacc
    12421 cgctccaaca tcccggcagg cgtgggcgaa gcagcatcgc aggaaatcct cgcgccgtat
    12481 tccatcacgt atgagagtaa ggtactgaca gaacgcgccc gcacgcaagc tgcggcggct
    12541 gttccgccgg tatttttgcc tgctgacccc agcatttccc gccatcaatt ggaaacgctc
    12601 gacagcgtgc tgtattatat ttccgcggtt cgcaaagaca acttttccac cccggagcaa
    12661 aaactgcagg acctcgcggc gatttcttcc gtgaaattga gcagcgcggt ggcagcccaa
    12721 ctgctcgaat tggatgagca ggactggcag gaaattgagg tggaagcccg ccgtgtgctc
    12781 gagcaagtca tgcgcgacac aatccgcagt gataacctct cgcaaattcg cagtaatctg
    12841 cccgctgtaa tcgacttttc gttccgcccg gaagatgccc agctcgtaat cgcgctggtc
    12901 tcccctctga tcacacccac cagcctgttc agcgaggaac agactaacgc agcccgcgag
    12961 caagcccggg caggcatcga accgatttcc cgccagatta tcgccgggga agttatcgtc
    13021 cggcgcggtc aaattatccg cgaggaagat tacgaagccc tgaacgtctt tggcctcgcc
    13081 aaacccgtca atcagacaga ccaggttatc accagcgcgg tcctgacagg cgttatcgcg
    13141 gtgttggcag cgctctactt ccagcgccgg caggaccaac cgttcttctc cgtcaaagcg
    13201 ctgttggtca tcgctggttt gtttctcatt ttcctcgcgc tggggcgctt cctggtggtc
    13261 gaccgtacga ttgtgccgta tctctatccg gtggcagctt tcggtctgac gctctcgatt
    13321 attttcaacc ttgagcttgg catcgtgatg agcatgttcc tcgccattct gatggtgttt
    13381 gacaacaccc gcgaagcgga attaggtgtt ttctatattc ttccggctgt cgtcgggatg
    13441 ctgacaatag gccgcgcgcg ccgaatcagc gcatttctgg tagccgggct gctcaccggc
    13501 atcgcgggga tggctgtggt ggtggctttc cgcctgggcg atacctatac cgatttgcta
    13561 ggcctcacct ccctgattgc atctgcgctg atttacgggc tcgcttcggg cagtctggcg
    13621 ctcctgctcc aatatgtggt gtcgcaaatc ctggatatcc ccaccgcgct gcagctgctg
    13681 gaagtttccc gcccggacca tcctctgctg cagtacatcc tgcgcaacgc tcccggttct
    13741 taccaacaca gcctgctggt ctccaatctt gcggaacagg ccgccgaagc cgtcggcgca
    13801 gaccgcatgc tggtgcgcgt tggcgcgctt tttcacgacg ccggcaaagc caacaatccg
    13861 gctttcttca tcgaaaatca ggtgaaggac aaactggatt cccacgataa cctggacccg
    13921 gctacagccg ccgcgaccgt catccagcat gtgcacgacg gcgtggagct ggcaaaaaaa
    13981 tatcacctgc cctcgcgcgt gattgatttc atccgcgaac accacggcac aatgcttacc
    14041 ctttaccaat actcccaggc ggcagccgcg gcggacgacc cggagagcgt cgataaagcc
    14101 ctctttacct accccggacc taaacctcag tcccgtgaga ccgccatcgt gatgctctcg
    14161 gacggcactg aagcccgttc ccgcgcggaa acgccgcgga gcgatgatga aatccgcgcc
    14221 ctcatccgcc agactatcga catgtacaaa aacgaaggcc agctggacgc gaccgacctg
    14281 acgctaaaag acctcagcac catcgaagat tcttttttcc ggaccctgca gcggtcttac
    14341 catccgcgca ttcaataccc cactctgaaa cccaacggaa gcgccgtgtc caagggcagc
    14401 gattccgccg gctaaacccc accctggaag gagcgcccaa tgatcgaatt tatgatcccc
    14461 cgcaaatatc gcactgtaat ctctgctgac cggctcctgc tcgcttacca acggacggtg
    14521 gaaaagcttg gcaggcgtgc tgagggcgca gtcaccctga aaatcacgga caacgccggc
    14581 atccgcaagc ttaataaagc ctaccgcggc ctggacgaag ccaccgacgt cctttccttc
    14641 gaaaacgaat ttgtcgacct ggaaacgggc aggcgtttcc ttggtgatat cgccatctcc
    14701 atcgagaagg ccctggctca ggcagaagaa agaggcttca gcctgcagga agagctggaa
    14761 atgcttttcg tgcacgccgt tctgcatctg aatggatatg accacctgga ggaagcggaa
    14821 ctggaagaaa tgaacagcct gcaggacgaa atcctgcagg aaattggcaa cccaatccgc
    14881 gggagtatca gccatgaatg agaccactct gaaaccgaag cattcccgcg gcctgatagt
    14941 ggtcttacta agcgtcatcg gcatgctcct ggcgcttttg ctggcggtcg gcgccctggc
    15001 ttcgcggtta accctcatcg atttttctgc caaactcggc ctgtcgattc aagaaacgct
    15061 gaccgccaca aacagcggcc tgaaactcat cagccagtcc ctggatgatg tggaagccag
    15121 ctttgacgaa ctgcagaatt ccatgaaaac gctggaaagc tctgtggcga atctatcccc
    15181 catgctgacg gacgtcagcg agctgattgg gacggatgtg tccgcgattg cctcggaagg
    15241 ggctaccacg ctgaaatccg cctcggaaag ctcgaaactt atcgattcca ccctgcaatt
    15301 tctggcaaaa attccactgc tgcgcctgga ttacgtcccg gaagtccccc tgcatgtcac
    15361 attggagaag ctgtccggcg acatggacag cctcccaccg ctgctggagc aaatcagcgt
    15421 cgatttgcag caatccgccg acgatattga acagctaggt caggatatcg gcgcactttc
    15481 cactcaaacc acggaaatca aatccagcct ggcggaggcc ggtcccattc tggagagtta
    15541 tctgaaaatc ctctcggacc tgcaagtgga tacagactcg ctcttcacca gcctgccatc
    15601 atacctgaat atcgcctgct tcgcgctggc cgcttttgcg ttgtgggcat tcctcagcca
    15661 gattatgcgc ttgttggacg gcttggatat gcttaagtct ggtaaaatcc gatgagattg
    15721 atgaggcagc ccagccggag cctggtctgc aaataacaat aaagaattaa ggagagtgta
    15781 tgcacaaaga ttttttggac atcatcgatt acagtcagga ggagctgcaa ggactgttgg
    15841 accttgccgt acggctcaaa cgggaatacc gggaaggcgg taatgaaccc gttctcaagg
    15901 ggaaagtact ggcgatgatt tttcaaaagc cttcattgcg caccagggtt agttttgata
    15961 tggctatgcg ccacctggga ggggatgctt tatacctctc acccaatgaa attggtctcg
    16021 gaaaacgcga gtccgtggcg gatatcgcgc gcgttctggc tggttacgtt cacggcatca
    16081 tggcgcgggt tttcgaacac cagcacgtgc tcgaacttgc gcactgggca aacgttcccg
    16141 tcatcaatgg gctgagcgac ttcagccatc cctgccaggc gctggccgac gcactgacta
    16201 tctacgagaa tttcggcagg ctcaaaggcg tcaacgtgac ctacgtgggt gacggcaaca
    16261 atgtagccgt atcgctcatg caaatcagcg ccaagatggg tgcgaacttc agcattgcca
    16321 accccgaggg ttatgacatg cccggcgcgg cagtccaaac cgcactggaa attgcccgca
    16381 gcagcggctc ccgcctcagt ttcgtgcggg acccccacga agcggtgcgc tttgctgatg
    16441 tcatctacac cgatacctgg accagtatgg gtcaggaaga agaagctgcc gaacgagaac
    16501 gcgtcttccc gccctaccag gtcaatgacc aactggtgtc tgaagccaat ccggaggtca
    16561 tcgtgatgca ctgcctgccc gcccaccgca accatgaact tacggacagc atcgcggatg
    16621 gaccgcattc ccggattttc cctcaggccc acaaccgcct gcacgcccaa aaagcgattc
    16681 tatatacatt attgagagga gagtaacttg aattcccagg attttatcaa tctcgaagac
    16741 caatacgggg cgcacaacta ccaaccattg gacgtggtgc tgaccaaagg aaaaggcgtt
    16801 tgggtggaag acgtcgaagg caaccgttat ctggactgtc tgagcgcgta ttccgcgttg
    16861 aatcagggtc actgccatcc caaaattgtt caggcaatga cagaccagct ccaacgagtc
    16921 agcctgacct cacgcgcttt tcgtaatgat aaattgggac tcttctataa gaagctctcg
    16981 gacataagcg gttacgaaat gagcctgccc atgaacagcg gcgcggaagc agtcgaaacc
    17041 gccatcaaag tcgcccggaa gtggggctac gctgtcaaag gcatccccaa atatgaggct
    17101 gaaatcatcg tggccgccgg gaacttccac ggccgcaccg taaccatcgt gtccttttca
    17161 accgaacccc tgtataagga cgccttcggt ccgttcacac ccggcttcaa ggtcatccca
    17221 ttcggggacc tggcagccct cgaagccgct atcggcccga ataccgccgc ggtaatgctg
    17281 gaacccatcc agggtgaaaa cggcgttatc atcccgccgg aaggctacct gcgcggcgcg
    17341 gcggatctgt gcaaacaaaa caatgtgctt ttcatcgccg atgaaatcca aaccggcctg
    17401 gggcgcacag gcaaactttt cgcctccgat tacgaaggcg ttcgcccgga catgaccatc
    17461 atcggcaaag cgctttcggg cggcctgtac ccgatttctg ccgtgttggc agataaagca
    17521 atactcgacc tgattcttcc ctccgaacac ggctccacct tcggcggcag cccattagcc
    17581 gccgcggttg gcattgccgc attggaagtc atcgaggaag aaaaacttcc agagcgcgcc
    17641 ttcaccctgg gcaagtactt tatggaccac ctgagtgaaa ttaactcccc gaacatcaag
    17701 gaaatccgcg gaaaaggctt gcttatcggc gtcgaattga aacccgaagc cggcggcgcg
    17761 cgccgtttct gcgagtcgct ccaaagcgaa ggcattctgg cgaaagaaac gcatacgcat
    17821 gtcatccgct tcgcgcctcc gctggtaatc gagcaggaaa cgctcgactg ggcgcttccc
    17881 aggattgcca gggtattgcg acaagcttag aaagcagggt agcatgaaca ggtttgccaa
    17941 gatagttgcc acgctcggtc caagcagttc ggatgaaagc acgctgcgcg agttaattga
    18001 agccgggatg gatgtcgccc gtctgaattt ctctcacggc acgcacgaag agcacgcgga
    18061 gaaagtgaag ctgctgcgca aactctctga agagcttggc aaacctgtct cgattttaat
    18121 ggacttgcaa ggccccaaat tgcgcattgg caaactggag aaggatgtcg tgacgctgca
    18181 gtccaaccag gtcgtcgccc tcagctcctc cgaaaacccg gatcatgtgc gtgaggggac
    18241 aatcttcatc ccgtttgacg tccccaggct gcacgaagcg ctggcccccg ggaaccacat
    18301 cctgatggac gacggtcaat tggaattcga agtcctcgag ctggacggcg agaatatcta
    18361 cgcccgggtc atcctgggcg gaaacctgaa atcgcataag ggcgtaaacc tgcctggctc
    18421 caaactggac attccctgcc tgactcccaa agaccttgag gacctgcagt tcgggctcga
    18481 tatcggggtc gacctggtcg cgatatcctt cgtcaagcag gcttcagata tcctgaccgt
    18541 gcgcgaaact atgcggcgta tgtccagcct gctgcggctg ccgccgattg tcgccaaact
    18601 ggagcgcccg gaagcgattg ccaacctgga atccatcatc gacgcgaccg acggcgtgat
    18661 ggtcgcccgc ggagacctgg gtgtggaaat gtccccggcg gtagtgccct ccgcccaaaa
    18721 ggaaattatc cgggctgcca accacaaggg caaattcgtc atcaccgcca cgcagatgct
    18781 tgaatccatg attaacaacc cgcgcccgac ccgcgcggaa gctgcggatg tagccaacgc
    18841 gatttttgac ggcaccgacg cggtcatgct ctccgcggaa tctgcggccg gcaaatatcc
    18901 ggtgcaaagc gtccggatga tggagtccat catccgcgaa tctgaaaaac acctcaagga
    18961 atggggccac ctggacagcc tggtcagcac aaaccagctg gataacgcga tttccatcgt
    19021 acgcgccgcc aaagacctga ccgttaatgc aaaagtctca gcaattgtgg tgtttaccat
    19081 ctcgggcgcc agcgcgctgt cgatgtccaa gaccaaaccc tcagtgccaa tctacgcttt
    19141 taccccggag atacgcacgt accagtggct gggcatcgcc tggggtgtta ccccgctgct
    19201 tgtcccgcac gccgacacgc tcgaaacaat gatccggcat gtcgaaacag ccatctcctc
    19261 gtccacgccg cttcgcgcgg gcgaccaggt ggtcgtcatt agcggcttcc cggtgggcga
    19321 gtttggcaag cccaacctga ccctgctcta caccctgaga ggatgatcag gcgagcacga
    19381 cttattcgcg acctttgcgc aaatcgagga aaaacacagc aacgcagccg gctgtgtttt
    19441 ttgtttgccg ctgctggccg ccctttcccc atcgaaggag ttccaaaacg agtaccgctt
    19501 gaatattctg gcgggagagt ttaatttcct aaggattttc tattgccagg tttgccctcc
    19561 tgtgatagga ttgctgtgct tggtatataa tagtagataa cttatctata taagcggatg
    19621 aaaataacgc accatggtct taatgagggc ggcaacccct gtcgtcgggg gtccacagca
    19681 gtttgcccac gccagtatac gcggtgtgtt ttgaaagcaa ggaataagag gagaacctgc
    19741 aatgttgaaa tttgatgagg acaagatcat taagagtatc aacggggcgc tggcattgcg
    19801 tccaaaaatt gaaacaatcg ctgatgagtt gtatgaccgc ggttttgatg ctatattcta
    19861 tttgggcatc ggcggtactt atgccagcgg cattcaggtg gtggattccg tggaagggaa
    19921 aagccgcctg cctatttatt tattgcatgc cgcacgttat gccgcgcacg gcaacgcgca
    19981 tgtcacggga caatccgtga ttgtgatcgc atccgacagc ggcacaacct ccgagatgat
    20041 agaagcggtg aagaaagcga agcaagccgg cgcgttgatt gttggattcg tggagtttgg
    20101 cgactccccg cttgccagat tgagcgaccg ccttatctcc tatccgcata acgagcagct
    20161 caagctcttc atgttcgcac atcgcctgat gaaccggaac ggcgaatttg aggactacga
    20221 acgcttctat cgcgaattgg aagcccatct ggccgatgcc tacgtagaca cgtccaaacg
    20281 tgcggatgcc ttcgctctgg cgttcgcgga aatgcataag gacgacccgc tgcattatta
    20341 cgtctcagcc ggcaatcatt acggcgcggc ttactcgtac gccatgtgct acgtggaaga
    20401 acagcattgg attcgctcga aatctatcca cgccgcggaa tttttccacg gcacgctgga
    20461 agtcatagac cgcgacacaa acatcacagt ttttgtgggc gaagatgaag aacggccgct
    20521 ggcggaacgc gtcgcccgct tccttccccg aatctgcgcg cgacatacca ttatcgacac
    20581 caaagattac cccctggaag gcatcagccc tgaattccgc ggcttgattt cgtatcttgt
    20641 gtgtcacgcc gtgaccgagc gcatcgacgc gcacatcgaa aagctaaact gccaccctat
    20701 ggaaatccgc agatactatc ggcggctgga ctactgagtc tccctccagg ccgcagggtt
    20761 aaggtcaggc tggctgatgt gaaataaagc tgtccaaaaa gaacgggttt tcttttagga
    20821 cagctcaaag tgggagaacc tgcctggttc aggcgccgtt ttgctggcgc aaatccgcga
    20881 acatccgtaa aacgggcaag gagtgggaaa accaaaggag ctgtcccaaa agtcggacag
    20941 cccctttagt ttgaatattc agatggaaat cgaacgagcg gccaggggta cctccccccg
    21001 ataaacgcaa tccctgcgcg ctcccctgcc ggctccgctg gcagacttac tggtacaact
    21061 ggttgatgcg ctccagcgca gcaggctccg ggcgcagtct gtccggcgtc agccggttga
    21121 gcggttcttc gacgaaatcg aattcatcgt aaatattggt cggcgcgttt tcgatgtaaa
    21181 tcaggccggt cgcgaaatag tggttcttat aagattcggt cagcaagtcc agcgcggcgt
    21241 tcttatcggt cgggtcgtaa tcccggtcga ttttcttcag gagaatgctt gacccgtcat
    21301 gcagccgcac ttcgcgcgtt tccccttcct gaaagtcttt gatttcaatg gtacgcgcgc
    21361 gcgggacata ggtaatatcc tggatggggg cttcattctc cctgccccag gtataggatt
    21421 gtaatgcttc atcgcggttg ttaaaggtca cgcaggggct gatgacatca atcacagcga
    21481 tgccgttgtg catcactgcc gctttcaata gctctttcat ctgcttaata tcgccggaga
    21541 aggcgcgcgc cacgaacttc gcgccgctga tgagcgcttc catgcacagg tcgagcggcg
    21601 gcaggaagtt cttgccctgg tgcttcagct ccaaacccag gtctgaagta gcggagaact
    21661 gccccttggt cagtccgtac acgccgttgt tctcgatgat gtataccatc gggacgttgc
    21721 ggcggatgat atgcttgaaa tgccccagcc cgatgctggc ggtatccccg tcgccggaag
    21781 tcccgagcgc tttgatgcgc cggtcggcga agcacgcgcc ggttaccaag gtgggcatgc
    21841 gcccatgcag gccgttgaac ccgaaggagc gctgcaggaa gtaattcagg cttttgctgg
    21901 agcagccgat cccgctgaat ttcgccacca tttcgggcat caaacccagc tcgtaaatcg
    21961 cctgttgaat ttgattggag atcgtgttgt gcccacagcc cgggcaaaga gtggagggtt
    22021 tgccgcggta atccaccggg gaaagcccga caaagttgcg ttcggtcctg gcagtttgcg
    22081 tgttatccat tttgctgctc cttgacaata atttgctgct taacccaggc agcggtcata
    22141 ggcaatccgt ccacttcagc caaagaaaag agtttttggg aaaaagcctg caattcctgg
    22201 atgaggatcc cgtacaactg cccgtcgcgg ttcagttcca ggacgtacac gcggtcgtgg
    22261 ttctccagga attcccgcac cgccgcgcaa accggcagcg cgcggatgcg cagataatcc
    22321 gccggaagcc ctgcttgctc gagctggtcc tgaccctcca ccaccgcggg gtccgtcgaa
    22381 cccatcgcga tgatgccaag gcgggcgccg ggtttttccc gcaggattgg ttcgggaagg
    22441 tattcgcagg agttctgcag tttccgtttg attctctgga tcatttccgc ccagttctgg
    22501 gggtcttcgc tgtagtttcc atattcatcg tggccagtac ctcttgtaaa atacggcgcg
    22561 gccgggtttt ggttgcccat aacggtccgc caggggatgc cgtcgccttc gatatccttg
    22621 tagcgccccc agctgccttc aaagcgggcc agatcttctt cccacagaat cctgccgcgg
    22681 tcaatcggtc tgtccgggta ggcgaactta tcagtgatcc attggttcat ccccaggtcg
    22741 aggtcgctca tcacaaacac aggcgtctgg taatgctcgg cgatatcaaa ggctttccag
    22801 ccgaactcaa aacattcgtt cacgctgcct ggaatgagaa ggatgtggtt aatgtcgccg
    22861 tgccccaggc ggtagaccat cgccaggtcg ccttgcgcgg tgcgcgtggg cagcccggtg
    22921 ctgggcccca cccgctggac gtcccagatg acaactggcg tttcggtgaa gtaagccaga
    22981 ccaatattct cagtcatcag gctcaagccc gggccggaag tcgcggtcat cgcgcgcagg
    23041 ccagcccaac ccgccccgat cgctgcgccc gctgcagcca gttcatcttc aacctgcagg
    23101 atgatgcaag tgttttttcc ggtctggggg tcccttcgca gtttcgggtt ttgcgcgatc
    23161 atggattcca ccaggcttga agccggggta atcggatacc aggaacagaa ttgcgcgccg
    23221 ccaaaaagcg cgcccagggc ggcggcgttg ttgccgtcgg tgagtatgca gtccgtcagc
    23281 ggtgccagtt ttcggaccga atagcggtct tgtttggtca aattggcggc tgcccatgca
    23341 aagcccgtct taatcatgtt caggttggat tcacgcgcgt tttgccggct gccaaagtgc
    23401 ttcgtcagcg catattcgat gttctccagc ggtatgccta atacctgccc gacgacgcca
    23461 acatacgcca tgttttccat gtattcgcgc aggtttctgg gaacttccgc ctggcggaca
    23521 aactgccgga ttggcatggg gtaatagacc acgtccgtcc gcgcgtccgc tggcttttgc
    23581 agatggtcag cgtaaaacac aacgccgcct tccggcaggt agctcagatc cttctgaaat
    23641 gtatccgcgt tcatcgctac gattatgtca tcatacggta cccgtccaag atagccgcgc
    23701 tcggagacgc ggattacgaa ccaggtcggc agccccttaa tgttggaagg gaatatgttc
    23761 tttccggatg taggaattcc catattaaag agcgcccgat acaatattgt gtttgccgtg
    23821 gcgctgccgg acccgttcac agtacagagc gtcatgcaaa aatcgtttac ggacgcgctg
    23881 ctctcagcct gtgtgtttgt agtgggatgc tccatctctt tgctgctctc ctggtaatct
    23941 ttattcaaag cgttgggata aatcagcctt tttgcgctgc tttaccagat ccatgtgctc
    24001 aacgagccat tggtaaccgc gctcataatc gcgcgaagcg agcgcatccg caatccggcg
    24061 gtgataattc cacacgtttt ccagataggc cctatccact ggcagattgc gttcgtattc
    24121 cgcgttcagg ttccagaaag tctcgaggat gctgttggcg agcgggtttt ccaggtgctc
    24181 aaatatgagc atgtggaaat cgcggtgttc tggctgcggg gaacgcaccg gttcgcggga
    24241 aatctccgcg aacgcggcat cgacaatccg gtgcagggtc tgaatttgcc ggctggtaag
    24301 ctgcggggca gcctcgtgcc agtagctgat ttcaatatgc ttgcgcaagt cccgcaaagc
    24361 gtcagacacg tccggctgaa cttttgcgcc atagcgcaga caaagttcca gacattgatt
    24421 aatggaaaac tcgcggttct ggatcccgac cctggtcttc acgtccacaa aaccgagcgt
    24481 gcgcgccact tcgagctgct cgcgcacgct ggcaacgcta atccccagca gcgtgctgag
    24541 ttgaggaatg ctggggatag gcgcttgcgc gcgggaagct tcagccagat aatcgagcag
    24601 ttcgttctta cttttcatgc ggggcatata tttaatctga ttaataaaac ctatttatct
    24661 gattatagtc ggaatttctc tttttcgtca aggtgtttag cgctattaat ctgattattt
    24721 tcctgggatt ccaattttcg ggtctacagc gctgaaaaca agaggtctgt tcgttttgaa
    24781 ccgcctaagg ctgagccttt cacgcgcagc ccgagcgccg caagtctgcc gcaatacctc
    24841 aatttctgcc acttttaacc ggacggcaga tttcctgggg tacaatactc aagaattcat
    24901 tcttggttaa tctctcaatg ggtgtcagcg aattctccat tcctttccgt tacaagacga
    24961 acaaggccaa tcctgttgtc tggctgctgt cgcataccct ccaccattgg gctgtgttcc
    25021 tggcagcctt gctgggcgca gtcggcaacg ccgccttggc ttccattccc gctatccagt
    25081 tcggcattgt cttcaccaac ctgaccagcg gcgcgccgga taaaaacgtc ttcatccagg
    25141 cggcgctcgt catcaccttt tcccaggtcc ttcgcggtct gttccagctg atgcgcaact
    25201 tagccttcga ggtcaccgct caaagaatag agcgcgacgt gcgcgatgag ttctacatca
    25261 gtctgcttgg aaaaagcatg accttccact ccctgcagtc ggttggagac ctgatggcgc
    25321 gggcgacgaa tgacattcgc gaggtgaatt ttctctacag tcccgggatt aacatggtct
    25381 tcgggtcgat caactttttg ttcgtccctc caatcatcgc ctggcagatt caccccgcct
    25441 tgctgctgac cccgctcctg ttcattctca cttacttcat tgccgttaca gcttacctga
    25501 agaaactcag acccattacc gacaaggttc gcagcagttt tggacttatg aacagccgcc
    25561 tggcagaagc aattgacggc gtggagacgg tcaaagctgc cgctcaggag gagaacgaaa
    25621 ttgcgctttt ccggcgcaac atcagagcct atcgcgacgc ttccgtggaa cagggcgaca
    25681 tagaagcccg ctttctgccc ctgctgctgc ttgccttggc gatgggcttc ggcctgctgc
    25741 atgcgctggt gctttttcag cagggcacca ttccactggg ggatgtcatt aagtatttta
    25801 gcgcactggc gctgctgaac ttcccgacca acagctccat ccactcctat tcacaaattt
    25861 ctctgggagt tgccagcgcg cggcggctgc tctccatcat gaacaccgag aataatctgg
    25921 ataagaatga cgccggccgc gcagagccgc ttcgcggtga ggtacgtttc acaaacctga
    25981 gcttttccta caatactcac aatacagccc tgcagaatat caacttcgtt gtgaaacccg
    26041 ggcagactgt cgccatcgtc ggtcagactg gctccggaaa aacgacgctg gtacggctga
    26101 tcaaccgcac ttacgaccca agtgaaggtt ccgtgcaaat tgacggctgc gacctaaggg
    26161 actggaggct ggacaccttg cgcagccaga tttctatcat cgaacaggat atctttttgt
    26221 tttccacatc aatagccgat aacattgctt tcagcaaacc cggcgccacc cgcgcggaag
    26281 tggaagctgc cgcccggaaa gcgcaggcgc acgccttcat caccgccctg ccggaagctt
    26341 acgacacgat catcggcgag cgcggcgtaa cgctttcggg cggtcagcgc cagcgcatcg
    26401 ccctggcgcg cgccttcctg acggatccca aaatcctcat tctggacgat tccaccagcg
    26461 caatcgacag ccagaccgag gacgaaattc agcgtgcaat caaggctgcc tccgaggggc
    26521 gcaccacctt catcatcacg catcgattgt cccaaatccg ttgggcggac ctcatcatcg
    26581 tgatgcgccg cgggacaatt tcggatatcg gaacgcatac tgaactgatg cagtcctccg
    26641 cctcttaccg caatatcttc acggagagtt agacagcatg ggattctccg ccggacttgc
    26701 cagcgaatcg tacgaccgcc aatacagcaa taaaacgctg ctcgggcgca tttgggcgta
    26761 cgccaaaccg gaacgcaagc gcattctcgt tatccttttt gcggtgctgc tgcacggcgg
    26821 ggtgggcgcg cttccgccgg tcatcgtcgc caaagtgttg gatgaaggcg tcgccggtat
    26881 tccggatcac caggtgtttt acctgcttgc cgctgttatt atcgtgctgg agctgctcgg
    26941 gtatctgctc tattacatta accaccgcct cctggcgcgc gttatcgcgg atatcaaccg
    27001 cagcctgtcc acagacgctt ttgctgcttc gatgcgccag gatatggctt tccatgatgt
    27061 gttctcctct ggaaaaatcg tctcccgcat cacaaccgat acgcgcgact tctccacgct
    27121 catcacgctc acgatggacg ttgccaacaa cctggtccag tcggtggtaa cctccattat
    27181 cctcctgaca gctgagtggc acctggcgtt attgttcttc ctgaccgtgc cggcatttat
    27241 cctgttcgtc tcgctatacc gcaacctggc gaggcgtgtt tcacgtcagg gcatgcgcgc
    27301 gatggcgaac gtgaacgcta ccatcaaaga aacgatcagc ggaatcgcga ttgccaagaa
    27361 tttccgtcag gaagagcgca tctacgacga attcaagcgc tcgaacattt tgtcctgccg
    27421 gacgaacgtt aagcgcggca tggtcctttc caccgtcttc cccatcactc gcatgcttgg
    27481 cgcgctggcc accgccatgc tggtctattt cggcgcgatt tccgtcacgc aaggggttat
    27541 caccgctggc gcgtggtacc tctttatcct gagcacggac cgtttcctcc aaccaatcct
    27601 cagcatcacc tcctactgga cacaggtcca gaccggtcta tccgccgctg aacggatact
    27661 cgcgttgatt gacgccgaac ataccgtcat ccagaccggc ggatacaatc ctgaaaatta
    27721 ccgcgggaga attgatttca atcacctcac gtttcgttat gccaacggtt ataccgtgct
    27781 gaatgacttc agcctgcaca tccgcgaagg ggaaaacatc gccatcgtcg gacataccgg
    27841 ggccggaaaa acttccatcg cgcgtctgat tgcccgtttt tacgaattcc aatccggcga
    27901 gctgctcatc gacggcaaag acatccgcag ttacgacctg cacgcgctgc gccagcatat
    27961 gggtctggtt acccaggtgc ctttcctctt tgagggcaca gtggaagaaa atatccggtt
    28021 cgcgcggcct gagatcacac gcgaagagat tcttgcgctg gcaagccaga ttggcggcgg
    28081 ggaatggctg gacactttcg cgaacggcct ggacacccgg gtcggggagc gcggggcgca
    28141 gatttccatg gggcagcgtc agctcgtcgc gctgatgcgc gtactggcgc aaaatccctc
    28201 ggttttcatc ctggacgaag ccaccgcgag catcgacccc ttcaccgaga agcaaatcca
    28261 gaccgcgctg aatctgattc tcaatcaatc tacaagcgtc cttatcgcgc accggctctc
    28321 aaccgtccaa agcgcggacc gcatcatcgt cctgagggat ggcgacatca tcgaagaagg
    28381 cacccactcc cagctgctca agcaaggcgg gcattactcg gagctgtaca acacctactt
    28441 ccggcatcag agcctggact acgcagaaca tgcgggcgga tgagctgcct gcgtaccgct
    28501 gctcgtccgc acagcgctct cgcttgactt taggctttat ccggctaaaa ttaaatcaga
    28561 acattggcag ggaagagccc tgcttcaggc accgaagggg caaacccgca aaaacgggcg
    28621 aaactctcag gtaaacagac cctgccaatt aatcctctga aggtaacagt caacagttaa
    28681 cgacagaggc acaagaaacc tgtgcctctg caactatttg ataaggagaa acgatgaact
    28741 tcccacaaga actaaaatat gctcagacag atgaatgggt gaagcttgaa aacggcattg
    28801 ctaccgtcgg catttcggat tacgcccagg atgcgctcgg cgatatcgtc tatttcgagt
    28861 tctcggttga ggttggagat catgtgcagg ccggcgggca gctcggtcag gtggattccg
    28921 ccaaagccag ctcggaggtc atgctgccag tgagcggcgt ggtgaccgag atcaatgaag
    28981 ccatcctcga tgaggtggaa atcctcaacg acgacccata cgggaaaggc tggctgctcc
    29041 ggattaaggt cgacgatcct gctgagctgg aaaagctgct cagcgccgaa gcctatcggg
    29101 cctaccgggt agggtaaagc ctatgttcac gccccacacc caaaaagatg tggaggtgat
    29161 gcttaagact atcggaatcg gaaaagttga agacctcttt aatgagatcc cggaaaagtt
    29221 ccgtttccca gagctcaagc ttcccgcctc cctgaccgaa atggaagctg ccgacgagct
    29281 ggaaggcatc gccgacgcga acggcagcac caaaaccttc ctgaacttca tcggcgcggg
    29341 cgcctacgac cattatgtgc ctgcggcggt ggatatgctc ctgcggcgcg gggagtttta
    29401 caccgcctac acgccgtacc aacccgaaat ctcccagggc acactgcagg caatttttga
    29461 gtaccaaagt ctgatttgcg agctgaccgg catggacgtc gccaacgctt cgcattacga
    29521 cggcgctact gccgcggcgg aagcagtgat cctggcttac catcacttca aaggcaagcg
    29581 caccaaattc ctgatatcgc gcgcggttaa cccgcagtac cgcgaagttg tgcgcaccta
    29641 tatgcgcggc agtcaggaga tcagcatcca gggggacgat cctgaaaccg gcatcgacgc
    29701 ctcgatggag aaactcatct cccggattga ccccaacacc gccctggtga tggtgcagta
    29761 ccccgatttc ttcgggcgcg tgatagatta caagccgctc atcgcagccg cgcacagtgc
    29821 cggcgcgctg gtcgcggtcg ccgtcaaccc caccgcgttg ggtgtgctca cccctccagg
    29881 tgaaatcggc gcggatattg tctttggcga aggtcagccg ctcggtattc cgctctccta
    29941 cggcgggccg tatctgggta tcttcgcggt tacgaacgac ctgcttcgca agatttccgg
    30001 ccggcttgtc ggtgaaactg ttgacagcgc cggcaggctg gcgtacgtgc tcacgctcac
    30061 agcccgcgag cagcacattc gccgcgagaa agcctcttcg aatatctgca caaaccaggg
    30121 attaatggcg ctggcttcca ccatttacct gagcctgctc gggaagcagg gctttgaagc
    30181 cgtgagcaac ctgtgcttcc aaaaagcgca ttacgccgca aaacaaatca gttccatcca
    30241 gggctatacg caggagtttt ccagccaacc cttcttccat gaatttgtca tccgcagccc
    30301 gcagccggtg aagcacctgc tcgaagactt gcgcgcctat gacatcctcg gcggttacga
    30361 cctcggcagc gattatcccg agctggaagg ctgcctcctg atagccgtca cggaaaaaat
    30421 aggcaaggaa gatattgacc tgctgcacga cgcgctgatc gaggtgaccc atgactgagc
    30481 ctaccatctt tgaattgtcc tctgcaggcc gccagggcgt gcgctttccg gcttcggacg
    30541 tgccggaggt ggaactgccg gctgccttcc gccgcaaaga actccctctt ccggagctgg
    30601 cggaaatcga cactgtacgc cactttacgc gcatctccca gaagaattac gcgattgacc
    30661 tggggtttta cccgctgggt tcctgcacga tgaagtacaa ccccaagatt aacgaagtta
    30721 cctcacgctt gcccggcttc gcgctcactc acccgctgca gccggaagac acggtgcagg
    30781 gcaacctcta cctgatgtac accttgcagg aatggctgaa ggaaatcgtc ggttttgccg
    30841 gcgtctccct gcagcccgcc gccggcgcgc acggcgaact gaccggagcg ctgatcatcc
    30901 aaaagtacca cgccgaccgc ggtgacagtc agcgcacgcg catcctcatt ccggattcag
    30961 cccacggcac caaccctgcc acctccgcga tgagcggttt tgaggtggtg aacctgcctt
    31021 cagacgcgcg cggaaatgtg gatctggaag ccctcaaagg cttatgcgat gaacgcctgg
    31081 ccggcttgat gctcacgaac ccgaataccc tcggactttt tgatgagaat gtgaccgagg
    31141 tcatcgagat ggtgcacgct gccggcgggc tggtctacgg tgacggcgca aacctgaacg
    31201 ccctgctcgg cattgtccgc ccgggggatt tgggattcga catcatgcat ctgaacctgc
    31261 acaaaacctt ctccaccccg cacggcggcg gcggcccggg ctcgggtccg gtcgcggtct
    31321 gccagcggtt ggtagaatac ctgcccgacc cggtagttgc gatagtggaa cccgggagcg
    31381 aggaactccc gcctttatat ggctacgcgc accccgcgaa atccatcggg cgcgttaagt
    31441 ccttcaacgg gcatttcggc atgttggtcc gcgcttacac ctacattgct atgcacggcg
    31501 ggaacggcct gcgaaacatc gccgaaaaat cggtgctgaa cgccaattat ttgcttgcga
    31561 aggtgaaaga cgcctaccat ctggcatacg accgccgctg catgcacgaa tttgtggtgg
    31621 aaggccactg ggcggacgcc cccggcatcc gcgcgataga tattgccaag cgccttatcg
    31681 actacaactt ccatccgccc acaaactact tcccgctgat tgtgcacgaa gcgctcatga
    31741 ttgagcccac tgagaccgag tcgctcgaaa cgctggacgc cttcgcggac gccatgctga
    31801 agattgccgc ggaagcgcgc gaaaacccgg aattgctgca cgaagccccg cacaattccc
    31861 ccgtcgggcg ctgtgacgaa gtccgcgctg ccaagcaatt aatcttgcga gcgccgaaag
    31921 tttaaatcca ccaggaatca ggggctgtcc aaaaaatcgg gcagcccctt tggttttccc
    31981 actccgtgct tgtttttccg gggttttgcg gctttgcgcg gataatacga cgcctaaacc
    32041 aggcactttc tcccgccttg ggctatccaa aaagaatcac ccgtactttt tggccagcct
    32101 cttgggttta acacagctcc agcgttaccg ggcagtgatc ggaacccata atctcatcca
    32161 ggataataac atcccgcacc cgggtcatca gaggcgcgct gaccaggaaa taatccaggc
    32221 gccaaccgat attcttgacg cgcgcgttca tgcggtaaga ccaccatgta taagccgctc
    32281 tgagcggata aagctcccgg aaaacgtcga taaagccgtg ttccaggaac ttatcaatcc
    32341 acacgcgctc ttcaggcaaa aaaccggtgt ttttttcgtt ttcctttggt cgggcaaggt
    32401 caatttcatt atgcgcggta ttgaaatcac cggtgatgat gacattttcg ccatgctcat
    32461 ggtgttcctc gcagacctcc agcagccggg cgtaaaagtc cagcttgaaa gggacgcgct
    32521 ggttttcttc cccgccgttg gggaagtaaa tattgtacag aaaaaaatcc ggatatttca
    32581 gccgggtcac gcgtccctca gtatcaaagg cttctatgcc cagtccagtg ccaatctcca
    32641 ggggcggctc tttgtaaaac accgccgtgc cgctgtagcc cggtctatcc gcagggttcc
    32701 agaagctttt atagccctgt aattcgcgct gttctccact caactgctcc ggcttgacct
    32761 tgatttcctg caggcacaag acgtccggtt gatgctccat gacccaatcg aagccctgct
    32821 tgcttagggc agcacggtat ccgttcacat tccaggtggt aatcttcata ccttttctcc
    32881 tttttcccgg cgcgcaaggc tatggtaaac ttagggtttg ataaatatga cccgacgaaa
    32941 attacatttc cctttacaac gcattcggtt tctgttcctg ctgattttac caatgatttg
    33001 cgcggctttg gccccggcca gcgtaaaagc gcagtccgcg cagctgccgg tctacatcgt
    33061 ggagtccgga gacacgctct acggcatcgc cgaccagttt tacacgacca ttgatgaact
    33121 cctggccgtg aataaccgca gtatcgggga tacactgcgc ccgggcgacc ggttgtatat
    33181 ccccgggctt gaagggctgc aaggcgtgct cagcacggat tacgttccgc ttggcgccag
    33241 cctgcgcagc ctgagccgcc gcacgcagtc cgaccccgcc agcctggtgc gtctgaacaa
    33301 gttcacaagc caatcggagc tctttgttgg tcggaaaatt gcgctgacta cctcggaaac
    33361 cacgcaggac ctgcaaacaa tgccctccct cctgccagga caatcctgga tggaacgctc
    33421 cattctcgcc ggtcagaatc catgggcgct tgcccgcttg aaccggctgg gctcgccaaa
    33481 cgccgcgctc ccacaggatg cttactacgc gcactccgcg caaagcaacc caaacagcct
    33541 ggctatcccg ggcattacat ccctggtaat tggcaatctc cccttggttc agggcggcac
    33601 cttcgtcctc gagcttacaa gcgagcagcc agtctcactt tcggctgatt tggcgggcgt
    33661 tcaaccggtt ttctttaccc aggataacgg cagccagata gcctttggcg gcatcaacgc
    33721 tttacaagag cccggtgcct accccctcac catcgaagtg acgaatgccg atggcgcgac
    33781 ttaccgcttt gaccagtgga cgattattgg ttcgggcaat ttcgagaccg atgagacgct
    33841 taaggtggac cctgaaaccg tcaacgggga caacattacc gcagaggatg ccctcttcaa
    33901 gcagattgtc acagtgctca cgcccatccg gcagtggagc ggagtcttcc agtaccccac
    33961 caagggcggc gactgcgtca attcgcgctt tggcagccgg cgctcttaca ccggaaccga
    34021 caagatttac taccacaccg gtctggatat cggttggtgt tacggtattg acgtgttcgc
    34081 cccagcggcc ggcaccgtgg tggctgttct cccagagcaa attgtgcgcg gtaacactct
    34141 cgtgattgac catggattgg gcgttttttc gatctatatg cacctgcagg attttctggt
    34201 agcggaaggc gaccaggtac agcccgggca gcttattgcg catatcggca acaccggacg
    34261 ctcgagcggt ccgcacctgc attttgaaat tgacatcaat gggacgccgg tcaacccggt
    34321 gatttggttg aaccgcgaat ttccataaaa aaagcaggca ttcctgcctg caaggtgcgc
    34381 tgggggggac ttgaaccccc acggcttgcg ccacatggcc ctcaaccatg cctgtctgcc
    34441 aattccagca ccagcgcgtg gatagaaatt atatctgacc tgaacttatt gtcaaggaat
    34501 ttagttagag aggaaagcta tgaaaacaac aatcgtgctg gacgcgatgg gcagcgacaa
    34561 acgtccgcag ccagaagtgg aagcctccgt gcagtgcgcc gaaactctcg gcgtagacct
    34621 cattctggtt ggcgacgaag ctgtcctgaa acccttactt gccgcccggc ccggggataa
    34681 aacgcacgtg cgcatcgcgc acgctcctga agccttggag atgagcgacc acatcgccga
    34741 agcccgcgcc aaacgcaata acaccatgca agtcgggatg gatctggtgc gcagcggtgc
    34801 ggcacaggcg tttgtcaccg ccggtaacac cggtatggct atgtattttg ccacaaagac
    34861 cttcggtctt attccgggtg tcatccgccc cagcctgtgc accacattcc ccgtaaaaaa
    34921 cggaaaatgt gtggttttgg atattggtgc caacgcggaa tgccggcctg aatttttagt
    34981 acaatttggc cggatgggca gcgtttacgc ccgtctgctg ctcgggcgcg accaaatccg
    35041 ggtgggctta ctggcaaacg gcgaggagga aggcaaaggg aacgagctgg tcaaagcagc
    35101 cggaccgctg atgcgcgctg aagtccccgg atttgtcggg aacatcgaag ggaaagaatt
    35161 atttgccggc gaagtagacg tggctgtgac ggacggcttt accggcaaca tcctgctcaa
    35221 gtccaccgag gcggtctccc gtctgattct tgaaacgctc aaggaagagc tgctgcgcag
    35281 cacccgcacc aagctcggcg cggcgctggc caaacccgct ttcaattccg tcaaacaaat
    35341 gatagaccct tctgaaatcg gcgccgcgcc cttgctgggc atcgatgcgc tggtattcgt
    35401 cgggcacggc cgctcggacg ccaaagccgt gctgagcgcg gtaaatcagg caaaaatcgc
    35461 tgtggaatcc ggcttgctgg actccctgcg tcaatccatc ccatcagata acagtggaga
    35521 atcacactca tgaaactcat aatcaacgaa aaactcatta agaaaaacaa aaccatcggc
    35581 caaatcacca cgtttgccag cctggccgta ctggccatcg ggctcgtgtt tgctttcggc
    35641 aaggacatga ccaaaatcct ttattcctac attgccctga ttgtcggttt cgtcctttcc
    35701 caggtcggca tgtactactc tacccgcttc gggcgcagtc cgcggtttga tgagttattc
    35761 aatgacgctt tcgataaact tcgccatgag tacagcttct atgtctactc cagcccggtg
    35821 ccgtatcttt tgcttggacc ttgcgtcatc tgggtgccga ttcccttaaa cgccagcggg
    35881 aagatttcct acgaaaatgg caaatggaaa cagcagggcg gtaactttat gatgaaaatc
    35941 ttcggtcagg aaggaatcgg ccgacctgtg caggaagtaa gcgccaacac tgccgctctt
    36001 cgtaattacc tgactggcaa aggcattcct gaagaagagc agccggagat taggcacatc
    36061 atggtggtca tgatgaaaaa cacaatcatc ggcgaagtca gccaagcccc cattccggtc
    36121 gtcaacctgg aagatctcaa gcgctccatc cgccgcgtgg accgggaaga atgcgagaaa
    36181 ccgctgacgg aagaacagct cgcccgatta accagcgcgc tggacgagta ttccaagaaa
    36241 aactcctgag aaatcaggag tttttttcgt ctttatccaa acctgcgtat tgacgcaggc
    36301 gtttcacttc cgctgggctt aggtagcgcc actcacccga acgcagggag cccagcttta
    36361 gcgtgccaat ccgtacgcgt ttaatttggg ccaccggcag cccaatgcga gcgccggtct
    36421 tccggatttg gcgttttttg ccttcccgca tggtaatccg cagccaggta ccacctccgg
    36481 cgttattcat cacttccacc ttggcgggca aggtccggta gccgtcctcc aggacgacgc
    36541 cgcgctgcca gatggccagc tgctcctcgt ccggctgctt gatgacgaat acctcgtatt
    36601 ccttttcgtg cttgtagcgc ggatgcgtga gccggttggc cagatccccg tcgttggtca
    36661 tcaaaaccaa cccctcgctg tccaggtcca gcctgccaac cgcgaataag ggctccttca
    36721 gcccgaccag gtcgttcacg gtcggcttgg ggtggtcttc gtcgatgtcc gagagataac
    36781 ccttgggctt attcagggcg atatatatct tgtcttgctt caggcgcagc agtttgccat
    36841 ccaggctgat gctgtcccgc gcggggtcag ctttatcgcc caaatggatg accttgccgt
    36901 tgaccatcac acgcccggct tcgataaagg tctcgcaagc tctgcgggaa cccaaaccag
    36961 ccgccgccaa aattttctga acgcgttctt ctgccattag tcttttaata tcctttgttc
    37021 ctgcggttga gccgctttca aggcgtcaat atccatttca ggcagctgtt ccaacgattc
    37081 cagaccaaaa tgcagcagga agtccggcgc taccgcgtac aaaatcggcc gcccgggagc
    37141 ttcagacctt cccttttcct caatcagccc cttcgcgagc aggctcttca ccaccgtatc
    37201 gctgtttacg ccgcggatgg cttcgatttc aggccgcgtg gaagccagtt ggtaagcaat
    37261 aatcgccagc acttccagcg ccggctggga aaggcgtgag ctgacctcca agcccaggaa
    37321 ttgctcgatg agctgcgcgt gttccgccgc ggtgaccaac tggtactggt tccgaatctt
    37381 ctgcaggcgg attccgtggc tttcctcata atgctgttcc agggtcgctg ccagctgctc
    37441 aagctccgcc gggctcatct ccaaagcctt tgccaggtct tccaccgggc ttagtcctgg
    37501 cgcggcaaac agcagcgctt ccaggcggct gagattggtc ttggtttcgg ggctgatttc
    37561 cacttctgtg ctcatgctat aatgttctca cgaaagttac gtgattatac tctacttatc
    37621 tcggggaaga agccccgaag ggatgcactc atgcgcgtgg cactcaccgg cgctgccggt
    37681 tttttaggct ctcatttggc ggacagactt atcggcgaag ggcacgaagt catcggcctg
    37741 gataacttca tcaccggcga cgaagccaat atcgcgcacc tttcaaaatc cggcagattc
    37801 cgattaatcc gtcaggacgt ctccaccttc atcgatattc ccgaaagcct ggattttgtc
    37861 ctgcactttg cctcccccgc cagtccgaac ccagcctcac cgttcggcta tcctaacctg
    37921 cccatccaga cgctgaaagc cggcgcgctg ggcacgctga acacgctcgg cctcgccaaa
    37981 gcccacaaag cccgttacct gttggcctcc accagcgagg tgtatggcaa cccgcaagtg
    38041 cacccccaaa cagaaagcta ctggggcaac tgtgatccga ttggcccgcg ctcggtgtac
    38101 gacgaagcca aacgcttcgc ggaagccctg accatggcat acatgcgctt tcataatctg
    38161 aatacccgca tcgtccggat tttcaatacc ttcggaccgc gcatgcgcct ggatgacggg
    38221 cgcgtggttc cgaatttgat ccagcaggca ctgcgcggag aaccgctcac aatctacggc
    38281 agtggctccc aaacccgcag tttctgttat gtcagcgacc tggtggaagg catctaccgc
    38341 ttgatgctca cggaagagca tgaaccggtg aacctgggca acccgaacga aatcagcatc
    38401 cgcgaattcg cactgctgat taacgaactg accggcaacc cggccgggct gataacccgc
    38461 ttcgcggaag gtctgggtga tgacccccag cgccgccggc cggatattac ccgcgcaaaa
    38521 acccttctgg gatgggaacc ccaggtggat ttaaaaaccg gtctgcatga aaccatccgt
    38581 tacttccaga agaaagaccg ggcatgacca aaaaatccac tccggaggct ttgcgcttcg
    38641 gaaagtttct gatggtcggc gcgctgaaca cgctgattga ctttgggctg atgaacctgt
    38701 tctccggctg gctgcgcatg ccgctgatgc ttgcccaggc gctctcattt ctgctggcag
    38761 ttatcaacag ctacgtgttc aaccgcctgt gggtctaccc ggaaacacaa gagaagtctc
    38821 tcgctggtca gttttcaaag ttcgcgctga taaacgcggc cgggttggcg ctgcgcacgc
    38881 ttaccattcc gcctgtggac agctggttca ggcaattgat cagtaacaac caaatccaac
    38941 tattcggtct ctcacctcag gcgctgagca ggaacttcgc cctggcggtt atcctgcccc
    39001 ttaccttgat cctgaactac ctcgccaacc gctactggac tttcggagac attgtcatcg
    39061 cccagtccgg gaacaagaaa gcgcaggtct gatgggaact ctgcgcgtta tcggcgggag
    39121 cgcaaaagga ctgcgcctga agactgtacc gggggatacc acccgcccca tcaccgactt
    39181 agtgaaggaa gcgcttttca acattcttgg cgatgaaatt tcaggcagca aggtgctcga
    39241 tttgttcggc ggcaccggtg cggtcgggat tgaagcgctc agccgcggcg cagagacagc
    39301 cctctttctg gaaaaaagcc gggaagccgt acgggtaatc caggaaaacc tgcgcactac
    39361 gcgcttgggt gagaaagcgc aggtgctgca tcaggacgca ttcactttcc tcgcgcaggc
    39421 gccagcaagc gcttttgacc tggtttacat cgcgcccccg cagtacaagg gtatgtggca
    39481 gaaagccatg gatttattgg accagcagcc gggattcctg aaccggggcg ggcaaatcat
    39541 cgtccagatt aacccggtgg aatgggaaga gcgcgcttac ctgcatttta cggagtttga
    39601 ttcgcgcaag tacggggata cacttctggt ctttttcgaa cccaatcagg ctgaaaacga
    39661 cattaccaac tcttcgcgcg cgtcgtagcg gtacacgccc caaaatggct gcccgcccgc
    39721 cgaagtccgc gccgctttgc ccaccaaaac cggcacatct tccaccagcg ggaactggct
    39781 gagctcctcc aggctgaacc agcgcaagct gccttccgcg ctctcgagca gttcacccgc
    39841 cagcatttcc gcccggaaca ctgagaaagc aattccggtc ttttcccggg tgtccaccaa
    39901 aacctggccg caaaaagtcc agctttgtgc tgtcaatccg ctttcttcca gcagttcgcg
    39961 ccgggcagcg tccagcacgc tttcgccggc ttccacatgc ccgccaatgc cgttccacaa
    40021 acccgcccag atgcgtttat gcgccgcgcc gtgcaaaagc agaaccagcc cgtcaacgtt
    40081 gaaggggaaa acaagcaccc tcgggataac cttgtagcga tccgcgctta ggccttgtgc
    40141 tgctattccc atgatcctcc tgaaaaaaac agctcataaa acttttcagt tttatgagct
    40201 gtcagaacgc cgtttcctca gacaaggaac attggcaggt tatgaatatc ctggatatgc
    40261 gggcggtaat tctctttgtc gtaatacata tccacatcca catggcgttt gccctggatg
    40321 gaagaatcta tcggaacggt ggtgtagacg ccattattaa tcgcgaccat ctttccaaat
    40381 tctttcctgg cggtcaactg cattgccagg gttccgtaat tcaacgccac catgcggtcc
    40441 agcatgtccg ccgggccgct gcgcaccaga taggccagct tctggaacat gatatgctga
    40501 cctgtgagcg cattgatctg atctccgacc aattcgccga tgccgcccag tttcttgtga
    40561 ccataggcat cttcttcgcc gctttccacg atcacgccgc cgtccgaaac cgctccttct
    40621 gagatcacgc acattgagta gttcgatggg tttttcttct tgtccgcaac caaaaagtct
    40681 gccagcttgt ggaagttgaa cggcacttca caaataatcg ttcgatccgc aaaactcagg
    40741 taaccggtca ccaggctggt ctctccggaa tttcgtccga agagttccac aatcccgatg
    40801 cgttcgtgcg agccgaccga tgagcgaaag ttggtaatca gcgaaaccga ttgagtgatt
    40861 gaggtggaaa acccgatgca gtattccgtg ccgtagacgt cattatccat cgtcttaggg
    40921 atggcgacta ccgggaaacc ttcctggtac aggggggcgg agtagctgag ggtatcatcg
    40981 ccgccgatgg taatgaggga gtcgagaccg tgataatcca gcaccttcag aatgtaatcc
    41041 gtgaaatctt ttgtcccgtc aggtttcaca accttgccgt aagcgctgtt cagcaggaac
    41101 tccggggtgt ccttcggccg cacgttggaa gggttggtgc gggaggtatg caggaaggtg
    41161 ccgccggtgc ggtccacttt gcggactgaa tttacatcca atggtctgat gaggtctgcc
    41221 gtactttccg ggttgtccag gttatagcgc agcaagccgc cccatcccag gcgaatcccg
    41281 atgacctcgt aatctttttc gaccgcgttc agaactactg attttattgc caggtttaaa
    41341 ccagggacgt caccgccccc ggtcatcacc ccaatgcgtt tttttgccat aatttacctc
    41401 caaaataaag aatatccaag cgaaagaacc tgctaggcgt cgcgcagagg tttcgggctg
    41461 ctcatcgccc accaacgggt ccatcccaga caaaccaatg ccgcaatggg cagggctgtg
    41521 atgacccagg ttgcctcttt ggaggaccaa ttttgagtgc gcaggaatac ttgccagttg
    41581 ctgtagagca aaagcaaaag gattgtccaa aaaataattt taccagaaat cttccatttc
    41641 tcttgaagaa aatcgtaaaa aatcagaaaa ccaggccagg ccaggattaa aaacgcgggg
    41701 gaaccggccg ggttgaacaa actgagcagc gtcagcgtga gcatcagctt ccaacgaaag
    41761 cgcctttcat cgttttccgc cagcccgtac cattcaacca gcatcgcgaa cagcagcagt
    41821 acgtgcagcg ccgccgccaa gggcactacc gcgccgggga agaaggctgc cagacgcatc
    41881 agtggcgtat ccagccagtc aaagcgcggc tgcgccgcaa tcaggctggt gaaccactgc
    41941 gggatccacc cgggaaacag aatcaacgat gtgagcgcca aaaataccaa tccgaccagg
    42001 aacacaggca gcgcgtctgt ctgtttgcgc gcattcagcc agatgatcag gaaaaccgcc
    42061 ggaagcaagc tgacaggcag catgccgaca cacagaaaca aaagcacgcc ggcagatgta
    42121 cctttgccag ccagcgcgct gcggcaggct gccagcagaa caaaaataaa cagcggaagg
    42181 atgcttgcgg ataggatcag cttgaaaaac gggtaagaac aggtgattaa cagtgcaagc
    42241 agcacacgtt ccaggctgcg catgcgcgcg ccagccaggt caatcccgaa ccaggcgctg
    42301 aaaaagatgg cggctgtggt cagagtcatc cagattgccc gcgcgatggg ataaggtaaa
    42361 aagctgaagg ggatgtagag gaatacatac cagaccaggt cgataaacct gccctggctg
    42421 aaagccggcg ctgtgtagcc cagttgatcg taaagcgcct gggcttcctg gtaggcctgt
    42481 tcagaatatg gggaggtccc gtcgcgcatc caaccccgcg ccgcggacca acgcggcagg
    42541 agctcgtcct ggtacaaaaa ttgctctgat aactgcaaat taagcagaat cagaccggcg
    42601 gcgagcgcca caaccaggag aggaacccaa tattgagcgg tctttttcag aagcaacatt
    42661 acctcgcgac taataccgga ttttaatcgc tgccagcgct gcggtcaaat atccgggatt
    42721 gtataagaaa agaccttctt aaatatggct taaaaggata taataagcaa cttgctggca
    42781 aaaaacaaag gtttgaaaaa cttgtgaaaa cacttgaatt tgtctccgaa atattgtact
    42841 ctatgggtta agtgcgataa tttgtataga aaaggtacca ataactgaat ggcaagaatc
    42901 aataatgata atcaagttct ggacgacgat cgtgaatcct cagcgcttga acgcctcctg
    42961 gatttcggaa gaaaaaagac ctatgtaacc tacgatgaca tccttcagtt ttttccggat
    43021 gccgagcagg acctggatca acttgaagaa gcttataccg ccttgcttgg cgcgggcatc
    43081 aactacacgg aagaaacctc agcgcaggat gaacccagcg agacggaact ttcggacgtg
    43141 gccagcgagg aaagcgcgct ggatgacctc gccaatattg acacagacga tactatcggg
    43201 ctgtacctga aggaagtcag ccgtgttcct ctcctgcatg atatcgaaga agaacagcag
    43261 ttagcccaac gcatcgagcg cggacgcgca gcacgcgaag acctggccaa gggcaatatc
    43321 ggtcccaaaa agcgtctgga actgcgcgcg ctgattgagg acggttgggc cgcccgcgag
    43381 catctcatca ctgccaactc ccggctggtc atcagcgtcg cgaagaaata tatgggacgc
    43441 ggtgtgccat tcctggacct gattcaggaa ggcaacatcg gtctaatccg cgcgacaaag
    43501 aaattcgaat accagcgcgg tcacaaattc agcacgtacg ccacctggtg gatccgccag
    43561 gcggtcaccc gcgcgattgc cgatcaggga cgcaccatcc gcgtgccggt ccacatgggc
    43621 gatcaaatca ataagctcct gcgcgtccag caccagttaa cccaaaaatt gggcagggac
    43681 cccagcgtgg aagaactggc ggaagccctg gaagtgccgc cgaaaaaggt tgaaaacatg
    43741 atccaggtcg cccgccggcc gctttccctg gaaaccccga cggatgacga agaggattcg
    43801 gtgctgggtg atttcatcga ggatgacgaa gctcctccac cggatgacac tgccacctat
    43861 aacctgctgc gcgcgcatct ggacgaagtg cttgacaccc ttcctccccg cgaggtccgc
    43921 atcctgcagc tgcgttacgg tttgctggac gggcaagctt acacactgga agaagtcggc
    43981 cgcaagatgg gcgtcacccg cgagcgcgtc cgtcaaatcg aagcccaggc gctgacccgt
    44041 ttgcgccacc ccagcgtgcg ccgtaagctg cgcgattacc tcggagagta aacacacctg
    44101 cagccggctt gctctggctg cagtttatta aacaggcggc tgtgatggaa agcgtaaacc
    44161 ctgccaacaa acacaccgaa ccgaaagcga ccggctcccc tcaaccgctg cagatcagca
    44221 cccccgaccc gcgcgaagcc accaggccgg tacgaattgc gcaaagcaag ccagccaacc
    44281 tgggcgtcag cgcgcagcca atggcagcga ccgcgccaga gttggaaaac accaggcctg
    44341 tccaaatccg cattgtacca gcgcaaacac cccctacagc aaccccgggc gatgaattgc
    44401 ctgcctggtt gataagcttc gccgcgcagg aaaccaaacc cgctgcgccg gccaatccgg
    44461 aagaagatac gcggcccata cctctgcagc ttccggacga agctcccgaa ccagaagcag
    44521 ccgtgatacc ctcacatgct tcaggattgc aagcgccgga agccgataat ggctggaccg
    44581 tggaaatcat tccggaaccc ggcgcgcagg aaatcaatct tgataccgcc gcgcaaccag
    44641 acgcgggaga aatagggatc gaaagccgtc cggaaactgc ggctgcagag caggaagacc
    44701 ttcccggaga ggttggggca gcgatggcag aagacattat tccacaacct tctggcagcg
    44761 gcggacttgc agcagagaca tctgcgccgc caggcaatcc tgctcttgaa aattctcagg
    44821 cgttctcgct ggaaaaagcg ctggatgaaa gcaggattga ggaagccggg gccatgctca
    44881 tgcatatgaa atcagacccc gccgcgcgcg catgcgccct ccaggtgctg cgctcacgcc
    44941 tggacttgcg cgcggagagt ctgccgttgt ggcagtatta cgcggaactg tgcagcgcgg
    45001 ataatcaacc cgggctggcc aggcaggcgc tggaaactgc tgaaaaacta aaaatcgcat
    45061 ctggagaaga acatggaacg atcgctggta ttggttaaac cggacggcgt gcagcgcggt
    45121 ctgataggca aagtcatcac cgcgctggaa gaccgcggtt tgcgtctgac ggccgcccgc
    45181 tttttacagg tgaaccggga attggccgaa gcgcattacg ccgttcacgc gggcaagcct
    45241 ttttacgacg cgttgattac cttcatcacc tcttcgccgg tcatggcgat ggtctgggaa
    45301 ggcccggacg cgattgcggc cattcgccag accatgggca agacgcgcgg aacggaagcc
    45361 gcgcccggtt ccatccgcca cgactacgcg ctccaggccc gttataatat tacccacgcc
    45421 tcagattccc ccgaaaacgc ggaggcggag attgccctgt ggttccgacc ggaagagctc
    45481 gtccgctggt cgcgcgaact tgagaaatgg atttacgaat gacagaacat ggtttatcag
    45541 atttggtcgt agtgtaccgc gccaagggct tggttgacgg tgaaattatc aggggcttcc
    45601 tggcagccca tgacatcccc gccagcgtga gccaggaagc cgccgggaaa atttacggac
    45661 tcaccatcgg ggatctcggt gtagccgagg tgttggtccg cgcgcaggat gaacagaacg
    45721 cgctcgagct cctgcgggag atggaa
//
LOCUS       DAOQAD010000005        44849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_690_length_44849_cov_6.541590, whole genome shotgun sequence.
ACCESSION   DAOQAD010000005 DAOQAD010000000
VERSION     DAOQAD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 44849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44849
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_690_length_44849_cov_6.541590"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1053)
                     /locus_tag="PKL60_00910"
     CDS             complement(19..1053)
                     /locus_tag="PKL60_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNW94724.1"
                     /translation="MNTPENRYFAITKSGTLHRASGLDDALLFAGREGYVWCDYIDPN
                     AESLSELETKLGIHPLSIEDCLNGDQLPKLDLFPTYSFMTFSIFERDTDGLHAHEMDL
                     FISKQYFITVSSRDLNGNSVLAGMEASVTREMDAVRLGPSYLLHQVVDMIVDHKLLAV
                     ESIEEKLDSDEDAILRDAASYNLGALMDSRRDLQVIRKSLFHERELVGKLVRKDSPFL
                     AEKSLIYFRDVYDHLSKYYEIAETAREQVTNLMEIHLSLISNAMAESSNRTNAIMRRL
                     TLITTIFMPLTLISGIGGMSEFTMMVGPTNWQTAYLVLLIVMVIVGAINYALLLKMER
                     SAEQQYRNKP"
     gene            complement(1129..2151)
                     /locus_tag="PKL60_00915"
     CDS             complement(1129..2151)
                     /locus_tag="PKL60_00915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW94725.1"
                     /translation="MEKPLTSPAALAAEPVPFFSVVMLHWNSGSFLAAALDALEEQTY
                     RDFELILLDNGSPDALDANMLAQHARLPLTVLRSERNLGFAGGNNLAAKYARGEYLVL
                     LNADAFPRPEWLAEIHAAAQKRPGACFASRQLTAGDPDRLDGEWNVYSAAGLAWRKSH
                     GQPLARACPSEREVISACAAASAYPLAAFREAGGFDEDFFAYLEDVDLDLRLRLLGYP
                     CYYLPRAVVRHIGAGSTAVRSDFSLQYSHRNLIWTYVKNMPGAFFWLLLPAHLLVNLA
                     YLLAAALTGRGRLIARAKWESLKTLPAVWQKRRKVQGSRRVSAARFARLLNWNPFAPL
                     VKLLCR"
     gene            2206..3030
                     /locus_tag="PKL60_00920"
     CDS             2206..3030
                     /locus_tag="PKL60_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW94726.1"
                     /translation="MTSPAKPIYDSAAHALPALSELRELRHYRFLLFQLIRRDLVARY
                     KRSILGVAWTMLNPLGTMLIMTLVFSNLFKSVESYPVYILSGLIVWNFFSQGTNAAMV
                     GLVWGGSLMQRIYLPRTIFGVSAIGTALVNLLIALIPLTLVMLITGTPIPLTVLFLPV
                     SILLLTAFTLGFGLMLSALAIYFPDVAEMYQILLTAWMYLTPIIYPEEIIPERFLGLY
                     RVNPMYWMVKLFRLPIYNGRIPGMAEILPALAWAVGFLVIGWLFFTSKSDEYAYRV"
     gene            3014..3799
                     /locus_tag="PKL60_00925"
     CDS             3014..3799
                     /locus_tag="PKL60_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW94727.1"
                     /translation="MLTASDLSASVLAAPSASEPIVVLEDVSVQYRVPKERIGTFKEY
                     AIRALQRRLQFMNFLALKDVNLTIYRGEVFGIIGNNGAGKSTLLKVISRVLRPTKGRV
                     ALYGKIAPLLELGAGFHPELSGRENVYLNGALLGYNQSEMDAVFESIVDFAELRDFID
                     APVRTYSSGMYARLGFAVATARVPEILIVDEILSVGDESFQRKSSDLMQSFQKQGTTV
                     LIVSHNLTKIQEMCQRAAWLSRGELKMVGDPARVVQAYRENNQ"
     gene            3801..4382
                     /gene="rfbC"
                     /locus_tag="PKL60_00930"
     CDS             3801..4382
                     /gene="rfbC"
                     /locus_tag="PKL60_00930"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNW94728.1"
                     /translation="MRFEPTALPDVLLLTPQVHGDQRGFFAETFRQDAFEAACGPYHF
                     VQDNHSGSRRGILRGLHYQLRQAQGKLVRAVAGEIFDVAVDLRCASPNFGRWAGEILS
                     AENKRQLWVPPGFAHGFYVLSEWAEVFYKATDYYAPEWERGLQWNDPAIGIDWPLPAD
                     EAPLLSAKDEHNPSLACAETYPADWTVDTAWER"
     gene            4379..5464
                     /gene="rfbB"
                     /locus_tag="PKL60_00935"
     CDS             4379..5464
                     /gene="rfbB"
                     /locus_tag="PKL60_00935"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNW94729.1"
                     /translation="MMARNVLVTGGAGFVGSNFVHYLLRTDPDVRIVTLDALTYAGSL
                     ENLKDLPDPARHSFVQGDIADRALLDRLFAEHAIDTVVHFAAETHVDRSIHDPGVFVQ
                     TNVLGTFTLLEAARKAWLVDKIVPLEQARFHHVSTDEVFGTLTREEPPFEESTPYQPR
                     SPYAASKASSDHFVRAYHITYGLPVSISNCSNNYGPRQFPEKLVPLMILNASSGKPLP
                     VYGDGMQIRDWLYVDDHCEAIWQVATRGQVGETYCVGGDNQPPNIEIVRTICQIMDEL
                     QPRSVWAPHEKLISYVADRPGHDRRYDIDIAKIKNELGWQPRHTLKQGMLDTVVWYLE
                     HPEWVQSVTHNSDYSSWVQKNYSNRKG"
     gene            5467..6342
                     /gene="rfbA"
                     /locus_tag="PKL60_00940"
     CDS             5467..6342
                     /gene="rfbA"
                     /locus_tag="PKL60_00940"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNW94730.1"
                     /translation="MKGIILAGGKGTRLYPLTLAISKQLLPVYDKPMIYYPLSMLMLA
                     GIREILVITTPEEQFLYQRLLRDGSQWGLTFTYAVQPVPRGLADAFLIGREFLAGESA
                     CLILGDNIFFGHGLPEVLRSAAGLQDGAVVFAYPVRDPERYGVVEFDGNRKAVSLEEK
                     PAHPRSNFAVPGIYFYDHHVVRYAEELIPSPRGEIEITDINRRYLEEGRLQVKVLGRG
                     VAWLDAGTHESLLQASMFVQAVEDRQGMMISCVEEIAYRMGYIDQAQLMGCAQEMAGN
                     EYGQYLLRFMKGLEA"
     gene            6339..7211
                     /gene="rfbD"
                     /locus_tag="PKL60_00945"
     CDS             6339..7211
                     /gene="rfbD"
                     /locus_tag="PKL60_00945"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNW94731.1"
                     /translation="MKKILQIGTRGQLGWELLRTCAPLGEVIALDYPEVDLADSSGLR
                     ELIRKTQPDIIINAAAYTNVDKAESEPEKVRAINAAGPGVLADEAGEIGAVLLHYSTD
                     YVFDGAKGSPYQETDQPNPLNLYGQTKLEGEQAIAASGCAYLILRSSWIYSRRSGFVA
                     KVLEWARTQEVLRIVEDQISSPTPARLIAEVTASLVSRIAEHPHAWVNERKGIYHCAC
                     KGYCSRYEWASEVIRLSSQKKFNPQLFPASTADFSTAARRPLRSCLDITKFETSFDMK
                     LPFWRNALELAMED"
     gene            7688..8674
                     /locus_tag="PKL60_00950"
     CDS             7688..8674
                     /locus_tag="PKL60_00950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW94732.1"
                     /translation="MLRKFNSNIFILPNYLDDSLWKFNAPQVEPAGEKIRIFYIGTIT
                     HVPDLEMLKPAFRALAMKYPGRLEFMFYGANLQLEENIPAVVTNCQSETFVFSDYVRI
                     ALAQKANIAIAPLEDIPYNHCKSSIKFFEYAAMGLPGVYSRVTPYTSVIEEGVNGFTA
                     ASISEWIDSLSQLIENPELREKMALSAQATVRRDWLLSDHAYLWPETYARIAELTTPN
                     QAPIAGYLPILENIGFHLDDLFQRQEKEKSIFLGRQDSLGREIQELKAQVDQLSVQWL
                     DHTKLIQALETENHLVKEARDAADLECVHYALSKSWRLTRPLRKLKKFLLGK"
     gene            complement(8984..10081)
                     /locus_tag="PKL60_00955"
     CDS             complement(8984..10081)
                     /locus_tag="PKL60_00955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW94733.1"
                     /translation="MSNNNPLVSIVMSNFNGELLIKETIESVLAQTYQNWEFIIFDDC
                     STDRSREIITSFKDPRIKPYYSDTHEHMVAGFNYGISHSSGELIARIDSDDTWEPDKL
                     QKQVEFFRNHPEYGACFTLVNFVDERGNILTERDTERVLWWDLKNKTQAEWLRYFYFF
                     GCGVSHPSAMFPRKVIEEVGSYNLAFVQVQDYDLWIRIVKHYPIHIIQERLTNYRWFT
                     TGANASAPSEKGIRRANFEFSQVFCGFFDDIPDELFIEAFGADFTRKSTTDPDDLIIE
                     RMLLLFKTVFCGVVPQLGGAQKFLSLLQDEKMRGVLRDRYGLTQMSFYEITAKPIIYE
                     ETVYKASPPAFAPPKGKSRPHFLSWLRRRFN"
     gene            complement(10081..11988)
                     /locus_tag="PKL60_00960"
     CDS             complement(10081..11988)
                     /locus_tag="PKL60_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HNW94734.1"
                     /translation="MKLDRTNPIRRIAIYFLYDKDGIVDDYIPFLLRDLVKNLEKLIF
                     VSNGPLSAESKAKLAEFHPVILERENKGLDVWAYKEAMLSVGWKKLAEYDEVVLLNYT
                     LMGPVHPFKEAFDGMAERNVHFWGLSRHYGLPFDPWKTCKYGFVPMHIQSSFMVFRSD
                     FIQSKAFQHYWETIPMVRTYEESIGYHEAIFTEDFTRMGFKSDVYVNTDDLKEYHEYP
                     LMLYPRELVEKRGCPVFKRKVFYNIYNEYIEVSPGQAARDLYQFLDQKTDYDLNLIWD
                     VLLRTAHMSDIKERLQLNYILPDQVRLPDDKPDLKAALFLHIFYPDQIDYCLRYAKNT
                     PDNMDIFISTDDAEKKEAIERVFAALPDRNVKVVMIENRGRDVSALLVGFKPYIMDYD
                     VVCTAHDKKTNYFKPFLIGESFAYHCFENVLGSKEYVQNILKTFKDNPRLGLLTPPTP
                     NFGPWSSLPGNEWLINYDATEQYARELGITVPMSNAVPPIAPLGTIFWFRTKALSLLF
                     EQNYSYGDFPVEPTRVTDGTIMHSIERVYPFAAQQAGYYSAWVMTTSYSQLDITNYHK
                     ITKDYNTAFAGRVGFENREYYLSLINQGNVTNPEKLTKNAVKTRAKKLILKIAGPRGL
                     NRVSRIWRKIK"
     gene            complement(11994..12836)
                     /locus_tag="PKL60_00965"
     CDS             complement(11994..12836)
                     /locus_tag="PKL60_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNW94735.1"
                     /translation="MTKQILVTGANGYIGQKVTRQLLDQGENVIAADLALDGIDPRAQ
                     GLQVNLFTEPEKVFELASQVDVCLHLAWRNGFNHFAESHIEDLPGHFRFISGMLQAGL
                     KHIAVQGTMHEVGYWEGKIDENTPTNPISYYGISKNALRQLTFLLSKSRNAVFQWLRA
                     YYIFGDDKRNHSIFTKILEAEERGDATFPVTTGKNAFDFIEVDELACQIARTITQSEV
                     TGIINTCSGKATTLREKVEEFIATKHLNIKPQFGVFPDRPYDSPVVYGDNTKIQRIIA
                     AAGR"
     gene            13178..14437
                     /locus_tag="PKL60_00970"
     CDS             13178..14437
                     /locus_tag="PKL60_00970"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNW94736.1"
                     /translation="MLNYGFGLLLGKTREAGSFGKKISIWIVILSNVIFLLAFRYQPF
                     ITSFLTDAVGLELPKFDLPVLLGVSFFVFSAISYQYDIYYHKIAPQKNFVDFLLYMSF
                     FPKLLQGPITRAGDFLAQITESKLNVDQLSRGIYRFTVGLAKKVIIADQLGVMVDQIF
                     ANPANANGVSVAWLGAFSYAMQIFFDFSGYTDMAIGLGQMLGFNLPENFNFPYSALSI
                     SDFWHRWHITLNSWFRDYVFYPLEFKRRKQKVFRTESNTLVVFFLTGFWHGAGWQFMV
                     WGLWHGLFASLEAFLRNKKVKVKAPALIRYLATMLIVLVGWVLFRSPDLKYGLQYLGV
                     MFGLVKPIDTGLMLSWYLSGKIAFILLLAILACIPWKHLLPKFSEKISGSALNDPVRL
                     IVFTVLLGFSLMLVISSTYSSFIYFKF"
     gene            14448..15938
                     /locus_tag="PKL60_00975"
     CDS             14448..15938
                     /locus_tag="PKL60_00975"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94737.1"
                     /translation="MKTVPCRFLLIVIFLAALLLPLVLADKVGGALSPTENRNLARFP
                     QILTQEKKIAPGIKIGFEAWLKDNLFGRMEAQKARAYIDFRVLRTSPSKLVQIGENGW
                     YFYNNDQNLEIGRGAKLLSPEELEAIRMNQLAIQKALKAQGIEYILVFIPSKASVYPE
                     YMQGGKYRVGLTLIDQVTAYLQQNTTIPVINLKPDLLAAKAEQDVYFRSDTHWNQAGA
                     YIGYRGVINRLNEWKMIQSQPVSISTEPALHTGEFSYIMGFPGLISPEPIRATIIQNP
                     KASLVSSSDKTELVSRLLAETGIKGEYFSYTNPDAERKALILGDSFFFTWKIPDLFAE
                     NFAEMNFIRTDVLTSDIIQAVNPDIVIMERAERYIYNLANSSNVNLLMPRLSNPSAEI
                     ISHDTPTTIERDKSYDVNIVVKNTGDEAWSEEKHVRLGIFQDGKDYGYRIYLPEGVTV
                     EPGQEYTFVLSNFRAPEGNTTYLEYQMLQEAYQYFGEKERVDLVVK"
     gene            15973..17592
                     /locus_tag="PKL60_00980"
     CDS             15973..17592
                     /locus_tag="PKL60_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNW94738.1"
                     /translation="MTVPSFPNNNIGYLCTDWQVERGLGQKTALMFLDRYETLQELSF
                     AELTILTNKAANLLSTLGFRKGDRVMLLLSKIPEMFAFFLGGMKLGLNCCILFTSIGE
                     DSIIERIRDTHTRAIISTSRMNFRLEKVVQNLDEDFRILLVDQSRAEGRFVGIQQGFE
                     TASADFSTPDTNPEQYSHFHFTSGSTGKPKAVQHCHGAIIDHLRSFEAVMQADPDDLY
                     WCTADPGWVTGTTYGITAPWASGLTQIQLDANYDPELWMRIIEHQRVNVFYTAPTVFR
                     MMAQQTDAFYQQYNLRSLKRVYTVGEPLDPNLVLWGRKVFQCEIYDTWFQTETGSIMV
                     ANRPGLEVRPGSMGKPLPYLEAAILESKDHPLPENTQGLLCIRKPWKSMFREYVGSPL
                     VYQNKFLGDFYNSGDIAYRDEDGYFWYIGRSDDVINTAGHLVSPFEIESAVMEVPHVL
                     DCAVVGLPDELLYEKVVAFVVHDQQALSLRKLDMEIKLFVSKKVASLAAPKEIVFIEK
                     IPKTKSGKVMRRVLKKQYLGEDVGDLSTMEQ"
     gene            17594..17839
                     /locus_tag="PKL60_00985"
     CDS             17594..17839
                     /locus_tag="PKL60_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNW94739.1"
                     /translation="METAEMKSRMNKVFREVFDNPGIEIFDGMTSSDVVGWDSFSHIN
                     LITSLEIEFDIEFSQAEAFGFKTVGELQKAILRKMSA"
     gene            complement(18008..19486)
                     /locus_tag="PKL60_00990"
     CDS             complement(18008..19486)
                     /locus_tag="PKL60_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNW94740.1"
                     /translation="MPRRKVFRLLLVPLLLLSFAWLPAQKAAAKAEPMPLADVSAGDL
                     IAAMNTLRMANGLTALIEDPIIDAVAQSTAQIMADQLLSWHIGDVKGRIAAAGYGGGG
                     TVWATENFAIGYNAGIDEIMLMWADPDHMRPATQSYYCNVGAGVARASNGSTYYILQA
                     AYVSGKECGTYTSPGGPVDGGGGYGIIVPVALAEPDASGRIVHEVQSGQSFWAIAVAY
                     QVTIKEILLWNNLPEGATLQVGQLLVIPNPNAPGYQTPTPVGYFSANPPGPDGKITHT
                     VGAYQNLTLISQVYGVSIERILALNSLTIDMPLQIGQILLIDPGNVTPTPTPRPLSPL
                     EMLTPAADGKYYHTVGEGQNFSWIARYYGIALEDLLRWNNLTVNYTLWAGEKLLLNVT
                     PPATMTPTPPPVTPTHLPTPTTQAAPTRTPLPSPTPTATAAAGVWEQISGARPLWLIA
                     AAALLAGAVGLLLSAEHKRKAAGKDQAGEPKEEASDRHSRDG"
     gene            19661..20683
                     /locus_tag="PKL60_00995"
     CDS             19661..20683
                     /locus_tag="PKL60_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW94741.1"
                     /translation="MKKLVIQIPAYNEAENIAGTLADIPRRYEGIDEVLVVVIDDGST
                     DGTASAALENGADAVIRHRRNRGLSRAFISGIQFALALGADIIVNTDADNQYVGGEIQ
                     RLIQPILDDSADLVIGDRNPAQNAHFSPLKRRMEAFGSWIARKVSGTNVPDTVSGFRA
                     YSRYAALPLQVFNPYSYTLETLVQAGKSQMKIAHIPIKTNPNLRESRLHKGLLHFIWK
                     QSGVIVRSYVLYQPLRTFVSLGAVFLAAGLALLLRFLIYYFITNTAGRLLQSVTIGGM
                     LTIVGIILIIIGFIGDSVRASRQISEEILTSLRDKTRLGQLSDVREFNGQPVFTPEHP
                     VGTGQL"
     gene            22141..25965
                     /locus_tag="PKL60_01000"
     CDS             22141..25965
                     /locus_tag="PKL60_01000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNW94742.1"
                     /translation="MSFVHLHVHSSYSVLDGFGKPADLVARARELGMPAVALTDHGTM
                     FGTLDFFKEANAAGIKPIIGLETYLAPRSRFDKDVSKDKKPFHLVLLAENMQGYKNLL
                     QIATASQLEGFYYHPRIDKEYLAAHADGLIASSACLSGELPRALLEGDPEKAERALGW
                     YLDLFGRERFFLEIQDHNLPELNQVNRGLLGLADKFQARMIATNDVHYIRREDAEMQD
                     VLLAIQTGKLLTDQNRMTMTDDSYYLRSPQEMRYLFSEIPEALSNTLEIAARCEVDLT
                     RKGYHLPRFELPNGEEPGAYLRELCVEGLRKRIPAEADLPETRERLDYELSVIQKMGF
                     DEYFLIVWDLCRHAREKNIWYNARGSSAGSLVAFALEITSVEPIRHKLMFERFLNPDR
                     VTMPDIDLDFQDDRRADMMEYCNEKYGADKVAQIITFGTMGARGAIRDVGRVMNIPLP
                     EVDRVAKIIPGPQQGKTPSIAETLEKSSELRAVYESSDQMKKLIDTASRMEGGIRSVG
                     THAAGVIISDEPITEYLPLHRPTGQNEDLPIKSVAQYDMDGINDLGLLKVDFLGLVTL
                     TIMAKACELIERRHGRKLTLENIPTGDPEVYRYISEGHTVGMFQLESSGMTSRIMQMQ
                     PTELAHLIAMIALFRPGPMDSIPEYIDRMHGRKEVKYAHEKLKPILEETYGHAVYQEQ
                     VMQAAIALAGYSPADSDKLRSAISKKKEKEIAAHRVQFIEGAVKNGLTRKEAESIFAD
                     WEGFAHYGFNKSHAADYGVIAVQTAYLKYYYPVEYMTALLSAWKNDIEKCALYVAESK
                     AMGLEVLPPDVNTSGYDFVIEDRPDQSAAIRFGLGAVKNVGQAPVDLIIAARGGRNFT
                     NITDFARRVDLRQVGKRPLECLIKVGALDSFGPRHSLLSSLEQLVSVSAAHFRAVELG
                     QLTLFSGAQEPGAEVRLFAGSAADHNEQLEWEKELLGLYVSDHPLNTYMSLINGQITH
                     LANQLEELNNKEAVVVGGLVKKARPILTKKQQEMGFVTIEDNTGEIELVIFPSVWETS
                     ASQIEGGALLLVKGKVDHRESSVSILADQVTRVETSSLKDPLTDNRNQGPYYEQVLAK
                     YLPDISALRPYAFDAGADRNSEPDAAPRSTEAAQPADPFEEPEWEESAFSSFPPPAAP
                     EPEAELDLPAAEADPPAHAEAEGAPAQPFSAALPSDAPAQTEVVRSPRLLVTIQSCGE
                     KERDLRQLKQIHGFLTSHPGENRFFFYIAEAGATYEIEFPNETTDINERILTELNRFL
                     GGTNVQLAVDGN"
     gene            25982..26506
                     /locus_tag="PKL60_01005"
     CDS             25982..26506
                     /locus_tag="PKL60_01005"
                     /inference="COORDINATES: protein motif:HMM:NF022734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HNW94743.1"
                     /translation="MRDVDLDFNPTEVLILSAIGKPGERTFFIRGGNKSLTITLRMEK
                     IQVQMLAAGVVHFLEELKTNFPELDEPAGTYDESRMVLKPPIDPLFQVAEMSMGYDRG
                     NDLFVFIFESPKPEEAAEAPGVVRFWCKRAEAAGLAAWARELISRGRSTLAEPGSAGE
                     PDGEISPRNNGHKH"
     gene            26515..27237
                     /locus_tag="PKL60_01010"
     CDS             26515..27237
                     /locus_tag="PKL60_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HNW94744.1"
                     /translation="MDEPTQLRVLQHGQVSLLGQFTRSSNFTFLVECTLEAHSLRAVY
                     KPARGQQPLWDFAAETLPKRETAAYLLCRAIGWDFVPPTVYRTEDLPAGEGSLQWFVE
                     HDPEQHYFTLTEAERASLQPLALFDLLANNADRKGGHILRDEAGKLWAIDHALCFHAE
                     TKLRTVIWDFAGQPLGEALLNSLDALNLRLSPGQPFLREISRYLSAAESDSLRERCQK
                     LLADGHFPFPAEDRRAFPWPPV"
     gene            27289..28323
                     /locus_tag="PKL60_01015"
     CDS             27289..28323
                     /locus_tag="PKL60_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNW94745.1"
                     /translation="MTETVRLVMVGFGNVGKAFARLLLNKDAELLERYQLKLLVTGIC
                     TRSHGSAIDPRGLDLQAVLEALKTGGSLSGLDTAGFSGSALDFIGACEGDVLLESTPV
                     NPKSGQPAIAHLEAGLRKGMHAVTPNKGPVVFGYQHLTALAESKGKKFLFESTVMDGA
                     PIFSLFRKNMALAELKGFTGVLNSCTNLLIGRMEDGESFAQALDYARSIGITETDPSN
                     DVDGWDAAIKVSALATVLMKHPLTPAEVQPTGIRGITPEKIAEARAAGKRWKLVCSAG
                     YDEQGQFFARVCPEMVGPESPFYSVWGTDSIVVFNTDVLPGLGVLETKTTPYTTAYGL
                     LSDVLSALRD"
     gene            28334..28810
                     /gene="folK"
                     /locus_tag="PKL60_01020"
     CDS             28334..28810
                     /gene="folK"
                     /locus_tag="PKL60_01020"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNW94746.1"
                     /translation="MAVVFLGLGTNLGSRAENLSAAREQLAAQMRLLRASSVYETEPW
                     GFREQPAFLNQVVQAETDLSPLKLLRFLKRLEKQLGRTETFRYGPRLIDMDILFYDRA
                     VINTRRLQIPHPRLPERAFVLVPLAEIAPDFVHPVLERTMRELLRDCPDQESVRKC"
     gene            28804..30645
                     /locus_tag="PKL60_01025"
     CDS             28804..30645
                     /locus_tag="PKL60_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94747.1"
                     /translation="MLSALALAAFLLPGLCWWVWLGDRDKDPLEALAGILGVSVALTA
                     LGALFFYTVRLSVSAGLLGALLGLTLSLTGYGVYRRRKEGVSPWGWLLALAVFGALCV
                     WRLWQARGLALPAWVDSLHHSLIIRKMIEAGGLSSTLEPYLPGPFYYHYAVHSAAALV
                     SALSGDAPAQTLLWLGQIFTAGVSLSAYSLVKASTRDWRPAALAGLLVTFATKMPGYY
                     LSWGRYTLLTGMLMLPLAMAEALRVARGERLRRSAPLLVVLTAGTLLSHYLAAFLLAL
                     FLIVLALAQLGRNIRPKNRDWRPLLALTASALAGLALVSRWYWRVFRYSRGMMSTEIQ
                     LPASGALSAEHWDYFWNLVGPLTGYVVLGLACAGLVWAFTRPKTRPLALWSLLVGLLS
                     LPFGLVLGPFRSDHFALALFLPAACLAACLLVWAGDWLRSRHPRQVWGTAALLLVCAA
                     LLGGGAWLNREPVNASTVLADESDLKALEWVEDHLPSDARFFINTTGWGYGLYRGMDG
                     GAWVLPYTGRWSLAPTIFYTFSGEEQSYGAWIDWSQRANVVTGCTEEFKALMTEAGLS
                     YLYLREGVGSLQANALRGCPEAKQLYSTGGVSIWLWDDASIDAGQGE"
     gene            30845..32704
                     /locus_tag="PKL60_01030"
     CDS             30845..32704
                     /locus_tag="PKL60_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94748.1"
                     /translation="MLLTLSIWQWFKLAASFFLFFMPGFLLIGAFWGKRTISFGQKTS
                     LAFGLSLGVWCVLLVWLDFLNLSLNTGLVRMFFGLCLAFVILQIAATRTLPYFPRAGI
                     QGLEMFITVLSALLILFFAYLLRDLASGMGSDSMHHTLIPMLMLKYGQIPSNYDLFAP
                     VSTFTYHFGYHAFVSAISWLSGVSPQIVVILSGAFFVGLCAFIAAVMIETVTKNKLMA
                     LCAVPLIGLIYIFPSYSLMWGRYTQFLATMLLMVLITVILVSEETSGFSRKDIPLFVL
                     LTAGLFFSHYRIFLAACLFLGCYFLVRLVKIKEKKEYFWTWVFIGLGTAVVSLPWIIR
                     LLDVRFGSLGNRVLISDPTYFSLARLGPLFAGEFQNIFLIILAGVSGLFGIARKNTVA
                     LSAMIWVALIWVLSLPGKIGSLLDPVTMMISSYLPVVLLLLIGVDHAYRMIKNRRIGL
                     LVNLLLVCVVVYAAGIGIVRWVNIDFEPEEFVTFEDVQAGKWIEQNTPSDALFVTNTY
                     QFSFQEELVTAMDAGYWLPVIAHRPTLIPPMVYLIERMNAEDVQYRGAIVHSFGGHLG
                     SQEAVKYLLSQGVDYLYLGAKGGIINSSEIDASMDYALVYNTQSVRVYKLLEH"
     gene            32892..34064
                     /locus_tag="PKL60_01035"
     CDS             32892..34064
                     /locus_tag="PKL60_01035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW94749.1"
                     /translation="MKILIATTNFPRWQGDFRVPFIYEAAKALQAKGNTVRVVAMHNP
                     GSREHELMEGLEVFRVRYLPEEKEKLQQDAAGIPAAWERGLKGRLALIPFLVAYTKAV
                     GRHAADCDIIHANWSLSGLAALLSRGRHKLPYVVTVQGSDVFKTLNKPGVRQMVGLAL
                     RKAGKVIALSRALKSAALEFGLQEEHVVVIPNGINISQFPFATLDEKKNQVLFVGSLI
                     ARKGPATLLQAMRLVHQDLPDTKLVLVGEGDERAHLEKFIAEHDMADYVCLAGTQTQS
                     AVAELMRASRLFVLPSIEEGQGVVLMEALASGTPCVGSNVGGIPDVVTEDVGRLVEPG
                     DAHALAEAIRAYLTDAELWQKASAAGRQRVLEHYDWNHLAGEISAVYAEVLAERST"
     gene            34061..34849
                     /locus_tag="PKL60_01040"
     CDS             34061..34849
                     /locus_tag="PKL60_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNW94750.1"
                     /translation="MREYQKDFITLYDDFQDGENRKRKAEKINTILEKLKLHNPQGTC
                     LDTGCSNGIITRSMAELFAAVVGVDIDRTAMKLVDPRENPAVSYVYGDAMGLPFADEA
                     FEALVCSQTYEHVPSSPHLFAEILRVMKPGGVVFFSGPNKVFPIEPHYYLPFLHWLPE
                     KWADGYLRLTKRGTHYYERSRTYWDLRRTFLKDYEVHDAMRYVLQYYALTNPKKGMRR
                     LYSLAAKLPPFLLKLLSPFMVNINWVLVKREAGKNGAKNIQFAR"
     gene            34818..35552
                     /locus_tag="PKL60_01045"
     CDS             34818..35552
                     /locus_tag="PKL60_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNW94751.1"
                     /translation="MAQKTSNSRVRRRRQEIERELTRSGPDAWKYFSTVIYSHWRIVI
                     EGCLPKYLQGLALDVGSGACPYRALAESHASRLLLMDHNAQPIRIDLRGDIQNIPLRA
                     EAVDSVLCFQVLEHVANPFEGIAEIGRVLRPGGILLLSVPHLSRLHELPHDYYRYTEY
                     GLRELAKAGGFAVLELEPVGGLVSFLGHQLSMAYLTLFWPLRFLRPLTLFINKNFFTR
                     FLTFVDRYLGFKTLFPQGYALVARKL"
     gene            35549..36499
                     /locus_tag="PKL60_01050"
     CDS             35549..36499
                     /locus_tag="PKL60_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94752.1"
                     /translation="MKNWPQIRRILRIAGTLIGLVIFAYQLYTSIGSLSTQKQQLQLI
                     WGWIFLALALYLLVYLLQMVNLWLVYKTNFPAIKLKPIVQGYAMSFLPKYTPGYIWGY
                     LSRADWLESRAGIPGAYSWLATVLEVVVTLMSGAVVLLLNWAARGGAYLALAVLAGLL
                     PWLVWLLMRLVARFARKFLKEERQRVLEGFAIPFARWALISVNSVVQWLLLGVGLRLI
                     SAGFSYQTPLPFWSALWKHIYAFCISWLGGFLAVLIPNGLGVRETVLNALLESQVGFA
                     AAAAVVVSVASRVLLFLAELVWLLHALVTKQDSPADLKYS"
     gene            36567..38000
                     /locus_tag="PKL60_01055"
     CDS             36567..38000
                     /locus_tag="PKL60_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94753.1"
                     /translation="MKKYFSVVLVCLLVFLLGVAPVSQSHQMAIAAATTKSFSSNYTL
                     VNLGGDTAAVTASYIAPNGTEWGSSIFKSFSIPAGANQIVRQYDDTGLTPGRGSVVIA
                     SSQPLGALVQEIVRTGVPSSGAYTGIDVPSSIWYVPEVSRMGNSATGTANSQIVVQNV
                     GTAAVDFTIALTAFGAATPTYTKSFTGLAAGASILYDIQEETNLPTGFWGSAVVSTAS
                     GSLGVVSHLFFGADSLMAFNGFAAADDSWYVPLLYVRLGNSLTTSLAIQNLSGDTIPV
                     NDLTLACTKNPAAPGAATITLHNHTAIANNGAYTFNTLTDTTRFPDAGWYGSCKISSA
                     TAKDTVTLVMYRYVANAEQAAYGAVPGSLASTKVSVPLVAKRLANGYANVVTVQNLST
                     SAANVTITYTPAGGGTPIVRTGLTIAAGDSLIRNFRLSGTESPDISDGWVGSMSVSAD
                     QPIAAYVANTYLNPSGDQFMAYLGFNY"
     gene            38067..38660
                     /locus_tag="PKL60_01060"
     CDS             38067..38660
                     /locus_tag="PKL60_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94754.1"
                     /translation="MKKTFLPLLLVLSLILAACSSNTPAPTQPEASPSTALPEVTQSS
                     PETAYPAAEMRLSSAYPIKLPEGVPLSSLSAPTEALPPQEGFASISGLLYAYDISQIL
                     VDTDFWLFPAVTVGDQKVPPPIITNGDPAAGDIYGRTDANGAFYLDNVPAGDYFLLIN
                     YPDHTVFGQSSPTPDAYLLISVAAGDRIAYGVIYTLR"
     gene            complement(38664..39962)
                     /locus_tag="PKL60_01065"
     CDS             complement(38664..39962)
                     /locus_tag="PKL60_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNW94755.1"
                     /translation="MEKLPLSDREIEILQLVGQGKSNKDIAAELFISVNTVKVHLGNI
                     FAKIGVASRTEATLYAIERGIVKSPAGANGEVEVLVPEPIQAPEAEPTRLQAFLRKYW
                     PGLALLALAAVIGLSFALASTPLFSPPTATPNPVLSALMEQRWQVMASIPTETAGAVL
                     INAGDKLFAIGGLSAEGVSGRVDIYDPQQDAWSAGEPKPLPAADVSAALLDGKIYVPG
                     GRLASGELSSALEVYDPLSNRWERKADLPYPVSAYALGVSGGSLYLFGGWDGSQTLDA
                     VLRYSPAEDRWTTAGSMPGARESMGSALVGGRLYLFGGTNGSKTLDALWTTPLGGELA
                     WQAEAKTPFKCARCTGAALNDFIFAVSESTIWQYNPKDGSWLSDPGMPVSSPRTDQSL
                     TGYGNALYILGGMDETGSLSAELIRYQAIYSLIIPFLTNP"
     gene            complement(40070..41176)
                     /locus_tag="PKL60_01070"
     CDS             complement(40070..41176)
                     /locus_tag="PKL60_01070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNW94756.1"
                     /translation="MQNQTFTPATDPEERPKKSYKFLFIAAALLFVLSLVLIFSDGNK
                     SGSKNPPGEGTALDSLLFAQLPGALAERGLDGLKLDDITFNEVKDQAVLWLTASDPDT
                     GELLAREPEVILALWNEDTGQWNLYQKDDDVFLDKLNESDFRDSEIYGRFTEYDPKAS
                     VTGTIYGGFKLPWRGGLTKRLTWSVGHNSCANNYCLYAFDFADGTMFEITAAKSGYVY
                     HFKDSCNNGDSTCTNSITIQDRSTSPYTYHIYMHIAKGSVPENLRKFGVPVFQGQTIA
                     LADDTGYSTGHHLHFMVVEQSTLNACTNYCWGKSVDITFRDVDINWDAATQGGRPRLA
                     SEASWYGGTGRTYYTSGNPYTPASHTYNIILLYK"
     gene            41920..42834
                     /locus_tag="PKL60_01075"
     CDS             41920..42834
                     /locus_tag="PKL60_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94757.1"
                     /translation="MMNQKIDRLSEVVLREIGGRMVGKRLVPLLRLLFSRQAREFSRL
                     LLALEDGLDCPQPWLTARATASALSGGLEIRAQERVPAEGPLLALANHPGMADAIAAF
                     GCLPRADTLVVAGRTPILTGLPNISRRLIYLPENKAGGMVVLRAMVDWLRKGGAVFYF
                     PYGLLEPDPARVPGAANTLQAWSGSIGAVLSRAPETKLLPMLISGVLSKRAWESGLLS
                     QVVKTKRRHQLAMIWQFIRRVGAHGRDWDSVMRVDIGQSLPARSLSARLDPAEINAAA
                     RAAFGGLLTDTFPEDPGMLPVCDYVTPA"
     gene            complement(42831..44039)
                     /locus_tag="PKL60_01080"
     CDS             complement(42831..44039)
                     /locus_tag="PKL60_01080"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNW94758.1"
                     /translation="MNEAVIVQALRTPIGNLNGALSTVRPDDLAALVIAEVVRSTGID
                     PHEIDEVIFGCANQAGEDNRNVARMAALLAGLPPEVPALTVNRLCASGLAAVNLAARA
                     IRAGDGEIFVAGGVESMSRAPYAFAKTLSGGNLTAYDTALGWRFINPRMEALYGTEAM
                     GLTAENIAAQTGITRQEQDEFAFLSHQRAVAAQDQGKFAEEILPVTIPQRKGEPLLVS
                     LDERPRRDTSLEALGKLKPAFKPDGSVTAGNSSGLNDGAAALVLMSAEKARALGIKPL
                     ARVVSGAASGIEPRVMGLGPIEATRRALKKAGLTIEDISLAEINEAFAVQALAVMREL
                     GLNHALTNVNGGAVALGHPLGCSGARILTTLVHEMRRRAPEAKRPFYGLAALCVGVGQ
                     GEAALVEWLD"
     gene            complement(44219..>44849)
                     /locus_tag="PKL60_01085"
     CDS             complement(44219..>44849)
                     /locus_tag="PKL60_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94759.1"
                     /translation="LSNHTWLMEGVVLNLLPYESKVIDFGEIKEQSDEISTPGRISFT
                     EDCLVWKVEGQEIQLAGLSDLAIGQRIKFNAEYIRESHPFQAQATEVLILTPGAVESS
                     TPISTFLSPEFTGTITSIEEYVHINTTKLIDLHVTSSPKYPDLNETRLALAYYTLLWQ
                     QTATGYLSVTEDALKPGQLVSVLLTDHWSKDIYDYAAFQGIVQEIIIIP"
BASE COUNT         9521 a        12352 c        12734 g        10242 t
ORIGIN      
        1 aaacacaaaa aattcttttt agggcttatt cctgtattgc tgttccgcac ttctttccat
       61 cttgagcaag agcgcgtaat taatcgcgcc gacgatgacc attacgatga gcaaaaccag
      121 gtaagccgtc tgccaattcg tggggccgac catcatcgtg aattccgaca tgccgccaat
      181 gcccgaaatt aatgtgagcg gcataaaaat ggtcgttatc agggtcaatc ggcgcataat
      241 cgcgttggtg cggttcgaag actctgccat ggcattgctg atgagcgaca ggtggatttc
      301 catcaggttg gtgacctgtt cgcgggcggt ttccgcaatt tcgtagtact ttgacaagtg
      361 gtcgtaaaca tcccggaaat aaatcaacga tttttcggcc aggaaggggc tgtctttgcg
      421 cacgagcttg ccgaccagtt cgcgctcgtg aaagagcgac ttgcggatga cctgcaggtc
      481 gcggcgggaa tccatcaacg cccccaaatt gtagctggca gcatccctga gtatcgcgtc
      541 ctcgtcggaa tccagctttt cctcaatgct ctccaccgcc agcagtttat ggtccacaat
      601 catatccacg acctgatgca gcaaatacga agggcccaga cggacggcat ccatctcccg
      661 cgtgacagag gcttccatcc cggccaagac cgaatttccg ttcaaatccc gactgcttac
      721 ggtgataaag tattgcttgc tgatgaataa atccatttca tgcgcatgca gcccatcagt
      781 gtctcgttca aagatggaaa aggtcatgaa tgaataagtc gggaagaggt ccagcttggg
      841 cagttggtct ccattcaggc agtcttcgat cgaaagcgga tggatgccga gttttgtctc
      901 cagttcggat agcgattccg cgtttgggtc gatgtaatca caccagacat agccttctct
      961 gcctgcaaac agcagtgcgt cgtccagccc ggatgcccgg tgcagcgtcc ccgactttgt
     1021 gatggcgaag taacggtttt cgggtgtgtt cataaactta atccttccag ttctatcgcc
     1081 gcccgtcaac ttgccgccgg cgggtgcttg cttggcccgc tcaagcgtct acctgcagag
     1141 cagctttacc agcggggcga acgggttcca gtttaatagg cgggcaaatc gcgcggctga
     1201 aacgcgcctg ctgccctgaa ctttccttcg cttctgccag actgctggca gcgttttcag
     1261 cgactcccat ttggcgcgag cgataagcct gccgcgcccg gtgagcgcgg cagccagcag
     1321 gtatgccagg ttcaccagca ggtgcgcggg taataagagc cagaaaaacg cgccaggcat
     1381 gttcttgaca taagtccaga taagattgcg gtgcgaatac tgcagcgaaa aatcgctccg
     1441 tacagcggtg ctgcccgcgc ctatatgccg gacgaccgcg cgcgggaggt agtaacacgg
     1501 atacccgagc aagcgcagcc tcaggtccag gtccacatcc tccaggtagg caaagaaatc
     1561 ctcgtcaaag ccgccggctt cccggaaagc agccagagga taggcgctgg cagcggcgca
     1621 ggcgctgatg acttcgcgtt cagacgggca ggctctcgcc agcggctgcc cgtgggattt
     1681 gcgccacgcc aggcccgctg cggaatacac attccactcc ccatccaggc ggtctgggtc
     1741 cccggccgtt aattggcgtg aggcaaagca agcgcccggg cgcttttgcg cggcagcatg
     1801 gatttccgcc agccattccg gccgcgggaa ggcgtccgcg ttcagcagca ccaggtactc
     1861 cccgcgggcg tacttggctg ccaggttgtt gccgcctgca aaaccgaggt tgcgttccga
     1921 acgcaagact gtgagcggaa gccttgcatg ctgcgcgagc atatttgcat cgagagcgtc
     1981 cggcgagccg ttgtccagca ggataagctc aaaatcccgg taagtttgct cttccagcgc
     2041 gtccagcgcg gcggcaagga agctcccgct gttccagtgg agcatcacaa cggaaaagaa
     2101 gggcacgggc tcggcggcga gggccgcggg ggaggtaagc ggtttctcca taggctatgg
     2161 tagtattata atttaggaac actcgaggca accaaggatt ctgtattgac cagtcccgcc
     2221 aaaccaatct acgattctgc cgcgcatgcg ctgcccgcgc tttctgagct gcgagaactc
     2281 cggcattacc gttttttgtt attccagctc atccggcgcg atttggtagc gcgttataag
     2341 cgctccattc tgggggtggc gtggacgatg ctgaacccgc tgggcacgat gctgattatg
     2401 acgctcgtgt tttccaacct gttcaaatcg gttgaatcct atccggtcta tatcctcagc
     2461 ggtctgattg tgtggaactt cttctcgcag gggaccaacg ccgccatggt cgggctggtt
     2521 tgggggggca gcctgatgca gcgcatttac ctgccgcgca ccattttcgg cgtgtccgcc
     2581 atcggcacgg cgctggtgaa cctgctcatc gcgctcatcc cgcttacgct ggtcatgctg
     2641 attaccggca cgcccatccc cctgaccgtg ctgtttctgc cggtgtccat cctgctgctt
     2701 acggcgttca cgctcggttt cgggctcatg ctctccgccc tggcgattta cttcccggat
     2761 gtggccgaga tgtaccagat tctgctgacc gcctggatgt acctgacccc catcatctac
     2821 cctgaggaga ttatccccga acgcttcctg ggtttgtatc gcgtcaaccc catgtattgg
     2881 atggtgaagc tcttccgcct gcctatctac aacggccgca tcccgggtat ggctgaaatt
     2941 ctgcccgcgc ttgcctgggc agttggtttt ttggtcattg gctggctctt cttcacctca
     3001 aaatcggatg aatatgctta ccgcgtctga cctttctgca agcgtgttgg ctgccccctc
     3061 cgcatccgag ccgattgtgg tgctcgagga tgtcagcgtg cagtaccgcg tacccaagga
     3121 gcgcatcggc accttcaagg agtacgccat tcgcgcgctg cagcgccgcc tgcagttcat
     3181 gaattttctg gcgctaaaag acgttaacct gaccatctac cggggcgagg ttttcgggat
     3241 catcggcaac aacggcgcgg gcaagagcac cctgcttaag gtcatctcgc gcgtgctgcg
     3301 ccccaccaag ggtcgcgtgg cgctgtacgg caaaatcgct ccgctgctgg agctgggcgc
     3361 aggcttccac cccgagcttt ccgggcggga gaacgtgtat ctgaacggcg cgctgctggg
     3421 atataaccag tctgagatgg acgccgtgtt tgagagcatt gttgatttcg cggagctgcg
     3481 cgattttatc gacgcgccgg tgcgcactta ctcctcgggg atgtacgccc gtctgggctt
     3541 tgccgtggcg accgcgcgcg tgccggaaat cctgattgtg gacgaaatcc tgagcgtcgg
     3601 ggatgaatcc tttcaaagga aaagcagtga cctgatgcaa agcttccaaa aacaaggcac
     3661 cacggtcttg attgtctcgc acaacctgac caaaattcag gagatgtgcc agcgggcggc
     3721 ctggctgagc cgcggcgagc tcaaaatggt aggcgaccct gcccgggtgg tgcaggctta
     3781 ccgggagaat aaccaataac atgcgcttcg aacccaccgc cctgcctgat gttttgctgc
     3841 tgacccccca ggttcacggg gaccaacgcg gtttctttgc cgaaaccttc cggcaggacg
     3901 cgtttgaggc ggcttgcgga ccctatcatt ttgtgcagga caatcactcc ggttcgcggc
     3961 gcggcatcct gcgcgggctg cactaccagc tccggcaggc gcaaggcaag ctggtgcgcg
     4021 cggttgcggg cgaaatcttt gacgtggcgg tggacctgcg ctgcgcttcc cccaacttcg
     4081 ggcgttgggc gggagaaatc ttgagtgccg aaaataagcg gcagttatgg gtgccgcctg
     4141 gcttcgcgca cggcttttac gtgctcagcg aatgggcgga agtgttctat aaagccaccg
     4201 attattacgc tcccgaatgg gaacgcggct tgcagtggaa cgacccggcg attggcatcg
     4261 actggccgct gcccgcggac gaggccccgc tgctttctgc caaagacgaa cataatccaa
     4321 gcctggcctg cgcggaaact taccccgctg actggactgt agacacagct tgggagagat
     4381 gatggcgcgc aatgtgttgg tgactggagg ggccggcttt gtcgggtcca attttgtgca
     4441 ttatctgctg cggacagacc cggatgtccg gattgtgacc ctggacgcgc tcacctatgc
     4501 cggcagcctg gaaaacctga aagacttgcc cgaccccgcg cgccacagct ttgtgcaggg
     4561 cgacattgcc gaccgcgccc tgctggaccg gcttttcgcg gaacatgcga ttgacactgt
     4621 tgtgcatttt gccgccgaaa cgcacgtgga ccgttccatt cacgacccgg gcgtgttcgt
     4681 gcagaccaac gtgctgggca ccttcacgct gctggaggcc gcgcgcaaag cctggctggt
     4741 ggataaaatc gtgccgctgg agcaggcgcg ctttcaccac gtgtccacgg atgaagtttt
     4801 cggcaccttg acgcgcgaag aaccaccttt tgaggagagc actccctacc agccgcgctc
     4861 tccttatgcc gcctccaaag cttccagcga ccacttcgtg cgggcgtatc acatcaccta
     4921 cggactgccc gtcagcatca gcaactgctc caacaactac ggtccgcgcc agttccccga
     4981 aaaattggtc ccgctgatga ttttgaatgc cagctccggc aaacccctgc cggtctacgg
     5041 cgatggaatg caaatccgcg actggctgta tgtggatgac cactgcgaag ccatctggca
     5101 ggtcgccacg cgcggtcagg ttggcgaaac ctactgcgtg ggaggcgata accagccgcc
     5161 caatatcgaa atcgtgcgca ccatctgcca aattatggac gaactgcagc cgcgttccgt
     5221 ttgggcgccg catgagaagt taatcagcta tgtggcagac cgcccggggc acgaccggcg
     5281 ctacgacatc gatatcgcca aaatcaagaa cgaacttggc tggcagccca ggcacacgct
     5341 caagcagggc atgctggata ccgtcgtctg gtatctggaa cacccggagt gggtccagag
     5401 cgtcacccat aattcggatt acagcagttg ggtccagaag aactacagca acaggaaggg
     5461 ttgaatatga aaggaatcat tctggcaggc ggaaaaggca cccggcttta ccccctcacc
     5521 ctggcaatca gcaaacaatt gctgcctgtg tatgataaac cgatgattta ttatcccttg
     5581 tccatgctca tgctggccgg catccgggaa attctggtca ttaccacgcc ggaagagcag
     5641 ttcctgtatc agcgcttgct gcgggacggc tcccagtggg ggctgacgtt cacctacgcc
     5701 gtgcagcctg ttccgcgcgg tctggcggat gccttcctga ttggcaggga gttcctggcg
     5761 ggtgagagcg cctgcctgat tctgggcgat aacatcttct tcgggcacgg cctgccggaa
     5821 gtgctgcgca gcgcggcagg actgcaggac ggcgcggtgg tgttcgcgta tcctgtgcgc
     5881 gatcccgagc gctacggcgt tgtggaattt gacgggaacc gtaaagccgt cagcctggaa
     5941 gaaaaaccgg cgcatccgcg ctcgaacttt gccgtgccgg gcatttactt ctatgaccat
     6001 catgtggttc gctacgcgga agagttgata ccctccccgc gcggcgaaat tgagattacg
     6061 gatatcaacc gccgctacct ggaggaaggc aggctgcagg tgaaggtgct gggcaggggc
     6121 gtggcctggc tggacgccgg cacgcatgag agcctgctgc aggcttccat gttcgtgcag
     6181 gcggtggaag accggcaggg gatgatgatt tcgtgcgtgg aagagattgc ctaccgcatg
     6241 ggctatatcg atcaggccca attgatggga tgcgcgcagg aaatggctgg gaatgagtac
     6301 gggcagtacc tgctgcgctt tatgaagggg cttgaggcgt gaaaaagatt ctgcaaattg
     6361 gtaccagagg tcaattaggg tgggaattgc tgcgcacttg cgcgcccttg ggcgaggtga
     6421 tcgcgctgga ttatcccgaa gtcgatcttg cagacagcag cggcttacgg gagctcattc
     6481 ggaaaactca acccgacatc atcatcaatg cggctgccta cacaaatgtc gataaagctg
     6541 agagtgagcc cgaaaaagtc agggcgataa acgcggctgg acccggcgtt ttagccgacg
     6601 aagccgggga aatcggcgcg gtgctgttac attattccac ggattatgtg ttcgacggcg
     6661 ccaaaggcag cccttatcag gagacggacc agcccaatcc gcttaatctc tacggtcaaa
     6721 cgaaactgga aggcgaacag gcgattgctg cttccgggtg cgcctatctc atcttacgat
     6781 cgtcctggat ttattcaaga cgcagcggat ttgtggccaa ggtgttggaa tgggcgcgta
     6841 cgcaggaggt tttgcggatt gtggaggacc aaatcagcag cccgacgcct gcgagattaa
     6901 ttgccgaagt aacagccagt ctggtttcaa gaattgcgga gcatccgcat gcctgggtga
     6961 atgaacgcaa agggatttat cactgcgcct gtaaaggcta ttgcagccgt tatgaatggg
     7021 caagcgaagt gataaggctt agctcacaaa aaaagtttaa tccgcagcta tttcctgcct
     7081 ctactgctga cttttctacc gccgcgcgaa ggccgctcag gtcttgcctg gatatcacca
     7141 aatttgagac aagctttgat atgaagttac cattctggcg aaatgctttg gagctcgcca
     7201 tggaggattg acccggcgag ggtttatggg aatgttcgtt tgtggatgag cgcttcactt
     7261 ttaggacgat cggtcgtttc gaatcagtga gttatcgatg aagatacaat ctgccgcagt
     7321 ttacagctat gaacgaatta ttgccagcct ggaagcctat cggctgttca atccaatgat
     7381 tcgtgcgaat ctgcgaatca acccggggat tgttgataat cagttttcaa cagacgctgt
     7441 cagggagtcc gatattgtgg tatttcagcg ggaattcccc gcccggcgct ccaattacct
     7501 ctctctgatt tccacggccg cgaacctggg taagccagtc gtaatggaca tggatgacga
     7561 cttgttggcg ctgcctacgc atcacccgga cagaataaaa ctcaattatg cgaaagcgca
     7621 aattccgatt ttgataggca tggttcagtc cgcggcgctc acggttacca cgccgtacct
     7681 ggctgatttg ctcagaaaat tcaacagcaa catctttatc cttcccaatt acctggatga
     7741 ttctctctgg aaatttaacg caccccaggt tgaacctgcg ggcgaaaaga ttcgcatttt
     7801 ctacatcggc acgatcaccc atgttccaga cctggaaatg ctcaaaccgg cttttcgcgc
     7861 tttggcgatg aaatatccgg ggcgcctgga gtttatgttc tacggggcta acctgcagct
     7921 ggaggaaaac attccggctg tcgtcaccaa ttgccagagt gaaacttttg tcttttcgga
     7981 ttatgtacgg attgcgcttg ctcagaaagc gaacatcgcc atagccccgt tggaagacat
     8041 cccatacaac cattgcaaga gttcgatcaa gttctttgag tatgccgcca tgggactgcc
     8101 aggggtttat tccagagtaa cgccttatac cagcgtgatt gaagaaggcg tgaacggctt
     8161 taccgctgcc agcatcagtg aatggattga ttccttgagt cagctgatcg aaaatccgga
     8221 actcagggaa aaaatggcgc tctcagcgca agcgactgtc cgccgggatt ggttattatc
     8281 cgatcacgct tacctgtggc ccgaaactta tgccaggata gcagaactca caaccccgaa
     8341 ccaggcaccg atcgctggct atttacctat cctggaaaat ataggcttcc acctggatga
     8401 cctgtttcaa aggcaggaaa aagaaaaatc catattcctc ggccgacaag attctttagg
     8461 tcgcgagatt caggaattga aagcccaggt tgatcagcta tcagttcaat ggttggacca
     8521 caccaagctg atccaagctc tggaaacaga aaatcatctt gtcaaagagg caagagacgc
     8581 ggcagatttg gagtgtgttc attacgccct tagcaaaagt tggcgcctga cgcgcccctt
     8641 gcgtaaattg aagaagttct tactgggaaa ataatgggat ttacatttac tgaccgacag
     8701 ttaattaatc gaagcgggtt gtttgacccc gtttactatt tgcgtaccta tcccgatgtg
     8761 cggattgcgg atgtggaccc gctcaggcac ttcatgagga tcggttggaa agaggggcgc
     8821 aacccttccg agaaatttaa tacccggttc tatctcaacg cgtacgcaga cgtgcgcgag
     8881 gaaggtataa atcccctgat tcattacatt tgttatggac gacgcgaggg ccgctcacct
     8941 ggataatcgc gggggatttc cgtgtagata gttgaacgcg ctgctaattg aatctgcgtc
     9001 ggagccagct tagaaagtgg ggtctgcttt tccccttggg gggagcgaac gctggcggtg
     9061 atgccttata gaccgtttcc tcatagatga ttggttttgc ggtgatttcg tagaacgaca
     9121 tttgcgtgag cccgtaccgg tcacgcagca ccccccgcat cttttcatct tgcagcaagc
     9181 tcaggaactt ttgcgcccct ccaagctgtg gaacgacacc gcagaatacc gttttgaata
     9241 gcagaagcat gcgttcgata atcaggtcat ccggatcggt ggtacttttg cgcgtaaaat
     9301 ccgctccaaa cgcttcaata aacagttcgt caggaatatc atcgaagaag ccgcaaaaaa
     9361 cctgtgagaa ttcaaaattc gcccggcgga tacctttttc cgaaggggcg ctcgcgttcg
     9421 cgcccgtggt gaaccaacgg taattggtta atctctcctg gataatgtga atcggataat
     9481 gcttgacaat cctgatccac aggtcataat cctgaacctg cacaaaagcc aggttgtaag
     9541 agccaacctc ctcaatgact ttgcggggaa acatagccga tggatgggaa acgccgcagc
     9601 caaaaaaata aaaatacctt aaccactccg cctgagtctt gttcttaaga tcccaccaga
     9661 gcacccgctc ggtatctctt tcagtgagga tattcccgcg ttcgtccaca aagttaacta
     9721 aggtgaaaca ggctccatat tccgggtggt tgcggaaaaa ttcaacttgc ttttgcagct
     9781 tatccggttc ccaggtatcg tcactgtcta tccgggcgat gagttctccg gatgagtggc
     9841 tgatgccgta gttaaatccg gcaaccatat gctcgtgtgt gtccgagtaa tacggtttga
     9901 ttctcgggtc tttgaaggat gtgatgattt cgcgtgatct gtcggttgag caatcgtcaa
     9961 agataatgaa ttcccagttc tgataggtct gggcaagcac gctttcaatg gtttctttga
    10021 taagaagctc gccattgaag ttggacatga cgatgctgac caatggattg ttattgctca
    10081 tcacttaatt tttctccaaa tacgcgaaac cctgttcagt ccgcgtgggc cggcaatttt
    10141 taatatcaat ttcttcgccc gcgtttttac ggcatttttg gttaattttt caggattggt
    10201 cacattgccc tgattaatca ggctgagata atattcgcgg ttttcaaaac ccactctgcc
    10261 agcgaaagcc gtgttgtagt ctttggtaat cttatgataa tttgtgatgt ccagttggct
    10321 gtaactggtg gtcattaccc aggctgagta ataaccggcc tgctgtgccg caaagggata
    10381 gacccgttcg atggagtgca taatcgtacc atcggttacg cgggtgggtt cgaccgggaa
    10441 atccccgtag ctatagttct gttcgaaaag caatgacagg gctttcgttc ggaaccagaa
    10501 aattgttccg agcggggcaa tcggcggcac ggcgtttgac atgggaactg tgatgcccag
    10561 ctcccgggcg tattgctcgg tcgcgtcgta gttaatcaac cattcattcc caggcaagct
    10621 tgaccatgga ccaaaattgg gggtgggagg tgtcagaagt ccaagcctgg ggttgtcctt
    10681 aaaagtcttc aggatattct gcacatattc tttcgagcca agcacgttct caaaacaatg
    10741 gtaagcgaag gattcgccga tgaggaaagg cttaaagtag ttcgtctttt tatcatgtgc
    10801 ggtacaaacc acatcataat ccatgatgta tggcttaaac cctaccaata acgcgctgac
    10861 gtcgcgcccg cggttttcga tcattaccac tttgacattt cgatctggta aggctgcaaa
    10921 aaccctttca atggcttcct tcttctccgc atcatccgtc gaaataaaga tgtccatgtt
    10981 atcgggcgta tttttggcat aacgcaaaca gtaatcaatt tgatccggat aaaaaatatg
    11041 caaaaaaagg gctgctttca ggtctggttt atcatctgga agccggacct ggtcgggaag
    11101 gatatagtta agctggaggc gttccttgat atcggacatg tgggctgttc tgagcagcac
    11161 atcccaaatc aggttcaggt cgtaatccgt tttttgatcc aggaattggt acaaatcacg
    11221 ggctgcctgt ccggggctta cctcgatgta ctcgttataa atattgtaga agaccttacg
    11281 cttaaaaaca gggcatccac gcttctccac caattccctc gggtaaagca tcagaggata
    11341 ttcgtggtac tctttcaggt cgtccgtatt cacatagaca tcggatttaa accccatacg
    11401 ggtgaaatct tcagtaaaga tagcctcatg gtacccgatc gattcttcat aggtacgaac
    11461 catcggtatg gtttcccaat aatgttgaaa ggctttggat tgaataaaat ccgagcgaaa
    11521 gaccatgaag ctggattgaa tatgcatggg aacgaaccca tacttgcatg tcttccatgg
    11581 gtcaaaggga agcccataat ggcgggaaag accccaaaaa tgcacattcc gctccgccat
    11641 cccgtcaaag gcttctttga atggatgcac cgggcccatg agcgtgtagt taagcagaac
    11701 cacttcgtca tattcagcca gcttcttcca gcccaccgaa agcatggctt ctttgtacgc
    11761 ccaaacatcc aggcctttat tctcacgttc aaggataacc ggatgaaact cagcgagttt
    11821 tgccttgctc tcggcgctca atggtccatt gctgacaaaa atgagttttt ccaggttttt
    11881 gaccagatcc cgtaacaaaa aagggatgta atcgtctacg ataccgtctt tatcgtacag
    11941 aaagtaaatg gcaattcggc ggattggatt ggttcgatcc agtttcatgg ctgttagcgt
    12001 ccggcggcag caattattct ttgaattttc gtgttatccc cgtagacgac aggagaatcg
    12061 tagggtctgt caggaaaaac gccaaattgc ggtttgatgt tcaggtgctt tgttgcgatg
    12121 aactcttcca ccttctcgcg cagggtggta gcttttccgc tgcaggtatt aatgatgcca
    12181 gtcacctccg actgcgtgat tgtccgcgcg atttggcagg caagctcgtc aacctcgatg
    12241 aagtcaaacg cgtttttccc ggtagtcacc gggaaggtgg catccccgcg ttcttcagcc
    12301 tcaagaatct tggtaaagat ggaatggttg cgtttatcat cgccaaaaat ataataggcg
    12361 cgcagccatt gaaaaaccgc gttccttgat tttgacaaga ggaaggtcag ctgacgcaag
    12421 gcgtttttcg agatgccgta atagctgatt gggttggtcg gggtattttc gtcaattttc
    12481 ccttcccaat atccaacctc gtgcatggta ccctggaccg cgatgtgttt taagcctgcc
    12541 tggagcatgc cggaaataaa gcggaaatgc ccgggtaaat cttcgatgtg gctttcggca
    12601 aagtggttga aaccgtttcg ccaggcaaga tgcaggcaaa catccacctg ggaggccaat
    12661 tcgaaaacct tttcgggttc cgtaaataaa tttacttgca gcccctgtgc gcgcggatca
    12721 attccatcaa gcgctaaatc agccgcgata acattttccc cctggtccag caattgtctg
    12781 gttacttttt ggccaatata cccattcgcg cctgtaacaa ggatttgctt tgtcacgata
    12841 attacacctt cgtcctttta taccatctaa attctatcat agcagcagtt tgaccaggcg
    12901 gccacaagag attgcggttc ttcgcaagca gctctgccta ccgcgtttcc ggccggcttg
    12961 tgggtggggc gccggggcat gcttttcttt tcgattataa taaaaaattg ctatggtttt
    13021 ttcctcccag atctttctgt ttgttttttt acccctatgc ttgcttgggg catatctatc
    13081 ccctcccaaa tttcggaagt attttttaat ccttgcttcg ctcggcttct tttcctggac
    13141 cgggaaatta ccgctcgcca tttttattgc ctttatcttg ctgaactatg gctttggtct
    13201 gctgctggga aagaccaggg aagcgggctc gtttggcaaa aaaatctcca tttggattgt
    13261 catactatcc aacgtgattt ttctccttgc ctttcgatac cagcccttta tcacaagttt
    13321 tctgacggac gctgtcggcc ttgaacttcc aaaattcgac ctgccagtcc tgttaggggt
    13381 ttccttcttt gtgttcagcg caatctcata tcagtacgat atttattacc ataagatcgc
    13441 gccccaaaag aactttgttg atttcttgct ttatatgagc tttttcccga agctcttaca
    13501 gggtccaatc acccgggcgg gggatttttt agcgcaaata acagaaagca aactgaatgt
    13561 cgatcaactt tccaggggga tataccgctt caccgtaggg ctggcgaaaa aggtcatcat
    13621 cgccgaccag ctgggcgtca tggtcgatca aattttcgcc aaccccgcca acgccaatgg
    13681 ggtttcggtt gcctggttgg gcgcgttcag ctacgcgatg cagattttct ttgatttttc
    13741 gggttacacg gatatggcta ttggtctggg gcaaatgctc gggtttaacc tgccggagaa
    13801 cttcaacttt ccatattcag ccctcagcat ttctgatttt tggcaccgct ggcacattac
    13861 cctgaatagt tggttccgcg attatgtgtt ttatccgctt gaattcaaac gcagaaagca
    13921 aaaggtcttc cgcacagaat ccaacacgct ggtcgttttc tttctgaccg gattctggca
    13981 tggggccggc tggcaattca tggtctgggg gctgtggcat gggctgtttg ccagcctgga
    14041 ggctttctta agaaacaaga aagtgaaagt caaagctcct gcgctcatcc gctatctcgc
    14101 gaccatgctg atcgtgctgg ttggctgggt tttgtttcgc tccccggacc tcaaatacgg
    14161 gctccagtac ctgggcgtca tgtttgggct ggtgaagccg attgatacag gcttgatgct
    14221 ctcctggtat ctcagcggga aaatcgcctt tatccttttg ctggcgatat tggcttgtat
    14281 cccttggaaa catttgctcc ccaaattctc cgaaaaaatc tcgggatcag cgctgaatga
    14341 tccggtcaga ctgattgttt ttacagtatt gcttgggttt tcgctcatgc tggtaatatc
    14401 ttccacatac agttccttca tctattttaa attctgatgc ggcgcgaatg aaaacagtac
    14461 catgcaggtt ccttctcatc gtcatttttc ttgctgcttt acttcttcca ttagtgctgg
    14521 cagataaagt tgggggagcg ctttccccta cggaaaaccg caacctggcc agatttccgc
    14581 agatattaac gcaggagaaa aagattgcgc cagggataaa aattgggttt gaagcctggc
    14641 tgaaggacaa cctgttcgga cgaatggagg cccaaaaggc gcgagcgtac atcgacttcc
    14701 gagtgctccg cacgtcccct tcaaaattgg tgcaaattgg ggaaaatggc tggtattttt
    14761 acaacaacga ccagaatctt gagattgggc ggggagccaa gctgctgtct cctgaagaac
    14821 tggaagcaat ccgaatgaat cagctcgcca tccaaaaagc tttgaaggcg caaggcatcg
    14881 aatacatcct ggtatttatt ccaagcaagg ccagtgtcta ccctgaatac atgcaggggg
    14941 gaaagtatcg ggtgggcttg acattaattg accaggttac cgcttacctg cagcagaata
    15001 caaccatacc ggtcatcaat ctgaagcctg atctgctggc ggccaaagcg gagcaggatg
    15061 tttatttccg atccgatacg cattggaacc aggccggagc gtatatcggg tatcgggggg
    15121 tcatcaaccg tctgaatgaa tggaaaatga ttcaaagtca accggtcagc atatccacag
    15181 aacccgcgct gcataccggc gagttttcat atatcatggg gtttccaggc ctgatttccc
    15241 ccgaaccgat aagagcgacg atcattcaaa accccaaagc ttctttggtg agcagcagtg
    15301 ataaaacgga gctggtaagc aggctcctgg cagaaacggg cataaagggg gagtatttct
    15361 catacaccaa ccctgatgcc gagagaaagg ctctgattct gggagactcc tttttcttca
    15421 cctggaagat tcccgatctc tttgctgaaa acttcgccga aatgaatttc attcgaacgg
    15481 atgtgcttac cagcgatatt atccaggctg tgaatccgga tatcgtgatt atggaaagag
    15541 ctgaacgata tatctacaac ctggctaaca gcagcaatgt gaacctgctc atgcccaggc
    15601 tgagcaatcc ttcggctgaa attatctccc acgacacccc cacgacaatt gaacgggata
    15661 aatcttacga cgtcaacatc gtggttaaga acaccgggga tgaagcctgg agtgaagaaa
    15721 agcatgttcg cctgggtatt ttccaggacg ggaaagacta cggttaccgt atctacctgc
    15781 ccgaaggcgt cacagtcgag cccgggcagg agtacacctt tgtgctctcc aatttccggg
    15841 caccggaagg aaacacaact tatctggaat atcagatgct gcaggaagct taccagtatt
    15901 tcggggaaaa agagcgcgtg gatcttgtgg ttaaataaac tcgcaaaaat caaaaactat
    15961 tggcaggatg ctatgacagt accttctttc ccgaacaata atattggcta cctttgcacg
    16021 gattggcagg tcgaacgcgg gcttggacaa aaaacagcgc tgatgtttct cgacaggtac
    16081 gaaacattac aggaactcag cttcgcggaa ctgaccattc tgaccaataa agcggcaaat
    16141 ttactttcca ccctgggttt tcgaaaaggt gaccgggtca tgcttttgct ttccaaaatc
    16201 ccggaaatgt ttgcattttt tctgggcggg atgaagctgg ggctgaactg ctgcatcctc
    16261 tttaccagca ttggcgagga ttccatcatt gagcgcatca gggataccca tacccgggca
    16321 attatctcca catcaagaat gaacttcaga ctggaaaagg tggttcaaaa ccttgacgag
    16381 gattttcgca tcctgttagt cgatcaaagc cgcgccgaag ggcgatttgt cggtatccaa
    16441 cagggttttg agacggcttc ggcggacttt tccactccag acacgaatcc cgagcaatat
    16501 tcccacttcc acttcacctc cggttcgacc ggcaagccta aagcggttca gcactgccac
    16561 ggggcaatca tcgaccacct gcgcagcttt gaagccgtta tgcaagctga cccggacgac
    16621 ttgtattggt gcactgccga cccgggctgg gtgaccggca caacctacgg gattaccgcg
    16681 ccctgggctt ccggcctgac ccaaattcaa ctggacgcga actacgaccc ggaactttgg
    16741 atgcggatca tcgagcacca aagggtgaat gttttttata ccgccccgac cgtgtttcgc
    16801 atgatggctc agcaaactga cgccttctat cagcagtata acctgcgctc gttgaagcgg
    16861 gtctatacgg ttggggagcc tctagatccc aacctggtcc tctgggggcg taaagtcttc
    16921 caatgcgaaa tctatgacac ctggtttcag actgagacgg gcagtatcat ggtcgctaac
    16981 cgcccgggtt tggaggtcag gccgggctcg atgggcaaac cgcttccata tcttgaggcc
    17041 gcgattttgg agagcaagga tcaccctttg ccggaaaata cccagggact gctgtgcatt
    17101 cgaaagccgt ggaaatcaat gttccgggag tatgtgggca gccccctggt ttaccaaaac
    17161 aaattcctgg gcgatttcta caactcaggc gatatcgctt atcgcgatga ggacggatat
    17221 ttctggtata ttggcagaag tgatgacgtt atcaacaccg ccggtcacct ggtcagtccc
    17281 tttgaaatcg aatccgctgt aatggaagtg ccgcacgtat tggattgcgc cgtggtcggg
    17341 ctgcctgatg agctcttata tgaaaaggtg gtagcgtttg ttgttcacga ccagcaagct
    17401 ctgtcgcttc gaaaactgga catggagatc aaactgtttg tatcaaagaa ggttgcttcg
    17461 ttggctgcgc cgaaggaaat tgtgttcatc gagaaaattc ccaagacgaa aagtggaaaa
    17521 gtgatgcggc gggtgctaaa gaagcaatac ctgggtgaag acgtggggga cctctccaca
    17581 atggagcaat gaaatggaaa ctgctgagat gaaatcccgg atgaacaagg tttttcgtga
    17641 ggtatttgat aatccaggga ttgaaatatt cgatggaatg acatcaagcg acgttgtcgg
    17701 ttgggattct ttctctcaca tcaacctgat tacttcgctt gaaattgaat ttgatattga
    17761 gttttcacag gcagaagctt ttggtttcaa aaccgtcggt gagctgcaaa aggcaatcct
    17821 gagaaaaatg tccgcctgat ccacaaatcc tttttcagcg gggttgcttc gaggtttgaa
    17881 atttccttaa tcgcctgcca ctcaagcacg gcaggatgtt gactttcgcc tcgctgacgt
    17941 tttgtgcagt tgtccaacaa ccggcaaccg cggctgctcc tgctggcagt tccgacgccg
    18001 cccgagctta gccgtccctg ctatgcctgt cagaggcttc ttcctttggt tccccagcct
    18061 ggtcttttcc agctgctttc cttttgtgct ccgcagaaag cagcagcccg accgcgcctg
    18121 ccagcagcgc tgccgccgct atcagccaga gcggtctcgc gccagaaatt tgttcccata
    18181 cgcccgctgc ggcggttgcc gtgggcgtcg ggcttggcag cggcgtgcgc gtgggcgcgg
    18241 cttgtgtggt aggggtggga agatgcgtcg gggtgactgg cgggggtgtt ggggtcatcg
    18301 tggcgggcgg ggtgacgttc aggagcagtt tttccccggc ccacagcgtg tagttaacgg
    18361 tcaggttgtt ccagcgcagc aggtcttcca gcgcgatgcc gtagtatcgg gcaatccagc
    18421 taaagttctg cccctccccg acggtgtgat aatacttgcc gtccgcggcg ggggtcagca
    18481 tctccaacgg ggaaagcggc ctgggcgtgg gggtgggcgt gacgttgccc gggtcaatca
    18541 gtagaatttg gccgatttgg agcggcatgt caatcgtcag gctgttcagc gccagaatgc
    18601 gctcgatgct cacgccgtag acctggctga tcagggtcag gttttggtaa gctcctacgg
    18661 tgtgcgtaat tttcccgtcc ggcccgggcg ggttggcgct gaagtatcct accggcgtgg
    18721 gcgtctggta gcccggcgcg ttggggttgg ggatgaccag cagctgcccg acctggaggg
    18781 tggcgccttc cggcaggttg ttccacagca ggatttcctt gatggtgacc tggtacgcca
    18841 ccgcgatagc ccagaagctc tgaccggact ggacttcgtg cacgatgcgc ccgctggcat
    18901 ccggctcggc aagcgcgacc ggcacgatga taccgtaccc gccgcccccg tccactggac
    18961 cgcctgggga ggtgtaggtg ccgcattcct tgccggagac gtaggccgcc tgcaggatgt
    19021 aataagtgga gccgttcgag gcccgcgcga cccccgcgcc gacattacag tagtaggact
    19081 gggtggcggg gcgcatatgg tcggggtccg cccacatgag catgatttca tcgatgccgg
    19141 cgttgtagcc gatggcaaag ttttccgttg cccaaacggt gcccccgccg ccgtaaccgg
    19201 cagccgcgat gcgccctttc acgtcgccaa tatgccagga gagcagttga tccgccataa
    19261 tctgggcggt gctctgcgcc accgcatcga taatcgggtc ttcgataagc gcggtaagtc
    19321 cgttcgccat gcgcagggtg ttcatcgctg caatcaagtc ccccgcggag acgtcggcca
    19381 gcggcatggg ctcggccttc gccgcggctt tttgggccgg cagccaagcg aatgaaagca
    19441 gcagcagcgg caccaggagc aagcgaaaga ctttacggcg gggcatattt catatatacc
    19501 acaattttac gaggcagttt ttgagaggga aatccactcc cgggaaaata gtggggaaga
    19561 agcgctgccg acggcctgca gaactatgcc gggtaaggat gataaaatta gcctgggaag
    19621 taaactgcgc acacgaattg tgctttccaa ggagttcgcg gtgaaaaaac tggtcatcca
    19681 aatccctgca tataacgaag ctgaaaacat cgccggcaca ctggcggata ttccccgccg
    19741 ctacgaaggg attgacgaag tgttggtcgt cgtcattgac gacggctcca cggacgggac
    19801 cgccagcgcg gcgctggaaa acggagcgga cgcggtcatc cgccaccggc gcaaccgcgg
    19861 gctttcgcgc gccttcatct ccggcatcca attcgcgctg gcgctcggcg cggatatcat
    19921 tgtcaataca gacgctgaca atcaatacgt gggcggggag attcagcgcc taattcagcc
    19981 gattctggat gattccgctg acctggtgat tggcgaccgg aatccggcgc aaaacgcgca
    20041 tttttccccg ctaaaacgca ggatggaagc atttggcagt tggattgccc gcaaagtctc
    20101 cggaacgaac gtgccggata ccgtcagcgg cttcagggcg tactcgcgct atgccgccct
    20161 gccgctgcag gtgttcaacc cctattccta cacgctcgag acgctggtgc aggccggcaa
    20221 gagccagatg aaaatcgcgc acatcccgat taagaccaac ccgaacctgc gcgaatcgcg
    20281 gctgcataag ggtttgctgc acttcatctg gaagcaaagc ggcgtgattg tgcgctctta
    20341 tgtgctttac cagccgctgc gcactttcgt gtcgctgggc gcggtctttc tggcggctgg
    20401 cctggcgctg ctgctgcgct ttttgattta ttatttcatc accaatacgg ccgggcggct
    20461 gctgcaatcc gtcacgattg gcggcatgct taccattgtg ggcatcatcc tcatcatcat
    20521 cggcttcatc ggggattccg tgcgcgccag ccggcagatt tccgaggaga tcctgacctc
    20581 cctgagggat aagacccgcc tgggccagct ttccgatgtg cgcgaattca acgggcagcc
    20641 ggtttttacg cctgagcatc ccgttggaac aggtcagctc tgagctttct gggatgaata
    20701 agtgaggcag atcataatgc aaaacaagca gctgtattat gtttacgaaa attgggtggc
    20761 gaacgggcac acagccagga ttcacaaggc cgaatgcagt ttctgcaatt ccggcaaagg
    20821 aattcacggc acgcacaatg ttgagcacgg tcaatggcat gggccctttt cagttatcga
    20881 agatatggag aaaagcttct ctccaaacag ggtgattatt actaaatgca agatttgttt
    20941 cccttaggat ttggtggaat tcgagagttt gccaatgaat ccgacaacgg acagagaatc
    21001 tgaggtgtgc cacccgggag atcgcttcgc aagctcgcga tgacgtgcga ttcgtcatta
    21061 gtatgagccc ccgttgtcaa cctcacgcga aacaaagaac tacagaaagg gggcgcggca
    21121 atctgcctct gggaagtaat attctgttgt tttgttagag aatacctgaa atttgtttgg
    21181 aaactatttc cacccttcgg gcaaaccttt caggaaataa aggcgaatgc cgcgaccctt
    21241 tgccaggcgg cgctcccacg gattttaagg gtatggtggc aaagggtctc gcagtgaccg
    21301 tcaggcaggg cgcgctgcag caaaccattc gtctttgcag cattgggaga ctgccgattc
    21361 gtcgttgcga gcccccgttg tcaacctcac gcgaaacaaa gaactacaga aagggggcgc
    21421 ggcaatctgc ctctgggaag taatattctg ttgttttgtt agagaatacc tgaaatttgt
    21481 ttggaaacta tttccaccct tcgggcaaac ctttcaagat attaaggcgg actgccgcaa
    21541 ccctttgcca ggcggcgctc ccacggattt tatgggtacg gtggcaaagg gtcgcgcagt
    21601 gaccgtgagg ttttcgtagg ttgggtgcag cgacgcagga gcgcaaccca acaacagcca
    21661 acacccgcac gtgcccgcta attgctcaac aagggtctag tgaccgtgag gttttcgtag
    21721 gttgggtgca gcgacgcagg agcgcaaccc aacaacagcc aacacccgca cgtgcccgct
    21781 aattgctcaa caagggtcta gtgaccgtga ggttttcgta ggttgggtgc agcgacgcag
    21841 gagcgcaacc caacaacagc caacacccgc acatgcccgc taattgctca acaaatggcc
    21901 tgaccccgct ccaccaaagc tgccgcttca cgcgcgagca tcctttctac caaatttagc
    21961 attagaatcg cctgatttct gctttcggac gggggaattt ccgccagctt aaaatagccg
    22021 gaagttttaa aatttcccac ccaagcgcca aattccgtat gctatacttt gatagcggtg
    22081 gacgtacacg ggtggcatgc ccaccacgcc aataattctt taaaggacca acccggcgtt
    22141 atgtcgtttg ttcatttgca cgtacactcc tcatactccg ttctggacgg ctttggtaaa
    22201 ccagcagacc tggttgccag ggcgcgggaa ttgggcatgc ctgccgtggc gctgaccgac
    22261 cacggcacga tgttcggcac gttggatttc ttcaaggaag ccaacgcggc cggcatcaag
    22321 ccgattatcg gcctggaaac ctaccttgcc ccgcgctccc gcttcgataa ggacgtcagc
    22381 aaagataaaa aaccctttca cctggtgctg ctggccgaaa acatgcaggg ctacaagaac
    22441 ctgctccaaa tcgccaccgc cagccagttg gaaggctttt actaccaccc ccgcatcgat
    22501 aaagaatacc tcgccgcgca cgccgacggc ctgattgcca gctccgcctg cctctccggc
    22561 gagctcccgc gcgccctttt ggagggcgac cccgaaaagg cggaacgcgc gctgggctgg
    22621 tacctggacc tgttcggtcg ggagcgcttc ttcctggaaa ttcaggacca caacctgccc
    22681 gaactgaacc aggttaaccg cggcttgctc gggctggcgg ataaattcca ggcgcgcatg
    22741 attgccacga acgacgtgca ttacatccgg cgcgaagacg ccgaaatgca ggatgtcctc
    22801 ctggcaattc aaaccggcaa gctgctcacc gaccagaacc gcatgaccat gacggacgac
    22861 tcgtactacc tgcgcagtcc gcaggaaatg cgctacctct tttccgaaat cccggaagcg
    22921 ctcagcaaca cgctcgaaat tgctgcccgc tgcgaggtgg acctgacccg taagggttac
    22981 cacctgcccc ggtttgagct gccaaacggc gaagaacccg gcgcttacct gcgcgaactg
    23041 tgcgtggaag ggctgcgcaa acgcatcccc gcggaggcgg acttgccgga gacgcgcgaa
    23101 cgcctggatt acgaactcag cgtaatccaa aagatgggct ttgacgaata cttcctcatt
    23161 gtgtgggatt tgtgccggca cgcgcgcgaa aagaacatct ggtacaacgc gcgcggctct
    23221 tcggctggtt cgctggtagc cttcgcgctg gagattacct cggttgaacc gatccggcat
    23281 aaattaatgt ttgagcgctt tttgaaccct gaccgcgtga ccatgcccga tatcgacctg
    23341 gactttcagg acgaccggcg cgcggatatg atggaatact gcaatgaaaa atacggcgcg
    23401 gataaagtcg cgcagattat caccttcggt accatgggcg cgcgcggggc catccgcgac
    23461 gtcgggcgag tgatgaacat cccgctgccg gaagtggacc gcgtggcaaa aatcatcccc
    23521 ggaccgcagc agggcaagac gcccagcatc gccgaaacac tggaaaagtc ctctgaactg
    23581 cgcgcggtct acgaatccag cgaccagatg aaaaagctga ttgacaccgc cagccgcatg
    23641 gaaggcggca tccgcagcgt gggcacccac gctgccgggg tgattatcag cgacgagcct
    23701 atcacggaat acctgccgct gcaccgcccg acggggcaaa acgaagacct gccgattaag
    23761 tcggtcgccc aatacgatat ggacgggatt aacgacctgg gtctgctgaa agtggacttt
    23821 ttggggctgg tcacgctcac catcatggcc aaagcctgcg agctgattga acgccgccac
    23881 ggcaggaagc tgacgctcga aaacatcccc acgggcgacc ctgaagtcta ccgctacatc
    23941 agcgaggggc atacggtcgg gatgttccag ctggaaagca gcgggatgac cagcaggatt
    24001 atgcagatgc agccgaccga actggcgcac cttatcgcga tgatcgcgct cttccggccg
    24061 ggtccgatgg acagcattcc ggaatacatc gaccggatgc acgggcgcaa agaggttaag
    24121 tacgcgcacg aaaagctgaa gcccatcctc gaggaaacct atgggcacgc ggtctatcag
    24181 gaacaggtca tgcaggcggc cattgccctg gctggctatt cgcccgcgga ttcagataag
    24241 ctgcgctcgg ccatctccaa aaagaaagaa aaagagattg ccgcccaccg cgtgcagttt
    24301 atcgaaggcg cggtaaagaa cgggctgacc cggaaagagg ccgagtccat cttcgcggat
    24361 tgggaaggct tcgcgcatta cggctttaac aagagccacg cggcggatta cggcgtcatc
    24421 gcggtgcaaa ccgcctacct caagtattac tacccggtgg agtatatgac cgcgctgctt
    24481 tccgcgtgga agaacgacat cgaaaagtgc gcgctgtacg tggccgaatc caaggcgatg
    24541 gggctggaag tgcttcctcc ggacgtgaat accagcggat acgactttgt gattgaggac
    24601 cgcccggatc agagcgccgc catccgcttc ggtttgggcg cagtaaaaaa cgtgggtcag
    24661 gctccggtgg atttaatcat cgcggcgcgc ggcgggagga acttcaccaa tatcaccgac
    24721 ttcgccagac gggtggacct gcgccaggtc ggcaagcgcc cgttggagtg cctgataaaa
    24781 gtgggcgcgc tggatagttt tgggccgcgc cattccctgc tgagctcgtt ggagcagctg
    24841 gtttccgtca gcgcggcgca tttccgcgcg gtagaattgg gtcagctcac gctcttcagc
    24901 ggcgcgcagg agcccggtgc ggaggtcaga ctcttcgcgg gaagcgcggc agaccataac
    24961 gaacagctgg agtgggagaa ggagctgctg gggctgtacg tttctgacca tcccctgaat
    25021 acctacatga gcctgattaa cggtcagatt acccatctgg cgaaccagct ggaagagctg
    25081 aacaacaaag aagctgtggt ggtcgggggc ctggtaaaga aggcgcgccc gattttgacc
    25141 aaaaagcagc aggaaatggg ctttgtcacc attgaagata ataccggcga aatcgaactc
    25201 gtgattttcc cgagcgtatg ggagaccagc gccagccaga tagaaggcgg cgcgctgttg
    25261 ttggtaaaag gcaaggtcga tcaccgcgaa agcagcgtca gcatcctggc tgaccaggtt
    25321 acacgcgtgg aaaccagcag cttgaaagat cccctgacgg ataaccgcaa ccagggacct
    25381 tattatgagc aggtgctcgc gaaatacctg cccgatatca gcgcgctcag accttacgcc
    25441 tttgacgccg gcgccgaccg caattctgag ccggacgcgg ctccccgcag caccgaggca
    25501 gcgcaacccg cggacccctt cgaggaaccg gaatgggagg agagcgcttt ttccagcttc
    25561 ccgcctcctg ccgcgcctga acccgaagcg gaactggacc tgccagctgc cgaagcggac
    25621 cctcccgccc atgctgaagc tgaaggcgct ccggctcagc ctttcagcgc cgccctgccc
    25681 tctgatgcgc cggcgcaaac ggaagtcgtc cgctctccgc gtttgttggt gaccatccaa
    25741 tcctgcggtg aaaaagagcg cgatttgcgc cagctcaagc aaatccatgg gtttctcacc
    25801 tcgcaccccg gcgaaaaccg cttcttcttt tacattgcgg aagcaggcgc cacatacgag
    25861 attgaattcc cgaacgagac gaccgatatc aacgagcgca tcctgacgga actcaaccgc
    25921 ttcctgggcg gaacgaatgt acagttagcg gtggacggaa actgaacaca cggagaaacc
    25981 tatgcgggat gtggacctcg attttaatcc tactgaagtt ttgattcttt ctgctattgg
    26041 caagccgggc gaacgcacct tcttcattcg cggggggaat aaaagcctga ccatcacgct
    26101 gcgcatggaa aaaattcagg tacagatgct ggcggccggc gtcgtccact ttttggagga
    26161 actgaaaacc aatttcccgg agctggacga acccgccggg acctatgacg aaagccgcat
    26221 ggtgctgaaa ccgcccatcg acccgctttt ccaggtcgcc gagatgagta tggggtacga
    26281 ccgcggaaac gacttgttcg ttttcatctt cgagtcacct aaaccggaag aagccgcgga
    26341 agcgcccggc gtggtgcgtt tctggtgcaa acgcgccgaa gcggccggcc tggccgcctg
    26401 ggcgcgcgag ctgatctcgc gcggcaggtc aacgctggct gaacccggca gtgccgggga
    26461 acctgacggg gagatttcgc cgcgcaataa cgggcacaag cactgattcc cggaatggac
    26521 gaaccaacgc aactgcgcgt gctccaacac gggcaggtca gtcttctggg tcagttcacg
    26581 cgcagctcca acttcacctt tctggtcgaa tgtacgctgg aagcacacag cctgcgggca
    26641 gtctacaaac ccgcacgcgg gcagcagccc ttgtgggact ttgccgcgga aacgctgccc
    26701 aagcgggaaa ccgctgccta tctgctctgc cgcgcgatag gctgggactt cgtcccacca
    26761 accgtttatc gcacggaaga cttgccggct ggcgagggat ccttgcagtg gtttgtggag
    26821 cacgacccgg agcagcatta cttcactctg accgaagctg aacgcgcctc cctgcagccc
    26881 ctggcgctgt ttgacctgct ggcgaataat gccgaccgca aaggcgggca tatcctgcgg
    26941 gatgaggcgg gaaaattgtg ggcgattgac cacgcgctgt gcttccacgc ggaaacgaaa
    27001 ctgcgcacgg tcatctggga ctttgccggt caaccgcttg gggaggcgct gctcaacagc
    27061 ctggacgcgc taaacctcag gctctcgccg gggcagcctt ttctgcggga aatcagccgc
    27121 tacttgagcg ccgcagaaag cgacagcttg cgcgaacgct gccaaaaact gctggcggat
    27181 gggcattttc cttttccagc ggaagaccgc cgtgctttcc cctggccccc ggtctaagcc
    27241 gcgccgctat actataattg agagcaaaac aaatacatga ggtgaacctt gactgaaaca
    27301 gtccgcttgg tgatggttgg ttttggcaat gtgggcaagg cgtttgcccg gctgctgcta
    27361 aacaaggatg ccgaactgtt ggagcgctac cagctgaaac tcctggtgac cggtatttgc
    27421 acccgctccc acggcagcgc gattgacccg cgcgggctgg acctgcaggc agtgctggag
    27481 gccctaaaaa cgggcggctc gctcagcggg ctggataccg cgggcttcag cggcagcgcg
    27541 ctggatttca ttggagcctg cgaaggcgac gtgctgcttg agtccacgcc tgtgaacccg
    27601 aaatcgggtc agccggccat tgcgcatctg gaagccggtt tgcgcaaagg catgcacgcc
    27661 gttacgccca ataagggtcc ggtggtgttt gggtaccagc atctgaccgc gctggcggaa
    27721 agcaaaggga aaaagtttct gttcgaatcc accgtgatgg acggcgcgcc aattttttcg
    27781 ctcttccgca agaatatggc cctggcggag ctgaaaggct ttacaggcgt gctgaattcc
    27841 tgcacgaacc tgctcatcgg gcgcatggaa gacggcgaga gctttgcgca ggcgctggat
    27901 tacgcgcgct ccatcggcat caccgagacg gaccccagca acgacgtgga cggctgggat
    27961 gctgccatca aagtgtccgc gctggcgacc gtgctgatga agcacccgct gacccctgcg
    28021 gaagtgcagc cgactggcat ccgcgggatt acgcccgaga agattgccga agcacgcgcg
    28081 gcgggcaaac gctggaaact ggtctgttcc gcggggtatg acgagcaagg gcaatttttc
    28141 gcgcgggtct gccctgagat ggtaggccct gaatcgccct tctattccgt ctggggcacc
    28201 gactccatcg tggtcttcaa caccgatgtg ctgcccggtc tgggggtttt ggagaccaaa
    28261 accaccccgt acaccacggc gtatgggctg ctctcggatg tgctttccgc gctgagggac
    28321 tgagagcagc gctgtggcgg ttgtattcct ggggctgggc acgaatctgg gcagcagggc
    28381 tgagaacctt tccgccgccc gcgagcagct cgccgcgcag atgcgcctgc tgcgcgcttc
    28441 atccgtgtac gaaaccgaac cctggggctt ccgcgagcag cccgccttcc tgaaccaggt
    28501 ggtgcaggcg gaaacagact tgagtccgct caagctgctg cgtttcctca agcgcctgga
    28561 gaagcagttg ggcaggaccg agaccttccg ctacggcccg cgcctgatcg atatggatat
    28621 cctgttttat gaccgagcgg tcataaacac ccggcggctg caaattccgc acccgcgcct
    28681 gcccgaacgc gcttttgtgc tcgtcccgct ggcggaaatc gccccggact tcgtgcatcc
    28741 ggtccttgag cggactatga gggaactgct gagggactgc ccggatcaag aaagcgtgcg
    28801 taagtgctga gcgcgctggc tttggccgcc tttttgctgc ccgggctgtg ctggtgggtc
    28861 tggctggggg accgggacaa ggatccgctg gaagctttgg ctggcatcct gggcgtgagc
    28921 gtggcgctga cggcgttggg cgccctgttt ttctacactg tgcgcctgtc ggtttcggct
    28981 ggtctgctgg gtgcgctgct tgggttgacg ctcagcctga cgggatatgg tgtttaccgc
    29041 aggcgcaaag aaggcgtttc accctggggc tggctgcttg ctctggccgt atttggagcc
    29101 ctatgcgttt ggcgcctgtg gcaggcgagg gggctggcac tgccggcctg ggtcgattcc
    29161 ctgcaccaca gcctgattat ccgcaagatg atagaagccg gcggtctgag cagcacgctc
    29221 gaaccctatc tgcccggtcc gttctattat cattacgctg tccacagcgc ggcggcgctg
    29281 gtttccgcgc tcagcggtga tgcgccggcg caaaccctgc tctggctcgg gcagatcttt
    29341 acggcagggg tctcgctctc cgcttacagc ctggtgaaag ccagcaccag ggattggcgc
    29401 ccggctgcgc tggcagggct gctggttaca ttcgccacca aaatgcccgg ctattacctc
    29461 agctgggggc gttacaccct gctgaccggc atgctgatgc tgccgttggc aatggcggag
    29521 gccctgcgcg tcgcgcgcgg ggagcggctg cgccgctccg cgccgctgct ggttgtgctc
    29581 accgcgggca cgctgctgag ccattacctg gcggcttttc tgctggcgct gttcctgatt
    29641 gtgctggcgc ttgcgcagct tggccgaaac atccgcccaa aaaaccggga ctggcgtccg
    29701 ctgctggcgc tgacggcctc cgcgctggct gggttggcgc tggtttcgcg ctggtattgg
    29761 cgcgtgttcc gctattcacg cgggatgatg tcaaccgaaa ttcaactgcc tgcttccggc
    29821 gcgctcagcg cagaacactg ggactacttt tggaacctcg tcggaccact gacgggttac
    29881 gtggtgttgg ggttggcttg tgccgggctg gtatgggcat tcacccggcc caaaactcgc
    29941 ccgctggcgc tttggagctt gctcgtcggg ctgctttccc tgccgtttgg gctggttttg
    30001 ggaccgttcc gcagcgatca ctttgccctg gcgctctttc tgccggcagc ctgcctggca
    30061 gcttgtttgc tcgtttgggc tggcgattgg ctgcgcagca ggcatcccag gcaagtatgg
    30121 gggacggccg ctctgctctt ggtctgcgcg gcgctgctgg gaggaggcgc ctggctgaac
    30181 cgcgaaccgg tcaatgccag caccgtcctg gcagatgaaa gcgacctgaa ggcgctggag
    30241 tgggttgaag accatttgcc ctcagacgcg cgcttcttta tcaataccac cggttggggc
    30301 tacgggctgt accgcggcat ggacggcggc gcctgggtat tgccgtacac gggcaggtgg
    30361 tcgctcgcgc cgactatctt ttacactttc agcggcgaag agcagtctta tggcgcctgg
    30421 attgactgga gccagcgcgc caacgtggtg acgggctgca ccgaggagtt caaggctttg
    30481 atgaccgagg ccggcttaag ctatctgtac ctgcgggagg gcgtggggag cttgcaggcg
    30541 aatgctttgc ggggctgccc tgaggcgaag cagctttatt ccaccggcgg ggtcagtatt
    30601 tggctgtggg atgacgcatc gatcgatgct ggacaaggag aatagcgcgg ttttctatat
    30661 aggaatggtc attctgcccc agcttttggc gcttggttgt gaacgaaatt ccatgggctg
    30721 ggtgatgatg ctttcaggat cattccaacg gaatacgagc ctgtattcct gcgaaaaccc
    30781 ctcaatgata tgattgccgc attggaatct ttaccaaaac cccagcgcaa tttttcgagg
    30841 tgagatgtta ttaactttat cgatttggca gtggtttaag ctggcagctt cgtttttctt
    30901 gttctttatg cccggcttcc tgttgatcgg ggcattttgg ggaaaacgaa ccataagctt
    30961 tgggcaaaaa acctccctgg cgtttgggct cagcctgggt gtatggtgcg tgctcctggt
    31021 atggctggat ttcctaaatc tatctttaaa cacaggcctc gtgcggatgt tttttgggct
    31081 gtgcctggct ttcgtcatcc tgcagatagc agcgacgaga acgcttcctt attttccccg
    31141 ggcagggatc caggggttgg aaatgttcat tactgtgctc tccgccttgc tcattttatt
    31201 ctttgcctat ttgcttagag atctcgccag cggtatgggc agtgacagta tgcaccatac
    31261 gcttatcccg atgcttatgc tcaaatacgg gcagatccca tcgaattatg atctgtttgc
    31321 gcctgtaagt acgtttacgt atcacttcgg ttaccatgcg tttgtctcag ccatctcatg
    31381 gttaagcggc gtttcccctc agattgttgt gattctttcc ggggcctttt ttgtgggcct
    31441 atgtgctttt attgccgccg tgatgataga aactgtaaca aaaaacaagc tcatggcgct
    31501 ttgcgcggtg cccttgatag ggctgattta tatatttcct tcgtactcac ttatgtgggg
    31561 aagatacacg cagtttctgg cgacgatgct gctgatggtt ctgattactg tcattctggt
    31621 atcagaggag acaagcgggt tttcccgcaa agatattcct ttatttgtct tgttgacagc
    31681 cggcctgttt ttctcgcatt atcgcatctt ccttgccgct tgtcttttcc tgggctgtta
    31741 ttttctggtg cggcttgtaa agatcaagga aaagaaagag tatttttgga cctgggtatt
    31801 cattggcttg ggcaccgcgg ttgtcagctt gccctggata atccgacttc tggacgtcag
    31861 gtttgggagt ctggggaacc gcgtcctgat tagtgacccg acatatttct ccctggcaag
    31921 attaggcccc ttgttcgccg gggaatttca gaatattttc ctgattattc ttgcaggcgt
    31981 atcaggcctt tttgggatcg cgaggaaaaa cacggtcgca ttaagcgcga tgatctgggt
    32041 cgcgttgatt tgggtcctct cactgcccgg gaaaatcgga tcgctcctgg atccggtcac
    32101 gatgatgata tcttcttatc ttcctgttgt gctgctgctc cttatcggcg tcgatcacgc
    32161 ctaccggatg ataaagaatc gacgcatcgg gcttctggtg aacttgttgc ttgtttgcgt
    32221 agtcgtttat gctgccggga ttggcattgt gcgctgggtg aatattgatt ttgagcctga
    32281 ggaatttgtc acctttgaag acgtgcaggc tggcaaatgg attgagcaaa atacgccgtc
    32341 agacgcgctc tttgtcacca atacctacca gttctcattt caggaagaat tggtaaccgc
    32401 catggatgcc ggctactggc ttccggtgat tgctcataga ccaacgctaa tacccccgat
    32461 ggtttatctc atcgaacgca tgaacgcgga agacgtccaa tatcgtgggg ccattgttca
    32521 ctcctttggc gggcacctcg gcagtcagga ggccgtcaaa tacctgctct ctcaaggcgt
    32581 tgattatctg tatctcggcg ccaaaggcgg aatcatcaac tcctctgaaa ttgacgcttc
    32641 catggattac gcccttgtct ataacacaca gtccgtcagg gtttataagc ttctggagca
    32701 ttaaaaaaat tgccctctgc cggggtgaat gacgcaaaag gcgctgctct ccccgataac
    32761 gaagcgtgag ttccgcgcgc agcgtttgtc cagggcgtgt ccggatttag gctgcgttcg
    32821 aaaatgcctg gtctgaagta aaattagcgt ggcgtaaatc gccaggaaat cttgaaggct
    32881 ttgttgaacc aatgaaaatc ctcatcgcga ccacgaattt cccgcgctgg caaggcgact
    32941 tccgcgtgcc ttttatctac gaggccgcca aagccctcca agcgaagggc aatactgtgc
    33001 gcgtggtcgc gatgcacaac ccgggctcgc gggagcatga gctgatggaa gggctcgaag
    33061 tcttccgcgt gcgctacctg ccggaggaaa aggaaaagct gcagcaggat gccgccggta
    33121 ttcctgccgc ctgggagcgc gggctgaagg gacgcctcgc gctcatacca tttctcgtgg
    33181 cttataccaa agcggtgggg cggcatgccg cggattgcga catcatccat gccaattggt
    33241 cgctttccgg gctggcagcg ctgctttcgc gcgggcggca taagctccct tacgtggtta
    33301 ccgtgcaggg cagcgatgtg ttcaagactc tgaacaaacc gggcgtccgc cagatggtgg
    33361 ggctggcgct gcgcaaagcc ggcaaggtca ttgcgctcag ccgcgcgctc aagagcgccg
    33421 cgctggaatt tggcctgcag gaggaacacg tggtcgtgat tccgaacggc atcaacatca
    33481 gccagttccc gtttgcaacg ttggatgaaa agaaaaacca ggtcctgttt gttggttcgc
    33541 tgattgcgcg caaaggaccc gcaacactgc tgcaggcaat gcgcttggtg caccaggacc
    33601 ttcccgatac aaagctggtg ctggtgggcg aaggggatga acgcgcccat ctcgagaagt
    33661 tcatcgcaga gcacgacatg gcggattacg tctgcctggc gggcacccaa acccaaagcg
    33721 cggtcgcgga actgatgcgc gcctcccggt tgttcgtgct gccttccatt gaggaggggc
    33781 agggcgtcgt tttgatggag gcgctggcca gcggcacgcc ctgcgtgggc agtaatgtgg
    33841 gtggcatccc ggacgtggtg acggaagatg tgggccggct ggtggaaccc ggggacgcac
    33901 acgcgctggc agaagccatc cgcgcgtacc tgaccgacgc agaactgtgg caaaaagcca
    33961 gcgccgccgg ccgacagcgc gttctggagc attatgactg gaatcacctg gcgggcgaaa
    34021 tcagcgcggt ttacgctgaa gtcctggctg aaagaagcac atgagagaat accaaaaaga
    34081 ctttatcacc ctgtatgacg attttcagga tggcgaaaac cgcaagcgta aagcggaaaa
    34141 aatcaatacc atccttgaaa aactcaagct gcacaacccg cagggcactt gcctggatac
    34201 cggctgctcc aacggaatta tcacccgaag catggcggag ctattcgcgg ccgtggtggg
    34261 ggtggatatc gacaggaccg ccatgaaact ggtcgacccg cgcgaaaacc cggctgtttc
    34321 ctacgtctac ggggacgcga tggggctgcc tttcgcggat gaagctttcg aagcgctggt
    34381 atgctcccaa acctacgagc acgtgccttc ctcaccgcat ttgttcgctg aaatcctgcg
    34441 tgtgatgaag ccgggcggcg tggtcttttt cagcgggccg aacaaggtct tcccgattga
    34501 gccgcattac tacctgcctt tcctgcattg gctgccggaa aaatgggcag acgggtattt
    34561 acgcctgacc aaacgcggca cgcattatta cgagcgctcc cgcacctatt gggacttgcg
    34621 ccgcactttc ctgaaggatt acgaggtaca cgacgcgatg cgttatgtgc tgcagtacta
    34681 cgccctcacc aacccgaaaa agggcatgcg ccggctctac agcctggctg ccaagctgcc
    34741 gccgttcttg ttaaaactgc tctcaccgtt catggtgaat atcaattggg tcctggtcaa
    34801 acgcgaagca gggaaaaatg gcgcaaaaaa catccaattc gcgcgttagg cggcgccggc
    34861 aggagatcga gcgcgagctg acccgctcgg gcccggacgc gtggaaatac ttctccacgg
    34921 ttatttattc ccattggagg attgttattg aaggctgcct gccaaagtat ctgcagggac
    34981 ttgccctgga cgtgggcagc ggcgcgtgcc cctaccgcgc gcttgccgaa agccatgcca
    35041 gccggctgct gctgatggac cacaacgcgc agcccatccg gattgacttg cgcggcgata
    35101 tccaaaacat tccgctgcgc gctgaggcgg tggacagcgt tttgtgtttc caggtgctgg
    35161 agcacgtcgc caatccattt gagggcattg ccgaaatcgg gcgggtgctc aggccgggcg
    35221 gcattttgct gctttccgtc ccgcatctga gccgcctgca cgagctgccg cacgattact
    35281 accgctacac cgaatacggc ttgcgggaac tggcaaaagc cggcggtttt gcggttctgg
    35341 agcttgaacc ggtcggcggg ctggtttcct ttctggggca ccagctttcg atggcatatc
    35401 tcaccctctt ttggccgctg cgttttctgc gcccgctcac actattcatc aataaaaact
    35461 ttttcacccg ctttctgacc tttgtcgatc ggtatctggg gttcaagacg ctctttccgc
    35521 aaggctatgc gctggtcgcc cgcaaactat gaagaattgg ccgcagatac gcagaattct
    35581 ccggattgcc ggtacgctta tcggtctggt catctttgct tatcagctct ataccagcat
    35641 cgggtccctc agcacccaaa aacagcagct tcagctcatc tggggctgga ttttcctggc
    35701 gttggcgctc tacctgttgg tgtacttgct gcagatggtg aatttgtggc tggtgtataa
    35761 aaccaatttt ccggcaatca agttgaagcc cattgttcag ggctatgcca tgtcgttcct
    35821 gccaaaatac accccgggct acatctgggg gtatctttcc cgcgcggact ggctggaaag
    35881 ccgcgcgggc attccgggcg cgtattcctg gctggcgacg gtactggaag tggtcgtcac
    35941 gctgatgtcg ggcgcggtgg tgctgctgct taactgggcc gcccggggtg gggcttacct
    36001 ggcgctggct gtgctggcgg gtttgctccc ctggctggtc tggctgttga tgcgcctcgt
    36061 ggcccgcttc gcgcgcaaat tccttaagga agagcggcag cgtgttttgg aaggttttgc
    36121 tattccgttc gcgcgctggg cgcttatcag cgtgaattcc gtcgttcaat ggctgctgtt
    36181 gggcgtcggt ctgcgtttga tcagcgcggg cttttcgtac caaactccgc tgccgttttg
    36241 gtccgcgctt tggaagcata tctacgcctt ttgtatctcc tggctggggg gctttttggc
    36301 tgtgctcatc ccaaacgggc tgggcgtacg cgaaacggtc ttgaacgcgc ttttggagtc
    36361 gcaggtgggc tttgctgccg cggcggcagt cgtggtttcc gtggcttcgc gcgttttgct
    36421 cttcctggca gagctggtct ggctgctgca tgcgctggtc acaaagcaag attcacccgc
    36481 tgacctaaaa tactcctaag gtattattgc gcgcctgagg ggcttcccgt aagatatatg
    36541 caagaacaaa gatcgtggag gatcctatga aaaaatactt cagtgttgta cttgtttgtc
    36601 ttcttgtctt tctgttagga gtagcacccg tcagccaaag ccaccagatg gcgattgctg
    36661 cagccaccac caagagcttc tcatccaact acacgcttgt gaacctgggt ggggatacag
    36721 ccgcggtgac cgccagctat atcgctccga acggcactga atggggcagc tcaatcttca
    36781 agtccttctc cattcctgca ggcgccaacc agatcgtccg tcagtatgac gacaccggtc
    36841 tgacccccgg cagaggttcc gtggtgattg cttcatcgca gcctctcggt gcgctggttc
    36901 aggaaattgt ccgtactggt gtgccttctt ccggcgctta cacgggtatc gatgtcccca
    36961 gctcaatctg gtatgttccc gaggtctccc gcatgggcaa ttcggctacc ggcactgcca
    37021 acagccagat tgtcgtccag aacgtgggta ccgcagctgt tgatttcacc atcgcgttga
    37081 ctgcttttgg cgccgcaacc cccacgtaca ccaaatcctt caccggcctg gcggctggcg
    37141 cttctatcct gtatgacatc caggaagaaa ccaacctgcc taccggcttc tggggctctg
    37201 cggttgtgtc cactgccagc ggttccctcg gcgtagtctc acacctgttc ttcggcgcgg
    37261 acagcctgat ggccttcaac ggctttgctg ctgctgatga ctcctggtac gtcccgctgc
    37321 tctacgtccg cctgggcaac tccctgacca cctctctggc tattcagaac ctgagcggcg
    37381 acaccatccc ggtgaacgac ctgaccctgg cctgcaccaa gaaccctgcg gctcccggcg
    37441 ctgccacaat cacgctgcac aaccacactg cgattgccaa caacggcgcg tatactttca
    37501 acaccctgac cgatacgacc cgcttccccg atgccggctg gtatggcagc tgcaagatca
    37561 gctctgccac ggctaaagac accgtcacac tggtcatgta ccgctatgtt gccaatgccg
    37621 aacaggctgc ttacggcgca gttcccggca gcctcgccag caccaaggtg tctgttccat
    37681 tggttgccaa gcgcttggcg aacggttacg caaacgtcgt caccgtacag aacctgagca
    37741 ccagcgccgc gaacgtaacc atcacctata cccctgctgg cggcggaacg cccatcgtcc
    37801 gcactggcct cacgattgct gccggagaca gcctcatccg caacttccgc ctgagcggaa
    37861 ctgagagccc tgacatcagc gacggctggg ttggctcgat gtccgtatcc gctgatcagc
    37921 caatcgctgc ctacgtcgcg aacacctacc tgaaccccag cggtgaccag ttcatggcct
    37981 acctgggctt caactactaa accttaacct gtttgacgga aaccctcacg gagaagaccc
    38041 cgtgagggtt tataatttaa atacctatga aaaagacatt tcttcccctc ctgcttgtcc
    38101 tgtcgctgat actggcagcc tgctcttcca atacgcccgc cccaacacag cctgaagcga
    38161 gcccttccac tgccctgcca gaagtaacgc aaagctcgcc cgagacggct taccccgccg
    38221 cggagatgcg actgagcagc gcttatccaa tcaaactgcc ggaaggcgtt ccgctcagca
    38281 gcctttccgc cccaactgaa gcgctgccgc cccaggaagg cttcgcctct atcagcgggc
    38341 tgctgtatgc ctacgatatc tcgcaaattc tggtggatac tgacttctgg ctcttcccgg
    38401 ccgtgaccgt tggggaccag aaagtgcccc cgcccatcat caccaatggc gacccggctg
    38461 ccggggatat ctacggacgg acggacgcga acggcgcgtt ctatctcgat aacgtgcccg
    38521 ccggggatta cttcctgctc atcaactacc cggaccacac cgtcttcggg caatccagcc
    38581 ccacgccgga cgcgtacctg ctcatttcag tcgccgcggg ggaccggatt gcgtacgggg
    38641 tcatctatac gctgcgctaa gaatcagggg tttgtcagga agggaataat cagagagtaa
    38701 atcgcctgat aacggataag ttctgcgctc aagctgccgg tttcgtccat cccgcccaaa
    38761 atatagaggg cgttcccata tcccgtcagg ctttggtctg tgcgcgggct tgagacgggc
    38821 atgccggggt ccgacagcca actgccgtcc ttgggattgt actgccagat cgtactttca
    38881 gacaccgcga aaataaagtc gttcaaggcg gcgcctgtgc agcgcgcgca cttgaagggc
    38941 gttttcgctt ctgcctgcca cgccagttcc ccgccaagcg gggtagtcca gagcgcatcc
    39001 agggtttttg agccgttggt tcccccgaac aagtacaatc tgccgccgac gagcgcggac
    39061 cccatggatt cccgcgcgcc gggcatactg ccggcagtgg tccatctgtc ctcggctggg
    39121 ctgtagcgca agacggcgtc cagggtctgg ctgccgtccc acccgccgaa aaggtacagg
    39181 ctgccgccgc ttacccccag cgcgtaggcg ctgaccgggt aaggcaggtc cgctttgcgt
    39241 tcccaacggt tgctcagcgg gtcgtagact tccagcgcgc tgctaagttc tccggatgcc
    39301 agccggccgc cggggacgta gattttaccg tccagcagcg cggcggagac gtccgcggca
    39361 ggcaggggct ttggttcgcc cgcactccag gcgtcctgct gcgggtcgta gatatccacg
    39421 cggcctgaaa cgccctccgc gctgagtccg ccaatcgcga aaagcttgtc gccggcattg
    39481 atcaacaccg cgccggcagt ttctgtggga atggacgcca tcacctgcca gcgctgttcc
    39541 attaatgcgg aaagcaccgg gttgggtgtg gcggtggggg gcgagaagag cggcgtggaa
    39601 gccagcgcga aggaaagccc gatgacagcc gccagcgcca ggagcgccag acccggccag
    39661 tacttccgca ggaatgcctg caagcgggtc ggttccgctt cgggtgcctg gattggttcg
    39721 ggcaccagaa cttccacttc cccgttggct ccggcaggag atttgacgat gccccgctct
    39781 atcgcgtaaa gcgtggcttc cgtgcgggaa gctaccccga ttttagcgaa aatattgccc
    39841 aggtggactt tgactgtatt cacgctgata aaaagttccg cagcaatatc cttgttgctc
    39901 tttccctgac caacaagctg cagaatctca atctcccgat cgctgagggg caatttttcc
    39961 ataaggtcac tttacctgcc tgtaaacctg caggcaggca ttattataca ggttttatcc
    40021 cccccgacgt tttttacacg cgcgttggga gatgcgcaga cggctgtctc tatttgtaga
    40081 gcaaaataat attgtaggtg tgcgatgcag gtgtgtacgg attcccgctg gtgtagtagg
    40141 tgcgtccggt tccgccgtac caggaagcct ccgaggccag cctggggcgc ccgccctggg
    40201 tggcggcgtc ccaattgatg tccacatccc ggaaggtgat atccacggat ttgccccagc
    40261 agtaattggt gcaggcgttc agggtacttt gttccacgac cataaagtgc aggtggtggc
    40321 cggtgctgta gcctgtgtcg tccgctaagg caatggtctg accctgaaaa accggcacgc
    40381 cgaacttgcg caggttctcc ggcacgctgc ccttggcaat gtgcatgtag atgtgatagg
    40441 tgtaggggga ggtggagcgg tcctgaatgg tgatgctgtt ggtgcaggtg gagtctccgt
    40501 tgttgcagct gtctttaaag tggtacacat agccggactt ggccgcggta atctcgaaca
    40561 tcgtgccgtc cgcgaagtca aaggcgtaaa ggcagtagtt attcgcgcag gaattgtgcc
    40621 cgaccgacca ggtgaggcgt ttggtcagtc ccccgcgcca gggcagtttg aagccgccgt
    40681 aaatcgtgcc tgtgacagac gctttcgggt cgtattccgt gaaccgcccg taaatttccg
    40741 agtcccggaa atcggattca ttcagcttgt ccaagaagac atcgtcgtct ttctgataca
    40801 aattccactg tccggtatcc tcattccaga gcgccaggat gacctctggt tcgcgcgcca
    40861 gcagctcgcc ggtatccggg tcgctggctg tcagccacaa gactgcctgg tccttgacct
    40921 cgttgaaggt gatgtcgtcc agcttcaggc cgtcgaggcc gcgttccgcc agcgcgcccg
    40981 gcagctgcgc gaagagcagg ctgtccagcg ccgtaccttc accaggcggg ttcttggagc
    41041 cgctcttgtt tccatcggag aaaataagca caaggctcag cacaaataag agcgccgcgg
    41101 caataaacag gaatttgtag cttttttttg ggcgttcttc cgggtcggta gctggcgtaa
    41161 aggtttgatt ttgcataggt caccacgcaa tagcttatat ccttggtttc aagggaacat
    41221 ggtgcaattt atgtgccagt gcttttggca ggcaatattt cctgtcaggg ctggatagaa
    41281 atttccggat gtgtgcagga agttggcagg aatggttttt cctcgaaaat aagctcctta
    41341 attcttcttt tttatcatgt tccatagttg ctctgaccct gacccttccc cagcggcagg
    41401 cggctcattt tttgcctcgc tcaagccgcc ggggaaggga aaaatgtttg agaggctgta
    41461 caaaaaggcg gacgaccctt tttggccagt ccaaagtgag aatacgggcc tggtttcggc
    41521 ctcgttttgg tagcgcaaat ccgcaaacgc acagtaacac gggcacggag cgggaaatca
    41581 aaaaaggctg tccaactttt gggacggccc caaggattac ctccccatcc cagcgacggg
    41641 aaattcggat agtagaataa gcttcgttcg ctgaagaaag ggaggatgag gggaaaggct
    41701 gttattcgct tgtatgtcag gggatgacta ttccttttat aaaaacagaa ggaatcgaat
    41761 gctgattttc tggctcctga tcttcatcag gatatttctg ttttgctatt ccaggacatt
    41821 gttccattgc tcttgcagca ggcaatattc agctccgtcc gcggagaagc cggctaaaat
    41881 aagttcagga taagcggaaa aagaaaagct gatttggata tgatgaatca aaagattgat
    41941 cggctcagcg aggttgtact ccgggaaatt ggcggcagga tggttggaaa gcggcttgtc
    42001 ccactgctgc gcctgctctt cagccggcaa gcgcgggaat tctccaggct gctgctcgcg
    42061 ttggaagacg ggctggactg cccgcagccc tggctgaccg cccgcgcgac cgcatccgcc
    42121 ctttccggcg gcctggaaat acgcgctcag gagcgcgtgc ccgcggaagg cccgctgttg
    42181 gcgcttgcca atcaccccgg catggcggac gcgattgctg cctttggctg cctgccgcgc
    42241 gcggacaccc tggtcgtggc aggcaggacg cctattctga ccgggctgcc gaatatcagc
    42301 cggcgcctga tttacctgcc cgaaaataag gcgggcggca tggtggtcct gcgtgcaatg
    42361 gtggactggc tgcgcaaggg tggggcggtg ttttacttcc cgtacggcct attggaaccg
    42421 gacccggcgc gcgtccccgg ggctgccaac accctgcagg cctggtccgg gagcataggg
    42481 gcggtccttt ccagagcgcc tgaaacaaag ctgctgccga tgctcatcag cggagtgctc
    42541 agcaagcgcg cgtgggagtc cgggctgctg agccaggtgg tcaaaaccaa gcgccggcat
    42601 cagctggcga tgatctggca gttcatccgg cgcgtaggtg cgcacggaag ggattgggac
    42661 agcgtaatgc gcgtagatat tggacaatcg ctgccggcgc gcagcttatc cgcccggctg
    42721 gacccggcag aaatcaatgc ggcagcccgc gcggccttcg gcggtctgct aaccgatact
    42781 tttcccgaag atcccggcat gctgccggtc tgcgactatg taaccccggc ctaatcgagc
    42841 cattccacga gcgcggcttc gccctgcccc acgcccacgc acagggctgc cagcccgtaa
    42901 aacgggcgtt tggcttccgg cgcccggcgg cgcatttcgt gcaccaaagt ggtaaggatg
    42961 cgcgcgcccg agcagcccag cgggtggccc agcgcgaccg ctccgccgtt cacgttggtc
    43021 agcgcgtggt tcaaccccag ttcccgcatg actgccagag cctgcaccgc gaaggcttcg
    43081 ttgatttccg ccagggagat gtcctcaatt gtcaggccgg cttttttgag ggcgcgtcgg
    43141 gtcgcttcga tgggccccag gcccatcacg cgtggttcaa tcccggaggc tgcgccgctg
    43201 accacgcggg cgagcggctt gatcccgagc gcgcgggctt tttccgcgct catcagcacc
    43261 agcgcggcgg cgccgtcgtt cagaccggaa gaattgcccg cggtgacgct gccgtcgggt
    43321 ttgaaggcgg gttttagctt gccgagagct tccaggctgg tatcgcggcg cggacgttcg
    43381 tccaggctga cgagaagcgg ctcccctttg cgctgcggaa tggtcacggg caggatttct
    43441 tcggcgaact taccctggtc ctgggcggct actgcgcgct gatggctgag gaaggcgaat
    43501 tcgtcctgct cctggcgggt aatgccggtt tgcgcggcaa tgttttcggc ggtcaggccc
    43561 atcgcctccg tgccgtagag cgcttccatg cgcgggttga tgaagcgcca gcccagcgcc
    43621 gtgtcgtagg cggtcagatt gcccccactc aaggttttgg cgaaggcgta gggcgcgcgg
    43681 ctcatgcttt ccacgccgcc cgccacgaag atttcgccgt cccccgcgcg gatcgcccgg
    43741 gccgccaggt tcaccgccgc cagccccgaa gcgcacaggc ggttgaccgt cagggcgggc
    43801 acctcgggcg ggagcccggc cagcagcgcg gccatgcgcg ccacgttgcg gttgtcctcg
    43861 ccggcctggt tggcgcagcc gaaaatgact tcgtcaatct cgtgtgggtc aatgcctgtg
    43921 ctccgcacga cctcggcgat gaccagcgcg gccaggtcgt ccgggcggac ggtggaaagc
    43981 gcgccgttca ggtttccgat gggggtgcgc agggcttgga cgatgacagc ttcgttcacc
    44041 agggggctcc tttggatcgg gattatctga attttaccat caggcgggta tgcgcttgcg
    44101 cagagctctt gatattaccc agacattgag taatacccag gttaataaag atatctgttt
    44161 tgactctttt aagacggaaa aaatatataa caatccatga tgtttcaagg ccaaatcatc
    44221 atggaatgat aataatttcc tggactattc cttggaaggc agcgtaatca taaatatcct
    44281 tgctccaatg atcggtgagt aagacactta caagttggcc gggcttaaga gcatcttctg
    44341 tcaccgataa gtatcctgtc gctgtttgct gccagaggag agtgtaatat gcaagcgcaa
    44401 gcctcgtctc gttcaggtcg ggatatttgg gggaactcgt gacatgcaag tcaatcagct
    44461 tcgtcgtgtt aatgtgaaca tactcttcga tggaggtgat agtgcctgta aattctgggg
    44521 agagaaatgt agagatgggc gtactgcttt ctacggcgcc tggggtcaga atgagcacct
    44581 cagtagcttg ggcctggaac ggatgtgatt cgcggatgta ctcagcatta aacttaatcc
    44641 tctgtccaat tgctaagtct gataagcctg ccagctgaat ttcttgacct tcaaccttcc
    44701 aaacaaggca atcttcagtg aagctaattc gtcctggagt agatatttca tcagattgct
    44761 ctttaatctc accaaagtcg ataaccttac tttcgtacgg cagcaaattt aatactacgc
    44821 cctccattaa ccaggtatgg ttggagagg
//
LOCUS       DAOQAD010000006        41195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_820_length_41195_cov_6.240715, whole genome shotgun sequence.
ACCESSION   DAOQAD010000006 DAOQAD010000000
VERSION     DAOQAD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 41195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41195
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_820_length_41195_cov_6.240715"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(455..1351)
                     /locus_tag="PKL60_01090"
     CDS             complement(455..1351)
                     /locus_tag="PKL60_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HNW94760.1"
                     /translation="MRALFEDRFVLDDLKSLGAGVALVVRDFSEERAQAARLLNKLGI
                     PVSAWLLLPGEQGTWLDRADSAQAAELYQALIHWSGVKGLRWSAIGYGVPEREERAAQ
                     KSTNGLTASAAISTLIERAHADGFAVELVYSLPQGAPSQIKNLGLGRKTMPEVPKAER
                     CIHLPPNINETSRAFREGGADGKGAVRVVNARKSLNLGTSTDEEPKTWQEFARELCAA
                     RQQSSAVYVITLESCANQGFLDRLVTLDWNGQARSGQEIPNAAVRRGPTGGQKSRWLG
                     IATMAGFVGAALLVTRKKKRKD"
     gene            complement(1470..2186)
                     /gene="rsmG"
                     /locus_tag="PKL60_01095"
     CDS             complement(1470..2186)
                     /gene="rsmG"
                     /locus_tag="PKL60_01095"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNW94761.1"
                     /translation="METFTQNARQLLGLQLTREQILAFKLLEDELLEWNSRFNLTAIR
                     DKEGIETKHFLDSLTCLMALDASASPRSLIDIGTGAGFPGIPLKLILPQLRLTLVESV
                     QKKANFCAHIVEKLGLRQVQVLAERAEEVGQDPNHRESYDLATARAVAAMPTLVEYLL
                     PLVRIGGLVIMQKGESAQLEALKSEKVIQRLGGKIRVILPVVLPGVADERYLVVLEKV
                     ARTSAEFPRRTGLPAKQPLG"
     gene            complement(2250..2813)
                     /locus_tag="PKL60_01100"
     CDS             complement(2250..2813)
                     /locus_tag="PKL60_01100"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNW94762.1"
                     /translation="MTQASQPGKTGFTRTQVLWRVLLLLVLIAVTALIIIFRTQVQKL
                     QAFGYPGIFLFSVLSNATILFPLPGVVFTSAMGTVFHPAWVALAAGLGAAVGELSGYL
                     AGVSGQLLVENKDNYQKVLGWMRKYGLWTILVLAFIPNPLFDIAGFIAGSLKVPAWKF
                     LLFCAVGKIAKMLLFAYLGAGLFSLFA"
     gene            complement(2788..3435)
                     /gene="udk"
                     /locus_tag="PKL60_01105"
     CDS             complement(2788..3435)
                     /gene="udk"
                     /locus_tag="PKL60_01105"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNW94763.1"
                     /translation="MDTGRSPLVIGIAGASGSGKTTLAFSILDAIGRDKIAFLPHDAY
                     YRDQAELSYAERLKINYDHPDSLETDLLIRHIVALKAGKAVRRPVYDYKQHTRSAEVV
                     MIEPCQIIMVEGILIFWEAKLRKIFDMKVFVETDSDICFIRRLTRDIEERGRTVESVV
                     NQYLTTVRPSYIDFVEPSKRFADVIIPEGGKNTVALDMVIARLQGLLNDASQSAG"
     gene            3493..4377
                     /gene="folD"
                     /locus_tag="PKL60_01110"
     CDS             3493..4377
                     /gene="folD"
                     /locus_tag="PKL60_01110"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNW94764.1"
                     /translation="MTAHLIDGKQIAAEIRGDVKLKVDERIQRGLPIPGLATVLAGEN
                     PASQVYVRNKQKSCAEVGIRSFGHTLPADASQEEVEALVHKLNADPEVNGILVQLPLP
                     AHLNEERVLSAIDLSKDVDGFHPLNIGRLAQKGREPLFIPCTPSGVMVLLEKAGARLS
                     GANAVVLGRSNIVGMPVALLLVRADATVTICHSKTRDLPTLVRQADVLVAAIGRPEMV
                     RGDWLKPGAIVIDVGINRVEDASAPKGSRLVGDVAFEEALDVASAITPVPGGVGPMTI
                     AMLLQNTLRARELADGAA"
     gene            4403..5788
                     /locus_tag="PKL60_01115"
     CDS             4403..5788
                     /locus_tag="PKL60_01115"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HNW94765.1"
                     /translation="MPTLLVKNAALIVTMDAQERELQDAAVFCRDGFIEQIGAAADLP
                     SEADEVLDLAGHIVLPGLVNTHHHFYQTLTRALPAAQDANLFNWLKTLYPIWARMDPD
                     AVFLSTQTALAELALSGCTTTSDHLYLFPNGSRLDDEIDAAAEIGLRLHASRGSMSLG
                     ESKGGLPPDSVVDSEDAILRDSQRLIERYHDPKPGSRLQIVLAPCSPFSVTGELMRES
                     ARLAREYGVHLHTHLAETEDEDDFCLQKFGYKPVAYMQTVDWIGSDVWFAHSVHVNAA
                     EIDLYARHGCGVAHCPSSNMRLASGIAPVMQMLSRGVKLGLGVDGSASNDSSHMLAEA
                     RQAMLLSRLNSGVHGASRSAPDAPPLMTARQALSIATRGGAAVLGRQDIGSLEPGKCA
                     DFFAINLNRLEYTGAWQDPLAAVVFCSPVQADWTVVGGRPVVKEGRLLSLDLPRHIEK
                     HNAASRKMILG"
     gene            5818..6765
                     /gene="arcC"
                     /locus_tag="PKL60_01120"
     CDS             5818..6765
                     /gene="arcC"
                     /locus_tag="PKL60_01120"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNW94766.1"
                     /translation="MEKKGLAVVAVGGNALIKDKSHQTVPDQYQAAADTMVHIATMIE
                     EGWDVVVTHGNGPQVGFILRRSEIAAHELHEVPLDYCGADTQGAIGYMFQQALYNEFL
                     KRGIRKSAATVVTQTVVDSEDPAFKNPTKPIGSFLDEAQAKVKMEKDGWTMVEDAGRG
                     WRRVVPSPIPQVIVEADAIRSLIDQGFVVIAVGGGGIPVIRTPEGKLVGVEAVIDKDF
                     GSAILACMIKADLFLISTAVEKVAINFNKPDQKWLDRITAAEARQYLKEGHFAKGSML
                     PKIEAILKYLDCGGKAALITDPEHIKDALDGKTGTWILP"
     gene            6805..8049
                     /gene="thrC"
                     /locus_tag="PKL60_01125"
     CDS             6805..8049
                     /gene="thrC"
                     /locus_tag="PKL60_01125"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNW94767.1"
                     /translation="MRISHYTCSVCGAEFSETETQYVCPRDGGNLTVHLDFETAAVRP
                     NPNDILESREASIWRYLPLLPVEDPGYHNTPLRQVGWTPVYRPQTLQQELGLHRLWIK
                     DESRNPSASLKDRASALVTARMKMTNQEVVVAASTGNAGAATACMAASAGLRAIIFAP
                     RTAPQAKVAQLLSYGARVLLVDGSYDQAFDLSLAASDTFGWYNRNTGHNPFTAEGKKT
                     AAFEIWEQVLRHLPAESEALRVYIPVGDGNIISGIHKGFQDLLALGWIERLPVLIGVQ
                     AEKSAAIANAFFTKADAITPVQATTLADSISVDLPRDGLRALQAVRATHGHYVTVPDE
                     EILMAIPELGRAGIFAEPAAAAAFAGLRKDLREGQLNPDSPTLVLCTGSGLKDARAAI
                     SAVPSAPIIEPTIQALMKVIHV"
     gene            8042..9028
                     /locus_tag="PKL60_01130"
     CDS             8042..9028
                     /locus_tag="PKL60_01130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW94768.1"
                     /translation="MSEPVFSIIAPIFNELENLPLLYARVREELEKTGQEWELIMVDD
                     GSKDGSTELIRKLAAADERVRPVIFARNFGHQIAVSAGLDYSRGAAVIVIDSDLQDPP
                     EVIGELIAKWREGFEVVYAVRTEREGETKFKTQTASLFYRMIDRITDIKIPLDTGDFR
                     LMDRKVVNIMVAMREHNRFLRGMSAWTGFRQVGVEYKRKARHAGVTKYPLKKMLRLAM
                     NAVTGFSMWPLQLATRLGLILGALGVLAIPVVIVLRYAADVQFHGLATLAILMTLLSA
                     LILVFLGFIGEYLGRVYDEAKNRPLYIVAEGPREPFNPVRLDLPAETKSNHS"
     gene            9043..10506
                     /locus_tag="PKL60_01135"
     CDS             9043..10506
                     /locus_tag="PKL60_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNW94769.1"
                     /translation="MTKIDLTLMPENLQRTTQRVREKRFYIPTLAQQKDPALIPAHIK
                     EQLKTVGLWDLDPLNLFRITWHNEARASGGLFGNVNYVEFPPELTGVPARVVALVGKW
                     FPTGCHKVGAAFGCLVPRLVTGQFDPTRQKAVWPSTGNYCRGGAYDSYLLGCESIAIL
                     PEGMSRERFEWLSRIASEVIPTPGSESNVKEIFDKCWELRRSGQDLVIFNQFEEFGNY
                     LWHYTITGSAVEEVVAQLSRPGDAYRGFVSATGSAGTIAAGDYLKQRFPTSKIVASEA
                     QQCPTLLTNGFGAHRIEGIGDKHVPWIHNVRNTDFVTAIDDQAVVNLARLFNEPAGRE
                     HLINSGVDETLVNQLDLLGFSGISNLVSAIKFAKYNELTSRDIVVTVLTDSMELYRSR
                     LTEMQEQYGQYSPADAIADDARYLKGQPIDFMQELSYYDRKRIHNLKYYTWVEQQGRT
                     YEEIQAQWYDPDYWLEIQAQAPEIDARIEEFNRAAAA"
     gene            10516..11091
                     /locus_tag="PKL60_01140"
     CDS             10516..11091
                     /locus_tag="PKL60_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94770.1"
                     /translation="MNPKSTAPPDTALVLVAIARDQRDLEIARVLGWYRIPLKSAPKV
                     ISVDYLAFYQTSAFPTAERWQIRWIAPILGHELTTRAELLRDQPDHPRAPEEYFKLQL
                     GPLQELAQPIPADTWKRVTFFYTTFGRVRQAAVLADLPVHDEERAVLWQSLRERALRA
                     NEYQAAELPQMPVDPAILALFSGGFRPTHPE"
     gene            11149..12564
                     /gene="hydA"
                     /locus_tag="PKL60_01145"
     CDS             11149..12564
                     /gene="hydA"
                     /locus_tag="PKL60_01145"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HNW94771.1"
                     /translation="MRKLIKNGTVVNAESSQQADILVEGEKITRVEPGLDCPDAEVID
                     AAGMLILPGGIDPHVHINLPMAGTTSSDDYYTAGKAAAFGGTTSLIDFVSQDEGSLAE
                     NIARKLQESDGRASVDFGLHMNITRFTPGVLDEIASLPELGITSVKVFTAYNQRLRLG
                     DGEIFQTMRAAAKHGLLTLVHAENGDVIDVLVAEALQQGHTSPVWHAYTRPAWGEVEA
                     SLRAMALAAQADAPLYLVHMNVAGEVDQLAYGRAHGVRAMGETCPQYLFFTAQDLERP
                     DGTKWLCSPPLRSPADQQGLWQGIDNGTIQVLATDHCPFFYDGTRAISYEDQQVALPG
                     KELGKGIFTQVPNGVPGVGDRLPVFWTHAVHSGRISPSQFAALTSANAARIFGLYPRK
                     GCLAAGSDADIAIWDPEMRVNYGAQTAKHRTDYNLYEGWQLTGFPRQVFLRGECLVRG
                     GEWFGRPGQGRFLRRQSGEII"
     gene            12497..14197
                     /locus_tag="PKL60_01150"
     CDS             12497..14197
                     /locus_tag="PKL60_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94772.1"
                     /translation="MENGSGALARADSCAAKAEKSFDQFSTRQIVVIPLLILLLLAAS
                     AAITVSHALKPKVARAWMIALISALVVWFGFFILRLYLPLSFALPAWKPEALFISAPS
                     LELNYQNWPYAVALVTLCTAVILTDSTRSFLPSSPNAWANAIAITAVNLIGILAGDPI
                     TLMFAWVIIDTIEFVYLYQTRLDRSSDAGLAGFMTIRVLSVMALAAGAVTGWLSMPAF
                     GMAEIPQKAGIYFLMAAGLRLGVLPLNLPVQNSSEPKHGPALLTRLAPVASSLALIAH
                     LPQGLLTSQSALRPLFLALTAAAALYASVMWLSGKNQHSALPYWIITLAAFAITCALN
                     DRAEASRVWGVALLLSGGALFLFDPPIRRIRFLPLLGLIGLSALPYTLAAGGWDGLLA
                     DKFTFSTGLTLLSHALLLLGYLRYTFEISGTVTGLEKHARLTYPLGLILIVQTIVIIG
                     LAGWPGVLTAGKWWASLISLALTGLGAGLYLKRGAKLPLASAAASLPLYRFWTFLLKS
                     FQQFLSLQWLYKAIAWLIGQFAALAGLFNQVLDGEGGILWSLVFLAVLITLFLSGVNP
                     "
     gene            14194..14796
                     /locus_tag="PKL60_01155"
     CDS             14194..14796
                     /locus_tag="PKL60_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94773.1"
                     /translation="MTGASWMYIPAVLAIAAAGINLTRRLWVVNAAALGLQYLGVFVL
                     LTTVRPASLALVKLLVGWMVTLTLFLSLITDGKIRSAKAFFSFSAGEIFRLLAGVFAV
                     FFLAALLPQLQASVFPQTSLPLLLAAFSLLVLGLFQASLSAEPFYVIIGLLTFLSGVE
                     LLYASLELSILLEALFAGVNLGLALVGAFFVIKDAEIPEA"
     gene            14793..16211
                     /locus_tag="PKL60_01160"
     CDS             14793..16211
                     /locus_tag="PKL60_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNW94774.1"
                     /translation="MSTPLIWVVFPLGFALLLIITRKKPMLSGILASVFCLLLALAAL
                     LFPRDLSFLLPDQRLEIASTLELFGRSLQINGNQLTIIGLLYGIAFFWNLGSLLYRPS
                     LWFNILSLGITALWVATLSVNPFLYAAVFIELIALLSVPLLSPRGVRTEHGVLRYLVF
                     QTLALPLILLTGWMLAGIGSAPSANPLIVRSAMLVLFGFALWIGAFPFHSWIPMISAK
                     SHPWVVSLLLTLLQSALSVFLLHFLDQYAWLRNLPVTFASLRWIGVTMIAIAGLFSAV
                     QTNLGKNFGYLVLYETGYSLLAIGLAQSGGLNYLAMLFIPRVLSYALLSLALSALWEK
                     GNAEALAYKAQEGLFKRRPVVSAGILAAFFSLLGLPLLPVFPHKQMLWLLTAQSDPRL
                     LVWVLVGSLGLLLSIARLLRLFAREGSAADEAGPTREKGGLIALMLLILLLMLAMGIT
                     TQLLLPRFLDILSPFTQLAPGL"
     gene            16272..17465
                     /locus_tag="PKL60_01165"
     CDS             16272..17465
                     /locus_tag="PKL60_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94775.1"
                     /translation="MDDITSFEQLEKRVQWLDNERRNDKTNLASLQNRLTGLESENIS
                     LHKQIKDLELELSGLKNQISSLDKYENRIERLNIDLTKQVRDVNERAEMNLNEAVKRL
                     KLEIDATKRSLSEIYQIGEQLEPIRNELKSHKVEDARLARLIEELKGKIQEVSRFDED
                     YRRSLHLLEENYRQENKRLNDFQGDLAAHRKRVEDTRSRFDVFSDSFRTLDVRITELQ
                     TFEKDRKEAQSVFIEKVNNSLLDKEKTFRVWEKRFGEIDQVKLDLNTQLADLENARKA
                     LDKSISSSEEVVARFERRINEISEIQRLNDERFRQEWNSFKADDLKRWTNYLLGQEEQ
                     LRTINQQLSSAHEELEALKDSSVKIQDELDKLTRETFRSIQSILGAYQESIQSLAPLA
                     EKKAQ"
     gene            17470..19365
                     /gene="selB"
                     /locus_tag="PKL60_01170"
     CDS             17470..19365
                     /gene="selB"
                     /locus_tag="PKL60_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNW94776.1"
                     /translation="MHIVGTAGHVDHGKSTLVTALTGINPDRLKEEREREMTIDLGFA
                     SLTLPNGEKVGIIDVPGHRDFIGNMLAGIGGIDAVLLIIAADEGVSAQTREHLAILDL
                     LHITCGLVVLTKLDLVSDPDWLELVELEARETLKGTSLEDAPVLRVSARTGAGIPELL
                     EKLQEVLDHTPSKRDLGRPRLPVDRVFTLTGFGTVVTGTLLDGSLAVGDEVVSLPEGL
                     SARVRGLQNHNQKLQKLAPGARAAVNLVGIEKNQIARGSVITKPGAYTPTTLLDIHFR
                     YLADSSFELRHNTQVKIYIGAAEAAGQLRLLGKEQLKPGEQAFLQIRLASPVVAARGD
                     RLIIRLPSPPETLGGGVVLDPHPARLHKRFSEPVLRNLEGLLSGGESEALLQALKALR
                     AASSEEVLARAGMDRQASLALLDGLISSGSIIPLQTVKEPSRNLLMKAETWQQVRAEL
                     LGALAEFHAGSPLKPGIARELLRGACKLPTQLFDAALEKLREDQEVTVQGALAALSSH
                     RIAYSPDQQAQISMLLRRFEESPYSPPDAPEAARLIGAPLLEALVATGTLVQVSPQVL
                     FSPQALAEMSAWVEASIKEQGSLTLAEFRDHFSTSRKYAAALLEYLDENGITLRKGDI
                     RVLKQVQ"
     gene            19405..20343
                     /locus_tag="PKL60_01175"
     CDS             19405..20343
                     /locus_tag="PKL60_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HNW94777.1"
                     /translation="MAWKVLITDGLESNGKAILTQSDIAVDKKGIEAAELLQIIPEYD
                     AIILRGRTKMTAEVIAAAKNLKVIGRMGVGVDNIDLNAAKAHNVTVVNAAVATSLAVA
                     ELAVGMMFALAREIPRADASLKAGQWLKKDFNGMELFGKTLGVVGFGNIGQVVSRYAL
                     ALGMQVLSYDPYRDTMYMHERGVEAVSLDGLLERADVITIHTPLTDQNRHQFGDACFS
                     SMKDGVLIVDAARGGIIDEEALLRALESGKVRGAALDVYEKEPPVSFDLIKHPRVIAT
                     PHIGAQTSEAQLRAAEDISHEVLNALNGLPLRWKIC"
     gene            20347..21855
                     /locus_tag="PKL60_01180"
     CDS             20347..21855
                     /locus_tag="PKL60_01180"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNW94778.1"
                     /translation="MGSEEKLAKLREITAEIRRLGSIQALLEWDQQVNMPRGGAEDRG
                     NQTALVAGLAFDKSTSDELGALIEDLAAEVPDLDLDDDFAREIKVAKRYYDRNARIPR
                     EKIIEFVLATSAAHEAWVKARAENDFASFEPHLTHIFDLRRQQAELFQPYEHPYDPLL
                     DEYEPGMKTADVRAIFSALRPRQVELLRAISESEQVDNSFVKQHYPFEEQRKISSFLA
                     GRFGYDWERGRLDLAPHPFTTSFGHGDVRITTRFLDMDGMSALFSTMHETGHALYDQG
                     YSPRYRETSLDGAASLAMHESQSRLWENLVGRSQEFCRFVFPVMQMLFPQHLGNVSLE
                     QFYRGINRVEPSLIRVEADEATYNMHIMLRFEIETGLLEGSLRARDLPEIWNTRMQEY
                     LGITPADDTTGVLQDVHWSSGLVGYFPTYALGNLISAQLWEKMVAAHPEIPGAMSAGD
                     FSQILAWLRENVHQHASRYDPQDLVRKITGGPIDPEPYLRYLNAKYSRIYRF"
     gene            complement(21936..23117)
                     /locus_tag="PKL60_01185"
     CDS             complement(21936..23117)
                     /locus_tag="PKL60_01185"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNW94779.1"
                     /translation="MKILRNMSFLLLVPALLSGFLPAGTARAASAETDGSYYDLPLCL
                     PGFYPSAPDDCLALGASQSVKNLADEGIPDILEELPAASPDYSLTELPVSVAKIVAGT
                     SEAVPVYASLDEARNGGTPLRTLAAGPMRYVSMVNQVSDGGRILVQLESGEWMQAQPI
                     YSWTRFQGLRFSRTPRNDFGWIVNDAESYTAPGYASPKTGKVHAKYEVIQIYQVAEAD
                     GYEWYRINQVEWVSSHKSRRMVVDTTRPEGVTGDRWIHIDLGNFTLSVYEGGELVFAS
                     LIASGLPPFYTQPGTFQIYKMYETVTMQDAYEADRSDFYHLQAVPWAMFYDRNTAIHG
                     VYWPVVLGYNQSHGCVNMSPGDANWVYHWAKVGDWVYVNDPSGKTPTDPAFYGGVSTG
                     P"
     gene            23310..24110
                     /locus_tag="PKL60_01190"
     CDS             23310..24110
                     /locus_tag="PKL60_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNW94780.1"
                     /translation="MKIPVYHVDAFAVEPFRGNPAAVCLLQAPRSVEWMQNVAAEMAL
                     SETAFLVPKGEGYYLRWFTPKTEVDLCGHATLASAHILYEFGFYEPDETIKFQTQSGI
                     ITASFLRGTIELDMPRRDPSPAQVSAQLSEVLDQKPIASAVYKNQVILAELENAEAVR
                     NFVPDFKKIASLPYPDVIITAPCKEKYDFVSRFFSPLTGIPEDPVTGMAHCTLAPYYA
                     ERTHKTKFFAYQASARGGEVWVRLGSDRVYIGGKAVTVSQGDLLHQKD"
     gene            24230..24802
                     /gene="xpt"
                     /locus_tag="PKL60_01195"
     CDS             24230..24802
                     /gene="xpt"
                     /locus_tag="PKL60_01195"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNW94781.1"
                     /translation="MELLKQRILQDGKNLGSGILKVDRFINHQVDPLLMDACGAEFAR
                     LFAGLGAEKVLTAEISGIAPALMTGKYLHLPVVYARKTKPITMPNEVYLTVAPSHTKG
                     NNVELIVSPEYLGREEHILIIDDFLASGATIAGLVRLAQVAGAQVVGIGTLIEKSFEG
                     GRRLLSYLNIPIHSLVTITSMDAGKIVFAD"
     gene            24819..26381
                     /gene="guaA"
                     /locus_tag="PKL60_01200"
     CDS             24819..26381
                     /gene="guaA"
                     /locus_tag="PKL60_01200"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNW94782.1"
                     /translation="MTDKIIIVDYGSQYTQLIARRVREAQVYSEILAWNDPKLGQADP
                     ETRGFILSGGPMSSYEQGAPGLPAALLGSHLPILGICYGMQALALALGGEVRRAGEHE
                     YGLAEIRVLKNNPLLPGSSQTVWMSHGDQVTRLPTGWEALASSVNCPIAAAGDSARRL
                     FGLQFHPEVRHTPLGTEILRRFVLEICACAATWTPESIIQQSIARIREQAGGKQVLSA
                     VSGGVDSSVATALTRAALGGNVHSVFVDTGLLRLGEREQVEQAFSSLPGEPLRIVDAS
                     ERFLSKLRGATDPEQKRRIIGEAFIREFEAFAREVGSPRFLVQGTIYPDVVESQGIGA
                     SQAERIKSHHNVGALPKDMKFALIEPLRLLFKDEVRAVGEALGLPRSLVWRQPFPGPG
                     LAVRCLGEITPERLDTLRRADAIFTSELEREGLLTAAENAQGLPSGSSQAFAVLLPVR
                     SVGVMGDQRSYHEVVALRAVTTTDFMTADWARLPYELLARAAIRIVNEVPRVNRVVYD
                     ITSKPPATIEWE"
     gene            26403..27377
                     /locus_tag="PKL60_01205"
     CDS             26403..27377
                     /locus_tag="PKL60_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94783.1"
                     /translation="MDYAEILKKAWNIIWKHKILWLFGLLAGSAIGSGQGLSYRFSSQ
                     DRIQQFWQNNRGFPSVFERVSEFYRSLPPFAWFLIAGAAVAAVLFISLILFFVREYGL
                     AGVIKGSALADEPGRRGLKFSEVHRALKPYYWRLVLLRLLVVCAGAILAGLLAMPTII
                     FIIGTLGIGLCCLIPFFILLIPVGWGASVLVHNACIALVDENLGVVEALSRAWDVTIQ
                     NIGPLLVLHLLLGVVRLVTGLLMSIPMLAAALPLILALRSPGSAWPIAAGLLTGLLWL
                     ILLPLGLAGMSILNAFDLSARALAFREVKDKARVLPAGSQELPRQETN"
     gene            27382..29280
                     /locus_tag="PKL60_01210"
     CDS             27382..29280
                     /locus_tag="PKL60_01210"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNW94784.1"
                     /translation="MSLPALSPPGAAYHAAEHRGGLLPRGFLRRCYCALVFFLLVLQP
                     ALPANASYPGQASPMLEILNIDTREFPLIRAEIKPDNLPVGLAVPINAGTLSLLENGN
                     TIPARDVQEKSQGIYFALTVNPDFALDSRDPKGISRYAKLVGAFKQMGSAFSAEGVDR
                     FSLFINPDYVFEQLSDFGSLVTALEGYSENFRTQKSDLTSLSRAIDALSMDESGKDKV
                     LLYITGLPTIQDAKSIQLLSEIAREKHIKVIIWLAGEAFIATYPQIPYLQDLADSSAG
                     SLFLYSGSEPLPEADTYLNNLGRTYLVSYLSLVRESGTQSLSARLQLESSTLSSTSSS
                     FEVQVEPAELSFLNLPQELTLLQSADGALTPAELPLEVLVDFVDGHPRNITSARLLVN
                     GADVQENIQAPFNSFVLSLKDYAGKDKLALQVLMVDDLGLEARTPSTEIALSTILPST
                     GGAGIFRSPWLLLTAGAALAALVALVLLPSLRKAKSKPLASLSAEAALPAPLPSPAIL
                     ATLTRLSPENLPTPEKPIAITQEITIIGRDADLCNLVLDDPAVEAMHCQLRKLPDGEF
                     RLTDFRSAAGTWVNYAPVGAKGIRLQHGDLIQLGSQTFRFGSGSRVAAVEKNQAHRDL
                     TDGSSPAE"
     gene            29769..29853
                     /locus_tag="PKL60_01215"
     tRNA            29769..29853
                     /locus_tag="PKL60_01215"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29803..29805,aa:Ser,seq:tga)
     gene            29875..29963
                     /locus_tag="PKL60_01220"
     tRNA            29875..29963
                     /locus_tag="PKL60_01220"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29909..29911,aa:Ser,seq:gct)
     gene            30474..32276
                     /locus_tag="PKL60_01225"
     CDS             30474..32276
                     /locus_tag="PKL60_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94785.1"
                     /translation="MKKAKVFLIIIVISLFSVSLLGLKGPQIAKAEDENPPDSELIQQ
                     NAWLLGTEPAQSASSATVFSLSGCNQEFWDNGEFITHPGGGYQGANVSALQTALGMQT
                     YGFTNQYFSTGYHTADDFIITDTRGWQLDNISFFAYQDGDYPFPPSSPLVEVYLQIWD
                     GPPNDPNSTVVFGDLSTNRLISTSWTKTYRAADNSLMDDSMPIMENVVYVGAFLPAGT
                     YWLDWATKGSMSTGPWIPPVTELGQTTTGNAWVFKDSWQPALDAGTGTGQDLPFIIRG
                     CREQGPWQQLSNSQVDRMDNVLAAYDGKIWDVTGRGETGVSSYDPATGLWSLVSASTP
                     PFGSNYARSGCQVGNEVFIYGDGVTAGFTGLWSYNLSTNLWKQETPSGNPPAYQGIWA
                     PAWAADPATKICYMTGGAHTPGGGTLTSVYVYDTMQDKWLTPLRAFDSVRDYHAAFLF
                     TRQSDSHKLLCVVGGVTTGPVLTTTQCYDFSKANWNEEDIDLGPLPIALWGMGYAQTT
                     ISGEVQLWLVNGADGGFNISPETWIFSVKTKTWASAGSLQSGDFLRTSAVTMDNVVYH
                     VGGSLGGFNYAGKADKYIGFRSYLPCASKFSQLK"
     gene            complement(32536..33708)
                     /locus_tag="PKL60_01230"
     CDS             complement(32536..33708)
                     /locus_tag="PKL60_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HNW94786.1"
                     /translation="MSEFINNASKRKDALKAIIRKLHAGESVESLQEEFNEVIAEASA
                     SDVAQAEREVIAEGLPVADIQKLCDLHVAVFQQGLENQTAPESVPGHPVYNFRLENEV
                     ISRVLESIEGSLARVLAGDKNALKSLRQAAGNLNLIERHYEWKENLLFPVLEKYGFEG
                     PSKVMWGVHNDIRKAIRNLQSLMNSDPLDLETVDAEYLDLAKAVRDMFYKEEKVLLPE
                     SLSRLTQADWDMIKAESLSLKKDAEPAREEPAPEPAAEGKIKLSTGALTQEQINLMLT
                     KLPVDITFVDENDKVCFFSQTPERVFVRTAAIIGRSVQNCHPPQSVHVVQHILDDFRA
                     GKRDAAEFWIQMGGKFVYIRYFALRDEQGTYKGVIEVTQDVSAMRSLQGERRLLDE"
     gene            complement(33724..33948)
                     /locus_tag="PKL60_01235"
     CDS             complement(33724..33948)
                     /locus_tag="PKL60_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNW94787.1"
                     /translation="MTNMHALPVIDLALCSRCGECAAACPEHALAMDAENSTPVFAEA
                     DACTYCTECEQVCPQHAIQCPLTISWGTEE"
     gene            complement(34045..34728)
                     /locus_tag="PKL60_01240"
     CDS             complement(34045..34728)
                     /locus_tag="PKL60_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNW94788.1"
                     /translation="MTGAEQDLARRLGQFELFSMLPADLLLRMAKSSVLHSYGAREEV
                     FCEGDEINSLLLVLEGDVCAVKRSSDGREQVLAHLKAGDLISLAPLLLQKNACHTESG
                     RASHPTKVLSISLELFHEIAESSPEFHRALQRCLAGRLEQLSFLAASLSLQSARSRLA
                     AFLIRQADQGGQPAGITQEEIAAEIGTVREIVARLLRDFARAGWLERKRQHLVLKNRA
                     ALEEEAQRW"
     gene            34964..35674
                     /gene="fadR"
                     /locus_tag="PKL60_01245"
     CDS             34964..35674
                     /gene="fadR"
                     /locus_tag="PKL60_01245"
                     /inference="COORDINATES: protein motif:HMM:NF003444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HNW94789.1"
                     /translation="MPEWSPPQKAAEITEIRLQDAILDGTFPINSHLPPERVLAEMLG
                     VTRPTLREALQRLSRDGWIEIRHGKNTRVKNYMQEGNLLILNYLAKNPQAQPPSFVPD
                     LLDVRATLAPVFTRQALLRNPEAVCEHLDQMQEIPEDAREFSAADLELQRVLTILSGN
                     PIYTLILNGFAELYRVMGPFYFSSPAARERSRRFYFELREAAEKRDAEAGERLTREMM
                     TESIAIWKDARKQGEQAK"
     gene            35671..37260
                     /locus_tag="PKL60_01250"
     CDS             35671..37260
                     /locus_tag="PKL60_01250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNW94790.1"
                     /translation="MNANKSRAETWQSLQQDWDIIVIGGGITGAGVFRLAAAQGLRVL
                     LLEQNDYASGTSSRSSKLIHGGFRYLRNRQFDITRESVREREVLLREAPGLVRPLGFI
                     LPYTDSGTQRAEFHLGVTLYDLMAPKWKHRGLSQTDVHSLVPQLSAPWLAGAYLYYDA
                     VMDDARMVLRLLSEGCRAGGTALNYARVEKLLTTREGTVCGAAVSDQSPQALGSLELH
                     ARVLINAAGPWSDMLRAQVNAPTRLRPQRGSHLVFPYEKLPLPHAVTLLHPKDKRAMF
                     AIPWEGTTIIGTTDLDHPSPLDEGEPFCTQAEVDYILEAAAAFFPSARLSQADALSSF
                     AGLRPIIRGNAANPSAESRAHVVWEEKGLVTITGGKLTIFRRMAQDALRKAAPRLSLT
                     LKSHARFYDALPRQSGADSTELNPGQRCVLAGKYGFALQELLSCAKPGELRPVQGLPT
                     IRAELRYTARRESVQHLDDLLLRRTRLGLLLPEGARAELPFLREITREELDWDDMRWE
                     AEVQRYTQIYERFYNSAPAGI"
     gene            37279..39021
                     /locus_tag="PKL60_01255"
     CDS             37279..39021
                     /locus_tag="PKL60_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNW94791.1"
                     /translation="MAKKEVFTPNWYEDVTPVGTYRSLLKWGDPAGIKHPNSGLVRLI
                     MDKFQLTEADLRAPLDLSLEQVEDSAPCRLPEEHLEFFRALCGDENVCTDTYTRIARS
                     YGKGMLDALRLRRKIIQNLPEIVVAPRSREEIARIVAYADEHRIPVTVFGGGSTVTRG
                     METTRGGITVDMSKHMNRVLAFNETNQTITVEAGMSGPKLEALLNNAVHEFGAARAYT
                     CGQFPQSFEYSSVGGWAVTRGAGQNSTYYGKSEDLVISQDYVTPIGDFRTCPQPRAAT
                     GPDFDQIMLGSEGAFGILVSVTFRIFRYMPENRKRFSYMFRTWEDAQAACREIMQAEI
                     GYPSVFRLSDTEETDVAMKLYHIEGTAADTILQKLGYKPMQKCLLLGFTDGEKGFCAN
                     LDRKIRRICRQHGAFELSLFGVTKSWEKDRFRDPYLREDLQDFGVLIDTLECSVTWEQ
                     MPRVHQEVRAFVKSRPQTVCMTHLSHAYPQGANLYFIFIARIGTIKEYLQLQYGILEA
                     IAKSGAAISHHHGVGKQTSPWLEETIGKPQMDVIRLLKKHFDPHNIMNPGGTLGLDMS
                     AEQREKRWSMDLEG"
     gene            39021..40583
                     /locus_tag="PKL60_01260"
     CDS             39021..40583
                     /locus_tag="PKL60_01260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNW94792.1"
                     /translation="MSSEQLLAIDNGTQSVRALIFDLRGNLLAKSQVPIEAYYSAEPG
                     WAEQDPQVFWNALCQACQALWAIPGVEKSALAGVALTTQRSTIINVDAHGQPLRPAII
                     WLDQRRTPGLKPVGGIWGLLFRLAGMRGTVAYLQSEAEANWLRTYQKDIWEKTHKYLL
                     LSGYLTYRLTGAYIDSIGSQVGYLPFDYKNLRWSAKSDWKWEAVPVRPETLPELVRPT
                     ELLGQISAEASAATGIPEGLPLIAAAADKACEVIGAGCIEPSTACLSFGTTATVNTTH
                     KDYLEVIPLIPPYPAAIPGMYSLEIQIFRGYWMVSWFKREFGMNEERLAQERGVKTED
                     VFDELINSVPAGSLGLMLQPYWSPGLKQPGPEAKGAVIGFGDVHTRPYFYRSIIEGLS
                     YALREGADRTNRRSRQKIESIRVAGGGSASSAAVQLTADIFGLPASRPHVIEASGLGA
                     AMDLAVGLKLHPDFETAVREMTRISETFEPNPENHKLYDELYQRVYLKMYKRLRPLYE
                     ELADITGYPALD"
BASE COUNT         8715 a        12450 c        11652 g         8378 t
ORIGIN      
        1 catcggactt ttgggacact ccaaacgttt ttttcttccc cagcggctgg accaagccaa
       61 aatggagccg cgcgccgcgg gggaaagggt cgaggttagg atatgaaaca ttagagaaaa
      121 gaaaaaataa ggggcatatc tgcgaggaaa acccgccctg atgacttcat ccacgaatta
      181 ggagattccg attcagcctt gacaggagca tgtttccata aaagaacaaa acaatgacaa
      241 gccccaggtg ggatactgcc acccgcctat tctgcccatg cccaaaattc acgcgccggc
      301 ctgacttttc attcagtaga atcgagcttg tggggagata cgtctgaata cgctctccga
      361 cagcctgata tgcacatgtt gtcctgcacc aaaatgcttc gaacccccaa attaccccct
      421 tccaaccgca aaaatcacga tttcgagaag gaaatcaatc ctttctcttc tttttccggg
      481 taaccagcaa agcagcaccc acaaagcctg ccattgtcgc aatcccgagc cagcggctct
      541 tctgcccgcc tgttggacct ctccgaacgg cagcatttgg tatttcctgt ccgctgcgcg
      601 cctgcccgtt ccaatccagc gtcaccaggc ggtccaaaaa gccctgattc gcgcaggatt
      661 caagcgtaat cacatacacg gcactgctct gctggcgggc ggcgcagagc tcccgggcga
      721 attcctgcca ggtcttgggc tcttcatccg tggaagtgcc caggttcaag cttttgcgtg
      781 cattgaccac gcgtaccgct cccttgccat cggcgccgcc ctcacgaaaa gcgcgcgagg
      841 tttcgtttat attaggcggc aggtggatac agcgttcagc tttaggtact tccggcatcg
      901 ttttcctgcc caagcccaaa ttctttattt ggcttggggc gccttgcggc agcgaatata
      961 ccagctccac cgcaaatcca tccgcgtgcg cgcgctcaat taatgtggat atggcagccg
     1021 aagccgtgag cccattggtg cttttctgtg cggctctctc ctctctctca ggcacgccat
     1081 agccgattgc ggaccagcgc agccctttca ccccagacca atgaatcagg gcttgataca
     1141 gctccgcggc ctgcgccgag tcagctctgt ccagccaggt gccttgttcg cccggcagca
     1201 gcagccaggc ggaaaccgga atgccaagtt tattcagcag gcgcgccgct tgggccctct
     1261 cttcagaaaa atcgcgcaca accagcgcca cgcccgcccc caggctcttc aggtcgtcca
     1321 gcacaaaacg gtcctcgaac agcgcacgca acgctctggg ttccagagcg caaagaaacg
     1381 tcaaatttgg gagtgtgttc agcgatgttt gcatcttaag aaattaaccc cgcccgctgt
     1441 cttctcgttg cgcgggtgag ccgggaactt tatcccagcg gctgtttggc aggcaggcct
     1501 gttcgccggg ggaactccgc tgaagttcgc gcgaccttct ccagcaccac cagatagcgt
     1561 tcgtccgcga cgcccggcag caccacgggc aggattacgc ggattttgcc gcccagccgt
     1621 tgaatcactt tttcgctttt cagcgcttcc aactgtgcgc tctcgccttt ctgcataatc
     1681 accagcccgc ctatacgcac caggggcagc aggtactcca ccagcgtggg cattgctgct
     1741 accgcccggg cagtcgccag gtcgtaactc tcgcggtgat tgggatcctg cccgacttcc
     1801 tcggcgcgtt ccgccagaac ctgcacctgg cgtaagccaa gcttctccac gatatgcgcg
     1861 caaaagttgg ccttcttctg gacggactca accagggtca gccgcagctg cggcaggatt
     1921 aacttgaggg gaatcccggg gaaacccgcg ccggtaccaa tgtcaatcag actgcgcgga
     1981 gacgcggacg catccagtgc catcaggcag gtcagcgaat ccaaaaaatg cttggtctca
     2041 atcccttctt tatcgcgaat ggcggtaagg ttgaagcggc tgttccactc caaaagctca
     2101 tcttccagga gcttgaatgc aagtatttgc tcgcgggtga gttggaggcc caggagctgg
     2161 cgcgcgtttt gggtgaaggt ttccacactc gggattataa cttaaagggg agcgctgcag
     2221 cgtctcgcgg cgtgttccaa caggcatatt caggcaaaga gtgaaaacag acccgcccca
     2281 aggtaagcaa acaacagcat tttcgcaatt ttcccgaccg cgcagaacag caggaatttc
     2341 caggctggga cttttaacga accggcaata aacccggcga tatcaaacaa gggattggga
     2401 ataaaggcca gcacgaggat cgtccacagc ccgtatttcc gcatccaccc gagcaccttc
     2461 tgataattgt ccttgttttc gaccagaagc tgcccgctga cccccgccag gtatcctgaa
     2521 agctcgccaa cagcagcccc caagccggcg gccagcgcca cccaagcggg gtggaagacc
     2581 gtgcccatcg ctgaggtgaa cacgacccca ggcaaaggaa acaggatggt tgcattggaa
     2641 agcacggaaa aaagaaaaat gcccgggtat ccgaaagcct gcagcttctg tacctgggtt
     2701 ctgaagatga ttatcagcgc ggtaaccgcg attaacacaa gcagcagcag cacccgccag
     2761 aggacttgag tccgcgtgaa gcctgtttta cccggctgac tggcttgcgt cattcagcag
     2821 accctgcagg cgcgcgataa ccatatccaa ggcaacggta ttctttcccc cttcaggaat
     2881 aataacatcc gcaaaacgct tggagggctc tacgaaatcg atgtaactgg gacgaacagt
     2941 cgtcaggtat tgattcacga ccgattctac agtgcggccg cgttcttcga tatcgcgcgt
     3001 gagccggcgg atgaagcaaa tgtcggagtc agtttccacg aatactttca tgtcaaaaat
     3061 cttgcgaagc ttcgcttccc aaaaaatcag gatgccctcc accatgatga tctgacacgg
     3121 ttctatcatc acgacttccg cggagcgcgt gtgctgttta tagtcgtaga ccggccttct
     3181 gaccgccttg ccggctttca gcgcgacaat atgcctgatt aacaggtctg tttccagcga
     3241 gtcggggtgg tcgtaattaa tcttcagacg ttctgcgtag ctgagctcgg cctggtcgcg
     3301 gtagtaagcg tcatgcggta aaaaggcgat tttatcgcgt ccgatcgcgt ccaggattga
     3361 gaaggcaagc gttgttttcc cggagccgga cgcgccggct atgcctatca ccagaggaga
     3421 tctgccagtg tccataaatt aattatatcc tcgattcggg tataatttca tcaccaactg
     3481 catagaggaa acatgacagc tcacctgatc gatggtaaac aaatcgccgc agagatacgt
     3541 ggggatgtga aacttaaagt ggacgagcgc atccagcgcg gactgcccat accaggcctg
     3601 gcaacggtgt tggcagggga aaaccccgct tcgcaggtct atgtgcgcaa caaacaaaag
     3661 agctgcgctg aagtcggcat ccgctccttc gggcacacgc tgcccgctga tgccagccag
     3721 gaagaggtcg aagcgctggt tcacaagctg aacgctgacc ccgaagtgaa cggcatcttg
     3781 gtgcagctgc ccctgcccgc ccacctgaac gaagaacgcg ttctcagcgc gattgacctg
     3841 agcaaagacg tagacggttt ccacccactc aatatcggca ggctggcgca aaaaggacgc
     3901 gaacccttgt ttattccctg caccccctcg ggcgtgatgg tgctgctcga gaaagcaggc
     3961 gcgcggctgt ccggagcgaa cgcggtcgtg cttgggcgtt ccaatatcgt cggcatgcct
     4021 gtggcgctcc tgcttgtgcg ggccgacgct acggtaacca tctgccactc caagacgcgc
     4081 gacctgccca cgctcgtccg ccaggcggat gtgcttgtcg cggcaattgg caggcctgag
     4141 atggtacgcg gggactggct taaacccggc gcgattgtga ttgatgtagg catcaaccgg
     4201 gtggaagatg cctccgcgcc caaaggctcg cgcttggtcg gcgacgtggc tttcgaagaa
     4261 gctttggatg tcgcctcggc aatcactccg gttcccggcg gcgtagggcc catgaccatc
     4321 gccatgctcc tacagaacac actgcgcgcc cgcgaactgg cggacggcgc cgcttaaccc
     4381 gcctttgcca aaggagaaga ggatgccgac gctgctggtt aaaaatgccg ctctcatcgt
     4441 aacgatggac gcgcaggagc gcgaactgca ggatgcggct gtcttctgcc gggacgggtt
     4501 catcgaacaa atcggcgcgg ctgctgacct cccttccgag gcagatgagg tgctggacct
     4561 ggccgggcat atcgtcctgc ctgggctggt caatacccac catcactttt accagacgct
     4621 cacgcgcgcg ttaccagctg cgcaggacgc caacctgttc aactggctca aaacgcttta
     4681 cccgatttgg gcgcgcatgg accccgacgc ggtctttctc agtacgcaaa ccgcgctggc
     4741 ggagctggcg ctttccggct gcaccactac ttcggaccac ctgtacctct tccccaacgg
     4801 ttcgcgcctc gatgatgaaa ttgacgccgc tgccgaaatc ggcctgcgct tgcacgcttc
     4861 gcgcggctcg atgagcctgg gtgaatccaa aggtgggctg ccgcccgatt cagtcgtcga
     4921 cagcgaagat gccatcctgc gggacagcca gcgcctgatt gagcgctacc acgacccaaa
     4981 gccgggctcc cggctgcaaa tcgtgctggc gccctgctcc ccattcagcg tcacgggcga
     5041 actgatgcgt gaatcggcca ggctggcccg ggagtacggc gtacatctgc acacgcacct
     5101 ggccgagacc gaagacgaag acgacttttg cctgcaaaag ttcgggtata aaccggtcgc
     5161 gtatatgcag accgtggact ggattggcag cgatgtctgg tttgcccaca gtgtgcacgt
     5221 gaacgccgcc gaaatcgacc tctacgcccg gcacggctgc ggcgtggcgc actgtccgag
     5281 ctccaacatg cgcctggctt cgggtatcgc tccggtgatg cagatgcttt cccgcggagt
     5341 caagctcggt ttgggcgtgg acggctcggc cagcaatgac agttcgcaca tgctggcgga
     5401 agcccgccag gcgatgctgc tttcgcgtct gaacagcggc gtgcacggcg ccagccgctc
     5461 cgcgccggac gcgccgcccc tgatgaccgc ccgtcaggcg ctctccattg ccacgcgcgg
     5521 cggcgctgcc gtactggggc ggcaggatat cggttcgctg gagccaggta aatgcgcgga
     5581 cttcttcgcg atcaatctta accggctgga gtacaccggc gcctggcagg acccgctggc
     5641 ggcggtcgtt ttctgctcgc cggtccaagc ggattggaca gtagtcggcg ggcgcccggt
     5701 cgtgaaagag ggccggctgc tcagcctcga cctgccgcgc catattgaaa agcacaacgc
     5761 agcttccagg aagatgatcc tgggctgaca catattcata ttattcaaag gatccctatg
     5821 gagaaaaaag gtttggcagt tgttgcggtc ggtggaaacg ccctgattaa ggataaaagc
     5881 catcagacgg ttccggatca gtatcaagcc gccgcggata ccatggtcca catcgcgacc
     5941 atgatcgaag agggttggga tgtggtcgtc acccacggaa acggaccgca ggtcggtttt
     6001 atcttgcgcc gttcggaaat cgccgcgcat gagttgcacg aggtcccgct ggactactgc
     6061 ggcgcggata cgcaaggcgc gatcgggtac atgttccagc aagctcttta caacgaattc
     6121 ctcaagcgcg gcattcgcaa gagcgccgcg acggtcgtga cccagaccgt cgtggacagc
     6181 gaagacccgg ccttcaaaaa cccgaccaaa cccatcggtt ccttcctgga tgaagcccag
     6241 gcaaaagtta agatggaaaa agacggatgg acaatggtgg aagacgccgg ccgcggctgg
     6301 cgccgcgtgg ttccctctcc catcccgcag gtcattgtag aagccgacgc gattcgcagc
     6361 ctgattgatc agggcttcgt ggtcatcgcg gttggcggcg ggggcatccc ggtcatacgc
     6421 acgcccgaag gcaaacttgt cggcgtggaa gccgtgatcg acaaagactt cggttccgcc
     6481 attctagcct gcatgattaa ggcggattta ttcctgatta gcacggcggt agaaaaagtc
     6541 gccatcaatt tcaacaaacc cgaccagaaa tggctggacc gcatcactgc cgctgaagcg
     6601 cgccaatatc tcaaggaagg tcactttgcc aaaggcagca tgctgcccaa aatcgaagca
     6661 atcctcaagt acctggattg cggcgggaaa gctgccctga taacagatcc cgaacacatc
     6721 aaagacgcgc tggacggcaa aaccggcacc tggattttgc cctgaggtct ggcaccttta
     6781 ggggaagaga gccgagagtt tttcatgagg atttcgcatt acacctgttc tgtttgcggg
     6841 gctgagtttt cggagacgga aacccagtac gtctgcccgc gcgacggcgg caacctgacc
     6901 gtccacctgg acttcgaaac cgcagccgtg agacccaacc ccaacgacat tctggaaagc
     6961 cgggaggcgt ccatctggcg ctacctcccg ctgctgccgg tcgaggaccc gggctatcac
     7021 aacacgccgc tgcgccaggt tggttggacc ccggtgtatc gcccccaaac gctgcagcag
     7081 gaactggggt tacaccgctt gtggattaaa gacgagagcc gcaaccccag cgcctccctt
     7141 aaggaccgcg ccagcgccct ggtgacagcc cgcatgaaga tgaccaacca ggaagttgtc
     7201 gtggctgcct ccaccggcaa cgccggcgca gccacagcct gcatggccgc cagcgctggc
     7261 ttgcgtgcca tcattttcgc cccgcgcacg gcgcctcagg ccaaggtagc ccagctgctc
     7321 agctacggcg cgcgtgtcct cctggtggac ggcagctatg accaggcttt tgacctgagc
     7381 ctggctgcct ccgatacctt tggttggtac aaccgcaaca ccggccataa ccccttcacc
     7441 gcggaaggca aaaagaccgc cgcgtttgaa atctgggaac aggtactgcg ccatctgccc
     7501 gccgaatccg aagcgctgcg cgtttacatc ccggttggcg acggaaatat catctcgggc
     7561 atccacaagg gcttccagga tttgcttgcg ctgggttgga tagagcgcct gcccgtgctg
     7621 atcggcgtcc aagcggagaa atcggcagca atcgccaacg cgttcttcac caaagctgat
     7681 gcgattactc cggtacaagc caccaccctg gcggacagta tcagcgtgga cctgccccgc
     7741 gatggtctgc gcgccctgca agctgtccgc gctacgcacg ggcattatgt gaccgtccct
     7801 gacgaagaaa ttctgatggc aattccggag ctgggaagag ccggcatctt tgccgaaccg
     7861 gctgccgcag ccgcctttgc cgggctgcgt aaggacctgc gcgaagggca gctgaacccg
     7921 gacagcccca cgctggtcct gtgtaccggc agtgggctga aggacgcacg cgcagcgata
     7981 agcgcagtgc cttccgcccc gattatcgaa ccgactatcc aggcactaat gaaggtgatc
     8041 catgtctgaa cctgtttttt ccatcatcgc cccgattttt aacgaactcg aaaacctgcc
     8101 cctgctctac gcccgcgtgc gcgaggagct ggagaaaacc gggcaggagt gggaactcat
     8161 catggttgat gacggcagca aggacggctc caccgagctt atccgcaagt tggcagccgc
     8221 ggatgaacgc gtccggccgg tgatcttcgc gcggaacttt ggccaccaaa tcgccgtcag
     8281 cgccggcctg gattacagcc gtggtgccgc ggtcatcgtc atcgattccg acctgcagga
     8341 cccgccggaa gtcatcggcg aactgattgc caaatggcgc gaagggtttg aggtggtcta
     8401 cgcggtgcgc accgaacggg aaggcgaaac caaattcaag acccaaaccg cctccctgtt
     8461 ctaccgcatg attgaccgca tcacggatat caaaatacca ctggataccg gcgattttcg
     8521 tctgatggac cgcaaggtgg tcaatattat ggtggcaatg cgcgagcaca accgtttttt
     8581 gcgcggcatg agcgcctgga ccggcttcag gcaggtcggc gtggagtaca aacgcaaagc
     8641 ccgccatgcc ggcgtaacca agtacccgct caagaaaatg ctgcgcctgg cgatgaacgc
     8701 cgtgactggc ttttccatgt ggcctttgca gcttgccacc cgcctgggcc tcatcctggg
     8761 cgcgctgggc gtattggcca tcccggtggt catcgtgctg cgctatgcag cggatgtaca
     8821 gttccacggc ctggcgacgc tcgccatcct aatgacgctc ctgagcgcgt taatcctggt
     8881 ctttctgggg ttcattggcg aatatcttgg cagagtttat gacgaagcca agaaccgccc
     8941 cctctacatc gtcgctgaag gaccgcggga gccttttaat cccgtacgac tggacctgcc
     9001 agccgaaacc aaaagtaatc attcctgaac ctggaggaaa ccatgacaaa aatcgacctt
     9061 accctaatgc ccgaaaacct gcagcgcaca acccaacgcg tgcgtgagaa acgattctac
     9121 attcccacgc tcgcccaaca aaaagacccc gcgctcatcc cggcgcatat taaggaacag
     9181 ctcaagaccg ttggactgtg ggacctggac ccgctgaatc tgttccgcat cacgtggcat
     9241 aacgaagcgc gcgcttctgg ggggctgttt ggtaacgtca actatgtgga attccccccg
     9301 gaactgacgg gcgtgcctgc gcgcgttgtg gcgctggttg gcaagtggtt cccgaccgga
     9361 tgccataagg ttggcgcggc gtttggctgt ctggttccgc gtctggtgac cggtcaattc
     9421 gaccccaccc gccaaaaggc ggtctggcct tccaccggca actactgccg gggcggcgca
     9481 tacgattcct atttgctggg ctgtgaatcc atcgccatcc tcccggaagg catgagccgt
     9541 gaacgctttg aatggctcag caggattgcc agcgaggtca ttcccacgcc gggcagtgag
     9601 tcgaatgtca aggaaatttt cgacaaatgt tgggagctgc ggcgcagtgg gcaggatttg
     9661 gtcattttta accaattcga agaattcggc aactacctgt ggcattacac catcaccgga
     9721 agcgcggtgg aagaggtcgt tgcgcagctc agccgcccgg gcgacgccta ccgcggattc
     9781 gtttccgcga cgggttccgc tggaaccatt gctgccggag actacctcaa gcagcgcttc
     9841 cccaccagca aaattgtggc cagtgaagcc cagcaatgcc cgaccttgct caccaacggt
     9901 tttggcgcgc accgtattga gggcattggc gacaaacatg ttccctggat ccacaacgtg
     9961 cgcaacaccg atttcgtcac cgccatcgac gaccaggccg tggtaaatct cgcgcgcctg
    10021 ttcaatgaac ccgccgggcg cgagcatctg attaactccg gcgtggatga aacgctcgtg
    10081 aatcaactgg acctgctcgg cttttctggc atttccaacc tggtctccgc aatcaagttc
    10141 gccaaataca acgaactgac cagccgcgat attgtcgtaa ccgtcttgac cgactcgatg
    10201 gagctatacc gcagccgcct gaccgaaatg caggagcaat atgggcagta cagcccggcg
    10261 gacgcgattg cggatgacgc caggtatctc aaaggtcagc cgattgactt catgcaagag
    10321 ctgagctact acgaccgcaa acgcatccac aacctgaagt attacacctg ggtggagcag
    10381 cagggcagaa cctacgaaga aatccaggcg cagtggtacg acccggatta ttggctggaa
    10441 attcaggcgc aggcgcctga gattgacgcg cggattgaag aatttaaccg tgcggccgcg
    10501 gcataaatcc agacgatgaa tccgaaaagc acagctccgc ccgatacagc cctggtactc
    10561 gtcgccatcg cgcgcgacca acgcgacctg gaaatcgccc gggtgttggg ttggtatcgc
    10621 atcccgctga agagcgcgcc aaaagtgatt tccgtggatt atcttgcgtt ttaccagacc
    10681 agcgctttcc cgacggcgga acgctggcaa atccgctgga tagctccgat tttggggcac
    10741 gaactgacca cccgcgccga actgctgcgc gaccaaccgg accacccgcg cgcgcctgag
    10801 gaatacttta agctccagct gggaccgctg caggaacttg cgcagccaat cccggcagac
    10861 acctggaagc gcgtaacctt cttctacacc accttcggac gcgtgcggca ggccgctgtg
    10921 ttggcggatt tgccagtcca cgacgaagaa cgcgccgtcc tatggcagtc cctgcgcgaa
    10981 cgcgcgctga gagcaaacga atatcaggcc gcggagctgc cgcaaatgcc ggttgacccg
    11041 gcgatcctgg cgctcttcag cggcgggttc agacctaccc acccggaata agcgcttcag
    11101 gttcagatag gctcaatgca aaagatttcc ccttatcgga gcggaaaaat gcgaaaactc
    11161 atcaaaaatg ggaccgtcgt caacgccgaa agcagccagc aggctgatat cctggtcgaa
    11221 ggcgaaaaga ttacccgcgt ggaacccggg cttgactgcc cggacgcgga agtcattgac
    11281 gccgcaggta tgctcatcct tccgggcggg attgacccgc atgtgcatat caacctgccc
    11341 atggcgggaa cgacctccag cgacgattac tacaccgccg gtaaggcggc cgcctttggc
    11401 ggcaccacca gcttgattga ctttgtgtcg caggacgagg gcagcctggc ggagaatatc
    11461 gcccgcaagc tgcaggaatc ggacgggcgc gccagcgtcg atttcgggct gcacatgaac
    11521 atcacccgct ttacccccgg ggtgctggac gaaattgcca gcctgccgga actgggcatc
    11581 accagcgtca aggtctttac cgcctacaac cagcgcttgc gcctgggcga cggggaaatc
    11641 ttccagacga tgcgcgccgc cgcgaaacac ggcctgctca cattggtgca cgccgaaaac
    11701 ggcgatgtca tcgatgtgct cgtcgcggaa gcgctgcagc aagggcatac ctccccggtt
    11761 tggcatgctt atacgcgccc tgcctggggc gaagtggaag ccagcctgcg ggctatggct
    11821 ttggcagccc aggcggacgc gccgctttac ctggtgcaca tgaacgtagc aggcgaggtg
    11881 gaccagctgg cttacggacg cgcccacggc gtccgcgcga tgggcgaaac ctgcccgcag
    11941 tatctgttct tcacagcgca agacctggaa cgcccggacg gcacgaagtg gttgtgctcc
    12001 ccaccgctgc gcagcccggc tgaccaacag ggcttatggc agggcatcga taacggcacc
    12061 atccaggtcc tcgccactga ccactgcccc ttcttctatg acggcacccg cgcaatttcc
    12121 tacgaagacc aacaagtggc attgccggga aaagagctcg gaaaagggat cttcacgcag
    12181 gtgccgaacg gcgtgcccgg cgtcggggac cgcctgccgg tattctggac gcacgctgtg
    12241 cattccggca gaatcagccc cagccaattc gcggcgctca cttcggcgaa cgccgcccgt
    12301 atcttcgggc tgtacccgcg caaaggttgc ctggctgccg gctcagacgc agatatcgcc
    12361 atctgggacc cggaaatgcg ggtgaattac ggcgcacaga ccgccaaaca ccgcacggat
    12421 tacaacctct acgaaggttg gcagctaacc ggctttccaa ggcaggtctt cctgcgcggg
    12481 gaatgcctgg tgcgcggtgg agaatggttc gggcgccctg gccagggccg attcctgcgc
    12541 cgccaaagcg gagaaatcat ttgaccagtt ctcaacgagg cagattgtgg taattcccct
    12601 gctcattctg ctcctgttag cggcttcagc cgccatcacg gtttcgcacg cgctcaaacc
    12661 gaaagtcgcg cgcgcctgga tgattgccct gattagcgcg ctggttgtct ggtttggttt
    12721 ctttatttta cggctgtatt tgccgttgag cttcgcgctc ccagcctgga aaccggaggc
    12781 gctctttatc tcagcgccca gcctggaact gaactatcag aactggcctt atgccgtggc
    12841 attggtcacg ctgtgcaccg cggttatcct gaccgactcg acccgcagct ttttaccttc
    12901 ctcccccaac gcgtgggcga atgccatcgc aatcaccgct gtcaatttga ttggcatatt
    12961 ggctggcgac ccgattacgc tgatgtttgc ctgggtgatc atcgatacca ttgagttcgt
    13021 gtacctctat cagacccggc tggaccgcag cagcgacgcg ggcttggctg gcttcatgac
    13081 catccgcgtc ctgagcgtga tggcgctggc cgccggcgcc gtaaccggct ggctctcgat
    13141 gccggccttt ggcatggcgg agattccaca gaaggccggc atctactttc taatggcggc
    13201 cgggctgcgc ctgggcgtac tgcccttgaa cctgcccgtg cagaacagca gcgaacccaa
    13261 acacggtccg gcgctgctca cacgcctggc gcctgtcgcg tccagcctgg cattgattgc
    13321 ccatctccct cagggcttgc taacttccca aagcgctttg cgcccgcttt ttcttgcgct
    13381 caccgccgcg gccgcgctgt acgcctcggt catgtggttg agcggaaaga accaacacag
    13441 cgccttaccc tactggatta ttacactggc cgccttcgcc atcacctgcg cgctgaacga
    13501 ccgcgcggaa gccagccggg tatggggcgt ggcgctgctg ctgagcggcg gcgcgctttt
    13561 tctgttcgac ccgcccatcc gccgtattcg ttttctcccg ctgctcgggc tgattggatt
    13621 atctgccctg ccgtataccc tcgctgccgg gggttgggac gggctgctgg cggataaatt
    13681 cacattcagc acggggttaa ccctgctttc gcacgccctg ctgctgctcg gctacctgcg
    13741 ttataccttc gagattagcg ggacggtcac cggtctcgaa aaacacgccc gcctcactta
    13801 cccgctgggc ctaatcctga ttgttcagac catcgtcatc atcggtctgg ccggctggcc
    13861 gggggtgctc accgcgggca agtggtgggc cagccttatc agccttgcgc tcacaggttt
    13921 gggagcgggg ctttatctca aacggggcgc aaaactcccg cttgccagcg ccgctgcgag
    13981 cctgccgctc tatcgcttct ggactttcct gctgaaatcg ttccagcagt tcctcagcct
    14041 gcagtggctg tacaaagcca ttgcctggct gataggtcag tttgccgcgc tggccggtct
    14101 gttcaaccag gtcctggatg gagagggcgg aatcctttgg tcgctcgtgt ttctggctgt
    14161 cctcatcacg ctgttccttt cgggggtaaa tccatgaccg gcgcctcctg gatgtatatt
    14221 ccagccgtgc ttgccatcgc agccgcgggc atcaatctca cccggcgctt atgggttgtc
    14281 aacgctgccg ctttggggct tcaatacctg ggggtgttcg tgctgctgac tacggttcgt
    14341 ccagccagcc tggcgctggt aaagctgctg gttgggtgga tggttacgtt gacgctgttc
    14401 ctctcgctca tcaccgacgg aaaaatccgc agcgcaaagg ctttcttctc cttcagcgcc
    14461 ggggaaatct tcagactgtt ggccggggtc ttcgcggtat ttttcctggc tgcgctcctg
    14521 ccgcagctgc aagcctccgt tttcccacag acctccctgc cgctgctgct ggcggccttc
    14581 agcctgctgg ttttggggct cttccaggcc agcctcagcg cggaaccgtt ctatgtcatc
    14641 atcgggttgt tgacctttct ctcgggcgtg gaattgctgt atgccagcct tgaactttcc
    14701 atcctgctgg aagcgctctt tgcgggcgta aacctgggcc tggcgctggt gggagccttt
    14761 tttgtcatca aggacgcgga gattccggaa gcatgagcac tccgctgatt tgggtcgtct
    14821 tcccgcttgg gttcgcactc ctattgatca ttacgcgcaa aaagcccatg ctttcgggca
    14881 tccttgcttc cgttttctgc ctgctgctgg cgcttgcggc gctgctcttc ccgcgcgatc
    14941 tcagctttct cctgccggac cagcgcctgg aaattgccag tactttggag cttttcggca
    15001 gaagcctgca gattaacgga aaccagctca ccataatcgg gctgctgtac ggcatcgcct
    15061 tcttctggaa tctgggcagc ctgctctaca gacccagcct atggtttaac atcctctcgc
    15121 ttgggatcac cgccctgtgg gttgccacgc tttccgtcaa tcctttcctg tacgccgcgg
    15181 tgttcatcga gttgatcgcg ctgctcagtg tgccgctgct ttctccgcgc ggggtgcgca
    15241 ccgagcatgg ggtactgcgc tacctggtgt ttcagaccct ggccttgcct ctgattttgc
    15301 ttaccggctg gatgctggcg ggcatcggct cggccccttc cgccaatcct ctcatcgtgc
    15361 gttctgcaat gctggtgctg ttcggcttcg cgctgtggat tggagccttc cccttccact
    15421 cctggattcc gatgatcagc gccaaaagcc acccctgggt ggtcagtttg ctgctcactt
    15481 tgctgcaaag cgctctatcg gttttcttat tgcattttct ggaccaatat gcctggctgc
    15541 gcaacctgcc ggtcactttc gccagtttgc gctggattgg cgtgacgatg attgccattg
    15601 ccggtctctt ttcagcggtg cagacaaacc ttggcaaaaa cttcgggtat ctggtgcttt
    15661 acgagaccgg ctactcgctg ctggcaattg gcctggcgca aagcggcggg ttgaattatc
    15721 tggcgatgct ttttatcccg agagtgttaa gctacgcgtt actcagcctg gcgctgtccg
    15781 ctttgtggga gaaaggcaat gcggaggcgt tggcgtacaa agcgcaggaa ggcttgttca
    15841 aaaggcgtcc ggtcgtttcg gctggaatcc tggcggcttt cttcagcctg ctcggcctgc
    15901 cgcttttgcc cgttttcccg cataaacaaa tgctgtggct gctgacagcc caaagcgacc
    15961 cgcgcctgct tgtatgggtt ctggtcggca gcctgggttt gctgctgagc atagcccgcc
    16021 tgctacgcct cttcgctcgc gaagggagcg ccgccgatga agccgggcca acgcgcgaga
    16081 agggcgggct aatcgcgctg atgctgctta tcctgctgct gatgctggcg atgggaataa
    16141 ccacgcagct gcttctgccg cgctttctgg acatcctctc acccttcacg cagctcgcgc
    16201 ccggcctata gaaaatcgtt cccggaaatg tgtataatca ggggaaagaa cccaggaaga
    16261 gcaggtaaaa aatggacgac atcacaagtt ttgaacaact tgaaaaaaga gtgcagtggc
    16321 tggataacga acgccgcaat gacaaaacca atcttgcctc cctacagaac cgcctgaccg
    16381 ggctggaaag cgaaaatatc agcctgcaca agcaaattaa agatttggag ctggagctca
    16441 gcggcctgaa aaatcagatt tccagcctgg ataagtacga gaaccgcatc gaacgcttga
    16501 acatcgatct gaccaaacaa gtgcgggatg tgaacgaacg cgctgaaatg aaccttaacg
    16561 aagcagtcaa acgactgaag ctggaaatcg acgcgaccaa gcgctcgctg agcgaaatct
    16621 accagattgg agaacagctt gagccaatcc gcaatgaact gaagtctcac aaagtggaag
    16681 atgcccgcct ggcgcggtta attgaagaac tcaaagggaa aatccaggag gtcagccgct
    16741 ttgatgagga ctaccgccgc tcgctgcact tgctggagga aaactaccgc caggaaaaca
    16801 agcgcctgaa cgatttccag ggcgatctgg ctgcccaccg caagcgcgtg gaggacaccc
    16861 gcagccgctt tgacgtgttc agcgacagtt tccgtacgct ggatgtgcgc atcaccgaat
    16921 tgcaaacctt tgagaaagac cgcaaggagg cgcagtcggt attcatcgaa aaggtaaaca
    16981 actccctgct ggataaggaa aagaccttcc gtgtctggga aaaacgcttc ggggaaattg
    17041 atcaagtgaa gctggacctg aatacccagt tggctgacct ggaaaacgcc cgaaaggccc
    17101 tggataaatc catcagcagt tcggaggagg tcgtcgcacg ctttgaacgg cgcatcaacg
    17161 aaatcagtga aatccaacgc ctgaacgatg aacgcttccg ccaggaatgg aactccttca
    17221 aagctgatga cctgaaacgc tggacaaatt acttgcttgg gcaggaagaa cagctgcgca
    17281 ccatcaacca gcagctcagt tccgcgcatg aggagttgga agcgctcaag gattcctccg
    17341 tgaagatcca ggatgagctg gataagctta cccgcgagac tttccgcagc attcagagca
    17401 tcctcggggc atatcaggaa tcaattcaga gcctggctcc gctcgcggag aagaaagccc
    17461 agtaattccg tgcacatcgt aggcaccgcc gggcacgttg accacggcaa atcgaccctg
    17521 gtcaccgctc tgaccggcat aaaccctgac cgcctgaagg aagagcgcga gcgggagatg
    17581 acgattgacc tgggctttgc ctcgctgacg ctccccaacg gggagaaggt gggcatcatt
    17641 gatgtgcccg ggcaccgcga ttttatcggc aatatgctgg cgggcattgg cggtattgat
    17701 gccgtgctgc tcatcattgc tgccgatgaa ggcgtttccg cccaaacccg cgagcacctt
    17761 gccatcctcg acctgctcca catcacctgc ggcttggtcg tgctgaccaa gctggacctg
    17821 gtctccgacc cggattggct ggagttagtc gagctcgagg cacgcgagac cctgaaaggc
    17881 accagtctgg aagacgcgcc cgtgctgagg gtttccgcgc gcaccggcgc gggcattcct
    17941 gaactgctgg agaagctgca ggaagtgctg gaccacacgc cctccaaacg cgacctgggc
    18001 aggccgcgtc tgcccgtcga ccgcgttttc acattaaccg gctttggaac agtcgtcacc
    18061 ggcaccctgc tggacggcag tctggccgtc ggtgatgaag tcgtaagcct gccggaaggc
    18121 ctgagcgcgc gcgtgcgcgg gctgcaaaac cacaaccaga agctgcagaa gctcgccccc
    18181 ggcgcgcgcg cggcggtcaa cctggttgga attgaaaaga accagattgc ccgcggcagc
    18241 gtcatcacca aacctggcgc gtatacccca acgaccctgc ttgatattca tttccgctat
    18301 ctggctgatt cttctttcga gctgcggcac aacactcagg tcaaaatcta catcggagct
    18361 gccgaagccg ccggtcagct gcgcctgctg ggcaaagagc agctaaaacc cggcgaacaa
    18421 gcttttctgc aaattcgtct tgccagcccg gtggtggcag cgcgcggcga ccggctgatt
    18481 atccgcctgc cctcgccgcc ggaaacactg ggcggcggcg tggtgctcga cccgcacccc
    18541 gcgcgcctgc ataaacgttt cagcgagcct gtgctgcgaa acctggaagg gctgctctcg
    18601 ggcggcgaat ccgaagcgct cctgcaggcg ctcaaagcgc tgcgcgcggc cagttcggag
    18661 gaagtgttag ccagggccgg catggacagg caagcgagcc tggcgctgct ggacggctta
    18721 attagcagcg gaagcatcat accgctccaa actgtcaagg agccttcacg aaacctcctc
    18781 atgaaagctg aaacctggca gcaagtgcgc gcggagctgc tcggagcgct cgcggagttc
    18841 cacgcgggca gccctctaaa acccggcata gcgcgcgaac tcttgcgcgg cgcctgtaaa
    18901 cttccaaccc agctttttga cgctgcgctg gaaaaattgc gcgaggacca ggaagtgaca
    18961 gttcagggcg cgctggccgc gttgtccagc caccggatag cctacagccc ggaccagcaa
    19021 gcgcaaatat cgatgctgct gcggcgcttt gaggagagcc cttactcccc gccggatgcg
    19081 cccgaagcag ccaggctgat aggcgcgccg ctattggagg ccctggtagc caccggaacg
    19141 ctggtccagg tttccccgca ggtactattt tctccgcagg ctctcgcgga aatgagcgcc
    19201 tgggtggagg ccagcataaa agagcagggc agcctcacgc tggcagaatt ccgcgaccat
    19261 ttcagcacca gccgcaaata cgcggccgcg ctgctggaat atctggatga aaatggcata
    19321 acgctccgaa aaggtgacat tcgagtgtta aaacaggtac aatagctgta ccgattcaac
    19381 cattcacaaa gaggagagaa cataatggca tggaaagtat tgataaccga tgggctggag
    19441 agcaatggca aagcaattct gactcaatcc gatatcgctg ttgataagaa gggcattgaa
    19501 gcggcggaac tgctgcaaat catccccgaa tatgatgcca tcattttgcg cgggcgaacc
    19561 aagatgaccg ccgaagtaat tgcagccgca aaaaacctca aagtaatcgg acgcatgggc
    19621 gtcggtgtgg acaatattga cctgaacgct gccaaagcgc acaacgtgac cgtcgtgaat
    19681 gccgcggtgg caacctcgct tgccgtggcg gaactggccg tgggcatgat gttcgcgctc
    19741 gcgcgagaaa ttccccgtgc ggatgccagc ctgaaagccg ggcaatggct caagaaggac
    19801 ttcaacggca tggaactttt tggaaagacc ctgggcgtgg ttggtttcgg taacatcggc
    19861 caggtcgtca gcagatacgc gctggctctg ggcatgcagg tgctgtcgta tgacccctac
    19921 cgggacacga tgtatatgca cgaacgcggc gtggaagctg tgagtctgga tggactgctg
    19981 gaacgcgccg atgtgattac cattcacacc cccctgacgg accaaaaccg gcaccaattc
    20041 ggagacgcct gcttctccag catgaaagac ggcgtcttga ttgtggatgc tgcccgcggc
    20101 ggcattatcg atgaagaagc cctcctgcgg gcgcttgaaa gcggcaaagt gcgcggcgcg
    20161 gctctggacg tttacgagaa agaaccgccg gtcagctttg acctgataaa gcatccccgg
    20221 gtaatcgcga ccccgcatat cggagcgcag acctcggaag cgcagctgcg agcggctgaa
    20281 gacatttcgc atgaagtcct gaatgcgttg aacggactgc ctttgcgctg gaagatttgt
    20341 tagccaatgg gcagcgaaga gaaactggca aaactgcggg aaatcaccgc cgaaatcaga
    20401 cgcctgggtt ccattcaggc gctgctggaa tgggaccagc aggtgaacat gccgcgcggc
    20461 ggcgcggagg accgcggcaa ccagacagct ctggtcgccg gtctggcatt cgataagagc
    20521 acttccgacg aacttggcgc gctgattgaa gatctagccg ccgaagtgcc cgacctggac
    20581 ctcgatgacg attttgcccg cgaaatcaag gtggcaaaac gctactacga ccgtaacgcg
    20641 cgcataccgc gcgaaaaaat catcgaattt gtcctggcga cttcagcagc gcatgaagcc
    20701 tgggtgaagg cccgcgccga aaacgatttc gccagctttg aaccgcacct gacccacatt
    20761 tttgacttgc ggcggcagca ggccgagctc ttccaaccgt acgagcatcc ttacgacccg
    20821 ctgctggacg aatacgaacc cggcatgaag accgccgacg tccgcgcgat attttccgcc
    20881 ttgcgtccgc gccaggtgga actgctgcgc gccatctctg aatccgaaca ggtcgacaac
    20941 agcttcgtca agcagcatta tcctttcgag gagcaacgca aaatcagcag cttcctcgcc
    21001 gggcgcttcg gctacgattg ggagcgcggc aggctggacc tggctccgca cccctttacc
    21061 accagctttg gacatgggga cgtgcgcatc accacgcgct tcctggacat ggacggcatg
    21121 tctgccctct tcagcaccat gcacgaaacc ggccacgcgc tctacgacca gggttactcg
    21181 cccaggtacc gcgaaaccag cctggacgga gcggcttcat tagcgatgca cgaatcccag
    21241 tcacgcctgt gggagaattt ggttggtcgg tcacaggagt tctgccgttt cgttttcccg
    21301 gtcatgcaaa tgctcttccc gcagcacctg ggcaacgtct cgctcgagca gttctaccgc
    21361 ggaataaacc gggtggaacc ttcccttatt cgcgtggaag ctgacgaagc aacctacaac
    21421 atgcacatca tgctgcgttt tgaaattgaa accggcttgc tggaaggcag cctgcgcgcg
    21481 cgcgacctgc cggaaatctg gaacacccgc atgcaggaat acctgggaat cactccggcg
    21541 gacgatacca ccggcgttct gcaggacgtg cactggtcca gcggcttggt cggatacttc
    21601 cctacctatg ccctggggaa cctcatttcc gcgcaattat gggaaaagat ggtcgcggcg
    21661 catcctgaaa tcccaggggc aatgtccgca ggcgacttca gtcaaatcct cgcctggctg
    21721 cgggagaacg tgcaccagca tgcctcgcgc tatgaccctc aagacctggt gcgaaaaatc
    21781 accggcggac ccatcgaccc ggaaccatat ctgcgctatc tgaacgcgaa atacagccgg
    21841 atttatcggt tctgagcaca gaaagaaaaa taaaaaacac gaccgcgtgg acggtcgtgt
    21901 ttgcttttaa gcttaatgcg ggaggtttgc cgtgcttacg gtccggtgct gacgccgcca
    21961 tagaaagccg ggtccgtcgg ggtttttccg ctcgggtcgt tcacgtagac ccaatcgccg
    22021 acctttgccc aatggtaaac ccaattcgcg tccccggggg acatgttcac gcagccgtgg
    22081 gactggttgt acccgagcac caccggccag taaacgccgt ggatggctgt gttgcggtca
    22141 tagaacatcg cccagggcac ggcctgcagg tggtaaaaat ccgagcggtc cgcctcatag
    22201 gcatcctgca tcgtcacggt ctcgtacatc ttatagatct gaaaggttcc gggttgggtg
    22261 taaaaaggcg gcagtccgct ggcaatcagg ctggcgaaaa ccagctcccc gccctcatac
    22321 accgagagcg tgaagttgcc caggtcgata tgaatccagc gatcgccggt aacgccttcc
    22381 ggtcgggtag tatccaccac catccggcgg gatttatgcg aagaaaccca ttcaacctga
    22441 ttgatgcggt accactcgta gccatctgct tccgccacct ggtagatttg gatcacttca
    22501 tatttggcat gcaccttgcc cgtcttgggg gaagcgtagc caggcgcggt gtaagattcc
    22561 gcgtcgttca caatccagcc gaagtcgttc ctgggcgtgc gggaaaaccg cagcccctgg
    22621 aagcgcgtcc aggaatagat aggctgggct tgcatccact ctccactttc caattgtacg
    22681 agaatgcgcc cgccgtcgga cacctgattg accatcgaga cataacgcat cggaccggca
    22741 gccagggtgc gcagcggcgt tccgccgttg cgggcttcat ccagactggc atagaccggc
    22801 acggcttcgg aagtacccgc cacgattttc gccaccgata caggcagctc agtcagagaa
    22861 taatccggcg aagccgcggg cagttcttcc aggatgtcag ggatgccttc atcggccagg
    22921 tttttcaccg attgggaagc gcccagggcc aggcagtcat ccggcgcgct tggatagaag
    22981 ccgggcagac agagcggcaa gtcgtaatag ctgccgtctg tttcagcgga agctgcccgc
    23041 gctgtgcctg ccggcaaaaa cccgctcagc agcgcgggaa caagcagcag gaaactcata
    23101 ttccgtaaaa tcttcataca ggcattatac ctgtttcgca aacaggcgct caagggaata
    23161 caagctcgct gttttccggc agtcttgcca gggattaatt gaaaaaacgc agggaagaat
    23221 gccattttca ctcatttccc cactgaaaaa gaacttgcta cgcttataat taagatagaa
    23281 tcagaacatc cagaaatgag gagttcgcca tgaagatacc cgtctatcat gttgatgctt
    23341 tcgcagttga acccttccgg ggaaaccctg ccgccgtctg tctcttgcaa gccccgcgca
    23401 gcgtggaatg gatgcagaac gtggccgctg aaatggcact ttctgaaacc gcgttcctcg
    23461 ttcccaaagg cgaaggctac tatctgcgct ggttcacgcc caagaccgag gtggacttgt
    23521 gcggtcacgc caccctggca agcgcgcata tcctgtatga atttggcttt tatgaacctg
    23581 atgagaccat caaattccag actcagagcg gaataatcac cgccagcttc ttacgcggta
    23641 ccatcgaact ggatatgccc cggcgggacc cgagccccgc gcaggtctcc gcccaattga
    23701 gcgaggttct ggaccagaaa cctatcgcat cggccgtcta taaaaaccag gtcatcctcg
    23761 cggagctgga aaacgccgaa gcagtgcgta attttgtgcc ggatttcaaa aaaattgcct
    23821 ccctccccta tccggatgtg attattaccg ccccctgcaa ggaaaaatac gattttgtct
    23881 cccgcttttt ctcgccgctc accggaatcc cggaagaccc ggttaccggc atggcgcact
    23941 gcacgttggc tccgtattac gccgaacgca cccacaagac caaattcttc gcctatcagg
    24001 cttccgcgcg cggcggagaa gtctgggtgc gcctgggaag cgaccgggtg tatatcggcg
    24061 ggaaagctgt gaccgtttcc cagggggacc tgctgcatca aaaagattga gcaggcgttc
    24121 cccggaactg gttttacctt ccatcctgtg tgattcgacc gcagaagcgc tcagaagccc
    24181 gttttaccgg cttgaatgcc cgccaaccac aagcgcaagg agctcgaaca tggaactttt
    24241 gaaacaaaga atcttacaag atggaaaaaa tcttggctca ggcatcctca aggtggaccg
    24301 ttttatcaac caccaggtgg acccgctctt gatggacgca tgcggagcgg aatttgcgcg
    24361 cctgtttgcc ggccttggcg ctgaaaaggt gcttaccgcc gaaatttctg gcatcgcccc
    24421 cgcgttaatg accggcaaat atctgcacct tccggttgtt tatgcccgca aaaccaagcc
    24481 aattaccatg cccaacgagg tttacctgac cgttgcgccc tcgcacacca aaggcaacaa
    24541 cgtcgaattg attgtctccc ccgagtatct cggcagagag gaacacatcc tgattattga
    24601 cgacttcctc gcgtccggcg cgaccatcgc cgggttggtg cgcctggcgc aggtggccgg
    24661 cgcgcaggtg gtgggcatcg gcacgctgat tgaaaagtcc ttcgaaggcg ggcgccgctt
    24721 gctttcgtac ctgaatatcc ccatccattc gctagtgacc atcacatcga tggacgcggg
    24781 caagatagtc ttcgcggatt aacccggcag cccaggaaat gaccgataaa atcatcatcg
    24841 ttgactacgg ctcgcaatac acgcagctga ttgcccggcg cgtgcgcgaa gcccaggtct
    24901 acagcgaaat cctggcctgg aatgacccca aacttgggca ggcggacccg gaaacccgcg
    24961 gttttatcct ttcaggtggt ccgatgtcgt cttatgaaca aggcgcgccg ggcctgcctg
    25021 ctgcgctgct ggggagccat ctgcccatcc tgggcatctg ctacggcatg caggcgctcg
    25081 cgctcgcgct tggcggggag gttcgccgcg ccggcgaaca tgagtacggg cttgctgaaa
    25141 tccgcgttct gaaaaataac ccgctcctgc caggaagcag ccagacggtc tggatgtcgc
    25201 atggggacca ggtcacgcgc ctgcccacgg gttgggaagc gctggcaagt tcagttaact
    25261 gcccgattgc tgccgccggc gactccgcgc ggcgcttatt cgggctgcaa ttccacccgg
    25321 aagtgcgcca caccccgctc ggcactgaga ttttgcgccg ttttgtgctc gaaatctgcg
    25381 cctgcgccgc gacttggacc ccggaatcga ttatccagca aagtatcgcc cggattcgcg
    25441 agcaggcagg cggcaaacaa gtgctatcag cagtcagcgg cggggtggat tcgagcgtgg
    25501 cgaccgcgct gacgcgcgcc gcgctgggcg ggaatgtgca cagcgtcttt gtggataccg
    25561 gcctgctgcg cctgggggaa cgcgaacagg tggaacaagc cttttccagc ctccccggtg
    25621 agcctctgcg tatcgtggac gcgtcggagc gtttcctttc caaactgcgc ggcgcgaccg
    25681 acccggaaca aaaacgcagg attatcgggg aagcattcat ccgcgagttc gaagccttcg
    25741 cccgggaagt cggcagcccc aggtttttgg tacagggcac catttacccg gacgtggtgg
    25801 aatcacaggg catcggcgcc tcccaggcag agcgcatcaa aagccaccac aacgttggcg
    25861 cgctgcccaa ggatatgaag ttcgcgctga ttgaaccctt gcggctcttg tttaaggacg
    25921 aagtgcgggc ggtgggcgaa gcccttggtt tgccgcgctc gctggtctgg cgccagccgt
    25981 tcccgggccc gggtttggcg gtacgctgcc tgggcgaaat tacgccggag cgcctggaca
    26041 ccctgcgccg cgcggacgcg attttcacct ccgaacttga acgggaaggg ctgctgaccg
    26101 cggcggaaaa cgcacaaggg ctgccttccg gcagttccca ggcatttgcc gtgctgctgc
    26161 ccgtccgttc ggtcggcgtg atgggcgacc aacgcagtta tcatgaagtc gttgctctgc
    26221 gcgcggttac taccacggat tttatgaccg cggattgggc gcgcctgccg tacgagctgc
    26281 tcgcgcgggc agccatccgg attgttaatg aggttccgcg ggtgaaccgg gtggtgtatg
    26341 atatcaccag caaaccgcca gccactattg aatgggaata atcccgaaat aagtgaggac
    26401 ctatggatta cgcagaaatc ctgaaaaaag cctggaacat catctggaag cataaaatcc
    26461 tgtggctgtt tggcctgctg gcaggttcgg ccatcggcag cggtcagggc ttatcctatc
    26521 gcttttcatc tcaggatcgc attcagcagt tttggcagaa caaccggggt ttcccgtctg
    26581 ttttcgagcg cgtgagcgaa ttctatcgca gcctgccgcc ctttgcctgg tttctgatcg
    26641 caggcgctgc tgttgcggca gtcctgttta tcagtcttat cctgtttttt gtgcgcgagt
    26701 acggcctggc cggggttatc aaaggctccg ctttggctga cgagccgggg cgcagaggtc
    26761 ttaagttcag cgaggttcac cgggccctga agccgtacta ctggcgcctg gtcttgctgc
    26821 gcctcctggt agtctgcgcc ggcgcgatac tggccggctt actggcaatg ccgaccatca
    26881 tttttatcat cggcaccctg ggcatcgggc tgtgctgtct gattcccttc ttcatcctgc
    26941 tcattccggt cggatgggga gcaagcgttt tggtgcacaa tgcctgtatt gccttggtgg
    27001 atgaaaacct gggcgtggtg gaagcgctct cccgcgcctg ggatgtgacg atacaaaaca
    27061 tcggcccgct gctggtgctg cacctgctcc tgggcgtcgt ccgccttgtc acaggtctgc
    27121 tgatgagcat tcccatgctg gccgccgcgc tcccgctgat tctggcgctg cgctccccgg
    27181 gcagcgcctg gccgatagcc gccggattac tcaccggcct gctttggctt atccttcttc
    27241 cgctggggct ggcagggatg agcatcttaa acgccttcga cctgagcgcc cgcgcgttgg
    27301 ctttccgcga agtgaaggac aaagcgcgcg tcctgcccgc cggaagccag gagctgccca
    27361 ggcaggaaac gaattaggaa catgagctta cctgcccttt ctccaccggg ggcggcttac
    27421 cacgcggcgg aacatcgcgg aggactgctc ccgcgcggat ttttaaggcg ctgctactgc
    27481 gccctggttt tcttcctgct ggtattgcag cccgccctgc cggccaacgc ctcctacccc
    27541 gggcaagcct cacccatgct ggaaatcctc aacatcgata cgcgtgagtt tccgcttatc
    27601 cgggctgaaa taaaacccga taatctgcct gtcggcctgg cagttccgat aaacgccggc
    27661 acgctcagct tgttggaaaa cgggaatacc atccccgcgc gggacgtgca ggagaaatcc
    27721 cagggcatct atttcgcgct gacggtcaac ccggattttg cgctggattc acgcgacccc
    27781 aagggcattt cccggtacgc caaacttgtc ggggcgttca aacagatggg aagcgcgttt
    27841 tctgcagagg gggttgaccg tttcagtctg tttatcaacc cggactacgt ttttgaacag
    27901 ctttcggact ttggctccct cgttaccgcg ctggagggct acagcgagaa cttccgcacc
    27961 cagaagtccg acctcaccag cctctcccgc gcgattgacg cgctgagcat ggacgaaagc
    28021 ggcaaagata aggtcctgct gtacatcacc ggactgccca ccatacagga cgcaaaaagt
    28081 attcagctgc tttctgaaat agcgcgggaa aagcatatca aggtcatcat ttggttggca
    28141 ggcgaggcct ttatcgccac ctacccccag attccctacc tgcaggacct ggcagacagc
    28201 agcgccggca gcctgtttct ctattcgggc agtgagccgc tcccggaagc cgacacctat
    28261 ctgaacaatc tgggcagaac ctatctggtg tcctaccttt cgctcgtgcg ggaaagcggt
    28321 acccagagct tgagcgcgcg cctgcagttg gaaagcagca cgctcagctc aactagcagc
    28381 agcttcgagg tacaggtcga gcctgccgag ctgagtttcc ttaacctgcc gcaggagcta
    28441 accctgctgc agtccgcgga cggcgctctc acgcctgcag aactgccgct tgaggtgctc
    28501 gtggatttcg tggacggcca cccgcgcaat atcacttccg cccgcctgct ggtcaacggc
    28561 gctgacgtgc aggagaacat ccaggcacct ttcaacagtt ttgtactctc cctcaaggac
    28621 tatgccggca aagacaagct ggctctgcag gtgctgatgg tcgatgacct gggtttggag
    28681 gcccggaccc ccagcacgga aatcgcactc agcacgattt tgccgtcaac aggcggcgcg
    28741 ggaatattcc gcagcccctg gctgctgctc acagcaggcg cggcactggc tgccttggta
    28801 gccctggtac tcctgccgtc gctgcgcaag gccaaaagca aacccctggc aagcctaagc
    28861 gctgaagctg ccctgcctgc cccgctgccc tccccggcga ttctggcgac gctgaccagg
    28921 ctcagcccgg agaaccttcc cacacctgaa aagcccatcg cgattacaca ggaaatcacc
    28981 atcatcggcc gcgacgccga cctatgcaat ctcgtcctgg acgacccggc ggtcgaagcg
    29041 atgcactgcc agctgcgcaa gctccctgac ggtgaattcc gcctgacgga ttttcgcagt
    29101 gcagcgggca cctgggtcaa ttacgcgcca gtcggagcca aaggcatccg gcttcagcat
    29161 ggagacctga ttcaattagg ctcgcaaacc ttccgcttcg gcagcggttc acgcgtagcg
    29221 gctgtggaaa agaaccaggc gcatagagac ctgacagacg gcagttcacc agcggaataa
    29281 ggcataacaa tcgcggctat tcgcggagag ccgaaaacaa gagggctgct ttcccgaaat
    29341 cgaagggtgt ggataaagga tcaagaaaga gcttaccaac tctcgagagt ttacccaagg
    29401 gaccaaaact ttctgacgag caagcgtgcg aacagccttt cccctcttac tttctttcca
    29461 cgcggactca atttatccta tgaccctgat gaactatcgc tgcgacgggg agttatcatt
    29521 tgccagggag ttgtgccgtc tatccatttt ttctcttctc cagaagattg atcgccctgc
    29581 aaaaaaccgc ttaccgctgg gaacgggtca ggcttagggt acccgcggca catattcgct
    29641 ggttacttag aggggtgaca taatcgctga ctaacaacat ccgcgagaag tttgcttgag
    29701 aagttgctct tacacgcgag aagtttgctt gcccatagat ttgatatatg ttagaatagc
    29761 tttcctacgg agggatggcc gagcggctca aggcgcctgt cttgaaaaca ggtatgggga
    29821 aacccatcgt gggttcgaat cccactcctt ccgctcgggt cgacacccgt atatggggag
    29881 gtgccagagt ggacgattgg ggccgcctgc taagtggttg ttggagtaaa atccaaccga
    29941 gggttcgaat ccctccttcc ccgcctggat atcgcgaaag cacctgcaaa ggtgcttttt
    30001 gcgtttaacc ctgggggaac cccatccggt cgttatcact gcctgttgcg taccagcccc
    30061 aaaaaacaga tgcgcttttt acagccggca gttctgccat gcttacgcgc cgcggatcat
    30121 ttttatatct cccccactcc ggaaggctgt cagtgatggt cagaatctcc tgcaggatta
    30181 gcggctcatt ccatgccttc ttagcagcaa gggaaattca gtttgctttt tcataagtat
    30241 cgagcagttc tggcgcgatt attgcaacag tgtacctgat gaaatggctt tgtacgaata
    30301 caggctgtcg gccttacgcg ttaacatagg catatccctg tggaactctg cgcatttccc
    30361 gaggaatgac gcaagtcttt ccgaacacgg caggcctgac tgccagcgag cgccggaagc
    30421 taaacctcca cagggttttt gtccgcaata cgattgcact aaaggagtat cgcatgaaaa
    30481 aagcaaaggt tttcctgatt atcatagtta tcagcctctt ttccgtttcc ctgttggggt
    30541 tgaaaggacc gcagattgcc aaagccgaag acgagaaccc gcctgattca gagctgattc
    30601 agcaaaatgc ctggctgctt ggcacagaac cagcccaatc tgcttccagc gcgactgttt
    30661 tttcgctttc aggatgcaac caggagtttt gggataatgg tgaattcatc acccacccgg
    30721 gcggcggcta ccagggcgcg aacgtctccg cgctgcagac tgcgcttgga atgcagactt
    30781 acggctttac caaccagtac ttttctacag gctatcatac cgcggacgac tttatcatca
    30841 cggatacgcg cggttggcag ctggataata tctccttctt cgcctatcag gacggcgact
    30901 atccctttcc tccttcctcc ccgcttgtgg aagtttacct gcaaatctgg gacggtccgc
    30961 caaacgaccc caacagtacg gtggttttcg gcgacctttc caccaaccgt ctgatttcca
    31021 cttcctggac caaaacctac cgcgcagctg acaatagttt gatggatgac agcatgccta
    31081 tcatggaaaa cgtcgtctac gtgggcgcgt ttctgcccgc cggcacctac tggctggatt
    31141 gggcaaccaa aggcagtatg agcaccggac cctggattcc gccagtgact gaactggggc
    31201 agactacaac cggaaatgcc tgggtgttta aggattcctg gcagcccgcc ctggacgcgg
    31261 gcaccggcac aggccaggac ctgccattta tcatccgcgg ctgccgcgaa caaggaccct
    31321 ggcagcagct ttcgaacagc caggtggacc gcatggacaa cgtcctggct gcttatgatg
    31381 gcaaaatctg ggacgtcacc ggccgtggtg agactggcgt ctcaagctac gaccccgcca
    31441 ctggtctgtg gtcgctggtg agcgcttcca cgccgccctt cggcagcaac tacgcccgct
    31501 ccggctgcca ggtcgggaat gaagtattta tttacggcga cggcgttacc gcgggcttta
    31561 ccggcctgtg gagttataac ctgagcacaa acctctggaa gcaggaaacg ccaagcggaa
    31621 acccgcctgc ttaccaaggc atctgggcgc cagcctgggc agcggacccc gcgaccaaaa
    31681 tatgctatat gaccggcggc gcgcataccc ccggcggcgg caccctcacg agcgtttatg
    31741 tctacgatac catgcaggac aaatggctga ccccgctgcg cgctttcgat agcgtacgcg
    31801 actaccacgc cgctttcctc ttcacccgcc agagcgacag ccataagctg ctgtgcgtgg
    31861 tcggcggcgt tacaaccgga ccggttctca cgaccaccca gtgctacgac ttctccaagg
    31921 ctaactggaa cgaagaagat atcgacctcg gcccgctgcc catcgcgctg tggggcatgg
    31981 gctacgcgca gaccaccatc tccggtgagg tccagctttg gctcgtaaac ggcgcagacg
    32041 gcgggttcaa tatcagcccg gaaacctgga tattctccgt caaaaccaaa acctgggcga
    32101 gcgctggttc gctgcaaagc ggcgacttcc tccgtacttc cgcggtgact atggataatg
    32161 tcgtctacca cgtcggcgga tccctgggcg gttttaacta tgccggaaaa gccgataagt
    32221 atatcggttt caggtcatac cttccctgcg cttccaagtt ctcacagctg aagtaagcga
    32281 aaaactgttt cgggtgtcga acctcaaaag agggctgtcg taaaagtcgg acagcccttt
    32341 ttggtttccc actccgcgcc cgtttttatg gtcggttgcg gatttgcgct atcaaaacga
    32401 ggcctaaacc aggcacgttc ttccactctg ggctgtccaa gaacgaaaac cctttctttt
    32461 tggacagccc ccacccattc agcaagcgaa acggcgccaa ctctgatggg ctacatttcc
    32521 agccaaaagc cgggcctact cgtccagcaa acgccgttca ccttgcaggg aacgcatcgc
    32581 gctgacatcc tgcgtaacct caataacgcc cttgtaggtg ccttgctcat cccgcagcgc
    32641 aaagtagcgg atgtacacaa acttgccgcc catctgaatc cagaactcag ccgcgtcgcg
    32701 tttgccagcg cgaaaatcat ccaggatgtg ctgcacaaca tgcacgctct gcggcgggtg
    32761 gcagttttgc acggaccgac cgataatcgc cgcggtccgc acgaaaacgc gctccggggt
    32821 ctgcgaaaag aagcaaacct tatcattttc atccacgaaa gtgatatcca ccggcagctt
    32881 ggtgagcatc aggttaatct gttcctgggt cagggcgcca gtggaaagct tgattttccc
    32941 ctccgctgcg ggctctggcg ccggttcttc ccgggctggc tcagcgtctt ttttcaggct
    33001 caggctttcg gctttaatca tgtcccaatc tgcttgtgtc aggcggctca gagattccgg
    33061 cagcagcact ttctcttcct tgtagaacat atcccgcacg gcttttgcca ggtcaagata
    33121 ctccgcgtca acggtctcaa ggtccaatgg atcgctgttc atcagggact gcaggttgcg
    33181 gatggctttg cggatgtcgt tgtgtacgcc ccacatcacc ttggaaggcc cttcgaaccc
    33241 gtatttttcc agcacaggga agagcaggtt ctctttccac tcgtagtggc gctcaatcag
    33301 gttcaggttt ccagccgcct ggcgcaggga tttcagcgcg ttcttatccc cggccagcac
    33361 gcgcgccagg ctgccttcga ttgattcgag cacgcggctg atgacctcgt tctcgagccg
    33421 gaagttatac accgggtgac ctggcacaga ttccggcgcg gtctggtttt ccagaccctg
    33481 ctggaagacc gcgacatgca agtcgcacag tttctgaatg tccgctaccg gcagcccttc
    33541 ggcaatcact tcgcgttccg cctgggcaac gtccgaagcg ctggcttcag cgataacctc
    33601 gttgaactct tcctgcaggg attcgacgct ctccccggca tgcagcttgc ggataatggc
    33661 cttaagggca tccttgcgtt tggacgcatt gttgataaac tcgctcataa taactccttt
    33721 gtgctattct tctgtgcccc aactgatggt cagcgggcat tggatcgcgt gttgcggaca
    33781 aacttgctca cactcggtgc agtaggtgca tgcatccgct tcagcaaaga caggcgtcga
    33841 attctcagcg tccatcgcaa gcgcgtgctc cgggcaggcc gcggcgcatt ctccgcagcg
    33901 gctgcacaga gccaggtcaa tcaccggaag agcatgcatg tttgtcatag aggagagttt
    33961 actgtgtatt ccaaacctgt gctgtgactt aagtcacaac tttgcatggg aatctcgatt
    34021 cctggggaga ggaaatgggc aagctcacca gcgctgcgct tcttcctcga gcgccgcccg
    34081 gtttttcaat accaggtgct gccgcttgcg ttccagccaa cccgcccggg caaagtcgcg
    34141 cagcagccgg gcgacaattt cgcgcacggt cccgatttcc gccgcgattt cctcctgggt
    34201 aatgccagca ggctgtccgc cctggtcggc ttgcctgatg agaaaagctg ccagccgcga
    34261 gcgcgcgctt tgcagactga ggctggctgc caaaaagctg agttgttcca atctgcccgc
    34321 caggcagcgc tgcagagcgc gatgaaactc cgggctgctt tccgcaatct cgtggaacag
    34381 ttccagactg atagagagga ctttcgtggg gtggctagcc cgcccgctct cggtatggca
    34441 ggcgtttttc tgcagcagga gcggggccag ggaaataagg tcccccgcct ttaagtgcgc
    34501 gagcacctgt tcccggccgt cggaagaacg cttgaccgcg cacacatcgc cttccagcac
    34561 gagcagcagg ctgttaatct catccccctc gcaaaaaacc tcctcccggg cgccatagct
    34621 gtgcaaaacg ctgctcttcg ccatccggag caaaaggtcc gcagggagca tggaaaacag
    34681 ctcgaactgc cccaggcgcc gggccaggtc ctgttccgcg ccggtcacag cacagcgctc
    34741 ccctgctgtt tccgggcgca gaggccaaat atcctggcaa gcacaatcag tcttgccatt
    34801 cttccgcctt tatcaccgcg cgcgcattcc attttcgggt taatcatacc cgtttccggg
    34861 cacttcgtgg tttttcgagg gaaaaagctt cttaaaagga aaaaacttag ctataatact
    34921 tatagtggtt tgacctctta ccaatccgcg cccggaggaa tcaatgcctg aatggagccc
    34981 gccgcaaaaa gcggcagaaa tcacggaaat tcgtctgcag gatgctattc tggacggcac
    35041 tttcccaatt aacagccatc tcccccccga acgtgtactg gcagaaatgc tgggggtcac
    35101 ccgcccgacc ctgcgcgaag ccctccagcg cctctcccgc gacggctgga ttgaaatccg
    35161 gcacggcaaa aacacccgcg ttaagaatta catgcaagaa ggcaacctgc ttatcctgaa
    35221 ctacctggcc aaaaacccgc aagcccagcc gccctcgttc gtgcctgacc tgctggatgt
    35281 gcgcgccacg ctcgcgccgg ttttcacccg ccaggcgctc ctgcggaacc cggaagcagt
    35341 ttgcgagcac ctggaccaaa tgcaggagat cccggaggat gcccgggaat ttagcgcggc
    35401 tgacctggaa ctgcagcgcg tcctcaccat tctctccggc aacccgattt acaccctcat
    35461 cctgaacggc ttcgcggaac tgtaccgggt gatgggtccc ttctactttt cctcgcctgc
    35521 agcccgggag aggtcccgca gattttactt tgaattgcgc gaggcagcgg aaaaacgcga
    35581 cgcggaagcc ggcgaacgcc tgacccgcga gatgatgaca gagagcatcg ccatctggaa
    35641 ggacgcacgc aaacaaggag agcaggcaaa atgaacgcga acaagagccg ggctgagacc
    35701 tggcaaagcc ttcagcagga ttgggacatc atcgtcatcg gcggcggaat cacgggcgcg
    35761 ggagttttcc ggctggcggc tgcgcagggc ttgcgcgtgc tgctgctcga acagaacgat
    35821 tatgccagcg gcacttccag ccgctcttcc aagctcattc acggcggctt ccgctacctg
    35881 cgcaaccgcc agtttgatat cacacgcgaa tcggtgcggg aacgggaagt tctgctgcgc
    35941 gaagccccgg gtctggtgcg cccgctcggc ttcattctac cctacaccga ctccgggacg
    36001 cagcgcgcag agttccacct gggcgtcacg ctctacgacc tgatggcgcc caaatggaag
    36061 caccgcggcc tctcccaaac ggatgtacac agtctggtcc cgcagctcag cgccccctgg
    36121 ctggcaggcg cgtatttgta ctacgacgcg gtgatggacg acgcgcgcat ggtcttgcgc
    36181 ttgctcagcg aaggctgccg cgcgggcggc acagcactga attatgcccg cgtagaaaag
    36241 ctgcttacta cccgggaggg cacagtctgc ggcgcagccg tcagcgacca atccccgcag
    36301 gccttgggca gcctggagct gcacgcgcgc gtgcttatca acgcggctgg tccctggtcc
    36361 gacatgctgc gcgcccaggt gaacgccccg acccgtctgc gcccgcagcg cggctcccac
    36421 ctggtttttc cgtacgagaa actgccgctg ccgcatgccg tcacactgct gcatccgaaa
    36481 gacaagcgcg ctatgtttgc catcccctgg gaaggtacga ccatcatcgg cacaaccgac
    36541 ctggaccatc cctccccgct ggatgaaggt gagccgtttt gcacccaggc ggaagtggac
    36601 tacatcctgg aagcggcggc agccttcttt ccctccgccc ggctcagtca ggcagacgcg
    36661 ctttccagct ttgccggttt gcgcccgatt atccgcggaa acgccgcgaa cccttcggcg
    36721 gagtcgcgcg cgcatgtggt ctgggaggaa aaaggcctgg taacaattac cggtggaaag
    36781 ctaacgattt tccgcagaat ggcgcaggac gcgctgcgca aagcagcgcc ccggctgagc
    36841 ttgaccctga aaagccacgc aagattctac gacgcccttc cccgccaaag cggagcggac
    36901 agcacggaac tcaaccccgg gcagcgctgt gttctcgcag gaaagtacgg tttcgcgctg
    36961 caggaattgc tcagctgtgc aaaacccggg gaattacgtc cggtgcaggg gctgcctacc
    37021 atccgggcgg agttgcgtta cacggcacgc agggaaagtg tgcagcacct ggatgatttg
    37081 ctcctgcgcc ggacgcgcct ggggctgctc ctgccggaag gcgcgcgcgc ggaacttccc
    37141 ttcctacgcg aaatcacacg ggaagagctg gactgggatg acatgcgctg ggaagccgaa
    37201 gtccagcgtt acacccaaat ttacgagcgt ttttacaatt ccgcgcccgc tggaatttag
    37261 tctttcagga ggataaaaat ggcaaaaaaa gaagtcttta cgcccaactg gtacgaagac
    37321 gtcaccccgg tcggcacata ccgatctctg ttgaaatggg gcgaccccgc cggcatcaaa
    37381 cacccgaaca gcggcctggt gcgcctgatt atggacaaat tccagctcac ggaagcggat
    37441 ttgcgcgcgc cgctcgacct ttccctggag caggtggagg acagcgcgcc ttgccgcctg
    37501 ccagaagagc atctggagtt tttccgcgcc ctgtgtgggg atgaaaacgt atgcaccgat
    37561 acctatacgc gcatcgcccg ctcttatggg aaaggcatgc tggacgcgct gcgcttgcgg
    37621 cgcaaaatta tccaaaacct gcccgaaatc gtggtcgcgc cgcgcagccg ggaagaaatc
    37681 gcacgcatcg tagcctatgc tgacgagcac cgcatcccgg tgaccgtctt cggcggcggc
    37741 tccacggtta cgcgcggcat ggaaaccacg cgcgggggca taacggtgga catgagcaaa
    37801 cacatgaacc gcgtgctggc gtttaatgag accaaccaga ccatcaccgt ggaagccggc
    37861 atgagcggac cgaagctgga ggcgctgcta aacaacgccg tgcacgagtt tggtgcggcg
    37921 cgcgcttaca cctgcgggca gttcccccaa tctttcgagt attccagcgt gggcggctgg
    37981 gctgtcaccc gcggagcagg gcaaaactcc acctattacg gcaagagcga agacctggtc
    38041 atttcccagg attacgttac gcccatcggg gattttcgca cctgccctca gccgcgcgcg
    38101 gcgaccggac ctgattttga ccagataatg ctgggcagcg aaggcgcctt tggcatcctg
    38161 gtgagcgtca cctttcgcat tttccgctac atgcctgaaa accgcaagcg ctttagctac
    38221 atgttccgca cctgggaaga cgcccaagcc gcctgccgcg agattatgca agccgaaatt
    38281 ggctaccctt ccgttttccg cctctccgac acggaagaga ccgatgtggc gatgaagctc
    38341 taccatatcg aagggaccgc ggcggatacc attctgcaaa agctgggcta caaaccaatg
    38401 caaaagtgcc tgctactggg ttttaccgac ggcgaaaaag gtttctgcgc caacctggac
    38461 cggaaaatcc gccggatttg caggcagcac ggcgcctttg agctcagctt gtttggtgtt
    38521 accaaaagtt gggaaaagga ccgcttccgc gacccttacc tgcgcgagga tttacaggat
    38581 ttcggcgtgc tgattgacac actggaatgt tccgtcacct gggagcagat gccgcgcgta
    38641 caccaggaag tgcgcgcgtt cgtgaagagc cgcccgcaga ccgtgtgcat gacccacctt
    38701 tcgcatgcct acccccaggg cgccaatcta tactttattt ttatcgcgcg catcggtaca
    38761 ataaaggaat atctgcagct gcagtacgga attctggaag ccatcgcgaa aagcggggct
    38821 gccatcagcc accaccacgg cgttggcaag caaacctcgc cttggctgga ggagaccatc
    38881 ggaaaaccgc agatggatgt catccggctg ctgaaaaaac attttgaccc gcacaacata
    38941 atgaacccgg gcggcacgct tggcctggac atgagcgctg aacaaagaga aaaacgctgg
    39001 agtatggatt tggagggctg atgagctctg aacaactgct ggctatcgac aacggcacac
    39061 agtcggtgcg cgcgctgatt ttcgacctgc gcggaaacct gctggcaaaa agccaggtgc
    39121 caattgaagc ttactattcc gccgaacccg gctgggcgga acaggacccg caagtattct
    39181 ggaacgcgct gtgccaggcc tgccaggcgc tgtgggcaat ccccggcgtg gagaaaagcg
    39241 cgcttgccgg cgtcgcgctc accacccagc gctccacgat cattaacgtg gatgcgcacg
    39301 gtcagcccct tcgcccggcg ataatttggc tggaccaacg ccgcacgcct ggcctgaagc
    39361 cggtcggggg catttggggg ctgctcttcc ggctggcagg catgcggggc acagtcgcct
    39421 acctgcagtc ggaagccgaa gccaactggc tgcgcaccta ccagaaagat atctgggaaa
    39481 agacccacaa gtacctgctg ctctccggtt atctgacata ccggctcacc ggtgcgtaca
    39541 tagattccat cggttcccag gtaggctatc taccctttga ctacaaaaac ctgcgctggt
    39601 cagctaagtc ggactggaaa tgggaggctg tacctgtgcg cccggagacg ctgccggagc
    39661 tggtgcggcc gactgaattg ctggggcaaa tcagcgcgga ggcctccgcc gcgaccggca
    39721 tccctgaagg gctgccgctg attgccgccg cggcggataa ggcttgcgag gtcatcggtg
    39781 ccggctgcat cgaacccagc accgcctgcc tttcgtttgg caccactgcc accgtcaaca
    39841 ccacgcacaa agattacctg gaggtcatcc cgcttatccc accttacccg gcggcaatcc
    39901 cgggtatgta ctcgctggaa atccagattt tccgcggcta ttggatggtc tcgtggttca
    39961 agcgcgagtt tggaatgaat gaagaacgcc tcgcgcagga gcggggcgta aaaactgaag
    40021 acgtgttcga tgagctgatt aattccgtgc cggcgggttc gctcgggctt atgcttcagc
    40081 cctactggag cccggggctg aagcagcccg gaccggaagc caaaggcgcc gtgattggct
    40141 tcggcgacgt gcacacccgc ccgtatttct accgctccat catcgaaggt ctttcctacg
    40201 cgctgcgcga aggcgcggac cgcactaacc gccgctcgcg ccagaaaatt gaatcgattc
    40261 gcgtcgccgg cggcggttcc gccagttccg cggcggtgca gctgaccgcg gatattttcg
    40321 gtttgccggc atcccgcccg catgtcatcg aagccagcgg actgggcgcc gcgatggatt
    40381 tggcggtcgg gctgaagctg cacccggatt ttgagaccgc tgtacgcgag atgacgcgca
    40441 tcagcgaaac ctttgagccg aaccccgaaa accataaact ttacgacgaa ctctatcagc
    40501 gcgtgtacct gaagatgtac aaacgcctgc gcccgctcta cgaggaactg gcggatatca
    40561 ccggttaccc cgcgctggat taaacagaac acctcgaacg cccgggcgtt ttttatcctg
    40621 gtcttctaca aagccccttg attggtttcc agagggatag ttctttgctt ttcattgccg
    40681 gggtttatac caaagacagg tctattcgga tggtcgagac ccccatccca acctgtcaag
    40741 gctaaataga aatgtcctgt tgaggaaaga aagccgtcag cacgcctttt tcctcgaaac
    40801 gaatcgcttg atttcttctt tgcgatcatg tttcacgctt gccctcaccc tgacccgtcc
    40861 ccagcgtctt tttaggaagg atgatgggaa taatggaaat aacaaggaaa aagggaagta
    40921 aggggaaggc gcttgcaacg gttgggcttg cgcccactat tcaagttcaa ctcccgtccc
    40981 agcaaccggt aataggaaaa aacaataaag ttcagtcgcg gagaagggga gaaagagggg
    41041 cacctttctg tgtcagggta tgttcattcc tcggtaaaaa aattgtggaa tcgaatgctg
    41101 attcacagct ccaaaactta atcagggcct ttctattttg ctatttcagg acagtatccc
    41161 attgcgcatt ttcccctccc atccgtatat tggac
//
LOCUS       DAOQAD010000007        35629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_1088_length_35629_cov_5.599848, whole genome shotgun sequence.
ACCESSION   DAOQAD010000007 DAOQAD010000000
VERSION     DAOQAD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 35629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35629
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1088_length_35629_cov_5.599848"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKL60_01265"
     CDS             <1..213
                     /locus_tag="PKL60_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94793.1"
                     /translation="VPHDLRPCFVGLRCRSTRPTTMFCRVALPLHPTYDHVLSGCAAV
                     PPDLRPCSVGLRCRYTRPTTAAPHDL"
     gene            complement(742..2352)
                     /locus_tag="PKL60_01270"
     CDS             complement(742..2352)
                     /locus_tag="PKL60_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNW94794.1"
                     /translation="MNVQPHNLKAEEAPLSESETPAAAQTEGSHQPRPQRKGWHTQPG
                     SIAALLLILAALIILSAWLTSEPGDKSAAPTPTATALNAPQLALEEETAEPTATATAA
                     VLPTEEQARTEVITYTIQEGDSIFSIASKFNIAPETILWSNRYELGDDPAVYLPGVSI
                     YILPVDGVYHIWQAGEGLNGVSDFYGVTPDAIIDYPGNKLDRATLGNLSLPNITPGTR
                     LVVPGGTRPETVSADSLLTYAAATQIEKIPVGDPDTYPAPRKEIIAYVVQEEDTLFTI
                     ADKFGLTPETVLWANRYLLGDTPDGIVPGQKLFILPGNGVYHGWSYKENLDKVASFYN
                     VSRDVILDEPLNHLDRSAFADLSHPNIKAGTLLYVPGGTRPPVTWVTPVGPGEDGVGE
                     HPNVSYLGAFACNSTAWAVGTGSWQFPTSEHWISGYEYTPPTHNGLDYAGRMGYEIYA
                     ADSGVIIYSGWSTRGYGNTIVIDHGNGYLSLYAHLMDGGMIQGCGVEVYAGQLIGYMG
                     STGQSTGPHLHFEIRYDGSPVNPHDFGL"
     gene            complement(2599..4125)
                     /locus_tag="PKL60_01275"
     CDS             complement(2599..4125)
                     /locus_tag="PKL60_01275"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNW94795.1"
                     /translation="MKKSPGLFLLLSIALILGACANVTTAGPTSTLPAPQVQLSEEAP
                     PAVQAAPTEAESATTLVEVTPSTPSNPGAAIPEPENRTHYQLDLILNYYTQYAVVDEI
                     ITYTNRSSQTFSELAVVVPPKYYPGSFSLRGLTDGSGSPISGWQEDGVRFTVPLLAPL
                     APGQTTSLKFSYQLNLPAHEGTYGVSGRQTNLTNWYPYIPPYDEGTGWIIHPQQLENN
                     MQVGEYVVAEMADFDVSLRLTDRQELIEVAASAPATEKDGLRYYHLELARGFSFSVSD
                     SYFEHEIIQDGVRIHSYVFMEAQEAGKAVTEIAAQAIRFFGELYYPYPRDMISIVAAD
                     FLHNMEMDGMVMISYGVFNFYNKTPKTNLTILTPHEISHQWFYSMVGSDQAEEPWLDE
                     ALATYHEVLFYQRYHPDLVQWWWNNRVDGFTPKGYVNSDIYLSGGYPAYRDAVYLRGA
                     EFLQELRDLIGDKAFFAALKDYALTNTYRIATREDFFAAIARHSQEDLNPLLARYFHE
                     "
     gene            complement(4366..4788)
                     /locus_tag="PKL60_01280"
     CDS             complement(4366..4788)
                     /locus_tag="PKL60_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNW94796.1"
                     /translation="MEAKAVWQGDMSFTGSSDTGFSLKMDSNEVAGGHDDGFRPTELL
                     AIGTAGCTAMDVISILKKKRADVTAFEVRAVVAKAETYPKVFTRMHLIYTVSGHGIKP
                     EDVERAVQLSEEKYCPCIAMFRQVLELTREIKIIEAEA"
     gene            complement(4836..5069)
                     /locus_tag="PKL60_01285"
     CDS             complement(4836..5069)
                     /locus_tag="PKL60_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94797.1"
                     /translation="MKVSSPPETNEELPAQSFTSPESGRAEKALHVCGSSGPFSLRYG
                     LPCPFCQVGYVDYDSQLNLVCGNCGKTQTGAFT"
     gene            complement(5154..6188)
                     /gene="tsaD"
                     /locus_tag="PKL60_01290"
     CDS             complement(5154..6188)
                     /gene="tsaD"
                     /locus_tag="PKL60_01290"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNW94798.1"
                     /translation="MKAPQLRVLAVESSCDETAAAVVEDGREILSSIIASQVDLHAQF
                     GGVYPEIASREHIKAVYATVNEALQQAHLELSDVDGIAATRGPGLAGSLVVGANMAKA
                     LAMAVDRPIVGVNHLEGHIYSSWLYASGSAQTPEPNFPLVALLVSGGHTELILMRGHL
                     EYQRLGGTVDDAAGEAFDKVARLLDLPYPGGPSIQKAAQEGNPRAFNFPKARLSEPFN
                     FSFSGVKTAVLRTVQDLKAQGKSLPTADLAASFQAAVVSALFEKTISAAQQFDAKEII
                     VAGGVSANKPLREAFRSQTLFKVHIPHISLCTDNAAMIAAAGYQHLARGERDNLDFDV
                     LPTWYLTPTA"
     gene            complement(6190..7428)
                     /locus_tag="PKL60_01295"
     CDS             complement(6190..7428)
                     /locus_tag="PKL60_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNW94799.1"
                     /translation="MKAQIDALMQQNGADALWISGAGQHNSAMVYFTGIAHLTSADLF
                     VIPGRTPVLCHGPMERDEAAKSGFQLISYADYDLTALIKETNGDLGLASALRYKQILE
                     SIGLTKGKVLLYGVRDIGPFFAVIQQLQKLLPELELTGDGNDAILLEARATKDERELE
                     HIRAMAQVTTRVVGNTLDLLSSHKVHGETLVKADGSPLTVGDVKTQINLWLTSYGAEN
                     PEDTIFAIGRDGGVPHSSGKPTDPIRLGQTIVYDIFPCEQGGGYFYDFTRTWSLGYAT
                     DEVHQIYDQVLQTYNQLASELQVGINPASLQLRACMLLEAYGHETVRTNPRVSSGYVH
                     SLGHGVGLDVHEKPWFGRPKDNSNILQPGSVFTLEPGLYYPEKGYGIRLEDTYYISRD
                     GEFFKFADFPMDLVVPMRAG"
     gene            complement(7425..8792)
                     /gene="selA"
                     /locus_tag="PKL60_01300"
     CDS             complement(7425..8792)
                     /gene="selA"
                     /locus_tag="PKL60_01300"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNW94800.1"
                     /translation="MEALRQIPSVDRLLTHTLTGELIASYGRGWTANALREVLSACRE
                     ELRAGQALPTEEALISRAAALLARQSALFPVQVINATGVIVHTNLGRAPLSQQAQLAL
                     GAAAAGYSNLEYELETGKRGTRNGQVESQLTRLTGAEAALVVNNNAGAVLLALSALAR
                     RKKVAVARSQLVEIGGGFRVPDVLHESGARLLEVGTTNRVHLSDYQSALEQGAQLLLL
                     THHSNFKIIGFASEPGIAELAGLASSAGVPLVFDQGSGALLDTDQYGLPHEMTVQEAL
                     QAGADLVCFSGDKLLGGPQAGIIAGKKAFLDKLRKHALYRALRPDKLCFAALSATLVH
                     YLKGEAQTCIPIHSMLAREESSLREQALGWQQELGEGLVIPGRSTIGGGSLPTETLPT
                     WLLALDARSPQKFLARLRACQPAVIARVENKRVVFDPRTILPDEDHSLLQGIRTALRQ
                     IKDET"
     gene            complement(8796..9209)
                     /locus_tag="PKL60_01305"
     CDS             complement(8796..9209)
                     /locus_tag="PKL60_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94801.1"
                     /translation="MKNPERVLPPFKEFLPVFLILLAVGGGGLALLLNLTLPTLGPRW
                     LFFFLLVLLGSGAALPAAYFLNLRFPTTPPAERSVIMREALWAGVFVSLLAWLRMGRV
                     LTLPLGVIIAAVFVLVELLLRLWERSRWKPQNPVA"
     regulatory      9304..9400
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            9460..12987
                     /gene="metH"
                     /locus_tag="PKL60_01310"
     CDS             9460..12987
                     /gene="metH"
                     /locus_tag="PKL60_01310"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNW94802.1"
                     /translation="MSPDDAGITIKTAYLDALKERVLVFDGAMGTTLQTMNLTEADFG
                     GKHLVGCNDCLVLNSPATIEKVHTAFLEAGADVIETDTFRANRLTLADYGLEERTLEL
                     NSAAARLARRAADRYSTPEKPRFVAGSIGPSGKLISTNDPQMSDISYDELAEVFREQA
                     SGLIQGGADLLLLETQQDILEVKAAIEGIRRAFAQEKVILPIQAQVTLDINGKMLLGT
                     DISAALAILEGMGVSVIGLNCSTGPEHMRASVQYLSEHATLPISCIPNAGLPMNVNGE
                     AVYPQQPEPFAEMLSEYVREYGVRVVGGCCGTRPEHIRKLAEKIPARLPARKQPEPEA
                     LLASPVQAVPIEQEPVPFLIGERLNAQGSRAFKKLLLAEDYDGMLQIAREQLETGAHG
                     LDVSVAVTERSDEAQLMEKLVKRLSLETPAPLIIDTTEPDVAEAALRTAPGRCLINST
                     NLEAGTEKPLRIFTLAKKYSAAVLALTIDEQGMAKTAERKLEVAQRIYDLAVNTCGLK
                     AQDLVFDDLTFTLATGEEIYRKSAMETLKGLTLIKQALPGVHTSLGVSNVSFGLSPAS
                     RKVVNSVFLHHAIQAGLDMAIVNPAQIRPYGEIPERERELAEDLIFNRHENALPELIA
                     YFDANPEAAAEGKDKGSPLDGLPPEERLAQRVIRRLKAGVEEDIEAVLAENAGTPKGQ
                     TAVKILNEVLLPAMKEVGDRFGAGELILPFVLQSAEVMKKAVAHLEKYLEKKSGESKG
                     KLVLATVYGDVHDIGKNLVKTIVSNNGFEVVDLGKQVPAETIIQAALEQHATAIGLSA
                     LLVSTSKQMPLIVNELHRRKLNFPVLIGGAAINPRFAWRIFKCEDGEYYQPGVFYCKD
                     AFEGLAVMDALTDPQRYQASLEDLRRKAEHEYAAAQTRPERAARTSTVRSVAPAENIP
                     EPTQWGVRVVKEMPLPLVARHLNLNELYRLSWGAKNAHGSEWKRLQAEFDARREAMLR
                     SAEKEGWLKPQGVYGYWPALADGDTLVIYDPQSLMDAQPRELERFEFPRQIGGENLCL
                     ADYFLPLGSARFDAAAFQVVTVGDAAARRFAELEAAHDYSEAYFTHGLAVQMAEAAAD
                     FLHAHIRRELGLKDDQGLRYSWGYPAIPELADHSKLFRLLPAGSALGMQLTSAYQLVP
                     EQSTAAIIVHHPLAKYFNIGVNRVEQLLG"
     gene            13513..14703
                     /locus_tag="PKL60_01315"
     CDS             13513..14703
                     /locus_tag="PKL60_01315"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNW94803.1"
                     /translation="MRAKKIIVPGLLLTAVLLISGCTLSAPRELTDLEETAVVQTLTA
                     MAPTLPPPTETPTPTATSTSSPTPTKVTSIGPDNFPTDVNPLTGLQVEDPGLLDRRPV
                     IVKVANYPAVGRPHAGLSYADIVWEYYIGEGSNRFAALYYGQDGTKIGPVRSGRLVDP
                     ELARMYQAVLAFSGANEENVLPRIYDLLGSRILTEGNCPGLCRDDYTVIGVFGNSADI
                     TAQMAARGVDESKPDLTGMLFDTRLPAGGKKAEKTTLFYNYLDKGDWVYDETSGKYLR
                     WIENANNYDQMIPLVDRLTNEQLAFSNVIMAYATYTEYSSVLHDIAVWDNTSGMKAVV
                     FRDGQAFEVTWKATNQEKPIQFFDAQGNLFPLKPGNTWMNLIGSSSSLEVTNAEWLFT
                     FRMP"
     gene            14708..15433
                     /locus_tag="PKL60_01320"
     CDS             14708..15433
                     /locus_tag="PKL60_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNW94804.1"
                     /translation="MRTLVISDIHSNADALKAVIADAGAFERVWCLGDVVGYGPDPNE
                     CIQILKDLPGLVCILGNHDAAVMGFIDLDAFNDEASAAVRIQERMITQESREFLELLD
                     VTRVEDGVALAHGSPRNPIWEYIMNARTAKENFRAFDTQVCLIGHSHIPVLFIETGAR
                     NPDILLPSSGDMWRSEKRFLLNPGSVGQPRDHDPRAAYVIWDDEEEIWSYHRAAYDFK
                     PVQERILQLGIPSRHAYRLAEGI"
     gene            15502..16440
                     /locus_tag="PKL60_01325"
     CDS             15502..16440
                     /locus_tag="PKL60_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNW94805.1"
                     /translation="MKKRWIFLSFLLVFVLTACASRATEQAAEAPALAVPTEAPAQAS
                     KDAYNDSSGSQGVASSEEASERLVIKQASMRVSVADPSQAAQSVMSMAEAMGGYVVNS
                     NQWNSTDSSGKAYTQTSIMIRVPAERLDEAMQKIREMAADAKNGVLSESVNGQDVTSE
                     YVDSQSRLRNLEAAEQQLVQLLDSATDLEYTLEIFRELTKIRSDIEVLQGRIKYLEES
                     AALSSVTVEFVAEASLQPIQIGPWKPTGEAKKAVQSLIRSAQKVGTGLIWFVITWVPF
                     LLPIGLIIYLIVRGSKKRKAARKAAAEESAQPQAKE"
     gene            complement(16718..17341)
                     /locus_tag="PKL60_01330"
     CDS             complement(16718..17341)
                     /locus_tag="PKL60_01330"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNW94806.1"
                     /translation="MLTRILPTGDPQALEAALQTLRSGGLVVFPTDTLYGLGCDPTNP
                     NALQAIYAAKNRPTLKAIPVLIGESSQLSGLVTALPPQAERLIHRYWPGPLTLILPKN
                     PSLPPDLTPYPGLAVRMPDHPFARTLLKAFGPLAVTSANISERENPQDAGEVRAQLDG
                     RVDIILDGGRLRGGTASTIIDGTGAELKLLREGPIAFADVLALWAAE"
     gene            complement(17354..18211)
                     /gene="prmC"
                     /locus_tag="PKL60_01335"
     CDS             complement(17354..18211)
                     /gene="prmC"
                     /locus_tag="PKL60_01335"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNW94807.1"
                     /translation="MKLPTDANLAELQHAIRTQLPPDLPDASAGILVLIAHVLQQPKT
                     WVLAHPEARLNTQARQQLSQLAQRFAQGEPLPYLTGTQEFFGLTFKVSSAVLIPRPET
                     ELLVEEALTWLQLLDRPVRLLDVGTGSGCIALTLANRVLSLQGTASDNSAEALEIARA
                     NARALRVSERVTFLQADLIPENFPKYDLICANLPYIPTRKLGTVNSIAFEPETALDGG
                     EDGLRLLRPFFSLSAPHLQNPGLLLAETEAELGAETLALAQENFPEARIDLLKDLNQR
                     DRLVRVQLA"
     gene            complement(18195..19277)
                     /gene="prfA"
                     /locus_tag="PKL60_01340"
     CDS             complement(18195..19277)
                     /gene="prfA"
                     /locus_tag="PKL60_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003717655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNW94808.1"
                     /translation="METQLEEKLEHILGHFEELEQKLAEAGSDYQRVAELSKERAELE
                     PVVNLARQYKAAQSRQEETRHLLASAEGEMRELAELELQDLSEQLDTLQTELRSLLVP
                     KDPRDNRNVIMEIRAGTGGDEAALFAADLLRMYLRYADLHHYKTEILSANEIGIGGYK
                     EVTFAVKGKGAYSRLKFESGVHRVQRVPETESQGRIHTSTVTVAVLAEVEDVEIDIPD
                     ADIQVDVYRSAGAGGQNVQKNMTAVRITHLPTGIVVACQDERSQLQNRLRAMGVLRAR
                     LYEIEQQKRQSELDATRRSQIGTGERSEKIRTYNYPQSRVTDHRINLSSFNLAGVMSG
                     TELDTFIDELQVADEADRLANYETSD"
     gene            19663..20412
                     /locus_tag="PKL60_01345"
     CDS             19663..20412
                     /locus_tag="PKL60_01345"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNW94809.1"
                     /translation="MELYFIRHGQSENNALYGEEDYDIDDRRSDPKLTRLGNKQAQLA
                     AECFLRSAASDPIKRNGWQNHAECGLTHLYCSLMERAVQTGSIIAQTLNVPLYGVPDL
                     HEVGGIYYDDIVDGVIRIRIEHGLTPAYLAQTYPMLTLLEPIPAEGWWRGGREERAAR
                     IPRAVRMLDLLFARHGNTQDRVGVITHGGFFACLFRAIFRLDLDEPNQHLLPYQLELN
                     NCGITRFSIEEARYLLVYHNRTDFLPDDLVT"
     gene            20540..21862
                     /locus_tag="PKL60_01350"
     CDS             20540..21862
                     /locus_tag="PKL60_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNW94810.1"
                     /translation="MPVYVNIIIIAVLFYFNGIFAMYEIAMVSARKTRLQQRSEDGNR
                     GANDALELLKDPNQQYLSTIQIMITMIDTLAGGIGGAMLAEPVADLLRKISWLAAGAD
                     TIALILVVVVITYFSIVLGELIPKRLAVSKPEDVVCRLSPGIRTMTRIFRPLTQLLSA
                     STNFGIKLLRIDTQAEPSITEDEIKVFIEQGRDIGLIEQTESDMFSGVFRLGDRRVDA
                     LMTPRTDLEWIDIEDDQNTIIHALSNSEYSLLPVARGSLDDVLGMVNIKELVGVDLTK
                     PGFRLEDYIREPMFVPENMQALKVFEQFRATGTHQALVIDEYGGVQGMVTLYDVLEAI
                     VGEIPLDAEDLEQEIVQRSDGSFLLDGMLPVDEIKELLDLDELPEEERAGYQTMSGFV
                     MNQLGSIPKIGQAFTWENYRFEVVDMDGRRVDRVLVTVLPGASETSSS"
     gene            complement(21870..22766)
                     /locus_tag="PKL60_01355"
     CDS             complement(21870..22766)
                     /locus_tag="PKL60_01355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW94811.1"
                     /translation="MPTPNFSLPEIKLLASENWREYALLDSGEGKTLERFGPYLLISP
                     DPQAVWKKSLPAAEWQKAHAVLREENKEYGGSWQLLKPIEKTWEMHYQSLTFQLQLGT
                     SRHIGVFPEQASHWDWIMERIQSEKRPLRVLNLFGYSGLATLAAAKAGAEVTHVDSSK
                     HALNWASYNLKLSGLADKPVRWIAEDALKFTQREARRGNKYDGLILDPPKFGRGSQGQ
                     VWDFFKKLPELVEACRAILSPKASFIAFTTYAISASALVALQAVQDMTHNQGKYECGE
                     LVSVEESAGHLLSHALYTRWTP"
     gene            complement(22776..>23698)
                     /locus_tag="PKL60_01360"
     CDS             complement(22776..>23698)
                     /locus_tag="PKL60_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNW94812.1"
                     /translation="AARPLWAGLNWYTHGMTKRVQAFLQLVRPKDYFFFVLATSLLGI
                     AAAQGEFGLRFVLVCAANFFASSFTFIFNQLADAPADTFRQKKSAPNPIAQGSISASE
                     AALTALAAAVLSLALFWLLDWRLLFVGLVLLGFGLLYSERGTRVHGISMLDLAGHSWL
                     LAPALSLSGYLAFQPRFTTELLFLFLTVIFLSLLGEITHERSSLKALKEAPARQTVLN
                     ISARAVHFLVIACASAALLSGIILFFFLRVLPLRAAIFVLVCGGILLIPGLFRSNREP
                     TRLPLHIPLEKAVALGLLVEFLTSFLIRWL"
     assembly_gap    23699..23798
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23918..25069
                     /locus_tag="PKL60_01365"
     CDS             23918..25069
                     /locus_tag="PKL60_01365"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HNW94813.1"
                     /translation="MDFDRIIERRHTESIKWKYFPEDVLPLWVADMDFLSPPEVIEAL
                     HQRVDHGIFGYGIPPQDLSEIVVNYISRHFHWAPLPSQVSYIPGVVTGFNLAIRAFCE
                     PGTSVLFHTPAYPPFFGAPKSAKLPFVTNSLLRGTDGAYSIDFERFEEQIIAERVSVF
                     ILCNPQNPTGRVFTREELTRLAEICLRHKVIIISDEIHCDIVYDGRQHIPIASLDAEV
                     ANSTLTLMAPSKTYNIAGLYASVAVTSSPELARKFSEVKNGLISGPDLLALTAARAAY
                     LYGDGWLQGVLGYLQANRDWLSEAIARDFPGMRMCKPEGTFLAWLDCRGLNLPLEPQK
                     FFQDRARVGLNSGADYGSEGLGFVRLNFGTQRAVLQEALERMTNALNSL"
     gene            complement(25138..25353)
                     /locus_tag="PKL60_01370"
     CDS             complement(25138..25353)
                     /locus_tag="PKL60_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNW94814.1"
                     /translation="MSERLVGTIKWFSAPRGYGFIGQETGEDVFVHFSAIEMEGYKRL
                     REGQRVEFSIEDGPKGRQAANVVVLDK"
     gene            25799..26605
                     /locus_tag="PKL60_01375"
     CDS             25799..26605
                     /locus_tag="PKL60_01375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW94815.1"
                     /translation="MTINDPRYLKKYQYRDPGNLNSRITIHTRYSTARQSWADFIFEQ
                     LQPLAGERILELGCGPAAQWQTTFSRLPEGARLILSDLSLGMVKEARHVLATAGRAAF
                     LIQEAQTLAFSEQGFDVVMANHMLYHVPSPARAIQEAARVLRRGGRLIAATNGARHMA
                     ELDVLLREFEPRFETETAMSRPFSLENGREQLEQYFGSVELIPYVSDLWVTDAEPLAE
                     YVFSTPTGQIWFCKDQIGDMTAFFQRKIDQNGGIYIHKSIGVFRASHPKA"
     gene            complement(26633..27454)
                     /gene="uppP"
                     /locus_tag="PKL60_01380"
     CDS             complement(26633..27454)
                     /gene="uppP"
                     /locus_tag="PKL60_01380"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HNW94816.1"
                     /translation="MTVIQALILGIVQGLTEFLPVSSSAHLVLVPFLLNWKLDPTQAF
                     VFDVLVQLGTLAAVIAYFWKDLLAILKAWAAGIRARQPFAEADSRLGWLVILATIPAG
                     LAGLLLKDKVEAAFSDPMLTAVMLFITAVLLTLSEVFSKKTRETAQLSWLDSFIIGVF
                     QAVAIFPGISRSGATISGALFRGLKRQDAARFSFLLAIPIMTAAGVLALKDLLAVPGL
                     AAFLPVVLLGGIVAGLVGYFVIRWFLTFLKTRSMLPFALYCALVAVAVLLVMGIR"
     gene            complement(27451..28569)
                     /gene="tgt"
                     /locus_tag="PKL60_01385"
     CDS             complement(27451..28569)
                     /gene="tgt"
                     /locus_tag="PKL60_01385"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNW94817.1"
                     /translation="MTPFTWTLDGTDGNARAGTFTTPHGALNTPVFAPVGTQATVKAL
                     TPAQVEELGAQMVLANTYHLFLRPGDELVAEMGGLHRFMQWNRPILTDSGGYQVFSLT
                     RTRKIDEDGVTFKSHIDGSLKRLSPEISMRIQENLGADIIMAFDECAVPTDYDYSRLA
                     MRRTHAWARRCLEAKRRDDQALFGIVQGGVFTDLRAESAREISTLGFPGYAIGGLSVG
                     ESKTEMYRTITEVNSILPADKPRYLMGVGTPEDLIEGVRRGVDIFDCVLPTRLARHNS
                     AMTMRGRLNLMNAGFARDERPIDPDCGCYTCRHFSRAYLRHLVNAKEMLAATLLSIHN
                     LHTLVQLSRDMRSAILAENFEQFAREKLAQLNTKETEK"
     gene            complement(28566..29207)
                     /locus_tag="PKL60_01390"
     CDS             complement(28566..29207)
                     /locus_tag="PKL60_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94818.1"
                     /translation="MENEVLNRLSSQRGKRGDEENFRLAEAIASSMDAEAVRELVTAL
                     PKAPAALSSDILKTLYETGKRQPALISPYAEVFLKLLRAKNNRLVWGGMIALESIAAI
                     RADELFPQVPLFQESVQTGSVITRDAGIGALSGIAAANPQYALAIMPFLLDHLRTCRP
                     ASVAQHAEKISACVSPAYAGTFAEVLQARLPDLSEPQARRVNKLLKNITKEAK"
     gene            29749..30858
                     /gene="alr"
                     /locus_tag="PKL60_01395"
     CDS             29749..30858
                     /gene="alr"
                     /locus_tag="PKL60_01395"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNW94819.1"
                     /translation="MDINSYSTWLEIDLNAIRNNVARIKDLTKVEVMAVIKANGYGHG
                     ARPAAQAAVQGGATWCGVARIEEALALRRAGISCKLLVLGYTPPSKIPEAIDQQIVVS
                     LFDPDLTRSYLEFARINGGILKAHVKVETGMGRLGMLPEDVPDFLRELRGGPISVEGI
                     FTHFAQADEPQSDYTYKQIRIFNDLLARLESSELKPQVVHAANSAAVFNFPEAWYDLV
                     RPGDAIFGMAPSALTPLGSAFKPALTWKARLISVRGYPAGSGISYGSQYVTTGNERIG
                     VMPIGYGDGFRRIDNQEVLVGGIRVRVVGRVCMDQCMLQLDKVPDAKAGDEVVLIGTQ
                     AGETISVDEVAGRWSTINYEVVCGLADRLPRIYLE"
     gene            30855..31523
                     /locus_tag="PKL60_01400"
     CDS             30855..31523
                     /locus_tag="PKL60_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNW94820.1"
                     /translation="MNASFLSAEFHCHTRFSPDSLMRIEALLQACRQKGIDKVAVTDH
                     NRLAGALKAHEMAPDQVIVGEEIQTTQGEILAYFLSEEIPAGLEPAEVVRRLRAQNAF
                     ISVAHPFDPYRGSRWEKGTLESLVPLLDGIEVFNARCVESAFNQQARAFAREFGIPEM
                     VGSDAHSVFELGRAVLQLPEFHTADELRTALRSSQQKTRLSGSYVHLISTWAKLVKRV
                     GNTR"
     gene            complement(31569..33209)
                     /locus_tag="PKL60_01405"
     CDS             complement(31569..33209)
                     /locus_tag="PKL60_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNW94821.1"
                     /translation="MKSFRKHAFTISLLLSLLSFFALLLPQTAAARAVNPQGIGPEAA
                     NPTEDLSSAKVHGYYFYSNTCSHCLDILTNILEPLQAENPGQIDMRLLELGNPEYYRA
                     LLAVEEAYQVKPEARGLPTVVLGGQIFLGEAQNKEHLAEAIQAGIAGSGIAFPEIPGV
                     DPKLMLSVTPANTAGELEACTTSNPEACQVDTPIYAAYFYQVGCKECNRADADINYLR
                     GLYPQLVVEELNIYDNTPLANWMAERAGRSADLKVPALFIGDQAWIGEGEITTQNLEP
                     VLQVLSASGSPRFWDAFDEKQAAGALNERFQSAGWVAVLLAGLVDGLNPCAFATIVFF
                     ISYLTLSGKKGKEVIFTGISFTFGVFLTYLLVGLGFYKVLDLIKGALAVISTVVYALT
                     ALLCLVLAALSIKDFFTARKGELEDMALNLPKALRKRINATVHGTKKIGSYALGAFVS
                     GALISLLELACTGQIYLPTIIFMSSVPELRGKAIGYLLLYNLMFIIPLIIVFILAYYG
                     TTSKQLSAFLKKHAAAVKLGMAAVFLTLAAWLILTLLN"
     gene            complement(33373..35226)
                     /locus_tag="PKL60_01410"
     CDS             complement(33373..35226)
                     /locus_tag="PKL60_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNW94822.1"
                     /translation="MVEIENITEIRPRVLGEFHVEQFVPRTEAIARAWEWGKQVHGGQ
                     LRLSGEPYFETHCGWVAGFVDNLVHKESWTIAALLHDTIEDTGESLDKIKSLFPADLG
                     EEIAHIIDGVTKMSNPRDGSTRELETLRKIAMFRDPAVFVVKLADKSHNLMTLQFMSA
                     AKQCQKASEAIRSYGKLAGILNCYRWRRWIEDMAFPYAEPESYDTVKTKIDRDPRLNL
                     NFIRYYLGELGRLMDAESLDGAVRFTVNGYWQSWDKLQRMARARRTSLDDFSAVNDIV
                     SFRMIVRDNDEAACYRLLSRVNRYFSKNLDQDRFDDYIASPQNGYRALQVTTWQPGVG
                     AVEVAIATEEMEGENTWGVIYAVNNNKDISKYRPVQILTPYGGTRFLQEGGTVLDGVA
                     AIQEFYLDKISKVLVNGQERHLYDTLSPGDVVEVISTGPHQVPKPEWLDHCDVSTARR
                     LRIVLARVALKEASQKSKALIHPLLAKRGILALEDVSALAHNRVEQLLGLMACASLDD
                     LYSAVGGGSISLAQLENALDLVGISRVGLGWITVEISGSNTANRPGALAYFASLVSEA
                     DGNIIRTVHTTAEDGSFYLRLLLTGISEEGKQKLSESFARSRFPLDRVEIV"
     gene            complement(35326..35571)
                     /locus_tag="PKL60_01415"
     CDS             complement(35326..35571)
                     /locus_tag="PKL60_01415"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HNW94823.1"
                     /translation="MIYPLLALSILTFLLFGYDKNAARRNQWRIPEKVLLGLSILGGA
                     AGGLLGMLTFHHKTRKNYFWVILITSACMHLFLLTTV"
BASE COUNT         7670 a         9836 c        10521 g         7502 t
ORIGIN      
        1 gttccacacg acctgcggcc atgttttgtc gggttgcgct gccgttccac ccgacctaca
       61 accatgtttt gtcgggttgc gctgccgctg cacccgacct acgaccatgt tttgtcgggt
      121 tgcgctgccg ttccacccga cctacgacca tgttctgtcg ggttgcgctg ccgctacacc
      181 cgacctacga ctgccgctcc acacgacctg tgaccatgtt ttgtcgggat gcgctgccgc
      241 tgcacccgac ctacgactgc cgctgcaccc gacttacaat ctgttatcct gcctgcccgt
      301 ggaacggtca ctgcgagacc ctttgccagg aaaacttagt cccgcatcgt gagatcaagc
      361 ggcaaagggt cgtggcagtc tgccttaaat aatgcaagta ggagcgcgac taaatcagct
      421 caaagacaga ccagtttttt atgcgtccaa tgcgctgaac gaaaggaaaa gaccgattac
      481 cacgctgttt tgccttggaa tccaaataga aaaaatgcag cggcaaaaca gctggcaacg
      541 acaaacctaa ctgccaatgc acaccctact ctctgtgact gtaatttcgc ctgaaaagcg
      601 agcagagatg catgcgcatg tagcgggaaa gcagctcgtg aagaggtatt cactgcccac
      661 cgaggaactc atcattagcc atgaaaaaaa accggccgcc aatttccctg gcagcctgtg
      721 ttatctctaa attaaaacct gtcaaagccc aaaatcgtgc gggtttaccg gtgaaccatc
      781 gtagcggatt tcaaaatgta agtgcggtcc ggtggattgg ccggtggagc ccatgtagcc
      841 gatgagttgg cccgcgtaga cctccacgcc gcagccctgg atcataccgc cgtccatcag
      901 gtgcgcgtac agcgagaggt agccgttgcc gtggtcaatc acaatcgtat tcccgtagcc
      961 gcgcgtggac catccggaat agataatcac gcctgagtca gcggcataaa tctcgtaacc
     1021 catccgcccg gcataatcga gcccgttatg cgtgggtggg gtgtactcat agccggaaat
     1081 ccaatgctct gaggtcggga actgccagct gccggtaccg accgcccagg cagtgctgtt
     1141 acaggcgaaa gcaccaaggt acgaaacgtt tggatgctca ccaacgccgt cctcgcccgg
     1201 acccaccggg gtgacccagg taaccggcgg acgcgtgccg cccggaacgt agagcagtgt
     1261 gcctgctttg atgttggggt ggctaaggtc cgcaaacgcg ctgcggtcaa ggtgattcag
     1321 cggttcgtcc agaatcacat cccggctgac attgtaaaaa gacgcgacct tgtccaggtt
     1381 ttccttataa ctccagccgt ggtaaacgcc gttacctggc aggatgaaaa gcttttgacc
     1441 tgggacgatg ccgtctgggg tgtcccccag caggtagcgg tttgcccaca gcacagtttc
     1501 gggcgtaagg ccaaatttgt ctgcgatggt gaagagggtg tcttcttcct gcaccacgta
     1561 ggcaatgatt tccttgcgcg gggcagggta agtgtcgggg tccccgacgg ggattttttc
     1621 gatttgggtg gcagccgcgt aggtaagcag gctgtccgcc gagaccgttt ccgggcgggt
     1681 gccgcctggg acgaccaggc gtgttccggg cgtaatattt ggcagggaga gattgcccaa
     1741 agtcgcgcgg tcgagtttgt tgcccgggta gtcaataatc gcgtcaggtg taaccccgta
     1801 aaaatccgag acgccgttca gcccttcgcc ggcctgccag atgtggtaaa cgccgtccac
     1861 cggcaggatg taaatcgaga cgccgggcag gtaaaccgcc gggtcgtcgc ccagttcgta
     1921 gcggtttgac cagagaatcg tttccggcgc tatattgaat tttgaagcaa tcgagaaaat
     1981 cgagtcgcct tcctgaatcg tgtaggtgat tacctcggtg cgtgcctgtt cctcggtcgg
     2041 cagaacagct gccgtggcgg ttgctgtggg ttctgcagtc tcctcttcaa gcgccagctg
     2101 cggcgcgttc agcgccgtgg cagtgggcgt gggcgcggcg cttttatccc cgggctcgct
     2161 ggtcagccag gcgctgagga taatcagggc tgctaaaatc aagaggagcg cggcgatact
     2221 tccgggctgt gtgtgccagc ctttgcgctg tgggcgcggt tggtggctgc cttcggtttg
     2281 cgcggcagcg ggggtttcag attcagacag cggtgcttct tccgctttca gattgtgggg
     2341 ttggacattc attggaactc caaacaggca taataaccag cttctttttc cttgtcaagt
     2401 tgtgttacgg cataggctgg cgggctccaa aaccggtaat ttcattaccg gatttcccgg
     2461 agcaaggtct ggcggatttt atcaaattgc ggcggtcttt caaggcatag attcccgggg
     2521 ttgcggtcat acgcgagcag gttcgttgcc tgatagcctg gacacgaagc aaggctggcg
     2581 tgctgagagc agtcctgtct actcgtggaa gtacctggcg agcagtgggt tcaggtcttc
     2641 ctggctgtgc ctggcaattg ccgcgaagaa atcctcgcgc gtggcgatgc ggtaagtgtt
     2701 ggtgagggcg taatccttga gggcggcaaa gaaggcttta tcgccaatca ggtcgcgcag
     2761 ctcttgcagg aactcggcgc cgcgcaggta gaccgcgtcc cggtaagccg ggtagccgcc
     2821 ggaaaggtaa atatccgaat tgacatagcc cttgggcgtg aagccgtcca cacggttatt
     2881 ccaccaccac tgcacgaggt ccgggtggta gcgctggtaa aaaagcacct catggtaagt
     2941 cgccagggcc tcgtccagcc agggttcttc cgcctggtcg ctgccgacca tgctgtagaa
     3001 ccattgatgc gagatttcgt gcggggtcag gatggtcagg ttggtcttgg gcgttttgtt
     3061 atagaaattg aacactccat aactgatcat caccatcccg tccatttcca tattgtgcag
     3121 gaaatccgcc gcgacgatgg aaatcatgtc gcgcggatag ggatagtaga gctcgccgaa
     3181 aaatcgaatg gcttgcgcgg cgatttcggt gacggctttg ccagcttcct gcgcttccat
     3241 gaagacgtac gaatgaatgc gcacgccgtc ctggataatt tcatgctcaa aatagctgtc
     3301 gctaaccgag aacgaaaaac cccgcgccag ctccaaatgg taataccgca agccgtcttt
     3361 ctctgtggcg ggagcgctgg cggcgacttc aatcagctcc tgccggtcgg tcaaccgcag
     3421 actgacgtca aaatccgcca tctctgccac cacatactca cccacctgca tattgttctc
     3481 cagctgctgc gggtggataa tccagcctgt gccctcatcg taggggggga tatacggata
     3541 ccagttggtc agattcgtct gccggcccga gacgccgtaa gtgccctcgt gcgcaggcag
     3601 gttaagctgg tagctgaatt tcaggctggt ggtttgcccg ggcgcgagcg gggcaagcag
     3661 cggaactgta aagcgcacgc cgtcttcctg ccacccgcta atcgggctgc cgctgccgtc
     3721 cgtgagaccg cgcagagaga aactgcccgg atagtacttg ggcggtacga ccactgccaa
     3781 ttccgagaag gtctgactgc tgcggttggt gtaagtgatg atttcgtcca caacagcgta
     3841 ttgcgtgtag tagttgagta tcaggtccaa ctggtaatgc gtacgatttt cgggttctgg
     3901 gatggccgcg cccggattcg acggggtgga aggggtgact tccaccagcg tggtcgcaga
     3961 ttctgcttcg gtcggggcag cctggacggc aggcggcgcc tcctcagaca gctgcacctg
     4021 tggggctggc agcgtgctgg tcgggccggc agtcgttacg ttcgcgcagg cgcccagaat
     4081 gagcgcaata gaaagcagga ggaataaccc tggggatttc ttcaaatgga cctcacaacc
     4141 tgaccgattc taccagaaaa acaagcggaa cacttccggt cggcgcggaa gcgaggatat
     4201 cagtgacaaa gctgctctga ccggtgttca ggcacgtatt tcgccaaaga ggaactgagc
     4261 tctgccgcgt caggaaccca gtccgaatgg tttttcaagg ttcccgctga ttaaagcggc
     4321 ggctgcgtgg gcgcggtgtt tctccctttg gggaattcca aaaatttatg cttcggcttc
     4381 gataatcttg atttcacggg tcagttccag cacctggcgg aacatggcga tgcaggggca
     4441 gtacttttcc tccgagagct gcaccgcgcg ctccacatcc tcaggcttga tgccgtgccc
     4501 gctgacggta taaataagat gcatgcgggt gaagacctta gggtaggttt cagctttggc
     4561 gaccacagcg cgcacctcaa aagcggtcac atccgcgcgt ttctttttca ggatggaaat
     4621 gacatccatg gcggtgcagc ctgccgtccc gattgccagc agttccgttg gtctgaagcc
     4681 atcatcatgc ccgcctgcta cttcgttcga atccatctta aggctgaagc cggtgtcgga
     4741 gctgcctgta aaactcatat ctccttgcca gactgctttc gcttccattt ttctccttat
     4801 tgttcaacca actggtcgaa ccaagcttat ggttatcagg taaacgcgcc tgtttgcgtt
     4861 ttcccgcagt tcccgcacac caggttaagc tggctgtcgt aatccacata tccgacctgg
     4921 cagaatggac agggcagtcc gtaacggagg gaaaaggggc cgctggagcc gcacacgtgc
     4981 aaggcttttt ctgcccttcc actctcaggg gaggtaaagg actgcgcagg aagttcctcg
     5041 ttggtttcgg gcgggctgct cactttcatc tccagggtga taccttcctc atgcttcttt
     5101 cagggttcgc tcccgggcct ggcgtacagc gcttccagca ccactcgcgc gggtcatgcc
     5161 gtcggggtca ggtaccatgt tggcaagacg tcaaagtcca ggttgtcgcg ttcgccgcgc
     5221 gccaggtgtt ggtaacccgc ggcggcaatc atggcggcgt tatctgtgca caaactgata
     5281 tgcgggatgt gcaccttgaa gagggtttgc gagcggaagg cttcgcgcaa gggtttatta
     5341 gccgaaaccc cgcccgcgac gataatttct ttcgcgtcga attgctgcgc cgcgctgatg
     5401 gttttctcaa ataaagcgga aacgaccgca gcctgaaaac tcgctgccag gtcggccgtc
     5461 ggaaggcttt tgccctgggc tttcaaatcc tgcacggtgc gcagtactgc cgtcttcacg
     5521 ccgctgaaag aaaagttgaa gggttcggaa aggcgggctt tagggaagtt aaaggcgcgc
     5581 gggttgcctt cctgcgcggc tttttggatg gaaggaccgc ccgggtaggg caggtccagc
     5641 aggcgggcga ctttgtcgaa ggcttcgccg gcagcgtcgt ccacggttcc tcccagacgc
     5701 tggtattcca ggtgcccgcg catcaggata agttcggtgt gcccgccgga gacgagcaaa
     5761 gccaccagtg ggaaatttgg ttcaggcgtt tgggcgctgc cgctcgcgta cagccaagag
     5821 gagtaaatat gcccttccag gtgattcacg cccacgatgg ggcggtcgac cgccatcgcg
     5881 agcgctttgg ccatgttcgc gccgaccacg agcgagcctg ccaggccggg cccgcgcgtt
     5941 gcggcaatgc cgtccacatc gctcagctcc aggtgtgcct gctgcagggc ttcatttact
     6001 gtcgcgtaaa cggctttgat gtgttcgcgc gaggcaattt ccgggtagac cccgccaaac
     6061 tgggcgtgca ggtccacctg gctggcgatg atggaactga gaatctcgcg gccatcctct
     6121 acgactgccg cggcggtctc gtcgcagctg gattcgacag ctaaaacgcg cagttgcgga
     6181 gctttcatct tatcctgctc tcatcgggac gaccaaatcc atcgggaagt ccgcgaattt
     6241 gaaaaactcc ccgtcccggc tgatgtaata ggtatcctca agccgaatac cgtaaccctt
     6301 ttcaggatag tataacccag gttcgagcgt gaagacagac ccgggttgga ggatgttgct
     6361 gttgtctttc gggcggccaa accagggctt ctcgtgcaca tccaggccca cgccgtgccc
     6421 gagggagtgc acatagcctg agctgacgcg cgggttggtt cgcacggttt cgtgcccgta
     6481 ggcctccagc agcatgcacg cgcgcagctg caggctggcg gggttaatcc cgacctgcag
     6541 ctcggaggcg agctgattgt aggtctgcaa aacctggtcg taaatctggt gcacctcgtc
     6601 cgtagcgtaa ccaaggctcc aggtgcgcgt aaaatcgtag aagtaaccgc cgccttgttc
     6661 gcaggggaag atgtcgtaca caatcgtctg acccagccgg atggggtcgg taggcttgcc
     6721 cgagctgtgc ggcacgccgc cgtccctgcc gatggcaaag atggtgtctt ccgggttttc
     6781 tgcgccgtag ctcgtgagcc ataaattaat ttgggtcttc acatccccaa cggtcagagg
     6841 ggaaccgtcg gcttttacca gcgtttcccc gtggacctta tgcgagctga gcaaatcgag
     6901 cgtgttgccg accacgcgcg tggtcacctg cgccatggcg cggatgtgtt ccagttcgcg
     6961 ttcgtccttg gtcgcgcggg cttcgaggag gatggcgtcg tttccatcgc ccgttagttc
     7021 caattcgggc agcagttttt gaagctgttg gattacagcg aagaacggtc caatatcgcg
     7081 cacaccatag agcaggactt tgcctttggt caggccaata ctctccagaa tttgcttata
     7141 gcgcagggcg gaagccaacc caaggtcccc gttagtctcc ttgatgagcg cggtcaggtc
     7201 gtaatcggcg tagctgatga gctgaaaacc tgatttggcg gcttcatcgc gttccatcgg
     7261 tccgtggcac agaacaggcg tccggcccgg gatgacgaac aagtccgcgc tggtcaggtg
     7321 cgcgatgccg gtgaagtaca ccatggcgga gttatgctgc cctgcgccgc taatccagag
     7381 ggcgtccgcc ccgttttgct gcattaatgc gtcaatttgt gctttcatgt ttcgtccttt
     7441 atctgcctga gggctgttct gattccctga agcaagctgt ggtcttcatc cggaaggatg
     7501 gtacgcgggt cgaatacgac ccgcttgttt tcaacgcggg caatcactgc cggctggcag
     7561 gcgcgcaagc gcgcgaggaa tttttgcggg gagcgtgcat ccagcgcgag gagccaggtg
     7621 ggcagggttt ccgtgggcag gctgcccccg ccgatggtgg agcggccggg aatgaccaag
     7681 ccttccccga gctcctgctg ccagccaagc gcctgttcgc gtagggagct ttcctcgcgc
     7741 gccagcatac tgtggattgg gatgcaggtt tgggcttcgc ctttcaggta atgcaccagt
     7801 gtggccgaga gcgcggcaaa acatagttta tccggccgca gcgcgcggta gagcgcgtgt
     7861 ttgcgcagtt tgtccaggaa ggcctttttg ccggcaatga ttccagcttg tgggccgccc
     7921 agcaatttat cgcctgaaaa acacaccagg tcagcgccgg cttgcaaggc ttcttgcaca
     7981 gtcatttcat gcggcaggcc gtattggtcg gtatccagta gggcgccgct gccctggtca
     8041 aaaaccagcg gcacgccagc ggacgaagcc agcccggcaa gttccgcgat gccaggctcg
     8101 gacgcgaagc cgatgatttt aaaattcgaa tggtgggtca ggagcagcag ctgcgcgcct
     8161 tgctccagtg ccgactgata atcgctgagg tgcacgcggt tggtcgtccc gacttccagc
     8221 aggcgtgccc cggattcatg cagtacgtcc ggaacgcgaa accctccgcc gatttccacc
     8281 agctgcgaac gggcaaccgc cacctttttg cgccgcgcga gcgcgctgag cgccagcagc
     8341 accgcgccgg cattgttgtt cacaaccagg gccgcttccg cgccggtcag ccgggtgagc
     8401 tggctttcca cctggccgtt acgcgtgccg cgcttgccag tttccagctc gtactccagg
     8461 ttggagtagc cggcggctgc cgcgccgagc gccaattggg cttgctggct caagggagcc
     8521 cggcccaggt tggtatgcac aatcacgccg gtggcattga tgacctgcac cgggaagagc
     8581 gcggactgcc tggccaaaag cgcagcagcg cgggaaatca aggcttcctc tgttggcagg
     8641 gcttgccccg cgcgcagctc ctcacggcat gcggacagaa cttcacgcag agcgttggca
     8701 gtccaccctc taccgtaaga ggcgataagt tccccggtca gcgtatgcgt cagcagacgg
     8761 tctaccgagg gaatttggcg caacgcttcc ataaattatg ctacaggatt ttggggtttc
     8821 caacggctgc gttcccaaag gcgcagcagc agctcgacca gcacgaacac ggcggcaatg
     8881 atgacaccca gcggaagggt gagcacccga cccatgcgca gccaggccag caggctgaca
     8941 aaaacgccgg cccacaaagc ttcgcgcata atcacggaac gctcggcagg gggagtggtg
     9001 ggaaaacgca aattcaggaa gtaggcagcc ggcagcgctg cgccggagcc taagagcacc
     9061 agcaggaaaa aaaacagcca gcgcgggccg agcgtgggca gggtgagatt gagtaacaac
     9121 gccaggccgc ctccccccac cgccagcaga ataaggaaga caggcaggaa ttccttaaaa
     9181 gggggaagca cgcgctcagg atttttcatg ccgaaattat aacgtatttt aaccgcatag
     9241 gcgttacagg ccgcttccca ggaattgacg cgcatccact gctatgttaa acttccacca
     9301 ttcaactcat ccagagaagt ggagggaccg gccctatgaa gcttcggcaa cccgctggtt
     9361 cgggtgccaa ttccggcaga catgttctgg aagatgaggc tggcaagacg ctgaatgcct
     9421 ccattacgag gctttttttg taggagcttg aggtgccgta tgagtccgga tgacgcagga
     9481 atcacgataa aaacagccta cctggatgcg ctaaaagagc gcgtcctggt ctttgacggc
     9541 gcgatgggca ccacgctgca gacgatgaac ctgacggaag cggatttcgg cggcaagcac
     9601 ctggttgggt gcaacgactg tctggtgctc aattcccccg cgacgataga aaaggtgcac
     9661 accgccttcc tggaagccgg cgcggatgtg atcgaaaccg ataccttccg cgccaaccgt
     9721 ctgacgctgg cggattacgg cttggaagaa cggacgctgg aattgaacag cgcggccgcc
     9781 cggctggcgc gccgcgccgc cgaccgctac agcacgcccg agaaaccgcg ttttgtggca
     9841 ggttccattg gcccttccgg caagctcatc tccaccaacg accctcagat gtccgatatc
     9901 agctacgacg aactggcgga ggtgttccgc gagcaagcct ccggcttaat ccagggcggg
     9961 gcggacttgc tcctgctgga aacccagcag gacattctgg aagtcaaagc cgccatcgag
    10021 gggattcggc gcgcttttgc gcaggagaaa gtcattctgc ccatccaggc ccaggtgacg
    10081 ctggatatca acgggaaaat gctgctcggc accgatatca gcgcagcgct agccatcctg
    10141 gaaggtatgg gtgtcagcgt catcgggttg aactgctcta caggtccgga acacatgcgc
    10201 gcttcggtgc agtacctgag cgagcacgct acgctgccga tttcctgcat ccccaacgcc
    10261 gggctgccga tgaacgtgaa tggcgaagca gtttaccccc agcagccgga gccgttcgcc
    10321 gagatgcttt ccgaatatgt gcgcgaatac ggcgtgcggg tggtgggcgg ctgctgcggc
    10381 acccgaccgg aacatatccg taagctggct gagaaaatcc cagccaggct gccggcgcga
    10441 aagcagcccg aacctgaagc cctgctggcc tcgccggtgc aagccgtgcc gattgagcag
    10501 gagccggtgc cctttctgat tggcgagcgt ttgaacgcgc agggctcgcg cgctttcaag
    10561 aagctgcttt tggctgaaga ctacgacggc atgctgcaaa ttgcaaggga gcagctggag
    10621 accggcgcgc acggcctgga tgtgagcgtg gcggttacgg aacgcagtga cgaagcgcaa
    10681 ttgatggaaa aactggtcaa gcgcttgtcg ctggagacgc cagcccccct gattatcgac
    10741 actaccgagc cggacgtggc cgaggcggcg ctgcgcaccg cccccgggcg ctgtttaata
    10801 aactcaacca acctggaagc gggcacggaa aagccgctgc gcatctttac cctggcgaag
    10861 aagtacagcg cggcggtgct ggcgctgacg atagatgaac agggcatggc aaagaccgcc
    10921 gaacgcaagc tggaagtggc gcagagaatt tatgacctgg ccgtcaatac gtgcggtttg
    10981 aaagcgcagg acctggtctt tgatgacctg accttcacgt tggcaaccgg cgaagaaatt
    11041 taccggaaat ccgcgatgga gaccctgaaa gggctgacgc tgataaagca ggcgctgccg
    11101 ggcgtacaca cttcgctggg ggtcagcaat gtttctttcg ggctctcgcc ggcatcgcgt
    11161 aaagtggtca acagcgtctt tctgcaccac gccattcagg ccgggctgga catggcgatt
    11221 gtgaacccgg cacagattcg cccttatggt gaaattcccg agcgggaacg cgagttggcg
    11281 gaggacttaa ttttcaaccg gcacgagaac gcgctgcccg agttgattgc ttattttgac
    11341 gccaacccgg aagcagccgc ggaagggaaa gacaaaggct ccccactgga tggtctgccg
    11401 ccagaggaaa gactcgccca acgcgtcatc cggcgcttga aagccggcgt ggaagaggat
    11461 atcgaggcgg tacttgccga aaacgcggga acccctaaag gtcagaccgc ggtgaaaata
    11521 ctgaacgagg tgctccttcc tgcgatgaaa gaggtcgggg accgttttgg agcgggtgag
    11581 ctcatcctgc cctttgtgct gcagtcggcg gaagtgatga aaaaagccgt ggcgcatctg
    11641 gaaaaatacc tggaaaagaa atccggcgaa agcaagggca agctggtgct cgccacggtg
    11701 tacggggatg tgcacgacat cggcaagaac ctggtcaaga ccattgtttc caacaacggc
    11761 tttgaggtgg tggacctggg caaacaggtg cccgccgaaa ccattattca agccgccctg
    11821 gagcagcatg ccaccgccat cggcctgagc gcgctgctgg tttccaccag caagcaaatg
    11881 ccgctgattg tgaacgagct gcaccgccga aaactgaact tcccggtgct gattggcggc
    11941 gcggcaatca acccgcgttt tgcctggcgc atctttaagt gcgaggacgg cgagtactat
    12001 caaccgggcg tgttctactg caaagacgcc tttgagggcc tggcagtgat ggacgcgctg
    12061 accgacccgc agcgctacca ggccagcctg gaggatttgc gccgcaaagc cgagcatgag
    12121 tacgcagccg cgcaaacccg cccggaaaga gctgcccgga ctagcacggt gcgctccgtc
    12181 gcgccggcgg agaatatccc cgagccgacg cagtggggcg tgcgggtggt aaaagagatg
    12241 ccgctgcctt tggtggccag gcacctgaac ctgaacgaac tctaccgcct ctcctggggc
    12301 gcgaaaaacg cgcatggaag cgaatggaag cgcctgcaag ccgaattcga cgcccgccgc
    12361 gaggctatgc tgcgcagcgc ggaaaaagag ggctggctaa agccgcaagg cgtgtacggc
    12421 tactggccgg cgctggcaga cggcgatacc ctggtgattt acgacccgca gagccttatg
    12481 gatgcgcagc ccagggagct ggagcgcttt gagtttcccc gtcagatagg cggcgagaac
    12541 ctgtgcctcg cggattactt cctgccgctt ggttccgcgc gctttgacgc ggctgccttc
    12601 caggtggtaa ccgtagggga tgcggccgcc cggcgtttcg ccgaacttga ggccgcacac
    12661 gactactccg aggcctattt tacgcatggg ctggcggtgc agatggcaga agctgcggca
    12721 gactttttac acgctcatat ccgcagggaa ctcggactga aggatgatca gggcttgcgc
    12781 tattcctggg gttatccggc catcccggag cttgcggacc acagcaagct gtttcgcctg
    12841 ctgccggccg ggagcgcgct gggaatgcag ttgacttccg cctaccagct tgtcccggag
    12901 caatctacgg cagccatcat cgtgcaccac ccgctggcga aatacttcaa catcggcgtg
    12961 aaccgggtgg agcagctgct tggttaaccg tctggcggcg caagctcgct tgaatggggt
    13021 cgaaagaggc aggcaagtgt cagggctaaa tagaaatgtc ctaatgagtg gatgaaagca
    13081 tcaggacggg tttttcctcg atacgaaccg gtttatttct tcttttcacc attgtttcat
    13141 ttgcgctgac cctgaccctt gcccagcgtc aagcggttct tttttgcctc ggccgagccg
    13201 ctgggggaag gtaaaaacga acttcccgtc ccagcgatgg ttaattcggg aaaaaattgt
    13261 aaatttcgtt cgcagggaag cgtgtaagag gggaaaggct gttaaagcct tatgtgtcag
    13321 ggtatgttcc ttgctttata aaaaaaaact ggaggaatca aatgctgaca ccatagatcc
    13381 gaatcttcat caggacattt ttcttgctgc ttccgaaaat tatgattcct ttgctttcca
    13441 tcgcaagagg tatgcaaagg accggcaggc gcttgttgga gtacaattgg atggctgaaa
    13501 gaaggtgacc caatgagagc taagaaaatc atcgtgccgg gtttgctgct gacagcggtc
    13561 ctgctgattt ccggctgcac cctaagcgct ccccgcgagc tgaccgacct ggaagaaacc
    13621 gccgtcgtac agacgctgac cgccatggcg cctacgctgc ctcctccgac ggaaaccccc
    13681 accccgaccg cgacaagcac ttcatccccc accccgacca aggttacttc catcggaccg
    13741 gataacttcc ccacggatgt gaacccgctg accggtttgc aggtggaaga tcccggtctt
    13801 ctggaccgcc ggccggtcat cgtcaaggtt gccaattacc cggcggttgg aaggccgcat
    13861 gccgggctct cgtacgcgga tattgtctgg gaatactaca tcggtgaggg gtcaaaccgc
    13921 tttgccgcgc tgtattatgg gcaggacggc accaagattg ggccagtgcg ttccggcagg
    13981 ctggtggacc cggagttggc gcgcatgtat caggctgtgc tggctttcag cggcgcgaat
    14041 gaggaaaacg tcttgccgcg catttacgat ctgctgggca gccgcatcct caccgagggc
    14101 aactgtccgg gtttatgccg ggatgattac actgtgatag gggtatttgg caacagcgcg
    14161 gatattaccg cccagatggc ggcacgcgga gtggacgaaa gcaaacctga cctgaccggc
    14221 atgctgttcg ataccaggct gcctgccggc gggaagaaag cggagaaaac taccctgttc
    14281 tacaactacc tcgacaaagg ggattgggtc tacgacgaga ccagcggcaa atacctgcgc
    14341 tggattgaga acgcgaacaa ctacgatcag atgattcctt tggtggaccg gctgactaac
    14401 gagcagctgg cgttttccaa cgtcatcatg gcctacgcga catacaccga atacagcagc
    14461 gtattgcacg acatcgctgt ttgggataac acgagcggca tgaaggcagt cgtgttccgg
    14521 gacggccagg cgtttgaagt tacctggaaa gccaccaacc aggaaaagcc tatccagttc
    14581 tttgacgcgc agggcaatct cttcccgctc aagcccggca acacctggat gaatctgatt
    14641 ggctcaagtt ccagcctgga ggtcaccaac gccgagtggc tctttacctt ccggatgccc
    14701 taaacccatg cgcaccctgg tcatttccga tatccattcc aatgctgacg ccctgaaggc
    14761 cgtcatcgcc gacgcaggcg cttttgagcg cgtctggtgc ctgggggatg tggtcggcta
    14821 tggtccggac ccgaatgagt gcatccagat cctcaaggac ctgcccgggc tggtgtgcat
    14881 cctgggtaac cacgacgcgg cggtgatggg tttcatcgac ctggatgcct tcaatgatga
    14941 agccagcgcg gcggtccgca ttcaggaacg catgatcacg caagaatcgc gcgagtttct
    15001 ggaattgctg gatgtgaccc gcgtggagga cggcgttgcg ctcgcgcacg gcagcccgcg
    15061 caaccctatc tgggaataca tcatgaatgc cagaactgcc aaagaaaatt ttcgggcgtt
    15121 cgacacccag gtctgcctga tcgggcattc ccatatcccg gtgctcttta tcgagaccgg
    15181 cgcgaggaac ccggatattc tgctcccctc ctccggcgac atgtggcgct cggaaaaacg
    15241 ctttctgctc aaccccggct cggttggcca gccacgcgac cacgacccac gggctgcgta
    15301 cgtcatctgg gacgacgagg aagaaatctg gagctatcac cgcgcggctt atgatttcaa
    15361 gcctgtgcag gaacgcattt tacagcttgg gattccgtcc aggcatgcct accgtctggc
    15421 ggaaggcatc tagagatgcg gaacaattcc atatattcct cgtctttaat atagagttcc
    15481 aaacaaacaa ggagatagaa gatgaagaag cgttggattt tcctcagttt tttgcttgtt
    15541 tttgtcctca ctgcttgcgc cagcagggcg accgaacaag cggcggaagc gccggcttta
    15601 gcggttccaa ctgaagcgcc cgcacaagcg agcaaagatg cctataacga ctcaagcggt
    15661 tcccaaggtg tggcctcctc agaggaagca agcgaacggc tggtaatcaa acaagccagt
    15721 atgcgcgttt ctgtcgcgga cccaagccag gcggcgcagt cggtcatgag tatggcggaa
    15781 gcgatgggcg gctatgtcgt caactccaac cagtggaatt ccacggacag cagcggtaag
    15841 gcgtacactc aaacctccat catgattcgg gtgcccgcag agcgtctgga cgaagccatg
    15901 caaaaaatcc gtgagatggc tgcggatgct aaaaatggtg tgctttcgga aagcgtgaac
    15961 gggcaggacg tcacctcaga atatgtggac tcccaatccc ggctgcgcaa cctggaagcc
    16021 gctgaacagc agttggtaca gctgctcgat agcgcgacgg acctggagta cacgctggaa
    16081 attttccgcg aactgaccaa aatccgcagc gatattgaag ttctgcaagg ccgcattaaa
    16141 tatctggaag agtccgctgc cctttcttcc gtcacggtgg agtttgtcgc cgaagcttct
    16201 ctgcagccca ttcagattgg cccctggaag cctactggcg aggcgaagaa agctgtgcag
    16261 tcgctcatcc gcagcgcaca aaaggtgggg accggattaa tctggtttgt gattacctgg
    16321 gtgcccttcc ttctgcctat cgggctgatt atttacttga ttgtcagagg gtcaaaaaag
    16381 cgcaaagcgg cacgcaaggc tgctgccgaa gagagcgcgc agccgcaagc taaagaataa
    16441 gcgccgcgct cgggactgaa tttctgatgg ctgccctgga aacagggcag cctttttgtg
    16501 tctggctgca ggctcgggtt gcctggcagg atatgctgaa ggtaactgaa tcaggctgaa
    16561 gctgggaagc ctgagccggg cttgggttgg gtagtttctg cctcattatt tcccacattc
    16621 cggacgcgca aggttttggg attcgagact ggctcgtgag gttttgtttc agccttatca
    16681 gccgctgctt gaatatcgag agcgcgggtt tggggggcta ttccgcagcc cagagtgcca
    16741 ggacgtccgc gaaggcaatc ggaccttcgc gcagcagctt cagctcggcg cccgtaccgt
    16801 cgatgatggt ggaggctgtg ccgccgcgga gtctgccgcc gtccaaaatt atgtccaccc
    16861 tgccatccaa ctgcgcgcgg acttccccgg catcctgcgg gttctcgcgc tcggagatgt
    16921 tcgcgctggt gaccgccagc ggaccaaaag ctttcaacag cgtccgcgcg aagggatggt
    16981 cgggcatgcg cacagccagc ccggggtagg gcgtcaggtc tggcggcagt gaggggtttt
    17041 tgggaaggat gagcgtaagc ggtcccggcc agtatctgtg gataaggcgc tcagcttgag
    17101 gtggaagcgc tgtaacaagc ccagacagct ggctgctttc tccaatcaat acgggaatgg
    17161 ccttcagggt cgggcgattc ttggcggcgt aaatcgcctg cagcgcgttc gggttggtcg
    17221 ggtcgcagcc cagcccgtaa agggtgtcgg tcgggaaaac gaccagtcct ccgctgcgca
    17281 gagtctgcag cgctgcttcc aatgcttgcg ggtctccggt gggcagtatg cgcgtcagca
    17341 ttgcgtctcc ttctcaggcc aattgtaccc gtaccaggcg atcgcgttgg ttcaggtctt
    17401 tcagcaagtc aattctggct tcagggaaat tttcctgcgc gagcgcgagc gtttccgcgc
    17461 ccaactccgc ttcggtttcc gcaagtaaga gccctggatt ttggaggtgc ggggcagaca
    17521 ggctgaaaaa aggccgcagc aggcgcagac catcctcgcc gccgtccagc gcagtttcag
    17581 gttcgaaggc gatgctgttc actgtgccaa gtttgcgggt agggatgtag ggcaggttgg
    17641 cgcagataag gtcatacttc gggaagtttt cagggataag gtcagcctgc aggaaggtga
    17701 cgcgctcgga aacacgcagt gcccgggcgt ttgcgcgggc aatttccagc gcttcggcgg
    17761 agttatcgct tgcggtcccc tgcaagctca agacccggtt ggcaagcgtg agcgcgatgc
    17821 agcccgaacc ggtgccaaca tccagcaggc gcaccggcct gtccaggagc tgcagccacg
    17881 tcagcgcttc ctctactaac agctctgttt ccggtcgggg aataagcacc gcagaggaaa
    17941 ccttgaatgt cagcccgaaa aattcctgcg taccggtcag gtacgggagc ggttcgccct
    18001 gggcaaaacg ctgcgccaat tggctgagtt gttggcgcgc ttgcgtgttc aaacgggctt
    18061 ccgggtgcgc caacacccag gttttcggct gttgcagcac gtgcgctatc agtaccaaaa
    18121 tgcccgcgct ggcatcaggg aggtcgggag ggagttgtgt acgaattgcg tgctggagtt
    18181 ccgccaggtt cgcgtcagtc ggaagtttca tagtttgcca ggcggtcggc ttcgtcggcg
    18241 acctgcagct catcaatgaa agtatccagc tcggtgccgc tcatgacgcc cgccaggttg
    18301 aaggaggaaa ggttgatgcg gtggtcggtt acgcgcgact gcgggtagtt gtaggtgcgg
    18361 attttctcgc tgcgttcgcc cgtgccaatc tgggaacggc gggtggcgtc cagttccgat
    18421 tggcgctttt gctgttcaat ttcgtacagc cgcgcccgca atacgcccat ggcgcgcaga
    18481 cggttttgca gctgagaacg ctcatcctgg caggcgacta cgatgccggt tggcaggtgc
    18541 gtgatgcgca ccgcggtcat attcttctgg acgttctgcc cgccagctcc ggcggaacgg
    18601 tatacgtcca cctggatatc cgcatccggg atatcaattt ctacgtcctc aacctccgcc
    18661 agaacggcga ccgttaccgt ggaggtgtgg atgcgcccct gggattcggt ctcgggcacg
    18721 cgctggacgc ggtgcacgcc ggattcgaac ttcagccggg aatacgcgcc cttgcctttg
    18781 acagcgaagg ttacttcttt ataaccgcca atgccgattt cgttggcgga aaggatttcc
    18841 gtcttatagt ggtgcaggtc ggcataacgc agatacatgc gcagcaggtc cgcggcgaag
    18901 agcgcggctt catcaccgcc agtgccggcg cggatttcca tgatgacgtt gcggttgtcg
    18961 cgcgggtctt tgggcactaa cagggagcgc agttcggttt gaagagtatc gagctgttcg
    19021 ctgaggtctt gcagttccag ctcggccagt tcgcgcattt ccccttcggc ggaggcaagc
    19081 aggtgcctgg tctcttcctg ccgggattgg gcagccttgt attggcgagc caggttcacg
    19141 accggttcca attcggcgcg ctctttggag agctccgcga cgcgctggta atcgctgccg
    19201 gcttcggcaa gtttttgttc gagctcttca aagtgcccga gaatatgttc aagtttttct
    19261 tccaattgtg tttccatatg aatcgccgca ggattatacc actggaaaag aagaaaatct
    19321 acgcgcgcac gttgggtatt gtggaagagc aagatgataa tcttctgaag cagaaaagaa
    19381 gaaatgtcct gtcaggttta taaggcattt gtagtgcttc cacctgatca cgccgcgaac
    19441 tgaagaacag caggcgctgt tggttgcaga tccggctgcg ctcatcaacc ccagcaacga
    19501 atcgtatcca gacaaacgcc agcttgaaag acgccagccg ccctgcgatt cttccggcgg
    19561 cttgcaaaac cccagccgct ctgcggctta ttcgcagccc gccgcaggcc tgcacgagca
    19621 caaactgtga taaagttgtg gaaatagaag acaggtaggg atatggaact ttacttcatc
    19681 cgccacggac aatctgaaaa caacgccctc tacggggaag aagattatga catcgacgac
    19741 cgccgctctg acccaaaact taccaggctg gggaacaaac aggcgcagct ggcggctgaa
    19801 tgcttcctgc gcagcgcggc atccgaccca atcaagcgca atggctggca aaaccatgcg
    19861 gaatgcggac tgacccacct ttactgcagc ctgatggagc gcgctgtgca gaccggcagt
    19921 atcatcgcgc agacgttgaa tgtaccgctt tacggcgtgc cggatttgca tgaggttggc
    19981 ggcatttatt atgacgatat tgtcgatggc gtcattcgca tccgcattga acacggctta
    20041 acccccgctt atcttgcgca aacttatccc atgctcaccc tcctggagcc cattcctgcc
    20101 gagggctggt ggagaggcgg ccgggaagaa cgcgctgcgc gcataccgcg cgctgtgcga
    20161 atgctggatc tgctgtttgc ccggcacggc aacacacagg accgggttgg ggtgattacc
    20221 cacggcggtt tttttgcctg cctcttccgg gctatcttta gactggacct ggacgaaccc
    20281 aatcagcact tgcttcccta tcagcttgaa ctcaataact gtggaatcac gcgcttttcc
    20341 attgaggaag ctcgctacct tttggtgtat cacaaccgca cggacttcct gccggacgac
    20401 ctggttacgt gatttcggcg cgataatcca cccgcgcaaa gcatcccgaa atatctgtta
    20461 gaattgcgga tgtttcccct gacaggaaag cttcagagct cattcatgca tgccgctttc
    20521 cgggggggtg ggagaagaaa tgccagtcta tgttaatatc atcatcattg cggtgctgtt
    20581 ttactttaac ggcattttcg cgatgtacga aatcgcgatg gtttcggccc gcaaaacgcg
    20641 cttacagcag cgcagcgaag acggtaaccg cggcgcaaac gacgctttgg aactgcttaa
    20701 ggacccgaac cagcaatacc tctcgaccat tcaaattatg attacgatga tcgacacgct
    20761 ggctggcggt atcggcggcg cgatgctggc cgaaccggtc gcagaccttc tgcgcaaaat
    20821 aagctggctt gcagccggcg cggataccat cgcgttgatt ttggtcgttg tggttatcac
    20881 ttacttttca atcgtactgg gtgaattaat ccccaagcgg ctggcggtga gcaaacccga
    20941 agatgtggtc tgccggctat cgcccggaat ccgcacgatg acgcgcattt tccgcccgct
    21001 cacgcagctt ctgagcgctt ccaccaactt cggcatcaaa ctgctgcgga tcgatactca
    21061 ggccgaacca agcatcacgg aagacgaaat caaggtgttc atcgagcagg gccgcgatat
    21121 cggcttgatc gagcagactg aatcggacat gttctccggc gtgttccggt tgggcgaccg
    21181 gcgcgtggac gcgctgatga ccccgcgcac tgacctggaa tggattgata ttgaggatga
    21241 ccaaaacaca atcatccacg cattgagcaa cagcgagtat tcgctcctac ccgtggcgcg
    21301 cggcagcctg gacgacgtcc tggggatggt aaacattaag gagctggtgg gcgtggacct
    21361 gaccaagccc ggtttccgcc tggaggatta catccgcgag ccgatgtttg tgccggaaaa
    21421 tatgcaggcg ctgaaagttt tcgaacaatt ccgcgcgacc ggtacacacc aggcgctggt
    21481 catcgacgag tacggcggcg tgcagggcat ggtaacgctc tatgatgtcc tggaagcgat
    21541 tgtgggcgaa atcccgctcg atgccgaaga cctggaacag gaaattgttc agcgctcgga
    21601 cgggtctttc ttgctggacg gcatgcttcc ggttgatgaa atcaaagaat tgctggacct
    21661 ggatgagctc ccggaagaag agcgcgccgg ttatcagaca atgagcggct tcgtaatgaa
    21721 tcagctgggc agcatcccaa aaatcgggca ggccttcacc tgggaaaatt accgctttga
    21781 ggttgtggat atggacggac gccgggtcga ccgggttctg gtcacggtcc tgcctggggc
    21841 ttccgaaaca agttcgagct aaaagaccgc tacggcgtcc aacgggtata cagcgcgtgc
    21901 gaaagcaaat gcccggcgct ttcctcaacg ctcaccagct caccgcactc atatttccct
    21961 tgattatggg tcatatcctg cactgcctgc agcgccacca gggctgaggc ggaaatcgcg
    22021 taggtggtga aggcaataaa gctggccttg ggagagagta tcgcgcggca ggcttccacc
    22081 agttccggca gcttcttgaa gaagtcccag acctgcccct gggaacccct cccgaatttg
    22141 ggcgggtcca gaattagccc gtcatatttg ttcccgcgtc tggcttcgcg ctgggtaaac
    22201 tttagcgcgt cctcggcaat ccagcgcacg ggcttatccg ccagacccga gagcttgagg
    22261 ttgtagctgg cccagttgag ggcgtgcttg gaggaatcca cgtgggtcac ttccgcgccg
    22321 gctttggcgg ccgccagcgt ggccagccct gaatatccga aaagatttaa gacgcgcaag
    22381 gggcgttttt ccgactggat gcgctccata atccaatccc agtggctggc ttgctccggg
    22441 aaaaccccga tgtgccgtga ggtgcccaac tgaagttgaa aagtcaggct ctggtaatgc
    22501 atttcccagg ttttttctat cggcttgagc agctgccagg acccgccgta ctctttattc
    22561 tcctcgcgta aaaccgcgtg cgccttctgc cattccgccg cgggcagcga ctttttccac
    22621 acggcctgcg ggtccgggct gatcaacagg taggggccaa aacgttccaa ggttttcccc
    22681 tcgccgctgt ccagcagggc atattcgcgc cagttttcag aagccaggag tttgatttca
    22741 ggaagagaaa aattgggagt tggcatagtt caccattata accagcggat caggaaactc
    22801 gtcagaaatt caaccagcaa acccagtgcg accgctttct cgagcggaat atgcaagggc
    22861 aggcgggttg gctcgcggtt ggagcggaac agacccggga tgagcagaat tccgccgcag
    22921 accagcacaa aaatagccgc gcgcaggggt aaaacgcgca ggaagaaaaa caggatgatg
    22981 ccgctcagca gagctgctga ggcgcaggca atgaccagga aatgaactgc ccgcgcgctg
    23041 atgttcagga ccgtctggcg tgctggcgct tctttcagtg ccttcaggct gcttcgttca
    23101 tgcgtaattt cgcccagcag actgagaaaa atcactgtca ggaaaagaaa gagcagttca
    23161 gtggtgaagc gcggttgaaa tgccagatac ccggaaaggg aaagcgcggg tgcgagcagc
    23221 caactgtgcc cggcaaggtc cagcatagaa atgccgtgca cgcgcgtgcc gcgctcggaa
    23281 tacaacagcc cgaagcccag aagcaccaaa cccacgaaaa gcaggcgcca atccaaaagc
    23341 cagaagagcg ccagggagag caccgcagcc gcaagcgcag tcagggcagc ctctgaggct
    23401 gaaattgagc cctgcgcgat tgggttcggc gccgattttt tctggcgaaa tgtgtccgca
    23461 ggtgcgtctg ccagctggtt gaagataaac gtgaaggaag aggcaaaaaa attcgcggcg
    23521 cacaccagca cgaaacgcaa gccgaattca ccttgtgcgg cggcgatacc aagcagactg
    23581 gttgccagga cgaaaaagaa gtaatctttc ggccggacga gctgcaggaa tgcctggacg
    23641 cgtttggtca tgccatgagt ataccagtta agacccgccc aaagcggcct tgcggccgnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ggtcatgcca tgagtatacc
    23821 agttaagacc cgcccaaagc ggccttgcgg ccgagctttc gctcttttgg cgcgattggg
    23881 tatagaatat actgatatgc agaccggagg ttatcagatg gatttcgaca ggattattga
    23941 gcgcaggcat acggagagta ttaagtggaa atatttcccg gaggacgtgc tgcctttgtg
    24001 ggtggcggac atggactttt tgagcccgcc ggaagtaatc gaagcattgc atcaacgcgt
    24061 ggaccacgga atttttggct acggaattcc gccccaagac ctgtcggaaa tcgtggtgaa
    24121 ctacatctcc aggcattttc actgggcacc gctgccatca caggtaagtt acatcccggg
    24181 tgttgtgacg ggttttaacc ttgccatccg tgctttttgc gaacctggga cctcggtttt
    24241 gttccacaca cctgcctacc cgccattctt cggcgcgcct aaatctgcaa aactgccatt
    24301 tgtcaccaat tctcttctgc gcggcacgga cggcgcttac agcatcgatt ttgagcgttt
    24361 tgaggaacag attatcgcgg agcgcgtcag cgtcttcatc ctgtgcaacc cgcagaaccc
    24421 gaccgggcgg gtgttcacgc gcgaggaact gaccaggttg gcggaaattt gcctgcgcca
    24481 taaagtaatc ataatttccg atgagattca ctgcgatatt gtctacgacg gcagacagca
    24541 catcccgatt gcatcgctgg acgcagaagt ggcaaacagc actctgacgc tgatggctcc
    24601 cagtaaaact tataatatcg ccggcttata tgcgtctgtg gcggtaacca gcagccccga
    24661 attggcgcgc aagttcagtg aggtcaagaa cggactgatt agcgggccgg acctgctggc
    24721 gctgacagcc gcccgggcgg cttaccttta cggtgatgga tggctgcaag gcgtgctcgg
    24781 gtacctgcaa gccaaccggg attggctgtc ggaagcaata gccagagatt tccccgggat
    24841 gcgaatgtgc aaacctgaag gtacgttttt ggcctggctg gactgccgcg ggttgaacct
    24901 gcctctcgag ccgcagaagt tcttccagga tcgcgcccgg gtagggctca acagcggcgc
    24961 ggattacggc tctgaggggc ttgggttcgt gcgtttgaac tttggcacgc agcgggcagt
    25021 cttgcaggaa gcgctcgaga gaatgacgaa cgccctgaat tccctgtagg ggcgccgccg
    25081 gagagaataa aaaaagaccg ggaaaaccgg tctttttaac tggcagaaga agcttattta
    25141 cttgtcgagc acgacaacat tcgcggcttg tcttcctttc gggccgtctt caatcgaaaa
    25201 ctctacgcgc tgtccttctc tgagacgttt atatccctcc atctcaattg cggaaaaatg
    25261 aacgaataca tcttcgccag tttcctgacc gataaaacca taccccctgg gggcgctgaa
    25321 ccatttaatg gtaccaacta accgttctga catgcttact ccaatttctt tcaaaaaaca
    25381 aaaaaagtta acacctgcat agaatttagc acgccaacct atttctgtca agattaccat
    25441 aaatattctg acgccaaact taaaatgtcc ccttttacca aagttaaaat gtcaccatag
    25501 aggtgacaga aagtggacaa actaatgaca acaagcgaaa aagaaataac cagactgcca
    25561 atcatggtac gtttggaaaa aaggcaaata acgcaagtaa tggcgcccga acacatggag
    25621 ataagcgttg ggcaggtaaa caatcgcggc catacctcta cacagcccca ccaggcgctc
    25681 ccgggattct gtaaatgtga tacaaatatg ggcaagaagg cgtcttcctt ttatttctca
    25741 aatcaagatt taatctcaac gcaagcatcc gcggatggga tgaattatag acccagacat
    25801 gactatcaac gacccgcgct acctgaagaa atatcaatac cgcgaccctg gcaatctcaa
    25861 ctcgcgtatc acgattcata cgcgttatag cacagccagg cagtcctggg ctgattttat
    25921 cttcgagcag ctgcagcctt tggcaggcga gcgcatcctt gaattgggct gcgggcccgc
    25981 cgcgcagtgg caaaccacct tcagccgcct gcctgaaggc gcccggctga tattatccga
    26041 tttatcgctg gggatggtga aagaagcacg gcacgtgctg gcgacagcag gccgggctgc
    26101 tttcttgatt caggaagcgc aaacgctggc attctccgaa cagggttttg atgtggtgat
    26161 ggccaaccac atgctgtacc acgtgccatc acccgcccgg gctatccagg aagccgccag
    26221 agtgctccgg cgtgggggca ggttgattgc cgccaccaac ggcgcaaggc acatggcgga
    26281 gctggacgtc ttgttgcgcg aattcgagcc gcgctttgag accgaaaccg caatgagccg
    26341 ccccttcagc ctggaaaacg ggcgcgagca gctggaacaa tatttcggca gtgtggagtt
    26401 aatcccttac gtaagcgatt tgtgggtaac ggacgccgag ccattggcgg agtacgtgtt
    26461 cagcacccct accggtcaaa tctggttctg taaagaccaa atcggggata tgaccgcttt
    26521 cttccagcgc aagattgacc agaacggcgg catttatatc cataagtcta ttggcgtctt
    26581 ccgggcgagc catcccaagg cgtagttccg gggaagcgaa agcggcgctt tttcaacgaa
    26641 ttcccataac gagcaagacg gccacagcaa ccagcgcgca gtacaatgcg aagggcagca
    26701 tgctgcgcgt cttcaggaac gtcaggaacc agcggatgac aaaatacccg accaacccgg
    26761 cgacgatacc gccgagcagc accacaggca gaaatgccgc caggccgggc acggcgagaa
    26821 ggtccttgag cgccaacaca cccgcggcag tcataatcgg gattgccagc aggaacgaga
    26881 agcgcgcggc atcctgacgt ttgagcccgc ggaaaagagc gccggaaatg gtagccccgg
    26941 agcgcgagat cccgggaaag attgcgacgg cctgaaagac gccgataata aagctgtcca
    27001 gccagcttag ctgagcggtt tcacgcgtct ttttggagaa cacctcactc agcgtgagca
    27061 gcacggcggt gatgaagagc attacggcgg tcagcatcgg atcggaaaaa gccgcctcca
    27121 ccttatcctt aagcagcaga cccgctaaac cggctggtat ggtcgccagg atgacgagcc
    27181 acccgagccg gctgtctgcc tccgcgaagg gctggcgcgc gcggatgccg gcagcccagg
    27241 ctttgaggat agccaacaag tctttccaga agtaagcgat gaccgcggct aaggtgccaa
    27301 gctgaacaag cacgtcaaaa acaaaagcct gggtcgggtc gagcttccaa ttcagcagga
    27361 agggcaccaa aacgaggtgc gctgaggaag agaccggcag gaattccgtc agaccctgca
    27421 caatgccgag gattagcgct tgaatgactg tcatttttcc gtctccttcg tatttagctg
    27481 tgccagtttt tcccgggcga attgctcaaa attttcagcc agaatggcgc tgcgcatgtc
    27541 ccgggaaagc tgcacgagcg tgtgcaggtt gtggatggaa agcagcgtgg cggccagcat
    27601 ttccttcgca ttcaccaggt ggcgcaggta ggcccggctg aaatgccggc aggtgtagca
    27661 gccgcaatcg gggtcaatcg gacgctcatc gcgggcgaag ccggcgttca tcaggttcag
    27721 ccgcccgcgc atggtcatcg cgctgttgtg gcgcgccagg cgggtgggca gcacacagtc
    27781 aaaaatgtcc acgccgcgcc gcacgccttc aatcaggtct tccggcgtgc ctacgcccat
    27841 caggtagcgc ggtttatccg ccggcaggat gctgttcact tccgtgatgg tgcggtacat
    27901 ttccgtcttg gattccccga ccgagagccc gccgatggca taccctggaa aacccagcgt
    27961 gctgatttcc cgggcggatt ccgcgcgcaa atcggtgaat acaccgccct gcacaatccc
    28021 gaacagcgcc tggtcatccc gccgtttcgc ttccaggcag cgcctggccc aggcgtgggt
    28081 gcggcgcatc gcgaggcggg agtaatcgta atcggtgggc acggcgcatt cgtcgaaagc
    28141 catgatgatg tccgcgccga ggttttcctg gatgcgcata gatatttcgg ggctgaggcg
    28201 ctttaacgaa ccgtcgatat gggacttgaa tgtaacccca tcctcgtcaa ttttacgggt
    28261 ccgggtcagg gaaaagacct gataaccgcc cgagtccgtc agaatgggtc tgttccattg
    28321 catgaagcgg tgcaggccgc ccatttccgc caccagttca tcccccgggc gcagaaaaag
    28381 gtggtaggtg ttcgcgagca ccatttgcgc gcctaattct tccacctgcg cgggcgtgag
    28441 cgccttcacg gttgcctggg tgcctaccgg cgcgaaaaca ggcgtgttga gcgcgccgtg
    28501 aggggtggta aaggtgccgg cgcgggcgtt cccatccgta ccgtcgagcg tccaggtaaa
    28561 aggagtcatt tggcttcttt cgtgatgttc ttgagaagtt tgttcacccg ccgagcctgg
    28621 ggttcgctca ggtcaggaag tcgggcttgg agaacttccg cgaaagtccc ggcatacgca
    28681 ggggaaacac acgcggatat tttttcagca tgctgcgcca cgcttgcggg acggcaagtg
    28741 cgcagatggt ccaaaaggaa aggcatgatt gccagcgcgt attgcggatt tgcggcggca
    28801 ataccggaaa gcgcgccaat ccctgcatcc cgggtgatta cggacccggt ttggacgctt
    28861 tcctggaaaa gcggcacttg cgggaagagc tcgtccgccc ggattgcagc aatggattcc
    28921 aaggcgatca tgccgcccca aaccaggcga ttgttttttg cacgcagcag cttcagaaac
    28981 acttcagcgt atggagaaat cagcgcaggt tggcgtttgc ctgtctcata aagcgtctta
    29041 aggatatcgc tggagagggc tgccggggct tttggcagcg ccgtgacgag ttcccgcacg
    29101 gcttctgcat ccatggaaga cgcgattgcc tcggcaaggc gaaaattctc ttcgtcaccg
    29161 cgtttgccgc gctggcttga taatcgattc aaaacttcgt tttccatcgg aatctcgcaa
    29221 ttctctggaa ttttacctga tattccccgg ttcaaagtca atcgtgctaa ttttggcttg
    29281 tcaaggctca atagaaatgc cctaattcgt gggtgaagtc gtcaggacgg gtttttcctc
    29341 gaaactcagt cccttatttc tttggattgt ttcaccgttg cccgaaccct gacccatccc
    29401 cagcggcatc gcggctctct ttcgcctcgg tcaagccgct ggagaaagga aaaatgtttg
    29461 ctggaatcaa agattaattc cccgtcccag cgaaagtgaa gcaggatcat agaatctatt
    29521 tctttcgctg ggaaagggag taagtgggga aaggctgcca tagcattgtg tgtcagggta
    29581 ttctcattcc tttgcgaaaa aagcctggag aaatcgaatg ctgatttcat aggtcttcat
    29641 cttaatgagg atatttcgat tttgctattt cagaatctta tcctgctgct cttccgcgcg
    29701 ctttttttgg cttgttagct tctttttagg ggattatact taccccctgt ggatatcaat
    29761 agttactcaa cctggttaga aatagacctg aacgccattc gcaacaatgt tgccaggatt
    29821 aaagacctga caaaggtgga agtgatggcg gttatcaaag ccaacggtta tgggcacggc
    29881 gcgcgtccgg ccgcgcaggc tgctgtgcag ggcggggcca cctggtgcgg cgttgcccgc
    29941 atcgaagaag ccctggctct gcgacgggca ggcatctcct gcaagctgct ggtgctgggc
    30001 tacacgccgc cttccaaaat ccccgaagcc attgaccagc aaatcgtcgt ttcgctgttt
    30061 gacccggacc tgacgcgcag ctatcttgag tttgcgcgta taaacggcgg catcctcaaa
    30121 gcgcatgtga aagtggaaac cggtatgggc aggctgggta tgcttcccga agatgtaccg
    30181 gactttctga gggaactgcg cggtgggcca atcagcgtcg aaggcatctt tacgcacttt
    30241 gcccaggcgg atgaaccaca gtccgactac acctacaagc aaatccgcat tttcaatgac
    30301 ctgcttgcgc gcttggaaag ctctgagctg aaaccccagg ttgttcacgc cgcaaactcg
    30361 gcagcggtct ttaacttccc cgaagcctgg tatgacctcg tgcgcccggg cgatgcgatt
    30421 tttggcatgg cgccttcggc gcttacgccg ctgggttcgg cttttaaacc cgcgctcacg
    30481 tggaaagccc gcctgatttc ggtgcgcggc tacccggcgg gcagcggcat cagctacggc
    30541 tctcaatacg ttacgaccgg caacgaacgc atcggcgtga tgccaatcgg gtacggcgac
    30601 gggttcaggc gcattgacaa ccaggaggtc ctggtcggcg gcatacgcgt gagggtggtc
    30661 ggccgcgttt gcatggacca gtgcatgctg cagttggata aagttccgga cgcaaaagcc
    30721 ggggatgaag tggtgttgat tggcacacaa gccggggaga ccatcagcgt ggatgaggtg
    30781 gccgggcgct ggtccacgat taactacgag gttgtgtgcg gattagcgga cagacttccc
    30841 agaatatacc tcgaatgaac gcaagttttc ttagcgctga atttcactgc catacccgtt
    30901 tctctccgga tagtctgatg cgcattgagg cgctgcttca ggcttgtcgg caaaagggca
    30961 tcgataaagt ggcggtgaca gaccacaacc ggctggcagg cgcgctaaag gcgcatgaaa
    31021 tggctcccga ccaggtcatc gtcggcgagg aaatccagac aacccaggga gagattttgg
    31081 cttacttcct gagcgaggaa atcccggcag gcttggaacc agcagaagtt gtgcgccgtc
    31141 tgcgggcgca aaacgccttt atcagtgtgg cgcatccctt tgacccttac cgcggctccc
    31201 gatgggaaaa gggaaccctg gaaagcctcg tacccctgct ggacggcatt gaagtgttta
    31261 acgcccgctg cgtggaaagc gcatttaacc aacaggcgcg ggcattcgcg cgcgaatttg
    31321 gcatcccgga aatggttggt tcagacgcgc acagcgtttt tgagcttggc agagcggtgc
    31381 tgcagctgcc tgagtttcac accgcggatg aactgcggac ggctctgcgc agctctcagc
    31441 aaaagacgcg cctttcaggc agttacgtgc acctcatcag cacctgggca aaactggtaa
    31501 aacgcgttgg gaataccagg tgagcggccg atacaatgaa aagacctggg aagcccaggt
    31561 cttctttgtt aattcaacag ggtgaggatg agccaggcgg cgagtgtgag gaacacggct
    31621 gccatgccca gcttgaccgc cgcggcatgt ttcttgagga atgcggagag ctgcttggag
    31681 gtcgtaccgt aatatgccag gatgaaaacg ataatcagcg ggatgatgaa catcaggttg
    31741 tagagcagca ggtagccaat agctttgccg cgcagttccg gtacgctgct catgaagatg
    31801 atggtcggaa ggtaaatctg gcctgtgcat gccagttcca gcagcgagat gagcgcgccg
    31861 ctgacaaagg cgcccagcgc gtaactgccg atttttttcg tgccatgaac ggtcgcgtta
    31921 atccgtttgc gcagcgcttt aggcaggttg agcgccatat cttccagctc gcctttgcgg
    31981 gctgtgaaaa aatccttgat gctgagcgcc gccagcacca gacataatag cgcggttagc
    32041 gcgtatacaa ccgtgctgat gactgccaga gcgcctttga taaggtcgag caccttgtag
    32101 aaacccaacc caaccaacaa gtaggtgaga aacacgccga aggtgaaact gatgccggtg
    32161 aatatcacct ctttgccttt ctttccgctg agagtgaggt aagagatgaa gaatacgatg
    32221 gtagcaaagg cgcaagggtt caggccgtcc accaacccgg ccagcaagac cgccacccag
    32281 cctgcggact ggaagcgctc attgagcgca ccagcggctt gtttttcgtc gaacgcgtcc
    32341 caaaaacggg gtgaaccgga tgcgctcaag acctgcagga ccggttcgag gttttgtgtc
    32401 gtaatttcac cctcgccaat ccaggcctga tccccgataa aaagcgctgg taccttcagg
    32461 tcagcgctgc ggccggcccg ttccgccatc cagttcgcga gcggtgtgtt atcgtagata
    32521 ttcaattctt ccaccacaag ctgcggatag agaccgcgca gataattgat atctgcgtcg
    32581 gcgcggttgc attctttgca gccgacctga tagaaatacg cggcgtaaat cggagtgtcc
    32641 acctggcagg cttccgggtt ggaagtcgta caggcttcaa gctccccggc ggtattcgcc
    32701 ggcgttaccg agagcatcag tttggggtcg acccccggga tttccggaaa agcgatgccg
    32761 ctgcccgcga tccctgcctg aatcgcctcc gccagatgtt ctttgttctg ggcttcaccg
    32821 aggaaaattt ggccccccag gaccacggta ggaaggccgc gcgcttcggg tttcacctgg
    32881 tatgcttctt ccacggctag cagtgcacga tagtactctg gattcccgag ttcgagcaga
    32941 cgcatgtcga tttgcccggg gttctcagct tgcaagggtt ccagtatatt cgtcaggata
    33001 tcaaggcagt ggctgcacgt gttggagtag aagtaatatc cgtgcacttt tgcgctgctc
    33061 aggtcttccg tcgggttggc cgcttccgga ccaatcccct gggggttcac cgcgcgggct
    33121 gccgccgttt gcggtagcag caaagcgaag aaggaaagca gggataaaag gagggatatg
    33181 gtaaaggcgt gttttctaaa tgatttcatc gcttctcatg cagtcgagga taggtgtttg
    33241 cacttgcgcg ctcatggtca aatccaatgc agcgcgtttg gcacaacagg cgtattttaa
    33301 cacagcgccg ggcaaaaaga acctgccatg gggcaggaag tcaaacgcat atcaaattca
    33361 taaagaagga gctcagacga tttctacccg atccaggggg aaacggctgc gggcaaatga
    33421 ttccgaaagc ttttgttttc cctcttctga gatgcccgtg agcagcaggc gcaaataaaa
    33481 acttccgtct tcagccgtgg tgtgcacagt ccggatgatg ttcccgtccg cttcagaaac
    33541 caggctggca aagtacgcca gcgcgcccgg ccggttggcg gtgttcgagc ctgaaatttc
    33601 cacagtaatc catcccaacc cgacgcggga gatgccaacc agatccaacg cattttcgag
    33661 ctgcgcaagc gagattgacc ctccgccaac ggcagaatac aggtcatcca gactggcgca
    33721 ggccattaat cccagcagct gctcgacgcg gttgtgcgcc agcgcggaaa catcctccag
    33781 cgccagaatg ccgcgcttcg ccaagagggg atgaatgagg gctttgcttt tttggctggc
    33841 ttctttaagc gccacccgtg ccaggacgat gcgcaggcgg cgggctgtgg aaacgtcgca
    33901 gtggtccaac cactccggtt ttggcacctg gtgagggccg gtgctgatga cttctaccac
    33961 atcccccggg gaaagcgtgt cgtaaaggtg gcgttcctgt ccgtttacca aaactttgct
    34021 gattttgtcc aggtagaact cctgaatcgc ggcgacgccg tccagcactg tgccgccttc
    34081 ctgtaagaag cgcgtgccgc cgtaaggcgt gaggatttgg accggacggt atttgctgat
    34141 gtctttattg ttgttcacgg catagatgac gccccaagtg ttttcgcctt ccatttcttc
    34201 agtggcgata gccacttcca ctgcgccaac gcccggctgc caggtggtca cctggagggc
    34261 gcggtaaccg ttctgtgggg aggcgatgta atcgtcaaag cggtcctggt ccaggttttt
    34321 actgaagtag cgattcacac gagaaagcag cctgtaacag gctgcttcgt cgttatcccg
    34381 cacaatcatc cgaaagctga cgatgtcgtt taccgcggaa aaatcatcga gggaagtgcg
    34441 gcgggcgcgc gccatgcgct gaagcttgtc ccaggactgc caataaccgt tcaccgtaaa
    34501 gcggactgcg ccgtccaggc tttccgcgtc catcaaacgc ccgagctcgc cgaggtaata
    34561 gcggatgaaa ttcaggttca ggcgcgggtc ccggtcgatc ttggttttta cagtgtcata
    34621 ggattcaggc tcagcatagg ggaaagccat atcctcgatc cagcggcgcc aacggtagca
    34681 attgaggatg cctgccagct tgccgtagga gcggattgct tcgctggctt tctggcattg
    34741 tttggccgcg gacatgaatt gcagcgtcat taagttatgg cttttatccg ccagcttgac
    34801 cacaaaaact gcggggtcgc ggaacatggc aatttttcgc agcgtttcca gttcccgggt
    34861 actgccgtcg cgcgggttgc tcattttggt tacgccgtca atgatatggg cgatttcctc
    34921 gcctaaatca gccgggaaaa gcgattttat cttgtcaagg ctttcgccgg tgtcttcgat
    34981 ggtgtcgtgc agaagggcgg caatggtcca ggattctttg tggaccaggt tgtcgacgaa
    35041 gccggctacc cagccgcagt gcgtttcaaa gtaaggctca ccggagaggc gcagctgccc
    35101 gccgtgcact tgtttgcccc attcccaggc gcgcgcgata gcttccgtcc gggggacaaa
    35161 ctgctccaca tggaactcac ccaggacgcg cggtctaatt tcggtgatgt tttcaatctc
    35221 taccatggct tactgacctt gcgaggcgag aagaataata tccttttatt atagcttatt
    35281 ctgtccaaaa agaaaaattt tgctgtattt tcgcctgaat tgcgtttaga cggttgtcag
    35341 aaggaagagg tgcatgcagg cagaggtaat caggatgacc caaaaatagt tttttctggt
    35401 cttgtggtga aaggtgagca tgccgagcaa acccccggca gctcctccca ggatgctgag
    35461 cccgagcagt actttttcgg ggatgcgcca ctgattccta cgtgcggcgt ttttgtcgta
    35521 cccgaagagc aggaaggtaa ggatgctgag ggcgaggagg gggtagatca tgggaagggt
    35581 tccgggggtc tggaactcca attcatagga aatccatcac aaaaaaaga
//
LOCUS       DAOQAD010000008        35550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_1096_length_35550_cov_6.290914, whole genome shotgun sequence.
ACCESSION   DAOQAD010000008 DAOQAD010000000
VERSION     DAOQAD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 35550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35550
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1096_length_35550_cov_6.290914"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..1243
                     /locus_tag="PKL60_01420"
     CDS             449..1243
                     /locus_tag="PKL60_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94824.1"
                     /translation="MLASAHVALPFAKFVPGLLLCALAGGLAGWLSIRIGNWLASIIL
                     WLLFGGLLVWLVLWLPVNGSAQLLRSLSPNLTHWIEYPPIDRLAQFRVIGMIVIGIPS
                     LLCAILENTLVDQTMSAQAAGGWIAMLLAAGLLMGAAGFAADELLNRHFREPAQALSQ
                     MLDFAAENQGNSVEKTLARRMRLSVAKPLGNLVKEPRKMTLIAFDSMLGQMDFLIDFR
                     GTWARCKVIYSQPTQCDLMEELTQKYYISDGKGAFVWTNLTSPLFP"
     gene            1352..2344
                     /locus_tag="PKL60_01425"
     CDS             1352..2344
                     /locus_tag="PKL60_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW94825.1"
                     /translation="MAEKILDVQGLETQFKTPEGIVHAVNGVSIHLDEGETLGLVGES
                     GCGKSVTMLSILRLIQTPPGRIAAGKAIFKGNDLLAMSTEEIRQVRGGQISMIFQDPM
                     TSLNPVMNIGKQMAEPMLLHLGITPKEARERSIKLLESVGIPRAGERLKDYPHQYSGG
                     MRQRVMIAMALSCNPQILIADEPTTALDVTIQAQIVDLVIRLRKELGMAIIWITHDLG
                     VAASIANRIAVMYGGFIIEEATVKELFAHPSHPYTIGLLNSLPQIQRKGRQRLYSIEG
                     MPPVLLQTPKACPFAPRCHWVREKCWQQNPMLENISEDHKVACWVDTTTGSERS"
     gene            2341..3366
                     /locus_tag="PKL60_01430"
     CDS             2341..3366
                     /locus_tag="PKL60_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW94826.1"
                     /translation="MKTDNEILVKIEDLVMHFPIYRGVIRRQVGAVRAVDGVSLEIRQ
                     GETLGLVGESGCGKTTVGRTILQLYKPTSGHVYYKGTDLTRLKPEPMRLMRKDLQMIF
                     QDPYASLNPRMTVRDIIGDPMLAFGEASGKQIDERVRELLNLVRLDPDFANRYPHEFS
                     GGQRQRIGVARALAMNPSFIVCDEPISALDVSIQAQVVNLLEDLQRDLGLTYLFIAHD
                     LSMVRHISDRVAVMYLGVVVELSNRNEIYDNPLHPYTKALLSAIPVPDPSYYETRQRI
                     ILEGDVPSPANPPSGCRFRTRCPIADEKCALERPEFREVSPGHFVACIKVEMQTKTEE
                     VINQESI"
     gene            3432..5483
                     /locus_tag="PKL60_01435"
     CDS             3432..5483
                     /locus_tag="PKL60_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW94827.1"
                     /translation="MRKLYLLIALILVASLGLAACGTKTPATEAPATEAPATEAPAAT
                     EAPAATEAPVEEIVLNPYLGSNKLDGNGVPPTFFDDVHIRKAFAYAFDWDTIINEVYQ
                     GEAVQSKVLSLPGMPGYDPEAPFYSMDLEKSAEEFKLADVDKDGIPAGEDPDDIWEVG
                     FRLQMLYNTGNTTRQIMAEIMQSNLSAVNEKFSVEVLGLPWPAYLAAQRAHKIPIMTA
                     GWLEDIHDAHNWYQPYTSGTYGARQNMPADLKAQFKALLDQGVALIDPAERHAVYQQF
                     NQLYYDQVPGIPIVLVTAHAYEQKWISGRIMNPIFSGLYFKTMSKAAEAKKPDTLTYA
                     TIGDADTLDPALSYDTSSGEVIQNVYETLVFYDGVATDKFVGQLATDWTISDDGTVWT
                     FNIRQGVKFHAGGDLTPSDVAYSLQRGLLQGGYSSPQWLLAEPFFGVGVDDISLLVDD
                     TGALADDREALVAADPAKLKAACETVQSKIVADDAAGTVTLTLAQPWGPFLPTIANGW
                     GSIMDKEWVIENGGWDGTCDTWQNTYGMTSAEDPFTTIANGTGAYALDHWTQGTEIVL
                     KAFDGYWGEPAKVPNAVIAIIPEFGTRFAMLQAGDVDFMDVPVEQRPQVDPMVSEASV
                     YDAETNLYKDPVPVCTIDTDLLGLERFELCEETNDRPLRLYIGRPGLQQDVILFNFLI
                     E"
     gene            5621..6685
                     /locus_tag="PKL60_01440"
     CDS             5621..6685
                     /locus_tag="PKL60_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW94828.1"
                     /translation="MTNYIIRRLLLVPILLIGVTLIIFGMLQLLGPVERSALYVRGFP
                     KNEKQIDGLIKKYGLDKSFFEQYWRWLNGVIDPVTGKRDGGILHGDFGYSRTASQPVV
                     DIVKERFPNTVDLALWTVYPMLSVGIWLGVKAAVNQNKFVDQAARIYSIIGTSFPTFV
                     FGLLILMLLYAKAGWFYPGKMSDWVSKLVFSGQLKQYTHLLSIDALLNLLFDVFWDAM
                     RHMIMPIITLSYINWATFVRVTRSSMLETLRQDYVVTARAKGLKENDVINHHARPNAM
                     LPVVTMAGSSIVSLLGGVVITETVFNYPGIGKAAADAAANLDVVTILGLAIFTGTILI
                     LSNLIVDVIYAFIDPRVRLA"
     gene            6696..7739
                     /locus_tag="PKL60_01445"
     CDS             6696..7739
                     /locus_tag="PKL60_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW94829.1"
                     /translation="MTENNTNPTGDNRLLAETHSTRKIGWLERTVGPDNYRILKGLVK
                     TPASIIGMIFIAIFILVAIFAPVIAPPVGNDPYKMPRDGFVGEPKDPGSPWKKNVPDI
                     PFWYKSLTGNEDWVHLLGTAQGQYDIFYGIVWGTRTAFKTGFIVVLATVLIGVVLGSI
                     AAYYGGGVDNLIMRVTDIFMTLPYIMMALIFSAVLTPILGRSLLPPLIAMIAFGWMGY
                     ARIIRSDILSIKERDYVLAARASGLKNGRILFKHILPNAIFPTLVLASLGIGDVVLGF
                     AALSFLGIGTEVGYADWGQILSFARNWITSLDKYWYIVVYPGITLTLFVMGWNLVGDA
                     MRDVLDPRMRGKG"
     gene            7820..8794
                     /locus_tag="PKL60_01450"
     CDS             7820..8794
                     /locus_tag="PKL60_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNW94830.1"
                     /translation="MLRKDKSLIGSSNILGINLDGERLLVVYGSPTGTVYERLSAPIP
                     AQANFAAVLELIMVQADRLLTLTQAQHLALPERVSVAISGNLDPETGILSSAIDFPAW
                     KQEPVRSQLSLRFNLPVALEQKANAGALAEYYFGSGQNVRNLVFLSMSPTLRAGILTN
                     GRLYHNSGGTAGQLGSLRLAAEGPAGSSKPGTLSDFASAAGMLELARKRCPQHWDRDT
                     NLFQVIRDAQAGDPFAQDVFAEAGAQLGRGLAALVHLLQPDLIIVGFPGCLLEEHLLE
                     PARAALAAATELAESALPGLIPSALCARLPELEAIAPAIHQFHSQNKE"
     gene            complement(8840..9694)
                     /locus_tag="PKL60_01455"
     CDS             complement(8840..9694)
                     /locus_tag="PKL60_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNW94831.1"
                     /translation="MKQQTEVRKNDILLGSLERPALQWLAAHMPAWVNSDTLTVIGIL
                     GSLLTLVSFIIVGRTGGPLRNYWVLIACLGFVINWFGDSLDGTLARYRHMERPNYGYF
                     IDHSVDGFTALCMFLGFGFSGISRLDVTLFAAIGYLLMMITVFLKTKVTGVFEMTTIK
                     LGPTEMRLIAILFSLVNYFIGVKNVELPLFGSQTYGTLTIGIIALILYVYYLVETVRV
                     SIKLEKFDAERLAQRKEKEAKKLAKKQAKLMTKAGKKQRRLEEEMAQAQKKKKKKKHS
                     VEEMPHTA"
     gene            complement(9704..10627)
                     /locus_tag="PKL60_01460"
     CDS             complement(9704..10627)
                     /locus_tag="PKL60_01460"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNW94832.1"
                     /translation="MSVKAFFQDSGPIGLGMWLGRHCSLRAGRRLAIFAADILSTFQG
                     GKMLQAIKANQWVASGERLNRAELAARSRLVMRNIVIALFEYFYYYQHEDEARQCIIL
                     SPEMQSMLEDSAQRGKPLIILGPHMGNFDLFGMMIVKLGQKVVVLSYPKPNKTYQAQN
                     ALREQTGMRIMPINFSAFREAKKLMLNEGCALVTGLDRPIDGKEEQKYKPVFFGRPAR
                     LSTYYVRLARETGADVRVACGVTREDGRFYYDCTPPIAFEDYENLTDDIVLNAEKVLR
                     AAEKFIQASPEQWAMFYPIWPEALEQIKHLS"
     gene            complement(10641..11513)
                     /locus_tag="PKL60_01465"
     CDS             complement(10641..11513)
                     /locus_tag="PKL60_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNW94833.1"
                     /translation="MINFLREVLSYEFMRNALLAGVIVSAACGVIGTLVVLNRIVFIS
                     GGIAHAAYGGVGIAYFLGLNPMWGAVGFSLLSSLAMGYVNRKEKARADTIIGVMWALG
                     MALGIIFVSLSPGYKADLMSYLFGSILAVSAGDLRLMLVIAVVVLAFVLVFYRLLLAI
                     SFDEVFSTVRNVPVGWLSMAMIVLIGLTVVITMRVVGLILVIALLTIPPAIANLFVRE
                     MRSIMVLSTGLSLLFTISGLLISYALNLPSGAVIILVASLGYAAAAAFRQLRARGRMR
                     KEASGAKNTFEVIR"
     gene            complement(11510..12298)
                     /locus_tag="PKL60_01470"
     CDS             complement(11510..12298)
                     /locus_tag="PKL60_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW94834.1"
                     /translation="MEEKSETPVILLRNVWAGYDLDTVLEDVSFDLYQRDYVGLIGPN
                     GGGKTTLIKVILGLLPPRSGSVSVMGTSPQEGRKYIGYVPQLQVSDKAFPINVWDVVS
                     MGRIKPGLSSLRLSSEDRQRVEEALRQTGMLDLSKRSINEISGGQRQRVYIARALTAQ
                     PRILLLDEPTASVDPQASVQLYELLARLNEEISILLISHDMTAISAYVKTIGCINRRL
                     VYHRDKSISADMLQAGYECPVDLIAHGMPHRVFPAHTEDGEPLE"
     gene            complement(12309..13181)
                     /locus_tag="PKL60_01475"
     CDS             complement(12309..13181)
                     /locus_tag="PKL60_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNW94835.1"
                     /translation="MKTLGIIILCAALLLSACSPAAPEQAQQMAVTVSVLPQAYFVQK
                     IAGDLVSVQAVVGTGDDPHTYEPTAEQMRALTKSRLYFSIGVEFEQAWLPRFSAANPG
                     LEIVDSGAGVERLAISPTLGESAEEAENDPHIWFSPTRVKIISRNIADALAAADPTHA
                     QSYRANLEAWLKEIDAVDADVRAALAGISRSSFLVIHPAWGYFASEYGLTMLSVESHG
                     QEPGPEELGALLNLARENGITTVFVQKGISPKLAQSIAGQLGGARVVELDPMAADWSA
                     NMRLLAAELAEALR"
     gene            complement(13178..13615)
                     /locus_tag="PKL60_01480"
     CDS             complement(13178..13615)
                     /locus_tag="PKL60_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNW94836.1"
                     /translation="MDWQTFLSKAGYRLSEPRKRIMTLMEVAERPLNPLTIHRLLCEQ
                     GVHLGLVSVYRTLELFFSLGLISQVFQQDGLVGYLPASFGHHHFIVCQNCHKAVEFMG
                     SEDMRELVQRVQNETRYVVREHLLQFYGLCPECQALLEAGETK"
     gene            13783..14298
                     /locus_tag="PKL60_01485"
     CDS             13783..14298
                     /locus_tag="PKL60_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNW94837.1"
                     /translation="MTENILNAVQDGTVVQLAYNLVVDGEEIESEVLEYLHGHKNIIP
                     GLENALTGMEVGDSKEVLVLAQDAYGEFDPDAVVLVTRESFPPDFEILLGEPMHLRDA
                     DGHVFTGVATALTDENVELDLNHPMAGKDLQFTVTILGIRPATEQEIQNGGLSHSCES
                     CGEGCSDGCCG"
     gene            complement(14369..15595)
                     /gene="pepT"
                     /locus_tag="PKL60_01490"
     CDS             complement(14369..15595)
                     /gene="pepT"
                     /locus_tag="PKL60_01490"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002448057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNW94838.1"
                     /translation="MPAVLERFLRYVQIDTESDRDSETYPSSARQLDLLRLLAEELRS
                     IGLSDVRMDQFGYVTAALEATASAPAPVIGFLAHVDTSPDFSGKDVRPQIVENYDGGD
                     IILNAAEQIVLSPQRFPDLLKYRGESLVTTDGTTLLGADDKAGIAEIMTAVAYLAAHP
                     EIPHGKIRVAFTPDEEVGAGVEHFDVGAFGADFAYTVDGGEIGELEVETFNAASARLQ
                     VRGRNVHPGTAKGVMLNALQIAMEYNALLPVFERPEYTEKTEGFFHLVLLNGEVESAR
                     MEYIIRDHDDRRFQDRKALMQSAANYLNQKYGEAVVELTLKDQYYNMKKMIDPHPQIL
                     ALARAAYEALGIEPRVVPVRGGTDGSRLSYMGLPTPNLFAGGHNMHGRYEYVPVSSLE
                     KAAQVIIKICELAAAG"
     gene            15735..16568
                     /locus_tag="PKL60_01495"
     CDS             15735..16568
                     /locus_tag="PKL60_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HNW94839.1"
                     /translation="MKKTKNTPKPKSYRLFIALAALALFLLVMHVRLPGSNSIPRKLG
                     ANAEIKVLAQTPHDPQSFTQGLLYADGLFYESAGLYGQSTLRKLEVESGAVLLEERPD
                     EQYFAEGLTLLNGKLYQLTWKENKGFIYDPQDFSLLGTFTYSTEGWGLTTDGSSLILS
                     DGSAALYFIDPQSMLTTRTLQVTLDGAPLERLNELEYIRGEIYANIWYKDLIVRINPE
                     SGEVIGVIDCSILREGQSAPLPKDVLNGIAYDAKEDRLFITGKNWPWIYEVSLETVKT
                     P"
     gene            complement(16572..17594)
                     /locus_tag="PKL60_01500"
     CDS             complement(16572..17594)
                     /locus_tag="PKL60_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNW94840.1"
                     /translation="MTELPAAPLGAVIGGRLQRDFILPFEGKPRLNALGGSLPYAAAA
                     FAFWGGRAGLYARVPEGFPLEQLDTLRQKGCDLNGIVRVRERIDDRRFVAYTDPLSPH
                     TDNPITYFAERGITFPRELIGYDSASSPFCSKLEYENFTARVNDLPRSYLDAPAAHLC
                     PIDFLSHKILPSLLKAGMVETLTMRACGCYMDSSFWEEMRGLVADLTAFMLTEAEALR
                     LFRGRSVDLWEIAEALADYGPEFVLVHLPDGSVRLFDRLGQKRRVIPAYPAKTVDPTG
                     MLDAFDGAFLLNYRKKYDPVEAALCGNVTAAVCMEGFGPYYVLDTLPGLMNARLDVLR
                     GRVSSA"
     gene            complement(17600..18433)
                     /locus_tag="PKL60_01505"
     CDS             complement(17600..18433)
                     /locus_tag="PKL60_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNW94841.1"
                     /translation="MDAEMYRVPLKGKIKLKDYDPNDSKLFEGNKKDGKEALQKLNAE
                     LETLQEELYAQGKHRLLIILQAMDTGGKDGVIRAVFEGVNPQGVRVASFKTPTPVELA
                     HDYLWRVHQQSPAKGEMVIFNRSQYEDVLVVRVHDLVPEDVWSKRYQHIREFERMLAE
                     EGTTILKFYLHIDLQEQAERFLARVEDPTKQWKFNPGDLDERARWEDYMKAYEDMLNM
                     TNTAWAPWYIIPSNKKWYRNWLISKIVIKTLKNLDMRYPTPVENIEDYHKRLLEMVAA
                     K"
     gene            complement(18602..19006)
                     /locus_tag="PKL60_01510"
     CDS             complement(18602..19006)
                     /locus_tag="PKL60_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNW94842.1"
                     /translation="MDRFLDMNADAWGSQPLGFPLDVIEKDDEFLVRADAAGFDPELL
                     EITYDNNTLSIKGEIREENKEEKEGKYHVQERRVGTFYRSVSLPGQIEQGKISAETEN
                     GILTVHLPKKPEAQPKRIEIKPKSIRVVDQGK"
     gene            19836..21470
                     /locus_tag="PKL60_01515"
     CDS             19836..21470
                     /locus_tag="PKL60_01515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNW94843.1"
                     /translation="MKEKKLRIIPLGGLGEVGKNMTVYEYGGEILVVDAGMMFPDNDM
                     IGIDYIIPDFEYIKQRADHVRGIVITHGHEDHTGAIGHLLESVDAPVYATPLTLGMIG
                     AKLNRRGFEHKNARLQTLKAGETVQIGPFKVHAFHVCHSVPDGIGLGIESPAGLVVHT
                     GDYKFDHTPVDNWPTDYAKLAEFAQKGVLALLSDSTNAERPGWTPSEKVIDSAFDRVF
                     REAKGRIIVATFASLISRMQQVINAALRYHRKVAFVGLSMTENVKIARQLGYIDFEED
                     VVVPVETALQMPDSQVVLLVTGSQGEPTSILGRLANGSNKRFDIKEGDTVVLSSHPIP
                     GNEELVYRAINRLLEQGAQVIYEAIAPVHVSGHASQEEIKLMLHLTRPRYLIPIHGEL
                     RMLNQHARLGMEVGMDPRQIAIVENGRVIEFEHGEMKLGERIPGGYVFVDGTGVGDVD
                     RSVMREREMLSQDGIVLLNLIKDKSNLKEVEIISRGFMSPTESQDLFEELRQRIEKLA
                     RSANGNLERDVLNLAKSHIFDETKRRPVVFVNISKA"
     gene            21671..22486
                     /locus_tag="PKL60_01520"
     CDS             21671..22486
                     /locus_tag="PKL60_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNW94844.1"
                     /translation="MFRTTAYRHPSEFPILLLTILISIAAVVVTAFLTQCLSLLVIVF
                     ILGASIFSSRAMQAQLMRKAFLVSPERSPELASVVQLCKKRLQPGSLEVYVVRENVVN
                     AYTFGLAETKTIVLYEPMLRIMSGGELAFVLGHEMGHVSLGHTWLNTIVGGLAGSPSP
                     LGLGYIMYLVFLRWSRACELSCDRAGLLACGSLNIAVSALVRLAAPKIQTPEEFEQAL
                     TMIDSQDDVFVNRLVELFQSHPLIIKRINALKEYAASPEYASLQANVDKNLQA"
     gene            complement(22719..23030)
                     /locus_tag="PKL60_01525"
     CDS             complement(22719..23030)
                     /locus_tag="PKL60_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNW94845.1"
                     /translation="MPIYTYHCENCGVRFDQQQKFTDEPLTICPECNEPALRKIYQPV
                     GIVFKGKGFYATDHRSPSGQNHTHTRPEGATESKPDEVKPAALSPSTEALKNNYTKAA
                     E"
     gene            23176..24135
                     /locus_tag="PKL60_01530"
     CDS             23176..24135
                     /locus_tag="PKL60_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNW94846.1"
                     /translation="MKRLFLLLGTAVLLGGCVFSISPAPTNLPRPTNTALPQPTETPA
                     PPTTAPTLTPSPTPTGTPVYYQVDADDDMFGVALRYGISLEALMTANPTVIPYAMGVG
                     TLLLIPITQTPPAPTSTPAQAGQQTPEPGASLQTDCYRDLAGGVLCFALALNDTEKDL
                     ENVSAKVTLSGVDGAYTRTQSAVLPLNLLKAGQSLPLLTYFQAPTPEQFSASAAIDFS
                     LPVPEDDTRYIPAEATDLEVICARGNLSCAVNGRVALQPDSAQAGALWVLVVAYSQDG
                     RVVGIRRWDDQSSPISLDFSVLLYSAGPEIARVEVTAEARPAQ"
     gene            24144..25007
                     /gene="folP"
                     /locus_tag="PKL60_01535"
     CDS             24144..25007
                     /gene="folP"
                     /locus_tag="PKL60_01535"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNW94847.1"
                     /translation="MLSDLLPNLGKQTYIMGILNLTADSFSGDGLLQQENPLRAAVRQ
                     ARGFVADGAHILDIGAESTRPGARPVPAEQELERLLPVLRALREELPDAVLSVDTYKA
                     VVAEEALRAGAHIINDVWGLRADPRMGAVAAAGAASVVLMHNRSHPQNAQIDVSLGGR
                     YVDVSYGDVCTEVAEGLRQSVQIAREAGIDGGQILLDPGIGFGKTTEQNLRLLKQLDR
                     LKALGFPLLLGISRKSFIGYTLNLPPEERLEGSLAANAYGVLHGADILRVHDVRQTAR
                     LARMLDAIRFA"
     gene            complement(25016..26254)
                     /locus_tag="PKL60_01540"
     CDS             complement(25016..26254)
                     /locus_tag="PKL60_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HNW94848.1"
                     /translation="MVKILHFADAHIDIATHGPYDPDEALFVRVKDFLKSLDAIVAAA
                     IDEKVDLVIFAGDAYKDRSPAPTYQRAWGSRIMRLSRAGIPTILITGNHDTSPSSKKA
                     HAIQEFSTLDVPHVHVIDRPQVLGPKDLEGLPVQVLGIPWITPSLVKNSAPEAADPKA
                     ELDVIIENMVNVLIQQALAQRDPELPFILAAHASIRGALLGEERNIKLGKDVQLSQAL
                     VHDERIDYVALGHIHRFQDLNLGSHPPVIYAGSIERVDFGEVGDDKGFVIAEVSRGET
                     HYTWHKLATRPFRDFNVSIRAGENTMEKILAVFPQPEELEGAIVRLNVSYPDNIEATI
                     DDAQIRERARLALELKINRMPKFNLRARLGENGETQNMTPEELLKLYWKQSHTDEAEI
                     EALQEMAERIIREDAEDDQS"
     gene            complement(26284..27639)
                     /locus_tag="PKL60_01545"
     CDS             complement(26284..27639)
                     /locus_tag="PKL60_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNW94849.1"
                     /translation="MANKNKKKIQRKLSAKAGARWLWITLAAAAALTLLLIALYQIPA
                     IQNHAYYYVAKAHARIQYFFKPPGEKAFNPGGKGTLQPEVAATLTAMAPTPTPTLVPS
                     STSTPHPTEQATFPPTATQPPTPTSTPIPRSKALTGIKYERQRFNNCGPTNLAMLLSY
                     WGWEGDQHTVEAVIKPLKEDRNVMPYEMLEYVNAQTSLTGIVRYGGDVDMVKKLVAAG
                     FPVLVERGYMNAKEGWMGHYGIITTYDDATKKVNIPDSFLGDIALPYDLLSMYWNQFD
                     GIYLVVYPYEREEEVINILGPQWDADYNLRYALEKTSERIDTLEGRELFFAWYSRGNI
                     LIELKDYAGAADAYDEAFAVYDEIPTSDRPWRVFWYQTGPFFAYFYTARYNDVLNLAN
                     YVLDITPEDAIPETWVWRGRANKMLGNWPAAESDFRKALEWHPEWWVAESELINMGLT
                     P"
     gene            complement(27881..29779)
                     /gene="dnaK"
                     /locus_tag="PKL60_01550"
     CDS             complement(27881..29779)
                     /gene="dnaK"
                     /locus_tag="PKL60_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNW94850.1"
                     /translation="MSKIIGIDLGTTNSVVSVMEGGSPTVISTSEGSRLLPSVVAFNK
                     NGERLVGIPAKRQAVVNPENTVYSIKRFMGRHYDQVASERKMVSYKVVSGPGGDVRIE
                     IPVNGQTYTPQEISAMILGKLKSDAEAYLGEKVTQAVITVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTASALAYGLDKKDNEIILVFDLGGGTFDVSLLEVGGGVVEVKATNG
                     DTHLGGDDWDEVIVNWAADEFKREQGIDLRSDRQALQRLREAAEKAKIELSSVMETEI
                     NLPYITADSSGPKHLLLKLSRAKFEQMTDHLLQRCKLPFNAVIKDANLSVSKINEVVL
                     VGGATRMPMVQELVRQLTNGKEPNKGVNPDEVVSIGAAIQGGVLGGEVKDVLLLDVTP
                     LSLGVETLGGVMTKLIERNTTIPVKKTEVFSTAEDSQTSVDIHVLQGERPLAGDNMTL
                     GRFRLDGIPPAPRGIPQVEVTFDIDANGILSVTAKDKATSKEQKVTITASTNLNKNDV
                     ERMVREASQHKEEDDKRKALIEARNIGDSTAYQAEKLLRDLGDKADAGKRGELEGKIK
                     ELRDSLTSENSSAILSATQAVQNLMQTIGAAAYEQPQSGPTAAESQPNTGPAQGGEDV
                     VDGEFHEA"
     assembly_gap    30090..30189
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            30394..30606
                     /locus_tag="PKL60_01555"
     CDS             30394..30606
                     /locus_tag="PKL60_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNW94851.1"
                     /translation="MSKMKSWMTGAILGGLLGSALVLLYTPAKGSELKSKLQGAVQKM
                     RDEVRLAGEEKRAELEAQLDALRSGE"
     gene            complement(30628..32460)
                     /locus_tag="PKL60_01560"
     CDS             complement(30628..32460)
                     /locus_tag="PKL60_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94852.1"
                     /translation="MGAKTNTRNVVLSRIFLVCVFVLLVLNPKPAASVPLPSEGPQRS
                     VVLDVDYTTYEWWLLSWKTSQVVCQIYVEHESWPDDSEVLYYCGSTILSQWQRTSACV
                     YDDLITNPQQCPGLYLHLANVTPSTRKVEVKLPPAEVWISIVGCGNAPGDNQCSSVPF
                     LRMEAVEPLPNEQIIQILGTLNGQPFTCPGGICDLPLAPTGMNGVEVVFWAESSYGDA
                     SEKFTAQVRVIPWGEFANPEAASADQPVYYVDVLSSQYQQDNLSSCSNIWQAFLPVDG
                     APTWLNTPLLSENLVSFDEYYLLAGSLIKQGLVDASACERGGLESNGAATVCGMELAR
                     PYVNEWQNRFNAEILQVANGTGVPAQLMKNIFSRESQFWPGIYARVQEAGLGQMTELG
                     ADAVLLWNPEFFTQFCPLVLAEETCQRGFGNLDEAQQAMLRGALVQKVNASCLDCPVG
                     IDLTQANFSISVFARGLLANCEQVNQIIYNATGKSAGQLSTYEDLWKFTLLNYNAGSG
                     CLSRAIRLTVTNKQPLIWANVLQYLEPVCQAGIAYVDDISNMPVVEVPGIGDLLATPN
                     PEVVLVTPTPVPTLMPTITPTSIYPSPTPPGYPVHTPTPLPDYP"
     gene            complement(32488..33741)
                     /locus_tag="PKL60_01565"
     CDS             complement(32488..33741)
                     /locus_tag="PKL60_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNW94853.1"
                     /translation="MRAPRTLPSQYLSYARCFLALLALTAALTGCLRAVGTPTPLAFP
                     TPTDAAAAASDTPTPEVWNTFAPASIAPVTPIPPAAERMALPQDVDVWLFLGSDQEAP
                     YRGRTDAFHLAFVNTRLSKASVISIPGSLFVFIPGYTMQRLNTAYALGGMNLVRETLA
                     YNFGIKPDRYVVAHPEEFGWLVDDLGGVDVSVLLPIRDACGGLPAGLHSMNGQKALCY
                     VSYLSGEDEIDRTRRQQQMLQLLFTKLVQNGRLVKLPAMYVSYKDQLDTDFSLPELLL
                     DIPLFLRLGDPQRLSYSLLGWNELSSWELPDNTQTSVLLPDAEAVNAVFARALEDVLQ
                     SSPLGEIVLTYEAQLTEASAFSPTPLGPTATGGFPVITGTPTPTRTPLPGVTPTPGVT
                     RSPIPTAIFPTPYPVNTPTDSGEYP"
     gene            complement(33887..34768)
                     /locus_tag="PKL60_01570"
     CDS             complement(33887..34768)
                     /locus_tag="PKL60_01570"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNW94854.1"
                     /translation="METEFYPSKRPGLWVSAVLFAADLALLFLLVFNPRGIGIQLLLP
                     WRILAGVLLVGLLVGLASRVYRLLTTRYILGRGGLALRWGLRREVIPMPAVQWIRPVS
                     DFQTRLPLPPGALPGMIFGSRTVPGLGAVEYAAADPRALLLVASSGRYYAISPSEPEA
                     FLSLYERLSELGSLVNYEPRSESLGSLRKKIWQDKAARLLLAGGLAAGLALLALALAL
                     AGSRAEITWTTLEQVPSNQVFLLAFFSLFIWLVNCGIGLFFSLRGGMEKAVIYLLWAW
                     SILISLLLAAAMLILAL"
     gene            complement(34854..35393)
                     /locus_tag="PKL60_01575"
     CDS             complement(34854..35393)
                     /locus_tag="PKL60_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNW94855.1"
                     /translation="MQNEIIISGFGGQGTLYAGQLLAYAAMDEGKHVTWIPSYGPEMR
                     GGTANCTVVISDQEIGSPTVKYPKAVLAMNLPSLDKYEPLVVAGGCLIVNQSMVNRAV
                     TRTDLRAVALPANAIAAEIGNERATNMVMLGALMANMDILPEGALERALKAHTAQRLQ
                     KFVDMNTRALARGAEYKAR"
BASE COUNT         7490 a        10502 c         9812 g         7646 t
ORIGIN      
        1 ccgacaagct ttggccagcg ggtttgtgga gccatcgttg ggttccgctc ctgcgccgct
       61 gcacccggct taaacggctt gtcatcttaa gcgccagtga aggatcttct tttataacta
      121 cagatcggca aaaggaagtg cggtgcgctg ccggcggttt cgctattacg gtctgccaga
      181 gcgcttccgc gggtgatatt ttcgtctgac cgtggcagat ttaccattct gcttaaaatc
      241 agcaaaattc cctgtgttta cattttacct tcgcgcaatt cagcaacgct gttgacggaa
      301 aatggatcat cgcccggatt atgaaatatc tactataata ttcaatatga cagcgtcgtt
      361 tgcaagaaaa aaacaacgca taggtttttt ctacggtctg gttgcggggg cagtcttctc
      421 aatcactgcc tggggggtag acgcgataat gctggcaagc gctcacgtgg ctttgccatt
      481 cgcaaaattc gttcccggcc tgcttctctg cgccttagct ggcggattgg caggctggct
      541 ttcgattcgc atcgggaact ggctggcatc catcattctc tggctgctct ttggcggtct
      601 gttggtctgg ctggtgctgt ggctgcccgt caacggcagc gcgcagctgc tgcgctccct
      661 gagccccaac ctgacccact ggattgaata cccacccatt gatcggttgg cccaatttcg
      721 cgtgataggc atgattgtca tcggcatccc cagcctgctt tgcgctatcc tggaaaacac
      781 cctggtggat caaaccatgt ccgctcaggc cgccggaggt tggattgcca tgttgttggc
      841 agcggggctg ctgatgggcg cagcgggttt tgccgcggac gagcttttga accgccattt
      901 ccgcgagccc gcgcaagcgc ttagccagat gctggacttt gccgctgaaa atcagggaaa
      961 tagcgtggaa aaaaccctcg ccaggcgcat gcgcctgagc gttgccaaac cgctcggaaa
     1021 cctggtgaaa gaaccgcgca aaatgacctt gattgccttt gattccatgc tcggtcagat
     1081 ggactttctg atagatttcc gcggaacctg ggcgcgctgt aaagtcatct attcccaacc
     1141 gactcaatgt gaccttatgg aggagttgac acagaaatac tacatctcag atggaaaagg
     1201 cgcctttgtc tggacaaatt tgacgtctcc cctgttccca taatgcggtt ataatgaatt
     1261 ggtgcaaaaa cttaccaaaa aacctttggc aatgagtaat gttacttttt ttccacaagc
     1321 tttaacaaac tgatgtaaga agggctcatc gatggctgaa aaaattcttg atgtgcaagg
     1381 actggagacc caattcaaga cgcctgaagg cattgttcat gctgttaacg gggtttctat
     1441 ccaccttgac gaaggggaaa ctctgggatt ggtcggggag agcggctgtg gaaaaagcgt
     1501 taccatgctt tctatcctca ggctcatcca aacccctccc ggtcggattg ccgctggtaa
     1561 agccattttc aaaggtaacg acctgctggc aatgagcaca gaggaaatcc gtcaggtgcg
     1621 cggcgggcaa atcagcatga tttttcagga cccgatgacc tcgctgaacc cggtaatgaa
     1681 catcggcaag cagatggccg agcctatgct gctgcatctg ggcatcaccc ccaaagaagc
     1741 ccgcgaacgg tctatcaagc tgctcgagtc agtcggcatc ccgcgcgccg gggaacggtt
     1801 gaaggattac ccgcaccagt actccggcgg tatgcgccag cgcgtgatga ttgccatggc
     1861 gctgtcctgc aacccgcaaa tcctcatcgc ggatgaacca accaccgccc tggatgtgac
     1921 catccaggcc cagattgtgg acctggtcat ccgcctgcgc aaggaattgg gcatggcgat
     1981 tatctggatt acgcacgatt tgggcgtagc agccagcata gccaaccgca tcgcggtcat
     2041 gtacggcggt ttcatcatcg aagaagctac ggtcaaagaa ctcttcgccc acccttccca
     2101 cccatacacg attggtctgc tgaacagcct gccgcaaatt caacgcaaag gccgtcagcg
     2161 cttgtattcc atcgagggca tgccccctgt tcttctgcaa accccgaaag cctgtccatt
     2221 cgcgccccgc tgccattggg tgcgggaaaa gtgctggcag caaaacccca tgctggaaaa
     2281 catcagcgaa gaccacaaag tggcctgctg ggtggatacg acaacgggaa gtgagcgttc
     2341 atgaaaactg acaatgagat cctggtcaag attgaagatt tggtgatgca tttcccgatt
     2401 tatcgggggg taattcgccg gcaggtcggc gctgtgcgcg ccgtggacgg cgtctcgctc
     2461 gaaatccggc aaggcgaaac cctgggtctg gtcggagaat ccggctgcgg caaaactacc
     2521 gtcgggcgca ccattctgca gctctacaag cccacatccg ggcatgttta ctacaaaggc
     2581 acagacctga ccaggttaaa gccagagccc atgcgcttga tgcgcaaaga cctgcaaatg
     2641 attttccagg acccgtatgc cagcctgaac ccgcgcatga ccgttcgcga catcatcggc
     2701 gaccctatgc tcgccttcgg cgaagccagc ggcaagcaaa ttgatgagcg cgtgcgcgaa
     2761 ctgctcaacc ttgtccgcct ggacccggat ttcgcgaacc gctacccgca tgaattctcc
     2821 ggcggtcagc gtcagcgcat cggcgttgcc cgcgcgctgg ccatgaaccc ctccttcatc
     2881 gtctgcgatg aaccgatatc cgctctggat gtttccatcc aggcgcaggt ggtcaacctg
     2941 ctggaggacc tgcagcgcga cctcggcctc acctacctct ttatcgcgca cgacctgtcc
     3001 atggtgcgcc atatcagcga ccgcgtcgcg gtgatgtacc tcggggtggt tgtggaactt
     3061 tccaaccgca acgaaattta cgacaatccc ctgcatcctt acaccaaggc tctgctttct
     3121 gctattccgg tcccggatcc cagctattat gagacccgcc agcggattat cctcgaggga
     3181 gacgttccct cacctgccaa tccgccctcc ggctgccgct tccgaacccg ctgcccgatt
     3241 gccgatgaaa aatgcgcgct cgagcggcca gaattccgtg aagtttcacc agggcacttt
     3301 gtggcttgta tcaaagtgga aatgcaaacg aaaactgagg aggtgatcaa tcaggaaagc
     3361 atctaaattt tttgttgttg tatcagtctg ccaaaaacaa ttcagaccat tctctcaagg
     3421 aggagaataa aatgcgtaaa ctgtatttgc ttattgcact gattttggtt gcgagcctgg
     3481 gtctggctgc atgcggtaca aaaacccctg ccaccgaagc ccctgctacc gaagctcccg
     3541 ccaccgaagc acccgccgcg actgaagcac ccgccgcgac cgaagccccg gtggaagaaa
     3601 ttgtcctgaa cccgtatctg ggctcgaaca aactggacgg caacggcgtg ccccccacct
     3661 tctttgacga tgttcatatc cgcaaagcct tcgcgtacgc ttttgactgg gacaccatca
     3721 tcaacgaagt ctaccagggc gaagctgttc agtccaaagt tctatccctg cctggcatgc
     3781 ccggctacga ccccgaagcc cccttctatt ccatggacct ggaaaaatcc gccgaggaat
     3841 tcaagctggc tgacgtcgat aaagacggca tccccgctgg cgaagacccc gatgatattt
     3901 gggaagtcgg tttccgcctc cagatgctgt acaacaccgg caacactacc cgccagatta
     3961 tggccgaaat tatgcagtcc aacctctctg cagtaaatga aaaattctca gtcgaagttc
     4021 ttggtctgcc ctggcctgcc tacctggccg cacagcgcgc ccacaaaatc ccgatcatga
     4081 ccgccggctg gctggaagat attcatgacg cccataactg gtaccagccc tacacctccg
     4141 gtacctacgg cgcccgccaa aatatgcccg ctgacctcaa agctcagttc aaagctctgc
     4201 ttgaccaggg cgtcgcgctt atcgacccgg cggaacgcca tgctgtttat cagcagttca
     4261 accagctgta ttacgatcag gttcccggca ttccaatagt ccttgttact gcccatgctt
     4321 acgagcagaa gtggatttcc ggccgcatca tgaacccgat cttctccgga ctgtacttca
     4381 agaccatgtc caaggccgca gaagccaaga aacccgatac cttgacctac gcgaccattg
     4441 gcgatgccga tacgctcgac cccgccctca gctacgatac ctccagcggt gaagtcatcc
     4501 agaacgtcta cgaaaccctc gtattctatg acggcgtagc taccgacaaa ttcgttggtc
     4561 aactggccac cgattggact atctccgatg acggtactgt gtggaccttc aatatccgcc
     4621 agggcgtcaa attccacgct ggcggcgatc tgaccccctc cgatgtggcc tattcactcc
     4681 agcgcggcct cctgcagggc ggctattcct ccccgcagtg gctgttggcc gaacccttct
     4741 tcggtgtggg cgtggatgat atctccctgc tcgtcgatga taccggcgcg ctggctgacg
     4801 accgcgaagc tctggttgct gccgatcctg ccaagttgaa agccgcttgc gagaccgttc
     4861 agtccaagat tgttgccgac gacgctgccg gcaccgtgac cctcaccctt gcgcagcctt
     4921 ggggtccttt cctgcccacc atcgcgaatg gctggggttc aatcatggac aaagaatggg
     4981 tcatcgagaa cggcggttgg gacggcacct gcgatacctg gcagaatacc tacggtatga
     5041 cctccgctga agatcccttc accacgatag ccaacggcac cggcgcttac gctctggacc
     5101 actggaccca gggcaccgag atcgtcctca aggccttcga cggttattgg ggcgagcctg
     5161 ccaaggttcc gaacgcggtg atcgcgatta tccccgaatt cggcacccgc ttcgcgatgc
     5221 tccaggctgg cgacgtcgac ttcatggacg tcccggtgga acagcgcccg caggttgacc
     5281 cgatggtgag cgaagcctcg gtttacgacg ctgagaccaa cttatataaa gaccctgttc
     5341 cggtgtgcac gattgacacc gacctactcg gcctcgaacg cttcgaactc tgcgaagaaa
     5401 ccaatgaccg cccgctgcgc ctgtacatcg gccgccccgg tctgcagcag gatgtaatcc
     5461 tgttcaactt cctgattgag taggacctcg gacaggcaaa cagctgatac aaattgcggg
     5521 agcctgaaag ggctcccgca atccccgcag ttttttccct ttgccgtgca cagaaggctt
     5581 ctgtccgcgg caaaaggcaa aacaaagtga gaggtatcca atgaccaact acatcatccg
     5641 gcgtttgctc ctggttccaa tcctgctcat cggtgtgacc ctgatcatct ttggcatgct
     5701 gcagctgctg ggtccggtgg aacgatcggc gctctacgtg cggggcttcc ctaagaacga
     5761 aaaacagatt gatggtctca tcaaaaaata cggtctggac aagtctttct tcgagcagta
     5821 ctggcgctgg ttgaacggcg tgattgaccc tgtcaccgga aaacgcgacg gcggcatctt
     5881 gcacggggat tttggctatt cccgcacagc ctcccagcct gtggtggata tcgtcaagga
     5941 acgcttcccg aataccgtgg acctggcgct ttggactgtc tacccgatgc tctcggttgg
     6001 catttggctg ggggtcaagg ctgccgtgaa tcagaataaa tttgtggacc aggcggctcg
     6061 catctattct attattggca cctctttccc taccttcgtg tttggtctgc tcatcctgat
     6121 gctgctttac gccaaagccg gttggttcta tccggggaag atgtcggatt gggtcagtaa
     6181 actggtcttc tccggccagc tgaagcagta tacgcatttg ctcagcatcg acgccctgct
     6241 caacctgctc ttcgacgtgt tttgggatgc catgcgccac atgattatgc ccatcattac
     6301 cctcagctac atcaactggg ccactttcgt ccgcgtcacg cgttcctcca tgctggaaac
     6361 cctgcgtcag gattatgtcg tgaccgcccg cgccaagggc ttgaaagaaa acgacgtgat
     6421 taatcaccac gcccgcccga acgcgatgct gccggtagtg accatggcag gctccagcat
     6481 cgtcagcctg ctcggcggcg tagtgatcac cgagacggtc ttcaactatc ccggtattgg
     6541 caaagccgcc gccgacgccg ccgccaacct ggacgtggtc accattcttg gtctggcaat
     6601 tttcaccggc accattctca tcctctccaa cctgattgtg gatgtaattt acgcgttcat
     6661 cgacccgcgc gtacgattgg cataaggagc agcgcatgac cgaaaacaat actaatccga
     6721 caggcgacaa cagactcctg gctgaaaccc actccacccg caagattggc tggctggagc
     6781 gcaccgttgg gccggataac taccgcatcc tcaagggttt ggtaaaaacg cctgcgtcca
     6841 tcatcggcat gatttttatc gcgattttca tcctggttgc cattttcgcg ccggtcatcg
     6901 cgccgcccgt gggcaacgac ccttacaaaa tgccgcgcga cggctttgtg ggcgaaccga
     6961 aagaccccgg ctccccctgg aagaaaaacg ttccggatat tcctttctgg tacaaatccc
     7021 tcaccgggaa cgaagactgg gtgcacctgc tgggcaccgc ccaaggccag tatgatattt
     7081 tttacggcat tgtgtggggt acgcgcacag ccttcaagac cggctttatc gtagtgctgg
     7141 ctacggtgct gattggcgtg gttctcggtt ccatcgcggc gtattacggc ggcggcgtgg
     7201 acaacctgat catgcgtgtc acagatatct ttatgaccct gccgtatatc atgatggcgc
     7261 tgattttttc ggcggtgctg accccaattc tgggccgcag cctgctgccg cctctgatcg
     7321 ccatgatagc attcggctgg atgggctatg cccgcatcat ccgctcggat atcctctcca
     7381 tcaaagagcg cgactacgtc ctggcagcca gagcttccgg cctcaagaac ggccgcatcc
     7441 tcttcaaaca catcctccct aatgccatct tcccgaccct ggtgctggct tctctgggca
     7501 tcggcgatgt ggtgctcggc tttgccgccc tgtccttcct cggtatcggc accgaggtcg
     7561 gctacgccga ctgggggcaa atcctcagct tcgcccgcaa ctggattacc agcctggata
     7621 aatactggta catcgtggtc tatcctggca tcaccctgac cctgttcgtg atgggctgga
     7681 acctggtcgg cgatgcgatg cgcgacgtgc tggacccgcg tatgcgcggc aagggctaag
     7741 attttacgga aagcttacat cccctgagaa atctcagggg atgcttcttt aacattcagg
     7801 cgggttataa tctcttcata tgctgcgcaa agataaatcc ctcatcggct cctccaatat
     7861 cctcgggatt aacctggatg gcgagcgtct gctggttgtt tacggcagcc caaccggaac
     7921 ggtttacgag cgcctttccg ctcccatacc tgcgcaggcc aactttgcag ctgtgttgga
     7981 gctgattatg gtacaggccg accggctgct gaccctgaca caggcccagc acctggctct
     8041 gcccgagcgc gtctccgttg ccatttccgg caacctggac cccgaaacgg gcatccttag
     8101 ttccgccata gacttccccg cctggaaaca ggagccagtg cgctcccagc tctcgctgcg
     8161 cttcaacctg ccggtggcgt tggagcaaaa agcaaacgcg ggagcgctgg cggagtatta
     8221 ctttggcagc ggccaaaacg tccgaaacct ggtcttcctc agcatgtcgc ccaccctgcg
     8281 cgccggcatt ctcaccaacg gcaggctgta ccacaacagc ggggggacgg cgggccagct
     8341 tggcagcctg cgcctcgcgg cggagggccc ggcgggctcg tccaagcccg gcaccctctc
     8401 agattttgcc agcgcggcag gcatgctgga actggcgcgc aaacgctgcc cccaacactg
     8461 ggacagggat accaacctgt tccaggtgat ccgcgatgcc caggccggcg acccattcgc
     8521 gcaggatgtc ttcgctgagg ctggcgccca acttgggcgt gggctggcgg cgctggtaca
     8581 cctgctgcag ccggacctga ttatcgtcgg cttcccgggc tgcctgctgg aggagcattt
     8641 gctcgagcct gcccgtgccg cgctcgccgc tgcgacggag ctggccgaat ccgctctgcc
     8701 cgggctcatc ccttcggcgt tgtgcgcccg cctgcctgaa ttggaagcca tcgcgccagc
     8761 tatccaccaa ttccattcgc agaacaaaga ataaaaaagg tctgccaatt taggcagacc
     8821 tgaagtgcct gagccgtact catgcggtat gaggcatttc ctccacggaa tgcttctttt
     8881 tctttttctt cttctgtgcc tgagccatct cttcttcgag cctacgctgc ttttttccgg
     8941 cttttgtcat aagtttggcc tgttttttgg ccagcttttt ggcttctttt tccttgcgct
     9001 gggcaagccg ttcggcgtcg aacttttcca gcttaatgct gacccgcaca gtttccacca
     9061 gatagtaaac ataaagaatc aaggcaatga tgccaatcgt caaggtgccg taggtctggc
     9121 tgccgaataa cggcagttca acgttcttta ccccgatgaa gtaattcacc aggctgaaaa
     9181 gaatcgcaat caagcgcatt tccgtcgggc ctagtttaat ggtggtcatc tcaaacacgc
     9241 cggtcacttt cgtcttcagg aaaacggtaa tcatcatcaa caggtagccg atggcggcaa
     9301 agagcgtgac gtccaagcga gaaatgccgg aaaaaccaaa accgaggaac atgcacagcg
     9361 cggtaaaacc gtccaccgag tggtcgatga aatagccgta attcggccgt tccatgtggc
     9421 ggtagcgcgc cagcgtgccg tcaaggctgt cgccaaacca gttgatgaca aaaccaagac
     9481 aggcaatcag gacccagtag ttgcgcaggg gtccgccagt gcggccgaca atgatgaaac
     9541 tgaccagggt cagcagggag ccgaggatgc cgataaccgt aagcgtatcg gaatttaccc
     9601 aggcgggcat gtgcgctgcc aaccactgca gcgccggtcg ttccaaagaa ccaagcagga
     9661 tatcgttctt tcttacttct gtttgttgct tcatatgcac ctctcaggat aaatgcttaa
     9721 tttgctccag cgcttccggc cagatgggat agaacattgc ccattgttcc ggggaagcct
     9781 gaataaattt ctcagctgcg cgcagcactt tttctgcgtt cagcacaata tcatcggtca
     9841 ggttttcata gtcctcaaac gcgataggcg gggtgcagtc ataataaaac ctgccgtctt
     9901 cgcgcgttac cccacaggcg acccgtacgt cggctccggt ttcgcgcgcc aggcgcacgt
     9961 aatacgtgga aagcctggcg ggcctgccaa aaaataccgg tttgtatttt tgttcttctt
    10021 tcccatcaat cggccggtcc aatcccgtga ccagcgcgca gccttcattc agcatcagct
    10081 tttttgcttc cctgaatgcg ctgaaattaa tcggcataat ccgcatcccg gtctgctcgc
    10141 gcagagcgtt ttgcgcctga taggttttgt tgggtttcgg gtaggaaagc accaccacct
    10201 tctggcccaa tttcacaatc atcatgccga acaggtcaaa attgcccata tgcggcccaa
    10261 ggatgattag cggttttccg cgttgagcgc tgtcttccaa catcgactgc atttccgggg
    10321 agaggataat acactgccgg gcttcgtctt cgtgctggta ataataaaag tattcaaaca
    10381 aggcaataac gatattgcgc atgaccagcc gactgcgcgc agccaattcc gcgcgattca
    10441 gcctttcgcc ggaggctacc cattggttgg ctttgatggc ttgcaacatt ttcccgccct
    10501 ggaaggtgga aagaatatcc gcagcaaaga tggccagcct gcgtcccgcg cgcagcgagc
    10561 agtgcctccc aagccacatc cccagaccta tcggccctga atcctgaaag aaagctttaa
    10621 cagacatctt ctctacaatt ttaccggata acctcgaaag tgttttttgc gcccgacgct
    10681 tcctttcgca tcctgccgcg cgcgcgaagc tgccggaaag ccgccgcggc tgcgtacccc
    10741 aggctggcta ccaggataat caccgcgccg gaaggcaggt tgagcgcata ggaaatcaac
    10801 agccctgata tcgtgaagag caggcttaac cccgtgctca agaccataat gctgcgcatc
    10861 tcccgcacaa acagattggc aatagccggc gggatagtga gcaacgcaat caccaaaatc
    10921 aagccgacca cccgcatagt gatgaccacg gtcagcccga taagcacaat catggccatc
    10981 gaaagccagc cgacagggac gttccgcact gtggaaaaga cctcgtcgaa ggaaatcgcc
    11041 agcaggagcc ggtaaaacac cagcacaaaa gccagcacta ccacggcaat aaccagcatc
    11101 aagcgcaaat cacccgctga gacggccaga atgctgccga acaggtagct catcaggtcc
    11161 gctttatagc ccgggctgag gctgacgaag ataatcccca gcgccatgcc caacgcccac
    11221 atcaccccga tgatggtatc agcgcgcgct ttttccttgc ggttcacata gcccatggca
    11281 agcgaggaga gcaaactgaa acccaccgcg ccccacatag ggttcaagcc gaggaagtag
    11341 gcgatgccca cgccgccgta ggccgcatgc gcgatgccgc cgctgatgaa cacgatgcgg
    11401 ttcaacacca ccagcgtgcc aatcacgccg caagccgcgc tgacaatgac gcctgccagc
    11461 agcgcgttgc gcatgaactc gtacgaaagc acttctcgca gaaagttaat cattccagcg
    11521 gctctccgtc ctcagtatgc gcggggaaaa cgcggtgcgg catcccgtgc gcgattaagt
    11581 ccaccgggca ttcatagcct gcctggagca tgtccgcgga gatggattta tcgcggtgat
    11641 agaccagccg gcggttaata cagccgatgg tcttcacgta agcggatatc gcggtcatgt
    11701 catgggaaat cagcagaatc gagatttcct cgttcagcct ggccagtaat tcgtaaagct
    11761 gaacgctggc ctgcgggtcc acgctggcgg tcggctcgtc caacagcaaa atgcgcggct
    11821 gcgcggtgag ggcgcgggca atgtaaacgc gctgccgctg cccgccggaa atctcattga
    11881 tggagcgttt actcaggtca agcatgcccg tttggcgcag ggcttcctcc acgcgctggc
    11941 ggtcttcgct gctcaggcgc aggctggata agccgggctt aatcctgccc atgctgacca
    12001 cgtcccagac gttgattgga aaagctttat cgctgacttg cagctgagga acgtaaccga
    12061 tgtactttct gccctcctgc gggctggttc ccatcacgct gacgctgccg ctgcgcgggg
    12121 gcagcaagcc caaaatcacc ttaatgagcg tggtttttcc gcctccgttg gggccaatca
    12181 gaccgacata atcccgctgg tagaggtcaa agctgacatc ttccaaaaca gtatccaggt
    12241 cgtaccccgc ccagacattg cgcagcaaaa ttacaggggt ttcacttttt tcttccaaga
    12301 tatcctcgct accgaagcgc ttccgccaac tcggctgcca acaagcgcat attcgcagac
    12361 caatcggctg ccatcgggtc cagttccacc acgcgcgcgc cgccaagctg cccggcaatg
    12421 gactgcgcca gcttcgggct gatacctttc tggacaaaca cagttgtaat tccgttttcc
    12481 cgcgccaggt tcaggagcgc gcccagctcc tccgggccgg gttcctgccc atgggattca
    12541 acggaaagca tggtcagccc atattcactg gcaaagtagc cccaggcagg gtggataacc
    12601 agaaagctgc tgcggctgat gcccgctaaa gcggcgcgca catccgcgtc caccgcgtca
    12661 atttccttca gccaggcttc caggttggcg cggtagcttt gcgcgtgcgt cgggtcggca
    12721 gcggcaagcg cgtccgcgat gttccgcgaa ataatcttca cccgcgtagg cgaaaaccag
    12781 atatgcgggt cgttttcagc ttcctcagcg ctttctccaa gcgtagggct gattgccagc
    12841 cgttccaccc ctgcccctga atcgacaatc tccagcccgg ggttggcggc gctaaagcgc
    12901 ggcagccagg cttgctcaaa ctccacaccg atggaaaagt acaggcgcga ctttgtcagc
    12961 gcgcgcatct gttcggcggt aggctcataa gtgtgggggt catccccggt gcccacaacc
    13021 gcctgcacgg aaaccaggtc cccggcgatt ttttgcacaa agtaagcttg cggcagcacc
    13081 gaaacagtca ctgccatttg ctgcgcttgt tcaggtgcgg cgggcgagca ggcagaaagg
    13141 agcagcgccg cgcacaaaat gataattcca agtgttttca tttcgtttca ccggcttcca
    13201 gcaaggcctg gcactccggg cagaggccgt aaaactgcag gaggtgctcc cggaccacat
    13261 agcgggtttc gttttgaacg cgctgaacca gctcccgcat atcttcagag cccatgaact
    13321 ccacggcctt atgacagttc tggcacacga taaaatggtg atggccgaaa cttgccggca
    13381 ggtagcccac cagaccgtcc tgctggaata cctggctgat caatccaagc gagaagaaca
    13441 gttccagcgt gcggtatacg ctgaccaagc ccaggtgcac gccctgttcg cacaagagcc
    13501 ggtgaatggt aagcggattg agcggtcttt ccgccacttc catcagcgtc ataatccgtt
    13561 tgcgcggttc ggagagcctg tagcctgcct ttgagagaaa agtttgccag tccatcgcct
    13621 gttattgata aatattttca gtaagcctat tgtagactgc cccctgggaa ttgtcaaaca
    13681 gcagcactcg acaggcatac atactgatgg acagaccttg atattctgat ataatcatgc
    13741 ggctctggaa aatccaggcg tctttttaga attggagaag aaatgacaga aaatatcttg
    13801 aatgcggtgc aagacggcac cgttgtgcag ctggcctaca atctcgtcgt tgacggcgaa
    13861 gaaattgaat ccgaagtact tgagtacctg cacgggcata agaacatcat tcccggtttg
    13921 gaaaatgccc tgaccggcat ggaagtgggc gattccaaag aagttttagt cctggcccaa
    13981 gacgcttacg gcgaattcga ccccgacgcg gtggtgctgg ttacccgcga atccttcccc
    14041 ccggactttg aaatcctcct gggcgaaccg atgcacctgc gcgacgccga cgggcacgtt
    14101 ttcaccggag tggctacggc gctgaccgat gaaaacgtgg aattggatct gaaccacccc
    14161 atggcgggta aagacctgca gtttactgtg accatcctcg gcatccgtcc cgccaccgag
    14221 caggaaattc agaacggcgg tttgtcgcac tcgtgcgaaa gctgcggcga aggctgctct
    14281 gacggctgct gcggataaac tgctccaggc aaacttaaaa acgctgcgct tcagaggcgc
    14341 agcgtttttt gtatccagaa aagaaccctc agccggcagc ggccagctca caaattttga
    14401 tgatgacctg cgcggctttt tccagagagg aaaccggcac atattcgtag cgtccgtgca
    14461 tattatgccc gccggcaaac agattcggcg ttggcagccc catgtacgac agccggctgc
    14521 cgtccgtgcc gccccgcacc ggcacgacgc gcggctcaat ccccagggct tcgtaagcgg
    14581 ctcgcgccag tgctaatatc tgcggatgcg ggtcaatcat cttcttcatg ttgtagtact
    14641 ggtctttaag cgtcagctcc accaccgcct cgccgtattt ttggttaagg tagttcgcgg
    14701 cgctctgcat caaggctttg cgatcctgga aacgcctgtc gtcatggtcg cggataatgt
    14761 attccatgcg cgcggattcc acttccccat tcagcagcac caggtggaaa aagccttcag
    14821 tcttttcggt atattccggg cgctcaaaaa ccggcagcag cgcgttatat tccatcgcga
    14881 tttgcagcgc gttcagcatc acgcccttgg ctgtgcccgg gtgtacgttg cgcccgcgca
    14941 cctgcagcct ggcgctggca gcgttgaatg tctcgacctc cagctcgccg atttccccac
    15001 cgtccacagt ataagcgaag tccgcgccaa aagcccccac gtcaaagtgc tccacgccgg
    15061 cgcccacctc ctcatcgggc gtgaacgcga cccggatttt cccgtgcggg atttccggat
    15121 gggcggccag atacgccacg gcagtcataa tttcggcgat gccagccttg tcatccgctc
    15181 ccagcagggt tgtgccgtcg gtggtgacca ggctttcccc gcggtatttc agcaaatccg
    15241 ggaagcgctg cggggagagc acaatctgtt ccgccgcgtt caggataata tccccgccgt
    15301 cgtagttctc cacaatttgc gggcgcacat ccttcccgct gaaatccggg ctggtatcca
    15361 cgtgcgcgag gaagcctatc accggcgcgg gcgcgctggc agtggcttca agcgcggcgg
    15421 tcacgtaccc gaactggtcc atgcgtacgt cgctcaagcc aatactgcgc agctcttccg
    15481 ccagcagccg cagcaagtcc aactgcctgg ctgaactggg gtaagtctcc gagtcccggt
    15541 ctgattccgt atcaatctgg acgtaacgca aaaaacgttc caacactgca ggcatattgc
    15601 tccctccgtg gataactggc aaattatggg tggattttat cacgcaggta tcatccccgg
    15661 ggaattccca tgtataatcg aaattcaggc gcatccccgc atccgcgcca aaacatgttg
    15721 aaaccgccga acctatgaaa aaaacaaaaa acaccccaaa accaaagtct taccgcttgt
    15781 tcatcgcgct ggcagcgctg gcgctttttt tgctcgtgat gcatgtgcgc cttcccggtt
    15841 ccaactccat cccccggaag ctgggcgcaa acgcggagat caaggtgctt gcccaaaccc
    15901 cgcacgaccc gcaatccttc acacaagggc tgctctacgc ggacgggctg ttttacgaga
    15961 gcgccggttt gtacgggcag tccaccctgc gcaagctcga ggtggagagc ggcgcggtcc
    16021 tgttggaaga acgcccggat gaacagtact ttgccgaggg gctgacgctg ctgaacggca
    16081 agctttacca gctcacctgg aaggaaaaca agggcttcat ctacgacccg caggatttca
    16141 gcctccttgg cactttcacc tattccacgg aaggctgggg cttgaccacg gacggcagca
    16201 gtctgatact ctccgacggc agcgcggctc tgtatttcat cgacccgcaa agcatgctga
    16261 ccacccgtac tttgcaggtc actctggacg gcgcgccgct ggagcgcctg aacgaactgg
    16321 agtatatccg cggggaaatc tacgccaata tttggtacaa agacttgatt gtgcgcatca
    16381 accccgagag tggggaagtc atcggcgtga ttgactgcag catcctgcgg gaaggccaga
    16441 gcgcgccgct gcccaaagac gtcctgaacg gcatcgctta cgacgcgaaa gaagaccggc
    16501 tatttatcac cggcaaaaat tggccctgga tttatgaagt gtccttggag acagtgaaaa
    16561 cgccctgaag cttatgcgct gctgacccgc ccgcgcagta cgtccagccg ggcgttcatc
    16621 aaccccggca gcgtatccag cacgtaatac ggcccaaagc cttccatgca gacggcggcg
    16681 gtcacgtttc cacacagcgc ggcttccacc gggtcgtatt tcttgcggta gttcaacaga
    16741 aaagccccat caaaggcatc caacatgccg gtcgggtcaa ctgttttggc cgggtaagcg
    16801 ggaatgacgc ggcgtttctg ccccaggcgg tcaaacaaac gcaccgagcc gtcgggcaag
    16861 tgcaccaaaa caaattccgg tccatagtct gccagggctt cggcgatttc ccacaggtcc
    16921 acgctgcgcc cgcggaaaag ccgtaaagct tctgcttccg tgagcataaa agcggtcagg
    16981 tccgcaacca atccgcgcat ttcctcccag aagctggaat ccatgtaaca gccgcaggcc
    17041 cgcatggtga gcgtttcgac catgccggct ttcaataggg agggcagtat cttatgagag
    17101 aggaagtcta ttgggcacag gtgggcagcg ggggcgtcca ggtaagagcg aggaaggtcg
    17161 tttacccgcg cggtgaagtt ttcgtactcc agtttgctgc agaaagggga agacgcgctg
    17221 tcgtagccga taagctcgcg cggaaaagtg atgccccgtt ccgcgaagta ggtaatcggg
    17281 ttatcggtgt gtgggcttaa tgggtcggtg tacgcgacga agcgccggtc gtcaatgcgc
    17341 tcccgcacgc gcacgatgcc gttcaggtcg cagcccttct ggcgcagcgt atccagctgt
    17401 tccagcggaa agccttccgg aacacgcgcg tacaagcctg ccctgccgcc ccaaaaagcg
    17461 aaggcagccg cggcataagg caggctgccg ccaagcgcgt tcaggcgcgg cttgccctca
    17521 aagggcagaa taaaatcacg ttggagacgg cccccaatca cggctcccaa cggtgcggct
    17581 gggagttccg tcattgctgt tatttcgccg ccaccatctc gagcaggcgt ttgtggtaat
    17641 cctcgatgtt ttccaccggc gtggggtagc gcatatccag gtttttcaag gtcttgatca
    17701 caatcttgga gatcaaccag ttgcggtacc acttcttatt ggaagggata atgtaccagg
    17761 gcgcccaggc ggtgttggtc atgttcaaca tatcctcgta ggctttcatg taatcctccc
    17821 aacgggcgcg ctcatccaaa tcgccggggt tgaacttcca ttgcttggta gggtcttcca
    17881 cgcgcgccaa aaagcgctca gcctgttcct gcaaatcaat gtgcaggtaa aacttgagga
    17941 tggtggtgcc ttcctctgcg agcatgcgct caaactcccg aatatgctga tagcgtttgg
    18001 accagacatc ttccggcacc aggtcgtgga cgcgcactac caaaacgtcc tcgtattgcg
    18061 agcggttgaa aatgaccatc tcgcctttag cggggctttg ctggtgtacg cgccagaggt
    18121 aatcgtgcgc gagctctacc ggcgtcgggg ttttgaacga cgccactctg acgccctggg
    18181 ggttcacgcc ctcaaacacc gctcggatga cgccgtcttt gccgccggta tccatagcct
    18241 gcaaaataat cagcaggcgg tgcttgccct gcgcgtaaag ttcttcctgc agcgtttcca
    18301 gctcggcgtt taacttctgc aaagcttctt tgccgtcttt cttattccct tcgaacagtt
    18361 ttgaatcgtt ggggtcgtaa tccttcaact tgattttgcc tttaagcggg acacggtaca
    18421 tctcagcgtc catgattcac tccttatggc tcggttgacc gtcttaatta taccgggaat
    18481 ccgcaccctc ttccggcaaa tcgcttcttc agggcgtcag cgccaagttt ttcgctgcca
    18541 ggcgcataaa gcaagcaagc tgccttgcag cagcttgctt ttatccttgg gtacgggaga
    18601 cttacttacc ctggtccaca accctgatgg atttgggctt gatctcaatc cgtttgggct
    18661 gagcttcggg cttcttgggg aggtgaacgg tcagaattcc gttctcagtc tccgcgctaa
    18721 tcttgccctg ctctatctgc ccggggaggg agacactccg gtagaaggtg cccacccggc
    18781 gttcctgcac atggtatttg ccttcttttt cctctttgtt ttcctcccgg atttcacctt
    18841 taatactcag agtgttgtta tcgtaggtga tttccagcag ttcagggtca aagccggccg
    18901 cgtccgcccg caccaggaat tcgtcatcct tctcaatcac atccagcggg aagccaagcg
    18961 gctgggaacc ccaggcatca gcattcatat ccagaaaacg gtccataaac tgatccaggt
    19021 tgtaaagctc ccttggataa aatcgacgtc tcacaatatt tgccatagct acctcctttt
    19081 ttgtttgttg tttatgctta acttaagcgc tgattgttag attcctgtaa gcaattcata
    19141 aaaatttggt taattcacac ccgggatcct gctggaatcc tgcccaggtt ttgtaggtac
    19201 aaaggaataa tggccggaaa tagcaaaata gaaatgtcct aatgagggga tgaaatcgtc
    19261 aggatggttt ttcctcgaaa ctaagccctt tttttttctt cttttcacta ttgcttcacg
    19321 cttgccctga ccctgaccct tccccaacgg caaacggctc tattttgcct tggacgagcc
    19381 gccggggaag ggaaaaatgt ttgatagaac taaagatcaa cctccgtccc agcgcttgtt
    19441 cataaggagt aactcataaa attagtttgc tgggaaaggg aggaagaggc ggaaggttat
    19501 acttgcctta tgtgtcaggg tattctcatt cctttgtaaa ataaattgga tgtgccgaat
    19561 gatgatttca tagctcctga tcaggacatt tctattttgc tactttagga cattatcatg
    19621 ttgcttttcc acatactgca cggatttgac gaaacgcctg cgcgtatgct atactctcgc
    19681 aagttaatcc agccgcctgg cggctttttg ttgaagatac aacttatttt gaaacaaaat
    19741 tccccggctg acctgtattc gcggggtttt atttttaaaa ccaaactata acatttacgg
    19801 ctgttgccgt tggcactttt tattaaggaa agattatgaa agagaaaaaa ttacgcatta
    19861 ttcccctggg aggcctgggg gaagtcggga agaacatgac cgtctacgag tacggcggcg
    19921 aaatcctggt ggtggacgcc ggcatgatgt tcccggacaa cgacatgatc ggtattgatt
    19981 acatcatccc ggattttgaa tacattaaac agcgcgccga tcacgtgcgc gggattgtaa
    20041 tcacccacgg tcacgaggac cataccggag caatcggaca cctgctcgag agcgtggacg
    20101 cgccggttta cgccaccccg ctcacgctcg ggatgatagg cgccaaactg aaccgccgcg
    20161 gctttgaaca caaaaatgcc aggcttcaga cgctgaaggc cggcgagacc gtgcaaatcg
    20221 gccccttcaa ggtgcacgcc ttccacgtct gccattccgt tccggacggc atcggcctgg
    20281 ggattgaaag cccggcaggg ctggtggtcc ataccggcga ttacaagttc gaccacaccc
    20341 cggtggataa ctggccgaca gactacgcca agctggcaga attcgcgcag aagggcgttt
    20401 tggcgctgct gtcggattcc accaacgcgg agcgcccggg ctggacgcct tccgagaagg
    20461 tcatcgactc ggcgtttgac cgcgtgttcc gcgaagccaa ggggcgcatt atcgtcgcca
    20521 ccttcgcgtc gctcatatcg cgcatgcagc aggtcatcaa tgccgcgctg cgataccacc
    20581 gcaaagtcgc cttcgtgggc ctcagcatga ccgaaaacgt gaaaatcgcg cggcagctgg
    20641 ggtacatcga ctttgaggag gatgtggtgg tgccggtcga aaccgccctg cagatgccgg
    20701 acagccaggt agtgctgctc gttaccggtt cgcaaggcga gccaacctcc atcctcggca
    20761 ggcttgccaa cggcagcaac aaacgctttg atatcaaaga gggcgacacg gtcgtgctgt
    20821 cctcgcatcc catcccgggt aatgaagagc tggtttaccg cgccatcaac cgcctgctgg
    20881 aacagggcgc gcaggtcatc tacgaagcca tcgcgccggt gcacgtctcc gggcacgcca
    20941 gccaggaaga aatcaagctg atgctgcatc tgacccgccc gcgctacctg attcccattc
    21001 acggcgaact gcgcatgctg aaccagcatg cccgtctggg catggaggtt ggcatggacc
    21061 cgcgccagat tgccattgtg gagaacggcc gcgtgattga gttcgagcac ggcgaaatga
    21121 agctgggcga acgcatcccc ggcggctatg tgttcgtgga cggcacgggc gtgggcgatg
    21181 tggaccgctc cgtgatgcgc gaacgcgaaa tgctcagtca ggacggaatc gtgctcctga
    21241 atctgataaa agataagtcc aacctgaaag aggttgagat tatcagcagg ggcttcatgt
    21301 ccccgaccga atcgcaagac ctttttgagg aactgcgcca acgcatcgag aaactggcgc
    21361 gctccgcgaa cggaaacctg gagcgggacg tgctaaacct ggccaagtcg cacatttttg
    21421 atgagaccaa acgccgcccg gtggtgtttg tgaatatttc caaagcctga gagcttacag
    21481 gcgcggtcat catctgaccg cgcctttttt tgctctgcag ggggactgcg gacctcgcat
    21541 cctgaacctg acaccctgaa aaggtcgaat cgcgtggggt gcatcatatt catgcgggcg
    21601 aacatttcct caggcagtcc gcggtaagcg gcggtaaaat tggaagcatc acccagacag
    21661 agggagccca atgttccgca caactgctta ccgccacccc agcgaattcc ccatcctgct
    21721 gctcactata ttaatcagca ttgcagccgt tgttgtaacc gctttcctga cgcagtgcct
    21781 gagtctgtta gttatcgtgt ttatcctggg agcatccatt ttttccagcc gcgcgatgca
    21841 agcccaactg atgcgcaaag ctttccttgt ctcgccggag cgcagcccgg aactggcgag
    21901 cgtggtgcag ctgtgcaaaa aacgcctgca gccgggttcg ttggaagtgt atgtggtgcg
    21961 cgagaacgtg gtaaacgcgt acacattcgg cctcgcggag accaaaacta tcgtgttgta
    22021 tgagcccatg ctgcgcatca tgagcggcgg agagctggcc tttgtgcttg ggcacgagat
    22081 ggggcatgta tccctggggc atacctggct gaacaccatc gtcggcggtc tggcgggttc
    22141 cccctcgcct ctcggtctcg gttacattat gtacctggtc ttcctgcgct ggtctcgcgc
    22201 ctgtgagctt tcgtgcgacc gggcaggctt gctggcctgc ggcagcctga acatagcggt
    22261 ttccgccctg gtgcgtctgg ccgcgccgaa aatccagacc ccggaagagt ttgagcaggc
    22321 tctgaccatg attgactcgc aggatgacgt cttcgtgaac cggttggtgg agctgtttca
    22381 atcccaccca ctgattatca agcgcatcaa cgccctcaag gaatacgccg ccagcccgga
    22441 atacgcctcc ctgcaagcca acgtggataa gaacctgcaa gcctaaagct ccggttggca
    22501 tttatgaatg agtctgtcta aaaagaactg ttttagacag tccaaagtgg gagaatgttt
    22561 ctggttcagg cgtcatttcg atagcgcaaa tccgcaatcg cccataaaaa aggcacggat
    22621 tgggaaaaca aaagagctgt ccgacttttg ggacagcttc ttcggtctcg aaggactccg
    22681 cgttctcgac tgccttatgg cgtaaagaaa aaagctgcct attcggcagc ttttgtatag
    22741 ttgtttttta gcgcttcggt ggatggagaa agcgcagccg gctttacctc gtcaggcttg
    22801 ctttccgtag cgccttcagg cctggtgtgc gtgtggttct gccctgaagg ggagcggtgg
    22861 tcggtggcat aaaagccctt gcctttaaac acaatcccga ccggttgata gattttgcgc
    22921 agcgcgggtt cgttgcactc cgggcaaatc gtcagcggct cgtccgtaaa tttctgctgc
    22981 tggtcaaagc gtactccgca gttttcacaa tgataggtgt aaattggcat ttctttattg
    23041 ctcctgtctt tctaacaaac aagtatagta ccatagataa gcggattcaa gcaaatgcag
    23101 ggatttttga cacaactctt acatttgcgc gcgggatgag ccgtgtaatc ccgttggtat
    23161 aatggggata tgaaaatgaa acgactgttc ctgctgctcg gcacggcggt gcttttgggc
    23221 ggctgcgtgt tcagcatctc gcctgctccc accaatcttc cgcgccccac caacaccgcg
    23281 ctgccgcagc ctaccgagac gcccgctccg ccgacaaccg cacccacgct gacgcccagc
    23341 cccaccccca ccggcacccc ggtttactat caggtggacg cggacgatga tatgtttggg
    23401 gtcgcgctgc gctacggcat cagcctggaa gccctgatga ccgccaaccc gacggttatc
    23461 ccctacgcga tgggcgtggg tacgctgctg ctcattccca tcacgcaaac gccgccagcc
    23521 cccaccagca cgcccgcgca agccggccag caaacgcctg aacccggcgc gagcctgcaa
    23581 acggactgtt accgcgacct cgcaggcggt gtgctttgct tcgcgttggc gctgaacgac
    23641 acagaaaagg acctggaaaa cgtcagcgcg aaagtgacgc tgagcggcgt ggacggcgcg
    23701 tacacccgca cgcagtcagc cgtcctgccg ctgaatctgc tgaaagctgg tcagagcctg
    23761 cccctgctaa cctatttcca ggcgcccacc ccggaacagt tcagcgccag cgctgcgatt
    23821 gatttctccc tgcctgtccc ggaagatgac acgcgctaca tccccgcgga agcgacagac
    23881 ctggaagtga tttgcgcccg cggcaatttg tcctgcgcgg tgaacgggcg cgttgccctg
    23941 cagccggatt ccgcccaggc cggcgcgctg tgggtcctgg tggtagcata ttcccaggac
    24001 ggccgcgtgg tcggcatccg ccgctgggat gaccaatcct cacccatatc gctggatttt
    24061 tcggtactcc tgtacagcgc gggtccggag attgcgcgcg tggaagttac cgcggaagcc
    24121 agacctgccc aataaggagc cttatgctga gcgacttgct gccaaacctg ggaaagcaaa
    24181 cctatattat ggggattctc aacctgactg ccgacagctt ttccggcgac ggcctgctgc
    24241 agcaggaaaa ccccctgcgc gcggcagtcc gacaagcccg cggctttgta gcggacggag
    24301 cgcacatcct ggatatcggc gccgaatcca cccgcccggg cgcgcggcca gttccggcgg
    24361 agcaggagct tgaacgcctg ctgccggtac tccgtgcgct gcgcgaggag ctgcccgatg
    24421 ccgtcctctc cgtggatacc tacaaagccg ttgtggcgga agaagccctg cgagccggcg
    24481 cgcacatcat caacgatgtg tgggggctgc gcgccgaccc ccggatgggc gcggtggcgg
    24541 ccgcgggcgc agccagcgtg gtgctgatgc acaaccgctc gcacccgcag aacgcgcaaa
    24601 ttgacgtcag cctgggcggc aggtatgtag atgtttccta tggggacgtc tgtacggagg
    24661 tggcggaggg tttgcggcag tccgtccaaa tcgcaaggga agcaggcatt gacggcgggc
    24721 aaattctcct ggacccgggc atcggctttg gcaagacaac cgaacaaaat ctgcgcctgc
    24781 tgaaacagct tgaccgactg aaagcgttgg gctttccgct cttgttaggg atttcacgca
    24841 agtccttcat cggttacacg ctgaacctgc ccccggagga gcgtctggaa ggcagcctgg
    24901 ctgccaacgc ctatggggtt ttacacggcg cggacatcct gcgcgtacat gacgtgcgcc
    24961 agaccgcccg gctggcgcgc atgctggacg cgatccgctt tgcctgacgg caggctcaac
    25021 tctggtcgtc ttccgcgtct tcgcggataa tacgctcagc catctcctgc agcgcctcaa
    25081 tttccgcttc gtccgtgtgg ctttgcttcc agtaaagctt cagcagctcc tcgggcgtca
    25141 tgttttgggt ttccccgttc tcccccaggc gcgcccgcag gttgaatttc ggcatccggt
    25201 tgattttcaa ttccagcgca agccgcgccc gttcgcggat ttgggcatca tcaatcgtcg
    25261 cttcgatgtt atccgggtag ctgacgttca gacgcacaat cgcgccttcc agctcctcag
    25321 gctgcggaaa gaccgcaagg attttttcca tcgtgttctc gccggcgcgg atgctgacat
    25381 tgaaatcacg aaaggggcgc gtggcaagtt tgtgccaggt gtaatgcgtc tccccgcggg
    25441 aaacttcggc aatcacgaag cctttgtcat ctcccacctc cccaaaatcc acgcgttcga
    25501 tagacccggc ataaatcacg ggcgggtggc tgcccaggtt caggtcctga aagcggtgga
    25561 tgtgccccag ggcgacgtag tcaatgcgct catcgtgcac cagagcctgg gagagctgca
    25621 cgtcctttcc cagcttgata ttgcgttcct ccccgaggag cgccccgcgg atggaggcat
    25681 gcgccgcgag gataaaaggc agctccgggt cgcgctgcgc taatgcctgc tgaatcagga
    25741 cgttgaccat gttttcgatg attacatcca gttcagcctt cgggtctgcc gcttccgggg
    25801 cggagttttt tacaagggaa ggcgtaatcc agggaatgcc caacacctgc acaggcaagc
    25861 cttccagatc ttttgggcct aaaacctgtg gacggtcgat tacatgtacg tgcggaacgt
    25921 ccagcgtgga aaattcctgg atggcatgcg ctttttttga ggaaggcgag gtgtcgtggt
    25981 tgcctgtgat caggatggtg gggatgcccg cccgggaaag gcgcataatc cggctgcccc
    26041 atgcgcgctg ataggtaggc gccgggctgc ggtctttgta cgcgtcgcct gcgaagatga
    26101 ccagatcaac cttttcgtct attgcggccg cgacgatcgc gtccagggat ttgagaaagt
    26161 ccttcacacg gacaaacaaa gcctcatccg gatcgtaagg tccgtgcgtg gcaatatcaa
    26221 tatgggcgtc ggcaaagtgc agtattttga ccatatccca attatatcaa gccgggaaaa
    26281 acctcagggg gtaagcccca tgttgatcag ctcgctctct gccacccacc attccggatg
    26341 ccattcgagc gccttgcgga agtcgctttc ggctgccggc caattgccca gcatcttgtt
    26401 cgcgcgcccg cgccagaccc aggtttcggg gatggcgtct tcgggggtga tatccagcac
    26461 ataattcgcc aggttcagca cgtcgttata gcgggcggta taaaaatacg cgaagaacgg
    26521 gccggtctgg taccagaaca cacgccaggg cctgtctgac gtgggaattt cgtcgtatac
    26581 cgcgaaagcc tcgtcatatg cgtcagccgc gccggcgtaa tccttcagtt caatcaggat
    26641 gttaccgcgg ctgtaccagg caaagaacag ctcacggcct tccagcgtat cgatccgttc
    26701 gcttgtcttt tccaacgcgt agcgcaggtt gtagtcagca tcccactgcg gcccgaggat
    26761 gttgataacc tcctcttcgc gctcataggg atagaccaca aggtaaatgc cgtcaaactg
    26821 gttccaatac atgctcagca ggtcgtacgg cagggcgata tcccccagga acgaatcggg
    26881 aatgttcacc tttttcgtcg cgtcatcgta ggtggtaatg atgccgtaat gccccatcca
    26941 gccttccttg gcgttcatgt atccgcgttc caccaggaca gggaaacccg ccgcaaccag
    27001 ctttttgacc atatccacgt cgcctccgta gcgcacaatg cccgtcaggc tggtttgcgc
    27061 gttgacgtat tccagcattt cgtacggcat cacgttgcgg tcttctttca gaggcttgat
    27121 aaccgcttct accgtgtgtt ggtcgccctc ccagccccaa tacgagagca gcatggccag
    27181 gttggttggc ccgcagttat taaagcgctg acgctcatat ttgattccgg tcagagcttt
    27241 agagcgcggg atgggcgtgg aggttggcgt cggaggctgc gtcgccgtgg gggggaaggt
    27301 ggcctgttcc gtcgggtgcg gcgtggaggt gctgcttggc acgagcgtgg gggtgggcgt
    27361 gggcgccatg gcggtcagcg tggctgccac ttccggctgg agcgtgcctt tcccacccgg
    27421 gttgaaggct ttttctcccg ggggcttaaa aaagtattgg attctggcgt gtgccttcgc
    27481 gacgtaatag tatgcgtggt tttgtatcgc gggaatttgg tacagagcga tgagcagcag
    27541 cgtcagggct gccgccgctg ccagcgtaat ccaaagccag cgcgcgccgg ccttggcgct
    27601 gagcttgcgc tggatttttt tcttgttttt attagccata acaagctggc attatatcgc
    27661 aataagaata atccggtttt ggatggaaag cgcctggaag ggcaaaggaa tgctcatgcc
    27721 ctgacacaca aaggaggtgc agcctttccc cctcttcctc gccttcccag cgaacgaaat
    27781 ttattctatg ctcctgacga acgatcgccg ttgatctttg gggctgtccc ggaaaaagag
    27841 gctgccttaa ggcagcctct tgtatgcaaa tcaggcgatt ttacgcttcg tggaattcgc
    27901 cgtccacaac atcctcaccg ccttgcgccg ggccggtatt cggctggctt tccgcggctg
    27961 tcggaccgga ctgaggctgt tcgtacgcag cagcgccaat ggtctgcatc aggttctgaa
    28021 cagcctgggt ggccgagaga atcgctgagc tgttctcact ggtgaggctg tcgcgcagct
    28081 cctttatctt gccttccagc tcgccgcgct tgccggcgtc tgccttatcg cccaggtctc
    28141 taaggagttt ttcagcctgg taggcggtgc tgtcgccgat gtttctggcc tcaatcaagg
    28201 ctttgcgctt gtcatcctcc tctttgtgtt gggaggcttc gcgcaccatg cgttcgacat
    28261 cgttcttgtt caggttggtc gaagcggtga tggttacttt ttgttccttg ctggtggcct
    28321 tatccttggc ggtcacgctc agaatgccgt tcgcgtcgat atcaaacgtc acctccacct
    28381 gcgggatgcc gcgcggcgcc ggcgggatgc cgtccaggcg gaagcggccg agcgtcatgt
    28441 tgtccccggc cagcgggcgt tcgccctgca gcacgtggat atccaccgag gtctggctgt
    28501 cctcagccgt agaaaagact tcggttttct tgaccgggat ggtggtgttg cgctcaatca
    28561 gtttggtcat cacgccgccc agagtttcca cgcccaaaga cagtggggta acgtccagca
    28621 gcagcacgtc cttcacctcg ccgccaagca cgccgccctg aatggccgcg ccaatggaga
    28681 cgacttcgtc ggggttgacg cccttgttgg gctccttgcc gttggtcagc tggcgcacca
    28741 gctcctgcac catgggcatg cgcgtagcgc cgcctaccag cacgacttcg ttaatcttac
    28801 tgacgctcag gttggcgtct ttgatgaccg cgttgaaggg cagtttacag cgttgcagca
    28861 agtggtcggt catttgctcg aatttcgcac gggagagctt gagaagcagg tgtttcgggc
    28921 cggaagaatc cgccgtgatg tacggcaggt tgatttcggt ttccatcacg ctgctgagct
    28981 caattttggc cttttccgcg gcttcgcgca ggcgttggag cgcctggcgg tcgctgcgca
    29041 ggtcgatgcc ctgttcgcgt ttgaattcat ccgccgccca gttgacaatc acctcatccc
    29101 agtcgtcgcc gcccaggtgg gtatcaccgt tggtggcttt cacttccacc acgccgcccc
    29161 ccacttccag caggctgacg tcgaaggtgc cgccgcccag gtcaaagacc aggatgattt
    29221 cgttgtcctt tttgtccaat ccatatgcca gagctgacgc ggtgggttcg ttgataatgc
    29281 gcagaacctc aagccccgca atcttgcctg cgtctttggt cgcctggcgc tggctgtcgt
    29341 taaagtacgc cggcacagtg ataaccgcct gggtcacttt ctcacccagg tacgcttcgg
    29401 cgtcgctctt cagcttaccc aggatcattg ctgaaatctc ctggggcgtg taggtttgcc
    29461 cgtttacagg gatttcaatg cgcacgtcgc cgccgggtcc ggaaacgact ttgtaagaca
    29521 ccattttgcg ttcggaggca acctggtcgt agtggcgccc catgaaacgc ttgatggaat
    29581 acacggtatt ttctgggttc accactgcct ggcgtttggc agggatgccg accagacgtt
    29641 ccccattttt gttgaaagca accaccgagg gtaagaggcg cgagccttcc gaggtggata
    29701 ttacggttgg ggaaccgcct tccattacgg agacgacgct gttggtagtg ccaaggtcaa
    29761 ttccaataat tttgctcata aacaatctcc ttataatcta aattcacttg aatagactat
    29821 acctatccaa cgatagaagc gtattaacat tttcaatgaa ttatgtaaac ggctgccgaa
    29881 gttcggtcaa ggagtaaaaa atctaacagg gcaagcccgg gttagtttct cgcccgcagc
    29941 gccagcgaat ggccgaagcg atccatccag agatgcttcg cctgcggctc agcatgacag
    30001 gcctccggcg cggcgagccc gggcaggtct ctcacccgca gcgccagcga atggccgaag
    30061 cgatccatcc agagatgctt cgcctgcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30181 nnnnnnnnnc tcagcatgac aggcctccgg cgcggcaagc ccgggttggt ttctcgcccg
    30241 gggcgccagc aaagggccga accgcgatgt tgggtttcgc tcctgcgccg ctgccaccaa
    30301 ccgaagcgct gcagcccaac cgtgcaaagc tttcgcaaac gaagcgcttc aaaatctcgt
    30361 ataatctgta tggaaacagg caaaggaagt aatatgagca aaatgaaatc ttggatgacc
    30421 ggagcaatcc tgggcggtct tttgggcagc gcgctggtgc tgctctacac cccggccaaa
    30481 ggcagcgaac tgaaaagcaa actgcaagga gccgtgcaaa agatgcgcga cgaagtgcgc
    30541 ctggccggag aagaaaaacg cgccgaactg gaagcccagt tagacgcgct gcgttcaggc
    30601 gagtaaagcg cgctcacatc ccggctttta cgggtaatcc ggtaaaggcg tgggggtgtg
    30661 caccgggtac cccggcggtg tcgggctggg ataaattgac gtaggagtga tggtcggcat
    30721 caaagtcggc acaggcgtcg gggtgaccag caccacttcc gggtttggcg tagccagcaa
    30781 gtccccgata cccggcacct cgaccaccgg catattgctg atgtcgtcca catacgcaat
    30841 ccctgcctga caaaccggct ccaggtattg caatacgttc gcccagatga gcggctgttt
    30901 attggtcaca gtcagtcgga tggcccggga gaggcagccc gagcccgcgt tgtaattcag
    30961 cagggtgaat ttccacaagt cttcataggt cgaaagctga cccgcgctct taccggtcgc
    31021 attgtaaata atctgattga cctgctcgca gttagccaac agcccgcgcg cgaagacgct
    31081 gatgctgaaa ttcgcctggg tcaggtcaat ccccaccggg cagtccaggc agctcgcgtt
    31141 cactttttgc accagcgcgc cgcgcagcat ggcttgctgg gcctcgtcca ggttgccaaa
    31201 gccgcgctgg caggtctcct ctgccagcac cagcgggcag aattgcgtga agaactcggg
    31261 gttccagagc agcacggcgt ccgcgcccag ctcggtcatc tgccccagac cggcttcctg
    31321 cacccgggcg tagatgcccg gccagaactg gctttcccgg ctgaagatgt tcttcatcag
    31381 ttgggctggc acgccggttc cattggccac ctgcaaaatt tccgcgttga agcggttctg
    31441 ccattcattc acatacggac gcgcgagttc catcccacac acggtcgccg cgccgttgct
    31501 ttccaatccg ccgcgctcac aagcgctggc atccaccagc ccctgcttaa tcaaagagcc
    31561 ggccagcagg taatactcgt cgaacgaaac caggttttcg gagagcagcg gagtgttcaa
    31621 ccaggtcggc gcaccgtcca ccggcaaaaa cgcctgccag atattggagc agctggagag
    31681 attgtcctgc tgatactgcg aactgagcac atccacgtag tagaccggtt gatcggctga
    31741 agccgcctcc gggttggcaa actcgcccca gggaatcacg cgcacctgcg cggtaaactt
    31801 ttcgcttgcg tccccatagc tggactccgc ccaaaaaacc acttctacgc cgttcatgcc
    31861 ggtcggggcc agcggcaggt cgcaaatacc gccggggcag gtgaagggct gtccgttcag
    31921 ggtgcccaga atctggatga tttgctcgtt cggcaggggc tctacagctt ccatgcgcag
    31981 gaaaggcacg gagctgcatt ggttatcccc cggggcattg ccgcagccga cgatggatat
    32041 ccagacttca gccggcggaa gtttgacttc caccttacgg gtggagggtg tgacgttggc
    32101 aaggtgcaaa taaagcccgg ggcactgctg ggggttggta ataaggtcgt cgtatacgca
    32161 tgcggaagtt ctctgccact ggctgaggat cgtgctgccg caataatata aaacctcact
    32221 gtcatccggc caggattcgt gctccacgta aatctggcag accacctggg atgttttcca
    32281 ggagagcagc caccactcgt aggtggtgta atccacgtcc agcaccaccg agcgctgcgg
    32341 accttcagag gggagcggaa ctgaagcggc cggcttcggg ttcaggacaa gcagcacgaa
    32401 aacacagacc agaaagattc tgctcagaac tacgtttcgt gtgtttgttt ttgcgcccat
    32461 gtgcatcaga acaagtttgt tcctgtgtta cggatactca cccgaatccg tgggggtgtt
    32521 caccggataa ggggtcggga aaatggcggt tggtatcggg ctgcgggtaa cgccgggggt
    32581 tggagtaact ccgggcagcg gagtgcgcgt aggcgtcggc gtacctgtga taaccggaaa
    32641 gccgccagtc gctgttggcc ccagtggtgt cggcgaaaac gccgaagctt cggtcagctg
    32701 cgcttcgtag gtcagcacaa tctcccccag cgggctggat tgcaacacgt cttccagcgc
    32761 gcgcgcgaag accgcgttga ccgcctccgc gtccggcagg agcacgctgg tctgggtatt
    32821 atccggcagc tcccagctgc tgagttcgtt ccagccgagc agagaatagc tcaggcgctg
    32881 cgggtccccc aggcgcagaa aaagcgggat atccagcagt aattccggca gcgaaaaatc
    32941 ggtatccagc tggtctttgt acgaaacgta catcgccggc agcttcacca aacgtccatt
    33001 ctggactaat tttgtaaaga gcagctgcag catttgctgc tgccttctcg tccggtcgat
    33061 ttcatcctcg ccggaaagat acgaaacata gcaaagcgcc ttctgcccgt tcatgctgtg
    33121 caaaccggcc ggcaggccgc cgcacgcgtc gcggatgggc agcaaaacgc tgacatccac
    33181 cccgcccagg tcatccacca gccagccaaa ctcttctgga tgggcaacca catagcgatc
    33241 aggttttatc ccaaagttat acgcgagcgt ctcccgcaca aggttcatgc ctcccagggc
    33301 gtacgcggtg ttcaggcgct gcatggtgta gcccggaatg aacacgaaca ggctgcccgg
    33361 gatggaaatc acactggctt ttgagagccg cgtgttgaca aacgccagat gaaacgcatc
    33421 cgtgcgcccc cgatacgggg cttcctggtc ggagcccagg aacagccata catccacgtc
    33481 ctggggcagc gccatgcgct cggctgccgg cggaatcggc gtaacgggcg cgatgctggc
    33541 cggcgcgaag gtattccaga cctcgggggt gggcgtgtcc gaagccgcgg cagcggcgtc
    33601 cgtcggggtc gggaaagcca gcggcgtggg cgtgcccaca gcgcgcaggc agcccgtcag
    33661 ggcggcggtg agcgccaaaa gcgccagaaa gcacctcgcg taactcaagt attgggatgg
    33721 taaggtgcgc ggcgctctca tagcattaat gcgaaactac ggggcggttt cactccgctc
    33781 gtttgttgat tatagcacgg aatcccgggc tgcccgggcg agccagcctg catcgcgttc
    33841 ccttcggtca ctgtgcttag gtcagcactt tcgttaagcc ttgcatttat aatgccagaa
    33901 tcagcatcgc cgcggcgagc aaaagcgaga tcaggattga ccaggcccat aacaggtaaa
    33961 tcacagcctt ttccataccc ccgcgcaggg aaaagaacaa cccgatgccg caattcacca
    34021 gccaaatgaa aaggctgaaa aatgccagca ggaacacctg gttggagggc acctgctcca
    34081 gcgtcgtcca ggtgatttcc gcgcggctgc ccgccagcgc caaggccagc gccagcagcg
    34141 ccagccccgc cgccaagccg cccgccagca aaagccgggc agctttgtcc tgccagattt
    34201 tcttgcgcag gctgcccaaa ctttcggaac gcggctcgta attgaccagg ctgcccagtt
    34261 cgctcagacg ttcgtacaaa cttaaaaacg cttccggttc gcttggggaa atcgcgtaat
    34321 atcgccccga agacgccacc agaagcaaag cacgcgggtc cgccgcagca tactccacag
    34381 cgcccaggcc gggaaccgtg cgcgagccaa aaatcatacc cggcagcgcg cccggcggca
    34441 gcggcagcct ggtctggaaa tccgagactg gccgaatcca ctggacagcc ggcatcggaa
    34501 tgacctcccg gcgcaaaccc cagcgcagcg ccagcccacc gcgccccagg atatagcgcg
    34561 tagtgagcag cctgtacacc cttgaggcca gccccaccaa cagcccgacc agcagcacgc
    34621 ccgccagaat tctccatggc aggagcagct ggatgccgat accgcgcggg ttgaacacca
    34681 gcagaaacaa cagggccagg tcggcggcaa acaaaaccgc gctgacccac agcccaggcc
    34741 gtttggaggg gtaaaattcc gtttccatat acgcgcataa ttatacaggc gttaagcccg
    34801 ggcaaaaaaa actgcccggt ttttggccgg gcagcaggtc ttgttcaggc agcttagcgt
    34861 gctttgtatt ccgccccgcg tgccagcgcg cgggtgttca tatcgacaaa tttttgcagg
    34921 cgctgggctg tatgcgcctt gagcgcgcgc tccagcgccc cttccggcaa aatgtccatg
    34981 ttggccatca gcgccccgag catcaccatg ttggtggcgc gttcattgcc gatttccgcc
    35041 gcaatggcgt tggcaggcag cgccaccgcg cgcaggtctg tgcgcgttac ggcgcggttg
    35101 accatggatt ggttgacaat caggcagccg cctgcgacga ccagcggctc gtatttatcc
    35161 agcgagggca agttcatggc caacaccgcc ttcgggtact tcaccgtcgg ggagccaatc
    35221 tcctggtcgg agattaccac ggtgcagttg gcggttcccc cgcgcatttc cgggccgtaa
    35281 gacgggatcc aggtgacgtg tttgccttca tccatggcgg cgtaagccag cagctggccg
    35341 gcgtagagcg tgccctgacc gccaaagcct gaaatgatga tttcattctg catgtcagtt
    35401 ccctccctgt ttctcaaaag aaatcgcgtt cgagacctta aaatccccga ccgggtagta
    35461 gggcagcatg tgctcagcca gccaaacgcg cgcgtcggcg ggcgtcatgc cccagttcgt
    35521 caggcaggtg gaaagcagct cgaccatcga
//
LOCUS       DAOQAD010000009        32413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_1292_length_32413_cov_5.055010, whole genome shotgun sequence.
ACCESSION   DAOQAD010000009 DAOQAD010000000
VERSION     DAOQAD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 32413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32413
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1292_length_32413_cov_5.055010"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKL60_01580"
     CDS             complement(<1..625)
                     /locus_tag="PKL60_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNW94856.1"
                     /translation="MKIGFFQFSPRRGDVNANLSTISAALEDCEFDLIVLPELASTGY
                     LFDDPAELYELSEPGDGSGVTLSGLIELAARHRACIVSGFSERAPEGLYNSAAAVDGR
                     GVLCVYRKVHLFNTEKALFLPGNCGFPVVNYRNVHLGMMICFDWVFPEAARSLALKGA
                     QLICHPANLVLPWCQSAMRTRSLENAVFSITANRTGAESGQGQSLRFT"
     gene            685..1728
                     /gene="amrS"
                     /locus_tag="PKL60_01585"
     CDS             685..1728
                     /gene="amrS"
                     /locus_tag="PKL60_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNW94857.1"
                     /translation="MSKEFEALLYEKLPENKVRCQLCAHNCLIAEGKRGICKVRENRA
                     GVLYSLTYGRLIAAHVDPIEKKPLFHFYPGSRSFSVASPGCNFDCQWCQNWEISQGDS
                     TAAAARCPYTPAKDVVSSALHGGCRSISYTYTEPTVFFEYSQDVGRLAREAGLKNVYV
                     SNGYMSPETISLLRDWLDAANVDVKAFSDEVYRKHIGARLQPVLEACVRLKEAGVWLE
                     ITTLLIPGLNDDEAQLRGLTRFIARELGQDIPWHVSRYFPQYKYQDAEVTSLASIENA
                     LEIGKEEGLKYTYAGNVAGSADTLCPQCGATLIERSGMSVFSNRIKAPGLCPECGAVI
                     AGVGLAASEASLG"
     gene            complement(1720..3264)
                     /gene="hutH"
                     /locus_tag="PKL60_01590"
     CDS             complement(1720..3264)
                     /gene="hutH"
                     /locus_tag="PKL60_01590"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNW94858.1"
                     /translation="MTQIFLDGQSLTIEQVQHVASHPETRVQLSEACIPGIQKSANAV
                     AELAAKGEIAYGITTGFGAFKDRVIPPDQVETLQENILLSHAVGVGDFLNDETARAMM
                     LIRANTLARGYSGIRLETLQLLLALLNANIIPLIPEKGSLGASGDLAPLAHMALPLIG
                     KGRVRWQGREMPAADALAASGLAPVRLAAKEGLALTNGTTLMTAMGALLCAQTEVLLR
                     TSNLAAALTLEAQNGTLWAFHVDVHRLRPQPGQQETAALFRLLLQGSQFVRPHDPKNI
                     QDAYTLRCIPQVHGAVQHVLDQARETISIELNSVTDNPLVFQSEDGSPLIISGGNFHG
                     EPLAFCLDFLNIALTDLGNISERRIAKLVDSRCDSLTYPPFLTEHGGVNSGFMLLQYT
                     AAALSSENKTLSHPASTDSIPSSGNVEDHVSMGPNAALHCREIVKNLATIVAIELFCA
                     AQALDFRLQKQPEAQMGAVTRQAYALIRAEVPFIPQDSMLHPHLEKMLALVQAGKFAA
                     LTPDLA"
     gene            complement(3371..3769)
                     /locus_tag="PKL60_01595"
                     /pseudo
     CDS             complement(3371..3769)
                     /locus_tag="PKL60_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(3991..4317)
                     /locus_tag="PKL60_01600"
     CDS             complement(3991..4317)
                     /locus_tag="PKL60_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNW94859.1"
                     /translation="MAQLRRDYENFLAAGAQIMVIGPESPEAFTAYFTKEKLPFIGLP
                     DPTHTVLKLMGQQVNIFKLGRMPGQIIVDKSGLVRYIHYGHDMTDIPANEEILSLLDI
                     LNNESA"
     gene            complement(4336..4467)
                     /locus_tag="PKL60_01605"
     CDS             complement(4336..4467)
                     /locus_tag="PKL60_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94860.1"
                     /translation="MTLVTLNLPSPDFSLVDVHGNPVRLSDFKGVHHVILILNRGFT"
     gene            complement(4492..5319)
                     /locus_tag="PKL60_01610"
     CDS             complement(4492..5319)
                     /locus_tag="PKL60_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNW94861.1"
                     /translation="MNGRVKNLVPRYLLLVFFSFMVLLPISGLFLGAFKTDTQLIQGP
                     FSLPQTWNLDNFRQAWFTGRFNIFFKNSVIVSIAVVAASVFLSVLTGYAFGLLPLPGK
                     RWLFPLILLGYMVPFEAVIIPLYHFLDLLGLRDSYWALILPQIGLSVSFGTLWMTSFF
                     ENAPRDLVDAAAMDGCSRWQTLWRVLWPLAKPSVTTLVVLIFMWTWNEFLLALVLIQD
                     ETLRTLPVGLAFFQGKYTANIPLLAAGALIVALPTVIVYILFQRFFIRGMLGGAVKG"
     gene            complement(5316..6218)
                     /locus_tag="PKL60_01615"
     CDS             complement(5316..6218)
                     /locus_tag="PKL60_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNW94862.1"
                     /translation="MRANQAYPGENKWKGWLYLLPGLLIYLAFTFYPILETVRTSFYE
                     WNGFSAQRTWVGLQNYQMVFSDAQFTSAFLHNLVFILFYCVLPVFIGLLLASVLSRRA
                     LPGMTFFRTVLFLPQVISMVVVGVIWRWIFNPQFGPLNILLNALGLSGLTRAWLGDFA
                     LALPAVGSIGTWVQYGFCMILFLAGMQHISEEYYEASSLDGANAFQQFVHITIPGLRA
                     EIGVALVTTIIAALRVFDLVYVTTRGGPGNATLVVGFLIYRSAFQQNRIGYASAIATL
                     LMLMILAVSLLIRRLQTGNEEESA"
     gene            complement(6273..7592)
                     /locus_tag="PKL60_01620"
     CDS             complement(6273..7592)
                     /locus_tag="PKL60_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNW94863.1"
                     /translation="MKRIRFSVAILLVFAFILGACQGGSKPTETQAPAEETTLVVWDQ
                     FYRDVESQVMDTLNAEFEAAHPGVKIERVVKTLDDLKLTLKLALTEANGPDVAQVNQG
                     RSDMGAMVEAGLLLPLDSYLTKYNWSSVFSTSVASRNSFTPDGKTFGQGNLYGVSPTA
                     EVVGVYYNKTMFKKYGWEVPQTFEEFDALLAKIKESGETPISFGSLDGWNAIHEFSAI
                     QHLLVSLDYINNLTYGVNNVSFKTDENIKAARILQEWAKSGYFSEGYAGIGYDDSNAL
                     FKAGQGAMTITGSWLAGELINDTDQEFGFFLMPPFEGKTALAIGGVGIPFAIRSTTTK
                     ADLAAEYLNWMISPRAAELWAKAGMLPAMALPEGAEIETDNLFGDTLKAWDTINKGNA
                     VGHYIDWATPTFYDTLVAELQKLLGSISTPEDFVSAVQKDYEAYLNK"
     gene            8277..9278
                     /gene="glpX"
                     /locus_tag="PKL60_01625"
     CDS             8277..9278
                     /gene="glpX"
                     /locus_tag="PKL60_01625"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNW94864.1"
                     /translation="MNEAFSHLSWQLLKVTENAAIAAARSAGKGDKHLADEAAVDAMR
                     SALDEMRGFSSHIKIGEGERDDAPMLFIGERLGRGNIAIDIAVDPLENTNATADLKPG
                     AVCVLAASSSGGLIQASDTYLDKLCVGREAAPLVDIRAEPEENIRRIAAALHRENLAE
                     ITVTILDRPRNEELIRRVRSTGARVNLIMDGDLMPCIFTCFKGAGVHAVMGIGAAPEG
                     VISAAAIKLLGGGMQAVFKPKDDADRARLTRMGIRLDEVYTAETLAPGENLIFCATAV
                     TPILTTTKAVLEGVSFFPGGAKTHSLVVSNTFMNYVPTTHVLDSGKFSQEKSEFRLW"
     gene            9369..10439
                     /gene="gmd"
                     /locus_tag="PKL60_01630"
     CDS             9369..10439
                     /gene="gmd"
                     /locus_tag="PKL60_01630"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNW94865.1"
                     /translation="MKTALITGITGQDGSYLAELLLSEGYEVHGVIRRSSTFNTRRID
                     HLYHDPHGNGEKPRLFLHYGDMGATDTLLDVIYTTRPDEIYHLAAQSHVRVSFDMPEY
                     TGDITGLGTTRILEAIRKSKINSRFYQASSSELFGSARPPQNESTPFQPRSPYAAAKL
                     YAYWMTVNYREGYGMYSVNGILFNHESPRRGETFVTRKITRAVASIRAGLQNKLFLGN
                     LDAKRDWGYAPEYLEMMHLLLQQEQPDDYAIGTGEAHSVRDFLDEAFGYAGMDWHKYV
                     EIDPRYFRPTEVDYLLCDPTKAKTKLGWEPKIKFHELVRIMVDADLELLNQPKPGEGK
                     RIIDEKFNGWHRWEHQVVSMDK"
     gene            10719..13409
                     /locus_tag="PKL60_01635"
     CDS             10719..13409
                     /locus_tag="PKL60_01635"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNW94866.1"
                     /translation="MTELPKTYDFKEYEEKIYTEWESKGYFRPTNDPNKPGHDPSIKP
                     FVIVIPPPNVTGGLHLGHPLFVATEDLMIRYHRMKGEPTLWVPGTDHAGIATQLQVEK
                     QLAKEGLTRTQLGREAFEKRIWQWKEQYGGQITHQIRRIGASCDWERERFTLDDGLSR
                     AVREAFVRLYEKGLIYRGPRMINWAPGLKTAVSDLEVEYSEEMGTLYYFRYPLADGSG
                     DFIPVATTRPETILGDTAVAVHPEDPRYQHFIGRTVRVPVLGREIPVVADEYVEREFG
                     TGALKITPGHDPHDYEIGERHHLALINIMNPDATLNENAGPYQGMDRYTARKALWEEM
                     RALGLVIKEEPYMMSVPRSQRGGEIVEPLVSTQWFVRMGTLAQNAKAAVEAGGVTFVP
                     ERFTKVFHNWMDNIQDWCISRQLWWGHRIPVWYCQNCGEVIVARETPERCPKCGSEHL
                     EQDPDVLDTWFSSGLWPFSTLGWPEKTPDYEYFYPTSVLETGYDILFFWVARMIMDGV
                     EFTGKAPFHTVYLHGIIRDEKGQKMSKTKNNAIDPLEVMDSLGTDALRFTLLVGSTPG
                     NDMNISVKKVESNRNFANKVWNIGRFVISAVDRAGQRPAEEPVWTLPDLWIWARLKQV
                     VNSVNGLFENYQFGEAGKQVYEFFWGEFADWYLEASKRQLAEGGSRAYFTAFGLARVL
                     DVMLRLLHPFTPFVTEALWGYLKQACLDAGLPIHSRSGWEEALILARWPERMEMESWE
                     DEMIKDFNTTQELIRAIRNLRSDYKIEPARKLHAVFVSDKQQILLESQKLVLCDLAGL
                     DPELTEIRTEKPESQSGMAGLVVNEIEVLVQLADARDDAAEEERLRKELAEVESQIAR
                     LEALLASPFAQKAPEKIVQAEREKLETYRVSAEKLRMRLG"
     gene            complement(13594..15321)
                     /locus_tag="PKL60_01640"
     CDS             complement(13594..15321)
                     /locus_tag="PKL60_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94867.1"
                     /translation="MERQVPISSSEEIDLYLRTIYSLLRSTNVVQIRSLEEVHSGMNS
                     SLHPHAREPFPDTSALIYSLQRLPECIFHVKKVILGQTESSFSQHGYGNVESWTEVAA
                     RARRRRCFWDGGEQMACYIASRSDIDDVVPTLTALQIEWNKLNALLSHGPEELYRTAS
                     PSDPERFKRLALQLKLSEEELGRLYTVLGDRFNEVMGQFAQVKCNFRLQLLSGSLNDY
                     RKATETWWEKIEKQYPHINKRPIYFVSSNTHSVVNLLSGFALSRQDQLIDFIHSADQP
                     ALLAEWEDIQSKAVPSSQENFFYYVMKKYQSSSAGKDAWQEQLSFEKQRGIYRFPSVH
                     SFDIEAQVIELSRLNPESVDSRITPCPLPGCAEWSFLKKSDALIVNIDYPLGFGAYHL
                     LTKIAENAAQILGIYVMGKAASLNGVRGDVVLPNVVYDEHSKNTYIFDNNFQASDVSP
                     YLVYGTVLDNQKAVSVWGTFLQNATVMDVIYREGYTDIEMEAGPYLSAIYELFRPQRH
                     PVNEIVNLHKLPFDLGILHYVSDTPLTKGQNLGAGALSYLGMDSTYASSLAILRRIMQ
                     RESERVSAR"
     gene            15427..16038
                     /locus_tag="PKL60_01645"
     CDS             15427..16038
                     /locus_tag="PKL60_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNW94868.1"
                     /translation="MPIDTVIFDFGGVILRYLDPQWLRENMPYGAEIQEAIYEYLVSD
                     VLWEVMRGRLKEMDFWTRKAREKGIPVQAVQSYLQAQNARAQLDQQVLHHMARLKGRY
                     RLGILSNASDTTRALLEREYRLHELVDDIVISAEEGLVKPEPEIYLLAAQRLGTQPER
                     CVFVDDLPENIQTAEKLGMLGVLFKDTANSLARLDALLKQGGA"
     gene            16038..16730
                     /gene="rsmI"
                     /locus_tag="PKL60_01650"
     CDS             16038..16730
                     /gene="rsmI"
                     /locus_tag="PKL60_01650"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNW94869.1"
                     /translation="MLYLVATPIGNLGDMSYRAVETLQLVDLLASEDTRKTSILLKHY
                     GISKPQVAFHTMNEAKMTPKLLERLLAGENIALVTNAGTPGISDPGYSLTHAAVQAGV
                     PVTSIPGASAVITALTLADLPLHSFTFKGFPPHKEGPRKRFLEQEKDSPHTLVFYESP
                     YRLNAFLKNALEVFGDRRAVIANDLTKKFETIWRGNLSELQETIQNTAIKGEFCICIE
                     GRGEEKPKTPEG"
     gene            complement(16711..17445)
                     /locus_tag="PKL60_01655"
     CDS             complement(16711..17445)
                     /locus_tag="PKL60_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNW94870.1"
                     /translation="MNRTTPPSPPARTYRYLDIVTVVFTLVLALSNIASSAKLIDWGI
                     SLGGVPLAFDAGTLFFPVAYIFGDILTEVYGYKTSRRVIWIGFLGLAFTALMFLLIQR
                     LPGESTWLNTVGQTSYDKILGGVSSGGIVLASFLGYLSGSFSNAVIMAILKHTTRGKW
                     LWLRTISSTIVGELVDTAVFIAVGSLTRVFPWELYLTLTLTNYLFKVGIEVIMTPATY
                     WVTSRLKKAENLDTYSDLSDLTPLAF"
     gene            17490..18242
                     /locus_tag="PKL60_01660"
     CDS             17490..18242
                     /locus_tag="PKL60_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNW94871.1"
                     /translation="MQRFFVDPEEIQNGLVRFPEGAARQIEKVLRLDLHNGEVQVLDN
                     SGRSYRVRLRGRHAGALYGEIVQVLEADPRVELRLELGFSLTKREKVEWILQKGTELG
                     VSAFRPFVSERSLDRSLRLDPAKQERWRAILREAAEQSQRTRLPSLFAPRNFGEGLGD
                     FPRESVRLIAWEGSKPELQLKPDWFGKSKGQPGMGASLLIGPEGGFSAEEVGLAEREG
                     FRQFSLGRNVLRMETACLAACVLCAHYLDNAL"
     gene            complement(18239..19711)
                     /locus_tag="PKL60_01665"
     CDS             complement(18239..19711)
                     /locus_tag="PKL60_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94872.1"
                     /translation="MTSLVNDLTLSAVERYKDCLSSEDYAQLLKVISQPADIAVRVNL
                     LKSANPQAAFSRWSGRYGWQAKPIPFAPDAWQISSAQNPPGQTIEHRFGYYYIQDAAS
                     ILPVALFSALPKDGLTLDMAASPGGKSTQLVESADDASLLLANDSSASRLQALRVVLL
                     TWGAANSAVTNFPGERLGAWFPETFDRVLLDAPCSMEGLRVSASHPFRAITPGERERL
                     AARQYALLESAVSAARIGGEIVYSTCTLAPEEDEAVLSAFLEKHPGSARLLPSPLAGL
                     NAPGLTRFEGREFDHQTARSLRLWPHLAGTNGFFAAKLLKTAPLDLPRQTPPSRPFGK
                     TGLEPLDAKDQTALFASLLDLYSFDFRAEAEKRQLLLYRRDQQLSLLPEQYLRRFSDL
                     PYYAFGFPFGKWSGANFDLSPEMISRYGDRFGSNVWVIPEELARQWLQGSDIRGVEVS
                     GCRKSSIIALRDAEGRNLGAGKNLPGRLRNLLPSRAILTP"
     gene            complement(19711..20529)
                     /locus_tag="PKL60_01670"
     CDS             complement(19711..20529)
                     /locus_tag="PKL60_01670"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94873.1"
                     /translation="MDESLNAKIIAEVRTSRKYRELDLPENMLRQLLAEQAAKGGSAA
                     EITAGFRKKLHNIVAPYLELIDYPAETEKMLAFFGANPSEAQEKQWAAGVMARHASTR
                     ERLDHLDGFCGVLREIIGSPHTVLDLACALDPLMLLWLNLPETVNFHAYDIHKPRLDF
                     LSQFFALRFPNARAIHQDILTEPPELAADCAFFFKEAHRFEKRQPGCNGAFFAALRAP
                     LLLVSLPAADLSGHHSLADYHTQLINKACDGRGWEVERRQVADELLFVIRKGEM"
     gene            complement(20535..21347)
                     /locus_tag="PKL60_01675"
     CDS             complement(20535..21347)
                     /locus_tag="PKL60_01675"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94874.1"
                     /translation="MSPYSPEELETLIQAIRASEKYAQLTPSLVARVAAEELEKRGES
                     KSALKAARTRLHQITGAFLAPDINYAKWTDRLKVLPPENAEELQVFCRRMMRLHNSTA
                     ERLPYIEQFFTTCLADISPIQSVLDLACGLNPLALPWMPLSANGTYSACDVVLPMLTF
                     LNQFFSTVGIHAEAFPCDLSACVPKQKVQLAILLKTLPCLEQLDKSLAPRLLAELNAE
                     HILVSYPAKSLGGREKGMRLTYAQQFARLIEGTAFRVRAFDFPNEMVFLLSR"
     gene            complement(21328..22422)
                     /gene="tgt"
                     /locus_tag="PKL60_01680"
     CDS             complement(21328..22422)
                     /gene="tgt"
                     /locus_tag="PKL60_01680"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNW94875.1"
                     /translation="MSTLQVRGRALPLPAFLPDATYGYVRSLTSADLREVGVQAVMMN
                     AFHLMQKPGSSVIQSLGGLHNLAAWDGLIMTDSGGFQAYSLIRQNAKFGSLGENGIIF
                     RPESSQRKLILTPEKSIQLQFGYGADILICLDDCTHVDAPLAEQQLSVSRTIQWAKRA
                     KAAFQSQLSSRKLDLEHKPLIFGVIQGGGYPELRKTCAEALLEIGFDGYGFGGWPLDD
                     QSNLLTDILEYTRSVVPREFPLHALGIGHPVSVAASYRLGYEMFDCAMPTRDARHGRL
                     YTLSSSRAIPGRGQDWFAFHYINDEKYIRSQEPVSPGCDCPACQHYSLAYLSHLYRMQ
                     DSAYFRLATQHNLRFMTRLMEQLRDEPLLP"
     gene            complement(22419..23126)
                     /gene="rsmI"
                     /locus_tag="PKL60_01685"
     CDS             complement(22419..23126)
                     /gene="rsmI"
                     /locus_tag="PKL60_01685"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNW94876.1"
                     /translation="MTAKPAAGKLYVVATPIGNSADITRRAVELLSNVDAVVCEERKN
                     GARLLKELGLEKPLIELNEHNESSLIQEVLVLLMQGQSLALISDCGTPVFSDPGRQLL
                     ILLDEMKVQVVPIPGASSLIAAMSVCPFNLEQFQFAGFLPPKTEQRQSALTRLSRADQ
                     PIILMDTPYRLGKLLLEVQQAFGKKQRVFLACNLTLPGEKLYNGTVEEIIRQANNRKA
                     EFILILDRPQRKQFGGP"
     gene            23342..24883
                     /gene="murJ"
                     /locus_tag="PKL60_01690"
     CDS             23342..24883
                     /gene="murJ"
                     /locus_tag="PKL60_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNW94877.1"
                     /translation="MKKISRLTKVSLLLAVLFSLDKLLGVARQMLVSRQFGLSAELDV
                     FNAANNLPDMLFMLISGGALAIAFIPVLTEVLTKDGREKAWALFSNILNLAFLITAVL
                     ALLIAVFARPLVSGNLGIAPGFSGTQIALTTKLLRLNLIATLVFSLSGLVMAGLQANQ
                     HFLLPALAPIFYDFGQILGVLILAPSESYQIGPLRLPALGLGIEGMVYGVILGAVLHL
                     LIQIPGLKKYHFRWQPRADLKDPETRKVLRLMAPRMLSMLFIQLIFLAQDNFASRLAT
                     GSVTALTYGWWIMQVPETLIGTAIATAILPTLAELFSQQAFPQLKTKVERAGQVMLAL
                     TIPIAVIAGLVIQPLIQLLLGLGPADTARVVLVTRVFLLGISGHALVELFVRSFYAMQ
                     KPKVPLMLSAFTLLVYLGSGLLLSARYEAAGIAAANTLAYTLQALVLFILLSKVLAEK
                     LELLPTLARAVIAALLGGAAAWLVMRFAPIPGGALAASIAAGLLGLLPAALVLRKELR
                     ALTSL"
     gene            24886..26034
                     /locus_tag="PKL60_01695"
     CDS             24886..26034
                     /locus_tag="PKL60_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94878.1"
                     /translation="MEYNFLIMDENNENNTQEAPLAAEQETPIAAAEFDTAAETEAEG
                     APVRKRRVPWIWLGVLFLILMLALGTFLGYRKGIARRLAREQTTVVEVAAEQYQMAFQ
                     DMAAGNYQNAKARIEYVIRIYPQFPGAPELLRSILMNIEQPKPTPTLAAIAQLTPIPS
                     GTPDTRSAEEAFNAIAAAAAAKNWQAVVDNVIAIQQINYDYRTVDVAGYYYIALRNLG
                     IQKIQAGELEQGLFDLSSAEQIGPLDGEAEGVRSWAALYLSGASYWDVNWQRAIEIFQ
                     QIKEAYPYMMDSSGMTAIERYRVALYRYGDQIAAAGDYCTAYQYYLRSLAVAQDPNVQ
                     QTADLYKQNCESSRPTEVTPTEPTPPDETLVPPTEVTPSPPPTDNPTP"
     gene            complement(26105..27457)
                     /gene="rimO"
                     /locus_tag="PKL60_01700"
     CDS             complement(26105..27457)
                     /gene="rimO"
                     /locus_tag="PKL60_01700"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNW94879.1"
                     /translation="MKIEPGTFSISTMGCAKNLVDSSSMEHLLINQASLTCVEASRAE
                     FLIVNTCAFIHDAREESIRELEAFARRKKRGQKLIAAGCLSQRYQQKLLEQVPGIDGM
                     VGTRDLGDILPMLARLSAKTVPQARVSIPEHERIRFISGLPGYTVQGRSSYLKIADGC
                     RRSCAFCAIPGIKGSLVSRQKEDILSDALALQEAGIQEINLIAQDVTDYAQDLGIQDG
                     LPDLLEALLPAIPRVPWVRLLYTFPGFISGRLIDLMASSRQLLPYLDIPLQHADPEVL
                     RAMRRPSDVDWVRRTVTEMRARIPALVLRTTLLVGFPNETEQRFSALLNFLREMQFDH
                     VGVFTYSPEEGTPAEALGDPIPQAVKESHREELMTLQAGISMRKNQQLKNKVFSMLVE
                     GTDPEHNIVVGRTYRDAPEIDGLMIAEGKAQVGEMLSVRVKAAMQHDLYGEVVPTSAA
                     "
     gene            complement(27454..28767)
                     /locus_tag="PKL60_01705"
     CDS             complement(27454..28767)
                     /locus_tag="PKL60_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNW94880.1"
                     /translation="MSDLTGSQLKSIREERRIPLEQVANATRIRLALLEALEADEYAE
                     LGSRTQARGFLKLYADFLGVPLEVQAENSAPDLPQPAASQADAQTPTQPASPAEPAET
                     APQPAAPAKSPKARHVRIPRPGKAAKQPVEQPPVLPISTTRSQQILDDIGRELQARRK
                     YLDIPWEILVEHTHIPRKQLVALEQGLLDSFASLSEAKGLLQTYARFLNLDTDLLMLR
                     FADALQERRLEKAAPESKKRKTARRLPPFLLALRRFFTLDLFFGTILVGGILIFLLWG
                     AADLLNRPDTRVNATQLPEMMEVLIGSQTPAAIMPTPTPEITEQVVLLPSPTPLILPT
                     DTTASVQVVVQARQTVWVRVSADGEEVFRGRMMAGSAAAYSADEYIELEAGNIAALSF
                     VFNGAALEPINRVGEVGRLRFDPTGMTDLSSMPTYLLTPSPTPKP"
     gene            complement(28845..29387)
                     /gene="rplI"
                     /locus_tag="PKL60_01710"
     CDS             complement(28845..29387)
                     /gene="rplI"
                     /locus_tag="PKL60_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNW94881.1"
                     /translation="MKVLLIKDVYKLGRAGDIKKVADGYGRNYLIPQGLALPATENSI
                     KLAESIGHKASEKRAVLNNELKGVAEILSGLKLEFPVKASETGKLYGSVSSQMVADRV
                     KELKGIELDRHQIVLEPIRTLGEYEVPVHLTIDLVPLLQVIVHREGETVKARPAKAVE
                     AAAPAVEEAAAVEAKEDAEA"
     gene            29566..31221
                     /locus_tag="PKL60_01715"
     CDS             29566..31221
                     /locus_tag="PKL60_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNW94882.1"
                     /translation="MTDQESFEKWRTELYEKSVSKYPERKASFKTSSEIPLHPIVPPE
                     DIHPERFSELGFPGQYPFTRGVQPTMYRGRFWTMRQYAGYSSAEESNARYRFLLEQGQ
                     TGLSVAFDLPTQIGYDADDPIALGEVGKVGVSISSIEDMETLFREIPLDKVSTSMTIN
                     APAAILLAAYIAVAKKQGVDASKLRGTIQNDILKEYIARGTYIFPPAPSMRLITDTMR
                     YCREEVPNWNTISISGYHIREAGSTAAQEVGFTLANAVAYIEAAQAAGMDVDEFAPQL
                     SFFFNAHNNFLEEIAKFRAARRLYARIMKERFHAQKAESQKLRFHTQTAGSTLTAQQP
                     ENNVVRVTLQALAAVLGGTQSLHTNSMDEALWLPTEKSVQVALRTQQIIAYESGVADT
                     IDPLAGSYLIEYLTDQIETQAAEYIRKIDELGGALKAIEYGFVSNEIQDAAFSAQRRL
                     ETGEDVVVGVNKFQQHETLELESLRIDPVIEAQQRERLAKLRRSRDPQRTAELLSQLE
                     QAAKGSENLMPLFIECVESKLTLGEICNLLRSVWGEYRAPNFI"
     gene            31230..31634
                     /gene="mce"
                     /locus_tag="PKL60_01720"
     CDS             31230..31634
                     /gene="mce"
                     /locus_tag="PKL60_01720"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNW94883.1"
                     /translation="MKPIKINHVAIVVNDIDASLDFWVKAFGLQLDHIEDVPSQASKV
                     AFLPLGESEVELVQPTSTDSGMAAYLEKRGEGMHHLCIEVEDIEAALAHLKTLEVRLI
                     NSEPQVLPGRKMAFIHPKAANGVLIELYQLTD"
     gene            31717..>32413
                     /locus_tag="PKL60_01725"
     CDS             31717..>32413
                     /locus_tag="PKL60_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNW94884.1"
                     /translation="MLITNGKIVIWGDKPEIVEGQALHLQDGLIESLADTNALVQAHP
                     EEEILDAGGQLVMAGNICAHTHFYGAFSRGMAVPGEPPADFSAILSSLWWKLDKALDE
                     EGVRYSALVCLVDAIKYGTTTLFDHHASPNCIDGSLDIIADALRQAGLRASLCYEVTD
                     RDGEERALAGIRENARFIDKVARGAESPLIRAHFGLHASLTVSAETLERCLAANPHQA
                     GFHVHAAEGLVDQE"
BASE COUNT         7081 a         8745 c         9813 g         6774 t
ORIGIN      
        1 ccgtaaaacg caaggactgc ccctgtccgc tttccgcgcc ggtgcggttg gcggtgatac
       61 tgaaaacggc attttccagg ctgcgggtgc gcatggcgct ctggcaccag ggtagcacca
      121 ggttggcggg gtggcagatt aattgcgcgc ctttcagtgc caggctgcgg gctgcttccg
      181 ggaaaaccca gtcaaaacaa atcatcatcc ccaggtggac attgcggtaa ttcaccaccg
      241 ggaagccgca attaccaggg aggaacagcg ctttttcagt gttgaacaaa tgcactttcc
      301 ggtatacgca cagcacaccc ctcccgtcaa ccgcggcggc cgaattgtac aagccctccg
      361 gagcgcgttc gctgaagccg ctgacgatgc aggcgcgatg ccgcgcggca agttctatca
      421 agccgctcag ggttacgccg ctcccatccc cgggctcgct aagctcgtac agttccgccg
      481 ggtcatcaaa gagatagccg gtagaggcaa gttccggcag gacaatcagg tcaaattcac
      541 agtcctccag cgccgcgctg atggtgctga ggtttgcgtt cacgtccccg cggcgggggc
      601 tgaattggaa aaagccgatt ttcatgctcg cctccagtgt acaatacaaa tatctgtacc
      661 aagtgtacag ggaaggataa gctcatgtca aaggaatttg aagcgctgtt atatgaaaag
      721 ctgccggaaa acaaggtgcg ctgccaactt tgcgcgcata attgtctgat tgctgaaggc
      781 aagcgcggca tctgcaaggt gcgcgaaaac agggcagggg tgctttattc gcttacttac
      841 ggaagattaa ttgccgcgca cgtggacccg atcgagaaga aaccgctgtt tcacttttac
      901 ccgggcagcc gcagcttttc ggtcgcgtcg cccggatgca actttgactg ccagtggtgc
      961 caaaactggg aaatctcgca gggcgacagc acggctgctg ctgcgcgctg cccctataca
     1021 cccgcgaagg acgtggtcag ttccgcgctt cacggcggct gccgcagtat ctcctatacc
     1081 tataccgaac cgaccgtctt cttcgagtac agccaggatg tcggccggct ggcccgtgag
     1141 gcaggcttga aaaacgtgta tgttagcaac ggctatatga gcccggagac gatttccttg
     1201 ctcagggatt ggctggacgc cgcgaatgtg gatgttaagg cattctcgga tgaagtttat
     1261 cggaagcata ttggcgcgcg cttgcagccg gtgctggaag cctgtgtgcg cctaaaagaa
     1321 gcgggggtct ggctggaaat cacgacgctt ttaatcccgg gtttgaatga tgatgaagca
     1381 caactgcgcg gcctgacccg gttcattgcg cgtgagctcg gccaggacat cccctggcac
     1441 gtcagccgct acttcccaca gtacaaatac caggacgctg aggttaccag cctggcgagc
     1501 atagaaaacg ctctggaaat cggtaaagag gaaggcctga aatataccta cgccggaaac
     1561 gtggccggtt cggcggatac gctctgcccg cagtgcgggg caaccctaat agaacgcagc
     1621 ggcatgagcg tcttcagtaa tcgcatcaaa gcgccgggct tgtgcccgga atgcggcgcc
     1681 gtgatagccg gcgtgggttt ggctgcttcg gaagcaagct taggctaaat ccggcgtcag
     1741 cgcggcaaat ttccccgcct gcacgagcgc gagcatcttt tccagatgcg gatgcagcat
     1801 gctgtcctgc gggataaagg ggacctccgc gcggattaac gcgtaagcct ggcgggtgac
     1861 cgctcccatc tgtgcttccg gctgcttttg gaggcggaaa tccagcgcct gggcggcgca
     1921 aaagagctca atcgccacga tggttgcaag gttctttaca atttcgcggc agtgcagcgc
     1981 ggcattggga cccatggaga catggtcctc aacattgccg gaggacggga tgctgtcggt
     2041 gctggcgggg tgggaaagcg tcttattctc cgagctgagc gccgcggcag tgtattgcag
     2101 cagcatgaag ccggaattca caccgccgtg ctcggtcagg aagggcgggt aggtgagtga
     2161 gtcgcagcgg ctgtccacca gtttggcaat gcgccgttcg gaaatgttgc ccaggtctgt
     2221 cagggcgatg ttcaagaagt ccagacaaaa cgccagcggt tcgccgtgga agttcccgcc
     2281 ggaaatgata agcgggctgc cgtcctcgga ctggaaaacc aggggattat ccgttacgga
     2341 gttcagctcg atgctgatcg tctcgcgggc ctggtcgagc acgtgctgga ccgccccgtg
     2401 cacctgcgga atgcagcgca gggtatacgc atcctgaatg tttttggggt cgtgtggacg
     2461 gacgaactgg ctgccctgaa gaagcaaacg aaagagtgcc gcggtttcct gctggcccgg
     2521 ctgcgggcgc aagcgatgga cgtccacgtg aaaggcccac aaagtgccgt tttgggcttc
     2581 gagcgtcaga gctgcggcga ggttggatgt gcgtaaaagg acttcggttt gcgcgcagag
     2641 cagggcgccc atggcggtca tcagggtggt gccgttggta agcgccaggc cttctttggc
     2701 ggccagacgc acaggcgcaa gccctgacgc ggccagtgca tccgctgcag gcatttcccg
     2761 gccttgccag cgtacgcggc ctttgccaat caggggcagt gccatgtgtg ccaggggagc
     2821 caggtcgccg ctggcgccca acgaaccttt ttccgggatg agcgggatga tgttggcgtt
     2881 caataatgcc agcagaagct ggagggtttc cagacggatg ccggaatagc cgcgggcgag
     2941 cgtgttggcg cggatgagca tcatggcgcg cgctgtctcg tcgttcagga aatcgccgac
     3001 tccgaccgca tggctgagga ggatgttttc ctgcagggtt tccacctggt ctggcgggat
     3061 aacgcggtcc ttgaaggcgc cgaagccggt cgtgataccg taagcgattt cacctttggc
     3121 tgccagttca gccacagcgt ttgcagattt ttggatgccc gggatgcaag cctcggaaag
     3181 ctgcacccgg gtttctggat ggctcgcaac atgctggacc tgctcaattg tcagggactg
     3241 accgtccagg aaaatttgcg tcattggctg ctctccgcgc gaaattcgtt caggattcgg
     3301 taatgatgac tgtgtttagt gtaaccgccg gcgaattaat caggctcggg tcgcgcgggg
     3361 gaatagcaaa gagcacatcc agccccttaa caacctgacc gaagacagca tgcttaccgt
     3421 ccagccaggg tgtggggacg tgggtaatga agaactgcga gccgttggtg ttcgggccgg
     3481 cattggccat ggagagcacg ccgggcttgt cgtggcgcag tttcgggtca aattcgtcgc
     3541 ggaatttgta gccggggccg ccgcggccgg tgccggtcgg gtccccgcct tgggccatga
     3601 agtccgcgat aacgcggtgg aagatggtgc cgtcataaaa gccctgccgg gcgagaaaaa
     3661 cgaagttatt gacggtgcgg ggcacgttct tcgcgtacag gtcaatgaca atatcgcctt
     3721 tgctggtgcg cagggtagcc tggtaatttt tggtggggtc caactgcatt gtgggctctg
     3781 actgccattg taaggaactc atactgcctc cgaggtgttt tcgtctattg tagcatcaaa
     3841 cccgggggga gcccgcgctg catgcgtcgt cctgcgcgcg tttacagagg acagcacccg
     3901 aaactaagca gcactgcgat tatttctacg cgctgctggt tagcgaatgg aggtcttgga
     3961 gtagaaatcg gcggccgact gggaggaagg tcaagcgctt tcgttgttca ggatatccag
     4021 cagagaaagg atttcttcgt tggcgggaat atcggtcatg tcatgcccat agtggatgta
     4081 gcgcaccagt ccggatttat ccacgataat ctgcccgggc atacgcccca gcttgaagat
     4141 gttgacctgc tgccccatga gtttgagaac ggtgtgggta gggtcgggca aaccgataaa
     4201 gggcagcttt tctttggtga agtacgcagt gaaggcttcg gggctttcag ggccaatcac
     4261 cataatttgc gcgccggcag caaggaaatt ttcgtagtca cggcgcaact gcgccatatg
     4321 ccgttggcag aatggtcagg tgaatccgcg gtttagaatg aggatgacat gatggacgcc
     4381 tttgaaatcc gacagcctga ccggattgcc gtggacatcc acaagactga aatccggaga
     4441 agggagatta agggttacga gagtcatcga tgacctttca gaaaccgtgc tctacccttt
     4501 gacggcgccg ccgagcatgc cgcgaatgaa gaagcgctgg aagagaatat acacaatcac
     4561 ggtcggcagc gcgacaatca gggcgccggc tgccaacagg ggaatattag cggtatattt
     4621 cccctggaag aaggccagcc cgaccggcag cgtacgcaga gtttcatcct ggatcagcac
     4681 gagcgcgagc aggaattcat tccaggtcca catgaagatt agcacgacca gggtggttac
     4741 agatggtttg gccagcggcc agagcacccg ccagagcgtt tgccagcggc tgcagccgtc
     4801 catggcggcc gcgtccacca aatcacgcgg ggcgttttcg aaaaaggaag tcatccagag
     4861 cgtgccgaaa gagacgctga gcccgatttg ggggagtatc agcgcccaat aggagtcgcg
     4921 cagacctaaa agatccagga aatgataaag gggaatgatg actgcctcga aaggtaccat
     4981 gtatccgagc aggatcagcg gaaaaagcca gcgtttcccg ggcagcggca gcaaaccaaa
     5041 cgcgtaaccc gtcagcaccg acagaaaaac actggcggcg accacagcaa tggaaacgat
     5101 gacactgttc ttgaagaaga tgttgaaacg ccctgtgaac caggcttgtc tgaagttatc
     5161 caaattccag gtctgcggca ggctgaaggg accctgaata agctgggtgt cggtcttgaa
     5221 cgcgccgagg aacagcccgg aaatcggcag cagcaccatg aacgagaaga agaccagcag
     5281 caggtagcgg ggaaccagat ttttcacgcg gccgttcatg cgctttcctc ttcatttccg
     5341 gtttgcaggc ggcggatgag cagggaaacc gccagaatca tcagcatcag gagcgtcgcg
     5401 atagcggagg cgtacccaat ccggttttgc tggaaggcgg agcggtaaat caggaagccg
     5461 accaccagcg tggcgttgcc tggtccgccg cgcgttgtga cgtaaaccaa atcaaagacg
     5521 cgcagcgcgg cgatgatggt ggtgaccagc gcgacgccga tttccgcgcg cagaccggga
     5581 atggtgatgt gcacgaactg ctggaaagcg ttcgcgccgt ccaggctgga agcttcgtaa
     5641 tactcttccg atatatgctg catccccgcg agaaagagga tcatgcaaaa gccgtactgt
     5701 acccaggtgc cgatggaacc gaccgccggc agagccaagg caaagtcgcc cagccaggcg
     5761 cgtgttaggc cgctcaagcc cagcgcgttc agcaggatgt tgagcggccc aaattgcggg
     5821 ttgaaaatcc agcgccagat cacgcccacg acgaccatgc tgatgacttg ggggaggaag
     5881 agcaccgtgc gaaaaaaggt cattccgggc agcgctctgc ggctgaggac ggatgccagc
     5941 agcagaccga tgaagaccgg caaaacgcag taaaacagga tgaataccag gttgtgcaga
     6001 aacgcgctgg taaattgggc gtcggaaaac accatctggt agttttgcag cccgacccag
     6061 gttcgctgcg cgctgaagcc gttccactcg taaaaactgg tacggacggt ttcaaggatt
     6121 ggataaaaag tgaaagcgag atagatcaag agtccgggca gcaggtagag ccaccctttc
     6181 catttgtttt cgcctgggta agcctggttt gcacgcatgg ggtataaaag gcagccggcg
     6241 cttgttttga gcgccggctg tgataagcgt ccttatttgt tcaggtaagc ttcatagtcc
     6301 ttttggactg ctgaaacaaa gtcttcgggg gtgctgatgc tgccgagaag cttttgcagc
     6361 tccgccacga gcgtgtcgta gaaggtaggg gtggcccagt caatgtaatg accgaccgcg
     6421 ttgcctttat tgatggtgtc ccaggctttg agcgtatcgc caaagaggtt gtcggtctcg
     6481 atttccgcgc cttccggtaa ggccatcgcg gggagcattc cggcctttgc ccagagttcc
     6541 gcggcgcgcg ggctgatcat ccagttcagg tattccgcgg ccaggtctgc cttggtggtt
     6601 gtactgcgga tagcaaacgg aatgccaaca ccaccgatgg cgagggctgt ttttccttcg
     6661 aacggaggca tcaggaagaa gccgaactcc tggtccgtat cgttgatcag ttcccctgcc
     6721 agccaggagc ctgtgatggt catggcgcct tgaccagctt tgaagagggc gttgctgtcg
     6781 tcgtaaccaa taccagcgta gccttcggag aagtagccgg atttagccca ttcctgcaag
     6841 attcgcgcag ccttaatgtt ctcgtcagtt ttgaagctga cattattcac gccgtaagtc
     6901 aggttgttga tgtaatccag gctgaccagc agatgctgga tggcgctgaa ttcatgaatg
     6961 gcattccagc catccaggct gccgaaggaa attggagttt cgccgctttc cttgattttt
     7021 gcgagcaggg catcaaattc ctcaaaggtc tggggaactt cccagccgta tttcttgaac
     7081 attgttttgt tgtagtacac acccaccact tcagcggtcg ggctcacacc gtagagattg
     7141 ccctgcccga aagttttgcc gtcgggcgtg aagctattgc ggctggcaac cgaggtggaa
     7201 aagaccgagc tccagttgta tttggtcagg taggaatcca gcggaaggag taagccggct
     7261 tcgaccatag cgcccatatc gctgcgtccc tgattcacct gggcgacgtc cgggccgttg
     7321 gcttctgtca gcgcgagctt gagggtcagc ttgaggtcgt cgagcgtctt gacgacgcgt
     7381 tcaatcttca cgccggggtg cgccgcttca aattccgcgt tgagcgtgtc catcacctgg
     7441 ctctccacat cgcggtagaa ctgatcccat acgaccaggg tggtttcttc tgctggcgcc
     7501 tgggtctcgg ttggtttgct gccgccctgg caagcaccca ggatgaaggc gaagaccaga
     7561 agaatcgcaa cggaaaaacg tatccttttc attttgctcc ttttccaaaa gcgaaagttt
     7621 aaaaaattat accttataga accgcggctt taccggaata tgcatcgggt tggagaagtc
     7681 ctgaacaagg tacagcgacc agattccggt gtgtccagga gatagaaagg tcctgatgcg
     7741 tggatgaagc cgtcaggatg gatttttccc cggccatcaa ctttcttcat ttcgctaatg
     7801 tttcctgatt accgaaaact gactgttgcc cggcggcaag cggcgctatt ttgcctcggt
     7861 caaaccgctg ggaagggggg aaaaggggaa aggctgctat tgccttgtgt gtcaaggtat
     7921 tcgcattcca ttgtcataga acattatgga atagaaggat gatttcgtag ctcctgaacg
     7981 acattgggat atgtctattt ttctatttca gcccagattt ttggtaatgg cgggctgctg
     8041 cttgttgata taatgagttt gtggattgga ttttttcaga aagttttcgg atacagaaca
     8101 ttaagcacac ggacggcatg aaaatcaaca ggcgcttttc acacaacggg cttgccggta
     8161 atagcggaat gtgcttcaca tcaggttaat cccaggctgg gcttcggtcc agttttttgt
     8221 ttaccaacca accaacagga aaggacatac aggtggaaca gaagcaagaa gtctctatga
     8281 acgaagcatt ttcacacctc tcgtggcagc tgttgaaggt cactgagaat gccgccattg
     8341 ccgcggcgcg ctcggcgggc aaaggcgata agcacctggc agatgaggcg gcggtggacg
     8401 cgatgcgttc cgcgttggac gaaatgcgcg ggttttcgtc gcatatcaaa atcggggaag
     8461 gggaacgtga tgacgcaccg atgttgttca tcggtgagcg gctgggcaga ggaaacatcg
     8521 ccattgatat tgccgttgat ccgctcgaaa ataccaatgc caccgctgac ctgaagcccg
     8581 gcgcggtttg cgttctggca gcttcttcct ccggcgggct cattcaggct tcggatacct
     8641 accttgataa gctgtgtgtg gggcgcgaag ctgcgccttt ggttgatatc cgagcggaac
     8701 ccgaagaaaa tatccgccgg atcgccgcgg cgctgcaccg cgaaaacctg gctgaaatca
     8761 ccgtgaccat cctggaccgc ccgaggaacg aggaactcat ccggcgcgtc cgctccaccg
     8821 gcgcgcgcgt gaatctcatc atggatggcg acctgatgcc ctgcattttt acctgtttta
     8881 aaggcgcggg cgtgcatgcg gtgatgggca ttggcgctgc gccggaaggc gtcatatccg
     8941 cggcggcaat caagctgttg ggcgggggca tgcaggccgt ctttaagccc aaggacgacg
     9001 ctgaccgggc aaggctgacg cgcatgggca tccgattgga cgaagtgtat acagccgaaa
     9061 ctctggcgcc cggggaaaac ctgatctttt gtgccaccgc ggtaacgcca atactgacca
     9121 cgaccaaagc tgtgttggaa ggggtcagct tcttcccggg cggcgccaag acccacagcc
     9181 tggtggtatc gaacacgttc atgaattatg tccccaccac acatgtgctg gacagcggga
     9241 agttctcgca ggaaaaaagc gaattccgcc tgtggtagtt tggcgcagct gcccgggcgc
     9301 ctctccgcct ggttgacagt ctaagcccgg atggtaaaat tgcggtctat tacgcgaatg
     9361 aggttttcat gaagacagct ttaattactg gcatcactgg tcaggatggt tcttacctgg
     9421 ctgaattgtt actgagcgaa ggttatgaag tgcacggcgt tatccggcgc tccagcactt
     9481 tcaatacccg ccgaatcgac catctgtacc acgatccgca cggaaacggt gaaaaaccac
     9541 gcctgttcct gcattacggc gacatgggcg cgacggatac gctcctggat gtgatttaca
     9601 ctaccagacc ggacgaaatc taccacctgg cagcccaaag ccatgtgcgc gtcagctttg
     9661 acatgccaga atacaccggc gatatcaccg ggcttggcac tacccgcatc ctggaagcta
     9721 tccggaaaag caagataaac agccgcttct atcaggcctc ctccagcgaa cttttcggca
     9781 gcgccaggcc cccgcagaac gaaagcaccc ctttccagcc gcgcagcccc tacgccgccg
     9841 ccaagctgta tgcttattgg atgacggtca actaccgcga agggtacggc atgtactcgg
     9901 tgaacggcat cctcttcaac cacgaatccc cgcgccgcgg tgagactttt gtgacccgca
     9961 agatcacccg cgcggtggca tccatccgcg caggtttgca aaacaagctc tttttgggca
    10021 acctggacgc caagcgcgac tggggttacg cgccggaata cctggaaatg atgcatttgc
    10081 tcctgcagca ggagcaaccc gacgattatg ccattggcac cggcgaagcg cattccgtgc
    10141 gcgacttttt ggatgaggct tttggatacg cgggtatgga ttggcacaag tatgtggaaa
    10201 ttgatccgcg ctacttccgc ccgacggaag tggattacct gctgtgcgac ccgaccaaag
    10261 cgaaaaccaa gctgggctgg gaaccgaaaa tcaagttcca tgagctggta cggattatgg
    10321 tggacgcgga cctggaactg cttaatcaac ccaaacccgg cgagggcaag cgtattatcg
    10381 acgaaaaatt caacggctgg caccgctggg aacaccaggt cgtcagcatg gacaagtagt
    10441 ggttctgaca aaactgcgtt ataatggagt ttacggagta gtaatccgca gccgctgaca
    10501 gagaagggtt gggaaggctg aaacaacccg cagcacaggc acaggacgaa gtcgccggat
    10561 tgatgaccgc agaaatccga aaaggagaat agaccggtcc gggtacgccc gttagcgcgt
    10621 ttgagtgcat tgacgtatgt caatgaaatg aggtggtacc acggagactt tcgccctcaa
    10681 ccggcgaaag tttttatttt aatgttgagg aaaagaagat gaccgagctg ccaaagacct
    10741 atgactttaa ggaatacgaa gaaaagattt ataccgagtg ggagagcaag gggtattttc
    10801 gcccgacgaa tgacccgaac aagcccggac acgacccttc tatcaagccc tttgtcatcg
    10861 ttattcctcc gccgaacgtc accggcgggc tgcatctggg gcatccgctt tttgtcgcga
    10921 cggaagacct gatgatccgc taccaccgca tgaagggcga accaactttg tgggtgccgg
    10981 gcaccgacca cgccggaatt gccacgcagc tgcaggtcga aaagcagctg gctaaagaag
    11041 ggttgaccag aacgcagctc ggacgggaag ctttcgagaa acgcatttgg cagtggaaag
    11101 aacagtacgg cgggcaaatc acgcatcaga ttcgccggat tggcgccagc tgcgactggg
    11161 aacgggaacg ctttacgttg gacgatgggc tatcgcgcgc ggtgcgcgaa gcattcgtgc
    11221 gcctgtacga gaaaggcttg atttatcgcg gtcctcgcat gattaactgg gcgccaggcc
    11281 tcaagaccgc tgtttcggac ctggaagtgg agtactccga ggaaatgggc accctgtact
    11341 acttccgcta tcctctggcg gatggttccg gcgatttcat cccggtggcg accacgcgac
    11401 cggaaaccat cctgggcgat accgcggtgg cagtacaccc cgaagatccg cgttaccagc
    11461 acttcatcgg caggacggtg cgggtgccgg tgcttggacg ggaaatcccg gtggtcgcgg
    11521 acgaatacgt ggaacgcgag ttcggcaccg gcgcgctcaa aatcacgccc gggcacgacc
    11581 cgcatgacta cgaaattggg gagcggcacc acctggcgct gattaacatc atgaatcccg
    11641 acgcgacgct gaatgaaaac gccgggcctt atcagggcat ggaccgctat actgcccgca
    11701 aagcgctgtg ggaagaaatg cgcgcgcttg ggctggtcat caaggaagag ccttacatga
    11761 tgagcgtgcc caggtcccaa cgcggtggtg aaattgtgga gccgctggtc tcgacccagt
    11821 ggttcgtgcg catggggacg cttgcgcaaa acgccaaagc ggcagtggaa gcaggagggg
    11881 ttactttcgt gccggaacgc ttcaccaagg tcttccataa ctggatggat aacatccagg
    11941 actggtgcat aagccgccag ctgtggtggg ggcaccgcat tcctgtgtgg tactgtcaga
    12001 actgtgggga agtcattgtc gcgcgcgaaa cgccggaacg ctgcccgaaa tgcggcagcg
    12061 agcacctgga acaggacccg gatgttttgg acacctggtt ttcttccggt ttgtggccct
    12121 tctcaacgct gggatggccg gaaaagacgc cggattacga gtatttctat ccaacctcag
    12181 tgcttgaaac gggctatgac atccttttct tctgggtcgc gcgcatgatt atggacggcg
    12241 tggaattcac cggcaaggcg cctttccata cggtctatct gcacggcatc atccgcgacg
    12301 aaaagggtca aaagatgagc aagaccaaga acaatgccat cgacccgctg gaggtcatgg
    12361 actcgttggg gacggatgcc ttgcgcttta ctcttctggt cggcagcaca ccggggaacg
    12421 acatgaacat ctccgtgaaa aaagtggaat ccaaccgcaa ctttgccaat aaggtctgga
    12481 acatcggcag gtttgtcatc tccgcggtag accgcgccgg gcagagaccg gcggaggaac
    12541 cggtctggac cctgcccgac ctatggattt gggcgcggct caagcaggtg gtcaacagcg
    12601 tgaatggatt gttcgagaat taccagtttg gggaagcagg caaacaggtc tatgagttct
    12661 tttggggtga gttcgcggac tggtatctgg aagcttccaa gcggcagctt gcggaaggcg
    12721 gcagccgggc gtactttacc gccttcgggc tcgcgcgggt attggacgtg atgctgcgct
    12781 tgctgcatcc ctttaccccg ttcgtgaccg aagcgctgtg gggctacctg aaacaggctt
    12841 gcctggacgc cggcctgccc atccacagcc ggtctggctg ggaagaggcg ctcatcctgg
    12901 cgcgctggcc ggagcgcatg gaaatggaaa gctgggaaga cgaaatgatt aaagatttca
    12961 acaccaccca ggagcttatc cgcgccattc gcaacctgcg ctcggattac aaaatagagc
    13021 cggcgcgtaa actgcacgcg gtatttgttt cggataagca gcagattctg cttgagtctc
    13081 agaagctggt actgtgcgat ttggccggct tggacccgga attgacggaa atccgcacgg
    13141 aaaagccgga aagccaaagc gggatggccg ggctggtggt gaatgagatc gaagtgcttg
    13201 tgcagctggc ggatgccagg gatgacgcgg cggaggagga acgcttgcgg aaagagttgg
    13261 cagaagtgga gagccaaatc gcgcgcctgg aagctttgct ggcaagtccg tttgcccaaa
    13321 aggcgcctga aaagatcgtg caagccgaac gcgagaagtt ggagacttat cgggtcagcg
    13381 cggaaaagct gcgcatgcgg cttggttagg agagagggct gtcccaaaaa tcggacagcc
    13441 cttattgatt tcccactccg cgcccgtttt tatcggtttt tgcggatttg cgctatccaa
    13501 acgaggtcta caccaggcgt attctcccac tcaggtctgc caaaaaagga agacctgttc
    13561 tttttagaca gcctctgaag cacgaggttt agtttagcgg gctgagacgc gctccgactc
    13621 gcgctgcata atgcggcgca ggatggccag cgagctcgcg taggtggaat ccatgcccag
    13681 gtatgaaagc gcgccggcgc cgaggttttg acctttggtc aggggcgtat ccgaaacata
    13741 atgcaggatg ccgaggtcga acggcagttt gtgcaggttg acgatttcat tcaccgggtg
    13801 ccgctgcggg cggaaaagct cgtaaatcgc cgaaagatac gggccggctt ccatctcgat
    13861 atccgtatag ccttcgcggt aaatcacatc catgaccgtc gcgttctgta aaaaagtgcc
    13921 ccagacggaa accgctttct ggttatccag cacggtgcca taaacgagat acggtgatac
    13981 atctgaagcc tggaagttgt tatcaaagat ataggtgttc ttggaatgtt cgtcgtatac
    14041 cacatttggg agcacgacat cgccgcgcac gccgttcaga ctggcggcct tgcccatcac
    14101 gtaaatgccc agaatctgcg cggcgttttc cgcgattttc gtcagcaggt ggtaggcgcc
    14161 aaaaccaagc ggataatcaa tattcacaat cagcgcgtcg gatttcttca gaaagctcca
    14221 ttcggcgcag ccgggtaaag ggcagggagt gatgcggctg tcaacgcttt cgggattgag
    14281 tctggacagc tcaatcacct gcgcttcgat gtcaaatgag tgcacactcg ggaagcgata
    14341 gatgccgcgc tgcttctcga agctgagttg ttcctgccag gcatctttgc ctgccgatga
    14401 ggattggtat ttcttcatta cgtaatagaa gaagttttcc tgactggaag gcaccgcctt
    14461 gctctggatg tcttcccact cagccaatag ggcaggctgg tcggcactgt ggatgaaatc
    14521 gattaactga tcctgcctgc tgagcgcgaa gccggaaagc aggtttacca cgctgtgtgt
    14581 gttggaggac acaaaataaa tcggacgttt attgatatgc gggtactgct tttcgatttt
    14641 ttcccaccag gtctcagtgg ctttgcggta atcgttcagg gagccgctga ggagctgcag
    14701 ccggaagttg cacttcacct gcgcgaattg ccccatgacc tcgttgaaac gatctcccag
    14761 cacagtgtac agtctgccaa gctcctcctc gcttaactta agctggaggg cgagccgctt
    14821 gaaacgttca gggtccgaag gcgaggctgt ccgatacagt tcctccggtc catggctgag
    14881 caaggcgttc agtttgttcc attcgatttg cagcgcggtc agagtgggca ccacgtcatc
    14941 gatatccgag cgggaagcga tgtaacaggc catttgctcg cccccgtccc aaaagcagcg
    15001 ccgcctgcgc gcgcgggccg ccacttctgt ccagctttcc acattgccgt atccgtgttg
    15061 tgaaaaactg ctctcagttt gccctagaat gactttcttc acgtgaaaaa tgcactccgg
    15121 cagacgctgc aagctgtaaa tcagcgcgga agtgtctggg aaaggttcgc gagcatgcgg
    15181 atgaagagag gaattcatcc cggaatgtac ttcttcaagc gaacggattt gcacaacatt
    15241 ggtggaacgc agcagcgagt agattgtgcg taaatagaga tcgatttcct ctgaggaact
    15301 aatcggaact tgtctttcca taaagctttg catgtccttt gctgttaggt gcgcgtcaat
    15361 tgattttatc atgccggcct tccgcttgcc ggcatacctt ataatagggg cacgaaggag
    15421 ctgcgcatgc cgattgacac ggttattttt gattttggag gcgtaattct gcgctacctg
    15481 gacccgcaat ggctcaggga gaacatgcct tacggcgcgg aaattcagga agcgatttat
    15541 gaatatctgg tctcggatgt tctctgggag gtgatgcgcg gcagacttaa ggaaatggac
    15601 ttctggacca ggaaagcccg tgaaaaaggg atccccgtcc aagccgtaca gagctacctg
    15661 caagctcaaa acgcccgggc gcagctggac caacaggtgc tccatcatat ggctcgtttg
    15721 aaaggcaggt acaggctcgg gatactcagc aacgccagcg atacaacccg ggctttgttg
    15781 gaacgggaat accgcctgca tgaattggtc gacgacatcg tcatttccgc ggaggaaggg
    15841 ctggtgaagc ctgagcctga aatctacctg ctggctgcgc agcgcctggg tacgcagccg
    15901 gagcgctgcg tcttcgtgga cgacctgccg gagaatatcc agacggcgga aaagctgggt
    15961 atgcttgggg tactgttcaa agacacagcc aactccctgg cccgtttgga tgccctgctg
    16021 aagcaaggcg gtgcctgatg ctctacctgg tcgcgactcc gattggaaac cttggcgata
    16081 tgagctaccg cgccgtggaa accttgcagt tggtggattt actcgccagc gaagacaccc
    16141 gcaagaccag catcctgttg aagcattacg ggatctccaa accgcaggtc gccttccaca
    16201 ccatgaacga agccaaaatg acgccaaaac tgctggaacg tctgctggcg ggggaaaaca
    16261 tagcgctcgt caccaacgcg ggcacacccg gcatctctga cccgggttac tcgctcacgc
    16321 acgctgctgt ccaggcggga gttccggtta cttccatccc cggcgcttcc gcggtcatta
    16381 ctgcgcttac cctggcggat ctgccgctgc acagtttcac cttcaaaggt ttcccgccgc
    16441 acaaagaagg gccgcgcaag cgttttctgg agcaggaaaa ggactccccg cacactctgg
    16501 tattctacga gagcccctac cgcctgaacg ccttccttaa gaacgcttta gaggtctttg
    16561 gggaccgccg cgcggtcata gccaatgacc tgaccaagaa attcgagacg atttggcgcg
    16621 ggaatttgag cgaactccag gaaaccatac aaaatacagc catcaaaggc gagttttgta
    16681 tttgtattga ggggcgcggg gaagaaaagc ctaaaacgcc agaggggtaa ggtctgaaag
    16741 gtccgagtag gtatccaggt tttccgcttt tttcaggcgg ctggtaaccc aatacgtggc
    16801 gggggtcatg ataacttcga taccaacttt aaagaggtaa ttggtgaggg tgagcgtaag
    16861 gtacagctcc caggggaaaa cccgcgtgag cgatcctacc gcgataaaga ccgccgtgtc
    16921 caccagttcc cctacgatgg tggagctgat ggtccgcagc cacagccatt tgcctctggt
    16981 ggtatgtttc agtattgcca taatgactgc gttggaaaag ctgccgctca gatagccaag
    17041 gaagcttgcc agaacaatcc cgccggagct gacacccccc agaattttgt cataggaggt
    17101 ttgacccacc gtgttcaacc aggtgctctc ccccggtagt ctctggatga gcaggaacat
    17161 cagggcagtg aacgccaaac ccagaaagcc aatccaaatg acccgccggg aggttttgta
    17221 gccgtaaact tccgtcagaa tgtctccgaa aatgtaagcg accggaaaaa agagcgtgcc
    17281 ggcgtcgaag gccagaggga cgccgcccag cgagataccc cagtcaatca gcttggcaga
    17341 ggaggcgatg ttgctgagcg ccagcaccag cgtgaaaacg accgtgacga tatccaggta
    17401 tcggtaggtg cgcgcggggg ggcttggcgg ggtggtacgg ttcatcacgg ggaagtatac
    17461 cctttttaga aggtatactt attcccgcga tgcagagatt ttttgtggac cccgaagaga
    17521 tacagaacgg tctggtgcgc ttcccggagg gagcagcacg ccagatagaa aaagtgctgc
    17581 ggctggacct gcacaacggc gaggtgcaag tgctcgacaa cagcggaaga agctatcgcg
    17641 tgcgcttgcg cggcaggcat gccggcgctt tgtatggcga gattgtgcag gttctggaag
    17701 cggatccgcg ggtggaactg cggcttgaac tcggcttcag cctgacgaaa cgcgaaaaag
    17761 tggagtggat tctgcagaaa gggacggaat tgggggtcag cgctttcaga cctttcgtct
    17821 cagagcgcag cctggaccgc agcctgaggc tggatcccgc gaaacaggaa cggtggagag
    17881 cgatcctccg cgaggccgcg gagcaatccc agcgcacgcg cctgccttcg ctttttgccc
    17941 cgcgaaattt cggcgagggg ctaggcgatt ttccgcggga aagcgtgcgc ctgattgcct
    18001 gggaaggcag caagccggag cttcagttga aaccggattg gtttggcaaa agcaaagggc
    18061 agcccggcat gggggcgagc ctgctaatcg gtccggaggg cggtttcagc gctgaagagg
    18121 taggtctggc agaaagagag ggcttcaggc agttttcgct cgggcggaat gtactgcgaa
    18181 tggagacagc ctgcctggcg gcctgcgttt tgtgcgctca ctatcttgac aacgcccttt
    18241 aaggcgtgag gatcgcgcgc gaaggcagca ggttgcgcag tctgcccggg aggtttttac
    18301 cggcgcccag gtttctgcct tcagcgtccc gcagggcgat gatgctgctt ttccggcagc
    18361 cgctcacttc gaccccgcgg atatccgagc cctgcagcca ctggcgcgct agctcttcag
    18421 ggataaccca cacattgctg ccaaaacggt ccccgtagcg gctgatcatt tcaggggaca
    18481 ggtcaaaatt cgctccgctc cacttgccaa aggggaaacc aaacgcgtaa tagggcagat
    18541 ctgaaaaacg gcgcaggtac tgctccggaa gcagagaaag ctgctggtcc cggcggtaga
    18601 gcaggagttg gcgtttttcg gcctcagcgc ggaaatcaaa actgtacaga tccagcaggc
    18661 tggcaaacag ggccgtctgg tctttggcat ccaggggctc caaaccggtt ttgccgaagg
    18721 ggcgggaggg cggcgtctgg cggggcaagt cgagcggggc agtcttgagg agtttcgccg
    18781 cgaaaaagcc gttcgtgcca gccaggtgcg gccacaggcg caggctccgg gcggtctggt
    18841 gatcgaactc gcggccttca aagcgcgtaa gcccgggtgc gttcagccca gcgagcgggc
    18901 tgggtagcag gcgcgcgctg cccgggtgtt tttccaaaaa agcgctcaaa actgcttcat
    18961 cttcttcagg ggcgagcgtg caggtcgaat agacaatctc cccgccgatg cgcgccgcac
    19021 tgacggctga ttcgagcaag gcatactggc gcgcggccaa acgctcgcgt tctccgggcg
    19081 taatcgcgcg gaaggggtga ctggcggata ctctcaggcc ctccatgctg cagggcgcgt
    19141 ccaacaacac ccggtcaaat gtttcaggga accaggctcc caacctttca ccgggaaagt
    19201 tggtgacggc gctgttggcg gctccccagg taagcagcac tactcgcagg gcttgcaggc
    19261 ggctggcgct ggaatcattg gccagcagca ggctggcgtc gtcagcgctt tccaccagtt
    19321 gggtggattt cccaccgggc gaggcagcca tatccagcgt aagcccatcc ttcgggagcg
    19381 cggaaaacag cgccaccggc aaaatcgaag ctgcgtcctg gatgtagtag tagccgaagc
    19441 ggtgttcgat ggtctgcccg ggcgggtttt gcgcggagga aatttgccag gcgtcaggcg
    19501 cgaaagggat gggtttggct tgccagccgt agcgcccgct ccagcgggag aaggctgcct
    19561 gtgggttggc gctcttgagc aggtttacgc gcacagcaat gtcggcgggc tgactgatga
    19621 ctttgagaag ctgcgcgtaa tcctccgaag agaggcagtc tttgtagcgt tccacggcgc
    19681 tgagggtgag gtcattcacg aggctcgtca ctacatttcc ccttttcgga tgacgaagag
    19741 cagttcatcc gccacctgcc ggcgttcaac ttcccaaccg cggccgtcgc aagccttgtt
    19801 gatgagttgg gtatgataat cggcgagcga atgatgcccg ctcaggtcag cggcaggcag
    19861 gctgacgagc agcagcggcg cgcgcaaggc cgcgaagaaa gctccgttgc agccgggctg
    19921 gcgtttttca aaacgatggg cttccttgaa gaaaaaggcg caatccgcag cgagctcggg
    19981 cggttcagtc aggatatcct gatggattgc gcgcgcgttt ggaaagcgaa gcgcaaagaa
    20041 ttggctgagg aagtccaggc gcggtttgtg gatgtcgtac gcgtggaaat tcactgtttc
    20101 gggcaggttc agccacaaga gcatcagcgg gtccagcgcg caagccaggt ccagaaccgt
    20161 gtggggtgag ccaatgattt cgcgcaatac gccgcaaaac ccgtccagat ggtcgagccg
    20221 ctcgcgcgtg gaggcatgcc tggccattac gccggctgcc cactgctttt cctgcgcttc
    20281 gctcggattg gctccgaaaa acgcaagcat cttttcggtc tcagccgggt agtcgatcag
    20341 ctccagatac ggcgcgacga tgttatgcaa ttttttacga aagcctgcgg tgatttcagc
    20401 agcgctgccg cctttggcgg cctgctccgc aaggagctgg cgcagcatgt tctcgggcag
    20461 gtccaactca cgatattttc gtgaagtccg cacttcggca atgatttttg cattcaggct
    20521 ctcatccata ttgctcaccg ggagagcaga aagaccattt cgttggggaa gtcgaaggcg
    20581 cgtacgcgga aggccgtacc ttcaatcagc cgggcgaact gctgcgcgta agtcaggcgc
    20641 atgccctttt cacgtccgcc caggcttttg gcagggtagg aaaccaggat atgctcagcg
    20701 ttcaattctg caagcaggcg cggagctaag gacttatcca gttgctccag gcagggcagg
    20761 gttttgagca gaatcgccag ctgtactttt tgcttcggga cgcaagcgct caggtcgcaa
    20821 ggaaatgctt cagcatggat gccaactgtc gaaaagaatt ggttcaggaa tgtcagcatc
    20881 ggcaggacaa cgtcgcaggc ggaatacgta ccgttggcag ataaaggcat ccagggcagc
    20941 gccagcggat taagaccgca agccaggtcc aggacagatt gtatcggaga gatgtccgcc
    21001 agacaggtcg tgaagaattg ttcgatgtac ggcaggcgtt ccgcggtgga gttgtgcagg
    21061 cgcatcatcc gccggcaaaa aacctgcagc tcctcagcgt tttcaggcgg taagaccttt
    21121 aatctatccg tccacttcgc gtagttgata tctggggcaa ggaatgcgcc ggtgatttga
    21181 tgcaggcgtg tgcgtgcagc ttttagtgcg ctcttgcttt ccccacgctt ttccaactct
    21241 tcggcagcaa cgcgcgcaac caagctgggg gtaagctgcg cgtatttctc gctcgcacga
    21301 atcgcctgaa tcagcgtttc gagttcctca ggggagtagg ggctcatcgc gcagctgctc
    21361 catcaggcgg gtcatgaaac gcaggttgtg ttgggtggcc aggcggaagt aagcgctgtc
    21421 ctgcatgcgg tagagatggc tgaggtaggc caggctgtag tgctggcagg cagggcagtc
    21481 gcaaccgggg gaaacgggtt cctggctgcg gatatatttc tcgtcgttga tatagtggaa
    21541 agcaaaccaa tcctggcccc ggccgggaat tgcccgggag gagctgagcg tgtacagccg
    21601 gccgtggcgc gcgtcgcggg tgggcatggc gcagtcaaac atttcatagc ccaggcggta
    21661 gctggcagcc acgctgaccg gatgaccgat acccaacgcg tggaggggga actcccgcgg
    21721 gaccaccgag cgggtgtatt cgaggatgtc cgtgagcaga ttggactggt cgtccagcgg
    21781 ccagccgccg aaaccgtagc cgtcaaaccc gatttcgagc agcgcttcgg cgcaggtttt
    21841 gcgcagttcc gggtaaccgc cgccctgaat cacgccgaag atgagcggct tgtgctcgag
    21901 gtccagttta cgggaggaca actggctttg gaaagcggct ttggcgcgct tggcccactg
    21961 gatagtgcgg cttaccgaga gctgctgttc cgcgagtggg gcatccacgt gcgtgcagtc
    22021 atccaggcag atgagaatat ccgcgccgta gccgaactgg agctggatgc tcttttcggg
    22081 cgtcagaatc agcttgcgct gcgaggattc cggacggaag atgatgccgt tttcgcccag
    22141 gctgccgaat ttggcgttct ggcggatgag ggaataggcc tgaaagccgc cggagtcggt
    22201 catgatgagg ccgtcccagg ctgccaggtt gtgcagaccg cccagcgact gaatgacgga
    22261 tgagccgggc ttttgcatca gatggaaagc gttcatcatt acggcttgga cgcccacctc
    22321 gcgcaaatcc gcgctggtga ggctgcgcac ataaccgtag gtcgcgtccg gcaggaaagc
    22381 gggcagcggc aaagcgcggc cgcgaacttg aagggtgctc atggtccgcc gaactgcttt
    22441 ctctgcggcc ggtccagaat caggatgaat tccgccttgc ggttgttggc ttggcggata
    22501 atctcctcga ctgtcccgtt gtacagtttt tccccgggca gggtcaggtt gcaagccaga
    22561 aagacgcgct gttttttccc gaaagcctgc tgtacctcta aaaggagctt tccaagccgg
    22621 tagggcgtat ccatcaggat gataggttgg tccgctcggc tcaggcgggt gagcgcgctc
    22681 tggcgctgtt cggtttttgg cggcaggaaa cccgcgaatt gaaattgctc caggttaaac
    22741 gggcaaaccg acatggcggc aatcagggag gaggcgccag gaatcggcac gacctgaacc
    22801 ttcatttcgt caagtaaaat cagcagctgc cgccccgggt cggagaaaac cggggtgccg
    22861 caatccgata tgagcgccag cgactgcccc tgcatcaaca aaaccaggac ctcctggatt
    22921 aggctgcttt cgttgtgctc gttcagctca atcaacggct tttccaggcc aagctctttc
    22981 agcaggcgcg cgccgttctt ccgttcttcg cagacgaccg cgtccacgtt ggaaagcagt
    23041 tctacggcac ggcgggtgat atccgcggaa ttgccaatgg gggtggcgac cacatagagc
    23101 tttccggcag cgggtttagc ggtcatggat gtgaacctct ctcataaata ttaattatat
    23161 aacatgcgcc gggctgtggg cgcaaagcaa atatggcata atacttgaaa gttttctgga
    23221 tatgtattga gagctttcga taccaatccg ggaataaaca aagcgaggat attgcggttc
    23281 atcagaatgg ctaattccat acggctgaat cggtcctgtg tcctttgcac agcgaggttt
    23341 catgaaaaaa atcagtcggc tgaccaaagt ttccctgctc ctggcagtgc tcttttcact
    23401 ggataaactg ctgggggttg cgcgccaaat gctcgtctca cgccagttcg ggctttccgc
    23461 cgagctggat gtgttcaacg ccgcgaataa cctgccggat atgctcttca tgctcatctc
    23521 gggcggcgcg ttggcgattg ccttcatccc agtactcacg gaggtcctta ccaaggatgg
    23581 gcgggagaaa gcatgggcgc ttttttccaa tatccttaac cttgcctttc tgattaccgc
    23641 ggttttggcg ctgcttatcg cggtctttgc caggccgttg gtaagcggca atctgggcat
    23701 cgcgccgggt ttcagcggta cgcaaatcgc gctgaccacc aaattactgc ggctcaacct
    23761 gatcgcaacg ctggttttct cgctgagcgg cctggtgatg gcgggtttgc aggcgaatca
    23821 acactttcta ttgcccgcgc ttgcccctat tttctatgat ttcgggcaaa ttctgggggt
    23881 tctaattctt gcgccgagtg aatcctatca gataggtccg ctgaggctgc cagccctggg
    23941 actggggatt gaggggatgg tttatggggt catcctgggg gcggtcctgc atctgctcat
    24001 ccagataccc ggcctgaaaa agtatcattt tcgctggcag ccgcgcgccg acctgaaaga
    24061 ccccgagacg cggaaagtcc tgcgcctgat ggccccgcgc atgctgagta tgctttttat
    24121 ccagctcatt ttcctggcgc aggataattt cgcctcgcgc ctggcgaccg gttccgtcac
    24181 agcgctgaca tacggctggt ggattatgca ggtgccggag accttgattg gcacggcgat
    24241 agcgaccgcg atattgccga ccctggcaga attgttcagt cagcaggcat tcccgcagct
    24301 gaaaaccaag gttgagcgcg ccgggcaggt gatgctggcg ctgaccatcc cgatcgcggt
    24361 cattgccggc ctcgtcatcc aaccgctcat ccagctgctg ctggggcttg gacccgcgga
    24421 tacagcccgg gtggtgctgg tgaccagggt gttcctgctt ggcatcagcg ggcacgccct
    24481 ggtggaattg tttgtcaggt ctttttacgc catgcagaag ccgaaggttc cgctcatgct
    24541 ttcagctttc acgctgttgg tttacctcgg gtcgggcctg ctgctttccg cgcgttatga
    24601 agcagcagga attgcagccg cgaacacgct ggcatacacg ctgcaggcgc tggtgctctt
    24661 tattctgctg agtaaagttt tggcggagaa gttggagctg ctgcccacgc tggcgcgggc
    24721 cgtaatcgca gcgcttttgg gcggagcggc ggcgtggctg gtgatgcgct ttgcacccat
    24781 cccgggcggc gcgctggccg cttcaattgc cgccgggctg ctgggcttgc tgcccgcagc
    24841 gttggtactg cgcaaggaat tacgcgctct gaccagcctg taagcatgga gtataatttt
    24901 ctcattatgg acgaaaacaa cgaaaacaac acacaagaag cccctttggc tgctgaacag
    24961 gaaacgccaa tcgctgcggc tgaattcgat acagctgccg aaactgaagc agaaggcgcg
    25021 cctgtacgca aacgccgcgt gccttggatt tggctgggcg tgctctttct cattcttatg
    25081 ttagccttag gcacgttcct gggctaccgc aagggaatcg caaggcggct cgcccgcgaa
    25141 cagaccaccg tggtggaagt cgccgcggag cagtaccaga tggcgttcca ggacatggcc
    25201 gcgggcaact accagaatgc caaagcacgc atagagtatg tcatcaggat ttatccgcag
    25261 ttccctggcg cgcccgagct tctgcgcagc atcctgatga acatagagca gccgaagccc
    25321 acgcccacac tggcggccat cgcgcagctg acgcccatcc ccagcggaac gccggatacg
    25381 cgcagcgcgg aggaagcctt caatgccatt gccgccgctg ccgccgccaa aaattggcag
    25441 gccgtagtgg acaatgtgat tgctatccag cagatcaact acgactaccg gactgtggac
    25501 gtggctggtt attattacat cgccctgcgc aacctcggca tacagaaaat tcaagcgggc
    25561 gaacttgagc aagggctgtt tgacctctca tccgcggaac aaatcggacc gctggacgga
    25621 gaggcggaag gcgtgcgttc ctgggcggcg ctttacctca gcggcgccag ctattgggat
    25681 gtaaactggc agcgtgccat cgaaattttc cagcagatta aagaagctta tccctacatg
    25741 atggattcct cggggatgac cgcgatcgaa cgctaccggg tggcactgta ccgctacggg
    25801 gaccaaatcg ccgcagccgg agattactgc acggcttacc agtattattt gcgttcactg
    25861 gcagtcgcgc aagaccctaa cgtccagcag acggctgacc tgtacaagca gaactgcgaa
    25921 tcctcacgcc cgacagaagt aacgccgacc gaaccaaccc cgccggatga gacgcttgtt
    25981 ccgccgacgg aagtcacgcc ttctcctccc ccgacggaca atccgacccc gtagaatctg
    26041 agggtaaaaa atcagcccgg tgaacactta tcgccgggct gttgtgttaa ctcagggatt
    26101 actgttaagc ggcagatgtg ggaacgactt ccccatacag gtcgtgctgc atggcggctt
    26161 ttacgcgcac cgaaagcatc tccccgacct gtgctttgcc ttccgcaatc atcagaccgt
    26221 ctatctctgg cgcgtcgcgg taagtcctgc cgaccacgat gttgtgttcc gggtcggtgc
    26281 cttccaccaa catgctgaac accttgttct tcagttgctg gttcttgcgc atggagatgc
    26341 cggcctggag tgtcatcagt tcttcccggt gggattcttt cacagcttgg gggatgggat
    26401 ccccgagcgc ctcggcgggg gtgccttctt ccggtgaata tgtgaaaacc cccacatggt
    26461 caaattgcat ttcccgcagg aagttgagca aagcgctaaa gcgctgctcg gtttcattgg
    26521 ggaagccgac aagcaacgtt gtccggagga caagcgcagg gatacgcgcg cgcatttcag
    26581 taactgtccg gcgcacccag tctacatccg acgggcggcg cattgcgcgc agcacttcgg
    26641 gatcggcgtg ctgcagcggg atatcgaggt agggcagcag ctgacgggag ctggccatca
    26701 ggtcgatgag gcgcccggag ataaaacccg ggaaggtgta gagcagccgc acccacggca
    26761 ccctgggaat ggctggcagc agcgcttcaa gcaggtccgg aagcccatcc tgaatgccca
    26821 aatcctgggc gtaatctgtt acatcctgcg caatcaagtt aatttcctgg atgccggctt
    26881 cctgcagtgc gagcgcgtcg gacaggatat cttccttctg tcggctgacc agggaacctt
    26941 tgatcccggg gatggcgcaa aaagcacagg agcgccggca gccgtctgcg attttcaggt
    27001 agctgctgcg cccctggacg gtgtagcccg gcaggcctga aatgaagcgg atgcgttcgt
    27061 gctcggggat ggatacgcgg gcttgtggca cggttttggc gctcaaacgg gcaagcatag
    27121 gcaggatgtc cccaaggtcg cgggttccta ccataccgtc aattcccggc acctgctcca
    27181 acagcttctg ttggtagcgt tgggagaggc agccggcggc gattagtttt tggccgcgtt
    27241 tcttacggcg ggcaaatgct tccagctcgc ggatggattc ctcacgggcg tcgtggatga
    27301 aagcgcaggt attgacgatg aggaattccg cccttgaggc ttcaacgcag gtcagggaag
    27361 cctgattgat gagcaaatgc tccatgctgc tggagtccac caggttcttg gcgcagccca
    27421 tagtgctgat ggaaaatgtt ccgggttcga ttttcatggt tttggagtgg gtgagggggt
    27481 cagcaaatac gtaggcatgg acgagaggtc ggtcataccg gtggggtcaa agcgcagccg
    27541 gccgacctcc ccgacgcggt tgatgggttc cagggcggcg ccgttgaaca caaatgaaag
    27601 cgcggcaata ttcccggcct ccagttcgat gtactcgtca gcgctgtagg cggcagcgct
    27661 gccggccatc atcctgccgc gaaagacctc ttctccgtca gcgctcacgc gcacccaaac
    27721 agtctgacgc gcctgaacga ccacctggac cgaagccgtg gtgtccgttg gaagaatcag
    27781 cggggtcggg gaagggagca gcacaacctg ttcagtaatc tcgggggttg gggttggcat
    27841 gatcgccgcg ggggtttgag aacctatcag cacttccatc atttccggca gctgggtggc
    27901 atttacgcgg gtatccgggc gattcagcaa gtctgccgca ccccacaaca ggaaaataag
    27961 gatcccgcca accagaatcg tcccaaaaaa gaggtccagg gtaaaaaagc gccgcagggc
    28021 cagcaggaat ggcggaagcc tgcgggcagt ttttctcttt ttggattcag gcgcggcttt
    28081 ttccagccgg cgctcctgca gggcgtccgc gaagcggagc attaaaaggt ccgtgtccag
    28141 gttcaagaag cgtgcatagg tctgcagcag ccctttggct tcggataggc tggcgaagct
    28201 gtccagcagc ccttgttcaa gtgctacaag ctgctttctg gggatatggg tgtgttctac
    28261 cagtatttcc caggggatat caaggtactt gcggcgcgct tgcagttccc gcccgatgtc
    28321 atccagaatt tgctgggagc gcgtggtgga tatgggcaat acaggcggct gttcaacggg
    28381 ctgttttgct gccttgcccg ggcgcgggat gcggacatgc ctggctttgg ggctttttgc
    28441 cggggcagca ggttggggcg cggtttccgc aggttccgcc gggcttgcag gttgggttgg
    28501 ggtttgcgcg tcggcttggg aagcggctgg ctgtgggagg tcgggggccg aattttcagc
    28561 ctggacttcg agcggcacac ccagaaaatc cgcgtagagc tttaagaaac cgcgcgcctg
    28621 cgtgcgagaa ccgagctcgg catactcatc cgcctcgaga gcttccagca aagcgaggcg
    28681 aatgcgcgtc gcgtttgcaa cctgttcgag cgggatgcgc ctttcttcgc ggatggattt
    28741 cagctgagag ccggtcaggt cagacataag gtgattttaa caaaaccagc ctgttctgcc
    28801 aaatcggaaa caggctggct tacataaaag agatttgaac aaatttacgc ttcagcgtct
    28861 tctttggctt ccacagcggc tgcttcctca acagccgggg cagccgcttc gacagccttg
    28921 gcagggcgcg ccttgacggt ttcgccttcg cggtggacga taacctggag cagcgggacc
    28981 aaatcgatgg tcaggtgcac aggcacttcg tattcgccca gcgtgcggat cggttccagt
    29041 acaatctggt ggcggtcaag ctcgatgccc ttcagctctt tcacccggtc ggcaaccatc
    29101 tggctggaaa cagaaccgta gagtttgcca gtttcgctgg ctttcacggg gaattcaagt
    29161 ttgaggccgg agagaatttc cgctacgccc ttgagctcat tgttcagtac tgcgcgcttt
    29221 tcgcttgcct tatgaccgat tgattctgcc agtttgatgg aattctcagt ggccggcagc
    29281 gccagaccct gcggaatgag gtagtttctg ccgtatccat ccgcaacctt cttgatgtcc
    29341 cccgcacgac ctaacttata aacgtcctta atcaacaata ctttcatttc aagccctttc
    29401 taaagcttat cgttgttttc gcccggaatg aagggtacaa cacccgagcc gcaatattct
    29461 atcatatttc aaaactgtca aaccgaaagt gctgctggcg cccacccgcg ggatgaatag
    29521 attatactta cagaggttaa gcgactattg aaactgagga ttgatatgac tgatcaagag
    29581 agttttgaga aatggcggac ggaactttac gaaaaatccg tcagcaagta ccccgaacgc
    29641 aaagcctcgt ttaaaacatc gtccgaaatc ccgcttcatc ccatcgttcc gcccgaggat
    29701 attcatcctg aacgattttc cgagcttggc ttcccgggtc agtatccatt cacgcgcggc
    29761 gtccagccca caatgtaccg cggacgcttc tggacgatgc gccaatacgc gggttattcc
    29821 agcgcggaag aatccaacgc gcgctaccgc tttttgctgg agcaggggca aaccgggctt
    29881 tccgtcgcgt ttgacctgcc tacgcagata ggttacgacg cggacgatcc catcgccctg
    29941 ggcgaagtgg gcaaggtggg ggtgtccatt tcctcaatcg aggacatgga gaccttgttc
    30001 cgcgaaattc ccctggataa ggtttctacc agcatgacta tcaacgctcc ggctgccatc
    30061 ttgttggctg cctacatcgc cgttgcgaaa aaacagggtg tggacgcgag caaactgcgc
    30121 ggtaccatcc aaaacgacat cctcaaagaa tacatcgccc gcggaaccta catcttcccg
    30181 cccgcgccca gcatgcggct catcacggat acgatgcgct actgccggga agaagtcccc
    30241 aactggaata ccatcagtat ctcggggtat cacatccgcg aggcaggctc taccgcggcg
    30301 caggaggtcg gttttaccct ggccaacgca gtcgcgtaca tcgaagccgc gcaagcagcc
    30361 ggcatggatg tggatgaatt tgccccacag ctgtcgtttt tctttaacgc acacaacaat
    30421 tttctggaag aaatcgctaa gttccgggca gcacggcggc tctacgcccg aattatgaag
    30481 gagcgcttcc acgcacagaa ggctgaatcg cagaagctgc gctttcatac ccagactgcc
    30541 ggttcaacgc tcaccgccca gcagcccgaa aacaacgtgg tgcgcgtgac gctgcaggcc
    30601 ttggccgctg tgctaggcgg cacgcagtcc ttgcatacca acagcatgga cgaagcgctt
    30661 tggctgccga ccgaaaagtc tgtgcaggtg gcgctgcgca cgcagcaaat tatcgcctat
    30721 gaatccggtg tagcggacac gattgacccc ctggcagggt cttacctgat tgaatacctg
    30781 accgaccaga ttgaaacgca agcggctgaa tacatccgca agattgacga gctgggcggt
    30841 gcgctgaaag ccatcgagta cggtttcgtc tccaatgaga ttcaggatgc cgcgttctca
    30901 gcccagcgcc gcctggaaac cggcgaagat gtggtggtgg gggtgaacaa attccagcag
    30961 cacgaaacgc tcgaattgga atctttgcgc atcgacccgg tcatcgaagc tcaacagcgg
    31021 gaacgtctgg ctaagttacg ccggagccgg gaccctcagc gcacagctga actgctgagc
    31081 cagctggagc aagccgcgaa aggttcggaa aacctgatgc cgctctttat cgaatgcgtc
    31141 gaaagcaaat tgaccctggg cgaaatctgc aacctgctgc gcagcgtctg gggggaatac
    31201 cgcgccccaa atttcatctg aaggaagcca tgaaacccat caaaatcaac catgttgcta
    31261 ttgtcgtcaa cgatatcgac gcttcgctcg atttctgggt gaaagccttt ggcctgcaat
    31321 tggaccatat tgaggatgtg ccctcgcaag cttcgaaggt cgcttttttg cccttgggcg
    31381 agagcgaagt ggagctggtg cagcccacca gcacggacag cggcatggca gcttatcttg
    31441 aaaagcgcgg ggaaggcatg caccacctgt gcattgaggt cgaggacatt gaagcagcgc
    31501 tggcgcacct gaaaactttg gaagtccggc tgattaattc tgagccgcaa gtgctgcccg
    31561 ggaggaaaat ggcatttatc caccccaaag ctgccaatgg cgtgttgatt gagctgtatc
    31621 aactgacgga ctaagcctga ctcacctgtt ccagtctggc aacgctcagg gaaagccctg
    31681 accaacggcg atcaccggca gttcaaagga gagaaaatgc tgattaccaa tgggaaaatc
    31741 gtcatctggg gggataagcc tgaaatcgtt gaaggccagg cgctgcatct gcaggatggg
    31801 ttgatcgaaa gccttgcgga taccaacgcg ctcgtccaag cccatcctga agaggaaatc
    31861 ctggatgccg gcggacagct ggtgatggcc ggcaatatct gcgcgcacac gcacttttac
    31921 ggggcgtttt cgcgcgggat ggcagtcccg ggagagccgc ccgcggattt ttctgccatc
    31981 ctgagcagcc tgtggtggaa actggataaa gcgctggacg aagaaggcgt gcgctattcc
    32041 gctctggtat gcctggtgga cgcgattaaa tacggcacaa ccacgttgtt cgaccaccat
    32101 gcttccccaa actgtatcga cggctcgctg gatatcatcg cggatgcgct tcgccaggcg
    32161 ggattgcgcg cttcgctgtg ctatgaggtg accgaccgcg acggggaaga acgcgcgctg
    32221 gccggcatcc gggaaaatgc ccgtttcatc gataaagttg ccagaggggc ggagagcccg
    32281 ctgatcagag cgcattttgg cctgcacgcc agcctgacgg tctcagcgga aacgctggag
    32341 cgctgcctgg cagccaaccc gcaccaggcc ggctttcatg tgcatgccgc agagggattg
    32401 gtggaccagg aaa
//
LOCUS       DAOQAD010000010        31955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_1322_length_31955_cov_5.846834, whole genome shotgun sequence.
ACCESSION   DAOQAD010000010 DAOQAD010000000
VERSION     DAOQAD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 31955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31955
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1322_length_31955_cov_5.846834"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PKL60_01730"
     CDS             <1..502
                     /locus_tag="PKL60_01730"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNW94885.1"
                     /translation="GARWSVVPRRIAENYGAFTPWIALLFIPIAVFAKDILPWMNIEG
                     IKNPEYKTVTAETVLYKDGKEIARLNPAQHIYPDRQQYITIPDAHATLRGDVYTLLAG
                     YDVDIPYITLRLSDNPLVNFMWVGGVFMTIGGALAATLPARRGEREDETETMEETLDS
                     SGAADF"
     gene            474..1202
                     /gene="ccmA"
                     /locus_tag="PKL60_01735"
     CDS             474..1202
                     /gene="ccmA"
                     /locus_tag="PKL60_01735"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HNW94886.1"
                     /translation="MTLQARRISKAYGVQRVLDKLDLSIEAGEVVCLLGPNGAGKTTL
                     IRLLSGLQKADSGEVLWGTQDGNLSVLTLRRSIGLILHQPFLYEHLTGFENLRFYSRL
                     YETGLSDADLRKMLGRVGLGSARPIPVRGYSRGMKQRLTIARALLCDPQILLFDEPYT
                     GLDQQGCGMLNQLLLEEGARDKLVLLTTHELSSIRQVATRFDLLHRGKIAASIPNDGM
                     SLDALQDQYNAVIAAGKSLQKEQA"
     gene            1199..1873
                     /locus_tag="PKL60_01740"
     CDS             1199..1873
                     /locus_tag="PKL60_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNW94887.1"
                     /translation="MKAWLHSVRVLVWKDLLQEWRSRDILTSMLAFAVLSLFIFNYAL
                     ELSPINRAEIAPGVIWVVMVFAGTLGLNRSFTAEQDQGCFEGLMMAVPDLSAIYFAKL
                     LTNLLMMVFLAVLVIPIYSLLYNQSLFNQAFFGVLILGAWGYSAAGTLIASMTVQTRM
                     RDLLLPVLLFPLLLPVNMAVVKSCGVILGSVAGQDIHGWLNLLLVYDIVVTILSIMVF
                     EYVIQE"
     gene            1857..2561
                     /gene="ccsA"
                     /locus_tag="PKL60_01745"
     CDS             1857..2561
                     /gene="ccsA"
                     /locus_tag="PKL60_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNW94888.1"
                     /translation="MSSRNKLLPLLSVLASLLLLFAFLRVLFFTPVEQTMGLVQKVFY
                     FHMSAAWVGMLAFLAAAVFGLLYLSRRDMKWDTLTAASVEVGLLFTIICILSGMIWAR
                     PIWNTWWTWDPRLTTVTIMAFTYIAFILLRRGVDNPTRRARFSAVYAVIGFISVPLTF
                     FSARLLRTIHPVVFGSGEADMNLNPQMLGTLFISLGAFTVLYAALLLHRSRLMAEQEA
                     LNQQLAADDSHYDWGE"
     gene            2830..3975
                     /locus_tag="PKL60_01750"
     CDS             2830..3975
                     /locus_tag="PKL60_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNW94889.1"
                     /translation="MKKSTLFVLLAVVALALVAWVGCTTKEAVEEPAEETTSGINVVA
                     AKWAGSAHAEASSEAFNHWNEEDPQEVPPTCAKCHSSTGFQDYIGADGSEAGKVDAAG
                     KIIDPINCATCHNDAADKLDAATLPNGTQLADMGKNAICSTCHSGMGSSADVAKKVAG
                     LAEDDVIADQGIMGVHYLAAASTQAGADGGMGFQYEGKTYAGTFKHAEPVNSCTECHD
                     PHSLHTQSVPGSDANLCSTCHSDVKSYQDYKKISMSKVDYDGDGTAESTYDEIEGMRA
                     VLIKALAGYSTAKSGATFGFNPAAYPYAFIDTNADGTLSAEESAFPNQFKAFTPRMLK
                     AAYNLMFVTKDPAAYIHNADYALQLMYDSIESLSEFSGVSTQGLVRP"
     gene            4017..5309
                     /locus_tag="PKL60_01755"
     CDS             4017..5309
                     /locus_tag="PKL60_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94890.1"
                     /translation="MAKKTKLIIFVALCIALTLTPLVIMLVRANWALPIRDVSVALAF
                     FGMAVVGMQFLPISRIPWLSEVVELDKMYKIHHTVSAFAVLLVALHPLILLVTGSVYF
                     HGFLMIAGWIGLGGLVLIAITSIYRKQLKISYTTWLLIHDILTLLILVFGLLHMFQRN
                     YYLRDTGMTIAWIILMCIWGGLALYIRVIRPLINARHPYAVSKVVEEGKDTYSVYLKA
                     EGFKRPAFNAGQVAWISKGPSPFVISRNPFSYSGSTEWDELRFSIKKAGDFTNTIPDL
                     KQGDRMFVDGPYGTFNLKNPRMQKGLVLLGGGIGIAPVMSMVNTLADTGDKRPVYVFY
                     GDLCEDTVLFANEFEALKVRMNLTVIQVLEKPLDVEKCKHKGYITKDLLLSTLPENYK
                     ELYYFMCGPMPMMNAMNKHLKAMGVDPAQIATEKYEMA"
     gene            5475..5600
                     /locus_tag="PKL60_01760"
     CDS             5475..5600
                     /locus_tag="PKL60_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94891.1"
                     /translation="MPNNPDHDHIPSKTLDRKLNWILFLQIVLIALILALYVLGK"
     gene            5807..6943
                     /locus_tag="PKL60_01765"
     CDS             5807..6943
                     /locus_tag="PKL60_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNW94892.1"
                     /translation="MTRAFGVDISKYQTSQDGTKRQDFNALRAHTEEVVFIAARAGVC
                     WGYKDPMFDYYWSEMARIQVCRMAYLVVYFGESAISQMDALFKIVEGQANWTHDRLVL
                     DLEVAGINTRERITSTTQKCLDICKARTGIYPICYSRASWVNAYLKIDQLPRLDWWLA
                     TYKKPFPYPAYTPEHPGPPDLPRGVATYLIHQTGDKNKAIGSASRYMDFDRWNGSKTD
                     VFRYFNNPDNGNPNPPPPDPGAVLFRARCVVSALYKREGPGTRYSVVGSLNLGEEVNV
                     YEVKEDWFRIDPQASVWCSGNARYMRMLDPGQPDPVKQPIFRVKVIVTALYKRRGPSA
                     SYAITGYLQKGEEVSVYEVTNNWYRIDAENQVWCSGAPQYTQKI"
     gene            complement(7003..7173)
                     /locus_tag="PKL60_01770"
     CDS             complement(7003..7173)
                     /locus_tag="PKL60_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94893.1"
                     /translation="MKLSAPKFVTFLVALVLALAGLLAQLGVIAVLAQYAFWLVLAGF
                     VLLALAVLLNDL"
     gene            7424..8161
                     /locus_tag="PKL60_01775"
     CDS             7424..8161
                     /locus_tag="PKL60_01775"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNW94894.1"
                     /translation="MSQDSTPPSLPGISISRNGPYIVEGAVPLVRKTQIVSEYGEPLS
                     WQLLSRYELPEARYRLCRCGNSKQMPFCDNTHLLKPFQGKETASTDGSEDDSIVLPGG
                     KQIIVHLYRDLCMSSGFCNLRNTGIVRLTAESEDTLKRSLAMAMIERCPSGALTYQLD
                     PDEPEVEPDLPQEIAETFEITSHGPIRGPLWVTGYLKIKRSDGVPFAARNRVTLCCCG
                     NSRNKPLCDGTHRRDAEWEAEWHRKRK"
     gene            complement(8172..11018)
                     /locus_tag="PKL60_01780"
     CDS             complement(8172..11018)
                     /locus_tag="PKL60_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNW94895.1"
                     /translation="MPAKQGYNSGMATLDLKVNGSSYSLPEIPGETLASLLRGRLHLT
                     GTKVGCGEGRCGICTVLVDDKPVKSCLYPARKAAGKSLLTIEGLRALSPTPGSPHPLQ
                     EAFTSYGAIQCGFCTPAQILNAYAYLLQHPDPDYEELKAALKDVLCRCGTYEAILQAV
                     LAAAAVMRGRGPLPAPKVSLVPAETSVIGTLHPRPDAAAKADGSAVFTDDLAFSGMLF
                     ARVLRANVPHAILHSLDISRALALPGVQAVLTAKDLPAARLHGLFTSDWPILVGIGER
                     IRTVGDALALVAADTQAAADAAIALIQVGLEPLPVLSTPQQALAPQAELLHEKGNLLK
                     HIEVRKGDPEQGFRDAEIILEHSFFTPFTDHLFMEPECSLAVPLPDGRMEVYCGSQIP
                     YSDRSQVAAALGVPEEQVRIRGQLTGGGFGGKEDIAGQIHAALLARATGRPVKLLFSR
                     RESLLVHPKRHATQIRVKLGARKNGLLVAAETELFGDTGAYASLGEKVMTRATTHSTG
                     PYVIPHVKSDCYAVYTNNPPAGAFRGFGVLQSAFAVESMMDILAHSLGMDPLELRKIN
                     ALHAGSLTNTNQLLEGSVGLPDCLRAVERALREAAGAEPFKPTRLTRGGKQYLRCWGT
                     AAAYKNTGLGGGADDAGAADLELCLDGRMQVRSAAAEIGQGLVTTLQLIAAEVFSRDP
                     RDVDVLVMDTDLTPDSGPTTASRQTFVTGNAALLAAKELKQHILANLSARWDTEPEQI
                     RFSSEGARTSPQDEPDAAHSMRWPEIAALMDASPAGRSIRRVYHAPETSPLGGPAPIH
                     FAYSFSAQAVEIEISLEDGGVQVLQVISANDAGRVLNPLGFQGQVEGGIVMGVGHALM
                     EEFRVNEGVVETDRMARYPIPRMPDSPQVKSILVEHPTAEGPFGAKGVGEIVSIPTPP
                     AVANALFNAIGLRVFRLPIRAEEVLAYLREHPQA"
     gene            11195..11668
                     /gene="bfr"
                     /locus_tag="PKL60_01785"
     CDS             11195..11668
                     /gene="bfr"
                     /locus_tag="PKL60_01785"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNW94896.1"
                     /translation="MKGNTKVLEVLNDLLSDELTAINQYMVHGEMAEDWGYPVLHEAI
                     EKRAITEMRHAEKHIARILFLEGMPIVSNLKAIHIGADVPKMFANDHAAEQEAIDKYN
                     AAIKVAVEAGDNGTKEMLDAILVDEEAHIDYIETEQTKMEQMGLTFYLSTLTKED"
     gene            complement(11785..11919)
                     /locus_tag="PKL60_01790"
     CDS             complement(11785..11919)
                     /locus_tag="PKL60_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94897.1"
                     /translation="MPISPGSQPEKWRVNLSRVRRSNGVEGTLLISSLPSSGDPFIRT
                     "
     gene            complement(12360..12491)
                     /locus_tag="PKL60_01795"
     CDS             complement(12360..12491)
                     /locus_tag="PKL60_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94898.1"
                     /translation="MQPMRCDRALEGKTIVCIGLFRSIPGGTPFRTDITSEGENNVE"
     gene            13105..14331
                     /locus_tag="PKL60_01800"
     CDS             13105..14331
                     /locus_tag="PKL60_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94899.1"
                     /translation="MMRSRFLLLVLLTAACLLVLQACGPTPTPRPLPTEEPTLPPSLP
                     TEEIPPTETATLEPTPIPTSTEASAPPNGQIVFRYEANLWRYLVDAGTLEQLTFDGIP
                     GDYGSAYDHTEISPDGAVLAYTKGESSFMTDFTSGGSADMTTYGKFIKWNGVGRQFFT
                     ALGDMTCPPIENLEDQNLLNFDVMRYDQSNLSSATNLGNIGGGLKFVSTISNDGQWLV
                     ANSCGCYSECGNENLWHLPTLSQIDSPLSFPIGGLDFSADSTRLVLGQWQMYGYQESA
                     LYVAGSDLSSPNPIYSRADTAPVRQEWSPDGSMIAFTLVEIDPVEMSATERMVILISP
                     NGAEQLLVESGGAEFLAWSPDSSRILYTREVGGVRTVYFYDRVSETRTLLPFSIDAYS
                     DGAVDWGRLSAADLGL"
     gene            14432..15286
                     /locus_tag="PKL60_01805"
     CDS             14432..15286
                     /locus_tag="PKL60_01805"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNW94900.1"
                     /translation="MDTPEFEIYLPGAEQTASSTMPVQALAAVTHLGIGAHPDDLEIM
                     AIDGILKGFSGGDNVFAGVVLTDGRSAPRAGKFAALSDPELAALRKEEQKEAARIGRY
                     GALVFMNFVSRDLRQGGGVVESLGQVLQAARPRVVYTHSLLDAHPTHAAAAWRVIQAL
                     RGLPAEMLPEQVFGCEVWRGLEWLPKRFRVAFDCSEHPRLQTQLLAVFKTQVESGKRY
                     DRAIPGRRAANATMNEPDAVDQTRALAFAMDLNPLVRDKALSARAYLREILRDFEREA
                     LSALPDDA"
     gene            complement(15396..16229)
                     /locus_tag="PKL60_01810"
     CDS             complement(15396..16229)
                     /locus_tag="PKL60_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNW94901.1"
                     /translation="MDHIDPRARWIFSFAVLVAATSTWNSAILAILFLIAIIWYSLGR
                     TTWKETHRGWTLVLIMLFTMIVVNTIITGGGAGGVVPEGGKLVWPEGLRLFGKLLKPG
                     LTVERIWFAICQLMRIGSISMFFILIPYTMDSRSYGATFSKLGLPDKLAYTIELAIRF
                     IPSLARSFSITLDAQRARAFELDDVKGGIIQKIGRIAPLIIPVTMNAILSSEDIANAM
                     DLRGFGQRKRTWLFELKYHWWDYAVIGFGLLLTATCLILAHVYKLGAFTVPAFFYQLF
                     Q"
     gene            complement(16322..16876)
                     /locus_tag="PKL60_01815"
     CDS             complement(16322..16876)
                     /locus_tag="PKL60_01815"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNW94902.1"
                     /translation="MQEKKSLWKFGTKQVVYAAIGAALYAVLAYVTNIIQLPGAGNVS
                     IRPAVVIPMFFGIAFGPIVGFIAGFVGNILADLLSGYGFWFWWDLGNGLLGLVPGLIY
                     PLIQDYKAGKSILLAELFVVLGAAAGMGVASLSELWVSGADMKTVIFANFIPAFIPDV
                     LNGLVLVPLLMIAYAAITQRSGRS"
     gene            complement(17224..18846)
                     /locus_tag="PKL60_01820"
     CDS             complement(17224..18846)
                     /locus_tag="PKL60_01820"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW94903.1"
                     /translation="MTEPEVLPLIIRDLSFRYNTRSIPAIKNINLELRPGEVLLLAGS
                     SGCGKTTLLRCINGLIPEAYHGEMTGELLLYGESLRGKNLADISQNVGTLLQDPERQI
                     LGTYVLNEVCFGLECLGLERQEMIRRGEDALRRLNILHLRDKETFGTSGGEKQKVALA
                     GVLAMQPKILLLDEPLASLDPASAREALLAFRQLADDGLAVMIVEHRVEDVLAIHPER
                     VVYMEDGEITYQGGPAGLSQVVDYHKIKLPAADILARAKDAPETEFVPAVPPLDYSHP
                     LIQFKEVGFRYHEDLPEVLHGVNLNIHTGEVIAILGHNGSGKTTLVKHALGLLKPTRG
                     SVLLEGIESKSTTVAKAARTVGYVFQSPTQMLFAATVRDELAFGPKNLGFSKEIIQKN
                     VDWAVQSVHLEEELGTPPLALSFGQQKRVSIASVLSMRSRILMMDEPTAGQDYWNYQS
                     FMDTILQMPGFDSILFITHDLDLALTYANRILVLFDGRIVADGTPQEVLKDSEQLLQW
                     RLLPTSLLELNLRMFPRTGKFQRAEALARYIG"
     gene            complement(18839..19630)
                     /locus_tag="PKL60_01825"
     CDS             complement(18839..19630)
                     /locus_tag="PKL60_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNW94904.1"
                     /translation="MSIISLTTDFGNLNGFVGTLKGVIWSIAPGAQIADITHDIPPQD
                     VRQGAIALWRAAPFFPPGSVHIAVVDPGVGTQRRPIGARFGNQFFIAPDNGLLTPMLE
                     EAEAAGESVQVVCLDQPRFWLPNVSRTFHGRDVFAPCGAHLANGVALSELGTPVTDIM
                     RKPLPAAEQTENGFRAHILLADVFGNLTTDLPASRVRNHAHVTFSIRGRTIEGLALSY
                     GHKQPGDLVALEDSEGYIEIAVVNGSAKNALSTQIDDIVEVSLND"
     gene            19866..20360
                     /locus_tag="PKL60_01830"
     CDS             19866..20360
                     /locus_tag="PKL60_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNW94905.1"
                     /translation="MAPERMIFYESLKAIFLHIDNHEKAFLSRYNLSIPRFYALLHIY
                     NHPGINYIELSDLMLCTKSNTTRVVQGMQKDGLVLRKAHPADRRSFQLSLSKSGSLLF
                     QEVYPEYLALIDRLMSLFSDEELERYAAASNHIENTLAPENLANHRGALRVENPQPVS
                     PLLN"
     gene            20427..21965
                     /locus_tag="PKL60_01835"
     CDS             20427..21965
                     /locus_tag="PKL60_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNW94906.1"
                     /translation="MKKSVMLVAILLIASLVLGACAKATEAPVVTEAPTEAPVVTEAP
                     TEAPTEASAGTLRIWADEQRAPVLAELGKQFKAEYNVDVVVENISGIRDQFTVAAPAG
                     EGPDIIVIAHDQMPALVASGLAAEIDLGAKKADFLESAIKAFTYAGKLYGMPYAQENL
                     GFFYNTELVPTPPTTWQEVHDISQQLIADGKVEYGIVLGGTSYDAYPWMTSQGGYIFG
                     LDANGDYNPADIGVGGEGMIKFGQMALDWVNDGTLSNNTDNTTAKSMFKDGNVAFFMN
                     GPWEIKNLREAGTPFAITKFPDGGTPFLGVQGFVVNAFSENILLAQSFLTEFVATEEA
                     MLKLYEADPRAPAYKPALEKMDDPDLLAIAEAGEGALPMPAIPEMGAVWSDWGNALTF
                     IFNGEKTPEVAYTDAANNIKIAIASKGMVNVPGSYQTKVGCASDWMPYCAQTALVKGD
                     DGKYTGTFLIPAGDYECKVALDGGWAVSYGDGDANYKFTVAADGNVTFIFDPETTTLE
                     IIVP"
     gene            22115..23719
                     /gene="malF"
                     /locus_tag="PKL60_01840"
     CDS             22115..23719
                     /gene="malF"
                     /locus_tag="PKL60_01840"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008232.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HNW94907.1"
                     /translation="MGETTITKAKFAKRTISGVSSIITTAIVLIVDALLGYFTYRLFS
                     LGYIPLGIVFVLIIAIISFAFLVPKAHMFRWMAVGLAAWLLFSIFPILYTIYNAFTNY
                     GDAHLITQTQAIAQIKAQTYLPETGKAYQWVAYRSPENNYLLWLKDAEGNTSIVRMVD
                     AKADDHTLEVVPGQNGIGELDEEGAPLTIEGYTRLNRITAATDKNLTNIKFGNPEKTI
                     QVRSPSEAAELLPLFVYDPDTNTFTNQQTGVVYTDIEGTYTARNGDKLIPGYSAVVGT
                     KNFVQFVTAPGLRGPLATIVVWNFLFAFMSLFLTFSVGLLISIIYGDPNFKGKKLLRS
                     LLLIPYTIPSLITILIWRGMLNPDLGIVNRILESVFGVSPDWFNVPWLARTAILIVNT
                     WLGYPYWMLVTSGALQSIPSDIYEAAEVDGATGWQRFWNITLPLLLVSVGPLMIASFI
                     FNFNNFNLIYAFINGGPPIPTATTQAGYTDIIISYVYNLAFASGRGVQYGYASAISLV
                     LFVLIAIMSLAQFRFTNMWEEVGENV"
     gene            23712..24587
                     /locus_tag="PKL60_01845"
     CDS             23712..24587
                     /locus_tag="PKL60_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNW94908.1"
                     /translation="MSNKPQSIKRSKRAGLILRWLLAIVLVTFSIFPVLWIVSAAVNP
                     VTDMANQQLIPANADLSNFRELTTNPVFPFFTWLKNSLKVSILSTLLTLSLTTMAAFA
                     FSRFRFRGRQGLLKAILLIQSFPNLLAIVALFSIVKQIGDVIPALGLNTHAGLILVYS
                     GGAMGMNIWLMKGYMDTIPRDIDESAHVDGATDWQVFSKLILPLLRPILTVIGILSFI
                     GTYGEFIIARTLLTKREMQTAMVGLQIFTAGQYTQNWGVFAAGALIAAVPIMAIYLIL
                     QDQIVGGLTQGAVKG"
     gene            25005..26426
                     /locus_tag="PKL60_01850"
     CDS             25005..26426
                     /locus_tag="PKL60_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94909.1"
                     /translation="MKIRLFVIAALILTLMTSGVSVSADSAVDAEIYNTWSVATIEEN
                     ADEAVNISTIFVGKREIPMMMYVQENHNAIYWVWEATEDSPGNCGPDNAWHCDSTATT
                     FPFMNISNLATETYPDTFSVGYSYAAGGNLWGNTREYTHSMEFISSDSEVLLDFGEFG
                     ETVALAGPPSIAKIGGHFRGAVTLKVGVDFPVYKLVYIYFKGGSNDSCRSGVSSGYDC
                     IVIEETAAGGLMGAPALTSKDGTSRLLYYKDNAIRYAYPWETAGLKPSNCGETGFTWR
                     CIDLDAPASPTTVGAKVDMAVGRDASDLAAAYTLKGSPGSDYDSLMHASYVGSGGNCG
                     TDKNFLGENIFTWDCGDVDNWIDSISDTRFHIAFDNRNFPVISYNNKLIDDASSRQRL
                     YVAYWPGRVGLPGNGWDQYLVDGNDQSTTGYYSNIAFSNNGRVMIGYLQLFFQWNMII
                     DDTPNIKIATQWFSHTCLPFVVK"
     gene            complement(26675..28006)
                     /locus_tag="PKL60_01855"
     CDS             complement(26675..28006)
                     /locus_tag="PKL60_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW94910.1"
                     /translation="MRFSDLVNLIVANLARRKGRVLLTAVGVVIGTAAVVTLVSLGAG
                     LQKNATESLWGISDLSNITVYPGYPMEIKGGGGGGGGGGNGSDQQELVLITPSVIEQI
                     KAIPGVKRVIIQDYIAVGSEITFDKLTSWGNFQGVDVADLADMNLEASQGTTELGRGK
                     LVIGAYINRNFYDPNPRPNADPIEPPDLMGKVLKVTLIKWNQDGTETRRSYNMEVVGV
                     LKESKGESDYNMYMTLDELTRWNEWGIGRKINREKEGYNQVLVKAESPKEVVAISEQI
                     INMGLQAYTPQAMVQGINSFFTIMQIIFGGVGAIALLVAAIGIANTMTMSILERTREI
                     GIMKAIGATNNNILTIFLGEAAGIGFVGGVGGAALGWIISAVINVFASSYLASQAASN
                     GYMSSTPIATATPFWLPIFSIVFATLVGLLSGLYPALNAATLVPVDALKYE"
     gene            complement(28063..29244)
                     /locus_tag="PKL60_01860"
     CDS             complement(28063..29244)
                     /locus_tag="PKL60_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94911.1"
                     /translation="MTFSSEDFVPLDGGVFTLESIEINDAPGTTLRFRVSDYSSWKYS
                     GVINATATYTDDAGTSYSTSFVFYVAIAQSAAASPTKTSTPGAAAKPQLVISGYSTDV
                     DPLQPGSAFKLKLKIANAGVADASNVSLVYGGGVSLAPNEQGTPQPGISGSTGDLTNF
                     APVGSSNVVLLGELPVGASVETEQQFVVNVSTVPGAYPLKVSLVYTAPGGTRAVDDQL
                     ITLLVYALPQLEVSFYRDPGMFYNGMMTALPIQITNLSRKTTVLGNMTVSSENADLQN
                     NTSLVGTLDPGGYFTWDVMYTPFGEGPTKIHVSINYTDDFNQLRVYETALEIEVQPPM
                     EIPQEPLPGEGEMPVEPEQPVGFWAKIWNVIKGFLGIGTGSSTEQPVEVPMEEGSKNV
                     P"
     gene            complement(29460..30191)
                     /locus_tag="PKL60_01865"
     CDS             complement(29460..30191)
                     /locus_tag="PKL60_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW94912.1"
                     /translation="MSDDFVSTADLKKHYRMGETDVRALDGISLQIRQGSFAVIMGPS
                     GSGKSTLLNLIGGLDWPTSGSIRVDGEEIAALDENALAAYRREKLGFVFQSFNLVSSM
                     TAEENVGFPLRFSGIKSSVRKERAREKLEEVGLADRFNHKPTELSGGQQQRVAIARAL
                     INNPLLILADEPTGNLDTSSGTAVMHALVDLQKGGKTILVATHDPRMLSVATDKIFLL
                     DGKQVSEAQYHESIEKMTWEGSESR"
     gene            complement(30203..30988)
                     /locus_tag="PKL60_01870"
     CDS             complement(30203..30988)
                     /locus_tag="PKL60_01870"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNW94913.1"
                     /translation="MAETTIEKRKTPAPEMHWRWLPGMLIKPRKTVQAVLAEDKAVWL
                     TPLLVLSVLIIAAVLVSAPIQRQIIQSGSNIPENFQYWTSDEQNAFLQAQASQTSPMF
                     LYVFPVLSRVSGYWLIWLLTSSLLHLAITLRGSRSSRVRSSNLVAWAMTPFILRVLVE
                     ILVVLFARRLVENPGLSALVPSDAAGLKAYLRSVLSSVDLYYVWHIGLLLISARTLSG
                     LSKSKALTSALVAVFVMLLLLGIPGFIGTYLSGISTTSRFYFF"
     gene            complement(31391..31768)
                     /locus_tag="PKL60_01875"
     CDS             complement(31391..31768)
                     /locus_tag="PKL60_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HNW94914.1"
                     /translation="MFTDPKGPITRFEWARFSIHGAVHSAEEGVGKDIFLSSEGISAW
                     HERRGHKLKPGMLARALELKPEVLLIGTGVEGALEIGKKARKAASSAGVVLVAMRTPQ
                     ACQEYNRLFHLGKKVALLAHGTC"
BASE COUNT         7089 a         8980 c         8746 g         7140 t
ORIGIN      
        1 gggcgcgcgc tggagcgtcg ttccgcgtcg cattgctgaa aactacggtg cgttcacgcc
       61 gtggatcgcg ctgctgttta tcccgattgc ggtgttcgcc aaggacatcc tgccgtggat
      121 gaacatcgaa ggcatcaaga acccggaata caagacggta actgctgaaa ctgtgctgta
      181 caaagacgga aaagaaatcg cgcgcttgaa ccccgcccag catatctatc cggaccgcca
      241 acaatatatc actatccccg acgcgcacgc cacgctgcgt ggggacgtat acaccctgtt
      301 ggcggggtat gatgttgata ttccttatat caccctgcgc ctgtccgata atcccctggt
      361 caatttcatg tgggttggcg gggttttcat gacgattggt ggtgcgctgg ctgcgacctt
      421 gcccgccagg cgcggcgagc gggaagatga aacagaaact atggaggaaa cgcttgactc
      481 ttcaggcgcg gcggatttct aaagcatacg gcgttcaacg cgttctggac aagctcgatt
      541 tgtccattga agcgggggaa gtggtgtgtc ttctgggccc gaacggcgcc gggaaaacca
      601 cgctcatccg tctactcagc gggctgcaga aagcggacag cggagaagtg ctttggggaa
      661 cccaggacgg gaacttaagc gtgctcacgc tgcgccgcag catcggcctc atcctgcacc
      721 agcccttttt atacgagcac ctgacaggct tcgaaaacct gcgcttctac tcccgcctgt
      781 acgaaaccgg attgagcgat gccgatttgc gcaaaatgct cggccgggta gggttgggca
      841 gcgcgcgccc gatacccgtg cggggatatt cacgcggcat gaaacagcgc ctgacaatcg
      901 cccgggcttt attgtgcgat ccccagatac tcctgttcga tgaaccctat accgggctgg
      961 accaacaagg ctgcggcatg ctgaaccagc tgctgctgga agagggcgcc cgagacaagc
     1021 tggtcttgct gaccacgcac gagctcagtt ccatccgcca ggttgccacg cgctttgacc
     1081 tgctgcaccg cgggaagatt gccgccagta ttcccaacga cggcatgagc ctggacgccc
     1141 tgcaggatca gtacaacgcg gtcatcgcgg ctggtaagag cctccaaaag gagcaggcat
     1201 gaaggcctgg ctccactccg tgcgcgtttt ggtctggaaa gacctacttc aggaatggcg
     1261 cagtcgcgat attctcactt caatgctggc ctttgctgtg ctctcgctgt tcatcttcaa
     1321 ttacgcgctg gaactctcgc cgatcaaccg cgcggaaatt gccccggggg tcatctgggt
     1381 ggtgatggtg ttcgcgggta cgctgggttt gaaccgctct ttcaccgctg agcaggacca
     1441 gggctgtttc gaaggtctga tgatggcagt ccccgaccta tcggcgattt atttcgccaa
     1501 gctgctcaca aacctgctga tgatggtctt tctggctgtg cttgtcatcc ctatttactc
     1561 gcttctctac aatcaatccc tgtttaatca ggcgtttttt ggtgtgttga tattaggagc
     1621 ctggggttac agtgcggcgg gcacgctgat tgccagcatg accgtgcaga cccgcatgcg
     1681 cgatttgctc ctgccggtcc tattattccc gctgctgctg ccggtgaata tggcagtggt
     1741 aaaatcatgc ggcgtgatcc ttgggtccgt agccggtcag gacattcacg gctggctgaa
     1801 cctcttgttg gtctacgaca tcgttgtcac aatactttcg attatggtgt ttgaatatgt
     1861 catccaggaa taaactttta cccttgcttt ccgttctggc atccctgctc cttctgtttg
     1921 ccttcctccg ggtgctattc ttcacaccgg tcgagcagac catgggtctg gtccagaaag
     1981 ttttctactt tcacatgagc gcggcgtggg tggggatgct ggcttttttg gcggcagccg
     2041 tgttcggtct gctgtacctc tcgcgcagag acatgaaatg ggacactctg accgcggcga
     2101 gcgtggaagt cgggctgctt ttcacgataa tctgcatcct cagcgggatg atttgggcgc
     2161 gccccatctg gaacacctgg tggacctggg atccgcgcct gacaacggtt accatcatgg
     2221 cttttactta tattgccttc attctgctgc gcaggggggt ggacaatccg acccggcggg
     2281 cgcgtttcag cgccgtgtat gccgtcatcg gctttatcag cgttccgctt accttcttct
     2341 ctgcccgttt gctgcgcacc atccacccgg tggtctttgg cagcggtgag gcggatatga
     2401 acctcaatcc gcaaatgctc gggacgcttt ttatctccct gggcgctttt accgtcctct
     2461 atgcagcgct tttacttcac cgctcccgac tgatggcaga gcaggaagcc ttgaatcaac
     2521 aattggctgc ggacgacagc cactacgact ggggagaata agagtatggc ccaaactttg
     2581 aattacttca tcgccggata tgcagttatc ctgggtttac tgagcgctta ttgcctgtat
     2641 ttgtatctgc tcagccgaaa aataaaaaaa acacgcgcct cgctgcagaa agaaccgcat
     2701 gaattttcag gaaaggaatg aaaacgggtc aattgtgtct tttatcacat tgacagatag
     2761 gcacttgctt ttataattat gtatgtattt tatccaaaat atcttatata gtaattactg
     2821 gagaggaaca tgaaaaagag cacgttgttc gttttattag cagtagttgc gcttgccttg
     2881 gttgcctggg ttggctgcac taccaaagag gcggttgaag aaccagccga agaaaccacc
     2941 tcagggatca atgtagttgc agcaaaatgg gccggctcag cgcatgctga agcatcctcc
     3001 gaagctttca accattggaa tgaagaggat cctcaggaag ttccgccaac ctgcgctaaa
     3061 tgccacagtt cgactggctt ccaggattac atcggcgctg acggctccga agcgggaaaa
     3121 gttgatgctg ccggcaagat tatcgacccc atcaactgcg cgacctgcca caacgacgcc
     3181 gcagacaaac tggacgccgc gaccttgcca aacggaacgc agcttgctga tatgggtaaa
     3241 aacgcgattt gctccacctg ccacagcggc atgggttcat ctgcagacgt cgccaaaaaa
     3301 gttgccggtc tggctgaaga cgatgtcatc gccgatcagg gcataatggg tgttcattat
     3361 ctggccgcag cctccactca ggcaggcgct gacggcggta tgggcttcca gtacgaaggc
     3421 aaaacctatg ccggcacctt caagcatgcc gaacccgtga actcctgcac cgaatgccac
     3481 gacccgcact ccctgcatac gcagagtgtc cctggcagcg atgccaatct gtgctccacc
     3541 tgccattctg atgtgaagag ctaccaggat tacaagaaaa tctcgatgtc caaagttgac
     3601 tacgatggcg acggaaccgc tgaaagcacc tacgatgaaa tcgaaggcat gcgtgctgtt
     3661 cttattaaag cgctcgctgg ttattccacc gccaaatccg gcgcgacttt cggcttcaac
     3721 ccggctgctt acccgtatgc cttcatcgac accaatgccg acggcacctt gagcgcggaa
     3781 gaatctgcgt tcccgaacca gttcaaagcc ttcacacccc gcatgctaaa agccgcctac
     3841 aacctgatgt tcgtcaccaa agatccggct gcctacattc acaacgcgga ttacgccctg
     3901 cagctgatgt acgactccat cgagagcctg tctgagttca gcggtgtcag cactcagggt
     3961 ctggttcgcc cctaatctgg cgcgaaaccg ttcatagaaa tcgagacgct taaatcatgg
     4021 ccaagaaaac taagctgatt attttcgtag cgctctgtat tgcgcttact ctgaccccgt
     4081 tggtaattat gctggtcaga gccaattggg cgctgccaat ccgggatgtt tccgtcgcgc
     4141 tggcattctt tgggatggct gtcgtgggta tgcagtttct ccccatatcc cgcatcccct
     4201 ggctgtcgga ggtggtggaa ttggacaaga tgtacaagat ccatcacaca gtatctgcct
     4261 tcgcggtcct gctggtggct ttacaccccc tcatcctgtt ggtcaccggc agtgtatact
     4321 tccacggttt cctgatgatt gccggatgga ttggcttggg tggtttggtt ctgatcgcga
     4381 tcacttccat ttatcgcaag cagctcaaaa tcagctatac cacatggctg ctcatccatg
     4441 acattctcac gcttctgatc ctggtcttcg gtctgctgca tatgttccag cgcaattatt
     4501 acctgcgcga caccggcatg accatcgcat ggattatcct gatgtgtatt tggggcggtt
     4561 tggcgctata tatccgcgtt atccgtcctc tgataaatgc caggcatcct tatgccgtga
     4621 gcaaggtcgt ggaagaaggc aaagatacct actccgtcta cctcaaagca gagggtttca
     4681 agcgccctgc cttcaatgcc ggccaggtag cctggattag caagggacct tcgcccttcg
     4741 ttatcagccg caatcccttc tcctactccg gaagcacgga atgggacgag ctgcgctttt
     4801 cgattaagaa agccggcgat ttcaccaaca ccatccccga cctgaaacaa ggcgatcgga
     4861 tgtttgttga tggtccttac ggcaccttta atctcaagaa tccgcgcatg caaaaaggct
     4921 tggtcctgct gggcggcggc atcggcattg cgcctgtgat gagcatggtt aatacccttg
     4981 ccgataccgg cgataaacga cccgtgtacg tgttttacgg cgacttgtgt gaagacactg
     5041 tgctcttcgc caatgagttt gaagcgctca aggtccgcat gaacctgacc gtgattcaag
     5101 tgctcgagaa gcccctcgac gtagagaaat gcaagcacaa aggctacatc accaaagacc
     5161 tgttactcag caccctgccg gaaaactata aggaactgta ttactttatg tgcggtccaa
     5221 tgcctatgat gaacgcgatg aacaaacatc tcaaagcaat gggcgtggac ccggcccaga
     5281 ttgctactga aaagtacgag atggcctaaa ccccaacgct tgaagacaga cctcccaaaa
     5341 gggaggtctg ttctttttca ccggaaataa agcggcagcg cggctctatc tgcagctctt
     5401 gccagactgg aacgaagctg taggatactg tacaatcaca gaactgttga aacacaaaaa
     5461 ttaaaggaat ccaaatgcca aataatcccg accacgatca tattccaagc aagacgctcg
     5521 accggaaact gaattggata cttttcctgc agattgttct tatcgcgctc atcctagccc
     5581 tgtatgtttt gggcaagtaa tccaggcagt cccaccaacc gatccttcca cttctttcca
     5641 cggagccagc ttgtgcagca gcctcaagcg gtggttttcc cttgacctga aaccagctct
     5701 ctttccctgg cactgatatt gcaacgtata cgctaacacg acccgccgcg cgctgttttc
     5761 tgcacccgta cagccggcag gcgcgtcgca agagccggag gatggtatga ccagagcatt
     5821 tggtgtggat atcagtaaat atcaaaccag ccaggatgga accaagcggc aggattttaa
     5881 tgccttgcgc gctcataccg aggaggtggt attcattgcc gcgcgggcag gggtttgctg
     5941 gggctacaaa gacccgatgt ttgattacta ctggagcgaa atggcgcgca tccaggtctg
     6001 ccgcatggct tacctggtgg tgtactttgg cgaaagcgcg atttcccaaa tggacgcgct
     6061 ttttaaaatc gtggaagggc aggcaaattg gacgcatgac cgcctggtcc ttgacctgga
     6121 agtggctgga attaacaccc gcgaacgcat cacctctacc acccaaaaat gcctggatat
     6181 ctgcaaagcg cgcaccggca tttatcccat ttgctattca cgcgcctcct gggtcaatgc
     6241 ctaccttaaa atagaccagc ttccgcgcct ggattggtgg ctggcaacct acaaaaaacc
     6301 ttttccgtat cccgcgtata cccccgagca tcctggtccg cctgatttgc cccgaggggt
     6361 ggctacctac cttattcatc agactggcga taagaacaaa gccatcggct ctgccagccg
     6421 ctatatggat tttgaccgtt ggaacggaag caaaaccgac gtcttccgct acttcaacaa
     6481 ccccgataat ggcaatccca atcctccgcc gccggaccct ggcgcagtac ttttccgcgc
     6541 ccgctgtgtg gtatcagcgc tgtacaagcg cgaaggcccc ggcacgcgct acagcgtagt
     6601 tggttccctc aatctgggtg aggaggtgaa tgtttatgag gtcaaggaag actggttccg
     6661 catcgacccg caggcttccg tgtggtgctc cgggaacgcg cgctatatgc gcatgctgga
     6721 ccccggccaa cccgatccgg taaagcagcc catattcagg gtcaaggtaa ttgtcactgc
     6781 tctgtataaa cgccgtggac ccagcgccag ctacgcaatt acgggctatc tgcagaaggg
     6841 cgaagaggtt tcggtctatg aggtcacgaa taactggtac cggattgacg cagaaaacca
     6901 ggtatggtgt tccggggctc cgcagtatac ccaaaaaatc taagtttgtg cttaatagaa
     6961 aatggctgcc aaacggcagc catttttttc gtacttcgag atttagaggt cgttcaggag
     7021 aacggccagg gccaggagga cgaagccagc cagaacgagc cagaaggcat attgtgccag
     7081 cacggcaatg acgcccaact gggcaagcag tccggccagg gcaagcacga gcgcgacgag
     7141 aaaggtaacg aattttggtg cagacagttt cataataagc tccttttgaa taaattggat
     7201 tgattgtcaa aaactatctt atctgtatag gcgcggattg tcaagcaggc ggattagttc
     7261 atctgaaatt gtgacgaatt cgcattttca gctcgaacaa gcatgtttac aggctggcgg
     7321 cggctgaaat agacagtatt ttgaccttac agtattttga ccttaccata tttttctatg
     7381 catctgctga tataatccct tgactacgta aaaggatggt cccatgagtc aggattccac
     7441 tccccccagt cttccgggaa tttccatttc caggaatggt ccttacatcg ttgaaggcgc
     7501 ggttcccctc gtccgcaaaa cgcagattgt ttcagagtac ggagagcccc tcagttggca
     7561 gctgctttca cggtacgagc tgccagaggc gcgttaccga ctctgccgct gcggtaattc
     7621 caaacagatg cccttctgcg ataataccca cctgctgaaa ccctttcaag gcaaggaaac
     7681 agcctctacc gacggcagcg aggacgattc cattgtgctg ccaggcggga agcagattat
     7741 tgtgcattta taccgggatt tgtgcatgag ttcgggcttt tgcaacctgc gcaatacagg
     7801 tattgtccgt ctgacagcag aatctgagga caccctcaag cgctcgctgg caatggcgat
     7861 gatcgaacgc tgtccatccg gcgcgctgac ctaccaactc gacccggacg aacctgaagt
     7921 tgagcctgac ctgccgcagg aaattgctga gaccttcgaa atcacatccc acggtcccat
     7981 tcgcggcccg ctgtgggtga ctggttacct gaagataaaa cggtctgacg gcgtgccttt
     8041 cgcggcgcgc aaccgggtga ccctgtgctg ctgtgggaat tcccgcaaca agccgctttg
     8101 cgacggcacg caccggcgcg acgcggaatg ggaagccgaa tggcacagga agaggaaata
     8161 gacgggcaga cttaagcctg agggtgctct cgcagatacg cgagcacttc ttccgcccgg
     8221 attggcaggc ggaaaaccct caagccaatc gcgttgaaga gcgcattggc aaccgcgggc
     8281 ggggtgggaa tgctgacaat ttcgcccaca cctttcgctc caaacggccc ttcggcagtc
     8341 gggtgttcca cgagtataga tttgacctgg gggctgtccg gcatgcgcgg gattgggtag
     8401 cgcgccatcc gatccgtctc cacgacgcct tcattcacgc ggaactcctc catcaatgcg
     8461 tgccccacgc ccatgacgat gccgccttcc acttgtccct gaaaacccag gggattcaag
     8521 acgcgtccgg cgtcgttcgc ggaaatcacc tgcagcacct gtacgccgcc gtcctccaac
     8581 gagatttcaa tctcgactgc ttgcgcgctg aaagaataag caaagtgaat cggcgccggg
     8641 ccgccgagcg ggctggtctc aggcgcgtgg tagactctgc ggatggatct gcctgccggg
     8701 ctggcatcca tcagcgcggc aatctccggc cagcgcatgc tgtgcgcagc gtccggttca
     8761 tcttgggggc tggttcgcgc gccttcagaa ctgaaacgga tttgctccgg ctccgtatcc
     8821 cagcgcgcag acagattggc gagaatgtgc tgcttgagct ctttggcagc cagcagggct
     8881 gcgttgcccg tgacaaaagt ctgacgcgag gcagtggtcg ggccgctgtc gggggtgagg
     8941 tcggtatcca tcaccagcac atccacgtcc cgtgggtccc gcgaaaacac ttccgccgcg
     9001 atgagctgca gggtggtcac cagcccctgt ccgatttccg cggcagcgct gcgaacctgc
     9061 atgcggccgt ccaggcacag ttcaaggtcc gccgcgccag catcatccgc gccgccgccc
     9121 aggccagtgt tcttgtaggc tgcggcggtt ccccaacagc gcaggtactg tttgccgccg
     9181 cgggtaaggc gcgtgggctt aaagggttct gctcccgcgg cctcgcgcag cgcccgttcc
     9241 accgcccgca aacaatccgg caaaccaacg ctgccttcaa gcagctgatt tgtgttcgtc
     9301 aggctgcctg cgtgcagcgc gttaatcttg cgcagttcca gcgggtccat tcccagggag
     9361 tgcgccagaa tatccatcat actttccacc gcgaaggcgg actgcagcac gccaaaaccg
     9421 cggaaagcgc cggctggcgg gttattcgta tagacagcgt agcagtcgga cttgacatgg
     9481 ggaatgacat acggaccagt ggagtgcgtg gtcgcgcggg tcatcacttt ttcgccaagc
     9541 gaggcgtaag cgccggtatc gccaaaaagt tctgtctccg cggcaacgag cagcccgttt
     9601 ttccgcgcgc ctaacttcac ccggatttgg gtagcgtgcc gtttggggtg caccagcagg
     9661 ctttcgcgcc ggctgaaaag cagctttacc ggtcttccgg ttgcccgggc cagcaaggcg
     9721 gcgtgaatct ggcctgcaat gtcctctttt ccgccgaagc ccccgcctgt gagctgcccg
     9781 cgaatgcgca cctgttcttc cgggacgccc agcgctgccg cgacctggct gcgatcggag
     9841 taggggatct gcgaaccgca atagacttcc atgcgcccat cgggcagcgg cactgccagg
     9901 ctgcattccg gctccatgaa cagatggtcg gtaaaaggtg tgaaaaagct gtgttcaagg
     9961 ataatttccg cgtcccgaaa gccctgttca gggtcgcctt tgcgcacctc gatgtgcttc
    10021 agcaggttcc ctttctcatg cagaagctcg gcctgggggg caagcgcctg ctggggtgtg
    10081 gaaagcaccg gcaggggttc cagcccgacc tgaataagcg caattgcggc gtccgcggca
    10141 gcttgagtat ctgcggcaac cagcgccagg gcatccccca ccgtgcgaat gcgctcccca
    10201 atccctacca gaataggcca atcgctggtg aataaaccgt gcaaacgcgc agcgggcagg
    10261 tctttggcgg tgagcacggc ttgcacacca ggcagcgcca acgcccggga gatatccaac
    10321 gagtgtaaga ttgcgtgggg aacgttcgcg cgcaatacgc gcgcgaagag catcccggaa
    10381 aaagccaggt catccgtaaa aaccgccgaa ccatcagctt tagctgctgc gtccggccgg
    10441 ggatgcaggg taccgattac actggtttcc gctggaacca ggctgacctt tggggctgga
    10501 agcggaccgc ggccgcgcat gaccgcagct gccgccaaca ccgcctgcag gatggcctca
    10561 tacgtgccgc agcggcagag cacatccttt agcgcagcct tcaactcctc ataatccggg
    10621 tccgggtgtt ggagcagata ggcataggcg ttcaaaatct gtgccggcgt acaaaaaccg
    10681 cattgtatcg ctccgtagga agtaaaagct tcctgcagcg gatgcggact gcccggagtg
    10741 ggggagagcg cgcgcagccc ctcaatcgta agcagggatt tccccgcggc tttcctggct
    10801 gggtagaggc aggacttgac cggtttatca tccaccaaca cggtacaaat gccgcagcgc
    10861 ccttcgccgc agccgacttt cgtgccggtc agatgcagac gcccgcgcaa gagggaagcg
    10921 agcgtctcac cggggatttc ggggagggaa taagagctgc cgttgacttt taagtcgagc
    10981 gtggccatgc cagaattata accctgctta gccggcatct ctgggcggcg cgcagaaaac
    11041 caagtgacgg aatccttctt cccacggatt aggccgcgcg tcttgctcct gaaatcctga
    11101 cgacccggaa aaaatcgggt tatatgataa ccaatatttt cttgcaagct gtgctataat
    11161 aatcgttaca aatgatatcc ataaggagcg aaacatgaaa ggtaacacga aagttctgga
    11221 agtattaaat gatttactct cggatgaatt gaccgccatc aaccagtaca tggttcacgg
    11281 cgaaatggct gaggattggg gttatcccgt gctgcacgaa gcgattgaaa aacgtgccat
    11341 caccgagatg cgccatgctg aaaagcacat tgcccgcatc ctgttcctgg aaggcatgcc
    11401 aatcgtcagc aatctgaagg ccatccacat tggcgcggac gtccccaaga tgtttgcgaa
    11461 tgaccatgct gccgagcagg aagccatcga taagtacaac gcggccatca aagtggccgt
    11521 ggaagccggc gataacggta ccaaggaaat gctcgacgcg atcctggtgg atgaagaagc
    11581 ccacatcgat tacatcgaga ccgaacagac caagatggaa cagatgggcc tgaccttcta
    11641 cctgtccacc ctgaccaaag aagactaaat ttcagccccg ccagaatgtc tgctggggct
    11701 gaaaggttgc gcgtagcctt acacttttca taaaacctgg aaacgctggt tcaatagcag
    11761 ccgccccaaa agggcggctg atttttatgt acggatgaaa ggatcgccgg aactgggcag
    11821 ggaggaaatg agcagggtgc cttccacgcc gttgctgcgc cgcacgcggg agaggttcac
    11881 cctccacttt tcgggctggc tgcccgggga tattggcata aacccatcag gacccctcct
    11941 tttggagggc tttcacgctt cgtaaatcct cgccaccgtc gattggagcc gtcggagaaa
    12001 ggggattcat gctgaaaaga atgaactttt cggaacggtg agcctccgtg ctgcggggat
    12061 aatccgctgc gggaattgcg cggggagggg gggcgctgct tccgctgctg gtgtacacgg
    12121 cagcccgcgc acccgctatc cctccgtcct ccaggtttga taagcgcctt gcctcatgca
    12181 caagacacgc cgaacccgca tatcccttcc gtcaataata cctgtagctg tgcgcagttc
    12241 cctttcgccg tcctccggct caaagctttg gtccaggccc atagggaagc agtatggctc
    12301 gaagagattt ttcgggtaag cctctcgaaa gcctttaccc gcgaggctgg cagttccgcc
    12361 tattccacat tattttcccc ttcggaagta atgtcggttc tgaagggggt gccgccggga
    12421 atgctgcgga acagcccgat gcagacaatg gtttttcctt ccaacgcccg gtcgcaacgc
    12481 atcggctgca cattccagcg ctcttcctgg ttctcaagcc ccggcagggc gataacttca
    12541 aattcggccg taagccgctg ctttccagaa cagggttcca ggtcaaccca caggcagcat
    12601 tggccgcgcg gaaattcgtc atgacgctct tgagcgcttc gaagttcagc ttgaccccgt
    12661 tggcaatcgt ttcattaagc tctttgatag tttcgcgcag ctccgctccg atttcaatgc
    12721 ccgccataag actttcgcta atctcgtcaa cgcggtccaa cgtattccgg ccaaaccgct
    12781 ggcgctggca gccttgatac tccagacagc gcctttagcc tggtccgggt tgtattccat
    12841 ctctcctcca tgggatgaaa aaagcgatgt cttgcaagct gtttccaacg gaacagcagg
    12901 gtcgctgccg acagcggcct ttttcttgat ttggatgctg ctaatacgaa agcggaatcc
    12961 cacgaaagaa aaagaaatcc ccgctcagat tattaccagc tcgtacagcc tgatataatc
    13021 aaaccgtaag aagctctggc attccttcaa atataggtca caccaagctt accgcgtcca
    13081 cgcatgtact ttgcaaagga gaccatgatg cgcagccgct ttctcttgtt agtccttctg
    13141 actgccgcct gtctgcttgt gctgcaggct tgcggcccca ctcccacccc tcgaccgctc
    13201 ccgaccgaag aaccgacgct gccgcccagc ctcccgactg aagaaatacc gccgactgaa
    13261 accgcgacgc tggaaccaac tcctatcccc acgtcaaccg aagcgtctgc gccccccaac
    13321 ggacagattg ttttccgcta cgaggccaac ctttggcgct atttggtgga tgccggaaca
    13381 ctggagcagc tcaccttcga cgggattccg ggggattacg gcagcgcgta cgaccataca
    13441 gaaatctcac cggatggggc tgtgctggct tacaccaaag gggaaagcag ctttatgacc
    13501 gatttcacca gcggtgggtc cgcagatatg acgacctacg gcaagtttat caagtggaac
    13561 ggcgtcggcc gccagttttt cactgcgctc ggggatatga cctgcccgcc gatcgaaaac
    13621 ctggaagacc aaaacctgct gaattttgac gttatgcgct acgaccagtc caatctgagc
    13681 agcgctacca atctcggcaa catcggcggg ggtctgaagt tcgtcagtac catttccaat
    13741 gacgggcaat ggctggtcgc caacagctgc ggctgctatt ctgaatgcgg caatgagaat
    13801 ctctggcact tgcccaccct cagccagatt gactcacccc ttagcttccc catcggcggg
    13861 ctggatttct cagcggacag tacgcgcttg gtgcttgggc agtggcaaat gtacggctat
    13921 caggagagcg cgctctacgt ggcaggcagc gacctgagca gccccaaccc gatttactcg
    13981 cgcgccgata ccgcgccggt acgccaggaa tggtctccgg atgggagcat gattgccttc
    14041 accctggtcg agattgaccc ggtggaaatg agcgctacgg agcgtatggt catcctcatc
    14101 agtccgaatg gagccgagca gcttctggtg gaaagtggcg gcgcagaatt cctggcctgg
    14161 tcgccagaca gcagccgcat cctctacacc cgggaagttg gcggcgtgcg cactgtttat
    14221 ttctacgacc gggtctccga aacgcgaacc ctgctgccct tcagcatcga cgcctattca
    14281 gacggcgctg tggactgggg cagactttcc gccgcggacc tgggcttgta gaccctcgca
    14341 gctgccggtc aaaaagctgg gcgcggattc cctgggcatc tgttgctctg cctggctgta
    14401 aaccagaaac atgctgccca aaagaaggcc tatggacacc ccggaattcg agatttacct
    14461 ccctggcgcg gagcagacag cctccagtac aatgccggta caagcgctgg ctgcggtcac
    14521 gcacctgggt ataggcgctc acccggacga tctggaaatc atggcgattg acggtatcct
    14581 caagggtttc tccggagggg ataatgtctt tgccggcgtt gtgctcaccg acggcaggtc
    14641 cgcgccgcgc gcggggaagt ttgcagcctt gagcgacccg gagctggcgg ccctgcgcaa
    14701 agaagagcaa aaagaagccg cgcgcattgg caggtacggc gccctggtct ttatgaattt
    14761 tgtcagccgt gacttgcgtc aggggggcgg cgtcgttgaa agccttggac aggtgctgca
    14821 ggctgcgcgg ccccgggtgg tgtataccca cagtctgctg gacgcgcacc ccacgcatgc
    14881 cgcggctgcc tggcgcgtca tccaggcgct gcgcggcctg cccgctgaaa tgctgccgga
    14941 gcaggtcttc ggctgcgagg tctggcgcgg attggaatgg ctgcccaaac gttttcgggt
    15001 cgcgtttgac tgctccgaac acccaaggct gcaaacacag ctgcttgcgg tcttcaaaac
    15061 ccaggtcgag agcggcaaac gttacgatcg cgccattcca ggcaggcggg cagccaacgc
    15121 caccatgaac gagcctgatg cggtagacca aacccgcgcg ctggcatttg ccatggactt
    15181 aaatccgctt gtgcgcgaca aggcgctatc cgcgcgcgca tatttgcggg aaattttgcg
    15241 ggatttcgag cgggaagcct tgtccgcgct gccggatgat gcctgaagtg cgccggcgct
    15301 ttgggtgaaa attcatcgcc tgaaagcaga gctgacttct tcacaggggc gatggacaga
    15361 aggctgcttt cctgttgtgc cagctggcag gtaatctatt ggaatagttg atagaagaac
    15421 gctggaaccg tgaaagctcc aagcttatag acgtgtgcca gaatcaggca ggtcgcggtc
    15481 agcaaaagcc cgaacccgat caccgcgtaa tcccaccaat ggtatttgag ctcgaacagc
    15541 caggtgcgtt tgcgctgccc gaagccgcgc aaatccatgg cgttagcgat atcctcgctg
    15601 gaaaggatgg cgttcatcgt caccggaata ataagcggcg cgattcggcc gattttctgg
    15661 atgatgccgc ctttcacatc gtccagttcg aaggcgcggg cgcgctgggc atccagagtg
    15721 atcgagaaac tgcgcgccag gctggggatg aagcggatgg cgagttcgat tgtgtaagcc
    15781 agcttgtctg gcaagcccag cttgctgaag gtggcgccgt aggagcgcga gtccatcgtg
    15841 tagggaatca ggataaaaaa catggagatg ctgccaatgc gcatcaactg gcagatggca
    15901 aaccagatcc gttccacggt caggcctggt ttcagcagct ttccgaacag gcgcagaccc
    15961 tccggccaga ccagctttcc accttcgggc accaccccgc ccgccccacc gccggtgatg
    16021 atagtattga cgacaatcat ggtaaaaagc atgattaaaa ccagcgtcca accgcggtgg
    16081 gtttctttcc aggtggttct ccccaggctg taccagatga tggcaatgag aaacagaatc
    16141 gccaatatgg cgctgttcca ggttgaggtg gcggccacca gaacggcgaa ggagaatatc
    16201 cagcgcgccc gggggtcgat gtgatccatc gggctgccgc ggtatttata atgccagctt
    16261 acaagcatga cgagttaaaa aagggggcaa gcagccgcag ccgcctgccc tgggaatcag
    16321 attaactgcg cccgctgcgt tgggtaatgg cagcgtatgc gatcatcaac aagggaacca
    16381 gtaccaggcc gttgagcaca tcagggatga aggccggaat gaagttggcg aaaatgaccg
    16441 tcttcatatc cgccccggaa acccacaatt cggataagga tgccacgccc atgccagccg
    16501 cggcgccgag caccacgaac agttccgcca gcaggatgga tttgccggct ttgtagtcct
    16561 ggatgagggg gtagataagc cccggaacca gaccgagcag accgttgcca agatcccacc
    16621 agaaccagaa gccgtagcca gagagcaggt ccgccaggat attgccgacg aagccggcga
    16681 tgaaacccac aatcggaccg aacgcgatgc caaaaaacat cgggataaca accgcgggtc
    16741 tgatggagac atttccagcg ccagggagct ggattatatt ggtgacgtac gccagtacgg
    16801 catacagtgc cgcgccgatg gcggcataaa caacttgctt cgtaccgaat ttccacagag
    16861 acttcttttc ctgcatacaa aacctccttg ggcatggata aatgaatatg acagaaccat
    16921 tttacccgat tccatgcgag caagaacctt cattttggtt tgagcccttg ctctgttcac
    16981 ttcttataag ggaatcagca aggtctgcat cctggcagcc tgatgccctg gtgggtacag
    17041 gtcatgagcg aagggtcttg cccgccgatt tggaaccgga tgatgccttt gcaatttacc
    17101 gaaactctaa tcgatccttc acttcgttca gaatgaccag aaatgtcaac ctgagcagcg
    17161 cggtgggtct tgccccactc tgccgatcag attgttctct gtgttccgta tggcaaaaga
    17221 gcattacccg atatagcgcg caagcgcttc cgcacgctgg aatttacccg tccgcgggaa
    17281 catccggagg ttcaactcga gcagactggt aggcagcagc cgccactgga gcagctgctc
    17341 actatccttc aggacttcct gcggcgtgcc gtccgcgaca atccggccgt caaagagcac
    17401 aaggatgcgg ttggcatagg tcagggccag gtcaaggtcg tgcgtgatga agaggatgga
    17461 atcgaagccg ggcatttgca ggattgtatc cataaaggat tgatagttcc agtaatcctg
    17521 cccggcggta ggttcatcca tcattagaat gcgcgagcgc atggatagca cggaggcaat
    17581 gctgacccgc ttttgctgcc cgaacgatag cgccagcggc ggagtgccca attcttcttc
    17641 aaggtgcaca gattgaacag cccaatccac attcttctgg attatctctt tcgaaaagcc
    17701 cagattttta ggtccaaagg ccagctcgtc ccgtactgtc gcggcaaaaa gcatctgcgt
    17761 cgggctttgg aatacatacc ccaccgttcg ggcggctttt gccacggttg tggatttgga
    17821 ctcgatgcct tccagcagca cacttccgcg cgtaggcttg agcaaaccca gggcgtgttt
    17881 gacgagtgtg gttttgcccg agccgttgtg cccgaggatg gcaatgactt ccccggtatg
    17941 gatattcagg tttaccccat gcagcacctc aggcaggtct tcgtggtagc ggaagcctac
    18001 ctctttgaac tgaatcaagg gatgggaata atccagcggg ggaacggccg gcacaaattc
    18061 tgtttcgggc gcgtctttag ccctggcaag gatgtccgct gcaggcagtt taatcttgtg
    18121 gtaatcgacc acctggctga ggccggcggg tccaccttga taggtaatct caccgtcttc
    18181 catatacaca acgcgctcgg ggtgaatcgc gagcacatct tccacgcggt gttccacaat
    18241 catgactgcc agaccatcgt ctgccagctg ccggaaggcc agcagcgctt cgcgcgcgga
    18301 agccgggtcc aggctggcga gtggttcatc gagcagcagg attttcggct gcatggccaa
    18361 tacgccggcc agcgctactt tttgcttttc cccgccggag gtcccaaagg tctctttgtc
    18421 gcgcagatgc agaatgttca gccgccgcag tgcgtcttcc ccgcgccgga tcatttcctg
    18481 gcgttccagg cccaggcact ccaacccgaa gcagacctca ttgagcacgt aagtgccgag
    18541 gatttgcctt tccgggtcct gcagcagcgt tccgacgttt tgggagatat ccgcaaggtt
    18601 tttcccgcgc aggctctcgc cgtagagcag gagttcgccc gtcatttcac cgtggtaggc
    18661 ttcggggata agtccgttga tgcagcgcaa cagggtggtc ttgccgcatc cgctggaacc
    18721 agccagaagc agcacttccc cggggcgcaa ctccaggttg atatttttga tcgccgggat
    18781 gctgcgcgta ttgtaacgga aggacaggtc ccggatgatg agcggcaaaa cctccggttc
    18841 agtcattcag gctgacctca acgatgtcat caatttgcgt ggaaagagca tttttcgcgc
    18901 tgccgttcac aaccgcgatt tcgatgtagc cttcgctgtc ctccagggcc accaggtcgc
    18961 cgggctgttt gtggccgtag gagagcgcca gcccctcaat cgttcggccg cggatggaaa
    19021 aggtgacatg tgcgtggttt ctcacccggc tcgcgggcag gtcggtggta aggttgccga
    19081 acacatccgc cagcaggata tgcgcacgga agccattttc tgtttgttcg gccgcgggca
    19141 ggggtttgcg cataatatcg gtcacgggcg tgcccagttc ggataaggct acaccgtttg
    19201 ccaggtgcgc cccgcacggc gcgaaaacgt cgcgcccgtg gaaagtgcgg cttacgttcg
    19261 gcagccaaaa gcggggctga tcgaggcaaa caacctggac ggattcaccg gcggcttcgg
    19321 cttcctccag catcggcgtc agcaagccgt tatccggcgc gatgaagaac tgatttccaa
    19381 agcgtgcgcc aatcggccgg cgctgtgtgc ccacgccggg gtctaccact gcgatgtgca
    19441 cggagccggg cgggaaaaaa ggcgcggcac gccacagcgc gatggcaccc tggcgcacat
    19501 cctgaggggg aatatcgtgg gtgatatccg cgatctgagc gcctggggcg atgctccaga
    19561 taaccccttt gagggtgccc acaaagccgt tcagattacc aaaatccgtg gtgagggaga
    19621 taatcgacat gcgctaaggt taacattccg gctggaaaac gtcaagaaaa tccacccctt
    19681 gccgccttcg aagttcaccg ctccggccaa gccaatccgg aaacttcaat cagggaatag
    19741 aatgattagc gccaaagttt tgccagagtt tctattaacg ttagattaaa tcttgacaaa
    19801 cataagattt gtattaaaat agttgatatg tttactaaat gcattcgcgc ttagtcatca
    19861 gggaaatggc accggaacgc atgatttttt acgaatcgtt gaaggcaatt ttccttcata
    19921 tcgacaacca cgagaaggct tttctgagcc gctataacct gtcgataccc cgcttctacg
    19981 ccctcctgca catctacaac caccccggca taaactacat cgagttgagc gacctgatgc
    20041 tctgcaccaa gtccaacacc acgcgtgttg tacaaggcat gcagaaggac ggattggtcc
    20101 tgcgcaaggc gcacccggcg gacaggcgca gcttccagct ttcgctgagt aaatcaggga
    20161 gcctgctctt ccaggaagtg tatcccgaat atcttgcgct cattgacagg ttgatgtcgc
    20221 tcttcagcga cgaagaactg gaacgttatg ctgccgcgag caatcacatt gaaaacaccc
    20281 tggctccaga aaacctggcg aaccacagag gcgcgctgcg cgtggagaac ccgcagcccg
    20341 tttcaccgct cctcaattga ggtcaggatg tccactaaaa ccggcaaaca cagccaaatc
    20401 attcaactgt ttcaaaggag aaaccaatga agaaaagtgt aatgcttgtc gcaatcttat
    20461 tgattgcctc tctggtgctc ggcgcttgcg ccaaggcaac agaggcccct gttgtgaccg
    20521 aagccccgac tgaggctccc gtcgttaccg aagctccgac cgaagccccg accgaagcat
    20581 ccgccggtac cctgcgcatt tgggctgacg aacagcgcgc ccccgttctt gctgaattgg
    20641 gcaaacagtt caaagccgag tacaacgtgg acgtggtcgt cgagaatatc tccggtattc
    20701 gtgaccagtt caccgttgcg gctcccgctg gcgaaggtcc cgatattatc gtgattgccc
    20761 atgaccagat gccggcgctc gttgcctccg gtctggccgc cgaaatcgac cttggcgcca
    20821 agaaagccga tttccttgaa tccgccatta aagctttcac ctatgcaggc aagctctatg
    20881 gtatgcccta cgcccaggaa aacctcggct tcttctataa caccgagctc gtccccaccc
    20941 ctcccaccac ctggcaggaa gttcacgaca tcagccagca gctgattgct gacggcaaag
    21001 ttgagtacgg tatcgtgctc ggcggtactt cctacgatgc atatccctgg atgacctctc
    21061 agggcggcta catcttcggt ctggatgcca atggtgacta caatcctgcg gatatcggcg
    21121 tcggcggtga aggcatgatc aaattcggcc aaatggcgct ggattgggtc aatgacggca
    21181 cgctttccaa caacaccgac aatactactg ccaaatccat gttcaaagac ggtaacgtgg
    21241 ctttcttcat gaatggcccg tgggaaatca agaacctgcg tgaagccggc actcccttcg
    21301 ccatcactaa gttccccgat ggcggaacgc ccttcctcgg cgtccagggc tttgtcgtga
    21361 atgccttcag cgagaacatc ctgcttgcac agtccttcct gactgaattc gtcgcgaccg
    21421 aagaagcgat gctcaagctc tacgaagccg atccccgtgc tcccgcctac aaacccgcgc
    21481 tcgagaaaat ggatgatccg gacttgctcg cgattgccga agccggcgaa ggcgcactgc
    21541 cgatgcccgc tatccctgaa atgggcgctg tctggtccga ttggggcaat gccctgacct
    21601 tcatcttcaa tggtgaaaag acccctgaag ttgcctacac cgatgctgcc aacaacatca
    21661 agatcgcgat tgcctccaag ggcatggtca atgtgcctgg ttcctaccag accaaagttg
    21721 gctgcgcctc tgactggatg ccttactgcg ctcagactgc gctggtcaag ggcgacgacg
    21781 gcaaatacac cggcactttc ctgatccccg ctggcgatta cgaatgtaaa gtagctctcg
    21841 acggcggttg ggctgtctcc tacggcgacg gcgatgccaa ctacaagttc acggttgctg
    21901 ctgacggcaa tgtaaccttc atctttgatc ccgaaaccac aaccctcgag atcatcgttc
    21961 catagtctgt ctctgaagta acgaggagac cgggtttccc tggtctcctc ctgacaattt
    22021 aatatcgtgt atgttgcgct tcacacagtt cctcgcaaca tatcgcatgc ttcgcaacac
    22081 atagtgtgct tcgcaatgaa aggagtgaca ggtaatgggt gaaacgacta tcacgaaagc
    22141 taagtttgca aagcgaacga tttctggtgt ttcctcaata atcactaccg caattgtcct
    22201 aattgttgat gcgctccttg gatacttcac ctatcgatta ttttccctgg ggtatatccc
    22261 gctcgggata gtattcgtat tgatcatcgc aattatcagc ttcgcctttc tggttccaaa
    22321 agctcatatg ttccgctgga tggctgtcgg tttggctgcc tggcttttat tctccatctt
    22381 ccccatcctt tacaccatct acaatgcttt taccaactac ggtgatgcac acctgatcac
    22441 gcaaacacag gccattgccc agattaaagc gcagacctac ctgcctgaaa caggtaaggc
    22501 ttaccagtgg gtcgcatatc gttcgccgga aaataattat ctgctgtggt taaaggacgc
    22561 tgaaggcaat accagcattg tgcggatggt ggatgccaag gcggatgacc atacgcttga
    22621 ggtggtaccg gggcaaaacg ggattgggga acttgatgag gaaggcgcgc cgttaacgat
    22681 tgaaggctat acgcgcttaa accgcattac tgcggcgacc gataaaaacc tgaccaacat
    22741 caagtttggc aacccggaaa agaccatcca ggtgcgcagc ccatccgaag cagctgaact
    22801 tctgcccctg ttcgtgtatg acccggatac caacacgttt accaaccagc aaaccggggt
    22861 cgtttacacg gatatcgaag gcacttatac tgccaggaac ggcgacaagc tcatccccgg
    22921 ctactccgcc gtggtaggca cgaaaaactt cgtgcagttt gttaccgctc ccgggctgcg
    22981 cggacctctg gcgacaattg ttgtgtggaa cttcctcttt gccttcatga gccttttcct
    23041 gacgtttagt gtgggcttgt tgatttctat tatttatgga gatcccaact tcaaaggcaa
    23101 aaaactgctg cgctcgctgc tgttgattcc atataccatc cccagcctga tcaccatcct
    23161 gatttggcgc ggtatgctca atccggatct tggtatcgtc aaccgcatcc tggaaagtgt
    23221 gttcggggtc tcccctgatt ggttcaacgt tccgtggttg gcccgtacag ccatcctgat
    23281 tgtcaatacc tggttgggtt atccctattg gatgctggtg accagcggtg ctttgcagtc
    23341 cattccaagc gatatttatg aagccgccga ggtggacggc gccacaggct ggcagcgctt
    23401 ctggaatatt accctgcccc tgctgttggt ttccgttggt ccattgatga ttgcttcgtt
    23461 catcttcaat ttcaacaatt tcaacctgat ttacgccttt atcaacggcg gtccgcccat
    23521 tccgacggct acgacccagg ctggctacac ggacatcatc atcagttacg tgtacaacct
    23581 ggcgtttgcc agcggccgcg gcgtccagta cggatatgct tcagcaatct ctttggttct
    23641 gttcgttttg attgccatca tgtccctggc tcaattcagg ttcacgaata tgtgggagga
    23701 ggttggagaa aatgtctaat aagccccaat ctatcaaacg cagcaaacgg gcagggctga
    23761 ttttgcgctg gctgcttgcg attgtcctgg tcactttcag cattttcccg gtgctctgga
    23821 ttgtttcagc ggcagtcaac ccggtgactg acatggcaaa ccagcagctt attcccgcca
    23881 acgcagacct ttccaacttt cgggagctga cgaccaaccc ggtatttccg ttctttacct
    23941 ggctgaagaa ctccctgaaa gtttcaattc tatccacact gctgacgctc tccctgacga
    24001 ccatggcagc tttcgcgttc tcgcgtttcc gcttccgcgg caggcagggg ctgctaaaag
    24061 ccattttact tattcaaagc tttccgaacc tgctggcaat cgtggcgttg ttctcgattg
    24121 tcaagcaaat cggcgatgtc attcccgctt tggggctgaa cacgcacgcc ggtttgatcc
    24181 tggtatattc tggcggcgcg atgggcatga atatctggct gatgaagggc tacatggata
    24241 ccatcccgcg cgatatcgat gaatccgcgc atgtggacgg cgccacggat tggcaggtct
    24301 tcagcaagct catcttgccg ctgctgcgtc caatcctgac ggttatcggc attctcagct
    24361 tcatcggtac ttacggcgaa tttattatcg cccgcacgct gttgacgaaa cgcgagatgc
    24421 aaaccgcgat ggtgggtctg caaatcttca ccgccgggca gtatacccag aactggggtg
    24481 tgttcgctgc cggagcgctc attgcggctg tacctattat ggccatctac cttatcctgc
    24541 aggatcagat tgtgggcggc ctgacgcaag gtgcggtcaa gggctaaatc cacagccaat
    24601 tctttaagcc ataacaggct ggatccacat ccagcctgtt ttcttttgtc ccgggatgga
    24661 gatttcctgc atggtctccc ccgcgcattc ggtctctctt cctggcctga gaagaagcaa
    24721 gcttcgcgcc atctcgaaaa cgcttgtttc ctcagccgga cgcctgaggc acaccagcat
    24781 gcaaggctcc aaaaatggat taaccgacga ttcagatagc ccggatggta aatttaatcc
    24841 gcattactat tgctttttat gttggggagt actactatat agatatccat tcactcgaca
    24901 aaaacctgtc cttcaggact attaattagt ccgcgtcttc gggaacttaa cataacgatt
    24961 caggcgtcca gcaagtatta atcaatacta ccaaggagca gatcatgaaa atcaggctgt
    25021 ttgttattgc cgctctgata ttgaccctga tgaccagcgg cgtgagcgtc agtgccgatt
    25081 cagcggtcga tgcggaaatc tacaatacct ggagcgtcgc cacaatcgag gaaaatgctg
    25141 atgaagcggt taacatttcc acaatttttg ttggtaaacg cgaaatccca atgatgatgt
    25201 atgtccagga aaaccataac gctatctatt gggtctggga ggccacggaa gattcgccgg
    25261 gtaattgcgg gccggacaat gcctggcact gcgattccac cgcaacaaca ttcccattca
    25321 tgaatatcag caatctggcg actgaaactt acccggatac cttttcggtt ggttattcat
    25381 acgccgctgg tgggaatctg tggggaaaca cgagagagta tacccacagc atggaattta
    25441 tcagtagtga cagcgaagtt ttgctggact ttggggaatt tggcgaaact gtagcgttgg
    25501 ctggtccccc ttcgattgcg aagatcggtg gtcacttccg gggtgcggtg acactaaaag
    25561 tcggtgtcga tttcccagtc tacaagttgg tgtatatcta tttcaaaggc ggttccaacg
    25621 attcttgtag gtcgggtgtc agtagcggct acgactgtat tgtcatcgag gaaactgctg
    25681 ccggcggctt gatgggagcg ccagccctga cttccaaaga tggcacgagc aggctcctgt
    25741 attataaaga taatgccatc cgttatgctt atccctggga aaccgctggc ttgaagccga
    25801 gcaactgtgg agagacgggt tttacctggc gctgcatcga cctggacgcc cctgcatcac
    25861 ccactaccgt tggcgcaaaa gtagatatgg ctgttggcag ggatgcaagt gatctggcgg
    25921 ctgcgtatac tctcaaaggc agtcccggct cggactatga tagtctgatg cacgccagtt
    25981 acgtggggtc gggcgggaac tgtggaacag ataaaaactt tcttggggaa aacattttta
    26041 cctgggattg tggggatgtc gataactgga ttgatagcat cagcgacacc aggttccaca
    26101 tcgcttttga taaccgcaac tttccggtca tatcctataa caataagtta attgatgatg
    26161 cttccagcag acagcggctg tacgttgctt attggcccgg gcgcgtgggc ttgcctggaa
    26221 atggatggga ccagtacctg gtggatggaa acgaccagtc caccaccggt tactacagca
    26281 atatcgcttt cagcaacaac ggcagggtta tgattggtta tcttcagctg ttcttccaat
    26341 ggaatatgat catcgatgat actccgaata tcaagatcgc tacgcagtgg ttttcgcata
    26401 cctgcctgcc gttcgtcgtt aagtaaactg gttgacccgg tcatcagcgg atttgctgac
    26461 gagagttcct aaaatttgcc cggcagcaga acgcagtcga cgttgcggaa ttcatcatca
    26521 ggaagaaatg aaaggatagg ccgcaaaagg gcgcggtcta tccttggttt gtgcctgctc
    26581 gagtcttagg ctgaaactcc cttgggggtg tgctggctga attgttgcca gtttcgaggt
    26641 cctgcctcga agccggaagg gaggacgggc aattctattc gtacttcagt gcgtccaccg
    26701 gtacgagagt tgccgcgttc agggccgggt agagcccgga gagcaagccc accagcgtgg
    26761 caaacacaat cgagaaaatc gggagccaga agggggtggc ggtcgcgatc ggtgtggagc
    26821 tcatgtatcc gttgctggca gcctggctgg cgaggtaaga gctggcaaaa acgttaatca
    26881 ccgcgctgat aatccagcca agcgctgccc cgccgacccc gccgacaaaa ccgataccgg
    26941 ccgcttcgcc caggaagatg gtcaggatat tgttgttcgt cgcgccgatg gctttcatga
    27001 tgccgatttc gcgagtgcgc tccagaatgg acatggtcat cgtgttcgcg atgccgatcg
    27061 cagccaccag cagcgcgatc gctccgacgc cgccaaagat aatctgcatg atggtgaaaa
    27121 aagaattgat cccttgcacc atggcctgcg gggtataggc ctgcaaaccc atgttgatga
    27181 tttgttcgct gatcgcgacg acttctttcg ggctttccgc cttgaccaaa acctgattgt
    27241 agccttcctt ctcgcggttg attttccggc ctatgcccca ttcattccag cgggtcagct
    27301 catccagggt catatacatg ttgtagtccg actcgccttt gctttctttc agcacgccga
    27361 ccacttccat gttgtaagaa cggcgggtct ccgtgccgtc ctggttccat ttgattaagg
    27421 ttaccttgag taccttgccc atcaagtccg gcggctcgat ggggtccgcg ttgggcctgg
    27481 ggttggggtc gtagaagttg cggttgatgt acgccccgat gaccagcttg ccccgcccga
    27541 gttcggtagt gccctgggaa gcttccagat tcatatcagc caggtccgcc acatccacgc
    27601 cctggaaatt cccccaggag gtcagcttgt caaaagtaat ttcggaaccc acggcgatat
    27661 agtcctgaat gatgacgcgt ttgacgcctg gaatggcttt gatctgctcg attactgagg
    27721 gggtaatcag aacgagttcc tgttggtcag agccatttcc tccgccgccg ccgcccccac
    27781 cgccgccctt tatctccatc ggataacccg ggtagaccgt gatattggat aagtcactga
    27841 tgccccacag ggattcggtg gcgttctttt gcaggcccgc gccaagagag accagcgtga
    27901 ccaccgcggc ggtgccaatg actaccccga cagcggtgag caggacgcgg cccttcctgc
    27961 gtgccaggtt agcgacaatc aggttgacga gatcactaaa tcgcatacaa atccttcacg
    28021 tgctcactta atcagaaaat gatgtgccgt gttttccacg gcttagggaa cgttcttaga
    28081 gccttcttcc atgggcactt ccacaggttg ttccgtgctg ctgcctgtgc cgatgcccaa
    28141 aaagcccttg atcacgttcc agatctttgc ccagaagccg accggctgtt caggctcaac
    28201 cggcatctcg ccttctccgg gaagcggttc ctgcgggatt tccatgggcg gttgtacctc
    28261 gatctcgagc gcagtttcat acacgcgcag ctggttgaag tcatcggtgt aattgattga
    28321 cacgtgaatc tttgtcggtc cctcgccaaa aggcgtgtac atcacgtccc aggtaaagta
    28381 accgccggga tccaaagtac ctaccaggct ggtgttgttt tgcaggtcag cgttctcgga
    28441 gctgacggtc atgtttccaa gcacggtggt tttgcgcgaa aggttggtaa tctggatggg
    28501 cagcgcggtc atcatgccgt tataaaacat gcctggatcg cggtagaagc tgacttccag
    28561 ttgggggagg gcatagacca gcagggtaat caactgatcg tccaccgcgc gggtgccgcc
    28621 cggggcggtg tagaccaggg agactttcag cgggtaagcg ccgggcacgg tgctgacgtt
    28681 cactacaaac tgctgctcgg tttccacgct tgcgcccacc ggcagctcac ccagcaagac
    28741 cacgttagaa ctgcccacag gggcaaaatt ggtcaggtcg ccagtggagc cggagatgcc
    28801 cggttggggt gtgccttgtt cattgggggc aagcgaaacg ccgccgccgt acaccagcga
    28861 cacgttgctg gcgtccgcca cgccggcgtt ggcaatcttt agcttcagct taaaagctga
    28921 gccgggctgc aggggatcca catccgtgct gtagccggag ataaccagct gcggcttggc
    28981 ggcagcgcct ggggtagagg ttttggtcgg gctggccgcg gccgactggg caatggcgac
    29041 gtaaaacacg aaggaggtgc tgtagctggt gccggcgtcg tccgtgtagg tagccgtagc
    29101 gttaatcacg cctgaatatt tccagctact gtaatcgctc acgcgaaagc gcaatgtggt
    29161 gccgggcgca tcgtttatct cgatgctttc cagggtaaac actcccccgt ccaacggaac
    29221 aaagtcttcg ctgctaaagg tcattaccac gtttctggca gctgcatggc tgttattctt
    29281 gatcacgaag gtaaagttga aaggcgccca gggtgaaacc gcactgtttg gtcccggaga
    29341 gtaagaatca atatagagga gaggctgcgc tgtttgcgcg gcggcgggct gtaaaggcgc
    29401 gcatgccagc aggaacacca acaagagtac cgctgcgcta aatctgtaga gagcttgatt
    29461 catcgtgatt cactaccttc ccaagtcatt ttttcgattg attcgtgata ttgtgcttca
    29521 cttacctgct tgccatccag cagaaaaatc ttatcggtgg ccacgctgag catgcgcggg
    29581 tcatgggttg ctaccaggat ggtcttgccg cctttttgca ggtccacgag ggcatgcatc
    29641 acagccgtgc cgctgctggt atccagatta ccagtcggct cgtcagcgag gatgagcagg
    29701 ggattattga tgagtgcccg cgcgatagcc acgcgctgct gctgtccgcc cgaaagctcg
    29761 gtgggtttat ggttaaagcg gtccgccaac ccgacttctt cgagcttttc ccgggcgcgt
    29821 tctttgcgca ccgaagactt gatgccggaa aaacgcagcg gaaaaccaac gttctcttcc
    29881 gcggtcatgc tcgacaccag attgaaggat tggaacacaa acccgagttt ttcccggcgg
    29941 taagctgcca gagcgttttc atccagcgcg gcaatctcct cgccatccac gcggatagaa
    30001 ccggaagtag gccagtcaag tccgccgatc aggttcagca gggtgctctt gccggaaccc
    30061 gacgggccca tgataaccgc gaaggaaccc tgtcgaattt gcaggctaat cccatccaag
    30121 gcgcgcacgt ccgtctctcc catgcggtaa tgctttttta aatcagcggt gctgacgaaa
    30181 tcatcactca tatcaactgc cattagaaga aatagaatcg gctggtggtg gaaataccgc
    30241 tcaggtaagt cccgataaac ccggggatgc ccaacagcag cagcatgacg aatacagcca
    30301 ccagcgcgga ggtgagcgcc ttgcttttgg acaatcccga aagggttcgg gcgcttatca
    30361 gaagcagccc aatgtgccag acgtaataca ggtccacgga cgaaagcacg ctgcgcaggt
    30421 atgccttaag gccggcagcg tcggaaggga ccagcgcgga aaggcccgga ttctcgacca
    30481 gccgccgagc gaaaagaact accaaaattt ccaccaggac gcgcaaaatg aaaggagtca
    30541 tcgcccaggc taccagattg ctggacctca cgcggctgga acggctgccc cgcagcgtga
    30601 ttgccaggtg cagcaggctg cttgtgagca gccagatcag ccaatacccg cttacccggc
    30661 tcaggaccgg aaaaacgtat agaaacatcg gcgaagtctg cgaagcctgg gcttgtaaga
    30721 aggcattctg ctcgtcgctg gtccagtact ggaaattctc cgggatgttc gaccccgact
    30781 gaatgatttg gcgttgaatt ggcgcggaaa ccaacacagc cgcgataatc aacacggaaa
    30841 gcaccagcaa aggggtcagc cagaccgctt tatcctccgc caggacggcc tggacggttt
    30901 tgcgcggttt tatcagcatt cccggcagcc agcgccaatg catttcaggg gcgggggttt
    30961 tcctcttctc aattgtggtt tcagccatgg catcctgtct tttccgtaat gttattctca
    31021 ggacgaaaaa ctgcgcgctt tgttcccaaa gccggcggtg agttcttatt cagtagtata
    31081 aacaaaaatg cgtgcttgtg tgattgagct gaacaaacct gagagaaggg aacggttaat
    31141 tcagcgcttg gatgggtgtt gtcctacaaa ggtcttcctt ttcccgaggt taccaaacgc
    31201 cctctttctt ggtgagggga tagggctggc catatccctg ctaatcggga atgctatgcc
    31261 tgaatagcag gaatgggcta tgctgagtag acgtcagatt aatgaaacca cctcaggaat
    31321 gacaccaatc ccgctgcgat ttttcgtcta aacggggaat gggagaaaaa ttatggtgca
    31381 ctctctgctc tcaacaggtc ccatgcgcca ggagcgccac cttcttcccg aggtggaaca
    31441 ggcggttgta ctcctggcag gcctgtgggg tgcgcatagc caccaagacc acgccggcag
    31501 aagaagctgc cttacgggct ttcttcccga tctccaacgc gccttccacg cccgtgccaa
    31561 tcagaagcac ttcaggctta agctcgagag cgcgcgcaag catcccaggc ttgagtttat
    31621 gccctctgcg ttcatgccat gccgaaatac cctccgaact gaggaagata tctttgccaa
    31681 cgccttcttc ggcgctgtgc acagcgccat gaatgctaaa ccgcgcccat tcaaaacgag
    31741 tgataggtcc tttggggtct gtaaacatgg gggcctcctt ttcgcagggc agcgcgcatt
    31801 ctgccctgga gctgaaaacc gattttcatt tccattgtaa ccactccagg cgtattttca
    31861 atcaacgggc tttgtttggt gcccggaacg tagaaaccca ccggctgcca ggcggctttc
    31921 ccgcttgata cgggctccgg gaaagcgcgc gcgag
//
LOCUS       DAOQAD010000011        31700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_1350_length_31700_cov_5.822310, whole genome shotgun sequence.
ACCESSION   DAOQAD010000011 DAOQAD010000000
VERSION     DAOQAD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 31700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31700
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1350_length_31700_cov_5.822310"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..729)
                     /locus_tag="PKL60_01880"
     CDS             complement(109..729)
                     /locus_tag="PKL60_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94915.1"
                     /translation="MKTRKCSIVLIVMASIVLAACAPTSPTAVVPTNAPTTVPSEIPV
                     QAAEVETSPEESLKNGTYFIDNQPITLVNGVSETELAPGSASKLVTRFFGNQVEIDLN
                     ADGLLDSAFLLQQENGGSGIFYYVVAAIKTENGYLGTNAIFIGDRIAPQSTSIDPNNP
                     SQFIVNYADRNANEPMSTIPTIGVSRTFKFENGSLVEVAAPPTQTP"
     gene            1012..2367
                     /gene="ablA"
                     /locus_tag="PKL60_01885"
     CDS             1012..2367
                     /gene="ablA"
                     /locus_tag="PKL60_01885"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNW94916.1"
                     /translation="MSNSPFLSKRAPVYADIPDEKWNDWHWQLTHRLNTIEDFEALFP
                     LTESEKQALSSPGLFRVEVTPYFASLIDPENPFDPIRRQVIPTARELQSFTGMMEDSL
                     AEDMHSPVPGLVHRYPDRVLMLITNQCATYCRYCTRGRLVGDPRQGFMRDFEAQIAYI
                     RRTPQIRDVLISGGDPLTLSPEHLERVLSSLREIPHVEIIRIGSRVPVFLPQRITPQL
                     TALLEKYHPLWLNIHVNHPNEITAELAEACDRLSRAGIPLGNQSVLLAGVNDDPYLMR
                     RLVQDLARIRVRPYYIYQCDLVAGAGHFRTPVSKGLEILEALRGHTSGFCVPTYVVDA
                     PGGGGKIPVMPNYVLSSSPDKVVLRNFEGYISAYSEPLDYQPPDKSLTDPYKPREEDL
                     QEGVLGLLQGSQMNIKPEGFDALHMRGGELHRLRKDAHKWKALGIGHQEAGEENPQAG
                     L"
     gene            2582..3301
                     /locus_tag="PKL60_01890"
     CDS             2582..3301
                     /locus_tag="PKL60_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94917.1"
                     /translation="MNPSQTNPHAFCIKADIIGSRGSRQAQLLPDVARELNARFASGL
                     LTPFTVRAGDELFGVLINLETGFQAYKALYSLSTARGLPFYVGMGAGSVDLTSSADAE
                     LVNGEAIWLAAEALEQLKGKPDTELLARQKRARFRYAIAVSEDPAENQPLQHFLFYIM
                     SRVSRRTALQQQAAALKEAHPDWDNLRLYRALEGKEQEVIEAENAIANFSKLLRRGDY
                     QLVRDAEEAFLFLIRRFCCGG"
     gene            3351..4193
                     /locus_tag="PKL60_01895"
     CDS             3351..4193
                     /locus_tag="PKL60_01895"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HNW94918.1"
                     /translation="MIKPLAILILAHLLADFILQPASLIALKQNKNRPPKFWNSGIFW
                     HSVVHLAVSTALLVLWGLWGWQAGLAVLCIFTAHYLIDRFKFLWKFQGTWVFILDQAL
                     HFLAIYLILLAFGLAAGPIPLVGLAVSFGQHSLPLSLSNKLALGAICVIGLVWVSGHL
                     VKNILGSLRLRSFESLKKAELENSAAPEPGNSPTQTEIRDAQPAEASTGLYIGYLERL
                     LVGVFMVSGIYTGLGLLGTLKTLARFKQLENREVTEYFILGTMLSMLLGMLFGALLRF
                     IILY"
     gene            complement(4205..4855)
                     /locus_tag="PKL60_01900"
     CDS             complement(4205..4855)
                     /locus_tag="PKL60_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNW94919.1"
                     /translation="MAIITMSRELGTGGGLISQMVINSLNYHLVNKEIIGKVLAQFGM
                     LSFNKVYDSTHNVWSLFDSDYRDLVKQLNKTILAFAKVDNTVIIGRGGFVVLKDYQNV
                     LNVLLRAPLEHRIRNAMQTEQISDILEAEKIVRHSDEVRESFLQTFYNVSSNSAQYFS
                     LVIDTSRVPLHLAARWIMETAREIDRQKIPPERSSLNAEVDPVLVKAVREELEKSG"
     gene            complement(4966..5886)
                     /gene="prmA"
                     /locus_tag="PKL60_01905"
     CDS             complement(4966..5886)
                     /gene="prmA"
                     /locus_tag="PKL60_01905"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNW94920.1"
                     /translation="MDNKPKAWVEASVKCSGELAEAVAEVFARYCPEGVVLDSITTYD
                     AERHEHIPTGMMRVAAYLPQDDELETKKHSLEEALWYMGRIMPLSAPEFVSIEDQDWM
                     TLWKQNYQPLQVGQSWLILPAWLELPAGETRRVVRIDPAMAFGTGTHPSTQLCLLAIE
                     DSIRAGEPVIDLGCGSGILSIAALKAGASWALAVDIDEQAVSAAQANARINAVESKVE
                     ADQGSLADILAGKYSLRQAPLVLANILAPVLIRLFSEGLAGLVSPGGKLVLAGILENQ
                     AEDVLSAARAHGLALIAKYQQEDWVALVLG"
     gene            complement(5879..7015)
                     /gene="dnaJ"
                     /locus_tag="PKL60_01910"
     CDS             complement(5879..7015)
                     /gene="dnaJ"
                     /locus_tag="PKL60_01910"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNW94921.1"
                     /translation="MMATKRDYYEVLGVPKSASQDDIKNAFRSLARQYHPDVNKAHDA
                     EEKFKEINEAYGVLSDVEKRAAYDRYGPEGVNYQGMPDFSSMDLSDILEGLFGFGGFS
                     GMGSSGRRSRNTPRRGADLSSRVRLSFEEAVFGVEKEIEITRDEKCAVCGGSGVEPGK
                     KPVTCSTCKGQGEVRQVHQTFLGSMVQVGPCPVCGGRGEIIEDPCHSCRGSGLERRTV
                     KRKINIPAGVDNGNQVRVIGEGQPGSNGGPSGNFYLELEVQPHRYFRRKGNDLLLDLD
                     INMAQAALGDEISVPTLEGSEKLRIPPGTQPGKVFKLKDKGIPILNRQGSRGDQLVTV
                     NVQIPTQLSAEQKKLLETLGKTMGTEIKIQERSFLDKLKDVLGG"
     gene            complement(7098..8990)
                     /gene="dnaK"
                     /locus_tag="PKL60_01915"
     CDS             complement(7098..8990)
                     /gene="dnaK"
                     /locus_tag="PKL60_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNW94922.1"
                     /translation="MSKIIGIDLGTTNSVAAVMIGGEPVVIPSAEGERLVPSVVAVNK
                     NGERLVGRVARNQAIINPENTIFSIKRFMGRRYDDPEVQRAKSRVPYKITKAPNGDLR
                     VTLGDKEYTPPEVSAMILAKIKHDAEAYLGEPVTQAVITVPAYFNDAQRNATKDAGKI
                     AGLEVLRIINEPTASALAYGLDKRKNETIAVYDLGGGTFDISVLEVGDGLFQVKSTSG
                     DTFLGGDDFDQRLMDYLADEFKRENGIDLRQDRQALQRLKEAAEKAKIELSTTMQAEI
                     NLPYITADASGPKHLVTTLTRAKLEQLTEDLVDRSMKPVAQALSDANFRNGDINEVVL
                     VGGMTRMPAVQEAVRKQFGKDPHKGVNPDEVVAVGAAIQAGVLGGEVKDILLLDVTPL
                     TLSIETLGGIATPLIKRNTTIPTRESQVFSTASDSQTQVEIHVLQGERPMAADNKSLG
                     KFSLDGIPPAPRGVPQIEVIFDINANGILEVTAKDRATGRSQNITITASSGLSEAEVE
                     KMRREAEAHADEDQKRRDLIEARNTADNAVYSSEKTLRDLGEKVPADIKAKVEAAVEK
                     VKSVREHDDPAEINREVETLMQTLQQVGQAAYSQQPPQEENPQGGTGETPPDDGDVVD
                     GDFKQA"
     gene            complement(8999..9559)
                     /locus_tag="PKL60_01920"
     CDS             complement(8999..9559)
                     /locus_tag="PKL60_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNW94923.1"
                     /translation="MTDSKKTKKDPEEPLDNIAEQDHEQGPADGSELQALQSACESLK
                     AQSAEYLDSLQRERASFANYRKRVEQDNHLVYDNALADLIKAFLPVVDDLERALKNSP
                     SDPEADPWISGIALILQKLMKTLEAKGVSALDVKPGDEFDPAFHEAVTHEENPDYSDS
                     QVIEVVQTGYKLKDRVIRPALVRVAK"
     gene            complement(9603..10643)
                     /gene="hrcA"
                     /locus_tag="PKL60_01925"
     CDS             complement(9603..10643)
                     /gene="hrcA"
                     /locus_tag="PKL60_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNW94924.1"
                     /translation="MMKTELTERQKLLLSLIVHEHTRTAQPVGSKTLVEQFTLPISSA
                     TIRNEMTVLTEMGYLRQPHTSAGRVPTEEGYRFFVSNLAQQTTLPAPMRNTIAHQFYQ
                     ARQDLDGWLRLAASVLAAQSQAASIVTAPHTEKVAFKHLELISTRAAQVLMVLVLVGG
                     EIRQQILSLNDAATQEDLSTLASRINDLYANSSVEQITGMDPLADPTGAQVLEAVRQQ
                     MQLSQKLVTGEIYLDGVTNVLAEPEFADTEQARSALSLLEERSKLDDLLSRTILGNEI
                     GGVQVLIGGEGTWQELRQCSVVLTRYGIPDQLTGTVGVLGPMRMPYGRTISTVRFVAG
                     LLSDLVAENLIG"
     gene            complement(10764..11798)
                     /locus_tag="PKL60_01930"
     CDS             complement(10764..11798)
                     /locus_tag="PKL60_01930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW94925.1"
                     /translation="MEFSCVRTTAEWDALAPEWDLLFSRSHVQVPFLRFGYLRAWWQS
                     LGGAEWPPEKSALRIITARERGELVGIAPLFLSSKPEWPPALRLIGSVEVSDYLDLLS
                     PPERLADFLCGLLAFLRGDPELRGYPLELLNLREDSTTLQMLPAAAESSGYTYTEERL
                     QPSPYISLAESWEAYLAGVNKKQRHEIRRKVRNAENRHQTDWYSVQDASRLEEEVDDF
                     LAMMRQDPRKAAFLNPAMREHLQATARFAFGQGQLHLAFLTFEGHKAAAYFSFIEGGK
                     LWVYNSAWEPAYAPCSPGWVLLARVIEWAIAHGLREVDMMRGDEDYKYKFGAVNRYVT
                     KVCCLPPLPA"
     gene            11878..14292
                     /locus_tag="PKL60_01935"
     CDS             11878..14292
                     /locus_tag="PKL60_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94926.1"
                     /translation="MKHNLELISTNNQALPGFFGANIPRHAARGREEDSLLMLLSQQS
                     GEEVHPDLLKNWLENASAIFYKSSGSVTSAMRAVVFALNGALAERNLSLPPESIPISA
                     QLSLGVIHHDTLFIAQAGLSQALLLKTGSRALFYDADLDPRGLGLTQVPRIRFFQQAV
                     EEGDIILFSTQFPVGFLSGSSAEQPELSALLVELAASSPLTAEVGLARVRAGEGSARW
                     VDAAQGVESAEAPREEPAPLEPSAAASEPEVAPADEGVEAEESSRVERLPSEEVPAPA
                     EAENAQPLVEAPAPAPEKSEAPAELEPMLEEKALESEASDSYPPERISAQPTAEAPAV
                     STRRAARLYSEQTPEKTPRDRTAEKAARKQRSQNAYRKLADGAGSMDKLGEGLAAFLT
                     GEKKQSEGAPTESAELSRGTKLLLAILVPLVLVGLLSAVYFSQGRGNQYDYLMAQAQA
                     AADNAPAMQGAAAQRQAWNEVLSWLDQAETYRQSSEARALRVRAQDALDALDGALRLV
                     YKPAYASSLLTGLDISRIVSVGTDLYMLDKASGVVLHFSPSSSGFTPDMEFHCQPGLY
                     EGIRVGNLIDVLSVPINNAARAPILSMDANGNGLFCGVDKEPKAFTLIPPDGGFGRIG
                     AVLYDAGRLFILDPVKNALWIYRGASMNYTDAPDSYFEEKPVDLSEAVAAAVSGDELF
                     LLYSDGRSSHCLASNVTGTVECEDPYPYQDARGGAEAGTLDFDALHFSQLSYSPPPDP
                     SLYYLAAEQAELYQFSLRLNLNRVLRAGAGGGSLPGRPVTAFHVGSNRNVYLAFGSEL
                     YYAVIP"
     gene            14736..16280
                     /locus_tag="PKL60_01940"
     CDS             14736..16280
                     /locus_tag="PKL60_01940"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNW94927.1"
                     /translation="MSDVDPRLEQLHKILSEAQLGGGQARIDKQHAKGSLTARERILL
                     LLDEGSFRELEALVVQQNDPQAESTLGDGVVTGYGKVDGRPVYVYAQDFTVQGGALGE
                     MHSRKICRVMDLAAKNGAPIVGMIDSGGARIQEGVKSLGGYAEIFRRNAQYSGVIPQI
                     SLIMGPCAGGAAYSPALTDLIIMVQKQSYMFLTGPQVIKTVTGEDVDFESLGGADVHL
                     GISGTAHLVSPSERDALLLCRQVLAYLPANNVDSAPVLTSGDSPLRRAEALNTLVPIN
                     PNEPYSLHTAIQQIVDSGSFLEIQAGFAPNAVVGLARLDGRPVGIISQEPASMAGVMD
                     IDSADKISRMVRFCDAFNLPIVTFVDSPGFLPGVDQEHRGVIRHGAKVLFAYSEASVP
                     KVSVVTRKAYGGAYVVMSSKYLGTDITYAWPSAEIAVMGAEGAANILHRKQIAEAQDP
                     AAERARLEEEYRQKYLNPYAAARAGYIDEVIEPAQTRQKLIEALELLANKVESSAARK
                     HGNMPL"
     gene            16292..16627
                     /locus_tag="PKL60_01945"
     CDS             16292..16627
                     /locus_tag="PKL60_01945"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HNW94928.1"
                     /translation="MGETLLQGLSITGLGMGLVFLMIIALWGVMALLVRFTNHPEKAA
                     TSGEEAPAEQSDSEQTYAAQAAAAAVAYALVAEKHTTSAKTNAGQSAWLLSGRVRQNF
                     HTPGKGQKK"
     gene            16624..17043
                     /locus_tag="PKL60_01950"
     CDS             16624..17043
                     /locus_tag="PKL60_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNW94929.1"
                     /translation="MIINVSIAGKKYAVKIDDLHARPVKAEVDGEVFEIWPEEASPEL
                     SAPAAQAVLSKPMPAAAAPQAAPGGRDVNAPLPGVIIEINVKAGTRVKAGEPLLVLEA
                     MKMKNIIRAGRDGVIAEVLVSAGDQVRHNQPLFRFEA"
     gene            17056..18174
                     /locus_tag="PKL60_01955"
     CDS             17056..18174
                     /locus_tag="PKL60_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNW94930.1"
                     /translation="MDLTSLLTELGKGFANFSWQNAVMIAVGLVLIYLAIAKEYEPVL
                     LLPIGFGCLLANLGMSNEVGFMKVIYDAGIKTELFPLLIFVGVGAMIDFSPLLSQPSL
                     VLLGAAGQFGIYATLLLALLLGFPLNQAASIGVIGAIDGPTAIFVGSKLAPEIIAPIA
                     VAAYSYMSLIPIIQPPIMKLMTTRKERLIRMEYAPRPVSRLTLVVFPILVTLLVALVA
                     PDAAPLMAALMLGNLLREALVVDRLSKMAQNELINIATLFLGLAIGSTMTASSFLNLK
                     TLAILGLGLLAFVIDTIAGLLFGKLMALVTHNKVNPLIGACGISAFPMAGRLSAKMAL
                     EEDPSNFILMHAMGSNTAGQLGSVIAGGLLLALVMGVH"
     gene            complement(18813..19541)
                     /locus_tag="PKL60_01960"
     CDS             complement(18813..19541)
                     /locus_tag="PKL60_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW94931.1"
                     /translation="MVSKLSRIEEALLENSQVSVLCIEGNRNNNFAFAPDLARKGYEV
                     HAVSNGAEGIALLPEILPMVIVINAASLRTNGARIVRRFRALLPECPIVLIVKKGSPQ
                     DDVPEANIVLALPFTVQKLVNRIRTFQSIENRHVHELGPLLLNTRSNMVTCNGKEAHL
                     TPRLSKLLMHMMAEPNKVLPRKELFQKVWETDYTGDTRTLDVHISWLRQAVEEDPRCP
                     TLILTERSIGYRLHIPEHKPGQAD"
     gene            19820..20383
                     /locus_tag="PKL60_01965"
     CDS             19820..20383
                     /locus_tag="PKL60_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNW94932.1"
                     /translation="MKKMRECLISSVRVSLINQDRIVVLKEKDQERYLPIWIGLYEFE
                     AITIALHKIETARPLTHDLMKNLFGVLDARLLAVEITALESDTFYANLVIEQGGAQKR
                     VDCRPSDALALVVRTGVPVFVDEGVLDKAAVIPELEIHPEEGAAEASEEGTGDLSLFE
                     EFFNKLDDTDEDLPGPVNPDDGPNPVP"
     gene            20440..21807
                     /gene="dnaB"
                     /locus_tag="PKL60_01970"
     CDS             20440..21807
                     /gene="dnaB"
                     /locus_tag="PKL60_01970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNW94933.1"
                     /translation="MSDQDYNSGSAGDPGANQLFDRTMEEAVIGAVLVTPIKYLDVSS
                     ILQTDDFYYRSHQWIWQAFQRLYSSDPAFDLLTVKQELEAQGHLEEVGGVEYLASLTN
                     QVPSSLHAEAYARNISALAARRRLLTAANEVAKLAYRKDINVDTVIDQAEQSIFGVSQ
                     ERYKHDLVPIKDIAGQYYDRLTEMSLQTGAVSGLETGLKSVDKVLDGLQKSDFIIVAG
                     RPGQGKTGFLIGIAKHVGLQLKRNVAIFSLEMSSEQLLQRLLAQATDIDSQKLRSARL
                     SDEEMNRSVSALGVLAEANIYIDDTPAITPLQMRTKCRRLQMEYGLDLVIVDYLQLMS
                     AEVKTDNRVQEVSYISRYLKVLARELKVPVIAAAQLSRSVESRPDRRPVLSDLRESGS
                     LEQDADIVMFINQPNQEESSPFHTIARLEIAKHRNGPTDPGIELIFQKRLAMFRDQYY
                     GAVPD"
     gene            21818..22498
                     /locus_tag="PKL60_01975"
     CDS             21818..22498
                     /locus_tag="PKL60_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HNW94934.1"
                     /translation="MSASLPSGGFGSDPGTAWYLPADLLERAAETSLSADGMLAVLAY
                     FRLLGQPEEGSQAVRREALVEALSLPIFRQDAARTQLALEEALGAGFLIKYEDILLAG
                     TPRGKVLLGQLQAGAITAEQLNLVSQVPAPERPNIFRLYEANIGPLTPMMAEMLKADA
                     LEFPAEWIEDAVSEAVERNIRNWRYVRAILKTWKEKGRAEKAREPEGDLEKYRKLYED
                     QRRRKKHE"
     gene            22491..23837
                     /locus_tag="PKL60_01980"
     CDS             22491..23837
                     /locus_tag="PKL60_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW94935.1"
                     /translation="MSDQLQNITNADLPGDPDCPFCHGVGFVGKDVPFGHPEFGKLSV
                     CVCRARELAADRQKKLYELSNLGALQEKTFENFKPRGRVGLATQQANSLEQAYNSAAH
                     FAGTLQGWLLLLGRYGCGKTHLAAAIANKAVALGMETLFLTVPDLLDWLRFAYSDGAS
                     ESFEERFEKIRDVPLLVMDDFGTQNATAWAQEKLFQILNYRYINHLATVITSNNPLSS
                     FEGRIRSRLMDPELVTRVEILATDYRNPADDIGHPELSSLNLHARKTFESFSLRRDEA
                     IPSTDRATLKDALEAAQAFARDPQGWLVFIGPYGCGKTHLAAAIGNYHAQLGSPPLMV
                     VVPDLLDHLRAAFAPNSSVSLDQRFEEIRSARLLILDDLGTQSATPWAREKIYQLFNY
                     RYNAELPTVITTVNAPGEMDPRLQSRLSDARLCKVVIMSAPPYTGGGKGQGGRALR"
     gene            24338..26968
                     /gene="mgtA"
                     /locus_tag="PKL60_01985"
     CDS             24338..26968
                     /gene="mgtA"
                     /locus_tag="PKL60_01985"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF011702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNW94936.1"
                     /translation="MGTIDKRESQHPQGKNALPSGSESKAAQKLVSVAHLAVQDVLQQ
                     QKSQLEGLTESEVSARLEQYGYNEVVKEKQLGLVLRLLGMLKDPLTILLIVLAIISII
                     THDIRAAVVILTMVVLGVTLRFVQESRADSAAEKLKEMVSTMATVIRAGQAKSIPLRE
                     LVPGDIISLSAGDMVPADVRVLVAKDLFVNQSALTGESIPVEKWEVPADPGITNALEL
                     PNICFLGSNVESGTGSALVIHTGKDTYFGSLASSITGERQNTSFDQGINKFTWLMLRF
                     MMIMVPAVFLINGLSKHDWLQALLFAVAVAVGMTPEMLPMIVTTNLSQGALAMSKKKV
                     IIKRLNSIQNFGAMDVLCTDKTGTITQGKIVLEKHIDPRGNPSEKVLEFGYINSYFQT
                     GLKNLMDEAILQHDEHRDEIHRKWKKIDEIPFDFNRRRMSVIVEDENGLNMLVCKGAV
                     EEVMGLCSHVEADSQVLEVLPEHTEHRKKRVADLNADGFRVVAVAYKLMPGAPDEPVY
                     TVNDESDMVLAGFLAFLDPPKETAMEAIEKLHQLKVEVKILTGDNDVVTRRICGQVNL
                     PVEHIVLGSELDAMTPDQFSKTVEENAVFAKLSPAHKELIIAALRQNEHVVGFMGDGI
                     NDAPALKAADVGISVDSAVDIAKESSDIILLENNLLILQQGVIEGRKVFGNIMKYIKM
                     AASSNFGNMFSVVGASAFLPFLPMLPIQVLVNNLLYDFSQTTIPTDEVDQEWLVKPRK
                     WGIQGLERFILLIGPISSIFDYLTFALMLFVFHSKDNPALFHTGWFVESLFTQTLIIH
                     VIRTNKIPFIQSRASTPLMISSLLIVSIGAFLPFSPLASTLGFVPLPAAYWGYLAGML
                     VCYVALTQLAKTWFIRKFGE"
     gene            27284..29455
                     /gene="glgP"
                     /locus_tag="PKL60_01990"
     CDS             27284..29455
                     /gene="glgP"
                     /locus_tag="PKL60_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNW94937.1"
                     /translation="MNKIIREVPNHFNLPYRLKRLADLAHNLWWVWNPEAARLFKDID
                     PLGWDNCNHNPIVFLRTVNPVALENLTQDRYFLDRYDRIVRDFDYYMKTGKTWYAQNH
                     PELLEDEIAYFSFEYGLHESLMVYAGGLGVLSGDHLKEASDLGIPLVAVGFLYTYGYF
                     SQKITEDGWQEARHVQIIYEDLPLVALYDENHEPIKISIDLPGRKVFARLYELNIGRV
                     TLVLLQTNIPENNPQDRQLTDRLYISDPELRISQEILLGIGGLRALQALGHNASIYHM
                     NEGHSAFLTLERISQLMQSGKSYSEAADQVKRSSIFTTHTPVPAGNDEFPIWLIDKYF
                     SSYWPQLGLNRDEFMNIAFVHQEWGGEAFSMPVLALKLANYRNGVSRIHGDVSRHMWQ
                     QLWPDTPVDKVPISYITNGVHTGTWLARRYALLFNRYLGSDWKEHIDDKETWQRIDEI
                     PDEDIWRIRLHLKRKLVAYMVSRARTQWKTTTVHPVQTVASGVLLDPYALTIGFARRF
                     ATYKRANLLFRDVDRLLRIVSNERKPVQIVFAGKAHPADEPGKRLIQEVYQHVKDARF
                     GGRLVFLDDYDMDMARYLVQGVDVWLNTPRRPREASGTSGEKAALNGTLNFSVLDGWW
                     AEGYNGANGWAIGDDTEYADQDQQDNADAQSLYNTLENEIVPLYYTKRSADGLPGDWI
                     GRIKESIKSLAPQFSMTRMLKEYISDLYVPAISTAKEPPQE"
     gene            29478..30020
                     /locus_tag="PKL60_01995"
     CDS             29478..30020
                     /locus_tag="PKL60_01995"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HNW94938.1"
                     /translation="MDFLQNLSPIAGALMIFLLRLINYTMDTLRIMLTMRDKKLMSWI
                     LGFFESILFVVVMGAVLDDLDNILKVVAYAGGFATGNVVGMLIEKRLAIGYSHISIIS
                     RGLGKEISQVLRAHDFAVTEIPAYGRDGKVALLNCSVQRKLAAEVEKLALEQDPQAFI
                     TIEDVVPRQRGYWGRGGINR"
     gene            30042..30710
                     /locus_tag="PKL60_02000"
     CDS             30042..30710
                     /locus_tag="PKL60_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNW94939.1"
                     /translation="MQNLIGALIFDFDGLILDTESPEIQIWERLFTEAGGRFDHQAYL
                     GTIGSWDNSSYEPGVHLARLMDHGVTPQELVARVRMESAECIAQERPLPGVERLIARA
                     RGAGLRLALGSSSPAAWVQGHLRRLGLIQNFEILVTFDDVRQSKPSPEIFLTVLARLG
                     IGAEQALVLEDSYNGVLAANRAGIRAVAAPNAVTAAQDFSLAEEVLDTLDGLVLGKYF
                     PLAD"
     gene            31420..>31700
                     /locus_tag="PKL60_02005"
     CDS             31420..>31700
                     /locus_tag="PKL60_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94940.1"
                     /translation="MNVLVSGAAGFWAYHDTLELLASRRQLNVLVLASQAADAVVLAL
                     EQAQARARGPVCAENLIWREFLQRLGKILGKPKPILTLPKVLESLGAWF"
BASE COUNT         6533 a         9070 c         9366 g         6731 t
ORIGIN      
        1 gcgcgcacgg tcaggagacc ggcgcgatcc gagatatcct gcgctcggtc tggaggccgg
       61 cgcgatcaga gaagggcgcg ctcagaaatc gagcgcgccc tacatgcttt atggagtctg
      121 cgttggagga gcggcgactt ctaccaagga accattttca aatttgaatg tcctggagac
      181 tccaatcgtt ggaatcgttg acattggttc attcgcattc ctatctgcat aattcacgat
      241 gaactgagat ggattgtttg ggtcaatact tgtgctttgt ggcgcaattc tatctccgat
      301 gaatattgca ttcgttccca ggtacccatt ctctgttttg atggccgcca ctacgtaata
      361 gaaaatccca cttcccccat tttcttgttg caagaggaaa gccgaatcta ataaaccatc
      421 tgcgttgagg tcaatttcca cttgattgcc aaagaagcgg gttactagtt tggatgccga
      481 cccaggcgct aattctgttt ctgaaactcc attgaccagt gtaatcggtt gattgtcaat
      541 gaaataggtt ccatttttca ggctttcttc gggcgaagtt tcaacttcgg cagcttgtac
      601 tggtatttcg gaaggcactg ttgttggggc gttggttggt acaactgccg ttgggctagt
      661 gggcgcgcag gctgccaaaa caatgctggc catcacgatc aatacaattg agcatttccg
      721 tgttttcatg tcttttcctg taaatatttg attttgctta caatccggaa ttagacgttc
      781 aaactaatta aaatgttccc cgcccaactc tgatcgcgcc ggacttgcgc agcaccccgc
      841 agcggaggtg cttgtgggct gaccgcgcgc ggaggctgac cgccaggtct cccatgaaac
      901 gcctgcccga gcgcgctccg gtagaaagat gcatcgctag cgcccaggat gacaacaccc
      961 cccttcgttt tacgttacaa ttagcgcgat gattcatccc aaggagggtt catgagcaat
     1021 tccccattcc tatccaaacg cgccccggtg tacgctgaca tccctgatga gaaatggaac
     1081 gactggcact ggcagctcac ccaccggctg aacaccatcg aagacttcga ggcgctcttt
     1141 ccgcttactg aaagcgaaaa acaagcgctc tcttcccccg ggctgttccg cgtggaggtc
     1201 accccctact tcgcctcgct gattgacccc gaaaacccct ttgaccccat ccgccgccag
     1261 gtcatcccca ccgcccggga gctgcaaagc ttcaccggta tgatggagga ctcgctggcg
     1321 gaggacatgc acagccccgt gcccggcctg gtgcaccgct acccggaccg cgttttgatg
     1381 ctgattacca accagtgcgc cacttactgc cgctactgca cccgcgggcg cctggtgggg
     1441 gatccgcgcc agggctttat gcgcgatttt gaagcgcaga ttgcctacat ccgccgcacg
     1501 ccccaaatcc gcgacgtcct catctccggc ggggacccgc tgaccctctc gccggaacac
     1561 ctggaacgtg tgctgagctc cctgcgggaa atcccgcacg tggaaatcat ccgcattggc
     1621 tcccgcgtgc ccgtgttcct gccgcagcgc attaccccgc agctcaccgc gctgctggaa
     1681 aagtatcatc ccctctggct gaatatccac gtcaaccacc ccaacgagat tacggccgaa
     1741 ttggcggaag cctgcgaccg gctcagccgg gccggcatcc cgctcgggaa ccagtccgtg
     1801 ctgttggcgg gcgtaaacga cgacccgtac ctgatgcgcc ggctggtgca ggacctggcg
     1861 cgcatccgcg tgcgcccgta ttacatctac caatgcgacc tggtggcggg cgccgggcac
     1921 ttccgcacac ccgtctccaa agggctggag attctcgagg ctctgcgcgg gcatacctcc
     1981 ggcttttgcg tgcccaccta cgtggtggac gcaccgggcg gcggagggaa aatcccggtg
     2041 atgccgaatt atgtgctttc cagctcgccg gataaggtgg tgctgcgcaa cttcgaaggc
     2101 tacatcagcg cctactccga accgctggat taccagccgc ccgataagag cctgacagac
     2161 ccgtacaagc cgcgggaaga agacctgcag gaaggcgtgt tgggcttgct tcagggcagc
     2221 cagatgaata tcaaaccgga aggttttgac gcgctgcaca tgcgcggagg cgagctgcac
     2281 cgcctgcgca aagatgccca caagtggaaa gcgcttggca tcgggcacca ggaagcgggt
     2341 gaggagaatc cgcaggccgg gctttagcgc gtccgactgc ggcgcctgag ttccggcgtt
     2401 gcgccgtcag caaccagact gcccgataat ccggcatttc aggataatac cggaataaca
     2461 ggcagtggat gccctcagag gatattaccg cgggcatcaa gactggcgga atatcaggta
     2521 ttattgacaa atacccaaat atcgggtagt atggtgatgg aaccaggacc ctttgacagc
     2581 gatgaatccc tcccaaacaa atccgcacgc cttctgcatc aaagcggata tcatcggctc
     2641 ccgcggaagc cgccaggcgc agctgctgcc ggacgttgcg cgcgaactga acgcgcgctt
     2701 tgccagcggc ttgctgacgc cctttaccgt gcgggcgggg gacgagctgt tcggcgtttt
     2761 gataaacctg gagacgggct ttcaggccta taaggcgctt tattccctgt ccactgcgcg
     2821 cgggctgccc ttttacgtcg gcatgggcgc gggcagcgtg gacctgacga gcagcgcgga
     2881 cgcggagctg gtgaacgggg aagccatctg gctggcggcg gaagcgctgg agcagctgaa
     2941 gggcaagccg gataccgaac tgcttgcccg ccaaaagcgc gccaggttcc gctacgccat
     3001 cgccgtaagc gaagaccccg ccgaaaacca gccgctgcag catttcctgt tctacatcat
     3061 gagccgtgtc agccggcgca ctgcgctgca gcagcaggct gccgcgctga aggaagcgca
     3121 tccggattgg gataacctgc gcttataccg cgcgctggag ggcaaagagc aggaagtcat
     3181 cgaagcggaa aacgccatcg caaacttcag caaactgctg cggcgcggcg actaccagtt
     3241 agtgcgggac gcggaagagg cgtttctgtt cctcatccgg cgcttttgct gcggcgggtg
     3301 agcgactgcc tgccttgccg gattagtgtt cgtttgttcg tggagcgaag atgataaaac
     3361 ccctggccat cctcattctg gcgcatttgc tggcggattt cattctgcag ccggcttccc
     3421 tgattgccct caagcagaac aaaaaccgcc cccctaagtt ctggaacagc ggcatcttct
     3481 ggcacagcgt ggtgcacctg gcggtcagca cggcgctgct ggtgctgtgg ggcttgtggg
     3541 gctggcaggc ggggctggct gtgttgtgca ttttcaccgc gcattacctg attgaccgct
     3601 tcaaattcct gtggaagttc cagggcacct gggtctttat cctggaccag gcgctgcact
     3661 ttttggcgat ttatctcatc ctgctggcat tcgggctggc ggccgggccg ataccgctgg
     3721 tggggttggc ggtatctttt ggtcagcaca gcctgcccct cagcctgtcg aacaagctgg
     3781 ccctgggggc gatatgcgtc atcgggctgg tgtgggtgag cgggcacctg gtcaagaata
     3841 tccttggttc gcttcggctg cgttcatttg agagcctgaa gaaagccgaa ttggaaaaca
     3901 gcgccgcgcc ggagcctgga aattccccga cgcaaacaga aatcagggac gcgcagccgg
     3961 ctgaagccag cacgggcctg tatatcggct acctggaacg gctgctggtg ggcgtcttta
     4021 tggtatcggg gatttatacc gggctgggtc tgctgggcac gctcaagacg ctcgcgcgct
     4081 tcaagcaatt agagaaccgc gaagtaacag aatatttcat tctgggaact atgttgagca
     4141 tgctgctggg aatgctcttc ggcgcgctcc tgcgcttcat tattctgtac tgaacggacg
     4201 ggcatcaacc gcttttttcc agctcttccc gcacggcttt taccagcacc gggtccactt
     4261 ccgcgttgag cgagctgcgc tccgggggaa ttttttgcct gtcaatttcc cgcgcggttt
     4321 ccataatcca gcgggcagcc aggtgcagcg gtacgcggct ggtgtcaatc accagcgaga
     4381 aatactgcgc ggagtttgaa ctgacgttat aaaaagtctg taaaaagctt tcgcgtactt
     4441 catcgctgtg ccgcacaatc ttttccgcct cgaggatatc tgaaatttgt tcggtttgca
     4501 tcgcgttgcg gatgcggtgc tccagcgggg cacgcagaag cacgttgagc acgttctggt
     4561 aatccttaag caccacaaaa ccgccgcgcc cgatgataac cgtgttgtcg acttttgcga
     4621 aggccagaat tgtcttgttc agctgcttca ccaggtcccg gtagtcgctg tcgaacagcg
     4681 accagacatt atgagtggaa tcgtagacct tattaaatga aagcatcccg aattgcgcca
     4741 ggacttttcc gattatttct ttgttcacca gatgatagtt caaggaattt atgaccatct
     4801 ggctaatcag gccgccgcca gtgccgagct cacgtgacat cgtgattatt gccatgcgcg
     4861 cctcctttct gcttaactga agtataaaga cattgggggc tttaagcaac ctgttcccgc
     4921 cagattagcg ggaacagagg aagaaacacg caaaatcagg cggtttcagc cgaggaccaa
     4981 agcgacccaa tcttcctgct ggtatttcgc gatgagcgcg agcccatgcg cgcgggctgc
     5041 cgagagcacg tcttcggcct ggttctccag aatgccagcc agcaccagct tgccccccgg
     5101 cgataccaac ccggcaagcc cctcgctgaa caagcggatc agcaccggcg cgagaatgtt
     5161 ggccagcacc agcggcgcct gcctgagcga gtacttgccg gccagaatat cggccagcga
     5221 cccctggtct gcttccactt tgctctcgac cgcgttgatg cgggcgttgg cctgcgcggc
     5281 gctgaccgcc tgctcgtcga tatccaccgc cagagcccac gacgcgcccg ctttgagcgc
     5341 ggcaatgctg aggatgccgg agccgcagcc caggtcaatc accggctcgc ccgcgcggat
     5401 gctgtcctcg atggcgagca ggcacaactg cgtggagggg tgcgtgccgg tgccgaaggc
     5461 catggcgggg tcgatgcgca ccacacggcg cgtttccccg gcgggcagct ccagccaggc
     5521 aggcaggatg agccagcttt ggccgacctg cagaggctgg tagttttgct tccagagcgt
     5581 catccaatcc tggtcttcga tgctgacaaa ttccggcgcg gacaggggca taatgcggcc
     5641 catgtaccag agcgcttctt ccaggctgtg ctttttagtt tccagttcgt catcctgcgg
     5701 caggtaggcg gccacgcgca tcatgccggt ggggatgtgc tcgtgccttt ccgcgtcgta
     5761 ggtggtgata ctgtccagga ccacgccctc cgggcagtag cgcgcgaaga cctccgcgac
     5821 ggcttcggcc agctctccgc tgcatttcac cgaagcttcc acccaggctt tcggtttatt
     5881 atccaccaag cacgtccttc aatttgtcca aaaaactgcg ttcctggatt ttgatttcgg
     5941 ttcccatggt cttcccgaga gtttccagca gcttcttctg ttcggcgctc aactgggtcg
     6001 ggatttgcac attgacggtt accaactggt cgccgcggct gccctggcgg ttaaggattg
     6061 ggatgccttt gtccttcaat ttaaacactt tgcccggttg ggtgcccggc gggatgcgca
     6121 gtttctcgct gccttccagg gtcgggacgc tgatttcatc gcccaaggcg gcttgcgcca
     6181 tgttgatatc caggtccagc agcaaatcgt tgcctttgcg ccggaagtag cggtgcggct
     6241 ggacttccag ctcgagataa aaattgccgc tgggtccgcc gttactgccg ggctgacctt
     6301 cgccgatgac gcgcacctgg ttgccgttgt ccacgccggc ggggatgttg attttccgtt
     6361 tgacggtgcg acgctccagc ccactgccgc ggcaggaatg gcaggggtct tcgatgattt
     6421 caccgcgtcc gccgcatacc gggcagggac cgacctgcac catggagccc aaaaaggttt
     6481 ggtgcacctg gcggacttca ccctggcctt tgcaggtgct gcaggtgacg ggcttcttgc
     6541 cgggttccac accggagccg ccgcaaacgg cgcatttttc atcgcgcgtg atttcgattt
     6601 ctttttccac gccgaacacg gcctcttcaa agctcaggcg cacgcgcgag ctcaggtcgg
     6661 cgccgcgccg tggggtattg cgcgaacggc ggccggagct gcccatgcct gaaaatccgc
     6721 cgaagccaaa cagcccttcc aggatatccg aaaggtccat gctgctgaaa tccggcatgc
     6781 cctggtagtt cacgccttcc ggaccgtagc gatcgtaagc ggcgcgtttt tccacgtcgc
     6841 tcaagacgcc gtaggcttcg ttgatttcct tgaatttctc ttcggcgtcg tgcgccttgt
     6901 tgacgtccgg atggtactgc cttgccaggg aacggaacgc gttcttaata tcgtcctggc
     6961 tggcagattt cgggacgcca agcacctcgt aataatctcg ttttgtcgcc atcatgttca
     7021 gaacaccatg ctatcaacaa ccaacacccc gaaggctttt gcacgtcccg cgggtgttgg
     7081 ctgcaatttc tcgaggctta agcctgttta aagtcgccgt cgaccacgtc gccgtcatcg
     7141 ggcggggtct ctcccgtgcc gccctgcggg ttttcctcct gcgggggctg ttgggaataa
     7201 gcggcttgac cgacctgctg cagggtctgc atcagggttt ccacttcgcg gttgatttcc
     7261 gcggggtcgt cgtgctcgcg cacggatttg accttctcca cagcggcttc cacttttgcc
     7321 ttgatatcgg cgggcacttt ttcgcccagg tcccgcagcg tcttctcgga gctgtagacc
     7381 gcgttatcag cggtattgcg cgcttcaatc aggtcgcgcc gtttttggtc ttcgtcagcg
     7441 tgggcttcgg cttcgcggcg cattttttcc acttccgctt cgctcaagcc ggaggaggcg
     7501 gtgatggtaa tgttttggct gcgcccggtg gcgcggtctt tggcggtcac ttccagaatg
     7561 ccgttggcgt tgatgtcaaa aatgacctca atctgcggca cgccgcgcgg agcgggcgga
     7621 atgccgtcca gcgagaactt gccgagcgac ttgttgtccg ccgccatggg gcgttcgcct
     7681 tgcagtacgt gaatttccac ctgggtctgg ctgtcggacg cggtggagaa aacctgtgat
     7741 tcgcgggtcg ggatggtggt gttgcgctta atcagcgggg tggcaatgcc gcccagcgtc
     7801 tcgatggaca gggtgagagg ggtcacgtcg agcaggagga tatccttgac ctcgccgccc
     7861 aacacgccgg cctggatggc cgcgcccacg gctacaacct catccgggtt gacgcctttg
     7921 tgcgggtcct tgccgaactg cttgcgcacc gcttcctgca cggcgggcat gcgagtcatg
     7981 ccgcccacca gcacaacttc gttgatatcc ccattccgga aattcgcgtc ggagagcgcc
     8041 tgcgccacgg gcttcatgga gcgatccacc aggtcttcgg tcagctgctc cagcttggcg
     8101 cgggtgagcg tggtaaccag gtgcttgggc ccggaggcgt cggcggtgat atagggcagg
     8161 ttgatttccg cctgcatggt ggtggaaagt tcgattttgg ctttttcagc ggcttcctta
     8221 aggcgctgca gagcctggcg gtcctggcgc agatcgatgc cgttctcgcg cttgaattca
     8281 tccgcgagat agtccatcaa acgctggtca aagtcatcac cgcccaggaa ggtatccccg
     8341 ctggtggatt tcacctggaa gagcccgtcg ccgacctcca gcacggagat gtcgaaggtg
     8401 ccgccgccca ggtcgtacac ggcgatggtt tcgttcttgc gtttatccag cccgtaagcc
     8461 agagcggacg cggtgggttc gttaatgatg cgcagcactt ccagccccgc gatttttccg
     8521 gcgtctttgg tggcgttgcg ctgggcgtcg ttgaagtacg cgggtacggt gattaccgcc
     8581 tgcgtaactg gctcgcccag gtaggcttcg gcatcgtgtt tgattttcgc cagaatcatc
     8641 gcggaaacct caggtggggt gtactcttta tcgcccaacg tcacgcgcaa gtctccgttg
     8701 ggagccttgg taatcttgta aggcacacgc gatttggcgc gctgcacttc cgggtcgtca
     8761 tatctgcgcc ccataaagcg cttgatggag aaaatggtat tctccgggtt aataatggcc
     8821 tggttgcgcg ccacgcgccc caccagccgc tcgccgtttt tgttcaccgc tacgacggaa
     8881 gggaccagcc gttcgccttc cgcggaaggg atgacgacgg gctcgccgcc gatcatcacc
     8941 gccgcgacgg aattggtagt gcctaaatcg ataccaataa tctttgacat gaatgctcct
     9001 attttgctac ccgtaccagg gcgggtctga ttacacgatc tttaagtttg tagccggtct
     9061 gaacgacctc gatgacctga ctatcggaat agtccgggtt ttcctcatgg gtgacggcct
     9121 catgaaaagc cggatcgaat tcgtctccgg gctttacatc gagcgcgctc acgcctttgg
     9181 cctccagcgt cttcatcagc ttttgcagga tgagcgcgat gccgctaatc caggggtcgg
     9241 cttccgggtc ggaggggctg ttcttgagcg cccgttccag gtcgtccacc accggcagga
     9301 aagccttgat gagatccgcc agggcattgt cgtagaccag gtggttatcc tgttccacgc
     9361 gcttgcggta gttggcgaag ctcgcccgtt cgcgctgtag actgtccaga tattcagcgg
     9421 attgagcttt caggctctca caggcgcttt gcagggcttg cagttcgctg ccgtctgccg
     9481 ggccttgttc atgatcctgt tcggcgatgt tatccaaagg ttcttcagga tcttttttcg
     9541 tttttttaga atctgtcatc tctttcctcg ttcgtattcg cgcgtcaggc gcgcggtatg
     9601 atctatccaa tcaggttttc ggctaccagg tctgagagca aaccggccac gaagcgcacg
     9661 gtggagatgg tgcgcccgta gggcatgcgc atgggaccca ggacgcccac tgtgccggta
     9721 agctggtccg ggatgccgta gcgggtcagg actaccgaac actggcgcag ttcctgccag
     9781 gtgccttccc cgccgatgag cacctgcacg ccgccaatct cgttgcccag gatggtgcgg
     9841 gaaagcaggt catccagctt gctgcgttct tccagcaggg agagggcgga gcgggcctgc
     9901 tcggtatcgg cgaattcggg ctcggccaac acgttggtca cgccgtccag gtaaatctcg
     9961 ccggttacca gcttctgcga gagctgcatt tgctgccgta cggcttccaa cacctgcgcg
    10021 ccggtcgggt cggccagcgg gtccatgccc gtgatttgct ccacggagct gttagcgtac
    10081 aggtcgttga ttcggcttgc cagcgtggaa agatcctctt gcgtggcggc gtcgttcagg
    10141 gagaggattt gctgccggat ttccccgcca accaatacca gcaccatcag cacctgggca
    10201 gcgcgcgtgg aaatcagttc cagatgcttg aaagccacct tttcggtatg cggcgcggtg
    10261 acaattgaag ccgcctgaga ctgcgcggca agcaccgaag ctgccaggcg cagccagccg
    10321 tccaggtcct ggcgggcctg gtaaaactgg tgcgcgatgg tgttgcgcat aggggccggc
    10381 aaagtggtct gctgcgccag gttgctgacg aaaaagcggt atccttcttc cgtcgggacg
    10441 cgcccggcgg aggtgtgcgg ctggcgcagg tagcccattt cggtcaacac ggtcatctcg
    10501 ttgcgaatcg tggcggaact gatgggcagc gtgaactgct cgaccagcgt ttttgagccg
    10561 acaggctggg cggtgcgcgt gtgttcgtgc acaatcagcg agagcagcag tttctggcgt
    10621 tccgtaagtt ctgttttcat catagtctga ttctattagc actctcaggc tgagagtgct
    10681 aaattaacat tcctaatcat accacagcgc ggctaaaatt caagcgctga gtgctaagca
    10741 gtccgtaaga tttctgtaag aacttatgcg ggcagaggcg gcaggcagca caccttggtg
    10801 acgtagcggt tgaccgcccc gaatttgtac ttgtaatctt catcgccgcg catcatgtcc
    10861 acctcacgca gcccatgcgc aatcgcccac tcgataaccc tggccagcag cacccaaccc
    10921 ggcgagcagg gcgcgtaggc cggctcccag gcggaattat agacccacag cttgccgcct
    10981 tcgataaagc tgaaataggc agccgctttg tgcccctcga aggtcagaaa tgccaggtgc
    11041 agctgcccct gcccaaaggc gaagcgggcg gtggcttgca gatgctcgcg catggcaggg
    11101 ttgagaaagg ctgctttgcg cgggtcctgc cgcatcattg ccaaaaagtc gtccacctct
    11161 tcttccaggc gggaggcatc ttgcacgctg taccagtcgg tttggtggcg gttttctgcg
    11221 ttgcggactt tccggcggat ttcgtggcgc tgcttcttgt tcacacccgc caggtaagcc
    11281 tcccagctct ccgccaggct gatatagggg gagggctgca agcgctcttc ggtataggta
    11341 tagccgctgc tttccgcggc ggccggcaac atctgcagcg tagtggaatc ttcgcgcaga
    11401 ttgagcagtt caagcgggta cccgcgcagt tccgggtctc cccgcaggaa ggcgagcagg
    11461 ccgcaaagga agtccgccag gcgttccggc gggctgagca ggtccagata gtccgagact
    11521 tcaacggagc caatcaggcg cagggcgggc ggccattcgg gcttggagct cagaaagagc
    11581 ggggcaatgc cgaccagttc gccgcgctcg cgggcggtga tgatgcgcaa ggcgcttttt
    11641 tccggtggcc attccgcgcc gcccaggctt tgccaccagg cgcgcagata cccgaaacgt
    11701 aaaaaaggga cctgcacatg acttcgcgag aagagcaaat cccattccgg agcaagcgca
    11761 tcccattcgg cggtggtgcg cacacacgaa aattccataa cctgattata cctttatggg
    11821 ccagcgtgta atgtataatg tacttggttt ggagtatttt agacgctggg acggttcatg
    11881 aaacataatc ttgagcttat cagtacgaat aaccaggcgc tgccgggctt ttttggcgcg
    11941 aacattcccc ggcacgcggc gcgcgggcgc gaagaagaca gcctgcttat gcttttatcc
    12001 cagcaaagtg gggaggaagt tcatcccgac ctgcttaaaa attggctgga gaatgccagc
    12061 gcgatttttt acaaaagctc cggttcggtc acgtccgcca tgcgcgcggt ggtctttgcg
    12121 ctgaatggag ctctggcaga gcggaatctc agtctgccgc cggaaagcat tcccatttcc
    12181 gcccagcttt ccctgggcgt catccatcat gatacgctct tcatcgcgca agccggactc
    12241 agtcaggcgc tcctgctcaa aacgggcagc cgggctttat tctatgatgc cgacctggac
    12301 ccgcgcgggc tggggctcac gcaggttccg cgcatccgct tttttcaaca ggcggtcgaa
    12361 gagggcgata tcatcctgtt ttccacgcag ttcccggtgg gcttcctgag cggcagcagc
    12421 gctgagcagc ctgaactgag cgcgctcctg gtcgaattag ccgccagcag ccctctgaca
    12481 gccgaagttg gtctggcgcg ggtgcgggcg ggggaaggct cggcgcgctg ggtggacgcg
    12541 gctcagggcg tggaaagcgc ggaagctccg cgggaggagc ccgccccgct agaaccatcc
    12601 gcggccgcaa gcgaaccaga ggttgctccg gcggatgagg gggtggaggc agaggaaagc
    12661 tcccgggtgg agcggctgcc ttcggaggaa gtcccagcgc cagcggaagc agaaaacgcg
    12721 cagccgcttg tggaagctcc ggccccggcg cccgagaaga gcgaagcgcc ggccgaactg
    12781 gagcccatgc ttgaagagaa agcgctcgag agcgaagcca gcgattccta cccaccagaa
    12841 cggattagcg cgcagcccac agcggaagcc ccagccgtat ccacgcgtcg ggcggcacgc
    12901 ctttacagcg agcaaacccc ggagaaaact ccgcgcgacc gtaccgcgga aaaagccgcg
    12961 cgcaaacagc gcagccaaaa cgcgtaccgc aagctcgcgg acggagccgg ctcgatggac
    13021 aaattaggcg aggggctggc tgcgtttttg accggcgaaa agaaacagtc cgagggagcc
    13081 ccgaccgaaa gcgccgaact ctcacgcggg acaaagctgc tgctcgcgat tctggtgccg
    13141 ctcgtgttgg tggggctgct ttccgcggtt tatttcagcc agggcagggg caaccaatat
    13201 gattacctga tggcgcaagc ccaagccgcg gcggataacg ccccggccat gcaaggcgct
    13261 gccgcgcaaa ggcaggcctg gaacgaagtg ctttcctggc tggaccaggc ggaaacttac
    13321 cgccagagca gcgaagcgcg ggcactgcgc gtgcgcgcgc aggacgccct ggacgcgctg
    13381 gacggcgcgc tgcgcctggt ttacaagccc gcgtacgcct caagcctgct caccgggctg
    13441 gatatcagcc ggattgtgtc ggtcgggact gacctttaca tgttggataa ggccagcggc
    13501 gtcgtgctgc atttcagccc ttccagcagc ggtttcacgc cggacatgga gttccattgc
    13561 cagccggggc tgtatgaagg catccgcgtg ggcaacttaa tcgatgtgct ttccgtgcca
    13621 atcaataacg ccgcccgcgc gccgatattg tccatggacg cgaacggtaa cggcctgttt
    13681 tgcggggtgg ataaggaacc caaggccttc accctgatcc ccccggacgg gggttttggc
    13741 aggattgggg ccgtgctata tgatgccggc cgcttgttca tcctggaccc ggtcaagaac
    13801 gcgctctgga tttatcgcgg cgcgagcatg aattacaccg acgcgccgga ttcttatttc
    13861 gaggagaaac cagtggacct gtcggaggct gttgcggcag cggtcagcgg ggatgaactt
    13921 ttcctgctgt actcggacgg gcgcagttcg cattgcctgg cttcgaacgt caccggcacg
    13981 gtggagtgcg aagacccgta cccgtatcag gatgcgcgcg gcggcgcgga agccgggacg
    14041 ctggactttg acgcactgca tttcagtcag ctgagttact cgccgccccc ggacccttcg
    14101 ctgtattatc tggcggcaga acaggctgag ctgtaccagt tcagtttgag attgaacctg
    14161 aaccgcgtgc tgcgggcggg ggcaggcggc ggcagtttac ccggccgacc ggtcacggcg
    14221 tttcatgtgg gttccaaccg caatgtgtac ctggcatttg ggagcgagct gtattacgcg
    14281 gtgattccct gagcttttga gggcgcgggt ttcttgagga gaattggctg acggatctca
    14341 agccgccgat aaaggtgcct gaagggcggg tgagggtctg ttttcgattg gtgttgccgg
    14401 gttaatgcgc gcagcaccgt ccacgaaaag caacgctggg acgcaggcgg aaggcgaagc
    14461 atcctggata ttttgttccc aagagaaccc cccatgtttt tgggatgctc tgcgcggaca
    14521 tctgcttacg gtatgtatgc ccgcgcgcgg gttgtgctgc tggaaaggac cctcagcagt
    14581 cgcgctgcgc tcgccagatt cccgaagccc gattggcgcg ccccaatttc cccccaatac
    14641 cgccagtgtt gatgttacaa ttacccaact cggctgcgga caaaacctca ccctaaccgg
    14701 cagccatgct aactttgttc ttatgaagga gcaatatgtc tgatgtagat ccccgcttgg
    14761 aacaactcca caagatactt tctgaagctc aattgggcgg cggtcaggcc cgcattgaca
    14821 aacagcacgc caagggcagc ctcaccgccc gcgaacgcat tctgctgctg cttgacgaag
    14881 gcagtttcag ggagttggaa gctttggtgg tgcagcaaaa cgacccgcaa gccgaatcca
    14941 cgctgggaga cggcgtggtg actggctacg gcaaggtgga cgggcgtccg gtgtacgttt
    15001 acgcccagga tttcaccgtg cagggcggcg ccttgggcga gatgcacagc cgcaaaatct
    15061 gccgcgtgat ggatctggca gccaaaaacg gcgcgccaat cgtcgggatg atcgattccg
    15121 gcggcgcgcg catccaggaa ggcgtgaaaa gcctgggcgg ctacgcggaa attttccggc
    15181 gcaacgcgca atactccggg gttattccgc agatcagcct gattatgggc ccttgcgctg
    15241 gcggcgcggc gtattccccc gcgctgaccg acctgattat catggttcag aagcagtcat
    15301 acatgttcct cacgggtcca caagtgatta agaccgtgac cggagaagac gtggactttg
    15361 aatccctggg cggcgcggac gtgcacctgg gcattagcgg cacggcgcac ctggtgagcc
    15421 ccagcgagcg cgacgcgctt ctgctctgcc ggcaggtttt ggcttacctg cccgccaaca
    15481 acgtcgattc cgcgccggta ctgaccagcg gggacagccc cttacggcgc gcggaagctt
    15541 tgaacacgct cgtgccgatc aacccgaacg aaccctacag tctgcatact gctattcaac
    15601 aaatcgtgga cagcggctct ttcctcgaaa ttcaagccgg cttcgctccc aacgctgtcg
    15661 ttggcctggc gcgcctggac ggcagaccgg tgggcattat ttcccaggaa cctgccagca
    15721 tggcgggcgt gatggatatt gactcggccg acaaaatcag ccggatggtg cgcttctgcg
    15781 atgccttcaa tctgcctatc gtaaccttcg tggactcgcc cggttttctg ccgggggtgg
    15841 accaggagca ccgcggcgtc atccgccacg gcgccaaagt gctgttcgcc tactccgaag
    15901 ccagcgtccc caaggtcagc gtggtcacgc gcaaagctta cggaggcgcg tatgtggtca
    15961 tgagcagcaa ataccttggc acggatatca cctacgcctg gcccagcgcg gaaattgccg
    16021 tgatgggcgc ggaaggcgcg gcgaacatcc tgcaccgcaa gcaaattgct gaggcgcaag
    16081 acccggcggc cgagcgcgcg aggttggagg aggaataccg ccagaaatac ctgaatcctt
    16141 acgcggctgc ccgcgccggg tacatcgatg aagtcatcga gcccgcgcag acccgccaga
    16201 aactgatcga agcgttggaa ctgctggcga acaaagtgga aagttccgca gcgcgcaagc
    16261 acggcaatat gccgctgtag gcaggtgcgc tatgggagaa acattgctgc aaggtttatc
    16321 catcaccggc ctgggaatgg ggctggtctt tttgatgatc atcgcgctgt ggggtgtcat
    16381 ggcgctgctg gtgcgcttta cgaaccaccc ggaaaaggca gccacaagcg gtgaagaagc
    16441 cccggcggag caaagcgata gcgaacagac ttatgccgcg caggcagccg ctgccgcggt
    16501 ggcgtacgct ctggtggcag aaaaacacac aacgagcgcc aaaaccaacg ccgggcaaag
    16561 cgcgtggctg ttaagcggac gggtgcggca gaactttcat actccaggca aaggacagaa
    16621 aaaatgatta ttaacgtcag catcgcgggt aaaaaatacg cagtaaaaat tgacgacctg
    16681 cacgcgcgcc cggtaaaagc cgaagtggac ggcgaggtct ttgaaatctg gccggaagaa
    16741 gcctccccgg agctaagcgc gcctgccgcg caggctgttc tcagcaagcc tatgcccgcc
    16801 gcggccgcgc cgcaggccgc acccggcgga cgggacgtaa acgccccgct gcccggcgta
    16861 atcattgaaa tcaacgtgaa agccggcacg cgcgtgaaag ccggcgagcc tttgttggtt
    16921 ttggaagcga tgaaaatgaa gaatatcatc cgcgccggca gggatggcgt tatcgcggaa
    16981 gtgcttgttt ccgctggcga ccaggtgcgg cacaaccagc cgctcttccg ctttgaagcc
    17041 taaaaggaca cttttatgga cctgacaagc ctgttgaccg aattaggaaa aggtttcgcg
    17101 aactttagct ggcagaacgc ggtgatgatt gccgtggggc tggtgcttat ctacctggca
    17161 atcgccaaag aatacgagcc ggtactgctc cttccgattg gctttggctg tttgcttgcc
    17221 aacctgggca tgtccaacga agtcggcttt atgaaggtga tttacgacgc cggcatcaag
    17281 accgaactgt tcccgctgct tatttttgta ggcgtaggcg cgatgattga cttcagtccg
    17341 ctgctctcgc agcccagcct ggtgctgctg ggggcggccg ggcagtttgg catttacgcc
    17401 acgctgctgc tggcgctcct gttgggcttt ccgctcaatc aggcggcctc catcggcgtt
    17461 atcggcgcga tcgatgggcc aaccgcgatt tttgtgggca gcaagttggc gcctgaaatc
    17521 atcgcaccga ttgcggtggc ggcgtactcg tatatgagcc tgattccaat tatccagccg
    17581 ccgattatga agttgatgac cacccgcaag gaacgtctga tccgcatgga atacgcgcca
    17641 aggcctgtct cgcgcctgac gctggtagtt ttcccgattc tggtgacgct gttggtagcg
    17701 ctggtggccc ccgacgccgc gccgctgatg gccgcgttga tgctgggcaa cctgctgcgc
    17761 gaagcgcttg tggtggaccg cctgagcaag atggcgcaaa atgagctgat taacattgcc
    17821 acgctcttcc tgggcctggc gattggttca acgatgaccg cttcgagttt tctgaacctg
    17881 aaaaccctgg cgattctcgg gctggggctg cttgcttttg tgattgatac gatagccggg
    17941 ctgctcttcg gcaagctgat ggcgctggtt acccacaaca aggtcaatcc gctgattggc
    18001 gcttgcggca tcagcgcttt cccgatggcg ggcaggctct ccgcgaagat ggcgttggag
    18061 gaagacccct caaatttcat cctcatgcac gctatggggt ccaacacagc gggtcagctc
    18121 gggagcgtga ttgccggcgg gctgctgttg gcgctggtga tgggcgtgca ttgagtcctt
    18181 tttcgattat gacagccgcc ttcacaggcg gctgtttttt aatatgcttt ccgcgcgagg
    18241 agaacatcgt ttcgctgagc tcgcgatgac gaaatagccg tcattgcgaa ggaacgttgg
    18301 tgactgaagc aatctccccc tatgccagcc gggagatcgc ttcgcaggct cgcgatgacg
    18361 atttgtgtca tgctgagccg caggcgaagc atctcgtcag ttctaagcgg aatccttcgc
    18421 tgacgacctg tgcccgcaag ggtatcagga tgacaacagg gaattaataa gagccatcag
    18481 cgaagcattt tgttgggaaa aacgcgatcc ttcgctggcg ctctggatga caaggataat
    18541 tcaggatgac aagaataaat caggacgaca agtttggttc aaagtgccaa tagtatgtca
    18601 tgctgtgcca catgcgaagc atcttgtcag tcctacgcgg gatccttcgc caaagctcag
    18661 gatgactaaa atatttctgg atgacaagtt tttttgggta gcaagagaag cttaacactc
    18721 gccaacaaag cttcatccgg tcaattaacc gccttgccgc gtaaataaaa aacgcggctg
    18781 gcgaccgcgt gcagcacatc ccctggcagg ctttaatccg cctggcccgg tttgtgctca
    18841 gggatatgca gcctgtaacc gattgagcgt tccgtcagga ttaacgtggg gcagcgtggg
    18901 tcctcctcca cagcctgccg cagccagctg atgtgaacgt ccagcgtgcg ggtatcaccg
    18961 gtgtagtcgg tttcccagac tttctggaaa agctctttgc gtgggagcac cttgtttggc
    19021 tcggccatca tatgcataag taattttgaa aggcgcgggg tgagatgggc ttctttcccg
    19081 ttgcaggtga ccatattgga gcgcgtgttg agcagcaagg ggcccagctc gtggacgtgg
    19141 cggttttcga ttgattggaa agtccggatg cggttgacca gtttctgcac ggtaaagggc
    19201 agcgccagta cgatattcgc ttcaggcaca tcgtcctgcg gtgacccttt tttgacaatc
    19261 agcacaatcg ggcattccgg cagcagcgcc cggaagcgcc ggacgatgcg ggcgccgttc
    19321 gtgcgcagcg aagccgcgtt gatgacgatc accatcggga gaatttcggg cagtagagcg
    19381 atgccttcag cgccgttgct gaccgcgtgg acttcgtaac ctttgcgtgc caggtccgga
    19441 gcaaacgcaa aattattatt gcgattgccc tcaatgcaaa gtactgaaac ctgggaattc
    19501 tccagcaaag cttcctcaat ccttgataat ttgctgacca ttcggctgca gcttgcttcc
    19561 tagggtgaag ggtaatgttt ttacagaccc aatcccgaaa aacaacaccc ccgaagcctg
    19621 caatcgccgg tggttaaatg acataccgat cgtttattgt aaaaatatta tagccgaatc
    19681 ttagcgcaag tggggcggta attccaggtc aaaactgccg gtttccgcag gcttttggct
    19741 ggtaaaattg aaagcaggtg cccggcgtga taaaatcatc ataaaggtgc cctttgtact
    19801 tgcgcagcag taggttgtta tgaagaaaat gcgagaatgc ctgatttcaa gtgtacgcgt
    19861 cagcctgatt aaccaggacc gtatcgtggt gttgaaagag aaagaccagg aacgctacct
    19921 gccgatttgg attgggctgt acgagttcga ggcgataacc atcgctttgc acaaaattga
    19981 aaccgcccgt ccgctgaccc acgacctgat gaagaacctg tttggcgtcc tggacgcgcg
    20041 cctgctcgcc gtggaaatta ccgcgctgga atcagacact ttttacgcca acctggtcat
    20101 cgagcaaggc ggcgcgcaaa aacgcgtgga ctgccgtcca tccgacgcgc tcgcgctggt
    20161 ggtgcgcacc ggcgttccgg tgttcgtgga cgagggcgtg ctcgacaagg cggcagtaat
    20221 cccggagctt gagattcatc ctgaagaagg cgccgcagag gcaagcgaag aagggacggg
    20281 cgacctctca ttgtttgaag aatttttcaa caagctggac gataccgatg aggatctgcc
    20341 cggtcccgtc aacccggacg acggtcccaa tccggttccc tgaattcctg tcatcctcct
    20401 cccagccaac atcaagacag gtggagattt ggagagcgaa tgagcgatca agattataac
    20461 tccggcagcg ccggggaccc cggagccaac cagctttttg accggaccat ggaagaagcg
    20521 gtcattggcg cggtactggt cacgccgatt aagtacctgg acgtttcctc cattctgcag
    20581 acggatgatt tttactaccg cagccaccag tggatttggc aggcgttcca acgcctgtac
    20641 tccagcgacc ctgccttcga tttactcacg gtaaagcagg aactggaagc ccaggggcac
    20701 cttgaagaag tgggcggggt ggaatacctg gccagcctga ccaatcaggt tccgtcctcg
    20761 ctgcacgcgg aagcttacgc gcgcaacatc agcgcgctgg ctgcccgccg ccgcctgctg
    20821 accgcagcca atgaagttgc caaactggcc taccgtaaag atatcaatgt ggatacggtg
    20881 attgaccagg cggaacaatc catcttcggg gtcagtcagg agcgctataa gcacgacctt
    20941 gtcccgatta aagatatcgc gggtcagtac tatgaccgcc tgaccgagat gagcctgcaa
    21001 accggagcgg tatccgggct ggagaccggg ctcaagtccg tggataaagt gctggacggc
    21061 ttgcagaaat ccgatttcat tatcgtggct ggtcggccgg gtcagggtaa aaccggcttt
    21121 ctcatcggga ttgctaaaca cgtggggctg cagctaaagc gcaatgttgc catcttttca
    21181 ttggaaatgt cttctgaaca gctgctgcag cgtttgcttg cgcaggcgac ggatatcgac
    21241 tcccagaaac tgcgttccgc caggctctcc gatgaggaaa tgaaccgctc cgtaagcgca
    21301 ctgggcgtgc ttgccgaggc caatatttac attgacgaca cgcctgccat caccccgctg
    21361 caaatgcgca cgaaatgccg gcgtctgcag atggaatacg gcttggattt ggtcattgtc
    21421 gattacctgc aattaatgtc cgcggaagtg aaaaccgaca accgcgtgca ggaagtttcg
    21481 tatatttcac gctacctcaa agtgctggcg cgtgagctta aagtgccggt catcgcggcg
    21541 gcgcagctct cacgctcggt ggaatcgcgc ccggataggc gcccggtgct ttccgacctg
    21601 cgcgagtccg gcagtctgga acaggacgcg gacatcgtta tgttcatcaa ccagcctaac
    21661 caggaggaga gcagtccatt ccataccata gccaggttgg aaatcgcaaa acaccgcaac
    21721 ggcccgaccg accccgggat agaactgatt ttccagaaac gtctggcgat gttccgggac
    21781 cagtattacg gcgcggtgcc ggattaagcc tgccgcgatg agcgcttctc tcccttccgg
    21841 tggttttggc agcgacccgg gcacagcctg gtatctgccc gcggatctgc tggaacgcgc
    21901 tgccgaaacg tccctttccg cggacggtat gttggcagtt ctggcgtact tccggctgtt
    21961 gggccagcct gaagagggca gccaggcggt gcggcgggaa gcattggtcg aagcgctgag
    22021 cctgccgatt ttccgacagg atgccgcccg aacgcagctt gcgctggagg aagcgcttgg
    22081 ggcggggttc ttgataaagt acgaagatat ccttctagcg ggtacgccgc gcggaaaagt
    22141 cctgctcggg cagcttcagg ccggcgcaat cacggcagag cagctcaatc tggtcagtca
    22201 ggtgccagcc ccggaacgtc cgaatatttt ccggctgtac gaggctaata ttggccccct
    22261 gacgccgatg atggccgaaa tgttgaaagc cgacgcgctg gaattccccg cggaatggat
    22321 tgaggatgcc gtcagcgagg ctgtggaacg caatatccgc aactggcgct acgtgcgggc
    22381 catcctgaaa acctggaaag aaaaagggcg ggcggagaaa gcgagagaac ctgagggaga
    22441 tcttgaaaag taccgcaagc tctacgaaga ccaaaggcgc agaaaaaaac atgagtgatc
    22501 agcttcagaa cataacaaat gcggacctcc ccggcgaccc ggactgcccg ttctgccacg
    22561 gtgtcggctt tgtgggcaag gatgtgccgt tcgggcaccc ggagtttggc aaattgagcg
    22621 tgtgcgtatg ccgggcgcgc gagctggcag cagaccgcca gaaaaaactg tacgaattga
    22681 gcaacctggg cgccctgcag gaaaaaactt ttgagaactt caagccgcgc gggcgcgtcg
    22741 ggctggcaac tcagcaagcg aattcgctgg agcaagcgta caacagcgcc gcgcacttcg
    22801 ccgggacgct gcagggctgg ctgctgctgc tcgggcgcta cggctgtggg aagacccacc
    22861 tggcagccgc gattgccaat aaagcggtgg ctttagggat ggaaaccctt ttcctgacag
    22921 tcccggacct gttggactgg ctgcgtttcg cctattccga cggagcctcc gagtcgttcg
    22981 aggagcgctt tgagaaaatc cgggatgtgc cgctgctggt gatggatgac ttcgggacgc
    23041 agaacgccac tgcctgggcg caggaaaagc ttttccagat tctgaattac cgctacatta
    23101 accacctggc gacggtaatt accagcaaca atccgctcag ttcctttgag ggcagaatcc
    23161 gctcgcgatt aatggacccc gagctggtca cgcgcgtgga aatcctggcg actgattacc
    23221 gcaatccggc ggatgacatc gggcaccccg agctttccag cctgaacctg cacgcgcgga
    23281 aaaccttcga aagcttcagc ttgcgccgcg acgaggccat cccttccacg gaccgcgcca
    23341 ccctcaaaga cgcgctggaa gccgcccagg ctttcgcgcg cgacccgcag ggttggctgg
    23401 tcttcatcgg accttacggc tgtggaaaga cccacctggc ggccgccatc ggcaattacc
    23461 acgcgcagct gggcagcccg ccgctgatgg tggtggtgcc ggatttgctg gaccacttgc
    23521 gcgcggcgtt cgcgcccaac agttctgtca gcctggacca gcgctttgag gaaattcgca
    23581 gcgcgcggct gctgattctg gacgacctgg gcacccaatc cgccacgccc tgggcgcgcg
    23641 agaaaatcta tcagctgttc aactaccgct ataacgcgga gcttccgacg gtcatcacga
    23701 cggtgaacgc cccgggtgag atggacccgc gcctgcaaag ccgcctctcg gatgcccgcc
    23761 tgtgcaaggt ggtgattatg agcgcgccgc cttataccgg tggcggaaag gggcagggcg
    23821 gccgcgcgct ccggtaagcg ctttgccgct tggggggggg tctcaacaag ccttctattt
    23881 gtggggccag cctggtgtgc cttcggaccg ggattcggat tttctccagc cgacagcgcg
    23941 gaagcggtct tcgcagcggc ggagatgatg cgggctttgc tttggtcatt cccggtaccg
    24001 gcgaagcaac ccaggcagaa catcaaagtt cttctgacgc gctcatgaag gatgcttcgc
    24061 gcctatgccc gaacggcgtt cattcgtcca cgcgagaaga ggatgggccg ggttttctac
    24121 atgccgcccg accatttcca aacctctggc ttgcggaaaa aatcgtcggc gaccaaaaaa
    24181 cgtatccagt cagagcttag aaacagccgt ataattcccc tctgaggaaa actcctgccc
    24241 gaacggagcg cagaacttta cccaaaggcg cgcttgttcg gaaggatgtc atccccggcg
    24301 atccaaaatc ccgctcacat cagcttcagg agagaaaatg ggcacgattg acaaacgcga
    24361 gtctcagcat cctcaaggaa aaaacgcgct cccttccggc agcgaaagta aagctgccca
    24421 gaagctcgtt agcgttgccc atctggccgt tcaggacgta ctccagcagc aaaaaagcca
    24481 gctggagggc ctgacggaaa gcgaggtgtc agcgcgtctg gaacagtacg gctacaacga
    24541 ggtcgtcaag gaaaaacaac tggggctggt tctccgcctg ctcgggatgc taaaggaccc
    24601 gctcaccatc ctgctgatag tactcgcgat catttcgatc atcacgcacg atatccgcgc
    24661 ggcggtagtc atccttacga tggttgtact gggggttacc ttgcgtttcg tccaggaaag
    24721 ccgtgctgat tccgccgcgg aaaaactcaa ggaaatggtc agcacgatgg ccacggtaat
    24781 acgcgcaggt caggcgaagt ccatcccgct gcgcgagctg gtccctggag atattatctc
    24841 cctctccgcg ggcgatatgg tcccggcgga tgtacgcgta ctggttgcca aggatttatt
    24901 tgttaaccaa tcggcgctga ccggcgagtc aatcccggtc gaaaaatggg aagtaccggc
    24961 agacccgggc atcacaaacg cgctggagct gcccaatatt tgcttccttg gctccaacgt
    25021 ggaaagcggt accggcagcg cgctcgtcat ccacaccggt aaggacacct acttcggttc
    25081 cctggccagc agcatcaccg gcgagcgtca gaataccagt ttcgaccagg gcattaacaa
    25141 atttacctgg ctgatgctgc gctttatgat gatcatggtt cccgcggtgt tcctcatcaa
    25201 tggcttaagc aagcacgact ggctgcaggc tctcttgttc gcggtcgcgg tcgcggttgg
    25261 gatgaccccc gagatgctgc cgatgatcgt cacaaccaac ctctcgcagg gggcgctggc
    25321 catgtcgaaa aagaaggtga tcatcaagcg tctgaattcg atccagaatt ttggcgccat
    25381 ggatgtgctc tgcaccgata agaccggcac catcacccag gggaaaatcg tcctggagaa
    25441 gcatattgac ccgcgcggca atccaagcga aaaagtgctg gaatttggct atatcaacag
    25501 ctacttccag acaggcctca aaaacctgat ggacgaagcc atcctgcagc atgacgagca
    25561 ccgcgacgag atccaccgga aatggaagaa aattgacgaa atccccttcg actttaaccg
    25621 aaggcgcatg tcggttatcg tcgaagatga gaatggttta aacatgctcg tctgtaaggg
    25681 cgcggtggaa gaagtgatgg ggctctgcag ccatgtggag gcagatagcc aggtgctgga
    25741 agttctgccc gagcacacgg agcaccgcaa gaagcgcgtg gccgacctga atgcggacgg
    25801 cttccgcgtc gtggcggtag cctacaagtt gatgccaggc gcaccggacg agcctgtcta
    25861 cacggtgaat gatgagagcg acatggtttt agccggattt ctcgctttcc ttgacccgcc
    25921 taaagagacc gccatggaag cgattgagaa actgcatcag ctgaaggtgg aggttaaaat
    25981 cctcaccggg gacaatgatg tggttacgcg gcgcatttgc ggccaggtaa acttgcccgt
    26041 ggagcatatt gtgcttggtt ctgaactgga cgcgatgacg ccggaccaat tctcaaagac
    26101 ggtggaagaa aacgcagtat tcgccaagct ttcccccgcg cataaagagc tgatcattgc
    26161 cgcgctgcgc cagaacgagc atgtggtggg ttttatgggg gacggtatca acgacgcgcc
    26221 cgctctgaag gccgcggatg tcggcatttc ggtggattcg gccgtggata ttgccaaaga
    26281 gtcgtccgat atcatcctgc tggagaacaa tctgctgatt ttgcagcaag gcgttattga
    26341 aggccgcaag gtgtttggca atatcatgaa atacatcaag atggcggcaa gctcgaactt
    26401 cggcaacatg ttcagcgtgg tgggcgccag cgcgtttttg cccttcctgc ccatgctgcc
    26461 aatccaggtg ttggtcaaca acctgttgta cgacttctct caaacgacca ttcccaccga
    26521 tgaggtcgat caggaatggc tggtcaagcc gcggaaatgg ggcatccaag gcctggaacg
    26581 cttcatcctg ttgatcgggc caatcagttc catctttgat tacctcacct ttgcactgat
    26641 gctgtttgtc ttccacagca aggataatcc tgccttgttc cacactggct ggttcgtgga
    26701 aagtttgttt acccaaacct tgattatcca tgtcatccgc accaacaaga ttccctttat
    26761 tcaaagccgg gcaagcacgc cgttaatgat ttcctcgctg ctgattgtat ccatcggagc
    26821 attcctgcca ttctcaccgc tggccagcac gttgggcttt gtgccgctgc cggcggcgta
    26881 ttggggctac ctggcgggga tgctggtctg ctatgtcgcg ctgacacaac tggcgaagac
    26941 ctggtttatt cgcaagttcg gtgagtaaag ccggcaagcg ccagaggttc ggggaaattt
    27001 ccctgaccaa tgcttctgcc ctcccataag agagccaagc gcgcttgacc aggcgcagct
    27061 tgaggcgggt ctgaaccagg gcatacgtta ttggcagcag gggagtgccg cgctgctatc
    27121 gagttaaggc gctccgcaag agtaagccgg aaaaggcatt cgccagccgt acggctgcag
    27181 cgccggcata aaatcccgct ttgtgttatc atttgtacgc ttaaaaacca atgaattcag
    27241 aaacctgcgt cgagcaggct gttctatgtt aaaagtgaga gttatgaaca aaatcatccg
    27301 ggaagtgccc aatcatttca accttcctta ccgtctcaag cgcctggctg acctggcgca
    27361 taacttgtgg tgggtctgga accctgaagc cgcgcgcctg ttcaaggata tcgacccgct
    27421 cggctgggac aactgcaacc acaacccgat tgtcttcctg cgcaccgtga acccggtcgc
    27481 gctggaaaac ctgacccagg accgctattt cctggaccgc tacgaccgca tcgtacgcga
    27541 ctttgactac tacatgaaaa ccggcaagac ctggtacgct cagaatcacc ccgagctcct
    27601 ggaggacgaa atcgcgtact tctcgttcga gtacggcctg cacgaatccc tgatggtgta
    27661 tgccggtggt ctgggtgtgc tttccggcga ccacctgaag gaagccagcg acctgggcat
    27721 tccgctggtg gcggtcggtt tcctgtacac gtacggctat ttctcccaga aaattactga
    27781 ggatggttgg caggaagccc gccacgtcca aattatctat gaagacctgc ccctggttgc
    27841 gctttatgat gaaaaccacg agccaatcaa gatttccatc gatttgcccg gccgcaaagt
    27901 ttttgcccgg ctctacgagc tgaacatcgg ccgcgtcacc ctggtgctgc tgcagaccaa
    27961 catccccgaa aacaacccgc aggaccgcca attgactgac cgcctgtata tcagcgaccc
    28021 cgagctgcgt atttcccagg aaattctgct gggcatcggc ggtttgcgcg cgctgcaggc
    28081 gctggggcat aacgccagca tctatcatat gaacgaaggg cattccgctt tcctgaccct
    28141 ggagcgcatt tcccagctca tgcagtctgg caaatcctac tccgaagcgg cagaccaggt
    28201 gaagcgctcc agcattttta ccacccacac gccggttccc gctggcaacg acgaattccc
    28261 catctggctg attgacaagt acttctccag ctattggccg cagctcggat taaaccggga
    28321 tgagttcatg aatatcgcct tcgtacacca ggaatggggc ggagaagcct tcagcatgcc
    28381 ggtgctggcg ctaaaacttg cgaactaccg caacggcgtt tccaggatcc acggcgatgt
    28441 ttcccggcat atgtggcagc agctttggcc ggatacgccc gtggataaag tgccgatttc
    28501 gtacatcacc aacggcgtgc ataccggcac ctggctcgcg cggcgttatg ccctgctttt
    28561 caaccgctat ttgggctcgg actggaaaga acacattgat gacaaggaaa cgtggcagcg
    28621 catcgatgaa attccagatg aagacatctg gcgcatccgc ctgcacctca agcgcaaact
    28681 ggtggcgtat atggtcagcc gggcgcgcac gcagtggaag accaccaccg tgcatccggt
    28741 gcagaccgtt gccagcggcg tgctgctgga cccgtacgcg ctgacgattg ggttcgcgcg
    28801 ccgctttgcc acctacaaac gcgccaatct gctcttcagg gatgtggacc ggctgctgcg
    28861 catcgtcagt aacgaacgca aacctgttca gattgtcttt gccggcaagg cgcaccccgc
    28921 ggatgaaccc ggaaaacgcc tgatccagga ggtttaccag cacgttaagg atgcgcgctt
    28981 tggcgggcgg ctggtctttt tggatgatta cgacatggat atggcgcgct acctcgtcca
    29041 gggcgtggac gtctggctga atacgccccg acggccgcgc gaagcatccg gcaccagcgg
    29101 tgaaaaagcc gcgttgaacg gcaccctcaa tttctccgtg ttggacggtt ggtgggccga
    29161 aggctataat ggcgcgaacg gctgggctat cggcgatgac accgaatacg ccgaccagga
    29221 ccagcaggat aacgctgacg cgcagagcct gtataatacg cttgagaacg aaattgtgcc
    29281 gctctactac accaagcgct cagcggacgg actgccgggc gattggatcg ggcgaatcaa
    29341 ggaaagcatc aaatcgctcg cgccgcaatt ctcgatgacg cgtatgctta aggaatacat
    29401 tagcgacctc tatgtacccg cgatttctac ggccaaagag cccccgcaag agtaagaggt
    29461 cagaaggata attaattatg gatttcctcc aaaacctttc tcccatcgct ggcgcgttga
    29521 tgattttcct cctgcgcctg attaactaca cgatggatac actgcgcatt atgttgacca
    29581 tgcgcgacaa aaaactgatg tcctggatac tgggcttctt cgaatccatc ctgtttgtgg
    29641 tcgtcatggg cgctgtcctg gacgacctgg acaacatact caaggtcgtt gcctatgctg
    29701 gcggctttgc cacaggcaat gtggtgggca tgctgatcga gaaacgcctg gcaattggct
    29761 actctcacat cagcatcatc agccgcggtt tggggaagga aatttcccag gttttgcgcg
    29821 cgcacgattt tgccgtgacc gaaatccccg cttacgggcg cgacggcaag gtagccttgc
    29881 tgaactgctc ggttcagcgc aagctcgctg cggaggtgga aaaactggcg ctcgaacagg
    29941 acccgcaagc gttcatcaca attgaagacg tcgtcccccg ccagcgaggt tactggggca
    30001 gggggggaat caaccgctaa acaagaccgg cggcacttcg catgcaaaat ttgattggcg
    30061 cgctcatctt cgactttgac ggactcatcc tggatacaga gagccctgaa atacagattt
    30121 gggagcgcct cttcacggag gcaggcggac gctttgacca tcaagcttat cttggcacca
    30181 ttggcagttg ggataattcc agttacgaac ccggcgtgca tcttgcccgg ctgatggatc
    30241 acggcgtgac cccacaggaa ttagtcgcac gtgtccgtat ggaatctgcg gagtgcatcg
    30301 cacaggaacg gccgctgcca ggggttgaaa ggctcatcgc ccgggcgcgc ggggcagggc
    30361 tgcgcctggc gcttggttcc agttcgccgg ccgcgtgggt gcaggggcat ctgcgccggt
    30421 tggggttgat ccagaacttc gaaatactcg tgacctttga cgacgtacgc caaagcaagc
    30481 cttcccctga aatcttcctg accgttttgg cgcgcctcgg gattggagcg gaacaggcgc
    30541 tggtgttgga agactcttat aacggagttc tggcggccaa ccgggcagga atccgcgcgg
    30601 tagcggcccc caatgctgtt accgctgcac aggatttctc cctggcggag gaagtgttgg
    30661 acactttgga cgggctggtg ttgggcaagt atttcccgtt agctgactga taaatctacg
    30721 aaggtgtgct acgttataga cagttaagaa cattaacaaa acaggtctta tgtggcggag
    30781 tagaattgcc actttgacat acccctactt tatgcaatgg gcgacatgtg ggttagttac
    30841 ctgctgtggc tcgcatacgg tcgaagcagg tggaaacacc tgataggcca ctacttgtta
    30901 ctcccaagag tggcttttgg tttacattga tattaatatt gccccttgac atacaacttt
    30961 tcctccctga cgcggccatg agacggccgc gtttttgttt tgggtgtcgg aacgcggtca
    31021 ttatgttctg tctggacgcc ttttgtgcaa accgaaattg aacctcgcag ggcgtaggtt
    31081 gggtggagcg acgcaggcgc gaatcccaac aataataccc aaagaacacc ccgcatttcc
    31141 cgctaccaaa ttttgttggg ttgcgctcca aaaaataatt tgctgattag gtcatgctga
    31201 cgctccagct caacctgtaa ctgcccatat aggttacact atgcacttcg cgtgtccacg
    31261 tcgaagctcc taaggttaat caggacattt ctatcttgtt tcttgaggac atgatcatgg
    31321 cgcttttcca ttccaataaa tccaataaat caaaaatcca ataaatccaa tccaataaat
    31381 ccacaaaaag cggctgcccc acgtgctata atttccggca tgaacgtgct cgtcagcggc
    31441 gcagccggct tttgggctta ccatgatacg ctggagctgc tcgcgagcag gcgacagctc
    31501 aacgtgttgg tcctggcgag ccaagcggcg gacgcggttg tgctggcgct ggaacaggct
    31561 caagcccgtg ctcgcggccc ggtttgcgcg gagaacctga tctggcggga atttctgcag
    31621 cgcctgggca agattctggg caaaccaaag ccaatcctca cgctgcccaa agtgctggag
    31681 agcttaggcg cctggtttat
//
LOCUS       DAOQAD010000012        30916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_1412_length_30916_cov_5.318979, whole genome shotgun sequence.
ACCESSION   DAOQAD010000012 DAOQAD010000000
VERSION     DAOQAD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 30916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30916
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1412_length_30916_cov_5.318979"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..491)
                     /locus_tag="PKL60_02010"
     CDS             complement(36..491)
                     /locus_tag="PKL60_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94941.1"
                     /translation="MNTKDKSRIPGWVYLTLVINLTVFIFLGKFIKGDFSSKVGPTIT
                     PSPQSFIDRLSFPVIPTIPPGFLETYTLNLQKTHVRVNFPSGCPNGCTEYKKGCDIKG
                     NIAFETKEKIYHVPDQKYYDETIINADFGERWFCTEDEAIANGWRKSYE"
     gene            1003..1266
                     /gene="rpsT"
                     /locus_tag="PKL60_02015"
     CDS             1003..1266
                     /gene="rpsT"
                     /locus_tag="PKL60_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNW94942.1"
                     /translation="MANIKSQIKRNKQNQGKRQRNRVYRGAARTYVRKAQAAIKSGVE
                     VNAQAEVKNAIKALDKAVTMGVIHKNNAARRKSRLVKKLNSLQ"
     gene            1382..3133
                     /gene="argS"
                     /locus_tag="PKL60_02020"
     CDS             1382..3133
                     /gene="argS"
                     /locus_tag="PKL60_02020"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNW94943.1"
                     /translation="MFEQEKELLDSLLAEFCQERSLPADKLEWRSIPFSGEWGIAAPL
                     FPLAAADPARQLPVPQHAQALAEALRDSIQLPAGFSRLEAVRGYLNLYYETSAYARKV
                     IEGVLAAGKRYGCREQTGKPVMVEYSNPNTHKPLHVGHLRNVILGGSTCNILEASGKR
                     VIRANYLGDIGLHVIKWLCNYEKYHAGEKPGADKTRWMGDLFTEADRRFAYEEGFEAE
                     VRAYFARWDARDPHILALWKETRDWSLEGFRQVYDLLGEHFDRIYFESEVEEPGKAAV
                     DQLIKSGLAEDGRPNLPVVVNLDQILGTNEEYRVVIILRSDGTSLYATKDIPLAVMKF
                     NEYDLEKSIYVVDVRQSLHIKQFRKILELMGYPWAERIHHLAYEIVNLPGNVTMSSRE
                     GTVVLLDDLIREATRRALEVVREKNPELEPARMHEIAEAVALGAIKYPMLSRENTKMV
                     TFDWESALDFNGQSAPYIQYAHVRANSILRKADGLTATQPDYGRLTHASEVDLIERIA
                     RLPEEIQRAAKELKPNLLANYAYELAKGFNDFYNQCPVLGAEPELRAARLALVAAARW
                     CIGNTLRLLGIRAPEVM"
     gene            3177..4496
                     /locus_tag="PKL60_02025"
     CDS             3177..4496
                     /locus_tag="PKL60_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HNW94944.1"
                     /translation="MSRIRTIIMGAAGRDFHNFNTFFRDNEKYEVVAFTATQIPNIEG
                     RKYPAALAGGLYPNGIDIYPEAELPRLIKELKADEVVFAYSDVPHSYVMHKAAEVNAL
                     GPDFRLMGTAVTQVKSIKPVIGVCAVRTGSGKSQTTRKVAAILGKMGYKVAAIRHPMP
                     YGDLVKQKVQRFATYEDLDKHECTIEEREEYEPHIDAGVIVYAGVDYEAILREAEKEV
                     DIILWDGGNNDFSFYATDLLIVVADPHRPGHESEYYPGETNVRMADVFVINKVDTANP
                     DSVIAVRDNLFRLNPKAIVVEGASPIFVDDPEAIRGKRVLVVEDGPTLTHGGMAYGAG
                     YVAARRYGAAEIVDPRPYAVGSILDTYKKYPGTGQILPAMGYGEQMVRDLEKTIENAD
                     VDMVIIGTPIDLTRVLKLNKPSQRVRYDLEEIGKPTLTDILSAKFGK"
     gene            4609..5868
                     /gene="obgE"
                     /locus_tag="PKL60_02030"
     CDS             4609..5868
                     /gene="obgE"
                     /locus_tag="PKL60_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNW94945.1"
                     /translation="MFIDSVTITVQSGKGGDGLVHSHREKYRPRGGPDGGDGGKGGSV
                     ILQVVPTLNTLNKFHHNEIFSAENGKNGGPSNQSGKSAPDLIIPVPPGTMVYDDETTQ
                     LLGDLVNPGQTLVVARGGRGGRGNQHFASSRNQMPLTAERGEPGEEFVLRLELKLIAD
                     VGLVGMPNAGKSSFLAATTNARPKIANYPFTTLEPNLGVAELDEENSIVMADIPGLIE
                     GAHEGIGLGYDFLRHVQRTRVLIHILDGLSEDPYEDFLTINQELELFDEHLGKLPQIV
                     VFNKMDLPDAAAKFEGLKAKFAQEGRELLSISAVAHTNLKTVLWKAQEALKNAPIEET
                     EPELPVYRAEEDPKAFTVTKEDDLWVVRGKIIERAAAMTFWDQPGSVRRFQRLMTGLG
                     VEKALRQAGIEEGDTVSIGEYELEWQD"
     gene            complement(6084..6737)
                     /locus_tag="PKL60_02035"
     CDS             complement(6084..6737)
                     /locus_tag="PKL60_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNW94946.1"
                     /translation="MTPRLNSKKFVLMAGNIGSGKTSLTRLIGEKLGWQSAYESVADN
                     PYLADFYKDMRQWSFHLQVYFLGSRAAQHLELTHSPRSVIIDRSIYEDARIFARALNT
                     MGNLPDREYQAYLRLYDLIVNTLPKPDLLIFLRAPVSVLLKRIRIRARSIEDSIDPDY
                     LALLERYYDEWIDRFDLCPVLTIESDAVDFVNKPEQLELVSQRMLDRLAGTEKLVLD"
     gene            complement(6739..7572)
                     /locus_tag="PKL60_02040"
     CDS             complement(6739..7572)
                     /locus_tag="PKL60_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNW94947.1"
                     /translation="MPAENWTEGEYLTRGHKLAYVRTGAKLPPLILLHGFTEDSSTWF
                     ELAASLETAYDLIMPDMLGHGNSDRLTPGADIDLEQDLTDLITGLGLQKLALLGHSLG
                     ALTAAQFAAAHPELVACLILEDIPWFEPTALPAIPQDAYSAANPAVIARLAAGSRAQA
                     LAYCAEHFPHWNENARRAWAESKLRFDLEWYQRAPQKPTDWREISAKFNFPTLLVSGE
                     NKLGSMISSGFALTALKCIPRLEWARIPGAGHYVHYDAPVPFLSNVKTFLRMQYSPKK
                     G"
     gene            complement(7586..10150)
                     /locus_tag="PKL60_02045"
     CDS             complement(7586..10150)
                     /locus_tag="PKL60_02045"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNW94948.1"
                     /translation="MEHSATPPAGLLPTPPVPPDLPAITLSENSRQILAKRYLRRGED
                     GEPVESVEEMFWRIAWHIADVEAEAAPLETAKAFYRLLVSKSFFPNSPTFTGAGTPLG
                     QLAACFVLPISDDMGRASDGIFTTLRNAALIQQTGGGNGFNFSRIRPKGAIVKTSSGQ
                     ATGPVGFLKVYDNAFGEIAQGGTRRGANMGILRVDHPDIEEFISCKTNENAITNFNIS
                     IAITDAFMRAVEEDSPWELRFPDVSTPAYKTFSGTLEAAERAGLPIRTYKTVSARELF
                     KKFVAQAHHNGEPGALFLDAANRSNPVPHLYDLEATNPCGEQWLGPYENCCLGSVNLA
                     CHLAPDGKVDWEKLRESTILATRFLDHVVDKNAYVPAVPELRQSALNARRIGLGIMGL
                     ADLMYHAGVRYGSEESLEFAGQVMEFIRYHCMLTSIAMAKEHGPFPAIKGSIYDPENL
                     TWSAPEPLFPYTRDWGRPAVDWQLVFEGIHQHGIRNAAQTTIAPTGTIGTVAGCEGYG
                     CEPVFALAYVRHVNDHGRDLTLNYASPLFQSALDKAEFSPEQKEEIIREVIEAGSCQQ
                     IALLPEELRSVYVVSADIHAHEHIRLQAALQRFVDNSLSKTINFPAGASEQEVADTFL
                     EAWKLGCKGITVYITGSREKVVLETKATMQARQAAAEEKPETYGDAVVTQPSFWKDAK
                     KPRPRILKGYTYSIETPLGKAFITINENGGDQPFEVFINTAKAGSETAAHSEALGRLI
                     SYVLRIASSIEPRERLKIVMEQLAGIGGGRSLGFGPNRVRSLPDGIAKALDEYLFQRH
                     WEKEEEPEPLPGEPLPFMEDRKGVKSIGELCPECGQATLINEEGCRKCYTCGHSEC"
     gene            complement(10160..10618)
                     /gene="nrdR"
                     /locus_tag="PKL60_02050"
     CDS             complement(10160..10618)
                     /gene="nrdR"
                     /locus_tag="PKL60_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNW94949.1"
                     /translation="MNCPFCQFPDSRVIDTTKDSHGGIRRRRECASCHQRFSTMERAI
                     LATPMLIKRDGTREEFSREKLVQGLRIACAKRPVSAAALEQLASSIEAELQKKGRPEI
                     NSRIVGDLAIKGLKELDHIAYVRYAIVYLRMDDLHTVRNEIDHLLSEETK"
     gene            complement(11377..12540)
                     /gene="ftsZ"
                     /locus_tag="PKL60_02055"
     CDS             complement(11377..12540)
                     /gene="ftsZ"
                     /locus_tag="PKL60_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNW94950.1"
                     /translation="MDAKQTEINARIKVVGVGGGGCNAINRMIAEGLSGIEFIAINTD
                     AQALLKSSAPTRVRIGEKTTRGLGAGGDPKIGQAAAEESAEELYEVLKGSDMVFVTAG
                     LGGGTGTGAAPVVAQIAKEIGALTIGVVTRPFSFEGNHRSKAAEDGAMRLKEHADTLI
                     VIPNDRLLQIVTKNVALQDAFRIADDVLRQGIQGISELITVPGLINLDFADVRTIMSE
                     GGAALMAVGEASGEDRARIAAGQAISSQLLDVTINGARGIIFNMTGGSNFSLYEVYQA
                     ADIIKETAHPDVNLIFGAVVDESMGDKVRITVIATGFDHAATPAEVENNAFFHSAAPR
                     KQEKITAAEFPRRQEPAPKAQAPVEDYSFASGVDIDVPSFLRKRREQQLGSNG"
     gene            complement(12565..13803)
                     /gene="ftsA"
                     /locus_tag="PKL60_02060"
     CDS             complement(12565..13803)
                     /gene="ftsA"
                     /locus_tag="PKL60_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNW94951.1"
                     /translation="MEEPIVVGLDIGTSKICTLVARVENGTNLRILGVGIEPSQGIRR
                     GTVVDINSASQAIARSIEKAERTSGYEINSALVSLAGSHVTSMNSRGVVGISGRVIDQ
                     DDVYRALDSAQAVAVPHDREIIHVIQRGFTVDGQNGIRKPIGMHGFRMEVEAHIITAS
                     ASTVENLRQCVSMAGIEVSQFVLNPLAAGEMVLSDSERAMGVAVVDLGGGTTNLAIYI
                     DGDVWYTNVLNVGGGHVTSDIAQGLRLPVEVAEEVKLHHGYAVLEEIPEDESFNIVSF
                     GSEQSRKVSRRELTDIIEARVEEIFMLVHKEIKRSGYDGMLPAGLVLTGGGSLLPGIR
                     KLATRVTGLPARIGKPEKMVGLTDQISTPAFATSVGLLQWAILMSESNQIASEHSGKT
                     RKPLDVKAEKIKSWFGKLLP"
     gene            complement(13805..14842)
                     /locus_tag="PKL60_02065"
     CDS             complement(13805..14842)
                     /locus_tag="PKL60_02065"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNW94952.1"
                     /translation="MAPRYENLTRADQVRARRSIEPARRKPIPQPEKYSKRNAESSAR
                     VTVRRSDLNMARPTSAAYTQRRRKYYLPTDKSGAEIRLPAMPALKLSWRIASALLAIL
                     AGIGIYLVSSSPTFQVSSINLSGAVRVSAQEILETIDLEGRPIIRVSPEQIREEIAAS
                     FPDIDYAQVTVTFPAVVNVAVNERIPAITWYENDSPLFWVDEKGYSFPIRGEASIPLA
                     VYANGEPPRPLGYQTVQEAAQAEAAAETETAAAPVEPAPSVDPHFVDMVLKLRAVIPA
                     GTPLLYDAENGVGWKDPQGWQVFLGTDPADIDMKLAEYQVIVAYLLERNLQPVFINLE
                     YLHAPYYRLEQ"
     gene            complement(14852..15955)
                     /locus_tag="PKL60_02070"
     CDS             complement(14852..15955)
                     /locus_tag="PKL60_02070"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNW94953.1"
                     /translation="MNQQKNNLALVFGGRSGEHAVSLRSSRSVRSVLDLDKYNVFEIG
                     ITLDGVWLSGENALSAFENGDTSSLQEVSLLSTDGKVGLYQREYEKLKLIAELDVIFP
                     VLHGTFGEDGTIQGLFEILNVAYVGAGVLASAACMDKAVCKDLLQNAGIPVLPYQVFS
                     RSQIADYLEEVIALAEAVSAYPLFIKPANLGSSVGISKAGSREELRAALLKAARFDRR
                     ILVERGIDAREIEISVLGNDYPLVSVPGEIVPEDVFYTYKDKYLSGDPETIIPAPFDV
                     EMTQKIQEYALRAYRAVDGAGLSRVDFLMDKHTGELFLSEINTMPGFTSISMYPKLWK
                     ASGLEYPQLVDRLITLGLERKADQDQTVRRYED"
     gene            complement(15936..16868)
                     /gene="murB"
                     /locus_tag="PKL60_02075"
     CDS             complement(15936..16868)
                     /gene="murB"
                     /locus_tag="PKL60_02075"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNW94954.1"
                     /translation="MSIPMRKLQDFFGARLQENVRMANYTTSRVGGPALGLVSVNTIA
                     ELRDAANALWSLDVPFKLIGSGSNILVSDSGYQGIVLLNHCHNIRINTQEEPPSIYAE
                     SGANLSNVARQSALRGLSGLEWAGSVPGSVGGAVYGNAGAFGSDISRSLVSIQALVRG
                     EGEMTLTAEELMFSYRSSILKREPKDLLILSARLKAERSERATAWKALLEMSEKRQAN
                     QPTGASTGSTFKNPPGDYAGRLIEAAGLKGMTDGHAQFSGLHANFIVNDGNSSAQEYL
                     KLIRTAQKTVKKKFGVQLELEIELIGDFDEPAKE"
     gene            complement(16865..18259)
                     /gene="murC"
                     /locus_tag="PKL60_02080"
     CDS             complement(16865..18259)
                     /gene="murC"
                     /locus_tag="PKL60_02080"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNW94955.1"
                     /translation="MKRVHLIGIGGTGMSGIAVVLLEKGYQVSGSDLAASPYFQMVSK
                     RGARVALGHDPRLALQADLIVRSSAVRDDDPEVAAARAAGIPVIKREAFLSELTWGKE
                     TIAVAGSHGKTTTTAMLICMLDALTYDPSFIVGAEIKELATNARWDIGRHFVIEADEY
                     DRMFLGLTPALSVVTNIEYDHPDCFPTPQDYLDAFKDFINRTVPGGKSILCADDPGVR
                     SLLKADLRTDIELLTYGFHEGCDYRIEDYVWDGRASHFSLRRSKSQRQAQVLGQFTLR
                     LPGKHNVANAAAALAVVDALGASAAGSASALASFAGTERRFDIVLESAGEVLINDYGH
                     HPTQVRATLGAARELYPTKKLWAVWEPHTFSRTESLQKEFAQALDLADKVMITKIYAA
                     REPDDGFSPQPIVDALPAEKADYIPDFTELVEALSRQLTGNDVVVFLSAGKGPQICAS
                     LRESILRRSGEVDL"
     gene            complement(18259..18780)
                     /locus_tag="PKL60_02085"
     CDS             complement(18259..18780)
                     /locus_tag="PKL60_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94956.1"
                     /translation="MISLSVLFYVLVALFVLIGAMRGFGKEIVVCASSILALFTIEVV
                     IPRIFGDASGSKRFWINSIVIVVCALAGYQTPNLRRFAENVRFQRGPFRDMLAGGLMG
                     GINGYILVSSLWFYMADAGYPFKAITAPDLATELGQSAAKILESAFPTYMHVPILYYM
                     VVIAFFLLIGAFV"
     gene            complement(18797..19867)
                     /gene="murG"
                     /locus_tag="PKL60_02090"
     CDS             complement(18797..19867)
                     /gene="murG"
                     /locus_tag="PKL60_02090"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNW94957.1"
                     /translation="MRLLICAGGTGGGVYPALTVSETLRNDAHELLWVGSEGGMEKAL
                     VERAGLPFKAVPAAGVHGVSLKKLPGNLLRLARGYHSSRRLLKQFNPDVLFFTGGYVA
                     IPMAVAGLRRRSVLFVPDIEPGLALKVLARFASRIAVSVEDSRRYFKHQEKITVTGYP
                     LREDLKKWTKAEAQAYFGFDPQRKTILFLGGSSGARSINQAVMEIAAELTREYQVIHL
                     TGHLDWDEIQKHTHQLGPRYQAFPYLHEVGAAMACADLVVSRAGASTLGEYPYFGLPA
                     VLVPYPYAWKYQKVNADYLVERGAAVLMEDRYLKANLLATIKDLMTDAQRLAEMSAAM
                     RALSNPDAALAISELLLEMAGA"
     gene            complement(19788..21032)
                     /locus_tag="PKL60_02095"
     CDS             complement(19788..21032)
                     /locus_tag="PKL60_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNW94958.1"
                     /translation="MGERAVTNEFVKPAARRNSASKLPFDVPLLLIVASLLVIGLLMV
                     YSTSLDASLRMGKEPTYIFTRQIMWVLLGIALAVGLSFLDYHFYRPFVLIALGVVLVL
                     LLAVLIRNDIRFNSSRALFEGSFQPSEFAKLGIILYLSFWLSSHRDTMNLLGDGLLPL
                     LFIVGLTAGMIVLQPDLSAAATVVLLGILLFFLAGGDLKKLLIFVVFVVGIGAVVVSI
                     SRTGQARISDYLSGLKDLTGSSYHVQRIYEAIIKGGLFGVGIGEADTKFTGLPLPHTD
                     SIFAVIAEETGLVGAFLVVALYVLLLWRGLVIARRAPDQLGSLISFGLISWIALEALM
                     NVSVIVGLFPFTGNALPLISAGGSSMLATMSSLGIVMNVARQGVAKESAERSQTSAIV
                     DLRRRDRRRSVSGANRFGNPQE"
     gene            complement(21014..22402)
                     /gene="murD"
                     /locus_tag="PKL60_02100"
     CDS             complement(21014..22402)
                     /gene="murD"
                     /locus_tag="PKL60_02100"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNW94959.1"
                     /translation="MKNWADKKVIVLGAARQGTALSRFLAGKGARVVLTDLHGPEALS
                     ADVLTLKDAGVELCLGSHPLELLEGADLVCVSGGVPLNIPFLQETVRRGIKLSNDSQI
                     FLEACPAPVVGITGSAGKTTTTALLGLMASKYFEMKQSPHRAWVGGNIGNPLIGQVGE
                     MHADDLVILELSSFQLELMTVSPQVAVVLNITPNHLDRHVTMQAYSAAKARILNYQHQ
                     GDVAVLNRDDAGSWALAAQLKSDLISFGFNRPEGNQNGTYIHKDSIYLQIDRQQLKMF
                     PLDWIQLRGQHNISNVLAACAAAAGASLALPAIQSAVEEFSGVPHRLEFVRNVRGADW
                     YNDSIATAPERTLAALQSFEGPLVLLLGGRDKDLPWEKLAEHVHSRVRHAVLFGEAAG
                     LISQALGEVKAGETLQSITRCASLQDAVQKAAELAREGDTVLLSPGGTSYDAFKDFEE
                     RGECFRQWVNAL"
     gene            complement(22405..23400)
                     /gene="mraY"
                     /locus_tag="PKL60_02105"
     CDS             complement(22405..23400)
                     /gene="mraY"
                     /locus_tag="PKL60_02105"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNW94960.1"
                     /translation="MGGTSLAIAISGFSFLLTVIWGSPFIRLLKLLKIGKIISMDVPE
                     SHLAKMNTPTMGGFMFLIPITILTVILNAVSLFGSRLFGSSIFVPLATLWLFALIGAV
                     DDWEGIRGPRRGLGMSARTKFTLQAVVAIAIAYSLKEILRVPNVFWPASEAPLNLGWW
                     YLPIAAFIIVAMANAVNFTDGIDGLASMICMTMFGVYGIIAIFQGQVYLARFCFCIVG
                     ALAGFLWFNAKPAELMMGDTGSQALGAMLAVVALMTGRWVFLPLIAIIPVSELLSVII
                     QVGYFKLTHGKRVFKKAPIHHHFEMLGWQENQIVIRFWLIGFIGAMLGFALTFLG"
     gene            complement(23400..24812)
                     /gene="murF"
                     /locus_tag="PKL60_02110"
     CDS             complement(23400..24812)
                     /gene="murF"
                     /locus_tag="PKL60_02110"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNW94961.1"
                     /translation="MLTFQHVLKALLGRAVGELDFKLPEAFIDSRAVSEGSLFFALRG
                     ERTDGHLYVDDAFARGASLAILEHPIESAYPIVELNAAELQFPERPPFSLLTDNVVAA
                     LQKLAAYWRAQFDIKVIGITGSVGKSSTKELTANVLSKRLVTLKNPGNMNNEIGMPLS
                     LLSLNAEHEVAVLEMGFYVPGEIKLLCEIARPQIGIVTNIGTVHAERAGSIEAIAEGK
                     SELVQALPPAPEGVAILNYDDERVLAMAAKTSARVFTYGLCPQADLWADQVESMGLEG
                     IRCRIHYQGEDFYVTAPLIGRHSVYTMLRSAAAALSLGFSWDWIFYALKTSNLQLRIA
                     TTKTASGALLIDDTYNASPDSTLAALNLLEDLTGRKVAVLGDMLELGQYEEDGHQRVG
                     IRAAQVADEIVLVGQRSLQTQAAALESGFAPNHIHWFADANSAARYLRETLKEKDIAL
                     VKGSHAMRMDLIVASLEVIS"
     gene            complement(24814..26592)
                     /locus_tag="PKL60_02115"
     CDS             complement(24814..26592)
                     /locus_tag="PKL60_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNW94962.1"
                     /translation="MNNPSWTRYILILLVFALVAGSVGARMVSIQNIPAAQTILENSK
                     KLVGVTQTIYPERGSIYDAQGRLLAGNETVYEVGLDLRSIAQPETIASVASSVLGLDY
                     SSTLVYAKTEPIEDEKLYIVLDSFVDKEKIAQLELIEEDYTNRDIGKNETRPSLEGLI
                     WTPYNKRSYPEGDMGANLLGFYAFLDRVEGRGYYGVEEAYNDLLAGTPKTEYTPFDPQ
                     KITSVDDIPPGASLVLTFDREIQAMVEQQLAEAIDWSGAESGTIVVYNPEDGSIIAMA
                     TTPRMDPNYYWTQGDVFPGSTPFNRPISETYEPGSVFKVITMASALDAGAVKPDTVFT
                     DTGSIMVGGTTITNWNYGAWGDQDMTGCLQHSLNVCLSWIATQLGAEQFYTYLKNFGF
                     DRNTGIDLGGESHWPLKLPGDNQWYEGDLATNSFGQGISVTPIQMAMAIGAVANEGRM
                     MAPHIVKAIIMDGHQYDVNPVIVGNPIKAETAETLTRMLVDSLEEESSVALVEGYTVA
                     GKTGTAEIPTPFGYTSGVTNTSFVGWGPAEDPKFLVYVWLEKPTISIWGSEVAAPVFS
                     KVVQNLVVLMDIPPDPIRLQASTEDK"
     gene            complement(26589..27071)
                     /locus_tag="PKL60_02120"
     CDS             complement(26589..27071)
                     /locus_tag="PKL60_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW94963.1"
                     /translation="MKTKNIIQAYRQAPWRMQLQWIGIFLLILVSIAAIAGVYLSLSG
                     KAAVTGRRIQKLEYQVSALELQINDLASQLAYASSARVMSERAKALDMVQRDPAQAVY
                     LEVPGYSAESFTLAPAPSANALPSPSILPEFTASLWDWLGDILAQLPGPAENTPEVQP
                     "
     gene            complement(27091..28041)
                     /gene="rsmH"
                     /locus_tag="PKL60_02125"
     CDS             complement(27091..28041)
                     /gene="rsmH"
                     /locus_tag="PKL60_02125"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNW94964.1"
                     /translation="MSAGMGDQPHHRPVLYQEILNLLAPHSPGRYLDCTAGAGGHSSG
                     ILEASAPAGKLLAIDLDPRAVALTKARLEPYGERAIVRQGSYLHSRDYLQELGWEAVD
                     GILLDLGVSSMQLDEADRGFSFRQEGPLDMRFDPGSGPSAADLVNRLEEKELADILWK
                     YGEERYSRRIARAIVRARPLRTTTELAALVRSALGRTNEAQDPATRTFQALRIAVNDE
                     LAVIAEAIPQLIALLHPNARIAVIAFHSLEDRIIKQTFQRESRDCICPPEQPVCTCGH
                     RASITIVTHHPVEAGEQEKKENPRARSAKLRVAQKLPKMA"
     gene            complement(28041..28484)
                     /gene="mraZ"
                     /locus_tag="PKL60_02130"
     CDS             complement(28041..28484)
                     /gene="mraZ"
                     /locus_tag="PKL60_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNW94965.1"
                     /translation="MFLGQFEHNIDAKGRLTVPSNFREQAPEGLFVTKGFDSNLIAYP
                     RDVYERMAENINELSVTDPESRALRRMFFSNTVELSYDSAGRILLPAFLRDAAQITTA
                     CKLVGNGDNVEIWSMEHWQAENQKNGGAEANSGRWAAYNISTRGG"
     gene            28776..29852
                     /locus_tag="PKL60_02135"
     CDS             28776..29852
                     /locus_tag="PKL60_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNW94966.1"
                     /translation="MTPPGILNDRYQLIESLGTGGMALVYKAKDLMLERYVAVKVLRE
                     DFSVDAAFRERFRQEAKAAANLSHPNIVTVHDFGLDQRHLFIVMEFVPGTDLKTRIRE
                     KGRFEVQEALDLMVQACQGVGYAHRAGLVHCDIKPHNMLVSPDGRLKVTDFGIARALA
                     TISPEERSDVVWGSPLYFSPEQAAGLPPSPASDVYSLGVILYEMLTGQLPFNSADAAE
                     LARMHREVQPVPPRVLNPGIPKVLEDIILKVLSKEPSARYRTADQLGRLLVGLQAMKS
                     GSAQPQVVTTPGASAVPAQTAPAPVPAPPDTPVYQTSPEALETDTAEKKSPDWGTLLL
                     WLIAGAAMFGLIPFWLYVYLTLRP"
     gene            29861..30523
                     /gene="rpe"
                     /locus_tag="PKL60_02140"
     CDS             29861..30523
                     /gene="rpe"
                     /locus_tag="PKL60_02140"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNW94967.1"
                     /translation="MLATISPSILSANLANLIQDLRECESAGADKIHIDVMDGRFVPN
                     ISFGPVIVRACKQATSLPLDVHLMIVEPEKHLDAFVEAGADAITVHYETCPHIHRTLQ
                     RIRDLGAAPGVTFNPATPVDGLRYLAGMFDLVLIMSVNPGFGGQKFIPSALTKVRDTR
                     QLLREIGSDAVIQVDGGIDASTIAAVYEAGGRDFVAGTAVFGHPGGISAGIASLRQAL
                     VK"
     gene            complement(30565..30750)
                     /gene="rpmB"
                     /locus_tag="PKL60_02145"
     CDS             complement(30565..30750)
                     /gene="rpmB"
                     /locus_tag="PKL60_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNW94968.1"
                     /translation="MAKCNHCGKSTSFGHNRSHSLVATRREFRPNLQRITVFENGKKV
                     RKVLCARCIKTLTKAYN"
BASE COUNT         6541 a         8656 c         9044 g         6675 t
ORIGIN      
        1 gccggatcgc ggataatgtc aacggtgaaa tagatctact cataagattt cctccagcca
       61 tttgctattg cttcatcttc cgtacaaaac catctctcac caaagtcggc attaattatc
      121 gtttcatcgt aatacttctg atctggaacg tgatatattt tttcttttgt ttcaaaagca
      181 atattgcctt taatgtcgca gcctttcttg tattcagtac acccgtttgg gcaaccactt
      241 ggaaaattca ctcttacatg cgttttctgg agatttaagg tataagtctc aagaaaaccc
      301 ggtggaattg tcggtataac cggaaatgag aggcggtcta tgaaactttg tggagatggg
      361 gtaatagttg gaccaacttt cgaggagaaa tcacctttta tgaattttcc aagaaaaata
      421 aacacagtaa gatttattac taaggtaaga taaacccaac cgggaatccg agatttatct
      481 tttgtgttca taacgttagg attcctgtgt agacccgttg tcagactctg ctaacttatc
      541 atcagcataa ttggatagta gaagtccaat aacagcaaga attatggaaa atgcacaaat
      601 aatcaatcta gtgtcaacac caaatttaaa aaaatagtaa attgggccta taaaggcgag
      661 gggtgataac ccaataagac agccgatggg tcctaataat tcttctaata tttttattcc
      721 aaggaaccaa taaatgaaac caaaaagata taattgaact tttcctacat aatacaatac
      781 cttcattttg aatttcctca gaattaattt ttgtgtagtt tatgtcaata agaaaaatca
      841 cctcatcttc atacaaatat tatatgatta tctacacaaa acgcaattgt ttgggctttc
      901 tgggtcgaaa tatgtaattt gagcttgtca gggctggaca atttatgatt ttgctgtaaa
      961 atatagtttc gtgcaattgg aattatcgtt cggaggaaga aattggcaaa tattaagtct
     1021 caaatcaaac gaaataaaca gaaccaggga aagcgccagc gcaaccgcgt gtatcgcggc
     1081 gccgcgcgca cttatgtccg caaggctcag gccgccatca aaagtggcgt ggaagtcaac
     1141 gcccaggcag aagtgaaaaa cgccatcaag gctctggata aagcggtaac catgggcgtc
     1201 attcacaaga acaatgctgc ccgccgcaag tcccgcctgg ttaagaaact caattccctt
     1261 cagtaggcgc tcaaacggct gtaacctgcg ggagccttcc ggctcccgcg tttgttgtta
     1321 agccgcgggg cgggcccggg cggaatgtta taatccagaa aactacgcac aggataggcg
     1381 catgttcgaa caggaaaaag agctcttaga tagtcttctc gctgagttct gccaggagcg
     1441 ttcgctgccg gcagataagc tggaatggcg cagtatcccc ttcagcgggg aatggggcat
     1501 cgcggcgccg ctctttccgc tggccgcggc agacccggcc cggcagctgc ccgttccgca
     1561 gcatgcgcag gcgttggcgg aagcgctgcg cgatagcatc cagctcccgg ctggcttcag
     1621 ccgcctggag gctgtgcgcg gttaccttaa tctgtattat gagaccagcg cgtatgcccg
     1681 caaggtaatt gaaggcgtgt tggctgccgg aaagcgctac gggtgccggg aacagacggg
     1741 caagccggtg atggtggagt actccaatcc gaacacgcat aaacccctgc acgtcgggca
     1801 cctgcggaat gttatcctgg gcggttctac ctgcaatatc ctggaagcca gcggcaaacg
     1861 cgtgattcgc gccaactatc tgggcgatat tggtttgcac gttatcaaat ggctatgcaa
     1921 ttacgagaag taccacgcgg gagaaaaacc cggggcagat aagacgcgct ggatgggcga
     1981 cctctttacc gaggctgacc ggcgctttgc ctatgaagag ggcttcgagg cggaagttcg
     2041 cgcttatttt gcccgctggg acgcacggga cccgcatatc ctggctctct ggaaggaaac
     2101 gcgcgattgg tcgctggaag gtttccgtca ggtttatgac ctgttgggtg aacacttcga
     2161 ccgcatttac tttgaaagtg aggtggaaga accgggaaag gcagccgttg accagctgat
     2221 taagtccggt ctggcagagg acggccgccc gaacctgccg gtcgtggtca acctggacca
     2281 gatattgggc accaatgagg aataccgcgt ggtgatcatc ctgcgttcgg acgggacctc
     2341 gctctacgcc accaaggata ttcccctggc ggtgatgaaa ttcaacgagt acgacctcga
     2401 aaaatccatc tacgttgtgg atgtgcgcca aagtttgcat atcaagcaat tccgcaagat
     2461 tctcgagttg atggggtatc cctgggcgga gcgcattcac cacctggcgt atgaaattgt
     2521 gaacctgccg gggaatgtga ccatgtcctc gcgcgagggg acggtggtgc tcttggatga
     2581 tttaatccgc gaagccacgc gccgcgcgtt ggaagtggtg cgggagaaga accccgagct
     2641 ggagcctgcc cgcatgcatg agattgccga agccgtcgcg ctgggagcta tcaagtaccc
     2701 gatgctctcg cgcgagaata ccaagatggt gacctttgac tgggaaagcg cgctggactt
     2761 taacggccag tctgccccgt atattcaata cgcgcatgtg cgcgccaaca gcatcctgcg
     2821 aaaggcggac ggactgacgg caacccagcc tgattacggc agattgaccc acgccagcga
     2881 agtggacctg atcgaacgca ttgcgcgcct gcccgaagag attcagcggg cagccaaaga
     2941 actgaagcca aatctgcttg caaattacgc ttacgagctg gcaaagggtt ttaacgattt
     3001 ttacaaccaa tgcccggttt tgggcgccga gccggagctg cgcgcggcgc gcctggcgtt
     3061 ggtggcggcg gcacgctggt gcatcgggaa cactttgcga cttcttggca tccgcgcgcc
     3121 ggaagtgatg taaaataaac tgtcaataga accaccaata aaaaggagag catgttatga
     3181 gtcgtatccg cactattatc atgggggcag ctggccggga ttttcacaac ttcaatacct
     3241 tcttccgcga caatgagaag tatgaagtcg tggcgttcac agccacccag attccgaaca
     3301 ttgagggccg aaagtatccg gcggcgctgg caggcggcct ttacccgaat ggaatcgata
     3361 tctaccccga agcggaacta ccccggttga ttaaggaatt gaaagcggat gaggttgttt
     3421 ttgcttacag cgatgtgccg cacagctatg tgatgcataa agcggcggaa gtgaacgccc
     3481 tggggccgga tttccgcctg atgggcaccg ctgtcaccca ggtcaaatca atcaaacccg
     3541 ttatcggcgt ttgcgccgtg cgcaccggtt ccggcaaaag ccagaccacc cgcaaagttg
     3601 ccgctattct gggcaaaatg ggctataaag tggccgctat ccgccacccg atgccttacg
     3661 gcgacctggt gaagcagaag gttcagcgct ttgccaccta tgaggacctg gacaagcacg
     3721 agtgcaccat cgaagagcgc gaggaatatg aacctcatat cgacgcgggc gtgattgtgt
     3781 atgccggcgt ggactacgaa gccatcctgc gcgaagccga aaaggaagtg gacatcatcc
     3841 tgtgggacgg tggaaacaac gacttctcct tctatgcgac cgatttgctg attgtggtcg
     3901 cggacccgca ccgccctggg catgagtcag aatattatcc cggagagaca aacgtgcgca
     3961 tggcggatgt gttcgtcatc aataaggtcg atactgccaa ccccgacagc gtgattgccg
     4021 tgcgcgacaa cctgttccgc ctcaatccca aggcgattgt ggtggaaggc gcctcgccca
     4081 tctttgtgga tgacccggag gctatccgcg ggaagcgcgt cctggtggtg gaagacggcc
     4141 ccaccctgac ccacggcggc atggcttacg gcgcaggcta cgttgccgca cggcgatatg
     4201 gcgctgctga aatcgtggat ccgcgccctt acgcggtggg cagcatcctg gatacctaca
     4261 agaagtatcc aggcactggt cagattttgc ccgcgatggg ttacggcgag caaatggtgc
     4321 gcgacctgga aaagacgatt gagaacgccg acgtggatat ggtcatcatc ggtactccga
     4381 ttgacctcac ccgcgtgctt aagctgaaca aacccagcca gcgcgtgcgt tacgacctgg
     4441 aagaaatcgg caagccgacc ctaaccgata ttctaagcgc aaaatttggc aagtaaaccg
     4501 gcaaaagcat tctcaaacgc tgtttcaggg gtcaggcctg cctgacccct gtttttttgc
     4561 ccgctgatat ataatgaggg cggcaaacat gttcatgagg taacagggat gttcattgat
     4621 tcagtaacga ttacagtaca atccggcaaa ggcggcgatg gtctggtgca cagccaccgt
     4681 gaaaaatacc gcccgcgagg cggaccagac ggaggcgacg gaggtaaggg cggcagcgtg
     4741 attctgcagg tcgtcccgac gttgaacacg ctgaacaaat ttcaccacaa cgaaatattc
     4801 agcgcggaaa acgggaaaaa cgggggacct tccaaccaat ccggaaaatc cgcgccggac
     4861 ctgattattc cggttccacc cggcacgatg gtatatgacg atgaaaccac ccagctgctg
     4921 ggggacctgg tgaatccggg tcaaacgctt gtagtcgcaa ggggcggccg gggcgggcgc
     4981 ggaaaccagc atttcgcttc ctcccgcaac caaatgccgc tgactgcgga acgcggcgag
     5041 ccgggcgagg aattcgtcct acgcctggag ctgaaattaa ttgcagacgt cggcttggtg
     5101 ggcatgccca acgcgggcaa gtcctcgttt ttggcggcca ccacgaacgc gagacctaaa
     5161 atcgcgaatt atccttttac aacgctggaa cccaacctgg gcgtggcgga attggacgag
     5221 gaaaacagca tcgtgatggc cgatattcca ggcttgatcg aaggcgcgca cgaaggcatc
     5281 ggtctgggtt acgacttcct ccggcacgtc caacgcacgc gcgttttgat tcatatcctg
     5341 gacgggcttt ccgaggatcc ctacgaagat ttcctcacga ttaatcagga actggaactg
     5401 ttcgatgagc atttgggcaa gcttccgcaa attgtggttt tcaacaagat ggatttaccg
     5461 gacgccgccg cgaagttcga gggattgaag gcaaaattcg cgcaggaagg tcgggaactg
     5521 ctgtcgattt ccgccgtggc gcatactaac ctgaagactg tcctgtggaa agcgcaagaa
     5581 gcgctgaaga acgcgccgat cgaagagacc gagcccgaac tgcctgttta ccgcgcggag
     5641 gaagacccca aagcctttac cgtaacgaaa gaggatgacc tgtgggtggt gcgcggaaag
     5701 atcatcgaac gcgcggcggc gatgactttt tgggatcaac ccggctcagt gcggcgcttc
     5761 caacgtctga tgaccggcct cggggtggag aaagcgctgc gtcaggctgg catcgaagaa
     5821 ggggataccg tcagcatcgg cgagtatgaa ctggagtggc aggattaaaa tgttcaccga
     5881 ctgggtgagg ggggcaccca atcggtgaac ggcacaattt aaccatatcc gccaaatctt
     5941 gtcaaggtct tgttatcctg taattacagg tttagcttga aaaagggtgg ggaaccaggc
     6001 aatttcccgc gcttaacctc gcaattatcc ggctgatgtt gggccttcaa acgcgctttc
     6061 gggtatggta tgaccccagg gaatcagtcc aaaaccagtt tctcggtgcc ggccagacgg
     6121 tccagcatgc gttgggacac gagttccaac tgctcgggtt tgttcacaaa gtccaccgcg
     6181 tcgctctcga tggtgaggac cgggcagagg tcaaagcggt caatccattc gtcgtagtat
     6241 cgttccagca aggccaggta gtcagggtcg atgctgtctt caatggaccg ggcgcgaatc
     6301 cggatgcgct tgagcagcac gctcacaggc gctctcagga agataagcaa atctggtttg
     6361 ggcagggtgt tgacaatcag gtcgtacagg cgtaaatagg cttgatattc cctatccggc
     6421 aagttcccca tagtattcag cgcgcgcgcg aagatgcgcg cgtcctcgta gatgctgcgg
     6481 tcgatgatga ctgagcgcgg cgagtgcgtc aattccaggt gctgcgcggc gcggctgccg
     6541 agaaaataca cctgcaagtg aaaagaccac tggcgcatgt ctttgtaaaa gtcagccagg
     6601 tagggattat cggcaacgga ttcgtacgca ctttgccagc ccagcttttc gccaatcaga
     6661 cgggtcaggg aggttttgcc gctgccgata ttgccagcca tcaagacgaa ctttttgctg
     6721 tttagcctgg gtgtcatgct atcctttttt gggggagtac tgcatgcgta aaaaagtttt
     6781 cacattcgag agaaacggaa ccggcgcatc gtaatgcacg taatgccccg cgccggggat
     6841 acgcgcccat tccagtcggg gaatgcattt cagcgctgtc agggcaaaac ccgaagaaat
     6901 catgctgccc aatttatttt ccccactcac cagcagcgtc gggaaattga acttagcgct
     6961 gatttcgcgc cagtcagtcg gcttttgggg ggctcgctga taccattcca ggtcaaagcg
     7021 cagtttggat tccgcccagg ccctgcgcgc gttttcgttc cagtgcggaa agtgttccgc
     7081 gcagtaagcc agagcttgtg ctcggctgcc cgcggcgagc cgggcgatga cagccgggtt
     7141 cgccgcgctg taggcgtcct gcggaatggc gggcagggca gtgggttcga accaggggat
     7201 atcttccagg attaagcacg caacgagctc agggtgtgcg gcggcaaact gcgcggcggt
     7261 cagggcgccc agggaatgcc ccagcagcgc gagcttctgc aggccaagcc cggtaatcag
     7321 gtccgtcagg tcctgttcca ggtctatgtc cgcgccaggg gtcaggcggt cggaatttcc
     7381 gtgccccagc atgtccggca taatcaggtc gtaagcagtc tccagacttg cggcgagttc
     7441 gaaccaggtg gagctgtcct ccgtaaagcc gtgcagcagg atcagcgggg gcagcttagc
     7501 gccggtgcga acgtaagcca gtttgtgccc ccgggtaagg tattcgcctt ccgtccagtt
     7561 ttccgcaggc atagacctcc ttcagttaac actcactatg tccgcaagta tagcacttgc
     7621 ggcagccttc ttcgtttatc agggtggcct ggccgcattc ggggcacagc tctcctatcg
     7681 acttaacgcc tttcctgtcc tccataaagg gcagcggttc gcctggcagt ggttcaggct
     7741 cttcttcctt ctcccagtgg cgttggaaaa ggtactcgtc aagcgccttg gcaatcccat
     7801 ccggcagaga acgcacgcgg ttgggcccga aaccaagcga cctgccgccg ccaatgccag
     7861 ccaactgctc catgacaatt ttcaggcgtt cgcgcggttc aattgaagag gcgatgcgca
     7921 gcacgtagga gatgaggcgc cccagcgcct cggaatgcgc tgctgtttct gagccggctt
     7981 tggctgtgtt gatgaaaact tcaaagggtt ggtcgccccc gttctcgtta atggtgatga
     8041 aggctttccc aagcggggtt tcgatgctgt aggtatagcc cttgagaatg cggggacgcg
     8101 gttttttggc gtctttccag aaacttggtt gtgtgaccac cgcgtcgccg taagtttcgg
     8161 gtttctcttc agctgcggct tgcctggctt gcattgtggc cttggtttcc aacaccactt
     8221 tctcgcgcga gccggtgata tagaccgtga tgcccttgca gcccagtttc cacgcctcga
     8281 ggaaggtgtc ggcgacttcc tgttcactgg cgccggccgg gaagttgatg gtcttgctca
     8341 ggctgttatc cacaaagcgc tgcaaagctg cctgcagacg gatatgttcg tgggcatgga
     8401 tatccgcgga gacgacatac acgctgcgca attcttcggg cagcagcgcg atttgctggc
     8461 agctgcccgc ttcgatcacc tcgcggataa tctcttcttt ttgctcagga ctgaattcgg
     8521 ctttgtccag cgcagactgg aagagcgggc tggcgtaatt cagcgtcagg tcccgaccgt
     8581 gatcattgac gtgccggacg taggccagcg cgaagacagg ctcgcagccg tagccttcgc
     8641 agccagcaac cgtgccgatg gtgccagtgg gagcgatcgt tgtttgggcg gcgttgcgga
     8701 tgccgtgctg atggatgcct tcaaagacca actgccaatc tactgccggc cggccccaat
     8761 cgcgcgtgta ggggaacaag ggctcagggg cagaccaggt caggttttcg gggtcataaa
     8821 tgctgccttt gatagcaggg aaggggccgt gctctttcgc cattgcgata ctggtgagca
     8881 tgcagtggta gcggatgaat tccatcacct gcccggcgaa ttcaagcgat tcttcggagc
     8941 cgtagcgcac gccggcatgg tacatcaggt cagccaggcc cataatccct aacccgatac
     9001 ggcgggcatt cagcgcggac tgccgtagtt ccggcaccgc cggcacgtaa gcgtttttat
     9061 ccaccacatg gtccagaaaa cgggtagcca gaatcgtgga ttcgcgcagt ttttcccagt
     9121 ccacttttcc gtcgggggca aggtggcagg ccaggttaac cgagccgagg cagcaatttt
     9181 cgtagggtcc gagccattgc tcgccgcaag gattagtggc ttccaggtcg tacaggtgcg
     9241 ggacagggtt ggagcggttg gccgcgtcca ggaagagcgc gcccggttca ccgttgtgat
     9301 gcgcctgggc gacaaacttc ttgaacaatt cgcgcgcgct gacggttttg taggtgcgga
     9361 tcggcagccc ggcgcgttcg gcggcttcca gagtgccgga gaaggtctta taagccgggg
     9421 tgctcacatc cggaaagcgc agctcccatg gagaatcttc ctcgacagcg cgcatgaagg
     9481 cgtctgtgat tgcgatggag atattgaagt tggtaattgc gttttcgttc gttttgcagc
     9541 tgatgaattc ttcaatatcg ggatgatcaa cacgcaggat acccatattg gcaccgcggc
     9601 gcgtcccgcc ctgggcaatt tcgccgaagg cattatcgta gactttcagg aagcctacag
     9661 gcccggtagc ctgcccggaa gaggtcttga ctatcgcgcc tttggggcgg atgcgcgaaa
     9721 agttgaatcc atttccgccg ccggtttgct ggatgagggc tgcgttgcgc agggtggtga
     9781 aaattccgtc cgaagcccgg cccatatcat cggaaattgg cagcacaaag caggcagcca
     9841 gctgcccaag cggtgtgccg gcgccggtga aggtggggga gttgggaaag aacgacttcg
     9901 aaaccagcag gcggtaaaaa gccttggcgg tctcgagagg cgccgcttct gcttccacgt
     9961 ccgcgatgtg ccaggcaatc cgccagaaca tctcctcaac gctctccacc ggctcgccat
    10021 cttcacctct gcgcagataa cgcttcgcga gaatttggcg ggaattttcg cttagcgtga
    10081 tggcgggcaa gtcaggtggg actggggggg tgggcaaaag acccgcgggc ggggtagctg
    10141 agtgttccat tggacgtcct tatttcgttt cttcggaaag caaatggtca atttcattac
    10201 ggacggtatg caggtcatcc atccgcaaat agacaatggc ataacgcaca taggcgatat
    10261 ggtccagctc tttcaggcct ttgatggcga gatcgccgac gatgcgggag ttaatttcgg
    10321 ggcgtccctt tttctgcagt tctgcctcga tggagctggc caattgctcc agagccgcgg
    10381 cggagactgg ccgtttggcg caggcgatgc gcaggccttg caccagcttc tcacggctaa
    10441 attcttcgcg cgtgccgtcc cgtttgatta acatcggagt ggcgaggatg gcacgttcca
    10501 tggtgctgaa gcgctgatga caggaggcgc attctctgcg ccggcggatg ccgccgtgcg
    10561 aatctttggt tgtgtcaatg acccttgaat cgggaaattg acaaaatggg caattcatcg
    10621 cgtctccttt gctcgagtac cccaatatat ggcggtgtgg aaagttatct cccccacctg
    10681 tctggtgttg acattttagc acgctttttg cacgggagca agaaaaggga cggcgattat
    10741 cttaaaaaat gtaagacttt aaggttagcc atttttgttt gccggtttgg gcggagcgca
    10801 tccaataccg ctggaaacca gacaaaaatc aggcgctttt ctctcgttgg aggggggatt
    10861 gatacggaat ttgcgggaat tcctgccgaa agcgaggtaa tggcgcttgt taataattca
    10921 caacgaggta ttagcgctgc ttcccggaaa ggatgcggga tttcctctgc gttcaagctt
    10981 gccggctggc tgatgtggtt ttggcatgct gccaggtcca ttcggcgtgt ctttagggaa
    11041 aaaaaggagc cggcggtctt gccttcaggc cccagcggct gccgacttgg agtgttgatt
    11101 gtcgctgtac gcgcatagcg gctttctcaa tggctgtgct tgtgggcaat cattcaagag
    11161 ggctttaaca gtcactgcct ggcatccgcg tttgggtacg ggtgggcagg aagcccggca
    11221 tgcaggcaaa aaacccaact cccgttgttc caggcgctgc gcagcaaacg cgcagacaaa
    11281 agctcataag ccggcttgtt tcagcttcct tcctccgggt atcaccaggg aagaaggaag
    11341 ctgtttgctg caagcccaag aggaggtcag gcgagcttat ccgttcgaac cgagctgctg
    11401 ttcgcggcgc ttgcgcagga acgaaggcac gtcgatatcc acgccggaag caaaggagta
    11461 atcttcaacg ggcgcttgcg ctttgggcgc aggttcctgc cgcctgggga actcggcagc
    11521 cgttatcttc tcctgttttc tgggggccgc gctgtgaaaa aacgcgttgt tctccacctc
    11581 tgcaggagtg gcggcatggt caaatccggt ggctatgacg gtgatgcgga ccttatcgcc
    11641 catggactca tcgaccacgg cgccgaaaat aaggttgaca tcggggtgcg cggtctcttt
    11701 gatgatatcc gctgcctggt agacctcata gagcgagaag tttgaaccgc cggtcatgtt
    11761 gaagatgatg ccgcgggctc cattgatggt cacatccagg agttggctgg aaatcgcctg
    11821 tccggcagca atcctggcgc ggtcctcgcc tgaggcctcg ccaaccgcca tgagcgctgc
    11881 accgccttcg gacataatcg tgcgcacatc ggcgaagtcc aggttgatga gaccggggac
    11941 ggtaatcagt tccgagatgc cctgaatccc ctggcgcagc acgtcatcgg cgatgcggaa
    12001 ggcatcctgc agggcgacgt tcttggtgac aatctgcagc agccggtcgt tggggatgac
    12061 aatcagggtg tccgcatgtt ccttcaggcg catggcgccg tcttccgcgg ctttcgagcg
    12121 gtggttgcct tcaaagctga acgggcgggt gaccacaccg atggtaaggg cgccaatttc
    12181 cttggcaatc tgcgctacga cgggagcagc gccggtgccg gttccaccgc caagacccgc
    12241 ggtgacgaaa accatgtcac tgccttttaa cacttcatac agctcttcgg cggattcttc
    12301 cgcggctgcc tgcccaattt tggggtcgcc gccggcgccg agaccgcgcg tagttttttc
    12361 gccaatccga acgcgggtgg gcgcgctgga tttcagcagc gcctgcgcgt cggtgttgat
    12421 ggcgataaac tcaatcccgg atagaccttc ggcaatcatg cggttgatgg cattgcagcc
    12481 gccgccgccg acgcctacta ccttaattct ggcatttatt tctgtctgtt tcgcatccat
    12541 tttatttctc ctcttgacaa ataactacgg taacaatttc ccaaaccaac tctttatctt
    12601 ctccgctttg acatcgagtg gtttcctggt ctttccagaa tgctcgctgg caatctggtt
    12661 agattcactc atcaaaattg cccactgcag cagaccgacg ctggtggcaa aagccggggt
    12721 gctgatctga tcggtcaacc ccaccatttt ttccggtttt ccgattctgg caggcagacc
    12781 cgtgacgcgg gtggccagct tgcggatgcc cggcagcaga ctgccgccgc ccgtcaagac
    12841 aaggccggca gggagcatcc cgtcgtaacc ggagcgcttg atttccttgt ggacgagcat
    12901 aaagatttcc tccacgcgcg cttcaataat atcggtcagt tcccggcggg agaccttgcg
    12961 gctctgctcc gagccgaacg acacgatatt gaagctttcg tcttcgggga tttcctccaa
    13021 cactgcgtag ccgtgatgca gtttcacttc ttccgccact tcaaccggca gccggagacc
    13081 ctgggcgata tcggaggtga catgaccgcc gccgacattg agcacattcg tgtaccacac
    13141 gtccccgtcg atgtagatcg ccaggttggt tgtaccgccg cccaggtcca ccaccgccac
    13201 gcccatggcg cgctcggaat ccgagaggac catctcgccg gcagccaggg gattgaggac
    13261 aaattgcgaa acttcgatgc cggccatcga gacgcactga cgcaggtttt cgacggtgga
    13321 ggcggaagcg gtgatgatgt gcgcttccac ttccatcctg aagccgtgca tgccgatagg
    13381 cttgcggatg ccgttttgcc cgtcaaccgt gaagccgcgc tggatgacat ggatgatttc
    13441 ccggtcatgg ggaacggcta ccgcctgggc ggaatccagg gcgcggtaga cgtcgtcctg
    13501 gtcaatcacc cggccggaaa tgcccacgac gccgcggctg ttcatggagg tgacatgcga
    13561 acccgccagg ctcaccaggg cgctgttgat ttcgtatccg gaagtgcgct cagccttttc
    13621 gattgaacgc gcaatcgcct gggatgcgga attaatatcc acgacagtcc cccggcgaat
    13681 tccctgggaa ggctcaatgc cgacgcccag gatgcgcagg ttcgtgccgt tttccacgcg
    13741 ggccaccagg gtgcagattt ttgaggttcc gatatccagt ccgacgacaa tgggttcttc
    13801 catcttattg ctccaatcga taatacggtg cgtgtaagta ttcgagatta atgaaaacag
    13861 gttgcaagtt tctttccaga aggtacgcaa cgatgacctg gtactctgcc agcttcatat
    13921 cgatatccgc cgggtccgtg cccaggaaaa cctgccagcc ctgggggtcc ttccacccga
    13981 cgccgttttc agcgtcgtag aggaggggcg ttccggccgg gattaccgcg cgcagcttga
    14041 gcaccatgtc cacaaagtgc gggtcaaccg aaggggctgg ttccaccggc gcggctgcag
    14101 tttcagtctc tgccgcggct tctgcctggg ctgcttcctg cacggtctga tagcccaatg
    14161 gtcggggcgg ttcgccgttg gcatagaccg ccagcgggat gcttgcttcg ccccggatgg
    14221 ggaagctgta ccctttttcg tccacccaaa agagcgggct gtcattctcg taccaggtga
    14281 tggcagggat gcgttcgttg acggcgacgt tcaccacagc cgggaaggtc actgttactt
    14341 gcgcgtagtc gatatccggg aaggacgcgg caatttcctc acggatttgc tccggactta
    14401 ccctgatgat tggcctgcct tccaggtcga tcgtctcgag gatttcctgc gcggaaacgc
    14461 gcactgcgcc gctgagattg atgctggaaa cctggaaggt aggggaggaa ctgaccagat
    14521 agatgccgat gcctgccaga attgccagca gggcggaagc aatgcgccag gaaagcttga
    14581 gcgccggcat ggcggggagg cgaatttcgg cgccggattt atcggtgggc aggtagtact
    14641 tgcggcggcg ctgggtatag gctgccgaag ttggccgcgc catgttcagg tccgaccggc
    14701 ggactgtgac gcgcgctgaa ctttccgcgt tccgtttgga atacttttcc ggctgcggaa
    14761 ttggtttgcg gcgcgctggc tcaatgctgc ggcgcgctct cacctggtcc gctctggtca
    14821 aattctcgta acggggtgcc atctttgttt cctaatcctc atacctgcgt acggtctgat
    14881 cctgatccgc cttacgctcc aaccccaacg tgatcagacg gtccaccagc tgcggatact
    14941 ccagtccgga ggctttccaa agcttcggat acatgctgat actggtaaag cccggcatgg
    15001 tattgatttc gctcaggaag agttcgccag tgtgcttgtc catcaaaaaa tccacgcgcg
    15061 aaagccccgc gccgtcgact gcccggtagg cgcgcaaggc gtattcctgg attttttggg
    15121 tcatttcgac gtcaaagggc gcgggaatga tcgtctcggg gtcgccgctc aggtatttgt
    15181 ccttgtaggt gtaaaaaaca tcttcgggga cgatttcacc ggggacggac accagcgggt
    15241 aatcgttccc aagcacgctg atttcaattt cgcgcgcgtc aataccgcgt tccactaata
    15301 tgcgccggtc aaagcgcgca gctttcagca gcgccgcgcg caattcctcg cggctgcctg
    15361 ccttgctgat gcccaccgag gagccgaggt tggcgggctt gatgaagagg ggataggccg
    15421 aaaccgcttc tgcaagcgcg ataacctctt cgaggtaatc cgcgatttga ctgcggctga
    15481 acacctggta gggcagcaca ggaatgcccg cgttttgcag caggtccttg cagactgcct
    15541 tatccatgca ggccgccgaa gccagcacgc ctgcgccgac gtacgcgacg ttcagaatct
    15601 caaataaacc ctgaatagtg ccgtcctccc cgaaggtgcc gtgtagaacc gggaaaatga
    15661 catccaattc cgcaatcagt ttcagctttt cgtattcgcg ctgatacagc ccgacttttc
    15721 cgtccgtgct cagcaggctg acctcctgaa ggctgctggt atcgccattt tcgaaggcgc
    15781 tcagggcgtt ctcgccgctc agccagacgc cgtccagggt gatgccgatt tcgaacacat
    15841 tgtacttatc caggtccagc accgagcgga ccgaacggga ggacctgagc gatacggcat
    15901 gttcccctga gcggccgcca aataccaaag caagattatt cttttgctgg ttcatcaaaa
    15961 tcacctatca gttcaatttc cagttccaac tgcacgccaa atttcttttt cacggttttt
    16021 tgcgccgtac ggatcagctt gagatattcc tgcgcgctgg aattgccgtc atttactata
    16081 aaattcgcgt gcaagccgct gaactgggcg tgaccgtccg tcatgccttt cagacccgcc
    16141 gcttcaatca gcctgccggc ataatctccc ggaggattct taaaggtcga gccggtcgac
    16201 gcgccggtgg gttggttcgc ctggcgcttt tcggacattt ccagcagggc cttccaggca
    16261 gttgcgcgtt cgctgcgttc ggctttcagc ctcgcggaaa ggattagcag gtctttgggc
    16321 tcccgcttca ggatgctgct gcggtaggag aacatcagtt cttccgctgt cagggtcatt
    16381 tccccttcgc ctcgcacaag cgcctggatg gagaccagcg acctggaaat atctgagccg
    16441 aacgcgcccg cattgccgta cactgcgccg ccgaccgaac ccggcacact gcctgcccac
    16501 tccagcccgg aaagcccgcg cagggcgctt tggcgggcga cattgctgag gttcgcgccg
    16561 ctttcggcat aaatgctggg cggttcctcc tgggtgttga tgcggatgtt gtggcagtgg
    16621 tttagcagca cgatgccctg ataaccgctg tcgcttacca aaatattgga gccgctgcca
    16681 atgagcttga aaggtacgtc caggctccat aaggcgttcg ccgcgtcccg cagctcggct
    16741 atggtgttga ccgagaccag gcccagggcg ggcccaccca cgcgggaggt ggtgtagttc
    16801 gccatgcgca cgttttcctg cagacgcgcg ccgaagaagt cctgaagttt tcgcatcggg
    16861 atgctcataa atcgacctcc ccgctccgcc tcaggatgct ttcacgcagg ctggcgcaga
    16921 tttgcggccc tttgccggcg gaaaggaaca cgaccacgtc attcccggtc agctgcctgc
    16981 tgagcgcctc cacaagctct gtgaagtccg ggatgtagtc tgctttctca gccggcaggg
    17041 cgtccacaat cggttgtggg ctaaagccgt cgtcaggctc gcgcgccgcg taaattttgg
    17101 tgatcatcac cttgtctgcc aggtccagag cttgagcgaa ttctttctgc aggctctcgg
    17161 tgcgggagaa tgtatgcggt tcccacaccg cccacagctt tttggtcggg taaagctcgc
    17221 gcgcggcacc gagcgtggcg cgcacctggg tgggatggtg accgtagtca ttaatcaaaa
    17281 cctcaccggc gctttcgagc acgatgtcga agcggcgttc ggtgccggca aagcttgcca
    17341 gcgcagaggc gctcccggcc gcgctggcgc ccagagcgtc cacgaccgcg agcgcggccg
    17401 cggcgttcgc cacgttgtgt ttgcccggca gtctcaaggt aaactgaccc aacacttgcg
    17461 cctggcgctg ggatttgctg cgccgcaggc tgaaatgtga agccctgccg tcccagacat
    17521 aatcctcaat gcgataatcg cagccttcat ggaaaccgta agtgagcagt tcgatatctg
    17581 tgcgcaggtc cgcctttagc aaactgcgta cgcccgggtc gtcagcgcat agaatgctct
    17641 tgccccccgg tacggtgcgg ttgatgaaat ccttaaacgc gtccagataa tcctgcgggg
    17701 tagggaaaca atccgggtgg tcgtattcga tgttggtcac gaccgaaagc gcgggcgtca
    17761 gccccaggaa catgcggtcg tactcgtccg cctcaatcac aaagtgcctg ccgatatccc
    17821 aacgggcgtt ggtagcgagt tctttgattt ccgcgccgac gatgaagctg gggtcgtagg
    17881 tcagggcgtc cagcatgcaa ataagcatgg cggtagtggt ggttttgccg tgtgaaccag
    17941 ccaccgcaat cgtttccttg ccccaggtga gctcggagag gaaggcttcg cgcttaatca
    18001 cggggatgcc ggcagcgcgt gccgcagcga cctccggatc gtcatctctg acagccgagg
    18061 agcgcacaat gaggtcagcc tgcagggcaa gccgcggatc atgaccaagc gcgacccggg
    18121 cgccgcgttt gctgaccatc tgaaaatagg gagacgcggc caggtcggag ccgctcacct
    18181 ggtagccctt ttcgaggagt accacggcga tacccgacat gcctgttccg ccaatcccaa
    18241 tcaggtgcac gcgcttcatc agacaaaagc tccaatcaga aggaaaaacg caatcacaac
    18301 catgtagtac aaaatcggta catgcatgta tgtcgggaag gcggactcga ggatttttgc
    18361 ggcactctgt cccagttcag tcgccagatc cggggcagta atcgccttga acggatagcc
    18421 agcatccgcc atatagaacc acagcgagct aaccaggatg tatccgttga ttccacccat
    18481 cagccccccg gcgagcatat cccggaaggg tccgcgttgg aagcgtacat tctcggcaaa
    18541 gcggcgcagg ttgggggtct gataaccggc aagggcgcaa acgacgatca cgatggaatt
    18601 aatccagaat cgtttggaac cgctggcatc tccaaaaatg cggggaatga ccacttcgat
    18661 ggtgaagagc gccagaatag aggacgcgca caccacgatt tctttgccga aaccgcgcat
    18721 cgcgcctatc agaacgaata aggcgaccag tacatagaag aggacactca ggctgatcat
    18781 ctttggggct cctgtttcat gctccagcca tctccaataa cagttccgaa atggccaggg
    18841 cggcgtccgg gttggacagc gcgcgcatcg cggcgctcat ttcagccaga cgctgcgcgt
    18901 ctgtcattag gtccttaatc gtggcaagca ggttggcctt caggtacctg tcttccatca
    18961 gcacagccgc gccgcgctcc accagatagt ctgcgttgac tttctggtat ttccaggcgt
    19021 aagggtaagg aaccagaaca gccggcagtc cgaaataggg atactcgccc aaggtggaag
    19081 ctccggcgcg cgagacaacc agatccgcgc aagccatggc ggcgccgacc tcgtgcaggt
    19141 aggggaaggc ctgatagcgg gggccgagct ggtgggtgtg cttctggatt tcatcccagt
    19201 ccaggtgccc ggtcaggtga attacctgat actcgcgggt tagctcagca gcgatttcca
    19261 tcactgcctg gttgatggag cgcgcgccgc tgctgcctcc caaaaacagg atggtctttc
    19321 gctgggggtc aaagccaaag tacgcctgcg cctctgcttt ggtccatttc ttaaggtctt
    19381 ctcttaacgg ataacctgtc accgtgattt tctcctgatg tttaaaatac ctgcgcgaat
    19441 cttccacgct gaccgcgatg cggctggcga agcgtgccag taccttgagc gcgagccctg
    19501 gttcgatatc cggaacaaag aggactgagc gccgccggag gccggccact gccattggga
    19561 tggccacgta accgccggta aagaacagca cgtccggatt gaactgcttg agcagccggc
    19621 gggagctgtg gtaacctctc gcaaggcgaa ggaggttgcc gggcagcttc ttgagcgata
    19681 cgccgtgtac acccgcggca ggaacggctt tgaaaggcag gccggcgcgt tcgaccagcg
    19741 ccttttccat gccgccttca ctgccgaccc agagcagctc gtgggcatca ttcctgaggg
    19801 tttccgaaac ggttagcgcc ggatacactc cgccgccggt cccgcctgcg caaatcaaca
    19861 atcgcactgg tctggctcct ttccgcagat tcttttgcca caccctgcct ggcaacattc
    19921 attacaatcc caagggaact catcgtcgcg agcatgctcg aaccgcccgc gctgatgagg
    19981 ggcagggcat ttcccgtgaa cgggaacaag cccacaatca cgctgacatt catcaatgcc
    20041 tctagggcaa tccaactgat aagcccgaag ctgatcagcg aaccaagctg gtcgggagcg
    20101 cgtcgggcga tgaccagccc acgccaaagc agcagcacat acaacgccac caccaggaat
    20161 gcgccgacca ggccagtctc ctcggcaatc accgcgaaga tactgtcggt gtggggcagg
    20221 ggcaggccgg tgaacttggt gtctgcttcc ccgatgccga caccgaacaa gccgcctttg
    20281 atgatggcct cgtagatgcg ctggacgtgg tacgagctgc cggtcaggtc tttaagaccg
    20341 ctcaggtagt cgctgatacg cgcttgtccg gtgcgggaaa tcgaaaccac taccgcgccg
    20401 ataccgacaa caaagaccac gaaaatcaat aactttttca ggtcgccgcc ggccaggaag
    20461 aagagcagga ttccgagcag caccacggtg gcagccgcgc tcaaatccgg ctggaggaca
    20521 atcatgccgg cggtgaggcc gacgataaag agcagcggca gcagcccgtc cccgagcagg
    20581 ttcatcgtat cgcggtgcga ggacagccag aaggaaaggt aaaggataat gccgagtttg
    20641 gcgaattccg agggttggaa cgagccttcg aacagcgccc ggctggaatt gaagcgaatg
    20701 tcgttgcgga taagcactgc cagcagcagc accaggacca caccgagcgc gatgagcacg
    20761 aaaggccggt aaaagtgata atcgaggaaa ctcaggccga ccgccaacgc gattccgagc
    20821 aggacccaca tgatttggcg ggtaaagatg taggtcggtt ctttgcccat gcgcagcgaa
    20881 gcatccaggc tggtggaata taccatcagc agaccgataa ccagcaagct ggcgacgatc
    20941 agcagcagcg gaacgtcgaa cggcagtttg gatgcgctgt ttctgcgcgc agccggtttc
    21001 acgaattcgt tggtcacagc gcgttcaccc attgtctgaa gcactctcct cgttcctcaa
    21061 agtctttgaa ggcgtcgtaa ctggtaccgc ctggcgaaag caggacggta tcgccttccc
    21121 gcgcgagctc ggcggctttc tgcaccgcgt cctgcaggct cgcgcagcgc gtgatggact
    21181 gcaaggtctc gccggctttc acttccccca gcgcctggct gatgagcccg gcggcttcgc
    21241 cgaacaggac ggcatgccgc acgcggctgt gcacgtgttc cgccagcttt tcccagggca
    21301 ggtccttatc ccggccgccg agcagcagta cgagcggacc ttcgaaggac tgcagcgctg
    21361 ccagcgtgcg ttccggcgcg gtggcaatcg agtcgttgta ccagtctgct ccacgcacat
    21421 tccgtacaaa ttccagccgg tgcggcacac cgctgaattc ttctacagcg ctctgaatgg
    21481 ccggcagcgc cagcgaagcg cccgcggcgg ccgcgcaggc tgccagcacg ttgctgatat
    21541 tatgctgacc gcgtagttgg atccagtcga gagggaacat tttcagctgc tggcggtcaa
    21601 tctgcaggta gatggaatcc ttatggatat acgttccgtt ctgattgcct tccgggcggt
    21661 tgaaaccgaa gctgatcagg tcgcttttca actgcgcggc aagcgcccag gagccggcgt
    21721 cgtcgcggtt caagacagcc acatcaccct ggtgctgata attcaggatg cgggccttag
    21781 ctgcgctgta agcctgcatc gttacatgcc gatccaggtg gttcggggtg atattgagga
    21841 ctaccgccac ctgcggtgat acagtcatga gctcgagttg gaagctggaa agttccagga
    21901 tgaccaggtc atccgcgtgc atttccccga cctgcccgat gagggggttg ccgatgttcc
    21961 cgccgaccca ggcacggtgc gggctttgct tcatttcgaa gtatttactc gccatcaaac
    22021 ccaacagggc ggtcgtggtg gtctttccgg ccgaaccagt gatgccgacg accggcgcgg
    22081 ggcaggcttc caggaaaatc tgggaatcgt tcgagagctt aatgccccgg cggacagttt
    22141 cctgcaggaa aggaatattc agcggcacgc cgcccgagac gcagaccagg tccgcgcctt
    22201 cgagcagttc cagcgggtgg ctgcccaggc agagctccac gccggcatcc ttaagcgtca
    22261 gcacgtcagc ggagagcgcc tcgggtccgt gcaaatccgt caggacaacc cgcgcgcctt
    22321 tgcctgccag gaagcgcgaa agcgccgtgc cctggcgggc agctccgaga acgatgactt
    22381 ttttgtcagc ccagtttttc ataattatcc caaaaaggtc agagcaaagc caagcatcgc
    22441 tccgatgaag ccaatcaacc aaaatcgaat cacaatttgg ttctcctgcc aacccagcat
    22501 ctcaaaatgg tggtggatgg gggctttttt gaacacccgt ttgccgtgcg tcagcttgaa
    22561 gtacccaacc tgaataatga cgctgagcag ctcggacacc ggaatgatgg cgatgagcgg
    22621 gaggaaaacc caacgaccgg tcatcagcgc tacgactgcc agcatcgcgc ccaaagcttg
    22681 cgaacccgtg tcacccatca tcagttccgc cggcttggca ttgaaccaca aaaagcccgc
    22741 cagcgctccg acgatgcaga agcagaagcg cgccaggtaa acctgaccct gaaaaattgc
    22801 aatgatgccg tagaccccga acatggtcat gcagatcatg ctggccaggc cgtcgatgcc
    22861 gtcggtgaag ttcacggcgt ttgccatggc gacgataata aacgccgcaa tcggcagata
    22921 ccaccaaccc agattcagag gagcctcgga ggcaggccag aacacgttgg ggaccctcag
    22981 gatttctttc aggctgtagg cgattgcaat cgccacgacc gcctgaagcg tgaacttggt
    23041 gcgcgcgctc atgcccagcc cgcggcgcgg tccgcgaata ccttcccagt catccaccgc
    23101 gccaatcagc gcgaacagcc ataaggtcgc cagcgggacg aaaatcgaag agccgaacag
    23161 gcgcgacccg aacagcgaga ccgcgttcag gatgacggtc aggatggtga ttggaatcag
    23221 gaacatgaag ccgcccatgg tgggggtgtt catcttcgcc aggtgcgact ccggcacatc
    23281 catactgata atcttgccaa ttttcaggag ttttaacagc cggataaacg gagaccccca
    23341 aatcaccgtg agcaaaaagc tgaaaccgct gatggctatt gccagggaag ttccgcccat
    23401 caggaaatta cctccaaact tgccacgatc aggtccatgc gcatcgcgtg cgaacccttg
    23461 accagggcga tatctttttc tttcagtgtt tcccgcaagt accgggcggc gctgtttgcg
    23521 tcggcaaacc aatgaatatg attcggcgcg aaaccgcttt ccaacgcggc tgcttgcgtc
    23581 tgcaggctgc gctgacctac caggacgatt tcgtccgcga cctgagcggc gcggatgccc
    23641 acccgctggt gcccgtcttc ctcgtactgg ccgagttcca gcatgtcgcc gagcacggca
    23701 actttccgac cggtgaggtc ttcgagcaag ttcagcgccg cgagggttga atccggggaa
    23761 gcgttatacg tgtcgtctat cagcagcgcg ccggacgcgg ttttggtggt cgcgatgcgc
    23821 agctggaggt tgctggtttt cagggcgtaa aatatccaat cccaggaaaa gccgaggctg
    23881 agcgcggcag cggcggagcg cagcatcgtg tagaccgaat gccggccaat cagcggcgcg
    23941 gtcacgtaaa agtcctcacc ctggtagtgg atgcggcagc ggatgccttc caaacccatg
    24001 ctttcaacct gatccgccca caggtccgcc tgcgggcata aaccgtaggt aaacacccgc
    24061 gcggaggttt tggcagccat ggctaatacg cgttcgtcgt cgtaattcag aatggcgacg
    24121 ccttcggggg cgggtgggag cgcttgcacc aactccgatt tgccctccgc gatggcttct
    24181 atggaaccgg cgcgttcagc gtgtactgtg ccgatattcg tcacaatccc gatttggggg
    24241 cgggcaatct cgcaaagcag tttgatttct cccgggacgt aaaaacccat ttctaacacc
    24301 gcgacctcgt gctccgcgtt gaggctgagc agggaaaggg gcatgccgat ttcgttgttc
    24361 atgttgcccg ggtttttgag cgttaccaga cgcttggaaa gcacgttggc ggtcaattct
    24421 ttggtggagg acttgcctac gctgccggtg atcccgatga ccttgatatc aaactgggcg
    24481 cgccagtacg ccgccagttt ttgcagcgcg gcaacgacgt tgtccgtcag caggctgaaa
    24541 ggcggtcgct ccggaaattg aagctccgcc gcgttcagtt caacgatggg atatgcgctc
    24601 tcaatcgggt gctccagaat cgccaggctg gcgcccctgg caaaggcatc gtccacgtac
    24661 aggtggccgt cggtacgttc cccgcgcagc gcgaagaaga gactgccctc gctgactgcg
    24721 cgcgagtcaa taaacgcctc cgggagcttg aaatcaagct ccccgacagc gcgcccgagc
    24781 agggctttga gcacgtgctg aaaggtcagc attttatttg tcctccgtgg acgcctgcag
    24841 ccggattgga tcaggcggga tatccatcag cacaaccagg ttttgcacaa ctttcgagaa
    24901 taccggcgcc gcgacttccg agccccagat gctgatggtt ggtttttcca gccagacgta
    24961 aaccagaaat ttcggatctt cagccggacc ccaacccaca aaggaagtgt tggtgacccc
    25021 gctggtatag ccgaaaggcg tggggatttc cgcggtaccg gtcttgccgg cgaccgtata
    25081 tccctccacc agcgcgacgg atgattcttc ctccagggag tccaccaaca tgcgggtgag
    25141 cgtttccgcg gtctcggctt tgataggatt cccaacgatg accggattaa cgtcgtattg
    25201 gtgtccgtcc atgatgatgg ccttaacgat gtgcggcgcc atcatgcgac cttcattcgc
    25261 caccgcgccg atggccatgg ccatttgaat gggcgtcacg gaaatgccct gcccaaacga
    25321 gttggttgcc aggtcccctt cataccactg gttgtcaccg ggcagtttga gaggccagtg
    25381 cgattccccg cccaggtcga tgcctgtgtt gcggtcgaag ccgaagtttt tcagatacgt
    25441 atagaactgt tccgcgccga gctgggtggc aatccacgac aaacatacgt tcaatgagtg
    25501 ctgcaaacag ccggtcatgt cctggtcacc ccacgcgccg tagttccagt tggtgatggt
    25561 agtgccgccg accatgatgg accccgtgtc ggtgaagact gtgtccggtt ttaccgcgcc
    25621 agcatccagg gcggaagcca tagtgataac tttgaagacc gagcccggct cgtaggtctc
    25681 gctaatcggg cggttgaacg gcgtgctgcc ggggaagacg tctccctggg tccagtaata
    25741 gttgggatcc attctggggg tggtcgccat ggcgatgatg ctgccgtctt ccgggttgta
    25801 caccacgatg gtgccggatt ctgctccgga ccaatctatt gcttcggcaa gctgctgctc
    25861 caccatggcc tgaatttccc ggtcaaaggt caatacgagg ctggcgcccg ggggaatgtc
    25921 gtcgaccgag gtaatctttt gcgggtcgaa gggggtgtat tccgttttgg gcgttccggc
    25981 aagcaggtca ttgtaagctt cttccacgcc gtaatatccg cggccttcca cgcggtccag
    26041 gaaggcataa aaaccgagca ggttagcgcc catatcgcct tccgggtaac tgcgcttgtt
    26101 gtaaggggtc cagattaagc cctcgaggct gggtcttgtt tcgttcttgc cgatatcgcg
    26161 gtttgtgtaa tcttcttcga tcaattccag ttgtgcgatt ttttccttat cgacgaaaga
    26221 atcgagcacg atgtaaagtt tttcgtcctc aatcggttcc gttttggcat aaacgagggt
    26281 gctgctgtag tccaggccaa gcacgctgct cgccaccgag gcaatggtct ccggctgggc
    26341 aatgctgcgc aagtccagcc cgacttcgta gacggtttcg ttgccagcca agagccgtcc
    26401 ctgcgcgtcg taaatgctgc cgcgttccgg gtagatggtc tgggtcacgc caaccagctt
    26461 tttgctgttt tcgagtatcg tctgcgccgc tggaatattc tggatgctta ccatgcgcgc
    26521 gccaacgctg ccggcgacca gggcgaagac cagcaaaatc aagatgtagc gcgtccagga
    26581 tggattattc acggctgcac ctccggagtg ttttccgccg gacccggcag ctgcgcaagt
    26641 atatcaccca accagtccca gagggaagcg gtgaattccg gcaggatcga ggggctgggc
    26701 agggcgttcg cgctgggggc gggggccagg gtaaagcttt ccgcgctgta ccccggcact
    26761 tccaggtaga ccgcttgcgc cgggtcgcgt tgaaccatgt ctaaggcttt ggctctctcg
    26821 ctcatcacac gcgcggagga tgcatatgcc agttgggaag cgaggtcgtt aatctgcagt
    26881 tccaaggcgc tcacctggta ttccagcttc tggattctgc gcccggttac agcggctttt
    26941 ccgctgagag agagatacac gccggcaatg gctgcgatgc tcacgaggat cagcagaaaa
    27001 atgccaatcc actggagctg catgcgccag ggggcttgtc gataggcttg gatgatgttc
    27061 ttggttttca tattcgtaag gctgcagatt ctatgccatt ttgggtaact tttgtgccac
    27121 tctcagtttt gcgctgcgcg cgcgcgggtt ttcttttttt tcctgttctc cggcttccac
    27181 cggatggtgg gtgacgatgg tgatgctcgc acggtgtccg caggtgcaga caggctgctc
    27241 aggcgggcaa atgcagtccc ggctttccct ttggaaggtc tgcttgatga tgcggtcttc
    27301 caacgaatgg aaagcaatga ctgcgatccg ggcgttcggg tgtaaaagtg cgattaactg
    27361 cggaatcgct tccgcgataa ctgccagctc gtcgtttact gcgatccgca gggcttgaaa
    27421 agtacgggtg gcagggtcct gggcttcatt cgtcctgcct aaagccgagc gcaccagggc
    27481 cgccagttcc gtcgtagttc tcagaggtct tgcgcgtaca atcgcccggg cgatgcggcg
    27541 ggagtagcgc tcctcgccgt acttccagag gatatccgcc agttcctttt cctccaggcg
    27601 gttcaccagg tcggctgcgc ttggtccgct gcccgggtcg aagcgcatgt ccagcggtcc
    27661 ttcctgccgg aaggaaaagc cgcggtcggc ttcgtccagc tgcatggagg acacgcccag
    27721 atcgaggagg atgccgtcca ccgcttccca acccaactcc tggaggtaat cccggctgtg
    27781 caggtagctg ccctgccgga cgattgcgcg ttcgccatag ggttcaaggc gggctttcgt
    27841 cagcgccact gcgcgggggt ccaggtcaat ggcgagcagt ttcccagcgg gcgcgctggc
    27901 ttccaggatg ccgctggaat gcccgccggc gccagccgtg cagtccaggt agcgcccggg
    27961 gctgtggggt gcaagcaggt ttagaatttc ttggtaaagt acaggacggt ggtggggttg
    28021 atcccccatt cccgcagaca ttaaccacct ctggtcgaga tgttataagc tgcccaacgc
    28081 ccgctgttcg cttcggcgcc gccgtttttc tggttttccg cctgccaatg ttccatcgac
    28141 catatttcca cgttgtcgcc gttgccgacg agtttgcaag ccgtcgtgat ttgcgctgcg
    28201 tcacgcagaa aggcaggcag caggattcgt ccggcgctgt catagctgag ttcaacagta
    28261 ttggagaaga acatgcggcg cagggcgcgg gattcgggat ccgttacgga aagctcgtta
    28321 atattctccg ccatgcgttc gtacacgtcc cgtggatatg cgataagatt ggaatcaaag
    28381 cctttggtaa cgaagaggcc ttccggggct tgttcccgga aattggaggg gacggtgagt
    28441 ctccctttcg cgtcaatgtt atgctcaaac tgacctaaga acataagttc taattccttt
    28501 ttaatcgcga aacgctgggc aagttccagc gctccaaatg acacttcgtg acactttatg
    28561 acattatttc ccacattata gatgtttcta tgggaaaatg caagggtttt ggcacacaaa
    28621 ttgcagatat tttaagattt cacgacccag gaatggaatc cggcgcgtga tacccacttc
    28681 ccgctgattc agcgcctgaa ttttgctcca ggatcaaaat ccagcccctg gcagccgcgt
    28741 tttttgggtc tctttttgat ttataatgag gactaatgac accacccggc attcttaatg
    28801 accgctatca gctgatcgag tcccttggga cagggggcat ggcgcttgtg tacaaagcca
    28861 aggacctgat gctcgagcgc tacgtggcag tgaaggtttt gcgagaggat ttctcggtgg
    28921 acgccgcatt ccgggaacgc ttccgccagg aggccaaagc agcggctaac ctttcccatc
    28981 ctaacatcgt gacggtacac gacttcggcc tggaccagcg ccatctcttc atcgtgatgg
    29041 agtttgtacc cggaaccgac ctgaaaaccc gtatccgcga aaaaggccgc tttgaagtgc
    29101 aggaagcgct ggacctgatg gtccaggctt gccagggggt tggctacgcc caccgcgccg
    29161 gcctggtcca ctgtgatatt aagccgcata acatgctcgt cagcccggac ggacgtctca
    29221 aggtgaccga ttttggcatc gcgcgggcgc tggcgaccat ctctcctgaa gagcgcagcg
    29281 acgtggtgtg gggctcgccg ctttacttct ccccggaaca ggctgccggc ttgccgcctt
    29341 ccccggcttc ggatgtgtac tcgctgggcg tcatcctgta cgagatgctg accggtcagc
    29401 tcccgttcaa ttccgcggat gcagcggaac tggcgcggat gcaccgcgaa gtgcagcctg
    29461 tgcccccgcg cgtgctcaat cccggcatcc ccaaagtgct ggaagacatt atcctgaagg
    29521 ttctttccaa ggaaccttcc gcgcgctatc gcaccgccga ccagttaggg cggctgctgg
    29581 tcgggctgca agcgatgaag agcggcagcg cgcagccgca ggtggtgact accccaggcg
    29641 catccgccgt gccagcgcag acagccccgg cgccagtgcc tgcgccaccg gacactcccg
    29701 tttaccaaac ctcaccggaa gcgctcgaaa cggacacggc tgaaaagaaa agcccggatt
    29761 ggggcacctt gctgctgtgg ctgattgctg gcgccgcaat gttcggtttg attcccttct
    29821 ggctgtacgt ttacctgacc ctgagaccct gaggttttgc atgctcgcga ccatttcccc
    29881 ttccatcctt tccgccaacc tggcaaacct catccaggat ttgcgcgagt gcgagagcgc
    29941 cggagcagac aaaatccaca tcgacgtgat ggacgggcgt tttgtcccga atatttcgtt
    30001 tggtccggtg atcgtcaggg cctgcaagca agcaacctcg ctgcccctgg acgtgcacct
    30061 gatgattgtg gagcccgaaa agcatctgga cgcctttgtg gaagcagggg cggatgctat
    30121 cacggtgcat tacgaaacct gcccgcacat ccaccgcacc ttacagcgca tccgcgattt
    30181 aggcgccgcg ccgggtgtca ccttcaatcc ggccacgccg gtggacggct tgcgctatct
    30241 ggcaggcatg ttcgacctcg tcctgattat gtcggtcaac cccggatttg gcgggcaaaa
    30301 gttcattccc tcggcgctta cgaaagtacg cgatacgcgc caactgctgc gggagattgg
    30361 ctcggacgcg gtcatccagg tggacggcgg cattgacgcg agcacgatcg cggcagtgta
    30421 cgaagcaggc ggccgggatt ttgtggcggg cacggctgtg ttcgggcatc ccggcggcat
    30481 cagcgcgggg attgccagct tacggcaggc tctggttaaa tgaaaaaagc cgcgtgagcg
    30541 actttttctc aggtcttgtg ttaattaatt gtatgctttg gtcagggtct taatacagcg
    30601 cgcgcagaga actttgcgaa ctttcttgcc gttttcgaac actgtgatcc gctgaaggtt
    30661 tgggcggaac tcgcgccggg ttgcgacaag agagtggctg cgattatgac cgaaactggt
    30721 tgatttacca cagtgattgc actttgccat ctcgatttcc tttcatacag tataatgtcg
    30781 aggttgggaa ggattgaccg acccaagcaa gcgtttataa tatcacacat tatcataaga
    30841 atcaaggcgc gaatagagaa gagagacaac taaaaatgac aagtcagcaa aacccattag
    30901 gacgaattta cattct
//
LOCUS       DAOQAD010000013        27925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_1689_length_27925_cov_6.636383, whole genome shotgun sequence.
ACCESSION   DAOQAD010000013 DAOQAD010000000
VERSION     DAOQAD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 27925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27925
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1689_length_27925_cov_6.636383"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /gene="rpsI"
                     /locus_tag="PKL60_02150"
     CDS             complement(<1..382)
                     /gene="rpsI"
                     /locus_tag="PKL60_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNW94969.1"
                     /translation="MAQYFEAVGRRKESTARVRITAGSGVFTVNEKPLDAYFPRTGDA
                     PMILKALEAAGQNPSLTDVTVKVEGGGVSGQRDAVKLGLARALVSMDEGARVGLREKG
                     LLTRDARVKERKKPGLKRARKAPTY"
     gene            complement(396..824)
                     /gene="rplM"
                     /locus_tag="PKL60_02155"
     CDS             complement(396..824)
                     /gene="rplM"
                     /locus_tag="PKL60_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNW94970.1"
                     /translation="MEKTYVPKGQTVGEWYLVDAAGETLGRLATRIAVLLTGKHRADY
                     TPGVVLNDHVVVINAAQITFNQTRAEEKKYYRHSLYPGGLKSVTIKRQMEVKPEAVIR
                     AAVRGMLPKNRLTVRYLANLKIYAGPEHPHEAQDPKPISE"
     gene            complement(877..1626)
                     /gene="truA"
                     /locus_tag="PKL60_02160"
     CDS             complement(877..1626)
                     /gene="truA"
                     /locus_tag="PKL60_02160"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNW94971.1"
                     /translation="MAHYKLILAYEGSAYHGFQRQQNRHSVQAEFESALRKLGWQGRA
                     VMPAGRTDSGVHARGQVISFSLDWKHGTETLVTALNAHLPADIAVQAAEEVPLAFHPR
                     YDALSRTYRYQVYAQRARNPLVDRFSWRLNCMPDLEKMNRAAGLLVGEHDFRAFGKAL
                     KEDATTMRKVFSAEWQGGSQEAAFDICGNAFLYHMVRRIVFIVVQIGLGNLPEEIVSR
                     GLESGETGIVSLAPARGLTLWEVAYPEDGRK"
     gene            complement(1674..2057)
                     /gene="rplQ"
                     /locus_tag="PKL60_02165"
     CDS             complement(1674..2057)
                     /gene="rplQ"
                     /locus_tag="PKL60_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNW94972.1"
                     /translation="MRHQVKGYRLGRSGGHRTAMRKTMIMQLFEHEKIQTTLAKAKSI
                     QPDAEKMITLARNSLSASEVEKLNARRQISAELGNASADTVKKLFDDIAPRFKTRPGG
                     YTRLLKLGPRLGDNAEMVLLELVSE"
     gene            complement(2064..3008)
                     /locus_tag="PKL60_02170"
     CDS             complement(2064..3008)
                     /locus_tag="PKL60_02170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNW94973.1"
                     /translation="MVTPKVEGKKQSRETGQFTISPLERGFGITLGNAIRRVLLSSLE
                     GAAITAVRITDVMHEFSTIPGVREDVIQVMLNLKQIRMKLYDVDTAHLQLDVRGEGTY
                     TAADIICPPEVEIINPDLYLFSITDNAAHVEMEFTVERGRGYMPAGERAERLPIGVLP
                     VDAIFSPVRRVNWSVTNARVGHNTNYDKLNLEIHTDGTVPPEVALQSAVDLLIRQFSF
                     ISGTSGDMLESEVEVEEADHMGGIEAAETPIEALDLTVRVYNALKRTGVSSVRDVREM
                     LAKGENHMLSIRNFGEKSLVELRAKMIEKGYLKEEKAE"
     gene            complement(3078..3716)
                     /gene="rpsD"
                     /locus_tag="PKL60_02175"
     CDS             complement(3078..3716)
                     /gene="rpsD"
                     /locus_tag="PKL60_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNW94974.1"
                     /translation="MARYTDPVCRLCRREGEKLFLKGERCFSPKCAFEKRGYPPGMHG
                     KMSSARPGRESDYAKQLRAKQKARRVYGIYERQFRRFYGQALKMRGLTGNNLLQILET
                     RLSNVVYRLGFAASRPQARILVSHGHFLVNGERADIPSYGVSVDDVISVREKSRDTIY
                     FKNLREEAEGRTVPGWLARDMTNLSGRMLRLPERVEIDGNLNEQLIVEYYSR"
     gene            complement(3740..4144)
                     /gene="rpsK"
                     /locus_tag="PKL60_02180"
     CDS             complement(3740..4144)
                     /gene="rpsK"
                     /locus_tag="PKL60_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNW94975.1"
                     /translation="MAKQARTARRTTSARKVKRQVSTAQVHVYASFNNTIVTVTDLQG
                     NTLCWGSSGSAGFKGSRKSTPFAARMATEQTIKTAQTMGVQEVELFVKGPGPGREAAI
                     RAVQSLGIKVRLISDLTPVPHNGCRPPKRRRV"
     gene            complement(4249..4638)
                     /gene="rpsM"
                     /locus_tag="PKL60_02185"
     CDS             complement(4249..4638)
                     /gene="rpsM"
                     /locus_tag="PKL60_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNW94976.1"
                     /translation="MARIEGVDLPRNKRIQIGLRYIYGIGPKTADEILAATGVDPDIR
                     VKDLPENEVSKLRDYISQNLKVEGDLRREVQMNIKRLVEIGCYRGLRHRRNLPTRGQR
                     TRTNARTRKGPKKTVAGRGRRRGATKK"
     gene            complement(4650..4763)
                     /gene="rpmJ"
                     /locus_tag="PKL60_02190"
     CDS             complement(4650..4763)
                     /gene="rpmJ"
                     /locus_tag="PKL60_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNW94977.1"
                     /translation="MKVSASIKKRCGKCKIVKRDGRLYVICTNPRHKQRQG"
     gene            complement(4837..5601)
                     /gene="map"
                     /locus_tag="PKL60_02195"
     CDS             complement(4837..5601)
                     /gene="map"
                     /locus_tag="PKL60_02195"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNW94978.1"
                     /translation="MEGSLNIKSAAEIAIMREAGRINAEALEAAKAAIAPGVTTAELN
                     EVVEAVQRRYGVYSPFKNYPGPYPFPASICASVNEEMVHGIPDKRKLKEGDIISIDCG
                     TVYQGFVGDSAFTVGVGEISSQAQRLIEVTKQALDAGIAQMVPGNHLGDIGAAVESLV
                     LKHGFHVTHLYTGHGVGREMHESPLVPNYGQPGQGMLLREGMTLAIEPMVLVGTRYTR
                     ELKNQWTVISRDRSLTAHQEHTVAITANGPEILTRL"
     gene            complement(5601..6263)
                     /locus_tag="PKL60_02200"
     CDS             complement(5601..6263)
                     /locus_tag="PKL60_02200"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNW94979.1"
                     /translation="MNEKATYIVMLGPPGAGKGTQAKVIAEKLGLVHVSSGDLFRENL
                     SRQTDLGKLAQTYMTKGELVPDDVTIAMVKERLSRPDCASGAVLDGFPRTPAQAEALN
                     GILAEMNGKIDLVPLINVPDQVLVERLSGRWMSPSGRVYHALYNPPKVQWLDDVDGSP
                     LYQRDDDKPETVQHRIDVYKAQTAPLIDFFQAAGVLVEVDGTQDIDRVTEDILNAIGS
                     GC"
     gene            complement(6271..7602)
                     /gene="secY"
                     /locus_tag="PKL60_02205"
     CDS             complement(6271..7602)
                     /gene="secY"
                     /locus_tag="PKL60_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNW94980.1"
                     /translation="MKRSSWRFLWKSEDIRNKLLITLLLLAIYRLAANIPVPGIDRAT
                     VAALTTLKGTQGNLINLLDMLSGGAVSNFSILAMGVYPYITAQIILQLLQPVIPALEK
                     KMQDDPREGRIWMEKWTVILTIPMALLSAFGQINIFNQMLGSSIITNWGFTAGKLMPT
                     LTAIFAMVAGTMLGVWIGQLISEFGLRNQGLSLIIFSGIVSRLPQSLLKMISSKESWW
                     MIFIVVIILIITIFAIVYVQQGRRNVPVMFPGRRIGNRMSMPVRSNLPLMVNMAGMIP
                     IIFAQSFLTFPAILASFFLNSKTAWLAKSANWVTGAFGAEAAWYPVMFFVLVVLFTYF
                     YTDVMFTQQNYGDNLKKQGAQIPGVVRGKPTQDYLTRVQRRITLPGAFFLGFVAVMPY
                     LFSAVVPAGSSATLLLSSSGLLIVVGTVRETVQLIETELRMHGYDDRLINS"
     gene            complement(7623..8081)
                     /gene="rplO"
                     /locus_tag="PKL60_02210"
     CDS             complement(7623..8081)
                     /gene="rplO"
                     /locus_tag="PKL60_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNW94981.1"
                     /translation="MQLHDLKPNEGSKKLRKRVGRGHGSGSGKTSGRGIKGQNSRNGG
                     GVRLYHQGGNLPLFRKLPFLRGEGFSPLNRVRYAEVNLDALHGFAAGAEVTPEALAEA
                     GLLKDKNKPIKLMGRGDVNVALRIKVHKVTDGARQKIEKAGGSVETIALA"
     gene            complement(8081..8293)
                     /gene="rpmD"
                     /locus_tag="PKL60_02215"
     CDS             complement(8081..8293)
                     /gene="rpmD"
                     /locus_tag="PKL60_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNW94982.1"
                     /translation="MAKQTKKSSRVRVTLKKSGTGYPVRQKATLKALGFHHVNQVVEH
                     EDSPALRGMLTKVSHLVEIETVNEEK"
     gene            complement(8286..8813)
                     /gene="rpsE"
                     /locus_tag="PKL60_02220"
     CDS             complement(8286..8813)
                     /gene="rpsE"
                     /locus_tag="PKL60_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNW94983.1"
                     /translation="MMEDYQNEETTLDERVIDIARVAKVVKGGRRFSFRVTLVVGDNN
                     GKVGLGIGKAGAVSDAMRKATEHARKNMHEVSLLGTTIAHEVTGTQSGAKVLLKPASP
                     GTGVIAAGGVRAVCEAAGIKDILTKSMGSNNMLNIVQATFDALGQLKSPQTVAAERGK
                     DVADVTPFWERRKHG"
     gene            complement(8823..9188)
                     /gene="rplR"
                     /locus_tag="PKL60_02225"
     CDS             complement(8823..9188)
                     /gene="rplR"
                     /locus_tag="PKL60_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNW94984.1"
                     /translation="MANKSRNAARLRRHTRVRKKVFGTPERPRLNVYRSITEIYAQVI
                     DDHSGTTLVSASTVDTLLRDAVKGKPKAEQAVLVGEEIARRALAKGIKSVVMDRGGYY
                     YTGRVKALADGARKGGLEF"
     gene            complement(9200..9748)
                     /gene="rplF"
                     /locus_tag="PKL60_02230"
     CDS             complement(9200..9748)
                     /gene="rplF"
                     /locus_tag="PKL60_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNW94985.1"
                     /translation="MSRIGRLPIAIPAGVEVKIEGNKVSVKGPLGQLEQEFSSEIGIK
                     LEDGHLIIERPNDEPRVRALHGTTRALLNNMVNGVSKGFERVLEVNGVGYRAELNGKD
                     LVLNVGYSHPVTYPAPEGITFEVETKTRLVKVKGRDRQQVGQVASNLREIRPPEHYKG
                     TGIRYQGEVVKLKAGKAGKTGK"
     gene            complement(9758..10171)
                     /gene="rpsH"
                     /locus_tag="PKL60_02235"
     CDS             complement(9758..10171)
                     /gene="rpsH"
                     /locus_tag="PKL60_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNW94986.1"
                     /translation="MTISDPIADMLTRIRNIAMAGQSLVVMPNSKIKEEVARILQEEG
                     YLESYEVVDGKANGSKVLRLKLKYIGERRHRRPIITGLQRVSTPGCRVYTSRDEIPWV
                     LSGIGIAILSTPKGIMTGQRARKVGVGGEILCKIW"
     gene            complement(10192..10374)
                     /locus_tag="PKL60_02240"
     CDS             complement(10192..10374)
                     /locus_tag="PKL60_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNW94987.1"
                     /translation="MAKKCMKYREMRREYPNQVVNRCRICGRPRGYMRKFGLCRICFR
                     ELALQGKIPGVKKSSW"
     gene            complement(10374..10919)
                     /gene="rplE"
                     /locus_tag="PKL60_02245"
     CDS             complement(10374..10919)
                     /gene="rplE"
                     /locus_tag="PKL60_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNW94988.1"
                     /translation="MNKLQEQYKNEIAPALFKELGLKNVMQTPKIVKVVVNIGVGEAL
                     DNPKALEAAVSDMTAITGQKPVVIAAKKAISNFKLREGRQIGVKVTLRGEKMWAFLDR
                     LINIALPRVRDFRGISADAFDGRGNYTLGLQEQLIFPEIQYDKIDKVRGMEVSIVTTA
                     ENDDHARQLLSKFGMPFRKGN"
     gene            complement(10937..11332)
                     /gene="rplX"
                     /locus_tag="PKL60_02250"
     CDS             complement(10937..11332)
                     /gene="rplX"
                     /locus_tag="PKL60_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNW94989.1"
                     /translation="MKIRKGDQVKVTRGDESDKGKTGEVIRVLPKEERVVVQGVNLVK
                     KHQKQTQSGSKTIPAGIREFEAPIHISNVELVKQGAEKADGKAEKPGRPTKTGARKAP
                     AKAVRKEAKPAARKRAADKPADDNSGSGK"
     gene            complement(11360..11731)
                     /gene="rplN"
                     /locus_tag="PKL60_02255"
     CDS             complement(11360..11731)
                     /gene="rplN"
                     /locus_tag="PKL60_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNW94990.1"
                     /translation="MIQQEARLTIADNTGAKELLVFHVVGGTRRHYAYVGDIVIGTVK
                     SASPQASVKKSEIVKAVIVRVSKEYRRPDGSSICFDDNAAVILDNDGVNPRGTRIFGP
                     VARELREKGFMKIVSLAPEVL"
     gene            complement(11728..12018)
                     /gene="rpsQ"
                     /locus_tag="PKL60_02260"
     CDS             complement(11728..12018)
                     /gene="rpsQ"
                     /locus_tag="PKL60_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNW94991.1"
                     /translation="MNKRRRLIGTVTSAKTAKTVSVEVSRTYQHPLYHKVVRGSKVYK
                     AHDELNCSVGDKVSIVECAPLSATKHWIVEKIIRVEVREAGELDDISEGEAA"
     gene            complement(12020..12223)
                     /gene="rpmC"
                     /locus_tag="PKL60_02265"
     CDS             complement(12020..12223)
                     /gene="rpmC"
                     /locus_tag="PKL60_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNW94992.1"
                     /translation="MNTKEIRELSTEEIKNKLMDSRQELMNMRFQMVTGQLTDTSRFK
                     VTRRQIARFETILAQRERKGKEG"
     gene            complement(12220..12645)
                     /gene="rplP"
                     /locus_tag="PKL60_02270"
     CDS             complement(12220..12645)
                     /gene="rplP"
                     /locus_tag="PKL60_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNW94993.1"
                     /translation="MLMPKRVKYRKQMRGRMKGKAQRGVEVSFGDYGLKSLECGYLTA
                     RQIEAARRAIVHELKRRGKVWIRVFPDKPYTKRAAETRMGKGKGSVDHWVAVIRPGRV
                     MFEVGGTDEESALKALQRARYKLGFRTKIVTKELVGEGS"
     gene            complement(12674..13351)
                     /gene="rpsC"
                     /locus_tag="PKL60_02275"
     CDS             complement(12674..13351)
                     /gene="rpsC"
                     /locus_tag="PKL60_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNW94994.1"
                     /translation="MGRKVHPIGFRLGINKPWLGRWYAEGSDYTEQLHQDLRIRELVL
                     KGAERAGVSGVEIERYPGKVKVSLHTAKPGVLIGKKGESVKILRQELEALTGKKVDLD
                     VKEIKNPDTDAYLVAQNIAGQLERRISYRRAMKRAIQQALRQGAGGIKVSVSGRLSGA
                     EMARRVTLREGRVPLQTLRADIDFARAEASTTYGQIGIKVWIYRGIVMGLAEEKIEAT
                     EGVYISE"
     gene            complement(13379..13720)
                     /gene="rplV"
                     /locus_tag="PKL60_02280"
     CDS             complement(13379..13720)
                     /gene="rplV"
                     /locus_tag="PKL60_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNW94995.1"
                     /translation="MAQEIKASLSNLSTSAQKARLVVDLVRGKKVVDALGILSLTPNK
                     AAEPVRKLLYSAMSNAEENFGVSREDLVVYKIFADEAPTRKWRSFGARGRFKPQLRRS
                     SHVTVVLREIE"
     gene            complement(13728..14000)
                     /gene="rpsS"
                     /locus_tag="PKL60_02285"
     CDS             complement(13728..14000)
                     /gene="rpsS"
                     /locus_tag="PKL60_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNW94996.1"
                     /translation="MSRSLKKGPFVDPKLLKRIEEMNAKGDKKVIRTWSRASTIFPQM
                     VGHTIAVHDGHRHVPIYVTDNMVGHRLGEFAPTRWFRGHVQEKSTK"
     gene            complement(14032..14871)
                     /gene="rplB"
                     /locus_tag="PKL60_02290"
     CDS             complement(14032..14871)
                     /gene="rplB"
                     /locus_tag="PKL60_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNW94997.1"
                     /translation="MAVKIYKPKTPGQRHRTSYTFEEITKDRPERSLIVSGKKTAGRN
                     SLGRITVRHRGGGSKRQLRMVDFLRDKSGIPAKVSAIEYDPNRTARLALLVYADGEKR
                     YILAPLGVKVGDVLVSGTQAEIKPGNNLPLANIPVGTTIHAIELQPGKGAQIGRSAGT
                     SAQLLAKEGKYAHVRMPSGEVRLVLQECSASIGQVGNVDHSNVKLGKAGRARHMGLRP
                     EVRGTAMSPRDHPHGGGEGRSSIGMPGPKTPWGKPTLGYKTRTNKRTDQMIIRHRSKA
                     KRR"
     gene            complement(14871..15176)
                     /gene="rplW"
                     /locus_tag="PKL60_02295"
     CDS             complement(14871..15176)
                     /gene="rplW"
                     /locus_tag="PKL60_02295"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNW94998.1"
                     /translation="MSNSVFDILRRPVVTEKSHYQAGKLHQYVFEVASDATREMVKTA
                     VETAFDVNVLRVNIINLPAKSHRNSRTRRLALRTGGYKKAIVTLAAEDSIPVFEGVE"
     gene            complement(15191..15820)
                     /gene="rplD"
                     /locus_tag="PKL60_02300"
     CDS             complement(15191..15820)
                     /gene="rplD"
                     /locus_tag="PKL60_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNW94999.1"
                     /translation="MKADVYNLQGEKSSTVELPDEIFAAKVNVDLMNQAYLRQLANAR
                     LGTHSTKRRGEVAGGGAKPWRQKGTGRARQGSTISAQWVGGGRIHTPKPRDYSQLMPK
                     KMRRAALRSALTVKASENGLVLVSDLALAEPKASVFSKAIKALAGDARTLVLVPDKSE
                     TYTNVLKSGRNLAESKLLLANYLNIRDLLQYEKVIIPLASLEVIKSFLG"
     gene            complement(15850..16482)
                     /gene="rplC"
                     /locus_tag="PKL60_02305"
     CDS             complement(15850..16482)
                     /gene="rplC"
                     /locus_tag="PKL60_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNW95000.1"
                     /translation="MFKGLIGRKIGMTQIFDADGAAVPVTLLEAGPCFVSQVKTEETD
                     GYNAVQLAFGEAKPKRLTKAHLGHLKTNNLPPVRVLREFRTKKLDGVNAGDKLDASVF
                     AEGEHVDVIGISKGKGFAGAVKRHGFAGGPRTHGQSDRLRAPGSSGSTTTPGRVFKGK
                     RGPGHMGDVQVTSSNVLVALVDPERNLIAVHGSVPGPKGGTVIIKEARKQ"
     gene            complement(16733..17041)
                     /gene="rpsJ"
                     /locus_tag="PKL60_02310"
     CDS             complement(16733..17041)
                     /gene="rpsJ"
                     /locus_tag="PKL60_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNW95001.1"
                     /translation="MAKQKIRIRLKAYDHRILDQSAKRIVETAERSGAKVLGPIPLPT
                     KLERYTVRRSTFIDKDSQEHFEIRTHNRLIDVLEPDSKTIDMLMRLNLPAGVDIEIKI
                     "
     gene            complement(17136..19208)
                     /gene="fusA"
                     /locus_tag="PKL60_02315"
     CDS             complement(17136..19208)
                     /gene="fusA"
                     /locus_tag="PKL60_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNW95002.1"
                     /translation="MEDFPLEKYRNIGIIAHIDAGKTTTTERILYYTGKTYRLGNVDD
                     GTTVTDWMAQERERGITIVSAAVTAEWKGYRINLIDTPGHIDFTAEVQRSLRVLDGGV
                     VVFDAVQGVEPQSETVWRQADRYQVPRICFANKMDRMGASYSRTIESIKRRLGATALA
                     MQIPIGEEAAFSGVINLLEEEAVYFDDNGGKDPRVEPIPAEYREKAARKRAQLVEKIA
                     ELDDALIEKFLEGESISIAELKAALRQGVLDARVTPVFCGTSLRNKGVQPLLDAVVDY
                     LPSPLDVPAVKATLLRDDSIVQRSPDPTEPLSALVFKIVTDPYMGRLAYIRVYSGKVR
                     QGEAVYNSSKGKKERVGRLLRMYADRREDIEELGAGDIGAILGLKFSFTGDTLSDSSA
                     PVVLESISFPDPVISVAIEPKTKADQEKMSDSLVKLAEEDPTFKIRQDENTGQTVISG
                     MGELHLDVLVDRLLREFKVQANVGKPKVAYREAITRPVSNVSYKYTKQTGGHGQYGHV
                     VINLEPMENGSGIVFENLIRGGAIPKEYIPAIEKGIYEAAESGPLAGFPMTDFKVSLI
                     DGSFHEVDSSELAFRMAAIFAFREATEKAAPVLLEPIMKVEITVPEEYTGDVIGQVNA
                     RVGNVLGMESQFGNAQMIHAEIPLAEMFGYATELRSATQGRGVFTMEFKHYAQVSESV
                     RKKILGLT"
     gene            complement(19241..19711)
                     /gene="rpsG"
                     /locus_tag="PKL60_02320"
     CDS             complement(19241..19711)
                     /gene="rpsG"
                     /locus_tag="PKL60_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNW95003.1"
                     /translation="MSRRIKPEKREILPDVRYNNTHIQMMINRIMLNGKKSTVTSLVY
                     DAIDMIAAKTGKDGVEVFEQALKNVSPVMEVRPRRVGGATYQVPMEVAPERRLTLAMR
                     WIISASRARSGKSYSEKLAGELMDAAENQGASIRRKEETHKMAEANRAFSHFRM"
     gene            complement(19791..20207)
                     /gene="rpsL"
                     /locus_tag="PKL60_02325"
     CDS             complement(19791..20207)
                     /gene="rpsL"
                     /locus_tag="PKL60_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNW95004.1"
                     /translation="MPTINQLVRNGRKTKHQKSKSPALQYTLNSEKQRRTRQPKGAPQ
                     KRGVCTIVRTMTPKKPNSALRKIARVRLSNGMEVTAYIPGEGHQLQEHSVVLVRGGRV
                     KDLPGVRYHIVRGTLDTTGVADRKRARSKYGAKRGK"
     gene            complement(20342..24253)
                     /locus_tag="PKL60_02330"
     CDS             complement(20342..24253)
                     /locus_tag="PKL60_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNW95005.1"
                     /translation="MTDLMPPKTYARIPIGLELPQLIQVQLDSFQRLKDEGLKSLFNE
                     VSPIVSYGNDLRLHFPSDTPISREFGLKFWFDAPKNSIEECLERDLTYACPLYVSVLL
                     EGNDISDKVKQDIFLGDFPEMTPKGTFIINGTERVVVSQLIRSPGVYFEAEVDRSTAR
                     ELATAKLIPDRGAWMEFETRKNDSIFLKFNRKRTVPITVFLRALAAVDDGMPDSLLKT
                     GSNEEIMELFKDVDNNPDHHFIETAFDEEPAYDLSSGLSISEAALIELFKKLRPGEPP
                     TLDNARNHLVEQLFDPRHYDLERVGRYKLNQKLDLVDDIPVSHRMITKYDVVRLVRHM
                     IKINNGTANADDIDHLGNRRVKTVGELIQNKLRVGLRRMERVIKERMSIRDQGGSLSP
                     VSLVNIRPLVASLREFFGSSQLSQFMEETNPLSELRHKRTVSALGPGGLRRERAGFDV
                     RDVHFSHYGRICPIETPEGPNIGLIGRLAAYATVNEFGFIETPYRKVRHYLEGSDPNL
                     VNHLLRKDIVDPATNEVLFKAETRVTPEMAAKISTLGLKEVWVRPFVTDEYVYLSADA
                     EDKYVIAQANAPLNEYHEFLHRNSCRYHSTFAIYDYEFIDYMDVAPNQVVGISAALIP
                     FLEHDDANRALMGSNMQTQAVPLLRPEVPIVSTGMEAAAVADSGQVVIAEEESEVIKV
                     TGDKLVLRQADGTIREHNLRKYQRSNQSTCIDQRPAVSKGQIVHKGDVIVDSSSTEDG
                     KLALGQNVLAAFVAWEGYNFEDAILISERLVEEDRYTSVHIEKYEVEARDTKLGPEEI
                     TREIPNVGDEAVRNLDESGIIRIGAEVGPESILVGKITPKGEKELTPEERLLRAIFGE
                     KSRDVKDTSLKMPHGERGIVVDVKVFTREENADLSAGVDKMVRVSVAQRRKVMAGDKM
                     AGRHGNKGVVSMVVPVENMPFLEDGRPVDIILNPLGIPGRMNIGQVLETHLGWAAESL
                     GFRAITPVFDGASEEEIEAELARAWMINEAWRVTGEKAWEWLDRVGAYSAEDLKDDEE
                     ARLIYLANALASTGIDVQELMSNRLLARRTALHEWLKAEAYDPASITAFPEDNIAVSE
                     RTAQDERAVRACLNLWAKSLGLELDPNLSMEDLRKAAYDLMLETGNPVPIIGKQVLRD
                     GKTGDRYDQPVTVGYMTFMKLHHLVEDKVHARATGPYSLVTQQPLGGKAQFGGQRFGE
                     MEVWALEAYGAAHTLQEMLTVKSDDVVGRENTYQAIVTNKPIEAPGTPAAFKVLVKEL
                     QSLGLAVESIMEDGEVVSYGKDNERRSGLLPTGLLDSHSGA"
     gene            24720..24911
                     /gene="rpsU"
                     /locus_tag="PKL60_02335"
     CDS             24720..24911
                     /gene="rpsU"
                     /locus_tag="PKL60_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNW95006.1"
                     /translation="MASVTLRANESQDSLLKRFRKKVVKSGVLSTVRRKRWFISKSEQ
                     RRMDKKKAIRRSYRKSYEA"
     gene            24933..25184
                     /gene="infA"
                     /locus_tag="PKL60_02340"
     CDS             24933..25184
                     /gene="infA"
                     /locus_tag="PKL60_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNW95007.1"
                     /translation="MAKEEEKITVDGTIIEALPGTQFRVRLENGHEVMAYLSGRMRRY
                     YIRILLGDSVRVEMSPYDLERGRIVYRQRRPGSSDLQEF"
     gene            25261..26235
                     /locus_tag="PKL60_02345"
     CDS             25261..26235
                     /locus_tag="PKL60_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW95008.1"
                     /translation="MPPPESQDSYLHIRRLNPNRDLNAVADLVEEAFELRDDPEGAWV
                     LRQMRAFARRAQSYPDVFSPPGAPEGFVWEEDGKIIGNISVVKYFQAEKRRVLIANVA
                     VARDSRRRGIASALTRHALRYIGQLPRSEVWLQVRSENLAAIQMYTQLGFEFIYPLSQ
                     WRSSPIRLFPVPARTSFPDSLRAAARKSADWKLQKQWLALNYPAQTRWYLNVNLDWFS
                     PWSFFVPASWQSLASLRHYSLRDGPRLRGVLTWQKTRLSSDQLWLALPEGPQEDLHAE
                     MLLDALQKIESPERSLLLEYPRGRAQMGIQSAGFTLARNLDWMKLKNS"
     gene            complement(26293..26484)
                     /locus_tag="PKL60_02350"
     CDS             complement(26293..26484)
                     /locus_tag="PKL60_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95009.1"
                     /translation="MDNEKEPVTTTIAETENYLAWRADEPDNESTYHLELNNVTLHFF
                     KEEWQEFLDLIETIVDEEL"
     gene            complement(26563..27888)
                     /locus_tag="PKL60_02355"
     CDS             complement(26563..27888)
                     /locus_tag="PKL60_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNW95010.1"
                     /translation="MAEPTDPEPLKEGAQTHTEKAVSPRWSKNTKLAISFAGILLVFG
                     LIIKFQNYLSMILTALLIVFLFTPMITFIQKHLKLSWRLATTLVYALMALILLGLLTW
                     GGITLFGQLQNLIDLLSSALSGLVKNLEQWSNQEVVWGPFSFFVPKLTATYLSDLLVG
                     QVQSILGEAGSLIGKVLSGGANLVFRLFMMYLISYFLTSETDGVKKQKISFGLPGYEK
                     DIQRMGKEISNIWNAFIRGEFLVVGSAIFIYSILLSGLQMPYALGLALIAGLGRFVPY
                     VGAWVSWLTFAIVALITRPTPFGLLPIVYMAVVVGVALVVDNILDNVMTPKVMGNALK
                     VHPAAVLITALIGSQLLGIIGVILAAPVFATVKLLTHYVFHKLNDQDPWEGISYSRWR
                     KELGLTRFFRYLKKKIPLWFKQARVSIAKGWHKVINRGTPPSKRPEKRI"
BASE COUNT         5612 a         8121 c         7560 g         6632 t
ORIGIN      
        1 tgtaggtcgg ggccttgcgg gcgcgcttga gacctggctt cttgcgttcc ttaacgcgcg
       61 cgtcgcgggt gagcagacct ttctcgcgca gaccaacgcg ggcgccttcg tccatgctga
      121 ccagggcgcg ggcaaggccg agcttgactg cgtcgcgctg accgctgacg ccgccgcctt
      181 ccaccttgac cgttacatcg gtcagggagg ggttttgccc ggctgcttcc agcgccttca
      241 gaatcatggg agcgtcgccg gtgcggggga agtacgcatc cagtggcttc tcgttgacgg
      301 tgaacacgcc gctgcctgcg gttatgcgga cgcgggcggt gctttctttt cgtcgtccaa
      361 cagcttcaaa atattgtgcc ataatacctc tttaattatt ccgaaatcgg tttggggtcc
      421 tgcgcctcgt gcgggtgctc gggaccggca tagattttca ggttggccag atagcgcacc
      481 gtcaggcggt tcttgggcag cataccgcgc actgccgcgc gaatgaccgc ctccggcttt
      541 acttccatct gacgcttgat cgtgacagac ttcagaccgc ccgggtagag cgaatgacga
      601 tagtacttct tttcttcagc acgggtctga ttaaaagtaa tctgcgcggc attgattacc
      661 accacatggt cattcagaac cacaccgggg gtatagtccg cgcggtgctt gcccgtcaac
      721 aacacggcga tgcgggtcgc gagtctgccc aacgtttcgc cggcagcatc cacgagatac
      781 cactcaccga cagtttgtcc tttaggaacg tatgtttttt ccactgttac tttctccgtt
      841 ttatcaaatc cacactctgg cgtttcacgc cggagcttat ttcctaccat cctccggata
      901 agcgacttcc cacagggtca atccccgggc aggcgccagg ctgacaatcc ctgtttcgcc
      961 gctttccaga cctcggctta cgatttcctc gggcaggttc cccagcccga tttgcacgac
     1021 gatgaacaca attcggcgca ccatatggta caaaaaagcg ttaccgcaga tgtcaaaggc
     1081 tgcctcctgg ctgccgccct gccactccgc gctgaaaacc ttacgcatgg tggtggcatc
     1141 ctccttcagc gccttaccaa atgccctgaa gtcgtgttca ccgaccaaaa gcccggctgc
     1201 gcggttcatc ttctccaggt ccggcatgca attcaagcgc cagctaaagc gatccaccag
     1261 cgggttacgc gcgcgctgcg cgtaaacctg gtagcggtac gtgcggctca gcgcgtcgta
     1321 ccgcggatgg aaagccagcg gcacttcttc ggcggcctgc acagcgatat ccgcgggcag
     1381 gtgagcgttc agcgcggtaa ccagcgtttc cgtgccgtgc ttccagtcca gggaaaagct
     1441 gataacctgc ccccgggcgt ggacgccgct gtcggttcgg ccggcgggca tgactgcccg
     1501 gccctgccat cccaacttgc gcagggcgct ttcaaactcg gcctgcacgc tgtgcctgtt
     1561 ctgctgccgc tgaaagccgt ggtatgcgct gccttcgtaa gcgagaataa gtttgtaatg
     1621 tgccatgcct ttcaacttcc agggcctgaa aggcgaaaac ccttcatgcc gcgttactcg
     1681 gaaacgagtt caagcaggac catttccgca ttgtcgccga ggcggggccc aagtttgagc
     1741 aggcgggtat agccgcccgg gcgggtcttg aagcgcggag cgatgtcgtc aaacagcttc
     1801 ttcacggtgt ctgcgctggc atttccaagt tcagccgaaa tctggcggcg ggcgttcagc
     1861 ttttcaactt cgctcgcgct caggctgttg cgggcaaggg taatcatctt ttcagcgtcg
     1921 ggctgaatgg actttgcttt tgccagcgtg gtctgaatct tttcgtgttc aaataattgc
     1981 ataatcatcg tcttgcgcat ggctgtgcgg tgcccgccgc ttctgccaag gcgatatcct
     2041 tttacctgat gtcgcataac taactactcc gctttttctt cttttaggta gcctttttcg
     2101 atcattttgg ctcttaattc gaccaggctc ttctcgccaa agtttcggat ggagagcatg
     2161 tggttttcgc ctttggcaag catctcacgc acatcgcgta cggaggagac gccggtgcgc
     2221 ttcagggcgt tgtacacgcg cacggtcaaa tcaagtgcct cgatgggggt ctcggctgct
     2281 tcaatgccgc ccatatggtc ggcttcttca acttcgactt cgctttccag catatccccg
     2341 ctggtgccgc taatgaagct gaactgacgg atcagcaggt ccaccgcgct ctgcagagcc
     2401 acttccggag gaactgtgcc gtcggtatgg atttccaggt tcagcttgtc gtaattggtg
     2461 ttgtgaccaa cgcgcgcgtt ggtgacggac caattgacgc gccgcaccgg ggagaaaatc
     2521 gcgtctaccg gcaaaacgcc gataggcagg cgctccgcgc gttcgccagc gggcatgtac
     2581 ccgcgtccgc gttcgaccgt gaattccatt tccacatgcg ccgcgttgtc ggtgatgctg
     2641 aaaaggtaca ggtccgggtt gataatttca acttccggcg ggcagataat atctgcggcg
     2701 gtgtaggtgc cttctccgcg cacgtccaac tggaggtgcg ccgtatccac atcataaagc
     2761 ttcatgcgga tctgcttcag gttcagcatc acctggataa cgtcttcgcg gacgccggga
     2821 atcgtgctga actcatgcat cacatccgta atgcgcacag cggtgattgc cgcgccttcc
     2881 aaggaggaaa gcaaaacgcg gcgaattgcg tttccaaggg taattccgaa accgcgttcc
     2941 agcgggctga tcgtgaactg cccggtctca cggctctgtt ttttaccttc tacttttggt
     3001 gttaccatat catttcttcc actcacggtt agcccctccg ccagtctctt gattcgagag
     3061 actgaataaa tcggtgatta gcgcgaataa tattcaacga tcagctgttc gttgaggtta
     3121 ccgtcaattt caacacgctc aggcaagcgc agcattctgc cgctcaggtt ggtcatatca
     3181 cgcgccagcc aacccggtac agttctgcct tccgcttctt cccgcaggtt tttgaaatag
     3241 atggtatcac gggacttttc tcgtacagag atgacatcat caacggaaac tccataggac
     3301 ggaatgtccg cgcgttcgcc gttcaccaaa aagtgaccgt gggacaccag aatacgggcc
     3361 tgcggacggc tggcggcaaa accaagacgg tagactacgt tggagagacg tgtttccaga
     3421 atctgcagca ggttgttccc ggtcagcccg cgcattttca gggcctggcc gtagaagcgg
     3481 cggaactggc gttcatagat gccgtacacc cggcgtgcct tctgtttggc acgcagctgc
     3541 ttggcatagt cggattcgcg gccgggccgc gcggagctca tttttccatg cattccaggc
     3601 ggataaccgc gtttttcaaa agcgcatttc ggtgagaagc agcgttcccc tttcaggaac
     3661 aacttctcgc cctcgcgccg acacaaacga catactggat cagtgtatct tgccataatc
     3721 aattttcctt tttcaaatac tacacgcggc ggcgttttgg cggccggcag ccgttatgcg
     3781 gaaccggcgt cagatcggat atcaggcgaa ccttgatacc cagggattgg acagcacgaa
     3841 tggcggcttc gcggccggga ccaggacctt tcacaaacag ctccacctcc tgcacgccca
     3901 tggtttgagc ggtcttgatg gtctgttccg tagccattct ggcagcgaaa ggcgtgctct
     3961 tgcgcgaacc tttaaaccca gcgcttccgg agctccccca gcacagggtg ttgccctgca
     4021 ggtcggtcac cgttacgatg gtgttattaa aagacgcgta gacatgcacc tgcgccgtcg
     4081 atacctggcg ttttactttg cgggcactgg tcgttcgtcg tgctgtgcgt gcttgtttag
     4141 ccataattct tcctcactca atctcttctt gtccggcaag ccaaaagcct gaagtagccg
     4201 caggggagaa aggaaaccct tctccaggct caggctgcca gttcaatgct acttcttggt
     4261 cgctccacgg cggcggccgc ggccggcaac ggtcttcttg ggacccttac gggtgcgcgc
     4321 gttggtacgc gtgcgctgac cgcgagtcgg cagatttcgg cggtggcgca gaccgcggta
     4381 acaaccgatt tccaccagac gtttaatatt catctgaacc tcgcggcgca gatcgccttc
     4441 aaccttcagg ttctgggaaa tataatcacg cagcttggac acttcgttct cgggcaggtc
     4501 cttgacacgg atgtcaggat ccaccccggt cgctgccagg atttcatcgg cggtttttgg
     4561 gccgatacca taaatgtagc gcaaaccaat ctgaatgcgt ttattacgcg ggaggtcaac
     4621 accttctata cgagccattg tatgattcct tatccttgtc gctgtttgtg tcttgggttg
     4681 gtacaaataa catagagccg accgtcgcgt ttgacgatct tgcactttcc acagcgcttt
     4741 ttaattgaag ctgacacttt catcagtaac tccttatgct tatccgcaaa gattctatta
     4801 tatcttctct tcagtctctt ggcaagacct gttttttcat aaacgcgtca aaatttccgg
     4861 cccgttcgcc gtaattgcca ccgtgtgttc ctggtgcgca gtcagcgagc ggtcccgcga
     4921 aatcactgtc cactgattct tcagttcgcg cgtataacgc gtcccgacca gaaccatcgg
     4981 ctcaatcgcc agcgtcatcc cttcccgcag cagcatgccc tgcccgggtt ggccgtaatt
     5041 cggcaccagc ggactctcgt gcatctcccg acccactccg tgccccgtgt acaaatgggt
     5101 cacgtggaag ccgtgcttca gcaccaggct ttccacagcc gcgccgatat ccccgagatg
     5161 attgccgggc accatctggg caatccccgc gtccagggct tgttttgtga cctctatcag
     5221 ccgctgggct tgcgaggaaa tttctcctac gccgaccgta aaagctgaat cgcccacaaa
     5281 cccctgataa accgtgccgc agtcaatcga gatgatatcg ccctctttca acttgcgttt
     5341 gtccggaatg ccgtgcacca tctcttcgtt cacgctggcg cagatgctgg ccggaaacgg
     5401 gtagggaccc gggtaatttt taaagggcga atacactcca tatctgcgct ggacagcttc
     5461 gacgacttcg ttcagttccg ccgttgtcac tcccggcgcg atggctgctt tcgcggcttc
     5521 gagcgcctct gcgttaatcc ggccggcttc gcgcataatc gcgatttccg ccgcagattt
     5581 gatgttaaga cttccttcca tcagcagccc gaaccaatcg cgttcaggat atcttcggta
     5641 accctgtcaa tatcctgtgt cccatccact tcaaccagaa cgccggccgc ctggaagaaa
     5701 tcaatcagcg gggcggtttg cgctttgtac acgtcaatgc ggtgctgcac cgtttccggt
     5761 ttgtcgtcat cgcgctgata aagcgggctg ccgtccacat catcaagcca ttgaaccttg
     5821 ggcgggttgt aaagcgcgtg gtaaacgcgc ccgctggggc tcatccatct gccgctcagg
     5881 cgctcaacca ggacttggtc gggcacatta atcaagggca caaggtcaat ttttccgttc
     5941 atttccgcca gaatgccgtt caaggcttcg gcttgcgcgg gcgtacgggg gaaaccgtcc
     6001 agcaccgcgc ctgaagcaca gtccggtcgg gagaggcgtt ctttgaccat cgcgatggta
     6061 acgtcatccg gcaccaattc gcccttggtc atgtaggtct gcgccagttt gcccagatcg
     6121 gtttgccggc tcaaattctc gcggaacaaa tccccgctgg agacgtgcac caggccgagc
     6181 ttttcagcaa tcaccttagc ctgggtgcct ttgccggccc cgggaggacc taacatgacg
     6241 atgtaagtcg ccttctcatt catctcaacc tcagctatta atcagcctgt cgtcataacc
     6301 gtgcatgcgc agttcagttt caatcagttg gacggtttca cgtacggtac caaccacaat
     6361 cagcagaccg gaggaggaaa gcagcagcgt cgctgagctt cctgccggaa cgacggcgct
     6421 gaacaaataa ggcatcaccg cgacgaaacc aaggaagaag gcaccgggca gcgtaattct
     6481 gcgctgcacg cgggtcaggt agtcctgcgt gggttttccg cgcacgacgc ccggaatttg
     6541 cgcgccttgt ttcttcaggt tatcgccgta gttttgctgt gtgaacatca cgtccgtata
     6601 gaaataggta aacaacacca ccagcacaaa gaacattacc ggataccagg cggcttccgc
     6661 gccaaaagca cccgtcaccc aattcgccga cttggccagc caggctgttt ttgagttcaa
     6721 aaagaacgaa gccagaatcg ccggaaacgt caggaaggac tgggcaaaga taatcgggat
     6781 catgccggcc atgttcacca tcagcggcag gttgctgcgc accggcatag acatgcggtt
     6841 tccaattctg cgtccgggga acatcaccgg aacgttgcgc cgcccctgct ggacgtacac
     6901 aatcgcgaag atggtgatga tcaggataat gaccacgatg aagatcatcc accaggattc
     6961 cttgctggaa atcatcttaa gtaaagactg cggcaatcgg gaaacgatac cgctgaaaat
     7021 aatcagcgat aagccttggt tgcgcagacc aaactcggag ataagctggc caatccacac
     7081 gccaagcatg gtgccagcca ccatcgcgaa aatcgcggta agcgtgggca tgagctttcc
     7141 ggcggtgaaa ccccagttgg taataatgga gctgccgagc atctgattga agatgttaat
     7201 ctggccaaag gcggagagca gcgccatagg gatagtcaga atgaccgtcc atttttccat
     7261 ccagatacgg ccttcgcggg gatcatcctg cattttcttt tccagcgcag ggataaccgg
     7321 ctggagaagc tgcaaaataa tctgagcggt aatataagga tagacgccca tcgccaggat
     7381 cgagaaattg gagaccgcgc cgccgctcaa catatccagc agattgatca ggttgccttg
     7441 cgtgcctttc agagtggtta acgccgctac cgtagccctg tcgataccgg gcacggggat
     7501 atttgccgcc aggcggtaaa tcgccagcag cagcagcgta atcaacagct tattgcgaat
     7561 atcttcagat ttccacagaa agcgccatga cgaccgtttc atgaggtttt gctccttttg
     7621 gcctatgcca acgcgatcgt ttccacgctg ccgcctgctt tttcaatctt ctggcgggcg
     7681 ccatcggtca ctttgtgcac tttgattctc agggcaacat tcacatcgcc ccgtcccatc
     7741 agcttgatag gtttgttctt atccttaagc aggccggctt ctgccagcgc ttcgggggtc
     7801 acttcagcgc ccgcggcaaa gccgtgcagg gcgtccaggt tcacctcggc atagcgcacg
     7861 cggttcagcg ggctgaaacc ctcaccgcgc aggaaaggca gtttgcggaa cagaggcagg
     7921 ttgccgcctt ggtgatacag acggacaccg ccgccgttgc gggagttttg gcctttgatg
     7981 ccgcgcccgg aagtttttcc ggagccgctg ccgtggccgc gcccaacgcg tttgcgcagt
     8041 ttcttggaac cctcattggg ttttaagtca tgtagttgca ttacttctcc tcattcactg
     8101 tttcaatctc aaccagatgg gagaccttcg tgagcatccc acgcagcgcg ggagaatcct
     8161 catgttccac aacctggttc acatgatgga aacccagcgc cttgagggtc gctttctggc
     8221 ggacaggata gcccgtgccg ctcttcttca gagtgacgcg cactctgctg ctttttttgg
     8281 tttgtttagc catgcttcct ccgctcccag aaaggtgtca cgtccgcgac atccttgccg
     8341 cgttcagcgg ccacagtctg gggggacttt agttgaccga gcgcatcgaa ggtagcctga
     8401 acgatattga gcatattgtt gctgcccatg gatttggtca gaatgtcctt aatgcctgcg
     8461 gcttcgcaaa cagcgcgcac gccgccagcc gcaatcacgc cggtcccggg ggaagcgggc
     8521 ttgagcaaaa ctttggcgcc gctttgggtg ccggtaactt cgtgggcaat ggtcgtaccc
     8581 agcaagctga cctcatgcat attcttgcgg gcatgctccg tcgctttgcg catcgcgtcc
     8641 gagactgcgc cagctttgcc gatgcccagg ccaacttttc cgttgttatc gcccaccacg
     8701 agcgtaacgc ggaaggagaa cctgcgaccg cctttaacaa ccttggcaac ccgggcgatg
     8761 tcgattacgc gttcatcgag agtagtttct tcgttttgat aatcttccat catttcctca
     8821 gcttaaaatt ccagaccgcc cttgcgcgcg ccgtcagcca gagcctttac gcggccggta
     8881 tagtaataac caccgcgatc catcacgacg gacttaatgc ctttggcaag cgcccgcctg
     8941 gcaatttcct cgcctaccag tacagcctgt tcggccttgg gcttgccttt gaccgcgtcc
     9001 cgcagaaggg tgtctacagt tgaggcggag accagggtcg tcccgctgtg atcatcaatc
     9061 acctgcgcgt aaatctcagt aatgctgcgg tacacattca agcgtgggcg ttccggagtt
     9121 ccgaaaacct tcttgcggac ccgcgtgtga cggcgtaatc gagctgcgtt tcttgattta
     9181 tttgccatag tctttacctc tacttaccgg tctttccagc cttgccagcc ttcagtttca
     9241 caacctcacc ctggtagcgg atccctgtac ctttgtaatg ctcaggcgga cgaatctcgc
     9301 gcaggttcga tgcaacttgc cccacctgct gtcggtcccg acctttcacc ttcaccaggc
     9361 gggttttggt ctccacctca aaggtaatgc cttccggcgc gggatacgtg acgggatggg
     9421 aataacccac gttgaggacc aggtccttcc cgttcagttc cgcgcggtag ccaacaccgt
     9481 tgacttccag gacgcgttcg aaacctttgg aaacgccgtt gaccatattg ttcagcagcg
     9541 cgcgcgtggt gccgtgcaaa gcgcgcacgc gcggttcatc attaggtcgt tcaataatca
     9601 aatggccatc ttccagcttg atgccaattt ctgaagaaaa ttcctgctcc aattgtccga
     9661 gcggaccctt aacagaaact ttatttcctt caattttgac ctcaacgccg gcaggaatgg
     9721 cgatgggtaa tctaccgatt cgcgacattt tatgctccta ccaaatcttg cagaggatct
     9781 cgccgccgac cccaaccttg cgggcgcgct gaccggtcat gatccccttg ggggttgaca
     9841 aaatcgcgat accgatgccg gaaagtaccc acgggatttc gtcccgggag gtgtacacgc
     9901 ggcagccggg agtgcttacc cgctgcaggc cggtaataat cggacggcgg tggcggcgtt
     9961 cgccgatgta cttcagcttc aggcgcaaaa ccttgctgcc gttggctttt ccatcgacaa
    10021 cttcatagct ctcaaggtag ccttcttcct gcaaaatgcg ggcgacttct tccttgattt
    10081 tggaattcgg catcaccacc aggctttgtc cggccatggc gatattgcgg attctggtta
    10141 acatatcggc aattggatct gaaatagtca tgttactccc tccaggccaa attaccagga
    10201 tgatttctta acaccaggga tttttccctg aagtgccagt tctctgaaac aaattcggca
    10261 aagaccaaac ttgcgcatat atccgcgcgg tctcccacaa atccggcagc ggttgaccac
    10321 ctgattgggg tattcgcgtc gcatttcgcg gtacttcata cactttttag ccattagttt
    10381 cctttcctga agggcatgcc gaacttgctg agcagctgcc gcgcgtgatc gtcattctca
    10441 gccgttgtca cgatgcttac ttccatccca cggactttgt caatcttgtc atattgaatc
    10501 tcagggaaaa tcaactgttc ctgcagaccc aaggtgtagt tgccgcgtcc atcaaacgca
    10561 tccgcggaaa ttccgcggaa gtcgcggacg cgcggcagcg cgatgttgat caaacgatcc
    10621 agaaaagccc acatcttctc accgcgcagg gtaactttca cgccaatctg gcgcccttcg
    10681 cgcagcttga agttggaaat cgctttcttg gctgcgatta caaccggctt ctgaccggtg
    10741 atggccgtca tgtcgctcac ggcggcttcc agcgctttcg gattgtccag cgcttccccg
    10801 acgccgatgt tgaccaccac tttaacgatc ttgggtgtct gcatcacgtt cttcaacccc
    10861 agttccttga acagagccgg cgcgatttcg ttcttgtatt gttcctgtag tttattcatt
    10921 gcttctcacc tgcgccttat ttgcctgagc cgctgttatc gtcggcgggc ttgtcggctg
    10981 cgcgcttcct ggcggcgggt tttgcttctt tcctgacagc cttggcgggt gccttcctgg
    11041 ccccagtttt ggtaggcctg ccgggctttt ccgctttgcc atcggctttt tccgcgcctt
    11101 gcttcacaag ttccacgttg gaaatgtgaa tcggagcttc aaattcgcga atccctgccg
    11161 ggatggtttt gctgccggac tgcgtctgct tctgatgctt cttcaccagg tttacgccct
    11221 gcacgaccac gcgctcttct ttgggcaaaa cacgaatgac ctcgccggtc tttcctttgt
    11281 cactttcatc accgcgggtg accttgacct gatcaccttt tcgtattttc attttcatct
    11341 gaacactcct actcaatggc tacagcactt ccggagccaa tgaaacaatt ttcataaagc
    11401 ctttttcacg cagttcgcgt gcgaccgggc cgaaaatacg ggttccgcgc ggattcactc
    11461 cgtcgttatc caaaatcacg gcggcgttgt catcaaaaca gatgctcgaa ccgtccggac
    11521 ggcggtattc tttgctgacg cgcacaatca cggctttgac aatttcgctt ttcttgacag
    11581 acgcctgcgg agaagcgctc ttgaccgtgc caatgacgat atcgcccaca taggcatagt
    11641 gccggcgggt tccgccaacc acatggaaaa ccagaagttc tttggcgccg gtgttatctg
    11701 caattgttaa ccgagcctct tgctgaatca tgccgcctcc ccttcggaaa tatcatcaag
    11761 ctcgcccgct tcgcggactt cgactctgat gatcttctca acaatccaat gtttggtcgc
    11821 agaaagcgga gcgcattcca caatgctgac cttatcgcca acgctgcagt tcaactcatc
    11881 atgcgctttg taaactttgc tgccgcgcac cactttgtgg tagagcggat gctgatacgt
    11941 gcgggaaacc tccacgctga cggtcttggc cgttttggcg ctggtaaccg taccgattag
    12001 acggcgacgt ttgttcatct taaccttcct tccctttacg ttctcgctgc gcaaggatag
    12061 tttcaaaacg cgcgatctga cggcgggtaa ccttaaaacg gctggtgtcg gtcagctgac
    12121 cggtcaccat ctggaaccgc atattcatca gctcctgccg ggaatccatc aacttattct
    12181 taatttcttc cgtagaaagt tctcttattt ccttggtgtt catgaacctt ctcctaccaa
    12241 ttctttcgta acaatcttgg tgcggaaccc aagcttatag cgcgcgcgct gcagggcttt
    12301 taacgcggat tcctcgtccg tgccgcctac ctcgaacatc actctgcccg ggcggattac
    12361 ggctacccaa tggtccacgg aacctttgcc tttgcccatg cgggtttcgg cggcgcgctt
    12421 ggtgtaaggt ttatccggga aaacacggat ccacaccttg ccgcggcgct taagttcgtg
    12481 cacgatggcc cggcgggcgg cttcaatctg gcgcgcggtc aggtatccgc attccagcga
    12541 cttcaaacca taatcaccga agctgacttc cacgccgcgc tgggctttgc ccttcatgcg
    12601 gccgcgcatc tgcttgcggt actttactct ctttggcatc aacatagtcc actacctcac
    12661 aattacctaa taattactcg ctgatataca cgccttcggt ggcttcgatt ttctcctcag
    12721 ccaggcccat gacgatgccg cgatagatcc agaccttgat accaatctga ccgtaagtgg
    12781 tggaggcttc cgctcgggcg aaatcgatgt ccgcgcgcag agtctgcagc ggaacacggc
    12841 cttcgcgcag ggtaacccgc cgcgccattt cagcgccgga aagacgcccg gaaacggaga
    12901 ccttaatccc gccggcgccc tggcgcagcg cctgttgaat ggcgcgtttc atggccctgc
    12961 ggtagctgat gcggcgttcc aactgtccgg caatattctg ggcgaccaga tacgcgtcgg
    13021 tgtcggggtt cttgatttcc tttacgtcca ggtctacctt cttgccggtc agagcttcca
    13081 gctcctgacg cagaattttg acgctttcgc ccttcttacc gatgagcacg ccgggtttag
    13141 cggtgtgaag cgataccttc accttgccgg gatagcgctc aatttccacg ccggaaacgc
    13201 ccgcacgttc ggcgcctttc agcaccagct cgcggatgcg caggtcctga tgcagctgtt
    13261 cagtataatc gctgccttca gcataccagc ggcctaacca gggtttgttg atacccaggc
    13321 gaaaaccaat tggatgtact ttacgaccca taatttctcc ttaactctct ctccggtcct
    13381 actcgatctc acgcagaacc acggtcacgt gggaactgcg gcgcagctgg ggcttgaaac
    13441 ggccgcgggc gccaaaggat ctccatttac gggtaggagc ttcatctgcg aaaatcttgt
    13501 agacgaccag gtcttcccgg ctgacgccaa aattctcttc ggcgttagac atcgcggagt
    13561 aaagcagttt gcgaacaggt tcggctgctt tattcggcgt caggctcaga atgcccaggg
    13621 catcgacgac ctttttaccg cggaccagat ccacaaccag acgggctttc tgggcggaag
    13681 ttgagaggtt ggataagctt gcttttattt cctgtgccat ccctgcctca cttggtagac
    13741 ttttcctgaa catgcccgcg gaaccatcgg gtcggggcaa attcgccaag gcggtggccc
    13801 accatgttgt ccgtcacata gatcggaaca tgacggtgcc cgtcgtgcac agcgatggtg
    13861 tgaccgacca tctggggaaa gatggtgctg gcgcggctcc aggtgcggat gacctttttg
    13921 tcgcctttgg cgttcatctc ttcaatacgt ttcaaaagtt tcgggtctac gaaaggccct
    13981 tttttcaatg atcgagacat cttatcgtcc ttttctgctt ctcatccact actaacggcg
    14041 cttggccttg gagcggtggc gaatgatcat ttggtctgtc cgcttgttcg tgcgggtctt
    14101 gtatcccagg gtcggtttgc cccacggcgt ctttggaccg ggcatgccaa tcgaggaacg
    14161 tccttcaccg ccgccgtgcg gatggtcccg gggggacatc gcagtaccgc ggacttccgg
    14221 acgcaggccc atatggcggg cgcggccggc cttgccaagc ttcacgttgc tgtggtccac
    14281 attgccaacc tgaccgatag acgcgctgca ttcctgcaga accagacgca cctccccgga
    14341 gggcatgcgc acatgcgcgt acttgccttc cttggccagc agctgggcgg aagtgcccgc
    14401 agaacggcca atctgggcgc ccttcccggg ctgcagttca atcgcgtgga tggtcgtgcc
    14461 aaccgggatg ttcgccaggg gcaggttatt gccgggctta atttcggcct gcgtgcccga
    14521 gacgagcaca tcgccaacct tcacgcccaa aggagccagg atgtagcgtt tctcgccgtc
    14581 agcgtagacc agcagcgcca ggcgggcggt gcggttggga tcatactcaa tcgcgctcac
    14641 cttggcgggg atgccggatt tatcgcgcaa aaagtcgacc atgcgcagtt ggcgcttgga
    14701 gccgccgccg cgatggcgga cggtaatccg accaagggag ttgcggccgg cggtcttctt
    14761 cccggaaaca atcaggctgc gctcgggacg gtccttcgta atctcttcga aagtgtagct
    14821 ggtgcggtgg cgctgaccag gagtcttggg tttgtaaatt tttacggcca ttattccacc
    14881 ccttcaaaca caggaatgct gtcttccgcg gccaaagtca caatggcttt cttgtaccca
    14941 ccggtgcgca gcgccaggcg gcgggtgcgc gagttgcggt gagacttggc ggggagattg
    15001 atgatattga cgcgcaggac gttcacatca aacgcggttt ccacggcggt cttcaccatc
    15061 tcgcgggtcg cgtcgctggc gacttcaaac acatattggt gcagcttgcc agcctggtaa
    15121 tgcgattttt ctgtcaccac agggcgccgc aggatatcaa atacggagtt gctcattctg
    15181 cttcaccttc ctatcccaaa aagctcttaa tcacttcaag tgaagcgagc gggataataa
    15241 ctttttcata ctgcagcaaa tcgcggatat tcaggtaatt cgcaagcagc agtttggatt
    15301 ccgccaggtt gcgtccggat ttgaggacgt ttgtgtaggt ttcactttta tcgggcacca
    15361 aaacgagcgt gcgcgcgtcg ccagccaggg ctttgatggc cttggagaac acgctggctt
    15421 tcggctcggc cagcgccagg tcgctcacca gcaccaaacc gttttcgctc gcttttacgg
    15481 tcagagcgga gcgcagcgcg gcacggcgca ttttcttggg catcagctgg ctgtaatcac
    15541 gcggcttcgg ggtgtgaatg cggccgcctc caacccactg ggcggaaatc gtgctgccct
    15601 gacgggcacg gccggtacct ttttgacgcc agggcttggc gccgccgccg gcgacttcac
    15661 cgcggcgttt ggtgctgtgc gtgccaagac gggcattggc cagttggcgc aggtatgcct
    15721 ggttcatcaa atccacgtta acttttgccg cgaagatttc gtcgggcagt tcaaccgtgc
    15781 tggatttttc tccttgtaag ttataaacat ctgctttcat gataactttc tccattcatc
    15841 acaagccggc tactgcttgc gagcctcttt gatgattacg gttccacctt tgggacccgg
    15901 aacagagccg tgcactgcga tcaaattgcg ttcggggtca accagcgcca cgagcacatt
    15961 tgaggaagtg acctgaacat cacccatgtg accaggacca cgttttcctt tgaagacacg
    16021 tccgggggtt gtcgtcgagc ctgaggagcc aggcgcgcgc aggcggtcgg attgaccgtg
    16081 cgtgcgcgga ccgccggcaa agccgtgccg tttcaccgcg cctgcaaaac cttttccctt
    16141 gctgataccg atgacatcca catgttcgcc ttcagcaaac accgaagcgt ccagcttgtc
    16201 gccggcgttg acgccgtcca acttcttggt gcgaaactca cgcagaacgc ggaccggagg
    16261 caggttattg gttttgagat gcccgaggtg cgccttggtc agacgcttgg gtttggcctc
    16321 gccaaaagcc agctgtacag cattgtaacc gtccgtttcc tcggtcttca cctgggaaac
    16381 aaaacaaggc ccagcttcga gcagcgttac cggcaccgca gcgccgtccg catcgaagat
    16441 ctgggtcata ccgatctttc ttccaataag ccctttgaac atactattct cctgttaggt
    16501 ttcattcttt gcgagactgt cagcatgggc aagctgcatc atctccgcgc aattcagtca
    16561 atggaccctg tgtagcggtt ctgcccacgc gggtgcgcat tcttaaattg cctgttaagg
    16621 ttgccgtaag ggcaacctta tatttttacc acaaccctgc attctctgca agggctgaag
    16681 tggtcttctt ttgtaacgat tcccgcggcg cgcttgcgcg cccttggcaa ccctaaatct
    16741 taatctcgat atccacaccc gcgggcaggt tcaggcgcat cagcatgtca atcgtcttgg
    16801 aatcgggttc cagcacgtca atcaggcggt tgtgcgtccg aatttcaaaa tgctcctgtg
    16861 agtctttgtc aatgaaggtg gaacggcgca cggtgtagcg ttcgagcttg gtaggcagcg
    16921 ggatggggcc gagaactttc gcgccgctgc gttccgccgt ctccacaatc cttttggcag
    16981 actggtcgag gatgcggtgg tcgtatgctt tcaaacgaat tcgtattttt tgctttgcca
    17041 taatcttcct aatcaattct tagtgtgttg cttcgattta gaacctgcgc cggggcgccg
    17101 gttttgatct tctggttgga ttcgcgcttc cggggctacg tcagtccgag gatcttcttg
    17161 cgcacgcttt cggacacctg ggcatagtgc ttgaactcca tggtaaagac cccgcgtcct
    17221 tgcgtggcgg agcgcagttc cgtggcgtac ccgaacatct ccgcaagcgg aatttccgcg
    17281 tgaatcatct gggcgttgcc aaactgggat tccatcccaa gcacattgcc aacccgcgcg
    17341 ttcacctgac cgataacatc gccagtgtac tcttcgggaa ccgtgatttc cactttcatg
    17401 atgggttcca gcagcacggg agctgccttt tcggtagcct cgcggaaggc gaaaatggcc
    17461 gccatgcgaa aggccaattc gctcgagtca acctcatgga aggaaccatc gatcagggag
    17521 accttgaaat cggtcatcgg gaaacccgcc aaaggtccgc tttccgcggc ttcgtagata
    17581 cccttttcaa tcgcggggat atattccttg ggaatagcgc cgccgcggat caggttctcg
    17641 aagacgattc cgctgccgtt ttccatcggc tcgaggttga tgacgacatg accgtattgc
    17701 ccgtggccgc cggtctgttt ggtatacttg taactcacgt tggacaccgg gcgggtgatc
    17761 gcttcccgat aagccacttt gggcttgcca acgtttgcct gcaccttgaa ctcgcgcagc
    17821 aaacggtcca ccagcacgtc gagatgaagc tctcccatgc cggagatgac agtttgtccg
    17881 gtgttttcgt cctggcgaat cttgaaagtc gggtcttctt ccgccagttt caccagcgaa
    17941 tcggacattt tttcctggtc ggctttggtc ttgggttcaa ttgccacgga aatcaccggg
    18001 tccgggaagc tgatgctctc cagcactacc ggcgcggagg agtcgctgag ggtatctcca
    18061 gtgaaactga atttgaggcc caggatggcg ccaatgtcgc cggctcccag ctcttcaatg
    18121 tcctcacgcc ggtccgcgta catgcgcagc aggcgtccga cgcgttcttt tttgcctttg
    18181 gaggagttat agactgcctc gccctgcctc accttgccgg aatacacgcg gatatatgcc
    18241 aggcgcccca tataggggtc ggtcacaatt ttgaagacca gcgcggagag cggttccgtc
    18301 gggtccgggc tgcgctggac gatgctgtca tcgcgcagga gcgtggcttt cacagccggc
    18361 acatccagcg gcgagggcag gtaatccacc acggcatcca gcagtggctg cacgcctttg
    18421 ttgcgcaggc tcgtcccgca aaaaacaggg gtcacgcgcg cgtcgagcac gccctggcgc
    18481 agcgcggctt taagctctgc gatgctgatg ctttcccctt ccaggaactt ttcaatcagc
    18541 gcgtcgtcca gttcggcgat tttttccacc aattgggcgc gcttccgcgc ggctttttcg
    18601 cggtattccg cagggatagg ttccacgcgc ggatctttcc cgccgttgtc gtcaaagtag
    18661 acggcttctt cttccagcag gttgatgaca ccggaaaaag cggcttcttc gccaatcggg
    18721 atttgcatcg cgagcgcggt cgcgccgagc cggcgcttga tactctcgat ggtgcgcgaa
    18781 tacgaagcgc ccatgcggtc cattttgttg gcgaaacaaa tccgggggac ctggtagcgg
    18841 tctgcctgac gccagacagt ttcgctctgg ggttccacgc cctggacggc atcgaagacg
    18901 accacgccgc cgtccagcac gcgcaaagag cgttgaactt cggctgtgaa atcaatatgc
    18961 ccgggggtat caatcagatt gatgcggtag cccttccatt ccgcggtgac cgcggcggac
    19021 acgatggtga tgccgcgttc gcgttcctga gccatccaat ccgtgaccgt ggtaccgtca
    19081 tccacgttgc ccaggcggta ggttttgccg gtatagtaaa gaatccgctc ggtagtcgtg
    19141 gttttaccag cgtcaatatg ggcaataatg ccaatatttc gatacttctc gagcggaaaa
    19201 tcttccatga agaaacctta atagtcgcta tccgccggga ttacatgcgg aagtgagaaa
    19261 aagcgcggtt ggcttccgcc atcttgtgcg tttcttcttt gcggcggatt gaggcgccct
    19321 gattttcagc ggcatccatc agttcgccgg caagcttctc agagtaggat ttccccgagc
    19381 gcgcccggga agcgctgata atccagcgca tcgccagcgt cagccgacgc tccggagcga
    19441 cttccatagg cacctggtag gtagcgccgc caacacggcg ggggcgaact tccatgaccg
    19501 ggctgacgtt cttgagcgcc tgctcaaaaa cttccacgcc gtccttgccg gtcttggccg
    19561 caatcatatc gatggcgtcg tagaccaggc tggtcacagt gcttttcttg ccgtttaaca
    19621 taatgcggtt aatcatcatc tggatatgtg tgttgttgta acgcacatcc ggcaggattt
    19681 cccgtttctc tggtttaatt cttcttgaca tgattgctcc tcatcaaata caggcaaggc
    19741 agccgcgttc gccacttgag cgcgcctgcg attgccctta agcatccggg ctacttgccg
    19801 cgtttggcgc cgtacttaga gcgggcgcgc ttgcggtccg ccacaccagt cgtatccaaa
    19861 gtgccgcgca caatgtgata acgcacgcca ggcagatcct tcaccctgcc gccgcgcacg
    19921 agcaccacgg agtgctcctg cagctgatgg ccttcgccgg gaatataagc ggttacttcc
    19981 attccgttgg aaaggcgcac acgggcgatt ttacgcagcg cggagttcgg tttcttgggg
    20041 gtcatcgtac gcacgatggt gcatacgccg cgcttctgcg gggctccctt gggttggcgc
    20101 gtccgacgct gcttctctga gttaagcgtg tattgcagag cgggagattt gctcttctgg
    20161 tgcttggtct tgcgaccgtt gcgaactaat tgattaattg ttggcacttt gcctccgtaa
    20221 aaaataaaaa tcccaagtat tcattcatga aggccatcaa aactgcggat ggcctcctcg
    20281 tttgatccgt atcaccaggc acaagtgtac tctcagcctg gcaaagcgag gggtgattct
    20341 atcatgcccc ggaatgtgag tcaagcaatc cggtcggcag gaggccggag cgccgctcgt
    20401 tgtctttgcc gtagctgaca acttccccgt cttccatgat ggattcaacc gccaaaccaa
    20461 ggctttgcag ctctttcacc agcaccttga acgcagcggg ggtgcccgga gcttcaatcg
    20521 gcttgttggt cacaatcgcc tgataggtat tttcccttcc gaccacatca tccgacttga
    20581 ccgtcagcat ttcctgcagg gtgtgcgccg cgccgtaagc ttccagcgcc catacttcca
    20641 tttcgccgaa acgctgaccg ccaaactgcg ccttaccgcc cagcggctgc tgggttacca
    20701 ggctgtacgg gccggtcgcg cgggcgtgaa ctttgtcctc aaccaggtgg tgcagcttca
    20761 tgaaggtcat gtaacccaca gtcacaggct ggtcgtagcg gtcgccggtt ttgccgtcgc
    20821 gcagcacctg cttaccaata atcggaacgg gattgccggt ttccagcatg aggtcgtaag
    20881 cggctttgcg caggtcttcc atgctcaggt tcgggtccag ctcaagaccg agcgacttcg
    20941 cccacaggtt caggcaggcg cgcacggcgc gctcgtcctg ggcagtacgt tcgctgaccg
    21001 caatgttatc ttccgggaaa gcggtgatgc tggcagggtc gtaagcttcc gctttcagcc
    21061 actcatgcag cgcggtgcgg cgggcgagca ggcgattcga catcagttcc tgcacgtcga
    21121 tgccggtgct ggccagagcg tttgccaggt aaatcagccg ggcttcttca tcgtccttga
    21181 gatcctcagc ggaataggcg ccgaccctgt ccagccattc ccaggctttt tccccggtca
    21241 cgcgccaggc ttcgttaatc atccacgcgc gcgccagttc agcttcaatt tcctcttcgc
    21301 tcgctccgtc gaatacaggc gtgatggcgc ggaagccgag gctctccgcc gcccaaccca
    21361 agtgcgtttc gagcacctgt ccaatgttca tacggccggg aatacccagc gggttcagga
    21421 taatatccac cgggcgtccg tcttccagga agggcatgtt ttccaccggc acaaccatgg
    21481 agacgacgcc tttattacca tggcgccccg ccatcttgtc gccagccatt accttgcggc
    21541 gctgggcaac ggaaacgcgc accattttat ccacgccggc ggaaaggtcc gcgttctctt
    21601 cgcgcgtgaa tacctttaca tcgaccacga taccgcgttc cccgtgcggc atcttgaggg
    21661 acgtgtcttt cacatcgcgg gatttctcgc caaagatggc gcgcagcaga cgttcttccg
    21721 gggtcagttc tttctcgcct ttgggggtaa tcttccctac cagaatgctt tccggtccaa
    21781 cctcggcgcc gatacgaatg atgccgctct catccaggtt gcggactgcc tcgtcaccta
    21841 cattcggaat ttcccgggtg atttcttcgg gacccagttt ggtatcccgc gcttcgactt
    21901 cgtacttttc gatgtgcacg ctggtataac ggtcttcttc caccaggcgt tcggaaatga
    21961 ggatggcgtc ctcaaagttg tacccttccc aggctacgaa agcagccaaa acgttctgac
    22021 cgagggccag tttgccgtct tcggtggagg aggaatcaac gattacatct cccttgtgca
    22081 caatttgtcc tttgctcact gccggccgct gatcgatgca cgtggattgg ttggagcgct
    22141 ggtacttgcg caggttgtgc tcgcggatgg tgccgtcggc ctgccgcagc accagcttgt
    22201 caccggtaac tttgataact tcactttctt cttccgcgat gaccacctgg ccggaatccg
    22261 ccaccgcggc tgcttccatt ccggtcgaaa caatcggtac ttcggggcgc agcagcggga
    22321 cggcctgggt ctgcatgttc gagcccatca gggcgcggtt ggcatcgtca tgctccagga
    22381 aaggaatcag cgcggcgctg atgcccacca cctggttcgg agccacatcc atgtaatcga
    22441 tgaactcata atcgtaaatc gcgaaggtgg agtggtagcg gcaggagttg cggtgcagga
    22501 actcgtggta ttcgttcagc ggtgcgttcg cttgcgcgat gacatactta tcctccgcgt
    22561 ccgcggaaag gtagacgtat tcatcggtaa cgaatggacg cacccagact tccttcagcc
    22621 cgagcgtgct gattttcgcc gccatttcag gtgtcacgcg ggtttcagcc ttgaagagta
    22681 cctcattggt agccgggtcc acgatatcct tgcgcagcag atggttcacc agattggggt
    22741 cggagccttc cagatagtgc cgaactttgc ggtagggcgt ctcgataaaa ccgaactcgt
    22801 tcacggtggc atacgctgcc agacggccaa tcaggccgat gttcgggcct tcaggggttt
    22861 caatcgggca aatgcggccg tagtgggaga agtgcacgtc gcgcacgtca aaaccagcgc
    22921 gctcacggcg cagaccgccg gggccgagcg cggagacagt gcgcttgtgg cgcagctcgc
    22981 ttagggggtt ggtttcttcc atgaactgcg aaagttggct ggaaccgaag aattcgcgca
    23041 gggaagccac cagcgggcgg atgtttacca ggctgactgg cgaaaggctg cccccctgat
    23101 cgcggatgga catgcgctct ttgatgacgc gttccatgcg gcgcagaccc acgcgcagtt
    23161 tgttttgaat cagttcgccc acggtcttca cgcggcggtt gcccaaatgg tcgatgtcat
    23221 ccgcgttcgc cgtgccgttg ttaatcttaa tcatgtggcg gaccaggcgg acgacgtcgt
    23281 acttcgtaat catccggtga gagaccggga tatcatccac aaggtccagt ttctggttga
    23341 gcttataacg tcccacgcgt tccaggtcgt aatggcgggg gtcgaacagc tgctctacca
    23401 ggtgattgcg cgcgttatcg agcgtgggcg gctcgcccgg gcgcagcttc ttgaacagct
    23461 caatcagcgc ggcttcggaa atgctcaggc cgctgctaag gtcgtatgcg ggttcttcgt
    23521 cgaaggcggt ctcaatgaaa tgatggtcgg gattgttatc cacatcctta aagagctcca
    23581 tgatttcttc attgctgccg gtcttcagca gagaatcagg catgccgtca tccaccgctg
    23641 ccagcgcgcg caggaatacc gtaatcggta cggtgcgttt gcggttgaat ttcaggaaga
    23701 tgctgtcgtt cttgcgcgtt tcaaattcca tccacgcgcc gcggtccgga atcagtttgg
    23761 cggtagccag ttcgcgcgcc gtggaacggt ctacctctgc ttcaaaatag acgccggggg
    23821 agcggatgag ctgagagacc accacgcgtt cggtaccgtt aataatgaaa gttcccttgg
    23881 gggtcatttc cggaaagtcg cccaggaaaa tatcctgctt gaccttgtca ctgatatcgt
    23941 tgccttccaa gaggacggaa acatacagcg ggcaggcata ggtaaggtcg cgttcaaggc
    24001 actcttcgat ggagtttttg ggcgcgtcga accagaactt gagaccaaac tcccgtgaga
    24061 tgggggtatc gctggggaaa tgcaggcgga ggtcgttccc gtaagaaacg atgggggaaa
    24121 cttcgttgaa cagactcttc agaccctcat ccttgagtcg ttggaacgaa tccagttgga
    24181 cctggatgag ttgcggcagc tccaacccga tcgggatgcg agcgtacgtt tttggaggca
    24241 tcaaatcagt cattcacttc tcctaaacat caaatgcgtg ggcacacaaa ggcaaccacg
    24301 cagggattaa atcacagcgc aatttttcag tatattgcaa gttatctctt gagtcaatag
    24361 cgattgaatc aattgttcta agaaattgtt agtttaccac aaggatttat actccgcaag
    24421 gtccaattct atacggcttt gcgaagaggt tggaattctg gaatgtccgc gtgtcgagcc
    24481 tcggttttgc gtagggaggg ctgcgcactt caacgcgtca ttattgacgg cttgagaagc
    24541 gcaagccggc ctactttctt ttaatatcgc attcacagca agattcggat taccctacaa
    24601 cccatgctag gccttcaaag tgtgccaatt tggcatttgg agattcccac aaactattgt
    24661 ataattctca tttgcgtaaa tcgcgcagca ttttttgccg ggaacggagg tgatagccaa
    24721 tggcaagtgt aactttaaga gccaatgaat cgcaggattc attgctgaag cgctttcgta
    24781 agaaggtcgt gaagagcggt gttctcagca ccgtgcgccg caaacgctgg ttcatctcca
    24841 agagtgaaca gcggcgtatg gacaagaaaa aggctattcg tcgctcctac cggaaatcgt
    24901 acgaagcgta gccaacgtta gaaagaggtt ttatggcaaa agaagaagag aagattaccg
    24961 tcgacgggac aatcatcgaa gccctgccgg gcactcagtt ccgggtgcgg ctggaaaacg
    25021 gccacgaagt gatggcctac ctttccggtc gaatgcgccg ctattacatc cggatcctgc
    25081 tgggtgactc cgtgcgggtg gagatgtccc cgtacgacct tgagcgcgga cgcattgttt
    25141 accgccagcg ccgcccggga tcttctgacc tgcaagaatt ctaaacttgc ccccaaaaca
    25201 gccccctgat aatccttggg ggctgttttt ttcacgcgtc ccctctcaac cgtctatccc
    25261 atgccgcccc ccgaatccca ggactcttac ctccatatcc gccgcctgaa ccctaaccgg
    25321 gacctgaatg ccgtggcaga cctggtggag gaggcctttg aattgaggga tgaccccgaa
    25381 ggcgcctggg tcttgcgcca gatgcgcgct tttgcccggc gcgcccaaag ttacccggac
    25441 gttttcagcc cgcccggcgc tccggaaggc tttgtgtggg aagaagatgg aaaaatcatc
    25501 gggaacatca gcgtggtgaa atatttccag gcagaaaagc gccgggtcct gattgccaac
    25561 gtggctgtag cgcgggattc ccggcgcaga gggatagcca gcgcactcac ccgccacgcg
    25621 ctgcgctaca tcgggcagct gcctcgcagc gaagtctggc tgcaggtacg ttcggagaac
    25681 ctggctgcca tccaaatgta cacgcagttg ggatttgaat tcatctaccc gctctcccag
    25741 tggcgcagct cccctatccg cctgttcccc gtcccagccc gaaccagctt cccggactca
    25801 ctgcgggcgg ctgcccgcaa atccgcggat tggaaactcc agaagcagtg gctcgcgctg
    25861 aattaccccg cgcaaacccg ctggtacctc aacgtcaacc tggattggtt ttcaccctgg
    25921 agcttttttg taccggcttc ctggcagagc ctggcctccc tgcggcacta cagcctgcgg
    25981 gatggtccgc gcctgcgcgg cgttctcacc tggcaaaaaa cgaggctctc ctcagaccag
    26041 ctttggctgg ctctgcctga ggggccgcag gaagatttgc acgcggaaat gctcctcgac
    26101 gctctgcaga aaatagaatc tccggaacgc tccttattgc tggagtatcc gcgcggccgt
    26161 gcgcagatgg gaattcagag cgccggcttc acgctcgcac gaaacctgga ttggatgaaa
    26221 ctgaaaaatt cctgaatccc accaaaggta agaagaaggg ctgccccgcg cggagcagcc
    26281 ctttcagctg gtttagagtt cctcgtccac gatggtttcg atgaggtcaa ggaattcctg
    26341 ccattcttcc ttgaaaaagt gcagggtcac attgttgagc tcaaggtgat aagtggattc
    26401 gttatccggc tcatccgcgc gccaggcaag gtaattctcg gtttcagcaa tagtcgtggt
    26461 gacaggttct ttctcgttgt ccatgtttag ctccttttga tttctggttc tcattatctc
    26521 attaagcgcc gctctgatgc aagttaaagg gctgttcgcg cgctagatgc gcttttcggg
    26581 tcttttcgaa gggggagttc cccgattaat tactttgtgc cagcctttcg caatggacac
    26641 acgcgcctgc ttgaaccaca gcggaatttt cttttttaaa tagcggaaaa aacgggtaag
    26701 cccaagctct tttcgccatc tgctgtaaga aatgccttcc cacgggtcct ggtcattaag
    26761 cttgtgaaat acataatgcg tgaggagctt gaccgtggca aacaccggcg ccgccaggat
    26821 gacgccgatg atgcccagca gctgagagcc aatcagcgcc gtaatcagca ccgccgccgg
    26881 atgcactttg agcgcgttgc ccatcacctt cggtgtcatc acattgtcca ggatgttatc
    26941 cactaccagc gccacaccca ccaccacagc catataaacg atgggcagca ggccaaaagg
    27001 ggtcgggcgg gtaatcagcg ccacaatcgc aaaagtcagc caactgaccc aggcgccgac
    27061 gtatgggacg aagcgcccca aacctgcgat gagcgccaga cccagcgcat aaggcatctg
    27121 cagcccgctt agcaggatgg agtaaataaa aatggcactg ccgaccacta aaaattcgcc
    27181 gcggatgaaa gcgttccaga tgttgctaat ttctttgccc atgcgctgaa tatctttttc
    27241 atatccaggc aggccaaaac tgattttctg cttcttgacc ccgtccgtct cggaggttaa
    27301 gaagtacgaa atcagataca tcatgaacag gcggaaaacc aggttcgcgc cgccggagag
    27361 caccttgccg atcagactgc ccgcctcccc cagaatggac tgcacctgcc ccaccagcag
    27421 gtcgctcaga taggtcgcgg tgagcttcgg cacaaaaaag ctgaaaggcc cccacacgac
    27481 ttcctggttg gaccattgtt ccagattttt cacaagcccg ctcagcgcac tgctcaggag
    27541 gtcgatcaag ttttgcagct ggccgaaaag cgtgatgccg ccccaggtga gcaagcccaa
    27601 aaggataagc gccatcaacg cgtacaccag cgtcgtcgcc aggcgccaac tcaacttgag
    27661 gtgtttttgg atgaaggtga tcatcggggt gaacaggaaa acaatcagca atgctgtcag
    27721 tatcatgctg agataattct ggaacttgat aatcagccca aatacaagca gaataccggc
    27781 aaagctgatc gccagcttgg tatttttgga ccagcgcggt gaaacagctt tttccgtgtg
    27841 ggtctgcgcg ccttctttta gcggttcggg gtctgtaggc tcagccatgt ctgaatttta
    27901 actggtatca ggggaatatg caaaa
//
LOCUS       DAOQAD010000014        27216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_1743_length_27216_cov_6.748279, whole genome shotgun sequence.
ACCESSION   DAOQAD010000014 DAOQAD010000000
VERSION     DAOQAD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 27216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27216
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1743_length_27216_cov_6.748279"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..1992
                     /locus_tag="PKL60_02360"
     CDS             268..1992
                     /locus_tag="PKL60_02360"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNW95011.1"
                     /translation="MTEAYSINDNFDELNLRPELAQAVNTLGYTQPTPIQRGIIPLMM
                     AGYDVTGQAQTGTGKTAAFAIPILQRLDPAARLPQALVVAPTRELALQVAEMTQALGQ
                     YLEVKVLAIYGGTAYSEQLAGLRRGAQIVVGTPGRLLDLAKRGRLDLSAVRFVVLDEA
                     DEMLSMGFIEDVESLLAQTPGERQTALFSATLPKRILQLAENYLREPKTVRIEAEQVT
                     VSATEQRFYLVNEFDKLAVLTRLFEVENISTALIFTRTRARTGELVNELNLRGFPAEA
                     LSGDMSQDARERTMARFRSGNVKVLVATDVAARGLDVEGISHVFNFDLPDEAELFVHR
                     VGRTGRAGRTGTAISLVTPSERRLLREIEHFTKQEMRACSIPTPEEIQAKRLNDLMTK
                     IMMWLKRGRAKEERALVDGLVANGADPLELAAVALKIARAEEKQRPIASVSPLPEKAV
                     RPERRPRAGAGFAGKTAEDRSSAPRRTHEERSAREPRRDSADHPREAGMVRMVLGLGR
                     QDGIRPGDVVGAIAFHADIPGSSLGKIIIQSDRTMVDVPEEYVGKVLAGNGRYIIRKQ
                     EFNLLAMN"
     gene            complement(2048..2656)
                     /locus_tag="PKL60_02365"
     CDS             complement(2048..2656)
                     /locus_tag="PKL60_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95012.1"
                     /translation="MKYKVVVPMNPNLQAFETGRQSLQVNFPQHCVYCNKPVETYQVI
                     DLSGGKTVGKRTASFSAQLHVPYCLDHSIVFSRYKNLLGAIGLPVFLIVFIGWFFLFK
                     PLTAFGNTLPSGLNFLLIPLIIPCIGNLILGFLAFILVHLLLLLFEPKFRQIPFVTEE
                     GGLGLTVNMKTSMYAINSLDFTFTNRDYAAEFAAANHVSMTG"
     gene            2893..4098
                     /locus_tag="PKL60_02370"
     CDS             2893..4098
                     /locus_tag="PKL60_02370"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNW95013.1"
                     /translation="MEIAVIGAGASGIFAALAAAQPGKRVTLFDHNPEIGKKLLVTGS
                     SRCNISNTMVSPERYACADEGWMRAFFQAYPPARLLNLLDELGIPTYRTADGWYYPLS
                     NSAHSVVEILRANLLAREVEDSLSTQVVDLRRAGGGFALRFPSGEPARERFFDKVVLA
                     AGGKAYPALGSRGELFPALTRLGHRVVPLVPALGPLVVASGKFQALQGLRFDVGTAVY
                     QGGQRLGSSFGNLIFTAKGLNGPGVMNISHLAARGGEGLRLELDFLAPFHAELSEDAL
                     RRTNSLGPLVFLNRFFPPKASRFFLDYAGIGAGDPDGLQKLLAALRSVSLPILGTRSF
                     DESQLSAGGVPVTEASPETCESARVPGLYLVGETLDVVGPCGGYNLHFAFTSGTLAGL
                     HLAGLAERT"
     gene            4177..5559
                     /locus_tag="PKL60_02375"
     CDS             4177..5559
                     /locus_tag="PKL60_02375"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNW95014.1"
                     /translation="MPIPFIEPFRIKVVEKLKVTTPEERADALAKAHNNLFAVPAEDV
                     FVDLLTDSGTGAMSDAQWAGIMRGDESYAGARSFAHFESAVQEVLGFPYVLPAHQGRA
                     AEGMLFQTLVKAGQIVPNNRHFDTTQANLLVLGADPVDLTVPEGDDPQADLPFKGNMD
                     LERLEDLIRRVGADRIPLGMTTITNNSAAGQPVSMENIRRTSEIYHRYGIPFFIDACR
                     FAENAWFIKLRDPQYREVPVTQIVKEIFSYADGCTMSAKKDAIVNIGGFIAFRDPGLK
                     QRIAEHLIVHEGFVTYGGLAGRDLEALAVGLYEGIDEEYLAYRESHTRYLGKVLSEAG
                     LPVYLPTGGHAVYVDAGKALPQIPAAQFPGVALANALYLEGGVRTTEIGSLMFSRPDP
                     ESGKMIYAPMELVRFAIPRRVYTRSHLDYVGEAAAALVRRAGNLRGLRISWAPPVLRH
                     FTAHLEETLE"
     gene            5770..7125
                     /locus_tag="PKL60_02380"
     CDS             5770..7125
                     /locus_tag="PKL60_02380"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNW95015.1"
                     /translation="MRVLIIGTGYVGLNTGVVLSYIGHSVTCLDTNAEKINLLRSGKT
                     PFYEPHLDEMLAEVGPKMRFTTSYAEADLSTMDVVFIAVGTPSLPDGNADLSYVRQAV
                     EEIAQEMDDHFLVVVNKSTVPIGSGNWVGSLIKESYEKRVGAKADGRFSVVSNPEFLR
                     QGAALYDSFYPDRVVVGSENSHGIQRLSELFAPILEQNFRAPTCLPRPEGLKQVPLLV
                     CDLTSAEVIKYAANAFLALKISYINEIARLAQRVGADATLIARGIGLDSRIGPRFLNP
                     GIGWGGSCFGKDTAALVATARDYQLEMPIVQAARDVNYRQRAWVVDALQEGLKILKGR
                     HVTLLGFSFKPDTDDLRDAPSLDIARLLVQRGAIVRAHDPVALKNAERLYPELGVRYC
                     QDVYEALHGAEAIVLVTEWPLYRELDWEKIPPVLVVDGRNFLDREKLKNLGFRVLGVG
                     R"
     gene            7323..9008
                     /gene="hcp"
                     /locus_tag="PKL60_02385"
                     /gene_synonym="priS"
     CDS             7323..9008
                     /gene="hcp"
                     /locus_tag="PKL60_02385"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNW95016.1"
                     /translation="MPEMFCYQCQETLQNTGCVRRGGVCGKSAEVSNLQDLLIYLLKG
                     ISFWGTRGRDMGVVNEEADLFVAQALFATITNANFDPQRFVELIKKAILLRDDLRQRA
                     QAACYEQHGGDPCRSSGPDWAGWHPADYTLETLLEKARTVGILADAELDPDVRSLREL
                     IVYGLKGMAAYADHAYVAGGRDGSVLAFLQEALEATTRKDIGVNELLAVVQRTGEFGL
                     KAMALLDSANTNRYGHPEPTLVNLGVRPGPGILVSGHDLVDLDELLQQTEGTGINVYT
                     HGEMLPTNAYPGLKKYAHLAGNYGGSWWKQSREFESFNGAILMTTNCITPVRDSYRDR
                     IFTTGMAGFSGVPHIADREPGRQKDFSALIARARNCESPLELENGTIPIGFAHHTVLS
                     VADKVIEAVKSGAIRRFVVMGGCDGRQKERDYFSQVAQKLPKDTIILTAGCAKYRYNK
                     LGLGEIAGIPRVLDAGQCNDSYSLIVIAQALAQAFKVSVNELPLSFDIAWYEQKAVIV
                     LLALLALGVRKIRLGPTLPAFLSQNVVDVLVKAYELQPIADAQEDVQAILAGH"
     gene            10092..11147
                     /locus_tag="PKL60_02390"
     CDS             10092..11147
                     /locus_tag="PKL60_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNW95017.1"
                     /translation="MAGKSKSRSPLNLPDLKTLKPRDPAAPSPAQAYLNALSPSGRRS
                     QHTALNNLAYILSGGALNDALTFPWELLRYEHTSGLAAYMVDIGYARSSVNKHLVALR
                     RVLAESYQLGLYADHNDYYRAAGVKSLRSETLPRGRTLRMEELRALIQTCLKDAKNPT
                     LGVRDAAVITVLYAAAVRRQEIVDLNLADLDLKEGKLRIPGKGSKKRQVYLQADAVRA
                     LQNWLALRGRQLGPLFTRVTKSGRILLKRLTPQAVYYLLKHRQAQAGLDDFTPHDLRR
                     TSITDLLSAQVDVLTVSAIAGHASADTTRRYDRRSDENKKAAAQKLPSPFADPPSPTE
                     NPQQEKGVHPSDGNPQE"
     gene            complement(11199..11510)
                     /locus_tag="PKL60_02395"
     CDS             complement(11199..11510)
                     /locus_tag="PKL60_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95018.1"
                     /translation="MIPTAFASSAGTPKGAPKGTRPAVETSQPASGEAAQTPSTLSDA
                     EMEALIQQKLQGSPHVLPFILGQKRTADEWSKILDRMIGYGAKINAQEKQLIIGWLVS
                     R"
     gene            complement(11687..12682)
                     /locus_tag="PKL60_02400"
     CDS             complement(11687..12682)
                     /locus_tag="PKL60_02400"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HNW95019.1"
                     /translation="MLFVDNQNLHDPALNLALEEYLIRNIAPLEELLLFYINEPSIII
                     GRHQNTFAEINVEYVREQGLDVVRRLSGGGAVYHDLGNLNFSFIAPNLGGSFANFQKF
                     TAPVIQVLRDLGVQAELSGRNDILAEGRKISGNAQYVSGARMVSHGTLLYNSDLEAIG
                     KALRVKASKIESKGIKSVRSRVANITEFLSEPVDILSFRRQILSGVFAQQTAADIPEY
                     QLTPADWEAVRQLARERYQSWDWNYGSSPAFNLRRSVRYAGGEIEVYLVVQKGMLHSI
                     KFIGDFFGQRDVAELEERLAGCPYLPDALGERLTAFEVNQYFAGFSLAELLSLLY"
     gene            12886..13293
                     /locus_tag="PKL60_02405"
     CDS             12886..13293
                     /locus_tag="PKL60_02405"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNW95020.1"
                     /translation="MSLENETISSIPHGSVTEAEILLLHQQVCYALGDPTRIMLLYLL
                     REGPQSVGELTERMKLPQSTVSRHLRVLRERSLVLVEKRGTSNLYTITDTRITDALDL
                     LRQVLNQQLSEQAKLTAAIQCSYPPNQDFSDKE"
     gene            13297..15759
                     /locus_tag="PKL60_02410"
     CDS             13297..15759
                     /locus_tag="PKL60_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW95021.1"
                     /translation="MKVVIVGGVAGGASTAARLRRVDESAEIIMLEKGPYVSFANCGL
                     PYHIGGVIQDRDQLLVVTPETLRDMLNIEARTNSEVRSIDRQNKTVVVKELTTGREYQ
                     EPYDKLVLATGAVPIVPPLPGVDLPGVYVLHNIPEMDAIESCLSEACPKSVIVVGGGF
                     IGLEIAENLAEKGLQVTVVEMLEQVMAPVDFELAAMVHHHLAFHNIGLFLGDGLKSIE
                     KTGPDGSLQVNLASGKSLPTDMVVLAIGVRPETSLAKAAGLELGVRGTIATNDQMQTS
                     DPDIYAVGDDAQLTHNILEQPTFLALAGPASREGRTAADAIAGRSTKFPGVQGTSVVK
                     VFDLTVASTGLNQRQLEQAALPFESVIIHANSHASYYPGAAQMALKLLFSPQDGRIYG
                     AQAVGPDGVDKRIDVIATAIKGNLSVYDLEELELCYAPPFGSSRDPVNIAGFAAGNIL
                     RGDAPVLHWNEVDGLDRSTWFILDARLPEEGTLGSIAGSRNIPMQQLRQRLGELPRDR
                     KILVYCASGQRSYFAVRMLRQNGFDAYNLSGGYKTYAYAVSPQSHTPEPETKTMTLPS
                     TDAIPQAPAGSEYQLDACGLQCPGPILKLYNKVKEMEDGDVVTVKATDYGFANDVGSW
                     TKATGNTLLNLDSAGGVITARVQKGQHAEEAAAASGTGKNGKDMTMIVFSGDLDKAIA
                     AFIIANGFASMGQRATLFFTFWGLNLLRKDNPPAVKKNLVERMFGWMMPRGAKKAVLS
                     QMHMLGLGTGMIKGIMKQKNIDSLPVLIKTAQQNGVRLLACQMTMDLMGIKREELIDG
                     VEVAGVATMAADASTTNTHFFI"
     gene            complement(15938..16978)
                     /locus_tag="PKL60_02415"
     CDS             complement(15938..16978)
                     /locus_tag="PKL60_02415"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNW95022.1"
                     /translation="MTASILEREILEQASVLRRLLGRGVDEARELINPLRGRFHYILI
                     AARGSSDNAARYAQYLFQIVNHIPVALATPSVFSVYRKPPLLEGALVLGISQSGKSPD
                     VISVLDAARAQKRPTIAITNIPGSPLEASADCTLALGAGPELSVAATKTYTSSLAAVA
                     LISAALSGEKRRLDELARLPDQAEAALALSLELTPRMERFRFMQHGAVIGRGYNYSTA
                     FEIALKIKELTGVTTVPYSSADFLHGPVASLHKGYPLLAVAPSGVMYRDTAALIRKAR
                     RLGAELVVISDREEALGMAQLALPLAAGTPEWLSPIPAVIPGQVLAWQMALSRGLDPD
                     HPRGLSKITETY"
     gene            complement(16997..17548)
                     /locus_tag="PKL60_02420"
     CDS             complement(16997..17548)
                     /locus_tag="PKL60_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNW95023.1"
                     /translation="MSERVTFFIDGTNLYFGLIKDLKRLDLDFQKLVQKLLNGRTLSR
                     VYYYAVAPTEEQANLVTEIERYKRQMKFLEALRRTPYFEVVLGRLVLHNSSYIEKGVD
                     VAIAVDMLDLQSTYETAVLISGDSDFCRAVQVVKRGGKHVENATTRTISSRELQQACD
                     VFYLLDDEYLKDCWRYKTSGFNS"
     gene            complement(18026..19411)
                     /locus_tag="PKL60_02425"
     CDS             complement(18026..19411)
                     /locus_tag="PKL60_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW95024.1"
                     /translation="MANRLKSFLTGLLTIRPEDEVPHNVAKNFRHNVTMGSLDLMFFI
                     FGDGFGSITTIMPVFAATLTKSPLLIGLIPAIANFGWYMPQMFLSNYVSGLKQKHPFA
                     VKMAVVERVPYVFFPLLALLVPSIPKTTALLWLFILITWRGLASGLIALPWQEVQASV
                     IPITHRARFYGVSRVLAQFMGILSSVLATIFLSRLPYPQNYALCFATAVVAQWFSFYF
                     YSRIREPVNTELETASADPASSAGARMEQEKTKPARKIIDLELFGAILKRDKNFRYYL
                     VGRSVIFLGAMASGFLAVYGIQRFNLSDSRAAVFTALLYFSGIIGYTLGGMLGDKVGP
                     KRIVVASVLIWAVGMLIAILARSEWAYYLVFLMFGLNSAGMVLGDSILVMELGEEKLR
                     PTYLGMARSLTGIFVLVAPVFAGALVEAFGYNVMFTVCLALTLLGAAIISRVKDLPRR
                     ASRLKSEQTSV"
     gene            complement(19515..21353)
                     /locus_tag="PKL60_02430"
     CDS             complement(19515..21353)
                     /locus_tag="PKL60_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNW95025.1"
                     /translation="MTTEIPESLRDHSFGDLDTPSIGAAFLMDRALGVHHFGRKSPLV
                     PKPGDAVTLYAYTSSGCPVGDLVLRYSTNDWRDYAELPFQPEGQNWDTLAWAWITGWR
                     ALLPPQPAGTLLRYQVYARLPEREAGQTSPGLLFADTQSADARSADQFAIWYGADSVP
                     AWSRSARIYQIFVDRFNPGQGRDWLQQEDLARPFGGTLRGVIEKLNYIHDLGFNAIWL
                     TPIFASPSHHGYDTSDYLRIEPRFGTLADFEELITRAHALGLRVILDFVANHVSNQHP
                     ALLEALKSASSPFTAWFKWVKWPEYQGYFNLRSMPELNLAHGSPAREHLFDAARYWLR
                     LGADGYRLDYAIGPGLDFWVEFRKICREVNPEVWTFGEVTRPADQQLSFAGGMDGTLD
                     FLSCQALRESFAFRSKPISGFAGFLQASQAYFPREFSRPAFLDNHDMNRFLFAAGGSQ
                     RRLKLALSWLYLLPGQPLVYFGSESALSQALSIHDPAAMGFDQSRLAMNWGEVDQHSE
                     LADHIRALSAFRERFPGLVDADWLLLALDDERETALWRVRLADTSFFAALNRSEEPRT
                     IPVPQDCLGGDQSVFDMLTGEHLPAVFGRVHIPAESVLILANKTGG"
     gene            complement(21542..22696)
                     /locus_tag="PKL60_02435"
     CDS             complement(21542..22696)
                     /locus_tag="PKL60_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNW95026.1"
                     /translation="MAENPVTKKAAKTPFKIKELGFLVFIGLMVLLPFVMNLITGTGL
                     NEGVTKFWQGQLIAFFIMAVFAMSYDLLIGFGGILSFGHAASFGGGAYAFGLLMKHWA
                     PNFIKNNPIVLGNTDLTMVVVLLVVLLVVLLVSVAIGLLFAVTSMRLKGTYFAMITLA
                     LSTAFYIIIKSTDLHKWTGADEGLHGIPLPVWLNPTQNRLTVYFITLAFLFVSYLLLR
                     RFVNSPTGRIIVANRENEDRMRMIGYNPVTYRTIAFVVASVFAGLAGMFYSIWNMSAT
                     PTMISVVTTVNALIMTIMGGMGTLIGPIFGAAISQIIQQFFYTWFGARWPLIFGALFI
                     LIVMFLPYGIVGTWRFQKANLHEGWKRFLKLFASKADAAEAENFQKPPEA"
     gene            complement(22703..24196)
                     /locus_tag="PKL60_02440"
     CDS             complement(22703..24196)
                     /locus_tag="PKL60_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95027.1"
                     /translation="MNPKSKNILLGLLIAGLLVAVVVGVYTYAGQRAFISTLFSGLTL
                     GSLFFMVAAGLTLIFGLMDVLNFAHGSMFMLGAYIGWQFYTNPTFIFGMAPFLLAFGA
                     GVMLTPIIKPYLIAWKVSEKMQKALPKIAYVLALLALVFAFVGFDILSVADTAMVAAT
                     TTTIGNPLAEISAQEPLSSYWLRPLLMFVAGLLIAVASSRPGDKTQFAPREPIGKKLL
                     MPAALLVLTVLSTLVRESASEALLLMNGNLRFVLSILLAVAFGFVFGAVIEITLIRPL
                     YVRPTFILLMTLGLSYVIRELVQYLWDPLTYQMSRPPLFAQPGKATSIAEWLQKGSAT
                     IDIYGATVPTYRLFIIALGIVMFVVVLFVMTRTRLGMTIRAGVQDPQMVEAMGINVKA
                     VFTLVFAMGVALAALGGIGAAPFLPVQPLMGDTYQTQGLITVVIGGMGSYVGAFVGAM
                     ILGMARAFGDYFALKLSLSPAIAEASTVIIMVIVLLTKPQGLFGKKE"
     gene            complement(24180..24947)
                     /locus_tag="PKL60_02445"
     CDS             complement(24180..24947)
                     /locus_tag="PKL60_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95028.1"
                     /translation="MSAQIPANDLLVVDRIETFIGQFHILENVSVRVPKGSITVLLGR
                     NGAGKTTTLKSIIGLTPPREGKITFDGLEIQGKQAFNIANLGMGYVPENRAIFRALSV
                     LENLKLAERKKGDFERKSDFIFELFPDLKRLYKLPGNHLSGGQQQMLAVARALVPDNK
                     LLLIDEPSEGLAPILIQQMMEAIRQLSQTTTILLVEQNFIMASQLAERYTIIDDGKSV
                     QEGLMEDLVEDTDLIHHYLGASIKTRKELEMEHESEK"
     gene            complement(24944..25714)
                     /locus_tag="PKL60_02450"
     CDS             complement(24944..25714)
                     /locus_tag="PKL60_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95029.1"
                     /translation="MANEVLIEAVNLSKAFGGLIAVDNVSLQVREHTIHAIIGPNGAG
                     KTTLFNLLSGVMPPTSGHVIFKGNDITQVSPQRRAHLGIGRSYQITNIFPNLTVLENI
                     RLAAQAMGKDNFKMLHLADNFRKYIDAAEEVIGVVGLEGREWVLARNLPHGEKRKLEL
                     GIMIACSPELLLFDEPTAGMSSEQVPDLINIIKRVVNHGDRTAILVEHRMDMVMSISD
                     VITVMNQGRVLAEGSPHEIASNLQVQTAYLGDLYGELA"
     gene            complement(25916..>27216)
                     /locus_tag="PKL60_02455"
     CDS             complement(25916..>27216)
                     /locus_tag="PKL60_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNW95030.1"
                     /translation="DSGYYTADQFTGLEEGAAAPTEELGPVLKIGQIGMMSGSMALYG
                     LQQQRGFELGLEYMSGGKKDDQGRWIIANRPVEVIVKDDEGNAEKAVALALELIEKDG
                     VELLQGPVSSASAAALTTVALENKIILMVDPAASYFTTGVYFNPYVFRTARTSFDDTL
                     IIAKYLVENVGPTFAHIGIDNAFGQASGAALKYSVEKFGGELIADIYAPFETTDFTPY
                     IQQAMDSGAKNLFLTWSGTGYVTLFQGLADMGALDVMTVGTGFGDNASFTAVFGEDVL
                     GTVGLNVYHYTAADNAVNNWLVEHHVAKYKDAPDGAINAYPDLFTAGGMASAIALATA
                     LEKTGGDTSGEAMIPALEGLTFEGPKGTYHIRPEDHVCEQPMNILKLVNLDPDLDDDG
                     INEYRFFETIYVSKYDELGVPCTLEGEYASRCGDLPTLGQ"
BASE COUNT         6030 a         7640 c         7986 g         5560 t
ORIGIN      
        1 ccccttgcca gcgcgggaaa tgtgatatac tctggcggct ctcatggtga gagcttttct
       61 ttgggtgtgt tttggacgtt ccaatttcag ccctgggtta gccccgggca ttttgttggg
      121 aggcgcggca ccgcgaggag ccgcccggct gtaataaaac gttttgcgga atcatcaaga
      181 catccccctg agtgaaaatg aatttgcggt aatcagcccc tgggtttcag ggcgctcttc
      241 accgcgcaag caggaacagg agttttgatg accgaagcgt acagcataaa cgataatttt
      301 gatgaattaa atctgcgccc cgaattggcg caggcagtta acaccctcgg ttatacccaa
      361 ccgacgccca ttcagcgcgg gattatcccc ctgatgatgg ccgggtatga cgtcaccggg
      421 caagcccaaa ccggcaccgg caagaccgcc gcgtttgcca ttcccatcct gcagcgcctt
      481 gacccggccg ccaggctgcc gcaggcactg gtcgtcgcgc cgacgcgcga actggctttg
      541 caggtggcag aaatgaccca ggctctgggt caataccttg aggtgaaagt actcgccatt
      601 tacggcggca ccgcctacag cgagcagttg gctggtttgc gcaggggtgc gcaaattgtg
      661 gtcgggacgc ccgggcgcct gttggacctg gcaaagcgcg gacgcctgga cctgagcgcc
      721 gtgcgttttg tggttctgga cgaggctgac gaaatgctct cgatgggctt catcgaggac
      781 gtggaaagtt tgctggcgca aaccccgggt gaacgtcaga cagccctctt ttccgccacg
      841 ctgcccaaac gcatcctgca attggccgaa aactacctgc gcgagcccaa aactgtgcgc
      901 atcgaagccg agcaggtgac cgtatccgcg acggaacagc gcttttacct cgtgaacgag
      961 ttcgacaagc tggcggtgct cacgcggctt tttgaagtcg agaatatcag caccgcgctg
     1021 attttcacgc gcacccgcgc ccgcaccggc gagctggtga acgagctgaa cctgcgcggc
     1081 ttcccagcgg aagcgctcag cggtgatatg agccaggacg cgcgcgagcg cactatggca
     1141 cgctttcgca gcgggaatgt caaagtcctt gtggcgacag acgttgccgc gcgcgggctg
     1201 gacgtggaag gcatctcgca cgtcttcaac tttgacctgc cggatgaagc cgagctgttc
     1261 gtgcaccgcg tgggcaggac cggcagagcc gggcgcacag gcaccgcgat ttcgctggtg
     1321 accccctccg aacgccgcct gctgcgggaa attgaacact tcaccaagca agaaatgcgg
     1381 gcttgcagca tcccgactcc ggaagaaata caggcaaaac gtctgaacga cttgatgacc
     1441 aaaatcatga tgtggctcaa gcgcggcaga gccaaggaag aacgcgcgct ggtggacggc
     1501 ctggtcgcca acggcgcaga cccgctggag ctggctgccg tggcgctgaa gatcgcccgt
     1561 gcagaagaaa agcagcgccc gattgcttcc gtcagcccac tgcccgaaaa agcggttcga
     1621 ccggagcgcc gcccgcgcgc cggggctgga ttcgcgggca agactgccga agacagaagc
     1681 tctgcgccgc gccgcacgca cgaagagcgc tctgcgagag aaccgcggcg ggacagcgca
     1741 gaccatccgc gcgaagccgg gatggtgcgc atggtgcttg gcctgggacg tcaggacggc
     1801 atccgacccg gcgatgtggt tggggcgatt gctttccacg cggatatccc cggctccagc
     1861 ctggggaaaa tcatcatcca gagcgaccgc accatggtcg atgtccccga ggagtacgtc
     1921 ggcaaagtgc tggctggcaa tgggcgttac atcatacgca aacaggaatt taacctgctg
     1981 gcgatgaatt gattttctga gagcggaagg agcgcggacc cgactggtcc gcgctttttt
     2041 caactgttta cccggtcatg ctcacgtgat tagcggcggc gaattccgcg gcgtagtccc
     2101 tgttcgtgaa agtgaaatcc agtgaattga tcgcatacat gctggttttc atattcacgg
     2161 ttaagcccag tccgccttct tcggtcacga acggaatttg tcggaatttg ggttcgaaca
     2221 gaagcagcag gagatgcaca agaatgaacg ccaggaaacc gaggatcagg tttccgatgc
     2281 aagggataat tagcgggatg agcaggaagt tcagcccgga cgggagcgtg ttcccgaatg
     2341 ccgtcagcgg cttgaagaga aagaaccaac cgataaagac gatcaggaag acgggtagtc
     2401 cgatcgcgcc gagcagattt ttatatctgc tgaacacgat cgaatggtca aggcagtagg
     2461 gcacgtgcag ttgggcggaa aacgaggcag tacgtttgcc aacggttttt ccacccgaga
     2521 gatcgataac ctggtatgtt tccacgggct tgttgcaata gacacagtgc tgggggaagt
     2581 ttacctgcaa gctttgcctg ccggtctcga atgcttgcag gtttgggttc atagggacaa
     2641 cgactttata tttcatgggg acctcctttc atgaaatcag gtgttggtga ggcatctcgc
     2701 gcgcaagtcg ggagaccccg tcatcttgat gccctaaagc cgtgggcgag aagcaaggat
     2761 actgggagcc tcagcgattg ccgtatggaa aattggattg gacacttcca ttatgcgtat
     2821 ttttgagtcc aacgtcaacc tggcttcaga gcctggcgtg gggtagggcg ctttgcgttg
     2881 tataatcggg ttatggagat tgcggttatc ggagcagggg cgtcggggat attcgcggca
     2941 ttggcagcgg cacaacccgg taagcgcgtc acgcttttcg accataaccc tgaaatcggg
     3001 aaaaagctgc tggtcaccgg cagcagccgc tgcaacatca gcaacacgat ggtaagcccg
     3061 gagcggtatg cttgcgccga tgagggttgg atgcgcgctt ttttccaggc ctacccgccc
     3121 gcgcgtctgc tgaacctgct ggacgagctc ggcatcccga cctaccgcac cgcggatggc
     3181 tggtattatc cgctctccaa ttcagcacac tctgtggtgg aaattctgcg cgccaacctg
     3241 ctcgcccgtg aggtggaaga ttcgctttct acgcaggtgg tcgatttgcg ccgcgcgggg
     3301 ggcggcttcg cgctgcggtt tccctccggc gagccggcca gagagcgttt cttcgacaag
     3361 gttgtgctgg cggccggcgg caaagcctac ccggctttgg gttcgcgcgg tgaattgttt
     3421 ccagcgctca cgcgccttgg acaccgtgtg gttccgctcg tccccgcgct ggggccgctg
     3481 gtggtagcgt ctggcaaatt ccaggctctg caggggctgc gctttgatgt gggtacggct
     3541 gtctatcaag gcggccagcg cctgggctcc tcgttcggca atttgatttt caccgctaag
     3601 ggcctgaacg gcccgggtgt gatgaacatc tcgcatctgg cagcgcgcgg cggggaaggt
     3661 ctgcgtctgg agttggattt tctcgcccca ttccatgcgg aactttctga ggacgcgctc
     3721 cgccgcacca acagcctggg cccgctggtc ttcctgaacc gcttttttcc gcccaaagcc
     3781 agccgcttct ttctggacta cgccggtatc ggggcggggg atccggacgg gctgcaaaag
     3841 ttgttggccg cgctgcgcag tgtctcgctg ccaatcctgg gaaccagaag ctttgacgag
     3901 agtcagttga gcgcgggcgg ggtgcctgtg acggaagcct ccccggagac ctgtgagtcc
     3961 gcccgggtcc ccggtttata tctggtcggg gagacactgg acgtcgtcgg gccctgcggc
     4021 ggctacaacc tgcactttgc gtttacttcc ggcacgctgg cgggccttca tcttgccgga
     4081 ctggctgaga ggacttaatg ccctgttgag cgcgcttggc agatgtgctg tctacgcgtg
     4141 ctttgcaagc aaaccgaatg acttaaagga gagaccatgc ccatcccgtt tatcgaacca
     4201 tttcgtatca aagttgttga gaagttgaag gttactaccc ctgaggagcg cgcggacgcg
     4261 ctggcgaaag cgcacaacaa cctgttcgcc gtgccagcgg aagacgtgtt cgtggacttg
     4321 ttgaccgaca gcggcaccgg cgcgatgagt gatgcgcagt gggcgggaat aatgcgcggg
     4381 gatgagtctt atgccggcgc gcgcagcttc gcgcattttg aaagcgcggt gcaggaagtg
     4441 ctcggctttc cttacgtctt gccagcccat caaggccggg cggcggaagg catgctgttc
     4501 cagacgctgg tcaaagccgg ccagatagtg cctaacaacc gccattttga cacgacccag
     4561 gcgaacctgc tcgtgcttgg cgcggatccg gttgacttga ccgtccctga aggcgatgac
     4621 ccccaagccg acctgccttt caagggcaac atggacctgg aaaggctgga ggacctcatc
     4681 cggcgggttg gagcagaccg catcccgctc ggaatgacca ccatcaccaa caattccgcc
     4741 gccgggcagc ccgtctcgat ggagaatatc cgccggacca gcgagatcta tcaccgctac
     4801 gggattccat tctttatcga cgcgtgccgc ttcgcggaaa acgcctggtt tatcaagctg
     4861 cgcgacccgc agtatcgtga agttcccgtg acgcaaatcg tcaaggaaat tttctcgtac
     4921 gcggacggat gcacgatgag cgccaagaaa gacgcgattg tcaacatcgg aggattcatt
     4981 gccttccggg acccaggcct aaagcagcgt atcgccgaac acctgattgt gcacgaaggt
     5041 tttgttacct acggtgggtt ggcagggcgc gacctggaag ccctggcggt cggtttgtac
     5101 gagggcattg acgaagaata tctggcttac cgcgaaagcc atacgcgcta cctgggcaag
     5161 gtgttaagcg aagccgggct gccggtttac ctgcccaccg gcgggcacgc cgtatacgtg
     5221 gatgccggca aagcgctgcc gcaaattccc gcggcacaat tccccggggt cgcgctggca
     5281 aacgcgctgt acctggaagg tggtgtgcgc accactgaaa tcggcagcct gatgttttcc
     5341 cgccctgacc ccgagagcgg caaaatgata tacgcgccca tggagctggt ccgtttcgcc
     5401 atccccaggc gggtatacac ccgcagccat ctggattacg tgggggaggc cgccgcggcg
     5461 ctggtgcgca gggcggggaa cttgcgcggg ctgcgcataa gctgggcgcc tccggtgctg
     5521 cggcacttca ccgcccatct ggaagaaacg ctggaatgaa atcctaacct gaactggaaa
     5581 gaacaagcgt tctattatgc cgggctgtgg gttggttggt tcagttactc ccgggaatgc
     5641 tcgaacgggg tggggggtac ggctcgtggg ctctcccggg gctgaaccaa tccgcggtct
     5701 tggagtaaaa tcacatcgac aatcgtgaaa tcagcgattg atcagcgtaa tgcgtaaagg
     5761 agctttggaa tgcgtgttct catcattggc accgggtatg tgggtttaaa tacgggggta
     5821 gtcctcagct atattggtca ttccgtgacc tgcctggata ccaacgctga aaaaattaat
     5881 ctgctgcgca gcgggaagac cccgttttac gaaccgcatc tggatgagat gctggcggaa
     5941 gtcggtccga aaatgcgctt cacgaccagc tacgcggaag ctgacctttc gaccatggat
     6001 gttgtgttca tcgcggtggg cacgccctct cttcccgacg gcaacgctga cctgagctat
     6061 gttcgccagg ctgtggagga aatcgcgcag gagatggacg accacttttt ggtggtggtg
     6121 aacaagtcca ctgtcccgat tggttcgggg aactgggtgg gttcgctcat taaggagagc
     6181 tacgagaagc gggtcggcgc gaaagcggac ggacgcttca gcgtggtttc caatcctgaa
     6241 tttctgcgcc agggcgcggc gctctatgat tccttctacc cggaccgcgt ggtggtgggg
     6301 tccgagaact cccacggcat ccaacgcctg agcgaactct tcgcgcccat cctggaacaa
     6361 aactttcgcg cgccgacctg cctgcccagg cctgaagggc tgaaacaggt gccgctgctg
     6421 gtctgcgacc tgaccagcgc tgaagtgatt aagtacgccg ccaatgcttt cctggcgctt
     6481 aaaatttcgt acatcaatga gattgcccga ctggcgcagc gggtgggggc ggatgccacc
     6541 ctgattgcgc gcggaatcgg tctcgacagc cgcatcgggc cgcgcttttt gaaccccggc
     6601 attggctggg gcggttcctg ctttggcaag gacacggctg cactggtggc aaccgcgcgc
     6661 gactaccagt tggagatgcc cattgttcaa gctgcccggg atgtgaacta ccgccaacgc
     6721 gcctgggtgg tggacgcgct gcaggaaggc ctgaagattc tgaagggcag gcatgtcacg
     6781 ctgctgggat tcagcttcaa accggatacc gacgacctgc gcgacgcgcc ttcgctggat
     6841 atcgcgcgac tgctggtgca gcgcggcgcc attgtgcgcg cgcatgaccc ggtggcgctg
     6901 aagaacgccg agcgcttgta cccagagctt ggggtaagat actgccagga cgtgtatgaa
     6961 gccttgcatg gggctgaggc aatcgtgctg gtgaccgaat ggccgttgta ccgcgagctg
     7021 gattgggaga aaattccgcc cgtgctggtg gtggacggac gcaacttttt ggatcgcgaa
     7081 aagctgaaga acctcggctt tcgcgtgctt ggagttggcc gctaggtaaa gaaattcgcc
     7141 cggcgagtcc acgcgcgctt gcgcatagcc ataatttaca ccccacaacc tgaatccata
     7201 gtttgagcca cccacgtgga aagcgtgctt aacgcgccgc gtggaacgga gtaggcgccc
     7261 tggatttcac aatctgcgta taatggtgtc tgcttaaaat aatacaatct caggaggaat
     7321 tcatgccaga aatgttttgc taccaatgcc aggaaacatt acaaaatacc ggctgcgtcc
     7381 ggcggggtgg ggtgtgcggc aaatcagcgg aggtctcgaa cctgcaggat ttactaatct
     7441 acctgctgaa ggggatttcc ttctggggca cgcgcggacg cgacatgggc gtagtcaacg
     7501 aagaggctga cttgttcgtc gctcaggcgc tgttcgcgac cattaccaac gcgaactttg
     7561 acccacagcg ctttgtggag ttaatcaaaa aagccattct cttgcgcgat gacctgcgcc
     7621 aacgcgcgca ggcggcttgt tacgagcagc acggcggcga cccatgccgc agcagcggac
     7681 ccgattgggc tggctggcat ccggcagatt acacgctgga aaccctgctg gaaaaagccc
     7741 gcaccgttgg catcctggct gacgcggagc tcgatcctga tgtgcgttcc ctgcgggaac
     7801 tcatcgtcta cgggcttaag ggcatggctg cttacgcgga ccacgcctat gttgccggcg
     7861 gtcgggatgg ttcggtgctg gcatttctac aggaagccct ggaagcgacc acccgtaagg
     7921 atataggcgt gaatgagctg ctggctgtgg tacagcgcac gggagagttt ggcttgaagg
     7981 ccatggcgct cctggacagc gccaacacca accgctacgg tcacccggaa cccacgctgg
     8041 tcaaccttgg cgtgcgcccg gggcctggca ttcttgtgag cgggcacgac ctggtggacc
     8101 tggatgagct cctgcagcag accgagggaa ccggcattaa tgtgtacacg cacggcgaga
     8161 tgctgcccac caacgcctat ccgggcttga agaaatatgc tcatttggct ggcaattacg
     8221 gcggctcctg gtggaagcag tccagggagt tcgaaagctt taacggcgcc atcctgatga
     8281 ccaccaactg catcaccccg gtgcgtgact cgtaccgcga ccgcattttc accacgggca
     8341 tggctggctt ttccggcgtt ccacacatcg cggaccgcga accaggccgg cagaaagatt
     8401 tctctgccct gattgcccgt gcgagaaatt gcgaaagccc gctggaactg gagaacggaa
     8461 ccatcccgat tggctttgct catcacacag tgctaagtgt ggcagacaaa gtcatcgaag
     8521 ccgtcaaaag cggcgccatc cggcgctttg tcgtgatggg cggctgcgac gggcgccaga
     8581 aggagcgcga ctacttttca caggtcgcac agaaattacc gaaggatacc atcatcctga
     8641 ctgccggctg cgcgaagtac cgctacaata agctggggct gggcgagatt gcgggcatcc
     8701 cgcgcgtatt ggacgcgggg cagtgcaatg actcgtactc tttgattgtg atcgcgcagg
     8761 cgctggcaca ggcgtttaag gtttcggtca atgagctgcc gctttccttt gatatcgcct
     8821 ggtacgaaca gaaggctgta atcgtgctgc tggcgctgct ggccttgggt gtccgcaaga
     8881 tccggcttgg accgacgctg ccggctttcc tctcccagaa tgtggtcgat gtgctggtga
     8941 aagcctatga gctccaaccg attgcagacg cgcaagagga tgtccaggca atactggctg
     9001 gtcattagta aagggattga gcgaagcttg aaggaggcgc ataacgaatg gtatgcgcct
     9061 ctttctttcc atcaatcgaa gcaagcagga tttcgcagtc cataagcgcc ggacaagagg
     9121 ggccaaatca caggcacgcg ctggtttccg cgccaacatg tcgtgagcgt gttttccggc
     9181 gatttgtggc aaattcgcgg gtctgaggca gcagcaaaag gcgggctttc aaactgaaag
     9241 tcgctgaacc cggttctatc aaacccggac ggcgcaataa cctgcttcac gctgccggcg
     9301 cttggcatgt tcctgcggcg cagagtttct tccttggaca ggcttttttc gtaatcaggc
     9361 cggcagcatg gcagtcttca ttttccagcc ggattttccg cagggtgagg tgcagcgcgg
     9421 cgaaaagaaa agaaaaatta ttacttaaga gaataacact tctcgatagt aataacatag
     9481 aaaacaacgc agtcgcgcca gtcagagcgc ctttatcagg tttcctgctc ctgcagccaa
     9541 gacaggcgct tcaacgcagt gaggattacg acgcgcgata gtgcagcgcg tttttaggct
     9601 gccaatcttg agcgctcttc cagcaccagg cgcaggtgcg gccgccagat gggaaagtat
     9661 tcctgtattg tttgcggatt tgactcgaaa gtgccgccga aagaaagctg cggtctggcc
     9721 tgcccaggcg cgcagtctta gcactttgag acataactct aaagcaagcg ggccgacagg
     9781 tgaggaacgc aagcttgcgg gcagccccaa ccagaaggcg ttccaggcgg ccttccgccc
     9841 tctttttgcg ataaaataag ctacgcgcag ggctggagcc gctgtagagc ccgctgttaa
     9901 aaacaggcgc agtgcgagca ccaattgcgg cccgaacgcc cctccagact cgcgcgagta
     9961 tctcaatttt tctttttccc aggcccgaat gctgtgccca cgcgcacaag gcagcctccg
    10021 ggctccccca ccccgcgctt tccaggcgct ggtacaatta cttatgagaa tgcagtccaa
    10081 aaggagcgct tatggcaggc aaatccaaat ccagatcccc gctgaacctc cccgacctga
    10141 aaacccttaa gccgcgcgac cccgctgctc cctcccccgc gcaggcgtac ctgaacgcgc
    10201 tcagtccatc gggcaggcgc tcgcagcata cagcgctgaa caacctcgcc tacatccttt
    10261 ctggcggggc gctgaacgat gcactgactt tcccctggga gcttttgcgc tacgagcata
    10321 ccagcggcct ggcagcgtac atggtggata tcggttacgc caggtcttcc gtgaataagc
    10381 acctggtcgc gctgcggcgc gttttggcag aaagttatca gcttgggctc tatgccgatc
    10441 acaacgatta ctaccgcgcc gccggggtta aatctttgcg ctcggaaacg ctgccgcgcg
    10501 gacgcaccct cagaatggaa gaactgcgcg cgctgattca gacctgcctg aaagacgcga
    10561 aaaacccgac tctgggcgtg cgcgatgcgg cggtgattac ggtcctctac gccgcggcgg
    10621 tgcgccggca ggaaattgtg gacctgaacc tggctgacct tgacctcaag gagggcaagc
    10681 tgcgtatccc tgggaaaggc agcaaaaaac gccaggtgta cctgcaggcg gatgcggtca
    10741 gagcgctgca gaactggttg gcgctgcgcg gcaggcagct cgggccgctt ttcacgcgcg
    10801 tgacaaaatc cggccggatt ctgctgaaac gcctgacgcc ccaggcggtg tattacctgc
    10861 tgaagcaccg ccaggctcag gccggtttgg acgacttcac cccgcacgac ctgcgccgta
    10921 cctccatcac cgacctgctc agcgcccagg tagacgtgct tacggtctcc gcgattgccg
    10981 ggcacgccag cgcggatacc accaggcgct acgaccgccg cagcgatgaa aacaaaaaag
    11041 ccgccgcgca gaagctgcct tcgccctttg ctgacccccc ctcccctacg gaaaacccgc
    11101 agcaggaaaa gggcgtgcat ccctcagacg ggaatccgca ggaataaaaa aaaccgccgt
    11161 ggagactcca cggcggctgc gcagcttgag gaggaagttt aacggctgac cagccagccg
    11221 ataatgagct gcttttcttg cgcgttaatc tttgcgccgt acccgatcat gcggtcaagg
    11281 attttggacc attcgtccgc cgtacgcttc tgaccgagaa taaagggcag gacgtgcggg
    11341 ctgccctgca gcttttgctg aatcaaagct tccatttccg cgtccgagag tgtggatggg
    11401 gtttgagcgg cttccccgga cgcgggttgg ctggtttcca cggcaggtcg ggttcctttc
    11461 ggagcaccct tgggcgttcc ggcgctgctg gcaaaggctg tagggatcat aagcaccaga
    11521 gaccctgcaa taacgcttaa tatgtttcgt ttaaacatag caacctcgat tgttatctgt
    11581 ataccggaat acccggcgta ttgttactat tattgtatta attgacacgt tgaaattcaa
    11641 gcctcgcggc tggaatccct atacaaacca tccaactgag ggggggttag taaagcaggg
    11701 aaagcagctc cgccagagaa aagcccgcga agtattggtt gacctcgaag gctgtcagac
    11761 gctcccccag cgcatctggc aggtatgggc agccagccag gcgctcctcc aattcagcca
    11821 cgtcgcgctg gccgaaaaag tcgccaatga atttaatcga gtgcagcatg cctttctgga
    11881 cgaccaggta cacttcaatt tcaccgccgg catagcgcac cgagcgccgc aaattgaatg
    11941 cgggcgagga accatagttc caatcccagg actggtagcg ttcgcgagcg agctgccgca
    12001 cagcttccca atccgccggc gtaagttgat actccgggat atctgcggca gtttgctgcg
    12061 caaaaacccc cgacagaatt tgccggcgga agctgaggat gtccaccggc tcgctcagga
    12121 actcagtaat gttggcaaca cggctgcgca cggacttgat tcccttggac tcaattttgg
    12181 aagcttttac gcgcagcgcc ttgccaatgg cctccaggtc cgagttatac agcagtgtgc
    12241 cgtggctgac catgcgcgcg ccggaaacat actgcgcgtt tccggagatt ttgcgccctt
    12301 ccgccaggat atcgttgcgc ccgctcagtt cagcctgcac ccccaaatcc cgcaagacct
    12361 ggatgaccgg cgcggtgaac ttctggaaat ttgcaaagct cccgcccagg ttaggcgcga
    12421 taaagctgaa attcagatta cccaggtcat gatagaccgc gccgccgccc gaaagccgcc
    12481 gtaccacatc cagcccctgc tcccggacgt actccacgtt gatttccgcg aatgtgtttt
    12541 ggtgcctgcc gatgatgatg gaaggttcgt tgatatagaa cagcaggagt tcctctagcg
    12601 gggcgatgtt ccggatcaga tattcttcca gggcaaggtt cagcgccgga tcgtgcagat
    12661 tttggttatc gacgaaaagc atagtcacct tctgccaaac atggacatat gctggattgg
    12721 gaatgcgtga aattttgcaa tccaggaaat tgattatatc cgaaagctgg tccggaaaat
    12781 tcggctgttc cgtcgtggga agagcccccc aaaccgaaag gcaattgttc cgcttttcac
    12841 attctgtggt atatttcatc cgtttatgcg gatgaataaa cggatatgag cctcgaaaac
    12901 gaaactatct ctagtattcc ccacggcagc gtcacggaag ctgaaattct gctgcttcac
    12961 cagcaagtgt gctacgccct gggcgatcca acgcgcatta tgctgctcta cctcctgcgg
    13021 gaaggtccgc aatccgtcgg ggaactgacc gaacggatga agctcccaca gtccaccgtt
    13081 tccagacact tacgggtgct gagagagcgc tctctggttc tcgtggaaaa gcgcggcact
    13141 tccaacctgt acacaatcac cgacacgcgc attaccgacg cgctggattt attgcgtcag
    13201 gtgcttaatc aacagctgtc agaacaggcg aaattgacgg ctgcgattca atgttcttat
    13261 ccgccaaacc aagatttcag cgacaaggag taaggtatga aagttgtaat tgtaggaggc
    13321 gtcgcgggcg gcgccagtac agcagcccgt ttgcgccggg ttgatgaatc cgccgaaatt
    13381 atcatgttag agaaggggcc gtatgtctca tttgccaact gcggcttgcc ctaccatatc
    13441 ggcggggtga ttcaggaccg cgaccagctg ctggtggtca cgcccgaaac cctgcgggat
    13501 atgctgaaca tcgaggcgcg gaccaacagc gaggtgcgct caattgaccg gcagaataaa
    13561 accgttgtgg taaaagagct gaccaccggc cgggaatatc aggagcctta cgacaagctg
    13621 gtgctcgcca ccggcgcggt gccgattgtg ccgcctctgc ccggggtgga cttgccagga
    13681 gtctacgttc tgcataacat ccctgagatg gatgcgattg aaagctgcct gagcgaagcc
    13741 tgccccaaga gcgtaatcgt ggtcgggggc ggtttcatcg gtctcgagat tgccgaaaac
    13801 ctggcagaaa aaggcctgca ggtcacggtg gtggaaatgc tcgaacaggt tatggcgccc
    13861 gtggattttg aactggctgc catggtccat catcatctgg cattccacaa catcggtttg
    13921 tttttaggcg acggcctgaa gagcattgag aagacaggcc cggacggcag cctgcaggtc
    13981 aatctggcca gcggtaaatc gctgccgacc gatatggttg tgctggcgat tggcgtgcgc
    14041 cctgagactt ccctggcaaa agccgcaggc cttgagttgg gcgtgcgcgg gacaatcgcc
    14101 acaaacgacc agatgcagac ctccgacccg gatatctacg ccgtcggcga tgacgcccag
    14161 ttgacccaca acatcctcga gcagccgaca tttttggcgc tcgcaggccc tgccagccgc
    14221 gaaggacgca ccgccgcgga tgcaatcgcc ggcagaagca caaagtttcc cggcgtgcag
    14281 ggcacttccg tcgtgaaagt gtttgacctg acagtggctt cgacgggttt gaaccagcgc
    14341 caactggagc aggctgcctt gccgtttgaa tcggtgatta tccatgccaa cagccacgcg
    14401 tcctactatc ccggcgcggc ccagatggca ctgaagctgc tcttctcccc gcaggacggc
    14461 cgcatctacg gcgctcaggc agttggaccg gacggcgtgg acaaacgcat cgatgtcatc
    14521 gcgaccgcaa tcaaaggaaa cttgagcgtg tacgacctgg aagaactgga actttgctac
    14581 gccccgcctt ttggctcctc gcgcgacccg gtgaatatcg ccggttttgc cgccggcaat
    14641 atcctgcgcg gagacgcgcc ggtgctgcat tggaatgagg tggatgggct ggaccgcagc
    14701 acctggttca tcctggatgc ccgtctgccg gaagaaggca cgctcggcag catcgcgggc
    14761 agtcgcaaca tccccatgca gcagctgcgc cagcgtttgg gcgagcttcc ccgcgaccgc
    14821 aagattctgg tgtattgcgc ctcgggacag cgttcgtact ttgcggtgcg gatgctgcgc
    14881 cagaacggct ttgatgccta taacctcagc ggcggctata aaacctacgc ctacgccgtc
    14941 agcccacaat cccatacccc tgaaccggag accaagacga tgacccttcc cagcacagac
    15001 gccattcccc aagcccccgc tggcagtgaa taccagctgg atgcctgcgg tttgcaatgc
    15061 cccggaccaa tcctgaagct gtacaacaaa gtcaaggaaa tggaagacgg cgatgttgtc
    15121 acggtaaaag ccaccgatta cggctttgcc aacgacgtcg gctcctggac gaaggcaacc
    15181 ggcaacaccc tgctcaacct tgatagtgcc ggcggcgtga ttaccgcccg cgtacagaaa
    15241 ggacagcatg ccgaagaagc tgccgcggct tccgggacgg gtaagaacgg caaggacatg
    15301 accatgattg ttttctccgg tgacctggac aaagctattg ccgcctttat cattgcgaac
    15361 ggcttcgctt cgatgggcca gcgggcgacc ctgttcttca ccttctgggg cttgaacctg
    15421 ctgcgcaagg acaatccgcc cgcggtcaag aagaacctgg ttgagcgcat gtttggctgg
    15481 atgatgccgc gcggcgcgaa aaaagccgtg ctgtctcaga tgcacatgtt gggtttgggg
    15541 actggcatga ttaaggggat catgaaacaa aagaacatcg attccctgcc tgtgctcatt
    15601 aagaccgccc aacaaaacgg cgtaagactt ctggcgtgcc agatgaccat ggatttgatg
    15661 ggcatcaagc gggaagaatt aatcgacggc gtagaagttg ccggggttgc caccatggct
    15721 gccgatgcga gcaccactaa tacgcatttc ttcatctaga agagaagcag gttttcaatc
    15781 gaaaggcgga tgcgagattg catccgcctt tttcgctttg atatcctaaa ggctgccgtc
    15841 cgaaactaaa gaatccgtaa gatggtcgat agagcgccag cgaaacgtat cgagaatgca
    15901 gcctggctgt ttggacgaac tggttcgcga aaacgtgcta ataggtttcg gtaatcttag
    15961 acaggccgcg gggatggtcc gggtccaaac cgcgcgagag tgccatctgc caggccaaca
    16021 cctgcccggg gatgactgcc gggatggggg aaagccattc cggcgtgcct gcagccagcg
    16081 gcagagccag ctgcgccatc ccaagcgctt cttcccggtc tgaaatcacc accaactcgg
    16141 cgccgagccg gcgggctttc cgaatcaacg cggcggtatc gcggtacatc acgcctgaag
    16201 gggcgacggc cagcagcggg tagcctttgt gcagcgaggc cactggtccg tgcaagaagt
    16261 ccgcggagga atacggcacc gtcgtaacgc cggtgagctc cttgattttc agtgcaatct
    16321 cgaacgcggt gctgtagtta taaccgcgcc cgatgacggc gccgtgctgc atgaagcgga
    16381 aacgctccat gcgcggcgta agctccaggg agagcgccag cgccgcttcc gcctggtctg
    16441 gcagcctggc gagctcatcc agccttcgtt tctcaccgga aagcgcggcg gaaatcaggg
    16501 cgaccgcggc cagcgagctt gtgtaggttt ttgtcgctgc caccgaaagt tcgggtcccg
    16561 cgccaagcgc aagtgtgcaa tccgcagaag cttcgagcgg cgagccgggg atgttggtga
    16621 tggcaatcgt cggccgcttt tgtgccctgg cggcatccag aacggatatg acgtccggag
    16681 acttgccgga ttgggaaatt cccagtacca gcgcgccctc aagcagcggg ggcttgcgat
    16741 acacggaaaa aacagacggc gttgccagcg cgaccggaat gtggttgaca atttgaaaca
    16801 ggtattgggc atagcgcgcc gcgttatcag aactgccgcg ggctgcgatg aggatgtagt
    16861 gaaaacggcc tcgcagcggg ttaatcaatt cccgcgcctc gtccacgccc cgcccgagta
    16921 agcggcgcag aaccgaggcc tgttccagga tttctctctc aagaatagaa gcagtcatct
    16981 gcaatcccgg aaatttttag ctgttaaacc ccgacgtttt gtagcgccag cagtctttca
    17041 ggtactcgtc gtccaaaagg taaaagacgt cgcaggcttg ctgcaattcc ctggaagaga
    17101 tggttctggt ggtggcgttt tcaacgtgct tgcctccccg cttgacaacc tggactgccc
    17161 ggcaaaaatc cgagtcaccg gagatgagta ccgcggtttc atatgtgctt tgcaggtcga
    17221 gcatatccac ggcgatcgcc acatccaccc ctttttcgat gtaagaagaa ttgtgcaaaa
    17281 ccaaccgccc caggacaact tcaaaatatg gggtgcggcg cagcgcctcc aggaacttca
    17341 tctggcgttt gtagcgttcg atttccgtca ccaggttggc ttgctcctcg gtcggagcga
    17401 cggcgtaata gtagactctg gataaggtcc tcccgttcag gagtttctga accagtttct
    17461 gaaagtcgag gtcaagcctt ttgagatctt tgattagccc gaaatataga tttgttccgt
    17521 caataaagaa agtaactctt tcactcatgg ccctcccatg cttttttcag gattgcgttt
    17581 caatcccgga ggttttgttg cccgaaatca ttgagagagt ttcaatttca ggatgtttgt
    17641 tggtcaatag cagtgtttta tacaggatat ggtcgtgata atcaggtttt cttttcacag
    17701 ggcaagctcc cccgctgcac agcgcgggtc tctctcacag caactgccgt tccgggaatt
    17761 atagcataga aaggcagcag cggatttttc cggatgtgtt ttgatgatgg cataatatgg
    17821 ctacgcgcag cttcctgtcg cttaagcgac agcctgcgga ttgctgacgc ctggcaggcc
    17881 ggcgaaaatc gggttctcag caagcgtatt caggcggatg aggtaacgct ttcaatggaa
    17941 aattaaagcc agtattgtaa ctccgcgcat gcccaaaacc gcatcagcag cgcccttctt
    18001 ctcgcattat gcccctaagc acaaatcaga cagaagtttg ctcgcttttc aggcggcttg
    18061 cgcggcgcgg aagatctttc acccggctga taatcgccgc tccgagcaag gtcagcgcca
    18121 ggcagacggt gaacatcacg ttatatccga aagcttccac cagagcgccg gcaaaaacag
    18181 gcgcgacgag cacaaaaatt cccgtcagac tgcgtgccat ccccagataa gtcgggcgca
    18241 gcttctcctc gcccagttcc atcaccagga tcgaatcccc aagcaccata ccggcagagt
    18301 ttaacccgaa catcaaaaag accaggtaat atgcccattc gctgcgcgcc aggatggcaa
    18361 tcagcatccc cacagcccag atgaggacgg acgccaccac gatgcgcttc ggtcccactt
    18421 tgtcgccaag catgccgccc agagtgtagc caatgattcc gctgaagtag agcagggcag
    18481 taaaaacagc cgcgcgggaa tcagacaggt tgaagcgttg gatcccgtag actgccagaa
    18541 atccgcttgc catcgcgccc agaaatatca ccgaacgccc gaccaggtag tagcgaaagt
    18601 tcttgtcgcg cttgagaatg gcgccaaaga gttccaggtc gatgattttc cgggctggtt
    18661 tcgttttttc ctgttccatc cgtgcgcctg cgctggaggc cgggtcggcg cttgccgttt
    18721 ccagctccgt gtttaccggt tcgcgtatac gcgagtagaa atagaaagaa aaccactgcg
    18781 ccaccaccgc ggtggcaaaa cacaaggcgt agttttgcgg gtacggcaga cggctgagga
    18841 aaatggtggc gagcacagag ctgagaatgc ccataaactg agccagcacg cgcgataccc
    18901 catagaaacg ggcccggtgt gtgatgggta tgacgctggc ctgcacttcc tgccagggca
    18961 gcgcgattaa gccgctggcc agcccacgcc aggtaatcag gatgaagagc cagaggagcg
    19021 ccgtggtttt cggaatgctc ggcaccaaca gcgccagcag ggggaaaaaa acatacggaa
    19081 cgcgttccac cacggccatt ttgaccgcga aaggatgctt ctgtttgagc cccgaaacat
    19141 agttggagag aaacatttgc ggcatatacc agccaaagtt cgcaatggcc gggattaacc
    19201 cgatcagcag cggtgatttg gtcaacgtcg cggcgaatac cggcatgatg gtggtaatgg
    19261 aaccaaagcc gtccccaaag ataaaaaaca tcaggtccag gcttcccatc gtcacgttgt
    19321 gcctgaaatt ctttgccacg ttgtgcggaa cttcatcctc cgggcggata gtaagaagac
    19381 ctgtgagaaa cgattttaga cggttagcca tatgcgctgc aattcgtgga atcttcaagg
    19441 cacaattata gctgggaaac gctgagatga cagatttcac tgatcttgca gcgcgcctca
    19501 gcaaaatcag cccgctaccc gcccgttttg ttcgcgagga ttagcacact ttcggcaggg
    19561 atatggacgc gcccgaagac tgcaggcaga tgttcgcctg tcagcatgtc gaaaacggac
    19621 tggtcacctc ccaggcaatc ctgagggaca ggaatggtgc ggggctcctc cgagcggttc
    19681 agcgcggcga agaaggacgt atccgccagg cgcacgcgcc acagggcagt ttccctctcg
    19741 tcatcgagcg caagcagaag ccagtccgca tccaccaggc ccgggaaacg ttcccgaaag
    19801 gcgctcaagg cgcgaatgtg gtccgccaac tcgctgtgct ggtcgacttc accccaattc
    19861 attgccagac gggactggtc aaagcccata gcagccgggt cgtgtataga gagcgcctgg
    19921 gaaagcgcgc tctcgctgcc gaaatatacc agcggctgcc cgggaagaag gtagagccag
    19981 gagagggcga gcttcaggcg cctctggctg ccgccggcgg caaacaagaa gcggttcatg
    20041 tcgtgattat ccaggaaagc cggacggctg aattcccgtg gaaaataagc ctggctggcc
    20101 tgcaaaaacc cggcaaaccc tgagataggt ttagagcgga aagcgaagct ctcccgcagc
    20161 gcctggcagc tgaggaagtc cagcgtgccg tccatccctc cggcaaagct gagctgctgg
    20221 tcggccgggc gcgttacctc gccaaaggtc cagacttccg ggttcacctc gcggcagatc
    20281 ttgcgaaatt cgacccaaaa atccaggccg ggaccaatgg cgtaatccag acgatagccg
    20341 tccgcaccca ggcgcagcca atagcgcgct gcatcaaaga ggtgctcacg ggctggcgac
    20401 ccgtgcgcca ggttcagttc gggcatgctg cgcaggttaa aataaccctg gtattcaggc
    20461 cattttaccc acttaaacca ggcggtaaag gggctggagg ccgatttaag cgcttccagc
    20521 agcgccgggt gttggtttga aacatgattc gccacaaaat ccaggatgac ccgcagtccc
    20581 agcgcgtgcg ctctggtgat cagctcttcg aagtccgcaa gcgtgccgaa gcgaggttcg
    20641 atgcgcaggt aatcgctggt atcgtacccg tgatggctgg ggctggcgaa gatcggtgtg
    20701 agccaaatcg cgttgaatcc caggtcgtgg atgtaattca gtttttcgat tactccgcgc
    20761 agcgtaccgc cgaacggacg cgccaggtct tcctgctgaa gccaatccct cccctgcccc
    20821 gggttgaaac gatcgacaaa aatctgataa atgcgggcag atctgctcca ggcaggaacg
    20881 ctgtccgcgc cgtaccaaat cgcgaactga tccgcgctgc gcgcgtcggc ggactgagtg
    20941 tcggcaaaga gcagccctgg gctggtctgc ccggcttccc gttctggcag gcgcgcgtaa
    21001 acctggtagc gcaggagcgt ccccgcgggc tgcggcggca gcagagcgcg ccaaccggtt
    21061 atccaggccc acgcgagcgt gtcccagttt tgcccttcgg gttggaaggg cagctccgcg
    21121 taatcgcgcc aatcgttggt ggagtagcgc agcacaagat ccccgacggg acaaccgctg
    21181 gaggtatacg cgtaaagcgt gaccgcgtcc ccgggctttg gcacgagcgg ggatttccgc
    21241 ccgaaatggt gcacgcccag cgcgcgatcc attaaaaacg ccgccccaat ggagggcgtg
    21301 tccaggtccc caaagctgtg gtcgcgtaaa gattccggaa tttcagtggt catcattctc
    21361 accgctctgt ttggatttga gcaaaagcaa tcgtgttcct cggggagcgc agttcgcgcc
    21421 ttgtcgtgcc gcaattgtaa aacactttgg gcaaaagcac ccgggcgcgg ctctgctgca
    21481 aagcgcttgg taaagaaaaa ggcagcgcat ttccgcgctg cccaattcgg ttgatagtag
    21541 attacgcctc gggcggtttc tgaaagtttt ccgcttctgc ggcgtccgcc ttcgaggcaa
    21601 acagcttcag gaaacgcttc cagccttcat gcaaattcgc cttttgaaag cgccaggtgc
    21661 caacgatgcc gtagggcagg aacatcacga tgaggatgaa cagcgcgcca aaaatgagcg
    21721 gccagcgcgc gccaaaccag gtgtaaaaga actgctggat aatttggctg attgccgcgc
    21781 cgaagatcgg accgatcagc gtccccatgc cgcccatgat ggtcataatc aaggcgttca
    21841 cggttgtgac caccgatatc atcgtcggag tggcgctcat attccagatg gagtagaaca
    21901 tgccagccaa acccgcgaag actgaggcta ccacaaacgc aatcgttcgg taagtgacag
    21961 ggttgtagcc aatcatgcgc atgcggtctt cgttttcccg gttagcgaca ataatccgcc
    22021 cggtaggtga attgacgaaa cgccgcagga gcagatagga aacgaacagg aaggcgagcg
    22081 tgataaagta gacggtcaag cggttttggg tcgggttcag ccaaaccggc agggggatgc
    22141 cgtgcagccc ttcgtccgcg ccagtccatt tgtgcaggtc ggttgatttg atgatgatat
    22201 aaaaagcggt ggagagcgcc agcgtaatca ttgcgaagta cgtgcccttc aggcgcatcg
    22261 aggtaaccgc aaagagcagc ccgatcgcga cggaaacaag cagcactacc agcaagacaa
    22321 ccagaagcac gaccaccatg gtcaggtcgg tattgccaag gacgataggg ttgtttttaa
    22381 tgaaatttgg cgcccaatgc ttcatcagca ggccaaatgc gtaggctccg ccgccgaatg
    22441 aagcggcatg accaaaggaa aggattccgc caaagccaat caacaagtcg tagctcatgg
    22501 cgaagactgc cataataaag aaagcaataa gctggccttg ccagaatttt gtcacgcctt
    22561 cattcaggcc ggtgccggta atcagattca tcacaaaggg gagtaagacc atcagtccaa
    22621 tgaagaccag gaatccgagc tccttaatct tgaatggagt ttttgcagcc tttttggtga
    22681 caggattttc agccatgaca ggttactcct tctttccaaa caagccttgc ggctttgtga
    22741 gcagcacaat caccataatg atgaccgtcg aagcttcagc aatcgcaggc gaaagggaga
    22801 gtttgagggc aaagtaatcc ccaaaagcgc gggccatgcc caggatcatt gctccaacaa
    22861 acgcgccaac atagctgccc atgccgccaa tcaccacggt aatcaagccc tgggtctggt
    22921 aagtatcgcc catcagcggc tgcaccggca ggaagggcgc cgcgccaatg ccgccgagcg
    22981 ccgccaaggc cacgcccatc gcgaagacca gcgtaaagac ggctttgacg ttgatgccca
    23041 tcgcctcgac catttgcggg tcctgcacgc cagcccggat agtcatgccc aggcgggtcc
    23101 tggtcataac gaaaaggact acaacaaaca tgacaattcc cagagcgatg atgaacaggc
    23161 ggtaggttgg aacagtcgcc ccatagatat cgatggtcgc gctgcccttt tgcagccatt
    23221 cggcaatact cgtggctttg cccggttggg caaacagagg cgggcgggac atctggtagg
    23281 tcagcggatc ccacaggtac tgcaccagct cgcggatgac atagctaagg cccagcgtca
    23341 tgagcaggat aaaggtcggg cgcacgtaca gcgggcggat tagcgtgatc tcaattacgg
    23401 cgccaaacac gaacccgaaa gcaacggcaa gcaggataga gagcacgaag cgcaggttgc
    23461 cgttcatcag cagcaaagcc tccgacgcgg attcgcgtac cagcgtgctg aggaccgtga
    23521 ggaccaggag cgccgccggc atcaggagtt tcttcccgat gggttccctg ggggcaaact
    23581 gggttttatc ccccggcctt gaggaagcca ccgcgattaa caagccggcc acgaacatca
    23641 gaagcgggcg cagccagtag gagctaagtg gctcctgagc gctaatttct gccagtggat
    23701 tcccaattgt ggtggtagtg gcggccacca tcgcggtatc ggccacgctc agaatatcga
    23761 aacccacgaa ggcgaacaca agcgcaagca gcgccagcac ataggcgatt tttggaagcg
    23821 ccttctgcat tttttcagaa actttccagg cgatgaggta tggtttaata ataggcgtga
    23881 gcattacgcc agcgccaaat gccagcagga aaggcgccat accaaaaata aaggtggggt
    23941 tggtgtagaa ctgccagcca atatacgctc cgagcataaa catggagccg tgcgcaaagt
    24001 tgagcacgtc catcaggccg aagatcaggg taagacctgc ggcaaccatg aagaacagcg
    24061 agcccagcgt tagaccggag aaaagggtgc tgataaatgc gcgttgcccg gcatatgtgt
    24121 agacgcccac caccaccgcg accaacagcc cggcaataag aagtccgagg aggatatttt
    24181 tacttttcgg attcatgttc catctccagt tctttccggg tcttgatact cgcgccaaga
    24241 taatggtgga taaggtctgt gtcctctacc agatcctcca tcaaaccttc ctgaacagac
    24301 tttccatcgt caataatggt ataacgctcc gccagctgac ttgccatgat aaagttttgc
    24361 tcaaccagca aaatggtggt ggtctggctc agttggcgga tagcttccat catctgttga
    24421 atcagaatag gagccagccc ttcgcttggt tcatcaatca ggagcagctt attgtccgga
    24481 accaacgcgc gcgctaccgc cagcatttgc tgctgaccgc ccgagaggtg gttaccgggc
    24541 agcttgtaaa gccgcttcaa atcgggaaac agttcaaaaa tgaagtcgga cttgcgctca
    24601 aaatctcctt tttttcgttc cgccagtttc aggttttcca gaacagaaag cgcgcgaaag
    24661 atggcgcggt tttcgggaac gtagcccatg cccaggttgg caatgttgaa ggcttgttta
    24721 ccctggattt ccagcccatc gaaggtaatc ttcccttcgc ggggcggcgt cagcccgatg
    24781 atcgacttta aggttgtagt tttgcctgcg ccattgcgtc ctaacagcac cgtgatggaa
    24841 cctttcggga cgcgcacgga aacattttcc aggatgtgaa attgaccgat gaaggtttca
    24901 atccggtcaa caacgagcag atcattcgcg ggtatttgtg cgctcatgct aattctccat
    24961 acagatcccc caaatacgcg gtctggacct gcagattgct ggcaatctca tgcggactgc
    25021 cctcggcgag cacccgcccc tggttcatca cggtaatcac atcggagata gacatcacca
    25081 tatccatgcg gtgctctacc aggatggctg tgcggtcgcc gtgattaacc acccgcttga
    25141 taatgtttat caggtccggc acctgttcgg aagacattcc tgcggtgggc tcgtcaaaca
    25201 gaagcagttc cgggctgcag gcaatcataa tgcccagttc cagcttgcgc ttttcgccgt
    25261 gaggcaggtt gcgcgcgagc acccattccc gtccttccag tccgaccacc ccaatcactt
    25321 cttccgccgc atcgatatac ttccgaaaat tgtccgccag gtgaagcatt ttgaagttat
    25381 ccttccccat cgcttgtgca gccaggcgaa tattttccag gacagtcagg ttcgggaaga
    25441 tgttcgtgat ttggtaggaa cggccaattc ccaggtgtgc ccgtcgttgg ggcgatacct
    25501 gggtgatatc attcccttta aagatgacgt gcccgctcgt gggcggcata acaccactga
    25561 ggaggttaaa caaagttgtc tttccggcac cgttaggtcc gataattgcg tggatggtat
    25621 gctcccgcac ctgcaggctg acattatcca ccgcaattaa cccgccaaag gctttggaaa
    25681 ggtttacagc ttcgattaag acttcattcg ccataagttg tacaccttgt gtccaaattt
    25741 cccttcatta gtttcctgaa tgcagttttc tgtaaactat aaataaaaat aaggccggat
    25801 acttcagccg caccaccagg aacaagcgcg ccgtgtcagg ttcactattc aggaaaatat
    25861 aagtttttgg ttcataggga gggactttcg tccctcccta ttctattaca gagaattact
    25921 gtcccagagt cggcaggtcg ccgcagcggg acgcgtattc gccttcgagc gtgcagggca
    25981 cacccaattc gtcgtacttc gaaacataga tggtttcgaa gaagcggtat tcattgatgc
    26041 cgtcgtcatc caggtcgggg tcaaggttga ccagtttgag gatgttcatc ggctgttcgc
    26101 agacgtgatc ttcggggcgg atatgatagg tgcccttggg gccttcaaag gtcaggccct
    26161 cgagggcggg gatcatcgct tcgccgctgg tgtcgccgcc ggtcttttcg agggcggtcg
    26221 cgagggcgat agcggaagcc ataccgccag cggtgaacag gtcaggatag gcattgatgg
    26281 cgccgtcggg ggcatccttg tacttggcga cgtggtgttc caccagccag ttgttgaccg
    26341 cattgtcagc agcggtgtag tggtaaacgt tcagaccaac ggtacccaat acgtcctcac
    26401 cgaacacggc agtgaaggaa gcgttgtcac cgaagccggt accaacagtc atcacatcca
    26461 aagcgcccat atcagcgaga ccctggaaga gggtcacata accagtgccg gaccaggtca
    26521 ggaagaggtt cttggcgccg gagtccatag cctgctgaat gtagggggtg aagtcagtgg
    26581 tttcgaacgg ggcgtaaatg tccgcgatca gttcgccgcc gaatttttcg acagagtact
    26641 tgagcgcagc gccggaagcc tggccaaaag cattgtcaat accaatgtgg gcaaaggtgg
    26701 gtccgacatt ttccaccagg tacttggcga tgatgagcgt gtcatcaaag ctggtgcgcg
    26761 cggtgcggaa gacatagggg ttgaagtaca caccggtggt gaagtaagaa gcggcgggat
    26821 cgaccatcag gatgatcttg ttttcgagcg caacagtggt cagggcggcc gcagatgcag
    26881 aggacactgg gccctggagc agttcgacgc cgtctttttc gatgagttcg agcgcgaggg
    26941 cgaccgcttt ttcggcgttg ccttcatcgt ctttgacgat gacttcaacc gggcgattgg
    27001 cgataatcca gcgtccctgg tcgtctttct tgccgccgga catatattcc aggccgagct
    27061 cgaaaccgcg ctgctgctgc agaccgtaga gagccatgga accggacatc atgccgattt
    27121 ggccgatttt caggacggga ccgagctctt cagtgggcgc tgcagcgcct tcttcgaggc
    27181 cggtgaattg atcggcggtg tagtaaccgg agtcaa
//
LOCUS       DAOQAD010000015        26755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_1779_length_26755_cov_5.775880, whole genome shotgun sequence.
ACCESSION   DAOQAD010000015 DAOQAD010000000
VERSION     DAOQAD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 26755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26755
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1779_length_26755_cov_5.775880"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..679)
                     /locus_tag="PKL60_02460"
     CDS             complement(53..679)
                     /locus_tag="PKL60_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HNW95031.1"
                     /translation="MKISTLLIDLDDTVYPPTVEVWPLVMKRMFLYMRDFLGIPEDEI
                     PAMRDRLFNRYGTTLRGLQIERGINAKAYLDYVHDVDLSALLSPDPALRSALDRIALP
                     KLIFTNACESHARNVLRLMDLESCFEGIIDVVAVQPYCKPEPEAYHIALELAGSPDPR
                     EVLMVDDRLENVAAASSLGMHTVLVSLLPQDGFRTIPRLALLPDLLGA"
     gene            complement(676..1269)
                     /locus_tag="PKL60_02465"
     CDS             complement(676..1269)
                     /locus_tag="PKL60_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNW95032.1"
                     /translation="MPSKPLQTITVFSGSADHLRQAYLQGAWQLGAALAERGITLVFG
                     GGKTGLMGAAADGALARGGLVVGVINESLNTPRLAHAGLSRMEVLPDLHARKARMSEL
                     ADAYIALPGGLGTFDELFEALTWAQIGLHTKPIGLLNIAGYFDPLLALVDHAIAEHFI
                     YPEHRQLMLCEADPQSLLEKMQAYQAPGNLSRWVERP"
     gene            complement(1347..2306)
                     /locus_tag="PKL60_02470"
     CDS             complement(1347..2306)
                     /locus_tag="PKL60_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNW95033.1"
                     /translation="MEYKDYYKTLGLDKTAKEADIKSAYRRLARKYHPDMNPGKPEAE
                     EKFKEINEAYQVLSDPEKRQKYDQFGSQWQQYRSTGGSPEDFNWGPWRSQPAGGTSYR
                     TVSQEELNQMFGGMGGGFSDFFETLFGGFSGGFNTQSSRQTNPASARRRQSAAPQDVQ
                     HEVEITLEEAFSGTKRLLQFEDGKRIEASIPPGVDTGSRVRLSGQAGGADLFLRIVVL
                     PHAIFTRKGSDLYTRVPVDFYTAVLGGEVSVPTLTGAVRLTIPENTDTGKVFRLKGLG
                     MPSLKNPQTRGNLYATVEIHLPQHFTPAEKDKVRELRNMRRAQ"
     gene            complement(2376..>2760)
                     /locus_tag="PKL60_02475"
     CDS             complement(2376..>2760)
                     /locus_tag="PKL60_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95034.1"
                     /translation="EAVLAQGGVLAIFPEGHVHRLPLGKPLPGASFLALRSGVPILPI
                     GTYSEDEWDLFGALTRKKRRARIITRIGKPFGPLAAGSLDKIPGREEVKAAGWEIMSR
                     IAELLPAHARGIYGSSLQGLELSAN"
     assembly_gap    2761..2770
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2771..3188)
                     /locus_tag="PKL60_02480"
     CDS             complement(<2771..3188)
                     /locus_tag="PKL60_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95035.1"
                     /translation="MSSRKNLHEYKLKYPYPRRRFIRRALKSGIALAASLITNYRIEG
                     QENLPKGGPLLIIGNHFHFLDSIGPIHSTDYLLEFINDAVMPNAPAAMKFLPDLWGTF
                     KILQGTANLEAMRASEAVLAQDEPLLVDRRGEGRQRG"
     gene            3660..4973
                     /locus_tag="PKL60_02485"
     CDS             3660..4973
                     /locus_tag="PKL60_02485"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW95036.1"
                     /translation="MTVENSDSQITQDLPAHWLRNYLPMWVGQAVSLLGSGLVQFALV
                     WYMTEKTGSATVLAMATFVALLPRVLLGPFAGALVDRLNRKAIMIISDSAVALATFAL
                     VVSFHLGITQIWHIYAALFVRSLCGSFQGPALQASVSLMVPREHFARLGGINQAMFGV
                     IDIVSPPLGALLISVMTMQSVLAIDLVTAAIAIALLAFLVHVPQPASVSTTEALTPKR
                     VLADVRNGLKYAVGWSGMFKVIVMASLINMLASPAFSLLPLMVTKVYGKGAADVAWLE
                     SALGLGVVAGGLLLGVWGGFKRKTATTLTGLLGMGVGITILGLTDRSTYMLAVGMMGV
                     VGLMNSFANGALGAIMQTKIPPEMQGRIFTVIGSLSSAASPLGMLLAAPVAEQLGIRA
                     WYLIAGIFCAAMGLYGFIERDVYTLEDQEPGGKLAAAPAQVEEPL"
     gene            complement(5006..5689)
                     /locus_tag="PKL60_02490"
     CDS             complement(5006..5689)
                     /locus_tag="PKL60_02490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNW95037.1"
                     /translation="MSAPRSPAPPLDLSRVKALLFDVDGTLADSDNAAADRFARRLRL
                     AVGERRAGQLSRQLVMAAESPGNWFLELADRLSMDHLIARFLERRAARQSARVLPAIN
                     GVPAMLASLHDRFPMAVVSARNNLTTLNFLRGHDLEQYFSVVVTSQTCPRTKPFPDPL
                     FFAAQSLGVPIENCLMIGDTTPDVRAALAAGAQSLSVLCGFGTRKELERAGTQHILAE
                     TADLSRFLA"
     gene            complement(5704..7083)
                     /locus_tag="PKL60_02495"
     CDS             complement(5704..7083)
                     /locus_tag="PKL60_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95038.1"
                     /translation="MTPSTKKNLIIVLIVVLVLCLCAAALALLGGIALFRNFSPTTAA
                     QSTPEPLVLTATALPQTTPASPEDAVSASMDEIEAQVSAIRGLELETPIPRKLLTEEE
                     LQAAVENDFFKDYTPEDAASDARVLSLLGLLPRDFDLLSFYRQLYSEQIAGYYDDEVK
                     AMFVVKNSGFTGVERDTYAHEFTHALQDAAFDFNGALNYTDEACQEDSERCAALQSLI
                     EGDATLTESLWLQNHATPQDIRDLQNFYANYESPVYDSAPAYMQEDFLFAYQQGLAFV
                     QTLYDSGSYAAVDAAYTTSQPVSTEQIMHPNRYPADIPAKVELPDLSAALGSGWEESE
                     RNVMGEWYTWLILARGADAESRLVDAKASAAAEGWGGDTYVVLENTASAESALLVRYL
                     WDGSADSAEAKLAFESCLSLRLGRPDQAGIYAQDGIYSALRQDSQFGFTWLIADSRAA
                     LETLLGELY"
     assembly_gap    7337..7436
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7494..9158)
                     /locus_tag="PKL60_02500"
     CDS             complement(7494..9158)
                     /locus_tag="PKL60_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNW95039.1"
                     /translation="MTPLLIAGLTAGALLLIILAVILIRTLGFPLESEPTEALALMDV
                     DGEAVARRIGLAVQCRTISRSDPTLTDPRSFTALHELLRTLYPQLHEKLDLELVNDRS
                     LLYTWRGSDPALDPICFAAHQDVVPADEAPEAGWTHPPFAGELADGYVWGRGTLDCKG
                     TLIGLCEAATNLLKSGFSPRRTIYLAFGADEEVSGLTGARAIAELLKERGVRLAFLLD
                     EGSAVTKGSAMGVNSPIGQIGISEKGYLSLKLKAKVKGGHSATPPKQTAVGALALAIA
                     VLEANPFPQNLDVMSFIASYLGKDLPFLQRMQYANTWLFGGALKRKLAETPLTNALTR
                     TTIAPTIIKAGSAENVLPAEAEALLNLRIMPGETLREVYERVNDLVGDENLEVLPAHG
                     ETLENEHAWNPTPVADVDSPQYQRLAELVRATFPGAIVVPFMMAGATDARHYAAVCQN
                     AMRFSPLFLTQDESQTMHAVDERLSFINASRMVAFYEELMRQESSLLDEEEERGEVEA
                     PLKVKSPSRKERRAAAGEEMQEAPAEPAELPLDDEPLVVKPLRKEE"
     gene            complement(9754..12753)
                     /locus_tag="PKL60_02505"
     CDS             complement(9754..12753)
                     /locus_tag="PKL60_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNW95040.1"
                     /translation="MKKFVPILVLLALLFMPLADSAQALSARSPAPAILPQAGAGTEV
                     STGADPGNNGQQIQSLSGSYVAFDPSAGGESSYLPGVSQTLCFRSESFTTDWEYVYNN
                     WLKFPSNWTVSNVYVAGTPSCTSGAGWGAFGWSFATAPYEVNITHTRSQATTDHCVAN
                     YCVVVTPETAADPAPVSWYFDGDGYGGTPHNPCSSDGYTPAGQLPCDESVNAPANVPF
                     GGTGFFFYPAEIHVSGCHGDEQIHEVHLANFTGAQVNVSLSYDADAGVGFSGPTNLTI
                     PDESTESFNVSLNPHVCLEDGEITATIDAIAGSYTDSFTIYKEINSTREGWQQLATNP
                     TTKMDNVLAAYSGKLWQITGYGSMEVSNYDPLTDTWTTLASSAPPFGMNYARSGCQHE
                     NKVFMYGDSTTGGFTGLWSYNMDTNGWTAETPGGTPPASTGIWAPAWTYDPAAHLCYL
                     TGGATAPGTGNLTSVYVYDPVGNAWLTPLPNFNTPRDFHAAFFYENPTTTHKLLCVAG
                     GNFSSTGLTSTQCFDFTTGLWNAENADIPALPASLWGMGYAMTDAGTGNDLWLVNGVD
                     AAGELFNISWRYNTAAGVWEDKGPLPGAMTYRTSAATLDNMVYITGGSTGGFSPSGYT
                     HRYLVEECEECIVPEITKDAAPVVLPGETITYTLTISQLHEDTALVEDTLPAGIEYVP
                     GSLTVSPDLGEYEYETSTRTIRWLYDPSENKTVLWEPVSTDGTATADGALSMVGTSGA
                     QASLAQPTAQTESDLLWDQPLSTVNTDAIVDQDFPDEPSFSSFLADDFVAGTPWVVDG
                     FYIPGDGWNGFSTLLNATQLTFMIYEDDMGVPAGDPSGRGDLPFWALSIPPTDSQLTI
                     TSGFSGMPSNVQLDLDSAVLLPAGHYWFIFYSTMFYSSYGQYGRQPSDTTSGYTAQIV
                     NPGNGFGFGTEWQSWDVIGILQQDIAFSIYGTSIQSVVITFEAEVLELNKTIVNESWL
                     YYGDENRSDDAITFTGYGMYLPIAMKQ"
     gene            13074..13146
                     /locus_tag="PKL60_02510"
     tRNA            13074..13146
                     /locus_tag="PKL60_02510"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13107..13109,aa:Phe,seq:gaa)
     gene            13242..14375
                     /locus_tag="PKL60_02515"
     CDS             13242..14375
                     /locus_tag="PKL60_02515"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNW95041.1"
                     /translation="MTKRIGLLTAGSDCAGMNAAIRGIGKAVSTEKNLDLIGFRDGFH
                     GLVEDRTMDLSGGVLSGILTLGGTILGISRNIPQAMPGANGSADMTQNAVDTYKRHKL
                     DALVCIGGKETIKAGLHLSKAGLNIVVLPKAADNDIPGTDKAIGFDTAREIATQAIDR
                     LHSSANATHRIMIVELAGVSAGWLTLGAGLAAGADVILIPEFPYDFGLISAAIGARQK
                     AGRNFSLVAVAENARSQELVEFMERAARKKAGQEAASDSAVSAEMERIGKSYSGETML
                     LARRLEDETGLQSRITILGALVRGGTPTASDRLLATQLAEWSVLMVETGQFGHMAVRM
                     DDAMSSTPLEDVVDKHKLVPADHGWIRGACQVGTCLGVKNPGL"
     gene            complement(14569..15252)
                     /locus_tag="PKL60_02520"
     CDS             complement(14569..15252)
                     /locus_tag="PKL60_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNW95042.1"
                     /translation="MPTKTFLNLSETKRAQIENAAVREFARNGLRGASVNALVAGSGI
                     AKGSYYRYFSGMEDVFQHILSIARQKKLALANTLNSSNGRDMFTYLRWLFQVEILFEL
                     REPELSAIEHHAFLESLNSSAPVDENAPLIGGPSFFNEFLAQGILADDVATWVDTKLG
                     AHLLGAAYHLLTHQLVVRLGESAETLRSGKADIREDPLAQSLLDNLMDLLEAGLAREP
                     AIRKDFYSK"
     gene            15364..15852
                     /locus_tag="PKL60_02525"
     CDS             15364..15852
                     /locus_tag="PKL60_02525"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNW95043.1"
                     /translation="MDHIYSPWRKVYFQRDKTDPTCVFCAAQETEDSPENLIVHRGER
                     TYVILNLYPYSNGHLMVVPYQHCQELAELPKETLEEMICTASKAVALLESIYHPQGFN
                     LGMNIGSPAGAGIAGHLHMHVVPRWSGDANFISVIGQTRVLPETLEETYERLKAAWNQ
                     EA"
     gene            complement(15885..16379)
                     /locus_tag="PKL60_02530"
     CDS             complement(15885..16379)
                     /locus_tag="PKL60_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95044.1"
                     /translation="MMGKRQQIAFGAALFLIGAALFARPYVPALEPFLSWPLLLVPLG
                     LLGVVCSAILEQGGWLISAALITGVGANLWAERALDGGTWASMAAFLGLGLMLASVFD
                     PDEKDAWRPGLILIVVSAVMFLLLGGKKYLPWPQFSGFWPLAIVLVGLAYILAGLTRK
                     KKDS"
     gene            complement(16376..17140)
                     /locus_tag="PKL60_02535"
     CDS             complement(16376..17140)
                     /locus_tag="PKL60_02535"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNW95045.1"
                     /translation="MNKKSTIAFLVALLALLTLACGLPGNLAGPKTPVVEKGSGKVVS
                     SEPAVSEFLTIKLTGGGELRLVQGSEYKLVIEAEDNILPHLTAEMDGSTLNLGYEESL
                     WKERYLPTEPIIYTVTFTNLEQLILLGGAKITCGALDLPSFRLNLNGGVDVTLQNVRA
                     DTLELQLDGGANVNISGEVGYQSLLLNGAGAYSAQDLKSRDAKVVINGAGDAKVWATD
                     TLDIALNGFGSVSYYGSPQVTQSLVGLGTIKSLGEK"
     gene            complement(17313..19526)
                     /locus_tag="PKL60_02540"
     CDS             complement(17313..19526)
                     /locus_tag="PKL60_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNW95046.1"
                     /translation="MAKRTLKRQLNLTQVIMLGTAGTLGSGIFILTGHAAAVAGPATI
                     LAVIIAGILSFSIALNYCELATTYPETGGAMTYVREAWGKGLLAFLVGSMDSISSTFY
                     CALSAVGFAYSLSVFIPGLPIVPVAIAAILAFVILNILGVTNVGNIQIVMGILLLSAF
                     TFYIVGGFLLPNGFNAETFLNGGKFFIGDNFWQNLTSILRTIALIYALYVGFEVIADD
                     AEEVKNPTKNIPTAIIVSLIIITLVYSLSVTVALGTTPWQQLAGSETALSDTVRKFSP
                     MLGVAIIGAAGMIGALTSVNSSMLSATRESFTLSRDGAWPAVLSRLNKARVPFMAILV
                     IGLISMFITGIGLVNFLSYITSAGYLFVLFFSNMAMIKLRSKFPYIHRPYKVPFFPLT
                     PIVASLTCLIVICFSELRALAFTAGILLLFALYYYARIGFASWQEAHTRSLSPGRYRL
                     LLPVTDFSGLDTLMKIGASLAEAKSDMNMCMLLVLNRSAERTEQVVEHFRQTRQYVME
                     KFIHYAVERNVPTYSKTVTASSLSEGIINEIKMDNNVRLVLLRMPHESAGQKLINNTV
                     QELFRENIVNIGVLYDKGLPQLQNILVPVGGGYHCRLAIQLAHELAAINNGQVDFLRV
                     VPSEIVPEEYEDQMAYLQEIVMSELNGIPTDASLNLDQSDSAAESIIRHARLGKSDLV
                     IIGSSEIPQEDELFGEIVEDVAARVPCSTLVIRQHESQAASWLRRQLKSMEKSAE"
     gene            19725..22676
                     /locus_tag="PKL60_02545"
     CDS             19725..22676
                     /locus_tag="PKL60_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNW95047.1"
                     /translation="MDPKNPLENHIVMADQVWHARGVEDALAGLETNAEQGLTLAEVQ
                     ERQMRCGFNELQEKALPSFWELLGRQLKDFIVILLMVAALVSALLGDWIEAGIILLIV
                     VLNAILGVVQESKAEASLAALKKMAAPECQVLREGRRLTVPARELVPGDIVFVEAGNY
                     VPADIRLLESVNLRVEEAALTGESVPVQKNASAVLPDNAALGDRKNMLFSATLVNYGR
                     GHGVVTATGMNTQIGRIAGMLQSLDEGKTPLQERLDKLGKTLGLFALLICGLVFVLGL
                     ARSGVFAQGFTRASSGAIVESFMVAVGLAIAAVPEGLSAVVTISLALGMREMIKRHAL
                     IRKLQAVETLGSVSVICSDKTGTLTENRMTVTVVDMANHVLDFTHEAENGVIRGLQEV
                     LRVEAPDEDQVRMLKEHPTLLHILTGGTLCSDAVIEPHPEKEDHIVMIGDPTEGALVV
                     AAARMGLSKKLLSEALPREAELPFDSERKRMTTAHRLPASREELPEALRDLWNWEASE
                     EARYLAYTKGSVDGLLNIADSLFVDGQLVPLEEEWRARIEARNANLASQGVRVLGLAY
                     RLLPELPENLEAAHVEKDMTFVGMVGMIDPARPEVKDAVALCKSAGIRPIMITGDHPL
                     TARHIAEDLGIASNGKALTGADVEKLSDAELENAVREVSIFARVSPEHKLRIVQALQT
                     NGEVVAMTGDGVNDAPALKKADIGVAMGITGTDVAKGTADMVLTDDNYASIVAAVEQG
                     RVIYANIRKFVFFLLSSNLAEIMIIFLATLLGLPMPLTVIQLLLLNLLTDGLPALALA
                     VEKGDPDVMQQPPRPKNEPVINKSMTTGLVIQTIFQTAAVLGAFLLGLWRHPGAALPA
                     GANPFLTLLRFDWAGVDVLTAETMAFVTLSLCELFRAYTVRSERVSVFKLGVFSNPAM
                     QPAVLVSLLVMLLVVNVPFLQPVFNTHFLAAQEWMAVIGLGLVPAVVEEITKLFLRKA
                     DGKKKNQGQAE"
     gene            22845..23939
                     /gene="tal"
                     /locus_tag="PKL60_02550"
     CDS             22845..23939
                     /gene="tal"
                     /locus_tag="PKL60_02550"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNW95048.1"
                     /translation="MNSTQRLWALGQSVWYDNIQRSLLRNGEMAGMIAREEIRGVTSN
                     PSIFMNAIAKSSDYNESLKPLGDPALSAEEVFFRLAIEDIQEAADLFLPLYQSSGGAD
                     GYVSLEVSPFLADKSAETLAQAEALWGRVKRPNLMIKIPATKAGLPAIRAATAAGIPV
                     NVTLIFSRERYGEVMEAYLSGLEQRAQAGLPLEGTASVASFFISRVDSHIDPRLHTIG
                     SAEALALAGKAAIANARLAYAQFLQVFGSGRFARLEALGARKQRPLWASTSTKNPAYR
                     DVLYVEKLIGADTVNTMPPQTLAAFLDHGQVRESITEDLEEARILFDRLEALGISMPQ
                     VTAQLEMEGVQAFADAFSVLLNVLEKRRKA"
     gene            complement(24018..25475)
                     /locus_tag="PKL60_02555"
     CDS             complement(24018..25475)
                     /locus_tag="PKL60_02555"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HNW95049.1"
                     /translation="MKTLKVLFLAAEADPLVKVGGLGDVAGSLPQALRALPAEDTNGV
                     RADVRLLLPYHDAVRQKLPTAEFLFSFPVESKTGALNAEVYYSEVNGVPVYLIGGEPI
                     SNSPVYSPDSAADGRKYVFFSAAALGFIDRLEWKPDIVHANDWHTALVVYLLKLRRKA
                     SPAAAHPKTVFTMHNLPFMGAGTEAAFDNYGIPPSRYSRVPWWGKKFPLPLAIQTADR
                     VTTVSETYAQEILTPEFGCGLEKLLQTRHKVVSGIVNGLDMDAWDPSADQRLPASFSA
                     ETLDARAANKTALAQEFDLDPDPNLPLLVLVSRMDQQKGVDLAVQGLASLAKRRWQAI
                     LLGTGNPELEEACRKLEKAHPRRVRAAIRFDVNLSRRMYAGGDILLMPSRYEPCGLAQ
                     MMAMRYGCVPLARATGGLQDTIRDVSIHPESGTGFLFKPATAADFAGALTRALDLFPD
                     RGAWQAMQRRGMRQDFSWSRSALKYLRLYQGLVAK"
     gene            complement(25486..>26755)
                     /gene="pgi"
                     /locus_tag="PKL60_02560"
     CDS             complement(25486..>26755)
                     /gene="pgi"
                     /locus_tag="PKL60_02560"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNW95050.1"
                     /translation="RFSESVRSGEWRGFTGKPITDIVNIGIGGSDLGPKMVCAALKPY
                     GKHGLRVHFVSNVDGTDIAETLKLLNPETALFLIASKTFTTQETMTNAHTARDWFLAR
                     SGEERYIAQHFAAMSTNTQAVRAFGIDPANMFEFWDWVGGRYSLWSAIGLPIALFVGM
                     DNFEALLRGAYAADQHFRSAPFSQNIPVLMALLGIWYNNFFGAQTHAILPYEQYLEYF
                     PAYFQQGDMESNGKGVSRTGEFLRYSTGPIIWGQPGTNGQHAFFQLIHQGTKLIPCDF
                     LAGVETQNPLGSHHAILLSNFLAQTEALMKGRTPEEARAELAAQGFAGAELELLVRAK
                     TFPGNRPTNSFLYQKLTPETLGTLIALYEHKIFTQGVIWRINSFDQMGVELGKQLAKA
                     ILPELEGAALVNTHDSSTNGLINAIKSRRE"
BASE COUNT         5437 a         7158 c         8562 g         5488 t
ORIGIN      
        1 tttatgagga aacttatcaa tttccaagcc gtagaggctg gattctcagg cctcaggccc
       61 ccagcaggtc tggcagcagc gccaggcgcg ggatagtgcg gaagccgtct tgcgggagga
      121 gcgagaccaa tacggtgtgc atgcccaggc tggaggcagc ggccacgttc tccaggcggt
      181 catccaccat cagcacttcg cgcgggtctg ggctgccggc cagttcgagc gcgatgtggt
      241 aggcttccgg ctcgggcttg cagtagggct gcacagccac gacgtcaata atgccctcaa
      301 agcagctttc caggtccatc agcctgagga cgttgcgggc atgggattca caggcgttgg
      361 tgaagattaa cttcggcagc gcgatgcggt ccagcgccga gcgcagcgcg gggtccggac
      421 tgagcagggc ggaaaggtcc acgtcgtgga cgtaatcgag gtaagctttg gcgttgatac
      481 cgcgttcgat ttgcaggccg cgcagggtgg tgccgtagcg gttgaacagc cggtcgcgca
      541 tggctggaat ttcatcctcg gggatgccaa gaaaatcgcg catatagaga aacatacgct
      601 tcatcaccaa aggccagacc tctaccgtag gcgggtaaac cgtgtcgtcc aggtcaatca
      661 ggagcgtgga gattttcacg ggcgttccac ccagcggcta aggtttccgg gcgcctggta
      721 ggcttgcatt ttttccagca gggattgggg gtccgcttcg cacagcataa gctggcggtg
      781 ttcggggtaa atgaagtgtt cggcgatggc atgatccacc aaggccagca gcgggtcaaa
      841 atacccggcg atgttgagca ggccaatcgg tttggtgtgc aggccgattt gcgcccaggt
      901 cagcgcctca aacagctcat caaaagtgcc caacccgccg ggcagcgcga tgtaggcatc
      961 cgcgagctcg gacatgcggg ctttgcgcgc gtgcaagtcc ggcaggactt ccatgcggct
     1021 gaggccggca tgcgcgagcc tgggggtgtt caggctttcg ttaatcacgc ccacgaccaa
     1081 gccgccgcgc gccagcgcgc cgtcggcggc tgcgcccatc aggccggttt ttccgccgcc
     1141 aaagacgagc gtaatgccgc gttctgccag agccgcgcca agctgccagg cgccctgcag
     1201 atatgcctgc cgcagatggt ccgcggaacc ggagaagacc gtgatggttt gaaggggttt
     1261 tgagggcatt tatcatcctc gctggagcgt cgtcagccta ttatacccac aggcgcgcag
     1321 ccgggggaaa gcggcgcgcg ccgtggttat tgtgcccggc gcatgttcct gagttcgcgt
     1381 actttgtcct tttccgcggg ggtgaagtgt tggggcaggt gaatttcgac agtggcgtac
     1441 aggttgccgc gggtctgcgg gttcttgagg ctgggcatgc ccagcccttt caggcgaaat
     1501 accttgccgg tgtcagtgtt ttcggggatg gtcaaccgca ccgcgccagt gagcgtgggg
     1561 acgctgacct cgccgcccaa aaccgcggta tagaagtcca cggggacgcg ggtgtacagg
     1621 tcgctgcctt tccgggtgaa aattgcatgc ggaaggacca cgatgcgcag gaacaagtcc
     1681 gcgccgccgg cctggccgct caaacgcacg cgcgagccgg tatccacgcc gggaggaatg
     1741 ctggcttcga tgcgcttgcc gtcctcaaac tgcagcaggc gtttggtgcc gctgaaagct
     1801 tcttctagcg tgatttccac ttcgtgctgc acatcctggg gggctgcgga ctggcggcgg
     1861 cgggcagagg ccgggttggt ctggcgggag ctctgggtgt tgaaaccgcc gctaaaaccg
     1921 ccaaacaagg tttcgaagaa atccgagaaa ccgccaccca tgccgccgaa catctggttc
     1981 aattcctcct gggagacggt gcggtagctc gtgccgcccg cgggctgcga gcgccagggc
     2041 ccccagttga aatcttccgg actgccgcca gtggagcggt actgctgcca ctgtgaaccg
     2101 aactggtcgt acttctggcg cttttcgggg tcggaaagaa cctggtaggc ttcattaatt
     2161 tccttgaact tttcttccgc ttcgggcttg ccggggttca tgtccgggtg atacttgcgc
     2221 gccagacgcc ggtaggcgga cttgatatcc gcttccttgg cggtcttatc cagaccgagc
     2281 gttttgtaat aatccttata ttccataata tgaagcctaa ttctcctgca gtttaatgaa
     2341 tttctatacc atactcacgg actgtttgat ttttgttaat tcgcagagag ctctagccct
     2401 tggagagagc ttccgtagat tccgcgggca tgcgcgggca gcagctcggc gatacggctc
     2461 atgatttccc agccggcagc ttttacctct tccctgccag ggattttatc caggctgccg
     2521 gctgcgagcg gaccaaaagg cttgccgatg cgggtgatga ttcgagcacg gcgcttctta
     2581 cgcgtaagag cgccgaacaa gtcccattcg tcctcgctgt aagtgccgat aggaagaatg
     2641 ggcacccccg agcgcagcgc caggaaggag gccccgggca gcggtttgcc aagcgggagg
     2701 cggtgcacat gcccttccgg gaaaattgcc aggacgccgc cctgcgccag aactgcttcc
     2761 nnnnnnnnnn ggccgcgctg gcggccttct cctcgccgat caaccaggag tggttcatcc
     2821 tgcgccagga ccgcttctga agcccgcatg gcttccaggt tggcggtgcc ctgcaggatt
     2881 ttgaacgtac cccacaggtc cgggaggaat ttcatggcgg cgggggcgtt gggcataaca
     2941 gcatcgttaa taaattccag caggtaatcc gtggagtgaa tcggaccgat gctatccaga
     3001 aagtggaaat gattcccaat gataagcagc ggaccgccct ttggcagatt ctcctgaccc
     3061 tcgatgcggt agttcgtgat taaggaggct gccagcgcga tgccgctttt tagcgcgcgg
     3121 cggatgaaac gccggcgggg atagggatac ttgagtttgt attcgtgtaa gttttttcgc
     3181 gagctcatca ggcacctcag gaacgggact tgttctaatt ttactctgtt aatgagagag
     3241 cataaacttg ttttgtacga gcgctggatt acgcgggttc gcattggcgg ggggcagcag
     3301 cgggatgatt tcgggtgcga gcgaagtatc catgagtgat gttcataatg aaggcccgta
     3361 atctgattgg tatgcttggc ccctgcggct ggagacacgc atgccgaagg ttaagctggc
     3421 gcgtgcagcg agggtgagct gagggataaa aagaccatca tccgccttct ccccggagaa
     3481 ggttagcgca ttcctgtcct cccctacgac caaatctcaa ggcgagttaa ccggcagcgt
     3541 gaagcacgca agctgggcgc acctggatgc aacggctcgg gatgtgaaaa ctcctttaca
     3601 ccaacaggga aagctctata attgcacttt atctctcatt catcctggat ggtgcagtaa
     3661 tgaccgtgga aaacagcgac agccaaatca ctcaagacct gcccgcgcac tggctgcgga
     3721 attacctgcc gatgtgggtc gggcaggcgg tatcgctgtt gggcagtggg ttggtgcagt
     3781 tcgcgctggt ctggtacatg accgaaaaga ccggctccgc caccgtgttg gctatggcga
     3841 cctttgtagc actgctcccg cgcgtgcttt tgggcccatt tgccggcgct ttggtggacc
     3901 gattgaaccg caaggccatt atgattatct ccgacagcgc ggtggcgctg gctaccttcg
     3961 cgctggtggt atcgttccac ctggggatta cgcaaatctg gcatatctat gcggcgctgt
     4021 ttgtgcgctc gctgtgcggc tcatttcaag gcccagcctt gcaggcttcc gtcagcctga
     4081 tggtgccgcg cgaacatttc gcgcgcctgg gggggattaa tcaggccatg tttggagtga
     4141 ttgatatcgt cagcccgccg ctgggcgcgc tgctgatttc tgtgatgact atgcaatccg
     4201 tcctggcgat agacctggtg acggccgcga ttgccatcgc cttgttggcg ttcctggtgc
     4261 atgtgccgca gcccgcaagc gtatcgacca cggaagcgct gacgcccaaa cgggtgctcg
     4321 cagacgtccg gaacggcttg aagtatgcgg tgggttggtc aggtatgttc aaggttatcg
     4381 tgatggcgtc gctcatcaac atgcttgcat cgccggcgtt ttcgctgctg cccctgatgg
     4441 tgaccaaggt ctacggcaag ggtgcggcgg acgttgcctg gctggaatcc gctctgggac
     4501 tcggcgtggt ggcaggcggg ctgctgctgg gggtatgggg cggctttaag cgcaaaacgg
     4561 cgacgacctt gacagggctg cttgggatgg gcgtgggcat cacgattctg ggcctgacgg
     4621 accgcagtac ctatatgctg gcagtgggaa tgatgggcgt ggtgggttta atgaactcct
     4681 tcgcgaacgg agcattaggc gcgattatgc agacgaaaat cccgccggaa atgcagggac
     4741 gtattttcac ggtcatcggc agtctttctt ccgcggcctc gccgttgggt atgctgctgg
     4801 cggcgccggt ggccgagcag ctgggcatcc gcgcctggta cctcatcgcg ggcatttttt
     4861 gcgcggcaat gggcttatac ggcttcatcg agcgggatgt gtataccctg gaggaccagg
     4921 agccgggcgg aaagctggcg gctgctcctg cgcaggtgga agagccgctg taaacggggc
     4981 tcttgtccgt gtaatgggag ggaagtcagg ccaggaaccg ggacaggtcg gcagtttcgg
     5041 ccaggatgtg ctgcgtcccc gcgcgttcca gttcctttcg ggttccaaaa ccgcacagga
     5101 ctgaaagcga ctgcgcgccg gcggcaagcg ccgcgcgcac atccggcgtg gtatctccaa
     5161 tcatcagaca gttttcaatc ggtacgccca aagattgcgc ggcaaagaac agcgggtccg
     5221 ggaagggctt ggtgcgcgga caggtctggg aagtgaccac cacgctaaag tattgctcca
     5281 ggtcatgtcc ccgcaggaag ttgagcgtgg tcaggttatt gcgcgctgag acgaccgcca
     5341 tcggaaaacg gtcatgcaag cttgccagca tagcgggcac gccgttgatg gcggggagaa
     5401 cgcgcgcgct ttggcgggcg gcgcggcgct ccaagaaacg ggcaatcaag tggtccatgg
     5461 acaggcggtc agccagttcc aagaaccagt tgcccgggct ttcggctgcc atcaccagct
     5521 ggcgcgagag ctgcccagcc cggcgctcgc caaccgcaag gcgcaggcgc ctggcaaagc
     5581 ggtccgccgc ggcgttgtcg ctgtcggcca gcgtgccgtc cacgtcaaaa agcagggctt
     5641 tcacccggga aaggtccagc ggaggggcgg ggctgcgcgg agcgctcatc gcggtccctg
     5701 gtactaataa agctcgccga gcagcgtttc gagcgcggcg cggctgtcgg caatcagcca
     5761 ggtaaagccg aattgcgagt cctggcggag cgcggaatat atgccgtctt gggcgtagat
     5821 cccggcctgg tcagggcggc ccaggcgcag gctcaggcag ctttcaaagg caagcttggc
     5881 ttccgcagaa tcagcggagc cgtcccagag ataacgcacc agaagggcgg actcggcgga
     5941 cgcagtgttt tccagcacaa cataggtatc gccgccccag ccttcagcgg cagcggaggc
     6001 tttcgcgtcc accagtcggc tttcggcgtc agcgccgcgg gcgaggatga gccaggtata
     6061 ccattcgccc atcacgttgc gttcgctctc ctcccagccg gaaccgagcg cggcgctcag
     6121 gtctggaagc tccaccttgg caggaatatc ggctgggtag cggttgggat gcatgatttg
     6181 ctcggttgag acgggttggg aagtggtata ggccgcgtcc acagccgcgt aactgccgct
     6241 gtcgtagagg gtttggacga aggcgagccc ctgctgatag gcgaagagga agtcctcctg
     6301 catgtaggcc ggcgcggagt cgtagaccgg gctttcgtag ttcgcgtaga agttctgcag
     6361 gtcgcggata tcctgagggg tggcgtggtt ttgcagccag aggctctcgg tcagggtcgc
     6421 gtcaccttcg atgagagact gcagcgcggc gcagcgctcg gaatcttcct ggcaggcttc
     6481 gtcggtgtaa tttagcgcgc cgttgaagtc aaaggcggca tcttgcaggg catgggtgaa
     6541 ctcatgcgcg taggtgtcgc gttctacgcc ggtgaagccg ctgtttttca cgacgaacat
     6601 ggctttgact tcgtcgtcgt aatagcccgc gatttgttcg ctgtaaagct ggcgatagaa
     6661 actgagcagg tcgaaatcgc gcgggaggag gccaaggagc gagagtacac gcgcgtctga
     6721 ggcggcgtct tcgggagtgt agtccttgaa gaagtcgttt tcaaccgccg cctggagttc
     6781 ttcttcggtc agcagtttgc gggggatggg ggtttcgagt tcgagaccgc ggattgcgga
     6841 gacctgcgcc tcgatttcat ccatggaggc gctgacggcg tcttcagggg atgcgggggt
     6901 ggtttgcggg agggcggtgg cggtgaggac cagcggttcc ggcgtggact gggctgcggt
     6961 tgtcggggaa aagtttctga agagggctat gccgcccagg agggcgagcg cggccgcgca
     7021 caggcacaag accagcacga caataaggac gatgataagg ttttttttgg tggagggggt
     7081 cataggtttt cctttgcgcc taagtatatc attcgggggc ggcaaggatt gcggggtaga
     7141 gggctttcaa aggaaaagtt aaggctggcg gaacagcgcg gcatgtagct tgggcgcagc
     7201 gacgcaggag cggaacccaa cggtgaatcc catggctagc aatccggttt tgtcaggtta
     7261 cgctggcgct ccacccaagc tacgactcac aaccgggatt tcatcccaat catccaaaag
     7321 tggcagcttg gtctgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacga
     7441 ctcacaaccg ggatttcatc ccaatcatcc aaaagtggca gcttggtctg ctgttattcc
     7501 tctttccgca gcggcttcac gaccagcggc tcgtcatcca agggcagttc cgcgggttcc
     7561 gccggcgctt cctgcatttc ctccccggcg gcggctctgc gctctttgcg ggagggtgac
     7621 ttgaccttca gcggcgcttc cacttctccc ctttcttcct cctcatcgag caagctgctt
     7681 tcctggcgca ttaactcctc atagaacgcg accatgcggc tggcgttgat aaaggacagg
     7741 cgttcatcca cggcatgcat ggtctggctt tcgtcctggg tcaggaagag cggcgagaag
     7801 cgcatggcgt tttgacacac ggctgcgtag tggcgcgcgt ccgtggcgcc ggccatcata
     7861 aagggcacga ctatcgcgcc ggggaaagta gcgcgcacca gttctgccag gcgctggtac
     7921 tgcggggagt ccacgtccgc cacaggggtg gggttccagg cgtgttcgtt ttcgagggtc
     7981 tcgccgtggg cgggcagcac ctcgaggttt tcatccccca ccaggtcgtt cacgcgttca
     8041 tagacttcgc gcagggtttc accgggcatg atgcgcaggt taaggagagc ttcggcttcc
     8101 gctgggagca cattttcggc gctgccagct tttatgatgg tgggggcgat tgtggtgcgg
     8161 gtgagcgcgt tggtgagcgg cgtttctgcc agcttgcgct ttagcgcgcc gccaaacagc
     8221 caggtgttgg cgtactgcat gcgctggagg aagggcaggt ctttgcccag gtagctggcg
     8281 atgaagctca tcacgtccag gttctggggg aaagggttgg cttccaacac cgcaatggcc
     8341 agggcaagcg ccccgacagc ggtttgtttg ggcggggtgg cggaatgtcc gcctttgact
     8401 ttggctttga gtttgagcga gagatagcct ttctcgctaa tgcctatttg accgatgggg
     8461 ctgttgacgc ccatggcgct gcccttggtg acggcgctgc cttcgtccaa caggaaagcc
     8521 agccgcacgc cgcgctcttt caggagctcc gcgatggctc gggcgccggt aagcccggag
     8581 acttcctcat ccgcgccgaa ggccaggtag atggtgcggc ggggggagaa gccggatttg
     8641 agcaagttgg tggcggcttc gcacaggcca atcagtgtgc ctttgcagtc cagcgtgccg
     8701 cgcccccaca catacccgtc ggcaagctcc ccggcgaaag gcgggtgcgt ccagccggct
     8761 tcgggggcct cgtccgccgg gacgacatcc tgatgcgcgg cgaagcaaat cgggtcaagc
     8821 gcggggtcag agccgcgcca ggtgtagagc agcgagcggt cattgaccag ttcgaggtcc
     8881 aacttctcat gcagctgcgg gtagagcgtg cgcagcagct cgtgcagggc ggtgaaggac
     8941 cgcgggtccg tgagggtggg gtcgctgcgg gagatcgtgc ggcactgcac cgccagaccg
     9001 atgcgccggg cgacggcttc gccgtcaacg tccatgagcg cgagggcttc ggtgggttcg
     9061 ctttcaagcg ggaagccaag cgtgcggatg aggatgacag ccaggatgat gagcaagagt
     9121 gcgccggctg taaggcctgc aatcagaaga ggggtcatac gtacctccgg tgtgatttgt
     9181 agtggtaatg cttctgcttt attgtaccgg aaaataggac ggagggaaaa ttcgagcccg
     9241 ggagcacggg tttttgtggg gatttttttg gggggtgggc gtggtgagga agggttgtgt
     9301 gagccgtgta cagctggaga aaagaacagg caggttggat gaagaaacat tcgtcatcct
     9361 gattgcagtg aaagatttca cttttagcag gggtagaaag gcgcataggc agacaggcaa
     9421 agaggtagac aggcagacag ttcggttgcc acgtccctta ttgtgattcc cggcatgcgt
     9481 gcgttcttag ggtgagggct cgcaacgacg attcaacagg cacctgagcg cagcgaaggc
     9541 tctttttttt cgactgacca gatgctttgc ctgcggcaag tgcccttgag ggtatcagga
     9601 ggataagacc cttgtccctc tgagcgccag cgaagagtct ttcttttcaa tcgcgattgg
     9661 cgctttgagg aacgcaaacc gccctgcatc ctgcaaggac aaaaaagggg ctgccagagg
     9721 gcagccccga tgagcattga caaagcggga atcttactgt ttcatggcga taggcaaata
     9781 catcccgtat ccagtgaagg tgatagcgtc atctgaacga ttttcatcgc cgtagtataa
     9841 ccagctttcg ttcactatgg tcttattcag ctctaatacc tcggcctcaa aagtgatgac
     9901 cactgactgg attgatgtac cgtagatgct gaaggcaata tcctgctgca ggattccaat
     9961 aacatcccaa gactgccatt cggtgccaaa tccaaatcca ttgccgggat tgacgatctg
    10021 ggctgtgtaa ccactggtcg tgtctgaagg ctgccttcca tattgcccgt aagaactgta
    10081 gaacatcgtt gagtagaaaa taaaccagta atgcccggct ggcagcagga cggccgaatc
    10141 taggtcgagc tgcacattgg agggcattcc actgaatcca ctggtaatgg tcagctgcga
    10201 atccgtgggt ggaatactca gcgcccagaa gggtagatcg ccgcgcccgg acgggtcgcc
    10261 ggcaggaacg cccatgtcat cctcgtaaat catgaaagtc agttgcgtgg cattcagtag
    10321 ggtgctaaag ccgttccagc cgtcacccgg gatgtagaaa ccgtccacga cccacggtgt
    10381 gcctgcgaca aaatcatccg caaggaagga tgagaaggat ggttcatctg gaaagtcctg
    10441 atcgacaata gcgtcagtat tcaccgtgct gagcggctga tcccacagca ggtcggactc
    10501 ggtttgagca gttggctgcg caagagaagc ttgggcaccg gatgttccca ccatgctgag
    10561 ggctccatcg gctgtggcgg taccgtccgt tgagacaggt tcccaaagta cggttttatt
    10621 ttccgacggg tcgtagagcc acctgattgt gcgggtgctt gtttcgtatt catattcgcc
    10681 caaatcaggg cttaccgtga ggctgccagg tacgtattca atccctgccg gaagcgtatc
    10741 ctcgaccagg gcagtatcct catggagttg agagatggtg agagtgtacg taatcgtttc
    10801 gccgggcagc acaacggggg cggcatcctt ggtaatctcc gggacgatgc actcctcgca
    10861 ttcctcaacc aagtaccgat gcgtgtagcc cgagggagag aacccgcccg ttgaaccacc
    10921 ggtaatgtaa accatgttat ccaaggtcgc ggcggacgtg cggtaagtca tagctccagg
    10981 gagtgggccc ttatcttccc agacgccggc ggcggtattg tacctccagc tgatgttgaa
    11041 gagctcgcct gctgcgtcga cgccattgac cagccacaag tcattgccgg taccggcgtc
    11101 ggtcatcgcg taccccatgc cccataagct ggctggcagc gccgggatgt ctgcgttttc
    11161 ggcattccag aggcctgtcg tgaaatcaaa acattgggtc gaggtgagac ccgtactact
    11221 gaagttcccg cctgcgacgc acagcagctt gtgggtggtt gtggggttct catagaaaaa
    11281 ggcggcgtga aaatcgcgcg gggtgttgaa attgggcaaa ggcgtcagcc aggcgttgcc
    11341 gaccgggtcg tagacgtaaa cgctggtcag attcccggtg ccgggggcgg tcgcgccgcc
    11401 cgtcaggtaa cacaagtggg cagccgggtc ataagtccac gctggcgccc aaatccctgt
    11461 ggaagcaggc ggcgtaccgc caggcgtttc agctgtccaa ccgttggtat ccatgttgta
    11521 gctccacaag ccggtgaaac cgccagtagt tgaatcgcca tacatgaaca ccttgttctc
    11581 gtgctgacag ccggaacggg cgtaattcat cccgaagggc ggcgcggagg aagcaagggt
    11641 tgtccaggtg tccgtgagcg ggtcgtagtt gctgacttcc atagaaccat atccggtaat
    11701 ctgccaaagc ttgcctgagt acgctgccag gacattgtcc atcttggtgg taggattggt
    11761 tgccagctgc tgccatcctt cacgtgtgga attgatctct ttgtatatcg taaagctgtc
    11821 tgtataggac ccggcgatcg cgtcaatggt ggcagtgatt tcaccatcct ccaggcagac
    11881 atgcgggttg agcgaaacat tgaagctttc ggtgctctca tccggaattg tcaggttcgt
    11941 tggaccgcta aaacctaccc cggcatcggc gtcgtaggaa agcgaaacgt tcacttgcgc
    12001 gcctgtaaag ttggccaggt gcacttcgtg aatctgctca tcgccgtggc agcccgaaac
    12061 atgaatttcg gcaggataga agaagaaccc ggtgccgccg aaaggcacat tagctggcgc
    12121 attgacgctt tcatcacaag gcaattggcc ggcgggcgtg tacccatccg agctgcaggg
    12181 attatgcggg gtacccccgt agccatcccc atcaaaatac caggagactg gcgcgggatc
    12241 ggcagcagtt tcgggcgtca ccaccacgca gtaattggcg acgcagtggt ctgtggtcgc
    12301 ctgactgcgt gtatgggtga tgttaacttc gtatggtgcg gtggcaaagg accagccaaa
    12361 agctccccag cccgccccac tggtacagct tggggtcccg gcgacataga catttgaaac
    12421 agtccagttg ctggggaatt tcagccagtt attgtagacg tactcccaat ccgtcgtgaa
    12481 ggattcggag cgaaaacata aggtctgact gacgcccgga aggtagcttg attccccgcc
    12541 agcggacggg tcgaaggcga catagcttcc ggaaagggat tggatttgtt ggccgttgtt
    12601 tccggggtcc gcgccagtac tgacctcggt accggcgcca gcctgcggga gaatggcagg
    12661 cgcgggagaa cgggcggaga gcgcctgagc ggaatccgcc aaaggcatga acaaaagcgc
    12721 gagaagcaca agaatgggga cgaatttttt caattgcacg gtgagcctcc agaggaacaa
    12781 gaaatgctat tcagttgcag gttaagaatc agacaaatgg acggaacaaa gcgaacaaaa
    12841 ctacctgatc aacgggatca gaacgaccaa agagtttcag ggcggattgg aagaattctt
    12901 gaagttaacc taactataca atttatttga gcgctgcgca atagatattt tagaaataat
    12961 ccatttttgt tggaagattc aggataaaaa cagcctcagg ccagctcttt tcaggcggaa
    13021 tgcttgacag cgcgggtgct tacgggtaga attttgcaac tgttctaagg aacgccgagc
    13081 tagctcagtt ggtagagcat gcgactgaaa atcgcagtgt ccacagttcg attctgtggc
    13141 tcggcatgaa caccaaaaaa gtgccctcgg gggcactttt atttttatgc agatttccag
    13201 ctataatatt cctaacattg aatgcttcca gcgaggtaat tatgacaaaa cgcatcgggt
    13261 tattgactgc cggcagcgac tgcgcgggga tgaacgctgc tatccgcggc atcggaaaag
    13321 ccgtcagtac agaaaagaac ctggacctga ttggtttccg ggacggcttt catgggctgg
    13381 tggaagaccg cacgatggac ctaagcggtg gggtgctttc gggcatcctg accctggggg
    13441 ggaccatcct tggcatcagc cgcaatatcc cccaggctat gccgggcgcg aacggcagcg
    13501 cggacatgac ccaaaacgcg gtggacactt acaagcgcca caagcttgac gcgctggttt
    13561 gcataggcgg gaaggaaacg attaaagccg ggctgcacct gagcaaggcc ggcttgaata
    13621 tcgtcgtact gcccaaggca gcggataacg acattcccgg aacggataaa gccatcgggt
    13681 ttgataccgc ccgcgaaatc gcaacgcagg cgattgaccg cctgcattcc agcgcgaacg
    13741 ccacccaccg catcatgatt gtggaactcg cgggggtcag cgccggatgg ctgaccctgg
    13801 gcgccggcct ggcagccgga gcggacgtca ttctcatccc ggaatttcca tatgattttg
    13861 gtttgattag cgcggcgatt ggcgcgcgcc agaaagcggg cagaaacttc tcgctggtgg
    13921 ctgtggcgga aaacgcgcgg tcgcaggaac tggtggaatt tatggaacgg gcggcgcgca
    13981 agaaggcagg ccaagaggct gccagcgaca gcgcggtgag cgcggaaatg gagcgcatcg
    14041 gaaagagtta ctcgggcgaa acgatgctgc tggcgcgccg gctggaggac gaaaccggcc
    14101 tgcaaagccg cattaccatc ctgggcgcgc tggtgcgcgg cggcacgccg actgcttccg
    14161 accgtctgct ggcaacccaa ttggcggagt ggagcgtctt gatggtggag accgggcagt
    14221 tcggacacat ggcagtccgc atggacgacg cgatgagcag cacgccgctg gaggatgttg
    14281 tggacaagca caagctggtg cccgcagacc acggctggat ccgcggagcc tgtcaggtag
    14341 gcacctgcct gggggtgaaa aaccccggat tgtaactccc ttccggggta aacgaaaggc
    14401 agttcaaaaa gagcaggcat ttctattgga cagcccaaag tgggagaatg tgcctggttt
    14461 cgcctggttg tgattgcgca aatccgcgaa cgcccataga aacgggcacg gaggaggaaa
    14521 accaaaaagg ctgtccaact tttaggacag cctttttggt ttgtcgggct atttggagta
    14581 aaagtccttg cggatggcag gttcccgcgc cagcccggcc tccagcaggt ccatgaggtt
    14641 atccagcagg ctctgggcaa gcggatcttc gcggatgtca gctttgccgg agcgcagggt
    14701 ttcggcgctt tcgcccaggc gcacgacgag ttggtgcgta agcaggtggt aggctgcgcc
    14761 gagcaaatgc gcgccaagtt tggtatccac ccaggtagcg acgtcgtccg cgaggattcc
    14821 ctgggcgagg aactcgttga agaatgaggg gccgccaatc aacggcgcgt tttcatctac
    14881 gggcgcgctg ctgttcaggc tctccagaaa ggcgtgatgc tcgatggcgg aaagttcggg
    14941 ctcgcgcagt tcaaacagga tttccacctg gaagagccag cgcaaatacg tgaacatatc
    15001 ccggccgttg gaggaattga gtgtgttcgc cagcgctaac tttttttggc gggcgatgga
    15061 gagaatgtgc tgaaaaacat cctccatgcc ggagaaatac cgataatatg agcctttggc
    15121 gatgcccgag cctgccacca gggcgttcac cgaggcgccg cgcagcccgt tccgggcgaa
    15181 ctcgcggaca gccgcgtttt cgatttgagc gcgctttgtt tcggataggt tcaaaaaggt
    15241 ttttgtgggc ataaagcttt ccctgactgt gaccaaacgg tcatatttta tgtgcgggcg
    15301 cgggttttgt caatagaagc ttgaaaatgg ttggtttccc ggaaattggg ttagaatagg
    15361 cggatggatc acatttacag cccctggaga aaagtgtatt ttcaacgaga taaaaccgac
    15421 cccacctgcg tgttttgcgc ggcacaggag acagaggaca gcccggagaa cctgatcgtg
    15481 caccggggcg aacgcaccta cgtcattctc aatttatatc cgtacagcaa cgggcatttg
    15541 atggtggtgc cctaccagca ctgccaggaa ctggcggagc tgcccaagga aacgctggaa
    15601 gagatgattt gcaccgccag caaggctgtg gcgcttttgg agagcattta ccatccgcag
    15661 ggctttaatc tcgggatgaa cattggctcg ccagccgggg ccggcatcgc cggacacttg
    15721 cacatgcacg tcgttccgcg ctggagcggc gacgcgaact tcatctccgt catcgggcag
    15781 acgcgggtct tgccggaaac gctggaagag acctacgagc gtttgaaagc cgcctggaat
    15841 caggaggcat aataaaaagg agccgcgagg ctcctttttt gtgcctatga gtctttcttt
    15901 ttgcgggtga gacctgccag gatataggca agaccaacca gcacgatagc cagcggccaa
    15961 aacccggaaa attgaggcca gggcaggtac tttttgccgc ccaggagcaa gaacattacg
    16021 gcagacacaa cgatgagaat cagtcccggg cgccaggcat ctttttcatc ggggtcgaaa
    16081 acggatgcga gcatcaagcc cagcccgagg aaggccgcca ttgaagccca ggtgccgccg
    16141 tccaaagctc tttccgccca cagattggcg ccgacgcccg tgataagcgc ggcgctaatc
    16201 agccagccgc cttgctcgag aatcgcgctg cagaccactc ccagcagtcc caagggcacc
    16261 agcaggagcg gccaacttaa gaacggttcg agcgcgggaa cgtacggacg cgcgaaaagc
    16321 gccgccccaa tcagaaagag cgccgcgccg aaagcgattt gctgccgctt gcccatcatt
    16381 tctctcccag gctcttgata gtgcccaagc ctaccaatga ctgcgtcacc tgcgggctgc
    16441 cgtagtagga gacggagcca aacccattga gcgcaatgtc gagcgtatca gtggcccaga
    16501 ctttggcatc gcctgccccg ttgatgacca cctttgcatc ccggcttttc aaatcctggg
    16561 cggaatatgc gccggcgccg ttcaggagga gcgattgata gccgacctcg ccgctgatat
    16621 tgacattcgc gccgccgtca agctgtagct cgagcgtatc ggcgcgcacg ttttgcagcg
    16681 tgacatctac gccgccgttg agattgaggc gaaagctcgg caggtccagc gcgccgcagg
    16741 tgatttttgc gccgccaagc aggataagct gctccaaatt ggtaaacgtg acggtgtaga
    16801 tgatgggttc ggtggggagg taacgctctt tccacaacga ttcctcgtaa cccaggttga
    16861 gcgtggaacc gtccatctcc gcggtcagat gcggcaggat gttgtcttcc gcttcaatca
    16921 ccagcttgta ctcgctgccc tggaccaggc gcagttcgcc tccgccagta agcttgatgg
    16981 taaggaattc cgaaacagcg ggttcagagc tgacgacttt cccggagccc ttttcaacga
    17041 ccggggtttt tggcccggcc agatttcccg gtaaaccgca tgccaaagtg agcagtgcca
    17101 gcagggccac aaggaaggca atggtgcttt tcttgttcat aatcttcatc ctttctaaag
    17161 tattcattaa gtattgtaca cactttgccg tgcaaatagc aatatgccgg caagctccag
    17221 ggaaaatcag acgcgttgta aacggctcgg agagcctgcg tggaaaaagc ccagcccgaa
    17281 gtctggtcag gcaggtttta gcagcggaat gctcattcgg cgctcttttc catgctcttg
    17341 agctgccggc gcagccagga cgcagcctgg ctttcgtgct gacgaatgac cagcgtggaa
    17401 cagggcacgc gtgcggctac atcttcgacg atttcaccga agagctcgtc ttcctgcggg
    17461 atttcactcg aaccgataat tacaaggtcg gatttaccca gccgcgcgtg ccggataatc
    17521 gattccgccg ccgaatcaga ttgatcgagg ttcaggctcg catcggttgg gatgccattc
    17581 agctcgctca tcacgatttc ctgcaggtac gccatctggt cctcatattc ctccgggacg
    17641 atttcggagg gcaccacgcg cagaaagtcc acctgaccat tgttgattgc tgccagttcg
    17701 tgggcgagtt ggatggcaag tctgcagtga tacccgccgc ctacgggaac cagaatgttt
    17761 tgcagttgag ggagcccttt atcatacaga acgccgatgt tcacgatatt ctcacggaaa
    17821 agttcctgca cagtgttgtt aattagtttt tgaccggcgc tttcatgcgg catgcgcaga
    17881 agcacaagcc ggacgttgtt atccatttta atttcgttga taatcccctc cgagaggctg
    17941 ctggcagtta ctgtcttgga ataggtgggc acattgcgtt ccaccgcgta gtggatgaac
    18001 ttttccatga cgtattggcg cgtctggcgg aaatgttcta ccacttgctc ggttcgctcc
    18061 gcgctgcgat tcaataccag cagcatgcac atattcatgt cgcttttagc ttcagccagg
    18121 ctcgcgccga ttttcatgag cgtatccaga cctgaaaaat ccgtcacggg cagcagcaag
    18181 cggtatctgc cgggggagag gctgcgggtg tgcgcttcct gccaggaggc aaaaccgatg
    18241 cgcgcgtagt aatacaatgc gaagagcagc aggatgccgg cggtgaaagc cagggctctt
    18301 agctcggaga agcaaatcac aatcaggcag gtcagcgaag ccacaatcgg agtcagcggg
    18361 aagaacggga ctttgtaggg gcggtggatg tacggaaact tggagcgcag ttttatcatc
    18421 gccatattcg agaagaacag cacaaacaaa taacccgctg aggtgatgta actcaggaaa
    18481 ttgaccagac caattccggt gataaacatg gaaatcaggc cgattaccag gatggccatg
    18541 aagggtacgc gggctttatt cagcctggaa agcacagccg gccaggcgcc gtcgcggctg
    18601 agcgtgaagc tttcgcgcgt ggcggacagc atggacgagt tgaccgaggt aagcgcgcca
    18661 atcatgccgg ctgcgccgat gatggcgacg ccgagcatcg gcgagaactt gcggactgta
    18721 tccgagagcg cggtctcaga gccggccaac tgctgccagg gcgtcgtccc cagcgcgacc
    18781 gttaccgaaa gggaatacac cagcgtgatg ataatcaggc tgacgatgat ggcggtcggg
    18841 atgtttttgg tggggttctt cacttcttcc gcgtcatccg caatgacctc aaagccgacg
    18901 tacagcgcat agatgagcgc gatggtgcgc aggatggacg tgagattttg ccagaagtta
    18961 tcgccgatga agaacttgcc gccgttcagg aaggtttcgg cgttgaagcc gttaggcagc
    19021 aagaacccgc cgacaatgta aaacgtgaag gcgctcagca gcaggatacc catcacaatc
    19081 tggatgttgc caacgttggt cacgccaagg atgttcagga tgacgaacgc caggatggcc
    19141 gcgattgcca ccggaacgat gggcaagcca ggaatgaata ctgacaggga ataggcaaag
    19201 ccgaccgcgg acagggcgca ataaaaggtg ctggaaatgc tgtccatcga gccgaccagg
    19261 aaggcaagca gcccttttcc ccaggcctcg cgcacgtagg tcatagcgcc gcctgtttcg
    19321 gggtaggtgg tcgcgagttc gcagtagttc agcgcgatgc tgaagctcag gatgcccgcg
    19381 ataattacag ccagaattgt tgcaggcccc gccacggcgg cggcgtggcc agtgaggatg
    19441 aagatgccgg aacccagcgt tccggcagtg ccgagcataa tcacctgggt tagatttaac
    19501 tgccttttga gtgtgcgctt tgccatgctt ctcctcaatg acttttaaaa aggtattttc
    19561 ggcatcccgt gggaaagcaa taccaggatt gatttcccaa acgcggatta tacacccgcg
    19621 ggcgcttcct tgacgcctgg ttttcatctt gaacgttggc ctcttccgga aaagtgtatt
    19681 tggaacaatc gatattgtat aatttaagct gagcggaggc agaaatggat ccaaaaaatc
    19741 cccttgaaaa tcacatcgtc atggcagacc aggtctggca cgccagaggc gtggaagatg
    19801 cccttgccgg tttggaaacg aacgcggagc aggggctgac gctggctgag gtgcaggaaa
    19861 ggcaaatgcg ctgcggcttt aacgagcttc aggagaaagc gctgccttcg ttttgggagc
    19921 tgctcgggcg ccaactgaag gattttattg tcatcctgct gatggtggct gctcttgttt
    19981 ccgcgctgct gggagattgg attgaggcgg gcatcatctt gctcatcgtg gtgctaaacg
    20041 caatcctcgg ggtggtgcag gaaagcaagg ctgaggcatc gctggcggca ttaaagaaaa
    20101 tggctgctcc ggaatgccag gtgctgcgcg agggaaggcg cctgaccgtg cccgcgcggg
    20161 agctggtgcc gggcgatatc gtgttcgtcg aggcgggcaa ttacgtgccg gctgatatcc
    20221 ggctgctgga gtccgtcaac ctgcgcgtgg aagaagccgc gctcacaggg gagtccgtac
    20281 cggtacagaa aaacgcctcc gctgtgctgc cggataatgc cgccctgggg gaccgcaaga
    20341 atatgctttt ttctgccacg ctggtgaatt acggccgcgg gcacggcgtg gtgaccgcca
    20401 ccgggatgaa cacccagatt ggcaggattg ccggaatgct ccaatctctg gatgaaggga
    20461 aaacgccgct gcaggaaaga ctggataaac tgggcaagac tctgggcctg ttcgcgctgt
    20521 taatttgcgg gctggtgttc gtgttggggc tggcgcgcag cggcgtgttc gcgcagggct
    20581 tcacgcgggc aagctccggc gcaattgtgg aaagcttcat ggttgccgtc gggctggcga
    20641 ttgccgcggt gccggagggt ctttcggcag tcgtgaccat cagcctggcg ttggggatgc
    20701 gcgagatgat caagcgccac gcgctgatcc gtaagctgca ggcggtcgag acgcttggtt
    20761 cggtcagcgt gatttgttcg gacaagaccg gcacgctgac cgaaaaccgg atgactgtga
    20821 ccgtagtgga catggcgaat cacgttttgg attttacgca cgaagccgag aacggcgtaa
    20881 tccgagggct gcaggaagtc ctgcgggtgg aagcgccgga tgaagaccag gtcaggatgc
    20941 tgaaagagca cccgaccctg ctgcacatcc tgaccggcgg cacgctctgc agtgacgcgg
    21001 tgattgagcc gcaccctgaa aaagaagacc atattgtgat gattggcgac ccgaccgagg
    21061 gcgcgctggt ggtcgccgcg gcgcgcatgg ggctttccaa aaaactgctc agcgaagcgc
    21121 tgccgcgcga ggctgagctg cctttcgatt ctgaacgtaa gcgcatgacc actgcccacc
    21181 gcctgcctgc cagccgggaa gagctcccgg aggcgctgcg ggacctgtgg aactgggaag
    21241 cgtcggaaga ggcgcgctac ctggcctata ccaaaggctc ggtggatggt ttgctaaaca
    21301 tcgcggattc cttatttgtg gacggtcagc tggtgccatt ggaagaagaa tggcgcgctc
    21361 gcattgaagc ccgcaacgcg aacctggctt cgcagggcgt gcgcgttttg gggctggcat
    21421 accggctctt gccggaactg cccgaaaatt tggaagccgc gcatgtcgag aaggacatga
    21481 cctttgtggg catggtgggg atgattgacc cagccagacc ggaagtgaaa gacgcggtgg
    21541 ctctgtgcaa atccgcgggc atccgcccga ttatgattac cggagaccac ccgcttaccg
    21601 ccaggcatat cgcggaagac cttggtatcg ccagcaacgg caaggcgctg actggcgccg
    21661 atgtggaaaa actatccgac gccgagctgg aaaacgcggt gcgagaggtg tccatcttcg
    21721 cgcgggtttc gccggagcac aagctgcgca tcgtgcaggc tttgcagacc aacggcgaag
    21781 ttgttgccat gaccggagac ggggtgaacg acgcgccggc gctgaagaaa gctgatattg
    21841 gcgtggcaat gggcatcacc ggcacggacg tggcgaaagg caccgcggat atggtgctga
    21901 cggacgacaa ttacgccagt atcgtggcgg cggtggagca ggggcgcgtg atttatgcca
    21961 atatccgcaa gtttgtgttc ttcctgctct cctccaacct ggcagaaatc atgattattt
    22021 tccttgccac gctcctgggg ctgccgatgc cgttgaccgt aatccaactg ctgctgctga
    22081 acctgctgac cgacggcttg ccggctttgg ctctggcggt ggagaagggc gatccagacg
    22141 taatgcagca gcccccgcgc cctaaaaacg agcctgtcat caacaaaagt atgacaaccg
    22201 gcctggtcat ccaaaccatc ttccagactg ccgcggtgct gggcgctttc ctgttggggt
    22261 tgtggcggca ccccggagcg gctctgcctg cgggcgcgaa cccattcctg acactgctgc
    22321 gcttcgactg ggccggcgtg gacgttttga cggctgaaac catggcgttc gtgacgcttt
    22381 cgctgtgcga gctttttcgg gcgtacaccg tccgctccga aagggtgtcg gttttcaaat
    22441 tgggcgtgtt ctcaaacccg gccatgcagc cggctgtgct ggtgagcctg ctggtgatgc
    22501 tgctggtggt aaatgtgcct ttcctgcagc cggtattcaa cacgcatttt ctggcggcac
    22561 aggaatggat ggcggtcatt ggtttggggt tggtcccggc agtggttgaa gaaatcacca
    22621 aactgtttct gcggaaggca gacgggaaaa agaaaaatca gggtcaggct gaatgagcag
    22681 cgcgctgggc tgccggccag gcattgcctg gacttccatt cccgatttcg tggaaaaggc
    22741 tgaggcccag tccgcagcgg aaaagggagg ttgtcgggcg gtttgcggct gggaggggaa
    22801 agccgccggg cagggagatt aagcagaagg agattttcaa cggaatgaac agcacgcaac
    22861 gattatgggc attgggccag tcagtctggt atgacaatat tcagcgcagc ctgctgcgga
    22921 acggggagat ggcggggatg attgcgcgcg aggaaatccg cggtgtgacc tccaaccctt
    22981 caatcttcat gaacgcgatc gccaaatcca gcgattacaa tgagagcctg aagccgcttg
    23041 gcgatccagc cttgagcgct gaggaagtct ttttccggct ggcgattgag gatattcagg
    23101 aggcggcgga tttgttcctg ccgctgtatc agagctcggg cggcgcagac ggctacgtca
    23161 gcctggaggt aagcccattc ctggcggata agagcgcgga gacgctggcg caggcggagg
    23221 cgctttgggg gcgggtaaag cgcccgaacc tgatgattaa gattcccgca acgaaggctg
    23281 gtttgccggc gattcgggcg gcgacagccg caggcattcc ggtgaacgtg accctgattt
    23341 tctcgcgtga gcgctacggc gaagtgatgg aggcttatct gagcgggctt gagcagcgcg
    23401 cgcaggccgg actgccgctg gaggggactg cctcggtggc gtcgttcttc atttcacgcg
    23461 tggattcgca cattgacccg cgcctgcaca ctatcggcag cgcggaggcg ttggcgctgg
    23521 ctggcaaggc cgcgattgcc aacgcgcgcc tcgcgtacgc gcagttttta caggtctttg
    23581 gctccgggcg ctttgcgagg ctggaagctt tgggcgcgcg caagcagcgg ccgctgtggg
    23641 catcgaccag caccaagaac ccggcttacc gggatgtgct ctacgtggag aaactgattg
    23701 gcgcggatac ggtcaatacc atgccgccgc agacgctggc ggcttttctg gaccacggcc
    23761 aggtgcgcga gagcatcacg gaagacctgg aagaagcccg gattctgttc gaccgcctgg
    23821 aggcgcttgg aatttcgatg ccgcaagtga ccgcgcagtt ggaaatggag ggcgtgcaag
    23881 ccttcgccga cgcgttcagc gttttgctta acgtcctgga aaagcgccgg aaggcttgag
    23941 gagcatcaga ttagaatcat cagcggcgcc cgcgggcgcc gctgttttgc tgttcctgcc
    24001 gtcctgcttt tgcggcctta cttcgcgacc aggccctgat acaggcgcag gtatttgagc
    24061 gcggacctgg accaggagaa gtcctggcgc atgccgcgtc gctgcatcgc ctgccaggcg
    24121 ccgcggtccg ggaagagatc gagcgctctt gtgagcgcgc cggcaaagtc cgcggcggtg
    24181 gcgggcttga acaagaagcc agtgccgctc tcagggtgga tgctgacgtc gcgaatagta
    24241 tcctgcaagc cgccggtagc gcgcgccagc gggacgcagc cgtagcgcat ggccatcatt
    24301 tgcgctaatc cgcagggttc gtagcgcgat ggcatcagca ggatgtcgcc gccggcgtac
    24361 atgcggcgcg aaaggttgac atcgaagcgg atggctgcgc gtacgcgcct ggggtgcgcc
    24421 ttttccagtt tgcggcaggc ttcttccagc tccgggttgc ctgtcccgag cagaatggcc
    24481 tgccagcggc gctttgccag gctggccagt ccctggacgg ccagatccac gcccttttgc
    24541 tggtccatgc gcgagacgag caccagcagg gggaggttgg ggtcggggtc caggtcaaat
    24601 tcctgcgcga gcgcggtctt gttggcagca cgggcatcga gcgtttcggc gctgaaagag
    24661 gcgggcaggc gttggtctgc gctgggatcc caggcgtcca tatccagccc attcacgatg
    24721 ccggagacga ctttgtgccg ggtctggagc agtttttcca gtccgcagcc gaattcgggg
    24781 gtgaggattt cctgcgcgta ggtttcagaa acggtcgtca cgcggtcggc ggtctgtatt
    24841 gccagaggca gcgggaattt tttgccccac cagggcaccc ggctgtagcg gctgggcggg
    24901 atgccgtagt tgtcgaaggc ggcttcggtg cccgcgccca tgaagggcag gttgtgcatg
    24961 gtgaacacgg ttttagggtg cgcggcggcg ggggaggctt tacggcgcag cttgagcagg
    25021 tacaccacca gggcggtgtg ccagtcgttg gcgtgcacga tatccggttt ccactccagg
    25081 cggtctataa accccagcgc ggcggcggag aagaacacgt atttgcgccc gtccgcggcg
    25141 gaatccgggc tgtagaccgg gctgttggat atgggttcgc cgccaatcag gtagaccggc
    25201 acgccattca cctcgctgta gtagacttcc gcgttgagcg cgccggtttt cgattcgacc
    25261 ggaaagctga acaaaaattc ggcagtgggc agcttttggc gcacagcgtc gtggtagggc
    25321 agcagcaggc gcacgtccgc gcggacgcca ttggtatctt cggcaggcag agcccgcagc
    25381 gcctggggaa gagaaccggc aacatcgccc aaaccgccta ccttgaccaa tgggtcggcc
    25441 tcagcggcaa ggaacaggac cttcagggtt ttcatgcagt tctccttatt cacggcggga
    25501 ttttatggcg ttaatcagtc cattggtgga cgaatcgtgc gtattcacga gcgcggcgcc
    25561 ttccaattca ggcaggatgg ctttggcaag ctgtttgccc agttccaccc ccatctggtc
    25621 aaaggaattt atccgccaga tgaccccctg cgtgaaaatc ttatgttcgt agagcgcgat
    25681 gagggtgccg agcgtttcgg gggtgagttt ttggtagagg aaggagttgg tcggacggtt
    25741 gcccgggaag gttttggcgc gcaccagcag ttccagctcc gctccggcga agccttgggc
    25801 agccagctct gcccgcgctt cttccggcgt gcggcctttc atcagggctt cggtctgcgc
    25861 cagaaagttg gagagcagga tggcatggtg gctgccgagc gggttttggg tttccacgcc
    25921 cgccaggaaa tcacaaggga taagcttggt gccctgatgg ataagctgga agaaggcgtg
    25981 ctgcccgttg gtgcccggct gcccccagat gatgggaccg gtggagtagc gcagaaactc
    26041 accggtgcgg ctgacgcctt tgccgttgct ttccatgtcg ccttgctgga agtatgccgg
    26101 gaagtattcc aggtactgtt catagggcag gatggcgtgg gtctgcgcgc caaagaagtt
    26161 gttgtaccag atgcccagca gcgccatgag cacgggaatg ttttgcgaga aaggagcgct
    26221 gcggaagtgc tggtcagccg cgtaagcgcc gcgcaggagg gcttcgaagt tgtccatccc
    26281 gacgaaaagc gcaatgggca gcccgatggc ggaccaaagc gagtagcgcc cgcccaccca
    26341 gtcccagaat tcgaacatgt tcgcggggtc gattccgaat gcccggacgg cctgggtgtt
    26401 ggtggacatg gcggcgaagt gctgcgcgat atagcgctct tcgccggagc gcgccaggaa
    26461 ccagtcccgc gcggtatggg cgtttgtcat cgtctcctgg gtggtgaagg tttttgaggc
    26521 aatcaggaag agcgcggtct cggggttcag cagtttcagc gtttcggcga tatcggtgcc
    26581 gtccacattg gagacaaaat gcacgcgcag accgtgtttg ccgtagggtt tcagcgccgc
    26641 gcagaccatc ttgggtccca ggtccgaacc gccgatgccg atattgacga tatccgtaat
    26701 tggtttgccg gtaaagccgc gccattcccc ggagcgaacg ctttcgctga agcgg
//
LOCUS       DAOQAD010000016        25934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_1871_length_25934_cov_5.746126, whole genome shotgun sequence.
ACCESSION   DAOQAD010000016 DAOQAD010000000
VERSION     DAOQAD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 25934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25934
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1871_length_25934_cov_5.746126"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKL60_02565"
     CDS             <1..336
                     /locus_tag="PKL60_02565"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW95051.1"
                     /translation="EAGLNPFLMEMVNIREQCTWAHGHDREGALAKAKDLTASAISKV
                     SFLQPLDMISVPVSRKAMVIGGGVAGISAALDLAEQNIPVVLVEKEPTIGGVMAQLNK
                     TFPTMDCSI"
     gene            352..2241
                     /locus_tag="PKL60_02570"
     CDS             352..2241
                     /locus_tag="PKL60_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNW95052.1"
                     /translation="MVDAARQPLIEVKTFTEVEKVEGFVGNFKVTLREKAKYVRADRC
                     NGCGACAKVCPIEVPNYFEMNLAPRKAAYIPMSQSVPLIYNIDMDACIHCYKCVEACG
                     PLEAIDFSMQDKIVEEEIGAVIVATGFSHFDPSPMEEYGYNRYPNVITSMEMERLNNS
                     AGPTAGSLIRPSDGKKPQRLAFINCVGSRDKRFVESCSNFCCMYSIKNAVLVKQMNPN
                     ADVTIYYMDIRTPSKGYEEFYNRARSMGIHFIQGRPAMITEDPESHNLFIHTEDIALG
                     KVVEHEYDLVMLNQAAVPQPDQNHMSSVLNVAQSPGGWYMEYHPKLRPMDSPTDGIFF
                     AGACQGPKDIPASVAQGSAAASRASRVLHSKTWQIEPTIANVWSDRCVSAQGKACGIC
                     ERSCPYGAIDYTQGKAAEVITAKCHGCGACAGECPHDAITQLHYTDAQILSQIRTLLA
                     DKPEKKILALRCHWCSYGGADLAGTSHFEYTSNERGIKVMCSARMDADFIYEAYRLGA
                     GAVLYSGCHPQDCHYITGQVIGAKRAKRLETIFERMNMSPGRFRVEWISAAEGDKYAR
                     VLNEMQAALDEIPPEALHAEIERMRPDMAKRLTRLPNIPGVQRAMDHTEVVVNTILSR
                     KGEPV"
     gene            2298..2819
                     /locus_tag="PKL60_02575"
     CDS             2298..2819
                     /locus_tag="PKL60_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNW95053.1"
                     /translation="MCYSCGTCTSKCMIQQKLEPEYNPRRLLRMVMMEMRTESYANPT
                     TWLCSSCDLCYPACPQQIHISGVITAVKKIAVQAAQKTPLPTAVVDQQTCVACGLCAE
                     VCPYDAITIETRKLPYRGSIPVAVVNAGLCMACGACGAACRSNSIGIPADTSDEEVVE
                     NIWSWLNPEGALN"
     gene            2819..3259
                     /locus_tag="PKL60_02580"
     CDS             2819..3259
                     /locus_tag="PKL60_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNW95054.1"
                     /translation="MDNRPKIAFFQCQWCLYAPEDQEWVDKSLPENIHLIKVPCTGRI
                     DPLFVLNAVQGGADGVVISGCLPEKCHFKEGNLAARRQLDAFSSFLDYIGYDEARVRF
                     IWLDLQDRGRIQRELTEFESLLKKLNPAKSLATRVPETEGGAND"
     gene            3252..4220
                     /locus_tag="PKL60_02585"
     CDS             3252..4220
                     /locus_tag="PKL60_02585"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNW95055.1"
                     /translation="MTELEQKIRAEAARLLSEGLVDVVMAFRKEDAPLRPQPAFFTCV
                     EDLENLIYNGLCANNLANYLTRYPDETRIGMLVRGCENRSVNALVVEKQHSRQNLYLI
                     GVPCTGIIDWRKVVALTGEDILGYEETDSSLHVRTKRQSYQLPRRELLHASCQRCVHP
                     NPLSADIVLGEALPEGDPALIRERVEAFEALTRAERTAYFKQEAERCIRCYACREACP
                     MCYCEECFVDHNSPRWTESGTTPAGTHAWHIIRAFHQTGRCTSCGACERACPMDIQMT
                     YLTDKLGKDMWDSYQFAAGMDAKSQPPFASFTLDDRKHFQAGGMHS"
     gene            4217..5242
                     /locus_tag="PKL60_02590"
     CDS             4217..5242
                     /locus_tag="PKL60_02590"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNW95056.1"
                     /translation="MKYILPKQDLNALLERWSAEYAVLAPQKSGAYAEFLPYTPRSEL
                     CLEEPHNTRYPPKALFFPQSEAILQYKRTTGQLESISAVAQKRVIFGIRPCDAKAVSL
                     LDTVFAEGDNQDPFWQARRAETLLIGLGCHAPCATCFCTSAESGPFHTDGLDALLTDL
                     GEIYLVELLSERAGGLFDALAPASSILQENAQAVQSAAECALPKAFELDGLKGKLDRV
                     FDQDIWAQLAQSCLGCGICTFLCPTCFCFDLVDEAQRDTRVRNWDTCMFRVYSLEASA
                     HNPRPTRKERTRQRLMHKYSYWWEHADKLGCTGCGRCVRYCPVGLDIRAMIRTAGAYE
                     NEVLHAN"
     gene            5232..6095
                     /locus_tag="PKL60_02595"
     CDS             5232..6095
                     /locus_tag="PKL60_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNW95057.1"
                     /translation="MPINTSTRNPYQPTPMRVLRAYYESTDRSLKTLELSFENHADRQ
                     AFFRKFNPGQFCQLSVFGKGESPLGVASAAWEGDFVRFTIQRMGSVTKALHELKPGDT
                     LGMRGPLGNGFPLSEWKGKNLVIIGGGCAFSTLYALTKHLQYPQNRPDYAQLVVIYGA
                     RSSGLCMYKQDIQSWYQREDMEVHQAIDVPEESWDHHVGYVPDVVRQIAPSPDNAVAI
                     VCGPPIMTKFTLPALVSLGFPAEAIFTSLEKRMKCGLGKCGRCNIGSKYICKDGPVFS
                     LAELNALPADI"
     gene            6106..7098
                     /locus_tag="PKL60_02600"
     CDS             6106..7098
                     /locus_tag="PKL60_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNW95058.1"
                     /translation="MSQRGIPQKSKNEHSMTIRRGMVTRNYLMTWDLDKCVGCQIGPI
                     VCPKQALTHVPVQLENGRIAVKASVDVDEKKCVNCGMCVVSCPTHAISLTINGKTEIP
                     VLEYETYPEYLSKTIFRRELFDFSLKDFVIDNCSANVISYDETRDTMRVDFENCIHCR
                     QCEIASKGAFEVRQAWQGSVELHRERCVEGCFACADICPTRALHIDDQGELVLADYYC
                     IKCGACMQVCPIKPKYESQEFEFEYQGMQVKRSRDVLLNADELAVKVERWRLNHKPVS
                     SAAWTEALRKLADDNAKTVEIDRKRALRRADLVRALRGHTLTETRPVAKQSKQS"
     gene            7191..7469
                     /locus_tag="PKL60_02605"
     CDS             7191..7469
                     /locus_tag="PKL60_02605"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNW95059.1"
                     /translation="MSNRLVLLGKQLISDTSSAEIRRIEDLLKQENIPYRLQTQRSRG
                     SVGSFMDSRSYAASNIALYKGAATPTFVYAIYVRPKDYKRAQSLMQVR"
     gene            complement(7510..8865)
                     /locus_tag="PKL60_02610"
     CDS             complement(7510..8865)
                     /locus_tag="PKL60_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNW95060.1"
                     /translation="MLIVMSDLHLAESKSLGLGSTQFNHNLPAGVYQEYFREIAESLD
                     DGVVGEVDLVLAGDIFELTRSASWLEDSLRPYGHLDDIAEGSAREAKILAILKEIAED
                     ARVAETLQSIRSLENSFQRPTRVHFIPGNHDRLANATPAVRSAVRRMLGIADSPAPFD
                     NQYLHPFDGQPGVLVRHGHEYDPNNFGINLSAEASLPLFMPAEWYKRPVLGDITTLEL
                     AARLPKVFKDYYTAEGILKKTPLLLLYQRIMEFDDVRPANALMSFLFSTPGLNRDETW
                     GYLEPVFKWIRDEIARDEALKQQFLTLGALVGLPLFTLKILLDKRIWKRRIPFWVFEK
                     LIESLSGKYTLGSLMGMIQKEECLSRSDSTVTCMVSGHTHTPAVELVRTSGGRERYYL
                     NSGTFRNVISAAPDSSSFGRLRSKARVLIYGPGEKNPEYARETGWSFDYSSRFGYGSE
                     R"
     gene            complement(8946..11024)
                     /locus_tag="PKL60_02615"
     CDS             complement(8946..11024)
                     /locus_tag="PKL60_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95061.1"
                     /translation="MIFLFFCVVLAAGAPLAPASAGSNSQSAEGGVVIYLPILFRAPD
                     PPSIQSMSIGPDGGTFTAVAVDPGNPSILYLGTYGAGVYRSTDQGVSWIQRINGLGNT
                     FIQSLAVDPQNGNVLYAGTYTGGVYKSTDAGANWFASNGSLTDPIVVYDLEIDPQNPS
                     MVYFAGRIKNTLIGVVYQSWNGGLDWNLLLRGDQFNTDDYFYDIDVDPTNSSVLYLAA
                     HEHGFYKSVNGGASFSAINSGVSDLSARSMALDSGNPSLIFGGVWHGEGVYRSTSGGT
                     SWSRVSSGLPTDVEVYRLYLDQSVSGTKPVFACTYENGLYRSSNNGSSWGSAGLSGQF
                     LYDFTIAPGSPQNWYAGLSYRGLYRSTNQGADWAPSHSEVSSHSINGFASISAQPGYV
                     YAGVYGIGVVRTNDGGTTWQEVNSGLGSRLVTWLTAANNKLYALTPSAVYVSSGGSWT
                     TINNPQSAGYDPSWYLETVLAGISQPEDDAAQEILTALEVQTEAALTSTPLISLTAIG
                     STLFGGSAGAGLWKYSGGSWTQVGLNGYSIPSLSVDTALGRVLLTACDSSGICTARYL
                     KDGALTSINQGLEGINTNQILARGDDYFAATVNGIYMRDNTGSLWIPVGAAGNNILSI
                     SFHAQNTALMAAGGAGSTFWSGDYGAHWQLATPVLTRWSYQSVILDPADSSRVLFGTR
                     EAGAFRWSGE"
     gene            complement(11136..12185)
                     /gene="ltaE"
                     /locus_tag="PKL60_02620"
     CDS             complement(11136..12185)
                     /gene="ltaE"
                     /locus_tag="PKL60_02620"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HNW95062.1"
                     /translation="MSQYDFRSDTVTRPTLRMRQAMASAEVGDDVYGEDPTVNRLEAR
                     AAELLGKEAALFVTSGTMGNLLAVLAGCERGAEVIMGRQGHTFLHEAGGVSVLGGVSM
                     HTIPNQSDGTLALEDLHGALRDPADFHEPLSRMVILENTQNACGGIPLSAEYTDSVAA
                     FARLHGLVLHIDGARIFNAAVALGVPAAELVRSADSITFCLSKGLCAPVGSVLCSSRE
                     TISKARRLRKMLGGGMRQAGILAAAGLVSLDHMIERLAEDHQRARELADALRAVPGLR
                     IDKGTPYTNMVYLALEPEVRQDAAEIVSLLAEQGVLIGATGARTFRLVTHHDVDDEAL
                     KACIRGFSRVINRQN"
     gene            complement(12182..13114)
                     /locus_tag="PKL60_02625"
     CDS             complement(12182..13114)
                     /locus_tag="PKL60_02625"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNW95063.1"
                     /translation="MNAQTKELRFEQSEPERLDKFLAEVLPEHSRSQFQELIASGKVW
                     VDGQVCLKPALKLSAGQMVSVELLAPQAQGIPAENLPLDVIYIDENTIVINKPAGLIV
                     HPGAGHSTGTVVNAALYRWPEIRQVGEPERPGVVHRLDKETSGVLVLARTQSAYAWLV
                     RQFKSRKTEKYYLALVDGHPPTPNGRIEAAVGRDAKFRQKMAVVYEGKGRKAVSEYFT
                     KQSFRDHTLLEVRPFTGRTHQIRVHLAYLGCPVVGDRVYGRRKASFPLERFFLHAAKL
                     RIILPGEKDLTEFQAALPDDLKEVLNTLSAMEDV"
     gene            complement(13128..13649)
                     /gene="lspA"
                     /locus_tag="PKL60_02630"
     CDS             complement(13128..13649)
                     /gene="lspA"
                     /locus_tag="PKL60_02630"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNW95064.1"
                     /translation="MKTKLKQNLLLLGIALVVILVDQATKFIIRQNLALGQAWLPFAP
                     SEFFRFVYWQNTGAAFGIFQDGNMVLKVLTSVIILFILFYYQRIPYEQKFVRICLAIM
                     LGGALGNLIDRFNFGYVLDFIAVGSFPVFNVADSCVTVGVGLLILALLLEDKKARTHS
                     LPDAENSREESKG"
     gene            complement(13664..14254)
                     /locus_tag="PKL60_02635"
     CDS             complement(13664..14254)
                     /locus_tag="PKL60_02635"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNW95065.1"
                     /translation="MDELNVKSVRLLSGAGEVVDYRINPLPRQLGQKYKNLFPQIRNA
                     ILELPVQESAQKLLDGLNLVVQVEGKYYEIQPDEVEVHTSAREGLEVASEGPYLAALD
                     IHLTQALVYEGLAREFIRRVQTLRKDGGLDIADRIRLHYSATPQLAEAVRSSADLIKA
                     ETLAVELAEVEDTGSWITASDSFDNEQLTLRIQKTR"
     gene            complement(14300..16705)
                     /gene="ileS"
                     /locus_tag="PKL60_02640"
                     /pseudo
     CDS             complement(14300..16705)
                     /gene="ileS"
                     /locus_tag="PKL60_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
     gene            complement(17327..17398)
                     /locus_tag="PKL60_02645"
     tRNA            complement(17327..17398)
                     /locus_tag="PKL60_02645"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17364..17366),aa:His,seq:gtg)
     gene            complement(17433..18701)
                     /gene="hisS"
                     /locus_tag="PKL60_02650"
     CDS             complement(17433..18701)
                     /gene="hisS"
                     /locus_tag="PKL60_02650"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNW95066.1"
                     /translation="MEKIVRSVKGTRDFYPEEMAVRRWLISRIQQVSAAFGYQEYDGP
                     CLETIDLYAAKSGEELVKEQAFVFPDRSGENIALRPELTPTLARMIASKQNELTFPCR
                     WWSFGPFWRYERPQKGRTREFFQWNIDLIGSDEVSADAELIAVAAEFLRSVGVRPDQV
                     KILINDRRLMDAHLARIGIPLELRPAVLRQIDRIDKQPADVWDKNALDLGLTSGQLRE
                     LKLLLGSADLWQQSPELTALFTELEGLGVREYAAYEPKIIRGLDYYTGTVFEARDTAG
                     NFRAILGGGHYANLVGDVGGRPLPGVGFAMGDVVILLLLESLGLLPEISAAGEAVFVS
                     VFNENCRGASNRVSTRLRRAGFKVFSYPGADKLTRQLKYADKLGARAALVIGPDEIDR
                     SEVVLKDLANHSQQVVPEAELEKRLAEILA"
     gene            18793..19167
                     /locus_tag="PKL60_02655"
     CDS             18793..19167
                     /locus_tag="PKL60_02655"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNW95067.1"
                     /translation="MKISEILEISQGSLLTPYANPDIEILGGFAGDLMSDVLASIQPE
                     SVLITGLSNPQVIRTALIADVRVVIFARGKQPAQETIKLAIEEKLPVISSDLGLYEIS
                     GRLLQHGLPSFEKRMLHAFDSD"
     gene            19176..20135
                     /locus_tag="PKL60_02660"
     CDS             19176..20135
                     /locus_tag="PKL60_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNW95068.1"
                     /translation="MFSDKPAVTDDEAAKITRVEELAYELSVGEVMTSKVFSLRPNQT
                     MREALDEFKQHRISGAPVEVDGKLLGILSIEDMIRSLRDGRIDLTVADYMTADVITAR
                     KEDPVIEALKLFVKSRVGRLPVLDSDDKLVGILTKSDITNGLLKALQNDYEAEELIRY
                     RASHLFEDIVSDRSTLTLRYNVKRNDFSHGGAASNNIKKALLRLGATPQIARRCGIAV
                     YEAEMNLIIHTNHGGVLRVEIEPDRITMEAYDDGPGIPDISLAMQPGYSTAPQEIREK
                     GFGAGMGLVNIKSCVDEMNLASSLDRGTNLYMVMHLDKTRHFS"
     gene            20149..20499
                     /locus_tag="PKL60_02665"
     CDS             20149..20499
                     /locus_tag="PKL60_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95069.1"
                     /translation="MRLNEIQNQLQLKTLTAPTISDNVEIRTGYTSDMLSCVMTGALS
                     GSVWVTLMAHNNIVAVASLLDIPAIIITENAQPDELTIARAREREIALFSTARPTFEI
                     VGKLWELGIRAEES"
     gene            20510..21226
                     /locus_tag="PKL60_02670"
     CDS             20510..21226
                     /locus_tag="PKL60_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNW95070.1"
                     /translation="MIEFRAELHVHTVLSACAGTDMLPGPIVEAALFNEINLIAITDH
                     NAGYNAAPVIKAGRRQGLMVLPGMELETEEEVHSLCLFDTLDQLDALQKIVDARLPFY
                     PNNEDFYGPQWVVDEKGNKLRPETRQLLFPVQISIQRACRIVKDLGGLFIPAHINREA
                     NGLLSHLGTVPPDLDVQILEINRHTTKEACLKKHPELARYRLIQSGDVHYLDGFLGAS
                     TFRAKNSSLKAITEGLLSSL"
     gene            21840..22358
                     /locus_tag="PKL60_02675"
     CDS             21840..22358
                     /locus_tag="PKL60_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNW95071.1"
                     /translation="MTPSENNVSANPLENPEQKAIIDAIIDENLHLNGATMVILNQVQ
                     EKIGYISKPVQAYIADRLGVPVSKVHGVVSFYSFFTTSPRGKHTIKFCLGTACYVGGI
                     DQIIEKAKQLLNIEPGNTTPDGMITLEVCRCVGACSQAPVVVIDDNIRGRMQPNRFPK
                     LIQDIQTQETAH"
     gene            22355..22900
                     /locus_tag="PKL60_02680"
     CDS             22355..22900
                     /locus_tag="PKL60_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW95072.1"
                     /translation="MREIALHILDIAANSISAGARNIAISIVGNHREDSLEITITDDG
                     KGMSPEMAAMVTDPFTTSRKERNVGLGIPLLKAAAEACNGSFRLASEPDKGTKVQVSF
                     QLGHIDRMPLGDLPGTFLGLLIGTPEVHWTFRVKNDEKEFTFDDAELKKELGEDCLAE
                     PAVIRYVRELFTEGIEALALN"
     gene            22911..23279
                     /locus_tag="PKL60_02685"
     CDS             22911..23279
                     /locus_tag="PKL60_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNW95073.1"
                     /translation="MPTIKSLEDLKKMREEALRKQELKTTTGKKEIVVGMGTVGIAAG
                     ARETLKAILEFVDSNHLENIIIRQTGNIGIDSFEPVVQVILPGEEKVTYGHVSPDVVK
                     IIMEEHVIKGKIVADYQVKS"
     gene            23331..25115
                     /locus_tag="PKL60_02690"
     CDS             23331..25115
                     /locus_tag="PKL60_02690"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNW95074.1"
                     /translation="MAFYRSTVLVSVDPVCVEKGAYELIDALNDELIKQGLIDEVRIL
                     ETPRLGDPQKFGPDMVVYPEGTHYTNLNYYEIPRLVEEHFIKGRILKQYAAKMQDAID
                     EELSAPKSKEIRVVLRNVGVIDPRNIEDYIANDGYQALGKVLTEMSPEEVIQVVLDAK
                     LRGRGGAGFPTGLKWRFARQAAGDIKYIVCNADEGDPGAFMNRRVLEGDPHSVVEGMI
                     IAAYAIGARQGYIYCRAEYPIAVSTMNLAMKQARALGLLGKNILGTDFSFDLEMRMGA
                     GAFVCGEETALMASIEGGRGEPRVRPPFPAQKGLWGKPTNINNVETYANVPQIILRGA
                     EWYASMGTEKSKGTKTFALAGDVKNTGLIEVPFGIKLSEIIYDVGNGIKGDKKLKAVQ
                     TGGPMGGCIPASMIDLPVDYETLGQAGSIMGSGGMIVMDEDTCMVDIARFFMEFIQEE
                     SCGKCVPCRVGTRRILEILEGICEGRGKEGDIDTLRSLCAEIKENSLCGLGQGAPAPV
                     VSTLNHFLDEYEAHIKEKRCPAKVCRALIKYQIMDGACTGCTVCARNCPVSAITGERK
                     QTHVINQDICIKCGICHQVCAFDAITVI"
     gene            25173..25628
                     /gene="nuoE"
                     /locus_tag="PKL60_02695"
     CDS             25173..25628
                     /gene="nuoE"
                     /locus_tag="PKL60_02695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNW95075.1"
                     /translation="MKTTSVVDEAIQQHGVSRSELIPILKTIMMRLGFISPEVVLQLS
                     QKLNLPSIEITSVATFYRMLSTAPRGKHVIQFCESAPCHVVGGNQVWQAITKTLNLQA
                     GETSADHRWTLITTSCLGVCGIGPVMLVDTDLYGNLTPEEVPHILSRYD"
     gene            25638..>25934
                     /locus_tag="PKL60_02700"
     CDS             25638..>25934
                     /locus_tag="PKL60_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNW95076.1"
                     /translation="MKTSRSMVLISHDPVSIQQGADAVLEAFQQELNRLQLQDEVSLS
                     LVEDIGRNYASPLVIVYPEAVIYGRVSVEDVPHLVEEHLFKGRIVEEKIAPAHEL"
BASE COUNT         5916 a         7535 c         7090 g         5393 t
ORIGIN      
        1 gaagccggct tgaacccctt cctgatggaa atggtcaata tccgtgagca gtgcacctgg
       61 gcgcatgggc acgaccgcga gggcgccctg gcgaaggcga aagacctgac cgcttccgcc
      121 atttccaagg tatcgttcct gcagccgctg gatatgatca gcgtgccggt ctccaggaaa
      181 gccatggtca tcggcggcgg cgtggctggc atcagcgcgg cgctggacct ggcagagcaa
      241 aatattccgg tggtattggt cgagaaagaa ccaacgattg gaggggtcat ggctcagctc
      301 aacaagacct ttccaacgat ggactgcagt atatgaattc tcgcaccaaa aatggttgac
      361 gcggcgcgtc agcccctcat cgaagtcaaa acattcacgg aagttgaaaa agttgaaggt
      421 ttcgtcggga acttcaaggt aaccctgcgc gaaaaagcca agtatgtgcg cgcggaccgc
      481 tgcaacggct gcggcgcctg cgcaaaagtt tgcccgattg aagtcccgaa ttactttgag
      541 atgaacctgg ctccccgcaa ggctgcatac atcccgatga gccagtctgt cccgctgatt
      601 tacaacatcg acatggacgc ctgcatccac tgctacaaat gcgtggaagc ctgcggaccg
      661 ctcgaggcga ttgatttcag catgcaggac aaaatcgtgg aagaagaaat cggcgctgta
      721 attgtggcga ccggtttcag ccactttgac ccctctccca tggaggagta cggctacaac
      781 cgttacccca acgtgattac ctccatggag atggaacgcc tgaacaactc cgcaggtccg
      841 accgccggca gtctgattcg ccccagcgac ggcaagaaac cgcagcgcct ggcgttcatc
      901 aactgcgtgg ggtcgcggga taagcgtttc gtggaaagct gctcgaattt ctgctgcatg
      961 tactccatca aaaacgccgt gctggtcaag cagatgaacc caaacgcgga tgtaaccatt
     1021 tactatatgg atatccgcac accttccaag ggttatgaag agttctataa tcgcgcccgc
     1081 tccatgggta tccattttat ccaggggcgc cccgcgatga ttaccgaaga ccccgaaagt
     1141 cacaatctat tcatccatac ggaagatatc gcgctgggca aggttgtgga acatgagtac
     1201 gacctggtca tgttaaacca ggcagcggtg ccccagccgg accaaaacca tatgagctcc
     1261 gtgttgaacg tcgcgcagtc gccaggcggc tggtacatgg aataccaccc caagctgcgc
     1321 ccgatggaca gccccacgga tggtatcttc ttcgccggag cctgccaggg accaaaagac
     1381 attcccgcca gcgtcgcgca gggttcggcc gcggcatccc gcgcctcgcg cgtcctgcac
     1441 tccaaaacat ggcaaatcga acccaccatc gccaatgtct ggtccgaccg ctgcgtcagc
     1501 gcccagggca aagcctgcgg tatttgcgaa cgctcctgcc cgtacggcgc aattgattac
     1561 acccagggga aggcagcaga ggtcatcacc gccaagtgcc acggctgcgg cgcgtgcgca
     1621 ggcgaatgcc cgcacgatgc gattacccag ctgcattaca ccgacgcgca aatcctttcg
     1681 cagatacgca ccctcctggc ggacaaacct gagaaaaaaa tcctcgcgct gcgctgtcac
     1741 tggtgttctt atggcggcgc ggacctggcg ggcaccagcc actttgaata cacatccaat
     1801 gagcgcggta tcaaggttat gtgttccgcc cggatggacg ctgacttcat ctacgaagcc
     1861 taccgcctgg gcgcgggggc cgtgctctac tccggctgcc atccgcagga ctgccactac
     1921 attaccgggc aggttatcgg cgccaagcgc gccaaacgcc tggaaacaat cttcgagcgc
     1981 atgaacatga gccccggacg cttccgggtt gagtggatta gtgcggctga aggcgataaa
     2041 tatgcccggg tgctgaacga aatgcaggca gccctggacg agattccacc ggaagccctg
     2101 cacgctgaaa tcgagcgcat gcggcccgat atggccaaac gcctgacgcg cctgccgaac
     2161 atccccggcg tgcaacgggc tatggaccat actgaagttg tcgtgaacac aattctcagc
     2221 agaaagggag agcccgtatg attagttccg gactggcaaa tgaaatcagg gcaattccgg
     2281 gtggagaaaa ccttgaaatg tgttattcct gcgggacctg cacaagcaag tgcatgatcc
     2341 agcagaaact ggaaccggaa tacaatccgc gccgcttatt gcgcatggtc atgatggaga
     2401 tgcgcacgga gagttacgcc aacccgacca cctggctgtg ttcctcctgc gacttatgct
     2461 atccggcttg cccgcagcaa attcacattt ccggtgtcat cacggctgta aaaaaaatcg
     2521 cggtgcaagc cgcgcagaag acgcccctgc ccaccgccgt ggtggaccag caaacctgtg
     2581 tggcctgcgg tctctgcgcg gaagtctgcc cgtacgatgc gattaccatt gaaacgcgca
     2641 agctgcccta ccgcggcagc atcccggtcg cagtggtaaa cgcgggttta tgcatggcgt
     2701 gcggcgcctg cggcgcggcc tgccgctcca actccatcgg gatacctgcc gatacctccg
     2761 acgaggaagt ggtcgagaat atctggtcct ggttgaaccc ggaaggagcg ctgaattgat
     2821 ggacaatcga ccgaaaatcg cgttttttca atgccagtgg tgcctctatg ctccggaaga
     2881 ccaggaatgg gtggacaaaa gcctgccaga aaacatacat ctgattaaag tgccctgcac
     2941 cggccgtatc gacccactgt tcgtacttaa cgcggttcaa ggcggcgccg acggcgtggt
     3001 catcagcggc tgtctgccgg aaaagtgcca cttcaaagaa ggcaatctgg cagcccgccg
     3061 gcagttggat gcgttttcca gcttcctgga ctacatcggc tacgatgagg cgcgcgtgcg
     3121 ctttatctgg ttggatttgc aggaccgcgg gcgcattcag cgcgagctga ccgagttcga
     3181 aagcctcctg aagaagctca acccggcaaa aagccttgcc acacgcgtac ctgaaaccga
     3241 aggaggcgcg aatgactgag ctggaacaaa aaatccgcgc ggaggcggcg cgtttgcttt
     3301 cggaagggct ggtggacgtt gtgatggctt tccgcaaaga agacgccccc ttacgcccac
     3361 agccagcctt ctttacctgc gtggaagacc tggaaaatct aatctataac ggcctgtgcg
     3421 cgaacaacct cgcaaattac ctgacccgtt acccggatga gacccgcatc ggcatgctgg
     3481 tgcgcggctg cgaaaaccgt tctgtgaacg cgctggtggt ggaaaaacaa cacagccgcc
     3541 aaaaccttta cttaatcggt gtcccctgca ccgggattat cgattggcgc aaagtggtcg
     3601 cgctgacggg ggaagacatc ctcggatatg aggaaacgga cagcagcctg cacgtacgca
     3661 ccaaacgcca aagctaccag cttccgcgcc gcgaactgct gcacgcttcc tgccagcgct
     3721 gcgtgcaccc caaccccctc agcgctgaca tcgtgctcgg ggaagcgctt cccgagggcg
     3781 acccagccct catccgtgag cgcgtggaag cctttgaggc cctcacccgc gccgagcgca
     3841 cagcctactt caagcaggaa gccgaacgct gcatccgctg ttacgcctgc cgcgaagcct
     3901 gcccgatgtg ttactgcgag gagtgctttg tggaccacaa cagcccgcgc tggacagaaa
     3961 gcggcaccac cccagccggc acgcatgcct ggcacatcat tcgcgctttt caccagaccg
     4021 gccgttgtac cagctgcggc gcctgtgagc gcgcctgccc gatggatatc cagatgacct
     4081 acctgaccga taaactgggc aaagatatgt gggacagcta ccaattcgcg gcgggcatgg
     4141 acgccaaaag ccagccgccc ttcgcttcat tcacgctgga tgacaggaaa catttccagg
     4201 ctggaggaat gcactcatga aatacatcct ccccaaacag gatctgaacg cccttctgga
     4261 gcgttggtct gctgagtacg ctgttctcgc gccgcaaaaa tccggagcgt acgcggaatt
     4321 tttgccttac actccccggt cggaactctg cctggaagaa ccgcacaaca cccgctatcc
     4381 ccccaaggcg ctcttcttcc cccagtccga agccattctg caatacaaac gaacgaccgg
     4441 acagttggag agcatctcag ctgtagcgca gaaacgggtg atttttggca ttcgtccatg
     4501 cgacgcgaaa gcggtaagcc tgttggatac ggtcttcgcg gaaggcgaca atcaggaccc
     4561 cttttggcag gcccgccgcg cggaaacgct gctcatcggc ctgggctgcc atgcaccctg
     4621 cgcgacctgc ttctgcacca gcgctgaatc cggacccttc cacacagatg ggctggatgc
     4681 ccttctgaca gacctgggag aaatctatct ggtggaactt ctttcagaac gcgctggcgg
     4741 cctgttcgat gctctggcgc ccgccagctc catcctgcag gagaatgctc aagctgtcca
     4801 atcagccgcg gaatgtgccc tgcccaaggc ttttgaactg gacgggttaa aaggcaaatt
     4861 ggaccgcgtc tttgaccagg atatttgggc gcagctggcg cagtcttgcc tcggctgcgg
     4921 catttgcact ttcctgtgcc ctacttgctt ctgctttgac ctggtggacg aagcgcagcg
     4981 ggatacgcgc gtgcgcaact gggacacttg catgttccgg gtgtactcgc tggaagcttc
     5041 ggcgcataat ccgcgcccga cgcgcaaaga acgcacccgc caacgcctga tgcacaagta
     5101 cagctactgg tgggagcacg ccgacaaatt gggctgcacc ggctgcgggc gctgcgtgcg
     5161 ctactgcccg gtcggattgg atatccgcgc gatgattcgc acagccggcg cgtacgaaaa
     5221 cgaggtgctg catgccaatt aatacatcta cgcgcaaccc ataccagccc acacccatgc
     5281 gcgttttgcg cgcgtattac gagtccaccg accgaagctt gaaaacgctg gagctttcct
     5341 ttgaaaacca cgcggacaga caggctttct tccgcaagtt taacccgggc cagttttgcc
     5401 aattatcagt ctttggcaaa ggcgagtccc cccttggcgt ggcctcggct gcctgggaag
     5461 gcgattttgt gcgctttacc atccagcgca tgggctcggt caccaaagct ctgcacgaac
     5521 taaagcccgg cgatacactg ggtatgcgcg gacctttggg caacggcttc ccgctctcgg
     5581 agtggaaagg caaaaacctg gttatcattg gcggcggctg cgcgttttcc acgctctatg
     5641 ccctcaccaa gcacctccaa taccctcaaa accgcccgga ctacgctcag ttggtagtta
     5701 tttacggcgc gcgcagctcc gggctgtgca tgtacaaaca ggatatccaa agctggtacc
     5761 agcgcgaaga catggaagtc catcaggcaa tcgacgttcc cgaagagagc tgggatcacc
     5821 acgtcggata tgttccggat gtggtcaggc aaatcgcgcc ctctccagac aacgcggtag
     5881 caatcgtgtg cggcccgcca atcatgacca agttcactct gccagcgctc gtctcgctgg
     5941 gtttcccagc ggaggcgata tttacctccc tggaaaaacg catgaaatgc ggcttgggta
     6001 agtgcggccg ctgcaacatc ggttcgaagt atatctgcaa agacggtcca gtcttcagtc
     6061 tggcggaact caacgcgctg cccgcggata tttaatggag atagcatgag tcaacgcgga
     6121 attccacaaa aatcaaaaaa tgaacacagc atgaccatcc gccgcggcat ggtcacgcgc
     6181 aattacctga tgacctggga cctggataaa tgcgtcggct gccagattgg gccgattgtc
     6241 tgcccgaagc aagccctgac gcacgtgccc gtccagctgg aaaacggccg catcgccgtc
     6301 aaagcttccg tggatgtgga cgagaagaaa tgcgtgaact gcggcatgtg tgtcgtctcc
     6361 tgcccgacgc acgccatcag cctgaccatc aacggcaaaa cggaaatccc ggtgcttgaa
     6421 tatgaaactt atccggagta cctctcgaaa accatcttcc ggcgggaact tttcgacttc
     6481 agcctcaaag attttgtcat agataactgt tccgccaacg tcatcagtta cgatgagacc
     6541 cgcgacacga tgcgtgtgga ttttgaaaac tgcatccact gccgacagtg cgaaatcgca
     6601 agcaagggcg cttttgaagt gcgccaagcc tggcagggtt cggtagaact gcaccgcgag
     6661 cgctgcgtgg aaggctgctt tgcctgcgcg gatatctgcc cgacccgcgc cctgcatatc
     6721 gacgaccagg gcgagctcgt cctggcggat tactactgca tcaagtgcgg agcttgcatg
     6781 caggtctgcc cgatcaaacc caaatacgag agccaggaat tcgaatttga gtatcagggc
     6841 atgcaagtca aacgcagccg cgatgtgctg ctgaacgcag acgaattggc agtcaaagtg
     6901 gaacgctggc ggctcaacca taaacccgtt tccagcgctg cctggaccga agccctgcgc
     6961 aagttggcgg acgacaacgc gaaaactgtg gagattgacc gcaaacgcgc cttacgccgc
     7021 gcggacctgg tgcgcgcgct gcgcgggcac accctgaccg aaacgcgccc ggtggcaaag
     7081 caaagtaagc aaagttaatt cttcttcagg gtgtgcaaga aatgacgaat tgcaaaccct
     7141 tacgcggttc taaacgctgg ggctaatggt gtttttatag aggcaagcaa atgtcaaacc
     7201 gccttgttct tcttggcaag caattaattt ccgacacttc ttctgctgaa atccgccgga
     7261 ttgaagatct gctcaagcag gagaatattc cctaccgcct tcaaacccag cgctcccgcg
     7321 gctccgtagg ttctttcatg gactcccgct catatgcggc gtcaaatatc gcgctgtaca
     7381 aaggggcagc aacgccaacc tttgtgtatg ccatctatgt gcgcccaaaa gattacaaac
     7441 gcgcccaaag tctgatgcag gtgcgttaat ttctggccgt tcccccggga aagtggtttg
     7501 agaggggaat tacctctcag agccgtatcc gaacctgctt gaataatcaa atgaccagcc
     7561 cgtttcccgc gcgtactcgg gattcttctc cccgggtccg tagatcagca cgcgcgcctt
     7621 cgagcgcaag cgcccgaaac ttgagctgtc cggtgcggcg ctaatcacgt tgcggaaggt
     7681 gccgctgttc aggtaatatc tctcccgccc cccggaagtg cgcacaagct ctactgcggg
     7741 agtgtgcgta tgcccgctga ccatgcaggt tacggtggaa tcgctgcggc taaggcattc
     7801 ttctttctgg atcatcccca tcagtgaacc cagcgtatac ttccctgaaa gcgattcaat
     7861 cagcttttcg aaaacccaaa agggaatgcg gcgcttccag atgcgtttat ccagcaggat
     7921 tttcagggtg aaaaggggaa gcccgaccag cgctcccaaa gtcagaaact gctgcttcag
     7981 ggcttcatcc ctcgcaatct cgtcacgaat ccacttgaac acgggttcca ggtagcccca
     8041 ggtttcatca cggttgagac ccggagtgga aaacagaaaa ctcatcagcg cgttggctgg
     8101 gcggacatcg tcaaattcca tgatgcgctg ataaagcagg agcagcggag tttttttcag
     8161 gatgccctcg gctgtgtaat agtccttaaa gacttttggc agcctggcag ccagttccaa
     8221 ggtggtaata tcgcccagca ccgggcgttt gtaccattcc gcgggcataa acagcggcag
     8281 tgaggcttcc gcgctgaggt taatcccaaa attgttcggg tcgtactcat gcccatgccg
     8341 caccaagacc ccgggctgac catcgaacgg atgcagatac tggttatcaa acggcgcggg
     8401 gctgtccgct atcccaagca tgcgcctcac ggcggaacgg actgccggtg tggcattggc
     8461 caggcggtcg tggttgcctg ggatgaaatg cactctggtc gggcgttgga agctgttttc
     8521 cagactgcga atgctttgca aggtttcagc tacgcgcgcg tcttcagcaa tttctttcag
     8581 gatggccaga atcttggctt cccgggcgct gccctcagcg atgtcatcca gatgaccgta
     8641 aggccgcaga ctgtcctcca gccacgaggc ggagcgggtg agttcgaaaa tatcgccggc
     8701 aagcacaagg tccacttcgc caaccacgcc gtcatccaaa gactccgcga tttcacggaa
     8761 gtactcctga tagaccccgg cgggaaggtt gtgattgaac tgcgtactgc ccaatcccag
     8821 agacttcgac tccgccagat gcagatcgga catgacaatc agcattttct acctctttat
     8881 tctcgtgaag cccgggtatg cagccgcacc ggaaatacca gaaacgccgc ggaaaacacg
     8941 gttttttact ccccgctcca acggaatgcg ccggcttcgc gtgtgccaaa caagacccga
     9001 gagctgtccg ctgggtcgag aatgacgctc tgataggacc agcgggtcag cacaggcgtg
     9061 gcaagctgcc aatgcgcgcc gtaatcgccg ctccagaatg tggaacccgc cccacctgcg
     9121 gccatcaggg ctgtgttctg ggcgtggaaa ctgatggaaa ggatgttatt gcctgcggcc
     9181 cccaccggta tccacagcga gccggtgtta tcgcgcatgt agatgccgtt aaccgtggcg
     9241 gcaaagtaat cgtcgcctct ggccagtatc tgattggtgt taatcccttc caaaccctga
     9301 ttgatgctgg tgagggctcc gtctttcagg tagcgtgctg tgcagattcc ggaagaatcg
     9361 caggcggtca agagcacccg gccaagcgct gtatcgactg agaggctggg gatggaatag
     9421 ccgttcaagc caacctgggt ccatgaaccg cccgaatact tccacaaacc cgcccccgcg
     9481 ctgccgccga aaagcgtgct gccgatggct gtcaggctga tgagcggcgt gctggtaagc
     9541 gcggcttcgg tctggacttc cagagcagtc aggatctcct gcgcggcgtc gtcttccggc
     9601 tggctgatcc ctgccaggac ggtctcgaga taccagctcg ggtcgtaacc cgcggattgg
     9661 ggattattaa tcgtagtcca gcttcctccg ctggaaacgt acaccgcgct gggagtcagc
     9721 gcgtagagct tattgttcgc cgcggtcagc caggtcacca ggcggcttcc aaggccggaa
     9781 ttcacctcct gccaggtagt gccgccgtca ttcgtgcgaa ccacgccgat gccgtagacc
     9841 cccgcgtaaa catagcccgg ctgagctgag atagaggcaa acccgttgat cgaatggctg
     9901 ctcacctcac tgtggctggg ggcccagtcc gctccctgat tcgttgagcg gtacagtccg
     9961 cggtaactca agccggcgta ccagttttgc ggggaaccgg gcgcgatggt gaaatcgtac
    10021 aggaactgac cgctcaaccc cgcgctgccc caactgctgc cgttgttgct ggagcggtac
    10081 agtccgttct cgtatgtaca ggcaaacacg ggtttggtcc cgcttacaga ttggtccaaa
    10141 tacaagcggt agacttccac gtcagttggc aaaccgctgc tgaccctcga ccagctcgtt
    10201 ccgccggagg tgcttctgta gacgccctcg ccgtgccaga cgccgccaaa aatcaaggag
    10261 gggttgccac tgtcgagcgc catactgcgc gcgctgaggt cggaaacgcc gctgttaatc
    10321 gcgctgaaac ttgcgccgcc attcacgctc ttataaaagc cgtgctcgtg cgccgccagg
    10381 tacagcacac tgctgttggt cgggtctacg tcaatgtcat agaagtagtc gtcggtattg
    10441 aactggtcac cgcgcagcag caggttccaa tccaggccgc cgttccagct ctgataaacc
    10501 acgccaatca gggtgttctt tatcctgccg gcaaagtaga ccatggacgg gttttgcggg
    10561 tcaatctcca ggtcgtaaac tacaatcgga tcggtcaggc tgccgttcga ggcaaaccag
    10621 ttggcacccg catcggtgct tttgtacacg ccgccggtat aggttccggc gtaaagcaca
    10681 ttcccgtttt gcgggtcaac agccagcgat tggatgaaag tgtttcccag cccgttgatg
    10741 cgctgaatcc agctgacgcc ctggtcggtg ctgcggtaga cgcccgcgcc gtaagtgccg
    10801 agatacaaaa tggagggatt gcccgggtct accgcgacgg ctgtgaatgt gcccccgtcc
    10861 ggaccgatgg acatggattg gatggaaggc gggtccggcg ccctgaacag gatgggcaga
    10921 tagataacaa cgccgccttc cgcgctctgg gaattactcc ccgccgaagc aggagccaga
    10981 ggcgctccag cagcgaggac cacgcaaaaa aacaggaaaa tcattcgggt ttttttgttt
    11041 tttttcatca tgccggccag gcagcccttt cgttagtcaa gctgaggaat tgcaatgatt
    11101 atgccacctt tcttgccggt cggtagaatt tggagttaat tctggcggtt tatcacccgc
    11161 gaaaatcccc ttatgcaggc tttcagcgct tcgtcatcca catcgtgatg cgtcaccagg
    11221 cggaatgtcc gtgcaccggt agccccaatc aacacccctt gctctgccag gagcgacacg
    11281 atttcagcgg catcctggcg tacctcaggt tccagagcca ggtagaccat atttgtgtaa
    11341 ggcgttcctt tatctatacg cagccccgga actgccctca gcgcgtctgc cagttcgcgc
    11401 gccctttgat ggtcctccgc caggcgctca atcatgtggt ccagggatac caaacccgcg
    11461 gctgccagaa tgccggcctg gcgcatgccc ccacccagca ttttgcgcag gcgtcgggct
    11521 ttgctaatcg tttcccggct gctgcacaag actgagccaa ccggcgcgca taaacctttg
    11581 ctcaggcaaa aggtgatgga atcggcggac cgaaccagct ctgccgctgg cacccccagc
    11641 gccacagcgg cattaaagat acgcgcgccg tcgatgtgca aaaccaatcc gtgcagcctt
    11701 gcgaaagcag ccacgctgtc cgtgtattcc gcgctcagcg ggatgccccc gcaggcgttc
    11761 tgggtgttct ccaggatgac catgcgcgaa agcggctcat ggaaatccgc ggggtcgcgc
    11821 agcgcgccat gcaggtcttc cagcgccagc gtgccgtcgg attggttggg aatggtgtgc
    11881 atcgacacgc ctcccaggac ggaaaccccg ccggcttcgt gcaggaaagt atgcccctgc
    11941 ctgcccatta tcacttccgc gccgcgctcg cagcccgcca ggactgccag caggtttccc
    12001 atcgtcccgg aagttacaaa cagcgcggct tctttcccga gcagctccgc cgcgcgggct
    12061 tccaggcggt tcacggtggg gtcttcacca taaacgtcat cccctacctc agcgctcgcc
    12121 atcgcctggc gcatccgtag cgttggtctg gtcacagtat cggaacgaaa gtcatactgg
    12181 ctcatacatc ctccatagca gataaagtgt tgagaacttc tttcaggtca tccggcaggg
    12241 ctgcctgaaa ttctgtcagg tctttctctc ccggaaggat aatccgcagt ttcgcggcgt
    12301 gcagaaagaa tcgctccaaa ggaaaagagg ctttgcgcct gccgtagacg cggtcgccca
    12361 cgaccgggca tccaagatac gccaggtgga cgcggatttg gtgtgtgcgc cccgtgaacg
    12421 ggcgcacttc caacagcgta tggtcgcgaa agctctgttt tgtgaaatat tcgctgaccg
    12481 ctttccggcc tttgccttcg tatactaccg ccatcttctg ccggaatttc gcgtcgcgtc
    12541 cgaccgccgc ttcaatgcgt ccgttgggcg tcggcggatg tccgtcgacg agagccaggt
    12601 agtacttttc agttttgcgg gatttgaact gccgtaccag ccaggcgtaa gcgctttgtg
    12661 tgcgggccag caccagcacg ccagaggttt ccttatccag gcggtgcact accccgggac
    12721 gctctggctc gcctacctgg cgaatttccg gccagcggta aagcgcggcg ttcactacgg
    12781 tgccggtgct gtgcccggct cccgggtgca caataaggcc ggcgggtttg tttatcacga
    12841 tggtgttctc atcgatgtaa atgacgtcca gcggcaggtt ttcggcagga atgccctgcg
    12901 cctgtggtgc cagcaattcc acgctgacca tttgcccggc gctcagtttt agagcaggtt
    12961 tcaggcatac ttgcccgtcc acccaaactt tcccgctggc aatcagctcc tggaattgtg
    13021 agcgggaatg ttcgggcaga acttccgcca gaaatttatc cagccgctcg ggctcagact
    13081 gctcgaagcg gagctctttc gtctgtgcgt tcatgcatgc gcctgttcta ccctttgctc
    13141 tcttcccggc tgttttccgc gtcaggcaag ctgtgtgtgc gcgctttctt atcttccagc
    13201 agcagcgcga ggatgagcag cccgaccccg acggtcacgc aggaatccgc tacgttaaac
    13261 accgggaaag aacctaccgc gataaaatcc aaaacgtaac cgaaattaaa acggtcaatc
    13321 aggttgccga gcgctccgcc gagcataatc gccaggcaga tgcgcacgaa tttttgctcg
    13381 taaggtatcc tctggtagta aaacagaatg aacaggatga tgaccgaggt gagcaccttt
    13441 aacaccatgt tcccgtcctg gaaaatcccg aatgccgcgc cggtattctg ccagtagacg
    13501 aagcggaaaa actccgaagg cgcgaaagga agccaggcct gacccaaggc caggttctgg
    13561 cggatgataa atttcgtggc ctggtctaca agaatgacga ccaaggcaat ccccaaaagc
    13621 agcaggtttt gcttaagttt ggttttcaaa cggaattccc tctctatctg gttttttgga
    13681 tgcgcagggt tagctgctca ttgtcaaaac tgtcgctggc ggtaatccag ctgccggtat
    13741 cttcgacctc cgccagctcc accgcaagcg tttcagcctt gatcaggtcg gcgctgctgc
    13801 gcactgcctc agcgagctgg ggggtggcgc tgtaatgcaa gcggatgcgg tccgcgatgt
    13861 ccaatccgcc atccttccgc aaagtctgta cgcgccggat gaattcccgc gccagccctt
    13921 cataaaccag agcctgggtc agatggatat ccagcgccgc caggtaagga ccctcggacg
    13981 ccacttccaa gccttcccgc gcggaagtgt gcacttccac ctcgtccggt tgaatttcat
    14041 agtacttgcc ttccacctgt acgaccagat tgaggccgtc caacagtttt tgagcgcttt
    14101 cctgcacagg cagttccaga atggcattgc gaatttgcgg gaagaggttc ttgtacttct
    14161 gaccgagctg cctgggcagc ggattgatgc ggtaatcaac aacctcaccc gcgccgctga
    14221 gcaaacgcac ggatttgaca ttcagctcat ccatgagcac atccttaaag cgttccacga
    14281 tttccgcgtc ttcggccgaa ccgaccgcga aggctgcttc cgccaacggt tggcgcactt
    14341 tgatgctgga cttgttgcgc gcggcatgcc caagcgacgc caatcgcatt actagcgcca
    14401 tatcacggtt aagtttctcg ttgatgcgct gcgggtctgc cttaggccac tcaatcagat
    14461 gaaccgaaac cggcgcgtcc ttattcacgc tgcgaaccag gttctggtat agttcctcag
    14521 ccatgaacgg catggttgga gccagcaagc cggcaagcgt ggtaagcgcg ctatagagca
    14581 cttcatacgc ggcggcctta tccccgtcag attcgctctt ccagaaccgc ctgcgcgagc
    14641 ggcgcagata ccagttggaa agctgatcca caaaagcctc gataggtcgg gtcgcgccaa
    14701 gcgcgtcata cgcttcatag gcggctgtca catcctgcac cagcgtgtgc agcgaggaaa
    14761 gcagccagta gtccatctgg ctgtattggg ggatgacgtc cagttccggc tgccactgat
    14821 ccagattggc ataagtaacg aagaacgagt aggtgttcca cagggtcagg ctgaaagttc
    14881 gcagcacttc cccgaccagg tcggcgctga agcggcgttc attcccggac gggctggagg
    14941 tgtacaggta ccagcgcacc gcgtccgcgc cgtgctgatc aatgacttcc cagggatcaa
    15001 ccacgtttcc gcgggatttg gacatttttt gcccttcgcc atccagaata agccccaggc
    15061 agaccacgtt cttgtagctg atgctgtcga agaccagccc gctgatggca agcagagaat
    15121 agaaccaccc gcgcgtctgg tcaaccgcct cgcaaatgaa atccgcgggg aactgctgtt
    15181 tgaattcctc aacgttctca aaggggtagt gccactgcgc caccggcatc gcaccggaat
    15241 cgaaccagac gtcgatcaat tccttgacgc gccgcatgct gccctggccg caatccgggc
    15301 agtcaaactg tacggcgtct ataaacgggc ggtgcagatc catcccggaa aggtcgcgcc
    15361 cggccagctt gctcagttcc gcgacggaac caacgcaaat ctgcttgcgg cagtgcgagc
    15421 attcccacac gggcagcggc gtgccccaat aacggtcccg ccctaaagcc caatcgatat
    15481 tgttttcgag ccagttgcca aagcgcccgt tcttgatgtg ctccggcacc cagttaatct
    15541 cctggttcag ctgcacaagg cggtctttaa tcctgctcgt gcgaataaac caggtcgggc
    15601 gggcgaagta gagcagcggt gtgccgcagc gccagcaaaa tggataactg tgcgtgtacc
    15661 gcgaagcctt gagcaaaagt ccgcgcttgt ccaggtcttc gatgatgccg gcgtcggcgt
    15721 ccttgacaaa catgccagcc cacggggaaa cttccttttt aaatttgcct tcatcattga
    15781 tcgtcatcaa tatcggcaga tcgtattctt tggccacagc catgtcatct gcgccaaaag
    15841 caggcgcaat gtgcaccaga ccggtgccat ctccagtggt gacgtattcc gcgtttacca
    15901 cgtagtgagc cggcttgtcg gtagggagga aagtgaacag tggtgcgtag cgtttcccct
    15961 tcagcgcccg gcccttcatg gttttgagaa tctttacggg cgtatcgcca aacacagcgc
    16021 caatgagctc ttttgccagg attaagcgct cgctgctgag tccatccgca cgggagacaa
    16081 gcgcgtattc cagctccggg tgaactgcca ccgccacgtt gccgggcaaa gtccaggggg
    16141 tagtcgtcca gaccagcagg tacgtttcag cttcgtccac cagtgggaag cgcacgtaga
    16201 ccgacgggtc ttccgtctcg tggtaaccct gggcaacctc gtggtctgaa agcggggtcc
    16261 cacagcgcgg gcagtatgga accaccttga aaccttgata cagcaggtct ttttcccaga
    16321 tggatttgag cagccaccac acggattcga tgtactcgtt ccggtaggta acgtaagcgc
    16381 ggttaaggtc cacccaataa ccaatgcgtt ctgtcaagcg ttcccaatcc tgaatgcgtt
    16441 cgaaagccga ttcgcggcaa agcgcgttga atttttcgat gccgtaggtc tcgatttgcg
    16501 acttgttggt gaaaccgagc ttcttttcga cctcgatttc caccggcagc ccatgcgtat
    16561 cccagccgcc gcggcgatca acgtaaaaac cattcatcgt cttatagcgc ggaaacatat
    16621 ccttgaaaac gcgcgcgagg acgtggtgaa tacccggctt gccgttagcc gtgggcggac
    16681 cctcgaaaaa gacatagggc gggcgatctt tacgctcatc ggaagactat ttgaacacct
    16741 cgttcatctt ccaaaagcgc agaatgccgt cttccaggac tttcacattc agttttggcg
    16801 aaacagcgtt aaacatactc ctccaaaaaa aagcccgtcc caaatcaggg acgagctcaa
    16861 actcgcggta ccaccctggt tcccgggaaa tcccgggcaa ctcagcagct tcgcccacac
    16921 gggcaaacgc agccgccact aacgctgcag cagcgcggct cccttactga cccgagggcg
    16981 ttcggttcgc ggctccggaa ggattttctg cccgttgttc cgccccggct tccaccattc
    17041 cgggttcgct gtcggattca tccggcatac tcgtttccat caatgctttt aacaaagagg
    17101 gattatccca taaaaacagc ttcaggtcaa cctaagcaaa gaccgaacct aaacaaagac
    17161 tgaaggagcg caaagcaaaa tctggacgcc ggagggtttt cccagcgcga atcgaagagt
    17221 attcccgttg ttgcaacagc ttctttcagt aacaagcctc tttctcctct ccttgctccg
    17281 tctactgcta gtgaaagaat caaaaacacc ctttgcaggg tgtttgggtg cctggacggt
    17341 ttcgaaccct caacctcctc atccacagtg aggcgctctg ccgttgagct acaggcacca
    17401 tataggcggt cattctatca cagcgcttgc ccctacgcaa ggatttctgc caggcgtttc
    17461 tccagttcgg cttcgggtac gacctgctgg ctgtggttcg ccaaatcctt caagaccacc
    17521 tcggatcggt cgatctcatc aggtccaatg accagcgcgg cgcgcgcgcc cagcttgtca
    17581 gcgtacttaa gctgcctggt tagtttatcc gctccgggat aggaaaagac cttgaaaccc
    17641 gcccgtcgca gacgcgttga gacgcgattg gatgcgccgc ggcagttttc attaaaaacc
    17701 gacacaaaaa cggcctcacc tgccgcgctg atttcaggaa gcagccctaa cgactccagc
    17761 agcagcagga taaccacatc gcccatcgcg aagcctacgc cgggcagagg cctgccgccc
    17821 acgtcgccga cgaggttcgc gtaatgaccg cctcccagga tagcccggaa gttcccggcc
    17881 gtgtcgcgcg cctcaaagac tgtgccggta taataatcca gcccgcggat gattttcggt
    17941 tcgtaagccg cgtattcccg cacgcctaat ccttccaatt cagtaaaaag cgcggtcaac
    18001 tctggggact gctgccacag gtcggcgctg cccaggagca gcttcagctc gcgcagctgc
    18061 ccggaagtta aacccaggtc aagcgcgttt ttatcccaaa catccgccgg ctgcttgtcg
    18121 atgcggtcaa tctggcgcag cacagccggt cttagttcca gcgggatgcc gatgcgcgca
    18181 agatgtgcgt ccatcagccg gcggtcgtta atcaggattt tcacctggtc cgggcgcaca
    18241 cccacgctgc gcaggaattc cgctgccacc gcgataagtt cggcatcagc actcacttca
    18301 tccgagccga tcaggtcaat attccactgg aaaaactcgc gcgtgcggcc tttttgcggg
    18361 cgctcgtagc gccaaaacgg tccaaaagac caccaccggc aggggaaagt cagctcattc
    18421 tgcttcgagg caatcatgcg tgccagggtc ggggtcagtt ccgggcgcag ggcgatattc
    18481 tccccgctgc ggtcgggaaa gacaaaggcc tgctccttca ccaattcctc gccggatttg
    18541 gctgcataca agtcgatagt ctccaggcag gggccgtcat actcctggta accgaacgct
    18601 gccgagacct gttggattct ggaaatcagc cagcgccgga cggccatctc ttccggataa
    18661 aagtcgcggg taccctttac gcttcttaca attttttcca taacgcattc ctttaacaat
    18721 tgtcgttaaa gtatatcatg gggaaaatcg ggtaaaatga tggtaataac gggtcattct
    18781 caaggaaaag cgatgaaaat aagcgaaatt ctggaaattt cccagggcag tttgctcacc
    18841 ccctacgcaa atcctgacat tgaaatcctc ggcggcttcg ccggagacct gatgagcgac
    18901 gtcctggcaa gcatccagcc ggaatccgtg ctcatcacgg gcttaagcaa cccgcaggtc
    18961 atccgtacag cactgattgc cgatgtgcgc gtggtaatct tcgcgcgcgg aaagcagccg
    19021 gctcaggaaa ccatcaaact ggcaatcgag gagaaattac ccgttatcag ttctgatctc
    19081 ggtctctacg agatcagcgg gaggttgctg cagcacggcc tgcccagctt tgaaaaacgc
    19141 atgctgcacg ccttcgacag tgattgagcg cgaatatgtt ctccgataaa cctgctgtta
    19201 ctgacgacga agccgcgaaa atcacccgcg tggaggagct tgcctacgag ctcagcgtcg
    19261 gcgaagtgat gaccagcaag gtgttctcgt tgcgcccgaa tcaaacgatg cgcgaagcgc
    19321 tggatgagtt taaacaacac aggatttccg gcgcgccggt tgaggtcgat ggaaagctgc
    19381 tcggtatcct ttcgattgag gatatgatcc gctccttacg cgacggccgc atcgacctga
    19441 cagtcgcgga ttacatgact gcggatgtca tcaccgcccg gaaagaggac ccggtaatcg
    19501 aggcattaaa gctgtttgtc aagtcccgcg tgggcaggct gccggtgctt gacagcgatg
    19561 ataaactagt cggtatcctg accaagagcg acattaccaa cggactgctc aaggcgctgc
    19621 aaaacgacta tgaagccgaa gagttgatcc gttatcgcgc ttcgcacctc ttcgaagata
    19681 tcgtttccga ccgcagcacg cttacgctgc gttataacgt caagaggaac gatttttccc
    19741 acggaggggc tgcctccaac aatattaaaa aagctctgct caggctgggc gccaccccgc
    19801 aaattgcccg ccggtgcggc atcgcggttt acgaagcaga gatgaacctg atcatccaca
    19861 ccaaccacgg cggcgtcctg cgcgttgaaa tcgaaccgga ccgcatcacc atggaagctt
    19921 acgacgacgg ccccggaatc ccggatatct cgctggcgat gcagcccggc tactccaccg
    19981 ctccgcagga aatccgcgag aaaggctttg gcgccggcat gggtctggtc aatattaaaa
    20041 gctgcgtgga tgaaatgaac cttgcttcct cgctggaccg cggcacgaac ctctatatgg
    20101 tcatgcacct ggataaaacc aggcattttt cctgatagaa gctgaactat gcgcctaaac
    20161 gaaattcaaa accaactcca actaaagacc ctcaccgcgc cgaccatttc cgacaacgtg
    20221 gaaatccgca caggttacac ctcggatatg ctcagctgcg tcatgaccgg agcgctgagc
    20281 ggcagcgtat gggtgacgct gatggcacac aacaacatcg tcgcggttgc ctccctgctg
    20341 gatattcccg ccattatcat tactgagaac gcacagccgg atgaactcac cattgccagg
    20401 gcacgcgaga gggaaattgc gttgttctcc actgccaggc cgacctttga aatcgtaggc
    20461 aagctgtggg agctgggtat ccgggcagag gaatcgtgag gttccctccg tgattgaatt
    20521 tcgcgctgaa ttgcacgtcc ataccgtcct ctctgcttgc gccggaacgg acatgctgcc
    20581 cgggccgatt gtggaagcag cgctcttcaa cgaaatcaat ctgattgcca ttacggacca
    20641 taatgccggt tacaacgctg cgccggtgat aaaggcagga cgcagacaag ggctgatggt
    20701 gctgcccggg atggaactgg aaaccgagga agaagtccac tccctgtgcc tgttcgatac
    20761 gctggaccaa ctcgacgcgc tgcagaaaat tgtcgatgcg aggcttccgt tttacccgaa
    20821 caacgaagat ttctatggcc cccaatgggt ggtggacgaa aaagggaata agcttcgccc
    20881 ggagacaagg cagctgctct ttcccgtcca gatttctatt caacgagcct gccggattgt
    20941 gaaggacctg ggaggtctgt tcatcccagc gcatatcaat cgggaagcca acggcttgct
    21001 ttcgcatctg ggcacggttc ccccggacct ggatgttcag attcttgaaa tcaaccgtca
    21061 cacgaccaaa gaagcctgtc taaaaaagca ccccgaactg gcgcgctacc gcctgataca
    21121 aagcggagac gtgcactacc tggacggctt cctgggtgcc agcacgttca gggcgaaaaa
    21181 cagctcgctc aaggcgatca cagaaggatt gctcagcagc ctctgagagg ctcctggtcg
    21241 atgtattccc taaaattaaa tggataagat gctgataagg aaagaactga ccaatcctca
    21301 ggattttttc aaagggttca gtttgttttg accaacaagc ctacgaacag cctttccccg
    21361 cttccttccc ggcggactca gcttatctta tgatgctgat aaactttcgc tgggacgggg
    21421 aatttacatt tgaggctgcc caacaagaac tggttattct tttgggacag cccaatgtgg
    21481 gagatagcac ctggtttagg cctcgtggtg agcgcgcaaa tccgcaaatg cccataaaaa
    21541 ttgtcatgga gtgggaaatc agaaggagcc gtccgacttt tgggacagcc ccgaacattt
    21601 ttccttcccc agcggatggt tcctccacaa aaaaaccgct tgccgctggg gaagtttcgg
    21661 gatttgggta cccgtggtgc cttatggagc atgaggctgc caaaacgaaa tcatcctgaa
    21721 agtcacattt tgttcttcaa caccctgctt aaagtcattg acctaatcgg ttttagggta
    21781 taaactaatt gcatgttttt tcacaaatta atactatttg tttgccttga ggagtactta
    21841 tgaccccgtc tgaaaacaat gtatctgcca atcccctgga gaatcccgaa caaaaagcga
    21901 taattgacgc catcatcgat gaaaaccttc atttgaacgg cgccaccatg gtgattctca
    21961 accaggttca ggaaaaaatc ggctatattt ccaagcccgt gcaggcttac attgctgata
    22021 gattgggagt gcctgtttcc aaggttcacg gcgtcgtttc gttctactca ttcttcacca
    22081 cctccccgcg aggaaaacac accatcaagt tctgtcttgg aaccgcctgt tacgtgggcg
    22141 gcatcgatca gattatcgag aaagcaaaac aactgctcaa tatcgaaccc ggaaatacca
    22201 ctccggacgg gatgattacc ctggaagtat gccgctgcgt cggcgcgtgc agccaggcgc
    22261 ctgtagtcgt gatcgatgac aacatccgcg gccgcatgca accaaacagg ttcccaaagc
    22321 tgattcagga catacagacc caggagactg ctcattgaga gaaattgccc tgcatatttt
    22381 ggatatcgct gccaacagca tatccgcggg cgccaggaat atcgcaatca gcatcgtcgg
    22441 aaatcaccgg gaagacagcc tggaaatcac aatcactgac gatggaaaag gaatgagccc
    22501 ggagatggcg gcgatggtga cagacccatt caccacgagc cggaaggaac gcaatgtagg
    22561 tttgggtatc cccttgctca aggctgccgc ggaagcctgt aacggatcgt tcaggttagc
    22621 ctccgagccc gacaagggaa ccaaggtgca ggtgagcttc cagctgggac acatcgaccg
    22681 gatgcccctg ggcgacctgc cgggcacttt tttaggatta ctgatcggca cgccggaggt
    22741 ccactggact tttcgggtga aaaatgatga aaaagagttt accttcgatg acgcggaatt
    22801 gaaaaaagaa ctgggcgagg actgcctggc tgaacccgct gttattcgat atgtacgtga
    22861 actgtttact gaaggaatcg aagcgttagc gttgaattaa ggagagagct atgcctacca
    22921 ttaaatccct tgaagatttg aaaaaaatgc gtgaagaagc tttgcgcaaa caagagctga
    22981 agaccaccac cggcaaaaag gaaatcgtgg tcgggatggg aaccgtcggc attgccgccg
    23041 gcgcccgcga aacgctgaaa gcaatcctgg agtttgtgga cagcaaccac ctggagaaca
    23101 tcatcatcag gcagaccggt aacatcggga tagattcttt cgagccggta gtccaggtta
    23161 tcctccctgg ggaagaaaag gtcacctacg ggcacgtctc gcctgatgtc gtcaagatca
    23221 tcatggaaga acatgtgata aaaggcaaaa tcgttgcgga ctaccaggtc aagagctaag
    23281 cttcctgtgg tctggcgata tcttggaaat tactgagcgg aaggaatatt atggcgttct
    23341 atagatctac tgtgcttgtt tctgtcgacc cggtgtgcgt ggagaaaggg gcttatgagt
    23401 taattgacgc gttgaacgac gagttaatta aacagggcct catcgacgag gtgcgcattc
    23461 tggagacccc acggctgggt gatccgcaaa aattcggtcc ggatatggtc gtctaccccg
    23521 aaggaacgca ttataccaac ctgaattact atgagattcc ccgcctggtc gaagagcact
    23581 ttattaaagg acgcatcctc aagcagtatg ccgcaaaaat gcaggacgcg attgatgagg
    23641 agctcagcgc gccgaaatcc aaggagattc gcgttgttct gcgcaacgtt ggcgtgattg
    23701 atccccgcaa tatcgaagac tacattgcca atgacggtta ccaggcgctt ggcaaagtat
    23761 tgaccgagat gagcccggaa gaggtcattc aggtcgtgtt ggatgccaag ctgcgcggcc
    23821 ggggcggcgc tggcttcccc accggtctca agtggcgctt cgcccgtcag gctgccggcg
    23881 atatcaaata tattgtctgc aacgcggacg aaggcgaccc cggcgctttc atgaaccggc
    23941 gcgtgctgga aggcgatccg cactcggtcg tcgaaggcat gatcattgcc gcctacgcga
    24001 ttggcgcccg tcaggggtat atctactgcc gcgcagaata tcccatcgct gtcagcacca
    24061 tgaacctggc aatgaaacag gcgcgtgcgc ttgggctcct gggtaagaac atcctgggta
    24121 cagacttcag ctttgacctg gagatgcgca tgggcgccgg ggcatttgtg tgcggtgaag
    24181 agaccgcctt gatggcttcc atcgaaggcg gacgcggcga gccgcgtgtt cgcccgccct
    24241 tccctgcgca aaaaggcctg tggggcaaac ccaccaatat taacaacgtg gaaacctacg
    24301 ccaacgtgcc ccagataatc ctcagaggcg cggaatggta cgccagcatg ggcactgaaa
    24361 agagcaaggg caccaaaacc ttcgccctgg ccggtgatgt aaagaacacc ggtctgattg
    24421 aagtcccctt tggcatcaaa ctgagcgaaa ttatctacga cgtcggcaac ggcatcaagg
    24481 gagataaaaa gctcaaagcc gtccagaccg gcggcccaat gggcggttgt atccctgctt
    24541 cgatgattga cctgcctgtg gactatgaaa ccctcggtca ggcaggctcg attatgggtt
    24601 ccggcggcat gattgttatg gacgaggata cctgcatggt ggatatcgcg cgattcttca
    24661 tggaattcat ccaggaagag tcctgcggaa agtgcgtacc ctgccgcgtg ggaacccgac
    24721 gcatcctgga aattctggaa ggcatttgtg aaggccgcgg caaagagggc gacatcgaca
    24781 ccctgcggtc cttgtgtgcc gaaatcaagg aaaacagcct gtgcggttta ggccagggcg
    24841 cgcccgcacc ggtggtatcc acgctcaacc acttcctgga cgaatatgaa gcccacatca
    24901 aagaaaaacg ctgcccagcc aaagtttgcc gggcgctcat caagtatcaa attatggatg
    24961 gagcctgcac tggctgtaca gtatgcgcgc gcaactgtcc ggtctcagcg ataacaggcg
    25021 aacgcaaaca gactcatgtc atcaatcagg atatttgcat caaatgcggc atctgccatc
    25081 aggtttgcgc ctttgacgcg attacagtaa tttaatgcgc gattagccag tcaagaagga
    25141 gttgaccgat gacagaccag gtaagcgcaa gtatgaaaac gacttcggtc gttgatgaag
    25201 ccattcagca gcacggcgtt tcccgttcgg aattgattcc tatcctcaaa accatcatga
    25261 tgcgcttagg tttcatttca ccagaagttg tcctccaact atcccagaaa ttaaacctgc
    25321 ccagtataga aattacatcg gtagcaacct tttatcgcat gctttccact gcgccgcgcg
    25381 gaaagcacgt catccaattc tgtgaaagcg ctccttgcca cgtcgttggt ggaaaccagg
    25441 tctggcaggc aatcaccaag accctgaacc tccaagcggg ggaaacttcc gcggatcatc
    25501 gttggacgct cattacgacc agctgccttg gagtatgcgg aatcggtccg gtgatgttag
    25561 tggataccga cctctacggg aaccttaccc cggaagaggt gccgcacatt ctcagccgat
    25621 acgactagga gatatccatg aaaacttccc gttcaatggt tttgatctct cacgatccgg
    25681 tcagtatcca gcaaggagcc gacgctgtac tcgaggcatt ccagcaggag ctgaacagac
    25741 tccagcttca ggatgaggtt tcgctttctt tggtggaaga tatcggccgg aattatgcct
    25801 cacccctggt cattgtctac ccggaagcag tcatctacgg gcgcgtgtca gtcgaagacg
    25861 tcccgcacct ggtggaagag cacctgttca aaggtcgcat agtggaagag aaaatcgccc
    25921 ccgcgcacga actt
//
LOCUS       DAOQAD010000017        25890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_1875_length_25890_cov_5.401781, whole genome shotgun sequence.
ACCESSION   DAOQAD010000017 DAOQAD010000000
VERSION     DAOQAD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 25890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25890
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1875_length_25890_cov_5.401781"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2064
                     /locus_tag="PKL60_02705"
     CDS             <1..2064
                     /locus_tag="PKL60_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNW95077.1"
                     /translation="SGWSVVDDTGSLVIDPEGTLTSRNAVEGYWDIYFFGYAQDYLLA
                     IREYQHIAGRPGLIPRWALGNWWSRYWAYTQEELTQLMLDFQERCIPLSVCIVDMDWH
                     ITNTGNNSSGWTGYTWNKELFPDPAAFLAFLHTNKLKTALNLHPAEGVHPHEAQYESM
                     ARAVGIDPATKEPVPFDITSDQFTRAYLEILHHPYEQQGVDFWWIDWQQGTRTKKEGL
                     DPLYALNELHYYDLGRNPEKRPFIFSRWPGLGGHRYPIGFSGDSVVSWESLQFQPEFT
                     ATAANVAFGWWSHDIGGHCDGIEEAELFLRWVQYGVFSPILRIHSTNNPYIDRRPWKL
                     GADAFESIRKSMQLRHALVPLLYSANHRSAEDGEPLVLPVYYEAPSESAAYRCPQEYL
                     FCRQLLAAPFTQKVNPDTRLARQVVWLPEGDWYHFFTGEYVQGGFWYPIYGGKEDIPV
                     FAKAGAIIPLNGDEPGNGVSLPKTMRLKCFPGKDAQFELYEDDGETQQYLEGACAQTI
                     ITQETLPDEICLKIGPALGRFPGMPETRAWILEFENITAPENILIHSEDELIGFESQY
                     DTPAHRLTISLEEIPTAQKIVVQLSGAAPARQSSGVQDHLESFLRAARLPTSVKWMFS
                     KRIADFLEHPKRLLEIWQYFSPAQTLAVLELIFGKQKDPIASDPDTALMDALNAAQKW
                     IASRD"
     gene            complement(2069..2386)
                     /locus_tag="PKL60_02710"
     CDS             complement(2069..2386)
                     /locus_tag="PKL60_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNW95078.1"
                     /translation="MEFFNLGWSEIFLIVLVAFVILGPGKISQAARDFGAWLRKLDKS
                     EAFRDVMHTTDQIRNFPRKIMDEAGLDPVFPDRSMYQEVRKTPGERFPDEKSPPADSG
                     DLP"
     gene            complement(2412..2612)
                     /locus_tag="PKL60_02715"
     CDS             complement(2412..2612)
                     /locus_tag="PKL60_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNW95079.1"
                     /translation="MFGSIGLPEIVLILVVAILIFGVGRISKIGGELGTGIRAFKEGL
                     AGDKANETKETPAENEKTDRSE"
     gene            complement(2614..3351)
                     /gene="tatC"
                     /locus_tag="PKL60_02720"
     CDS             complement(2614..3351)
                     /gene="tatC"
                     /locus_tag="PKL60_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNW95080.1"
                     /translation="MEPQEEGLTIPNHLEEFRSALLRALLGLGAAIVVSLFFTSRLID
                     FLARPIGGLEKLQAIEVTETVSVYMRVALLAGFILASPWIFYQLFGYLAKGLKAEERS
                     RVLVAVPFAVLFFAAGAAFAYIIMLPSALNFFAQFLGVQTLFRIKSYFSFVTDLIFWI
                     GVSFELPLLVFVLARIGIVNASMLIKGWRAAVVVIAVLAAVITPTADPVNMAIFMLPL
                     FGLYLLSIGLAALAGKKRAAAEQEQET"
     gene            complement(3431..3946)
                     /locus_tag="PKL60_02725"
     CDS             complement(3431..3946)
                     /locus_tag="PKL60_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNW95081.1"
                     /translation="MIPIYGICGTSGEGKTRLVSAVLAEASRQRWSTRGVYCPAVFEG
                     NMKTAIAVRLIPGTESRILMRLAQPGDSQVFGQWNLDLESIAWARTYLLDFQPADIWV
                     IDEIGPLEIIYGQGWAEILPYLPLLPARKVLVSFRSSLQPWFLEHYPGITITTAGQPD
                     AEAALLQSLFR"
     gene            complement(3943..5136)
                     /locus_tag="PKL60_02730"
     CDS             complement(3943..5136)
                     /locus_tag="PKL60_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNW95082.1"
                     /translation="MNFLAEAEKFFPETAALRQKLHQYPELAFAEQQTSRLAAEELTK
                     LGFEVTTGLGGTGVSGFLPGKSSMPLIMLRFDMDALPIQEESGAVYASRNPGVMHACG
                     HDAHTAIGITTARLLSRHKAKLDASFMLVFQPAEEIGQGALAMLKDGLFSSRKPDYAL
                     GVHVWNSLPSGSFVINPGELMAGSGVFEIKITGRGGHAALPHLAVDPIAAGARMVSAV
                     QSISAQNVNPLEAAVLGVTMFHAGTASNIIPEQAELQGTIRFFSRQTWELLCERLTRV
                     VQDTASACSCTAELKLLQSTPPLINDPFAARAAQNALKKILPDAALLTDYRTMGSEDM
                     AFFLEQVPGVFIFVGSGGAVEQNAYPHHHPRFDIDESVLPSASALLLQTAAELQHAAA
                     GENPR"
     gene            complement(5140..5865)
                     /locus_tag="PKL60_02735"
     CDS             complement(5140..5865)
                     /locus_tag="PKL60_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNW95083.1"
                     /translation="MNLEQADNWPAGTTILVVLAHPDDPEFFMGGTIARWTAAGYTVK
                     YALLTRGERGISPEFPDSAALASVRVREQKEAAAVLGVHEVTFFDYQDGYLIPSLEIR
                     REVTRLIRSAKADIVVSCDPQNYFIGEAYLNHPDHRAAGQLVVDAVFPAVGNPSFFPE
                     LILDGYPATNVSELWLSLPAQANVLLDVSEFWQQRALALLRHKSQVGDPEKFLAHIEE
                     RRLENSGTDDRYVERFRRIIFRK"
     gene            complement(5862..7004)
                     /gene="ricT"
                     /locus_tag="PKL60_02740"
     CDS             complement(5862..7004)
                     /gene="ricT"
                     /locus_tag="PKL60_02740"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNW95084.1"
                     /translation="MAEQSIIGVQFSPVGKIYHFSVPEGASVLEGSFVLVNTARGRQL
                     GKVLQTSVQNPHNPEDIQPIERVATEQDLQVKERNAEKEKLALEKARDCLKDPHYSAV
                     KAFAAEYSYDGNRLTFYLNYEPDTGFDVRGFLREISHSFRDVRLEVRQLGPRDMAKAI
                     SGLGACGIEKRCCSKFLTEFSSISIKMAKSQDISLTPMEITGMCGRLRCCLNYEYETY
                     EEARKNLPKHKKVVQTPLGEGKVIQVLPLRESVIVELPEIGPRLFTLEELQTGKLDEK
                     KSVPEESIPTFSETKGDVEMLAYGGKSKPESSGKPNRETGNRRDRRGRGGERRGSQQG
                     FHSNTENPGASPQQKGSPKSQQSTNTGPNSQEQSTQGFPERRKKRR"
     gene            complement(7016..8062)
                     /locus_tag="PKL60_02745"
     CDS             complement(7016..8062)
                     /locus_tag="PKL60_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNW95085.1"
                     /translation="MITKDQVLSALSVVIDPDLQKDLVTLGMIEDVEIIDDAVSFTLV
                     LTTPACPLRAQLERDARRAVQVMTGAKEVKIQTKSRVQNAKQVEISTNQFKHIIAVGS
                     GKGGVGKSTIAANLAISLAQDGASVGLLDADIYGPNIPRMLGISGTPQAKEGEKVKPL
                     TAYGVQVVSIGFFVADGQPLIWRGPMLHSAIQQFLTDFEWGCLDYLVVDLPPGTGDVQ
                     LSLSQTLPISGAVVVTTPQAVSIDDAFRAVSMFDKLEIPVLGIIENMAYLRLPDGGQM
                     PVFGEGGGKRLEDLTGVPLLGSIPLEPELGLDGDRGTPAVISSPLNPAAEALRMIARN
                     IAAETSKQAHLAKG"
     gene            complement(8129..10024)
                     /gene="glmS"
                     /locus_tag="PKL60_02750"
     CDS             complement(8129..10024)
                     /gene="glmS"
                     /locus_tag="PKL60_02750"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNW95086.1"
                     /translation="MDSQAQRLSVSEAGRSTKMCGIVAYIGGQNATPIVVNGLKKLEY
                     RGYDSAGVAVLQNDKIEVRRDAGKIARLEALIAEQPLRGCVGIGHTRWATHGAPNQQN
                     AHPHLSSNGEIVIVHNGIVENYLSLRDELLAEGAVFHSDTDTETIVYLIERYYALTSD
                     LAKAVELTMKRVRGAHGIVVMSSREPDRLLAARLGNAGGVAIGKGSGEMFIASDLPAI
                     VEHTREISFLESGQLAIIKRDSLVVQTLDGVRVQPQIHHISWDPVSAEKGNYRHFMQK
                     EIHEQVRSLSDTMIGRIDFDDYRITLPDLNLNAAGAKSIERIFITACGTALHAGMVGK
                     TVIERLTRIPVEVMVASEFRYIDPIVDKNTVVLAISQSGETADTLAAMEEGRRKGAKL
                     WSIVNAIGSQAMRSADGSIPMQVGPEIGVASTKAFTAPLVDLYMLAVFLADLRGSISS
                     EERTKLIRDLLKVPTLAGKTLEHEAEVERSAARFKDMRSVLYLGRGINMPIAYEGALK
                     LKEISYIHAEGYPAGEMKHGPIALVERDLPVLVIAPKDAWYDKMISQIEQAKARGGQV
                     IAVATEGDALIPGLVDEVLWVPEAPWLLSPILTTIPLQLFAYHIATQLGLDVDQPRNL
                     AKSVTVE"
     gene            complement(10551..11174)
                     /locus_tag="PKL60_02755"
     CDS             complement(10551..11174)
                     /locus_tag="PKL60_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNW95087.1"
                     /translation="MADLLHKPKGLSAIAGIILAAGDSSRFGAPKQLQAWGQGSCLEA
                     CIRTAETAGLDPLVLVLGDHYEEIFARTQFGRALVVRNPDWEKGQSTSLKAGLAALPA
                     DCFGAIFLLADQPQVSVNLVGAVKELAWQKDAVILPLINDRRANPVFFPARAFPRLMD
                     LQGDQGGRAILGEFSAVQLPWLDDDMALDIDTREDYDRLSRIFFGKS"
     gene            complement(11180..13360)
                     /locus_tag="PKL60_02760"
     CDS             complement(11180..13360)
                     /locus_tag="PKL60_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNW95088.1"
                     /translation="MDALAKVTGKARYPGDFQFPDQLTMKVLFAHRPHAIIHAIHTEE
                     ALAVPGVVMVLTAKDVPDNEYGLGVRDQPVLCGPGSSKPFAERVRFEGDQVALVIAEN
                     EHAAVQGLKAVRVEYEDLPVVTDAEAAMREDAVRVHPELESNIFHHNIVRLGDVEAAF
                     SRCAVICEREYHTPVQEHAFLQPESGAAYVDKDGVITVIVAGQWTHEDQEQIAHALNL
                     PLEKVRVIYPAIGGAFGGREDMSVQIILALAAQKLHEAGIDRPVRIVWSREESIIGHH
                     KRHYYVLRAKWGADADGKILAAQCVMVSDGGAYVYTSPKVLGNSTLLASGPYDIPNVS
                     TDAYSVYTNNIPAGAFRGFGGPQAAFMAEMQVDALAEALGIDPVEMRMRNLFKEGSIQ
                     SMGQAIPKGVSIREVVRACALEAGWQEVPGGWQKPIIPQGADPNVRTGLGFACGYKNV
                     GFSFGYQENCAAKVVLFGEHEVERARLFHAGAEVGQGAHTVFAQFTAEALGVPMEKIE
                     LCLSDTATSGNSGSVSASRMTFMAGNAILGAARAARERWEAGERPAVGDFTYLAPKTS
                     ALDKKDGHCYPNFAYGYVAEAVLAEVNITTGEVRLRKVICADDVGKAINPSLVEGQIE
                     GAVVQAAGYSLMENFITRDGITRTKELSTYMIPTAVDIPDQVQSVILEIPDPIGPYGA
                     RGMGEMPYLPFAPAVIDAVYQATGVLFNRFPLTAETVLRGLGKF"
     assembly_gap    13416..13425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13449..14855)
                     /locus_tag="PKL60_02765"
     CDS             complement(13449..14855)
                     /locus_tag="PKL60_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNW95089.1"
                     /translation="MWKQYHIVETVEELLSRLASPEGKTKIIAGATDMMVEIRNGKWP
                     ELETVLDISRIQGLDHIFRDREGVVHIGALVTHNDILRSALIREYGFPLLQACAQVAS
                     PQLRNRGTVAGNLITASPANDTITPLMAMEAELVLVSPKNTRVIPLRDFYPGLRKTVM
                     QADEFLSEIRFQGLKADQSGNFKKNALRRAQAISVLNCCAVLTINAGKIQKAALTLGS
                     VAPTVIHAPRAEEFLLGKALDSEVITQAAELAAADAVPISDIRASDAYRNYMLKVLVE
                     GALSEMQDGSSAGSVPARVPTLDSRAGFACKPADNWEGGVIHTTINGQEYFIPDYCDQ
                     TLMSLIRDRVGLTGTKTGCEEGECGACTVFLDGKAVVSCLVPAPRAHNAVITTIEGIA
                     DGTELHPVQQAFIDHAAVQCGYCTPGFVMSAVKLLEEMPGPDRESIQRGISGNLCRCT
                     GYYKIIEAIESASLRMEA"
     gene            complement(14866..15654)
                     /locus_tag="PKL60_02770"
     CDS             complement(14866..15654)
                     /locus_tag="PKL60_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HNW95090.1"
                     /translation="MDVILQKLAEYNQQQKAVCLCVVVSAQGGVPRHAGSKMLVFPDG
                     SSLGTVGGGEVEAAAKAAALEALRTQKPALVSYSLNARDEGALGICGGAMQVYVEPFL
                     ARPKLLIIGAGHVGKAVAKLGKFLNFTVVVSDDRAELCTSAEFPEADLLLPMPMAEIP
                     QQLEIDSQTYIVDVSRGSELDTAGLPALLAAQPAYIGVIGSRRRWEATRAGLLENGVR
                     EAEIARIKSPVGLNIHAETPEEIAVSILAEIISLRNTAAERTKE"
     gene            complement(15654..17030)
                     /locus_tag="PKL60_02775"
     CDS             complement(15654..17030)
                     /locus_tag="PKL60_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNW95091.1"
                     /translation="MMQTFGNTQKFLERVFVTGHINPDTDSIAAAVGYAWLLSERDGV
                     QSVAARAGAINRQTAFVLKLLELDAPLLLTDASPRFESVMRRLDVVSPDKSLLEAWTI
                     LTRTGGIAPVVNTDGTPFGIVTGKSLFDFLRKIVGPHPKYRDMTIADLLDIPCREAAQ
                     SDIPKFQPGTRIKDVINRLLRLDADEYWVLDDHKRYLGVVRQRDLLNPPRIKVVLVDH
                     NEPQQSIASLEEAELLEILDHHRLGNQSTHQPIKFTVDTVGSTSTLVSEQTEDAGLSA
                     PPAVAGLLLAGLLSDTLILTSPTTTERDRAAAERLARWAFVPGSALEAETIKSYGEKI
                     VQAGAGLATQVPDEVVTTDMKVYETAGYKFAIAQAETSDIYEVGDYIPKLQSALKNLK
                     NAQGLDFAGLMVTDVVEGSSRLIFVDAPPALDELPYRPLPDGTRFAEGVVSRKKQLLP
                     IVLSLLEV"
     gene            complement(17042..19939)
                     /locus_tag="PKL60_02780"
     CDS             complement(17042..19939)
                     /locus_tag="PKL60_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNW95092.1"
                     /translation="MNFDLHINGQSVAVEAAPDEKLLDVLRRLGYLGVKSGGCKRGEC
                     GACTVLMDGKAVNSCMFLAVQALGHEIVTIESLGTPPNQGWKETEGLSPLQKAFIQNG
                     SIQCGYCSSAMILAAKALLERNPDPSEAEVRGALSGILCRCTAYVKPVEAVLQAAAMM
                     RAQQNRAAVSEPKIVPVDAIPSTQVIGKSTVKVDAEKLAQGRQAFTADFARPGTLVAR
                     VLRSPLAHARIVRLNTDKAKALKGVAAVLTWEDLPRVVYSTAGQSDPIPGPLDSFSLD
                     NKVRFVGDRVAFVAAESAEIAEQALALIEVDYEELPPVLDPRDAMITGAPIIHDEGEY
                     VNFADSDPGINLAARIRIDIGDVEKGFAEADRVFEREYVVPKVQQAHIEPHVALSYWD
                     PDNRLVIVTSTQVPFHARRMLAPLLKLPVKRIRVIKPRIGGGFGNKQEVLVEDVAAHL
                     TIATGKPVIYEMSREEEFIASRSRHPMRIKMRTGVKLDGTMTANEMIALSDTGAYGCH
                     ALTVTGNTGQKSMALYVGDEEYRKSPNIRFYADIVYTNTPPAGAYRGYGVPQGYWPVD
                     RHMEMIARELGLDPLAFRLKNALRSGEFQPFSTSWNEGREPRPEIIHTVGLEACVREG
                     AEAIGWQEKYGNSSWHEIPGKPHLRRGIGAALVMQGTAIPYLDMGGASLKLNDDGSFN
                     LLVGATDLGTGSDTVLAQMAAEVLGVNADDIIVYSSDTDFTPFDKGAYASSTTYISGT
                     AVIKAAAQVAQRIKARASEMFADAGTQAAPESITLADGKAQEPGGKCYTLAEIALNSL
                     HHTNQEQIMAVASHVSPSSPPPFAAQFASVLVDLQTGQVTVEDLVMAVDGGVIINPAA
                     ASGQIEGGMAQALGYGVCEEMVYDGRGRAREKDLVDYHIFRADEMPRMQTIFVETYEK
                     SHPFGAKAVAEIPMDGVAPAVGNAVMDACGADIVENPITPEKVWRARTLIV"
     gene            complement(19936..20721)
                     /locus_tag="PKL60_02785"
     CDS             complement(19936..20721)
                     /locus_tag="PKL60_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNW95093.1"
                     /translation="MRNDENRLKRPKNLEELAVLFRETHGCLRILAGGAYVPSRLGSS
                     DLFVDIQDLGADRVEKSGDILRIGAAASLQQILEADTGLEELRTPLELEAGANARSSL
                     SLLNFLRSADQRSPFLTALLALAPELELAPSGRFVSLERFPRSQPENEREFPLWMSFR
                     IPDDFAWEFIARTPKDRPIISLAAARKKDSALRIACGGDEHPPRVISQSAKAEALFAL
                     VRHAYEASGDAWASAEYRQEMSQVLLSRCLQRLESNALKKEEL"
     gene            complement(20711..21175)
                     /locus_tag="PKL60_02790"
     CDS             complement(20711..21175)
                     /locus_tag="PKL60_02790"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNW95094.1"
                     /translation="MKKQATSDTCFVCGRKNPVGLDLSFFVDENGDTIAYVQFGDQYQ
                     GYPGIVHGGVTAAVLDEICGRAFSDHGDQFMVTSELTLRYRKPVPLNTPLIAKGYKVK
                     REGRVAFARGELLDQTGAVLVEASGIYVDMPEEKLQEMNPDSFGWRVIPDEK"
     gene            complement(21172..22176)
                     /locus_tag="PKL60_02795"
     CDS             complement(21172..22176)
                     /locus_tag="PKL60_02795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNW95095.1"
                     /translation="MTKRVAIYLQDAHDLREGLDIVRYAEAKGFEAVWQAESRLVRDA
                     IVPMAAYAAVTERIKVGSGVINNWTRNIGLLASTFLTLDDLAPNRIICGIGAWWDPLA
                     KNVGINRTKPLKAMEETITVMRKLLNMERVTFEGEFVNVHGIELDVVHGRREPRHVPI
                     YIGATGDKMMEMAGRIADGVVMNYCVPVNYNDIAIEQLMVGLKIAGRRLEDFDRPQLV
                     VCSVDEDHDRAIDTTRELLCQYLAQQPHIAKASGVSTEVVQKIQAILGWPATHEQIQQ
                     AKHLVPEDLIHRITASGTPSEAKAKVQEYIDHGCTCPILYAVGGNARLLIDTFAPEQA
                     "
     gene            complement(22214..22813)
                     /gene="cofC"
                     /locus_tag="PKL60_02800"
     CDS             complement(22214..22813)
                     /gene="cofC"
                     /locus_tag="PKL60_02800"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNW95096.1"
                     /translation="MITWALIPVKRFDLGKSRLHEVLSARELAAVNQKLFRRTLSTAK
                     ESGIFRHMMVISEDEGALAWALEQGFFALREEPSGSLNSALRQALQTIQADSAGSVLI
                     IPTDLPLLIAEDLRLLSGFTPAENGMLLVPDYRMTGTNALFLSRSDLMPTAFGNNSCA
                     LHCALAEERRLQHVIYLNTHIQHDLDTPEDLLYLRESLI"
     gene            complement(22810..23787)
                     /gene="cofD"
                     /locus_tag="PKL60_02805"
     CDS             complement(22810..23787)
                     /gene="cofD"
                     /locus_tag="PKL60_02805"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HNW95097.1"
                     /translation="MHPDFTEFSALKVAALAGGVGGAKLARGLDKVLPAGNLTVIVNT
                     GDDFEHFGLRISPDLDSVCYALAGLADPVKGWGRANETWNSFSALVELGAPAWFQLGD
                     KDLALHLERTRLLQAGWDLSAVTCHFCERWGVRSRVLPMTDALFRTRVRTSDGSLLDF
                     QEYFVGEKCAPAVSGFVFSHAEDVQPAPGVLTALEEADLVILCPSNPWVSIDPILALP
                     GIRQSLREKTVLAVSPIIQGKTVKGPAAKMFAELGIEPGSPSVLAHYEDFLDGFVFDQ
                     LDSGLFQNYEGERIILLGTHTLMQNDEDKTRLAAEVLRFGVDLIVRKAP"
     gene            complement(23787..24566)
                     /gene="cofE"
                     /locus_tag="PKL60_02810"
     CDS             complement(23787..24566)
                     /gene="cofE"
                     /locus_tag="PKL60_02810"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNW95098.1"
                     /translation="MLQPKSLILCTVPDLPEIHPGDDLPELIIGALTQHAMALEDGDI
                     LVVAQKIVSKSEGRLVNLSTVTPGPRALELAAQIGKRPELVQLILDESSAVLRMRPGT
                     IIVEHKSGFVCANAGIDHSNVRGPWGAPEDWVLLLPEDADYSARVLREGLVRLSGKRV
                     GVLIIDSHGRAWRNGIVGISIGLAGMPALVDMRGKPDRFDYALRVTVIAAADELAAGA
                     SLMMGQAAEGTPVVLARGFPYELADDQAASLVRDKKYDLFR"
     gene            complement(24568..25227)
                     /gene="npdG"
                     /locus_tag="PKL60_02815"
     CDS             complement(24568..25227)
                     /gene="npdG"
                     /locus_tag="PKL60_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNW95099.1"
                     /translation="MAYTIGIIGGTGKEGKGLAYRWAKAGHKVLIGSRLEEKARTAAA
                     ELKALLPAEARVEGFSNAAAVEQCDIAVLTVPFTAHAETLQGLKDALQGKILIDVVVP
                     LNPAKATRVSIPPEGSAAQQAQKILGEGVQVVAAFQNIAYEHLLNDESVACDVLVAGT
                     GKEARRTVIGLVQDAGLRGWDAGVIENSVVIEGLTPILIGLNIQHKVSSAGIKITGIP
                     E"
     gene            complement(25241..>25890)
                     /locus_tag="PKL60_02820"
     CDS             complement(25241..>25890)
                     /locus_tag="PKL60_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNW95100.1"
                     /translation="DQENSLRKYGKRVIERFYEQGILGEKSILAHGVHTNSREFDLLA
                     ETGTWLSHQPRSNMNNAVGVTPVLDMLKHEVNVVLGNDGFSNAMWEEWKAAYFIQKDH
                     GHDPRLMNGYDVMKIAVENNSRLATQTFDGLRVGIIEPGAAADLILVDYHPITPLSAG
                     NLPWHILFGFREGMVTSTIAAGKLLMRDRKLLGLDEAEIAAKAREAALRTWKKIQ"
BASE COUNT         5377 a         7813 c         6975 g         5715 t
ORIGIN      
        1 tccggctgga gcgtcgtcga cgatacgggt tccctggtaa tagacccgga aggcacgctg
       61 acgagcagaa acgcggtgga ggggtactgg gacatttact tttttggcta cgcgcaggat
      121 taccttttag caatccgcga ataccagcat atcgccggcc gacccgggct tatcccgcgt
      181 tgggcgttgg gcaactggtg gagccggtac tgggcataca cccaggagga actgacgcag
      241 cttatgctgg atttccagga gcgctgcatt cctttgtcag tttgcattgt ggatatggat
      301 tggcatatca ccaacaccgg aaataattcc agcggttgga cgggttacac ctggaacaag
      361 gagctttttc cggatcccgc ggcttttttg gcgtttctcc acacaaacaa gctcaaaacc
      421 gcactgaatc tgcaccccgc ggaaggcgtg cacccgcacg aagcgcagta tgaaagcatg
      481 gcacgcgcgg tcggcataga cccggcaacg aaagaacctg tgcctttcga cataacgtcc
      541 gaccaattca cgcgcgcgta tctggaaatc ctgcaccatc cttacgagca gcagggcgtc
      601 gacttttggt ggatagactg gcagcagggt accagaacca aaaaagaagg cttggatccg
      661 ctttacgctc tgaacgaact gcattactac gatctcggca gaaaccccga aaaacgcccc
      721 tttattttct ctcgctggcc cggcttgggc gggcaccgct acccaatcgg cttttccggt
      781 gattcggtcg tctcctggga atccctgcag tttcagccgg aattcactgc cactgccgcg
      841 aatgtggctt tcggctggtg gagccatgac atcggcgggc actgcgacgg catagaggaa
      901 gcggagttat tcctgcgctg ggtgcaatac ggcgtcttca gcccgatttt gcgcatccat
      961 tccacgaaca acccctacat tgaccgccga ccgtggaagc ttggcgccga tgccttcgag
     1021 agcattcgga aatcgatgca gctgcggcac gccttggtcc ccctgctcta ttccgctaac
     1081 caccgcagcg cggaagacgg cgaaccgctg gtgcttccgg tttactacga agccccgtct
     1141 gaatctgccg cttaccgctg cccgcaggaa taccttttct gccgccagct tctggcagcg
     1201 cccttcaccc agaaggtcaa tccggacaca cgccttgcca ggcaggtcgt ctggctgccg
     1261 gaaggcgatt ggtaccattt ctttacgggc gaatatgttc agggcggctt ttggtatccc
     1321 atctacgggg gcaaggagga catcccggtc tttgccaaag ctggcgcgat tatcccgctc
     1381 aacggggatg aaccggggaa cggcgtgagc ctgccaaaga cgatgcgcct caagtgcttc
     1441 cctggcaaag acgcgcagtt cgaactctat gaagacgacg gagaaactca gcagtatttg
     1501 gagggcgcct gcgcgcagac tatcatcaca caggaaacgc tgccggatga gatttgcctg
     1561 aaaatcggcc cggcgcttgg caggttcccc gggatgcctg agacccgcgc ctggatcctt
     1621 gaatttgaaa acatcactgc gcctgagaat atcttgattc acagcgaaga cgaacttatc
     1681 gggtttgaat cccaatatga tacgcccgct caccggttaa caatttctct ggaagaaatt
     1741 cccacagccc aaaaaatcgt tgttcagctt agcggcgctg cccccgcgag acagagctct
     1801 ggcgtgcagg accacctgga aagcttcctc agggctgcgc gccttcccac ttcggtcaaa
     1861 tggatgttca gcaagcgcat tgccgacttc cttgagcacc ccaaacgttt gctggaaatc
     1921 tggcaatact tctcccctgc gcagacgctt gccgtgctcg agttaatttt tgggaaacaa
     1981 aaggacccga tagcttcgga tccggataca gcgcttatgg acgccctgaa cgccgctcaa
     2041 aaatggatag cctccaggga ttaatgcttt agggcaggtc gccgctatct gctggcgggg
     2101 atttctcatc cggaaaacgc tcgccgggag ttttccgcac ttcctggtac atggaccgat
     2161 ccggaaagac cggatcgagc ccggcttcgt ccatgatttt ccgcggaaaa ttacggattt
     2221 ggtccgtggt atgcatgacg tcccggaaag cttccgattt atccagtttt cgcagccatg
     2281 cgccaaaatc gcgggctgcc tgagaaattt tccctggtcc gaggatgaca aatgccacca
     2341 atacaatcag gaatatctcg gaccatccaa gattaaagaa ttccatgcta aactaccgtt
     2401 tcgctatgcg cttactctga acggtctgtt ttctcgtttt cagcaggcgt ttccttcgtc
     2461 tcattggcct tatcgccagc caggccctcc ttgaaggcgc ggatgcccgt gcccagttcc
     2521 cctccaatct ttgaaattcg gccgacgcca aaaatcagaa tcgccacaac caaaatcaaa
     2581 acaatttccg gtaaaccaat cgatccaaac actttatgtc tcctgttcct gctcagccgc
     2641 cgcccgtttc ttcccggcaa gcgcggccaa accaatactc agtaaatata acccaaaaag
     2701 cggcagcata aaaatggcca tgttaacagg gtccgcggtg ggcgtgataa ccgccgcgag
     2761 caccgcgata accacaaccg ccgcgcgcca gccttttatc agcatggatg cgttcacaat
     2821 gcctatcctc gcaagcacaa ataccaataa gggcagttca aaactgacac caatccagaa
     2881 tatgaggtcg gtaacaaagc taaaatagga cttaattcgg aaaagagtct gcactcccag
     2941 gaattgcgcg aagaagttga gcgctgatgg cagcataatg atataagcaa atgcggcgcc
     3001 tgccgcgaaa aagagcaccg caaaggggac tgcgacaagc acccggctgc gctcctccgc
     3061 tttcaagccc ttggccaggt acccgaacaa ctggtaaaaa atccaggggg aggccaggat
     3121 aaagccagcc aacagcgcga cccgcatata aacggaaacg gtctccgtta cctcaattgc
     3181 ctgcagcttt tccaggccgc caataggacg ggcgaggaag tcaatcagcc ggctcgtgaa
     3241 aaaaaggctg acgacgattg ctgcgcccaa accaagcaaa gcacgcagca gcgcgctccg
     3301 gaattcttcc aggtgattgg gaatagtaag gccttcttct tgtggttcca cgtttcaaat
     3361 tatacagcgc atggaaacgc tgccccttac aggcctgtac gccgcgcgtc gccaggcatg
     3421 ctgatagaac ctacctgaac agagattgaa gcagcgcagc ctcggcatcg ggctgcccgg
     3481 ctgtggtaat agttatgccg ggatagtgct ccaggaacca cggctgcagg cttgaacgga
     3541 aacttaccag caccttcctg gcgggcagta agggaaggta gggcagaatc tccgcccacc
     3601 cttgaccgta gatgatttcc agcggaccga tttcatcgat aacccaaatg tccgcgggct
     3661 gaaaatccag cagatatgtc cgcgcccagg cgatgctttc caaatccaga ttccactgac
     3721 caaacacctg ggaatcgcca ggctgtgcca gccgcatcaa aattctggat tccgtcccgg
     3781 gaatcaaacg caccgcaatt gcggttttca tattaccttc aaaaacagcc gggcaataaa
     3841 ctccccgcgt gctccagcgc tgccggctgg cctcagcgag aacggcagaa accagacgcg
     3901 ttttcccctc cccgcttgta ccgcagatgc cgtagatcgg gatcaccgcg ggttttcccc
     3961 ggcggcagca tgctgaagtt cagcagcagt ctgcagcagt aaagcgctgg cgcttggcag
     4021 tacgctttcg tcaatgtcaa agcggggatg atggtgcgga taagcattct gctccacggc
     4081 gccgcccgag cccacaaaga taaacactcc cggaacctgt tcgaggaaaa aagccatatc
     4141 ttctgaaccc attgtgcggt aatccgtcaa aagagcggca tccggcagga ttttcttgag
     4201 cgcgttctgc gcggcacgcg ctgcgaacgg gtcgttgatc agcggcggcg tactttgcag
     4261 cagctttagc tcagccgtac agctgcaggc gctggcagta tcctgcacga cgcgggtcag
     4321 gcgctcacac agcaattccc acgtctgccg gctgaaaaag cggattgtcc cttgcagctc
     4381 agcttgctcc gggatgatgt tgctggctgt acctgcgtgg aacatcgtga cgcccaaaac
     4441 cgcggcttcc agcgggttca cattctgcgc gctgatggat tgcacggcgc taaccatccg
     4501 cgctccagca gcgattgggt cgactgccag gtgcggcagg gcagcgtgcc cgcctctccc
     4561 ggttatcttg atttcgaaca cgccgctgcc cgccatgagt tcgcctggat ttattacgaa
     4621 actgccgctt ggcaggctgt tccagacgtg cacacccagc gcataatcag gcttccggct
     4681 gctgaataag ccgtctttca gcatagccag cgcgccctgc ccgatttcct cagccggctg
     4741 aaataccagc atgaacgatg cgtccaactt tgctttgtgg cgggagagca gccgtgcggt
     4801 ggtgatgcca atcgcggtgt gcgcgtcgtg cccgcaggcg tgcatgaccc cggggttccg
     4861 tgaggcgtac accgccccgc tttcctcctg gatgggcagc gcgtccatat caaagcgcag
     4921 cataatcaac ggcatagacg atttcccggg aaggaagccg ctcacgcccg tcccaccgag
     4981 cccggtcgtg acctcaaagc cgagcttggt cagctcttca gccgccagcc tggaggtttg
     5041 ttgttcagca aaggctaatt cgggatattg gtgcagtttt tgccgcagcg cggcagtttc
     5101 ggggaagaat ttttcagctt ctgccaggaa gttcattttt cacttccgga aaataatccg
     5161 gcgaaagcgt tcgacatagc ggtcgtcagt gccgctgttt tccagccggc gctcttcgat
     5221 atgggcaagg aatttttcgg ggtccccgac ctggctttta tgccggagca aagccagcgc
     5281 gcgctgctgc cagaattcgc tcacatccag gaggacgttc gcctgcgccg gcaaactcag
     5341 ccacagctcg gaaacattcg tggcagggta cccgtccaga atgagctcag gaaaaaagct
     5401 tggattgccc acagccggga acacagcatc taccacaagc tgaccagctg cgcggtggtc
     5461 gggatggttg agataagctt ccccgataaa gtagttctgc gggtcgcagg aaacgacgat
     5521 atccgctttg gcgctgcgaa tcaatcgcgt cacctcgcgc cggatttcca ggctagggat
     5581 gagatacccg tcctggtaat cgaagaatgt gacctcgtgc acacccagga cagctgcggc
     5641 ttctttttgc tccctcacgc gcacactcgc aagcgctgcg ctgtccggaa attccgggct
     5701 aattccgcgc tcccctctcg tcagcaaagc gtactttacg gtgtagccag ccgcggtcca
     5761 acgcgcgatg gttccgccca taaagaactc gggatcatcc ggatgcgcaa ggaccacgag
     5821 gatagtcgtg cctgcgggcc agttatctgc ttgctccaag ttcatctgcg cttttttctc
     5881 ctctcaggaa agccttgggt tgattgctcc tgggaattgg ggcccgtatt cgtgctttgc
     5941 tgggatttcg ggcttccctt ttgctgggga gaggcgccgg gattctctgt gtttgaatgg
     6001 aatccctgct ggctgccccg gcgctcgccg ccgcggcctc ttctgtcgcg tctgttcccg
     6061 gtttcgcggt tcggtttgcc gctgctttcg ggtttggatt tcccaccata agcaagcatc
     6121 tccacgtcac ctttggtttc actaaaggtc ggaatactct cttcaggcac gctctttttt
     6181 tcgtccagct ttcccgtctg caattcttcc aacgtgaaca gacgcggccc aatttccggt
     6241 agttccacaa ttacagattc tctgagggga agcacttgaa ttaccttgcc ttcacccagc
     6301 ggggtctgca ccactttctt gtgcttgggc aggttcttgc gcgcttcttc ataggtttcg
     6361 tattcataat tcaggcagca gcgcaggcgc ccgcacattc cggtaatttc cataggagtg
     6421 agcgaaatat cctgcgactt tgccatcttg atggatatag aactgaattc ggtcaggaac
     6481 ttggagcagc agcgtttctc aataccgcag gcgcccagac cagaaatcgc tttcgccata
     6541 tcccgcggac caagctgccg tacttccagg cgcacatcac ggaaggagtg ggaaatttcc
     6601 ctcaggaaac cgcggacatc gaagccggtg tcaggttcat aattgagata gaaagtgagc
     6661 cgatttccat cgtacgaata ttccgcagcg aaagccttca cagctgagta gtgcggatct
     6721 ttcagacaat ccctggcttt ttccagggca agtttctctt tctccgcgtt tctctctttg
     6781 acctggaggt cctgttctgt tgccaccctt tcaatcggtt gaatgtcttc agggttatgt
     6841 gggttttgga cggaagtctg aagcaccttg ccaagctgtc tgccgcgggc tgtgtttacc
     6901 agcacaaagc tcccttccag gacgctggcg ccttcgggta ccgagaaatg gtagattttc
     6961 ccgaccggag aaaattgaac gccaatgatg gactgttctg ccatggtgtg ttgttttatc
     7021 cttttgccag gtgggcttgt ttgctggtct cagccgcgat attcctggca atcattcgga
     7081 gcgcttcggc tgcagggtta agcgggctgc taatgactgc cggggtaccg cgatcgccat
     7141 ccaggccgag ctcgggttcg agtggaatgc tacccaacag gggtactccg gtcaggtctt
     7201 ccagccgctt gccgccgcct tcgccaaaaa ctggcatctg accaccgtcg ggaagcctca
     7261 ggtatgccat gttctcgata attccgagga cagggatttc gagtttatcg aacatggaaa
     7321 ccgcgcgaaa tgcgtcgtca atcgagacgg cttgcggcgt ggtaacgacc accgccccgc
     7381 tgatgggcaa agtctgcgag agcgagagtt gaacgtcgcc cgtgccaggc ggaaggtcaa
     7441 ccacaagata atcaaggcag ccccattcaa aatcggtcag aaactgctga atggcggaat
     7501 gcagcatggg accccgccaa atcaacggct gtccgtccgc gacaaagaag ccaatcgata
     7561 ctacctgtac gccgtaagcg gtcagcggtt ttaccttctc gccctctttg gcttgcggcg
     7621 ttccgctaat tccgagcatg cggggaatgt ttggcccata aatgtcggca tccaagaggc
     7681 cgaccgaagc cccgtcctga gccaggctga tcgccaggtt cgcggctatc gtacttttcc
     7741 caacgccgcc cttcccggag ccaaccgcga tgatatgctt gaattggttg gtcgaaattt
     7801 caacctgttt ggcattctgc acgcgggatt tggtctgaat tttcacttcc tttgccccgg
     7861 tcatcacctg tacagcccgg cgggcatcgc gctccaattg ggctctcagc gggcaagcgg
     7921 gtgtggtcaa caccaaggta aaactcaccg catcatcgat gatttccacg tcttcgatca
     7981 ttcccagggt gacgaggtcc ttctgcaggt ccggatcaat caccacagat aaagcactca
     8041 atacctgatc tttcgtaatc aaagtgttct cctgtatttt tttattagtt tactgcaatt
     8101 cccgtcagca ctgcaaaaac gtaagacctt actccacggt gacggatttt gccaggttgc
     8161 gcggctggtc cacgtccaga cctaactgtg tggcaatatg ataagcaaac aactgcagcg
     8221 gaatcgtagt aaggattggg ctcaagagcc agggcgcctc tggcacccac aacacttcgt
     8281 ccaccagacc ggggatcagc gcgtcgcctt ccgtggcaac ggcaatcacc tgcccgccgc
     8341 gggcttttgc ctgctcgatt tgactgatca ttttgtcata ccaggcatcc tttggcgcga
     8401 tgaccaacac aggcaggtcc ctctccacca gcgcgatggg tccgtgcttc atttcgcccg
     8461 ccggatagcc ttccgcatgg atatacgaaa tttcctttaa ttttaaagcc ccttcatacg
     8521 cgatgggcat attaattccg cgccccagat acagcacgct gcgcatgtcc ttaaagcgtg
     8581 ccgcagatct ctcgacttct gcttcgtgct caagcgtttt ccctgccagc gtgggcacct
     8641 tgagtaaatc ccggatcagc tttgtgcgct cctcggagct gatgcttccg cgcaggtcgg
     8701 ccagaaaaac cgccagcatg tacaggtcca ccagcggcgc ggtaaaagct ttggtggatg
     8761 ccacaccaat ttcaggcccc acctgcatgg gaatcgaacc gtcagcgctg cgcatggctt
     8821 gcgaaccgat ggcatttaca atgctccaca gcttggcgcc tttgcgtcgg ccttcttcca
     8881 tggcagccag ggtatccgct gtttcaccgg actggctgat agccagcacg accgtatttt
     8941 tatccacgat tgggtcgatg taacggaatt cggaggccac catcacctcc accggaatgc
     9001 gggtaaggcg ttcgatgaca gtcttgccaa ccatgccggc atgcagcgcg gttccgcaag
     9061 ccgtaatgaa aatgcgttcg atgcttttcg ctcccgccgc gttcaggttc aggtcaggca
     9121 gcgtaatgcg gtaatcgtcg aaatcgatac gcccgatcat ggtgtcggaa agcgagcgca
     9181 cctgttcgtg aatttctttc tgcataaagt gacggtaatt ccctttttcg gccgataccg
     9241 gatcccagga gatgtgatgg atttgcggct ggactctcac cccatccaga gtttggacga
     9301 ccaggctgtc gcgcttgatg attgccagct ggccgctttc gaggaagctg atttcccggg
     9361 tgtgttcaac gatggcaggc aggtcgctgg cgatgaacat ctcgccgctg cctttaccaa
     9421 tggctacccc gccggcatta cccagacggg ccgccaacag ccggtctggt tcccgggagg
     9481 acatgaccac aatgccgtgc gcgcctctga cgcgtttcat cgtcagttcc acagctttgg
     9541 caaggtcgct ggtgagcgcg taatagcgtt caatcagata gacgatcgtt tcggtatcgg
     9601 tatcagagtg aaaaaccgcg ccctcagcca acagctcatc gcgcaagctg agataattct
     9661 ccacaatacc gttatggacg atgacaatct cgccattcga gctcaggtgg ggatgagcat
     9721 tttgctggtt cggcgcgccg tgtgttgccc agcgcgtatg cccgatgccg acgcagcccc
     9781 gcaaaggttg ctctgcaatt aaggcttcca ggcgggcaat tttgccggca tcgcgccgca
     9841 cttcgatctt atcattctgc agcacagcca cgcccgcgga atcatatccg cggtattcaa
     9901 gtttctttaa tccattgacc acaatcggcg tggcgttctg ccctccgatg taagccacaa
     9961 ttccgcacat cttagtactc cttccagctt cggagacgct caggcgctgg gcttgagagt
    10021 ccatctatgt tccggacaca ggaaatcgga tggtgtgtca tccggggggc atccgccgat
    10081 ctttcgattt tcccaatgaa aattggttaa ccttccctcg cggaaaggaa cccccgtcct
    10141 cgtcacctgg cttttgccag gcctggcgct ggtttccggt gctgcgtgga ttatacaata
    10201 aaacaaattt cagagttcga gggtgtagaa aaacaacaat ttgactttca ggatgatttc
    10261 gttttggtag ccttattctc aagaatgcgc cacagggttc cctgaccccc accttatacc
    10321 ccggcggcaa gcggatttta tagcgcgatt agtccgctgg agaagggaaa aacgttcggt
    10381 agggggcgct gtccacgatc aaatatcacc tccccgtccc agtgatagtt catcaggatc
    10441 gtaggattaa ttgattccgc tgggaaagaa agcaagaggg gaaaggctgt tcgtacgcat
    10501 gttcgtcaat aatgttttga aagttctatc tagaacctta atcatcaccc tcaggatttc
    10561 ccgaagaata tgcgcgaaag tctgtcatag tcctcccggg tatcgatatc cagcgccatg
    10621 tcgtcatcca gccagggcag ctgcaccgca gagaattctc caaggatggc tctaccgccc
    10681 tggtcaccct gcaggtccat caggcgcggg aaagccctgg ccgggaaaaa taccggattc
    10741 gcccggcggt cattgatcag cggaaggatc accgcatctt tctgccaggc tagttccttc
    10801 accgcgccaa cgagattgac cgatacttgc ggctggtcag ccagcagaaa aattgcacca
    10861 aaacagtctg ctggcagagc ggcaagcccg gctttcaagg aggtgctttg tcccttctcc
    10921 cagtccgggt tccggacaac cagcgcgcgg ccgaactgcg tgcgcgcaaa gatttcttca
    10981 tagtggtcgc ccaatacaag cacaagggga tcaagcccgg ctgtttccgc ggtccggatg
    11041 caagcttcca ggcacgagcc ctgcccccag gcttgcagct gtttaggcgc gccaaaacgg
    11101 ctcgaatcgc cagcagccag gataattccg gcaattgccg aaagtccctt gggtttgtgc
    11161 aacaaatctg ccacggttgt tagaattttc ccaatccgcg caaaactgtt tccgcggtca
    11221 gcggaaaacg attgaacagc actccggtcg cctggtagac cgcgtcgata acggccgggg
    11281 caaagggcag gtagggcatc tcacccatgc cgcgcgcgcc gtatgggccg atagggtccg
    11341 gaatttccaa gatgacgctt tgtacctggt cggggatgtc cacagccgtc gggatcatat
    11401 acgttgacag ctctttcgtg cgcgtgatac cgtcccgggt gatgaagttt tccatcaggc
    11461 tgtatccggc tgcctgaacc accgcgcctt caatctgccc ttcaaccaac gagggattta
    11521 tcgctttgcc aacatcatcc gcgcagataa ccttgcgcag acgcacttcg ccggttgtga
    11581 tgtttacctc cgccagcacc gcttcggcca catatccgta cgcgaagttc ggatagcagt
    11641 ggccatcttt tttatccagc gcgctcgttt ttggcgcgag gtaagtgaaa tcccctaccg
    11701 cagggcgttc gccagcttcc cagcgttccc gagcagctct tgccgcgcca aggatggcgt
    11761 tgccagccat gaaggtcatg cgggaagcgg atacgctgcc cgaatttccc gatgtcgcgg
    11821 tgtcagacag gcacaattca atcttctcca tcggcacgcc cagggcttct gcggtgaact
    11881 gcgcgaacac ggtgtgcgcc ccctgcccga cttcagcgcc ggcatggaag agcctggctc
    11941 tttcaacttc atgctcgccg aacaggacga cctttgccgc gcaattttcc tgatatccaa
    12001 acgaaaagcc tacattttta taaccgcagg caaaaccgag tccggttctg acgtttgggt
    12061 ctgccccctg cggaattatc ggtttttgcc atccgcctgg tacttcctgc cagccggctt
    12121 ccagcgcaca cgcccgcacg acttcacgga tgctcacacc cttggggatg gcttgcccca
    12181 tggactggat ggaaccctcc ttgaataggt tgcgcatgcg catttccaca gggtcgatac
    12241 ccagcgcttc cgccagggcg tccacctgca tttcagccat aaaggccgcc tgcgggccgc
    12301 cgaaaccgcg gaaagcgccc gcgggaatgt tgttggtata cacggaatac gcgtctgtac
    12361 tgacattggg gatatcatag gggccgctgg ccagtaaggt gctgttcccc agaaccttcg
    12421 gggaggtata cacgtacgcc ccgccgtcgc taaccatcac gcattgcgcc gccaggattt
    12481 tcccgtccgc gtccgctccc catttggctc tcagcacgta atagtggcgc ttgtgatgcc
    12541 cgatgatgga ttcctcgcgg ctccagacta tccggaccgg ccggtcaatg ccagcctcgt
    12601 gcagcttctg ggctgccagc gccagaataa tctgcacgga catatcctca cgcccgccaa
    12661 acgcaccgcc aatcgcagga tagatgacgc gcactttctc caggggaaga ttcagcgcgt
    12721 gcgcgatttg ttcctggtct tcatgtgtcc actgcccggc gacgattacg gttatcacgc
    12781 cgtccttgtc cacgtaagcg gcgccggatt ccggctgcag gaaggcatgt tcctggacag
    12841 gcgtgtggta ttcgcgttcg cagattaccg cgcagcgcga aaaagcagcc tccacatctc
    12901 ccaagcgtac gatgttgtga tggaaaatat tgctttcgag ctccggatga acccggaccg
    12961 catcttcccg catagccgct tccgcatccg tcactaccgg caaatcctca tactcaaccc
    13021 ggacggcctt caagccctga accgccgcgt gttcgttttc cgcgataacc aatgccacct
    13081 ggtcgccctc aaagcgcacg cgctccgcga agggtttgga cgagcccgga ccgcagagca
    13141 ccggctggtc cctcacgccc agcccatatt cgttatccgg cacatctttg gcagtaagga
    13201 ccatgacaac cccgggtaca gccagagctt cctccgtgtg gatggcatgg atgatggcgt
    13261 gcggccggtg cgcaaagagc actttcatgg tgagctggtc gggaaattgg aaatccccgg
    13321 gatatcgcgc ttttccggtt acttttgcca gcgcatccac gcgcgggagc gatgtgccta
    13381 cggtctgaaa cccaggtacc tgctctcgac cgctannnnn nnnnngcatg ggcaaatcct
    13441 gctccatctt acgcctccat cctcaggctg gcggattcaa tcgcctcgat tatcttgtaa
    13501 taaccggtgc agcggcacag gtttccggaa atgccgcgtt ggatactctc gcggtccggc
    13561 cctggcatct cctccagcaa tttcacagcc gacatcacaa agccgggcgt gcagtaaccg
    13621 cactgcactg cggcgtggtc gatgaaagct tgttggaccg gatggagttc agtcccatcc
    13681 gcaatccctt caatggtggt aatcaccgcg ttgtgcgcgc gtggagcggg aaccaggcag
    13741 cttacgaccg ccttgccatc caggaacacc gtgcatgccc cgcattcgcc ttcttcgcag
    13801 ccggtttttg tgcctgtcag cccgacgcgg tcgcgaataa gggacatcaa agtctggtcg
    13861 cagtagtccg ggatgaaata ttcctgcccg ttaatggtag tatggatcac cccgccctcc
    13921 cagttatcgg ctggtttgca ggcaaagcct gccctgctat ccagggtggg cacccgcgcg
    13981 ggaaccgacc ccgcagagct gccgtcctgc atctcgctca gggctccttc caccagcact
    14041 ttcagcatgt agttccggta cgcgtctgaa gcgcggatat cgctgatggg caccgcatcc
    14101 gccgctgcca gctctgcagc ctgcgttatg acttcgctgt ccaaagcttt ccccagcaaa
    14161 aattcttccg ccctgggggc atggattaca gtcggcgcga cagacccaag cgtaagcgcg
    14221 gctttctgga tttttcccgc gttgatagtc agcacggcgc agcaattcag gacggagata
    14281 gcctgcgccc ggcgtaaagc atttttcttg aaattcccgg attggtccgc tttcaaacct
    14341 tgaaaacgaa tctcggacaa gaactcatcc gcttgcatga cggtttttcg caagcccgga
    14401 tagaaatccc gcagagggat aacgcgtgtg tttttcgggg agaccagcac cagttcagct
    14461 tccattgcca tcagcggcgt gatggtgtca ttcgcgggcg aggcggttat caggttaccc
    14521 gcaaccgtgc cgcggttgcg cagctgcggg ctggcaacct gcgcgcaagc ctgcagaagc
    14581 ggaaatccat attcccggat gagcgcggag cgcagaatat cgttgtgcgt caccagcgcg
    14641 ccgatatgga caacgccttc ccgatcgcgg aagatatggt ccaatccctg gattcgggag
    14701 atgtccagga cggtctcgag ttccggccat ttgccattgc ggatttcgac catcatatcc
    14761 gtcgcaccgg cgatgatttt ggtttttccc tccggcgaag ccaggcggct taacagctct
    14821 tcgacagtct caacgatgtg gtattgcttc cacatactcg ctcctttatt ctttcgtacg
    14881 ctcggcagct gtgttcctga ggctgataat ttcagccagg atgctcaccg cgatttcttc
    14941 cggagtttcc gcgtggatgt tcagacccac aggggacttt atgcgcgcaa tctctgcttc
    15001 cctcacccca ttttccagca gtccagcgcg cgtggcctcc caacgccgcc gcgagccaat
    15061 cacgccgatg tatgccggct gcgcggccaa aagcgcgggc aagcctgccg tgtccaactc
    15121 gctgcccctg ctgacgtcca cgatgtaggt ctggctgtca atctccagct gttgcggaat
    15181 ttctgccatc ggcatgggca gcagcaggtc agcttccggg aattctgcgc tcgtgcacag
    15241 ctccgcccgg tcgtcggata cgaccacggt gaaattcaag aattttccaa gtttcgctac
    15301 cgctttgcca acgtgccctg cgccaataat cagcagcttc ggccgggcta agaacggttc
    15361 cacatacact tgcattgccc ctccgcaaat tcccagggcg ccttcatccc gggcattcaa
    15421 ggaataactc accaaagcag gcttctgggt gcggagagct tcgagggcgg ctgctttggc
    15481 cgcagcctca acctcgccgc cgccaacggt ccccagcgag ctgccatccg gaaaaacgag
    15541 cattttgctg ccagcgtgcc tgggtacccc accttgcgcg gacaccacaa cgcagaggca
    15601 gacagccttt tgctgctggt tgtactctgc cagtttctgc agaatcacat ccatcagacc
    15661 tccaatagcg aaagcacaat cgggagcagc tgcttcttcc gcgagacgac accctccgcg
    15721 aagcgcgtgc cgtccggcag cgggcggtag ggcagttcat ccagggcagg cggcgcgtcc
    15781 acgaaaatca gccgactcga gccttccacg acatcggtta ccatcaggcc agcaaaatca
    15841 agaccttgcg cgtttttcaa gtttttcaag gcgctttgga gcttcgggat gtagtccccg
    15901 acctcataaa tatcagatgt ttcggcctgc gcgatggcga atttgtaacc agcagtttca
    15961 tataccttca tatcggtggt gaccacctca tcgggtactt gcgtcgcaag cccggcgcct
    16021 gcctgaacaa tcttttcccc gtaggactta atcgtttccg cttccagcgc ggaaccaggc
    16081 acaaatgccc aacgtgccag ccgctcagcg gcggctctat cccgctcggt tgtcgtcggg
    16141 gaagtgagaa tcaaagtatc ggaaagcagc cctgccaaca acagtccggc aacagccggc
    16201 ggagcgctca ggcctgcgtc ttctgtttgt tcggatacaa gggttgaggt gctgccgacc
    16261 gtatccaccg tgaacttaat cggctggtgc gtggattgat tccccaaccg gtggtggtcc
    16321 aatatttcga gcaattccgc ttcttccaat gaggcgatcg actgctgggg ttcgttgtgg
    16381 tccaccagga cgaccttaat gcgcggcgga ttaagcaggt ccctctgccg caccacgccc
    16441 aggtagcgtt tgtggtcatc caacacccag tattcatccg cgtccaggcg cagcaggcgg
    16501 ttgatgacgt ccttaatccg cgtgccgggt tgaaatttcg ggatatcact ttgcgcagct
    16561 tccctgcagg ggatatccag cagatcagcg atggtcatgt cgcggtactt tggatgcggg
    16621 ccgacaattt tccgcaggaa atcaaacaga cttttgccgg ttacaatccc gaaaggcgtg
    16681 ccgtcagtat tcactacggg cgcaatccca ccggtgcggg tcaggatggt ccaggcttcc
    16741 agcagcgact tatccgggct gacaacgtcc aggcggcgca tcaccgactc aaaacgcggc
    16801 gaagcatccg tgagtaagag cggcgcgtcc agttccagca gcttcaggac aaaagcagtt
    16861 tgccgattaa tcgcgcctgc ccgtgccgcg acgctctgaa ctccatcccg ctcgctcagc
    16921 agccaggcat agccgaccgc ggccgcgatg gaatctgtat ccgggttgat atgcccggtg
    16981 acaaaaactc gttcgaggaa tttttgagtg tttccaaagg tttgcatcat caggtggccc
    17041 tctaaacgat tagcgtgcgc gctcgccaga ctttttccgg cgtgatgggg ttttcaacga
    17101 tatctgcgcc gcaggcatcc atcacggcat tccccacagc cggcgcaact ccgtccatcg
    17161 ggatttccgc gactgctttc gcgccaaatg gatgcgactt ttcataggtt tcgacgaaaa
    17221 tggtctgcat gcgcggcatt tcgtccgccc ggaaaatgtg atagtccacc aggtcttttt
    17281 cccgtgcgcg tccgcgcccg tcatagacca tttcctcgca cacgccgtaa cccagcgctt
    17341 gcgccattcc gccctcaatc tgaccggaag ccgcggcagg gttgatgata acgccgccgt
    17401 ccaccgccat cacgagatcc tcgacggtca cctgcccggt ctgcaaatcc accagcacag
    17461 aggcaaattg cgccgcaaag ggcggcggcg agctgggcga aacatgagaa gccactgcca
    17521 tgatttgttc ctggttggta tggtgcaggc tgttcagcgc gatttcagcg agggtataac
    17581 actttccacc gggttcctgc gctttgccgt ccgcaagagt gatgctctcc ggggcggcct
    17641 gcgtgcccgc atccgcgaac atctcgctcg cgcgagcttt gatccgctgc gccacctgcg
    17701 cggcagcttt gatgacggcc gttccggaga tgtacgtcgt cgaggacgcg taagcgcctt
    17761 tgtcgaacgg ggtgaagtcc gtgtccgaag aatagacgat gatatcatcc gcgttcacac
    17821 ccaaaacttc cgccgccatt tgcgccagaa cggtatccga gccggtgccc agatcggttg
    17881 cccccaccag caggttgaag gaaccatcgt cgttgagttt aagactggcg ccgcccatat
    17941 ccaaatatgg gatggctgtg ccctgcatca ccagcgctgc gccaattccc ctgcgcaagt
    18001 gcggctttcc cgggatttca tgccaggagg agtttccata tttttcttgc cagccaatcg
    18061 cttcggcccc ttcccggacg caagcctcca gcccgactgt gtgaatgatt tcaggccgcg
    18121 gttcgcgtcc ttcgttccag gaagtgctga agggctggaa ttcccccgag cgcagtgcgt
    18181 tcttcaggcg gaaggcgagc ggatccaggc caagctccct tgcaatcatt tccatatgcc
    18241 ggtcaaccgg ccagtagccc tgcggcactc cgtatccgcg gtacgcgccg gcgggtggag
    18301 tgttcgtgta cacgatatca gcgtagaaac gaatgttagg cgatttccgg tattcctcat
    18361 ctccaacgta aagcgccatg gatttttgcc cggtgttgcc agttacggtc agggcgtggc
    18421 agccgtaagc gccggtatcg ctcagcgcga tcatctcgtt ggctgtcatc gttccgtcca
    18481 gcttcacgcc ggtgcgcatt ttaatgcgca tcggatgccg cgacctcgag gcgatgaatt
    18541 cttcctcgcg ggacatctcg tagataaccg gcttgcccgt cgcgatggtc aggtgcgcgg
    18601 cgacgtcttc caccagcacc tcctgcttat tcccgaagcc gccgccgatg cggggtttaa
    18661 tgacccggat gcgctttacc ggcagcttca gcagaggcgc cagcatgcgc cgcgcgtgga
    18721 aaggaacttg tgtgctggtc acgatgacca ggcggttgtc cgggtcccag taggagagcg
    18781 ctacgtgcgg ttcaatatgc gcctgctgga ccttcggaac cacatattcc cgttcaaaaa
    18841 cccggtccgc ctcggcaaag cccttttcca cgtcgccaat atcaatgcga atcctggcgg
    18901 caaggttgat gcccgggtcg gaatccgcga aattgacgta ttcgccttcg tcatgaatga
    18961 tcggcgcgcc tgttatcatc gcgtcgcggg gatccagcac cgggggcaat tcttcataat
    19021 cgacttcaat caatgccagc gcctgctcgg cgatttccgc gctttccgcg gccacgaacg
    19081 ccacccggtc gcccacaaag cgcaccttgt tatccagcga gaagctgtcc agcgggcccg
    19141 gaatcgggtc agactgtccc gcggtagagt acaccacgcg cggcaggtct tcccaggtca
    19201 gcacagccgc tacccctttc aaggctttcg ctttatccgt gttgaggcga acgatgcggg
    19261 cgtgcgcgag agggctgcgc aggacgcggg ctacaagcgt gcccgggcgc gcaaagtccg
    19321 cggtaaaagc ctgcctgcct tgtgccagct tttcggcatc caccttgact gtagatttgc
    19381 caatgacctg agtggatgga atggcatcaa ccggcacaat tttgggctcg gagacggccg
    19441 cgcggttctg ctgcgcgcgc atcattgccg ccgcctgtaa gaccgcttcc accggtttga
    19501 cgtaagccgt acagcggcac aaaatgcctg aaagcgcgcc gcgaacttct gcttcgcttg
    19561 ggtcaggatt gcgttccaac agcgctttcg cagccaggat catggcagag gagcagtaac
    19621 cgcactgaat ggaaccgttc tgaataaaag ccttttgcag cgggctgagc ccttcagttt
    19681 ccttccagcc ctgattgggc ggcgtcccaa gagattcaat cgtgacaatc tcgtgcccca
    19741 gcgcctgtac agccaggaac atgcagctgt ttaccgcttt tccgtccatc aacacggtgc
    19801 aagccccgca ttccccgcgt ttgcatccgc cagactttac gccgaggtaa cccaaccggc
    19861 gcaaaacatc cagcagcttt tcatcaggcg cggcttcaac ggcaacggac tgtccattga
    19921 tatggaggtc gaagttcata attcctcctt tttgagggcg ttggattcga gccgctgcag
    19981 gcagcgcgaa agcagcacct ggctcatctc ctggcggtat tccgcgcttg cccaggcatc
    20041 cccactggct tcatacgcgt gccggaccag cgcaaataag gcttccgcct tggcgctctg
    20101 tgaaatcacc cggggagggt gctcgtcacc accgcaagct atgcgcagcg ccgagtcttt
    20161 tttgcgtgcc gctgccaggc tgatgatagg cctgtctttc ggggtgcgcg cgatgaattc
    20221 ccaggcgaaa tcatctggta ttcggaaact catccacaaa ggaaattcgc gttcgttttc
    20281 gggttggctc cgcggaaaac gctccagaga aacaaacctt cccgaaggcg ccaattccag
    20341 ctcaggcgcc agcgcgagca gcgcggtcag gaagggagag cgctggtcag cgctgcgcaa
    20401 aaagttcagc aggctcagac tgctgcgcgc gtttgcgccg gcttcaagct ccagcggggt
    20461 gcgcagttcc tccagcccgg tatcagcttc cagaatttgt tgcaggctgg ctgccgcgcc
    20521 gatgcgcagg atgtccccag atttttccac ccggtcagcg cctaaatcct gtatatcgac
    20581 aaataggtca gaggagccca gcctggaagg cacatacgcc ccgccggcca ggatgcgcag
    20641 gcatccgtgc gtttcgcgga atagcacggc taattcttca aggtttttcg gccttttaag
    20701 gcgattttca tcatttctca tcaggaatca ccctccaacc aaatgagtct ggattcattt
    20761 cctgaagctt ttcctcaggc atatccacat agataccgct ggcttctacc agaaccgcgc
    20821 cggtctggtc aagcaattca ccccgggcaa aagccacacg tccctcgcgc ttgaccttat
    20881 accctttggc gattaacggc gtgttcaacg gtaccggttt ccggtagcgc agcgtcagtt
    20941 cgctcgtgac cataaattgg tcgccatgat ccgagaacgc tctgccgcaa atctcatcga
    21001 gcaccgcggc agtgaccccg ccgtgcacaa tccccggata tccctggtat tggtcgccaa
    21061 attgaacata cgcgatggtg tcgccgtttt catcgacgaa aaaactcagg tccagaccga
    21121 ctggattctt ccgtccgcag acaaagcagg tgtcggaggt ggcctgtttt ttcatgcttg
    21181 ctccggagca aaggtatcta tcagcagcct ggcgttgccg ccaaccgcgt acaaaatcgg
    21241 gcaggtgcag ccgtggtcga tatattcctg aaccttggcc ttggcctcgg aaggcgtacc
    21301 gctggcagta atcctgtgga tcagatcctc cggcaccaag tgcttggctt gttggatttg
    21361 ttcgtgggta gccggccatc ccaggattgc ctggattttc tggacaactt ccgtcgacac
    21421 tccggacgct ttggcaatat gcggctgctg ggccaggtat tggcagagca gttcgcgggt
    21481 ggtatcaatc gcgcggtcgt ggtcttcgtc cacggagcac accaccaact gcggacggtc
    21541 aaagtcttcc aggcgtctac cggcaatttt caggccgacc atcagctgct cgatggcgat
    21601 gtcgttgtaa ttcactggca cgcagtaatt catgaccact ccgtcagcaa tccggccggc
    21661 catttccatc attttatcgc cggtcgcgcc aatatagatg ggcacatggc ggggttcgcg
    21721 cctgccgtgt acgacgtcca actcaatacc gtgcacgttc acgaattcgc cttcaaaggt
    21781 cacgcgttcc atattaagca atttgcgcat tacggtgatg gtctcctcca tcgctttcag
    21841 cggcttggtg cggttgatgc cgacgttctt cgccagcggg tcccaccatg cgccaatacc
    21901 gcaaatgata cggttaggcg ccaggtcgtc gagcgtcagg aaggtggaag ccagcaagcc
    21961 gatgttgcgg gtccagttgt taatcacccc ggaaccaacc tttatgcgtt ccgtcacggc
    22021 agcataggcc gccatcggga cgatcgcatc ccgaaccagc cggctttccg cctgccaaac
    22081 cgcctcaaag cctttcgctt ccgcgtaccg gacgatgtcc agtccttcac gcaaatcatg
    22141 cgcgtcctgt aagtaaattg caacacgttt tgtcatggtt ggtccttttc tgagtttcta
    22201 tctgtttagc ctgtcaaatc aggctttccc gtaaataaag aaggtcttcc ggcgtgtcca
    22261 ggtcgtgctg aatgtgcgta ttcagataaa tgacatgctg gagtctgcgt tcttctgcca
    22321 gtgcgcagtg cagcgcgcag gaattatttc cgaatgcggt gggcatcagg tcagaccggg
    22381 agaggaaaag cgcatttgtt cctgtcatgc ggtagtccgg aaccagtagc atgccatttt
    22441 ctgccggtgt aaaaccagat aacagacgca aatcctctgc tattaacagc ggcaaatcag
    22501 tcgggatgat cagaacagag cccgcgctgt ccgcctggat ggtctggaga gcctgccgca
    22561 gcgcagaatt gaggctgccg gagggctctt cgcgcaaagc gaaaaagcct tgctccaaag
    22621 cccatgccag cgcgccttca tcctcgctga tgaccatcat atgcctgaaa atgccgcttt
    22681 ctttggccgt ggaaagcgtt cttcgaaaca gtttttggtt cacagccgcc agctcccgcg
    22741 ctgagagaac ctcgtgcagg cgggattttc ccaaatcaaa tcgtttaacc ggaataagtg
    22801 cccatgtaat cacggcgctt tccttacaat gaggtctaca ccaaagcgca gcacttcagc
    22861 cgcgagcctg gttttgtctt catcgttttg catgagagtg tgcgtaccta gtaatattat
    22921 acgctcgccc tcataatttt ggaacaaacc gctgtccagt tggtcaaaaa caaagccgtc
    22981 cagaaagtct tcataatggg caagcacgct cggactgcct ggctctatgc ccaattctgc
    23041 gaacatcttc gccgcaggcc cttttaccgt cttaccctga ataataggtg agaccgccag
    23101 cactgttttt tcgcgcaagc tctgcctgat gcccggcagc gcgagtatcg ggtcgatgct
    23161 gacccaggga ttggaagggc agagaatcac caggtccgcc tcttccagcg ctgtgagtac
    23221 gcccggcgcg ggctggacgt cctccgcgtg cgaaaaaaca aagcccgaaa cagcaggcgc
    23281 gcacttctcg cccacaaagt attcctgaaa gtccagcaaa ctgccgtcgc tggtccggac
    23341 gcgtgtgcgg aaaagcgcgt ccgtcatcgg caaaacacgg ctcctcaccc cccaacgctc
    23401 gcaaaaatgg caggtaacag cgctcaaatc ccagcctgcc tgcagcaaac gggtgcgctc
    23461 caggtgcagc gccaggtctt tatccccaag ttgaaaccag gcaggcgcgc ccagttccac
    23521 cagcgcgctg aagctgttcc aggtctcgtt tgccctgccc caacctttca ctgggtctgc
    23581 cagcccagcc agagcataac agaccgaatc caggtccgga ctgatacgca gaccgaaatg
    23641 ttcaaaatca tcgccggtat tgacgatgac ggtcagattg cccgccggca ggaccttatc
    23701 cagcccgcgc gcgagcttgg caccacccac gccgcctgcc agggcagcca ccttgagagc
    23761 agaaaattca gtgaagtccg ggtgcattag cggaacaggt cgtacttttt gtcgcgcacc
    23821 aggctggctg cctggtcatc cgccaactca tagggaaagc cgcgcgccaa cacgaccggc
    23881 gtgccttcgg cggcctgccc catcatcagg gatgcgccag ctgccaattc gtccgccgcc
    23941 gcgatgacgg tgacgcgcag cgcataatca aagcggtcgg gctttccgcg catgtccacc
    24001 agcgcgggca tacctgccag gccaatgctg atgccgacga tgccgttgcg ccaggctctg
    24061 ccgtgtgagt cgataattaa tacgcctacc cgttttccgc tcaggcgcac gagaccttcc
    24121 cgcaaaaccc gggcggaata atcggcatcc tcaggcagga gcagcaccca gtcctcgggc
    24181 gcgccccagg gaccgcgtac gttggagtgg tcgatgcccg cgttggcgca cacgaatccg
    24241 cttttatgtt cgacgatgat tgtgcccggg cgcatgcgca gtaccgcgct gctttcgtcc
    24301 aggataagct ggaccagctc gggtcgcttc ccaatttgcg ccgcaagctc cagcgcgcgc
    24361 ggtccgggcg ttacagtgct caggttcacc agcctgccct cggattttga cacgattttc
    24421 tgggcgacca cgagaatatc cccatcttcg agcgccatcg cgtgttgagt gagcgcgccg
    24481 atgatcagtt cgggcaagtc gtcgccagga tgaatctcag gcaggtccgg tacagtacag
    24541 agaatcaggc ttttgggctg cagcattcta ctcaggaatc cccgtaattt taatgccggc
    24601 tgaactgacc ttatgctgga tattcagccc gatcaggatt ggggtcagcc cctcaatcac
    24661 gacggagttt tcaatcacgc ccgcatccca gccccgcaaa ccggcatcct ggaccagacc
    24721 aatcacggtc cggcgggctt ctttacccgt tccggcgaca agaacatcac aggccacgct
    24781 ttcatcgttc aggaggtgtt cataagcaat gttctgaaaa gcagccacaa cctgaacgcc
    24841 ttcccccagg attttctggg cttgctgcgc ggcggaacct tccggaggaa tggaaacccg
    24901 tgtggctttg gccgggttaa gcggcacaac aacgtcgatc aggattttcc cctggagggc
    24961 gtctttcaga ccttgcaagg tctcagcgtg tgccgtgaag ggaaccgtca gaacagcgat
    25021 gtcgcactgc tccaccgcgg ccgcgttgct aaaaccttcc acgcgcgctt ctgccggcag
    25081 cagcgccttg agctcggctg ccgccgtccg cgccttctct tccaagcgcg agccaatcag
    25141 aaccttatgc ccggcttttg cccagcggta cgccaggccc tttccttctt tacccgtacc
    25201 gccgataatg ccaattgtgt atgccattgc gtttctcttt ctattggatt tttttccagg
    25261 tacgcagggc tgcttcgcgg gcttttgcgg cgatttccgc ctcatccagc cccagcagct
    25321 tccggtcgcg catcagcagt ttgcccgccg caattgtgct ggtcaccatg ccctcgcgga
    25381 aaccgaacag gatatgccag ggcagatttc ctgcgctcag cggggtgatt ggatggtaat
    25441 cgaccagaat caggtcggct gctgcacccg gttctattat gcctacgcgc agcccatcaa
    25501 aggtttgagt tgccagcctg gaattattct caaccgcaat cttcatcacg tcgtaaccgt
    25561 tcatcaggcg cgggtcgtga ccatggtctt tctggatgaa gtacgcggct ttccactctt
    25621 cccacatggc attggagaat ccatcgttgc cgagcacgac gttcacctcg tgtttaagca
    25681 tatccagcac cggcgtaacc ccgacggcgt tgttcatatt cgagcgcggc tggtgcgaaa
    25741 gccaggtgcc ggtctccgcc agtaaatcga attcccgtga attcgtatgc acaccgtgcg
    25801 ccaggatgct tttttctccc agaataccct gctcataaaa gcgttcgatc acgcgcttcc
    25861 catacttccg caggctgttt tcctggtcca
//
LOCUS       DAOQAD010000018        24523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_2042_length_24523_cov_5.570419, whole genome shotgun sequence.
ACCESSION   DAOQAD010000018 DAOQAD010000000
VERSION     DAOQAD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24523
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2042_length_24523_cov_5.570419"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /gene="rplU"
                     /locus_tag="PKL60_02825"
     CDS             complement(<1..197)
                     /gene="rplU"
                     /locus_tag="PKL60_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNW95101.1"
                     /translation="MKYAIVEAGGKQYRAEEGKTITVDLLPANVGDAVTLDSVLLIAE
                     GDRVEIGTPTVKGASLKTTVV"
     gene            534..605
                     /locus_tag="PKL60_02830"
     tRNA            534..605
                     /locus_tag="PKL60_02830"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:567..569,aa:Glu,seq:ttc)
     gene            complement(796..2367)
                     /locus_tag="PKL60_02835"
     CDS             complement(796..2367)
                     /locus_tag="PKL60_02835"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNW95102.1"
                     /translation="MNPTEATLPNPTTTSAPTATATPEPIYLQLDADIPADFSRSLNL
                     EGTDFQVVDSGKNAAVLDFGPGNPVSFWVYALAAPFPTITDTIPTKTFLDFWKRAGAA
                     PFSRLIMDAGTLKALQSRWGVPAGNVEVLDAESLMLEAWTHADTWAVIPFEEIQPRWK
                     VIALERQSPLHKDFALQSYPLSIPISLTASVGFQSRDLAERLKPYLAQSNRDPEKLVT
                     VILTGVTAMVRGTASEMEEKGITRGAEVILPVTQAADILHISNEVPFAVDCKDPVPKL
                     SGKLSFCSKDSYIKLLEAVGTDVVELTGDHFQDWGEAALEHTLELYKQEGWLYYGGGL
                     NIEEAKKPLKLEIKGTKIAFLGCNAKPTVYGNATEEKGGNFRCDWDYMTAQVKSLRAE
                     GYLPIVTFQHLEFYQWTAQSQLVADFHKMAEAGAVVVSGSQSHQPHGIEITDGAVLHY
                     GLGNLFFDQYGLATFTNYGFLDRHVFYDGKYLGVELLPIQFKDYSQPVWPEEPTRAWL
                     MEILFQVSEWNYPPK"
     gene            complement(2653..5697)
                     /locus_tag="PKL60_02840"
     CDS             complement(2653..5697)
                     /locus_tag="PKL60_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNW95103.1"
                     /translation="MNHKRIPDYFRTILIFLSLLLLMGGLTRAASAQTSPVDWLAFGS
                     SDTRQAPEVALLQADAESITLRASADGVGVLPVQIGGQTYLNLTGEGFGTRAEPGAPA
                     LPVILRDVEVPFGAHVSVEVLSAPSRFTSLADLGLEGVIAPRQPSQSKCAEAQGEVPP
                     DSQIYALQEAYPGELVRIVDEYIVRGHRVVQLAIAPVQYTPASGGLETYSSIEFALKL
                     EGSDMAATYAEAERLNSSGFNSIYAGTVLNFNQGRPLADPKDPENYLIITADAYESGL
                     SAFVSLKQSQGFNVSVANITTVGGNTTTAIKQYIKSQYLGATPPDYVLLVGDYNNGAD
                     SITNYSFRSESSSYRTDLYFFTMDNETEYVPDIFYGRFPVRSTAQLADMIAKLQTYAG
                     RSGQEAWVKKAAFLATSDTGWYDFAEATQNYVINTYTLPKGYTGTFPNNPMPGGDKLY
                     AITYGAGSANVISSINDQRAMVIYTGHGSQTGWAGPSLSQSQVRSLTGVAVPYVASHA
                     CMTADFTVNESFADTWVIEPEYGALTIAAASNYSYWYEDDTLERTIFNSLYADPTGAV
                     VPSVREMVHAGLVAVDASTTNRDQYYWEEYHIFGDPSLEIILGPRLPDFTLSLQPNTL
                     DTCSTDTHEVTVNVGSLNEYDEPVALVNGQLPGFSLSFEPAGPVTPPGQAVLTLEGNG
                     SASSGSQTVAVTGTSGSLTHQADLLVNVFNPLNSGPQLVSPADGAVNIAPGTGFAWQA
                     LGGAVSYRLEVALDAAFSQVVLTQAGISGTTYSPPAALQTDTLYYWHVSAENPCGETT
                     DNQVFSFRTRPGPGDCPAGTTAQTLYLGDFEDGAPGWTDTSTGAYHWALSTVKAHSPV
                     SSWRGAVPSVIADQRLVSPGFDLPAVDLPLSLSFWHRWTFDSSSACNDGGILEVSTNG
                     GTTWSQLSSSKLLTNPYNGTVRSGVYNPLAGKSAWCGVSDWVWTVADLGDYAGQTVSL
                     RFRLGSGNSGSAEGWYLDDVKLQSCVAEPIEETLIYLPWLSR"
     gene            5968..7491
                     /locus_tag="PKL60_02845"
     CDS             5968..7491
                     /locus_tag="PKL60_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNW95104.1"
                     /translation="MLAHVFSCAVIGLDGVVIDIEVDFGQGLPHMAIVGLPDEAVKES
                     RERVYSAIKNADLAYPRKALTVNLAPASVRKEGPSFDLPIALGVLIAGEQVSQEQIEG
                     ALVIGELSLDGSVRHVRGMLAVAAAARQAGFKSLFVPAEDAPEAALIPDITVYPVASF
                     AGLYLHLIGEQPLTPAAAVPFSPQSPQPIETDFSEIKGQEHVKRALEVAAAGNHNILM
                     SGPPGAGKTLLARAINSILPRLTLEEALEVTRIYSICDMLPADTPLMQARPFRAPHHT
                     ISHAGLVGGGNLPHPGEISLAHRGVLFLDELPEFGQRILEVLRQPIEDKRVTISRANG
                     TLTFPANFMLIGAMNPCPCGYYGDTLKQCTCAPAAITTYQKRISGPLLDRIDMLIHVP
                     RVDYQKLSSSRQGESSAAIRERVELARQRQSERFAGTALFSNADMSPAELRRFCALEA
                     PTQALMQTAMRQMQLTARGYHRVLKLARTIADLAGSAEIGQIHLAEALQYRAKEFEF"
     gene            7583..8500
                     /locus_tag="PKL60_02850"
     CDS             7583..8500
                     /locus_tag="PKL60_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNW95105.1"
                     /translation="MRTIQLGSSNMQVPVIGVGCMRLKTLESSAAEAFVQSALALGAN
                     FFDHADIYGGGICEEIFAQAVHMSPSVRENLILQSKVGIRKGMYDFSKEHILSAVDGC
                     LARLKTEYLDVLLLHRPDALVEPEEVAQAFDELEAAGKVRHFGVSNHKPMQIALLKKF
                     VRQPILANQLQLSIAHAGMISNGIHVNTNEDAALDRDGSVLDYCRLHDITIQPWSPFQ
                     HDMFKGVFVNNPEFPELNACLDQLARQYGVSSTTIALAWLLRHPAKMQPIVGTMNPVR
                     LKECVQAADVHLSREEWYNIYSAAGYSLP"
     gene            complement(8584..9012)
                     /locus_tag="PKL60_02855"
     CDS             complement(8584..9012)
                     /locus_tag="PKL60_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95106.1"
                     /translation="MLTLLGAIAAAVGIFKARKWGVILGIIIAALLLISGVLGLFGAF
                     GGLNLIIPIGKILLAAGVIILLLLPAARRIYAAPKAQEDDNEDDDDEDEAEGTGAAGQ
                     DGQASGEAVQPEAGEKREVNEAALYEEPSAAVPTEETPVE"
     gene            complement(9361..10572)
                     /locus_tag="PKL60_02860"
     CDS             complement(9361..10572)
                     /locus_tag="PKL60_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95107.1"
                     /translation="MSIFETFRIAFRAISANKLRSGLTMLGIIIGVAAVVALMAAGQG
                     ATSGITSRVEGMGTNLITISTGRSFQFMYGGQSQQLLYSDFEALMEDLGASGEIAPYY
                     QGSASVMNGDRSANASVRGVTPEYIDVNAYELAKGRFISAEDNFQKQRVVVLGSSSAE
                     DLFAGLNPLKREIKINGKSFEVVGVLAEKGSNDDLVLIPLQTGYSLVFGAEARSKGQY
                     TLSSISLSAYDAEQVDTLIARIEFILRREHGNKLTDELPFNVSSQSQMLESLSSISST
                     LTTLLGAIAGISLLVGGIGIMNITLVSVRERTREIGLRKAVGATRGVILFQFLVETLA
                     LSLMGGLLGVLLGWAIAWGVSASGLLTATVTPNVVFLSVAMATLVGLVFGLYPAFQAA
                     GLRPVEALRYE"
     gene            complement(10569..11333)
                     /locus_tag="PKL60_02865"
     CDS             complement(10569..11333)
                     /locus_tag="PKL60_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95108.1"
                     /translation="MIETQEVSKTYRLGDTKVRALRKVNLKIEDGEMLAIIGPSGSGK
                     STLMSILGCLDVPTKGVYLIDGQPVESLSENQLAAIRRNKIGFVFQQFNLLPRTTAIE
                     NVMLPMYYSGVRPKVRRQNALEALAIVGLSERAYSHPNQLSGGEQQRVAIARALVNKP
                     TILLADEPTGNLDTRRGDEVMQIFQKLNRQQGQTVVYVTHDPFIARHTNRIIQLVDGR
                     IVSDEAVLQPIEAGMPRPDDLMVKSWEGNSDEEEDA"
     gene            complement(11343..12821)
                     /locus_tag="PKL60_02870"
     CDS             complement(11343..12821)
                     /locus_tag="PKL60_02870"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNW95109.1"
                     /translation="MKKHGIIYFLVFLTGAGLVSGCANSASSASTTTNDIGQVTEITL
                     TNTVEASGSVTPRQIATVNWTLSGTIATVPVVVGQQVARGDILMTLDTSSLPESLKKA
                     QEQLAEFTSPSAVAAAQQKVLEAQSAYDTAVQNRKYLDYQDEDVIENAHAEYILAKAK
                     YDDAYENYQNWEDEDYSDHYDTDLATAYTALYQAKTEMETAEYLYDLYKSQSSAQTYS
                     EYDNAVLVTGSALTEAQNYLTLISGGDIPDDATSASIQTYRQALETLDGINLRAPFDG
                     SVAAIYDEPGILVSNNHTSVKLIDRSALYVGISLEESEVTQVSVGLPATVTVDILPEL
                     PLSGHIWSIDPVGTVSNGMVYYEVEVELDKTNDAIPINASASVSIQIGEPETKLLVPA
                     SAVQSDDAGEFVQVIRNGSYERVDVVSGTILSDDTVVVSGDLTVGDFVLLIIEVSSTE
                     ESSGAGLGILGGGGNRGEIPSGGGEMPQGGQQPPSGSAPQNP"
     gene            13188..13919
                     /locus_tag="PKL60_02875"
     CDS             13188..13919
                     /locus_tag="PKL60_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95110.1"
                     /translation="MRKTLPMLCIFMGFTLSACFPQTAQEPQVDYSPTLSSYAVEVNS
                     LQRDIQDLEADKQALTTEKEALVAQITQLQSQAAQSAESAARITELEGQVDNLQSQLN
                     DAQAGLNGFGAMVAGYEEILMGEYYDEDGLMVLCPDAFDVSFGYVDRVSMRKQLVDYI
                     YNQYVNDGYTVDINTITSEHQQIWTDEQDALVKIHILDFLEPFMVTFENPEYGIYSAV
                     YSVRWQCFVDHPVLEARYLALHGNE"
     gene            14082..16979
                     /locus_tag="PKL60_02880"
     CDS             14082..16979
                     /locus_tag="PKL60_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNW95111.1"
                     /translation="MASTVFGRKYHWQLREQIGKGDAGEVLRVQTELGRERGLMKRPV
                     QNVSGGTILRQAVQIENEGKVLAALNGLDVRRNNLTVHTPLLLDQSPEGTSKTAALFI
                     VSEEVRGIAISNLLKNLLQKNASFSQTLVLKVLSSLFTLLSRVHALGIAWNDVKMEHI
                     FWDETTNTLSFIDWGNGLFFDPDEAQHPQHPVRLDYQQFVSEGSLLLNQIAPDLISEI
                     DWPQEDANLTPEALLQLQHRVEYMEAHLSMRIKEYQLAFDKSSRQINSLAELQDILEL
                     KQALGRLGVQVQTQTALQSVRRFALSLAQANAYPEVHALVELLRTYLPEDASQSWQLV
                     DYLLSLEDAWDQPAFPVVLEKTIANDLPAAIWELEGARESSGNLPRLINIQLAMRSAA
                     GVPDALRDTLLEQLRAWLDDTYARLLHTRQEFPAEIDAAAALETFASNLDALLSNWQN
                     LGAGEMLGDKLLLLRDLLRSPGAHLTNLPQSLSQALNLELAKTREILTFWTEGELNKS
                     RDALKNFFLLEPTMPYLREISAGIEALKGWMDELSRGPQPWQSVNAFGRGLLERKPDL
                     AKFLSQPAWLASLVQCAEALAAASDGEMLRLLAENNQWPIPWVDYGEITIEFRTAAAS
                     TRMNAAQKDALAGFHQALCGDQDLPQALSRLKSLLPGLYGQYRLLAQGFETLFTPLPA
                     QTQAVEAAAFPPADRQNVVQALEVLDYLKRWKESQLDAGTAMGAEPPAAFQGWHLVTA
                     AHNAQRTWHAALQPALTLLKQMRWEALAEFTRANKLPDALTACVQALAQLVADWRRLQ
                     ETGVFPENVREMISLAEQAQTQFFVFWQEQEQAPSRPLRWLIQMNQSAFSGIHQSLHQ
                     LTRHLQTLERTLLALARPGVARSRLAWNGAGDMMFALGQISALLEPPSRKPSRVRLWQ
                     EQYNQVLKQTEWKNAASLLETLDPAHPLAAWFQELASKDLDIFHETPRQKW"
     gene            17003..18013
                     /locus_tag="PKL60_02885"
     CDS             17003..18013
                     /locus_tag="PKL60_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95112.1"
                     /translation="MIEKKLTPQNGFYLAEPLDTWLTAGNRDYAIPEAAGSSARVFLL
                     KDESNSGGYKRDPAFKVMRYDKISYARPLFLEEYKILAGLNGIQGLTSMLQGGFLKPD
                     PGCVWPSELAPLSKDAEQNASALQVSGKLAIFKPEETDDVLQQMDERLADGWLPFLIL
                     ERRWEDNLYMLCDAGYTRGNFAKNLTMKQVLDISIQICDILAQAHQRGATFLDHKLLH
                     YYWNEYRQKVIMIDWNIGHWQPENIQPEVLQFDLVQFSSRALHHIFTGRQAPGSVAVG
                     PNRPEDIASAPRHFKASYSYDVQNRLNQEEMRFLEKALDGAFLSAAEMREHLQFLASK
                     RL"
     gene            18051..19874
                     /locus_tag="PKL60_02890"
     CDS             18051..19874
                     /locus_tag="PKL60_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95113.1"
                     /translation="MILDLIEQAKALLKKNNPADDELLQVENALSTYLEAVATHRISD
                     QVDLDDIDEANRLLREIRRERGRRIASQAEPAQTETSTSTSKPAADQPPMPKPAVNRL
                     DLQSDAGIDPLRKFFQSSHDPEAERMMDEAEELFYKGNYQAAIPLYEKVMQMEPGWSR
                     AEEHHSEAEEFLRSGNIPSVALPPEAGKAYGKAQSAARVFRYQVALNYLDEAFDHLQA
                     AGIKRWREGEELRHDLENQMQAYDVYKEGLNLLSQGELVQALGKIQTAASAVAIPEYL
                     DKASEVRADISLLEEVSDIVSLSGKIPPGKLADAKSKLERLRMKYGSISQISRLQNRL
                     DLLIPATIQSLLDNTRRLSKEAAEAPTLANALQKIAGARENLDLLRQLDAYDSQSLLL
                     ENEISSLESEIEANQDAINRALEALKSGSRFFALNAFLISRGVRKRFPNDPQVITLKR
                     GLSLSYLVAGLGGLLAILVLGLLISVATRSISSSIQARNLARTPTATFTPTITLTPTI
                     TPTPTQTLTPTPDYSPTPTKTATPTPVMVVETVRQVYARNDCYEGYIATGRIPQGTLV
                     TLLPMPERRFDNFNRECLLVEFRGNGFSIIGYVLLVDVIAK"
     gene            19970..21349
                     /locus_tag="PKL60_02895"
     CDS             19970..21349
                     /locus_tag="PKL60_02895"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNW95114.1"
                     /translation="MENLEQAYQESLDFIYSFIDFSMKRHLDDSHRFFKLDRMRRMMD
                     LLGSPQENFLSIHVAGTKGKGSTASLCASALRAAGFKVGLYTSPHLQEFTERIQVDGA
                     RIEKQALVDLVARIRPLTAQVPEITSFELMTAMAFQHFSERRVNIAVVEVGLGGRLDA
                     TNILTPLVSVITPISYDHTAILGSTLTEIAYEKGGIIKPGVPVVLSPQNPEAEQELAR
                     IAAERGSPLTRVEQDYALLILNHSLRGQELAITAGPSSPSRAHSLATAPLNLHLPLLG
                     AHQAQNALTALGGLDQARVQGLALSREDVRKGFGQVEWPARFEILRENPPVVIDSAHN
                     GDSMNRLRQSLDDYFPGQKFLLVFGASADKELDAMLKAILPRVERVITTQSTHPRAAD
                     PVHLMEQIRPYGLPVEACNPAEDALARALELAGTDCGIIVAGSVFIAAAARDIWFSSS
                     NGRVAVLRV"
     gene            21478..23928
                     /gene="lon"
                     /locus_tag="PKL60_02900"
     CDS             21478..23928
                     /gene="lon"
                     /locus_tag="PKL60_02900"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNW95115.1"
                     /translation="MKDRKPDKDGYLVGPAIDLEEMLLLPHAMMPLTLAEDPNAMDTL
                     RDAYQNSETLFACFSRQDFNHLTETAEPEKRYLEIGLEVAVGSLISLDNDRESVLLQA
                     RNRVKIEDIWQEDGLLMMRCLPVKSRQRKSNSLTALKRAVVNEFERYASLVEFINDDV
                     VEFVRSVEDPGDLADMVLSTLDLSEADRLAFLIETDGAARLEASLRNLKHEVRLQELE
                     AEIQNRVQSSLDQNQRENYLREQLTTLQRELNDGKPIDPEYQELSEALAKISLPQEAR
                     ETVNKELDRLANTPPFSPENALISAYLHWVAELPWDKETADNLDINHAREILEKNHFG
                     LQKAKDRILEYLAVRSLKPKRNRQPILCFVGPPGTGKTSLGKSIADALERNFVRVSLG
                     GVHDEAEIRGHRRTYIGALPGRILQTMRKAKSVNPVFMLDEIDKLNADFQGDPAAALL
                     EVLDPEQNYAFSDHYLEVAYDLSKVLFITTANTVSSIPPALLDRMELIEFPGYILEEK
                     IAIANQFLIPKQMEENSLDGEPILFQEEALKALVEGYTYEAGVRNLEREIGSVLRKIA
                     RMKSEGKTLPAEISPELVEELLGPAEFFPAVAEQNDEIGMATAIAWTENGGEIMPVEV
                     LVMEGKGNLQMTGQIGEIMQESAQAALSYLKSKQEAFQIPADFFEDMDIHIHVPEGSI
                     PKDGPSAGITLATALTSAVLAVPVRHDVAMTGEITLRGRVLPVGGVREKVLAANRSGI
                     RTVLLPRKNEKDLREVPPQVLKKLQIVLVEHMDEVLAAALVDKPISRKPRAASSRAKK
                     KKSASDQQDAPQASETSC"
     gene            complement(23929..24396)
                     /locus_tag="PKL60_02905"
     CDS             complement(23929..24396)
                     /locus_tag="PKL60_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95116.1"
                     /translation="MDILHLVDRLEELFNNSRPIPLSRSVVVDENAFMDIIDQMRISI
                     PDDIKKSQQIIAQKDRILAQAQEEANRTLALAREKSEKMIEKSDVYLAAQAKAEQITE
                     QARREAGQTQLDADRYVVETLTKLEAEMNRILAQVQNGIRALQAEDRTAPPEV"
BASE COUNT         5371 a         7259 c         6689 g         5204 t
ORIGIN      
        1 tccacaaccg tggtcttcag gctggcgcct ttcacggtgg gggtgccgat ctctaccctg
       61 tcaccttccg caatcagcag aactgaatcg agggtgactg catcgccgac gttcgcgggc
      121 agaaggtcaa ccgtaatggt tttcccttct tcggcacggt attgtttacc gcccgcttca
      181 acaatagcat atttcatttc gtctccaatc ttctcttaac cgcttttcag gcgtgctgcc
      241 gcttgagtgc gggtaagttg gtttacacaa tgaaccccag cctactttcg ccggggcaac
      301 gaaccagtag tatatatcca aagcccttcc ctgtcaagaa atccgggggg aatgtcgtgt
      361 aaggaagatc gcttatccag gccgcccact tccgcggttt ccgctgagag aacctgcggc
      421 gccggaaaag ctaaggcggt cttctaggat tctaccgcaa aattgccctt ttttttgccc
      481 ctttttcgct tgacctgtga aaaacgggat gatagaatat ctctcgctta actgccccca
      541 tggtctagcg gttaggacac agccctttca aggctataac tcgggttcga gtcccgatgg
      601 gggcacacta aggggctgtc caactttcag ggcagcccct tttggtttcc cactccttgc
      661 ccgttttatt cattgttggc agctttgcgc tatcaaaaca aggcctggac ccggtacgct
      721 ctcccacttt gggctgtccg aaagaaaagc ccgttctttg aggcagcctc tttcagtttt
      781 caacaccaaa gcggcttact tcggcgggta attccactcg ctgacctgga agagaatctc
      841 catcaaccag gcgcgcgtag gttcctccgg ccaaaccggc tgcgaataat ccttgaactg
      901 tatgggcagc aactccacgc ccaggtattt cccatcgtag aacacatgcc ggtccaggaa
      961 tccgtaattg gtaaaggtcg ccagcccata ttggtcgaag aacaggttcc ccagtccata
     1021 atgcagcacg gcgccgtcgg taatctctat gccgtgcggc tggtgcgatt ggctgccgct
     1081 cacaactact gctcctgctt ccgccatctt gtggaagtcc gccaccaact gtgactgcgc
     1141 cgtccactgg tagaactcca gatgctggaa ggtgacgatc ggcagatatc cttccgcccg
     1201 cagacttttc acctgggcgg tcatgtaatc ccaatcgcag cggaagttgc cgcctttctc
     1261 ctcggtggcg ttgccgtaaa cggtcggttt cgcgttgcag cccaaaaagg cgattttcgt
     1321 gcctttgatt tccagcttca agggcttctt ggcttcctcg atattcaatc cgccaccgta
     1381 atacagccag ccttcttgct tgtagagctc cagagtgtgc tccagcgcag cctcgcccca
     1441 gtcctggaaa tgatctccgg tgagttccac cacgtctgtt ccgacggctt cgagcagttt
     1501 gatgtatgaa tcttttgaac agaaactcag ttttccggag agcttgggta ccgggtcctt
     1561 gcagtccacg gcaaaaggca cctcgttgct gatgtgcaga atgtctgctg cctgcgtgac
     1621 cggtaagatc acctccgccc cgcgtgtgat gcctttttct tccatctcac tggcggtgcc
     1681 gcggaccatt gcggtcacgc cggtgaggat gacggtgacc agtttttccg ggtcgcggtt
     1741 gctctgcgcc aggtagggct tcaagcgctc cgctaaatcc ctggactgaa agccaaccga
     1801 ggctgtcagt gaaatcggaa tagaaagcgg ataggactgc agcgcgaagt ctttatgcag
     1861 tggagactgc ctttccagcg cgatgacctt ccagcgcggc tgaatctctt caaaggggat
     1921 gacagcccag gtgtccgcgt gcgtccaggc ctccagcatt aaactttccg cgtccagcac
     1981 ctccacgttc ccggcgggca caccccagcg gctctggagg gctttcaggg ttcctgcgtc
     2041 cataatcagg cgggaaaatg gcgcagctcc tgcccgtttc caaaaatcca ggaaggtttt
     2101 ggtgggaatg gtatcagtaa tggtcgggaa aggcgcagcc agcgcataca cccaaaaact
     2161 gaccgggttg ccaggaccga aatccagtac agccgcgttt tttccgctgt cgacaacctg
     2221 gaaatccgtc ccttccagat tgagggaccg gctgaaatcc gcggggatat cagcgtccag
     2281 ctgcaaataa atcggttccg gggtcgcggt ggctgttggc gcactcgtgg tcgtggggtt
     2341 ggggagggta gcttccgttg gattcaagct tggttccggc tgcaggtttg ggcttgccag
     2401 ctcggcggag caggcacaaa gcagcgtcac gcaaagaagg atggaaaatg agtgaacaaa
     2461 aggtttggcg gacaatttca ttcgtttatt ggacctcaca gcaccagact tgaacattga
     2521 ttgtatccca accagccgca gcgggaatag gcctgggcaa tgtcggggca acacgttcag
     2581 gcaagtaaag aaaacctcac aaacaggcag caaaacggag cccggaaaac cgggccccgt
     2641 tgtttacatt tcctaacggc tgagccaggg caggtagatc aatgtctcct caatcggttc
     2701 tgcgacgcag ctctgcagtt tcacgtcatc caggtaccag ccttcagcgc tgccagaatt
     2761 cccgctgccg agccggaagc gcagcgatac ggtttgtccg gcgtaatcgc ccaggtccgc
     2821 cactgtccag acccaatccg aaacaccgca ccaggcagat ttgcctgcca atgggttata
     2881 cacgccgctt cttacggtac cgttgtaagg attggtgagc agcttcgagc tgctgagttg
     2941 tgaccaggtg gttccgccgt tggtgctcac ctccaggatg ccgccgtcat tgcaagccga
     3001 agagctgtca aaagtccagc ggtgccagaa tgaaagcgag agcggtaagt ccaccgcagg
     3061 caggtcgaag cccggcgaaa ccaggcgctg gtcggcaatc acagaaggaa ccgcgccccg
     3121 ccaggagctg acaggagagt gggctttcac tgtggagaga gcccagtggt acgctccagt
     3181 ggaagtgtcc gtccagccgg gagcgccgtc ttcgaaatca cccaggtaga gcgtctgagc
     3241 cgttgtgcca gccgggcaat ccccgggacc cgggcgggtt ctgaagctga aaacctgatt
     3301 gtcagtcgtc tcaccgcaag ggttttccgc tgaaacatgc cagtaataga gcgtgtcggt
     3361 ctgcagcgca gcaggcgggc tgtaagtcgt gccgctgatg ccggcctgtg tcagcacgac
     3421 ttgcgaaaag gcagcatcca gggccacttc cagccggtag ctgaccgcgc cgccgagcgc
     3481 ctgccacgcg aagccggtcc cgggagctat gttcactgcc ccgtctgccg gggagaccaa
     3541 ttgcggaccc gagtttaggg ggttgaaaac atttacaagc agatccgcct gatgggtgag
     3601 gctgccagaa gttcccgtga ccgcgacggt ctgcgatcca gaggaagcgc ttccattccc
     3661 ctcaagggtc agcacagcct ggccgggagg tgtgacaggc ccggcaggtt caaagctgag
     3721 ggaaaagccc ggcagttgac cgtttaccaa tgcgacaggc tcatcatact cgttcaagct
     3781 gcctacgttc acggttactt catgtgtgtc tgtgctgcat gtatccagcg tgttgggctg
     3841 cagggaaagc gtaaaatctg gcagcctggg gccaaggata atctccaggc tggggtcgcc
     3901 aaagatgtgg tattcctccc agtagtactg gtcccggttc gttgtgctgg catccaccgc
     3961 gaccaaaccc gcgtgaacca tttccctgac actgggaacg accgcgccgg tcggatccgc
     4021 gtacagcgaa ttaaaaatcg tgcgttcaag cgtgtcatcc tcgtaccaat aagaatagtt
     4081 gcttgccgcc gcgatggtca gcgcgccgta ctccggctca atcacccagg tatccgcgaa
     4141 actctcattc accgtaaaat ccgcggtcat gcaggcgtgc gaggcgacgt aaggcaccgc
     4201 gacgcccgta agactgcgga cctgggactg gctcaaggac ggtcccgccc agccggtctg
     4261 gctgccgtgc ccggtataaa tgaccatagc gcgttggtcg ttgatggagc tgatgacatt
     4321 cgccgaacct gctccgtacg tgatggcata cagcttatcg ccgccgggca tggggttgtt
     4381 cgggaaggtg ccggtgtacc ctttgggcag ggtgtaggta ttaattacgt aattctgggt
     4441 cgcctcggcg aagtcgtacc aacccgtatc gctcgtggca aggaaagccg ccttcttcac
     4501 ccaggcttcc tgcccgctgc gtccggcata ggtctgcagc ttggcaatca tatccgccag
     4561 ttgggctgtg cttcgcaccg gaaagcgccc gtagaagata tccggaacgt attcggtttc
     4621 gttatccatc gtgaaaaagt acaaatctgt gcgataggaa gacgattccg agcggaagga
     4681 gtagttcgta atgctgtccg cgccgttatt gtagtccccg accagcagca cataatctgg
     4741 cggggtcgcg cccagatatt ggctcttgat gtactgcttg atggcggtgg tcgtgttccc
     4801 gccgactgtc gtgatattcg ccacgctgac gttgaagccc tggctctgtt tcaggctcac
     4861 aaaagccgaa agtccgctct cataagcatc cgcggtgata atcagataat tttcagggtc
     4921 ctttgggtct gccaatggac gtccctggtt gaagttcagc acagttccgg cgtagatgga
     4981 attaaatccg ctggaattca gccgctcagc ctccgcatag gtagctgcca tatcgctgcc
     5041 ttccaatttc aacgcgaact caatgctgga gtatgtttcc aggccgccgc tggctggcgt
     5101 gtactgcacc ggcgcgatcg cgagctgcac gacgcggtgc ccgcgcacaa tatattcatc
     5161 gacgatgcgc accaattctc ccggataggc ttcctgcagc gcgtaaattt gcgagtcggg
     5221 cggaacctct ccctgagctt cggcgcattt agactgcgag ggttgacgcg gcgcgatgac
     5281 gccctccagc cccaggtcag caagcgaggt gaaacggctc ggcgcggaaa ggacttccac
     5341 gctgacatgc gcgccaaacg gcacttcaac atcgcgcagg atgaccggca gcgccggggc
     5401 tcctggctct gcgcgggttc caaaaccttc cccggtcagg ttcagatagg tttgcccgcc
     5461 aatttgaacc ggcagcaccc cgacgccgtc tgcgctggcg cgtaaggtta tgctttcagc
     5521 gtccgcttgc agcagcgcga cctcaggagc ctggcgcgtg tcgctgcttc caaacgcgag
     5581 ccaatccacc gggctggttt gggcgctcgc cgcgcgcgtc agcccgccca tcaagagcag
     5641 caggctcaag aaaataagaa tggttctgaa ataatccggt attcgtttat ggttcatagg
     5701 gggtcctttc atgggaagga aacttggaaa caacgaattt taccattccg gtatggaaat
     5761 ccgaagccaa gctggcgcaa atcccgactt cgtgcacagc cccgaaagcc ccgtgtaggt
     5821 tctattgctt tttcatatcc aaaacccgtg tgggaaaagc cttatccagg cagtcccggt
     5881 tctcctcttt ttgagaagta atatcgccta aatcttcttg tggcatttcg cgttgacttt
     5941 tcacctaatt ccctctaaac ttatgccatg ctcgcacacg tgttttcctg cgcggtaatc
     6001 ggtctggatg gggtcgtcat cgatattgag gtcgattttg ggcagggact gccgcatatg
     6061 gccatagtcg gattgcccga cgaagctgtg aaagaaagcc gtgagcgcgt ctattccgcc
     6121 attaaaaatg ccgacctggc ttatccccgc aaagcgctga ccgtcaacct cgcgcccgcc
     6181 tccgtacgca aggaagggcc ttccttcgac ctgcccatcg ccctgggcgt cttaattgcc
     6241 ggggagcaag tcagccagga acaaatcgaa ggcgcgttgg tcatcgggga gttatccctg
     6301 gacggctccg tgcggcatgt gcgcggcatg ttagcggtcg cggcggcagc caggcaagcc
     6361 ggtttcaaat ctctgttcgt acccgctgag gacgccccag aggcagccct tattccggat
     6421 atcaccgttt atccggtagc ctcattcgcc ggtctgtatc tgcacctgat cggcgaacag
     6481 cccctcacgc cggccgccgc ggtgcccttc agcccgcagt ctccccaacc catcgaaacc
     6541 gatttcagcg aaatcaaagg gcaagagcat gtcaagcgcg ctctggaagt agcggctgcc
     6601 ggcaaccata acatcttaat gtcggggccg cccggggcag gcaaaaccct gctggcccgc
     6661 gcgattaatt ccatcctgcc gcgcctgacg ctcgaagaag cgcttgaggt tacccgcatt
     6721 tactccattt gcgacatgct gccggcggat accccactga tgcaggcgcg tcccttccgc
     6781 gcgccgcacc acaccatcag ccatgccggg ttggttggcg ggggcaacct gccgcacccc
     6841 ggagaaattt cccttgcgca ccgcggcgtg ctctttctgg acgaactgcc agaattcggg
     6901 cagcgtatcc tggaagtttt acgccagccg attgaagaca aacgcgtcac catcagccgc
     6961 gccaacggca cgctcacctt tcctgccaac ttcatgctca tcggcgcgat gaacccctgt
     7021 ccctgcggct actacggcga taccctcaag cagtgcacct gcgcgcccgc ggccatcacc
     7081 acctatcaga aacgcatctc cggcccgttg ctggaccgga ttgacatgct tattcatgtt
     7141 ccccgcgtgg attatcaaaa gctcagcagt tcccggcagg gtgaaagctc cgccgccatc
     7201 cgggaacgcg tggaattggc gcggcagcgg caaagcgagc gcttcgcggg caccgcgctc
     7261 ttttccaacg cggatatgag cccggccgag ctgcgccgtt tttgcgccct ggaagcgcct
     7321 acccaggcgc tgatgcaaac cgccatgcgt caaatgcagc tcaccgcccg gggctaccac
     7381 cgcgtgctca aacttgcccg cactattgcc gacctggcgg gcagcgccga gattggtcaa
     7441 attcatctgg ctgaggcgct gcagtaccgc gccaaagagt ttgagttcta gtcagcgatt
     7501 tcaatctgcc tgcctgaaag cttgcttttc agggacggcc atcgacaaga gattaaccaa
     7561 ggtcacatca caaggagaaa atatgagaac aattcaactt ggaagctcca acatgcaagt
     7621 tccggtaatc ggcgtcggct gcatgcgcct gaaaacactg gaatcctccg cggctgaagc
     7681 ctttgtgcag tccgcgctcg ctctcggagc gaacttcttc gaccacgccg atatttacgg
     7741 cggtggaatt tgcgaggaaa tcttcgcaca agcagtccac atgagcccgt ccgtgcgtga
     7801 gaacctcatc ctgcagtcca aggttggcat ccgcaagggc atgtacgact tctcgaagga
     7861 gcacatcctt tcagcggtgg acggctgcct ggcacgcctg aaaacggaat atctggacgt
     7921 cctgctgctg caccgtcccg acgcgttggt ggagccggag gaagtggcac aggctttcga
     7981 cgagctggaa gcggcgggta aagtccgcca ttttggtgta tcgaaccata aacctatgca
     8041 aatcgcgctg ctgaaaaagt ttgtgcgcca gccgattctc gccaaccaac tgcagctcag
     8101 catcgcccac gccgggatga tttccaacgg tatccacgtc aacacgaacg aagacgcggc
     8161 gctggatcgg gacggcagcg tgctggatta ctgccggctg cacgacatta ccattcaacc
     8221 ctggtcgcct ttccagcacg acatgtttaa gggtgtgttt gttaacaatc ccgaatttcc
     8281 ggagctgaac gcctgtctcg accaactcgc gcggcaatat ggcgtaagca gcactaccat
     8341 tgcgctggcc tggctgctgc gccaccccgc caaaatgcag ccgatcgtcg gcacaatgaa
     8401 ccccgtcagg ttgaaggaat gcgtgcaggc cgcggatgtg cacctcagcc gggaagagtg
     8461 gtacaacatc tacagtgccg ccggctacag cctgccctga cgcccgacag ctgtttttca
     8521 tagaaatgaa gaaaaacgcc aggcgtgcat tcgtctggcg tttttggtaa tcggttttgc
     8581 tagctattct actggcgttt cctcagtggg aacggcagcg gaaggctctt catacaaggc
     8641 agcttcattt acctctcttt tttctcctgc ttcaggctga actgcctctc cgcttgcctg
     8701 accatcttgt ccggcggcgc cagttccttc agcctcatct tcgtcgtcat catcctcatt
     8761 gtcatcctct tgtgctttgg gtgcagcata aatcctgcgg gcagccggca acagaaggag
     8821 aatgattaca ccagccgcca acaggatctt tccaatggga ataataaggt tcaacccgcc
     8881 aaaagccccg aagagtccaa gtactcctga aatcagcagc agcgccgcaa tgatgattcc
     8941 caggatgacg ccccactttc tggctttaaa gataccaacc gcagccgcga tggcacccaa
     9001 cagcgtcagc acgtacgcgc caaggctcaa ccaacgcgag acgccaaagc ctctgcgacc
     9061 gggcacaccc cctgggaagc cgctgctatc cgggcgctgc ccattgaagt catcccgatt
     9121 gaagttccct tccggaggag tttgcccttc cccgggtgtg aaatcgcctt gcggcggggt
     9181 taattgaccg ttaccgctcc cgttcgaagg cagagcgccc atcggtccag ctccgcctgc
     9241 aaaattcatc ctgaaagtgg tgtaggcggc aaatccagcg ctcagcaaag ccagcgcaag
     9301 caggcaaacg cctgcaatga tcaatacagt cttttttgta ggtttcatag ttctcctaaa
     9361 ctattcgtag cgtaacgctt caacaggccg caaacctgct gcctggaaag ctggatataa
     9421 tccgaacacg aggccaacca gagttgccat cgcgaccgaa aggaatacaa cgttcggggt
     9481 gactgtagcg gtcagcaagc cgctggccga aacgccccag gcaattgccc agcccaataa
     9541 aaccccgagc aagcccccca tcaaagaaag cgccagtgtt tcaaccaaaa actggaagag
     9601 aataacgccg cgcgtcgcgc cgactgcttt ccgcaagccg atttcgcgcg tacgctcccg
     9661 tacagatacc agcgtaatat tcataatgcc aattcctccg accagcagag aaatgccggc
     9721 aatggcgccc aatagcgtgg tcagggtact gctgatgctg ctgagggatt caagcatctg
     9781 ggactggctg ctgacattaa agggcagctc atcggtcagc ttgttcccgt gttcccgccg
     9841 caggatgaat tcgatacgcg cgatgagggt gtccacctgt tccgcatcgt aggcggaaag
     9901 tgagatagag gacaaggtgt actgtccctt actccgggcc tccgctccaa acaccaggct
     9961 gtatccggtt tgaagcggga tgagcaccag gtcgtcgttg ctgccttttt ccgccaacac
    10021 ccccaccacc tcaaagctct tcccgttgat ttttatctcg cgcttgaggg ggttgagacc
    10081 cgcaaacagg tcctccgccg agctggaacc gagcacaact acccgttgct tttgaaaatt
    10141 atcctccgcg ctgatgaagc gcccttttgc cagttcgtag gcattaacat cgatatattc
    10201 aggcgtgacc ccacgcacag atgcgtttgc gctgcgatcg ccgttcatca cgctggcaga
    10261 gccctgataa tacggcgcta tctccccgct cgcgcctaag tcctccatca gcgcctcgaa
    10321 atcgctgtat aacaactgct gcgactgacc gccgtacata aactggaagg agcggccggt
    10381 cgaaatcgtt atcagattgg tgcccatacc ttccacccga ctggtgatac cgctggtagc
    10441 gccctgcccc gctgccatca gagcaaccac ggccgcaaca ccgattatga tgcccagcat
    10501 ggtcaagcct gaacgcagct tgttggcgct gatagcccgg aacgcaattc tgaaggtctc
    10561 aaagatactc atgcgtcctc ctcctcatcc gaattgccct cccaggactt aaccatcagg
    10621 tcatcggggc gcggcatgcc ggcttcgatt ggttgaagaa ctgcttcatc actgacaatt
    10681 ctgccgtcca ccaactggat aatccggttt gtgtggcgag cgatgaaagg gtcatgcgtt
    10741 acatacacca cggtctgccc ttgttgtcgg ttcaactttt gaaaaatctg catcacctca
    10801 tccccacgac gggtgtccaa attgccggtc ggttcatcgg ccagcaaaat cgtgggctta
    10861 ttgacaagcg cccgggcaat ggccacccgc tgctgctcac cgccggaaag ttggttcggg
    10921 tgactgtagg cgcgctcgga taaaccaact atcgcgagcg cttccagcgc gttttgccgg
    10981 cggacttttg gtctcactcc actgtaatac atgggcagca tcacattctc gatagcagtc
    11041 gtgcgcggaa gcagattgaa ctgctgaaaa acaaacccga ttttattgcg ccggatggcg
    11101 gccagttggt tttcgctaag actttccacc ggctgaccgt caatcaggta gacgcctttc
    11161 gtgggcacat ccagacagcc aagaatggac atcagggtgc ttttcccgga accggaaggc
    11221 ccgataattg ccagcatctc gccgtcctca attttcaggt tcactttgcg cagcgcgcgc
    11281 acttttgtat cgcccaagcg ataagttttg gatacttcct gcgtctcaat catcctgagc
    11341 ctctatggat tttgtggcgc gctgccgctg ggaggctgct gaccgccctg gggcatttca
    11401 ccgccgccgg aaggtatctc tccgcggttg ccgccccctc ccaggatgcc taaacctgcg
    11461 ccggagcttt cctctgtcga ggagacttca ataatcagca ggacgaaatc ccccacggta
    11521 aggtcgccgc taacgaccac tgtgtcgtcc gacagaatgg tcccactgac gacgtccaca
    11581 cgctcgtagc tgccgttccg gatgacctgg acaaactcgc cggcatcatc gctctgaacc
    11641 gcgctggcag gcacaaggag tttggtttcc ggttccccaa tctgaatgga gaccgaagcg
    11701 ctcgcgttga ttggaatggc gtcgttggtc ttgtcgagct ctacttccac ttcgtaatac
    11761 accatgccgt tggaaactgt tccgaccgga tcaatgctcc aaatatgccc gctgaggggg
    11821 agctcaggga gaatatcgac cgtaaccgtt gcaggcaggc cgactgaaac ttgtgtaacc
    11881 tcactttctt ccagcgagat cccgacatac agcgcggaac ggtcaatcag tttgacgctg
    11941 gtgtggttat tcgaaaccag gattccaggt tcatcataaa tcgcggcgac ggacccgtcg
    12001 aagggagccc gcaggttgat cccgtccaat gtttccaatg cctggcggta cgtttggatg
    12061 gaagcgctgg tcgcgtcatc cggaatgtcg ccgccggaga tgagtgtcag gtaattctgg
    12121 gcttcagtta acgcgctgcc tgtaacaagt accgcgttgt cgtattcact gtacgtttgt
    12181 gccgaagatt gtgacttata cagatcgtac aggtattccg ctgtctccat ttcggttttg
    12241 gcctgataga gagcggtgta tgcggtcgca aggtcggtat cgtaatggtc gctgtagtct
    12301 tcgtcttccc agttctggta gttttcgtaa gcgtcgtcgt actttgcctt ggccaggatg
    12361 tattcagcat gggcgttctc aatcacatcc tcgtcctggt aatccaggta tttccggttt
    12421 tgaaccgcag tgtcataggc ggactgcgcc tccaggactt tttgctgggc tgccgccaca
    12481 gcagagggag aagtgaactc cgccagctgt tcttgcgcct ttttcaggct ctccgggagg
    12541 gaactggtat ccaaggtcat cagaatgtcg ccacgcgcga cttgctgccc tactaccacc
    12601 ggcaccgttg cgattgtgcc gctgagcgtc cagttcacag tcgcaatctg ccggggcgta
    12661 acggaacccg aggcttcaac agtattggtc agggtgattt ctgtcacctg gccaatatca
    12721 ttggtggtcg tgctggcaga ggaagcgctg ttggcgcagc ccgaaaccaa tcccgcgccg
    12781 gttagaaaaa ccaaaaagta aataatgccg tgttttttca tttcagatat ccctttccaa
    12841 tcaaagtcat ccgcaggcac tgctcatttt cacgtttatg cagcacgaaa gttctccaca
    12901 aactggactt caattatttc atgaaagatt ctatggattg tctggttctg ctggtatagg
    12961 actcgtatag acgcatcaac tcttcttcgg gcaagcaaat gcgaaataaa actgcatcaa
    13021 gccgcttttt aatcataggt ggtcctgcac cgtacagatt gatttccata gcgggaaaag
    13081 atattgagcc tcggtttttc cgcattcaca atccatgccg ggatagtagt atggtaagat
    13141 tgtgtcagtt tcagccattg tttttacaac ttaagaagga gattcctatg cgcaagacgc
    13201 tacctatgct gtgtatattt atgggtttca ccttatccgc ctgttttccg cagacagccc
    13261 aggagccgca agtggattat tccccgaccc tgagctccta cgccgtagaa gtcaacagct
    13321 tacagcgtga cattcaggac ctggaagccg ataaacaggc gctcacaacc gaaaaagaag
    13381 ctcttgtagc ccagattacg cagctgcagt cgcaggccgc ccaatccgcg gagtcggccg
    13441 cacgtatcac cgagttggag ggtcaggtag ataatttgca gagccagttg aacgatgctc
    13501 aggccggcct gaacggtttt ggcgcgatgg ttgccggata cgaggaaatc cttatggggg
    13561 agtactacga tgaagacgga ctgatggtcc tatgccccga cgccttcgat gtctcctttg
    13621 gctacgtgga ccgcgtttcc atgcggaagc agttggtaga ttatatctat aaccaatacg
    13681 tcaatgacgg gtacactgta gatatcaata cgattacatc cgaacaccag caaatctgga
    13741 cggatgaaca ggatgcgctg gtcaagatcc atattctgga tttcctggag ccctttatgg
    13801 ttacttttga aaaccctgaa tatggcattt acagcgcggt ttattctgtg cgctggcagt
    13861 gtttcgtcga ccatccggtt ctggaagcgc gttatcttgc cctgcacgga aatgaataac
    13921 gccgcttgat aggaaaaaag gcacagagat gcctttttct gtgcctgtta gcgcctgcat
    13981 gcctgccttt tcccgccagg catttttctc cattcctccc aaccaaaaaa gcagcctctt
    14041 gcccggattc ttcaggtttc caactcgttt ataatggcgg catggcaagt accgtttttg
    14101 gacgcaaata ccactggcag ctgcgcgagc agataggaaa aggggatgct ggcgaagttc
    14161 tgcgcgtgca aactgaactc gggcgggagc gcgggctgat gaagcgcccg gtgcagaacg
    14221 tttccggcgg caccatcctg cgccaggccg tccagattga gaacgaaggc aaagtgctgg
    14281 cagccttgaa cgggctcgac gtgcgccgca acaatctgac cgtgcacaca ccgctgctcc
    14341 tggaccaaag ccctgagggg acttccaaaa cagcggctct ctttatcgta agcgaagaag
    14401 tgcgcggaat tgcgatttcc aacctgctga agaatctgct ccagaagaac gcctccttct
    14461 cgcagacttt ggtcctgaaa gtgctctcca gtctgtttac actgctttcg cgggtacacg
    14521 cgctgggtat tgcctggaat gacgtcaaga tggaacacat cttttgggat gagaccacga
    14581 atacgctctc attcatcgac tggggcaacg gcttgttttt tgaccccgac gaagcgcagc
    14641 acccgcagca cccggtaagg ctggactacc aacagttcgt cagcgagggc agcctgctgc
    14701 tgaaccagat tgccccggat cttatttccg aaattgactg gccgcaggag gacgcgaacc
    14761 tcacgcctga ggcgctgctg cagctgcagc accgcgtgga gtacatggaa gcgcacctct
    14821 ccatgcgcat caaggagtac caactggcat ttgataaaag ttcccgacag attaacagcc
    14881 tggcggaact gcaggacatt cttgagctca agcaggcgct gggcaggctg ggcgtgcagg
    14941 tacaaaccca gaccgccctg cagtccgtac ggcgcttcgc cctcagcctc gcgcaggcaa
    15001 acgcctaccc ggaagtccac gcgctggtcg aactgctgcg cacttacctg ccggaagacg
    15061 caagccaaag ctggcagttg gtggattacc tgctcagcct ggaggacgcc tgggaccagc
    15121 cggcttttcc ggtcgtactt gaaaaaacca tcgccaacga cctgcccgct gcgatttggg
    15181 aattggaagg cgcccgggag agctccggga accttccgcg cctgatcaac atccaattgg
    15241 ctatgcgcag cgcggccggc gtgccggatg ccctgcgcga tacgctgctg gagcagctgc
    15301 gcgcctggct ggatgacaca tacgcgcgcc tgctgcacac ccgccaggaa ttcccggctg
    15361 agatagacgc agccgccgcc ctggaaactt ttgcctccaa tctggatgcg ctcctctcca
    15421 actggcagaa tcttggcgcc ggcgaaatgc tgggagataa gctcctgctc ctgcgcgacc
    15481 tgctgcgctc acctggcgcg cacctgacga acctgcccca atccctgtcc caggcgctta
    15541 acctggaact tgccaaaacc cgcgaaatcc tgaccttctg gaccgaaggc gagctaaata
    15601 aaagccggga cgccctgaag aatttcttcc tgctggagcc gacgatgcct tacttacggg
    15661 agatttccgc aggcatcgaa gccttgaaag gctggatgga cgagctttcg cgcggtcctc
    15721 aaccctggca aagcgtaaac gcattcgggc gcggtctgct ggaacgcaaa ccggacctgg
    15781 ctaagttcct gagccaaccc gcctggcttg ccagcctggt gcagtgtgcc gaagccttgg
    15841 ctgcggcctc ggacggggaa atgctgcgcc tgctggcaga gaataatcag tggcccatcc
    15901 cctgggtaga ctatggtgaa atcaccatcg aattccgcac agccgcggct tccaccagaa
    15961 tgaacgccgc ccagaaagat gcgcttgctg gctttcacca ggccttgtgc ggggaccagg
    16021 acttgccgca ggcgctatcc cggctaaagt cgcttctgcc tgggctttac ggtcagtacc
    16081 gcctcttagc ccagggcttt gaaaccctct tcactccgct tcccgcccaa actcaggcgg
    16141 tggaagcggc agccttcccc cccgcggaca ggcaaaacgt cgttcaggcg ctggaagttc
    16201 ttgactacct gaagcgctgg aaagaatccc agctcgacgc gggcacagca atgggcgcgg
    16261 agccgccagc tgcttttcag ggctggcatc tggtcacagc cgcgcacaac gcccagagaa
    16321 cctggcacgc cgcgctgcag cccgccctga ccctcctgaa acaaatgcgc tgggaggcgt
    16381 tggctgaatt cacgcgcgcc aacaagctgc cagacgccct aacagcctgc gtgcaagccc
    16441 ttgctcagct tgtagcggat tggcgccgcc tgcaggaaac gggcgtcttc ccggagaatg
    16501 tacgcgagat gattagcctc gcggaacaag cccagaccca attctttgtc ttctggcagg
    16561 agcaggaaca agccccctcc cgcccactgc gctggttgat ccaaatgaac caatccgcct
    16621 tcagcggaat ccaccagtcg ctgcaccagc tgacgcgcca ccttcagacg ctggaacgca
    16681 cgctgctcgc gctcgcccgg cccggggttg cccgctcgcg tctggcctgg aacggcgccg
    16741 gcgatatgat gttcgccctg ggtcaaattt ccgccctgct ggaaccgcct tcgcgtaagc
    16801 cctcccgggt ccgcctgtgg caggagcagt acaatcaggt gctgaaacaa accgagtgga
    16861 aaaacgccgc cagcctgctg gaaacgcttg accccgcgca cccgctggca gcctggttcc
    16921 aggagctggc atccaaggat cttgatatct tccacgaaac acccagacaa aaatggtaaa
    16981 atagccggaa tttacggaca aaatgataga gaaaaaacta accccccaaa acggattcta
    17041 tctggctgag ccgctggaca cctggctgac agccggaaac cgcgattacg ccattcccga
    17101 agccgccggc tcgagcgcgc gcgtcttttt actgaaggac gagtcgaaca gcggcggcta
    17161 caaacgcgac ccggccttca aagtgatgcg ctatgacaag atttcttatg ccaggccgct
    17221 gtttctggag gagtacaaaa tcctggccgg tctgaacggc attcaggggc tcacttccat
    17281 gctgcagggc ggcttcctga aacctgaccc gggctgcgtt tggccctcgg aattggcgcc
    17341 gctctccaaa gacgcggagc aaaatgccag cgcgctgcag gtgagcggaa agctggcaat
    17401 cttcaaaccg gaagaaaccg acgacgtgct gcagcaaatg gacgaacgcc tcgcggatgg
    17461 atggctgccc tttcttatcc tggaacgccg ctgggaagac aacctctata tgctctgcga
    17521 cgccggctat acccgcggga attttgccaa aaacctgacc atgaagcagg tactggatat
    17581 ttccatccag atttgcgata ttctcgccca ggctcatcag cgcggagcaa cttttttgga
    17641 ccataagctg ctgcattact actggaatga ataccgacag aaagtgatca tgatcgattg
    17701 gaacatcggt cactggcaac cggaaaacat acagcctgaa gtgctgcagt tcgacctggt
    17761 acagttcagt tcacgcgccc tgcatcacat cttcaccggc cgtcaggcgc cgggctcggt
    17821 cgctgtcggc cccaaccgcc ccgaagatat tgccagcgcg cccaggcact tcaaagcttc
    17881 gtattcctat gacgttcaaa atcgtttgaa tcaggaagaa atgcgttttc tggagaaagc
    17941 cctggacggc gcttttttga gcgccgcgga gatgcgcgag catctgcagt tccttgccag
    18001 caaacggcta taatcagctt accttattga aagcctgaaa ggagcctcca atgattctcg
    18061 acctcattga acaggccaaa gctttactga aaaagaataa tccggcagat gacgaactgc
    18121 tgcaggtaga aaacgcactc tcgacctatt tggaagcagt ggccacccac cgcatctcgg
    18181 atcaggtgga cctggatgac attgacgaag ccaaccgcct gctgcgggaa attcgccgcg
    18241 aacgcggacg gcggattgcc agtcaggcag agcctgcgca aacggaaacc tcaacgtcca
    18301 cctctaagcc tgctgcggac caacccccaa tgccaaaacc tgcggtcaac cggttggacc
    18361 tgcagagcga cgccggcatt gacccgctgc gcaagttctt ccagtccagc cacgacccgg
    18421 aagccgaacg catgatggac gaagccgaag agctctttta taaaggcaat taccaggctg
    18481 ccatcccgct ctacgaaaaa gtcatgcaga tggaacccgg atggtcccgg gcggaagagc
    18541 accactcgga agccgaggag ttcctgcgct caggcaatat cccatcagtc gcgctgcccc
    18601 ccgaagccgg caaagcctac ggcaaagccc aaagcgcggc gcgcgtcttc cgctaccagg
    18661 tagcgctgaa ctacctggat gaagcctttg accacctgca agctgccggc atcaaacgct
    18721 ggcgcgaagg ggaagaactg cgccacgacc tggaaaacca gatgcaggca tatgacgtat
    18781 ataaagaagg cctgaatctg ctttcgcaag gggaactggt gcaggcgctt ggcaaaatcc
    18841 agactgctgc cagcgcggtc gcgatcccgg agtatctgga taaggcctcg gaagtacgcg
    18901 ccgatatttc gctgctcgaa gaggtcagtg atatcgtcag cctgagcggc aagattccgc
    18961 ccggcaaatt ggcggatgcc aagagcaagc ttgaacggct gcgcatgaag tacggcagca
    19021 tctcccagat tagccgtctg caaaaccgcc tggatttgct gattccagcc accatccagt
    19081 cgctgctgga taatacccgg cgcttgagta aagaagccgc tgaagcgccc acgctggcca
    19141 acgcgctgca gaaaattgct ggcgcgcgtg aaaacctgga ccttctgcgc cagctcgatg
    19201 cctacgattc ccaaagcctg ctgctggaga atgaaatcag cagcctcgag tcggagattg
    19261 aagccaacca ggatgctatt aaccgcgcgt tggaagccct gaaaagcggc agccgcttct
    19321 tcgcactgaa cgccttcctc atcagtcgcg gcgtacgcaa gcgtttccca aacgaccccc
    19381 aggtcatcac gctcaaacgc ggcctgtcgc tcagctatct ggtggcaggt ctgggcgggc
    19441 ttttggcaat cctcgtgctt gggctgctca tcagtgtggc tacgcgcagc atcagcagtt
    19501 ccatccaggc gcgcaatctg gcccgcaccc ccaccgccac ctttacccct acaatcaccc
    19561 tgacccctac gattaccccg acacccaccc agacgcttac ccccacgccg gattactcgc
    19621 ctaccccaac caaaaccgca acgccgaccc ccgtaatggt tgtggaaacc gtccggcagg
    19681 tatatgcccg caacgactgt tacgaaggct atatcgccac cggccgcatc ccacaaggga
    19741 ctttggtcac gctgctgccc atgccggagc gccgcttcga caatttcaac cgcgagtgtc
    19801 tgctggtgga attccgcggg aacggctttt cgatcattgg gtacgtcctg ctcgtggatg
    19861 tcatcgccaa gtaagccggg atccttctga ccgtcgaacc cggtgtttct tgaaggaaag
    19921 ccgggtcgtc gttttcacgg ggatataata aagacgcgga caggtgacga tggaaaatct
    19981 ggaacaggca tatcaggaat cgctggattt catttacagt ttcatcgatt tcagcatgaa
    20041 acggcacctg gacgacagcc accgcttttt caagctggac cgtatgcgcc ggatgatgga
    20101 tttgttgggc agcccgcagg aaaattttct gagcatccac gtggcaggca ccaaagggaa
    20161 aggctctacc gcttccttgt gcgcgtccgc gctgcgcgct gcgggtttca aagtcgggct
    20221 gtatacctct ccccatctgc aggaatttac cgagcgtatc caagtcgacg gcgcccggat
    20281 cgaaaagcag gcgcttgttg acctggtcgc gcgcattcgt ccgctgaccg cgcaagtgcc
    20341 ggagataacc agctttgagc tgatgaccgc gatggcgttt cagcactttt ctgaacgccg
    20401 ggtgaacatt gccgtggtgg aagtgggctt gggcggccgt ttggacgcta ccaacatcct
    20461 cactcccctg gtttccgtca tcactcccat ttcttacgac cacaccgcca tcctcggcag
    20521 caccttgact gaaattgcct acgaaaaagg cggcatcatc aaacccggcg tcccggtggt
    20581 gctctccccc caaaacccgg aagcagaaca ggaactggcg cgcattgcgg cggaaagggg
    20641 ttcgcctctc acgcgcgtgg agcaggatta cgcgctgctc atcctcaacc attcgctgcg
    20701 cgggcaggaa cttgccatca cagccgggcc ttccagccca tcccgggcgc actccctggc
    20761 aaccgcgccg ctaaacctgc atttgccctt attaggcgcg caccaggcgc aaaacgccct
    20821 cacagcgctt ggcgggctgg accaagcgcg cgtgcagggc ttggctctct cccgggagga
    20881 cgtccgcaaa gggttcgggc aagtggaatg gcccgcgcgc ttcgaaatcc tgcgggaaaa
    20941 cccgcctgtt gtcatcgact ccgcgcacaa cggcgattcg atgaaccgcc tgcggcaaag
    21001 tctggacgat tatttccccg ggcagaaatt cctgctggtc tttggcgcgt ccgcggataa
    21061 agaactggac gcgatgctga aggcgatcct cccgcgtgtg gagcgcgtca ttaccaccca
    21121 gtccacccat ccgcgcgcag cggacccagt ccacctgatg gagcagatcc gcccgtacgg
    21181 tttaccggta gaagcgtgca atcccgcaga ggatgcattg gcgcgcgcgt tggaattggc
    21241 tggcacggac tgcgggatta tcgtggctgg cagcgttttt atcgccgcag ccgcacggga
    21301 tatctggttt tccagcagca atggcagagt cgcggtattg cgggtataat ttttacccct
    21361 gaaagtaaaa tccgaggagt aagttttgtg tatcaagatc ctttcttttt taaccatgta
    21421 aaccgggacg atccaattca gatttcccgc tttgcgacac tgagtaatct tcgcggcgtg
    21481 aaagacagaa agccggacaa agacggttat ctggtcgggc ctgcgattga cctggaggaa
    21541 atgctgctgc tgccgcacgc catgatgcct ctgacgttgg cagaggatcc gaacgctatg
    21601 gacacgctgc gtgatgccta tcaaaatagc gaaactctct ttgcgtgctt ttccaggcag
    21661 gatttcaacc acctgacaga aacggcagaa ccagagaaac gctatcttga aatcgggctg
    21721 gaggtcgcgg tcggaagcct cattagtttg gataacgacc gcgagagtgt gctgctgcaa
    21781 gcccgaaacc gcgtcaaaat tgaggatatc tggcaggaag acggcctgtt gatgatgcgc
    21841 tgtctgcctg tcaaatcgcg ccagcgcaag tccaactcgc tgaccgcgct aaagagggct
    21901 gtggtcaacg agttcgagcg gtacgccagc ctggtggaat ttatcaacga tgatgtggtg
    21961 gagtttgtca gaagcgtgga agatcccggc gacctggcgg atatggtgct ctccacgctg
    22021 gacctttcgg aggcagaccg tctggccttc ctgatcgaaa ccgacggcgc cgcacgcctc
    22081 gaagcttctt tgcgcaacct gaagcatgag gtccggctgc aggaattgga agctgaaatc
    22141 caaaaccgcg tccaatcctc tttggaccaa aaccagcgcg aaaactacct ccgggagcag
    22201 cttaccacgc tccagcggga actgaatgac ggcaagccga tagatcccga gtatcaggaa
    22261 ttgagtgaag cgctggccaa aatctctctg ccgcaagagg cacgggaaac agtcaataag
    22321 gaactggacc ggctggctaa cacccccccg ttttcccccg aaaacgcgct tatcagtgcg
    22381 tatttacatt gggtggcgga acttccctgg gataaagaga ccgcggacaa tctcgacatc
    22441 aaccacgccc gggaaatcct ggagaagaac cactttggcc tgcaaaaagc caaagaccgc
    22501 attctggaat atttggcggt gcgcagcctg aagccaaaac gcaatcggca gcccatcctg
    22561 tgttttgtcg gcccaccagg gacaggcaaa acctcactgg gaaaatccat cgccgacgcg
    22621 ttggaacgca actttgtgcg ggtctcgctg ggcggcgtgc atgacgaagc agaaatccgc
    22681 gggcatcggc gcacctacat tggcgcgctg cctgggcgca tcctgcaaac catgcgcaaa
    22741 gccaaatccg tcaatcccgt cttcatgctg gatgaaattg ataaactcaa cgctgatttt
    22801 cagggagatc ctgccgcggc tttgctggaa gtcctggatc cggaacaaaa ttacgctttc
    22861 tccgaccact acctcgaggt agcttacgac ctttcgaagg tcctcttcat caccacagcc
    22921 aacaccgtct ccagtatccc ccccgcgcta ttagaccgca tggagttgat tgaatttccc
    22981 ggctacatcc tggaagaaaa aatcgcgatt gccaatcagt tcctcatccc caagcagatg
    23041 gaagaaaaca gtctggatgg ggagccaatc ctgttccagg aggaagcgtt aaaagctctg
    23101 gtggaaggct acacctacga agccggcgtg cgcaacctcg agcgcgaaat cggttctgtt
    23161 ctgcgcaaga ttgcccggat gaaatcagaa ggcaaaactc tgcctgctga aatcagcccg
    23221 gagcttgtgg aggaactgct cggcccggcg gaattcttcc cggctgtggc ggaacaaaac
    23281 gatgaaatcg gcatggccac tgccatcgcc tggactgaaa acggcggtga gattatgccc
    23341 gtggaagttt tggtcatgga gggcaaaggc aacttgcaga tgaccgggca aatcggtgaa
    23401 atcatgcaag agtccgcgca agccgcgctc tcctacctta aatccaaaca ggaagctttc
    23461 cagatcccgg ccgatttttt tgaggacatg gacatccata ttcacgtccc ggaaggctcc
    23521 attcccaagg acggccccag cgcgggcatc acactggcaa ctgctctcac ctccgctgtg
    23581 ctcgccgtcc cggtgcggca cgacgtagcc atgaccggcg agatcactct gcgcgggcgg
    23641 gtgcttcccg tcggcggggt gcgcgaaaaa gtcctggcgg ccaatcgttc ggggatccgt
    23701 accgttttgc tgccgcgcaa gaacgagaag gacctgcgcg aagtgccccc acaagtattg
    23761 aagaagctcc aaattgtgct ggtggagcat atggatgaag ttctggcggc tgccctggtc
    23821 gataaaccca tcagccgcaa accccgcgcc gcatcctccc gcgccaaaaa gaaaaaaagc
    23881 gcgtctgacc agcaggacgc gccgcaagca tcagaaacaa gctgctaatc acacttcagg
    23941 cggagctgtt cggtcctctg cctgaagtgc cctaatcccg ttttgcacct gtgctaagat
    24001 gcggttcatt tcagcttcca gttttgtcag cgtttccacc acgtagcggt cggcgtcgag
    24061 ttgggtttgg ccggcttcgc ggcgggcctg ctcggtaatt tgttctgctt ttgcctgcgc
    24121 cgccagatag acatcgcttt tctcgatcat cttctcgctt ttttcacgcg ccagcgccag
    24181 cgtgcggttg gcttcttcct gtgcctgcgc caggatgcgg tccttctggg cgataatctg
    24241 ctgagacttc ttgatgtcgt cgggaatgga tatgcgcatt tggtcgatga tatccataaa
    24301 agcattttca tccacaacta cgctccggct cagcgggatc ggcctgctgt tgttgaacaa
    24361 ttcttccagg cggtctacca gatgaaggat atccatttct gtctccgtgt gtgctgaacc
    24421 atgcgtgtta attcagttta tcatgaattg ggtgcggatg cgcacctttt ttcaggcacg
    24481 ttttaggcgg aacaaatatg ttcaagccgc ggcgctccga acg
//
LOCUS       DAOQAD010000019        24240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_2081_length_24240_cov_6.780980, whole genome shotgun sequence.
ACCESSION   DAOQAD010000019 DAOQAD010000000
VERSION     DAOQAD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24240
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2081_length_24240_cov_6.780980"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..930
                     /locus_tag="PKL60_02910"
     CDS             388..930
                     /locus_tag="PKL60_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNW95117.1"
                     /translation="MEAELSAEENSRTLDLLTQIEANPDISQASMADALGVAVGTINW
                     YLKRLISKGYVKVKRAQRKKLRYIITPEGIALRASLTVDYIRTSFDLYRRVRERSAKC
                     IEELRQHGCDSVRILGDGEVAEVIRLTCIEHGVELTEAEGAPEIHIQGIKLALVFPDA
                     EAACPPAQLIVYRGNYGTGN"
     gene            914..1861
                     /locus_tag="PKL60_02915"
     CDS             914..1861
                     /locus_tag="PKL60_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNW95118.1"
                     /translation="MEQEINFSGKRICVTGGAGFLGTHLIANLRTKGAQDIFIPKYED
                     YDLVKPDDIARMLEDSAPDILIHLAAHVGGIGANMAKPAEFFYDNLMMGVQLMHQAWQ
                     RGVEKFVAIGTICAYPKFTPIPFKEEHLWDGYPEETNAPYGLAKKMLLVQAQAYRQQY
                     GYNAIFLLPVNLYGPGDNFNPASSHVIPALIRKCLEAKDQGASEIVAWGDGSPTREFI
                     YVEDAAEGIALATQRYNGAEPVNIGSSFEISIRDLTELIARLTGFDGAIRWDTSKPNG
                     QPRRKLDTNRAREYFGFSAKTDFEEGLRRTIAWYQDHRS"
     gene            1867..2706
                     /locus_tag="PKL60_02920"
     CDS             1867..2706
                     /locus_tag="PKL60_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95119.1"
                     /translation="MSETPVSAKMVVLRPSKGWLGIDLKELWFYRELIYFLTWRDIKV
                     RYKQAALGIAWAVLQPVLTTAITSVVFGVLLKVESDGLPYPVFVLAALLPWHLFQLSL
                     QKSSISLVGNANLITKVYFPRVIIPLSSVLAVMVDFAISLVLLFIAMAIFHMPITLNA
                     LWLIPLTLLAVLAALSVGLWLSALNVQYRDIQQIVPFLVQIWMYASPIVYPISTIPEG
                     TLRSLYSLNPMVGVVQGFRWALFGGTPPDFTLIISSAVVVLLLISGLFFFRRMEKTFA
                     DVV"
     gene            2706..3989
                     /locus_tag="PKL60_02925"
     CDS             2706..3989
                     /locus_tag="PKL60_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95120.1"
                     /translation="MNASEYSIQVNGISKRYKIGTAQQRADTLRDLITNQVQRIGQSF
                     SRGGRANKSADTIWALKDVTFDVRQGQALGIIGRNGAGKSTLLKILSRVTDPTEGYGE
                     LRGRVGSLLEVGTGFHPELTGRENIFLNGAILGMTRKEILTRFDEIVDFSEVGQFIDT
                     PVKRYSSGMYLRLAFAVAAHLEPEILVVDEVLAVGDADFQRKCLGKMGDVAQQGRTVL
                     FVSHNMSSILRLTEETLVLEKGKVLLRAPSTEAVDFYLNRGLSKLGERFWEEDEVPAS
                     AAPFRPLAIRVRAKDGAVSDTVRSVEPFTIEIEYTLSEDLTGLRVGFYLMSTRGEPIF
                     TSFDTDSSELFQAYPTRAKGAYTSRCVIPANTLNEGRFVLGINASSYHIRRYFQDEQA
                     LTFSVDASGAPGTHWPEPRPGGVRPVLDWQIAREE"
     gene            4331..5809
                     /locus_tag="PKL60_02930"
     CDS             4331..5809
                     /locus_tag="PKL60_02930"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95121.1"
                     /translation="MTDHQPKKICLFASLHYWIEESALIGLALAGQGHSVNLAYLPYA
                     EWDKEITPFDLRRQDLYTREVFAEAKNFLRLSPLADEKVSPERLPAEALRAVEQVSDY
                     DTQYTLQVEETDRGSALYQLRYARNLQAAGALYAWLRAEKPDVLIVPNGTILEMGVAY
                     RVAQLLGLRVVTFEFADQRERIWLAQDDEIMSHDTNALWQALGGQSLPEAARRAMLNL
                     YAARRNARLWGNFARQWQQTPLQGAREVRDRLGLDRRPVALLATNVLGDSLTLGRQRI
                     SATMADWIVGTLAYYTRHPEAQLLVRIHPGELLTHGTSMVEVIHAKFPQLPENIHIIL
                     PGDKTNTYDLVEIADFGLVYTTTVGMEMAMAGLPVIVAGKTHYAGKGFTLDPESWEEF
                     EDLLSRLLADPAAFRLNPRQVEQAWLYAYLFFFEFSLPFPWHLLWLADDFSARPLRFV
                     LSPEGQARFAPTFGYLAGEPLDWAARGLARLNELPAETEESA"
     gene            5806..6663
                     /locus_tag="PKL60_02935"
     CDS             5806..6663
                     /locus_tag="PKL60_02935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW95122.1"
                     /translation="MTQHDVKQEIGRFYNEVGWQLEQDGNFQNAQYEDLRPVSAEYIA
                     RAHARVARHLPKSGRYLLDAGSGPVQYDAYLQYSAGYERRVCLDLSFVALKQARARLG
                     ARGWYVVADIANLPFKPEAFDGIVSLHTIHHLPTDEKPGCYMGLYERLTPGGSMVTVD
                     GWSEHKLRKTWTWLIRLAEKLRRQKSPGQEEGARAAAESKPAGTYVVKNNAAWFKAAV
                     GSQLPARIFTWRSVSVHFLRTVIQPEWGGKFWLKVLYRLEECFPRYFGENGQYPLIVI
                     SKPNKDAPA"
     gene            6676..7683
                     /gene="pseB"
                     /locus_tag="PKL60_02940"
     CDS             6676..7683
                     /gene="pseB"
                     /locus_tag="PKL60_02940"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HNW95123.1"
                     /translation="MEWNNKVVLVTGGTGSFGKKLIKILVEEYHPVKIIVFSRDELKQ
                     HEMRVSGYNHPSIRYFIGDVRDRERLSRAFYGVNIVVHAAALKQVPACEYNPMEAIKT
                     NILGSSNVADAAIENGVEKVLALSTDKAVNPINLYGATKLAAEKLLIQSNAYAGGRST
                     RIACTRYGNVVGSRGSVVPLFLQQRPSGHLTITDERMTRFWISLEQGVRFVIRCIEQM
                     QGGEVFVPKIPSMSMMDLAKAIAPEAQVDIIGIRPGEKLHETLISEDEARTTVELPDM
                     FVVQPASAMWFGRAWEKQGKPLPDGFRYSSNNNSDWLDLSRINEMIAPIEKDFEKDQL
                     P"
     gene            7680..8600
                     /locus_tag="PKL60_02945"
     CDS             7680..8600
                     /locus_tag="PKL60_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW95124.1"
                     /translation="MKKLLVTGASGLLGLNLALKAADLGWDVTGWSGRRALARVPFHA
                     EQVDLANLEDIPMRIGALSPDLVIHCAALADIDKAEQHPALAEKLNALAPEILAQAAR
                     SAGARLIHISTDAVFDGQRGDYRESDEPRPINTYARTKLAGERAVLAAYPEALAARVN
                     FYGWSPSGQRSLAEFFYTHLRSGQTVNGFTDVFFSPLYVGHLAEILLKIAEKRLEGLY
                     HVFCPQATSKYAFGVSVARLFGLDESLINPAALQEQKTGTPRPLNLSMNTDKLQAALD
                     EPLPGEAEGLQAFYADWRAGLPDRLVAAIV"
     gene            8638..9705
                     /locus_tag="PKL60_02950"
     CDS             8638..9705
                     /locus_tag="PKL60_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNW95125.1"
                     /translation="MEITIGKHTLGEAHPTYFIADIAANHDGSLDRALELIRLCAQAG
                     ANAAKFQNFRGPKIVSDYGFTHMNAQVSHQAKWRKSVVEVYNDASIPFEWTPILKQAC
                     DEAGIEYFSSPYDFEAVDMLDPFVPAYKIGSGDITWLEACQRIAGKGKPVLLAAGASD
                     IGDVQRAVDAILEVNPSLVLMQCNTNYTAADGNFDNINLNVLKTFHAMYPQLVLGLSD
                     HTHGHAAVLGAIALGARVVEKHFTDDNLREGPDHAFAMNPRTWAEMVTAARQLERALG
                     SGNKHVADNERETVVIQRRCLRAARAIQAGESFTREMIDVLRPAPAGAIMPYDIQAVL
                     GTRALVAIEPGQELRWTMLGA"
     gene            9728..10837
                     /locus_tag="PKL60_02955"
     CDS             9728..10837
                     /locus_tag="PKL60_02955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNW95126.1"
                     /translation="MRVLYCTRSDSPHDRRFLTALGQSGHEVYALRLFGGTPLTPKGV
                     TEVPWQGLSGALRLSDLPGQLRQLRRLLTNLRPDLVHAGPIQDLAFLAARAGFHPLLT
                     MSWGFDLMKDAYQSPLRRWQTRYTLKRSDYLTLDCQATADRAAAFGFPRERICVFPWG
                     VDLDFFSPQNSVVPGKSWRAAQGWEEKTVLLCLRSWEPNYGVDVLARAFARAVKENPQ
                     LRLILLGDGSQGPKIRALLRGAENAVYFGGRVELSALPGWYGAADLYVSPSHVDGSSV
                     SLLEAMACGLPSIVSDIPANLEWIQDGLNGWVFPDGDPEALTRIILAAAQQDWRAYKL
                     QARQDAVLKADWNKGVRKLLACYEEAAAFGESRKL"
     gene            10834..11481
                     /locus_tag="PKL60_02960"
     CDS             10834..11481
                     /locus_tag="PKL60_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95127.1"
                     /translation="MKKNILLLVCALSLLAAALACNLPLNSRSEPEPTPTPDYGMIWY
                     QGVGMQILLPNTYVASSIQEDMPGILAAIRSFIGDGPSPLSSLVDNLEGNVSWWGYDA
                     GAPAVSPVQLLIVKNESLEKTPLSLISTALRLFLGKDAESLQTTSYTTDGRDVTRFSY
                     AHENTGWQAYIFKEQGQLWVALFISPPANLAAQQVYYEYSVNSMLIAPLQETPQP"
     gene            11495..13231
                     /locus_tag="PKL60_02965"
     CDS             11495..13231
                     /locus_tag="PKL60_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNW95128.1"
                     /translation="MTEPKILAIIQARMSSSRLPGKVLRDLGGKPVLERVISRVSRAR
                     HISGLVVATTSDSSDDPIEAFCVQKGTPVFRGSQYDVLDRFYQCARQYQADVIVRITA
                     DCPMIDPQEIDRVIAAFLEGDCDFAANRLPPPQKRTSPIGMDTEVCSFAALEQAWQHA
                     AENFEREHVLPYLYDTPGRFRVRLVDTAPDMGQLRFTVDTEEDLQVARAIYAGFGNSD
                     DFSLEDLLRLSAQNPQWQHSLAGVQHKSFLDVDERAGAKLQEPASPPAADAAEAPVAP
                     EAPSNAGAFAHASPRCPLCGARQARVLEKVSSFGFSVPYYLCKACGFVFQDAAASKAA
                     EPAFYAETYRKLYQASEEPTPKDLRQQSLRAAEQVRFLHSCPVKTLGRVLDIGASSGR
                     MLLALREAYQAEAVGVEPGNAYRALAEAEGLRMYPSLESLLASQPLPFDLVSLMHVLE
                     HFDDPLGSLRQIREKLLSPAGWLLLEVPNFYAHNSYELAHLSCFTPASLKEMLRKAGY
                     RVVSLRKHGYPRSKLFPLFLNVLAVPLENPPELAETRVERCVPVRRKLAMLRRRVLSR
                     LLPRRAWLPLEG"
     gene            13235..14167
                     /locus_tag="PKL60_02970"
     CDS             13235..14167
                     /locus_tag="PKL60_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95129.1"
                     /translation="MPLITLFSAPKPFTNPHIALIQRNALASWRALGPEVSAALVGDE
                     PGIAEAAAEFGCTHLPQVATNALGTPLISSIFALGRSLNDSPFLAYVNADILLFPDFL
                     DCTRQVGDVFERFLLVGRRWDLRVEETLRFDPEGQSALRQKLNAGGRLHARTGSDYFI
                     FPRACFKQVPDFTVGRAGWDNWMIYQARAQRWPVVDASADLQIIHQDHDYSHLPDGQR
                     HYRLPETGENVRLAGGWRTIFMLDDASHQLRGGKISAFPLSWKKFWREVQHYPLLKWH
                     NYALTQILFTLFHPKRAWGEFWKNRAFQKASKEA"
     gene            14171..15181
                     /locus_tag="PKL60_02975"
     CDS             14171..15181
                     /locus_tag="PKL60_02975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNW95130.1"
                     /translation="MPRIGVNPSRSQTVSFRPPRVTVAVLVYAPHQAGYFQHRMDVTR
                     LTLESILANTEEPYELLVFDNGSCPEMVSTLQELFAQGKIDRLVLSAQNIGKLNALYR
                     IARLASGSVIAYSDDDVYHLKGWLPAHLEVLDTFPNVGAVTGFYIRQRVVMSSDSTLA
                     WAENSAEAVERGLLMPRKWEEEYMDNSGRTEARYQSEVAGVEDVVVSYHGLKAWVSAH
                     HFEVLTPKKVLLEVLEEMLADGWSDQLMGRMVEMDDRMDAKGYLRLTTYTQTMRLLGN
                     ALDEEVAALAAQDGIRAQAAASPRPRSGLLTRLAANRRVRNGIQGLINWLYGLLHQNG
                     RE"
     gene            15178..16173
                     /locus_tag="PKL60_02980"
     CDS             15178..16173
                     /locus_tag="PKL60_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNW95131.1"
                     /translation="MMRFLIVGLGSIGRRHLRNLCALGQQDILLYRTHRATLPDEELD
                     GLPAFTDLAEALAEKPQGVIIANPTSAHLRAALPAAQAGANLLIEKPVSHTTEGLDEL
                     KEVLTLKHTRAMVGFHFRYHPVLGELRQILTEGSLGWTLSGCARWGEYLPGWHPWEDY
                     RVSYAARADLGGGVTNTLSHPFDYLRWMLGEVVSVRAETSKLSDLELDVEDNSEALLE
                     FANGARVSVHLDYYQQPASHTLELTCERGRILWDNADGSARLLAADGESNRQIIPPEG
                     FERNDMFLAEMADFIRLCRGEKFEYCSLEDGIRVQQIVEAVRQSHALEGRRVSLE"
     gene            16233..17060
                     /locus_tag="PKL60_02985"
     CDS             16233..17060
                     /locus_tag="PKL60_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW95132.1"
                     /translation="MEQNVLNKFDMSGRAVLVTGAGGLLGRQFSLALAQAGASVMLAD
                     LAENLAQGQAQHIRSLGLSAEALRVDVIDPDSTQAMVAKTLERFGRLDVLINSAAMDP
                     KFDPQHQSQQAANAFESYSLENWRKALDVNLTGMFLASQAAVAPMLAQGAGVIVNICS
                     TYGLNGPDQRIYERPDGSRSFKPVYYSVTKAGVLGFTRYLAAYYAGKNIRVNALTPGG
                     VYNGHDEHFTQSYSAKTILGRMANLDEMSAAMLFLASDASSYMTGSNLVVDGGWTAW"
     gene            17054..18304
                     /locus_tag="PKL60_02990"
     CDS             17054..18304
                     /locus_tag="PKL60_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HNW95133.1"
                     /translation="MVKQPEVLALIPARGGSKGIPRKNIREFAGAPLIAWSIAAALRA
                     ETVTRVIVSTDDEEIAAVAREWGAETPFLRPAELAQDDTADYPVFRQALDWLAAHEDY
                     RPEVVVQLRPTSPVRPLHLVDDAVRLLLAHPDADCVRGVVPSGQNPYKMWKIDAESGR
                     MLPLLQVEGLAEPYNAPRQSLPKTYWQTGHIDAIQVSTILEKGSLTGDEIWPLMIDPR
                     YTVDIDTPADWERYERLLQDPHIQAVDPLVSRRAFPARVRLLLMDFDGVLSDDLVLTD
                     QDGRESLRCSRSDGFGLSLLREKTDIQAAVLSREENPVVSARCRKLKLEVFQGVRQKD
                     QALKALIQARGWNPAEILYLGNDLPDLPVLPLVGYFAAPADAHPDVLRRADLILKHRG
                     GRGAVREICERLLLETHKTNLETL"
     gene            18301..19176
                     /locus_tag="PKL60_02995"
     CDS             18301..19176
                     /locus_tag="PKL60_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNW95134.1"
                     /translation="MNREVTIGNRKVGDGHPAFIIAEIGINHNGSLEFAKQMIFAAHQ
                     AGVDAVKFQKRTPELCVPEHQRSQMRETPWGYIPYLEYRYKVEFGQEDYEEIDRYCKE
                     LGIAWLASSWDIPSVEFMEQFNPPAHKIPSALLTDHALLRAYRKTGRPLILSTGMSTL
                     EEIKASVALVGEENLILCHTTSSYPCPPDELNLRMIQTLREMFRCPIGYSGHEVGLVP
                     SAVAVALGACLVERHITLDRAMWGSDQSASVEPQGIERLVKYIRVTEQALGDGVKQVY
                     ESEKSSLSKLRRFSQ"
     gene            19179..20795
                     /locus_tag="PKL60_03000"
     CDS             19179..20795
                     /locus_tag="PKL60_03000"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95135.1"
                     /translation="MAANTLVERARRRLAISRAHQISDQRTRSIAELVAARAPQPTKA
                     PVVFFNASTRLSGVSLNAAFGMLAGWSLRLQGVPVVNFVCKRGMTQCVLGTDRREPPK
                     APPCAVCMRQSRVLYHGGENVVFEFQPDAALDAALESLDLAGLSAFTWQGMPLGELVL
                     PSARWILRRHHLHEDPTTLRILREYIRSAWSVGAQFHKLLDEVRPAAVVVFNGMQYPE
                     ASARWVARQKGLPVYSHEVGLRPMSAFFTPGDATAYPLALPEDFELDADQNARLDAYL
                     QNRFQGNFIMAGVRFWPEMSALDPEFLTLAKGFRQIVPVFTNVIFDTSQPHANVLYED
                     MFTWLDEVLETARAHPDTLFVIRAHPDEARKGKASEESVAEWAERRQVEQLPNVRFIR
                     PETYISSYDLIRMAKFVMIYNSTIGLEASILGALVLAAGRSRYSQADTVRFPASRQEY
                     QRELEALLLAPEARAPERYRQNARRFLYYQLYRSSLPFDAFLEEDQVWAGYVRLKDFE
                     PRALLPENSPTLRAISEGLFAGGDFLLKEE"
     gene            20796..21476
                     /locus_tag="PKL60_03005"
     CDS             20796..21476
                     /locus_tag="PKL60_03005"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNW95136.1"
                     /translation="MSLEKIVALVPMRHASVRVPGKNYRPIAGKPLYHHILETLLACP
                     EISQVVVDTDSPLILDGLAKDFPTVAALLRPEHLRADTIPTNEILLHDTAQVPADLYL
                     QTHSTNPLLRTQTVSAAIRALREVYPAYDSLFGVTRLQTRLWDELGRAINHNPAVLLR
                     TQDLPPVYEENSCIYLFTRETLAQRRNRLGERPLMFEIAAEEAWDIDEEIDFTIAEGL
                     LRARKEAK"
     gene            21473..22453
                     /locus_tag="PKL60_03010"
     CDS             21473..22453
                     /locus_tag="PKL60_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNW95137.1"
                     /translation="MKDVLISAPYMLPFLPRFTPVLEQLGMRVLLPEVHERLSEEELL
                     AYAGRFDGTICGDDRYTRRVIQACLPRLKVISKWGTGIDSIDQEAAAEFGVSVRNTPN
                     AFTVPVAESVLGYMLNFARHFSAMDSAMKAGEWQKIPGYTLSEKTLGVVGVGNVGKAV
                     LRRARAFGMKLLGNDIIEIAPDFLLENSVEAVPLRELLERADFISLNCSLTPSSYHLI
                     NAQTLSLVKPGALLINTSRGPVVEEPALVRALQAGRLAGAALDVFEVEPLPADSPLLT
                     MKNVLLAPHNSNSSPFYWERVHWNSIRNLALGLDLPVGDIASIKALEKQA"
     gene            22467..23234
                     /locus_tag="PKL60_03015"
     CDS             22467..23234
                     /locus_tag="PKL60_03015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW95138.1"
                     /translation="MTEQSQRVVLITGAAGGVGQAAVNHFFARGWRVIGVDRKPVYAR
                     FPADGLFIQADLALPQEVERVYREAAEFTPKLDALVNNAAFQVTKPISQTSVEEWDQV
                     IAVNLRSAFFGARYGFALLAAEGGGAIVNVASVHAVATSPNIAAYAASKGGLVALTRA
                     MAIEFAPANIRVNAILPGAVDTPMLAAGFNRGHLTQGSIEQQKAELARKTVNGRIGQP
                     HEIASVIYFLADNTQSSYMTGQSVVVDGGASARLSTE"
     gene            23239..24045
                     /locus_tag="PKL60_03020"
     CDS             23239..24045
                     /locus_tag="PKL60_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNW95139.1"
                     /translation="MPEKTDLNLPPPLMETARRLYDGARRLPELPAAYLHPWRRESIR
                     RLSLLRDAHKGERCFLIGNGPSLKRTDLSLLRNEFSFGFNRIFLAAEELNFTPSCLVS
                     INDLVIEQSAPEFRALEMPKFFAWRSHRWVGMDAHTHYLYTSYTTPKFARDVRGRVWE
                     GATVTNVALQLAYFMGFSTVILVGVDHSFATQGRPNTTVQSEGDDPNHFSSAYFGKGF
                     RWQLPDLETSELAYRMARKAYEADGRQVLDATIAGKLEVFPKVAYESLFG"
BASE COUNT         4746 a         7453 c         7325 g         4716 t
ORIGIN      
        1 tctgcctgcg aatgttgggt tacgctcctg cgtcgctgca cccaacctac aaacttgagc
       61 gcggaggtct gcctgcgaat gttgggttac gctcctgcgt cgctgcaccc aacctacaaa
      121 ctgcacccgg attaaacggc ttgccatcct gagcgcgagc gaaggatctg gttcttccac
      181 aatagaggtt cttcatttcg gcctgtgacc tttagggtat cagcatgaca ggcatcgggc
      241 gggcgcttcg cccctttccc tgagcaaagc agttctccgt cggagtgtag agtacaaacc
      301 gacttgcatc acgatcacaa cgcatatccc ccctctcctc gacttgacgc cttggaatta
      361 gcgtctataa ttcaacaact gaaatatatg gaagcagaac tgtccgccga agagaacagc
      421 cgcacgctgg atctccttac ccaaatcgaa gccaaccccg acatttcgca agccagtatg
      481 gcggatgcgc tcggcgttgc cgtgggcacc atcaactggt acctcaaacg cctgatatcc
      541 aaaggctatg tcaaggtcaa acgcgcccag cgtaaaaagc tgcgctatat catcacgcct
      601 gaaggcatcg ccctgcgcgc cagcctgacc gttgattaca tccgcacctc gttcgacctg
      661 taccgccgcg tgcgggagcg ctccgccaaa tgcatcgagg agttgcgcca gcacggctgt
      721 gacagcgtgc gcatccttgg cgacggtgaa gtggcggagg tgatccggct gacctgcatt
      781 gagcacggcg ttgaactgac cgaggcggaa ggcgcgcctg aaattcacat acaaggcatt
      841 aaacttgcac ttgtttttcc agacgcggag gcagcttgcc ctcccgcgca gctaatcgtc
      901 taccgaggga actatggaac aggaaattaa cttttccgga aaacggatct gcgtcaccgg
      961 cggcgcgggc tttttgggca cgcacttaat tgccaacttg cgcacaaaag gcgctcagga
     1021 tattttcatc cccaaatatg aagactacga cctggtgaaa ccggatgata tcgcgcgcat
     1081 gctggaagac tccgcgccgg atatcctcat ccatctggcg gcgcacgtgg gcggcatcgg
     1141 cgccaacatg gccaagcccg ccgagttctt ctacgacaac ctgatgatgg gcgtgcagct
     1201 gatgcaccaa gcctggcagc gcggcgtgga aaagttcgtt gccatcggta ccatttgcgc
     1261 ttaccctaag tttacgccca tcccgttcaa ggaagagcat ttgtgggacg gctaccccga
     1321 ggagaccaac gcgccctacg gactggccaa aaagatgctg ctcgtccagg cgcaggctta
     1381 ccgccagcag tacggctata acgcgatttt cctgctgccg gtcaaccttt acggcccggg
     1441 cgacaacttc aaccccgcca gttcgcacgt gattcccgcg ctcattcgca agtgcctgga
     1501 ggcaaaggac caaggcgcca gcgaaatcgt ggcctggggc gacggctctc ccacgcgcga
     1561 gtttatctac gtggaagacg ccgcggaagg catcgccctg gcaacgcagc gctataacgg
     1621 cgcggaaccg gtcaatatcg gctccagctt cgaaatcagc atcagagacc tgacggaact
     1681 catcgcgcgt ctgaccggct tcgacggcgc tatccgctgg gataccagca aacccaacgg
     1741 ccagccgcgc cgcaagctgg ataccaaccg cgcgcgtgaa tactttggtt tcagcgcgaa
     1801 aacggacttc gaagagggtt tgcgccgcac catcgcctgg taccaggacc ataggagctg
     1861 aagcgcgtga gcgaaacccc ggtttccgcc aagatggttg tgcttcgccc ttccaagggt
     1921 tggctgggca tcgacctgaa agagctctgg ttttaccgtg agctgattta ctttttaacc
     1981 tggcgcgata tcaaagtgcg ttataagcag gctgccctgg ggattgcctg ggcggtgctg
     2041 cagccggtgc tcaccaccgc gattacctcc gtggtcttcg gcgtgctgct gaaagtggaa
     2101 tccgacgggc tgccctatcc ggttttcgtg ctcgcggcgc tcctgccctg gcaccttttt
     2161 cagctttccc tgcagaaatc cagcatcagc ctggtgggca acgccaacct gattaccaaa
     2221 gtgtactttc cgcgcgtgat aatcccgctt tcttcggtgc tggcggtgat ggtggatttt
     2281 gccatctcgc tggtgctgct ttttatcgcc atggcgattt tccacatgcc cattacgctc
     2341 aacgcgctct ggctgatacc cctcacgctt ttggcggtgt tggcagcgct gtcggtgggc
     2401 ttgtggctct ccgcgctgaa cgtacagtac cgcgacatcc agcagattgt gcctttcctg
     2461 gtgcaaatct ggatgtacgc ctcgccgatt gtctacccta tcagcaccat tccggaaggc
     2521 acgctgcgca gtctgtacag cctgaacccc atggtagggg tggtccaggg cttcaggtgg
     2581 gctttgtttg ggggcacgcc gccggacttc acgctcatta tttccagcgc ggtggttgtg
     2641 ctgctgctga tttccggctt gttcttcttc cggcgcatgg aaaaaacttt tgcggatgtg
     2701 gtgtaatgaa cgcttctgaa tactccatcc aggtaaatgg catttcaaag cgctataaaa
     2761 tcggcaccgc ccaacagcgc gctgataccc tgcgcgacct catcaccaac caggtacaac
     2821 gaattgggca aagcttctcg cgcggggggc gcgccaataa aagcgccgat accatctggg
     2881 cgctcaagga tgtcacattc gacgtccggc aggggcaggc gctgggcatc atcgggcgaa
     2941 acggcgccgg caaaagcacc ctgcttaaaa tcctctcgcg cgtaaccgac ccaacggagg
     3001 gctacggcga actgcgcggg cgcgtaggtt cgctgctgga agttggtacc ggctttcatc
     3061 cggaactgac cggacgggag aacatcttcc tcaacggcgc gattctgggt atgacccgca
     3121 aagaaattct gacccgcttt gacgaaatcg tcgatttctc ggaggtcggg cagttcatcg
     3181 acactccggt caaacgttat tccagcggta tgtacttgcg cctggctttt gccgtggcgg
     3241 cgcatctgga gccggaaatt ctggttgtgg atgaagtgct ggcggtcggc gacgcggatt
     3301 ttcagcgcaa atgcctggga aagatgggtg atgtggctca gcaggggcgt accgtgctgt
     3361 tcgtgagcca caacatgtct tccattctgc gcctgaccga ggaaaccctg gtgttggaga
     3421 agggcaaagt gctgctgcgc gcgccttcca cggaagcggt ggatttttac cttaaccgcg
     3481 gcctcagcaa gctgggcgag cgcttttggg aggaggacga agtgcctgct tctgccgcgc
     3541 cttttcgccc cctggcaatc cgcgtgcgcg ccaaggacgg cgcggtctcg gataccgtgc
     3601 gcagcgtgga gcccttcacg attgaaattg agtacacgct gagcgaagac ctgaccggtt
     3661 tgcgcgtggg cttttacctg atgtccaccc gtggtgagcc catcttcacc agtttcgata
     3721 ccgattccag tgagctgttc caggcgtatc ccacccgtgc caagggcgcc tacaccagcc
     3781 gctgtgtaat tcccgccaat accctgaatg aaggccgctt tgttttgggc atcaatgcca
     3841 gctcttacca tatccggcgc tattttcagg acgagcaagc gctgaccttc tcggtggatg
     3901 ccagcggcgc gccgggcacg cactggcctg agcccagacc gggcggcgtg cggccggtgt
     3961 tggactggca gattgcgcgg gaggagtgag catgggcgtc cgtcagcaca tgaaaaactt
     4021 cctggacgac ctgccctttg gcgcggatct gtactggcag ctgagaggga aacgcaaacc
     4081 ctggtcggcg cattatgagc tggaggacct aaaagccgtc ctgccgcagg ctgtcgcgga
     4141 cctgtccgca gcgcgggcgc agcaaggaga cctccccaca gaaccttcgt cattgcgaac
     4201 gcagcgaagc aatctccccg ctgatctatc ctcgtcatcc caagatcagc taagcaatac
     4261 tcaacaaggg acacaatgtc tccccattga acaagaggga gattgccgca gtcgcttacg
     4321 ctccttcgca atgacggatc accaacccaa aaaaatctgc ctgttcgcct ccctgcacta
     4381 ctggatcgag gaaagcgcgc tcattggcct ggctctggcc gggcaagggc acagcgtgaa
     4441 cctggcttac ctgccctacg cggaatggga taaggaaatc acacccttcg acctgcgccg
     4501 gcaggacctg tacactcgcg aggtcttcgc ggaagccaaa aacttcctgc gcctctcgcc
     4561 cctcgcggat gaaaaagttt cccctgaacg cctgcccgcg gaggcgctgc gggcggttga
     4621 gcaggtctcg gactacgaca cgcagtacac cctgcaggtg gaagaaaccg accgcggcag
     4681 cgcgctctac cagctgcgct acgcccgcaa cttgcaggcg gccggcgcgc tgtacgcctg
     4741 gctgagggct gaaaaacccg atgtgctgat tgtccccaac ggcaccatcc tcgagatggg
     4801 cgtggcatac cgcgtcgcgc agctgctggg tctgcgcgtg gtcacctttg agtttgctga
     4861 ccagcgtgag cgcatctggc tggcgcagga tgatgaaatc atgtcgcacg ataccaacgc
     4921 cctgtggcaa gcgctcgggg gccaaagcct gcccgaagcc gcccgccgcg cgatgctgaa
     4981 tctctatgcc gcccgccgca acgccaggct gtggggcaat tttgcccgcc aatggcagca
     5041 aaccccactg caaggcgccc gggaagtgcg cgacaggctg ggtttggacc gccgccctgt
     5101 ggctctgctg gcgaccaacg tcctgggaga cagccttacg ctcgggcgcc aacgcatctc
     5161 ggcgacgatg gcggactgga ttgtgggcac gctggcctac tacacgcgcc accccgaagc
     5221 gcagttgctg gtgcgcatcc acccgggcga actgctgacc cacggtacct ctatggtgga
     5281 agtcatccat gccaaattcc cgcagctgcc ggaaaatatc cacatcatcc ttcccggaga
     5341 caaaaccaac acctacgacc tggtagaaat cgccgatttc gggctggtct ataccaccac
     5401 cgttgggatg gaaatggcga tggcggggct gcctgtgatt gtggcaggca agacgcacta
     5461 tgccggcaaa ggctttaccc tcgaccccga atcctgggag gagttcgaag atttgctctc
     5521 gcgcctgctg gccgacccgg ctgccttccg cctgaatccg cggcaggtgg agcaggcctg
     5581 gctgtacgcc tacctgttct ttttcgaatt ctcgctgcct ttcccctggc atttgctctg
     5641 gctggcagac gatttttccg ccagaccgct gcgcttcgtg ctttcgcctg agggtcaggc
     5701 gcgcttcgcg ccgaccttcg ggtacctggc gggcgagccg ctggattggg ctgcccgcgg
     5761 attggccagg ctgaacgaac tgcccgcgga aactgaggag agcgcatgac ccaacacgac
     5821 gtcaagcagg aaatcggacg cttctacaac gaggtcggat ggcagctgga gcaggacggc
     5881 aatttccaga atgcccaata cgaggacttg cgcccggttt ccgctgagta catcgcccgg
     5941 gcgcacgccc gcgtggcgag gcacctgccc aaaagcggtc gctacctgct ggacgccggc
     6001 tccggacccg tccagtacga cgcgtacctg cagtactcgg ctggttacga gcgccgggtg
     6061 tgcctggacc tgtccttcgt ggcgctcaag caggcgcgtg ccaggctggg cgcgcgcggc
     6121 tggtacgtgg tcgcggatat tgccaacctg cccttcaaac ccgaagcctt tgacggcatt
     6181 gtttcgctgc acaccatcca ccacctgccg accgatgaga agcccggctg ttacatgggc
     6241 ttgtacgagc gcctgacccc cggcggcagc atggtcaccg tggacggctg gtcagagcac
     6301 aagctcagga agacctggac ctggctgatt cggctggcgg aaaaactgcg ccggcagaaa
     6361 tcgcccgggc aggaggaagg cgcgcgagcg gcagcagaaa gcaaacccgc cggcacctac
     6421 gtggtcaaaa acaacgctgc ctggttcaaa gcggctgttg gcagccagct gcccgcgcgg
     6481 atattcacat ggcgcagcgt cagcgtgcac tttctgcgca ccgtcatcca gccggaatgg
     6541 ggcggcaaat tctggttaaa agtgctttat cggctggagg aatgctttcc ccgctatttc
     6601 ggcgagaacg ggcagtaccc gttaatcgtg attagtaaac cgaataaaga cgcacccgca
     6661 taacaaggag tctgtatgga atggaacaat aaagtggtac tggtcactgg cggaaccggt
     6721 tcctttggca agaaactcat caaaatattg gtcgaggaat accatcccgt caaaattatc
     6781 gtcttcagcc gggatgagct caagcagcac gaaatgcgcg tcagcggtta taaccatccc
     6841 tccatccgct atttcatcgg ggacgtgcgc gaccgcgaac gcctttcgcg cgcgttttac
     6901 ggcgtgaata tcgtcgtgca cgcggccgcc ctcaagcagg tgccggcttg cgaatacaac
     6961 ccgatggaag cgattaaaac caacatcctc ggctccagca acgtggcgga cgccgcgatt
     7021 gagaacggcg tagaaaaagt gctcgcgctg agcacggata aggctgtgaa cccgattaat
     7081 ctgtacggcg ccaccaaact ggcagccgag aagctgctca ttcaaagcaa tgcctacgcc
     7141 ggcgggcgca gcacgcgcat cgcctgcacc cgctacggca acgtggtcgg gtcgcgcggc
     7201 agcgtggtgc cgcttttcct ccagcagcgc ccgagcgggc acctgaccat caccgacgag
     7261 cgcatgaccc gcttctggat ttcgttggaa cagggtgtac gctttgtcat tcgctgcatc
     7321 gaacagatgc agggcggcga agtgttcgtt ccgaaaatcc cgagcatgag catgatggac
     7381 ctcgccaaag ccatcgcccc ggaagcgcag gtggatatca tcggcatccg ccccggcgaa
     7441 aagctgcacg aaaccctgat ttccgaggat gaagcccgca ccaccgtgga actgccggat
     7501 atgttcgtcg ttcaaccggc ttccgccatg tggtttggac gcgcctggga gaagcagggc
     7561 aagccgcttc cggatggttt tcgctattcc agcaataaca acagcgactg gctggacctc
     7621 agccgcatca acgaaatgat tgctccgatt gaaaaggact tcgagaagga ccaattgcca
     7681 tgaagaaact tctggtaacc ggcgcgagcg gactgctggg gctgaacctg gcactcaagg
     7741 ctgctgacct gggctgggac gtgaccggct ggagcggccg ccgcgcgttg gcgcgcgtgc
     7801 cattccacgc tgaacaagtg gacctggcaa acctggagga tatccctatg cggattggcg
     7861 cgctttcccc tgatctggtc attcactgcg ccgcgctggc ggatatcgac aaggccgaac
     7921 agcaccccgc gctggcggaa aagctgaacg cccttgctcc agagattctc gcgcaagcgg
     7981 cgcgcagcgc aggcgcccgg ctcatccaca tttccaccga cgcggtcttt gacgggcagc
     8041 gcggcgatta ccgcgaaagc gacgagccac gcccgattaa cacttacgcc cgcaccaagc
     8101 tggcaggcga gcgcgccgtg ctcgcggctt accctgaggc gctggcggcg cgcgtgaact
     8161 tctacggctg gtcccccagt gggcagcgca gcctggcaga attcttctac acccatctgc
     8221 gctccggaca gaccgtgaac ggctttaccg atgtgttttt ctcgccgctg tacgtcgggc
     8281 atctggctga aatcctgctg aagatagcgg agaagcgcct ggagggcctg taccacgtgt
     8341 tttgcccgca ggcgaccagc aaatacgcct tcggggtcag cgtggcgcgc ctgttcgggc
     8401 tggatgagag cctgattaac ccggcagcgc tgcaggaaca gaagaccggc accccgcgcc
     8461 cgttgaattt gagcatgaat accgacaaac tgcaagccgc gctggatgag cctttgcccg
     8521 gcgaggcgga aggcttgcaa gccttttacg cggattggcg ggccggctta cccgaccgcc
     8581 tggtcgcggc cattgtctga gtatcacgtt atcagtgagc tgcataaagg agatactatg
     8641 gaaattacaa tcggcaaaca tacactggga gaggcgcacc caacctattt catcgcggat
     8701 attgccgcca accacgacgg cagcctggac agagctctgg agctgattcg gctgtgcgcc
     8761 caagccggcg cgaatgccgc caaattccag aacttccgcg gcccgaaaat cgtctcggat
     8821 tacggtttca cgcatatgaa cgcccaggtt tcgcaccagg cgaaatggcg caagagcgtg
     8881 gtggaagtct ataacgacgc ttccatccct ttcgaatgga cgcccatcct caagcaggcc
     8941 tgcgacgaag ccggcatcga atacttctcc tccccctacg attttgaggc tgttgatatg
     9001 ctcgaccctt ttgtgccggc ctacaaaatc ggctccgggg atatcacctg gctggaagct
     9061 tgccagcgca tcgccgggaa aggcaaaccg gtgctgttgg cagccggcgc ctcggatatc
     9121 ggcgacgtgc agcgcgcggt ggacgccata ttggaggtca accccagctt agtgttgatg
     9181 cagtgcaaca ccaactatac cgccgcggac ggcaactttg ataatatcaa cctgaacgta
     9241 ctcaagactt tccatgccat gtacccgcag ctggtcctgg gtctttcgga ccatacccac
     9301 gggcatgcgg cggtcttggg cgcgattgcc ttgggggcgc gcgtggtgga gaagcacttc
     9361 acggacgata acctgcggga gggccccgac catgccttcg caatgaaccc gcgcacctgg
     9421 gccgagatgg tgaccgccgc ccgccagttg gaacgcgccc tgggcagcgg caataagcat
     9481 gtggcggata acgagcgcga aacggttgtt atccagcggc gctgcctgcg ggcggcgcgt
     9541 gccatccagg caggcgaaag tttcacacgc gagatgattg acgtcttgcg ccccgccccg
     9601 gctggcgcga ttatgcctta cgacatccag gctgtgcttg gcacccgcgc gctggtggcg
     9661 attgaacccg gccaggagct gcgttggacc atgctcggcg cttgagtgtg ttgaggagag
     9721 cgtagctttg cgcgttttgt actgcacccg ctccgacagc ccgcacgacc gtcgcttcct
     9781 gaccgccctc ggtcagagcg gtcacgaagt gtatgccttg cgcctgttcg gcggcacacc
     9841 cctcaccccc aaaggcgtga ccgaggtacc ctggcagggc ctcagcgggg ctctcaggct
     9901 cagcgatctg cccggccagc tgcgtcaact tcggcggctg ctgacgaacc tgaggccgga
     9961 cctggtacac gccggaccca tccaggacct ggcgtttctg gctgcccgcg cgggtttcca
    10021 cccgctgctg accatgagtt ggggctttga cctgatgaaa gacgcctatc aatcgccgct
    10081 gcgccgttgg caaacacgct acaccctgaa gcgctccgat taccttacgc tggactgcca
    10141 ggctaccgcc gaccgcgctg ctgcttttgg cttcccaagg gagcgcattt gcgtcttccc
    10201 gtggggcgtg gacctggact ttttctcccc tcaaaacagc gtggtgcccg gcaaaagctg
    10261 gcgcgctgcg cagggctggg aggagaaaac cgtgctctta tgcctgcgct cctgggagcc
    10321 caattacggc gtggacgtat tggcgcgcgc ttttgcccgg gcggtaaaag agaacccgca
    10381 gctgcgcctg attttgctgg gcgacggctc tcagggtcca aaaatccgcg cacttctgcg
    10441 gggcgcagaa aatgcagtgt atttcggcgg gcgcgtggag ctctccgcgc tgcccggctg
    10501 gtacggcgcc gcggacctgt acgtcagccc ctcccacgtg gacggctcgt ccgtttcgct
    10561 gttggaagcc atggcctgcg gcttgccctc gattgtttcg gacattcccg ccaacctgga
    10621 atggattcag gatgggctga acggctgggt cttcccggac ggggaccccg aagcgcttac
    10681 ccgcataatc ctggcagccg cgcagcagga ttggcgcgcg tacaaactgc aggcacggca
    10741 ggacgccgtg ttgaaggcgg actggaacaa aggcgtgcgc aaactgctcg cctgttacga
    10801 agaggccgcc gcatttggag aatcgagaaa gctatgaaaa agaacatcct cctcctcgtt
    10861 tgcgccctta gtttgctggc ggctgccctc gcatgcaacc tgccgctcaa ctcgcgttcc
    10921 gaacccgaac caaccccgac gccggactac ggcatgattt ggtaccaggg cgtaggcatg
    10981 caaatcctgc tgcccaatac ttacgtcgcc agcagcattc aggaagacat gcccggcatc
    11041 ctggctgcca tccgctcctt tataggcgat ggtccgtccc cgctttcctc cctggtggac
    11101 aacctggagg ggaacgtctc gtggtggggg tacgacgcag gcgcgcccgc ggtttcgccg
    11161 gtgcagctcc tgattgtgaa gaacgaatcc ctggaaaaga ccccgctcag cctgatcagc
    11221 acggcgctgc gcctcttttt agggaaggac gcggaatccc tgcagaccac ctcgtacacc
    11281 acagacgggc gagacgtcac gcgcttcagt tacgcgcatg agaataccgg ctggcaggct
    11341 tacatcttta aggaacaggg gcagctgtgg gtggcgcttt tcatcagccc gcccgccaac
    11401 ctggctgccc agcaggtcta ttatgaatac agcgtcaact ccatgctgat tgccccgctg
    11461 caggagacgc cccagccgta aaggcggtga gcatatgacc gaaccaaaaa tccttgcgat
    11521 aatccaggcg cgcatgagtt ccagccgcct gcccggtaag gtgctgcgcg acctgggtgg
    11581 gaaaccggtg ctggagcggg ttatcagccg cgtcagccgc gcgcgccata tcagcgggct
    11641 ggtggtggcg accacctcgg atagttccga cgacccgatc gaggctttct gcgtccaaaa
    11701 gggcacgccc gtctttcgcg gcagccagta cgacgttttg gaccgcttct accaatgcgc
    11761 gcgtcagtac caggcggatg ttatcgtgcg catcaccgct gactgcccga tgatcgaccc
    11821 gcaggagatt gaccgcgtta tcgcggcgtt tctggaaggg gactgcgact ttgccgccaa
    11881 ccgcctgcct cctcctcaaa aacgcaccag cccgattggg atggatactg aggtgtgcag
    11941 ttttgccgcg ctggaacaag cctggcagca cgccgcggaa aattttgaac gcgagcacgt
    12001 tctaccctac ctgtacgaca cccccgggcg ctttcgggtg cgcctggtgg ataccgcgcc
    12061 ggacatgggg cagctgcgct ttaccgtgga taccgaggaa gacctgcagg ttgcccgtgc
    12121 gatttacgct ggttttggga attcggatga cttcagcctg gaagatttac tgcgtttgag
    12181 cgcgcaaaac ccgcagtggc agcacagcct ggcgggcgtg cagcacaaat cttttttgga
    12241 tgtggacgaa cgcgctggcg ccaagcttca ggaaccagcc tcccctcccg ccgcggacgc
    12301 ggcagaagcg cctgttgctc ctgaggcgcc ctccaacgcg ggcgcctttg cccatgcttc
    12361 accgcgctgc cctttgtgcg gggccaggca ggcgcgcgtg ctcgaaaagg tcagctcctt
    12421 tggcttcagc gtgccctatt acctctgtaa agcctgcggc ttcgtctttc aggacgctgc
    12481 cgccagcaaa gccgctgagc ctgctttcta cgcggaaact taccgcaagc tataccaggc
    12541 aagcgaggaa ccgaccccca aagacctgcg tcagcagagc ctgcgcgcgg cggaacaggt
    12601 gcgcttcttg cattcgtgtc cggtgaaaac cctcgggcgc gtgctggata tcggcgcttc
    12661 gagcgggcgc atgctgctgg cgctgcgcga ggcctatcag gcggaagcag ttggcgtgga
    12721 gcccgggaat gcctaccgcg cactggcgga agcagagggt ttgcgcatgt acccctcgct
    12781 ggagagtttg cttgccagcc agccgctgcc ctttgacctg gtcagcctga tgcacgtgct
    12841 ggaacatttt gacgacccgc tcggcagctt gcgccaaatc cgtgagaagc tgctttcgcc
    12901 cgcgggttgg ctgctgctgg aggtgccgaa cttttacgcg cacaacagct acgaattagc
    12961 gcatctcagc tgctttaccc ccgccagcct gaaggaaatg ctgcgcaaag ccggctatcg
    13021 ggtggtgagc ctgcgtaagc acggctaccc gcgctcgaag ctcttcccgc tctttctgaa
    13081 tgtgctggcc gtaccgcttg agaacccgcc cgagctggct gaaacccggg tggaacgctg
    13141 tgtgcccgtc cgccggaaac ttgccatgct gcggcggcgc gtactctcgc ggctgctgcc
    13201 gcgccgggcc tggctgcccc tggaaggatg agcgatgccg ctaatcacgc ttttcagcgc
    13261 gcccaaaccc tttaccaacc cgcacatcgc gcttattcag cgcaacgcgc tggcatcctg
    13321 gcgggcgctt ggcccggagg tgtctgcagc cctggtcggg gatgagcccg gcatcgcgga
    13381 ggccgccgcc gaattcggct gcacgcattt accgcaggtg gctaccaacg ccctgggcac
    13441 gccgttgatc agttccatct tcgcgctggg cagaagcctg aacgacagcc cgttcctggc
    13501 gtacgtgaac gcagatattc tgctcttccc ggacttcctc gactgcaccc ggcaagtggg
    13561 ggacgttttc gagcgcttcc tcttggtcgg gcgccgctgg gatttgcgcg tcgaagagac
    13621 cttgcgtttt gacccggagg ggcagtccgc gctcagacag aaattgaacg ccggggggcg
    13681 cctgcacgcc cgcacgggca gcgattactt catcttcccg cgcgcgtgtt tcaagcaggt
    13741 tcccgacttc accgtcgggc gcgccggctg ggacaactgg atgatttacc aggcccgcgc
    13801 gcaacgctgg ccggtggtgg acgccagcgc cgacctgcag attatccatc aggaccacga
    13861 ttacagccac ctgccggacg gtcagcggca ctaccgcctg ccggaaaccg gtgaaaatgt
    13921 gcgccttgct ggcggctggc gcacgatttt tatgctggac gatgcctcgc atcagctgcg
    13981 cggcggcaaa atcagcgctt tcccactcag ctggaaaaaa ttctggcggg aggtgcagca
    14041 ttatccgctg ctcaagtggc acaattatgc ccttacgcaa atcctcttca ccctgttcca
    14101 tcccaaacgc gcctggggcg agttttggaa aaaccgcgcg tttcagaaag ccagcaagga
    14161 agcttaagct atgcccagaa tcggcgttaa cccctcccgc agccagacag tcagcttcag
    14221 gccgccgcgc gtgaccgtgg cggtgcttgt gtacgcgccg catcaggccg gttacttcca
    14281 gcatcgcatg gacgtcaccc gcctgacgct cgagagtatc cttgccaata ccgaggagcc
    14341 ttacgaactg ctggtctttg ataacggcag ctgcccggaa atggtttcta ccctgcagga
    14401 gctgttcgcg caagggaaaa tcgaccgcct ggtgctttcc gcgcagaata tcggcaagct
    14461 gaacgcgctc taccgcatcg cccggctggc atccggcagc gtgattgcct acagcgacga
    14521 cgacgtctac catctgaagg gttggctgcc ggcgcacctt gaggttttgg atacctttcc
    14581 gaacgtcggc gcggtgaccg gcttctacat ccgccaacgc gtggtgatga gctccgacag
    14641 cacgcttgcc tgggcggaaa actccgccga ggcggtggaa cgcggcttgt tgatgccgcg
    14701 caagtgggaa gaagagtaca tggataactc cgggcgcaca gaagcgcgct accaaagcga
    14761 agttgccggc gtggaggacg tggttgtgtc ctatcacggg ctcaaagcct gggtcagcgc
    14821 gcaccacttt gaggtgctca cgcccaaaaa ggtgctgctg gaagtcctgg aagaaatgct
    14881 cgcggacggc tggagcgacc aactgatggg cagaatggtt gaaatggacg accgcatgga
    14941 cgccaaaggc tacttgcgcc tgaccaccta cacgcagacc atgcgcctgc tgggaaacgc
    15001 cctggatgaa gaggtcgctg ccctggcggc acaggacggc atccgtgccc aggctgctgc
    15061 cagccccagg ccgcgctccg gtttgctgac gcgtttggcg gccaatcggc gcgtgcgcaa
    15121 cggtattcaa ggcttaatta actggcttta cgggcttctg caccagaacg ggagggaatg
    15181 atgcgttttc tcatcgtagg tttaggttcc atcggcaggc ggcacctgcg caacctgtgc
    15241 gcgctcgggc agcaggatat cctgctttac cgcacgcacc gcgccacact gccggatgaa
    15301 gagctggacg gcttgccggc tttcacggat ctggctgaag cgctggcaga aaaaccgcaa
    15361 ggcgtcatca tcgccaaccc gacctccgcg cacctaaggg cggcgctgcc tgccgctcag
    15421 gctggcgcga acctgctgat tgaaaagccc gtctcgcaca ctacagaagg cttggacgaa
    15481 ttgaaggagg ttctgacgct caagcacacc cgcgccatgg tcggtttcca tttccgctac
    15541 cacccggttt tgggagagct cagacaaatc ctgaccgaag gcagcctggg atggacgctt
    15601 tcgggctgcg cccgctgggg ggagtatctg cccggctggc acccctggga ggactaccgg
    15661 gtgtcgtacg ccgcccgcgc ggacttgggc ggcggcgtga cgaatacgct ctcacaccct
    15721 tttgactatc tgcgctggat gctgggggaa gtcgtttcgg tgcgcgcgga aacaagcaag
    15781 ctgagcgacc tggagctgga cgtggaggat aactccgaag cgctgctgga gtttgccaac
    15841 ggcgcgcgtg tgtccgtcca tctggattac tatcaacagc cggccagcca tactctggaa
    15901 ttgacctgtg aacgcggcag aatcctatgg gataacgccg acggcagcgc gcgcctgttg
    15961 gcagcggacg gagagagtaa ccggcagatt atcccgccgg agggttttga acgcaacgat
    16021 atgttcctgg cggagatggc ggattttatc cgtctgtgcc gcggcgaaaa attcgaatat
    16081 tgttcgttgg aggacggcat ccgcgtccag cagattgtgg aagcggtccg ccagtcccac
    16141 gcgctggaag gccggcgcgt cagcctggag tgagggcgga aggcgccctg gtggtaaaat
    16201 ctaaacggaa tttcacagga aaaaacaaaa aaatggaaca aaacgtcctg aataaattcg
    16261 acatgagcgg gcgggccgtg ctggtgaccg gcgcgggagg cctgctgggc aggcagttct
    16321 cgctggcgct ggcacaggcc ggcgcgagcg tgatgctggc agacctggcg gaaaacctgg
    16381 cgcaggggca ggcgcagcat atccgctctc tgggtctgag cgcggaagcc ctgcgcgtgg
    16441 atgtgatcga cccggactcc acccaggcga tggtggcaaa gaccctggag cgttttggtc
    16501 gcctggatgt gctgattaac agcgcggcca tggacccgaa attcgacccg cagcaccaga
    16561 gccagcaggc cgccaacgcc ttcgaaagct attccctcga aaactggcgc aaagcgttgg
    16621 acgtgaacct gaccggcatg ttcctggcat cccaggcggc cgtagcgccg atgctcgcgc
    16681 agggggccgg cgtgattgtg aatatctgtt cgacctacgg cctgaacgga ccggaccagc
    16741 gcatctatga gcgcccggat ggcagccgtt ctttcaaacc ggtctattac tccgttacca
    16801 aggccggggt gctgggtttt acgcgctacc tggcggcgta ttacgccggc aagaacatcc
    16861 gtgtgaacgc gcttaccccc ggcggcgtgt acaacgggca tgacgagcac ttcacccaat
    16921 cctattccgc caaaaccatc ctggggcgta tggcgaacct ggacgagatg agcgcggcaa
    16981 tgctcttcct ggcatcagac gcgtcatcct acatgaccgg cagcaacctg gtggtcgatg
    17041 ggggctggac ggcatggtaa agcagcccga agtgctggcg ctgatcccgg ctcgcggggg
    17101 ttccaagggc attccgcgca aaaacatccg cgaatttgcc ggcgctcccc tgattgcctg
    17161 gagcattgcc gccgctctgc gcgccgagac ggttacgcgt gtgattgtct cgacggatga
    17221 cgaagaaatc gcggcggtgg cgcgggagtg gggcgcggaa acgcccttct tgcgcccggc
    17281 ggaattggcg caggacgata ccgccgatta ccccgttttc cggcaagccc tggactggct
    17341 ggccgcgcac gaggattacc gccccgaggt ggtcgtgcag ctgcgcccga cctcaccggt
    17401 gcgccccctt catctggtcg acgatgcggt caggctgctg ctcgcgcatc ccgacgcgga
    17461 ctgcgtgcgt ggggtggtgc cttccgggca gaacccttat aaaatgtgga agatagacgc
    17521 cgaaagcggg cgcatgctgc cgctgctgca ggtggaaggc cttgccgagc cgtacaacgc
    17581 cccgcgccag agcctgccaa aaacctactg gcagaccgga cacatcgacg ccatccaggt
    17641 cagcacgatt ttggaaaaag gttcgctcac cggtgatgaa atttggccgc tgatgattga
    17701 cccgcgctac actgtggata ttgatacgcc cgcggattgg gagcgttacg aacgcctgct
    17761 gcaggaccca cacatccagg cggtcgaccc tctggtcagc cggcgcgcgt ttccagcccg
    17821 cgtacgcctg ctgctgatgg acttcgacgg ggtcttgagc gacgatttgg tgctcaccga
    17881 ccaggacggg cgcgaatcgc tgcgctgcag ccgctcggac ggctttggct tgagcctgct
    17941 gcgcgaaaaa acggatattc aggcggcggt gctttcccgc gaggaaaacc cggtggtcag
    18001 cgcgcgctgc cgaaagctca agctggaagt ttttcagggc gtccggcaaa aagaccaggc
    18061 gctgaaagcg ctcattcagg cgcgcggatg gaaccctgcg gaaatcctct atctgggcaa
    18121 cgatctgcct gacctgccag ttttacccct ggtcggctat tttgctgccc ctgccgacgc
    18181 gcatccggac gtattacggc gggcggattt aatccttaaa catcggggcg ggcgcggcgc
    18241 agtgcgcgaa atctgcgaac gcctgctttt ggaaacacac aaaacaaacc tggagacact
    18301 atgaaccgcg aagtgacaat tggaaaccgc aaggtcgggg acggacatcc ggcatttatc
    18361 attgcggaaa tcggaattaa ccataacggc agcctggagt ttgccaagca gatgattttt
    18421 gccgcgcatc aggccggcgt ggacgcggtg aaattccaga agcgcacccc cgaactgtgc
    18481 gtaccggaac accagcgcag ccagatgcgt gagaccccct ggggctatat cccctacctg
    18541 gaataccgct acaaggtgga gtttgggcag gaagactatg aggaaatcga ccgctactgc
    18601 aaagagctgg gcatcgcctg gctggcttct tcctgggata tcccttcggt ggagttcatg
    18661 gaacagttca acccgcccgc gcataaaatc ccgtccgctt tgctcaccga ccacgccctg
    18721 ctgcgtgcct accgcaaaac cggacgtccg ctaatccttt ccaccggtat gtccaccctg
    18781 gaagagatta aagcatcggt cgcgttggtt ggcgaggaaa acctgatcct ctgccacacc
    18841 accagcagct acccctgccc tccggatgaa ctcaatttgc gtatgattca gaccctgcgc
    18901 gaaatgttcc gctgcccgat tggctattcc gggcacgagg taggcctggt gccttctgct
    18961 gtggcggtgg ctctgggcgc ctgcctggtg gaacgccaca tcacgctgga ccgcgccatg
    19021 tggggcagcg accaatcggc atccgtggaa ccgcaaggca ttgaacgcct ggtgaagtat
    19081 atccgcgtta ccgaacaggc gctgggggac ggggtcaagc aggtctatga aagcgaaaag
    19141 tcgtccctga gcaaactgag acgcttcagt cagtaggtat ggcagccaat acgcttgttg
    19201 aacgcgcgcg cagacgtctg gcaatatccc gcgcgcacca gattagtgac cagcgcactc
    19261 gcagtatagc tgagctggtg gcggcgcgcg ccccgcagcc aacaaaggcg cctgtagttt
    19321 tctttaacgc cagcacgcgc ctttccgggg tgagcctgaa cgccgccttt ggcatgctgg
    19381 cgggctggtc cctgcgtttg cagggtgttc cggtggtcaa tttcgtctgt aagcgcggta
    19441 tgacccaatg cgttttgggc accgaccgac gcgagccccc caaagccccg ccctgcgcgg
    19501 tgtgcatgcg ccagtcgcgc gtgctctacc acggcggcga aaatgtcgtg tttgagtttc
    19561 agcctgatgc cgccctggac gctgcgctgg agagcctgga cctggcaggc ctgagcgcct
    19621 ttacctggca gggcatgccc cttggggagt tggttttgcc cagcgcgcgc tggatattgc
    19681 gccggcacca cctgcacgaa gaccccacca cgctgcgcat cctgcgggaa tacatccgct
    19741 ctgcctggtc ggtgggcgcg caattccaca aactgctgga cgaggtgcgg ccggccgctg
    19801 tagttgtttt caacgggatg cagtacccgg aggcgtccgc gcgctgggtc gcgcgccaga
    19861 aggggctgcc ggtctactcg cacgaggtcg ggctgcgccc catgagcgca tttttcaccc
    19921 cgggtgatgc cacggcttac ccgctggcgc tcccggaaga ttttgagctt gacgcggacc
    19981 aaaacgctcg tctggatgct tacctgcaga accgctttca aggcaacttc atcatggccg
    20041 gcgtccgttt ttggccggag atgagcgcgc tggacccgga gttcctgacg cttgccaaag
    20101 gcttcaggca gatagtgccg gtttttacca atgtcatttt tgataccagt cagccgcacg
    20161 ccaacgttct ctacgaggac atgttcacct ggctggacga agtactggaa acagcccggg
    20221 cgcacccgga tacgctcttt gtcatccgcg cgcacccgga cgaagcccgc aaaggcaaag
    20281 ccagcgagga atcggtggcg gagtgggcgg aacgccggca ggtggagcag ctcccgaacg
    20341 tccgcttcat ccggccggag acctacatca gctcctacga tttgattcgc atggccaagt
    20401 tcgtcatgat ttacaactcg accatcggtt tggaagcgtc cattcttggt gcgctggtgc
    20461 tggctgccgg acgttcgcgc tacagtcagg cagataccgt gcgctttcct gccagccggc
    20521 aggaatacca gcgcgagctt gaggcgctgc tgcttgcgcc ggaggcgcgc gcccccgaac
    20581 gctaccgcca aaacgcccgc cgcttcctgt actaccagct ttaccgttcg tccctgccct
    20641 tcgatgcttt cctggaagaa gaccaggttt gggcgggtta tgtgcgcctg aaagactttg
    20701 aaccgcgcgc cctgctgcca gagaactccc cgacgctgcg tgctatttcc gagggattgt
    20761 tcgcaggcgg tgatttcctg ttgaaggagg agtagatgag cctggaaaaa attgttgcgc
    20821 tggtgcccat gcggcatgcc tccgtgcgcg tgcccggcaa gaactaccgc ccaattgcgg
    20881 gcaaaccgct ctaccatcac atcctggaaa cgctgctggc ttgcccggag atttcgcagg
    20941 tggtggtgga cacggacagc ccgctgattc tggacggcct ggcaaaagac ttccctacgg
    21001 tggcggcctt gctgcgtccc gagcatttgc gcgcggatac catccccacg aatgaaatct
    21061 tgctgcacga caccgcccag gtccccgccg acctgtactt gcagacccac agcaccaacc
    21121 cgctgctgcg cacgcagact gtttccgcgg ctattcgcgc cctccgggag gtctacccgg
    21181 cgtacgactc gctctttggc gtgacgcgct tgcagacccg cctgtgggac gagcttggca
    21241 gggcgattaa ccacaacccg gctgtgctgc tgcgcaccca ggacctgccg cctgtatatg
    21301 aggaaaactc ctgcatctac ctgttcacgc gcgaaacttt ggcgcaaagg cgcaaccgtc
    21361 tgggtgaacg cccgctgatg ttcgaaatcg cggccgaaga agcctgggat atcgacgaag
    21421 aaatcgattt caccatcgcg gagggattgc tccgcgcgcg aaaggaagca aaatgaaaga
    21481 cgtcctgatt tctgccccgt atatgctgcc cttcctgccg cgcttcacgc cggtgctgga
    21541 acaattgggc atgcgtgtgc tactccccga ggtgcatgag cgtctttctg aggaagaact
    21601 tctggcgtat gccggccggt ttgacggtac tatctgtggg gacgatcgct atacccgccg
    21661 cgtgatacag gcctgcctgc cgcgcctgaa agtcatttcc aagtggggca ccggtatcga
    21721 ttccatcgac caggaagccg cggcggaatt cggtgtgagc gtgcgcaata cgcccaatgc
    21781 cttcaccgtc cccgtcgcgg agagcgtgct tgggtatatg ctcaacttcg cgcggcactt
    21841 cagcgccatg gacagcgcga tgaaagccgg cgaatggcag aaaatacccg gctacacgct
    21901 cagcgagaaa accctgggcg tggtcggcgt gggcaacgtg ggcaaggccg tcctgcgccg
    21961 tgcccgcgcg tttgggatga aattgcttgg caacgatatc attgaaattg ccccggactt
    22021 tctactggaa aacagcgtgg aagccgtgcc tttgcgcgaa ctgctggaac gggcggactt
    22081 catcagcctg aactgctcgc tcaccccttc gtcgtaccat ctcatcaacg cgcaaacctt
    22141 gagcctcgtc aagcctgggg cgctgctgat taacacttcg cgcggacctg tggtggaaga
    22201 acccgctctg gtgcgcgcgc tgcaagcagg gcggctggct ggcgcggcgc tggatgtctt
    22261 tgaggtggaa cccttgcccg cagacagccc gctcctgacg atgaagaacg tcttgctggc
    22321 cccgcacaat tccaactcca gcccgtttta ttgggaacgc gtgcactgga acagcatccg
    22381 caacctggcg ctgggtctgg accttcctgt cggggatatc gccagcatca aggccttgga
    22441 aaagcaagcc tgaaaggaaa aatagcatga ccgaacagtc acaacgcgtt gttttgataa
    22501 ccggcgctgc cggtggggtc gggcaagctg ccgtgaacca tttcttcgcg cggggttggc
    22561 gcgtcatcgg cgtggaccgc aagccggtat atgctcgctt tccagccgac ggcttgttca
    22621 tccaggccga tctcgcgctg ccgcaagagg tggagcgggt ttaccgtgag gccgccgaat
    22681 ttacccccaa gctggatgct ttggtcaata acgctgcctt tcaggtcacc aagccgatta
    22741 gccagacttc ggttgaagag tgggaccagg tgatcgccgt caacttacgc tcagctttct
    22801 tcggcgcgcg ctatggcttt gcgctgctgg ctgctgaggg cggcggcgcc atcgtcaacg
    22861 tggcttcggt gcacgccgtg gcaacttcac ccaacattgc cgcttatgct gctagcaaag
    22921 gcggattggt cgcgctcaca cgggccatgg cgatcgagtt tgccccagct aatattcgcg
    22981 tgaacgctat tctgccgggc gcggtggata cccccatgct cgcggcgggc ttcaaccgcg
    23041 ggcacttaac ccagggcagc atcgagcaac agaaagcgga gctggcgcgc aaaaccgtta
    23101 atggccgcat cggccagcca cacgagattg ccagcgtgat ttacttcttg gctgataaca
    23161 cgcaatccag ttatatgact ggtcagtcag tggtggtgga tggtggtgcc tctgcacgcc
    23221 tgagcacgga gtgagcgcat gcccgaaaaa acagatttga accttccacc gcccttgatg
    23281 gagaccgccc gccgactcta tgacggcgcg cgcaggctgc ccgaacttcc cgcggcgtat
    23341 ttgcacccct ggcggcggga atccattcgc aggctgagct tgctaaggga cgcgcacaaa
    23401 ggcgaacgct gttttttgat tggcaacgga cccagcctga agcgcaccga cctctcgctg
    23461 ctcaggaacg agttcagttt tggcttcaac cgcatctttc tggcggcgga agagctgaac
    23521 tttacgcctt cctgcctggt atccatcaat gacctggtca tcgagcaatc cgcgccggaa
    23581 ttccgcgcgc tggagatgcc caaatttttt gcctggcgct cgcaccgctg ggtgggcatg
    23641 gatgcgcaca cccactacct ctacaccagt tacaccacgc caaaattcgc gcgcgacgtg
    23701 cgggggcggg tgtgggaagg cgccaccgtc acgaacgttg ccctgcagtt ggcgtatttc
    23761 atgggcttca gcacggtcat tctggtcggc gtggaccaca gctttgcgac ccagggcagg
    23821 cccaacacta cagtgcagtc cgagggagac gaccccaacc acttttccag cgcctacttc
    23881 ggcaaaggct tccgttggca gctgcccgac ctggagacct cggagctggc ttaccgcatg
    23941 gcgcgcaagg cttacgaggc ggacggccgg caggtattgg acgcgaccat cgcaggtaag
    24001 ctggaggttt tccccaaggt tgcatatgag agcttgttcg ggtaattgct cccgcccgct
    24061 tgttcatctg aacacttctt gtcctcctga gcgcagcgaa ggtcgtaggt tgggtggagc
    24121 gccagcgcaa cccgataagc cttggccggt gggcccgtgc atcggtcttt gggttttgct
    24181 cccgcgctct ggacccggtc tccatgctgg gaggccggta gtcgtaggtc gggtggagcg
//
LOCUS       DAOQAD010000020        23544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_2166_length_23544_cov_6.610158, whole genome shotgun sequence.
ACCESSION   DAOQAD010000020 DAOQAD010000000
VERSION     DAOQAD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 23544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23544
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2166_length_23544_cov_6.610158"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(484..2070)
                     /locus_tag="PKL60_03025"
     CDS             complement(484..2070)
                     /locus_tag="PKL60_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNW95140.1"
                     /translation="MKKTLFVSLILILALNLSGCSAPAAQQTAIAEQTRSAPLVTPEP
                     GEETAAEPVILFADPVLEGMIRGAMGKPDGSISAEEAQAVTRMDFSNAWSQHLPQDTL
                     IHALGGLEAFTNLESLDLSDQAISDISVIQGLTKLTYLSLANNPVSDVSPLAELTNLK
                     VLILSKSQAQDYSALASLVNLQVLLLDNSTITDLTPLSDLTNLNTLYLANAPAEDLSP
                     IENIYPALAKKDFKIPSTLVDLGFSMDHNNHEAIFDSEEASFTINHEAWGMPSREDNL
                     NIVRMSLYLNGENKASIGYYGIHKVYVCQMVKEGQPQVNYLYNPVDGSFNISPEDRPN
                     AEQMIRAALDVMDGEDALFAPIRFYNDTIQKTFHMTAAALYALPFEPPTLKSLGFFPD
                     KPNAVCFYEQRGERDYNLEIHRPEWGEKEYDVRFFTPLSNDYRIVITYNINEKKFNVG
                     VDDNFGGGGSFEFSSDTKNHVDLWCSDQEMTVEEYFIKAYDDPAIEDVYLHSIEMMRQ
                     YFIERFGLTMEELFSLPGCE"
     regulatory      2573..2681
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2754..3980
                     /gene="metK"
                     /locus_tag="PKL60_03030"
     CDS             2754..3980
                     /gene="metK"
                     /locus_tag="PKL60_03030"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNW95141.1"
                     /translation="MTTTFMKSPKFLFTSESVTEGHPDKICDQISDAVLDEALRADPS
                     SRVAIETVTKTGFVLVTGEMTTNTYVDIEKLVREVVNGIGYDRGKKGFDGSTCAVMVA
                     VSAQSPDIARGVDQNGKTVPVEYSEEYINAGGAGDQGMMFGYACDETEQLLPLPIYLS
                     HKLCRRMAEVRKNGTLKWMYPDGKSQVTVEYHYGKPARIDTVLISTQHADKLAGKTVT
                     NEQIRADLIEYVIKPVLPAELVDDKIKIYTNPTGRFVVGGPLGDSGLTGRKIIVDTYG
                     GMGRHGGGCFSGKDPTKVDRSGAYAARWVAKNVVAAGLASRCEVQIAYAIGKAEPLSV
                     NVETFGTGVIDDDKIAAAISKVFDLRPRAIIHQLDLLKPIYHQLAAYGHFGRDDLDLS
                     WEKTNKTEELRQAAGL"
     gene            complement(4183..5076)
                     /locus_tag="PKL60_03035"
     CDS             complement(4183..5076)
                     /locus_tag="PKL60_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95142.1"
                     /translation="MNSRQDTLPKKDNEALCAYLDGVMTGEERQKFETRLSKSLRLQK
                     ALKEYTQLRLAVRSLPCQPAPRNFTLTREEARAIKRQPVLYPAFSYASLASVLILALL
                     FTSEFIFKTYSAPMAREVPSEPALMTVPSVESTAQAGQPQIFTWGSAYGMGGGGAGGI
                     GVGAPHTAYLPGGGYSMDTTIAEGAAEEPAPPEETLPSEAGGGGLLETPMAETPEAQV
                     FSVETSTEPVEPLIFGVRESEAGQIVSSYPPAQAKSAPETAADSQAEASRALVPDYLK
                     IGLLTAAVAFALAALVFYLKR"
     gene            complement(5073..5681)
                     /locus_tag="PKL60_03040"
     CDS             complement(5073..5681)
                     /locus_tag="PKL60_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNW95143.1"
                     /translation="MSEQDLIELAVSGDLDAFNRLVLQYQDAAYNTAYRIMSDSAAAA
                     DATQEAVISMYRKLNTYRGGSFKSWFLRIVTNACYDELRRLQRRPSVPLEPENDEGEL
                     MDSSSWMEDDTPEPDEAASARELENAIEHCLENLEEKFRVVMVLVDISGEDYESVSAI
                     INRPMGTVKSRLARARQKMQDCLRAFGELLPEKFRLKDEETE"
     gene            complement(5760..7286)
                     /gene="lysS"
                     /locus_tag="PKL60_03045"
     CDS             complement(5760..7286)
                     /gene="lysS"
                     /locus_tag="PKL60_03045"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNW95144.1"
                     /translation="MLQDNFSELENVRLEKLLRLRSEGIEPYPTTTRRDASTAAAVQA
                     FESAEAAGSSEAIKVVLAGRLRAIRNMGKLSFAHIEDRDGKVQLLFRVDEIGEERLQA
                     FSRDFDLGDFIEAEGTMFRTRRGEVTLLVNSFRMLAKALLQLPADKDEVVDGKTVRHA
                     TLTDPETRFRQRYVDLAVNPEVREVFRVRTAAISALRKFLDELGFLEVETPILQPIYG
                     GAAARPFVTHHNELDQDLFLRISFELYLKRLVVGGLERVYEIGRDFRNEGISYKHNPE
                     FTQLEFYMAYADYRSIMEITEQMVSNVAQQVLGTQIIHFDGHEINLTPPWKRLEIRQG
                     LLEQTGVDISEYQTAADLSAVLREKGIQHNPNAPRGKIIGDMMEQFLEPILIQPTFLY
                     DYPRDISPLAKNKPGDPQTVERFEGFVAGMELCNAFTELNDPLEQEKRFLELGRAYAE
                     GDEERHPMDEDFLRALAYGMPPTGGFGMGVDRFVMLLTGQHNIREVLLFPPLRDTNQA
                     "
     gene            complement(7355..7819)
                     /gene="greA"
                     /locus_tag="PKL60_03050"
     CDS             complement(7355..7819)
                     /gene="greA"
                     /locus_tag="PKL60_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNW95145.1"
                     /translation="MPISYLTREGFDKLQAELDELRNVRRNKIAVRLQEAMEDGELIE
                     NAEYEDAKNEQAFVEGRIKDLELLLATARIIDEGENEQGFIQVGTTVTIQEEGYDPET
                     YMIVGPAEADPLLGRISNESPIGRALINHKVGETVKVDAPGGVYTVVIIKTS"
     gene            7960..8352
                     /locus_tag="PKL60_03055"
     CDS             7960..8352
                     /locus_tag="PKL60_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95146.1"
                     /translation="MRPPPEWNEPGISDIIYTGTGLERAMSLIDILLGEEAIRSLPAE
                     QKQEVKELINQLIVIGKQDDFLSLVPGGPFDHQCHHRQAREIGSRLNALGGLDLMWAV
                     RTTIRRKLKDVAAEHLDHCWKDIGGWQP"
     gene            8439..8843
                     /locus_tag="PKL60_03060"
     CDS             8439..8843
                     /locus_tag="PKL60_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNW95147.1"
                     /translation="MNPTRASKQVVTALRAPKAIGPYSAAIACRGFVFVSGQLGIHPE
                     SGNLMEGVEAQARQALRNLSAVLEAAGCDLSAVVKTTVFLKDMADFALVNSVYAEFFT
                     RDFPARSAVQVAALPKGGLVEIEAIAAAPQAE"
     gene            8882..9877
                     /locus_tag="PKL60_03065"
     CDS             8882..9877
                     /locus_tag="PKL60_03065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW95148.1"
                     /translation="MLPKVSVIIPCRNEERTIGLLLEALLKQSFPREALEVIIADGMS
                     TDGTRKAIADFQASHSDLRLRVADNPKGSIPAAVNAGIRAAEGELFLRLDAHSMPHPE
                     YIERCYAALTQGVADVVGGVWDIQPQEDTWIARAIAAAAAHPLAVGDAQYRHGKQAGS
                     VDTVPFGAFKRELVQRVGMYDESLLTNEDYEFNTRVRQSGGKIWLDPQIRCVYFSRRN
                     LKELASQYWRYGFWKAQMLKRYPGSLRLRQALPPLFVLGMALLLLLGLVWQPSLWVFL
                     GAAVLYLAVLVGAGIPLAAHKKDAALIIGFPLAAATMHFCWGAGLLSGLVTKNTR"
     gene            9896..11314
                     /locus_tag="PKL60_03070"
     CDS             9896..11314
                     /locus_tag="PKL60_03070"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNW95149.1"
                     /translation="MQEKNTSPRLRLQPSEHRFILILGDIIAAVLALFIALYLWSAGD
                     TWMNFSLEFLRTRPDSWFYFLPVIWIALMVDNYDLNKAGSLKLTLRAIGLAILVASML
                     YLIVYFTMPPKTLPRRGVAYFIIFVSILTLLWRVIYVRIFSAAIKSRRLLVIGAGKAG
                     SALVNVLADQVQPPYNLLGLIDDDPEKLGTKIRNYQVLGDHSQLLTLVRELGVTDLIL
                     AISGQMNHGMFQNLLTAQEKGLQISTMAQVYEELLGRVPINLLDSEWVVRSFVDRAQL
                     GGIYRLLKRLLDVILTIIALAVFIVLFPIIAVLILIDSGRPVIYSQERLGRGGEPYRI
                     YKFRTMKNKADMEKEALVTAYNDARITRLGKFLRKVHLDELPQVINVMKGEMSWVGPR
                     SERSELVAVFQQEVPFYRARMLLKPGITGWAQIHQRYAETIEETAVKLEYDLYYIEHA
                     NLLMDISIILRTFGSVLGFKGR"
     gene            11321..12334
                     /locus_tag="PKL60_03075"
     CDS             11321..12334
                     /locus_tag="PKL60_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNW95150.1"
                     /translation="MRRVYMEDADWDELLESFPQAHLLQTAGWAEFKAGFGWSSARVR
                     SGDACAQLLFRSLPLGLSIAYLPKGPVGANWEALLPQIESLCRARKAVFLQVEPDILE
                     PLPENLLQRAFPDFRREEHTLQPRRTILIDLTRSEEELLAGMKQKTRYNIRLAEKKGV
                     QVRLSSDLRGFYRMMQTTARRDGFALHDISYYQQAFQVFEPRGKCALLEALLDNQPLA
                     YLMVFLHRERSWYFYGASDDESRNLMPTYLLQWEAMRWAKARGAASYDLWGIPDEDED
                     VLERDFTSRSDGLWGVYRFKRGFGGVVARSAPAFLKVYRPLIYKAYQIYRAQRQAGLD
                     AAS"
     gene            12321..13433
                     /locus_tag="PKL60_03080"
     CDS             12321..13433
                     /locus_tag="PKL60_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNW95151.1"
                     /translation="MRLLELNSARDWNNILLQLPAPHLLQTYEWGQNKIQNGWTPRHY
                     IWEQDDKISAAALVLSRQVSLGLPGLRLEVLYCPKGPTLDWQDEQLAARVLDDLQALA
                     ARRRAAFIKIDPDVELGRGVPGAADARENPQGLAFQRELERRGWVFSADQIQFRNTVL
                     IDLTRGEEEILAAMKQKTRYNIRLAQKKGVRVRAGKENDLPSLYAMYAATAVRDDFVI
                     RHEAYYLKLWGDFMRSGMALPLIAEVEGQAVAGLILFHFAGVARYLFGMSTERARELM
                     PNYLLQWEAIRTSRSLGCRAYDLWGAPDIFDESDTLWGVYRFKEGFNGLTLRHLGAWD
                     YTSRPALYRLYTRALPKLLDLMRARGKDANRKRISL"
     gene            13430..14392
                     /locus_tag="PKL60_03085"
     CDS             13430..14392
                     /locus_tag="PKL60_03085"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HNW95152.1"
                     /translation="MIQRIPLAHLPTPIENLAGLTRLLGGPQLLIKRDDLTGLGMGGN
                     KTRKLEFLAADALDKGARMLISTGAPQSNHCRQVAAAAARLGLGCLLVLAGDDPGARQ
                     GNLLLDALSGAQLIFVPKARRDAALQEQFERARAEGQEPYLIPYGGSNALGAQGYVQA
                     MRELKEQKCEPDWIVFASSSGGTQAGLVSGARQTGFRGRILGISVDEPARVLQKRVAA
                     LAQEAAALNGEKISVAPEEVLVNDGYCQAGYGVLQPAEVEAIRSFARSDGILLDPVYT
                     GRAAAGLLDLIRTGFFHRDETVLFWHTGGTPALFAEPYAGILSQ"
     gene            14768..15646
                     /locus_tag="PKL60_03090"
     CDS             14768..15646
                     /locus_tag="PKL60_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95153.1"
                     /translation="MDIRSTVLTLGILTAIGALWLAISAARQIRRGKRIPFFFKRRKL
                     FEQAASMVIAAIALAFFAFASFRWGEDLAYRYFPPTLTPSLTPTITLTPTITLTPSIT
                     PTPTITNTPSITNTPAIPQDVLDQFESIVTPDANSVFSSLVFSEKVDENFQPVEPAQT
                     FENPLTVIYGTYSYNFMMDGVQWTEMWYRGTTLLKLETGRWEGGSGGYALTSLSLPAE
                     EWLPGDYQVQFFLGNQWLMSGSFSVSGNPPTPTLTPSPTFTRTPTNTPTSTRTPVPTR
                     TPAPTSTPRPSATARP"
     gene            complement(15864..16832)
                     /locus_tag="PKL60_03095"
     CDS             complement(15864..16832)
                     /locus_tag="PKL60_03095"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNW95154.1"
                     /translation="MENKKSIFPFFKTSVKSDFIISDDVERAKRNGRQIVALESALIT
                     HGVPWPENLNIARSMEKAVRDNRAYPATIAVQEGQLRVGLNAAQLEMLAGAREARKCS
                     LWDLSGAIVRGGSAGTTVSCTLYAAERAGIKVLATGGIGGVHRGAAYDISTDLRQLSR
                     SPLVVVCSGAKAILNLPATLEMLETSGVPVIGYQTDDFPAFYSRESGLKTPLRAETPQ
                     EVANIANTHWKLGFHTSVLVVVPPPAESALPREHMEALITTALQQAEAEGVRGSAVTP
                     YLLSKINELSGGESLRTNLALLSNNARIAAQIAVVLPEEKTDWRYI"
     gene            16966..18687
                     /gene="recJ"
                     /locus_tag="PKL60_03100"
     CDS             16966..18687
                     /gene="recJ"
                     /locus_tag="PKL60_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNW95155.1"
                     /translation="MENSTLTPTPKRWLVSPLIPPNVSSELEEFPSLLRQLLYNRGIT
                     DYQAARAFLDCAPGADTNPFLMKDMQAAVEILRQAVLENAPIAIYGDYDVDGVTSSAL
                     LYEFLSRLGQTPRVYIPSRFDEGYGLNLDAVEQLHREGITLMITVDCGIRSIAEIALA
                     KSLGMRVILSDHHQPGAQLPPADAVINTRQAGDPYPYKQLAGVGLAYKLADAYLIKYP
                     QTGLDAQDWLDLVALGTVADLAPINGENRALVRAGLDQMRATRRQGLYSLAQVSGINL
                     ENVNSSNIGFSLGPRLNAAGRLDSAMAAFELLTSTDIFSAGMLAQQLDAQNAQRKDLT
                     LRVQEQAIQSALQADPDSPVIFAASPDFNEGVVGLAASRVAEQLYRPAIIGSRGETNI
                     VASCRSIPGFNITNALDECADLLVRYGGHSMAAGLTVSLEKAPELLARLNAIAREALQ
                     GMELVPVLGIDREIRLEHMHPEFVPQVFEAVEKLEPTGRENPEALFCSRGLRVAGARA
                     VGDGQHLKLTLQAGRNSFDAIAFKQGYWLNQLSDSIDIAYTFELNSYMGRKTLQLNVK
                     DIHPAQG"
     gene            complement(18699..19796)
                     /locus_tag="PKL60_03105"
     CDS             complement(18699..19796)
                     /locus_tag="PKL60_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNW95156.1"
                     /translation="MNAKPTDSFPYPRRKFARSFLRSTARLLLTLLTRLKVRGMENFP
                     RGGPLILAGNHVGVLEAVLMTALSPRQVEFLGTGDIPLDPNYARIVDAYGLIPINRGN
                     LDRDAIKKSVDVLRQGGVLGIFPEGGIWDPAHMEAQLGVALISQRANAPVLPIGFGGM
                     RGALGSALKFRRPRLEMRVGELIPPAQIPEGADRRQALQEYASTVLQRITALIPASER
                     TDLPESVEYALTLEPISPSPNALPNLPAERGQAFAHLLFSPVLLDALHRNLKLPVGVW
                     IDPAASVSNAEFSTALSAVLEYLESNPGFFTYRFGMPEGLALGGALSDLRGILASACE
                     SGLALRLGASSRTRYEDGRAEEKFLNFAIVP"
     assembly_gap    20218..20317
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20448..21533)
                     /locus_tag="PKL60_03110"
     CDS             complement(20448..21533)
                     /locus_tag="PKL60_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNW95157.1"
                     /translation="MSHQRISQSKTHRKLILGVTLIFLFSFVLSPQAGFASSSKPTIM
                     VGLIPDVAGVDDNGFNEMAAAGLARAATDFGILPAIYQPASTDEYAAKIGECVSAGST
                     LCITVGWLMGDATMAAAIANPGVNFAIVDMTWDTYPPNLRGISFASEQVGYLAGALAG
                     LMSTSKVIGAIGGLPIPTVTAFMTPYQYGAQWADHSVQVLLEYSYDFGNPPLGEQMAQ
                     DQMARGADVIFAVAGPTGIGVNLETAQAGAWTIGVDVDAYYSTFGGGTVDGHEFLLTS
                     ALKRVDNAVYHTIEEVVGGTFTPGTMINNLENEGVGLAPYHDAAASIPQDVQDEVAAV
                     SQSIINGETDVWEPFYKYSISLPVVMK"
     gene            complement(22155..22227)
                     /locus_tag="PKL60_03115"
     tRNA            complement(22155..22227)
                     /locus_tag="PKL60_03115"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22191..22193),aa:Glu,seq:ctc)
     gene            22413..23384
                     /locus_tag="PKL60_03120"
     CDS             22413..23384
                     /locus_tag="PKL60_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNW95158.1"
                     /translation="MQYRHLGKAGIKVSELSIGSWVTYGKQVDVRAARDILQTAWEAG
                     VNFFDNAEAYAGGQSEIIMGKALKELAFPREQYLISTKIFWGGKGPNDTGLNYKHIIE
                     GVNNALKRLGLEYVDFVFAHRPDPETPMEEVVWGFNQIIQQGKAFYWGTSEWSAAQIM
                     QAYQVAKEYHLRPPSMEQVQYNLLHRRRFEKEYAVLYKELGYGTTVWSPLAGGLLTGK
                     YNDGKIPEHSRATLSGWEWVKDDVLDPEKLKVTRKLAEIAADLGVSMAQMSLAWLLKN
                     PNVSTVITGASRVEQVRENMKAADVVPLLTDEVMARIEAALEGYREE"
BASE COUNT         4841 a         6677 c         6962 g         4964 t
ORIGIN      
        1 ccatacaaga acagtttgta ggttgggtgc agcgacgcag gagcggaacc caacaatggc
       61 tccacaaacc cgctggccaa agcctgtcgg gttgcgctgc tgctccaccc gacctacgat
      121 tcccgcccca ccagacctac gattcccgct ccacccgacc tacgattccc gctccacccg
      181 acctacgatt gtcgctccac ccaacctgcg actgcgtccg gaaaatatcc cgccagtcaa
      241 accttggtgc tgctccggcc aactgaccaa tgcgcatccc gtggtgagga ccatcgtcat
      301 gctgatctgc cgccgaagca tcccggatag ggttcgccat atatcattcc acagaggcat
      361 aaaaggcaga tcgccgcgcc gtggtcctcg cgatgaaaac ttccccgtcg tgcttcagtg
      421 gagccaaaca gcaatttact gctgcgttgg agagggctgc cctaaaaaca gtaccagcgc
      481 tccctattcg cagccaggga gggaaaacag ctcttccatt gtcagaccaa accgttcgat
      541 aaaatactgg cgcatcattt ctatggagtg caggtagacg tcttcgatcg cagggtcatc
      601 gtaggctttg ataaagtatt cttccaccgt catctcctga tcactgcacc agaggtcgac
      661 gtgattcttg gtatcgctgg agaactcaaa acttccccca cctccaaaat tatcatccac
      721 cccaacgtta aacttctttt cattgatgtt ataagttatc acaatgcgat aatcgttact
      781 aagaggcgta aaaaatcgaa cgtcgtattc cttctcgccc cattccgggc gatgaatttc
      841 gaggttataa tcccgttccc cgcgttgctc atagaaacag acggcattcg gcttgtccgg
      901 gaagaagccc aggctcttga gtgtcggggg ttcaaaaggc agggcataca gcgcggcggc
      961 ggtcatgtgg aaagtttttt ggatagtgtc attataaaag cggatcgggg caaacagcgc
     1021 atcttcccca tccatgacat ccaatgctgc gcgaatcatt tgctccgcat ttggacgatc
     1081 ttccgggctg atgttaaagc tgccgtcgac cgggttgtag aggtagttca cttgtggttg
     1141 gccttctttt accatttggc aaacatagac cttgtggatg ccataatatc caatagatgc
     1201 tttattttca ccattcaagt acaaggacat gcgaacaata ttcagattat cctcccttga
     1261 aggcatgccc caggcttcat ggttgatggt gaaggaagcc tcttcactat caaaaatcgc
     1321 ttcgtgatta ttgtgatcca tgctgaatcc gagatccacc agcgtggaag ggattttgaa
     1381 atctttcttt gcgagggcag ggtagatgtt ctcgatgggt gaaaggtctt ctgcaggggc
     1441 gttggccagg taaagggtgt tcagattggt taggtcggac agcggggtga ggtcggtgat
     1501 ggtggaattg tcgagcagga gtacctgcaa attaaccaag ctggcaagtg cactgtaatc
     1561 ctgcgcctgg cttttggaga ggatcaagac cttgaggttg gtcagctcgg caagcgggga
     1621 aacatcgctg accgggttgt tagccaacga aaggtacgtg agtttggtta acccctggat
     1681 aacggaaatg tcgctgattg cctgatcaga caagtcgagg ctttcgaggt tggtgaaggc
     1741 ctccaacccg ccaagagcgt ggataagtgt gtcctgcggg aggtgctgcg accatgcgtt
     1801 gctgaaatcc atgcgcgtga ccgcttgggc ttcttccgcg ctgatactgc catccggctt
     1861 gcccatcgcc ccgcgtatca tcccctccag cacagggtca gcaaacagga taaccggctc
     1921 cgctgcggtt tcctcacccg gttcaggcgt aaccaacgga gcggacctgg tctgctccgc
     1981 tattgcggtt tgctgcgcgg caggcgcgga acagccgctc agattgagcg ccagaatcag
     2041 aatcagcgat acgaacagtg tttttttcat cggagagagc tcctttatgt cagttgaggt
     2101 gaggccgttt ttttcatgcg catgcggaaa gcagcagcga ttcgattcta cctgttctgt
     2161 tcaaaaatca gcgattccac ggcatttctc gtgaacaatt cctcccgctg tctaccaatc
     2221 aaagaaaacc gataggtttc aggactttcc ctgccaaaaa atcccctgcg cctggtgaga
     2281 attgtgattc ctgacgctgt acgacgtaca ggcgcgttga aagaaatccg ccctgcgttt
     2341 tcccttcatc ctcctgcgtg cgcgggtaaa gggcattgca gccgtgcggg tggaagttgc
     2401 ggaaccggac cttggattga ctgccggcgc gtttgaggag ggggaggctc tgcaaagccg
     2461 gggtcagcgc tgcgctccga gcccggaatg tcgcggcagc ttgtatttgc tattgcataa
     2521 aaaactaaag cgtgatataa gaaaaataac aatttaacag ccctaagcgg cgcacttatc
     2581 cagagaggtg gagggaacgg cccgatgata cctcggcaac caccaggtga aaacctggac
     2641 ggtgccaact ccggcagagc agtatttctg gaagatgaga ggcgagccag catgaacaat
     2701 gcccccctct ccgcgagggg ttctttttta ttagaaactg gaggtttcta actatgacga
     2761 cgacttttat gaagtcacct aaatttctgt tcacttccga atctgtgacc gaaggacacc
     2821 cggataaaat ctgcgaccaa atcagcgacg cagtgctcga cgaagcgctg cgggcggatc
     2881 cctcctcccg cgttgcgatt gaaaccgtta ccaaaaccgg ctttgtgctg gtgaccggtg
     2941 agatgaccac gaatacctac gtggatatcg aaaaactggt gcgcgaggtg gtcaacggca
     3001 tcgggtacga ccgcggcaag aagggcttcg acggctccac ctgcgccgtg atggtggcag
     3061 tctccgcgca gtctccggat atcgcgcgcg gcgtggacca gaacggcaag accgtgccgg
     3121 tggaatacag cgaagagtat atcaacgctg gcggcgcagg cgaccagggc atgatgtttg
     3181 gctatgcctg cgacgaaacc gagcagctgc tgcccctccc gatatatctg tcgcacaaac
     3241 tctgccgccg gatggcagaa gtgcgtaaga acggcacgct taaatggatg tacccggacg
     3301 gcaagagcca ggtgaccgtg gaataccact acggcaagcc cgcccgcatc gataccgtgc
     3361 tcatcagcac gcagcacgcc gacaaactgg cgggcaaaac cgtcaccaac gaacaaatcc
     3421 gtgcggattt gattgaatac gtgattaaac ccgtgctccc tgccgaactt gtggatgaca
     3481 aaatcaagat ttacaccaac cccaccggcc gctttgtggt cggcggaccg ctgggtgatt
     3541 ccgggctgac cgggcgcaaa attatcgtgg atacctacgg cggcatgggc aggcacggcg
     3601 gcggctgctt cagcggaaaa gacccgacca aggtcgaccg ctccggcgca tatgccgcgc
     3661 gatgggttgc caagaatgta gtggctgccg ggctggcttc ccgctgcgaa gttcagattg
     3721 cctatgccat tggcaaagcc gaaccgctga gtgtgaatgt ggaaaccttt ggcaccggcg
     3781 tgattgacga tgacaaaatt gccgcggcca tcagcaaggt gttcgacctg cgcccgcgtg
     3841 ccatcattca ccagttggac ctgctgaagc ctatctacca ccagctggct gcttacggcc
     3901 actttggacg cgacgacctg gatttgagct gggagaagac gaacaagacc gaagaactgc
     3961 gccaggcagc cggtctttaa gcagcgcttt caaaaaaacg gggctgtcca aaaagaatgg
     4021 gttttccttt tgagccagcc caaagtggga gaacctgcct gtttaggccc cgttttgata
     4081 gcgcaaatcc gtaaacgccc atataaacgg gcgtcgagtg ggaaatcaaa tagggccgtc
     4141 cgacttttag gacagccctg tttggttatc aaggggggca tgtcagcgtt tcaggtagaa
     4201 caccagcgcc gccagcgcga acgcaacggc agcagtcagc aggccaattt tcagataatc
     4261 cgggacgagc gcccggctgg cttcagcctg gctgtccgcg gctgtctcgg gcgcgctttt
     4321 ggcttgcgcc ggcgggtaac tggaaactat ctgaccggct tcggactcac ggacaccgaa
     4381 aatgagcggt tcgaccggtt ctgtactggt ctcgacgctg aaaacctggg cttcgggggt
     4441 ttccgccatg ggggtctcca gcagtccgcc gcctcctgct tcggaaggca gcgtttcttc
     4501 cgggggggcc ggctcttcgg cggcgccctc tgctattgtc gtgtccatgc tataaccgcc
     4561 gccaggaagg taggctgtgt gcggggctcc aacgccgatg ccgcccgcgc cgccgccgcc
     4621 catgccgtat gcgcttcccc aggtaaatat ctgcggctgc ccggcttggg ctgtgctttc
     4681 gacgctgggc acggtcataa gcgccggctc actgggtact tcacgcgcca taggcgcgga
     4741 ataggtcttg aagataaatt cactggtgaa gagcagggct aaaatcaaaa ccgacgcgag
     4801 agaagcgtag ctgaacgcgg ggtagagcac tggctggcgc ttgattgccc gggcttcttc
     4861 ccgggtgagg gtaaagttgc gcggagcagg ttggcagggc aggctgcgca ctgccaacct
     4921 gagctgggta tattccttga gcgctttttg caggcgcagt gattttgaca gacgcgtttc
     4981 aaacttctgt ctttcttcgc ctgtcatcac gccgtccaaa tacgcgcaca aggcttcatt
     5041 atcctttttc gggagtgtat cctgcctgct gttcattccg tttcctcatc tttgagacgg
     5101 aatttctcgg gtaaaagttc cccaaaagcg cgcaagcagt cctgcatctt ctgccgcgcg
     5161 cgcgccaggc ggcttttcac ggtgcccatc gggcggttaa tgatcgcgga aacgctttcg
     5221 taatcttcgc cggagatatc caccaacacc atcacgactc tgaatttttc ctcaaggttt
     5281 tccaggcagt gctcgatggc gttttccaat tcccgggcgc tggcggcttc atcaggttcg
     5341 ggggtgtcat cctccatcca ggaggaagag tccatcaatt caccttcgtc gttctccggt
     5401 tcgagcggaa cagaaggccg gcgctgcagc cggcgcagtt catcatagca ggcgttggtt
     5461 acaatgcgca gaaaccagct cttgaacgag ccgccgcggt aggtgttcag tttacggtac
     5521 atggagatga ccgcttcctg ggtagcgtcg gcagcggcag cagagtccga cataatgcgg
     5581 taggcggtat tgtaggcggc atcctggtac tgcagtacca ggcggttaaa ggcgtccaaa
     5641 tcgccgctga cagcaagttc aatgagatct tgttcgctca taaccatcat ggatgcaaga
     5701 cttgcgccag tttgcggcgc cagcctgctc aattcagatt gtacatgatt tccaggcgat
     5761 tatgcctggt ttgtatcgcg taagggcgga aagagaagca cttcgcggat gttgtgttga
     5821 ccggtgagca gcatcacaaa gcggtcgacg cccatgccaa agccgcccgt tgggggcatc
     5881 ccgtaagcca gcgcgcgcag aaaatcctcg tccatcggat ggcgctcttc gtcgccttcc
     5941 gcgtaggcgc gcccgagctc caggaaacgt ttttcctgtt ccagcgggtc gttcagttcg
     6001 gtgaatgcgt tgcagagttc catgccagcc acaaagcctt caaagcgctc caccgtttgc
     6061 gggtcgccgg gtttgttttt ggccagcggc gagatatcgc gggggtagtc gtagaggaag
     6121 gtgggctgga tgaggatagg ctcgaggaac tgctccatca tatcgccaat gatcttaccg
     6181 cgcggcgcgt tcgggttgtg ttgaatgcct ttttcgcgca ggacggcgct caggtcggca
     6241 gcggtttgat attccgagat atccacgccg gtttgttcca gcagcccctg gcggatttcc
     6301 aggcgcttcc agggcggggt caggttaatc tcgtgcccgt caaaatggat gatttgggtg
     6361 cccaggacct gctgcgcgac gtttgacacc atctgttcgg tgatttccat gatgctgcgg
     6421 tagtccgcgt aagccatgta gaattcgagc tgggtgaatt ccgggttgtg cttgtacgag
     6481 atgccttcgt tgcggaaatc gcgcccgatt tcgtaaacgc gctccagacc gcccaccacc
     6541 aggcgcttga ggtacagctc gaagctgatg cgcaggaaga ggtcctggtc cagttcgtta
     6601 tgatgggtta cgaacggtcg ggctgccgcg ccgccgtaaa tcggctgcag aatcggagtt
     6661 tcgacctcga ggaagccgag ctcatccagg aatttgcgca gagcgctgat cgcggccgtg
     6721 cggacgcgga agacctcgcg cacttcgggg ttgaccgcca ggtccacgta gcgctgacgg
     6781 aagcgggttt cggggtcggt cagagtggcg tggcgcacag tttttccgtc caccacttca
     6841 tccttgtcgg cggggagctg cagcagcgct ttggcgagca tgcggaagga atttaccagc
     6901 aaggtcacct cgccgcggcg cgtgcggaac atcgtgcctt ccgcttcgat aaaatcgccc
     6961 aggtcaaaat cgcgcgagaa agcctgcaag cgctcttcgc caatctcatc cacgcggaag
     7021 aggagctgaa ccttgccgtc gcggtcttcg atgtgggcaa agctgagctt gcccatgttg
     7081 cggatggcgc gcagccggcc ggccaggacc actttaattg cttcgctgct gccagcggcc
     7141 tccgcggatt caaaagcctg caccgccgca gccgtggaag cgtcccgcct ggtcgtggtg
     7201 gggtacggct caatgccttc cgaccgtaaa cgcagcagtt tttcgaggcg gacattttcg
     7261 agttcagaaa agttatcttg cagcataagc tttaatcctt cggattaaaa tataccccac
     7321 cagtgtgctg gtggggtact tgggtttgtt ttaatcagct ggtcttgatg attacaaccg
     7381 tgtacacgcc accaggggca tcgaccttga cggtctcgcc taccttatgg ttgatcaggg
     7441 cgcgcccgat cggggattcg ttcgaaatcc tgccaagcag cgggtcagcc tccgccggtc
     7501 cgacaatcat gtaagtttcg gggtcgtatc cttcttcctg aatggttact gtggtgccca
     7561 cctgaatgaa gccctgttcg ttctcgcctt cgtcgatgat gcgcgcggta gcaagcagca
     7621 gttccaggtc cttgatgcgc ccttccacaa atgcctgttc atttttcgcg tcttcgtatt
     7681 cagcgttttc gatgagctcg ccatcttcca tagcttcttg cagccgaaca gcgattttgt
     7741 tgcggcgtac attccggagc tcgtccagtt ctgcctgcag tttgtcaaat ccctcgcgtg
     7801 ttaaatacga tataggcatc ttatgtcctt ttttacgaat cattatggca gtaaaatcaa
     7861 atggcgcctg gaaaaggtgc cttcctgaaa gcgcgggaaa attataccat aaggcggaaa
     7921 atacatcgcg agctctgctt tgctgccaaa gagagcagaa tgagacctcc gccggaatgg
     7981 aatgagcctg gcatctcgga tataatctac acaggaaccg gattggagcg tgccatgagc
     8041 ctgattgaca tccttttggg tgaagaagct atccgcagct tgcccgcgga gcaaaaacag
     8101 gaagtgaagg aactcatcaa ccaactgatt gtcatcggca aacaggatga ctttctgtca
     8161 ttggtccccg gagggccttt tgaccaccaa tgccatcacc gccaggccag ggaaatcggg
     8221 agccggctga acgcgctggg cggcctggac ctgatgtggg cagtccgcac gactatccgc
     8281 cgcaaactga aagacgtggc agcggaacac ctggaccact gctggaagga cataggcggc
     8341 tggcagccct gagcgctttg ccgcctggct gcgggctctc ccgcaaaggg gatgttataa
     8401 taggttctcg ataatacaag catgaaagag gagccgtaat gaatccaacc cgggcgtcca
     8461 aacaggtcgt gacagcgctt cgcgctccta aagccatcgg tccgtactcc gccgcgattg
     8521 cctgccgggg ttttgtgttc gtttccgggc agcttggcat tcaccccgaa agcggaaacc
     8581 tgatggaagg cgtggaagcc caggcgcgcc aggcgctgcg caacctcagc gcggtgctgg
     8641 aagctgccgg atgcgacctg agcgccgttg tgaaaaccac cgtattttta aaggatatgg
     8701 cggatttcgc gcttgtgaat agcgtttatg ctgaattctt cacccgggat tttcccgccc
     8761 gttccgctgt ccaggtggca gctttgccca aaggcggact ggttgaaatt gaagcaattg
     8821 ccgctgctcc acaggctgag taaaccggcc tgattgctac ccagtgaaca aatccccagc
     8881 aatgctgcca aaagtttccg tcatcatccc ctgccgcaac gaagaacgca ccatcggctt
     8941 gcttctggaa gccttgctga agcagagctt tccgcgcgag gcgctggagg ttatcatcgc
     9001 ggacgggatg tccactgacg gcacccgcaa ggccattgcg gattttcagg cttctcattc
     9061 ggatttgcgc ttgcgcgtgg cggacaaccc caaaggcagc atcccggcgg cggttaacgc
     9121 cggcatccgt gccgcggagg gcgagctgtt cctgcgcctg gacgcgcact ccatgccgca
     9181 tccggaatat atcgagcgct gttacgccgc gctgacgcag ggcgtcgccg atgtggtcgg
     9241 cggcgtgtgg gatatccagc cgcaagagga tacctggatt gcccgcgcca ttgccgcggc
     9301 tgccgcgcat ccgctggcgg tcggagacgc gcagtaccgc cacggcaaac aggctggcag
     9361 cgtggatacc gtcccctttg gcgctttcaa acgcgaattg gtgcagcgcg tggggatgta
     9421 tgatgaatct ctgctcacca acgaggacta tgagttcaac acgcgcgtgc ggcagtccgg
     9481 cggaaaaatc tggctggacc cgcaaatccg ctgtgtgtac ttttcccgcc ggaacctgaa
     9541 ggaacttgcc agccagtatt ggcgctacgg cttttggaag gcgcagatgc tcaagcgcta
     9601 tccaggttcg ctgcgcctca ggcaggcgct gccgccgttg tttgtgctgg ggatggcgct
     9661 gctgttgctt ttgggtttgg tctggcagcc ctcgttgtgg gtattcctgg gcgcggccgt
     9721 gctctacctg gcggttctcg taggagctgg cattccactt gcagcccaca aaaaagatgc
     9781 cgcgctgatt attggtttcc cgctggcagc cgccaccatg cacttctgct ggggcgcggg
     9841 tctgctttca gggctggtta ccaaaaacac ccgttaagcg caggagctcg aaaagatgca
     9901 ggaaaaaaac acttcaccac gcttgcgttt acagccttca gagcacaggt ttatcctgat
     9961 tctgggggat atcatcgcgg ctgtcctggc gctttttatc gcgctctatt tgtggtctgc
    10021 cggggacacc tggatgaatt tctcgctgga gttcctgcgc acccgtccgg attcctggtt
    10081 ttactttctg ccggtcatct ggattgcctt gatggtggat aactacgatc tgaacaaagc
    10141 cggcagcctg aagttgacct tacgagccat cgggctggca attctcgtcg cttccatgct
    10201 ctacctgatt gtgtacttta ccatgccgcc gaagacgctg ccgcggcgcg gggtggcgta
    10261 ctttatcatc ttcgtctcga tcctcaccct cctctggcgc gtgatttacg tccggatatt
    10321 ttccgcggcg atcaaatccc gccggctgtt ggtgattggc gccgggaaag caggcagcgc
    10381 gctggtgaac gtcctcgccg accaggtgca gccgccttac aatctgttgg gactgattga
    10441 cgatgacccc gaaaaactgg gcacgaaaat ccgcaattac caggtgctgg gcgaccattc
    10501 ccaactgctg acgctggtca gggaattggg cgtgaccgat ttgatattgg cgatttccgg
    10561 gcagatgaat cacggcatgt tccaaaacct gctcaccgcc caggaaaaag ggctgcagat
    10621 atccaccatg gcgcaagttt atgaggaact gctcgggcgc gtgccgatta acttgctcga
    10681 ttctgaatgg gtggtgcgtt cctttgtgga ccgcgcgcag ttaggcggaa tttaccgact
    10741 gctcaagcgc ctgcttgatg tgattttgac gattatcgcg ctggcggttt tcattgtgct
    10801 cttcccgatt atcgccgttc tcatcctgat tgattccggc agacccgtca tctacagcca
    10861 ggaacgcctg ggccgcggcg gcgaacccta ccgcatctac aaatttcgca cgatgaaaaa
    10921 caaggcggat atggagaagg aggcgctggt aactgcatat aacgacgcgc gcatcacccg
    10981 cctgggcaaa tttcttcgga aggttcacct ggatgaactt ccgcaggtga taaacgtgat
    11041 gaagggcgaa atgagctggg ttgggcccag gtctgaacgc agcgagcttg tggcagtatt
    11101 ccaacaggaa gtcccctttt accgcgcccg gatgctgtta aagcccggca tcactggttg
    11161 ggcacaaatc caccagcggt atgctgaaac aatcgaagag accgccgtaa agctggaata
    11221 cgacctgtat tacatcgagc acgccaacct gctgatggac atcagcatca tcctgcggac
    11281 tttcggttcg gtgcttgggt ttaaggggcg gtagaaactg atgcgccgcg tttacatgga
    11341 agatgctgac tgggatgagc tgctggaaag ctttccgcaa gctcatttgc tgcagacagc
    11401 tggctgggca gaatttaagg cgggtttcgg ctggtcctcc gcgcgggtgc gttccgggga
    11461 tgcctgcgct cagcttctgt ttcgctccct gccgttgggt ctgagcatcg cgtatctgcc
    11521 caaagggccg gtgggcgcca actgggaagc gctcctgccg caaattgaga gcctctgccg
    11581 cgcgcgtaaa gctgtgttcc tgcaggtgga accggatatc ctggagccgc tgccagaaaa
    11641 cctcttgcag cgcgcctttc cggatttccg ccgggaagag cataccctcc aaccgcgccg
    11701 cactattctg attgacctta cccgctcgga ggaagaactg ctggcaggga tgaagcaaaa
    11761 gacgcgctac aacatccgtc tggcggaaaa gaagggcgtg caggtacggc tgagcagcga
    11821 tttacgcggt ttttaccgca tgatgcagac caccgccagg cgcgacggct tcgcgctgca
    11881 cgacatttca tattatcaac aggcctttca ggtgttcgag ccgcgcggca agtgcgcgct
    11941 gctggaagcg ctcctggaca atcagccgct ggcgtacctg atggtctttt tgcatcggga
    12001 gcggtcctgg tacttttacg gcgcgtcgga tgatgagagc cgcaacctga tgcccaccta
    12061 tttgctgcag tgggaagcca tgcgctgggc aaaggcgcgc ggcgcggcca gctacgacct
    12121 gtggggcatt ccggacgagg atgaggacgt cttggaaaga gactttacca gccgttcgga
    12181 cggtctgtgg ggcgtgtacc gcttcaagcg cggctttggc ggggtggtgg cgcgttccgc
    12241 gccggctttc ctcaaggtgt accgcccgct tatctacaaa gcttatcaaa tctaccgggc
    12301 gcagagacag gcaggtctgg atgcggcttc ttgagctgaa ctccgcaagg gactggaaca
    12361 acatcctcct gcagctcccc gcgccgcacc tgctgcagac ctatgagtgg gggcagaaca
    12421 aaattcagaa cggctggacg ccgcggcatt acatctggga gcaagacgac aaaatcagcg
    12481 ccgccgcgct ggtgctcagc cggcaggtaa gcctggggct gcccgggctg cgcctggaag
    12541 tgctctactg ccccaagggg ccgaccctgg attggcagga cgagcagctt gcggcgcgcg
    12601 ttttggatga cctgcaagcc ctggctgcca ggcggcgggc tgcctttatc aaaattgacc
    12661 cggacgtgga gcttggcagg ggcgtgcccg gagccgcgga cgcgcgcgag aatccgcaag
    12721 gcctggcctt tcagcgggaa ctggagcggc gcggttgggt cttttctgcg gaccagattc
    12781 aattccgcaa taccgtgttg attgacctta cccgcggcga agaggaaatc ctggcggcga
    12841 tgaagcaaaa gacgcgctac aacatccgcc tggcgcaaaa gaagggcgtg cgcgtgcgcg
    12901 ccgggaagga aaacgacctg ccttctctct acgcaatgta cgccgcgaca gctgtgcggg
    12961 acgactttgt catccgccac gaagcttact acctgaaact gtggggcgat tttatgcgct
    13021 ctgggatggc cctgccgctg attgcggaag tggaagggca ggcagtcgcc ggcctgatat
    13081 tgttccactt tgccggggtt gcgcgttatc tgtttggcat gtccaccgag cgggcgcgcg
    13141 aactgatgcc caattacctg ctgcagtggg aggcaatacg cacgtccagg tcgctgggct
    13201 gccgtgccta tgacctgtgg ggcgcgccgg atatctttga cgagagcgac acgctgtggg
    13261 gcgtctaccg ctttaaggag ggctttaacg gcctcacctt gcgccacctg ggcgcctggg
    13321 actacaccag ccgccccgcg ctgtaccggc tgtatacccg cgctctgccc aaactcctgg
    13381 acctgatgcg cgcgcgcgga aaagatgcga accggaaaag gatttccctg tgattcagcg
    13441 cattcctcta gcccacctgc ccacgcccat tgagaacctg gcgggcctta cccgcctgct
    13501 gggaggaccg cagctgctca tcaaacgcga cgacctgacc ggcctgggaa tgggcggcaa
    13561 caaaacgcgc aagcttgagt tcctggcggc agacgcgctg gacaagggcg cgcgcatgct
    13621 tatcagcacc ggcgcgccgc agtccaacca ctgcaggcag gtggcggctg cagcggccag
    13681 gctgggtttg ggctgcctgc tggtgctggc gggggacgac ccgggcgcgc gccagggcaa
    13741 cctcctgctg gacgcgctca gcggcgcgca gcttatcttt gtgcccaaag cccggcgcga
    13801 tgctgcgctg caggagcagt ttgagcgagc gcgcgcagaa gggcaggagc cttacctgat
    13861 cccttacggc ggttcgaacg ccttaggcgc gcagggttac gtccaggcca tgcgtgagct
    13921 gaaggaacag aaatgcgaac cggattggat tgtctttgcc agttcgtccg gcgggacgca
    13981 agccggcctg gtatcaggcg ccaggcaaac gggattccgc ggccggattt tgggcatcag
    14041 cgtggacgaa cccgcgcggg tactgcagaa gcgggttgcc gcgctggctc aggaagccgc
    14101 ggcgctgaac ggggaaaaga tttcggtcgc gccggaagag gttctggtga atgacgggta
    14161 ctgccaggca ggctacgggg tgctccagcc ggctgaagtt gaagcaatcc gcagctttgc
    14221 gcgcagcgac ggcatcctct tggacccggt gtatacgggc agggcggctg caggcttgct
    14281 tgacctcatc cggacgggct ttttccacag ggatgagacg gtgctcttct ggcataccgg
    14341 cggcacgccc gcgctctttg ctgagcctta cgctggcatt ctcagccagt aacgccagcc
    14401 gcaaccggcg cgctgcctgt tcttcagttt cccgcaagca accatcggaa tttgcgcttg
    14461 agcggctggc catactgagc tgctggcgaa gcatcccctg accggcaggg agaccgcttc
    14521 gtacttcgcg gtgaggagca aaggcagatt gccacgcccc ctgactgtaa ttcctggcag
    14581 tatttcatac ctgcagcggg ggctcgcaac gacaaacagt ttagctcgcg gtgacgacca
    14641 aacagggggt taatgacaaa cagtttagct cgcggtgacg aaaaaagttc aggatgacag
    14701 agccagcgcc cgcccgcgcg ttcacggcga tactgctata atccagtggg gactaaaatc
    14761 aatgtctatg gatatccgtt ccactgtact taccctgggg atcctgaccg caatcggcgc
    14821 gctctggctg gcaatcagcg ccgcccggca gattcggcgc ggcaaacgca tcccgttctt
    14881 cttcaaacgc cgcaagctct ttgaacaggc cgccagtatg gtcatcgccg caatcgccct
    14941 ggctttcttt gcctttgcca gcttccgttg gggggaagac ctggcttacc gctacttccc
    15001 gcccacgctc accccctcgc tcacgcccac catcacgctc acccccacca tcacgctgac
    15061 gcccagtatc acccctaccc ccactattac caacacgcct tccatcacca acacgcccgc
    15121 catcccgcag gacgttcttg accaatttga gtctatcgtc acgccggatg ccaattcggt
    15181 gttcagctca ctggtgtttt ccgagaaagt ggacgaaaac ttccagcctg tcgagccagc
    15241 ccagaccttc gagaacccgc tgaccgtgat atacggcact tacagctaca atttcatgat
    15301 ggacggcgtg cagtggactg agatgtggta tcgcggaacc accctgctca agctggagac
    15361 cgggcgctgg gaaggcggtt ccggcggata cgccctgacc agcctgagct tgccggccga
    15421 ggaatggctg cccggcgatt atcaggtgca gttctttttg gggaaccagt ggctgatgtc
    15481 tggcagcttc agcgtcagtg gtaacccgcc cacccccacg ctgacaccca gccccacctt
    15541 cacgcgcacg cccaccaata ctcctacctc cacgcgcaca cccgtcccga cccgcacacc
    15601 ggccccgacc agcacgccgc gcccttccgc aaccgcccgc ccgtaggcgc ctttgctcga
    15661 tatcaataga ggctgtccaa aatgaacgga ttttccgatt tggacagccc aaagtgggag
    15721 aaggtgccgg tttaggcttt gttttgttgg cgcaaatctg tatcatccca taaaaatggg
    15781 catggagtgg gaaatcaaat agggccgtcc gactttaagg acagcccctt acaggatttc
    15841 cgatgagaaa aaaaagggag cgcttagatg tagcgccaat ccgtcttttc ttccggcagc
    15901 acgacggcaa tctgcgcggc aattctggca ttattgctca gcagtgccag gttcgtgcgc
    15961 aggctctctc cgccggaaag ttcgttgatt ttcgagagca aatacggcgt aacagcgctt
    16021 ccgcgcacgc cttccgcttc agcctgctgc agcgctgtgg taataagcgc ctccatgtgt
    16081 tcgcgcggca gggcgctttc ggctggtggg gggacgacca ctaacacgct ggtgtggaag
    16141 cccagcttcc aatgcgtgtt ggcaatgttt gccacttcct ggggcgtttc tgcgcgcaaa
    16201 ggggtcttca gaccgctttc gcgggaataa aacgcgggga agtcgtccgt ctgatagccg
    16261 atcaccggca cgccgcttgt ttccaacatc tccagcgtgg ctggcagatt cagaattgct
    16321 ttggcgccgg agcaaaccac cacaagcggt gaacgggaaa gctgccgcag gtcggtggaa
    16381 atatcatacg cagcgccgcg gtgcacgccg ccaatcccgc ccgttgccag cactttgatg
    16441 ccggcgcgct cggcggcata aagcgtgcag gagacggtgg ttccggctga accgccgcgc
    16501 acaatcgcgc cgctcaggtc ccacaggctg cacttgcgcg cctcgcgcgc gccagccagc
    16561 atttcgagct gggccgcgtt caatcccacg cgcagctgac cttcctgaac ggcgattgtg
    16621 gcaggatagg cgcggttatc ccgcacggcc ttttccatgg agcgggcaat gttcaggttt
    16681 tccggccagg ggacgccgtg cgtgatcagg gcggattcca gcgcgacgat ttggcggccg
    16741 ttgcgtttgg cgcgttccac gtcgtctgag atgatgaagt cactttttac agaggttttg
    16801 aagaagggga agatgctttt tttgttttcc atgcttttaa ttgtacatca attcggtgcc
    16861 tggaccgggg aagacccgcg catctgctgc gttttttacg agcccgcccc gggtctttgg
    16921 cggcgaagcg gttacaataa acccaccaaa tactcaggac ttacgatgga aaactctacg
    16981 cttacaccca ccccaaaacg ctggcttgtt tcaccgctga taccccccaa cgtctccagc
    17041 gagctggaag aatttcccag cctgctgcgc caactgcttt ataaccgcgg aatcacagat
    17101 tatcaggcag cgcgtgcctt tctggactgc gcccccgggg cggataccaa tccctttttg
    17161 atgaaggaca tgcaggccgc agtggaaatt ctgcgccagg cggtgcttga aaacgctcca
    17221 attgccatct acggcgatta cgatgtggat ggggtgacct ccagcgcgct gctgtacgag
    17281 ttcctctcgc gcctgggcca aacgccgcgc gtctacatcc ccagccgctt tgacgaaggc
    17341 tacggcttga acctggacgc ggtagagcag ctgcaccgcg agggcattac cctgatgatc
    17401 acggtggact gcggcatccg ctcaatcgcc gaaatcgcgc tggcaaagag cctgggcatg
    17461 cgcgttatcc tcagcgacca tcaccaaccc ggcgcgcagc tgcccccggc ggacgcggta
    17521 atcaacaccc gccaggcggg cgacccttat ccctacaagc agctcgcggg cgtcggtctg
    17581 gcatataaac tggccgacgc gtacctgatc aagtacccgc agaccgggct ggacgcgcag
    17641 gattggttgg acctggtggc gctggggaca gtggcggacc tggcgccgat taacggcgag
    17701 aatcgcgcgc tggtacgggc tgggctggac cagatgcgcg caaccaggcg ccagggtctg
    17761 tactccctcg cgcaggtttc cgggattaac ttggagaacg tcaattcaag caacatcggc
    17821 tttagcttgg gtccgcgcct gaacgcggct ggccggttgg attcggccat ggcggccttt
    17881 gagctgctga cctcgaccga tattttcagc gccggaatgc tggcccagca gttggacgcg
    17941 cagaacgcgc agcgcaagga cctgactttg cgcgtgcagg agcaggccat ccagagcgcc
    18001 ctgcaggcgg accccgacag ccctgtaatt tttgccgcgt cgcccgattt taatgagggc
    18061 gtggtaggtc tggcggcttc gcgcgtagcg gaacagcttt accgcccggc gattatcggc
    18121 tcgcgggggg aaaccaatat tgtagcttcg tgccgctcca tccccgggtt taacatcacc
    18181 aacgcgctgg acgagtgcgc ggatttactt gtgcgctacg gcgggcattc catggcggcc
    18241 ggtctgacgg tcagcctgga aaaagcgccg gagctgctgg cgcgtctgaa cgcaatcgcg
    18301 cgcgaggcgc tgcaggggat ggaactggtg cccgtgctgg ggattgaccg cgaaatccgg
    18361 cttgaacata tgcatcccga gttcgttccg caggtttttg aagcggtgga gaagctggag
    18421 ccgaccggac gcgagaaccc ggaagcgctc ttctgcagcc gggggctgcg ggtggctggc
    18481 gctcgcgcgg ttggggatgg tcagcatctc aagctgacgc tgcaggctgg caggaattcc
    18541 tttgacgcga ttgcctttaa acagggctat tggctgaacc aactgagtga cagcatcgat
    18601 atcgcctaca ccttcgagtt gaacagctac atgggcagga agaccctgca gttgaacgtc
    18661 aaggacattc accccgcgca aggctgactt tcgtcaattc agggaacgat ggcgaaattg
    18721 aggaattttt cttcggcgcg cccgtcttcg tagcgcgtcc gggaagacgc gcccaggcgc
    18781 aaagcaagcc cggattcgca cgcgctggca aggatgccgc gcaggtctga gagcgcgccg
    18841 cccaaagcca gaccttccgg catgccaaaa cggtaggtga agaagcccgg gttgctttcg
    18901 aggtattcca gcacggcgga aagagcggtt gaaaattccg cgttagaaac gctggcggct
    18961 gggtcaatcc aaacgccgac cggaagcttc aggttgcggt gcagcgcgtc caacagcacc
    19021 ggcgagaaaa gcagatgcgc gaacgcctgc ccgcgctccg cggggaggtt ggggagggcg
    19081 ttgggggaag ggctaatagg ctctaaagtg agcgcgtatt ccacgctttc cggcaggtct
    19141 gtccgttcgg atgccgggat gagcgcggtg atgcgctgga gtaccgtgct ggcgtactcc
    19201 tgcagcgcct gcctgcggtc tgcgccctcg gggatttggg cgggcgggat aagctcacct
    19261 acgcgcattt ccagcctggg gcgccggaac ttcagcgcgc tgcccaacgc gccgcgcatg
    19321 ccgccgaagc cgatcggcag aacgggcgca ttcgcgcgct ggctgataag cgccaccccc
    19381 agctgcgctt ccatgtgggc ggggtcccag atgccgcctt cggggaaaat tcccagcacg
    19441 ccgccctgcc gcaagacgtc caccgatttc ttgattgcgt cgcggtccag gttgccgcgg
    19501 ttgatgggga tcagaccgta agcatccacg atacgggcgt agttcgggtc taatggaata
    19561 tcaccggtgc cgaggaattc cacctggcgc ggtgacagcg cggtcatcag caccgcttcc
    19621 aatacgccca catggttgcc agccaggatg agcgggccgc cgcgcgggaa gttttccatg
    19681 ccgcggactt tcaggcgcgt cagcagcgtg aggagcagcc tggcggtgct acgcagaaaa
    19741 gagcgcgcga atttgcgtcg ggggtaaggg aaggagtcgg tgggtttggc gttcatgctt
    19801 gcaggaaaga ttttacctga aaagccgggc gtgattggag ggggaaggtg acagcctgag
    19861 aagacgcgta gaggcattgc tcgcaagcgt ttatgatgat gaagcgctcg tcagcgtgag
    19921 gcacgaagtg gtcttgctgt ttgcatgaag ggagactgcc gcggtcgctg acgctcgctc
    19981 gcagcgacca atccttcgtc atcgcgaggc acgaagcgat ctaccgggtt tcctctgaca
    20041 atccttcgtc atcgcgagac acgaagcgat ctgcctggtt tctcctgtaa aggagagatg
    20101 cgtcacctgc agcccggcct gacaaaggca gccaggacgt tcacctccgg gcctgagtcg
    20161 ctgcgaggca cgaagcgatc taccgggttt cctctgacaa tccttcgtca tcgcgagnnn
    20221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncag cccggcctga caaaggcagc
    20341 caggacgttc acctccgggc ctgagtcgct gctgttggag ggcatgatcc cgaaaaaaag
    20401 acgcgggttg atcgcccgcg tccgatttat caattacctt ccagaaacta cttcatcaca
    20461 acaggcaaac taatgctgta cttgtagaag ggctcccaaa cgtccgtttc accgttgatg
    20521 atgctctggc tgactgcagc tacttcgtcc tgtacgtctt gcgggattga ggctgccgcg
    20581 tcgtgatacg gcgccagacc aacgccctca ttttccagat tattgatcat tgtgccaggt
    20641 gtgaaggtcc cgccaacgac ctcctcaatg gtatggtaga cggcgttatc gacccgcttc
    20701 agcgcgctgg tcagcaggaa ttcgtgcccg tccactgttc caccaccgaa agtgctgtaa
    20761 taggcgtcca cgtccacgcc gattgtccac gcgccagctt gcgcggtttc caggttaact
    20821 ccgattccgg tgggacctgc cacggcaaaa attacatccg cgccgcgcgc catttgatcc
    20881 tgggccatct gctctcccag gggcggattt ccaaagtcat aggagtattc aagcagcacc
    20941 tggacgctgt ggtctgccca ctgcgcgcca tactggtagg gtgtcatgaa ggctgtcacc
    21001 gttggaattg ggaggccgcc gatggcgcca atcaccttgc tcgtgctcat caagccggcc
    21061 agagcgcccg ccaggtatcc gacttgctca gaagcaaagc tgataccgcg caagttcggc
    21121 gggtaggtgt cccaggtcat atccacaatg gcgaagttga cgccaggatt ggcgattgcc
    21181 gcggccatag tcgcgtctcc catcaaccag ccgaccgtga tgcagagcgt gctgcccgcg
    21241 ctcacgcact cgccaatttt cgcggcgtat tcatcggtgc tggcgggttg gtaaattgca
    21301 gggaggatgc cgaagtcggt cgcggcgcgc gctaacccgg cagcggccat ttcgttaaag
    21361 ccgttgtcgt ccacccctgc gacatccggg atgagaccaa ccatgatggt gggcttagag
    21421 gagcttgcga agccggcttg aggagaaagg acgaaggaaa acaagaaaat gagcgtgaca
    21481 cccagtatta gtttacgatg cgtcttgctt tgactgatgc gttggtgaga cataaggtct
    21541 actccttttg tgtagaaata atccgcagag cttcgtgaca cccagcatgt tgccgagcag
    21601 agcgtctgtg cccctgcgct gagggaagaa acaatagtgc gtcttaactt tggtaaagaa
    21661 gaattcgaac attactaaca ttcaactttt ttaccggggt gatgggaaga atgctgtcga
    21721 aagtagaatt tacaggaaag tgcccccaca ggaattcgaa tcctggtttt ggcatacgcc
    21781 ctgcgggcta agggccgcgt cctgggccgc tagatgatgg gggcatgaag gaagtattgc
    21841 tttgttaatc tgcttcggcc gcttttcagg ttttggcata cgccctgcgg gctaagggcc
    21901 gcgtctggag catccggagg aatcccttgt gaggatgaaa aagaaagtgc ccccacagga
    21961 attcgaatcc tggttttggc atacgccctg cgggctaagg gccgcgtcct gggccgctag
    22021 atgatggggg catgaaggaa gtattgcttt gttaatctgc ttcggccgct tttcaggttt
    22081 tggcatacgc cctgcgggct aagggccgcg tctggagcat ccggaggaat cccttgtgag
    22141 gatgaaaaag aaagtgcccc cacaggaatt cgaatcctgg ttttggcctt gagagggccg
    22201 cgtcctgggc cgctagacga tgggggcggt tcaggctata aatgttatca caggctaaaa
    22261 gtcgagtcaa tgattcaagc tgctttccac ggccgcacct gaaacccagg gaaaccgcgg
    22321 cctcgctccg gataccggct ggctttgtcc aatgcttcag gtgcaatccc ggatcaaaac
    22381 gcctataatc aaacaaaaat ggagcgtttc ttatgcaata tcgtcatctt ggaaaagcag
    22441 gtatcaaagt ttcggaattg tccatcggtt cctgggtcac ctacggcaag caggtggatg
    22501 tgcgggctgc ccgcgacatc ctgcaaaccg cctgggaagc cggggtcaac ttttttgaca
    22561 acgccgaagc ttacgcaggc ggacaatccg aaataattat ggggaaagcc ctgaaggaat
    22621 tggctttccc gcgcgagcag tatctcattt cgaccaaaat cttctggggc ggcaaaggac
    22681 ccaacgacac cggtctgaac tacaagcaca tcatcgaggg cgtgaataac gcgctaaaac
    22741 ggcttggact ggaatatgtg gacttcgtgt ttgcgcaccg ccccgacccc gaaaccccta
    22801 tggaagaagt ggtatggggc ttcaatcaaa ttattcagca aggtaaagcg ttttattggg
    22861 gcacctcgga atggtctgcc gcccaaatta tgcaagccta tcaggtggcc aaggaatatc
    22921 atctgcgccc gccttccatg gaacaggtgc agtacaatct gctgcaccgc cggcgctttg
    22981 aaaaggagta cgccgtgctc tacaaggagc tgggctacgg caccacggtt tggagcccgc
    23041 tcgccggtgg cttgctcact ggcaagtata acgatggcaa aatcccggag cacagccgcg
    23101 ccacgctttc cggttgggag tgggtcaaag atgacgtgct cgatccagag aagctcaaag
    23161 taacgcgtaa attagctgag atcgctgcgg atttgggcgt cagcatggcg cagatgtcgt
    23221 tagcttggct gctcaagaac ccgaatgttt ccaccgtgat taccggcgcc agccgcgtgg
    23281 aacaggtgcg cgagaacatg aaggctgccg atgtggtgcc gctgctgacc gatgaggtca
    23341 tggcgcggat tgaggccgcg ttggaaggct accgggagga gtaacctctt tgaataccag
    23401 acgaggacgc ggtctgcccg cgtcctcgtc tttcgttgcg aaacaacaaa attctggaga
    23461 agccttcttt agttttcaac agcatgagga agcttctggc cctccattcc cctctccccc
    23521 cagcgccctg gcctgcgcct cgcg
//
LOCUS       DAOQAD010000021        22532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_2328_length_22532_cov_5.387374, whole genome shotgun sequence.
ACCESSION   DAOQAD010000021 DAOQAD010000000
VERSION     DAOQAD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 22532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22532
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2328_length_22532_cov_5.387374"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4750)
                     /locus_tag="PKL60_03125"
     CDS             complement(<1..4750)
                     /locus_tag="PKL60_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNW95159.1"
                     /translation="MLSRNEPDLKTARRRRKIDWHALIIVFLLLSILCGGAYFRTIGV
                     DWDAGRHLHPDERFLSMVLNSMTPVKSVGEYFNTDASTLNPGNVGYDFFVYGTLPLFI
                     IRYVGEWVGQVGYDPITIVGRQLSALADILTILLVFLIARRLFNSKTGLLAAALYAFA
                     VLPIQQSHFMTVDTFTNTFGMLTVYIAVGILTRPGPQLSGEKPRPRRLGLRYWFPYAA
                     FGAALGMAAASKINAVSLALLLPAVEFVHFFRLKPEEREVYGKISLKMIVLAALVSLL
                     SFRIFQPYAFSGTSFFTSGLNREWLDGLKSLAQQSTGNVDFPPALQWARRSVGFSLYN
                     LVVWGLGIPFGVFSVLSLAAMGWAGIKKRRFDLLPVWGWTVFYLLWQLTAWVKAMRYL
                     LLIYPLLAVMAAWGITRLLQTNRELRIRRFKLSAKTLRTLGTIFAVIAVVGTAAYAFA
                     FTRIYTRPHTRVAATKWIYNTLPGPVNLVMTGEAGEKLLQAPYRSGDALYGDQAHPFV
                     FTSWTDGGLESFSIPTAQYLSAQGAFNRFTVTIERAGDLGAPLTLPVLSSGTPSNLAG
                     SSASGLEFQFEPPVPLEKGAGYVIRLVQSDPNSQVMLSGVPKVSVRNSAGALVPQSLD
                     KIVETISPAHSYNMDVSVLTSGNIDAVEIPFLLDLSQNFAEKTLKITLSPSAEADNQP
                     VSGTVSGTFIDMGGGKGTSTRIALDKPLRISTPQVVSLSLEMTEGKGALALSSVAPVH
                     ESSWDDALPVSEPGYVPYSDNGGIFRGDLNLELYWPDNADKRERFIDLLQQGDYLFIS
                     SNRQWGTTTRVPERYPLTSFYYRELLGCPENLDLVSCYNTAEPGMYQGSLGFDLVKTV
                     TSYPNLGSWQFNDQFAEEAFSVYDHPKVLIFKKNAGFDAVHIREIFESVDLSYVVNLT
                     PRQADKYDTSIARANNLMLSSSALAVQREGGTWSTLFDRLSLVNLSQSAAVALFYLFS
                     FGLGLFVYPILRLALPGLADKGYAFSRLAGFLLIAWFAFILGSSGFAVSRTLIGAICA
                     LIALAGVLAAVLGWRKLWPEVKQNWKHYLVIEVLALAAFLFFLWVRYNNPDLWHPYKG
                     GEKPMDFSYFNAVIKSTTFPAYDPWFAGGYINYYYYGFVILGMPVKLLGIIPSVAYNI
                     ILPLWYSLLVIGAYSVGWNITRAMIAARDGENAAELRRPFGRPFAAGVCTALLLAFVG
                     NLGTVKLLSQTLQVMGAGGTAVDGAGVFSRLSWLFKGFGQLLKGTPMPLYPGDWYWFP
                     SRVIPGDPITEFPYFTFLYADLHAHLIAMPIAVFAVGWGLSVLLSKCKWGGNRVEKWL
                     GFALSFFIGALVIGALKPTNTWDYYTFLLLNLFVLGYCGWKYLKPAKILCREWVSKLL
                     PAAGMIALLTVLSILLYAPFNRLFHPGYSQIGFWTGDRTPLSSYFTHWGLLLFIVVFW
                     YAWETYQWLAVTPLSALKKLEPHQTMLKIMGVLFGGVLMMLLLMKVSAAIIVLPLCLW
                     SLALILRSGQSDAKKLMFFMIATALLETLVVELVYLVGDIGRMNVVFKLYMQAWLLMA
                     LAAGSGLGLLWKDQHKWTLRTQLLFQLPLIL"
     gene            complement(4755..6869)
                     /locus_tag="PKL60_03130"
     CDS             complement(4755..6869)
                     /locus_tag="PKL60_03130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNW95160.1"
                     /translation="MENRPASDETEITILEYLKSLLARGAGQGQGSVGAAAAEEDQEP
                     STKSAAAAVRDAVNFPWFSTGAALLALIAQLFAVNRMALFSLLFFAAAAACLLLGLRA
                     KEWKLPLNSGSPEPWQFFTGKTWLLFPGLALGAAAFFLFRSQRFTLTATLCWAAAVTA
                     VLLAFWNKAARDKTARAPLNLRIQKLLKEERAWFFMLLLVSAVVAWFSFAHLKAVPPE
                     LVSGEVDHIYSVRDVLSGNAVLLHSRNLVSEPLQYLWGALFSFAAGGQTGFVELKAAY
                     ALANLAALYFIYRLAADLFDRWVGLTAALLLGISFWQIIQTRALLGSGLVLPLACAAL
                     YFLFSGLQKHQANDLALSALFAGLGLLTNKIFLIFPVVALVVGLIWLVTNHREGSTKA
                     FFSAAAQALLLGAVVSVPLLRAISLEPQAYFAPILSRVSDYETALSGSPVRIFLTNLW
                     NALGIVNWSNRSSWVDGLTLRPGMDLLSAAFFGVGVCAAAFTLRKTKDWRVLALLALY
                     PLLLLPSAMSLAFPLENPSLSRALTALVPACILSSIGLRIFLGALFAHSPAGKGSYKL
                     VLSALLLLFAIGLNDRAAFSEYPTTFRQNAWNNTEMANVIRQTELDTGYPQNVWVIGY
                     PYWVDARAVALEAQRPDQVLGITADQLEQTANVLGPKMFLIHPLDSATVEKLQQLYPT
                     GTIAMVQSSLPDKNFLVFLVKQ"
     gene            complement(6936..7748)
                     /locus_tag="PKL60_03135"
     CDS             complement(6936..7748)
                     /locus_tag="PKL60_03135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNW95161.1"
                     /translation="MSFLRKWSYYGWSLLEMAFGFRDWGKAARLFLRKTGQEPVVLRL
                     RRQDARILVRSRMDAWSVKETFLDRFYTRYGTEIQADWTLVDIGAAIGEFTIFAAQQA
                     ARGKVVAFEPNPQSFSLLQENLKLNSIRNVQAVLAGVWSKPGMMTLDAVNGEPLQAQT
                     RFSEAELGSGQGVPVLTLDQLLEKYAPQGVDLLKMDCEGAEYPILFSANAKTLGAVRR
                     IVMEYHDLDESQNHHELAGFLRQAGFEVHLSANPVHKEIGYLYAWFPPEFAR"
     gene            complement(7745..8554)
                     /locus_tag="PKL60_03140"
     CDS             complement(7745..8554)
                     /locus_tag="PKL60_03140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW95162.1"
                     /translation="MKTRLDPALLDEIAWKHLSTLPYFRGMLRAVEHSLYARFELRAP
                     ILDIGAGDGNFAAALGSDANSLGADPWYEPIREAAALGGYRMLVQAEGAALPVGDAKF
                     PCAISNSVLEHIPDVQPVLDEVSRALTPDGVFYFTVPNQRFKSDLWGCAVLSKLGMRG
                     LAERYSLFFNRIARHHNLDKPEVWVARLHKAGFAQVEHWNYFQVKAMRMLERGHAAGL
                     PNLLWKKLFGKWVLFPKRRNPFLRYKRLRPLLDNPLCEDGTCTFFAARKGS"
     gene            complement(8551..9699)
                     /locus_tag="PKL60_03145"
     CDS             complement(8551..9699)
                     /locus_tag="PKL60_03145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNW95163.1"
                     /translation="MKVLISSTYFTPYSSGLSVYAHRLAQGLVSLGHEVVVLTSQHQA
                     ELPLEESLQGFRVVRQPVRWRLSKGVLMPGLMKTAREWISWADAVNLHLPQFEAISLA
                     REAKRQAKPIIVTYHCDLDLQGGWFNRLAGLVTQSLSARVLDMADLIVQNSLDYARNS
                     RWLQPHLPKIREVHPPVEMIKADPADIQRFKQKRGIAEGERVLGLAGRAAAEKGYEYL
                     IEAMPQILQRFPTARILHAGTWKGVIGEEQYQARIEEMAKPLGERWHSVGYLSDEEFR
                     AFFGACDLLVFSSLNSTESFGIVQIEAMLQGTPVVASDLPGVRQPVLKTGMGRIVPPR
                     DSSALADAICSVLTEGPHAYAAPAKYLESFGINQAALRYHALLETLLA"
     gene            complement(9696..10718)
                     /locus_tag="PKL60_03150"
     CDS             complement(9696..10718)
                     /locus_tag="PKL60_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNW95164.1"
                     /translation="MPQQDEKRRSADFLRWLLPLLVTAAAVYFVGREIDFSQVGEAFA
                     RISLSNLLLVLGLFALSLVLRVVCWYFLLEGGFTFKEAFYGVNAGYLLNNVLPLKLGE
                     FGRAALLAGRGKAGFLSVFASIVTERVLDFFLAAFFFLVSLPLVVTSQSVKTLAIVIL
                     ALTVVGMLGAALAAKNKQRISDSLSARWAEKPRLLGLLPRAMNFLEGFRILLRPGRFF
                     AALLLLAASWASSMAEVWVLTRALIPQSQWWWGIFVTSGSAFANALPTAPASMGVYEA
                     ANVAAFSVLGVDQSTALVIALILHAVQFLVTSLLGMLGIYLLGDSLGKLSKKALSWKQ
                     QQEKAG"
     gene            complement(10718..12511)
                     /gene="lepA"
                     /locus_tag="PKL60_03155"
     CDS             complement(10718..12511)
                     /gene="lepA"
                     /locus_tag="PKL60_03155"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNW95165.1"
                     /translation="MEKIRNFCIIAHVDHGKSTLADRMLQLTGTISDRDMTEQVLDTM
                     DLEREKGVTIKASAVRMIYHSRDAQDYEFNLIDTPGHVDFNYEVSRALYACEGAVLVV
                     DATQGIEAQTLANLYLALDANLVIIPVINKIDLPSAQVEAVKKDLTNLLGVTGDEIIP
                     ISAKTGLNVELVLEAIAQKIPAPTRDIDAPLRALIFDSHYDSYKGVIAYVRVFNGHLT
                     SGDKVMMMANGIRVQPVEIGIFSPNLFPIDKLEAGDVGYVATGLKTVSECRVGDTITN
                     AVHPAEHALPGYRQAKPMVYAGVYPTEGEDYPNLKEALEKLQLNDASLVYEPETSEAL
                     NFGFRCGFLGLFHMEIIQERLEREYNLDVVFTAPSVEYEVQLTNGNTIIVDSPADLPD
                     EGLITEIREPWMELNVFSPTEYYGVVMDLVRRKRGVYISEEYPSANRVQLNFEIPLSE
                     IIVDFFDLLKSGTRGYASMDYQFLEYRAGNLVKLEFLLNEEKVDALTAIVHKDDAYHK
                     GQALVSKLKEIIPQQLFVIPIQAYSQGRVISRANVKAMRKDVLAKCYGGDITRKKKLL
                     EKQKKGKKRMKMVGSVEIPQEAFMAILKLEN"
     gene            complement(12538..13332)
                     /locus_tag="PKL60_03160"
     CDS             complement(12538..13332)
                     /locus_tag="PKL60_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNW95166.1"
                     /translation="MKRIRLDQHLVNLGLAETRTRAQRAIDEGLIKVNGSIAVKASQL
                     VQPDCSVALLAQPEFVSRAGAKLKAALDSFKVSPLAKVCADVGTSTGGFTDCLLQRGA
                     SKVFAIDVGQDELHTSLRNHPLIVVMDHTNARHLSRLPEIPELVTIDVSFISLDFILP
                     AVRGWFEGKPGDVIALVKPQFEAGREEVKKNRGVIRDRAIHARVLRHVLTQAQALGYV
                     PAGLITSPINGSDGNKEFLLWLRLGCGGDCLDTDEAIARVIPSEIS"
     gene            13850..14719
                     /locus_tag="PKL60_03165"
     CDS             13850..14719
                     /locus_tag="PKL60_03165"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNW95167.1"
                     /translation="MNAYLETLRPKQWIKNLVIFAGLVFDRQLLDLEPLLRVLAAVVL
                     FCLVSGLTYTINDILDVEADRLHPQKKNRPIASGRLSLRSARIFAVILVLLSFPAAFA
                     LSVKFGMICLVYTALMLSYSKWLKKFILIDVMVIALGFVLRVAAGVIVIKVNYFSPWL
                     FVLTTLLALYLGFGKRLSEIKLLEDQAGEHRKVLYGYTVPLLNNLLVVILSGILITYC
                     LYTFSSHPDGTNTLMMFTIPFVLYGIFRYMVILQNSSLASSPEEVLLNDRPLQITVLL
                     WALSVVVILYFFF"
     gene            14729..15688
                     /gene="mvk"
                     /locus_tag="PKL60_03170"
     CDS             14729..15688
                     /gene="mvk"
                     /locus_tag="PKL60_03170"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNW95168.1"
                     /translation="MPAIAASACAKLILLGEHAVVYGEPALAIPFSGLRARAAVEAWI
                     GAPSGRIHVFARELDLDDEWSALPENNLIRSALSLVLTELEIERLPTCKLQLNCEIPI
                     SGGLGASAALAVAVMRAFSAFLGHPLNTERLNALAYKSETLMHGTPSGIDNTVVVYEQ
                     PIIFARDRGFAVIRPAGDLHFLVADSGVKKATAQTVSQLAQDYTQNPGGIGPILRDIG
                     QLSMEGVYALNQGDLPALGEALNANQELLSRLNLSCPELDALVFAARKAGALGAKLTG
                     GGKGGHMLALVQPEALDQVRAALLQAGAPHVYQTLLKQEPRHA"
     gene            15681..16487
                     /locus_tag="PKL60_03175"
     CDS             15681..16487
                     /locus_tag="PKL60_03175"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HNW95169.1"
                     /translation="MLDTNLNPDLIFIKLGGSLITDKDRAETADFAMLADLLAEISRI
                     RGKHPNLKLLLGHGSGSFGHHAARLAGTRNGVFTPADWQGYQKVWASARRLNQIVVEA
                     CLQAQLPVLSFPPSASVVTDQHEIQSWELAPLRSALSNGLIPLVYGDVVIDRALGGTI
                     LSTEDLFFHLALVLKPAKILLAGEEEAVFADFPLNQQPLPFLSKDADNADFLQGSASL
                     DVTGGMLSKVQLMQSLCRRLPGMKAVIFSGRKPRNLADCLDGMIIGTTIG"
     gene            16585..17739
                     /locus_tag="PKL60_03180"
     CDS             16585..17739
                     /locus_tag="PKL60_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNW95170.1"
                     /translation="MIYTRQQLEETENQTLAPYAVHSKDSKGRVYPEEEADFRTRFQR
                     DRERILHTTAFRRLEYKTQVFINHEGDYYRTRLTHSLEVAQIGRSVARSLGANEDLVE
                     TICLAHDLGHPPFGHSGEAALARLMRDYGGFNHNQQSLRIVTTLENRYPDFPGLNLSW
                     EVVEGMVKHETDYDIADAKDYNPELRGHLEAQIANVADELAYTSHDLDDGLRSGMITP
                     TQLDGIAIWEIINESIGRRKTESLDELSRHQIIRRLINFEVTDLIQSIDRYLRKSNVK
                     SVLEIQKLPYNVVGFSEDMYRRNRELKDFLFANLYRHFRVVRMSAKAEKIITELFGIY
                     QKMPATLPAGVQDLVPERGLERTICDYIAGMTDRFAIDEYKRLFEADVLP"
     gene            17759..18226
                     /gene="greA"
                     /locus_tag="PKL60_03185"
     CDS             17759..18226
                     /gene="greA"
                     /locus_tag="PKL60_03185"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNW95171.1"
                     /translation="MKNPLIITKTGKAKLEAELAELKGPRRQDISRRLKSAIEMGDLS
                     ENADYIAAKEDQGFLEGRILEIESILANAEVVDEEKVRSDKVQIGNKVTVREGNEDEE
                     VFELVGISEVNTMLGKISYESPIGAALLEKRPGETVRVQTPGGTLEFTIIKIE"
     gene            complement(18290..19417)
                     /locus_tag="PKL60_03190"
     CDS             complement(18290..19417)
                     /locus_tag="PKL60_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95172.1"
                     /translation="MENFIDFLKQKLSVPRNMAVAAGLLGILLGLLIGWVVLPVQWTD
                     TNAETMRADLQVDYLRMVITNYNLTFDAAKAKQLYQELGKSAGATLTLVQADPQFLNA
                     DQINRFITAVQAEPAEALPPDENPDQTGADTAGSPLKVGLILLGLLVVGAAGVYMFYL
                     RPRTKPAGTPLQQARPAAVPQAVPRASGATAQTQGVQTNGKSAANHGSAAQPEVPAYE
                     SPAYVRPNGKGTQTLEKPIAQFMPTYMFGDDLYDESFTFDAPNGEFLGECGVSISDII
                     GVGEPKKISAFEVWLFDKNDIQTVTKVMMSEHVFHDPVLRQRLEMKGEPILAEAGRLF
                     TLQTASLRMEARIVDLTYGDLPLPPQSYFQRATIELAVYRI"
     gene            complement(19478..21880)
                     /gene="topA"
                     /locus_tag="PKL60_03195"
     CDS             complement(19478..21880)
                     /gene="topA"
                     /locus_tag="PKL60_03195"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNW95173.1"
                     /translation="MEAYCLKCKEKREMLAPTASFTVNGRAITKGTCAVCGGVMSKLG
                     MSDAHAGLEKPVVQSAPKKETRKPAKKVGAEKSASKKNRAGGKAAVAGSRAQGKNLVI
                     VESPAKSRTIGKYLGDKYLVVASVGHIRDLLKSQLSVDVENNFTPKYRVPNEKKALVR
                     EITNLADQSDKVYLATDPDREGEAIAWHLMDTMGISPERTERVVFHEITKDAISEAFA
                     HPREIAMDLVNAQQARRILDRLVGYGVSPILWAKVRGGLTAGRVQSVALRLIVERERE
                     IEAFVPVEYWLISAEFTPDGSTKHYHAKLVRINQAEPILPDEATTLGVVEDLRQASYQ
                     LDSLKTTQKNVYPKAPHITSTLQQDASQRLGFPSRKTMLIAQQLYEGIDIGNGGETGL
                     ITYMRTDSVHVAPQAVQEARSLILRKYGEVFLPEKPPIYKTRAASAQEAHEAIRPTSV
                     LREPASIKDHLTADQFKLYTLIWQRFLASQMSPALLEIITAEISGKSAANTYLFRASH
                     TRTLFKGYQVLFQEAPGEDEQDEEEDGELPIAVLHEGQTQHLEDLEYSQHFTQPPPRF
                     TEASLIKVLEENKIGRPSTYSPIISTILARGYVTTEKKSLIPTETGFLVNDLVVEFFP
                     SIVDIRFTSEMEDELDKIADGKQGWVDVIREFYQPFEASLKFAQENMPEKRIEPEKLG
                     RSCPQCGSELVLRNGRFGKFVSCSAYPNCKYTENHLERIGVVCPTCKEGDVVRKRTRK
                     GRVFYGCSRYPDCDFTSWDEPLKQRCPQCGGVLVAKNKHFARCLACSEVFTLESLKSE
                     PD"
     gene            complement(21987..>22532)
                     /locus_tag="PKL60_03200"
     CDS             complement(21987..>22532)
                     /locus_tag="PKL60_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNW95174.1"
                     /translation="RALAESIITHGALISDYPPGTQPDGVNFPPRNRIISGLSRGTVV
                     IEAGEKSGALITARFSIDQGREVFAAPGSVLSPMSRGTNNLLAAGAVPLTSPTVILKH
                     LNIAQTRPNQEQAKPALDLEEARILRALGNDTLHIDDLCSKLGTGVEKLTAQLTMMEL
                     KGLVVRARGMEYCASKTWILD"
BASE COUNT         5103 a         6489 c         6138 g         4802 t
ORIGIN      
        1 gcaaaatcag cggaagttgg aataagagct gcgtgcgcag cgtccatttg tgctggtctt
       61 tccacagcaa ccccaggccg ctgccagctg ccagcgccat caacagccag gcttgcatat
      121 acagcttgaa aaccacgttc atccgcccga tgtcccccac caggtagacc agctccacca
      181 ccagtgtctc cagcagcgcg gtcgcaatca tgaagaacat cagcttcttc gcgtcgcttt
      241 gccctgaccg taagattaag gccagcgacc acaagcacag cggaagcacg atgattgctg
      301 cgctcacttt catcaacaaa agcatcatca acacgccgcc gaagagtacg cccataattt
      361 ttagcatggt ctgatgcggt tcaagcttct taagcgcaga gagcggcgtt accgccagcc
      421 actggtatgt ttcccaggcg taccaaaaaa cgacaataaa gagcagcagc ccccaatgcg
      481 taaagtaaga gcttagcggc gtccggtcgc cagtccagaa tccaatctgg ctatagccgg
      541 ggtgaaacaa ccggttgaac ggcgcgtaca gcagtatgct gagcacggtc aggagtgcaa
      601 tcatgccggc agcaggcagc agcttgctca cccattcccg gcacaggatt tttgcaggct
      661 tcaggtactt ccagccgcag tagccaagca caaaaaggtt cagcagcaga aaagtgtagt
      721 aatcccaggt gttcgtcggt ttcaatgcgc cgataacaag cgcgccgatg aaaaagctga
      781 gggcgaaacc aagccatttt tccacgcggt tcccgcccca tttgcatttt gagagcagca
      841 ccgaaagccc ccagcctacc gcgaagaccg cgatgggcat agcaatcagg tgggcatgca
      901 aatccgcgta gaggaaggtg aaatacggga attccgtgat ggggtccccc ggaatcaccc
      961 ggctggggaa ccaataccaa tcccccgggt agagcggcat tggggtgcct ttgagcagct
     1021 gcccaaagcc tttgaagagc caggaaagcc tgctgaacac gccggctccg tcaacggccg
     1081 tgccgcctgc gcccatcact tgcagcgttt gcgaaaggag cttgaccgtg cccagattgc
     1141 cgacaaaagc cagcagcaag gctgtgcaca cgcctgccgc gaagggcctg ccaaaaggtc
     1201 tgcggagttc cgcggcattt tcaccgtccc gcgcggcaat catcgcgcgg gtgatgttcc
     1261 agcccacgct atacgcgcca atgacaagca gcgagtacca cagcggcagg atgatgttgt
     1321 aggccacgga tggaatgatg cccagcagtt tgaccggcat ccccaggata acaaagccgt
     1381 agtagtagta atttatatac ccgccggcaa accagggatc ataagccggg aaagtcgtac
     1441 ttttaatcac cgcgttaaag taggaaaagt ccatcggctt ttcgccgccc ttgtacggat
     1501 gccacaggtc cgggttgttg tagcgcaccc acaggaagaa aaggaaggca gccagagcga
     1561 gcacttcgat gacaaggtaa tgcttccagt tttgcttcac ttccggccat agcttgcgcc
     1621 agccaagaac cgcagcaagt acgccagcca gggcaatcag cgcacaaata gcgccgataa
     1681 gcgttctgct tacggcaaaa ccgcttgagc ccaggataaa ggcaaaccag gcaatcagca
     1741 aaaagcccgc taacctgcta aaggcataac ccttatctgc taatccgggc agcgccaacc
     1801 ttaggatggg atacacgaag aggcccaacc caaagctgaa gaggtaaaaa agcgcgaccg
     1861 ccgcgctctg gctgaggtta actagcgaca gcctatcgaa caaggtgctc caggttcccc
     1921 cctcgcgttg tacggccaaa gcgctgctgc tgagcatcag gttgttggct ctggcgatgc
     1981 tggtgtcgta cttgtcggct tggcggggtg tcagattgac cacatagctg aggtccacac
     2041 tctcgaagat ttcccggatg tgcacggcgt caaagccggc gtttttcttg aaaatcagca
     2101 ccttagggtg gtcgtatacg gaaaacgctt cttccgcgaa ctggtcgttg aactgccagc
     2161 ttcccaggtt cggataggat gtgacggttt tgaccaggtc aaaacccagg ctgccctggt
     2221 acatgcccgg ttccgcggta ttgtaacagc ttaccaggtc caggttttcc gggcagccca
     2281 gcagctcgcg gtaatagaaa cttgtcagcg ggtagcgttc cggaacgcgg gtggttgtgc
     2341 cccactgccg gttggagctg atgaagaggt aatccccctg ctgcagcaag tcgatgaagc
     2401 gttcgcgctt gtcggcgttg tccggccagt aaagctccag attcaggtcg ccgcggaaaa
     2461 tcccgccgtt atcgctgtac ggcacgtaac ccggctcaga aacaggcagc gcgtcatccc
     2521 acgaggactc gtgcacagga gcgaccgagg aaagtgccag cgcgcctttc ccttctgtca
     2581 tctcaagcga gagactaacc acctgcgggg tagaaatcct gagtggctta tccaatgcga
     2641 tgcgcgtgct ggtgcccttc ccgccgccca tatcgataaa agtaccggag actgtgccgc
     2701 taaccggctg attatccgcc tccgcgctgg gactcagtgt gatcttaagc gttttttcgg
     2761 caaagttctg gctgaggtcc aggagaaagg gtatttccac agcgtcgata tttccggagg
     2821 tcaagacgct gacatccatg ttgtaggagt gtgcagggct aatcgtttcg acgattttat
     2881 cgagggactg gggcacgagg gcgcccgcgc tgtttcgcac gctcactttt gggacgccgc
     2941 tgagcatcac ctgcgaattc gggtcagact ggacaagacg gatgacatac ccggcgcctt
     3001 tttccaacgg aacaggcggt tcaaattgga attccagccc gctggcgctg ctgcccgcga
     3061 ggttcgaagg agtcccggag gaaagcacag gcagcgtgag gggggcgccc aaatcgcccg
     3121 cgcgctcgat cgtgacagta aagcggttga aagcgccttg cgcactcagg tattgcgcgg
     3181 tgggaattga aaagctttcc agcccgccgt ctgtccaact tgtgaacaca aaggggtgcg
     3241 cctggtctcc gtagagcgca tcgccgctgc ggtaaggagc ctgcagcagc ttttcgccgg
     3301 cttcaccggt catcaccaga ttcacgggtc caggcagtgt attgtagatc cacttggtcg
     3361 cggcaacacg cgtatgcggg cgcgtgtaaa tccgcgtgaa ggcgaaggca taggcagccg
     3421 tccccacgac tgcaatgacg gcaaagatgg tacccagggt tcgcagggtt ttggcggaaa
     3481 gtttgaaccg cctgatgcgc agctcccggt ttgtttgcag caggcgggtg atgccccagg
     3541 ctgccatgac agccagcaaa gggtaaatca gcagcaggta acgcatggct tttacccagg
     3601 ctgtgagctg ccagaggagg tagaacacag tccaacccca aactggaaga agatcgaatc
     3661 tccgtttctt tatgcctgcc caacccatag ctgccaggct gagcactgag aaaacgccaa
     3721 aaggtatgcc cagcccccag accacgaggt tgtagagcga aaacccgaca gacctgcgcg
     3781 cccattgcag tgccggcggg aaatccacat tgccagtgct ttgctgcgcc aggctcttaa
     3841 gcccgtccaa ccattcgcgg ttaagtcccg acgtgaaaaa gctggtaccc gaaaaggcat
     3901 acggctggaa gatgcgaaag ctcagcagcg agaccagcgc agccagaaca atcattttta
     3961 agctgatttt gccgtagact tcgcgctcct ccggtttcag gcggaaaaag tgcacgaatt
     4021 ccacggctgg aagcagcagc gcgagcgaaa ccgcgtttat ttttgacgca gccgccatcc
     4081 caagcgcggc gccaaaggcc gcgtatggga accaataccg caagcctaac ctgcgtggcc
     4141 gcggcttttc accagaaagc tgtggacccg gcctggtcag gatacctacg gcgatgtaga
     4201 cagttaacat gccaaaggtg ttcgtgaagg tatcaaccgt catgaaatgc gactgctgga
     4261 tgggcagcac agcaaatgcg tacaacgcag ccgccagcaa gccggttttg gagttgaaca
     4321 ggcgccgcgc aatgaggaac accaacagga tagttaagat atccgccagc gcggaaagct
     4381 gcctgcccac gatggttatc gggtcatagc ccacctgccc cacccactcg ccaacatacc
     4441 ggatgataaa aagcggcagc gtgccgtaaa cgaaaaagtc gtaaccgaca ttacccggat
     4501 tcagcgtgga ggcatctgtg ttgaagtact cacccacgct cttcaccggc gtcatgctgt
     4561 tcaataccat ggagaggaag cgttcatccg ggtgcagatg gcggccggca tcccaatcaa
     4621 cccctattgt gcggaaatac gcgccaccgc acagaatcga aagcagcaga aaaacgatga
     4681 tgagagcatg ccaatcaatt tttctgcgtc gtcttgccgt tttgaggtcg ggttcgtttc
     4741 tgctgagcat aagtctattg ttttaccagg aaaacgagaa aatttttatc aggcagagag
     4801 gattgcacca tggcgattgt ccctgtggga tacaactgct gcagcttctc cactgttgcg
     4861 ctgtccagcg ggtgaatcag aaacattttg ggaccaagca cattcgctgt ctgctccaat
     4921 tggtcagccg taatcccaag aacctggtcc gggcgctgcg cttccagcgc tacagcccgc
     4981 gcgtccaccc aatagggata gccaattacc caaacattct gcgggtaacc agtatcgagc
     5041 tccgtctgcc ggatgacgtt tgccatctct gtattgttcc aggcattctg ccgaaacgtc
     5101 gttgggtatt cgctaaaggc agcccgatca ttcaatccga tggcaaaaag aagaagcaga
     5161 gctgacaaaa caagcttgta actgcctttg ccggcaggcg aatgggcaaa cagcgcgccg
     5221 aggaaaatcc gcagcccaat agacgacaga atacacgccg gaaccagggc agtcagcgcg
     5281 cggctgagcg aaggattttc cagcggaaac gcgagcgaca tcgcgctggg gaggagcaga
     5341 agcgggtaga gcgccagcag cgccaggact cgccagtctt ttgttttccg caaagtgaag
     5401 gctgccgcgc aaacaccaac cccaaaaaac gccgcgctca acaggtccat ccccgggcgc
     5461 agcgtcaaac cgtccaccca actactgcgg ttggaccagt tgacgattcc cagggcgttc
     5521 cacagattcg tgaggaaaat ccgaacaggg cttccggaaa gggctgtctc gtaatccgac
     5581 acgcgcgaaa ggatcggcgc aaagtaggcc tgcggttcca gggatatcgc gcgcagaagc
     5641 ggcacgctga ccaccgcgcc cagcagcagc gcctgggcag ccgcgctgaa gaaggccttc
     5701 gtgctgcctt cccggtgatt ggtcaccagc cagattaagc caacaaccag cgcgaccacc
     5761 gggaaaatca ggaatatttt atttgtaagc agtcccaagc cagcaaaaag cgccgagaga
     5821 gctaaatcgt tggcctggtg tttttgcaag ccgctgaata ggaagtacag cgcagcgcaa
     5881 gccaacggca gcaccagccc ggaaccgagc agcgcacggg tttggatgat ttgccagaaa
     5941 gatatcccca gcaacaaagc cgccgtcaaa cctacccagc ggtcaaaaag gtccgcagcc
     6001 aagcgataga tgaaatacag cgccgcgagg ttcgccagcg cgtaagcggc cttaagctcc
     6061 acaaaacctg tctgcccgcc cgcggcgaag ctgaacagcg cgccccacag gtattggagc
     6121 ggttcgctca ccaggttgcg cgaatgcaac agcacggcat tccctgagag cacgtcccgc
     6181 acactgtaga tatggtccac ctcaccgctg accaattccg gcggcactgc tttgagatgg
     6241 gcgaacgaaa accatgcgac cacggcgctg accagtagca gcataaagaa ccaggcgcgc
     6301 tcttctttca gcagtttctg aattcgcagg tttaaggggg cgcgcgctgt tttgtccctg
     6361 gcggctttgt tccagaacgc aagcaagaca gcagttactg ccgccgccca gcagagtgtg
     6421 gcagtaagcg tgaagcgctg cgagcgaaac agaaagaagg ctgcagcgcc aagcgcaagg
     6481 ccgggaaaaa gcagccatgt tttgccagtg aaaaactgcc agggttctgg cgaacctgag
     6541 ttgagcggaa gcttccactc ctttgcccgc aaacccagca acagacaggc agccgctgcg
     6601 gcaaaaaaaa gcaggctgaa gagcgccatg cggttgacgg caaataactg ggcaataaga
     6661 gcgagcaaag cagcgccagt ggaaaaccag ggaaaattta ccgcgtccct caccgctgct
     6721 gcagcgcttt tggtggaggg ctcctggtcc tcttccgcag ccgccgcgcc gacactaccc
     6781 tgcccctgcc cggcaccacg cgccagcagt gatttcaggt actcgaggat tgtaatttcg
     6841 gtctcgtcag acgcaggtcg gttttccatc ttgcccaatc ctattgcgaa gaaacaggat
     6901 gcgttttgtt tgtcgtattt tagcccggaa agcgcctatc tggcaaattc aggcggaaac
     6961 caggcgtaca ggtagccaat ttccttatgc acagggttgg cggataaatg cacctcaaag
     7021 ccggcctgcc gcaaaaatcc cgccaattca tgatggtttt gactttcgtc caggtcatgg
     7081 tattccataa caattctcct gacggcgcca agggtcttcg cgttggcgga gaacaggatg
     7141 gggtactccg ccccttcgca gtccattttc agcaggtcga cgccttgcgg cgcgtatttt
     7201 tccagcagct ggtcgagcgt aagcaccgga acgccctgac cgctcccgag ctccgcttcc
     7261 gagaagcggg tctgagcctg caagggttcg ccattgacgg cgtccagcgt catcataccg
     7321 ggcttgctcc atacgccggc gagtacagcc tgcacattcc ggatggaatt caacttcaga
     7381 ttttcttgca gcagggagaa tgactgcggg ttcggttcga acgcgaccac cttccctcgt
     7441 gccgcctgct gagcggcaaa gatggtgaac tccccaatag cggcgccgat atccacaagc
     7501 gtccaatccg cctgaatttc ggtgccgtac cgtgtgtaga agcggtctaa aaaagtctct
     7561 ttcacggacc aggcatccat ccggctgcgc accagtatgc gggcatcttg ccggcgcaga
     7621 cgcagaacca cgggctcctg tcctgtttta cgcaggaaca agcgcgccgc tttgccccaa
     7681 tcgcgaaaac cgaaagccat ttcgagcagc gaccagccgt aatacgacca tttccgcagg
     7741 aagctcatga gcctttcctc gcggcgaaaa aggtgcaggt gccgtcctca cacaagggat
     7801 tgtccagcag cgggcgcagg cgcttgtagc gcaagaaagg atttctacgc ttggggaata
     7861 acacccattt gccgaagagc ttcttccaca gcaaattggg cagacctgcg gcatgcccgc
     7921 gttcgagcat tctcatagcc ttgacctgga aataattcca atgctccacc tgcgcgaagc
     7981 ccgctttatg gagccgcgcg acccatacct cgggtttatc caggttatga tgccgggcaa
     8041 tgcggttgaa aaacaacgaa tatctttccg ccaagccccg catcccgagc ttgctcagca
     8101 ctgcgcagcc ccacaaatcg ctcttaaagc gctggtttgg cactgtgaag tagaaaactc
     8161 catccggcgt tagcgcgcgg ctgacttcgt ccagcaccgg ctgcacatcg gggatgtgct
     8221 caagcacgga gttacttatg gcgcatggga atttcgcgtc acctaccggc agcgcggcgc
     8281 cttctgcctg caccagcata cggtagcccc ccagagccgc ggcttcccga atcggttcat
     8341 accaagggtc cgcgcccagg ctgttcgcgt cgcttccgag cgccgccgcg aagttgccgt
     8401 cccccgcgcc aatatccagg atgggagccc gcagctcgaa tcgggcgtac agactgtgct
     8461 ccacagcgcg cagcatacct cgaaagtacg gcagggtgga aagatgcttc caggcgattt
     8521 cgtccagcaa ggcgggatct aaacgcgttt tcatgccagc agcgtctcga gcagcgcatg
     8581 atagcggagc gccgcctggt ttatcccaaa agattcgagg tactttgccg gggcggcata
     8641 cgcgtgcgga ccttccgtca gcacggaaca aatcgcatcc gccagcgcag aggaatcgcg
     8701 cggcgggacg atgcgcccca ttccggtttt caggaccggc tggcgcactc ccggtaaatc
     8761 cgacgcgact acgggcgtgc cctgcagcat ggcttcaatt tgcacgatgc caaacgattc
     8821 cgtgctgttc aggctggaaa acaccagtaa atcgcaagcg ccaaaaaacg cccggaattc
     8881 ttcatcgctc aggtatccga ccgaatgcca gcgctcgccc agcggcttcg ccatttcctc
     8941 aatgcgtgcc tggtattgtt cttcccctat gacgcctttc caggttccgg catgcaggat
     9001 gcgcgcggtt gggaaacgct gcaggatttg cggcatggct tcgatcaggt attcatagcc
     9061 tttttccgcc gcggctctgc cggccagccc gaggacgcgt tcgccttctg cgatgccgcg
     9121 cttctgctta aagcgctgaa tgtccgccgg gtcggccttg atcatctcaa ccggcgggtg
     9181 cacttcccga attttcggca ggtgcggctg cagccagcgc gaattccggg cgtaatccag
     9241 cgagttttgc acgatgaggt cagccatatc caacacccgc gcgctcaagg actgcgtgac
     9301 caagccggcc agacggttaa accagcctcc ttggagatcc aggtcgcagt ggtaggtcac
     9361 aattatgggt ttcgcctgcc ttttggcttc acgcgccaag gatatcgctt cgaactgcgg
     9421 aaggtgcagg ttgaccgcgt ccgcccagct tatccattcc cgcgcggttt tcatcagccc
     9481 cggcatcaac acgcctttgg aaaggcgcca gcgtactggt tgacgcacca ccctaaagcc
     9541 ttgtaaggac tcttccaggg gcagctctgc ttggtgctgg gaggtgagta ccaccacttc
     9601 gtgcccgagt gagaccaaac cctgagccag ccggtgcgca taaaccgaca aacccgagga
     9661 atacggcgtg aagtaggtgg aggatatcag cactttcatc cggctttttc ctgctgctgt
     9721 ttccagctta gcgctttctt gctgagtttg ccaaggctgt cgcccagcag gtaaattccg
     9781 agcataccca acaaacttgt aaccaggaac tgcaccgcgt gcaaaatcag cgcgataacc
     9841 agcgccgtcg attggtccac gcccaaaacg ctgaaggcgg cgacattggc ggcttcgtag
     9901 acacccatgc tggccggcgc ggtggggagc gcgtttgcga acgcgctgcc cgaagtcacg
     9961 aatattcccc accaccattg gctttgcgga atgagcgcgc gcgtcaggac ccaaacttct
    10021 gccatcgagc ttgcccaact ggcagccagc agcaggagcg ccgcgaagaa acgccccggc
    10081 cgcagcaaga ttcggaagcc ttccagaaag ttcatcgcgc ggggaagcag cccaagcaat
    10141 cgcggttttt ccgcccagcg cgcgctgagg gaatcagaaa tgcgctgttt gtttttcgca
    10201 gccagcgcgg cgcccagcat cccaaccacg gtcagcgcca ggataacaat tgccagcgtc
    10261 ttcacagatt ggctggtaac gaccaggggc aggctgacca gaaagaaaaa cgctgccaga
    10321 aagaaatcga gcacccgttc cgtcacgatg ctggcaaata ccgataaaaa gccggccttg
    10381 cctcggcctg ccaacagcgc ggcgcgcccg aattccccca gcttgagcgg gagcacgttg
    10441 ttcagcaggt agccggcgtt caccccataa aacgcttcct tgaaagtgaa cccgccttcc
    10501 agcagaaaat accagcacac gacgcgcaag accaggctca aagcaaacag ccccagcacc
    10561 agcagcaggt tagacaggga tatccgcgcg aaagcctcgc ctacctggga aaaatcaatc
    10621 tcccttccaa caaaataaac tgccgctgcg gtgacaagaa gcggcagcag ccatcgtaag
    10681 aaatccgccg accggcgctt ttcatcctgc tgcggcatca gttctccagt ttaaggatag
    10741 ccataaacgc ttcctgtggg atctctacgc tcccgaccat tttcatgcgc tttttgcctt
    10801 ttttctgctt ttccagcagc tttttcttgc gggtgatatc ccccccgtag catttcgcca
    10861 gaacgtcttt gcgcattgcc ttgacgttcg cgcgcgagat gaccctgccc tgcgagtagg
    10921 cttgaatcgg gatgacgaac agctgctgcg ggatgatttc tttcagcttg cttaccaacg
    10981 cctgcccttt atggtaggcg tcgtctttat gtacaatcgc ggtcagcgcg tccacttttt
    11041 cctcgttcag caggaattcc agcttgacga gattgcctgc ccggtactcg aggaattgat
    11101 aatccatgga agcatagccg cgcgtgccgg acttgagcaa gtcaaagaag tccacgataa
    11161 tttccgaaag cggaatttca aagttcaact ggacgcgatt ggccgacgga tattcttcgg
    11221 agatgtatac gccccgtttg cgccgcacca ggtccatcac cacaccgtag tactcggtcg
    11281 gggaaaaaac gttcagttcc atccagggtt cgcggatttc ggtaatcagg ccttcatccg
    11341 gcaggtccgc cggggagtct acgataatgg tgttcccgtt agtaagctgg acttcgtatt
    11401 caacggaagg cgcggtaaag accacatcca ggttgtattc ccgctccagc cgctcctgga
    11461 taatttccat gtggaacaat cccaaaaagc cgcagcggaa accgaagttc agcgcttcgc
    11521 tggtttccgg ttcatatacc agcgaggcat cattcagctg cagtttttcc agcgcttctt
    11581 taagattggg ataatcctcc ccttcagtcg ggtacacgcc ggcatagacc attggtttgg
    11641 cctggcggta gcctggcagc gcgtgttccg ccgggtgaac agcgttggta atcgtatcgc
    11701 caaccctgca ttccgacacg gttttgagcc cggtagcgac ataaccaacg tcacccgctt
    11761 cgagtttatc aatcggaaag aggttcggcg agaaaatgcc aatttcgaca ggctgaacgc
    11821 ggattccatt cgccatcatc ataactttat ccccgctggt cagatggccg ttgaaaacgc
    11881 gcacataagc aatgacgcct ttatatgaat catagtggga gtcaaaaatc agcgcgcgca
    11941 gcggagcgtc gatatcccgc gtaggcgccg ggattttttg ggcaatcgct tccaatacca
    12001 gctcgacgtt caggccggtt ttcgcgctga tgggaataat ttcgtccccg gtcactccca
    12061 ggaggttagt gaggtccttt ttgaccgcct ccacttgcgc gctgggcagg tcaatcttgt
    12121 tgattaccgg aatgatgacc aggttggcgt ccagggcaag gtagaggttg gcgagcgttt
    12181 gggcttcgat gccctgggtg gcatccacca ccaacaccgc gccttcgcaa gcatacagcg
    12241 cgcggctgac ttcatagtta aaatccacgt gccccggggt atcaatcaag ttgaattcgt
    12301 aatcctgggc gtcgcggctg tggtaaatca tcctgaccgc ggaggctttg atggtaacgc
    12361 ctttttcccg ctccaaatcc atggtatcga gcacttgctc agtcatatcg cggtccgaaa
    12421 tagtgccggt aagctgcagc atccgatccg ccagagtgga tttgccgtga tctacatgcg
    12481 cgatgataca aaaattgcgg attttttcca taggcgcatt gagtataatc gatttcttca
    12541 gctgatttct gaggggatta cccgcgcaat cgcctcgtcc gtatcgaggc agtctccgcc
    12601 gcagcccaat cgcagccaca acaaaaattc tttattcccg tccgaaccgt taatcggcga
    12661 agttatcagt cccgccggca cgtagcccag cgcctgcgct tgcgtcagga catgccgcag
    12721 tacgcgggcg tggatggctc tatcccggat gaccccgcgg tttttcttca cttcttccct
    12781 gccggcctca aactgcggct ttaccagcgc aatcacatcg cctggtttcc cctcaaacca
    12841 gccccggacc gcaggcaata tgaagtccag tgagatgaaa gatacatcga tggtgaccag
    12901 ttcagggatt tccggcaggc ggctcaggtg gcgggcgttg gtatggtcca tcaccacgat
    12961 gagaggatgg ttgcgcaggc tggtgtgcag ttcgtcctgc ccgacgtcaa tcgcgaaaac
    13021 tttgcttgcg ccgcgctgca gcaggcagtc cgtaaaccct cccgtggacg tgccgacgtc
    13081 cgcgcatact ttggccagag ggctgacctt gaagctgtcc agggcggctt tcagttttgc
    13141 ccccgcccgg gaaacaaatt cgggctgcgc cagcagcgcc accgaacaat ccggctggac
    13201 aagctgcgag gctttcaccg cgattgagcc gttcaccttg ataagacctt cgtcgatggc
    13261 tctttgggct ctggtgcggg tttccgccag ccccaggttt accaggtgtt ggtccagtct
    13321 aatcctcttc atgcttctat tgtatccgct tatgccagcg gtgcgaatcg ataagcccgg
    13381 gcagcagaac aaagcaagac tgtcttgaaa tagcaaaata gaaatgctct cgtgatgatt
    13441 gggagctttg aaaacttcat tcgatacccc cgtgttttta caaaggaata aaaataccgt
    13501 gacacaaggt agcagcagct tttcctctcg tcccccactt cccagcgaac gaaaattaac
    13561 caatacttct caggaacgct cgctggaacg gggagttgat cttggacagc gggagccgcc
    13621 gtccatcaac cgtttccctt ctccagcggc gcggccaagg caaaatagat ccgcttgccg
    13681 ccggggaaga gtcagggttt gggaaagcat aaaacatgat agaaaagaag aaacaaagcg
    13741 gttcgttacg aggaaaaacc cgtccggatg actcatccac gaattaggac atttctattt
    13801 agccctgaca tcgaaaagcc ttttgttgct ttgtgataag ataattcccg tgaacgctta
    13861 tctggaaacc ttgcgtccaa agcaatggat caagaacctg gtaattttcg cgggtttggt
    13921 cttcgaccgg cagctgctgg atttggagcc gctgctgcgt gtacttgccg cggtggtcct
    13981 gttttgcctg gtttccgggc tcacctacac gatcaacgac attctggacg tggaagcaga
    14041 ccgcttgcat cctcagaaga agaatcgccc gattgccagc ggcagattga gcctgcgcag
    14101 cgcccgcatt tttgcggtaa ttctggttct gctcagcttc ccggcggcgt tcgcgctctc
    14161 agtgaaattt ggaatgatct gcctggttta taccgcgtta atgctttcat actccaagtg
    14221 gctgaagaag ttcatcctga ttgacgtgat ggtcatagcc ctcggttttg tgctgcgggt
    14281 ggcagccggt gtgattgtaa tcaaggtcaa ctacttttcc ccgtggttgt tcgtcctcac
    14341 aaccctgctc gcgttatatc ttggatttgg caagcgcctg tcagaaatca agctgctgga
    14401 agatcaagct ggcgaacacc gcaaggtttt gtacggatac acagtaccgc tgctcaataa
    14461 cctgcttgtg gtcattctga gcggcatcct catcacctac tgcctgtata ccttttcatc
    14521 ccacccggat ggaacaaaca ccttgatgat gttcacaatc ccattcgttt tgtacgggat
    14581 tttccggtac atggtcatcc tgcagaacag cagcctggct tcctcgccag aggaagtact
    14641 cctgaatgac cgcccgctgc aaatcaccgt tctgttatgg gctttgtccg tcgtggttat
    14701 tctctacttc tttttctaag gagaacaaat gccggcaatt gctgcaagcg cttgcgcgaa
    14761 attgatttta ttaggcgagc acgctgtagt gtatggcgaa cccgctctgg caattccctt
    14821 ttccggatta cgggcccgcg ccgcagtgga agcctggatt ggcgctccct cgggcaggat
    14881 tcacgtcttt gcgcgcgagc tggacctgga tgatgaatgg tccgcgctgc cagaaaacaa
    14941 cctcatccgc agcgcgctca gcctggtgct gaccgaactg gaaatcgaac gcctgcccac
    15001 ctgtaaattg cagctcaact gcgagattcc catttcaggc ggcttaggcg ccagcgccgc
    15061 gctggctgtg gctgtgatgc gcgctttttc tgcctttctt ggtcatccgc tcaatacgga
    15121 acggttgaac gcgctggcct acaagtctga aaccttgatg cacggcacgc cctccggcat
    15181 tgacaacacc gtggtcgttt acgaacagcc gattattttt gccagggacc gcggttttgc
    15241 ggtcatccgt cctgccgggg atttgcattt cctggtagca gacagcggcg tgaagaaagc
    15301 caccgctcag acggtcagtc agttagcgca agactacacg caaaacccgg ggggtatcgg
    15361 gccaattctt cgcgacatag gtcagctgag catggaaggc gtgtacgcgc tgaaccaggg
    15421 ggatttgccc gccctgggcg aggctctgaa cgccaaccag gaactgcttt cgcgcttgaa
    15481 cctctcctgc ccggagctgg atgcgctggt tttcgccgcg cggaaagctg gcgccttagg
    15541 agccaaatta accggcggcg gaaaaggcgg gcacatgctt gccctggtgc agcctgaagc
    15601 gctggaccag gtgcgggctg ccttgctgca agccggcgcg ccgcacgtct accaaaccct
    15661 gctcaagcag gagccccggc atgcttgata ccaacctcaa tcctgatttg attttcatca
    15721 aattgggcgg gtcgctcatc acggataaag accgcgccga gaccgctgac tttgcgatgt
    15781 tggcggactt gctggcggaa atctcccgga tacgcggaaa gcacccaaac ctgaagctgc
    15841 tgttagggca cggctcgggt tcattcgggc accacgccgc ccgcctggca ggcacgcgca
    15901 acggtgtctt tacccccgcg gactggcagg gttatcagaa ggtgtgggct agtgcgcgcc
    15961 gactgaacca aatcgtggtt gaagcctgcc tgcaagcgca gctgccggta ctttctttcc
    16021 caccctccgc gtctgtagtg acagaccagc acgaaattca gtcttgggag cttgccccgc
    16081 tccggagcgc actatccaac gggctgattc ccctggtata cggcgacgtc gtgatcgacc
    16141 gggcgcttgg tggaacaatt ctttccaccg aagacttatt ctttcacctc gcgcttgtcc
    16201 tcaaacccgc caaaatcctg ctggcaggcg aagaagaggc ggttttcgcg gactttccgc
    16261 ttaatcagca gccgctacct tttctcagca aagacgctga taacgccgac ttcctgcagg
    16321 gttccgccag cctggacgtt accggcggga tgctgagcaa agtgcagttg atgcaaagcc
    16381 tgtgcaggcg gctgcccggc atgaaagcag taattttctc cggaaggaag ccccgcaatc
    16441 tggcagattg cctggatgga atgatcatcg gaaccacaat tggctgatga gcgctttgta
    16501 caccgactcc acgatgctat ctccgcttgc ccgctataat ggtggcaatg ctatgcgctt
    16561 gagcgcggaa acggataatg acctatgatt tacacccggc agcagctgga agaaaccgaa
    16621 aatcagactc tggcgcccta cgccgtgcac agtaaagact cgaaaggccg cgtctacccg
    16681 gaagaagaag cggacttccg cactcgtttc cagcgggacc gggaacgcat tctgcacacc
    16741 accgccttcc gccgcctgga atacaagacc caggttttta tcaaccacga aggcgattat
    16801 taccgcacgc gcctgacgca ttccctggag gtcgcccaaa ttggacgctc agttgcccgg
    16861 tcgctcggcg ccaacgagga cctggtggaa acgatctgcc tggcgcatga cctggggcac
    16921 ccgcctttcg ggcactcagg ggaggcagcc cttgcccgtc tgatgcggga ttacggcggt
    16981 tttaaccaca accagcagtc cctgcgcatc gttaccacgc tggaaaaccg ctacccggat
    17041 tttcccggct taaatctgag ttgggaagtc gtggaaggca tggtcaaaca cgaaaccgac
    17101 tacgacatcg cggacgcaaa agattacaac cccgagctgc gcgggcacct ggaagcgcaa
    17161 atcgccaacg tagcggacga actggcctat acctcccacg acctggatga cggcttgcgc
    17221 tccgggatga tcacgcccac ccagttggac ggtatcgcca tttgggagat tatcaacgag
    17281 agtatcggac gccgcaaaac ggagagcctg gacgaactct cccgccatca gattatccgc
    17341 cgcctgatta acttcgaagt taccgacctg atccaatcca ttgaccgcta tctgcgcaag
    17401 agcaatgtca aatccgtcct ggaaatccag aaactgccgt ataatgtggt gggattcagc
    17461 gaggatatgt accgccgcaa ccgggaactg aaagatttcc tgttcgcgaa cctttaccgg
    17521 catttccgag tggtgcgcat gtccgcgaaa gcggaaaaga ttatcacgga acttttcggg
    17581 atctatcaaa aaatgcctgc cacgctgccg gctggcgttc aggacctggt accggaacgc
    17641 gggctggaaa ggactatctg cgattacatc gccgggatga ccgatcgctt tgccatcgat
    17701 gaatataaac gtctgtttga agcggacgta ttaccttaga aagcagaacc aaacttttat
    17761 gaagaatcca ctcatcatca ccaaaacagg gaaagccaaa ctggaggcag aactggcaga
    17821 gttgaaagga ccccgccgac aggatatttc acgccggctg aagagcgcaa tcgagatggg
    17881 cgacctttcc gaaaacgcgg attatatcgc cgcgaaggaa gaccagggct ttctggaagg
    17941 ccgcatcctg gaaattgaga gcatcctcgc gaacgccgag gttgtcgatg aagaaaaagt
    18001 tcgttccgat aaagtccaaa tcgggaataa ggtgaccgtc cgtgaaggga acgaggacga
    18061 agaagttttt gagctggtgg gtatcagtga agtgaacacg atgctcggga aaatttctta
    18121 cgagtcgcca attggcgccg ccctgctgga gaaacgaccg ggggagaccg tgagagtaca
    18181 aactccaggc ggaacactgg aattcaccat tatcaaaatc gaataaagaa aaaacaacaa
    18241 aaaagctgag ggcgaactgc acctcagctt tttttgtttt cctgggcggc taaatacgat
    18301 aaaccgccag ttcgatagtc gcgcgttgga agtagctctg cggaggcaag ggcaaatcgc
    18361 cgtaggtaag atcaacaatg cgggcttcca tgcgcaggct ggcggtctgc agcgtaaaca
    18421 gcctgccggc ttcagccaga atgggttcgc ccttcatttc cagacgctgg cggagcaccg
    18481 ggtcatggaa gacatgctcg ctcatcatca ccttggtgac ggtctgaata tcattcttgt
    18541 caaacagcca aacctcgaac gccgatatct ttttaggctc cccaacgcca ataatgtccg
    18601 aaatgctgac gccgcactcc cccaggaatt cgccgttggg ggcatcaaac gtgaaggact
    18661 cgtcatacag gtcatcacca aacatatagg tgggcataaa ctgcgcgata ggtttttcca
    18721 gggtctgggt gcctttccca ttcggacgaa cataagccgg actctcatag gcaggtactt
    18781 ctggttgcgc cgcgcttcca tgattcgccg cagactttcc gttggtttgc accccctggg
    18841 tttgcgctgt cgcgccggag gcccggggaa ccgcttgcgg tacggcagcc ggcctggctt
    18901 gctgcagcgg tgttccagcc ggtttggttc tggggcgcaa atagaacata tacacgccgg
    18961 cagctcctac caccaacaaa cccagcagaa ttaagcctac cttgagcggg cttcctgcgg
    19021 tgtcggcacc ggtctggtcc gggttttcat ccggcggcag cgcttcggca ggttccgcct
    19081 gaacggcagt aataaagcgg ttgatttggt cagcgttcag gaactgcggg tcagcctgga
    19141 ccagggtgag ggttgcgccg gcgcttttgc ccaattcctg gtagagctgt ttggctttgg
    19201 cagcatcaaa ggttaggttg tagttggtaa tcaccatgcg caggtaatcc acctgcaaat
    19261 cggcgcgcat ggtctccgcg ttggtatcgg tccactgcac aggcaaaacg acccatccaa
    19321 ttaacaagcc aagcaggata cccaacagtc ccgcggctac agccatgttt ctcgggacgc
    19381 tgagcttctg cttcaaaaaa tcgatgaaat tctccataat cctgcacctc acagtcaaag
    19441 aatgctctga ttggtgagtt gcaggattta taccagatta atccggttcg gatttgaggg
    19501 attcgagcgt aaagacctct gagcaagcca ggcagcgggc aaagtgtttg ttcttcgcga
    19561 ccaaaactcc cccgcactgc ggacatcttt gttttagcgg ttcatcccag gaagtaaagt
    19621 cacaatctgg ataacgggaa cagccgtaaa aaacgcgccc tttacgcgtg cgtttgcgca
    19681 ccacatcgcc ttccttacag gtcgggcaaa caacgcctat cctttccagg tggttctcag
    19741 tgtacttgca gtttggatac gcgctgcagc tgacgaattt gccaaatctg ccattgcgca
    19801 gaaccaattc gcttccgcac tgtgggcagg atcggcccag tttttccggc tctatgcgtt
    19861 tttcgggcat attttcctgg gcgaatttca ggctggcttc aaaaggctgg taaaactcgc
    19921 ggatgacatc cacccaaccc tgttttccgt cagcgatttt atccagctca tcctccattt
    19981 cggaggtaaa acgaatatcc acaatcgatg ggaagaactc gaccaccagg tcgttcacca
    20041 ggaaacctgt ttcagtcggg atgaggctct tcttttcagt cgtaacatac ccgcgcgcca
    20101 atatcgtgga gatgataggc gaataagtgg acggtctgcc aattttgttc tcttccagca
    20161 ctttgatgag tgaagcttca gtaaagcgcg gcggcggctg ggtaaaatgc tgggaatact
    20221 ccaggtcctc gagatgctgt gtctgccctt catgcagaac tgcgattggg agttctccat
    20281 cctcttcttc gtcctgttcg tcttccccgg gcgcttcctg aaacaaaact tgatagcctt
    20341 tgaaaagcgt cctggtgtga gaggcgcgga ataggtatgt atttgcagcg cttttccctg
    20401 aaatttcagc ggttattatt tccagcaagg ccgggctcat ttgtgaagcg agaaatcgct
    20461 gccagatgag agtatagagt ttgaattggt ccgctgtcag gtggtccttg atggaggcag
    20521 gttcgcgcag aaccgaggtc ggtcggatgg cttcatgcgc ttcctgcgcg gaggctgctc
    20581 tggttttgta gattggcggc ttctcgggca ggaatacttc gccgtatttc ctgaggatta
    20641 agctgcgcgc ttcctgcaca gcctgcggcg cgacgtggac agaatccgtg cgcatgtaag
    20701 tgattaagcc tgtttcgccg ccgttgccga tgtcgatacc ttcgtataac tgctgggcaa
    20761 tcagcatcgt cttcctggac ggaaatccaa gcctttggga ggcatcctgt tggagcgtac
    20821 tggtgatgtg cggggctttg ggatacacat ttttctgcgt ggtcttcagc gaatcgagtt
    20881 gatagctggc ctggcgcagg tcttcaacca ctccaagcgt ggtcgcttca tccggaagga
    20941 tgggctcagc ctgattaatt cgcaccaatt tggcatggta atgtttcgtg ctgccatcgg
    21001 gcgtgaactc agccgaaatc agccagtatt ccaccggcac aaacgcttca atttcccgct
    21061 cgcgttcgac aatcaagcgc agcgcgacgg attggacccg cccggctgta aggcctccgc
    21121 ggactttcgc ccacaaaatc gggctgacgc cgtaacccac cagcctgtcc aggatacggc
    21181 gggcttgctg cgcgtttacc aaatccatgg caatttcacg cgggtgcgcg aaggcttcgc
    21241 ttatggcgtc cttggtgatt tcatggaata ccacccgttc cgttctttcg ggggaaatac
    21301 ccatcgtatc catcagatgc caggcaatcg cttcgccttc ccggtcgggg tcagtcgcca
    21361 ggtaaacttt atcgctttgg tcagccagat ttgtaatctc ccgcaccagc gctttctttt
    21421 catttgggac gcggtatttg ggcgtgaaat tattttccac atccacgctc agctgggact
    21481 tcaagagatc acgaatgtga ccgacggaag ccacaacaag atacttatcc ccaagatatt
    21541 ttccaattgt tcgggatttc gccggcgatt cgacaatgac aaggttcttg ccctgcgctc
    21601 tgctgccagc aacagcagct tttccccctg ccctgttctt ctttgaggcg cttttctccg
    21661 cgccgacctt tttcgcgggt ttccgggttt ccttttttgg cgcgctctga acgaccggct
    21721 tttccaagcc agcgtgggca tccgacatgc caagtttgga cattacgccg ccgcaaacgg
    21781 cgcaggtccc cttggtaatc gctctcccat tcaccgtaaa actggcagta ggcgccagca
    21841 tttcccgttt ttctttacat ttaagacaat aagcttccaa aagaatgctc ctgaatattc
    21901 atacctgaaa attgcccacc tataatacca tattcagttt tgtgtcaagc cggcgcatac
    21961 cctttcttat tgtctgtgga gagactttaa tccagtatcc aggtcttgct ggcacaatat
    22021 tccatccccc gtgcccgtac gaccaagccc ttaagctcca tcattgtgag ctgggcggtc
    22081 agtttttcca ctcccgtacc taacttgctg cataggtcgt caatatgcag cgtatcgttc
    22141 cccagcgcgc gcaggatgcg cgcttcttcc aggtccaggg caggtttggc ctgttcctgg
    22201 ttcgggcggg tttgggcaat gttcaggtgt ttcaggatga cagtcgggct ggtaagcggc
    22261 acggctccag ctgccagcaa attgttggta ccccggctca tcggcgaaag cacagacccg
    22321 ggcgcggcaa acacttccct gccctggtca atgctgaacc tggcagtaat cagcgcgccg
    22381 ctcttctcgc ccgcttcaat caccaccgtc ccgcgtgaaa gccccgagat aatccggttt
    22441 ctcggcggga agttcacccc atccggctgt gtaccgggcg ggtaatcgct gattaacgcc
    22501 ccgtgcgtga tgatgctttc cgccagagcg cg
//
LOCUS       DAOQAD010000022        21730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_2451_length_21730_cov_5.307958, whole genome shotgun sequence.
ACCESSION   DAOQAD010000022 DAOQAD010000000
VERSION     DAOQAD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 21730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21730
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2451_length_21730_cov_5.307958"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PKL60_03205"
     CDS             <1..571
                     /locus_tag="PKL60_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95175.1"
                     /translation="LAPTAIPTPLPNPTSAPTLPPALTTLPVDESLPALLGHPEDLPS
                     GYKPGSLGSIDEDRFAWITNRGSASHLLSIEKGDNGVEGYVAVFVYEDTLDPALSYMD
                     DLNEVTRLIQEYGSSYTFEFDTIDDVGEKDMAYVMSASSSSEANIAVGFVRCNLYVRI
                     SFMNSKDRAMIISYAQKLDDRIIALGCQY"
     gene            705..1616
                     /gene="fmt"
                     /locus_tag="PKL60_03210"
     CDS             705..1616
                     /gene="fmt"
                     /locus_tag="PKL60_03210"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNW95176.1"
                     /translation="MNARVVFMGSPDFALPTLEELHNNTQVVGVVTQPDRPAGRGRQP
                     MPCPVKQLADHLRIPVFQPLSLRKPGALEQLEAWQPDLIVVAAFGQILPKSVLALPPK
                     GCLNVHASLLPRWRGASPIQAAILAGDERSGVTIMLMEEGLDTGPILTQRTVPIRPGT
                     TGAELSQQLAFLGAATLTEILPSYLSGMLAPLMQADSLATYAPMLKKSDGLLDFHQPA
                     LTLTRKVHAYFPWPGTYFVLNGKQIKVLDAHPHDTFESEIEGHYIVNGLPAVGTSEGL
                     LVLDRLQPEGKRGMPGSDFLNGQPGWL"
     gene            complement(2225..4792)
                     /gene="mutS"
                     /locus_tag="PKL60_03215"
     CDS             complement(2225..4792)
                     /gene="mutS"
                     /locus_tag="PKL60_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNW95177.1"
                     /translation="MAQDITPLRQQYLDIKKQYPDAILFFRLGDFYETFDDDARKASE
                     ALDIVLTSRPVARGVRVPMAGIPFHAADNYLGRLIEKGYRVAIAEQVGGQPDKGLFTR
                     EVVRVVTPGTVVEPGLLSAGRNNYLAAVCLQENRAGIAYLDITTGEFTGTQLDLEVNL
                     NALRAELIRINPAETLRPEGQELSESFPGRLTPVPAWYFEPGRAEEQLKSVFGVASLD
                     GFGLKNLPLTVRAAGALLKYLQETHKDALLTLKSFGTYSLGDFMVLDAETRRNLELTE
                     TLRDAKSEGSLLWVLDQTKTPMGKRMLRQWVNKPLLNLAAITARLDRVESFYRDGVRR
                     NELTHFLADFHDMERLVNRVCTNSIRPPELLSLREDLRLLPKLRELLPTEPALAALLA
                     ELHEHPDVLSLLDEAIADDPPATLANIGVIKPGFSAELDAIVSSSQHAREWINNLEGE
                     ERKRTGIKTLRVGFNKVFGYYIEISRGSADNAPADYLRKQTLVNAERFITPELKEYES
                     LVLNAETQIHEVELRVFRSVCQRVASSAALLLESARALAALDCVLSLAAAAALNNYVR
                     PTVRAEKGLKIIGGRHPVVERLRTGERFIANDAVFEEGEIVRVITGPNMSGKSTFLRQ
                     VVLIVLLAQVGSFVPAESAEIGLVDRIFTRIGAQDAIHAGQSTFMVEMIETANILNNA
                     TPRSLLILDEIGRGTSTYDGLSIAWAIIEYLHSHPKLKPFTLFATHYHELTELAEMLP
                     GVRNYNVAVTESEGKVVFLHKIVPGGADRSYGIHVAQLAGLPGPVIQRANELLKQLEA
                     TSGKTIPAQVPDSSQLRLFPENNPLMDAFKKVDLNSLSPIQALNLLYEWRRNFFPEE"
     gene            complement(4801..7530)
                     /gene="alaS"
                     /locus_tag="PKL60_03220"
     CDS             complement(4801..7530)
                     /gene="alaS"
                     /locus_tag="PKL60_03220"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNW95178.1"
                     /translation="MPLTGNQIRQSFIDFFTERGHTHVPSASLVPGGDSTLLFTNAGM
                     VQFKDVFLGTDSRPYTRAVDSQKCMRVSGKHNDLDQVGRDNTHHTFFEMLGNWSFGDY
                     YKKEALSWSWELLTEVWGLDKSRLWATTFKDEYGQIPDDEEAAAVWLSQPGFDPTHLV
                     FSGRKDNFWEMAETGPCGPCSEIHYDFGPEACTQKDDPQHVCDVNASCGRWTEVWNNV
                     FIQYNRLSPTELAPLPKKHVDTGMGFERIVSIIQGVRGNYATDLLSPLLDEVQLLTGQ
                     TDAQRAENLTPYRVIADHVRASAFLIADGVIPGNLGRNYICRMIIRRAARFARKLGLT
                     EPFMAKVAARVISIYQDAYPELKRSESLILDTLTREEQRFAETLDAGFAELSRLIAGM
                     RSRGESVLNGKAAFDLYATLGFPLEITRDILGEEGLGVDESGFFESMEAHRAASGAGK
                     AFGDMGGRDAENYQRVLQELIKNHALGEAGCAYDPYDRYVVPSTVLALFQNAQPVRQV
                     QAGDEVEVLLGDTSFYLESGGQVSDTGKITSDPAGRWEIEVSGLRKPAAGVIVHVGRV
                     VFGLPTVGENAVARVDKPRRQAIMRNHTATHLLHAELRKTLSDQVRQAGSLVAPDRLR
                     FDFNYPSAISHEQLLRIEKGVNQAVLGEYDLNITVKPLEKAINEGAMALFGEKYGQEV
                     RTIKIGDEETISYELCGGTHVHNTSEIGLFLITSESSVAAGIRRIEAVTGLAAYAFAR
                     ERLNLLQQSAQMVGASPAELPVRLTALQDELEKAEKRIEQLSAQIAQEAFNRQLDQVR
                     VISGVPVLTAQVPGAGAEMLRGLADQFRQRYPSGVVALASVLDDKPLIIAAVTPDLIK
                     RGLKAGDLVKRAAAVVGGGGGGKPDLAQAGGKDPGKISEALDQVPAYVAETLR"
     gene            complement(8088..8696)
                     /gene="recR"
                     /locus_tag="PKL60_03225"
     CDS             complement(8088..8696)
                     /gene="recR"
                     /locus_tag="PKL60_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNW95179.1"
                     /translation="MRTLPDPVQNLIDAFARLPGIGPKTASRLAFYLLKAPDESSLQL
                     AEALEQLKSQTQHCRTCFNVTSAGQDQCEICASDNRDESILCVVEEPLDVLAIERTGA
                     FNGKYHVLLGVLSPIEGIGPDQIKIFPLLERLKGGTVREVIIATNPSMEGDATALYLQ
                     QRILPLGIRVSRLARGLPVGGDIEYADQNTLLRALAGRQSME"
     gene            complement(8704..9048)
                     /locus_tag="PKL60_03230"
     CDS             complement(8704..9048)
                     /locus_tag="PKL60_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNW95180.1"
                     /translation="MAKGYNAGPSARPGGNMMAQIAKLQEQMAAAQAEISETQLTETA
                     GGGAVTVTMTGDQKCVAVSIQEDVLKEADVEMLQDLLLIAINKALDGTRALSEEKMAP
                     FANLLGGMGMGR"
     gene            complement(9050..10729)
                     /gene="dnaX"
                     /locus_tag="PKL60_03235"
     CDS             complement(9050..10729)
                     /gene="dnaX"
                     /locus_tag="PKL60_03235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNW95181.1"
                     /translation="MTQALYRTWRPASWEEVAGQEHIVQTLRNAVISGRVNQAYLFAG
                     PRGTGKTTSARLLAKAVNCLAEDPAARPCNTCENCKAVNEGRFLDLIEIDAASNTSVE
                     DIRELRDTINFAPSQGKFKVYIIDEVHMLSTSAFNALLKTLEEPPSHVIFILATTEIH
                     KIPATVLSRCQRHEFRRIPLNQIVGKLRQICEQEGIGIDEDALSLIARQSTGSMRDAI
                     SLVDQLASLPGRVTLAAAQDILGTATSQTVMDLVQAVQDKDSARGLELIHGALDGGAD
                     PRQFARQVVDYLRSLLVVKMNGGKDLELLPEVRSVVNKQAGAFGAGDLISGIDEFSRA
                     AAELKANWYPGLSLELAFARSVSATAAPVLAAEPAALAPVRNSAQGPGQKEPASETPA
                     RKSTPAPEQSKTAGDSQKDRKTEDKVGERKPETSASAESAPRESGELNLENLLQDWDK
                     IKRDVRGVDQVASALLNSSRIIRVDNNTITLGFKSELLRDKMNEEKPLRAVRSTLAKF
                     YRKDVMLICTAGVQGEDADNLEIIPNGLVDMTVRELGGKVRKKIVNKEKKD"
     gene            complement(10740..11252)
                     /locus_tag="PKL60_03240"
     CDS             complement(10740..11252)
                     /locus_tag="PKL60_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNW95182.1"
                     /translation="MKKRTPILPYLLLNVLVSAGTIILVLFLWQKLGGGSLPGLSSPK
                     LEAEPTQTAQQPTEAAPARQPAADAELSIEAVVGVGNRDLEYILVRNQSETAVDLAGW
                     ELSGSGGERYTFPALRLNKNGAVRLYSRTGTDSVIELFWNSAEALWETGEEIVLSAPD
                     GSAQCTYLLP"
     gene            complement(11584..12279)
                     /locus_tag="PKL60_03245"
     CDS             complement(11584..12279)
                     /locus_tag="PKL60_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95183.1"
                     /translation="MFIRVGLEVLGPGRVLAWGLDFPGCFAYGSDDAEAMLRFAGNLL
                     NFGAWVGLHTDTPWFQLGNVDFRLVEAYKGAELPVGKNADASSAFFEDDFRPLQQDEC
                     ANVLAVFRWQREELLAGLEFIPVELMGRKSPGETRTIREVVLRAARTELLYLNKLDLN
                     VPPLPDDAAPIDALQFSQEQITTRLMGLVENPVVLEVDGELWSCRKLARRLLWHQRVQ
                     IDEIKRLAGMPGA"
     gene            complement(12296..12850)
                     /locus_tag="PKL60_03250"
     CDS             complement(12296..12850)
                     /locus_tag="PKL60_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNW95184.1"
                     /translation="MEKERATYPIDIAGVHRELTLFEVKPGLRIAILNILGDVELVDA
                     CAKALAEKLSRLAYDLILTAESKSIPIAHMLAVETGKPYIVLRKECKPYMGNALEAET
                     LSITTGKPQKLFLDEKDRGLMEGKKVLILDDVISTGSTLQGMRLIVEKAGAQVAGEAS
                     ILTEGDRAEWMRIISLGHLPVFYD"
     gene            13097..14101
                     /locus_tag="PKL60_03255"
     CDS             13097..14101
                     /locus_tag="PKL60_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95185.1"
                     /translation="MFAVETEALGRIYKIKKTRKEAAHTLVALDNVNLSVNHGELFGL
                     LGPNGAGKTTLIKVLTTLLAPSSGCARVNGWDVARQPEEIRRQINMVSGGETSGYGLL
                     TVRENLWMFTQFYGIPSKQANERIMELLKIVGLEDRANTKSSNLSTGLRQKMNIVRGF
                     MTDPTVLFLDEPTLGLDVGAARDVRRFVRQWVDEDPTRTLLLTTHYMVEAEEMCDRVA
                     IINQGKILACDTPQNLKQGLRSEGIYSLELSALDPADFEELKGIDGFRQGTLTQIESG
                     AELELVLSDDSVLANLLSFLTARGSRIITLHKREATLEDVFINLVGKSMKDIENGAPD
                     "
     gene            14085..14927
                     /locus_tag="PKL60_03260"
     CDS             14085..14927
                     /locus_tag="PKL60_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95186.1"
                     /translation="MALQTKVTRNSGVRLFLQTVIGRAYPRVIGSQRDPAWLFFDIFL
                     PFLTMVGYVYIYRALKAPEEYIGFVVLGSAMTAYWINILWSMSSQLFWEKENGNLALY
                     VMSPTSLMAILFGMASGGLFSTTIRALVILVLGNLLFHVNYIITSFWALFLVFVLTMV
                     ALYGLGMMLSSLFMLFNREAFHFANLLEAPVMLFSGFYFPVSAFNFWVAAAASIIPLT
                     LGMDAIRQLVFPSTAQLGFLPVKLELIILAILSVVFLGGAFFSLRRMERLAIKQGRIT
                     DRLG"
     gene            14932..15816
                     /locus_tag="PKL60_03265"
     CDS             14932..15816
                     /locus_tag="PKL60_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95187.1"
                     /translation="METVKTTPTYEAKPGKLLTFRTSAWLGWMIESNWTDPFIFAVYS
                     IIRPILTAAILIVMYLVIRGGDFSSADFVNLYIGNAFYILVGAAIQRVSFGIIDDREH
                     YRTLKYIFIAPLQVPVYLIGRSVASILVSCFSVLFTLGFGILFLKMPIVLAEVKWGLF
                     VAAFFLGMAVMMCMGLILGGWTLIIKNNPWSLGDSMGAGLLIFSGAVFPLSVLPTPLQ
                     WIGNALPISYWLALIRRALVPEIGRAYPMYTNLSDLQLLLILLAFTLLYAVLAVVVYK
                     RFDIIARERGNIDMVSNY"
     gene            15816..16361
                     /locus_tag="PKL60_03270"
     CDS             15816..16361
                     /locus_tag="PKL60_03270"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNW95188.1"
                     /translation="MAHPSGFSAIHVLTPAGNLYLQASPDGLRRCAFCNRPAALPSTD
                     LSSARPDSPLLKAAVDQLGEYFEGACRSFSLALNLQDLPPFQTRVLTACAAIPWGKVL
                     SYAQLAFRIGAPGASRAVGAALANNPLLLFIPCHRVIGSDGALRGFAAPDGIQTKAWL
                     LQHEGLHIETGKVILQTGANP"
     gene            16358..17272
                     /gene="era"
                     /locus_tag="PKL60_03275"
     CDS             16358..17272
                     /gene="era"
                     /locus_tag="PKL60_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNW95189.1"
                     /translation="MSELIPGYKSGFVSIVGKPNAGKSTLANRLLGQPVAGVSAKPQT
                     TRKRQLVILTTDEAQMIFVDTPGLHEPKDKLSQFINSEALYALHDADIILFLADASQK
                     PDKLDEELAALVQEVQKDIPVLLALNKNDLADSKTLAANKALFESLLPGARVVSISAQ
                     TGNGIENLLRVLRELLPEGPQYYPPDQITETFERDIAAEMVRAACLTLLEEEVPYSIA
                     VRTDEYAERENGVIYVKATIFVERDAQKAIVIGKNGDMIKRIGSAARVDIEAASGQKV
                     FLDLTVKLRKDWKNDQAFLKELGLSQKS"
     gene            17275..17967
                     /locus_tag="PKL60_03280"
     CDS             17275..17967
                     /locus_tag="PKL60_03280"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNW95190.1"
                     /translation="MSAAAYVLLPALLCSAALNWHAAWRQDLSLYHATKPAVLIFLIA
                     LFIIWGGLEARALPFLAGLGLSLLGDIFLIPRSLAWFLTGLIAFFLAHLVYICGFNAA
                     PAPLLPTILIGAAALLVLTGLFIIIFQNTAKKPELKPMRRLFLPYAAVLVLMAASGAL
                     CLFRPEWPLQAAGMCAAGGLLFFFSDLMHGADRMGKRIPHGKFWIIATYHLGQFLIAF
                     GAAQWTQLSLPH"
     gene            18049..18384
                     /locus_tag="PKL60_03285"
     CDS             18049..18384
                     /locus_tag="PKL60_03285"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNW95191.1"
                     /translation="MSDETNPETESTQDDRLWATLAYIFSPLVPIVLLLLQKKRARPF
                     IKAHMAQALVFGSLVWLLIAILPQGLLERLVGVAAFSLAILWGVAASRGEHVNIPFIT
                     DFLKKMKWA"
     gene            18483..18959
                     /locus_tag="PKL60_03290"
     CDS             18483..18959
                     /locus_tag="PKL60_03290"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNW95192.1"
                     /translation="MSEQSEIPTTIDIPAEEPAHTVDPAEAITKDERTWALLAHLSIF
                     LNLFTGLLGTAAALVIYLVYKDKSKYVAYQAMQALIFQLAFFFGGGLLIGAVWLITGL
                     LSLILVGLILIPIALILSLLPIGCLVYAIYAAVETYHGKDFKYWLVGDWTRSTLTD"
     gene            19027..19284
                     /locus_tag="PKL60_03295"
     CDS             19027..19284
                     /locus_tag="PKL60_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNW95193.1"
                     /translation="MTTENLIRFYGVNWCGDCKRSKRVFSDTQVPYVYYDLDENPRAV
                     EFVKSVNQGMRRVPTIVFPDGSMLVEPSDGVLRTKLQEFQR"
     gene            complement(19275..19547)
                     /locus_tag="PKL60_03300"
     CDS             complement(19275..19547)
                     /locus_tag="PKL60_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95194.1"
                     /translation="MENLEKVKAKEPVDLDDFEGNIAGRLKPVGPDSFFRSALKTRLA
                     QSKIYARRKAAGALSVIWLIAALIALVTGELIYFCCRWRGNPASQR"
     gene            complement(19570..20127)
                     /locus_tag="PKL60_03305"
     CDS             complement(19570..20127)
                     /locus_tag="PKL60_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNW95195.1"
                     /translation="MDPLRAEAELVERAKTDPEAFGELYEKYIDRIYNYVYFRVGSVH
                     DAEDLTARVFLKALNNLNRYHFVGLPFSAWLYRIAHNLIANHHRDQARRREIPIEDMA
                     VVNIPDRLPAMDASIGRKQDAEALWRIINDLSPQKRELILLKFVEKLSNSEIAAVLNK
                     SESAIKSLYHRTLLEMRERIDRSDF"
     gene            20385..>21730
                     /gene="glyA"
                     /locus_tag="PKL60_03310"
     CDS             20385..>21730
                     /gene="glyA"
                     /locus_tag="PKL60_03310"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNW95196.1"
                     /translation="MNGFLIEKHLSDLDLDLDKLTALEGERQARRLIMIPSESSAPLA
                     VRELLASPYTNIYAEGYPRAETRTMSQAEILDYASQLGTYRRYSDLRYYKGVEYVDIV
                     EALARRRAAELFAANSLEADDLWVNVQPLSGAPANNAVYTALLKPGDTIMGMDLLHGG
                     HLTHGSPVNRSGLFYNAVHYTVNPQTERLDYDMIASLARETKPKIIIAGYSSYPWIPD
                     WEAFKQIAESVGAYLLADVSHISGLIAAKVCSSPVGIADVVTFTTHKTLCGPRGAVII
                     THDKELGEKFDKGVFPGEQGGPHINTIAAMALTFGLARSDEFLQLQQQTLRNASALAA
                     AFQKTGVKVPYKGTDSHLTLIDCKSFRGPEGVPLNGDLAARILDLVGIVVNRNTIPGD
                     RSALSASGVRFGTTWLTQRGFMESDFETIAEQITKLFRATSPYLMCAQNGKKTRAKV"
BASE COUNT         4448 a         6508 c         5997 g         4777 t
ORIGIN      
        1 cctggccccc acggccatcc ccacgccgct tcccaacccc acaagcgcgc ctacactgcc
       61 ccctgccctc accacgctgc ctgtggatga aagcttgcca gctctgctcg ggcatccgga
      121 agacctcccc agcggctata aacctggcag cctgggcagc attgatgagg atcgctttgc
      181 ctggattacg aacagaggtt ccgccagcca ccttctttct atcgaaaaag gtgataacgg
      241 cgtggaaggc tatgttgccg tcttcgttta tgaagacacg ctcgatccgg cactgtccta
      301 catggatgac ttaaacgaag tcacccgact gatccaggaa tacgggagtt cctacacctt
      361 cgaatttgac accattgacg atgtgggcga aaaggacatg gcgtatgtga tgtcagcgag
      421 ttccagctcg gaggcaaaca tcgcggtggg attcgtgcgc tgcaatctgt atgtgcgcat
      481 cagttttatg aacagcaagg accgcgcgat gattatcagc tacgcgcaaa agttggacga
      541 ccgcattatc gcgcttggct gccagtacta aacgcaagtc tccatcgcct tcccttgcac
      601 gcccaagctg agctttggcg ctaattccaa attcagagcc ggactgcgcg tccggctcag
      661 tgattccccc ttttggcggg taagccatat gataaaattc tggcatgaat gcccgggtcg
      721 tctttatggg ttcgcccgat ttcgctctcc ccaccctgga agaactgcat aacaacacgc
      781 aggtcgttgg cgttgtcacc cagccggacc ggccggccgg gcgcgggcgc caacccatgc
      841 cctgccctgt caagcaattg gcggatcacc tgcggatacc ggtcttccag ccgctctccc
      901 tgcgtaaacc cggcgcgctg gagcagctgg aagcctggca gccggacctg attgtggtgg
      961 cggcgttcgg tcagatcctc ccgaaaagcg tgctggcgct cccgccgaaa ggctgcctca
     1021 acgtgcacgc ctcgcttctg ccccgttggc gcggcgcgtc ccccatccag gctgccattc
     1081 tggcggggga tgaacgcagc ggcgtgacca ttatgctgat ggaggaaggt ctggataccg
     1141 gcccaattct gacgcaaagg actgtgccta tccggccggg taccaccggc gcggaacttt
     1201 cgcagcagct ggcttttctg ggcgccgcga cgctgacgga aattcttccc tcctatctct
     1261 ccggcatgct cgcgccgctg atgcaagcgg acagcctggc cacatacgcg ccgatgctca
     1321 aaaaatcgga cgggctgctg gatttccatc aacccgcgct caccctcacc cgcaaggtgc
     1381 atgcctattt cccctggccc ggaacatatt tcgtcttgaa cgggaagcaa atcaaggtgc
     1441 tcgacgcgca cccgcacgat acctttgaga gtgaaatcga agggcattac attgtcaatg
     1501 gtttgcctgc tgtcggtacg tcggaaggct tgttggtgct ggacaggcta cagccggagg
     1561 gcaaacgcgg catgcccggc agcgattttc tgaacggtca gcccggctgg ctgtaagttt
     1621 cgtccataca aagattcctt ggtacttatt gagcgggatt cttatttgtc attgcgagcc
     1681 cccggtgtaa tcacccatag atcacctgga taaacaagga gggggctcgg caatctgcct
     1741 gaattccatt gcatagcaga attattcacc aggcgtttat gatgttgaaa ggcagactgc
     1801 cacgaccctt tgccaatcca tatccctaat aaccagccat aaccttgtgg caaagggtct
     1861 cgcagtgacc acgaagctgc gctcgcgata acgaatgatt cggcattgcg aaccgttttg
     1921 ccgccaaacg tcgagctggc cagatgaggc atcaaggcaa aacggtgtgg caatctgcct
     1981 gtgtgcatgc ttgcctgaat gcctgtctct cagcaatggc gaagctgagc caacagggag
     2041 atcaccgcgc tgcgctcgcg atgacgaacg attcggctcg cggtggcgcg gcttgtcatg
     2101 ctaagcccca gcgaagcatc tgatcagaat agaggaaagg ctttgcgctg cacttaagag
     2161 tgacaagtaa aatttctcaa ctcgaaatac ccacctggct ttgcctaccc ccctgaaaac
     2221 aacgctactc ctccgggaag aagttcctgc gccactcgta gagcaagttc agcgcctgga
     2281 tcggggagag cgaattcaaa tccacctttt tgaaggcatc cataagcgga ttattctccg
     2341 ggaacaggcg cagctgcgat gagtccggca cctgggccgg gatagtcttc ccggacgtgg
     2401 cttcgagctg cttaagcagc tcgttcgcgc gttgaatcac ggggcctggc agcccggcca
     2461 actgcgccac gtggatgccg tacgagcggt ccgccccgcc gggcacaatc ttgtgcagga
     2521 agaccacctt gccctcggat tccgtcacgg ccacgttgta gttgcgcacg cccggcagca
     2581 tctccgccag ctcagtcagt tcgtgataat gcgtggcaaa cagggtaaag ggcttgagtt
     2641 tggggtgact gtgcagatac tcaataatcg cccaggcgat agaaagcccg tcgtaggtgc
     2701 tggtgccgcg cccgatttca tccaggatta acaggctgcg aggggtggcg ttgttcagga
     2761 tatttgccgt ctcaatcatc tcgaccataa aggtggattg accggcgtga atcgcatcct
     2821 gagcgccgat gcgggtgaaa atgcggtcta ccagcccgat ttccgcagac tccgccggaa
     2881 caaagctgcc cacctgcgcc agcaggacaa tcagcaccac ctgacgcaga aatgtggatt
     2941 taccgctcat gttcgggccg gtgatgacgc gcacaatctc gccttcctca aacaccgcat
     3001 cattggcgat gaagcgctca ccggtgcgca gccgctccac caccgggtgc cttccgccaa
     3061 taattttcag ccctttttcc gcgcgcaccg tcggtcggac gtagttgttc agcgcggcgg
     3121 cagccgccag cgagagcacg cagtccaacg ccgccagcgc tctggcgctt tccagcagca
     3181 gcgcggctga actggccacg cgctggcaca cggacctaaa gacgcgcagt tccacctcgt
     3241 ggatttgcgt ctctgcattc agaacgaggg actcgtattc tttcagttcg ggcgtgatga
     3301 agcgttcagc gttcaccagg gtttgcttgc gcaagtaatc ggcgggggcg ttgtccgcgc
     3361 ttccccggct gatttcaatg taatacccaa atactttatt aaagcccacc cgcagcgtct
     3421 tgatgccggt ccgtttgcgt tcctcgccct caagattgtt aatccactcg cgcgcgtgct
     3481 gcgacgagga aacaatcgcg tccagctcgg cggaaaagcc gggtttgatg acgccaatat
     3541 tcgccagcgt cgctggcgga tcatctgcga ttgcttcgtc cagcaggctc agcacatccg
     3601 gatgttcgtg cagttctgcc agaagggccg cgagcgccgg ctcggtgggc aagagctcgc
     3661 gcagtttggg caaaaggcgc aaatcctcgc gcaggctcaa cagctccggc gggcggatgc
     3721 tgttggtaca tacgcggtta accagccttt ccatatcgtg aaagtccgcc aaaaagtgcg
     3781 tgagctcgtt gcgccgcacg ccgtcacggt aaaaagactc gaccctatcc aaccgggctg
     3841 tgattgccgc caggttcagc agcggtttgt tgacccattg gcgcagcatg cgtttgccca
     3901 tcggggtctt ggtttggtcc agcacccaca gcagcgagcc ttcgcttttc gcgtcccgca
     3961 gcgtttcggt cagttccagg ttgcggcggg tttcggcatc cagcaccata aagtcgccca
     4021 gactgtaggt gccaaagctt ttcagggtca ggagggcatc cttatgcgtt tcctgcaggt
     4081 actttagcag cgccccagcc gcgcgcacgg tgagcggtaa attcttgagg ccaaaaccgt
     4141 ccaaactggc caccccaaaa acgctcttaa gctgttcttc ggcgcgcccg ggttcaaaat
     4201 accaggcggg gacgggcgtg agcctgcccg gaaagctttc cgacagctcc tgcccctcag
     4261 gtcgcaaggt ctcggccggg ttgatgcgaa taagttccgc ccgcaaggcg ttcaggttca
     4321 cttccaggtc cagttgagtg ccggtgaatt cgccggtggt gatatccaga taggcgatgc
     4381 cggcgcggtt ttcctgcaag cagaccgcgg caaggtaatt gtttctgccg gcgctgagca
     4441 gccccggctc taccacggtg ccgggcgtga ccacccgcac cacctcacgc gtgaacaggc
     4501 ctttatccgg ctgcccgccc acctgctcgg cgatggcgac ccggtagccc ttttcaatca
     4561 gccgcccgag gtaattatcg gcagcatgga aaggaatgcc cgccatgggc acccgcacgc
     4621 ccctggccac cgggcgggag gtcagaacga tatccagcgc ttccgaagct ttgcgcgcgt
     4681 cgtcgtcaaa ggtctcgtaa aaatcaccca gtctgaagaa cagaatcgca tccggatact
     4741 gttttttgat atccaggtac tgctgcctta acggggtgat atcctgcgcc atactgcgcc
     4801 ttaccggagg gtctccgcca cgtaagccgg cacctgatcg agggcttccg aaattttgcc
     4861 cgggtccttt ccaccggctt gcgccaggtc gggttttccg ccgccgcctc cgccaaccac
     4921 tgccgcagcg cgtttgacca ggtcgccggc tttcaggccg cgtttgatca ggtccggcgt
     4981 gactgccgcg ataatcagcg gcttatcatc caggacggaa gccaaggcaa ccaccccgga
     5041 agggtagcgc tgccggaact ggtcagccag tccgcgcagc atttccgcgc ctgcccccgg
     5101 cacctgcgcc gtcagaaccg gcaccccgga gatgacccgc acctggtcca gctggcggtt
     5161 gaaggcttcc tgcgcgattt gagcgctcag ctgttcgatg cgcttctctg ccttttcaag
     5221 ctcatcttgc agcgcggtca acctcacggg cagctccgcc ggggaagcgc ccaccatctg
     5281 cgcgctctgc tgcagcaggt tcaaccgttc ccgcgcgaac gcgtaagccg ccagcccggt
     5341 caccgcttcg atgcggcgga taccggcagc gacgctgctc tcgctggtaa tcaggaacag
     5401 cccgatttcg ctggtgttgt gcacgtgcgt accgccgcac agctcgtagg aaatggtttc
     5461 ctcgtcccca attttgatag tgcggacttc ctgtccgtat ttctcgccaa aaagcgccat
     5521 cgcgccttcg ttaatcgctt tctccaaggg tttgactgtg atgttcaagt catactcgcc
     5581 caggacggct tggtttacgc ccttttcgat gcgcagaagc tgctcgtggc tgatggcgct
     5641 ggggtagttg aagtcaaagc gcagcctgtc aggcgcgacc aatgagcccg cctggcgcac
     5701 ctggtcgctt agcgttttgc gcagctcggc gtgtaataaa tgcgtagccg tgtggttgcg
     5761 cataatggct tgccggcgcg gtttatccac gcgcgcgaca gcattttcgc cgacggttgg
     5821 cagcccaaac accacccgtc cgacgtgcac aatcacgccc gcggcgggtt tacgcagccc
     5881 ggagacttcg atttcccagc ggccggccgg gtcggaagtg attttgccgg tatccgagac
     5941 ctgaccgccg gattcaaggt aaaaactggt atccccaagc agcacttcca cctcatcgcc
     6001 cgcctgaacc tgccgcacag gctgcgcgtt ctgaaagagc gccagcacgg tggagggaac
     6061 cacgtaccgg tcgtacgggt cgtaggcgca gccagcctcg cccagcgcgt gattcttaat
     6121 taattcctgc aggacgcgtt ggtagttttc cgcgtcgcgt ccgcccatat ccccaaacgc
     6181 tttgcctgcg ccgctggcag cgcggtgggc ttccatgctc tcaaagaagc cgctttcatc
     6241 cacgcccaga ccttcctcac cgaggatgtc gcgcgtgatt tccaatggga agccgagcgt
     6301 cgcgtacagg tcaaaggccg ccttgccgtt taagacgctc tcaccgcgcg agcgcatgcc
     6361 ggcaatcagg cggctgagct cggcaaagcc cgcgtcgagc gtttcggcaa agcgctgttc
     6421 ctcgcgtgtg agcgtgtcca gaatcaggct ttcgctgcgt ttcaattcgg ggtaagcgtc
     6481 ctggtagatg ctgatgacgc gcgcggcgac tttggccatg aagggctctg tcaggcccag
     6541 cttgcgcgca aagcgcgctg cccggcggat aatcatccgg caaatgtagt tgcgcccaag
     6601 gttgcccggg atgacgccgt ccgcaatcag gaaagctgag gcgcggacgt ggtcggcgat
     6661 gacgcggtag ggggtaagat tttcggcgcg ctgcgcatcg gtttgcccgg tcagcagctg
     6721 cacttcatcc aggagcgggg aaagcaaatc cgtggcgtag ttgccgcgca caccctgaat
     6781 tatcgataca atgcgctcga agcccatgcc ggtatccaca tgctttttgg gcagcggcgc
     6841 cagttccgtc ggggacagac ggttgtactg gataaaaacg ttgttccaga cttccgtcca
     6901 gcgcccgcag ctggcgttga cgtcgcagac gtgttggggg tcatccttct gcgtgcaggc
     6961 ttccggcccg aagtcatagt ggatttcgct gcaaggaccg cagggcccgg tttccgccat
     7021 ctcccagaag ttatccttgc gcccggagaa gaccagatgc gtggggtcaa agccgggttg
     7081 gctcagccag accgcggcgg cttcttcatc gtccggtatc tgaccgtatt cgtctttgaa
     7141 ggtcgtcgcc cacaagcggc ttttatccag tccccagact tctgtgagca gttcccagga
     7201 ccaggaaagg gcttcttttt tgtagtaatc cccaaaagac cagttgccga gcatctcgaa
     7261 gaaagtatgg tgggtgttgt cccgtccgac ctggtcgagg tcgttgtgtt ttcccgagac
     7321 gcgcatgcac ttttgcgagt ctaccgcgcg ggtatatggg cggctgtccg tgcccaaaaa
     7381 gacgtccttg aactgcacca tgccggcgtt ggtgaagagc agggtggaat ccccgccggg
     7441 gacgagcgaa gcgctgggga cgtgggtgtg tccgcgctcc gtgaaaaagt cgataaagga
     7501 ctgtcgaatt tggttaccgg ttaaaggcat agagaactcc gtctgaaggg tttacagagt
     7561 gaaaattata ctgtatttcg accggcagga acgggaaccg ggacgcctca tcaaacctgt
     7621 aacggaaata gacgctgcca gaaaatagaa gtaagctttg cggtttaccg aaaccgcgag
     7681 aaagcccagg gtgagcccaa agggaagaaa gtgactgaaa gtgtaaggga taggtctcct
     7741 gtaaagtgca aagacacagg gtacgccttc ccgggagaat tcatcgccgt taaaaactgg
     7801 cgggcgtttt tccagccggc ggtaaggatt gattctgggt ttcaggcgta tgggcaaagc
     7861 atcccaaagg aatcaactgc cgcactgcct gccccatcca ggatgtttcg ccctattagc
     7921 aaacgacgca tgcaaacctg ctaatgaccc ctcaccgcct gggctgcgct cgccagcttc
     7981 gccccagaaa gctcaaaaaa aacctgtaca cccaagcaaa accaaaccat agtgtgacaa
     8041 agaaaaacgc ggagcttgct ccgcgctctc actcatcaac aatctcacta ctccatgctc
     8101 tgcctgccag ccagcgcccg cagcaaggtg ttttggtcag cgtactcaat atccccgccg
     8161 accggcagcc cgcgcgccag gcggctgacc ctgatcccca atggcaagat cctctgctgc
     8221 aaatacagcg cagtcgcgtc cccttccatg ctggggttgg tcgcgataat cacctcgcgc
     8281 accgttccac ctttcaggcg ttccaacagc gggaaaatct tgatctggtc cgggccaatc
     8341 ccctcaatcg gcgagagcac gcccagcagc acatggtact tgccgttaaa cgcgccggtg
     8401 cgctcaatcg cgagcacatc cagcggctct tccaccacac acaggatgct ttcatcccgg
     8461 ttgtccgaag cgcaaatctc acactggtcc tgcccggcgc tggtgacgtt aaagcaggtg
     8521 cggcagtgct gcgtctggga ctttagctgt tccaatgcct ctgccagctg cagtgaactt
     8581 tcgtccggcg cctttagcag gtaaaacgcc agccgcgaag cagtcttcgg gccgattccc
     8641 ggcagcctgg cgaaggcgtc aatcaggttc tgaaccggat cgggcagagt acgcatcagg
     8701 gttttacctt cccatgccca tgccgcccag caggttggca aagggtgcca ttttctcttc
     8761 ggaaagggcg cgggtgccgt ccaaggcctt attaatagcg ataagcagca aatcctgcag
     8821 catttccacg tcggcttctt tcaaaacatc ttcctggatg ctgacagcca cgcatttctg
     8881 gtcgccggtc atcgttacgg tcaccgcgcc gccgccggcg gtctcagtca gctgcgtttc
     8941 ggaaatctca gcctgcgcag ctgccatttg ttcctgcaat ttggcgattt gcgccatcat
     9001 gttcccgcct ggtctggcgg atggtccagc attgtatcct tttgccatct taatccttct
     9061 tttctttgtt cacaatcttt ttccgcactt tcccgcccaa ttcgcgcacg gtcatgtcca
     9121 ccagcccatt ggggatgatt tcaaggttgt ctgcgtcctc accctgcacc cccgctgtgc
     9181 agataagcat cacatctttt ctgtagaact tggccagcgt gctgcgaacc gcgcgcagcg
     9241 gcttttcttc attcatcttg tcgcgcaaga gctcgctctt gaagcccagc gtgatcgtgt
     9301 tgttatccac gcgaataatt cgcgaggaat tcagcagcgc gctggcaacc tggtccaccc
     9361 cgcgcacatc gcgcttgatt ttgtcccagt cctgcagcag attttccagg ttcagctcgc
     9421 cgctttcccg gggagcggat tccgcggacg cgctggtctc gggttttctt tcccccacct
     9481 tatcttccgt tttgcgatcc ttttgggaat cccccgctgt cttgctttgt tcaggcgctg
     9541 gcgttgattt tcgggcaggc gtttccgagg cgggttcttt ctgcccgggc ccctgagcag
     9601 aatttcgcac aggcgcaaga gcagccggct cagctgccaa aaccggcgca gccgtggcgg
     9661 aaacggagcg ggcaaaagcc aattccaaac tcaagcccgg ataccaattc gccttgagct
     9721 cggcggcagc tctactgaat tcgtcaattc cggaaatcaa atcccctgcg ccaaacgcgc
     9781 cggcctgttt gttcaccacg ctgcgcacct caggcagcaa ttccaggtct ttgccgccgt
     9841 tcatcttgac caccagcagc gaacgcaagt aatccaccac ctggcgggcg aattggcgcg
     9901 ggtcggcgcc gccgtccagg gcgccgtgga tgagctccaa gccgcgcgca ctgtccttat
     9961 cctgcacagc ctgcaccagg tccatgacgg tctggctggt ggccgtgcct aaaatatcct
    10021 gggccgcggc cagggtaacg cgcccaggca gcgaagcgag ctggtccacc agtgagatag
    10081 catcgcgcat actgcctgtg gactgacgcg cgatcaggct caaggcatcc tcatcgatgc
    10141 caataccttc ctgctcacaa atctgacgca gtttccctac aatctggttc agggggatgc
    10201 gccggaattc atgccgttgg cagcgcgaaa gcaccgtggc ggggattttg tgaatttcgg
    10261 tggtcgccag gatgaaaatg acatggctgg gcggctcttc cagggtcttc agcagggcgt
    10321 taaaggcaga agtggaaagc atgtgcactt cgtcgatgat ataaactttg aacttgccct
    10381 ggctgggtgc gaaattgatt gtatcgcgca gttcgcggat atcctccacg ctggtgtttg
    10441 aggccgcgtc aatctcgatc aggtccagaa agcgcccctc gttgaccgcc ttgcagttct
    10501 cgcaggtatt gcacggacgg gcggcagggt cttctgccag gcagttgacc gccttagcca
    10561 gcaagcgtgc ggaagtggtt ttgccggttc cgcgagggcc ggcaaacagg taggcctgat
    10621 tgacgcggcc ggagatgacc gcgttgcgca gcgtctgaac gatatgttcc tgccccgcga
    10681 cttcctccca ggaagccggg cgccaggtgc gataaagggc ttgagtcatg tcgatttccc
    10741 tacggtaaca gataagtaca ttgcgcgctg ccgtcgggcg cggataacac aatttcctca
    10801 cccgtctccc acagcgcctc agccgagttc caaaacaatt cgattaccga atccgttccg
    10861 gtgcggctgt acagacgaac tgcgccattt ttattcaatc ggagcgcggg aaaggtgtac
    10921 cgctccccac cagaaccgga taattcccat ccggccaggt ccactgcggt ttcgctctgg
    10981 ttgcggacca ggatgtattc caggtcgcgg ttcccgaccc cgacaaccgc ttcgatgctc
    11041 aattccgcat ccgcggcagg ctgccgcgcg ggggcagcct cggtgggctg ctgcgcggtt
    11101 tgggtgggtt ccgcttcgag cttgggcgag ctcaggccgg gcaggctgcc tccaccgagc
    11161 ttttgccaca ggaacagcac caggattatc gttcccgcgg agaccagcac atttagaagc
    11221 aaatacggta gaatgggggt gcgctttttc atatttgtgt gggtaatgat agcataaacc
    11281 agacgccttt agagagtttg gcgccgggtt gtttgggcaa gatcacaacc tgatctttca
    11341 taaagtgtcc acgcaatatc cgcttccaat ttctcgggaa agtttaatat tcagaaccaa
    11401 gttattaaca aattgataac attggcttta ttcagattta attgacgcgt gttaatattc
    11461 aaatcagatt tgaacactta ttgtttctct cctttcaaat ttgttcaaat caggatcatg
    11521 aagtgacgaa acactatgtt gatcgaaagt aagccaggcg caggtgaggc gcctggtttg
    11581 cttttaagcc cccggcatac cagccaggcg tttgatctcg tctatctgca cgcgctgatg
    11641 ccacagcagc cgccgcgcca atttccggca agaccataat tccccgtcaa cttccaggac
    11701 cacagggttt tctaccaggc ccatcaaacg cgtggttatc tgctcctgac tgaactgcaa
    11761 agcgtcaatc ggcgcagcat cgtccggcaa aggcggcacg ttcaggtcaa gcttgttcag
    11821 gtacaagagc tccgtgcgag ccgcgcgcag cacaacttcc cggatcgtcc tggtttctcc
    11881 ggggcttttt cgacccatca gttccacggg gatgaattcg aggccggcca ggagttcctc
    11941 gcgctgccag cggaaaaccg ccaaaacgtt cgcgcattcg tcctgctgca aaggacgaaa
    12001 atcatcctca aaaaacgcgc tggaagcatc cgcgtttttt ccaaccggca attctgcccc
    12061 cttgtaggcc tcgaccaggc ggaaatccac attccccaac tggaaccagg gcgtgtcggt
    12121 atgcaagcca acccaggccc cgaaattgag caaattgccg gcaaagcgca gcatagcctc
    12181 ggcgtcatca ctgccgtagg caaagcagcc cgggaagtcc agaccccagg cgagcacgcg
    12241 cccggggccc aacacttcca atccaacccg gatgaacatt tatacttttc ttcagctaat
    12301 cgtagaagac aggcaggtgc ccaagggaga tgatccgcat ccactccgcg cggtcgcctt
    12361 cggtgaggat ggaagcctcg ccagcaacct gcgcgccggc tttttccaca atcaggcgca
    12421 tgccctgcag ggtggaacca gtgctaatca cgtcatccag aattaacacc ttttttccct
    12481 ccatcagacc gcggtctttt tcatccagaa acaatttctg cggtttaccc gtggtgatgg
    12541 agagagtttc agcttcgagc gcgttgccca tatacggctt gcactctttg cgcaatacaa
    12601 tgtagggttt gcccgtttca acagccagca tatgggcaat cggaatgctc ttggactccg
    12661 cggtcaggat aagatcatag gcaagccgcg aaagcttttc cgccagggct ttcgcgcagg
    12721 catccaccag ctccacatcg ccaaggatgt tgagaatagc aatgcgcaga ccgggtttta
    12781 cttcaaacag ggtgagttcg cggtgcacgc ctgcgatatc gattgggtaa gtcgcgcgtt
    12841 ctttttccat ggtgcctcct gagctggata cgatacctgg attctaaacc aaatccactg
    12901 gcagggcacg atgaattcct tttcacacaa aaatccccct tagaatcagc tagtgccagg
    12961 gacgccgacc ccggcactag gtttaaggag gagaagaaga agaagagaag tcgccctttc
    13021 aatcaatatc atatacaata tatgcctgta ctgcttgacg cctgacctac gtttacccta
    13081 cgaacctgag gatagcatgt ttgctgttga aaccgaagca ttaggacgaa tctataaaat
    13141 caaaaagacc cgcaaagaag cagcccacac gttggtcgcg ctcgacaatg tgaacctgtc
    13201 tgtaaaccac ggcgagctat tcggcttgct cggaccaaac ggcgcaggca agacgacttt
    13261 aatcaaggtg ctgaccaccc tgctggcccc cagcagcggt tgcgcgcgcg tgaacggatg
    13321 ggatgtcgcc cgtcaaccgg aggaaatccg gcggcagatc aatatggtgt ccggcggtga
    13381 aacctccggt tacggcttgc tgaccgttcg cgaaaacctg tggatgttca cccagttcta
    13441 cggcatccct tccaaacaag ccaacgagcg catcatggag ctgcttaaaa ttgttggcct
    13501 ggaagaccgt gcgaacacca agagcagcaa cctcagcacg gggctgcgcc aaaaaatgaa
    13561 catcgtgcgc ggctttatga ccgacccgac cgtgctcttc ctggatgaac ccaccctcgg
    13621 gctggacgtc ggcgcggcgc gcgatgtgcg gcgtttcgtc cgccagtggg tggatgagga
    13681 cccgacccgc acactcctgc tgaccacgca ttatatggtc gaggcagaag aaatgtgcga
    13741 ccgcgtggcg attatcaacc agggcaaaat cctggcttgc gatacccccc agaacttgaa
    13801 gcaaggcttg cgcagcgaag ggatttactc tttggagcta agcgccctgg acccggcgga
    13861 ttttgaggaa ctcaaaggga ttgacggctt ccgccagggc acgctcacac aaatcgaaag
    13921 cggcgcggag ctggaactgg tcctttccga tgacagcgta ctcgcgaacc tgctcagctt
    13981 tttgacagcc agaggcagcc gtatcatcac gctgcataaa cgcgaagcca ccctggaaga
    14041 tgtttttatc aacctggtcg gcaaatccat gaaggatatt gaaaatggcg ctccagacta
    14101 aagtcacgcg caacagcggc gtgcgcctgt tcctgcagac agttatcggc agggcctatc
    14161 cgcgcgttat tggctcccaa cgggacccag cctggctgtt tttcgacatt ttcctgccgt
    14221 tcctgaccat ggtcgggtac gtttacatat accgcgccct gaaagcgcct gaggagtata
    14281 tcggcttcgt cgtgctcggc agcgcgatga ccgcctactg gataaacatc ctctggtcga
    14341 tgtccagtca gctgttctgg gagaaagaaa acggcaacct ggccttgtac gtcatgtcgc
    14401 ccacctcact tatggcgatt cttttcggca tggccagcgg cgggctgttt tccaccacga
    14461 ttcgcgcgct ggtcattctg gttctcggta atctgctttt ccacgtgaac tacatcatca
    14521 ccagtttttg ggcgcttttc ctggtttttg tgctcaccat ggtcgcgctt tatgggctgg
    14581 gcatgatgct gtcatccctg ttcatgcttt tcaaccggga agcctttcac ttcgcgaatt
    14641 tgctggaagc tccggtgatg ctcttttccg gtttttactt tccggtgagc gcgttcaact
    14701 tttgggtcgc ggcggctgcc tccatcatcc cgcttacact gggcatggac gcaatccggc
    14761 agcttgtgtt tccttccacg gcacaattgg gcttcctccc ggtgaagctt gagttgatta
    14821 tcctggcaat tttgtccgtt gtctttctgg gaggggcctt tttctcgctg cgccgcatgg
    14881 aaaggctggc aatcaagcaa ggtagaatca cggacagatt gggttaacac aatggaaacc
    14941 gtaaaaacga ccccaactta tgaagccaaa cccggcaagc tgctcacctt ccgcaccagt
    15001 gcctggctgg gctggatgat tgagtccaat tggacggatc cctttatctt cgcggtttat
    15061 tccatcatcc gccctatctt gacagcggca attctgattg tgatgtacct tgtaatccgc
    15121 ggcggagatt tcagcagcgc ggattttgtg aatttgtaca tcggcaacgc cttttacatc
    15181 ctggtcggcg cggccatcca gcgcgtttcc ttcggaatta tcgacgaccg cgagcattac
    15241 cgcacgctca aatatatttt catcgcaccg ctgcaggtgc ccgtgtacct gattggacgc
    15301 agcgttgcca gcattcttgt ctcctgtttt tccgtgctgt ttaccctggg ctttgggatt
    15361 ctctttctga aaatgccaat tgtactggct gaagtgaaat ggggtctctt tgttgcagct
    15421 ttttttctgg gcatggccgt gatgatgtgc atgggcctta tcctgggcgg ctggacgttg
    15481 atcatcaaga acaacccctg gtcgctggga gatagcatgg gcgccggctt gctcattttt
    15541 tccggcgcgg tgttcccgct cagcgtcctg cccactccgc tgcagtggat cggaaatgcg
    15601 ctccccatca gttattggct ggcgttgata cgcagagccc tggtgcccga gattggcagg
    15661 gcttacccga tgtatacaaa tctttctgac ctgcagcttc ttcttatcct gctggctttc
    15721 acgctgctgt acgccgtcct ggctgtggtg gtctacaagc gtttcgatat catcgcgcgc
    15781 gaacgcggca atatcgacat ggtgtcgaat tattaatggc gcacccctcc ggcttttccg
    15841 ccattcatgt tttgaccccc gcagggaacc tttatctgca ggccagcccg gacggcttgc
    15901 gccgctgtgc tttttgtaac cgccccgcag ccctgccctc tacagacctg tcctccgccc
    15961 gcccggattc gcccctgcta aaagcggctg ttgaccagtt gggcgaatac ttcgaaggcg
    16021 cctgccggtc cttttcgctt gcgctcaacc tacaagacct gccccccttc caaacccggg
    16081 tgctgactgc ctgcgctgca attccctggg gaaaagtgct cagctacgcg cagctcgcct
    16141 tccgcattgg cgccccgggc gccagccgcg ccgttggtgc cgcgctggca aacaacccgc
    16201 tgctgctctt cattccctgc caccgagtta tcggctcgga cggagccctg cgcggcttcg
    16261 cggcgccgga tggaatccaa accaaagcct ggctgctgca gcatgaaggc ttgcacatcg
    16321 agactggaaa ggtcatctta caaacaggag caaatccatg agcgaactta tccccggcta
    16381 caaatccggt tttgtatcca tagtgggcaa gcccaacgct ggaaaaagca ccctcgcgaa
    16441 ccgtctgctt gggcagccgg tggccggcgt ctccgccaaa ccgcaaacca cccgcaaacg
    16501 ccagctggtc atcctcacga ccgatgaagc tcagatgatc tttgtggata cccccggtct
    16561 gcacgaacca aaagacaagc tgagccagtt cattaattcc gaggccctgt atgctctgca
    16621 cgacgcggat attatccttt ttttggcgga cgcttcgcaa aagccggaca aattggacga
    16681 agagctggcg gcgctggtgc aggaagtcca gaaagatatt ccggtgctgc tggcgctgaa
    16741 caagaacgac ctggcggaca gtaaaacatt ggccgccaac aaagcgctct tcgagagcct
    16801 gcttcccggc gcgcgggtcg tttcgatttc tgcgcagacc ggcaacggca tcgaaaacct
    16861 gctgcgcgtg ctgcgcgagc ttttgccgga aggcccacag tactacccac ccgaccagat
    16921 cacagagact ttcgaacgcg acatcgcagc ggagatggtg cgggctgcct gcctgaccct
    16981 gctggaggag gaagtgccct acagcattgc tgtgcgcacg gatgagtacg ccgagcgcga
    17041 aaacggcgtt atctacgtga aagcgaccat ttttgtggag cgggacgcgc aaaaagcgat
    17101 tgtcatcggc aaaaacggcg acatgattaa gcgcatcggt tccgccgcgc gcgtggatat
    17161 cgaggccgcc agcggtcaga aggtgttcct ggacctgacc gtgaagctgc gcaaagactg
    17221 gaaaaacgac caggcatttc tgaaggagct gggtctctcc cagaaatcct gagcatgtcc
    17281 gcggcggctt atgtgctctt accggcattg ctgtgcagcg cggcgctgaa ctggcatgcc
    17341 gcctggaggc aggacctcag cttgtaccac gcgacgaagc ctgctgttct gattttcctg
    17401 atcgcgctct tcatcatttg gggcggtctg gaagcgcgcg ccctgccctt tctggccggg
    17461 ctggggcttt ctttgcttgg ggatatcttc ctgattccgc gctcgctggc ttggttcctg
    17521 accgggctga ttgcgttctt cctggcgcac cttgtttata tctgcgggtt taacgccgcg
    17581 cctgccccgc tcttgcctac aattctgatt ggcgcggcgg ccctcctggt ccttacaggc
    17641 ttgttcatta taattttcca aaacaccgct aaaaagccgg aactaaagcc aatgcgtagg
    17701 ctgtttttac cctacgcggc ggtgctggtt ttgatggctg cctcgggcgc cttgtgcctc
    17761 tttcgccccg aatggcctct tcaggcagca gggatgtgcg ccgctggcgg tctgcttttc
    17821 tttttctccg acttgatgca cggcgcggac agaatgggca agcgcatccc gcatggcaaa
    17881 ttttggatta tcgccaccta tcacctcggg cagtttttga ttgcatttgg cgccgcgcag
    17941 tggacgcagc tcagtttgcc ccattgatgc agcgcgcacc gcaggcaaag cccgccgctc
    18001 cgcgtgttat aatggcgcta atctcaggtc tgcacaggag ttcccggtat gagcgacgaa
    18061 accaatcccg aaaccgaaag cacgcaggac gaccgccttt gggccacgct tgcctacatc
    18121 ttctcccccc ttgtacctat cgtgctgctg ctgctccaaa agaaacgcgc gcgccccttt
    18181 atcaaagccc acatggcgca ggccttggtg tttggctcgc ttgtatggct gctgattgct
    18241 atcctgccgc agggactgct ggaaaggctc gtgggagtgg cggcattctc ccttgcaatc
    18301 ttatggggcg tggcagccag ccgcggcgaa catgtcaata tcccgttcat cacggacttc
    18361 ctgaaaaaga tgaagtgggc ttgattcctg ccgcgcgccc aaagaccaaa gattttctac
    18421 agtccgatcg cctgagcgcg tgtaagatat tggaaatctt caacaacaga aagtaggaaa
    18481 caatgtcaga acaatcagaa attcccacta ccatagacat tcccgcggaa gagcctgccc
    18541 acacggtgga tcccgcggaa gcgataacga aagatgaacg cacctgggcg cttctggcgc
    18601 acctgtccat ttttctcaat cttttcaccg gattgctggg cacagccgcg gccctggtca
    18661 tctatctggt gtacaaggac aaatccaagt acgtggcata tcaggctatg caggccctga
    18721 tcttccagct ggccttcttc ttcggaggcg gtttgcttat cggcgcggtc tggctgatca
    18781 ccggattgtt gtcccttatc ctggttggct taatcctgat ccctatcgcg ctgattctga
    18841 gcctgctgcc catcggctgc ctggtgtatg ccatttacgc cgcggtcgag acctaccacg
    18901 ggaaggactt taagtattgg ctggtcggcg attggacccg ttccacgctc accgactaat
    18961 tttgtcagag caaatcaggc agccccaaaa ccaggggctg cctggcaagt taccggggtt
    19021 atcacgatga ccacagaaaa tctcattcgc ttttacgggg tgaactggtg cggggattgt
    19081 aaacgctcca aacgggtatt cagcgatacg caggtgcctt atgtttatta tgacctggat
    19141 gaaaaccccc gcgctgtgga gtttgtgaag agcgtgaatc agggcatgcg ccgcgtccct
    19201 accatcgttt tcccggacgg ttccatgctg gtggaacctt ccgatggagt tttacggaca
    19261 aaactgcagg aatttcagcg ctgagaggcg ggatttcccc gccagcggca gcagaagtaa
    19321 atcagctccc ctgttactaa cgcgattagc gcggcaatta accagatgac gctcaatgcg
    19381 cccgctgctt tccggcgggc gtagattttt gattgcgcca ggcgggtttt caaagctgaa
    19441 cggaagaatg aatccggtcc caccggcttc agacggccgg caatgttccc ttcgaagtca
    19501 tccaggtcca cgggttcctt tgccttcact ttttcaagat tttccatgct cccatatctc
    19561 gcttccgtcc taaaaatccg agcggtcaat tcgttcccgc atctcgagca gcgtgcggtg
    19621 gtacaggctc ttaatcgcgc tctcactctt gtttaacacc gcagcaatct cagagtttga
    19681 tagtttttcg acgaatttca gcaaaatcag ctctcttttt tgcggagaga ggtcgttaat
    19741 tatcctccag agcgcttccg catcctgctt gcgccctatg gaagcgtcca tcgcgggcag
    19801 cctgtcagga atattgacca ccgccatatc ctcgatgggg atttcacgcc ggcgggcttg
    19861 gtcgcggtgg tggttggcga tcaggttgtg cgcgatgcgg tacagccagg cggagaaggg
    19921 cagacccaca aagtggtagc ggttgaggtt attcagggcc ttcaggaaaa cgcgggcggt
    19981 caggtcttcc gcgtcgtgca cgctgccaac gcgaaaataa acatagttat agatccggtc
    20041 gatgtacttc tcgtacagtt ctccgaacgc ttccgggtcg gttttggccc tttccaccag
    20101 ttcagcttcc gcgcgcagtg ggtccacaga atggcattat agcattgccc atccctgcgg
    20161 caaaccgctg gcaatactgc ctggaatccg catctggcag gcgcaagata gggtatactt
    20221 acagtaatgg tacactccgg atggatggtc aaactgcaat tcgggccagc tttgggtgcg
    20281 cgttttttca gggtcccgca atggattgag ttaaccatac caggccagac aagtagaact
    20341 cagacgcccg ttaagggctt attttatgcc aaggatgatc tcccatgaac ggtttcctca
    20401 tcgaaaaaca cctatctgac cttgaccttg acctggacaa actgaccgcg ctggaaggcg
    20461 aacggcaagc ccgccgcctg attatgatcc ccagcgaaag cagcgcccct ctggcggtgc
    20521 gggaactgct ggcatccccc tataccaaca tttacgccga gggctacccg cgcgcggaaa
    20581 cacgcacgat gagtcaggcg gaaattctgg attatgccag ccaactcggc acctaccggc
    20641 gctacagcga cctgcgctac tacaagggcg tggagtacgt ggatatcgtg gaggcgcttg
    20701 cccgccggcg cgccgccgag ctctttgccg ccaactccct ggaagcggac gatttgtggg
    20761 tgaatgtgca gccgctttcc ggcgcgcccg ccaacaatgc cgtctatacc gcgctgctca
    20821 aaccgggcga cacgatcatg ggcatggatt tgctgcacgg cggccacctg acgcacggct
    20881 caccggtgaa ccgttccggc ttgttttaca acgcggtgca ctacacggtg aacccgcaga
    20941 cggagcgtct ggattacgac atgattgcca gcctggcgcg cgaaaccaaa ccaaaaatca
    21001 tcatagcggg ttactcgtcc tacccctgga ttccggattg ggaagcgttc aagcaaatcg
    21061 cagagagcgt cggcgcgtac ctgctggcag atgtctccca tatttcgggg ctgattgccg
    21121 cgaaagtgtg ctcctcgccg gtcggcattg ccgatgtggt aacctttacc actcataaaa
    21181 cgctgtgcgg tccgcgcggc gcggtgatca tcacgcacga caaagagttg ggcgagaagt
    21241 tcgacaaagg cgtcttcccc ggcgagcagg gcggcccgca catcaacacc atcgcagcca
    21301 tggcgctcac ctttggactg gctcgcagcg atgaatttct gcagctgcag cagcaaaccc
    21361 tcaggaacgc cagcgccctg gctgctgcct tccagaaaac cggcgtgaaa gtgccttaca
    21421 aaggcacgga cagccacctg accctgattg actgcaagtc cttccgcgga ccggaaggcg
    21481 tgccgctgaa cggcgacctg gctgcccgta tcctcgacct ggtcggcatc gtcgtgaacc
    21541 gcaacaccat cccgggcgac cgctccgccc tgtctgccag cggcgtacgc ttcggcacca
    21601 cctggctaac ccagcgcggc ttcatggaat ctgactttga aacgatcgcg gaacagatta
    21661 ccaaactctt ccgcgccacc tccccctacc ttatgtgcgc tcaaaacggc aagaagaccc
    21721 gcgccaaggt
//
LOCUS       DAOQAD010000023        21475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_2507_length_21475_cov_5.692437, whole genome shotgun sequence.
ACCESSION   DAOQAD010000023 DAOQAD010000000
VERSION     DAOQAD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 21475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21475
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2507_length_21475_cov_5.692437"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PKL60_03315"
     CDS             <1..1035
                     /locus_tag="PKL60_03315"
                     /inference="COORDINATES: protein motif:HMM:NF041539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor R domain-containing protein"
                     /protein_id="HNW95197.1"
                     /translation="ATRLSLGTTAKDGPEKRKEGTDSQIRLECLGWYARLAWRVYQPP
                     TGLLGNASAQQGSQGLGNTSSFQRIAQSFTTDSGAARPAQVQVRVRRYGSPNDSLRAA
                     LQADNGSGSPSGLDLASATISGAALAAEAYPWTAFSFALPPQLLANSSYWLVLSRTGA
                     VSAANYYLLAVDEKLSYPAGTFKTYNGSAWAARSVPADLVFTLSAFWESTDLILEAAG
                     LLLHDPFKRVSVESESGVYLTPASESPQPADRFVQALLELGTERFTALTCTVEPDLGL
                     VFAERPPAQEAETWRLGPGTGLQKPGGLPCLPGELPVGKWLRCAENPAVYVTGGQISA
                     PDWTPRLESA"
     gene            1117..2403
                     /locus_tag="PKL60_03320"
     CDS             1117..2403
                     /locus_tag="PKL60_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNW95198.1"
                     /translation="MPKATFNFPAGFLWGSAAAAACVEGNPPANDWSEWEQQPGRVLD
                     GSSSAQACEWWSGRWREDFDRAQETNQKILRFSMDWARVQPEPERWDEPALDRYREML
                     LGLKQRGMTPLLSLHHSSNPRWFAEMGGWENEQSAEYYAEYARRVAEALKAHNALWLT
                     FSAPNAYILKAYRAGTFPPGKQDIASAGRALVNMARAHAAAYEAVHALQADAQVGFTH
                     LWPSAGGRADADRNAFPLAFQSGQLEFGGFKQALPQLKGTLDFLGIDYFPLGAGSAPK
                     ALKQTKRKAPAPSASEPASESARAVENAQGLREAVKWGWKFELPMYISANGCDDARDD
                     FRRQYLAAQVHALWHMVNFNAPVRGYLHWSLTDHFAWEQGWGAKYGLWALDPQTQQRT
                     RRQSADFYAEICRTGSLSSEMVEKYCPEVFEQIFPV"
     gene            2527..3369
                     /locus_tag="PKL60_03325"
     CDS             2527..3369
                     /locus_tag="PKL60_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNW95199.1"
                     /translation="MIAYLSKVLPQFILPAGLAAILLFLTLFLIKKKPKTAIWLVLIS
                     LLLVSTAGNAYFSAFLTRSLEWRYMPLTGEVKADAIVLLGGGTEAPDTPRQMVEINSA
                     GDRVLYAAQLYKAGVAPLIILSGGNMSYSQARSTTPAEEMQAMLVGLGVPQEALVLQK
                     QSQNTAEDAYFTSAILAEKQIRTVILVTSAAHMERALMMFASPEISIIPAPTDYSVTQ
                     RSWNNLMRWDWKTILTHIMPAGGAIAQTDLILHEYLGIFFYRVKFLWESLGMQLPSLK
                     GLLR"
     gene            3499..4824
                     /locus_tag="PKL60_03330"
     CDS             3499..4824
                     /locus_tag="PKL60_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNW95200.1"
                     /translation="MKILRSLLGIALVAAVLLSACAPLAETPEQPAPTEPIEKPEEPE
                     QGFSEYTGKDAYIIDPQVDREAVQAAAAASDAFALALYRQLAENDGNLFYSPYSIYTA
                     LMMTYAGAAGNTAEQMKQALAMLLPEGQEHAALNGLNQTLVQNSMFDGEPIFSFHVAN
                     QLWGQKDFPFNEPFLNTLSANYDANLKTVDFSDSESARALINFWVAAQTNDKIKDIIP
                     TGVLNELTRLVLTNAVYFKAAWMNQFDPTKTAQGTFTLADGSTAEVPMMHAQRSMQAF
                     MDEKLAAVELPYVGGMFSMVLVMPAQGSLAEFEASLNGDRLNEILGSLSTASVNLSMP
                     KFKLESSFGLGEAMKNLGMTDAFTPGAADFSGMESTRSLYISDLLHKAYVDVNEEGTE
                     AAAATTVVVGMTSMPAESYEISFDHPFLFFIRDIQTNTILFMGRLADPR"
     gene            complement(4895..5743)
                     /locus_tag="PKL60_03335"
     CDS             complement(4895..5743)
                     /locus_tag="PKL60_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNW95201.1"
                     /translation="MEKVIIATDSSAYLPQEYADKYSIPIISLTLSWDGQSFRDGVDI
                     KATEFYTRMLKSKTLPTTSQVTVQQFVDFFTPMLEAGQKVLYAGISEGISASYNSAIL
                     AKNELGNPKDLEIVETGLVSSALSFLVLSLARASEEGASLEELVEQVPVIQPKIGVYF
                     TVDTLEYLHRGGRINTAARFMGTTLNLKPLLEIRGGKIEAVENVISRKKAITRMLELV
                     KKRVGDATPVRISPFHALCEDECLAMEARAIELLHPVEVIRSEVSPVIGTHVGPGTLA
                     LAWMAG"
     gene            6055..7764
                     /locus_tag="PKL60_03340"
     CDS             6055..7764
                     /locus_tag="PKL60_03340"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNW95202.1"
                     /translation="MQSSLTDNKESLGNEASGPAPEAKQPANADAGKESPVILRKPGW
                     ILLLVFCLALLVDRILFYREPGAQWAIFVNGLLAVVFIAAALEHKRIPLTSVLLALPV
                     SAAAALTMFRSETFTLMALVLFSILGTILLAVSLLNGQGFAYRLREYAVQGLRLVGSV
                     FVGLPAGLMQTAQRTRAGDGADSGSGKQRSGAVLRGILIALPLVALLGLLLASADAVF
                     SSRISALLDWVKIEKPEEFIFRLLFVLGLSYALFGLVWHALTKTGEKLALEPDQPLVK
                     PFLGMTESAIILTALNLLFAAFVLVQVKYFFAGEQNINLEGFTYAEYARRGFFELVVA
                     AIITLLVHYGLASLTRRESRGRKLLFSILASLLLMQTGVILLSAFQRLSLYEAAYGFT
                     QARLTAHVFMVFLGLVLALSIVMEITRTYKRLAVSLLLVLLAFGLTLAFLNVDRTIAR
                     QNVQHAQAGNELDLAYLAGGGLSEDAAPELFAALDDPTLSPQLKEALGLALSCRAARF
                     EQDRADATFWASWHASRSAASKLYRDRADDLAEYPLQNFTEGKGFLVEGSPFLCIYSE
                     TMP"
     gene            7878..8819
                     /locus_tag="PKL60_03345"
     CDS             7878..8819
                     /locus_tag="PKL60_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95203.1"
                     /translation="MKKSKSTWILASIALLVLCAAACKLPFSSTAVPDDAATAALQLA
                     QTQVGISATQTALAPAPTEAPAPAATEPALPPTLEPDTPAPGTTRYTFGNFQFDMPDY
                     LALDVNHTIVPAALEGDEAFPGAIQPEYLSITFDGYIIPDAFHTPEIRVYPVADYMEI
                     SQPATDTFEELNYLLVNRPQTIPYDAGLPFIPFWNAGQIFNAQAKFVDFKSGSGIRFL
                     SMYAQAVYPVDNYNIFFTYQGLSADHAYFISMVLPINSAALPMHAEDPADYEAFINSF
                     STYLQETSAMLNAEAPERFTPTLTVLDAMIASMRIIP"
     gene            8939..>10986
                     /locus_tag="PKL60_03350"
     CDS             8939..>10986
                     /locus_tag="PKL60_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNW95204.1"
                     /translation="MFIPTTPEELKTLNWTELDVILVSGDTYIDSPFSGTALIGQQLI
                     RSGYRVGIIAQPDVKSAADITRLGEPRLFWGVSAGAVDSLVANTTALGKPRRTDDHTP
                     GGLNNRRPDRATIVYANLIRQHFKNTVPIVLGGVEASLRRVAHYDFWSDALRRSVLLD
                     AKADYLLYGMADLSVLALAEALRDGLDPRGLRGLVYASAEMPADYLELPSFEEVCADK
                     DRFIEMFHAFYVNNDPISARGLAQRYGDRYVVQNPPAPTLTTEEMDAVYALPFEHAVH
                     PYYAAQGEVRALETIRFSIPTHRGCYGECNFCAIAVHEGRRVAWRSKASILAEARAMK
                     DRPGFHGIIHDLSGPTANMYGFECRVKAHRGACADKSCIYPEVCPLLGLTHAPHTGLL
                     KAVRQVEGIHKVFVGSGIRHDLVMADEEHGASYMEELVGHHVSGQLKLAPEHSQPSVL
                     KYMRKPGTDSLLTFKQRFDAISERVGKEQYLTYYIIAAHPGCTDSDMVALRKFASEKL
                     GVLPEQVQIFTPTPSTYSSVMYYTEKDPFTGAPLFVEKSQTGKMRQKAAITGWGKKGR
                     AAEEAEKPAPRTNARPESGQVEKPSPVRPGPDTQRVPFVREEPSNSSREDAPVVRVVR
                     DDFGSFLHNQAPEGWVDRRAERAEPERSEWKPRQSDSGRPAREGGERPYRERKDWKPR
                     "
     assembly_gap    10987..11086
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11087..11452
                     /locus_tag="PKL60_03355"
     CDS             <11087..11452
                     /locus_tag="PKL60_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95205.1"
                     /translation="WKPRESSSATSKPREGGERPYRERKDWKSREGSADTRTQREGGE
                     KPYRERKDWKPRESSSATSKPREGGYKRGGKTGSSKTGNRNQGGRDYRVRDQRKDQRN
                     PKKDKPAGEQGNSSSGEHG"
     gene            11449..12645
                     /locus_tag="PKL60_03360"
     CDS             11449..12645
                     /locus_tag="PKL60_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95206.1"
                     /translation="MKTELLLNLQLILGTALLILLGVFTFVLINRIRQRRTQLVSSES
                     SPPAFPYPSERAAEQGAGLAVQMQVEADALIPAEHARWLNSFFLPDPEPGRAFVRTGV
                     AKHWLRYQLTATSLAQAAGLLVCALQAGADPLAQTRFDALLAFCLSRPAANNPDLMSW
                     QVMPDVVPGARLDADPQAEAWLAFALLCGAAQWARSSRFDYTQLARLRLDALLARLQG
                     GTGDMPQSKLISPGFARLFQRTSGNADWLKAANLDALPAQETADPVATGGAQTAVADE
                     EARLALLALAYGLLPEARKHGTPADAFSAGIDLLARLEKLKEETPAVSAPSEDFSPLS
                     LLACYAAAAGSAGSADLAREYWTALSQTEANFGDGLGSTLRLLALMALNGSVWFPEAF
                     REPASA"
     gene            complement(12768..13220)
                     /locus_tag="PKL60_03365"
     CDS             complement(12768..13220)
                     /locus_tag="PKL60_03365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNW95207.1"
                     /translation="MAETAIYTTDVSWKGGHMGHLSLGNGPEMDFSAPPDAQGRAGVL
                     TPEDAFVAAANTCVMLMFIWAAERFKLKLLSYDCRVEGTKRVEIDRTEMFTHLRYWPL
                     IRISAAGELHEIVRARAERALNAAQKYSLVANSVKSEIIIEPVIEILE"
     gene            complement(13213..13971)
                     /locus_tag="PKL60_03370"
     CDS             complement(13213..13971)
                     /locus_tag="PKL60_03370"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNW95208.1"
                     /translation="MPELKQNKVGIVSCSGEELAEGTVSRLAALKVLEELRPGETVTI
                     CLPLFLAGGEGDRAFARFYPTIAIDGCEKRCAFRATEMFSNTPVMGIVISGLTREQGL
                     ERPQGCRKLDETGKKLVEIASIQAAEKVDELLGRRWSRREGVLEPREEDSAQSPKSAP
                     GTEPGQTTCSCGSGIPVLKLKVNAREVEFLALPSIFQNFLENGQPPSPQTLSALMESV
                     KLYTPISSDIEEAARAAVAKAYKAYWDTWQERRG"
     gene            complement(13958..14383)
                     /locus_tag="PKL60_03375"
     CDS             complement(13958..14383)
                     /locus_tag="PKL60_03375"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNW95209.1"
                     /translation="MPAKKKLILLPCSGVGKPFGTITREAAYQVAQEDRPECTQIVPL
                     SLLVMGDADAKALLMDAEIISLDGCALACASKMAAESGAQVCAAFNAMDVYRAKRQYK
                     PQGISSLNEAGQQLARLLAEQVDAAVDKLTEEGTQDARA"
     gene            complement(14385..14828)
                     /locus_tag="PKL60_03380"
     CDS             complement(14385..14828)
                     /locus_tag="PKL60_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HNW95210.1"
                     /translation="MPEVFVFTIDKFSFRVPTDRLYSAEGLWALAKDGIIRTGLSDYL
                     QQRSGDVAFVDVQPGGKRVRRGELLVSIETIKITSDLHSPLAGRIASVNPAMQRSPED
                     INRDPYGEGWVCELEPEDWEEARLSLMTPQAYFAYIQQDAAQELE"
     gene            complement(14913..15152)
                     /locus_tag="PKL60_03385"
     CDS             complement(14913..15152)
                     /locus_tag="PKL60_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNW95211.1"
                     /translation="MLKIKILGPGCRNCQNLEALARRVAQENDIEAEFEHVTERAEIL
                     SYHILATPGLVVNGKVVSAGRIPSDEEVASWLKAA"
     gene            complement(15139..15555)
                     /locus_tag="PKL60_03390"
     CDS             complement(15139..15555)
                     /locus_tag="PKL60_03390"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNW95212.1"
                     /translation="MNTIEKQERTLREYARQAELLKALGHPARLAILDLLRDGPHCVC
                     HLEAHLGLRQAYISQQLAVLRAAGLVDINREGWNVYYAISEPAVFALLDAAQGRVGGA
                     NMVEPGTGINCPCPKCSSEHRQTLQAIPVKEKNAEN"
     gene            complement(15730..15900)
                     /locus_tag="PKL60_03395"
     CDS             complement(15730..15900)
                     /locus_tag="PKL60_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95213.1"
                     /translation="MVGILIIGVLNNAMQLANFPDFSQTVVKGAVLLIAVAFDVYQKN
                     RKAKVAVASSAS"
     gene            complement(16060..16998)
                     /locus_tag="PKL60_03400"
     CDS             complement(16060..16998)
                     /locus_tag="PKL60_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HNW95214.1"
                     /translation="MIITLTPNPSVDRTLKIARLRFNENLRTHDARVDWGGKGFNVSR
                     ALRILDQETLALAWVGGGTGKMLEDGLQDLGIRTDFVWVSEDTRTNTLAQEDAGEWYI
                     RLIEPGPHIPEDAIQALLEKADAYANANDIWVASGSLPQDVPEDFYARLIRLLKAKGV
                     RMFFDTNDEPLRIGLSEAPFLVKPDLAEVASITGIDVTNYEEAKRAALPFLRMGVQHV
                     AITIGNLGVLLASQNLILLASPPKMAARNVTGAGDAVMAGLAYGFSQNMPLNETARWA
                     AAFGAVVISKELLASVSLEELREMLPRVEVRTVNVM"
     gene            complement(17077..18987)
                     /locus_tag="PKL60_03405"
     CDS             complement(17077..18987)
                     /locus_tag="PKL60_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95215.1"
                     /translation="MNSNPQSENKPTSESPTNQAPIRRTPQPSIRRGSGPGALMPGEK
                     ARNFKGSMKQLLSYLGKYKWTILLVMVITSVSVVFTIFGPKILGQATTALYEGVTRMI
                     AGDPRGIDFAEISQFLLRVLALYLGSAMLDLVQGFLMTSISADITYRFRREIAEKINR
                     LPLSFFDHITHGEVLSRVTNDVDTISQTLNQSLSQMITSLVSVVGILIMMLTINIQMT
                     LIALTIVPLSLVLIRNVVKHSQGYFRAQQEYLGHINGHIEEMYGGHIVIKAFNGEDES
                     IAKMKDLNETLYDSAWKAQLHSAFIMPTMRFIGNLGYVAVSIVGGYLVIRKELTLGNI
                     QAFIQYMRSFQQPLMNIANISNVLQQTAAAAERVFEFLNEAEEIPDPAEPVLLGEVKG
                     EVEFRNVHFGYEPNQIIIKDFSVKVEPGQQIAIVGPTGAGKTTLVKLLMRFYELNSGQ
                     ILVDGVDITRLKRADLRDIFGMVLQDTWLYNGTICENIRYGRQDASDEEVVHAADTAY
                     ADHFIRTLPGGYNMLLNEDATNISSGQKQLLTIARAVLSDPRILILDEATSSVDTRTE
                     VLIQKAMNKLMQGRTSFIIAHRLSTIRNADRILVIRDGDIVEQGKHEELLAQNGFYAE
                     LYNSQFALQTNY"
     gene            complement(18984..21227)
                     /locus_tag="PKL60_03410"
     CDS             complement(18984..21227)
                     /locus_tag="PKL60_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95216.1"
                     /translation="MKRLLPYIKPYWLLIVLALGLLYVMASTNLALPDYLSQIINIGI
                     QQNGVDTTIPETIRASSLDKIALFQSEQERAETLAAYERIEPGTSESAALVPYYPGAS
                     LQPVYKLKPLDASATASLEKALTKPLVILYGFQMIEQNPEQASSLVGPELAEKLKSLP
                     AGTDPLSLFTQLPEAQVEQIKAALMQHLDSLEGTILRQMAVRAVGNEYSTLGVNLQKN
                     QTNYILRIGGRMILMALLAVVTSIMVSYLASRTSAGVARDIRAAVFEKVSNFSSAEFD
                     NFSASTLITRTTNDVSQVQQVIFMVIRMAFIAPIMGIGGVIRAIDKSPNMWWLIALAV
                     GILIMFIMLAFTLALPKFKIMQKLVDRMNLVIRENLSGLMVVRAFNKQDFEEQRFDKA
                     NQELTSTTRFVGRVLTAMFPLISLIMTGLSVAIIWVGAREVAASTLQVGNLIAFMQYA
                     TQIMFSFLTLSMLFIFIPRAAISGDRIADVLETPIFIQDPAEPKKLPEPVQGKVEFLN
                     VDFRYPDAEVDVLHDISFTALPGQVTSLIGSTGSGKSTVVNLIPRFFEVSKGSIQIDG
                     VDIRSLNQHDLRERIGFVPQKGLLFSGTIDSNMRIGKPNVDPEAVREAIETAQAADFV
                     FGKPEGVDAEVAQGGTNFSGGQKQRLSIARALVKKPPIYIFDDSFSALDFKTDATLRQ
                     ALKKKVGNSTVLIVTQRVATAKNSDQILVLDNGRLIGQGTHQELLKTCQTYQEIASSQ
                     LSKEELA"
     gene            complement(21250..>21475)
                     /locus_tag="PKL60_03415"
     CDS             complement(21250..>21475)
                     /locus_tag="PKL60_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95217.1"
                     /translation="GLVNRQVDPADRRGKRLCLTNLGQEKVAPASEARRAWLHDLIDG
                     FTPEQQAALQPAFKILLEGMNKVFTQTNNS"
BASE COUNT         4366 a         6126 c         6276 g         4607 t
ORIGIN      
        1 gccacgcgat tgagcttggg tacgaccgcg aaggacggcc ccgagaagcg taaggaaggg
       61 acagactctc aaatccgcct ggagtgcctg ggttggtatg cgcgcctggc ctggcgtgtg
      121 taccagccgc cgaccggcct gctgggcaac gcttctgcgc agcagggcag ccagggcctg
      181 gggaatacga gctcttttca acggattgcg cagtcattca cgacggacag cggcgccgcc
      241 cgacctgccc aggtgcaggt gcgcgtccgg cgctacggca gcccgaacga ctcgctccgg
      301 gcagcgctgc aagcggataa cggttccggc agcccgagcg gcctggattt ggcttccgcg
      361 acgatttcgg gcgcggcgct ggcggcggaa gcgtacccgt ggacagcctt ttcattcgcg
      421 ctaccgccgc agctgctggc aaattccagc tattggctgg tgctctcccg aacgggggcg
      481 gtcagcgcag caaattatta cctgctggcg gtggatgaga agctttctta tcccgctggc
      541 acgttcaaga cctacaacgg cagcgcctgg gcagcgcgct cggtgcccgc ggacctggtg
      601 tttacgctca gcgcattctg ggagagcacc gatttaattt tggaagccgc cgggctctta
      661 cttcacgacc ctttcaaaag ggtgagcgtg gaaagcgaga gcggcgtgta cctgacccca
      721 gcatctgaga gtccgcagcc ggcggaccgc tttgtgcagg cgctgctaga gcttggcaca
      781 gagcgtttca ccgcgctgac ttgcacagtg gagccggatt tagggttggt gtttgcagaa
      841 cgcccgccag cgcaggaagc ggagacctgg cgcctgggcc ccgggacggg tttacaaaaa
      901 ccgggcgggc tgccctgcct gccgggggag ctgccggtgg gaaagtggct gcgctgcgct
      961 gagaaccccg cggtctatgt gaccggcggc caaatcagcg ccccggattg gaccccgcgc
     1021 ctggaaagcg cctgaaaacc cccgcgcata gaaaggaaac gcccgcccgg agttaaagcg
     1081 gttaaaatga gggcattaag taagatggag aaacttatgc caaaagcaac ctttaacttt
     1141 ccggcgggct ttttgtgggg cagcgcggcg gcggctgcct gcgtggaggg caatccccct
     1201 gccaatgact ggtccgaatg ggaacagcag cccggccggg ttttggatgg aagcagctct
     1261 gcgcaggctt gcgaatggtg gtccgggcgt tggcgcgaag actttgaccg cgcgcaggaa
     1321 acgaatcaaa aaatcctgcg tttttcgatg gattgggcgc gcgtgcagcc cgagccggag
     1381 cgctgggatg agccggcgtt ggaccgttat cgcgagatgc tgctcggtct gaagcagcgc
     1441 gggatgacgc ccttgctcag cctgcaccat tccagcaacc cgcgctggtt tgcggaaatg
     1501 ggcggctggg agaatgagca gtctgcggaa tactatgcgg agtacgcgcg gcgggtggca
     1561 gaagccctga aggcgcacaa tgcgctgtgg ctgactttca gcgcgccaaa tgcttacatc
     1621 cttaaggctt accgcgcagg gacatttccg cccggcaagc aggatatcgc ttccgccggc
     1681 cgggcgctgg tgaacatggc cagagctcat gcggcggcct acgaagccgt gcatgccttg
     1741 caagccgacg cgcaggtcgg cttcacgcat ctctggcctt ccgctggcgg gcgcgcggat
     1801 gcggaccgta atgcctttcc gctggcattc cagagcggcc agctggaatt tggcggattt
     1861 aagcaggcgc tgccccagct aaaaggtacc ctggactttc tcgggattga ttatttcccg
     1921 ctcggcgcgg gcagcgctcc caaggcgcta aagcaaacca agcgaaaagc gcccgcccca
     1981 agcgcgtcag agccggcctc ggaaagcgcg cgcgcggtgg aaaacgcgca aggcttgcgc
     2041 gaagcggtca aatggggctg gaagttcgag ctgcccatgt acatcagcgc gaacggatgc
     2101 gatgatgccc gcgacgattt caggcgtcag tacctggccg cgcaggtgca tgctctctgg
     2161 cacatggtga actttaacgc tcccgtgcgc ggttaccttc actggagcct gacagaccac
     2221 tttgcctggg aacagggctg gggcgcgaaa tacgggctgt gggcgctgga cccccagaca
     2281 cagcagcgca cgcgccggca aagcgccgat ttctatgccg aaatctgccg gacgggcagc
     2341 ctttcttcag agatggtcga gaagtactgc ccggaagtct tcgagcagat tttcccggtg
     2401 tgagctgcct ggaaagcgga tgaaacaggc gcgcggcagc attttgggat tcttttgctg
     2461 cttttctcta aaaacgggcg tgtttcttgc aacatattct gcgaatgccc aattaaggag
     2521 ctggatatga ttgcttatct ttcaaaagta ctgccgcagt ttattttacc cgccggcctg
     2581 gccgcgattt tgctgttcct gacactcttt ctgataaaga agaagccgaa aactgccatt
     2641 tggttggtgc tcatcagcct gcttctggtc agcacggccg gcaacgcgta tttctccgcg
     2701 ttcctcaccc gctctctgga gtggcgctac atgccactga ctggcgaggt gaaggcggat
     2761 gccatcgtgc ttttgggcgg cggcaccgag gcgccggata cgccgcgcca gatggtggaa
     2821 atcaattccg ccggggaccg ggtgctgtat gccgcgcagc tctacaaagc cggcgttgcc
     2881 ccgctcatca tcctttccgg cggcaatatg agctacagcc aggcgcgcag caccacgccc
     2941 gcggaggaaa tgcaggccat gctggtgggg cttggcgtgc ctcaggaggc gctggtgctg
     3001 cagaagcagt ctcagaatac agccgaggac gcctacttta ccagcgcgat tctggctgag
     3061 aagcaaatcc gcaccgtcat tctggtcaca tcagcggcgc acatggaacg cgcgctgatg
     3121 atgtttgctt cgccggaaat cagcataatc cccgcgccca ccgattacag cgtgacccag
     3181 cgttcgtgga ataaccttat gcgctgggac tggaaaacca tcctgacgca tatcatgccg
     3241 gctggaggcg ccatcgcgca gaccgattta attctgcacg aatacctggg tatcttcttc
     3301 taccgggtca aattcctatg ggaatctctg ggcatgcagc tgccctcgct taaggggttg
     3361 ctgcgctaag ctaaccttcc tgacctgcgg gaataaagcc cgcacccttg agaacctaag
     3421 ccgcgaggaa caatcgcggc ttttcacgcg tctatagggt ggagccttta ttcacggccc
     3481 ctgaaccagg aggatgacat gaagattttg agaagcttat taggtatcgc gcttgtggca
     3541 gctgtgcttc tgagcgcatg cgcgccgctg gcggagacgc ctgagcagcc cgcccctacc
     3601 gaaccaatcg agaagcctga agaacccgag caaggtttct cggagtatac cggcaaagac
     3661 gcttacatta tcgatccgca ggtagataga gaagctgtac aggctgccgc tgctgccagc
     3721 gatgcttttg ccttggcgct ttaccgccag ttagcagaaa atgacggcaa cttgttctat
     3781 tccccttaca gcatttacac cgcgctaatg atgacctacg ctggcgcggc ggggaatacc
     3841 gcggaacaga tgaagcaggc tttggcgatg cttctccccg aaggtcagga gcacgccgcg
     3901 ctgaacggct tgaaccagac gctcgtgcag aacagtatgt tcgacggcga accgatcttc
     3961 agctttcatg ttgccaacca actctgggga cagaaggact ttcctttcaa tgagccgttc
     4021 ctgaacacgc tttccgccaa ttacgacgcg aatctgaaaa cagtcgattt tagcgattcc
     4081 gaaagcgcac gcgcgctcat caacttttgg gtggccgccc aaaccaacga caaaatcaaa
     4141 gatatcatcc cgacaggtgt gctgaacgag cttacccgcc tggtgctaac caacgcagtg
     4201 tacttcaaag ctgcctggat gaaccagttt gacccaacca agaccgcgca gggcaccttt
     4261 acgctggcgg acggcagcac cgcggaggtc cccatgatgc atgcgcagcg cagcatgcag
     4321 gcctttatgg atgagaaact cgcggccgtg gaactgccct atgttggtgg tatgttttcg
     4381 atggtgctgg tgatgcccgc gcagggcagc ctggcggagt ttgaagcttc cttgaatggg
     4441 gacaggctga atgaaatcct gggatcgctt tctactgctt cggtaaactt gagcatgcca
     4501 aagttcaaac ttgaaagttc ttttggtctc ggcgaagcga tgaaaaatct ggggatgacg
     4561 gacgcgttca cacccggcgc cgcggacttt tccgggatgg aaagcacccg cagcctgtat
     4621 atctcagacc tgctgcacaa agcttacgtg gacgtgaatg aagaaggcac ggaagctgcc
     4681 gcggcgacta ccgttgtggt cgggatgacc tccatgccgg cggaaagcta cgaaatcagc
     4741 tttgaccatc cgttcctgtt ctttatccgg gatatccaga ccaacacgat tctctttatg
     4801 gggcgtctgg ctgacccccg ctgaaactgg cagggcggta aattcaagag ccggtcgggt
     4861 gcgaccggct ctttccgttt ggtttacggc tggcttagcc ggccatccag gccagggcga
     4921 gcgtgccggg gccgacatgc gtgccaatca cggggctgac ttcggagcgg atgacttcca
     4981 ccgggtgcaa aagttcaatc gcccgggctt ccattgccag gcattcgtct tcgcacagcg
     5041 cgtggaaggg gctgatccgc accggcgtgg cgtccccaac gcgttttttc accaactcga
     5101 gcatgcgggt aatcgctttc ttgcgagaga tgacgttttc caccgcctca attttgccgc
     5161 cgcggatttc aagcagcggt ttgaggttca gtgtggtgcc cataaagcgc gcagcggtgt
     5221 taatgcggcc gcctctgtgc aggtattcca gcgtgtcaac ggtaaagtac acgcctattt
     5281 tgggctggat gaccggcacc tgctccacca attcttccag actggcgcct tcttcggaag
     5341 cgcgcgccag gctgagcacc aggaaagaaa gcgcgctgga caccagtccg gtttcaacga
     5401 tttccaagtc tttcgggttg cctaactcat ttttggcgag gatagcggag ttatacgagg
     5461 cggaaatgcc ctccgatatg ccggcataga gcactttttg gccggcctcc agcataggcg
     5521 tgaagaagtc cacgaattgc tggactgtaa cctggctggt ggttggaagg gttttggatt
     5581 tgagcatgcg tgtataaaac tcggtggctt tgatgtcgac gccgtcccgg aagctttgtc
     5641 cgtcccagct taacgtcagc gaaatgatcg gaatgctgta cttatctgcg tattcctggg
     5701 gaagatacgc gcttgaatct gtggcgatga taactttttc cataatttgt cctttcctgg
     5761 atagaatatt ctctagtata gcatgtcttg gccgggcttg cggcgcatag tctgctggca
     5821 agcctgcctg ctgctggagt tcgtgcgtga tttggaaggg cgagcccgca atggacaggc
     5881 aagccctcaa atcagggctc attctttaga aaaccccttg atgcgctgcg gcttctcaac
     5941 catttctgat acactttgag agcaattgaa aacccgtgag agtggatgca ggtctttgga
     6001 ataatgcctt ttttataatc tgtggtaaat ctcacgcaag acaaggagca ctttatgcaa
     6061 tcttctctta cagataacaa ggaatccctc gggaacgaag cttccgggcc cgccccggaa
     6121 gcgaagcagc ccgcaaacgc ggacgcgggc aaggaaagcc cggtcatcct gcgcaaaccc
     6181 ggctggatat tgctgctggt cttttgcctg gcgctgctgg tggaccgcat cctgttttac
     6241 cgcgagccgg gcgcgcagtg ggctatcttt gtaaacggcc tgctggcggt tgtgttcatc
     6301 gccgccgcgc tggagcacaa acgcatccct ctcacaagcg tgctactggc gctgcctgtt
     6361 agcgctgcgg ctgcgctcac catgttccgc tcggaaactt ttaccctgat ggccctggtg
     6421 cttttttcca tcctgggcac gatccttttg gccgtcagcc tgctgaacgg gcagggtttt
     6481 gcctaccgcc tgcgggagta cgccgtgcag ggtctgcggc tggtggggtc ggtattcgtc
     6541 ggtctgcccg cgggtttgat gcagaccgcg cagcgcaccc gggctggtga tggcgcggat
     6601 tcaggcagcg gcaaacagcg ctcaggcgca gtgctgcgag gtatcctcat cgcgctgccc
     6661 ctggttgccc tgctggggct tttgctcgcc tccgccgacg cggttttttc cagccgcatt
     6721 tccgccctgc tggactgggt caagattgag aagcccgaag aatttatttt ccggctcttg
     6781 ttcgtgctgg gcctcagcta cgcgttgttc ggactggtgt ggcacgcgct gacgaagacc
     6841 ggcgaaaagc tggcgctgga accagaccag ccgttggtga aacctttcct ggggatgacc
     6901 gagagcgcaa tcattctgac agccttaaat ctgctcttcg cggcctttgt cctggtacag
     6961 gtcaaatatt tctttgcggg cgagcaaaat atcaacctgg agggcttcac gtacgcggaa
     7021 tatgcccggc gcggcttctt tgagctggtg gttgcggcaa tcatcacgct gctggtgcat
     7081 tatgggctgg cttctctcac gcgccgcgaa agccgcgggc gaaagctgct cttttccatc
     7141 ctcgcaagcc tgctcttgat gcaaaccggt gtcattttgc tttccgcatt tcagcgtctc
     7201 agcttgtacg aggccgctta cggctttaca caggctcgcc tgacggcgca cgtcttcatg
     7261 gtttttctgg gtttggtgct ggcgctttcc attgtgatgg agattacccg cacgtataag
     7321 cgcctggcgg tcagcctgct gctggtcttg ttggcattcg gattgacgct ggctttcctg
     7381 aatgtggacc gcactatcgc caggcagaac gttcagcacg cccaagccgg caacgagctg
     7441 gacctggcct acctggcagg cggcgggctt tcggaagacg ccgctccgga gctctttgcc
     7501 gccctggacg accccacgct gagcccgcag ctgaaagaag cgcttggatt ggcgctttcc
     7561 tgccgggcgg cgcgcttcga gcaggaccgc gcggatgcga ctttctgggc atcgtggcat
     7621 gcttcccggt cggctgccag caagctgtat cgcgaccgcg cggatgacct ggcggagtat
     7681 ccgctgcaga atttcaccga aggcaagggc tttttggtgg agggctcccc gtttctttgt
     7741 atctattcag aaacgatgcc ttaaccaact ggaatgtatt cagcgaaata gcagcgcgga
     7801 aaactccgcg ctgctttatt tgccgttcaa atcctgtata atacttaagt cccattggtt
     7861 tttacattgg aggagagatg aaaaaatcaa aaagcacctg gattttagca agtatcgcgc
     7921 tgctcgttct ttgtgccgct gcctgcaagc ttccattttc gtccactgcc gtgccggatg
     7981 atgctgctac cgccgcccta cagctcgcgc agacccaggt cggaataagc gctacccaga
     8041 ccgcgcttgc tccagctccg acggaggctc cggcgcctgc cgccacggaa cccgccctgc
     8101 ctcctaccct ggagccagac acgcccgccc cgggcaccac ccgctatact ttcggaaatt
     8161 ttcagtttga tatgcccgac tacctggctc tggatgtgaa ccatacaatc gtcccggcgg
     8221 cgctggaggg ggatgaggct ttcccaggag ctatccaacc ggaatatctt agcatcactt
     8281 ttgacggcta catcattccg gatgccttcc acactcccga aatccgggta tatcccgtcg
     8341 cggattatat ggaaatcagc cagccagcga ccgatacgtt tgaagagctc aactatttgt
     8401 tggtgaaccg cccacaaacc atcccatatg atgccggtct gccatttatt ccattctgga
     8461 atgccgggca gatttttaac gcgcaggcga agtttgtgga tttcaagtcc ggatcaggca
     8521 ttcgcttcct ttcgatgtac gctcaagcgg tttacccggt agataattac aacattttct
     8581 ttacctacca ggggctaagc gccgaccatg cctactttat cagcatggtt ttgcccatca
     8641 acagcgccgc gctgcccatg cacgcggaag accccgcgga ttatgaggcg ttcatcaaca
     8701 gcttcagcac ctatctgcag gaaaccagcg cgatgctcaa cgcggaagct cccgaacgat
     8761 tcacgcccac attgaccgtc ctggatgcaa tgattgcctc gatgcggatc attccctgag
     8821 gctgcgggcc cgccgcacct gcctgatcat tcccccactt gcgcagggag ccagccggct
     8881 ccctgcgtcc atctcgacaa ccgcgccagg ctgagcgatt ttcggttatg attacagcat
     8941 gtttatccca accacacccg aagaacttaa gacactgaac tggacggagc tcgacgtcat
     9001 ccttgtcagc ggagatacct atatcgacag tccgttttcc ggcacggcac tgattggtca
     9061 gcagttgata cgcagcggtt atcgcgtcgg gattatcgcc cagccggacg tgaaaagcgc
     9121 ggcggatatt acccgcctgg gagaaccgcg cctgttttgg ggcgtcagcg cgggggcggt
     9181 tgattcgctg gtcgccaata ccacagcgct gggcaaaccc cgccgcacgg acgaccatac
     9241 ccccggcggc ctgaataacc gccgcccgga ccgcgcgacg attgtgtatg ccaatctcat
     9301 ccgccagcat tttaagaaca ctgttccgat tgtgctgggc ggcgtggaag cctctttgcg
     9361 gcgcgtcgcg cactacgatt tttggtctga tgccttgcgc cgttcggtgc tcctggacgc
     9421 caaagccgat tatctgctct acggcatggc ggatctgtct gttttggcct tggcggaagc
     9481 gctccgcgac ggtttagacc cgcgcggctt gcgcgggctg gtgtacgcca gcgcggaaat
     9541 gcccgcggat tacctggagc tgccgtcttt cgaagaggtc tgcgccgata aagaccgctt
     9601 cattgagatg tttcatgctt tttacgtcaa taacgaccct atcagcgcca ggggtctggc
     9661 gcagcgctac ggcgaccgat acgtggtgca aaacccgccc gcgccaaccc tgactaccga
     9721 ggaaatggac gcggtttacg cgctgccttt cgaacacgct gtgcacccgt attacgcggc
     9781 gcagggcgag gtgcgcgcgc ttgaaaccat tcgcttttcc atccccacgc accgcggctg
     9841 ctacggcgag tgcaacttct gcgcgattgc cgtgcacgaa ggccggcgcg tggcctggcg
     9901 atccaaggcg agcatcctgg cggaagcgcg cgcgatgaag gacaggccgg gttttcacgg
     9961 catcatccac gacctttccg gtccgacggc gaacatgtac ggcttcgagt gccgtgtgaa
    10021 agcccaccgc ggcgcatgcg cggacaaaag ctgcatctac cccgaggttt gcccgctgct
    10081 cggtctgacg catgccccgc ataccggttt gctgaaagcc gtgcgccagg tggaagggat
    10141 tcacaaggtg ttcgtcggct ccgggatcag gcacgacctg gtgatggctg acgaggaaca
    10201 cggcgccagc tacatggaag aattggttgg gcaccacgtt tccgggcaac taaagcttgc
    10261 gccggagcat tcgcagccct cggtgttgaa atacatgcgc aaacctggca ctgacagcct
    10321 gttgacgttc aaacagcgtt ttgatgccat cagcgagcgc gtgggcaaag agcaatacct
    10381 gacttactac atcattgccg cgcaccccgg ctgcactgac agcgacatgg tcgctttgcg
    10441 caagtttgcc agcgaaaaac tgggcgtgct gccggaacag gtgcagatct tcaccccgac
    10501 cccgtccact tattccagcg ttatgtatta caccgagaaa gatccgttta caggcgcacc
    10561 tttgtttgtg gagaagagcc agaccggaaa aatgcgccaa aaagccgcta tcaccggttg
    10621 gggcaagaaa ggcagagcag ccgaggaggc ggaaaagccc gcgccgcgca caaatgcgcg
    10681 ccctgagagc gggcaggtgg agaagccgag cccggtcagg cccgggccgg atacgcagcg
    10741 cgtgcctttc gttcgggagg aaccctccaa cagcagccgg gaagacgcgc cggttgtgag
    10801 ggtggttcgc gatgattttg gcagcttcct gcataaccag gccccggaag gctgggtaga
    10861 ccgcagagct gagcgcgctg aacctgagcg ttcggaatgg aagccgcggc aatctgattc
    10921 tggcagacct gcgcgcgagg gcggggagag accctaccgc gagcgcaagg actggaaacc
    10981 gcgcgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgga aaccgcgcga
    11101 gagttcctct gccacaagca aaccgcgcga gggcggggag agaccctacc gcgagcgcaa
    11161 ggactggaaa tcgcgcgaag gctctgctga tacccgaacc cagagagagg gtggggagaa
    11221 accctaccgc gagcgcaagg actggaaacc gcgcgagagt tcctctgcca caagcaaacc
    11281 gcgcgagggc ggctacaaac gcggcggaaa aaccggctct tccaaaacgg gcaatcgcaa
    11341 ccaaggcgga cgggattacc gcgtccggga ccaacgcaaa gaccagcgca acccaaagaa
    11401 ggacaagcca gctggagagc aagggaattc ttcctcaggt gagcacgggt gaaaactgag
    11461 cttttgctga atttgcagct catcttggga accgccctgc tgattctgct gggggtcttc
    11521 acctttgtgt tgatcaaccg catccgccag cgacgcacgc agcttgtcag cagcgagagc
    11581 tctccgccgg cgttcccata cccatccgag cgggctgctg agcagggcgc cgggcttgcc
    11641 gtccaaatgc aggtggaggc agacgcgctc atcccggcgg agcacgcgcg ctggctgaat
    11701 agcttttttc tgcccgatcc tgagcccggg cgcgcgtttg tgcgcacggg cgtcgccaag
    11761 cactggctgc gctaccaact gaccgccacc agcctggctc aggctgcggg cctgctggtt
    11821 tgcgcgctgc aagcgggcgc ggaccccctg gctcaaacgc ggttcgacgc cctgctcgcg
    11881 ttttgcctca gccgccccgc ggccaacaac cctgacctga tgagctggca ggtaatgccg
    11941 gacgtggtac ccggcgcgcg cctggacgcc gacccgcaag ccgaagcctg gctggcattc
    12001 gcgctgcttt gtggggcagc ccaatgggcg cgctcttccc gctttgatta cactcaactg
    12061 gccaggctca ggttggatgc cctgttggcg cggctgcaag gcggaacggg ggatatgccg
    12121 caatcgaagc ttatcagtcc gggtttcgcc cgcttattcc aacgcacgag cggaaacgct
    12181 gattggctca aggctgctaa cttggacgct cttcccgctc aagagaccgc cgacccagtc
    12241 gccactggcg gtgcgcaaac tgctgtggcg gatgaagaag ccaggctggc gctgctggcg
    12301 ttggcgtatg ggctgctgcc cgaagcccga aaacatggta caccggcaga cgctttctca
    12361 gcggggattg atttgctcgc caggctggaa aagctgaaag aagaaacgcc cgcggtgagc
    12421 gcgccgtcgg aagacttcag cccgctgagt ctgttggcct gctatgcggc tgcggctgga
    12481 agcgcgggta gtgcagactt agcgcgggaa tattggacgg cgctcagtca gacagaggca
    12541 aactttggcg acgggcttgg ctcgacgtta cgcttgcttg ccttgatggc gctgaatgga
    12601 agcgtctggt ttccggaagc tttccgcgag cctgcatctg cctgaggctt tcgttatcgg
    12661 gttggcgcga atggtcggct tgccttggag gcataaagcc aattcccgcg caaattcccg
    12721 gaattctgaa gggctgacag gaaaaacaat ccccgccggc cgagctctta ctcgagtatc
    12781 tcgataaccg gctcaatgat aatctctgat tttactgaat tggcgaccag gctgtatttt
    12841 tgagcggcgt tcaaggcacg ttccgcgcgc gcgcgtacga tctcgtgcag ttcgcccgcg
    12901 gcagaaatcc ggataagcgg ccagtaccgc agatgggtaa acatctcggt gcggtcaatt
    12961 tcaacgcgct tggtgccttc cacccggcag tcataggaga gcagcttgag cttgaaacgc
    13021 tcggcagccc aaatgaacat caacatcacg caggtattgg cagccgcgac aaaagcgtct
    13081 tcaggggtga gcacgccggc gcggccttgc gcgtctggcg gcgcggaaaa atccatctca
    13141 gggccgttgc cgagcgaaag atgtcccata tgcccgcctt tccagctgac atctgtggtg
    13201 taaatggcgg tttcagccac ggcgctcctg ccaggtatcc cagtatgcct tgtaggcttt
    13261 cgcgacggct gctcgcgccg cttcttcaat atccgaagat atgggtgtgt agagctttac
    13321 actctccatc agcgcgctaa gcgtttgcgg ggaaggtggt tggccgttct ccagaaagtt
    13381 ttggaaaatc gacggcagcg ccagaaattc cacttcgcgc gcgttaactt tcagcttcag
    13441 gacaggaatc ccggaaccgc aggagcacgt tgtttgcccg ggctcggttc ctggcgcgct
    13501 cttcgggctt tgagcgctgt cttcctcccg cggctcaagc acgccctccc gtcggctcca
    13561 gcgtctgcca agcaattcat ctactttttc agctgcctgg atagaagcga tttcgaccag
    13621 ctttttaccg gtctcatcca acttccggca gccctggggc ctttccaggc cttgctcccg
    13681 ggtgagcccg ctgatgacaa tgcccatcac aggggtgttg gagaacattt ccgttgccct
    13741 gaaggcgcag cgcttttcgc agccgtcaat cgcgatggtc gggtagaacc tggcgaaggc
    13801 gcggtcgcct tcaccgccag ccaaaaagag cggcaagcaa atcgtgacgg tttcgcccgg
    13861 gcgcagctcc tccaatactt ttaaggcagc caggcgggag actgtgcctt ctgccagttc
    13921 ttcgcccgaa caggacacga tgccgacttt gttctgctta agctctggca tcctgcgtcc
    13981 cttcttcggt cagtttatcc accgctgcat ccacttgctc cgccagcagc cgggccaatt
    14041 gttgcccggc ctcgttcagg gaggaaatgc cttgcggttt gtactggcgt ttcgcgcggt
    14101 aaacgtccat cgcgttgaaa gccgcgcaga cttgcgcgcc ggattccgcc gccattttgg
    14161 aagcgcaagc aagcgcgcag ccgtccagac tgatgatttc cgcgtccatc agcagggctt
    14221 tcgcgtcagc gtcccccatg acgagcagcg agagaggcac gatttgggtg cattccggcc
    14281 ggtcttcctg cgctacctga taggcggctt cgcgggtgat ggtcccgaag ggtttgccaa
    14341 cgccgctgca aggcaggaga atgagcttct ttttcgcggg catcttactc caactcctgg
    14401 gctgcatcct gttgaatgta cgcgaagtag gcttggggcg tcatcaggct gaggcgggct
    14461 tcctcccaat cttccggttc cagttcgcag acccagccct caccataagg gtcgcggttg
    14521 atatcctccg ggctccgctg catggcaggg ttaacgctgg caattcgtcc ggccaacggg
    14581 ctgtgcaaat cggatgtgat tttaatggtt tcgatgctga ccaacagctc ccccctccgg
    14641 acccgctttc cgcctggctg cacgtccaca aaggcaacat ccccgctgcg ctgctggagg
    14701 taatccgaga gacctgtgcg gataatccca tccttcgcca acgcccacaa gccttcagcg
    14761 ctgtacaggc ggtccgtggg gacccggaag ctgaatttat ctatcgtaaa cacgaaaacc
    14821 tcaggcatgg tccggagtcc aatctgcgcg taggtataga aagaagaaat gcagcctggg
    14881 cgccggcagc tcggagcaag aatagcggtc catcaggcag cttttaacca ggacgcaact
    14941 tcttcatccg aggggatgcg gccagcggaa acgactttgc cgttcacgac cagaccgggc
    15001 gtcgcgagga tatggtaaga caagatctcc gcccgctcgg tgacgtgttc gaactcagcc
    15061 tcgatatcgt tttcctgcgc cacacggcgc gcgagggctt caaggttttg acaattgcgg
    15121 cagccgggtc caagaatctt aattttcagc atttttctcc ttcacaggta tagcttgcag
    15181 ggtttggcgg tgttcactgg agcactttgg gcaggggcag ttgatcccgg tgccgggttc
    15241 caccatattg gcgcctccaa cccgcccttg cgctgcatcc agaagcgcga aaaccgcggg
    15301 ctctgaaatg gcataataga cattccaacc ctcccggttg atatccacca gccccgccgc
    15361 gcgcagcaca gccagctgct gggaaatata agcctggcgc aaacccaggt gcgcttcgag
    15421 atggcagacg cagtgcgggc catcgcgcag taggtcgagg atcgccaggc gcgcgggatg
    15481 accaagcgct ttcagcagct cggcttgtcg cgcgtattcc ctgagagtcc tttcttgttt
    15541 ttcaatcgta ttcataataa tgaatataat tatcgccttt ccgatgattc tgtcaagggc
    15601 gaaatatgga tacttttcct gcggatgggc agcctcagcc cccagtgcta tgtcctagaa
    15661 aggcaaaaaa ggaccccaca atcgtgaggt cccatatatt agccgcgcga aagctgcaat
    15721 tcgggcgatt tatgaagctg aactcgcgac agcaactttg gctttgcgat ttttctgata
    15781 gacatcaaag gctaccgcaa tcagaagcac ggcacccttg accaccgtct gggaaaagtc
    15841 cggaaagtta gccagctgca tggcgttgtt cagcacgccg ataatcaaga tgccgaccat
    15901 caatccgaag atggtaccct cgccgccctt aaagctgacg ccgcccagca gcgccgccga
    15961 aaggcagcgg ggctgatggc taaactatca gcggtatggc cagaccatca gcgttataca
    16021 attctgtttt ggattgaccg ggagtggaaa tagtgccgct tacatcacat taaccgtgcg
    16081 cacttccacg cgcggcagca tctcgcgcaa ctcctcaagc gaaaccgaag ccagcagctc
    16141 tttcgatatg actaccgcgc caaaggcggc agcccagcgc gccgtttcat tgagcggcat
    16201 attctgcgaa aaaccgtaag ccaggcccgc catcaccgcg tctcccgcgc cggtcacgtt
    16261 gcgcgccgcc atcttgggcg gggaggccag caatatcagg ttttgggagg ctaacaaaac
    16321 tcccaggttc ccgattgtga tggccacgtg ctgcacgccc atgcgcagga agggcaatgc
    16381 ggcgcgtttg gcctcctcat aatttgtcac gtcaattccg gtgatgctgg cgacttccgc
    16441 caggtcgggt ttgaccagaa aaggcgcctc gctcaagccg attctgagcg gctcgtcgtt
    16501 cgtgtcgaag aacatgcgca cgcctttggc tttcaacaaa cgaatcaggc gggcgtaaaa
    16561 atcctccgga acgtcttgcg gcaggctgcc cgaggcgacc caaatgtcgt tcgcgtttgc
    16621 gtacgcgtcc gctttctcga gcagcgcctg gatggcatcc tccgggatgt gcggccccgg
    16681 ctcaatcagg cgaatatacc attcccccgc gtcttcctgc gccagtgtgt tggtgcgagt
    16741 gtcttccgac acccatacga aatctgtgcg gatgcccaaa tcctgcagtc cgtcttccag
    16801 cattttgccc gtgccgccgc cgacccatgc cagggcgagc gtttcctggt ccagaatgcg
    16861 cagcgcgcgg gagacgttga aaccttttcc gccccagtcc acacgcgcgt cgtgtgtgcg
    16921 caggttttcg ttgaagcgca gtcgggcgat tttgagggtg cgatcaacac ttgggttagg
    16981 agtaagcgtg atgatcatgc gctaattcta cttgaaaaaa gcggggtctg ccgttttcgc
    17041 gcaaaccccg ctttcgagag caaagtatta ttcggctcag tagttcgttt gcagggcgaa
    17101 ctggctgttg tacagctcgg catagaagcc gttttgcgcc agtaattcct cgtgtttgcc
    17161 ttgctcgacg atgtcgccat ccctgatcac cagaattcgg tccgcgttgc gaatggtgga
    17221 gagccggtgc gcgatgatga agctggtgcg cccctgcatg agtttgttca tcgctttttg
    17281 aatcaagacc tccgtacggg tgtccaccga gctggtggct tcgtccagaa tgagaatcct
    17341 ggggtcggag agcacagcgc gggcgatggt aagcagctgt ttctgtcctg aggagatatt
    17401 ggtggcgtcc tcgttcagca gcatgttata cccaccgggc agcgtgcgga tgaagtggtc
    17461 ggcgtacgcg gtatccgcgg catgcaccac ctcctcatcg ctggcgtcct gtctgccgta
    17521 gcgaatgttc tcgcagattg tgccgttgta cagccaggta tcctgcagca ccatgccaaa
    17581 gatatcgcgc aggtcagcgc gtttcagtcg ggtgatatcc acgccgtcga cgaggatttg
    17641 accgctgttc agttcgtaga agcgcatcag cagcttcacc agcgtggttt tccccgcgcc
    17701 ggtggggccg acgatggcga tttgctggcc gggttccacc ttgaccgaga agtctttgat
    17761 gataatctgg ttcggctcat agccaaaatg tacattcctg aattccacct cgccttttac
    17821 ttcccctaat agaacaggct cagcggggtc gggaatttcc tccgcttcgt tcaggaattc
    17881 aaacacgcgt tctgccgccg cggcggtttg ctgcaggacg ttggagatgt tggcgatatt
    17941 catcagcggc tgctggaaac tgcgcatgta ctggatgaaa gcctgaatgt tgccgagagt
    18001 caattcctta cgaatgacca gatagccgcc cacgatggag accgccacgt aacccaggtt
    18061 gccgatgaag cgcatcgtcg gcatgatgaa ggctgaatgc agctgggctt tccaggcgct
    18121 gtcgtaaagc gtctcgttca ggtctttcat tttggcgatg ctttcatcct cgccattaaa
    18181 ggcttttatg acgatatgcc cgccgtacat ttcctcgata tgtccgttga tgtggccgag
    18241 gtattcctgt tgggcgcgga aatagccctg ggaatgcttg accacattgc ggatcagcac
    18301 cagcgaaagc ggcacaatgg taagcgcaat caaggtcatc tggatgttga ttgtcagcat
    18361 cataatcagg ataccaacga cgctgaccag agaggtaatc atttgggaga ggctttggtt
    18421 cagggtctga ctgatggtat ccacgtcgtt ggtcacacgc gagaggacct caccgtgggt
    18481 gatgtggtcg aagaaactca acggcaaacg gttaatcttt tcggctatct cgcggcggaa
    18541 gcggtaggtg atatctgcgg agatggaggt catcaggaag ccctggacaa ggtccaacat
    18601 cgcggagccg agataaagcg cgagcacgcg cagaaggaat tggctgattt ccgcgaagtc
    18661 tataccgcgc ggatcccccg cgatcatacg ggtgacgcct tcatacagcg cggttgttgc
    18721 ctgcccaaga atcttaggtc caaagatggt aaacaccact gaaacggacg tgatgaccat
    18781 caccagcagg atcgtccatt tgtacttgcc aagataactg agcagctgct tcatgctgcc
    18841 tttaaaattg cgtgcttttt caccgggcat gagcgcgcca ggtccactgc cgcggcggat
    18901 ggagggttgg ggagtacgcc ttatgggagc ttggttggtg gggctttccg acgttggctt
    18961 gttttcagat tgtggattgc tgttcatgcc aattcctcct tgctgagctg cgagctggca
    19021 atttcctgat aggtctggca ggtttttagc aattcctggt gcgtgccctg accaatcagc
    19081 ctgccgttat ccaaaaccag aatttggtcg gaatttttgg ctgttgcgac gcgttgcgtc
    19141 acaatcagta ctgtgctgtt gccaaccttc tttttcaggg cctggcgcag cgtggcgtcg
    19201 gtcttaaagt ccagggcgga aaaactgtcg tcaaaaatgt agattggcgg ctttttcacg
    19261 agcgcgcggg cgattgagag cctttgcttt tgaccacccg agaaattcgt gcctccctga
    19321 gcaacttcgg catctacgcc ttcgggcttg ccaaacacaa aatcagctgc ctgggcggtc
    19381 tcaatggctt cccgtaccgc ttccggatcc acgttgggct tgccgatgcg catattactg
    19441 tcgattgtcc cgctgaaaag cagccctttc tgcggcacaa aaccaatccg ctcgcgcaag
    19501 tcgtgctggt tcaggctgcg gatatccacg ccgtctatct ggatgctgcc tttgctgacc
    19561 tcaaagaagc gcgggatgag gttcacaacc gtggacttgc cactgccggt ggaaccaatc
    19621 agggatgtca cctgcccggg cagggcggta aagctgatgt cgtgcaaaac atccacctcc
    19681 gcgtcggggt agcggaaatc cacattcagg aattccacct tgccctgcac cggttccggg
    19741 agttttttag gttcagcggg gtcttggatg aaaatcggtg tctcaagcac atcagcaatg
    19801 cggtccccgg aaattgccgc gcgcggtatg aagatgaaca acatggaaag ggttaagaag
    19861 gagaacataa tctgggtggc gtattgcatg aaggcaatca ggttgccaac ctgcagggtg
    19921 gacgctgcca cttcgcgtgc gccgacccag atgattgcca cgctgagacc ggtcataatc
    19981 aggctgatga gcgggaacat agcggtcaac accctgccaa cgaagcgggt ggtgctggtt
    20041 aattcctggt tagctttatc aaaacgctgt tcttcaaaat cctgtttgtt gaaagcgcgc
    20101 accaccataa gaccggagag attctcgcgg ataaccaggt tcatgcggtc taccagcttc
    20161 tgcataatct tgaactttgg gagcgcgagc gtgaaagcaa gcatgatgaa catgatcaaa
    20221 atgcctaccg ccagtgcgat taaccaccac atattcggac ttttatcaat ggcgcggatc
    20281 accccgccga tacccattat cggagcgatg aaagccatgc ggatgaccat aaaaatcacc
    20341 tgttgcacct ggctgacgtc attggtcgtg cgtgtgatta gagttgaggc agaaaagttg
    20401 tcaaattcgg cgctggaaaa attggaaact ttttcaaaga ctgccgcgcg aatatctctg
    20461 gcaacgccgg ccgatgtgcg cgaggcaaga taactgacca tgatggaggt cacaaccgcc
    20521 agcaaggcca tcaaaatcat acggccgcca atgcgcagga tgtagttggt ttggttcttt
    20581 tgcaggttaa cgcccaacgt agagtattca ttaccgacgg cgcgcacagc catctgccgc
    20641 agaatagtgc cttccaggct gtccaggtgc tgcatgaggg ctgctttgat ctgctcgacc
    20701 tgtgcttccg gcagttgcgt aaataagctg agcgggtcgg tgcctgccgg caaggatttc
    20761 agtttttccg ccagctccgg tcccaccagg ctggaggctt gttccgggtt ctgttctatc
    20821 atctgaaagc cgtacaggat gaccagcggc ttggtcaacg ctttttcaag cgaagccgtc
    20881 gcagatgcat ccaggggctt gagcttgtag actggctgca gagaagcccc gggataatag
    20941 ggaaccaatg ccgcggattc cgacgtgccg ggctcaatcc gctcataagc agccaaagtc
    21001 tcagcgcgct cctgttcgga ttggaacagc gcgattttgt caagagaaga cgcgcggata
    21061 gtttccggga tagtcgtatc gacgccgttt tgctggatgc cgatgttgat aatctgagag
    21121 aggtaatccg gcagagccag gttggtgctt gccatcacat acagcaagcc caaagccaga
    21181 acaatgagca gccagtaagg tttgatataa ggtaagagtc gtttcataaa atggtccgct
    21241 tcgtgaaaat tatgagttgt tggtttgggt gaaaactttg ttcatacctt cgagcaagat
    21301 cttgaaagct ggctgcaggg cggcctgctg ttcaggggta aagccatcaa tcaggtcgtg
    21361 cagccaagcg cgcctggcct cgctggccgg cgccactttt tcctgtccta gattggtcag
    21421 gcaaaggcgt tttccgcgtc tgtccgcagg atccacctgg cggtttacca acccg
//
LOCUS       DAOQAD010000024        21218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_2549_length_21218_cov_6.317157, whole genome shotgun sequence.
ACCESSION   DAOQAD010000024 DAOQAD010000000
VERSION     DAOQAD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 21218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21218
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2549_length_21218_cov_6.317157"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            818..1375
                     /gene="frr"
                     /locus_tag="PKL60_03420"
     CDS             818..1375
                     /gene="frr"
                     /locus_tag="PKL60_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNW95218.1"
                     /translation="MIKELVKEAQQRMQAAVEVLEEDLTGIRTGRASPALIEKMPVEY
                     YGATLPLIQMATISVPESRQLLIRPFDASTLKNIERAILASELGLTPNNDGKSIRLNL
                     PPLTEERRRELVRVVHGRLEDCKVAVRNVRRDVIKDLKDFEKEKLISEDELNSAEEEL
                     KDLTAEMIDKIDEIGARKEKEILEV"
     gene            1380..2096
                     /gene="uppS"
                     /locus_tag="PKL60_03425"
     CDS             1380..2096
                     /gene="uppS"
                     /locus_tag="PKL60_03425"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNW95219.1"
                     /translation="MEAAEEPLKIPTHVGLIMDGNGRWANARGLPRLDGHRAGVENLR
                     GVITAGIKFGIKYLTFYAFSTENWNRPRDEVFGIMDLLAEFIDRETEPLHKEGVRILH
                     IGHLDRLRPSLVEKIKYAIHLTKDNRKITVLLAFNYGGRDEIVSAVRKIVASGVRPED
                     VNEALIGRNLFTAGIPDPDLIIRTSGEMRTSNFLMWQSPYAEWVFPATYWPDFNEDVF
                     RDMLREYSRRDRRFGGLSSK"
     gene            2096..2902
                     /locus_tag="PKL60_03430"
     CDS             2096..2902
                     /locus_tag="PKL60_03430"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNW95220.1"
                     /translation="MFWTRFKSALVFVPLILAVSYIGGAVYGLVLLAALGTGAWEYWR
                     LLKTMGYYVSLPLMLGGVLALLILRLFAGFAYMDLALVVILVAGAILSLKRFEMQDAQ
                     AALTFALHVSGIVYLGWLGGYFFSLRALPNGAWWTMLSVGLVMLVDLGAYAVGVKYGI
                     HKIFPRLSPRKSWEGYAGGILFGVGFGALLGWILTPVMPELTVWEGALLGLVIGTLAP
                     FGDLFISMLKRIAGVKDSGTLIPGHGGILDRTDTWIWAVMLGYYLARLFS"
     gene            complement(2954..4597)
                     /gene="pgm"
                     /locus_tag="PKL60_03435"
     CDS             complement(2954..4597)
                     /gene="pgm"
                     /locus_tag="PKL60_03435"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HNW95221.1"
                     /translation="MPIHPLAGKPAPLEALVNIPRLISAYYTLHPDPQNSAQAVVFGT
                     SGHRGSSLLGSFNEDHVLAISQAIYEYRKLAGISGPLFIGMDTHALSEPALYSAIEVF
                     AGNGIEIMIHADMRYTPTPVISHAILTYNAGRQSGLADGVVISPSHNPPDDGGFKYNP
                     PNGGPADTDTTSWIQNRANEIIRGGLKEVRRLGLAKALQADTTHPIDLISPYVNDLEN
                     VVNLKAVAASGIHIGVDPLGGSGLGYWEPIAERYALNIEVVNKRVDPTFSFMTLDKDG
                     KIRMDCSSPYAMANLVNMKDRFDIAFGNDVDFDRHGIVTPSVGLMNPNHYLAVAIWYI
                     FQHRPDWSRGAAIGKTLVSSSLIDKVALKIGRRLCEVPVGFKWFVPGLLDGSVGFGGE
                     ESAGASFLRKNGATWTTDKDGIILDLLAAEILAVTGKDPGWHYQALEAELGKSWYARM
                     DAPTGAREKAVLAALSPEMVKADMLAGDPITAKLTKAPCNNAPIGGLKVTTDFGWFAA
                     RPSGTEDIYKIYAESLKGEEHLARIQAEAREIVDAALAA"
     gene            complement(4608..5213)
                     /locus_tag="PKL60_03440"
     CDS             complement(4608..5213)
                     /locus_tag="PKL60_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNW95222.1"
                     /translation="MNVSEINVRDYRKNLVPVEDFIIKRWSARAMSAAALPQEELLRL
                     FEAARWAPSSGNFQEWYFLYAHRGTEQFDHFFSLLDIWNQEWCLRASVLLVVLSNRIS
                     PKGKEINTHAFDTGLAFENLALQGVAMGLIVHPMGGFDSERAVAELHIPDTYSVHCMA
                     AVGYPGEIGDLSEYNQNREYPNQRKDLKETYQEGGFPPVSE"
     gene            complement(5326..6066)
                     /locus_tag="PKL60_03445"
     CDS             complement(5326..6066)
                     /locus_tag="PKL60_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNW95223.1"
                     /translation="MKTKFSVLSIIVLLIGALTLAGCALFQAPQQTAAPTNVRTLSVN
                     GTGKVVLVPDIAYINIGVHTEAADVSSSLSNNNDLATAISTALQAEGVELKDIQTSNF
                     NVYPMQQYDNLGQATGTVYVVDNTVSITVRDLSKLGKLLDVAIRSGANNIYGITFDIA
                     DRESALASARDLAIKDAQSKAQAVAGVAGVQLGEVQAIVATDSSYVQPYSGYGMGGGG
                     ASALEPSVPVSAGQLIITYDVNLTYAIQ"
     gene            6222..7382
                     /locus_tag="PKL60_03450"
     CDS             6222..7382
                     /locus_tag="PKL60_03450"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HNW95224.1"
                     /translation="MPVNYPQIEQCLPAYARKEKGHQDALDAQHKQLWQLFSNPALEV
                     EAMREKITRAQALLRNLYCAKPVQEKLLTRKPLPALPSGYTLIAADGSQIVPNRHRPV
                     QFCVLNIGLIKIERGSGRAPEINVLSTLLEHESLFTPDGHLIGEEDVSLFRDLEERKA
                     LLEAANPQELPIITLTDGPLDVYENITTRRERKHIQEDVEKIHANLEARGVLSAGYID
                     KPGSELLSRMLSVLQVPDAELGAYDDTRREIRGISDAELLGNLLSAGERSAVFEAVTK
                     GEGKASLSVHFFYLNVSGGGEPNLARVEFPAWVSAHPRLVDTLHSVIYHEAMILDTHP
                     YPYALHRAHELAVISMPEHEQVEAMLVRELEQAGARVGKRSNKDWNKGLTGR"
     gene            7390..7998
                     /locus_tag="PKL60_03455"
     CDS             7390..7998
                     /locus_tag="PKL60_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95225.1"
                     /translation="MQEETKILIGKLLRADNRGFVAGCRTRDLVAPALGALCKVRLED
                     GLEIYGLIGNIVINDDSLVRQLVSGISIPTEYTEDNRHNRNLPVEINVHCVGFGLGGQ
                     IYHRLPPRPPLSLDALYLCDDEELVRFTSTGRYGYFRHILRLEDQPVEEILTAHLARA
                     RQAHAQKGRADWYTGACKELIVLLRDDYEGLMNVLNALAELE"
     gene            8006..9727
                     /locus_tag="PKL60_03460"
     CDS             8006..9727
                     /locus_tag="PKL60_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNW95226.1"
                     /translation="MDTMTGSQAHAIGYVVGGGLEANLQVRLTVPPQEVQEGSFVVID
                     SGGWRFYGLVTDLQLGSTNPRFADEQSEERLPRQMAELLHGQTLFTNLAVMPVLMMER
                     GPEPGTEEYRLWKDQHPEESKPIPVKTVPSHHSPVMLADAADIAQIFGEENNQEMFCI
                     GYTREQGHPVCLNLDKFVKRSSGIFGTTGSGKSFLARMILAGLIHADRASALIFDMHN
                     EYGPDSSSSDTGQAVPGLKGKFGAKIRLAGLGARTSFGKLNADLSLEIAQSDITSEDV
                     LLLSQALNLNETAATVLAALEREFGKEWFREFLGMETGTTEQDETGKSMPAPNSVEAW
                     AAGSNVHPKSALALHQKLGRLRVRPYLIADPATKGLDDIIENLENGRHVILSFGKYNT
                     ELDYLLVTNLITRKIRDHWEEKTEKFHSENSVEPRPLVVVVEEAHKLLNREMASQTIF
                     STIAREMRKYYVTLLIIDQRPSQIYDEVMSQLGTRISGSLGDEDDINAVLSGLPGRGA
                     LRGMLSKLQAKEEALLLGWGVPMPIPIRSRRYDNAFWQDLFHEERSPETFAGAYNALG
                     FGGDDPD"
     gene            9714..12284
                     /locus_tag="PKL60_03465"
     CDS             9714..12284
                     /locus_tag="PKL60_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNW95227.1"
                     /translation="MIPIKLSLSGFTSYREPVEIDFTGLDLVCISGQNGSGKSSLLDA
                     ITYALYGQARRRDEAIIHHASQKAEVSLEFAYEHQVYRVSRSITRGKGSQVEFYIRAE
                     NGPTPWKVLTERTMSETNARIQHTLRLDYETFINASFFLQGKADLFATQKPADRKRIL
                     SNILGLDSWEEYRKRAADLIQKRKGEVLLRDERLKEIQNELDEEPARHAKLQLLEEKL
                     KNARTETLLEQSALEQKRAVEQRLVSHRQMLALHEAQERKAAAARTETARRLNEKEAA
                     LAALQQKLASAESVEAAYKHLESLRKQLGELDALSERFHPLENRKRDLESRIALERQK
                     LLTEVKHLEEEEQQLEREEAANAARKAQQEQLHAQIAALEERASRKETLEQEAEMLQK
                     RLAELQAENAEIEKKGKELRDRLEKVQKVEGAECPLCGQPLALHDRERLEQELNEELL
                     TQREQYRLNQEEIKSAAQRQREITEERAQIQSFEKQIQSLTRQADQLGQQISLLAARA
                     EEFANTKALQLEHVRAALAEESYCAQERAERTEVLEKITALAYDPEAHARLREEARQA
                     EPARAAYNELQIARSTVGQQEQSLLELQETLEKQEEDLQRAAQERSKAAADLAEIESS
                     LPDIQVTEQSFQAKQDAEAGLQRQVGAARQELHILTELKARKISLREEKEQLNAEIAR
                     LKVLERAFSKDGVPAMLIEQALPELEEEANRVLGRLSNYSMSVRFNPQREFKDVRRAD
                     KMETLDITISDGSSTRDYETYSGGEAFRINFAIRLALARLLSRRSGAQLRTLVIDEGF
                     GNQDADGRQRLVEAIGRVADDFDMIMVITHIEELKEQFPNRIEVEKGPSGSRVSVIRG
                     "
     gene            complement(12265..13044)
                     /locus_tag="PKL60_03470"
     CDS             complement(12265..13044)
                     /locus_tag="PKL60_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95228.1"
                     /translation="MSAGAPIEAAWKELETFSSSADGLRLSEVRAIFAIGSLPGGYFR
                     PGQSDLDVLVLFQGAPGTEADFNKFQLAEKERLEALAAQAGPYEIELLFLHETRLQRD
                     PLTGRFPYADFVQRLLSQSKLLWGAFETGSLEAPGKEDFACAFRRYLEYLRKKHGGEL
                     QVFWSQGKLEELLNHTLALMRAYLQVQRGVSEYDKTRLAQRYRESQPAVPLPPALASA
                     LKARFAGAPISSEIAAAARAELPAFHAALAAQILRVNPESR"
     gene            complement(13044..14480)
                     /locus_tag="PKL60_03475"
     CDS             complement(13044..14480)
                     /locus_tag="PKL60_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW95229.1"
                     /translation="MQKPLRWYDSIFINIYYLGLTVRSQAFTPLILPLLVQQFVGDET
                     KGSSYGSIRLYSLMIALLVQAVMGMLSDRSTHRWGKRRPFLLASTVLETIAFIAIGVI
                     AAQMEGQAGYTALFAAVLLSMVFSNIGHGAAQGLIPDLVPENQRGRYSAIKTLFELPL
                     PLIFVSFFVAKMVKSGNYWAAIITVIATLIISTILAMFIRETPQEKAPAPMDWPAIGR
                     LVAMTALFTGVILLAGEAVKRLVPLAAGLPGLSRLLATGVIGAVGMLFAVAVGVILSM
                     RISLGSEAAQRPAFTWWVVNRLAFLVGANNLASFLLYFIQERFPELQGNAAAGPSAFL
                     VMIVGVAILAAALPGGWLTDKFGTKPCIAASGILATLGTAIVIASPAMSMMYVGGVLV
                     GLGVGIFYTANWALGTSLVPREQAGRYLGISNLAGAGAGAIGAYIGGPIGDGAGFVVL
                     MSIYGFLFLFSVLVLGRIKTGNARQEAA"
     gene            complement(14513..15028)
                     /locus_tag="PKL60_03480"
     CDS             complement(14513..15028)
                     /locus_tag="PKL60_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNW95230.1"
                     /translation="MNQRNEIITWDEVSRLIDHLLPQFNRVYTVMVMITRGGIIPGGM
                     LAEAMNIQNLFTASLDFPAEAHTPATPQSKFLTWPKFLQFPDASQLSGCDVLIVDDVW
                     GSGRTITAVKNRVQSSGGRASTCVLHFNPYRNLFGNMRPDYYAATTDAHIIYPWEMDR
                     GFQSKFFSGVL"
     gene            complement(15208..17745)
                     /gene="priA"
                     /locus_tag="PKL60_03485"
     CDS             complement(15208..17745)
                     /gene="priA"
                     /locus_tag="PKL60_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNW95231.1"
                     /translation="MAALDANPELKYAEVSINLAAVDRSYHYAIPPELQGKLQPGCLV
                     VVPFGRQIVQGVVLALLAETSVDKEIRPILSLNSEDTVLTPQQLELTHWLARETFAPL
                     GAAVNLMLPRGLSQRADFLAELKPDLTEIPTGLAPLQTRILKLLSERGSLRGAQLEHA
                     LPKLEWRGSLERLRKAGLVRTQSVFPPPRVSPKVVRTAQLTIPPEAVSSLDPKTLSSR
                     AETGARRLEILKLLASEAFPMDFSWIYAQTDSSYADLKTLADQDYLHFNETEVWRDPL
                     ENLQIENAHAPTLTPDQQRVWEQLQPRLGHPESQPVLLHGVTGSGKTEIYLRAVEQSL
                     AYGRQVIVLVPEIAMTPQAVRRYMARFPNRVGLYHSKLSEGELYDTWRRARKGELSII
                     VGARSALFVPLPDIGLIILDECDHESYDETGREPFYRAVETAEAYARICKANLILGSA
                     TPRVSQYYRAQKGDWQLLELPRRVLAHRDTLLKQAASLHTELLPAAGEEELLSLELPP
                     VDLVDMRAELKEGNTSVLSRPLQAGLEQVLARKQQAILFLNRKGSATYVFCRDCGQAL
                     LCPRDLSPLVYHQDRAGLLCHICGYTRGMPTKCPNCGSKQIRQMGIGTERLEALVQQA
                     FPQARLLRWDAETTTTRDAHELILSHFSAHRADILIGTQMLAKGLDLPLVTLVGIILA
                     ETGLNLPDYRAAERSFQVLTQVSGRAGRSPLGGRVVMQTYQPENYVIQAASRHDFAGF
                     YAQELAHRRELGYPPFTRLVRLEYRSPSEDKCRQEAELLAEILRTEIRALGMTRTDFI
                     GPVPCYTRKLFGRFRWQIILRGPDPTPMLHGLPLMGWTVEVDPPNLL"
     gene            complement(17745..18746)
                     /locus_tag="PKL60_03490"
     CDS             complement(17745..18746)
                     /locus_tag="PKL60_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95232.1"
                     /translation="MELRRSDHLPPRALLLSLLLLGVLFGSAACQPQYQGRPTAQPTR
                     VPSRTPTPTITSTLPPAPTATPSPSPTLTHTPTPLPSTTPTETPTPIPPRAAVLFPLK
                     AGGANTVNWSYFYLSRKELREDNSLLSLSATVAFQLLDRGIHSETVRVHDQDVTVYYL
                     RVSHVFNQVPIETKLVLTGYFGRAVPVSALPADGAAFVNLRTQNSDAVFEPWRIQSDW
                     LLPFEQRQALFQQMTLTDFEQLLADLPERVILLAHHPVIWPPDDYAQADLDMARVSAQ
                     AARLQAFFDHDAFDLLTGQSRMAEAWSAYLLHNQVIPTDVAQAGLSYSAEYLIIVIP"
     gene            complement(18750..19616)
                     /gene="amrB"
                     /locus_tag="PKL60_03495"
     CDS             complement(18750..19616)
                     /gene="amrB"
                     /locus_tag="PKL60_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNW95233.1"
                     /translation="MGSSEYIDVRPSPIAGSWYPGKAGELRAQVEAFLEKASPPALSG
                     EILGLVVPHAGLVYSGLTAAHAFKTLIGSSFSRVILLSPSHQYYREPLLTSGHEAYAT
                     PLGPVPVDREAVDALRTLLNGSRGPEITPIRRDQEHSLEIELPFLQVVLPQGFSLVPL
                     MLVNQSLDLVQALSDAINGYLSTRPREEKTLLVASSDLSHFYTEKQANQLDHRVLSAL
                     ETMDIFELFRLNTSGRGQACGMAPILTVLRACRNLGADKLVIADYRTSADVTHDSDSV
                     VGYGSAVITRTA"
     gene            complement(19705..20955)
                     /locus_tag="PKL60_03500"
     CDS             complement(19705..20955)
                     /locus_tag="PKL60_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNW95234.1"
                     /translation="MDNPIPRKPRFCQTHDIISLMESPNSPQSDEPIETEPGAPDRPL
                     QRLWARLIHLGIAERVLRVASALFTSLLLVGVLFVLSRFYASVSGSTPAEPLPAAEAL
                     TPTAPAAVPGISQKEALPAAEPQGIVPQVSLHTVLPSRGRTELLTYVIQAGDTLFSIA
                     EKFGLKPESILWGNRYTLGEDPHMIFPGQELLILPVDGTLHRWSAGEGLNGVAGYYGV
                     TADAIVNYPGNHLNPATLGDYALPNIAPGTLLIVPGGKGEFPDWRTPRITRENPATAV
                     NVGPGACSGSYGGVMGTLNFIWPVADHYLSGYDYNPEANHYGIDIHGEIGDPVSAADN
                     GVVVYAGWNDWGYGEMIVIDHGQGWQTLYAHLSTVDVSCGQEVYQGDTIGTVGTTGNS
                     SGPHLHFELRSDDYGRVNPWDFLR"
     gene            complement(20933..>21218)
                     /locus_tag="PKL60_03505"
     CDS             complement(20933..>21218)
                     /locus_tag="PKL60_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95235.1"
                     /translation="PDLRLRSVGLRWRSTRPTATFCRVALALHPTYGHVLSGCAAAPP
                     DLRLRSVGLRWRSTRPKIMLCRVALPLHPTCDSRIASGAYKRSDGQSNPA"
BASE COUNT         4436 a         6016 c         6738 g         4028 t
ORIGIN      
        1 cacatcagcg cgaatagtat gagcctggaa agggtgtttt tcatcgggtc ttcctcttgc
       61 ggtagtgcgt gccagcttgg gactgcaggc agcttgcgcc tgggcaaccc ttattgtact
      121 ccgaaaccct tggggcgggg cggtgcagag tggggttgtt ccgtcatcgg gagtcctgcg
      181 gtcaacccga caagctttgg ccagcgggtt tgtggagcca tcgttgggtt acgctcctgc
      241 gtcgctgcac ccaacctaca aacttaggtc gggtggagcg gcaggaaggt cgtaggtcgg
      301 gtggagcggc agcgcaaccc gacaagtcgt ggttgtaggt tgaggtggag cgagagcgca
      361 acccgacaaa ccgtgatcat aggtcgggtg gagcggcagc gcaacccgac aagctttggc
      421 cagcgggttt gtgcagcctt cgttgggttc cgcccccgcg cctctgcgcc cgccttaacc
      481 agcctgccag cctgagcgca tcttatcatc tgaatggagt gaaggatcct ggcgacatgc
      541 tgcaaaggct ttacgccggc ggctctgaac gtcaggaagt gtgtcaccct gcatgcagcg
      601 aaggaacttc ttctcttgct gacaagactc tgcgaccgcg gcttcgaatg acctgtccgt
      661 cgtggcgaga gccgccgcgg catggacgga tggcgatctg ccggttgact tcacccggat
      721 agggcgtctg ggatgggggc tgtaaagctc ctgcaggctg ttatttttcc cttttttaag
      781 gtatgcttaa ctttcgaaag cttatgagga ggcgaatatg atcaaagagc tcgttaaaga
      841 agcgcagcag cggatgcaag ccgccgtgga ggttttggaa gaagacctga ccggcatccg
      901 taccggccgg gcatcccctg ccctgatcga aaaaatgccg gtcgaatact acggcgcgac
      961 gcttccgttg attcagatgg caaccatcag cgtgccagaa tccaggcagc tgctcatccg
     1021 tcctttcgat gcgtccaccc tcaaaaacat tgagcgggcc atccttgcct ccgagctcgg
     1081 tctgacacct aacaacgacg gcaaatccat ccgcctgaac ctgccgcccc tcaccgaaga
     1141 acgccggcgg gaattggtgc gcgtggtgca cggccggctc gaagactgta aagttgctgt
     1201 ccgcaacgtc cgccgcgatg tgattaaaga cctgaaggat tttgagaaag aaaagctcat
     1261 atcagaggac gaactgaaca gcgctgaaga agaactgaag gacctaacgg ccgagatgat
     1321 tgacaaaatt gacgagatcg gcgcccgcaa agagaaagaa atccttgagg tctgaatcca
     1381 tggaagcagc ggaagaaccc ctgaagattc ccacccacgt gggcctgatt atggacggga
     1441 acggccgctg ggcaaacgcc cgggggctgc cgcgcctgga cgggcaccgc gctggcgtgg
     1501 aaaacctgcg cggcgtcatt acggccggca tcaaattcgg cattaaatac ctcacttttt
     1561 acgcgttctc cacggaaaac tggaatcgcc cgcgcgatga agttttcggg attatggacc
     1621 tgctggccga atttatcgac agggaaaccg agccgctgca caaagaaggc gtgcgcatcc
     1681 tccatatcgg gcatctggat cgcttgcgcc cttcgctggt ggaaaaaatc aagtacgcca
     1741 ttcacctcac caaagataac cggaagatta ctgtgctgct ggcttttaac tacggcggca
     1801 gggatgaaat cgtcagcgcc gtacgcaaaa tcgtcgccag cggcgtaagg cccgaagacg
     1861 tgaacgaagc cttaatcgga cgaaatctgt tcaccgccgg catccccgac ccggatttga
     1921 tcatccgcac cagcggcgag atgcgcacga gcaacttcct gatgtggcag agcccttatg
     1981 ccgagtgggt cttccccgcc acatattggc cggatttcaa cgaagacgtt ttccgggata
     2041 tgctgcgcga atacagccgc agagaccgcc gtttcggagg attaagctcc aagtaatgtt
     2101 ctggacccgt ttcaagtctg cgctggtgtt tgtgccgctc atcctcgccg tctcttatat
     2161 aggcggcgct gtttatgggc tggtcttgct ggctgccctg gggacaggcg cttgggaata
     2221 ctggcgctta ctcaagacca tggggtacta cgtatcgctt ccgctcatgc tggggggcgt
     2281 tttggcgctg ctgattttgc gactgttcgc cggctttgcc tacatggacc tcgcgctggt
     2341 ggtcattttg gtcgctggcg ccatcctcag cctgaagcgc tttgaaatgc aggacgcgca
     2401 agctgcgctg accttcgcgc tgcatgtatc cgggattgtg tatctcggtt ggctgggggg
     2461 ctactttttc tccttgagag cgctgccaaa tggagcctgg tggaccatgc tgagcgttgg
     2521 tctggtgatg ctggtggacc tgggcgcgta cgccgtcggg gtgaagtatg ggatacacaa
     2581 aatctttcca cgcctcagcc cgcgcaaaag ctgggaaggc tatgccggcg ggattctgtt
     2641 tggcgtgggt ttcggcgcct tgttgggctg gattctgacg cctgtcatgc cggagctgac
     2701 cgtctgggaa ggtgcgcttc taggtctggt aatcggaaca ctcgcaccct tcggggatct
     2761 cttcattagc atgctcaagc gcattgccgg cgtgaaggac agcggcacgc taatccccgg
     2821 gcacggcggt atcctggacc ggacagatac ctggatttgg gcggtaatgt tgggctatta
     2881 tctggcccgg ctcttttcct gagagatgaa aaaaccccgc gtttgaatgc ggggttgatg
     2941 tccatatgcg gtcctacgcc gcgagggcgg cgtccacaat ttcgcgcgct tccgcctgaa
     3001 tacgcgccag atgttcctcg cctttcaggc tttccgcgta aatcttataa atatcctccg
     3061 tgcccgaagg ccgcgcggca aaccagccga aatccgtggt caccttcagc ccgccaatcg
     3121 gggcattgtt gcagggcgct ttggtgagct tggcggtgat tgggtcaccc gccagcatat
     3181 ccgcttttac catctccggg gagagcgccg ccagaacggc tttttcgcgc gcgccggtag
     3241 gggcgtccat acgcgcgtac cagcttttgc ccagttcggc ttccagcgcc tggtagtgcc
     3301 agccggggtc cttgccggtt actgccagga tttccgcggc gagcaggtcc agaatgatac
     3361 cgtccttatc cgtagtccag gtggcgccgt ttttgcgcag gaacgaagcg cccgcgctct
     3421 cttcgccgcc aaagccaacg ctgccgtcca gcaaaccggg cacaaaccac ttgaagccga
     3481 ccggcacctc gcataagcgc cgtccgatct tgagggccac cttatcgatg agggagctgg
     3541 aaaccagcgt tttgccgatt gccgcgccgc gagaccaatc cgggcggtgc tggaagatat
     3601 accagatggc gaccgccaga taatgattgg ggttcatcaa tcccacgctg ggcgtgacga
     3661 tgccgtggcg gtcaaagtcc acatcattgc cgaaggcaat atcgaatctg tccttcatat
     3721 tgaccaggtt tgccatggcg tacgggctgg agcagtccat ccggattttg ccgtccttat
     3781 ccagggtcat aaaagagaag gtggggtcca cgcgtttgtt gacgacctcg atattcaggg
     3841 cgtagcgttc ggcgattggc tcccaatacc ccaggcctga accgcccagc gggtccacgc
     3901 cgatatggat accggaggcc gccacggctt tcaggttcac aacattttcc aggtcgttca
     3961 cgtaggggct gataaggtcg atgggatggg tggtatcggc ttggagggct ttcgcgaggc
     4021 cgagccgtcg gacctctttg aggccgccgc gaatgatttc gttggcgcgg ttctggatcc
     4081 agctggttgt atccgtatcc gcgggtccgc cgtttggggg gttgtacttg aagccgccgt
     4141 catcgggagg gttgtgagaa ggcgaaatga ccaccccatc cgccaggccg ctctgcctgc
     4201 cggcgttgta ggtgaggatg gcgtgggaga tgaccggcgt gggggtgtag cgcatgtccg
     4261 cgtgaatcat gatttctatg ccgttgccgg cgaacacctc aatcgcgctg taaagcgccg
     4321 gctcggagag cgcatgcgta tccatcccga taaagagcgg cccgctgatg ccggccagtt
     4381 tgcggtattc gtaaatcgcc tggctgatgg ccagcacatg gtcttcgtta aagctgccaa
     4441 gcagcgaaga gccgcggtga ccgctggtgc cgaataccac tgcctgggcg ctgttctgtg
     4501 ggtcggggtg cagcgtgtaa taggcggaaa tcaggcgggg gatattgacg agcgcttcga
     4561 gcggggcagg cttgcctgcc agagggtgga tgggcatggc ttctccttta ttctgaaaca
     4621 ggcggaaaac cgccttcctg ataagtttct ttcaagtcct tacgctggtt cgggtattcc
     4681 cggttctggt tatattcgga caaatcgccg atttccccgg ggtagcctac ggccgccatg
     4741 cagtgcacgc tgtaagtatc ggggatatgc aattcggcaa cggcgcgctc gctgtcaaat
     4801 ccgcccatcg ggtgaacaat caatcccatg gcaacgccct gcaaggccag gttttcgaag
     4861 gccaggccgg tatcaaacgc atgcgtgttg atttccttgc ctttgggtga gattcggttg
     4921 gagagcacga ccagcagcac gctggcgcgc aggcaccatt cctgattcca gatatccaac
     4981 agggaaaaga agtggtcaaa ctgttccgta ccgcggtggg cgtagaggaa ataccattcc
     5041 tggaaattgc ccgaagaagg agcccagcgc gcggcctcga acaggcgcaa aagctcttcc
     5101 tggggcagag ccgcagcgct catcgctctg gcagaccagc gcttgataat aaaatcctca
     5161 acaggaacca ggttcttgcg ataatcccgc acattgattt cagaaacatt catgagcttc
     5221 accttccggg gacgcacagg tcttgtggaa attttatcac agcgcgcacc tggtaaatgg
     5281 ccctgaaagc gctgctttca gggccttggg tgggacgcag atgggttact ggatggcgta
     5341 ggtcagattg acgtcgtagg tgatgatcag ctggcccgcg gaaaccggaa cggaaggttc
     5401 caaagcggag gctccgccgc cgcccatgcc gtacccgctg taaggctgca catagctgga
     5461 gtccgtggcg acgatggcct gcacttcgcc aagctgcacg ccggcgacgc cagccacagc
     5521 ctgggctttg ctctgggcat ccttgattgc caggtcgcgc gcgctggcca gagcgctttc
     5581 acggtcggca atgtcaaagg tgatgccgta gatgttgttg gcgcccgacc ggatggcgac
     5641 gtccaacagc ttgccgagct tgctcaggtc gcggacagtg atcgagacag tattgtccac
     5701 cacgtacacg gtgccggtgg cctgacccag gttatcgtac tgctgcatcg ggtaaacatt
     5761 gaaattcgac gtctggatat ccttcagctc cacaccttcc gcctggaggg cggtagagat
     5821 agcggtagcc aggtcgttgt tgttgctgag ggaagagctc acgtccgcgg cttcggtgtg
     5881 cacgccgata ttgatatagg caatatcagg caccagcacc accttgccgg tcccgttcac
     5941 gctgagcgtg cgtacgttgg taggagccgc ggtttgctgc ggagcctgga ataaggcaca
     6001 tccggctaac gtcagggctc cgatgaggag gacgataatt gaaagaactg aaaatttggt
     6061 tttcatggtt aactccttgg ttttcgctga aataaagacg cagcaggcgc ggggaatgtt
     6121 cccgaaaagc aaaacatctt ctcggaagaa gcgggaaaag cgccgggcat tggatataat
     6181 aaaaaccggg gttcttttca taacctctca gaaagagagc catgccagta aactatcctc
     6241 agattgagca atgcctgcct gcatatgccc gcaaggagaa aggccatcag gatgcgctgg
     6301 atgcgcagca caagcagctg tggcagttgt tttcgaaccc agccctggaa gtggaggcca
     6361 tgcgcgaaaa gattaccagg gctcaagctt tgctgcgcaa cctgtactgt gccaaaccgg
     6421 tgcaggaaaa gctgctgacc cgcaaacccc tgcccgcgct gccgtccggg tacacgctga
     6481 ttgccgcgga cggttcgcaa atcgtgccaa atcgccacag acctgtgcag ttttgcgtgc
     6541 tcaacatcgg cttgataaaa atcgagcggg gttcggggcg tgcgccggaa atcaatgtgc
     6601 tcagcacgct gttggaacat gaaagcctgt tcacccccga tgggcacttg attggcgaag
     6661 aggatgtgtc gcttttccgg gacctggagg aacgcaaagc cctgcttgaa gcagccaatc
     6721 cgcaggaatt gccgattatc accctcacgg acgggccctt ggacgtatac gagaacatca
     6781 ccacgcgcag ggaacgcaag catatccagg aggatgtgga aaaaatccac gccaacctgg
     6841 aagcccgcgg cgtgctcagc gcgggctata tcgacaagcc gggttcggag cttcttagcc
     6901 gcatgctcag cgttttgcag gttccggatg ccgaactggg ggcttatgac gataccaggc
     6961 gcgaaatccg cggaatcagc gatgccgaac tgctgggaaa cctccttagc gcgggtgaac
     7021 gctcggcggt gttcgaagcc gtcaccaaag gggagggcaa agccagcctg agcgtgcatt
     7081 tcttttacct gaatgtgagc ggcgggggtg agccaaacct ggcgcgggtc gaattcccag
     7141 cctgggtcag cgcgcatcca cgcctggtgg acacgctgca cagcgtgatc tatcacgaag
     7201 cgatgattct ggatacccac ccctatccgt acgcgctgca ccgcgcgcat gagctggcgg
     7261 tgattagcat gccggaacac gaacaagtag aagccatgtt ggtgcgtgag ctggagcagg
     7321 ccggcgcgcg ggtggggaaa cgctccaaca aagactggaa caaaggtctg acgggaagat
     7381 gagggagaga tgcaagaaga aacaaaaata ctaatcggga aactgctgcg cgcggataac
     7441 cgcggctttg tggcgggctg ccgcaccagg gatttggtcg ctccggcgct gggggcttta
     7501 tgcaaagtgc gtctggaaga cgggctggag atttacgggt tgataggaaa tattgtcatc
     7561 aacgacgaca gcctggtgcg ccagcttgtt tccggcatca gcattcccac ggaatatact
     7621 gaagataacc gccacaatcg caatttgccg gtcgaaatca atgtgcactg cgttggattc
     7681 gggctgggcg gccaaattta tcaccgcctg ccgccgcgtc cgcctctcag cctggacgcg
     7741 ctttacctgt gcgacgatga agagctggtg cgcttcacca gcaccggccg ctacggttac
     7801 ttccgccaca tcctgcgcct ggaagaccag ccggtggaag aaatcctgac agcgcacctg
     7861 gcgcgcgcca ggcaggcgca cgcgcaaaaa ggccgcgcgg actggtacac gggcgcctgc
     7921 aaggagctga ttgtgctgct gcgggatgac tacgaaggtc tgatgaacgt tttgaacgcg
     7981 ctggcagaat tggagtgagg tggatatgga cacaatgaca ggctcgcaag cgcacgcaat
     8041 cgggtacgtg gtcggcggtg gactggaagc caacctgcag gtgcgcctga ccgtccctcc
     8101 gcaggaggtc caggaaggct cctttgtggt catcgacagc ggcggatggc gtttttatgg
     8161 tctggtcacc gacctgcagt tgggctccac caacccgcgc tttgccgatg agcagagcga
     8221 ggaacgcctg ccgcgccaaa tggcggaact gctgcacggc cagacgctct tcaccaacct
     8281 ggcggtgatg ccggtgctga tgatggagcg cgggccggaa cccgggacgg aagaataccg
     8341 cctgtggaaa gaccagcacc cggaagaaag caaaccaatt cccgtcaaaa ccgtgcccag
     8401 ccaccactcg ccggtgatgt tggcggacgc cgctgatatc gcccagatat tcggggagga
     8461 aaacaaccag gagatgtttt gcatcggcta cacccgcgaa caggggcacc cggtctgcct
     8521 gaacctggat aagttcgtca agcgcagctc cggaattttt ggcaccaccg gctcgggcaa
     8581 gtctttcctg gcgcgcatga ttctggcggg gcttatccac gccgaccgcg cctccgcgct
     8641 gatttttgat atgcacaacg aatacggacc ggacagcagc tcctccgata ccggccaggc
     8701 agtgccgggg ctcaagggca agttcggcgc gaagattcgg ctggcgggtt tgggcgcgcg
     8761 gacttccttt ggaaagctga acgcggacct ctccctggaa atcgcgcagt cagatattac
     8821 cagcgaggat gtgctgctgc tcagccaggc gctgaacctg aacgagacgg ccgcgaccgt
     8881 gctggccgcc ttggaacggg aatttggcaa ggagtggttt cgcgaatttc tcggcatgga
     8941 aaccggcaca accgaacagg atgagaccgg taaaagcatg cccgcgccca acagcgtgga
     9001 agcctgggcg gctggaagca atgtgcaccc caaatccgcc ctggcgctgc accagaagct
     9061 gggacgcctg cgcgttcggc cctacctgat tgccgacccc gccacgaagg gcttggatga
     9121 tatcatcgaa aacctggaga acggcaggca cgtcatcctg tccttcggaa agtacaacac
     9181 cgagctggat tatttgctgg tcaccaacct gataacccgc aaaatccgcg accattggga
     9241 agaaaaaacc gaaaaattcc actccgagaa tagcgtggag ccgcgcccgc tggtagtggt
     9301 ggtggaagag gcgcacaagc tgctcaaccg cgagatggca tcgcagacca tcttcagcac
     9361 catcgcgcgc gaaatgcgca aatattacgt caccctgctt atcatcgacc agcgcccctc
     9421 ccagatttac gacgaggtca tgtctcagtt gggtacgcgc atttcgggct cgctggggga
     9481 tgaagacgat atcaacgcgg tgctctcggg tttgcccggc aggggcgcgc tgcgcggtat
     9541 gctctccaaa ctgcaggcta aggaggaagc gcttttgtta ggctggggag ttcccatgcc
     9601 gattcccatc cgctccagga ggtatgacaa tgcgttctgg caggacctct tccatgagga
     9661 gcgcagcccg gagactttcg ccggggctta taatgcctta ggatttggcg gcgatgatcc
     9721 cgattaaact ttccctctct ggttttacct cctaccgcga gccggtggag attgatttta
     9781 ccgggctgga cctggtgtgc atctccggtc agaacggctc gggcaagtct tccttgttgg
     9841 acgcgattac ctacgcgctg tacggtcagg cgcgccggcg cgacgaagct attatccacc
     9901 acgccagcca aaaagcggaa gtcagcctgg aattcgctta tgaacaccag gtctaccgcg
     9961 tatcgcgctc catcacgcgc ggcaaaggca gccaggttga attctacatc cgcgctgaaa
    10021 acggtccgac cccctggaag gtgctcaccg aacgcacgat gagcgaaacc aacgccagga
    10081 ttcagcacac cctgcgtctg gattatgaga ctttcatcaa tgcttcgttc ttcctgcagg
    10141 gcaaggcgga tttgttcgcc acacaaaagc cggcggaccg caagcgcatc ctgagcaata
    10201 tccttggtct ggacagttgg gaggaatacc gcaaacgagc tgcggacctt atccaaaaac
    10261 gcaagggcga ggtcctgctt agggatgagc gcctgaagga gattcagaac gaactggatg
    10321 aagagccagc ccggcatgcc aagctgcagc tgctggagga aaaactgaaa aatgcccgca
    10381 cagaaacgct tttggagcag tccgcgttgg aacagaaacg cgcagtggag cagcgcctgg
    10441 tcagccaccg tcagatgctg gccttgcatg aagcgcagga acgcaaagcc gccgccgcgc
    10501 gcacagagac tgcgcgccgc ttgaacgaaa aagaagcggc tctggctgct ctacagcaaa
    10561 aactggcatc ggcggaaagc gtggaagccg cctacaagca tttggagagc ctgcggaaac
    10621 aacttgggga gctggacgcg ctttccgagc gcttccaccc gcttgagaac cgcaaacgcg
    10681 acctggaaag ccgcatcgcg ctggaacgtc agaagttact gacggaagtg aagcacctgg
    10741 aagaggagga gcagcagctg gaacgcgagg aggcggccaa tgccgcccgt aaagcccagc
    10801 aggagcagct tcacgcgcaa atcgccgcgc tggaagagcg cgcgagccgc aaagaaacgc
    10861 tggaacagga agccgaaatg ctgcaaaagc gccttgctga gctgcaggca gaaaacgcgg
    10921 agattgaaaa gaaaggcaag gagctgcgcg accgcctgga gaaggtccag aaagtagagg
    10981 gcgcggagtg ccccttgtgc ggccagccgt tggccctgca cgaccgcgag cgtctggagc
    11041 aggagctgaa cgaagagctg cttacccagc gggagcagta caggctaaac caggaagaaa
    11101 taaagagcgc cgcccagcgc cagcgtgaaa tcacggaaga acgcgcgcaa attcaaagct
    11161 tcgagaagca gattcaatcg ctcacccgcc aggcggacca actgggtcag cagattagtt
    11221 tgctcgctgc ccgcgcggag gagttcgcca ataccaaggc acttcagttg gagcacgtgc
    11281 gcgcggccct ggcagaagag agctactgcg cgcaggaacg ggcggaacgg acggaagtgc
    11341 tggaaaaaat taccgcgctg gcttatgacc cggaagcgca cgcccgcttg cgggaagagg
    11401 cgcgccaggc agagcctgcc cgagcggcgt ataacgagct gcagattgcc cgcagcacgg
    11461 tggggcagca agagcaaagc cttttggaac tgcaagaaac cctcgaaaag caggaggaag
    11521 acctgcagcg cgccgcgcag gaacgctcca aagccgccgc ggatctggcg gaaattgaaa
    11581 gcagtttgcc ggatattcaa gtgaccgagc agtcattcca ggcgaagcaa gacgccgagg
    11641 cggggctgca gcgccaggtg ggggcagcgc ggcaggaact gcacattctg accgagctga
    11701 aggcgcgcaa aatcagcctg cgggaagaga aagagcagct gaacgcggag atagcgcggc
    11761 tgaaggtgtt ggagagggca ttttccaagg acggcgtgcc ggcgatgttg attgaacagg
    11821 ctctgccgga gttggaggaa gaagccaacc gcgttctcgg ccggctttcc aattacagca
    11881 tgagcgtaag gtttaatccc cagcgggaat tcaaggatgt caggcgcgcc gacaagatgg
    11941 aaacgctgga tatcaccatc agcgacggct cctccacgcg ggattatgag acttactccg
    12001 gcggggaagc cttccgtatc aattttgcca tccggctcgc gctggcgcgc ctgctctcaa
    12061 ggcgcagcgg cgcgcaactg cgcacgctgg tcattgatga gggctttggg aatcaggacg
    12121 ctgacggcag acagcgcctg gtggaagcaa tcggccgggt agcggatgac tttgacatga
    12181 ttatggtcat tacgcacatc gaggaattga aagagcagtt cccgaaccgg attgaggtgg
    12241 aaaaaggccc gtccggttcg cgggtcagcg tgattcgggg ttgacccgca ggatttgcgc
    12301 cgccagcgcg gcgtgaaaag caggcagctc cgcgcgcgcg gcggctgcga tttcgcttga
    12361 aattggcgcg cccgcgaacc gggctttcag agcgctggcc aaagcggggg gcagcggcac
    12421 agccggctgg ctctcgcggt agcgctgggc gaggcgggtt ttgtcgtatt ccgacacccc
    12481 gcgctgcacc tgcaggtacg cgcgcatcag cgccagcgtg tggttcagca gttcctccag
    12541 cttgccctgg ctccaaaaaa cctgaagttc gccgccgtgc tttttccgca gatattccaa
    12601 atagcgccgg aacgcgcagg caaagtcctc tttccccggc gcttccaggg aaccggtctc
    12661 gaacgcgccc cacagcagct tactttgtga gagcaaacgc tgcacaaaat cggcgtaggg
    12721 gaagcggccg gtcagcgggt cgcgctgcag gcgggtttcg tgtaaaaaga gcagttcgat
    12781 ctcgtaaggc ccggcctggg ctgccagcgc ttccaggcgt tctttttcag ccagttggaa
    12841 tttgttgaaa tcagcttccg tgccaggcgc gccctggaag aggaccagca cgtccaggtc
    12901 gctttgcccg ggccggaagt agcccccggg cagagagcca atggcgaaaa tcgcgcgcac
    12961 ctcgctcagg cgcagcccgt cagcagagct ggaaaatgtc tcgagctctt tccaggccgc
    13021 ttcaatcgga gcgcccgcgc tcatcaggct gcctcctggc gggcatttcc ggtctttatc
    13081 cgccccaaca ccagcactga aaacaggaac aggaagccgt agatactcat caacaccaca
    13141 aaaccagcgc catcgccaat cgggccgccg atatatgccc cgatggcgcc agcgccagcc
    13201 ccagccaggt tggaaatccc caggtagcgc ccggcttgtt cacgcgggac caggctggta
    13261 cccagcgccc agttggcggt gtagaaaatg ccaactccca aacccaccag aaccccgccg
    13321 acgtacatca tggacatagc cggcgaggca atcacaatgg ctgtgccgag cgtggcaagg
    13381 atgccgctgg cagcgatgca cggcttggtg ccgaatttat ccgtcagcca gccgcccggc
    13441 agcgcggccg ccagaattgc cacgcccaca atcatcacca ggaaggctga agggcccgcc
    13501 gcggcattcc cctgcagttc ggggaagcgt tcctgaataa agtacagcaa aaagctggcc
    13561 aggttgttcg ccccgaccag gaaagccaga cggttgacta cccaccaggt gaaggccggc
    13621 ctttgggcgg cttcggagcc cagactgatg cgcatggaga gtatcacccc gaccgcgacg
    13681 gcgaagagca tgccgaccgc gccaatcaca ccggttgcga gcaagcggga aagcccgggc
    13741 agccccgccg ccagcggcac caggcgtttg accgcctcac ccgccagcag gatgacgccg
    13801 gtaaacaggg cagtcatagc taccaggcgt ccgattgccg gccagtccat cggcgcgggg
    13861 gccttctcct gcggggtttc gcggataaac atcgccagga tggtgctgat aatcagcgtg
    13921 gctatgacgg tgataattgc cgcccaatag ttgccggatt tcaccatttt ggcaacgaaa
    13981 aaggagacaa agataagcgg aagcggcagt tcaaagagcg tcttgatagc ggaatatcgc
    14041 ccgcgctgat tctccggcac caggtccggg atgagacctt gggcggcgcc gtgcccgatg
    14101 ttggagaaga ccatggagag cagaaccgcg gcaaagagcg ccgtgtaacc cgcctggcct
    14161 tccatctggg cggcaatcac gccgatggcg atgaaagcaa tcgtctccag cacggtgctg
    14221 gccagcaaaa acggccggcg cttgccccag cggtgggtgc tgcggtcgga aagcatgccc
    14281 atcacagcct ggacgagcag ggcaatcatc agggaataca ggcggatgct gccgtagctg
    14341 ctgcccttgg tctcatcgcc gacgaattgc tgaacaagca gcggcaggat aagcggggtg
    14401 aaggcttggc tgcgaacggt cagcccgagg tagtagatat taatgaagat gctgtcgtac
    14461 cagcgcaggg gcttttgcat gaaacctccg gacgggcgcg agggatattc cctcagagca
    14521 cgccgctgaa aaatttggat tgaaaaccgc ggtccatttc ccatgggtag atgatatgcg
    14581 cgtcggtggt agccgcgtaa taatcagggc gcatgtttcc aaacaagttg cggtaggggt
    14641 tgaagtgcag cacgcaggtg gaggcccgtc cgccggagga ctggacgcgg tttttgacag
    14701 cggtaatggt gcgcccggaa ccccaaacgt cgtccacgat gagcacatcg cagccgctca
    14761 gttggctggc atccgggaat tgcaggaact tgggccaggt caggaatttg gattgcgggg
    14821 tggcgggggt gtgcgcttcg gcggggaagt ccaaggaagc ggtaaagagg ttctggatgt
    14881 tcatggcttc ggcgagcatg ccgccgggaa tgatgccgcc gcgcgtgatc atcaccatga
    14941 cggtgtagac ccgattgaac tgcggcagca ggtggtcaat cagtcgggag acttcatccc
    15001 aggtaattat ttcattgcgt tgattcattt ccgcaccaat cttgacgggg attgttcatc
    15061 ttcccgtcgt cagggcctgc atcccgtcgt tgcgaagcag cgccagcagc cgaagcaacc
    15121 tcaccgtttg ccaggcttaa caggaagatt gcttcgcaag ccctgcataa caaagctctc
    15181 gcgatgacga acacaccctt cagaatatta tagcaggttc ggcgggtcca cttccaccgt
    15241 ccagcccatc agcggcagcc catgcagcat cggggtcggg tccggcccgc gcaggatgat
    15301 ttgccagcgg aagcggccga acagcttgcg ggtatagcat ggcaccggtc cgatgaagtc
    15361 cgtccgcgtc atccccagcg cgcgaatctc cgtgcgcaaa atttctgcca gcagttccgc
    15421 ttcctgccgg cacttatcct cagaagggct gcggtactcc agacgcacca ggcgcgtgaa
    15481 gggcgggtaa cccagctcgc ggcggtgcgc cagttcctgc gcgtaaaaac cggcaaagtc
    15541 gtgccgggaa gccgcctgga tgacataatt ttcgggctgg taggtttgca taactacgcg
    15601 cccgcccagg gggctgcgcc ccgctctgcc gctgacctgg gtgagcacct gaaagctgcg
    15661 ctccgccgcg cggtaatccg gcaggttcag tccggtttcc gccaggataa ttccgaccag
    15721 cgtcaccagc ggcaggtcca gacccttggc aagcatctgc gtgccaatca ggatatccgc
    15781 gcggtgagcg ctgaagtgcg agaggatgag ctcatgcgcg tcgcgcgtgg tggtggtttc
    15841 cgcgtcccag cgcagcaagc gggcctgggg gaaagcctgc tgaacgagcg cttccaggcg
    15901 ttccgtaccg atgcccatct ggcggatttg tttgctgccg caattcgggc acttggtcgg
    15961 catgccgcgc gtgtaaccgc agatgtggca gagcagaccg gcgcggtcct ggtggtagac
    16021 cagtgggctc aggtcgcgcg ggcagagcag cgcctggccg caatcccggc aaaacacata
    16081 ggtagcgctg cccttgcggt tgaggaagag gatagcctgc tgcttgcggg cgagcacttg
    16141 ctccaacccg gcctgcagcg ggcgggaaag cacgctggtg ttgccttcct tcagttccgc
    16201 gcgcatatcc accaggtcca ccggcggcag ctccaggctc aacagctctt cctcaccagc
    16261 cgccgggagg agctcggtgt gcaggctggc agcctgtttc agcaaggtgt cgcggtgcgc
    16321 caggacgcgc ctgggtaatt ccagaagctg ccagtcgcct ttctgcgcgc ggtaatactg
    16381 gctgacgcgc ggggtggcgg agcccaatat caggttggct ttgcagatgc gcgcgtaggc
    16441 ttccgcggtt tcgacggcgc ggtagaacgg ctcgcgcccg gtttcgtcgt aggattcgtg
    16501 gtcgcactca tccaggatga tgagaccgat atccggtagt gggacgaaca gcgcgctgcg
    16561 cgccccgacg atgatcgaaa gttcgccttt gcgcgcgcgc cgccaggtat cgtacagttc
    16621 gccttcagac agcttggagt ggtacagccc gacgcggttg gggaagcgcg ccatgtagcg
    16681 gcgcacagcc tggggggtca tggcaatctc aggcaccaaa acgatcactt gccgcccgta
    16741 cgccaggctt tgctccaccg cacgcaggta aatctcggtc ttgcccgagc cggtgacgcc
    16801 gtgcagcagc actggctggc tttcgggatg cccgagccgc ggctgcagct gctcccacac
    16861 gcgttgctgg tccggcgtga gcgtgggggc atgcgcgttt tcgatttgca ggttttccag
    16921 cgggtcacgc cagacctcgg tttcattgaa gtgcaggtag tcctggtctg cgagtgtttt
    16981 caggtccgcg taagagctgt cggtttgggc ataaatccag gagaagtcca tcgggaaggc
    17041 ttcggaagcc aacagcttca ggatctccag gcggcgcgcg cctgtttccg cgcgcgagct
    17101 gagcgttttg gggtccaggc tgctcaccgc ttcaggcggg attgtcagct gggcagtgcg
    17161 cacgaccttc gggctcacgc gcggcggggg aaagacgctt tgggtgcgca ccagtccggc
    17221 tttgcgcagg cgctccaggc tgccgcgcca ttccagcttg ggcaaggcat gttccagctg
    17281 ggcgccgcgc aaagacccgc gctcggaaag caatttcagg atgcgggttt gcaggggcgc
    17341 cagaccggtt gggatttctg tcaggtcagg cttcaattcc gccaggaaat ccgcgcgttg
    17401 ggagagcccg cgcggcagca tgaggttcac ggcggcgccc aacggggcga aagtctcgcg
    17461 cgccaaccaa tgcgtcagct ccagctgctg cggggtgagc acggtatctt ctgaattcag
    17521 tgagaggatc gggcggattt ctttgtccac gctggtttct gccagcagcg ccagcaccac
    17581 gccctgaacg atttgcctgc cgaaaggcac caccaccagg cagcccggct ggagtttgcc
    17641 ttgcagttcc ggggggatgg catagtgata gctgcggtct accgccgcga gattaatgct
    17701 gacttccgcg tactttagtt ccgggttggc gtccagggca gccatcaggg tatcacgatg
    17761 atcaggtatt cagccgagta ggacagcccc gcctgggcaa cgtcggtcgg gataacctgg
    17821 ttgtgcagca ggtaggcgct ccaggcttcc gccatccggc tttgcccggt cagcaggtcg
    17881 aacgcgtcgt ggtcaaaaaa ggcttgcaac cgcgcagctt gcgcgctgac acgcgccata
    17941 tccaaatctg cctgggcgta atcgtccggg ggccagataa cggggtgatg cgccagcaga
    18001 atcacgcgtt cgggcaggtc tgccagcagc tgttcaaaat cggtgagagt catctgctga
    18061 aagagagcct ggcgctgttc gaagggcagc agccagtcgg attgaatgcg ccagggctcg
    18121 aacacagcgt cggagttctg agtgcgcaga ttcacaaacg cggcgccgtc cgcgggtaag
    18181 gcgctcacgg gaacagcgcg cccgaagtaa ccggtgagca ccagtttggt ctcaatcggc
    18241 acctggttga agacatggct gacgcgcagg taatacacgg tcacatcctg gtcgtgcacg
    18301 cgcaccgtct cggaatggat gccgcggtcc aacagttgaa acgcgaccgt agcggagagg
    18361 ctgagcaggc tgttatcttc gcgcagctct ttgcggctga ggtagaaata gctccagttg
    18421 acggtattcg caccgccggc tttgagcgga aagagcacag ccgcgcgcgg agggatgggc
    18481 gtgggggttt cggttggggt ggtgcttggc agaggagtgg gcgtgtgggt cagtgtcggg
    18541 ctgggcgagg gggtggcggt tggcgccggg ggaagcgtgg atgtgatggt gggcgtaggc
    18601 gtccgggaag ggacgcgcgt gggttgggcg gtggggcggc cttggtattg cggctggcaa
    18661 gcggcgctcc cgaacagcac tccgagcagc agcaggctta acagcaaagc acgcggtgga
    18721 aggtggtctg aacgccgcaa ctccaccgcc tacgctgtcc tggtgataac ggccgagccg
    18781 taacccacca cagaatccga gtcgtgcgtg acgtcagcgg aggtgcggta atccgcaatg
    18841 acaagcttat ccgcgcccag gttgcggcag gcgcgcagca ccgtcaggat gggtgccatc
    18901 ccgcaagctt gtcctcgacc gctggtattc agcctaaaaa gttcaaaaat gtccatcgtt
    18961 tccaacgcgc tgagcacccg atgatccagt tggttggctt gtttttcggt gtagaagtgc
    19021 gaaaggtcgc tggatgccac cagcagggtc ttttcttcac gcgggcgcgt cgacagatat
    19081 ccgttaattg catcggagag cgcctgaacc aaatccaggg attggttaac cagcatcagc
    19141 ggcaccaggc tgaagccctg cggcagtacc acctgtaaga aagggagctc aatttcgagc
    19201 gagtgttcct ggtcgcgccg tataggcgtg atttccggtc ccctgctccc attcaggagc
    19261 gtgcgcaggg cgtctactgc ctcacggtcc accggcaccg gaccaagcgg ggtggcatag
    19321 gcttcatgcc cgctggtgag taaaggttcg cggtaatact ggtgcgaggg ggagaggagg
    19381 attacgcggg aaaagctgct tccgatgagc gttttaaagg catgcgcggc tgtgaggccg
    19441 gaatagacca ggccggcatg cggaacaacc aaacccagga tttctccact tagcgctggc
    19501 ggggaagctt tttccagaaa agcttcgacc tgggcgcgca gttccccggc tttgcctggg
    19561 taccagctgc ccgcgattgg agatgggcgt acgtcgatat actcagagct tcccatatca
    19621 gtattatagc gcaagcgaat gcccgtgcag cagttcccgg gcagggatta tgcttgcctg
    19681 gagtgattca gccggaaaag cgggttaacg caagaaatcc cagggattta cgcgcccgta
    19741 atcgtcgctg cgcagttcaa aatgcaggtg cggaccgctg gagttgccgg tggtgcccac
    19801 ggtgccgata gtatcccctt gataaacttc ctgcccgcag gaaacgtcca ctgtgctgag
    19861 gtgcgcgtag agcgtctgcc agccctgacc gtggtcgatg acgatcatct cgccgtagcc
    19921 ccagtcattc cagccggcgt ataccacaac gccgttatcc gcggcagaga cggggtcgcc
    19981 aatctcgccg tggatatcga tgccgtagtg gttggcttcc gggttgtaat cgtagccgga
    20041 aaggtagtgg tccgctaccg gccagataaa gttgagcgtg cccattacgc cgccgtacga
    20101 gccactgcaa gcgccggggc cgacgttcac tgcggtagcc gggttctcgc gcgtgatgcg
    20161 cggcgtgcgc cagtccggga attcgccttt gccgcccgga acgatgagca gtgtacccgg
    20221 ggcgatgttc ggcagggcgt agtcgcccag ggtcgcgggg ttcagatggt tgcccgggta
    20281 gttcacgatt gcgtcagccg tcacgccgta atatcctgcc acgccgttca ggccctcgcc
    20341 ggcgctccag cggtgcagcg tgccgtccac gggaagaata agcaattcct gcccggggaa
    20401 aatcatatgc gggtcttccc ccagagtgta gcggttgccc cacagaatgg actcgggctt
    20461 gagaccaaat ttttccgcaa tggaaaaaag cgtatctccg gcctggatga cgtaagtgag
    20521 cagttcggtg cgtccgcggg agggcaggac ggtgtgcaag gagacttgcg gcacgatgcc
    20581 ctgcggttca gcggcgggca gcgcttcttt ttgggaaatg ccaggaactg ctgccggcgc
    20641 ggtgggggtg agcgcttctg cagccgggag cggctcggcg ggagtgctgc cgcttacgct
    20701 ggcatagaat cggctgagca caaagaggac cccgaccagc agcaggctgg taaatagagc
    20761 cgatgccacc cgcaggacgc gttcggcaat gccgagatgt atcagcctgg cccacagacg
    20821 ctgaagcggg cgatcgggag cgcctggttc tgtttcaatt ggttcgtcgc tttgcggaga
    20881 gttaggactt tccatcaggc tgataatatc atgagtctga caaaagcgtg gtttacgcgg
    20941 gattggattg tccatctgag cgcttgtatg ccccgctcgc aatcctggag tcgcaggtcg
    21001 ggtggagcgg cagcgcaacc cgacagagca tgatcttggg tcgggtggag cgccagcgca
    21061 acccgactga gcgtagccgt aggtcgggtg gagcggcagc gcaacccgac agaacgtggc
    21121 cgtaggtcgg gtggagcgcc agcgcaaccc gacaaaacgt ggccgtaggt cgggtggagc
    21181 gccagcgcaa tccgacagaa cgtagccgta ggtcgggt
//
LOCUS       DAOQAD010000025        20332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_2725_length_20332_cov_5.050747, whole genome shotgun sequence.
ACCESSION   DAOQAD010000025 DAOQAD010000000
VERSION     DAOQAD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 20332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20332
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2725_length_20332_cov_5.050747"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PKL60_03510"
     CDS             <1..277
                     /locus_tag="PKL60_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNW95236.1"
                     /translation="EIVEAMQAPLEEIAATAKRVLEKTPPELISDIIDRGVALCGGTA
                     LMKGLDKYLTVALGIPAYIVDNPVTCTVEGTAKAFGVLDLILRSQQN"
     gene            285..1049
                     /locus_tag="PKL60_03515"
     CDS             285..1049
                     /locus_tag="PKL60_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95237.1"
                     /translation="MEASQAPELQPDAVFTDKKKRRIRAWRFVFSLLLLAGLCASGYH
                     AWQWFAPRAARYGLSILWINGKLILILLPVVLVLLFWLLFANLPRKKTIISLSEDGIR
                     KKNGRRDLKLRWDSLETLRFKFSRAFFLGIPGSRRERVELTDAVGNKLCFDARMDRFE
                     ELVNRIRERSFPALYQLSQERLSRAGKLAFGKQVSLSPDSLQIRKTSLPLTQIKSAAI
                     EKGCLRIKTINNRKKRIRVDKLDNPDVLLHLLGKST"
     gene            complement(1046..1741)
                     /locus_tag="PKL60_03520"
     CDS             complement(1046..1741)
                     /locus_tag="PKL60_03520"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNW95238.1"
                     /translation="MCDDNIFFLTCRLDERKQRSGRMEKAKIPDIVVERLPVYLQALN
                     HLLREGRGVVSSAELGRMVDVSAAQIRKDLSYFGGFGKQGSGYHVISLIENLRAILHL
                     NQIWPVVIVGVGNLGQALLRYQGFARRGFEIVLACDNNPDLIGSTQSGMQINDVRNLA
                     DDLKRLNIQVAVLTVPAGCAQQVTDQLVSGGIRAILNYAPLVLKVPESVHVINIDPVV
                     KLQTLTFYLEAED"
     gene            1841..2209
                     /locus_tag="PKL60_03525"
     CDS             1841..2209
                     /locus_tag="PKL60_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95239.1"
                     /translation="MKAARTILYIVAGILIVLSFLFILGAFSPSGSTSWLTVGLIGLA
                     IAFVFIIIGARIGAKAAGTTQNISVNVDLPGNVKMDAIKCQSCGSPLAADDIKMIQGA
                     PVVTCHSCGTTYQLTEEPKW"
     gene            2215..3972
                     /locus_tag="PKL60_03530"
     CDS             2215..3972
                     /locus_tag="PKL60_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95240.1"
                     /translation="MKKLNTVFLLLVLLAAFMFPAAVSAQTYRFTVSEYEVEAYLEQD
                     GSLTLRYLMVFENDPSADPIDFIDLGLPLAQYDLHNITGTINGETIPRIGDSAYVNGA
                     ELALQDLAIQPGETGTVIATVTGITGVLFPYDQGDRENYINFQFSPNTFDAEFDRSTA
                     TKYRMTIVLPPGVGIDAGVYYRPSGWPGSDTPEASTTQDGRVYYSWYTENANLHSNYY
                     FGTAFPASAVPTGAVSSKEEYTQQGGETSSGSGGFSIDPGTIFCCGGSLIFGAFVVLG
                     IIKGSKAESKRKMAYMSPKISIEGQGVKRGLTAVEAGILMEEPLDKILTMIMFGLLKK
                     EAITITQKDPLTIKPSEPLPDGLYDYEKGFIQAFLKEKPSERKQALQTMIIDLVNSVT
                     AKMKGFSKAETVAYYKDIIARAWKAVQDANTPEIKSAQYDHTLEWTMLDKDFNTKTEE
                     TFSGGPVIVPMWWPRYNPGWQPSAQTSGGGGLNLPASAGTGAPSGGGSKPSLSLPNIP
                     GSDFAASMINGASSLAAGAIGDLTGFTGAITNKTNPIPTTSYKSGSGGFRGGGGGGHS
                     CACACACAGCACACAGGGR"
     gene            3987..5339
                     /locus_tag="PKL60_03535"
     CDS             3987..5339
                     /locus_tag="PKL60_03535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNW95241.1"
                     /translation="MAHTLLPKKPLPPAAPGLYQFDIADDDSKSRVHIRLDEDGSGLL
                     VVNASRVVHLNPTAVQMAYYHLSKAGEKDAVRALSKLYKAPKNRISEDYARISNQVDA
                     LLDEQSGLCPVCDLGLEKHMPFSVALSAPYRMDLALTYRCNNSCAHCYNARPRSYPEL
                     DTRTWKEILDRVWELRIPHVVFTGGEPTLREDLVELVAYAQELGLVTGLNTNARRLAD
                     EQLMQDLVAAGLDHVQITVESHNPAVHNAMVRAAGAWDETVAGLRNAVHSGIYLMTNT
                     TLLRNNTSDLEEILNFLADEKVPTVGLNALIYSGHGATVNTGLAEVELPPLLELATRL
                     TRQHDQRLIWYTPTQYCHFNPILLSLGIKGCTAALYNMCVEPDGQVIPCQSYYEALGH
                     ILDDDWDRIWNHPLSRELRERKNIPEGCQQCDFLVECGGGCPLARAHQSVQAITADIF
                     "
     gene            5350..6486
                     /locus_tag="PKL60_03540"
     CDS             5350..6486
                     /locus_tag="PKL60_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HNW95242.1"
                     /translation="MIDFIKRLFSKEPAHLPLEPGFHAYHSPKDDPQPYRLHLRIDPD
                     GEGGLIVNASSVLHLNQTAAEFAYYLIKGKQDAEITRLVSERFSAPIETIAADVVSFH
                     QQLLSFIRKTDQEPITSFGFEPHTQLKDISAPYRLDCCLTKSCETPSEPALLSDELDS
                     SAWKKMIRKAFEAGIPHLIFWGGEPTLRADLPELLAYCEELGLVTGLVSRGSKLNDAA
                     YVQSLIDSGLDHLFVPFQPGDDSLLSALRQILPLDLYTCVGLLLHADTESRQTIEELR
                     VLGVNAFALIPLSTDAAPAIDRAEEAVLESGLPMINDLPLPLDLRAALPEQNFTAAPD
                     PEAEFVTLELAPNGDLRTLPMYHKYLGNLLTDSWRDLWQQRYQA"
     gene            6488..7228
                     /locus_tag="PKL60_03545"
     CDS             6488..7228
                     /locus_tag="PKL60_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNW95243.1"
                     /translation="MNIHTWHSRYVLQALWTAKLRQFLLNKVNARPGEQILEVGSGTG
                     ALFPDFENRGLRVVGLDYKPKRCQYSLAYNSQRTVICGNAYAIPAADQSFDLCVCHYL
                     LLWLKEPERALAEMARVTRRGRHVIAFAEPDYQSRIDYPEVFQKIGEVQNRSLEFQGV
                     DLSIGRRMGAMFRGLGLQNVNVGLLAGEWKEPDPQVFEAEWDIIAYDLGGLAPVDEIL
                     AFKAKARETWLSGQATVFIPTFYAYGMV"
     gene            7270..8724
                     /locus_tag="PKL60_03550"
     CDS             7270..8724
                     /locus_tag="PKL60_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HNW95244.1"
                     /translation="MKKTKLGLLLVSLILLFSAPLSSAAGQTQTCPTNSSVAEILNQV
                     DSAYITKWIRDFSGEDFVAIGGAQRRILTRQSLQLFNFNANALAYPYLGELLRSFGYE
                     DGTSLADHLYTPTLKLTSEGIPSQIFDERGRPIVLIPELMDEQAQAASWKNKIVILPG
                     HGAHADELVLMTAHMDSTSGSASTNAPGAEDNASGIAALMEAARLFRFYRFDRTIKII
                     FFSGEEQGLLGSEAYVDDFPAEMDNIIGVVNLDMFGYDADNDRCFELHVGTRADSNQV
                     GTCFTDVIDNYALDLSYDYVTSGAIPYSDHASFWYAQVGAIEVLENSFYHSQGGCGGI
                     VDENRYYHTVNDRITHMNMPVTLAIAQGGIGTTASLAGTLGRCFAADPVVSAVPQAES
                     VLLSWDPIEGADVYRVYRGTAGCDGSFNLIAETADPVYEDRAISFGTEYVYQVQAAEE
                     GAVCFSQLSNCAATNVPEPPEQVYYYLPFLITAE"
     gene            complement(9107..9181)
                     /locus_tag="PKL60_03555"
     tRNA            complement(9107..9181)
                     /locus_tag="PKL60_03555"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9144..9146),aa:Pro,seq:ggg)
     gene            complement(9322..9687)
                     /locus_tag="PKL60_03560"
     CDS             complement(9322..9687)
                     /locus_tag="PKL60_03560"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNW95245.1"
                     /translation="MLQTYEGLCAFIETKRGCTRDMPFDFDTLCFRVGKKIFVLMSWQ
                     DDPVTINLKCDPEKALKWRSYFPAVTPGYHMNHKHWNTVVCDGSLPDSVLMEMINDSY
                     DLVYASLSKAEKEKLSGEQ"
     gene            complement(9692..10186)
                     /locus_tag="PKL60_03565"
     CDS             complement(9692..10186)
                     /locus_tag="PKL60_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HNW95246.1"
                     /translation="MKIGIMSDTHNKESWLIRALEILRGQGITRLVHCGDLTDFALCR
                     HLRDFQVFYAFGNGDIASGAIALSLQQMNPESRADRALEFTLDGKRFFVIHGNFAEPL
                     NRVLASGDYDYILRGHSHLFEDRWVGRTRLINPGALGDWLKPHSFAVLDVEEDLLIPY
                     ELKE"
     gene            10298..11221
                     /locus_tag="PKL60_03570"
     CDS             10298..11221
                     /locus_tag="PKL60_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95247.1"
                     /translation="MATSLEQGIEAAKSGQMEQALAHLKDAIVEEPTNADVWVWLAAI
                     IEDETKQEIFLKKALELDPSNRPAQRGLAFIERKKHIPPKPGEKLSDYTRPIGIFKAV
                     PAQIQVEPEPEPAPAAEPQIELPAPAAEEAPAPAPAAVEPPKKQTAQKPPKTKVWLDV
                     LMYGVTLMVFIVIGILIGTTLLNVNIPFLTEPTPVLDLLPENEGVFLLENGQYTEMAL
                     NLNVPKDETGIPVTMQALPELVVNNQVISLERVQLLDEAGLPVAFTTRPVKDNIHLLT
                     PQEALAPGRYCIVFTLNVDRNEALYWCLRVE"
     gene            11273..13594
                     /gene="gltA"
                     /locus_tag="PKL60_03575"
     CDS             11273..13594
                     /gene="gltA"
                     /locus_tag="PKL60_03575"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNW95248.1"
                     /translation="MTVESLGLNIPSAHFDDSRHGTIIAKDILAERITRYEVYAPLIA
                     QKARPGQFVILRLTDTGERIPLTIVEHDAQRGSITLIVQAVGKSTEYLESLQVGEQIL
                     DVLGPLGNPSEIKNFDRVVVVGGGVGTAVAYPVAKALKEAGNHVTAIVGARSKDLIIL
                     QKELEQVADELYITTDDGSYGFHGFVTDQLQQLLAGGKTYQYALAVGPLPMMRAISEL
                     TRPYGIPTTVSLNPIMVDGTGMCGGCRVLVGNKVRFTCVEGPEFDGHLVDFDSLSKRN
                     RAYNHLEECRLDEPLTKLESAVEEGKSAPRARQAMPELPVSERIRDFEEVALGFTDAL
                     ALKEAARCLQCKNPKCVAGCPVSVQIPQFIKLILSGEVSAAALLIKQDNSLPGVCGRV
                     CPQTEQCEGACILNKKGVPVAIGALERYVTDYLDAHPVQKAPLDLKPSGHKVALVGSG
                     PACLSCAGDLIKAGHDVTVFEAFHDFGGVLRYGIPEFRLPKVIVTKEVQQLSDLGVKF
                     VPDVLIGATKTVQDLMQIDGYEAVFIGTGAGLPNFLNIPGESLVGIYSANEFLTRVNL
                     MKAYLFPRVDTPILNLQGKRVAVIGGGNTALDSARVALRLGAEEVSIVYRRSETEMPG
                     RFEEIQHAKAEGVRFEFLRNPLEFLGNPDGWLIGMKLIRMELGEPDSSGRRRPIAVAG
                     SEYEVPVDVAVVAIGNGSNPIIQRTTPGLEFNRWGNIIADPDSMETSIPGVYAGGDIV
                     TGGATVILAMGAGRKAAAAINRFLAEKAAQKSS"
     gene            13596..14981
                     /locus_tag="PKL60_03580"
     CDS             13596..14981
                     /locus_tag="PKL60_03580"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95249.1"
                     /translation="MPKIVCLGAGSFSFGLSTLLTLLQSPVLRGSEIALVDINAESLD
                     LTARLAGWLNESWDCGKRISAHSNHKDALPGADYVISAIEVQPRERLWRQDFELTLKY
                     GLRQPYGENGGPGGFAHAARNVNTVLEIARDMEQACPDAWLINFTNPMHRLCYLVNQY
                     SRVKVVGLCHQLAAGYAMAAKALSEHYGFQGAQDFVSTHADPGNHKPMANMAAQGMER
                     FKITAAGLNHFTWMLALQDRKTGENLYPLFRREWQKLPNNFEPLTREIFDIFKVFPIP
                     GDEHLDEYLPWLTNAGQKAWERYDLSLYDWTLYENLRKEQWKQVSEWISSKEQNESLQ
                     NPVSEGAVELIESLSTGRLLSWEALNLPNRGLIDGLPHSAIVEVPGTVSRGGITGQAV
                     GELPRGITELLHRELSASQLCIDAVVHGDKELALQSLLLDPVVDDISSARLILADILE
                     TNKAWLPQFFD"
     gene            15161..15379
                     /locus_tag="PKL60_03585"
     CDS             15161..15379
                     /locus_tag="PKL60_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNW95250.1"
                     /translation="MAEREIGKVKWFDGGKGYGFIARNNGEKDVFVHYSAIIGDGYKT
                     LKENDTVEFSVVQGSKGPQAQDVKIVSE"
     gene            complement(15430..17106)
                     /gene="ettA"
                     /locus_tag="PKL60_03590"
     CDS             complement(15430..17106)
                     /gene="ettA"
                     /locus_tag="PKL60_03590"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNW95251.1"
                     /translation="MPSDTQVIYSMVRVGRIYPPNKQVLRDISLGFYYGAKIGVLGLN
                     GSGKSTLLRIMAGKDLDYLGEIAISKGYSVGMLEQEPQLDESKTVLEVIREGVQPIVD
                     LLAEYDRVNEGFSEPDADFDKLIAEQSRLTDALEHVDAWNLESNLKLAMGALNCPPGD
                     TPVRVLSGGEKRRVALTRLLLTEPDILLLDEPTNHLDAESVAWLEHHLQNYKGTVIAV
                     THDRYFLDNVAGWILELDRGYGIPWKGNYSSWLAQKEERLRVEEKTESKRQRTLQREL
                     EWIHMSPKARQSKGQARVTAYEKLLSQEYEKERKDLEIYIPSGPRLGNVVIEVEGVSK
                     GFGGRQLIEELSISVPPGSIVGVIGPNGAGKSTLIKMIAGVVAPDSGTIRLGETVKLA
                     YADQSRTLDGDLTVFQAITGGADNLQLGDMNVNGRAYCASFNFTGSDQQKPVKNLSGG
                     ERNRVFLARTLTVGANVLLLDEPTNDLDVNTLRALEEALENFAGCAVVVSHDRWFLDR
                     IATHILALGHEGKPRLFLGNWSDFEADQIARYGLDRSGQGRFAYRSLTRN"
     gene            complement(17166..19031)
                     /locus_tag="PKL60_03595"
     CDS             complement(17166..19031)
                     /locus_tag="PKL60_03595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNW95252.1"
                     /translation="MEFPLAWGLLSFASIVLVFQVANLLHGLDSAALILLLQCVLLVI
                     SAALWFLKGKPALYPADLKPGQLLARFKLRGNAALKVLLLLVVGVLLLSLVLIYVVPP
                     NNNDALSFHVARIVRWMQQGTYFPWETPFIWQLTFPVNAQLTYLWTLLLSGTDHFMAY
                     IPFLAGVLTSLLVYAFAREFGYSRRGSAFAGLFWLTFPVVQLHLTSVRHDLISTWLFV
                     SLIYFFHRWGKEKQISALLLSSLALGLVLGTNFSIAAYLPGFAVLALAGFWSYRYSLK
                     QALGWVCAALLAFGLFSSPIYISNQIHFGSPVGPDAAEMTSTAVEAEMPRAKYIVLTS
                     TRWTYQLADLSWLPGSIQTRLTAAKSAAARILTAPLGLNLEGDIATLDAHVFSWDRKY
                     QLQEDEAWFGLVGFLLIIPTSLVSVVQAVRKKEALRAFPFVFYLTALVTCSLIRPGWT
                     PFDGRYFMPAVALAAALLPMWFEGKRPRVWVQWILVFTALFSSAMVILVNPAKQIVGG
                     AAVWNMNRIDRLTRQSYSSKEMLYLAEQIPSGAVVGVAAEANDYQEYGLFGADFSRRV
                     VNVYPVSLAADKAWLGERGVQYLLVRASGAGSPEVAQNFQAGESLGDWILYERKP"
     gene            19259..20218
                     /locus_tag="PKL60_03600"
     CDS             19259..20218
                     /locus_tag="PKL60_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95253.1"
                     /translation="MPTTIQIKLRFNLLLCAFAAVLSACSPAIQTPVPSQTPLPPTLT
                     PTETTTPTQTPTPAPLKLDIPVLLEQCETLSITNTEVIVSGHLYLPEFRIYGYSGWKG
                     MNLKQFLPNDQKGITALIPLGDGPNTMDELPVYFTPRDLRARSDSGQLILHGHSVSIQ
                     GRAEYVVSEGVPRCQVWVEKIESLMPAELLIPQTFEISNLLKEQNIGSKSHPEIVSTC
                     ELMGRKQQMVSIKGSVIAESGPDLCKQEICRFKLTDKTGTVTALFLLADTPNSINFIS
                     NQGWKLMDQDGIPRDLLNLVFTGTLFVDPSDGCSLLVYQIKEH"
BASE COUNT         4743 a         5846 c         5505 g         4238 t
ORIGIN      
        1 tgaaatcgtg gaagccatgc aagcgccgct ggaggaaatt gccgcaaccg ccaaacgtgt
       61 gctggagaaa accccgcccg aattgatttc cgatatcatc gaccgcgggg ttgctttgtg
      121 cggcgggacc gcgctgatga aagggttgga caaatacctc actgttgccc tgggcattcc
      181 cgcatacatc gtggataacc cggtcacctg cacagtggag ggaaccgcca aagccttcgg
      241 agtgctcgac ttgatactcc gcagccaaca aaactgagcc tgccgtggaa gcctcacaag
      301 cccccgaact acaaccagat gccgttttca cggacaaaaa gaagcgccgc atccgcgcat
      361 ggcgttttgt tttcagcctg ctgctgcttg ccggtctgtg cgcctcagga tatcatgcct
      421 ggcagtggtt tgcgccgcgc gccgccagat atgggctgtc catcctgtgg atcaatggaa
      481 agctcatcct gatcttgctg ccggtagtgt tggtcttgct gttctggctg cttttcgcca
      541 atttgccgcg caagaaaacg ataatttcct tatcagaaga tggaatccgc aaaaagaacg
      601 gaagacggga cctgaagctg cgctgggact ctttggaaac gctgcggttc aagttttccc
      661 gggctttctt cctgggaatt ccaggcagcc ggcgtgaacg cgtggagtta acggacgcag
      721 tcgggaacaa attgtgcttc gacgcgcgca tggaccgctt tgaggagctt gtcaatcgga
      781 tacgcgagcg ctcttttccg gcgttatacc aactttctca ggaacggctt tcccgcgcgg
      841 gtaagcttgc attcgggaag caggtgagcc tttcaccgga cagcttgcaa atacgaaaaa
      901 cctccctgcc ccttactcag ataaaatcgg cagcgatcga aaagggctgt ctgcgaatca
      961 aaacaataaa caacagaaaa aaacgcatcc gggtggataa actggataat ccggatgtcc
     1021 tgctgcactt attggggaaa tcaacctaat cttccgcttc cagataaaaa gtcagtgtct
     1081 gcaatttgac caccgggtca atattgatga catgcacgct ctcgggcact ttcaacacca
     1141 gcggcgcgta attcaagatt gcccgaatac cgccggaaac cagctggtct gttacttgct
     1201 gcgcgcatcc cgccgggact gtgagcacag caacctgaat attaaggcgc ttcaggtcat
     1261 ccgccaggtt gcgcacgtcg ttgatctgca ttccgctttg cgtgctgcca attaagtcag
     1321 ggttgttgtc acacgccagc acgatttcaa aacccctgcg ggcgaaaccc tggtatctca
     1381 acagcgcctg accaaggttc ccgacgccca cgatcaccac gggccaaatc tggttgagat
     1441 gcaaaatcgc gcgcagattc tcgatcaggc tgatgacatg ataaccgctg ccttgcttgc
     1501 caaaaccgcc gaaatacgag aggtctttac gaatttgggc tgcggaaaca tccaccatac
     1561 gcccaagctc cgcagaagac accacgcccc ggccttccct cagcaggtgg ttcagcgcct
     1621 gcagataaac tggaagccgc tcgactacaa tgtcgggtat ttttgctttc tccatccgtc
     1681 ctgacctttg ctttctctcg tccagccgac aagtaagaaa aaagatgtta tcatcacaca
     1741 agataggtat attatattag gttatgaatc caatttgcgt ttaatatttc cagcattgtg
     1801 aaaatgtcca caattactac cagttgaagg agttgacagg atgaaagcag ccagaaccat
     1861 tctctacatc gtcgcgggga tactgatcgt gttgtcgttc ctgttcattc tcggcgcgtt
     1921 cagcccatcc ggaagcacaa gctggctgac ggtcgggctt attggcctgg cgattgcgtt
     1981 cgtattcatc attatcggcg cgcgcatcgg cgcgaaagca gccggcacga cgcagaacat
     2041 ctccgtaaac gtggatttgc ccggtaatgt aaagatggac gccatcaaat gccagtcctg
     2101 cggctcaccg ctggcagccg acgatatcaa aatgattcag ggcgcgccgg ttgtaacctg
     2161 ccacagctgc ggaactacct accaattgac ggaagaacca aagtggtaaa aagtatgaaa
     2221 aaactaaaca cagtctttct tctcctggtg cttttggctg cctttatgtt tccggcagcg
     2281 gtttccgcac aaacttaccg ctttacggta agcgagtatg aagtggaagc ctacctggaa
     2341 caagacggca gcctgacctt gcggtacctg atggtttttg agaacgatcc ttccgcggat
     2401 cccattgatt tcatcgacct tggtttgccg cttgctcaat acgatcttca taacatcacg
     2461 ggcacaatca atggagaaac catccccagg attggcgatt cggcctacgt aaacggggcg
     2521 gaactcgcac tgcaagacct cgctattcag ccgggcgaga cgggcacagt catcgccact
     2581 gtaacaggca tcaccggtgt gctcttcccc tacgaccagg gcgaccgcga aaactacatc
     2641 aatttccagt tctcccccaa cacatttgac gcggagttcg accgttctac cgcgaccaag
     2701 tatcgcatga ctatcgttct cccgcccggc gttggcatcg atgccggcgt gtattaccgt
     2761 ccttccggtt ggccggggag cgataccccc gaagcctcca cgacccagga tggacgcgta
     2821 tactactcct ggtacaccga aaacgcgaat cttcacagca attattactt cggcactgcc
     2881 tttcctgcct ctgctgtccc aactggggca gtcagcagca aagaagaata tacccagcag
     2941 ggaggggaaa catcatcggg ttccggaggt ttttccattg acccgggcac gatattctgc
     3001 tgcggcggca gtctgatttt tggcgcgttt gtcgtcctcg gaatcatcaa aggctccaag
     3061 gcggaaagca aacgcaagat ggcgtatatg tcccccaaga tttctatcga agggcagggt
     3121 gttaagcgcg gattgaccgc ggtggaagcc ggcatcctca tggaagaacc gctcgacaaa
     3181 atcctgacca tgatcatgtt cgggctgctg aagaaagaag ccatcaccat cacccaaaaa
     3241 gacccgctta ccatcaagcc gagcgaaccg ctgcccgacg gcctgtatga ctatgaaaaa
     3301 ggcttcattc aggcgttcct gaaggagaaa ccgtcggaga gaaaacaagc cctgcaaacc
     3361 atgatcattg acctggtaaa cagcgtcacc gcgaaaatga aaggtttcag caaggcggaa
     3421 acagttgctt actacaaaga tatcatcgcc cgcgcctgga aagccgttca ggacgcgaac
     3481 acgcctgaga ttaagagcgc tcaatacgac cacacgctgg aatggacgat gctcgataaa
     3541 gacttcaaca cgaaaaccga agaaaccttc tccggtggtc cggtcatcgt accgatgtgg
     3601 tggccgcgtt acaaccccgg ttggcagccc tccgcgcaga ccagcggcgg cggcggactt
     3661 aacctacccg ccagcgctgg taccggcgcc ccatccggcg gaggcagcaa accctccctc
     3721 tccctgccga atatccccgg ctctgatttt gccgcttcaa tgataaacgg cgcttccagc
     3781 ctggctgccg gagcaattgg cgacctgacc ggttttaccg gcgcgattac caacaaaacc
     3841 aacccgatcc cgaccaccag ctacaaaagc ggcagcggcg gttttcgtgg aggcggcggc
     3901 ggcgggcact cctgtgcgtg cgcttgtgct tgcgccggct gcgcctgcgc ttgcgctggg
     3961 ggcgggcgct gacactgaaa aggcatatgg cacatacact tctccccaaa aaaccgctcc
     4021 cacctgcggc tcccggtttg taccagttcg atatcgcgga tgacgattcg aaatcccggg
     4081 tgcatatccg cctggacgaa gacggcagcg gtctgctggt tgtgaacgcc agccgggtcg
     4141 tacacctgaa cccgaccgcg gttcagatgg catactatca tctcagtaaa gccggagaga
     4201 aagatgcagt ccgcgcgctc agcaagcttt acaaagcgcc gaaaaacagg atttctgagg
     4261 attatgccag gatttccaat caggtggacg ctctgttgga tgaacaaagc ggtttatgcc
     4321 cggtatgcga tttgggtctg gagaagcaca tgcctttttc ggttgcgctg tccgcgccat
     4381 accgcatgga cctggcacta acctaccgct gcaacaacag ctgcgcccac tgttacaacg
     4441 cccgcccgcg cagctacccg gaactggata ccaggacctg gaaggaaata ttggaccgcg
     4501 tctgggaact gcgcattccg catgtggttt tcaccggcgg cgagcccacg ctgcgggaag
     4561 acctggtcga actcgtcgcc tacgcgcagg aactcgggct ggtgacaggt ctgaacacca
     4621 acgccaggcg cctcgccgat gagcagctta tgcaggattt ggttgccgca gggttggacc
     4681 acgtgcaaat aaccgttgaa tcccacaacc ctgccgtgca taacgccatg gtacgggcag
     4741 ccggggcatg ggatgaaacc gtcgccggcc tgcgcaatgc ggtccactcc ggcatctacc
     4801 tgatgaccaa taccacgctc ttgcgcaata acacatcgga tctcgaggag atcctcaact
     4861 ttttggccga tgaaaaagta ccgaccgtcg gcttgaacgc gctgatttac tccggacacg
     4921 gcgcgacggt caacaccggc ctggcggagg tggaattgcc gcccttgttg gagctggcca
     4981 cacggcttac ccgccagcat gaccagcgct tgatttggta caccccgacc cagtactgcc
     5041 acttcaatcc catcctgctc agcctgggga tcaaaggctg cacggccgcg ctctacaaca
     5101 tgtgcgtgga accggacgga caggtcatcc cgtgccagtc ctactacgaa gcgctcgggc
     5161 atatcctgga tgacgactgg gacaggatct ggaatcatcc gctctcgcgc gaattacggg
     5221 agcgaaaaaa tattcccgag ggctgccagc aatgcgactt tttggtggaa tgcggcggcg
     5281 gctgccccct ggcgcgcgcg catcaatccg ttcaagcgat cacagcagac atcttttagt
     5341 ggaggcccaa tgattgactt catcaagcgc ttattctcca aagaacccgc acaccttccc
     5401 ctggaacccg gcttccacgc ataccacagc cccaaggacg acccgcaacc ttaccgcttg
     5461 cacctgagaa tcgacccgga cggtgaaggg ggcctaattg tgaacgcgtc ctccgtgctg
     5521 cacctgaacc agacagcagc cgaattcgcg tattacctca tcaagggcaa acaggatgcg
     5581 gaaattaccc ggctggtctc cgaacgtttt tccgccccga tcgaaaccat cgccgcagat
     5641 gtggtctctt tccatcagca gctgctctcg ttcatccgga agacagacca ggagcccatt
     5701 accagctttg gctttgaacc gcatacccaa ttaaaagaca tctccgcacc ataccgactg
     5761 gactgctgcc tgacaaaatc ctgtgagacg ccttccgagc ccgcgcttct gagcgacgaa
     5821 ttggacagct cggcatggaa aaagatgatc cgcaaagcct ttgaggctgg cattccgcac
     5881 ctgattttct ggggcggcga gcctactttg cgcgctgacc tgcctgaatt gctggcttac
     5941 tgcgaggagc tggggctggt aaccgggctg gtgagccgcg gaagcaagct gaacgatgcc
     6001 gcgtatgttc aatcattgat tgacagcggg cttgaccatc ttttcgttcc tttccagccc
     6061 ggcgacgact ctctgctctc agccctgcgg caaatcctcc cgctggacct gtacacctgc
     6121 gtcggtctgc tgcttcatgc ggatactgaa agccgccaga cgattgaaga gctcagagtc
     6181 ctgggcgtga acgcctttgc gcttatccca ctgagcacag acgcggctcc ggcaatcgat
     6241 cgagcggaag aggctgtgct ggaaagcgga cttccgatga tcaacgacct gcctctgcct
     6301 ttggacctgc gcgctgcgct ccctgagcaa aacttcacgg ctgcgcctga cccggaagcg
     6361 gaatttgtta cgctggaact tgccccgaat ggcgacctga ggacgctgcc catgtatcac
     6421 aaatacctcg gaaacctgct gaccgatagt tggcgcgacc tctggcagca gcgctaccag
     6481 gcataacatg aatattcaca cctggcactc ccgctatgtt ctgcaagcac tttggacggc
     6541 aaaactgcgc cagttcctgc tgaacaaggt taacgcccga cccggggagc agatcctgga
     6601 ggttggcagt ggtacgggcg ccttgttccc ggattttgag aaccgcggat tgcgcgtggt
     6661 cggtctggat tacaaaccca aacgatgcca atattcgctt gcgtataaca gtcagcgcac
     6721 ggtcatctgc gggaacgcct acgccatccc agccgctgac caaagctttg acctgtgcgt
     6781 atgtcattac ttgctgctct ggttgaagga accggagcgc gcgctggctg agatggcgcg
     6841 agtcaccagg cgcggcaggc atgtcatcgc tttcgcggag ccggactacc aatcccgtat
     6901 cgattacccg gaagttttcc agaaaattgg cgaagtgcag aaccgcagcc tggaatttca
     6961 gggtgtggac ctgagcatcg gccgacgcat gggggcgatg ttccgcggtc tgggtctgca
     7021 aaatgtaaac gtggggctgc tggcgggcga gtggaaagag cccgatccgc aggtctttga
     7081 agcggaatgg gatatcatcg cttacgattt aggcgggctg gcgccagtgg atgaaatttt
     7141 ggctttcaag gccaaagcgc gcgaaacctg gctgagcggc caggcaaccg tctttatccc
     7201 cacgttctat gcgtatggga tggtgtaaac agcacctgat ttaataatac agcttgcagg
     7261 agaaggcgca tgaaaaagac aaaactgggt ttgctgcttg tatctcttat tttgctcttt
     7321 tcagcgcctc tcagctcagc cgcgggtcaa acccagacct gccccactaa ttccagcgtg
     7381 gcggaaattc tcaatcaagt ggacagcgcg tatatcacca aatggatccg agacttcagc
     7441 ggcgaagatt ttgtcgcaat cggaggcgcc caacgcagaa tacttacccg tcagtctctg
     7501 caattgttca atttcaatgc caacgccctg gcttatccct accttggcga gttgctgcgg
     7561 agcttcggat acgaagacgg cacgtccctg gctgatcacc tctacacgcc aaccttgaag
     7621 ttaacctctg agggaatacc cagccagata ttcgatgagc gcggcagacc gatagtactg
     7681 attccagagc tgatggatga acaggcgcag gctgccagct ggaaaaacaa gatagtcatc
     7741 ctcccggggc acggcgcgca tgccgatgag ctggtgctca tgaccgccca tatggacagc
     7801 acttcgggtt cggcctctac gaacgcgccg ggggctgagg acaatgccag cggtatcgcc
     7861 gcgctgatgg aagccgcccg cctgttccgt ttttacaggt tcgaccggac catcaaaatc
     7921 atcttcttca gtggcgagga acagggtttg ctgggcagtg aagcctatgt ggatgacttc
     7981 cctgcggaaa tggacaacat tatcggggtc gtgaacctgg acatgtttgg ctatgatgcc
     8041 gataacgacc gctgtttcga attgcacgtg gggacgcggg cggactcgaa ccaggtcgga
     8101 acctgtttta ctgatgtaat tgataactac gcgttggacc tgagctacga ttatgtcacc
     8161 agcggagcta tcccctattc tgaccatgct tccttctggt acgcgcaggt tggggcaatt
     8221 gaggtgctgg aaaattcctt ctatcacagc cagggcggct gcggcggcat cgtggatgaa
     8281 aacaggtact accacaccgt caatgacaga atcacccaca tgaatatgcc tgtgaccctc
     8341 gccatcgcgc aaggcggtat cgggacgact gccagcctgg ctgggacgct gggcaggtgc
     8401 ttcgcggcgg accccgtggt ttctgctgtt ccacaggctg agtcggtcct gctgagctgg
     8461 gacccgattg agggagcgga tgtttaccgg gtgtatcgcg gcaccgctgg ttgcgacggc
     8521 agcttcaacc tgattgccga aactgccgat cctgtatatg aagaccgcgc aatcagtttc
     8581 gggacggagt atgtttacca ggtccaggca gctgaggagg gcgcggtgtg cttttcgcag
     8641 ctttccaact gcgccgcgac caacgtgccc gaaccgccgg agcaggttta ttactacctg
     8701 ccgttcttaa ttaccgccga gtaagcagag aaatgaccga tctgctgaaa ataccagaag
     8761 gtcttcttta ttttcgccag ccttctattg ctctcgccgg tctccagacc gagcgagggg
     8821 cttgaccgca gatctccaat caatatacca accggacgta ggggcgaagc atccagaatc
     8881 acgcaatcca taacgcgaag ttggcggctt aaaaacgcaa gccgacctgt tccacgctgg
     8941 tcaggagacc agcgcgatcg gggatttttc catgctctcg aacatggttg cgatatggag
     9001 accaaaaggt cagatgcctt acatggtctc cggaccatgc aagaacaggc acggcaaaga
     9061 accaataaaa agaccctttg gatttctcca aaaggtcttc actctgtcgg ggcgccggga
     9121 ttcgaacccg ggacctcttg caccccatgc aagcacgcta gccggactgc gctacgcccc
     9181 gaacaacagg cgttgattat actcaagccc cgcggaaagt gcaagctttt tttacccccg
     9241 ctttttttac ccccggcttt cccccttttc cccgcgctcc cctgcagtat ttatttctca
     9301 cccgggcttt cctttgtttt tctactgctc cccagaaagc ttttctttct ctgccttgct
     9361 caggctggcg tacaccagat cgtagctgtc attgatcatc tccatcagca cggaatccgg
     9421 cagcgagccg tcgcatacca cggtattcca atgcttatgg ttcatatggt agccgggcgt
     9481 gaccgccggg aagtaactgc gccatttcaa ggctttttcc gggtcgcact tcaggttgat
     9541 ggtcaccggg tcgtcctgcc agctcatcag gacgaaaatt ttttttccaa cccgaaagca
     9601 cagtgtgtca aaatcaaaag gcatatcgcg ggtgcagcca cgcttggttt cgataaacgc
     9661 gcacaatcct tcgtaagttt gcagcatttc ctcattcctt cagctcgtac gggattagca
     9721 ggtcctcttc gacatccagg accgcaaaac tgtgcggctt cagccagtcc cccaacgcgc
     9781 cagggttaat caagcgcgtg cgcccaaccc accggtcctc gaacaggtgc gaatgtcctc
     9841 gcaggatata atcataatcc ccgctcgcga gcacgcgatt aagcggttcc gcgaagttcc
     9901 cgtgaatgac gaaaaagcgt ttgccgtcca gcgtgaactc cagggctcta tctgcccggc
     9961 tttcggggtt catctgctgc aggctgagcg cgatcgcgcc gctggcgata tcgccgttgc
    10021 caaaggcgta aaaaacctga aaatcccgta aatgtcggca cagcgcaaaa tccgtaaggt
    10081 cgccgcagtg tacgagccgc gtgatgccct ggccgcgcag gatttcaagc gccctgatca
    10141 gccaggactc cttattatgc gtatcgctca tgatgccgat tttcacttct aactccctga
    10201 ttcatccggt aatttatcca ccacaattgt atctattgat tctcatatgg taaaatcgaa
    10261 ttgcattaat acaggacgat caggcggagc tggcattatg gcaacatccc tcgaacaagg
    10321 cattgaagcg gcaaaatccg ggcaaatgga acaagccctg gcacacctga aggatgccat
    10381 tgtggaagaa ccgaccaacg cggatgtgtg ggtttggctg gctgccatca tcgaagacga
    10441 gaccaaacag gagatttttc tcaaaaaagc gctcgaactg gacccctcca accgtcctgc
    10501 gcagcgcggg ttggcgttca tcgaacgcaa aaagcatatc ccccccaaac ccggcgagaa
    10561 actttccgat tacacccgtc cgattggcat cttcaaagcc gttcccgccc aaatccaggt
    10621 ggaaccggag cctgaacccg cacctgcggc cgaaccgcaa attgagttgc ccgcgcccgc
    10681 cgctgaagaa gcgcctgccc cggcgccagc tgcggtggaa ccgcccaaaa aacagacagc
    10741 ccaaaaaccg cccaagacca aggtctggtt ggatgtattg atgtacggcg tgacgctgat
    10801 ggtattcatt gtcatcggca ttctgattgg caccacgctc ttgaacgtaa atatcccctt
    10861 tctgacggaa cccacgccgg tcctggacct gctgccggaa aacgaaggcg ttttcctgct
    10921 cgaaaacggg cagtacaccg agatggcgct gaacctgaac gtcccgaagg acgaaacagg
    10981 cattccggtg accatgcaag ccctgccgga attggtggta aacaaccagg tcatcagtct
    11041 ggaacgcgtg cagctgctgg acgaagccgg cttgcccgtg gcctttacca cccggcctgt
    11101 gaaagataat atccacctgc taacccccca ggaagccctg gcacccggga gatactgcat
    11161 tgtttttacc ctgaatgttg accgcaacga agccctgtat tggtgcttgc gcgtggagta
    11221 aagaacgctt tgcccttaca acgacatacc tatcccccgg aggctgattt caatgacagt
    11281 tgaatccttg ggattaaaca tcccatctgc ccacttcgat gattccaggc acggcacaat
    11341 catcgccaaa gacatcctgg ccgagcgtat tacccgctac gaagtttacg cgccgctgat
    11401 agctcaaaaa gccaggccgg gtcaattcgt aatcctacgg ctgacagaca ccggtgagcg
    11461 cattccgctc acaatcgtag agcacgacgc gcagcgcggc agcatcacgc tgattgtgca
    11521 ggctgtcggc aaaagcacgg agtatctcga gagcttgcag gttggcgaac aaatcctgga
    11581 tgtgcttggt cctctgggca acccctcgga aattaagaat ttcgatcgtg ttgtcgtggt
    11641 cggcggcggc gtcggcacgg cggtagccta cccggtcgcg aaggcgctta aagaagccgg
    11701 caatcacgtt accgcaattg tcggagcgcg cagcaaggac ctgattatcc tgcagaaaga
    11761 gctggaacag gtggcggacg agctgtatat caccacggac gacggctcct acggcttcca
    11821 cggtttcgta accgaccaac tgcagcagct gttggctggc ggaaaaactt accagtacgc
    11881 gctggcggtt ggtccgctgc caatgatgcg cgccatttcc gagctcaccc ggccgtacgg
    11941 catccccact accgtcagcc tgaaccccat catggtggac ggaacaggca tgtgcggcgg
    12001 ctgccgcgtg ctggtgggga ataaggtgcg tttcacctgc gtggaagggc cggaattcga
    12061 cggccacctg gtggacttcg actcgctgag caagcgcaac cgcgcctaca accaccttga
    12121 ggagtgccgc ctggacgagc cgcttacaaa gctggaaagc gctgtggaag aaggcaaaag
    12181 cgcgccgcga gccaggcagg ccatgcccga actgccggta agcgagcgca tccgggattt
    12241 cgaggaggtt gccctcgggt ttactgacgc gctcgcgctc aaggaagccg cccgctgtct
    12301 gcaatgcaag aaccctaaat gcgtggctgg atgcccggtc tccgtccaaa tcccgcaatt
    12361 catcaagcta atcctttccg gcgaagtgtc cgcagcagcg ctgctcatca aacaggacaa
    12421 ctccctgcct ggggtgtgcg ggcgtgtatg tccgcagacg gaacagtgcg aaggcgcctg
    12481 cattctgaac aaaaaaggcg taccggtggc gattggtgcg ctggaacgct atgtcaccga
    12541 ttacctcgac gcgcatccgg tccaaaaagc gccgctggat ttgaaaccaa gcgggcacaa
    12601 agtcgcgttg gtcggcagtg gaccggcatg cctgagctgc gcgggggact taatcaaggc
    12661 cggccacgat gtaactgtct tcgaagcctt ccacgatttt ggcggagtcc tgcgctacgg
    12721 catccccgaa ttccgcctgc ccaaggttat tgtcacgaaa gaagtgcagc agctttcgga
    12781 cctgggcgtg aaatttgttc cggacgtgct gattggcgct accaaaaccg tccaggattt
    12841 gatgcagata gacggctacg aggcggtgtt tatcggcacc ggcgcgggac tgcccaactt
    12901 cctgaatatt cccggcgaga gcctggtggg gatttactcc gccaatgaat tcctgacccg
    12961 cgtaaacctg atgaaagcct acctcttccc gcgggtggac acacccattc tcaacttgca
    13021 aggaaaacgc gtggctgtca tcggcggcgg gaataccgct ttggattccg cccgggttgc
    13081 cctgcggctt ggcgctgaag aggtttccat tgtttacagg cgcagcgaaa ccgaaatgcc
    13141 agggcgcttt gaggaaatcc agcacgcgaa ggccgaaggc gtgcgcttcg aatttctgcg
    13201 taatccgctc gagttcctgg gcaacccgga cggctggctg atcgggatga aactcatccg
    13261 gatggaactt ggcgagccag attccagcgg cagaaggcgt ccgatagcgg ttgcgggctc
    13321 agaatacgaa gtgccggtgg atgttgcggt ggtagccatc ggaaacggct ccaacccgat
    13381 catccagcgc accactccag gtctcgaatt caaccgctgg ggcaatatta tcgctgaccc
    13441 tgacagcatg gagacttcca tcccgggtgt gtatgccggc ggagatattg ttaccggcgg
    13501 cgcaacggta attctggcga tgggagccgg ccgtaaggct gccgcagcca taaaccgctt
    13561 tctggccgaa aaagccgccc aaaaaagcag ttgatatgcc aaaaatcgta tgtttaggcg
    13621 caggtagttt ttcgtttgga ttaagcaccc ttttaaccct gttgcaaagc ccggtgctgc
    13681 gcggaagtga aatcgcgctg gtggatatca acgcggaatc cctcgacctg acagcccgtc
    13741 tggccggctg gttaaatgaa agttgggatt gcggcaagag gatcagcgcg cattccaacc
    13801 acaaagacgc cctccccggc gcggattatg tcatcagcgc gatcgaagtg cagccgcgcg
    13861 aaagattgtg gcggcaggac ttcgagctga ccctgaagta tgggctgcgc caaccatacg
    13921 gcgaaaacgg cggacccggg ggattcgcgc atgccgcccg caacgttaat actgtgctgg
    13981 aaatcgcccg ggacatggaa caagcttgcc cggatgcctg gctgataaac ttcaccaacc
    14041 ccatgcatcg gctctgctac ctggtaaacc agtactcacg cgtgaaggtg gttggcttat
    14101 gccaccagct tgcggcgggt tacgccatgg cagccaaagc cttgtctgag cattacggct
    14161 tccagggcgc gcaagacttc gtaagcacgc atgccgaccc cggcaaccat aagcccatgg
    14221 cgaacatggc agcccagggc atggagcgct tcaaaatcac ggctgccggc ctgaaccatt
    14281 tcacctggat gctcgccctg caggaccgga aaactggcga gaacctctac ccgcttttcc
    14341 gacgggaatg gcagaagctc ccgaacaact ttgagccgct gaccagggaa atattcgata
    14401 ttttcaaggt atttccgatt ccgggcgatg agcacctgga tgagtatctg ccctggctga
    14461 cgaacgccgg gcaaaaagcc tgggaacgtt acgacttgtc tttgtatgat tggaccctgt
    14521 atgagaacct gcgcaaggag cagtggaagc aggtttctga gtggatttcg agcaaggaac
    14581 aaaacgaaag tctgcagaac cccgtcagtg aaggagctgt ggaactcatc gaaagtttgt
    14641 caacaggccg gctgctttcc tgggaagcgc tcaatcttcc caaccgcggc ctgattgacg
    14701 gactgccgca cagcgcgatt gtggaagttc ccggaacggt gtcgcgcggc gggattaccg
    14761 gtcaggctgt tggcgagctt ccgcgtggaa ttaccgagct gctgcaccgc gagctgagcg
    14821 caagccaact gtgcatcgac gcggtcgtgc acggggataa agaactcgcg ctgcaaagtt
    14881 tgctgctgga cccggtcgtg gacgatatca gcagcgcccg cctgattttg gctgatattc
    14941 tggagaccaa caaagcctgg ctgccccaat tttttgatta gctgcgcgga aagaatcagg
    15001 atacttaccc agaggcaaaa ggtcagaatt gacgccgcgc ctttgttgta gtataaaaac
    15061 aaattgttac ggtatattca gatgcctgca taacaatttc atttgaagga ccggaaacac
    15121 atgtttacag tttttttcaa tcacggagga ttagagaatt atggcagaac gggaaattgg
    15181 taaggttaag tggtttgacg gcggtaaagg ctatggtttt atcgcgcgca ataacggcga
    15241 aaaagacgtt tttgttcact acagcgccat tatcggtgat gggtacaaaa ccctcaaaga
    15301 aaacgacact gtcgaattca gcgtggtcca ggggtcgaaa ggtccccagg ctcaggatgt
    15361 gaaaatcgtc tctgagtaat tgataaaaga atcgaagacc ggtctggtta tgcagaccgg
    15421 tttttttgtt taattccggg tcaaactgcg ataggcaaaa cgcccctgtc ccgaacggtc
    15481 caggccgtag cgcgcaatct ggtccgcttc aaaatcggac caattgccga ggaaaagcct
    15541 gggtttcccc tcatgcccca gcgccaggat gtgcgtcgca atccggtcca ggaaccagcg
    15601 gtcgtggctg acaaccaccg cgcagccggc aaaattctca agcgcttctt ccagcgcccg
    15661 caaagtgttt acatccaggt cattggtggg ttcgtccagc agcaggacgt tcgcgccgac
    15721 ggtcagcgtg cgggcaagga aaacccggtt gcgctccccg ccagaaagat ttttgaccgg
    15781 cttctgctgg tcagagccgg taaagttgaa cgaagcgcag taagcccgtc cgttaacatt
    15841 catatcgccc aactgcaggt tatccgcccc gccggtgatt gcctggaata cggtcaggtc
    15901 gccgtcaagc gtgcgcgatt ggtcggcgta ggccagcttg acggtctcgc ccaggcgaat
    15961 tgtgcctgaa tccggcgcca ccacaccggc aatcatttta atcagggtgg actttccggc
    16021 tccgttgggg ccgatgaccc ccacaataga acctggcggt acgctgatgc ttaattcctc
    16081 gataagctgt cggccgccga agcctttgct gacgccctcc acttcgatga ccacattgcc
    16141 caggcgcggc ccggagggaa tatagatttc caaatcctta cgctcttttt catattcctg
    16201 ggagagcaat ttttcgtagg cggttacacg cgcctgcccc ttggattggc gcgcctttgg
    16261 agacatatga atccactcca actcgcgctg taaggtgcgc tgtcgcttgg attccgtctt
    16321 ttcttctacg cgcaagcgtt cctctttttg ggccaaccaa ctcgaatagt tgcctttcca
    16381 gggaatgccg taaccccggt ccaattccag aatccaacct gccacgttat cgagaaaata
    16441 ccggtcatgt gtcacggcga tgacggtgcc cttgtaattc tgcaggtggt gttccagcca
    16501 ggctacggat tcggcatcca gatggttggt cggctcatcc agcagcagaa tgtccggctc
    16561 ggtcaagagc aggcgggtca gcgcaacgcg ccgtttttcg cctccggaaa gtaccctgac
    16621 aggcgtatcc ccgggcgggc agttcagcgc tcccatcgcc agcttcagat tgctctcgag
    16681 gttccaggca tccacgtgct ccagcgcgtc cgtcaggcgc gactgttcgg cgatgagctt
    16741 gtcaaagtcg gcgtcaggct cagaaaagcc ctcgttcacc cggtcatatt ccgccagcag
    16801 gtcaacaatc ggctgcacgc cttcgcgaat cacttccagg acagtcttgg actcgtccag
    16861 ctgcggctcc tgctccagca tgcccacgct gtatcccttg gaaatggcga tttcgccgag
    16921 ataatccagg tccttgcccg ccatgatgcg cagcagcgtg gatttacccg agccgttcag
    16981 gcccagaacg ccaattttcg cgccgtaata gaacccgagc gagatatccc gcaaaacctg
    17041 cttgttgggc ggataaatcc ggcccacacg gaccatggaa taaatgacct gggtatcaga
    17101 aggcatatgt gctccttaat gctatttttc ctggcagtgt tggtacaaaa ccggagaaat
    17161 ttttctcatg gcttgcgctc gtaaagaatc cagtccccca ggctctcccc tgcctggaaa
    17221 ttctgcgcga cttccgggct gcctgcgccg gacgcgcgca ccagcagata ttgcacgccg
    17281 cgctcgccca accaggcttt atccgccgcc aggctgacgg ggtaaacatt caccacccgg
    17341 cgcgaaaaat ccgcgccaaa cagcccgtac tcctgataat cgttcgcctc agcggccaca
    17401 ccgaccactg ccccggaggg tatttgctcc gccaggtaga gcatctcctt ggaggaatag
    17461 gattgacgcg tcagtctatc gatgcggttc atgttccaga cggctgcgcc tccgacaatt
    17521 tgctttgcag ggttgaccaa aatgaccatc gccgagctga acaacgccgt aaacaccaaa
    17581 atccactgca cccacacgcg cgggcgcttg ccctcgaacc acattggcag cagcgcagct
    17641 gccagcgcca cggccggcat aaaatacctg ccgtcaaaag gcgtccaacc cggccggatg
    17701 aggctgcagg tgaccagcgc ggtcaggtaa aacacgaatg ggaatgcccg cagcgcttct
    17761 tttttccgca cagcctgaac aacgctgaca aggctggtag ggataattaa caggaaaccc
    17821 acgagtccaa accaggcctc atcctcctgg agttggtact ttctatccca gctgaaaaca
    17881 tgcgcgtcca gtgtggcaat atcgccttcc aggttcaggc ccaaaggcgc agtcagtata
    17941 cgggcagccg cagatttcgc agcagtcagc cgggtctgaa tcgagcccgg cagccaggaa
    18001 aggtccgcca attgataggt ccagcgtgtg ctggtgagca cgatatactt tgcccgcggc
    18061 atttctgctt ccacagccgt actggtcatc tcggctgcgt ccggacccac cggcgagcca
    18121 aagtgaatct gattgctgat gtaaatcggc gagctgaaca agccaaaagc cagcagtgcc
    18181 gcgcaaaccc acccaagcgc ctgtttgagc gaatagcggt aactccagaa tccagccagc
    18241 gcgagtacag cgaagccagg caggtacgca gcaatcgaga aattggtccc cagcaccagc
    18301 ccaagcgcca aactggatag cagcagggcg ctgatttgtt tttcttttcc ccagcggtga
    18361 aagaagtaaa tcagggatac aaacagccag gtactgatca ggtcatgccg gacagacgtg
    18421 agatgcagct gaactacggg gaaagtgagc cagaagagcc cggcaaaagc cgagccgcgc
    18481 cgggaatagc cgaactctcg cgcgaaggca tagaccaata aactggtcag cacgccggca
    18541 aggaaaggaa tatacgccat gaagtggtct gtcccgctca ggagcaaggt ccacaggtaa
    18601 gtcagctggg cattgaccgg aaaggtgagc tgccagatga agggcgtttc ccacgggaaa
    18661 taagtgccct gctgcatcca gcgtacgatg cgggcgacgt ggaaagaaag cgcgtcgttg
    18721 ttatttggtg gaacgacgta gataagcacc aacgaaagca gcagcacccc caccaccaaa
    18781 agcaagagca ctttcagcgc ggcgtttccc cgcagcttga accgggctaa cagctgcccg
    18841 ggtttcaggt cagccggata gagcgcgggt ttgcctttca ggaaccataa agcagcgctg
    18901 attacgagaa gcacgcattg cagcaggagg attagagcag cggaatccaa gccgtgcagc
    18961 aggttagcga cctgaaaaac aagcacgatg gacgcaaagc tgagcaggcc ccaggccaac
    19021 ggaaactcca aagctcgcct taagcgcagc agcgccgtaa gaaaaagcgt actaaagacc
    19081 aacatgagga ttgacagcaa gaaaatcaag gcagagctcc cctattagtt taccccaagc
    19141 gcttaggctt gggtattcca taacgacctc ttgcagttag aaattcctat tgcttttttt
    19201 gtttccagga tattgcccct ataataaagg cagattggtc accgtccaag gaggctacat
    19261 gcccactaca atccaaatca agttaagatt caatctattg ctttgcgcct tcgcggcagt
    19321 cttatctgcc tgttctcctg caatccaaac gcctgtgccc agccagaccc cgctgccacc
    19381 gactttgacg cctaccgaga ctaccacccc gacgcaaacc ccaacccctg caccgctaaa
    19441 gctggatatc ccggtccttt tagagcaatg tgagactctc agcattacaa acacagaagt
    19501 aattgtgagc ggtcatctct acctgccgga gttccggatt tatgggtaca gtgggtggaa
    19561 aggcatgaac ctgaaacaat tcctgcccaa cgaccagaaa ggcatcactg ccctgatccc
    19621 gcttggcgat ggacctaaca caatggacga actgccggtg tatttcacgc cgcgcgacct
    19681 gcgtgcgcgc tcggatagcg ggcagctgat tcttcacggg cacagtgtta gcatccaggg
    19741 tcgtgctgaa tatgtagtga gtgagggggt gccccgctgc caggtgtggg tggaaaagat
    19801 tgaatcgttg atgccagcgg agttgctcat tccacagact ttcgagattt ccaacttgct
    19861 taaagaacag aacatcggga gtaaatctca tccggaaatc gtttccacct gtgaattgat
    19921 gggaagaaag caacaaatgg tgtccataaa gggctcagtc atcgcggaga gcggtccgga
    19981 cctttgcaag caagaaatat gccgttttaa gctgacagat aagaccggca cggtgaccgc
    20041 gctttttctg ttggcggata cgcccaattc cataaacttc atctcaaacc aaggctggaa
    20101 gctgatggac caggatggaa ttccccgcga cctgctgaac ctggttttca ctggtacgct
    20161 gtttgttgat ccttctgatg gctgttcgtt attggtgtac cagatcaagg aacattgata
    20221 gtcaacctgt aatagaccgt ggctgataga tcgaccttcg cgcagcaccc tctggaaggc
    20281 tcggtcccga accaacaaaa aagaggctgt ctcattaatt ctgagacaac ct
//
LOCUS       DAOQAD010000026        20134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_2775_length_20134_cov_5.998655, whole genome shotgun sequence.
ACCESSION   DAOQAD010000026 DAOQAD010000000
VERSION     DAOQAD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 20134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20134
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2775_length_20134_cov_5.998655"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PKL60_03605"
     CDS             complement(<1..568)
                     /locus_tag="PKL60_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNW95254.1"
                     /translation="MLQKKNIAIIGPGVMGKTIALSLIQTGNLPPEQILMAGPNQDRL
                     NQLQAELGVRTTLSNAAAARAADVVILAVKPQRLDQAASALKGALEPGKLVISILAGA
                     PLSALEQKLGTRCLVRAMPNTPARIGMGITVWTKTAEVDEAQHEIAAQIMRTLGEEVF
                     VADEAYLDMATALSGTGPEARWPYPGRLH"
     gene            complement(997..2046)
                     /locus_tag="PKL60_03610"
     CDS             complement(997..2046)
                     /locus_tag="PKL60_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNW95255.1"
                     /translation="MDNFEAQSSPNKKKFWQYLIILVVLGLAVNLLLPQISDLTRSWE
                     VVKKMTWWAVGLAFVAVVFEYLSYGYCIHAIVHLHDKQLSTPKGTLIVMAATSVGLVA
                     GGWFAVAGSIFGSVRRETNDGTTATMAGILPTMLLNLSIEVVAILGILYLAIIGKLTN
                     SQLVQYIVFLILLTISTFGYLLALVLPKQTFRIINGVLWRWSRIRKKPYDPQKTQDMI
                     DNVILSWKELARGNWKKPFLGAFGYITFDMLAMFLIFQAVGYDIHPGVLLASYGIPYL
                     LAKIAFIFPGGIGVVEASMAAIQTSLGVPNEISVVAILGYRLFSFWLPTLLGFGAMGY
                     LSRAKKTFKQPAVQK"
     gene            2777..3394
                     /locus_tag="PKL60_03615"
     CDS             2777..3394
                     /locus_tag="PKL60_03615"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNW95256.1"
                     /translation="MTLSISSKTPVELPALPFAETALDPVISANTLSFHHGKHHKAYV
                     DKTLSLIAGTELEGKTLEEIVLAAYKEGKTGLFNNAAQIWNHNFYWKSLAPAGALDVP
                     AEAAALLKDSFESVEAFKKLFTEAAVGQFGSGWAWLIWDGSKLSIDKTGNALLPWVEG
                     KKPVLTVDVWEHAYYLDYQNRRQAYVEAVLEKLVNWEFMLANIKG"
     gene            complement(3594..3875)
                     /locus_tag="PKL60_03620"
     CDS             complement(3594..3875)
                     /locus_tag="PKL60_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95257.1"
                     /translation="MFSLPRARRKTRKKPLIPAAIDSSAADDTTHAAILEGKAELLRE
                     PPEFLRRQFTWIYARYVGKVGEAKSQEWIDDPENTLIKLVSEKVITWHW"
     gene            3994..5283
                     /locus_tag="PKL60_03625"
     CDS             3994..5283
                     /locus_tag="PKL60_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW95258.1"
                     /translation="MRKKRRRGNPFLIILLTAGIAFLVYFNVVVAPDINPPFVPTPTE
                     TRDPVSFEIEADALAVEGKFLSAIETYQAAINANPKNMDNYLKIARLQIYSGDYAQAQ
                     VNAENAILLNKSSADAFALLGWAKGLQQQYLEGERDAQTAIQLDPNNAIGHAIYAYLL
                     ALRVELGANELNTVDLAIEESRTALALDANLLEARWARGYVLEITSNYSEAVEQYEVA
                     VQLNANIAQLHLALGRNYMTLGKYDEAVFEFTKAYSLNPTDPLPNYFISRVYGSLGEW
                     AKAIQYGEQALKDGPTEAYLYANVGTMYYRSGSYNQAVLYLEKAVRGGTTESGAVIQG
                     LPLDYSTFVIETYSRYGLSLARINRCSEAVAVANGMLQTVPDDPDGVFNANEMIRICQ
                     QNLINPPTATPAPTATPVTGPSPTPQGTLAPTSTPTP"
     gene            5352..6602
                     /locus_tag="PKL60_03630"
     CDS             5352..6602
                     /locus_tag="PKL60_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95259.1"
                     /translation="MLLILVIVVLVAVLRSYAQGAIWPPFVPTPTPTRTVNSYMVEGN
                     THFLAGNLEKAISSFNQAVLMDPTNTALLVELAKIQVYSSNTLTTDADRRTRLAEALA
                     VIDQAKAIEPNNPDVLATRAFVLNWNANPTLAGENSQNLQNEAESEALMALQMDSKNT
                     LAMAYYAEILTDQYKWVQAREYMAQAMENGEQYMDVHRIQAYLYEVLGEYNLAIGEYQ
                     KAIEIYPNLTFLYNSVGRLYRHLEQYDVALEYFDRAASLNEQLGIKDPIPYMLIANTY
                     VRMGEAMVASRNAYKALTLSPYNADTYGQVGVIYYRARNYEGSIPLLQCAVRGCDAAT
                     SCEARECEDPEAEQIVIEGLPLSDTTVTYYYIYGSVLAGLHRPGDDKCEKALEVLAEV
                     RALYASNPTVMGIVEASEAICSGE"
     assembly_gap    7015..7114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7347..7640
                     /gene="groES"
                     /locus_tag="PKL60_03635"
     CDS             7347..7640
                     /gene="groES"
                     /locus_tag="PKL60_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNW95260.1"
                     /translation="MTIAMKPLGSRLVIEPKEQDEVTPGGIVLPDTAKEKPQKGTVIA
                     VGPGDRNEKGERIALDVKVGDLVLFAKYSGTEIKYEGKKLLILRESDVLAILA"
     gene            7666..9300
                     /gene="groL"
                     /locus_tag="PKL60_03640"
     CDS             7666..9300
                     /gene="groL"
                     /locus_tag="PKL60_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767614.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNW95261.1"
                     /translation="MAKQLVFSDEARRALKNGVDIVANAVATTLGPKGRNVALDRKYG
                     SPTITHDGVTVAKEIELKDPFENMGAQLLKEAATKTNDIAGDGTTTSTVLAHAMVSEG
                     LKNLAAGANPMLLKRGIETAAKAVAEAIHEQAINITTKEEIGSVATISAQDAEIGKLI
                     ADVMDKVGKDGVITVEESKGLEFETEYVEGMQFDRGYISPYFVTDNEHMEASLDDPYI
                     LIYDKKISAAADMVPLLEKMIQVGKREIVIIAEDVDGEALATLVVNKIRGMINVIAVK
                     APGFGDRRKAMLQDIAILTGATVISEETGRKLESATIEDLGRAEKVTTDKDNTTIVGG
                     KGNLSAIKGRIEQIRVEIENTTSDYDREKLQERLAKLSGGVAIIRVGAATETELKEKK
                     HRVEDALSATRAAVEEGIVAGGGVALVNAVAALSELKMDSTDAQIGVNIVRNALEIPM
                     RRIAENAGKDGSVVVENVRQKQAATGNKHFGYNVLSEEYSDLVTSGVIDPAKVTRGAL
                     ENAASIAAMILTTEALVTDIPEEKPAPAMPQGGMDY"
     gene            9550..10422
                     /locus_tag="PKL60_03645"
     CDS             9550..10422
                     /locus_tag="PKL60_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNW95262.1"
                     /translation="METEELPIKHILRFQQDEIDGHEIYKELAGLVRDPANSKIIAAL
                     SGDELGHYRTWRRYSGQEVKVNKLKVFAYKLLGRLFGYTFVIKMLEKDEDKASEGYEP
                     YKARFPELEAIIQDEDEHEEQLIAMIDEESLKYSGSVVLGLNDALVELTGTLAGLTLA
                     FQNTRLIALSGLITGIAAALSMAASEFLSTSSEETNKEPVKASIYTGLTYIVTVTLLI
                     LPYLLFSNYYAALAVMLIVGVLIIALFNFYSSVVREEPFLRNFGRMAAISLGVAALSF
                     GIGFLVRSVFGISV"
     gene            10478..11392
                     /locus_tag="PKL60_03650"
     CDS             10478..11392
                     /locus_tag="PKL60_03650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNW95263.1"
                     /translation="MLKAKTLGCLVLLGLALTACQPTAPMEPTPTAGFNQPALITASD
                     TPAPTPTPTSLPAAALVNGEVIPLSYYQNEVLRYRASFTEGETLPADAEIQATVLNSL
                     IDQLLLAQQARQGGFTFTDADVQGRIDALLAQLGSGSALTDWMQSNFYDDPEFRYALK
                     ISTEAAWQRDQIIAAVPTAVEQVRARQIFAQTASGAERALASLNSGVSFDELAWEYSP
                     ETGGELGWFPRGYLLFPDIETAAFLLAPGEHSDIIQTEIGYHIIQVMERSDAHPLSTD
                     ARLSLQTQALAQWLEAQRAGAQIVQVLP"
     gene            11461..12147
                     /locus_tag="PKL60_03655"
     CDS             11461..12147
                     /locus_tag="PKL60_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95264.1"
                     /translation="MARRKRGSCSSITIGIVLMIIILIVLLAASGIISAPVVHKTTPV
                     PSQPSTSSALPAGQASVTLAQTLASTPTVTAQPSLTPTTTGTPTPSRTPSPTPTATEK
                     SMPFVVRGTPEGLSHTLFHPEYACEEYLFIGGQVWDLQDSPLMGLVVRLGGTYGGDIV
                     DMTVESGSSAVYGRSGYEFAIANKQIAESELYIQLEDASGELLSARTILNITGSCQAN
                     LIIVNFKQVR"
     gene            complement(12179..13393)
                     /locus_tag="PKL60_03660"
     CDS             complement(12179..13393)
                     /locus_tag="PKL60_03660"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HNW95265.1"
                     /translation="MKIRSITVFCAPGYPANRLLLQHLGIFANHARALFTQAGFEVES
                     LRLALPPFSSFVEPRRLAEAARELSIEAHAEGFEYLSLGPAQTGDLEAYPRIPEALAG
                     THGVFFSGLLTTPQGAVSLPAAKACAQIITQLAALSPDGFSNLNFCASANVPPYAPFF
                     PAAYAENAAPAFALAMEAADLALNVFSAARSLEEARQTLISEVESAANKLESAAQKLS
                     QIYHYVFKGLDFTLAPFPQENISIARALESLGLPAFGLSGSVFASAFLTDTLDRARFT
                     RTGFNGLMLPVLEDSGLAQRAAEGSLSAEELLLCSAVCGTGLDVIPLPGDTNAEQLYP
                     LLLDLAALALRLNKPLTARLMPIPGKKAGDPTEFDFTYFANSRVMPLRAEKSVGLLSG
                     SESVPIARREPR"
     gene            complement(13390..14181)
                     /locus_tag="PKL60_03665"
     CDS             complement(13390..14181)
                     /locus_tag="PKL60_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNW95266.1"
                     /translation="MTQDIISSPQNHYAQEARALLNQPKARRKLQAFAAEGARLVEDG
                     LGSGARARYLLVRAAHSARVDAVLGVSSAAIPTVLVEDKLFDSLAGTITSQGIIGVFD
                     LPAWQLPDALDFVLILDQMRDPGNLGTILRSAEAAGAQAVFLSPGTADAFAPKVLRAG
                     MGAHFRLPILTLEWETLAVVLAGLNVFQADMDGEISCWQADFRSPCALLIGGEAEGIS
                     PEAAELVTRSVRIPMAGRSESLNAAVSAGILLFEVLRQRSIGETN"
     gene            complement(14205..14846)
                     /locus_tag="PKL60_03670"
     CDS             complement(14205..14846)
                     /locus_tag="PKL60_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNW95267.1"
                     /translation="MRIPQRLSDIIPRMPAFHAPDPNAAPTDHRYIVLSDTHVGDRVN
                     HLDPRLLENIRAFQPEGILHAGDLSSPEVLEELQAIAPVLAVQGNRDWMRRIKLPREQ
                     RFKVNGVKVVLTHGHVSMWHYVLNYLRLLITGRKADVRTFRERLEKLYPEADLILFGH
                     THYQLHEVVGGKHFVNPGAAYPHLLNQRNIQYCELTISAAGQIQVRKLSLPDW"
     gene            complement(15152..15373)
                     /locus_tag="PKL60_03675"
     CDS             complement(15152..15373)
                     /locus_tag="PKL60_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95268.1"
                     /translation="MKELAVRFLYYSFAQSLFKGRLHAEGDAGKRCRRLSFSGKQLAS
                     AARRMWVFWSRLGLLQAELPDFYALYAHQ"
     gene            complement(15419..16435)
                     /locus_tag="PKL60_03680"
     CDS             complement(15419..16435)
                     /locus_tag="PKL60_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW95269.1"
                     /translation="MNANFNPMIKPDLPGVVYRGYRGEEDFPVMVQIWNQMRAADLTE
                     GSFTLEDAEINFRHLYNCDPFRDMIFAEMDGVPAAYGRVMWLQEGPRKAFYQIFNMNP
                     ALQGSGIDIPMQTWMEQRQLEIAADLADEGEKVFIQCSASEDTHRMGLLESFGYKAVR
                     WYFSMSRDLNELPDVDLPEGIEVRPAFPSEYRKVYDRSNEAFSEHWGFVEPREEDYQT
                     WKDGRWFQPMLWQVAWDGDEPVGQVENYIDALENEKLGRLRGYTEGISVLKAYRGKGI
                     ARALIARSLRMLKAMNLEEAALSVDSDNACGALRLYENMGYRTYQTMVEWRKPMPELQ
                     LESR"
     gene            complement(17168..17752)
                     /locus_tag="PKL60_03685"
     CDS             complement(17168..17752)
                     /locus_tag="PKL60_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNW95270.1"
                     /translation="MKKIIVLCLCLLLALAVVPPMAAKALSPGRGSQAPAALNPQQEA
                     TSSYVNTIITSTPNADGSIIHIVREGETLWAIAMAYGLTGAEIMTNSGNSPLATDVYA
                     GQILIIRLPNPATATLTLTPTPTRITPQPTQPRPSLTPVPSHTPQPIPTETPVPPVFQ
                     RAFADGKTVGLVLAGLSAVGVLVVLVFGFLKKAK"
     gene            17906..19177
                     /gene="serS"
                     /locus_tag="PKL60_03690"
     CDS             17906..19177
                     /gene="serS"
                     /locus_tag="PKL60_03690"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNW95271.1"
                     /translation="MLDLKLIREQPELIRESLRKRHMDSGIVDQIIGQDELRRALILE
                     VEAKKAERNAASKEIGRMKDAAARQAKIDAMRELGDRISALDEKLKSVEAELHLSVAG
                     LPNIPDPDVPVGLDDKDNIVVRTIGEKPVYDFEPLAHWDLGPRLGMINFEAGVRLAGT
                     RFYVLEGAGARLERALIFWMLDLHIRQGYREIYPPWMVRQEVMFASGQLPKFADNLYH
                     DAEEDFWMVPTAEVPLTGMMMGEVLDEATLPLNLTAYTACFRREKMSAGRDVRGIKRG
                     HQFDKVEMYKYCQPKDSPAQLDQMVADAEETCRQLGLTYRVLLQSTGDLSFGSHKTYD
                     LEVWAPGCQEWLEVSSVSNIGEFQARRANIKYRPADGSSPRYLHTLNGSGLGLPRTLI
                     AVMETYQQADGSIRVPEPLLPFMGGMEVIRA"
     gene            19174..19707
                     /locus_tag="PKL60_03695"
     CDS             19174..19707
                     /locus_tag="PKL60_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNW95272.1"
                     /translation="MSTSPAWVGTLILILTALFQLIGLVGLVLVFFPGPTVAWVGQLI
                     WAIFTRFNYGHEPWQFTLTIVFFVISTLLMLVGSFLDNLMMAGRARKQGSPWWEIGLS
                     WFAMIVGGILLTPLGGLAAALLTLFLVEYQRLGKKQKEAWAATKAVALGWGWSTLIRV
                     GIVLVMIALWLVSVVWF"
     assembly_gap    19970..20069
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4391 a         5441 c         5913 g         4189 t
ORIGIN      
        1 gatgaagcct acctggatat ggccaccgcg cttccgggcc ggtgcccgag agcgcggtgg
       61 ccatatccag gtaggcttca tccgccacga agacttcctc gcccagggtg cgcataatct
      121 gcgcggcaat ttcgtgctgg gcttcgtcca cctccgcggt ttttgtccat acggtaatgc
      181 ccatgccgat gcgggcgggg gtgttgggca tggcgcgcac caggcagcgc gtccccagct
      241 tctgctccag tgccgaaagc ggcgcgccag ccaggatgga gatgaccagt ttcccgggct
      301 ctaacgcacc tttcagggcg ctggcggcct ggtccagacg ctgcggtttg accgccagga
      361 tgaccacgtc agctgcgcgg gcagctgcgg cgttgctgag cgtcgtgcgt acgccgagct
      421 cggcctgcag ttggttcagt cggtcctggt tcgggccggc catgagaatt tgctcaggcg
      481 gcagattacc tgtctggatc aggctcaaag cgatggtttt gcccatcacg ccgggaccga
      541 tgatggcgat gttcttcttt tgcagcatat ttcctccaaa aaagccgaaa gtttccgctg
      601 tctgcctgta gggcctgctc cgcgaaaaac ttgtccgatg acttttgtca atctaaaacc
      661 ctgatgagcc cagagcccca gccaagaaag cttctttttc acaacaaggg tcttccctgt
      721 gttcaggttg actggaattt cattgacttg aagcattctt cctgacgatt accctgcccg
      781 ccaaaatgaa atgattgctc tcctggacgc tggaaaaacg attcctattt tgaaaaaaag
      841 attcctcgct gcggtcagaa gggcagggca agaatccgat gaaaagctct tgcagcccga
      901 catccctgca tgctttgtaa aggcgcacca tcgcactcca tccaaaccgc tcaaggggcg
      961 tgcgctgatg ccggcgttga cgaccgtcgg cgcggactat ttctgcacgg cgggctgttt
     1021 gaaagttttt ttagcacggc tgaggtagcc catggctccg aaaccgagca aggtgggaag
     1081 ccagaaggag aacaaacgat agccgaggat agctaccacg ctgatttcgt tcggaacgcc
     1141 cagactggtc tggattgcag ccatggatgc ctcaacgacg ccaataccgc cggggaagat
     1201 gaacgcaatc ttggccaaca ggtacgggat gccgtagctg gcgagcagca cgccggggtg
     1261 gatgtcgtag cccactgcct gaaaaatcag gaacattgcc agcatgtcga atgtgatgta
     1321 accaaaagcg cccaaaaaag gttttttcca gttcccgcgc gccaattcct tccacgaaag
     1381 gataacgtta tcaatcatgt cctgcgtctt ctgcgggtca taaggttttt tgcggatacg
     1441 gctccaacgc cagagaacgc cattgatgat gcggaaagtt tgtttgggca ggaccaacgc
     1501 aagcaggtag ccaaaggtag aaattgtgag caggatcagg aatacgatgt actggacgag
     1561 ctgcgagttc gtaagtttgc cgattatcgc cagatagagg atacccagga tcgcgacaac
     1621 ctcaatcgaa agattcaaca gcatggtggg cagaatccct gccatcgttg cggtcgtgcc
     1681 gtcgttagtt tcgcgccgta ccgagccaaa tatggagccc gctaccgcaa accatccccc
     1741 ggcaaccagc ccgacgctgg tggcagccat gacaatcagt gtgcctttgg gggtggaaag
     1801 ctgcttgtca tgcaaatgga cgatggcatg aatgcagtag ccgtagctga gatattcgaa
     1861 taccaccgcc acaaatgcca ggcccaccgc ccaccaggtc atctttttga cgacttccca
     1921 ggagcgggtc aggtccgaaa tctgcgggag cagcaggttg actgccagcc cgagcacgac
     1981 caaaataatc agatactgcc agaacttttt tttgttcggc gaagattggg cttcaaaatt
     2041 atccatacga ttgcgcggga tcaagccggt cttgatcacg cgttcctcaa gtatacatga
     2101 ttccggtgcg gcgcaaattg gatagcaaac gagcgccgac gaagcgtaat ccgtgagtgt
     2161 atgcgcgggg tatctgtcat gtgatggtct tctctcagtt ttctttgctc ccaggcaagg
     2221 cgagccgcag gtctgatgga aaaaaagagc aatattcgtg ccaacgcgct tctgcaggcc
     2281 caaaaagtga aatgcggcag cttttgccct tcggaatgtc gaggcgaaac agaaacatcc
     2341 aaaggagtgg atgaagccat caggacggat tttcctcgag gctaaccggc ttatttcttc
     2401 tttacgatcc tgtctcacgc ttgccctaag cctgaccctt cccgggcgat cgttcatcag
     2461 gattttaggt taattttcgt ttggggtgga gtaggctgca aatccctcgg atatcagggt
     2521 atgagcattc ctttggaaaa atcctggagg tatcgaatga tggtttccta gccgggaaat
     2581 ttctattttg ctatttcggg gcaggattcc tttgctcttc gcttttgccc tccggaaaag
     2641 taactccgga aaagtaacaa ttcccactta caggtataat atacaagcgc gggggcattt
     2701 atctgaccac tgcgcgggca tccgatttac agcccgtaag attgggcttt ttaccaaaaa
     2761 aagaaaagag gaaaacatga cactttcaat ttccagcaag acccctgtag aacttcctgc
     2821 tcttcctttt gccgaaactg ccctcgatcc ggttatctcc gcgaacacgc tcagcttcca
     2881 ccacggaaaa caccacaagg cttatgtgga taagaccctc agtctgattg ccggcactga
     2941 actggaaggc aagaccctgg aagaaatcgt attggcagca tacaaggaag gcaaaaccgg
     3001 gctttttaac aatgccgctc agatctggaa ccataatttt tactggaaaa gcctggcccc
     3061 ggccggcgcc ctggacgtgc ccgccgaagc cgcggcgctg ctgaaagaca gcttcgagag
     3121 cgtggaagcc ttcaagaagc tgttcacgga agcagccgtc ggtcagtttg gcagcggttg
     3181 ggcctggttg atttgggacg gcagcaagct gagtatcgat aaaaccggca acgcgctgct
     3241 gccctgggtg gaaggcaaga aacccgttct gaccgtggac gtttgggagc acgcctatta
     3301 cctggattac cagaaccgcc gccaggccta tgtggaagcc gtgctggaga aactggtcaa
     3361 ttgggaattc atgctcgcga atatcaaagg ttgagttcgt tttctgtccg cggcttgccg
     3421 cggtgagatc gaaatatgat ggcgcggaga aattccgcgc ctttttcttt gcctggcgcg
     3481 caccgaagtt gcgaaatggg gggtgcgctg cccttacgtg gggtggatga gccgcccgag
     3541 cgatttatgg ccagggagtt tggatgcctg agtttttttg ctgagtaggc cttttaccag
     3601 tgccaggtga taactttctc ggagaccagc ttgatcagcg tattttccgg gtcgtcgatc
     3661 cattcctggg atttcgcctc gcccactttc cctacatagc gggcataaat ccaggtgaac
     3721 tggcggcgca gaaactccgg gggttcacgc agcagctcgg ctttgccctc cagaatcgcg
     3781 gcgtgggtgg tgtcgtccgc tgcgctgctg tcaatcgctg cggggatcag cggttttttc
     3841 cgggtctttc gccttgcgcg tggaagggaa aacactgccg tcccaggtat accggagcag
     3901 gaggcagtgc ggctgcccgt atcaacttgc ccgacggcct ttaagaattc atgttattat
     3961 gcacccatgt atttaagagg aagtaatctt tccttgcgaa agaagcgccg gcgcggcaac
     4021 ccattcctta tcatcctgtt aacggctggg attgcctttt tagtctactt taacgtggtt
     4081 gtcgcgcccg atatcaaccc gccttttgtg ccgaccccga ccgaaacgcg cgacccggtt
     4141 tcctttgaaa ttgaagctga cgcgctggct gtggaaggaa aattcctgtc cgcgattgag
     4201 acctatcagg ctgccattaa tgccaacccg aagaatatgg ataattacct gaaaatagcc
     4261 cgtctccaga tttactccgg cgattatgcc caggcgcagg tcaacgcgga aaacgccatc
     4321 ctgctgaaca agagcagcgc cgacgcgttc gcgctgttgg gttgggcaaa agggctgcag
     4381 cagcagtacc tggaaggcga acgcgatgcc cagacggcta ttcagctgga cccgaacaac
     4441 gccatcggtc acgccatcta cgcctacttg ctggcgctgc gggtggaact cggcgcgaac
     4501 gaactgaata ccgtcgacct ggccatcgag gaatcccgca ccgcgctggc attggacgca
     4561 aacctgctgg aagcccgctg ggcgcgcggc tatgtgctgg aaatcaccag caactattcg
     4621 gaagctgtgg agcagtatga ggttgccgtt caattgaatg ccaacatcgc ccaactgcac
     4681 cttgccctgg ggcgcaatta catgaccctg ggcaagtatg acgaagccgt tttcgagttt
     4741 accaaagctt attccctcaa tccgaccgac cctttgccga actacttcat ttcgcgggtg
     4801 tacggaagcc tgggtgaatg ggccaaagca attcagtacg gcgaacaagc gcttaaggac
     4861 ggccccacgg aggcctacct gtacgcgaac gtcggtacga tgtattaccg cagcggctct
     4921 tataatcagg cggtactcta cctggaaaaa gcagtccgag gcggcaccac cgagagcggc
     4981 gcggtgattc aggggctgcc gctggattat tccacctttg tcatcgaaac ttacagccgc
     5041 tacgggctct cgctggcgcg gattaaccgc tgcagcgaag cagtcgccgt tgcgaacggg
     5101 atgctccaga ccgttccaga cgacccggac ggtgtgttta acgccaacga gatgattcgg
     5161 atctgtcagc agaacctgat taacccgcct actgccacac ctgccccaac ggcaacgccg
     5221 gtaaccggtc cttccccgac tccgcaggga acgctggcgc cgaccagcac gcccacgcct
     5281 tagctatgta catcaaagaa cggcaaaacc tgtttcgaaa tccaaagaat cagtctaatc
     5341 cgtaccggat tatgctgctg attttagtga ttgtggttct ggtggcggtg ctgcgcagtt
     5401 atgcgcaagg cgccatctgg ccgccattcg tccccacgcc cacgcccaca cgcaccgtga
     5461 attcttacat ggtggaaggc aatactcatt tcctggcagg caacctggag aaggcgatat
     5521 cctccttcaa tcaggcggtg ctgatggacc cgaccaatac agccctgttg gtggaattgg
     5581 ccaaaatcca ggtgtactca tccaacacac taaccaccga cgccgaccgg cgcaccaggc
     5641 tggcggaagc gctggcggtg atcgaccagg ccaaggcaat tgagccgaat aacccggatg
     5701 tgcttgccac gcgcgccttt gtgctcaatt ggaatgccaa tccgacattg gcaggcgaga
     5761 attcccagaa cctgcagaac gaagcggagt ccgaggcgtt gatggcgctg cagatggaca
     5821 gcaaaaacac gctggcaatg gcgtactacg cggaaatcct gacagaccag tataagtggg
     5881 tgcaggcgcg ggagtacatg gcgcaggcaa tggaaaatgg cgagcagtat atggacgtgc
     5941 accgcattca ggcgtacctg tacgaggtgc tcggagaata taacctcgcg attggggagt
     6001 accagaaggc catcgaaatc tatcccaacc tgacctttct gtacaattcc gtgggcagac
     6061 tgtaccgcca cctcgagcag tacgacgttg ccctggaata ttttgaccgg gctgccagcc
     6121 tgaacgagca gctgggcatc aaggacccca ttccgtatat gctgattgcc aacacttatg
     6181 tacgtatggg tgaggcgatg gttgcttcgc gcaatgcgta caaggcgctg accttgagcc
     6241 cgtacaatgc tgatacctat gggcaggtgg gggtcatcta ttaccgcgcg cgcaactacg
     6301 aaggttccat tccgctgctg caatgcgcgg tgcgaggctg cgacgccgcg accagctgtg
     6361 aggcgcgcga atgcgaggac ccggaggctg agcagattgt gattgaggga ctgccgcttt
     6421 cggatacgac cgtcacatat tattatatct acggctctgt gctggccggt ttgcaccgcc
     6481 cgggtgatga caaatgcgag aaggcgctcg aagttctggc ggaagtgcgc gcgctgtacg
     6541 caagtaaccc gaccgtgatg ggaattgtgg aagccagcga agcgatttgc agtggggagt
     6601 agagaaagtc tgcgcgtcct gtgggtattg acgggctgcg cttgttgtgt caatgttttg
     6661 gtcattctga acgaagggta gaattgttct gttgaaatgc gagctcctgc gctgcgctca
     6721 ggatgacaag ccgcataagc tggatgcagg ggcgcgggag cggaactcaa ttgcggctcc
     6781 acaaacccgg cggccaaagt ttgtcgggtt gcgctgccgc tccacccgac ctacgtcctg
     6841 cgcttttgac aaagtcatag cattcagaat gacaataaag acctgtcgtc cagagcttac
     6901 gaaggatctg ttcgtttcgg cacagagctc ctgcgctgcg ctcaggatga caagccgcat
     6961 aagctggatg caggggcgcg ggagcggaac tcaattgcgg ctccacaaac ccggnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgctc aggatgacag aaaaacgctc
     7141 aggatgacag gaataaacgt tcaggatggc gggaattaac gctcccgata gcgaacagaa
     7201 cccggtaaac cccaatatta aaagttcgta agaaatctat atccgctctt gacttctcgt
     7261 tttgggggtt gtatgattag gcatgttagc actcaattaa taggagtgct aaaatattga
     7321 aatcaaacca taacttggag gaatatatga caatagcaat gaaacctttg ggaagccgtc
     7381 tggtgattga accaaaagag caggatgaag tcacccccgg tggtattgtt ctgcccgata
     7441 ccgcaaaaga aaaaccccag aagggcaccg ttatcgcggt tggtcctggc gaccggaacg
     7501 aaaaaggcga acggattgcg ctggacgtga aggttggcga tttggtgctt ttcgccaagt
     7561 actccggcac tgaaatcaag tatgaaggca agaaactgtt aattctgcgc gagagcgacg
     7621 ttctcgctat ccttgcttaa tttttgcgat tgaaggagaa aatctatggc taaacaactt
     7681 gttttttcgg atgaagcacg ccgcgccctg aaaaacggcg tggatattgt ggcgaacgct
     7741 gttgccacca ccctcggccc caaaggacgc aacgtcgctt tggaccgcaa gtacggttcc
     7801 cccaccatca cccacgacgg cgtgacggtt gccaaggaaa tcgaactgaa agaccccttt
     7861 gagaatatgg gcgcgcagct gcttaaagaa gccgcgacca agaccaacga tatcgccggc
     7921 gacggcacca ccacctccac ggtcctcgcg cacgcgatgg tcagcgaagg cctgaagaac
     7981 ctggctgccg gcgccaaccc gatgctgctg aagcgcggta tcgagaccgc cgccaaagcg
     8041 gttgccgaag ccattcacga gcaggcgatt aacatcacca ccaaggaaga aatcggttcc
     8101 gttgccacta tttccgccca ggatgccgaa atcggcaagc tcatcgcgga tgtaatggac
     8161 aaagttggta aagacggcgt catcaccgtg gaagaatcca agggtttgga attcgaaacc
     8221 gagtacgtgg aagggatgca gttcgaccgc ggttacatct ccccctactt cgtaaccgac
     8281 aacgaacaca tggaagccag cctggacgac ccgtacattc tgatttacga caagaagatt
     8341 tccgccgcgg cggacatggt gcccctgctg gagaaaatga tccaggtcgg caagcgcgaa
     8401 atcgtgatta tcgctgaaga tgtggacggc gaagcgttgg caaccctggt tgtgaacaaa
     8461 atccgcggga tgattaatgt catcgccgtg aaagcccccg gatttggcga ccgccgcaag
     8521 gcgatgctgc aggatatcgc gatcctgacc ggcgccaccg taatctccga ggaaaccggc
     8581 cgcaagctgg aaagcgcgac catcgaggac ctcggccgtg ccgagaaggt tacgactgac
     8641 aaagacaaca ccaccattgt gggcggcaaa ggcaatttgt ccgcgatcaa aggccgcatc
     8701 gagcaaatcc gtgtggaaat tgaaaacacc accagcgatt acgaccgcga aaagctgcag
     8761 gaacgcctgg ccaagctttc cggcggcgtt gccatcatcc gtgttggcgc agcgaccgag
     8821 accgagctga aggaaaagaa acaccgcgtg gaagacgctc tctccgcgac acgtgccgca
     8881 gtggaagaag gcattgtggc tggcggcggc gttgccctcg tgaatgctgt cgcggctttg
     8941 tccgaactca agatggacag caccgatgcc cagattggcg tgaatatcgt gcgcaacgcg
     9001 ctcgaaatcc cgatgcgccg catcgctgaa aacgccggca aagacggttc cgtggttgtc
     9061 gagaatgtgc gccagaagca ggctgctacc ggcaacaagc acttcggcta caacgtcctc
     9121 agtgaggaat acagtgacct ggtcaccagc ggcgtgattg atcccgccaa ggttacccgc
     9181 ggcgctctgg aaaacgctgc ttccatcgcg gcgatgatcc tgactaccga agccctggtc
     9241 accgatatcc ccgaagagaa accggcgccc gcaatgcccc agggcggcat ggattactaa
     9301 aaaccgcttt aggattgaga agaaaccgtc cgcgagggcg gtttctttgt atattaaagg
     9361 aaattcctgt ggaacgcggg ggcaagcgcg gctcagcggt acgcacaggc gaggggtgag
     9421 aaaatggggg cgatgatttt gactggcgtt cagccgctgc ctggacgcgc gcggtccgta
     9481 gactgctaca ctgcacagaa tcaggttgcg gatttgatac aatgaggcgg aaggaaggaa
     9541 caggaaacta tggaaacaga agaattgcca atcaaacata tcttaagatt ccagcaggac
     9601 gaaatcgatg gacatgagat ttataaggag ctggccgggc tcgtccgcga ccctgccaac
     9661 agcaaaatca tcgcggctct ctcgggggac gagctggggc attaccggac ctggcggcgc
     9721 tacagcggcc aggaagtaaa ggtgaacaag ctcaaagttt tcgcgtacaa actgcttggg
     9781 cgactgtttg gctacacctt tgtcatcaag atgctcgaga aggatgaaga caaggcttcc
     9841 gaaggatacg aaccctacaa agcgcgcttt ccggagctgg aagcgatcat ccaagatgaa
     9901 gatgagcatg aagagcagtt aatcgccatg attgacgaag aaagcctcaa gtattcgggg
     9961 tcggttgtgc ttgggctgaa cgacgcattg gtggaactga ctggcaccct ggcgggtctc
    10021 acgctggcct tccagaatac gcgcctgatc gcgctgagcg gtttgattac aggcatcgca
    10081 gccgcgttat ccatggcggc ttcggaattc ctctccacaa gctctgaaga aaccaataag
    10141 gaaccggtga aagcctcaat ttacaccggc ctgacttata tcgtcacggt gacattactg
    10201 attctgccgt acttactctt cagtaattac tacgctgccc tggcagtgat gttgattgtg
    10261 ggcgtgctga ttatcgctct gttcaacttt tacagttccg tagtgcgcga agagcctttc
    10321 ctcaggaatt ttggcaggat ggctgccatc agcctggggg ttgccgccct cagctttggg
    10381 ataggttttt tggttcgcag tgtgttcggc atttcggtgt aaaccgtttt ccgggcgacc
    10441 ttgagacttc agtggcattc gtgagtataa tggagctatg ttaaaagcga aaacattggg
    10501 gtgcctggtt ctgctggggc tcgcgctgac tgcctgccag ccgaccgccc ccatggaacc
    10561 caccccaacg gccgggttta accaacctgc gctgattacc gcttcggaca cacctgcccc
    10621 aacgcccacc cccaccagct taccggcggc ggcgctggtg aacggcgaag tcatcccgct
    10681 gagctactac caaaacgagg tactgcgcta ccgcgcctcc ttcacggaag gcgaaacgct
    10741 gcccgcggac gcggaaattc aggcgaccgt cctcaattca ttgattgacc agcttttgct
    10801 tgcccagcag gcgcgccagg gcggcttcac gttcacagac gcggacgtgc aggggcgcat
    10861 tgacgcattg ctcgcccagc ttggctcggg cagcgcgctg acggactgga tgcagagtaa
    10921 tttctatgac gacccggagt ttcgttacgc cctaaaaatc agcacggaag ccgcctggca
    10981 gcgcgaccag attattgccg cggtgccgac cgcagtggaa caggtgcgcg cccgccagat
    11041 ttttgcccag accgcgtccg gggcggagcg tgcgctcgcc agcctcaact ccggtgtgag
    11101 ttttgacgag ttggcgtggg agtacagccc tgaaaccggc ggcgaacttg gatggtttcc
    11161 gcgcggatat ctgcttttcc ctgatattga aactgcggcg tttttgctcg ctccggggga
    11221 acattccgat atcattcaga ccgaaattgg ctaccacatc attcaggtga tggagcggtc
    11281 tgacgcgcac ccgctcagca ccgacgcccg cctaagcctg cagacgcaag cgctggcgca
    11341 gtggttggaa gcgcagcgag ccggagcgca gattgtccag gtactgccgt gagaaagtcg
    11401 gggccggtgc cgatggttaa gcggcgcgtg tccgcggaat cccagagcgg attgcccagc
    11461 atggcccgca gaaagcgggg ttcctgctcc agtattacca tcggcattgt gctgatgatt
    11521 attatcctga ttgttttgct ggcggcttcc ggcatcattt ccgcgccggt cgtgcacaaa
    11581 accacccctg tgcccagcca accctccacc agttccgcac tgccggcagg gcaggccagc
    11641 gttacgctgg ctcagacgct cgcgagcacc cccactgtga ccgcgcagcc ctcgctcacc
    11701 cctaccacca cgggcacccc gacgccgagc cgtaccccca gccccacgcc gactgctacc
    11761 gaaaagagca tgccttttgt agtgcgcggc acgccggaag gcctttcgca tacgctcttc
    11821 cacccggaat acgcctgcga ggaatatttg tttattggcg ggcaggtctg ggatttgcag
    11881 gattcgccgc tgatgggttt ggtggtgcgc ctgggcggca cctacggcgg ggacattgtg
    11941 gatatgacgg tggaaagcgg ttcctccgcg gtttacggcc gctccggtta cgaattcgcc
    12001 atcgcgaaca agcaaatcgc cgagagcgaa ctctatatcc aactggaaga cgcgagcggt
    12061 gagctgctat cagcgcgaac gattctcaat atcaccggct catgccaggc taacctgatt
    12121 atcgtcaact ttaaacaggt tcgttaatcc cccacgaaga aaacaccaac cctgggaact
    12181 atcgcggttc ccgccgggca atcgggacgc tttccgaacc cgagagcaga ccaacgctct
    12241 tttccgcgcg cagcggcatc acgcggctgt tggcgaagta agtgaagtcg aattcggtcg
    12301 ggtcgccggc ttttttaccg gggatgggca tcaggcgggc agtaaggggc ttgttcaggc
    12361 gcagcgccag ggcagccagg tccagcagca gggggtagag ctgttcggcg ttggtgtccc
    12421 ctggcagcgg gatcacgtcc agaccggtgc cgcatactgc cgagcaaagc agcagctcct
    12481 ccgcgctcag gctgccttca gccgcccgct gcgccaggcc ggaatcctcc aggacgggca
    12541 gcatcaggcc gttgaagccc gtgcgggtga agcgcgcgcg gtccagcgta tccgtcagga
    12601 aggctgacgc gaacaccgag ccggagagtc cgaaggctgg cagccccagg ctttccaagg
    12661 cgcgggcgat ggaaatattt tcttgcggga agggggcgag cgtaaaatcc agccccttga
    12721 acacgtagtg gtatatctgg ctgagctttt gcgcggcgct ttccagcttg ttggcggcgc
    12781 tttccacttc cgagatcaac gtctggcggg cttcttccag gctcctggct gctgaaaaca
    12841 cgttcaaagc caggtcggcc gcttccatgg ccagcgcgaa ggcgggcgca gcgttctctg
    12901 cgtaggctgc cggaaagaag ggcgcgtagg ggggcacgtt cgcagacgcg cagaagttca
    12961 gattgctaaa gccgtccggg ctgagagcgg caagctgtgt gatgatctgc gcgcaggctt
    13021 tggctgccgg aagtgacact gcgccttgcg gcgtggtgag gaggcctgag aagaatactc
    13081 cgtgcgtgcc tgccaatgcc tccggaatgc gcgggtaagc ttccaaatca ccagtttggg
    13141 cgggaccaag cgagaggtat tcaaagcctt ccgcgtgcgc ttcgatggag agctcccggg
    13201 cggcctcggc gaggcgccgg ggttcaacga aactggaaaa aggcggcagc gccaggcgca
    13261 ggctctcgac ctcaaagcct gcctgagtga aaagggcgcg ggcgtgattc gcgaagatgc
    13321 ccagatgctg caggagcagg cggttagccg ggtatccagg cgcgcaaaat acggtgatgg
    13381 aacgaatttt caatttgttt ctcctatgct gcgctgacgg agcacttcaa agagcaaaat
    13441 gccggctgag acggcagcgt tcaggctctc gctgcggccc gccatcggga tgcgcacgct
    13501 ccgggtgacc aattcagccg cctccgggct gatgccttcc gcctcgccgc caatcagcag
    13561 ggcgcaagga ctgcgaaaat cagcctgcca gcagctgatt tcaccgtcca tatcagcctg
    13621 aaaaacgttc aacccggcaa ggactacggc gagtgtttcc cactccagcg tgagaattgg
    13681 caggcggaaa tgcgcgccca tcccagcgcg caggaccttg ggggcgaaag cgtctgcggt
    13741 gccggggctg aggaacacgg cttgcgcgcc ggcggcttcc gctgaacgca ggatcgtacc
    13801 caggttgccg gggtcgcgca tctggtcgag gatgagaacg aagtccagcg cgtccggcag
    13861 ctgccaggct ggcaggtcaa acacgccgat gatgccctgg ctggtgatgg taccggcgag
    13921 ggaatcgaag agcttgtctt cgacaaggac agtgggaatc gccgcggagg atacacccag
    13981 cactgcatcc acgcgcgcgg agtgcgcggc gcgaaccagc agatagcgcg cgcgggcgcc
    14041 tgagcccagc ccgtcctcca ccaggcgcgc gccttccgcg gcgaaggcct gcagtttgcg
    14101 gcgggctttg ggctggttaa gcagcgcgcg ggcttcttgc gcgtagtggt tctgggggga
    14161 gctgatgata tcctgtgtca tggcggctga aatgattata catgctacca atcgggcagg
    14221 ctaagcttgc gcacctggat ctgcccggcg gcgctaatcg tcaactcgca gtactggata
    14281 ttccgctggt tcagcaggtg gggataggcc gcgcccggat tgacaaagtg cttgcccccg
    14341 acgacttcgt gcagctgata atgggtatgc ccgaaaagga ttaaatctgc ttccgggtag
    14401 agcttttcca ggcgctcgcg gaaagtgcgc acgtccgctt tgcgcccggt gatgagcagg
    14461 cgcaggtagt tcaacacgta gtgccacatg ctgacatgcc catgcgtgag tacgactttg
    14521 actccgttga ccttgaagcg ctgttccctg gggagcttga tacggcgcat ccagtcgcgg
    14581 ttgccctgca ccgccagaac aggcgcaatt gcctgcagct cttccaggac ttccggagag
    14641 ctgagatccc cggcgtgcag gatcccttcc ggttgaaagg cgcggatatt ttccagcagg
    14701 cgcgggtcca ggtgattgac ccggtcgcca acgtgtgtat cggagaggac gatgtagcgg
    14761 tgatctgtgg gggcggcgtt ggggtcgggg gcgtgaaaag cgggcatgcg cggaattata
    14821 tcagagaggc gctgggggat gcgcatatgc gggcagattt gtggctttca gactggcggt
    14881 cgttatgctg gcagaacaga cgttagtccc ctaaacttct cctgggagaa ggtgcccgta
    14941 gggtggctga gggtgttttc gggtgtaagt ttggtacagt cgtagggcgg tttgcgctct
    15001 tcaatccacc aatgctgtca ggtttaaggg ttattcagcg cagtaaggac gcatccgaag
    15061 cgataatttc aggcgcagcg gcgaagcatc cccaggaaag gaactgacct gcctggtttg
    15121 tcgctaaagg atgcttcgcc gctgccattg cttattgatg tgcatacagc gcataaaaat
    15181 cgggtaattc tgcttgcagt aaaccgagcc gcgaccagaa aacccacatc cgtctcgctg
    15241 cgcttgccag ctgcttcccg gagaagctta atctgcggca gcgcttgcca gcatcgcctt
    15301 cagcgtgcag gcggcctttg aagaggcttt gcgcaaacga ataatagaga aacctgacgg
    15361 ccaattcttt catgtgttcc gcaggcgctg caagaactgt gttcccgccc gcggaagctt
    15421 atctgctttc cagctgcagc tccggcatgg gcttacgcca ttccaccatc gtttggtagg
    15481 tgcggtaacc catgttttca tacaagcgta aagctccgca ggcgttgtcg gaatccacgg
    15541 aaagggcggc ttcctccagg ttcatcgcct tgagcatgcg cagcgagcgc gctattaacg
    15601 cgcgggcgat acctttcccg cggtaggctt tcagcacgct gataccttcg gtgtagccgc
    15661 gcagcctgcc cagcttttca ttctccaacg cgtcgatata gttttccacc tgccctacgg
    15721 gttcatcacc atcccaggcg acctgccaaa gcattggttg gaaccagcgc ccatctttcc
    15781 aggtctgata gtcttcctcg cgcggctcga caaagcccca atgctcgctg aatgcctcgt
    15841 tggagcggtc gtagaccttg cggtattcgc tcggaaaggc tggccgcacc tctatcccct
    15901 ccggcagatc aacatccgga agctcgttga ggtcgcggga catgctgaaa taccagcgta
    15961 cagctttgta accaaagctc tccagcaagc ccatacggtg tgtatcttcg ctggcggagc
    16021 actgtatgaa tactttctcg ccctcatccg caaggtcagc cgcgatttcg agctggcgtt
    16081 gttccatcca agtttgcatc gggatgtcaa ttccagaccc ttgcagcgct ggattcatgt
    16141 tgaaaatctg gtaaaaggct ttgcgagggc cttcctgcag ccacataacg cgcccgtaag
    16201 cagctgggac gccgtccatc tcagcaaaaa tcatatccct gaaagggtcg cagttgtaga
    16261 gatggcggaa gttaatctcg gcgtcctcca gggtgaatga gccttccgtg aggtctgccg
    16321 cgcgcatctg attccagatt tgcaccatga caggaaaatc ctcttctccg cggtagccgc
    16381 ggtagactac tcccggtaag tcgggtttaa tcatcgggtt gaaatttgcg ttcattcttc
    16441 agttccttct tttccttatt cacaccaaac gtcgttctca aacagtgaca gcgggaccgg
    16501 tttcccaggc tttttgataa caaaaaacca cggggtcggt ggacgcccgt ggttgtcttc
    16561 gcgaagtgca gaggcttaac gacttacagg cgcagtacgg ccttgaacag gaacgccggc
    16621 gcgaagccgg gtttcttctt gagctttatt cgggctgact gcgaccataa aaaaacctct
    16681 ccctgcatat tttagcacag gcatttctgg aacgtcaaga aaaaagtaga tgaaaaaagt
    16741 agatgaaatc gcaaaaaaga aatgtcctca ttaagctttg gcgctatgaa agcagcaatc
    16801 gattcctcca ggttttttta caaagcgatg agaatactct gacatacaag ggaataacag
    16861 cctttccccg tttttccctt ccccagcggc ttgaccgggg aaaaaaagag ccgcttgccg
    16921 ctggggtgag ggtaaagggg taacattatc gaaaacaaca aataaggggc ttagtttcgg
    16981 ggaaaaatcc gtcctgacga ctttctccac gaatcaggac atttcgattt aggcttgata
    17041 aagaagaatg ggggaatttc acgcgcggcg gctgcctgat gaatccccat actgcgagtt
    17101 cgcatcgtaa tgcgtgttaa aaagcccgac ggcaacggga agcctgccct cagggaaaaa
    17161 ttggcgttta tttggctttt ttcaggaaac cgaacaccag cacaaccagc accccaacgg
    17221 cgctcaggcc ggccaaaacc aacccgacag tctttccatc tgcgaaggcg cgctgaaaaa
    17281 caggcggtac cggcgtttcg gtcgggatgg gttggggtgt gtgcgacggc acgggagtga
    17341 gggatgggcg cggctgagtg ggctgcggag taatgcgcgt aggtgtaggg gtcagggtca
    17401 gcgtggcagt ggccgggttc ggcaggcgaa taatcaggat ttgaccagca tagacgtcgg
    17461 tggccagcgg gctgttgccg ctgttggtca tgatttccgc tccggtcagc ccgtatgcca
    17521 tggcaatcgc ccacagcgtt tccccttcgc ggacgatgtg gataatggag ccgtccgcgt
    17581 tgggggtgga ggtgatgatg gtgttgacat aggagctggt ggcttcctgt tgggggttga
    17641 gcgctgccgg ggcttgcgag ccgcgtccgg gcgagagcgc tttcgctgcc atgggaggaa
    17701 cgaccgcgag cgccaggaga aggcagaggc aaaggacaat tatttttttc atgatggcat
    17761 attatagctg ttttaccgaa gtcctgcctg aaaggctgtg ccaaatccac cctccactat
    17821 gccgctctct ggtctccagc ccattccatt ggtagaataa taggcatcac gctggtgcag
    17881 tttcccggcg tttgaggaga aaattatgct ggatttgaaa ttaatacgcg agcaacctga
    17941 gcttattcgt gaatccctgc gcaaacgcca tatggactcc ggtattgtgg accaaatcat
    18001 agggcaggac gaactgcgcc gcgcgctcat tctggaagtg gaagccaaaa aagccgaacg
    18061 caacgcggcc agcaaggaaa tcggtcggat gaaagacgct gccgcgcgcc aggcgaaaat
    18121 tgacgccatg cgcgagctgg gcgataggat ttctgccctg gacgagaaac tgaaatcggt
    18181 cgaagccgaa ttgcatctga gcgtggccgg cctgccgaat atccccgacc cggatgtgcc
    18241 tgtcgggctg gacgacaaag ataatatcgt cgtacgcacc atcggagaaa aacccgtcta
    18301 tgattttgaa ccgttggcac actgggacct gggtccgcgc cttgggatga ttaatttcga
    18361 agccggcgtc aggctggcgg gaacgcgctt ttacgtgttg gaaggcgcgg gggcgaggct
    18421 ggaacgagcg ctgattttct ggatgctgga tttgcacatc cgtcagggct accgcgagat
    18481 ttacccgccc tggatggtgc gccaggaggt gatgtttgcc tcgggtcagc tccccaaatt
    18541 cgcggataac ctctaccacg acgcggagga agatttttgg atggtcccga ccgcggaagt
    18601 gccgctgacg ggcatgatga tgggggaagt gctggacgaa gctactctgc cgctgaacct
    18661 gaccgcctac actgcctgct tccggcgcga gaagatgagc gccgggcggg atgtgcgcgg
    18721 aatcaagcgc gggcatcagt ttgataaggt ggagatgtac aagtactgcc agccaaagga
    18781 ttcgcccgct cagcttgacc agatggtggc ggatgcagag gaaacctgcc ggcagttggg
    18841 cctcacctac cgcgtgctgc tgcagtccac cggcgatttg agctttggct cgcataaaac
    18901 ctacgacctg gaagtgtggg cgccgggctg ccaggagtgg ctggaagttt catccgtttc
    18961 caacatcggg gaattccagg cgcgccgagc gaacattaag tatcgcccgg cagacggctc
    19021 ctcgcccagg tatctgcaca cgctcaacgg ctcgggcttg gggctgccgc gcacgctgat
    19081 tgccgtgatg gagacctatc agcaggcgga tgggagcatc cgcgtgccgg aaccgctgct
    19141 gccctttatg ggcgggatgg aagtcatccg cgcatgagca cttcccccgc ctgggtcgga
    19201 acgctaattc ttatccttac cgcactgttc caactcatcg gcctggtcgg gctggtgttg
    19261 gtattctttc cagggccgac cgtggcgtgg gttgggcagc tcatctgggc gatcttcacg
    19321 cgcttcaatt atgggcacga gccctggcag ttcacgctga ccattgtgtt tttcgtcatc
    19381 agcacgctgc tgatgctcgt gggttcgttc ctggacaacc tgatgatggc ggggcgcgcc
    19441 cgaaagcagg gttcgccctg gtgggagatt gggctgagct ggttcgcgat gattgtgggg
    19501 ggaattctgc tgacgccgct gggtggactg gccgccgcac tgttgacgtt attcctggtg
    19561 gaataccagc ggttggggaa gaagcagaag gaagcctggg ccgcgacgaa ggcggtggcg
    19621 ttgggttggg gttggtccac gctaatcaga gttgggattg tgctggtgat gattgcgctc
    19681 tggctggtat cggtagtgtg gttttaggga tttgacattc gtcaaggtaa gcggttttgt
    19741 ttgtcatcct gagcgcagcg aaggatctat cagttaattc gtcatcgcga gcgcagcgtg
    19801 gcgatctgct tccatattga tcaggtttgt aggtcgggtg gagcggcagc gcaacccgac
    19861 atgctttggc cggcgggttt gtggagccgt cgttgggttt cgctcctgcg ccgcggtttg
    19921 taggtcgggt ggagcggcag cgcaacccga catgctttgg ccggcgggtn nnnnnnnnnn
    19981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt agtggagtcg tcgttgggtt tcgctcctgc
    20101 gtcgctgcac ccaacctaca agctttggcc ggcg
//
LOCUS       DAOQAD010000027        19791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_2851_length_19791_cov_5.435803, whole genome shotgun sequence.
ACCESSION   DAOQAD010000027 DAOQAD010000000
VERSION     DAOQAD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19791
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2851_length_19791_cov_5.435803"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            762..2759
                     /locus_tag="PKL60_03700"
     CDS             762..2759
                     /locus_tag="PKL60_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNW95273.1"
                     /translation="MPFLTKLLIALGVLAVLTLISGIRFIPNNRIGIVEKRFGRRSLK
                     SGFIALHGEAGYQPNVLRGGLHFLMPIQYRVHIAPLVTIAQGKIGYVFARDGEPLSAM
                     QVLASNTTANNFQDVTHFLTNGGQRGPQRQILREGTYAVNLAQFVVITEERVYYLALS
                     RDDQIVIENMTSVIKERRGFTPVVIKDQDDLIGIVTVHDGPSLPSGEIIAPIVGSDLN
                     DSATYHNNFQMPDRFIAASGLRGRQLQVLVEGTYYLNRLFATVEMIHKTIVEVGFVGV
                     VVSYTGKVSEDLSGLDYRHGELVSKGSRGVWSDPLLPGKYAFNTYAGKVVMVPTTNII
                     LKWIKSEVGSHNLDENLSEVSLITKDAFEPSLPLSVVIHIDYQKAPLVVQRFGDVKKL
                     VEQTLDPMVSAYFKNIGQTRTLIELIHERNEIQRQSSQEMKDKFLHYNLELEEVLIGT
                     PTTSGEDKNMEIILNQLRARQIAVEQIETFQRQQVAAQKERELREAQARADQQRNVTE
                     SEVSITVLSNQGKADYARAVQQAAQIRTLAEAEAEKIARTGIAQAIATEEQVAAYGGP
                     QFQVTQQVMNRFAEAVQQSRVDVVPRVVVGASGSEGGATGSSIMEGLLTLLLSDKLNL
                     EVSAAAQKPRSEETEQLKAEIKRSLTEKKAPAEGQAPDTKG"
     gene            3457..4092
                     /locus_tag="PKL60_03705"
     CDS             3457..4092
                     /locus_tag="PKL60_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNW95274.1"
                     /translation="MAQEEVIIKAENRTVKGKQVKALRRAGYLPGIIYGRHLEALPIQ
                     MNAHEASLKLSKLTSSSLITIDVDGTTHAVIVRDRQRDPIYGRLIHIDFLAVSLTEIL
                     RTSVEIGLVGEAPVSKLADVVILHNLYELEIECLPKDLPSKIEVDLSGLKSVDDSITV
                     ADLKLGDKITVLTDPEEVIVSVSYAEQEAPAEGEAEAEPDVVEKGKKETEE"
     gene            complement(4148..4747)
                     /locus_tag="PKL60_03710"
     CDS             complement(4148..4747)
                     /locus_tag="PKL60_03710"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNW95275.1"
                     /translation="MKELTIEPDVLARKLAALRGKPHAEYESDSSFDPREYKPAAVLV
                     PLLWYEGEWHLLFTRRSSSLMNHGGQVAFPGGSWDVEDEDLIATARREAWEEVGLRPE
                     DVRVLGDMSAIGIITRFLVTPVVGIIPWPYPLRLQEAEVARAFIVPLSWLANPGNFYT
                     STRIYEGKSYEIGYYQLYDGEKIWGATAQMTREFLKLIN"
     gene            complement(4744..5559)
                     /locus_tag="PKL60_03715"
     CDS             complement(4744..5559)
                     /locus_tag="PKL60_03715"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNW95276.1"
                     /translation="MSALAQDGDLVQLQGAGYKSHILRLKAGEVLQTHRGVIRHDAVI
                     GQPWGARLQSHTGSPFYLFQPGLADLIRETKRTTQILYPKDIGYILLVMGVGPGKRVL
                     EAGTGSGGLTTAFAYMVGETGKVISYERKPEVQALARSNLSRVGFENRVDFKLGDAAE
                     GFAERDVDALFLDLPNPYDYIGQAKASLKGGGYLGILVPTVNQVSRCLQTLKNQQFAF
                     VEVCEILLRYFKTDWERLRPTDRMVAHTGFLIFGRSVFETSDELFDGEVSEPA"
     gene            complement(5556..5822)
                     /locus_tag="PKL60_03720"
     CDS             complement(5556..5822)
                     /locus_tag="PKL60_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95277.1"
                     /translation="MILSLVLYGFLFATLAAALFHLWRDGGLGKLILYLLASWAGFWL
                     GHLAANALDLHILDVGELHLGPGLLGSLIFLFLAHWFSQIGKPV"
     gene            6072..6899
                     /locus_tag="PKL60_03725"
     CDS             6072..6899
                     /locus_tag="PKL60_03725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNW95278.1"
                     /translation="MFADTHCHLDFDAYDADRAEVILRARQAGLAFLVNPSVDLPSAQ
                     AVRKLTADYPGYLFAAVGIHPNFSAGCEKDQISRLEELAAGPEVVAIGEIGLDYYRDY
                     SPPAQQRCSFLWQLELAALLELPVIIHDRDASQDLLPILKDWHGALPPQTPIKRRPGV
                     MHAYSGSAEQARALAELGFCFGLGGPVTYQNAQERRWVAAQIPLGNILLETDAPFLTP
                     HPHRGKRNEPAYIPLIAETIAALHGISAAELGEITTANAAGLFGLRDKPETSFRAAA"
     gene            complement(6896..7801)
                     /gene="ppk2"
                     /locus_tag="PKL60_03730"
     CDS             complement(6896..7801)
                     /gene="ppk2"
                     /locus_tag="PKL60_03730"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNW95279.1"
                     /translation="MAKHNGKSEEKGKDKKSAKEKPEGKTEQTPAVSIPQGKLSTKEY
                     ERELEKLQVELVKMQGWIKEKGLKVVVLFEGRDAAGKGGTIKRITEKLNPRIVRIVAL
                     ATPTEREKSQWYFQRYVQHLPAAGEMVLFDRSWYNRAGVERVMGFCTEEEVREFFRSV
                     PEFENMLIRSGIILIKYWFSVSNEEQERRFQARINDITRRWKLSPMDLASRTKWVEYS
                     KAKDEMFRYTDTKQSPWYVVNSDNKELARLSCISHLLSKIPYEDLTPEPIVLPPRQAD
                     DNYVRPPMNTQTFVPEYKLTEKEKA"
     gene            complement(8106..8840)
                     /locus_tag="PKL60_03735"
     CDS             complement(8106..8840)
                     /locus_tag="PKL60_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW95280.1"
                     /translation="MDATPKKNRILVVDDEKGLVKLIRLNLEHDGFEVFEANNGAQAM
                     DRLRAVLPDLVLLDVMMPDLDGFQVLRMIREVVSTPVIMLTAKGEENDKVRGLELGAD
                     DYVTKPFSPRELTSRIRAVLRRGSFSDQTDTGKIDVDGRLQIDFDRHEVWVDGALVQL
                     RPTEYRLLYHLVKNAGWVLTHDQILSKVWGYEYEDEPHYVRLYINYLRKKIEKDPANP
                     RYILTERGVGYRFVDYRRENLAGKAD"
     gene            complement(8856..>9662)
                     /locus_tag="PKL60_03740"
     CDS             complement(8856..>9662)
                     /locus_tag="PKL60_03740"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNW95281.1"
                     /translation="DPGAKTERKRSSLPVIESHAEVSIGKVRKQNEDVVFAVHARSLR
                     PELQVSYGLFVVADGMGGHENGEVASSTAVAALVNFLRQQLFPRLQQGSGLPSTPEVQ
                     EILQSAAQAAQEAVLSAVPGGGTTLSAALVINSGLYFAHVGDSRIYHYTPRAGLETLT
                     RDHSVVRRLVDLGQITAEEARSSPLRNQLFRAVGQGDSFKADLGFRELKEPCTLLLCS
                     DGLWGLVPEEKILKTLEKGQSAQASVQSLITMANKAGGSDNISVLVVKIS"
     assembly_gap    9663..9672
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9781..10284)
                     /locus_tag="PKL60_03745"
     CDS             complement(9781..10284)
                     /locus_tag="PKL60_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95282.1"
                     /translation="METKPTRSGIHPLSILAAIFLAAAILGLVYLLFRLTRPETPTQQ
                     EIPTAALTMIPAPTHTPTLPATLEPAAATQTAAPLLPPGTIGVGAYVKVTGTGGAGLR
                     MRAEPGTDADIHFMAMDEEVFLVIGGPVEKDGYVWWQLKASYDANRTGWSAESFLSLV
                     ETATPTP"
     gene            complement(10304..11341)
                     /locus_tag="PKL60_03750"
     CDS             complement(10304..11341)
                     /locus_tag="PKL60_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNW95283.1"
                     /translation="MKEKRKKKRGFLPRGISGLVTAGVLTSAAWILYSRRYIDHHARV
                     KGVLEAEQGSFEAPTAGKLHFTADTRSSGRPLVILHGIHAAAGSHDIAPIFNAFRSQR
                     PVYALDLPGFGGSERSDRPYRPSMYQAALTEFIREQVGEPADVIAQGLSAEFAALAAQ
                     ANPGLIRSLILLSPTGFEMPQVGSLSEKIGKANPADLLYTLLAVPLWSLPLFDILSSR
                     PRLSLYYRQRFEADVPEELVSIAYASAHQPGAHFAPMVFASGRLSVRDVREKVYDTLT
                     QAVLVVYDSEPGRSFAMLLPTARAHANWSVRRIRQSRGMPHFDRPGEFFHVLEDFLKA
                     QDKRGAEENRG"
     gene            11461..12978
                     /locus_tag="PKL60_03755"
     CDS             11461..12978
                     /locus_tag="PKL60_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNW95284.1"
                     /translation="MKTKTRRSRVDPLRWLIGGFGLFFLLFSLYILLKDYTAYRASSR
                     LYPGGVSVAQIPIGGLTREQAEQRLTEAFSAPVELRYSGARMQFAPAELGFSLNAAAS
                     LDQVDQQIPRGAWWRHLWGQSSPAYTQDFPLQASLDAAAARAFLVNTVQPRYDQAASE
                     ALPILYSTNYTLGQSGYALDLNGALAALEPALFSASDRTVDLPIRETPAPGLNPRNLE
                     VFLRQTILLDGFDGLVEIYLQPLGGQEALHFAVRDNQTVAPDVAYSAASTIKIPIMSS
                     VLKHLEEPTPDTARYWMERMIIHSENPPSDALMRTYLDENLGPLIVTEDMRTLGYQNT
                     FLAGYFYAGAPLLQRFITPANSRTDVYLDPDFYNQTVPSEIGDLLARIYRCAHAASAQ
                     DALFDGQVSQAECQTMLDLLGQNKIGALIEAGLPPQASVAHKHGWTSDLDGLLHTMSD
                     AGLVSTPGGDYVLIIFVNSEKQLVFDEGNWLFARLSQVIYNAYNLQQQAAWLPGN"
     gene            13016..15670
                     /locus_tag="PKL60_03760"
     CDS             13016..15670
                     /locus_tag="PKL60_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNW95285.1"
                     /translation="MRVILTHEQADLDALASLLGAHLLDKDAYALLPRQINRNGQAFL
                     QKFQQELGFNTVSDLPGESITQVTLVDTQSLITLKGFSARTHVSVIDHHPRKAHTDPE
                     WAVELHETGACTTVLVEKLQKAQTPFSASQATLLLLGIYEDTGSLSYAMTTPRDLLAA
                     AFLLEHGADLSTASRYLNPPLSNAQMLLYDRLMKDITAHKIHDFTILVAKANALDIQD
                     EISTVAHHMREFLNPDGLVLLVSTRQGIRLVARSTTDEIDMAQLAHRFGGGGHKRAAS
                     ALIRSESRPTPNEVPVFWKKTYQYVVEMLPEIVHPSMRVQQIMSRKPLLVSPDMPVET
                     VADLMKRYGFEGYPVIEEGKVVGLITRRNVDRALSHRMMANAGILMDAGTVYVTPGDT
                     LDYLQEVMASSGWGQVPVLDPESGEIIGIVTRTDLINTHALQNNIPNQDEMVRRLHNA
                     IPYPRQILLQAVARLAGKLNMQAYIVGGFVRDLLLKQPSQDFDIVVEGDANVFVAKLV
                     EIYGGRAVTHSRFGTAKWIIGDEREALAQKLLPDQPMDFQALPQHLDLITARTEFYER
                     PAALPTVERSGIKMDLHRRDFTINTMALRLDAPYYGKLYDFWGGYHDLQNHIIRVLHA
                     LSFVDDATRILRAIRFSTRFDFRMDPRTLSLLSSSLSLLNEISGSRLRHELDLILLEF
                     NVGTALRAMDRLSVLEVINPNLNWNPALEARIAFLNQPAAQAEWKTETHENQLNLRQV
                     LGYCYWLEDLSPEQLEQVSARLRIPARVTSAISDTAKLRRLLPELGALKPSQVVNALK
                     GISPLALTAVYQSTEDPALREPLRLYQTRYLDVKPLHSGDDLRARGLPPSPEFQRILN
                     ALRDAWLDGSVSSPEEEDALLEQLLNAT"
     gene            15685..16212
                     /locus_tag="PKL60_03765"
     CDS             15685..16212
                     /locus_tag="PKL60_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW95286.1"
                     /translation="MASTFQLPDGEEILIRPIREADLPALEWEGEYRHFRKLYAEHFE
                     AFRAGTALIYVAETRAGVMIGQIFIQLYSRNKEAADGIHRAYLFSFRIKPSHRSMGLG
                     TYMLRFVEDQLLMRGYTTIRLNVAQDNTRARALYERCGYQVIGYSSGVWQYQDDQGEW
                     QTMHEPAWKMSKRLR"
     gene            complement(16319..16837)
                     /locus_tag="PKL60_03770"
     CDS             complement(16319..16837)
                     /locus_tag="PKL60_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95287.1"
                     /translation="MKTYKLDPAKLPAQKKTLILVYGITFLVMAALSVGINWNRESMK
                     SLVWMIPLIAALFVYSGWRAYQQRKSLWEEYTFTVTEDGLVLHQPKYPDLKLSKTDIT
                     GIEDVKNGTYVSTTRGRRLFAITKLFPDADYAEMRATMLRWIDENKASKPAEEESAAE
                     PAEEEDLPEQEE"
     gene            17418..19484
                     /gene="fusA"
                     /locus_tag="PKL60_03775"
     CDS             17418..19484
                     /gene="fusA"
                     /locus_tag="PKL60_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNW95288.1"
                     /translation="MKEFSTASVRNIALASHSNSGKTMLTEAFLHFTGATTRLGKIED
                     GTTVSDFDDEEHRRGISLFTSVIPVLYKDLKFNFLDTPGYSDFVGEVVSAMRVADSVL
                     ILVDSVGGMEVGTETAMNYAAKFKLPMFMVINKMDRENASFQKALASVQEQTDYRLIP
                     MQLPWGEKADFKGVIDLISMKAFQGEGKTAVEIPAEFLPEAEEARLKLIEAAAEGEDS
                     LLEKYFENGDLTTEEALQGLHRAVQISNVIPVMVAAGGHMVGVAPLLEALSTLAPSPL
                     EHADVVAKSPKGDVQLTPSDSGPLAAYVWKTTADPFVGKQTFFRVFSGVMSSDTRVWN
                     ANKGEEERLAGMQVPFGKENLPVSVIHSGDIGTVAKLSLTTTGDTLTTKEQSLILPMP
                     EYPSPLYRVAVSPKTQSDAAKMSTTLSRLCEEDLTLTWHMEPATRQTLLFGMGDQHID
                     VAVRRAEQKFQMSMNLLEPKVPYEEHITKEASAMYRHKKQTGGSGQFGEVHLKVFPIK
                     DEDFSFSNDVFGGAISNSYMAPIEKGIRAVMKDGVVAGYPVHNVAVSVFDGKEHPVDS
                     KPIAFEIAGREAFKLAFKDANPVLYEPIMKVTVVVPEANMGDVLGDMNTRRARVQGMD
                     TEKGRSVVTVTVPLAEMLRYTTTLRSMTGGRGYFSMEFDHYDLVPSHLAQEIIDARQK
                     ELKEEE"
BASE COUNT         4322 a         5991 c         5368 g         4100 t
ORIGIN      
        1 catgtgggca gaccgggcga ggttcctgac cgcaggtctc cgcagtcgta ggggcgaagc
       61 atcgtttgtg accttcccct gggggaaggc gcccgcaagg cggatgaagg tcttttcctg
      121 accttcatca atctcgctgc gctcgcccgc ttcgccctgg agaagctttt tgtaggccgg
      181 cttgcgtttt tcaagctgcc ggctttgtca ggttgacaaa gtgctgcgaa gcagaaagac
      241 cgcaacctga cctacgaaca actggcaatt cattcgtcat cgcgagcgca gcgcggcgat
      301 ctccctattg tcacaaagtg atcgtaaatt caatcctcag ggcggatgaa ggtctttgac
      361 taaccctcat cagtctcgct gcgctcgccc gcttcgccct ggagaagctt tttgtaggcc
      421 ggcttgcgtt tttcaagctg ccggctttgt caggttgaca aagtgctgcg aagcagaaag
      481 accgcaacct gacctacgaa caactggcaa ttcattcgtc atcgcgagcg cagcgcggcg
      541 atctccctat tgtcacatag tgatcgtaaa attcaatcct cagggcggat gagagtcttt
      601 gactaaccct catcagtcct gctgtgctcg cccgctttgc ctccggcatg cgagcagccc
      661 caaaacaatc cgccaaagcc tatattatct atggtatttc cctgtgcgcg ggcgtatact
      721 tctggcgttt ccaacattca cgagactcca aaggagttca tatgccattc ctcacgaaac
      781 tacttatcgc tttgggcgtc cttgcggtgc tgaccttgat ttccggaatc cgcttcatcc
      841 ccaacaaccg cattggaatt gtggaaaagc gcttcgggcg ccgctcgctt aaatccggct
      901 tcatcgccct gcacggcgaa gcgggctacc aacccaacgt cctgcgcggc ggcctgcact
      961 tcctgatgcc catccaatat cgggtgcaca tcgccccgct ggtgaccatc gcccagggca
     1021 aaatcggtta cgtcttcgct cgcgacggcg aaccgctcag cgccatgcag gtgctggcct
     1081 ccaacacgac cgccaataac ttccaggacg tgacgcactt cctgacgaac ggcgggcagc
     1141 gcggtccaca gcggcaaatc ctgcgtgagg gtacctacgc ggttaacctg gcgcagttcg
     1201 tggtcatcac cgaagagcgc gtctactacc tggctctcag ccgcgatgac cagatagtta
     1261 tcgagaacat gacctcggtc atcaaagaac gcagaggctt tacgcccgtc gtcatcaaag
     1321 accaggacga cctgatcggc attgtgaccg tgcacgacgg cccctctctg ccctcgggcg
     1381 agattatcgc gcccattgtc ggctccgacc tgaacgacag tgccacctat cacaacaatt
     1441 tccagatgcc cgaccgcttc atcgctgctt ccggtctgcg cggccggcag ctgcaggtat
     1501 tggtggaagg cacctactac ctcaaccgcc tgtttgccac cgtggagatg atacacaaaa
     1561 ccatcgttga agtcggtttt gtaggcgtgg tggtttctta caccggcaaa gtcagcgaag
     1621 acctctccgg tctggattac cgccacggcg agctggtcag caaaggcagc cgcggcgtgt
     1681 ggagcgaccc actcctgcct ggcaagtacg ccttcaacac ctatgccggc aaagtggtga
     1741 tggttccgac caccaacatc atcctcaagt ggattaagtc cgaggtcggc tcgcacaacc
     1801 tggacgagaa cctctccgag gtctcgctca tcaccaaaga tgccttcgag ccttccctac
     1861 cgctttcggt ggtcatccat atcgactacc agaaagcccc gttggtggtg cagcgctttg
     1921 gcgacgtgaa gaagctggtg gagcagaccc tcgacccgat ggtttccgcc tacttcaaga
     1981 acatcggcca gacccgcacg ctgattgagc tcatccacga acgcaacgaa atccagcgcc
     2041 agtccagcca ggaaatgaaa gataaattcc tgcattacaa cctggaatta gaggaagtgc
     2101 tgattggcac gccgaccacg tcgggtgagg ataagaatat ggaaatcatc ctcaaccagc
     2161 tgcgcgcgcg ccaaatcgcg gtggagcaaa tcgaaacctt ccagcgccag caagttgccg
     2221 cgcagaaaga acgcgaattg cgcgaagccc aggcgcgcgc cgaccagcag cgcaacgtga
     2281 ccgagtccga ggtgagcatc acggtgctct ccaaccaagg caaggccgat tacgcccgcg
     2341 ccgtccagca agccgcccaa atccgcacgc tggcggaggc ggaagctgag aaaatcgccc
     2401 gcaccggcat cgcccaggct atcgccaccg aagaacaggt tgccgcgtac ggcggcccgc
     2461 agttccaggt gacccagcag gtcatgaacc gctttgccga agccgtgcag cagtcccgcg
     2521 tggacgtggt gccgagagtg gtggtgggcg cttctggcag tgaaggcggc gcgaccggca
     2581 gcagcatcat ggaaggcctg ctcacgctcc tgctctccga caagctgaac ctggaagtga
     2641 gcgccgccgc gcagaagccg cgcagcgaag agaccgagca gttgaaagcg gaaatcaagc
     2701 gcagcctgac cgaaaagaaa gcgcccgcgg aaggccaggc gccagacaca aagggctaaa
     2761 ccccagtgat tagcagaaaa aggattggtt ttcgaaccaa tccttttttt tgatttatac
     2821 cggcacttcg ccctttctca aaagtcccca caggcggatg cccgcgattc agggcagcgc
     2881 gagcgccgcg cattagagca gcagcccgac caccacgttc atctgcccag tgccctcgcc
     2941 cacccggccg gaggccagcc ccaaccagac agccgccaat tggctgaaca caaggaagaa
     3001 gaccgccgcc gcgaaagacc aaatgaccag tttttaccgt tttttgcgcg gagagccgca
     3061 caaatcagca gcgctccccc aaccaggcag accggcgcca gttcagcggg cagcaggtaa
     3121 ttcagcagga gcttacggct gccaggcagt aaactgaacg cgagcgcgaa cggagccaat
     3181 accggtactc acaccagggc gcaaccgacc acaatcagta tcctggtaaa cacagaacgg
     3241 gctttcatag gtcctccaat ccatagatga gaaccttaaa acttgtaccc cagcgctgag
     3301 aaccaggggg cgcttttcgg ccgacctgtg ttgggccgct gcttgacgca gacccataaa
     3361 agcgataaac ttttcagtcg ccggaaattc gtccggtaac tacaaggcga gggcagcccc
     3421 aggcggggtc agaggcgccg aaaaggaaaa attagcatgg cacaagaaga agtaatcatc
     3481 aaagcagaaa acagaaccgt caagggcaaa caggtcaaag ccctgcggcg cgccggatat
     3541 cttcccggca tcatctacgg ccgtcacctg gaagctctcc caatccagat gaacgcgcac
     3601 gaagcttcgc tcaaactgag caaactgacc tcctccagcc tgatcaccat cgatgttgac
     3661 ggcacgaccc atgcagtcat cgtgcgcgac cgccagcgcg accctatcta cgggcgcctt
     3721 attcatatcg acttcctggc ggtttccctg accgaaattc tgcgcaccag cgttgaaatt
     3781 ggtctggtcg gcgaggctcc ggtctcgaag ctggcagatg tggttatcct gcacaacctc
     3841 tacgaactgg aaatcgaatg tctgccgaaa gacctcccct ccaaaatcga ggtggatctt
     3901 agcggtctga agagcgtgga tgacagcatc accgtcgccg acctcaagct tggggacaaa
     3961 atcacagtgc tgaccgatcc ggaagaggtg attgtttctg tcagctacgc agagcaggaa
     4021 gcccccgctg aaggcgaagc tgaggcagag cccgatgtgg ttgaaaaagg caagaaagag
     4081 acagaagaat aatttgcttc tgccagtgtc ggatacacac aaggctgccg ttctatggca
     4141 gccttgttca gttaatcagc ttaaggaatt ccctggtcat ctgcgcggta gcgccccaga
     4201 ttttttcgcc gtcataaagc tgatagtagc caatctcgta gcttttgcct tcatagatgc
     4261 gggtgctggt atagaagttg ccggggtttg ccagccacga aagcggcacg ataaacgccc
     4321 gcgccacttc cgcttcttgc agtcgcaatg gatacggcca gggaataatg ccgaccaccg
     4381 gcgtaaccag aaaacgcgta ataatgccaa tcgcggacat gtcgcccaac acgcgcacgt
     4441 cctccgggcg caagcctacc tcttcccacg cttcgcgccg cgcggtggca atcaaatcct
     4501 catcttccac gtcccagctt ccgccaggaa aagcgacctg acctccgtgg ttcatcaggc
     4561 tgctgcttct gcgcgtgaag agcaggtgcc actccccctc gtaccaaagc aaagggacca
     4621 gcaccgcggc aggtttgtac tccctggggt caaaactgct gtcggattca tattccgcgt
     4681 gcggtttgcc ccgcaaagca gccagcttac gcgccagcac atccggttcg atcgtcaatt
     4741 ctttcacgct ggctcactga cctccccgtc aaagagctca tcgctggttt caaacacaga
     4801 gcgtccaaaa atcaggaagc cagtatgggc gaccatccga tccgtcgggc gcagcctttc
     4861 ccaatcggtc ttaaaatacc gcagcaggat ttcacaaact tccacgaagg caaactgttg
     4921 attctttagc gtttgcaggc agcgggaaac ctggttgaca gtcggcacga ggatgcccag
     4981 gtaaccgccg cccttcaggc tggcttttgc ctgcccgatg taatcgtacg gatttggcaa
     5041 atccagaaag agggcgtcca cgtcccgttc ggcaaatccc tccgcggcgt ctcccagttt
     5101 gaagtcgacc cggttttcga agcccacccg gctgaggttg gagcgcgcca gcgcttgcac
     5161 ttccggcttg cgttcgtaag atatcacctt acccgtttcc cccaccatat aggcgaaggc
     5221 ggtggtaagc ccgccggagc ccgttcccgc ttccaaaacg cgcttgccgg ggcctacgcc
     5281 catcaccagc aggatgtagc cgatatcttt cgggtagagg atttgggttg tgcgtttggt
     5341 ctcgcggatg agatccgcca ggccgggctg aaacaaataa aaggggctgc cggtgtgcga
     5401 ttgcaggcgc gccccccagg gttgtccgat tacggcgtcg tgccggatta ctccgcggtg
     5461 ggtctgcagc acctcgccgg ctttcaggcg caggatgtgg cttttataac cggcgccctg
     5521 cagctgcacc aggtcgccgt cctgagcgag agcgctcata cgggtttacc aatctggctg
     5581 aaccaatgcg caaggaacag gaatatgagg gagcccagca gcccggggcc gagatgcagc
     5641 tcgccaacgt ccaggatgtg caggtccaat gcgttggctg ccaggtgccc cagccagaag
     5701 cctgcccaac ttgcaagcag atacaggatt aactttccca gaccgccgtc ccgccagagg
     5761 tgaaacagag ccgccgccag cgtggcgaac agaaaaccat atagaacaag tgaaagaatc
     5821 atgcctatcc tcacaagagg ccgtcagcgc aaagggagcg tttcccccgg ataagcgcag
     5881 gcggacccca agtctgtagt attttaacct ttaaatcata acacacttgg cgcgctgcca
     5941 gatttttccg ggctcgagcg cgctcccagt ccacaggact gcgccgtgca aatactcccc
     6001 tcggattgtg cctatccttt tctgcggttt gccccggcct gaaggtataa tttctgccgg
     6061 aaggaatacg aatgttcgcc gatacgcact gccatctcga ttttgacgct tatgatgccg
     6121 accgcgcaga ggtcattctg cgcgcgcggc aggctggcct ggctttcctt gtcaatccct
     6181 ccgtggacct gccttccgcg caggcagtgc ggaagctgac cgcggactac cccggctatc
     6241 tcttcgcggc agttggcatc caccctaatt tcagcgccgg ctgcgagaag gaccaaatca
     6301 gccgcctgga ggagttagct gccgggccgg aggtcgttgc catcggggaa attggtttgg
     6361 attactaccg ggactacagc ccgcccgcac aacagcgctg cagcttcctc tggcagttgg
     6421 agctggctgc ccttctggaa ctgcccgtca tcattcacga ccgggatgct tcgcaggact
     6481 tgctgccaat cctgaaggac tggcacggcg cgctgccgcc acagaccccc atcaagcggc
     6541 gtcctggtgt gatgcacgcc tacagcggca gcgcggagca agcccgggct ctggcggaac
     6601 tcggcttttg ttttggactc ggcggacctg tcacctatca gaacgcgcag gagcgccgtt
     6661 gggtggcagc acaaattccg cttgggaaca tcctgctgga aacggacgcg cccttcctca
     6721 ccccacaccc ccacaggggg aaacgcaacg aacccgccta catccccctc atcgcggaaa
     6781 ccatagcggc gctgcacggc atcagcgcag ccgaactggg cgaaatcacg acagcaaacg
     6841 cagccgggtt atttggtctg agagacaagc ccgaaaccag cttccgggcg gcagcttagg
     6901 ccttttcttt ttccgtcagt ttatattccg gcacaaaagt ttgcgtgttc atcggcgggc
     6961 gcacgtaatt atcatccgcc tgccgcgggg gcagcacaat cggctccggg gtcaggtctt
     7021 cgtatggaat cttagaaagc aggtggctga tgcagctcag acgtgccagc tccttattat
     7081 ccgagttgac cacataccag ggcgattgtt tggtgtcggt gtagcggaac atctcgtcct
     7141 tggccttgga gtattccacc cattttgtcc gggatgccaa atccatcggg ctcagtttcc
     7201 agcgccgggt aatatcattg atgcgcgcct ggaaacgacg ttcctgttcc tcgttgctga
     7261 cggaaaacca gtacttaatc aggatgatac ctgagcggat gagcatattc tcgaactcag
     7321 gcaccgagcg gaagaattcg cgcacctctt cttctgtgca aaagcccatg acgcgctcca
     7381 cgccggcgcg gttgtaccaa ctgcggtcaa aaagaaccat ctcaccggct gccggcaggt
     7441 gctgtacgta gcgctggaag taccactgcg atttttcccg ttcagtcggc gttgccagcg
     7501 caacaatgcg cacaatgcgg gggttcagct tctcggtgat gcgcttgatg gtgccgccct
     7561 tgcctgccgc gtcgcgcccc tcgaacagca cgaccacctt gagccctttt tccttaatcc
     7621 agccttgcat cttgaccagc tctacctgca gcttttcgag ctccctctca tactcttttg
     7681 tggaaagctt cccctgcgga atggacactg ccggggtttg ttcagttttt ccttccggtt
     7741 tttccttcgc ggattttttg tctttcccct tttcctctga tttcccatta tgttttgcca
     7801 tagaagcctc tccttaccaa gaaacggaac aaatatgccg aaaaatcata tggatgatgt
     7861 taatcgctgt gtaaataatt atacataggt tttgcggccg cgccggcaaa aataagaact
     7921 gaaaaacgcg gcggcaacca aaaaaactta gcttgtggga acccccgttt tggaaacgac
     7981 ctgaaacgcg aaccaagtcg aaggggacgc agccaccaag gatcgattcc aggcatgcag
     8041 ccccaaggtc cagacacaaa tgatccgcta aatttaatct ttacccagcc tatgtccgct
     8101 agcgctcaat cggccttccc cgccaggttt tcgcgccggt aatccacaaa gcggtacccc
     8161 acgcccctct ccgtcagaat atagcgcgga ttagcagggt ccttttcaat ctttttccgc
     8221 aggtaattga tgtacaggcg cacgtaatgg ggttcgtcct cgtactcgta gccccacacc
     8281 ttgctcagaa tttggtcgtg agtgagcacc caaccggcgt ttttcaccag gtggtaaagc
     8341 aagcggtatt cggtcggacg cagctgcacc agcgcgccgt ccacccagac ctcgtggcgg
     8401 tcaaaatcaa tctgaaggcg cccgtccacg tcaatcttgc ctgtgtcggt ctggtcggaa
     8461 aagctgccgc ggcgcagcac cgcgcggatg cggctggtca actcgcgcgg gctgaaaggc
     8521 ttggtgacgt aatcatcagc gcccagttca agtccgcgca ccttgtcgtt ctcctcaccc
     8581 ttggcggtga gcattatcac gggcgtgctg acgacctccc gaatcatgcg cagcacctga
     8641 aagccgtcca aatcaggcat catcacgtcc agaagcacca ggtccggcag caccgcgcgc
     8701 agcctgtcca tcgcctgcgc gccgttattg gcttcaaaaa cctcgaatcc gtcatgctca
     8761 aggttgagcc ggatgagctt gaccagacct ttctcatcat caaccaccaa aatccggttt
     8821 tttttgggcg tcgcgtccat ttccttccct ttccttcaag aaattttcac tacaagtaca
     8881 gagatgttat cgctgccgcc ggctttgttt gccatcgtaa tcaggctttg cacgctggct
     8941 tgtgcggact gtcctttttc caaagtcttt aaaatttttt cctcaggtac cagcccccat
     9001 aatccatccg aacacagcag caaggtgcag ggctctttta gctcccggaa gccgaggtca
     9061 gctttgaagc tgtccccctg accgaccgcc cggaaaagct ggttgcgcag ggggctgctc
     9121 cgggcttctt ccgccgtaat ctggcccagg tcgaccaacc tgcgcacgac actgtggtcc
     9181 ctggtcaggg tttccagacc cgcgcgcggg gtgtagtggt aaatgcggct gtcgcccaca
     9241 tgcgcgaaat aaagcccgct gttgattacc agcgccgcac tcagggtggt tccaccaccc
     9301 ggaacggcgc taagcaccgc ttcctgggca gcttgcgccg cggattgtaa tatttcctgg
     9361 acttccggcg tggagggcag gcctgagccc tgctgaagtc ggggaaaaag ctgctgccgc
     9421 aagaaattta ccagcgctgc cactgccgtg ctgctggcga cctcaccgtt ttcgtgtccg
     9481 cccattccat ccgccacgac aaacagtccg tacgaaacct gaagttcagg cctcaggctg
     9541 cgcgcatgca cagcgaaaac gacgtcttcg ttctgtttcc gcactttgcc gatggacact
     9601 tcagcatggc tctcgatgac gggcagagag gacctcttcc tttctgtttt tgctcccggg
     9661 tcnnnnnnnn nnaaaaacac caaaaagccc tatgtggtga ccacgtttgt gttctttacg
     9721 ctcttctttt gctcaaaagc cgccactcta gaatactcct ctgctgtgcc gcagccgcgc
     9781 ttacggcgtt ggggtggctg tttccaccag gctcaaaaag gactcagcgg accagccggt
     9841 gcggttggcg tcataggatg ccttcaactg ccaccacacg taaccgtcct tttccaccgg
     9901 tccgccaata accagaaaga cctcttcatc catcgccata aaatggatat cggcgtcggt
     9961 gccgggttcc gcgcgcatgc gcaggcctgc gccgcctgtg ccggttacct ttacgtaagc
    10021 gccaacccca atcgttcctg gcggaagcaa cggtgcagcg gtttgcgtcg ccgcggctgg
    10081 ctccagggtg gcaggcagtg tgggagtatg ggtcggtgcg ggaatcatcg tcagcgcggc
    10141 agttgggatt tcctgctgcg ttggagtttc cggtcgggtc agacggaaca gcagataaac
    10201 cagaccaagg atggccgcag ccaaaaagat tgccgctaaa atcgaaagcg gatgaattcc
    10261 ggagcgggtt ggtttggttt ccatacggac ctcactttgc gggttatccc ctgttttcct
    10321 cagccccgcg tttatcctgc gcctttaaaa agtcctccag cacatggaag aattcaccgg
    10381 gacggtcaaa atgcggcatt cctcgcgact gccggatgcg ccggaccgac cagttggcat
    10441 gagcccgggc agtcgggagc agcatggcaa aacttctgcc cggttcactg tcatagacca
    10501 ccaacacagc ctgggtcagc gtgtcataca ctttttcccg cacatcgcgc acggataatc
    10561 tgccgctggc aaacaccatt ggcgcgaagt gcgcgccagg ctgatgtgcg ctggcgtatg
    10621 cgatgctgac caattcctcc gggacgtcgg cttcaaagcg ttggcgatag tacaggctca
    10681 gccgcggccg gctcgagagg atatcgaaca gcggcaggct ccacagcggc accgccagaa
    10741 gcgtataaag aagatccgcg ggatttgctt tcccgatttt ctcgctgaga cttcctacct
    10801 gcggcatctc aaagccagtc ggcgatagca ggataagcga gcgaatcaaa ccggggtttg
    10861 cctgggctgc cagcgctgca aattctgcgg aaaggccctg ggctatcaca tcagcaggtt
    10921 cgcccacttg ctcgcggatg aactcggtca gcgcggcctg gtacatactt gggcggtaag
    10981 gtcggtcgct gcgctctgag ccgccaaaac ccggcaaatc cagcgcgtaa accggacgct
    11041 gggagcggaa ggcgttgaag atcggggcga tgtcgtgcga gccggcggca gcgtggatgc
    11101 cgtgtaggat caccagcggt cgtccgctgc tgcgtgtatc ggcggtaaag tgcagttttc
    11161 cggcggtcgg agcctcaaag ctgccctgct ctgcctccaa cacgcctttt accctggcgt
    11221 gatggtcgat atacctgcgg ctgtagagta tccaggccgc tgaggttaat actccggctg
    11281 tcaccagtcc ggatatgcct cggggcagga atccgcgctt tttctttctt ttttctttca
    11341 tgaaacactc cagacgatta actgttaatt ccattataaa tgactttcgc ggacatccaa
    11401 aatccgcagt ccgtacattc tgtggaagta aggtcccccg attctggtag aatcaagctg
    11461 atgaaaacga aaacccgccg ctcgagggtc gatccgctgc gctggttgat tggtggattt
    11521 ggcctctttt ttttactttt cagcctgtac atactgctca aagattacac tgcctacaga
    11581 gcctcttccc ggctgtatcc aggcggcgta agcgtggcgc aaattccaat cggcgggctg
    11641 acgcgcgaac aagcggaaca acgcctgacg gaagcctttt ccgcgccagt ggaactgcgt
    11701 tacagcgggg caaggatgca gttcgcgccc gcggagctcg gcttctcgct caacgcggca
    11761 gcttcgctcg accaagtaga ccagcaaatc ccgcgcggcg cttggtggcg gcacctgtgg
    11821 ggccagagct caccggctta cacgcaggac ttcccgctcc aggccagcct ggacgcggcc
    11881 gcagcgcgcg ctttcctggt taacacagtc cagccgcgct acgaccaggc cgccagcgaa
    11941 gccctgccca tcctttacag caccaattac actctcgggc agtccggtta cgcgcttgac
    12001 ctgaacggcg cgctggccgc gctggaaccc gcgctctttt ccgcttctga ccgtacagtc
    12061 gacctgccca tccgggaaac gccggcgccc ggcctgaacc cccgcaacct ggaagtcttt
    12121 ctgcgccaga ccatccttct ggacggcttt gacgggctgg tggaaattta tctacagccg
    12181 ctcggcgggc aagaagcgct gcactttgct gttcgcgaca accagacggt cgcccccgac
    12241 gtcgcgtatt ccgctgccag caccatcaaa atcccgatta tgagctccgt cctgaaacat
    12301 ctggaggaac ccactcccga taccgcgcgc tactggatgg aacggatgat catccattcg
    12361 gagaatcccc cgagtgacgc gttgatgcgc acctacctgg acgaaaacct cggtccgctg
    12421 attgtgaccg aggatatgcg gacgctgggc tatcagaaca ccttcctggc cggttacttc
    12481 tacgccggcg ctccgctcct tcagcgcttc atcacccctg ccaattcccg tacggatgtg
    12541 tatctggatc cggatttcta caaccagact gtgccttccg agattggcga cttgctcgcg
    12601 cgcatatacc gctgcgcgca cgccgccagc gcccaggacg cgctctttga cgggcaggtg
    12661 agccaggcgg aatgccagac tatgctggac ctgctcggtc agaacaaaat cggggcgttg
    12721 attgaagccg ggctgccgcc gcaagcctcg gtcgcgcaca aacacggctg gacctctgac
    12781 ctggacgggc tgctccacac catgagcgac gccggcctcg tctccacccc gggtggggat
    12841 tacgtgctca tcatctttgt taattccgaa aaacagcttg tttttgacga aggcaactgg
    12901 ctttttgcac gcctttcaca agtcatttat aatgcctata atctgcagca gcaggcagcc
    12961 tggttgcctg gaaattaggc cgggttcata acccctgcgg cataggacgc tgaccatgag
    13021 agtcatcctg acccacgagc aagccgattt agatgcattg gcttcccttc tgggcgccca
    13081 tcttctggac aaggacgctt atgccctgct gccccgccag attaaccgca acggtcaggc
    13141 cttcctccaa aaattccaac aggaactggg ctttaacacg gtcagcgacc tcccgggcga
    13201 atcaatcacc caggtgacgc tggtggatac tcaatcgctg atcactctga aaggcttctc
    13261 ggcgcgtacg cacgtgagcg tgatagacca ccacccgcgc aaggcgcata ccgatcccga
    13321 atgggcagta gaactgcatg aaaccggcgc ttgcacgacc gttttggtcg aaaagctcca
    13381 gaaggcccaa accccgttct cagcctccca ggcgacccta ctattactgg gaatttatga
    13441 ggataccggt tcgctcagtt acgccatgac cacccccagg gacctgcttg cggcagcttt
    13501 cctgctggag cacggcgcgg atctgagcac ggcgtcgcgc tacctgaacc ccccactttc
    13561 caacgcgcag atgctcttat acgaccgtct gatgaaagac atcaccgcgc ataaaatcca
    13621 cgatttcacc atcctggtgg ctaaagccaa tgcgctggat atccaggacg aaatttcgac
    13681 cgtggcgcac catatgcgcg aattcctgaa cccggacgga ttggtgctct tggtttccac
    13741 ccgccagggc atccggctgg tggcgcgctc tacgaccgat gagattgaca tggcgcagtt
    13801 ggcgcaccgc tttgggggcg gcgggcacaa acgcgctgct tctgccttaa tccgctcaga
    13861 aagccgcccg acgccgaacg aagtgcccgt tttctggaag aaaacctacc agtatgtggt
    13921 cgagatgctt ccggaaattg tacatccctc catgcgcgta caacaaatca tgtcccgcaa
    13981 gccgctcttg gtctccccgg acatgccggt ggaaaccgtc gccgacctga tgaagcgcta
    14041 cggctttgaa ggttacccgg taatcgaaga gggtaaggtc gtcggtctga ttacccgccg
    14101 caatgtggac cgcgcgctga gccaccggat gatggccaac gccggcatcc tgatggacgc
    14161 gggcacggtt tacgttaccc cgggcgacac cctggattac ctccaggaag ttatggcctc
    14221 cagcggctgg gggcaggtgc ccgtgttgga cccggaaagc ggcgaaatca tagggattgt
    14281 gacgcgcacg gatttgataa acacgcacgc gctgcaaaac aatattccca accaggatga
    14341 aatggtgcgc cgcctgcaca atgctatccc ttatccgcgc caaatcctgc tgcaggcggt
    14401 cgcgcggctg gcgggcaaac tgaacatgca ggcttatatc gtcggcggtt ttgtgcgcga
    14461 cttactgctc aagcagccca gccaggattt tgatattgtc gtggaaggcg acgcgaatgt
    14521 gttcgtggcc aaactggtgg aaatctacgg cggcagagcc gtcacgcatt cgcgctttgg
    14581 cacagccaag tggataatcg gcgacgagcg cgaagccctg gcgcagaaac tgctgccgga
    14641 ccaaccgatg gactttcagg ctctgcccca gcatctggac ttaatcaccg cgcggacgga
    14701 gttctacgag cgcccggctg ccctgcccac cgtcgagcgc agtggcatca agatggactt
    14761 acaccgccgc gatttcacca tcaacaccat ggcgctgcgc ctggacgcgc cttactacgg
    14821 caaactgtac gatttctggg gcggctatca cgaccttcag aaccatatta tccgggtgct
    14881 gcacgcgctt tccttcgtgg atgacgccac gcgcatcctg cgggctatcc gtttctccac
    14941 caggtttgat ttcaggatgg accccagaac gctcagcctg ctgagctcca gcctgtccct
    15001 cctgaatgag atttcaggat caaggctgcg gcatgaattg gatttgattc tgctcgagtt
    15061 caacgtcggc actgccctga gagcaatgga ccgcctgagc gtcctcgagg taatcaaccc
    15121 gaacctgaac tggaaccccg ccctggaggc acgcatcgcg tttctgaatc agccggccgc
    15181 ccaagccgaa tggaaaacag aaacccacga gaaccagttg aacttgcggc aggtgttggg
    15241 ctattgctac tggctggaag acctttcacc ggagcagctc gagcaggtta gcgcgcggct
    15301 gcgcatcccc gcccgggtta ccagcgccat cagcgatacg gcgaaactgc ggaggctgct
    15361 gccggagctt ggcgccctga agccaagcca ggtcgtgaac gcgctcaagg gcatctcccc
    15421 cctggcgctg accgcggtct atcagagcac agaagaccct gccctgcgcg agccgctgcg
    15481 cctgtatcag acgcgctacc tggatgtcaa accgctgcac agcggcgacg acctgcgcgc
    15541 gcgagggctt ccgccttccc ctgaatttca gagaatcctg aacgctctcc gggatgcctg
    15601 gctggacggc agtgtgtcct cccccgagga agaagacgcg ctgctggaac aactcctcaa
    15661 cgcaacatga gttcccaccc cgcagtggca agcactttcc agcttccgga cggtgaagaa
    15721 attctcatcc gcccaatccg tgaagctgac ctcccggcgc tggaatggga aggcgaatac
    15781 cggcacttca gaaagctcta cgctgaacat ttcgaagcct tccgggcggg caccgcgctg
    15841 atttacgttg ccgagactcg cgctggcgtg atgatcgggc agatatttat ccagctctac
    15901 tcccgcaaca aagaagccgc cgacgggata cacagggcgt atttgttttc tttccggatt
    15961 aagccctccc accgcagcat gggtctgggc acttatatgc tcaggtttgt ggaagaccaa
    16021 ctgcttatgc gcggctacac cacaatacgc ctgaatgtgg cacaggacaa cacccgggcg
    16081 cgcgcgttgt acgaacgctg cggctatcag gtgattggct acagctcggg cgtatggcag
    16141 tatcaggatg accaaggcga gtggcagacc atgcacgaac ccgcctggaa aatgtccaaa
    16201 cgtttgcgct gatgcgcgag gttttcgcga gcaatacgcc gctctacggg cataatccgg
    16261 tattcttaaa agaaagaccg gccgcaatgc ggccggtctg aatttcaaac gaaggatgtc
    16321 actcttcctg ttctggcagg tcttcttcct cagctggctc agctgccgac tcttcctccg
    16381 cgggcttgga ggccttgttt tcatcgatcc agcgcagcat ggtggcgcgc atttcggcat
    16441 aatccgcgtc cgggaacagt ttggtaatcg cgaacagccg tcttccgcgc gtggtcgaga
    16501 cgtaggttcc attcttgacg tcttcaatcc cagtaatatc cgtcttcgag agcttcaggt
    16561 ccggatattt cggctggtgc agcaccaacc cgtcttccgt aaccgtaaat gtatattctt
    16621 cccacaggga tttgcgctgc tgatacgccc gccagccgga atagacgaac agggcggcta
    16681 tcaggggaat catccagacc agagacttca tgctttcgcg gttccagttg atacccacgg
    16741 agagcgcggc cattaccagg aaggtgatgc cgtataccag gatgagcgtc ttcttttggg
    16801 cgggtaattt tgccgggtcg agtttataag ttttcatcga ttctccttaa gtctttaccg
    16861 tttgattata ctctctatca tggaagaatc attcccggca attcgggcag gttcactgga
    16921 aaaggttttc aaggcgatca ggggattgat aaaggatcaa gaaagatttt cccaactctt
    16981 cgtagattgc caaagagacg aaaatattat ggcgaacaag cctacgaaca gcctttcgcc
    17041 tcttacgccc ctaccccagc gaactcaatt tatccattga tcctgatcag cgatctctgg
    17101 gacggggggt tgagctttga tagaaagagg cgtattctat caaacgttgt tcccttcccc
    17161 agcggattga ttgcgccaca aaaacacttg ccggagggga ggggtcaggg ttaggatacc
    17221 tgtggtgcat tatggagaat gaggctgcaa aaccagatca tcctgagagt caagtttttt
    17281 tcaatatcct cctgctattt tcatttccag tctgcttgtt ttttgatata attaattctt
    17341 agcatattta tcgataccaa tgattctaat ccgcccggtg ctgcagcgcc gcgcaacaaa
    17401 taaggaaggt cacatcaatg aaagaatttt cgacagcctc tgtacgcaat atcgccctcg
    17461 cgtcacacag caactccggc aagaccatgc tcaccgaagc gttcctgcac ttcaccggtg
    17521 ccaccacccg cctcggcaaa atcgaagacg gaacaaccgt ttcggacttt gacgacgaag
    17581 aacaccgccg cgggatttcc ctgttcacct ccgtaattcc ggttctctac aaagacctga
    17641 aattcaactt tttggacacc cccggatatt cggacttcgt cggagaagta gtctcggcta
    17701 tgcgcgtggc agacagcgtc ctgatcctgg tcgattcggt tggcggcatg gaagtgggca
    17761 cggaaaccgc gatgaactac gcggccaaat ttaaactgcc catgtttatg gtaattaaca
    17821 agatggaccg cgaaaacgcc agttttcaaa aagcgctcgc ttccgtccag gaacagaccg
    17881 actaccgtct gatccccatg cagctcccct ggggcgaaaa agcggatttc aagggcgtga
    17941 ttgacctcat cagcatgaag gctttccagg gcgagggcaa aaccgccgtg gaaatcccgg
    18001 ctgaattcct gccggaagca gaagaagccc gcctcaaact gattgaagcc gccgccgaag
    18061 gcgaggattc actgctggaa aaatactttg aaaatggcga cctgacgacg gaagaagcgc
    18121 tgcaaggcct gcacagagcc gttcagatta gcaacgttat ccccgtgatg gtggctgccg
    18181 gcggtcatat ggtcggcgtc gcgccgctgc tggaagcgct gagcacgctt gccccgtccc
    18241 cgctcgagca cgccgacgtt gtcgcgaaat cccccaaggg cgacgtgcag ctgaccccgt
    18301 cggattccgg tccgcttgcc gcttatgtct ggaaaaccac ggccgacccc tttgtgggaa
    18361 agcaaacctt cttccgcgta ttttccggcg tgatgagctc ggatacccgc gtctggaacg
    18421 cgaataaagg cgaggaagaa cgcctggctg gcatgcaggt gccttttggc aaggaaaacc
    18481 tgccagtctc cgtcattcac agtggcgata ttggcacggt tgccaagttg agcctgacca
    18541 ccaccggaga tacgctcacc accaaggaac agagcctgat tcttcccatg cctgaatacc
    18601 ccagcccgct ttaccgcgta gctgtcagcc ccaagaccca atccgacgcc gccaagatga
    18661 gcaccacgct ttcccgcttg tgcgaagaag acctgaccct cacctggcac atggaacccg
    18721 ccacccgcca gacgctgctg ttcgggatgg gcgaccagca cattgacgta gcagtgcgcc
    18781 gcgccgagca gaagttccag atgagcatga acctgctgga acccaaggtg ccgtatgaag
    18841 agcacatcac caaggaagcc agcgctatgt accgccataa gaagcaaacc ggcggctcag
    18901 gccagtttgg cgaggtccat ctgaaagtct ttccgattaa ggatgaggat ttcagcttca
    18961 gcaatgacgt ttttggcggc gcgatttcca acagctacat ggccccaatc gagaagggta
    19021 tccgcgccgt gatgaaagac ggcgttgtgg caggctaccc ggtgcacaat gtcgccgttt
    19081 ccgtttttga cggcaaggaa cacccggtgg actctaaacc aattgcgttc gaaattgccg
    19141 ggcgcgaagc gttcaagcta gctttcaaag atgccaatcc ggtgctctat gaaccgatta
    19201 tgaaagttac ggtggtcgtc ccagaggcga acatgggcga cgttttgggg gatatgaaca
    19261 cccgccgcgc gcgcgtgcag ggcatggaca ccgaaaaagg ccgttctgtt gtgaccgtca
    19321 cagtgcccct ggctgaaatg ctgcgctata ccaccacctt gcgctccatg accggcggac
    19381 gcgggtactt cagcatggaa ttcgaccact acgatctggt tccgagccac ctggcgcagg
    19441 agatcatcga tgcccgtcag aaggaactta aggaagagga ataatcccct tcagtctggt
    19501 ttcacaggcg gccgccattc ctggcggccg cttttttgtt ttcctgccgg cggatttacg
    19561 aggaatgttg agtgaccctg gtctgctagt taatgcgaac acagcaaacc gggaaaaacg
    19621 gtgtggcaat accctgctgc cccgaagtat gcctgtttgc cggcgctaag cgttagggag
    19681 accgtttcgc caagatcctg tcattctgaa cgaagtgaag aatctctcgt gggaataagc
    19741 cagatccttc gctcgcgctc agtatggcaa gccgttaaag ccgggtccag a
//
LOCUS       DAOQAD010000028        19195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_2986_length_19195_cov_5.356322, whole genome shotgun sequence.
ACCESSION   DAOQAD010000028 DAOQAD010000000
VERSION     DAOQAD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19195
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2986_length_19195_cov_5.356322"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKL60_03780"
     CDS             complement(<1..414)
                     /locus_tag="PKL60_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNW95289.1"
                     /translation="MVNNEVLIKSKKRVQQHGEVFTPKAIVDAMVTLPGLDEVIIQAT
                     TTVLEPAAGEGAFLINILERRLYLLAEQFSDDLARFENYALLAIYPLYGLELLEDNVK
                     KCALNLFITFHDFYKDFAAKLERKPKSNVEESAKTI"
     gene            complement(586..963)
                     /locus_tag="PKL60_03785"
     CDS             complement(586..963)
                     /locus_tag="PKL60_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95290.1"
                     /translation="MTTNDERIKIMSMLQEGKIDSSEAARLLQALDESAKPAAEEAKA
                     AKKPRFLHIKVTRLMDGKSIADIRVPISIVNAGMRMGAKFSPQLAGMDPDMLSELLKS
                     DEPAQIIDVSDEEDGEHVQIFLE"
     gene            complement(982..1362)
                     /locus_tag="PKL60_03790"
     CDS             complement(982..1362)
                     /locus_tag="PKL60_03790"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNW95291.1"
                     /translation="MPNLPDTCPICHSGLVVTRAYCPACETTLEGYFQFEADPFTVLT
                     REQREFLLTFVRCEGRLNRMEEILGSSYPTLKNRLLEIIHALGFETEKSPAAPAQSRA
                     QILENLENGSITAEEALEALQALK"
     gene            1596..3149
                     /gene="ftcD"
                     /locus_tag="PKL60_03795"
     CDS             1596..3149
                     /gene="ftcD"
                     /locus_tag="PKL60_03795"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNW95292.1"
                     /translation="MSVKIVECIPNFSEARRPEVVDQIIAAVQAVPGVGVLDRHSDLD
                     HNRTVLTFVGEPAAVEEAAFAAIAKAAELIDLNQHEGQHPRIGAADVVPFVPISGMTM
                     VECVEMARRLGQRVADKLGIPVYFYEEAALKPERRNLEDIRRGEYEGLKQAVLEDPTR
                     APDLGPARLGSAGATVIGAREALIAYNIYLNTPDVSIADKIARRVRNSSGGFRYVKAM
                     GVLVGGLAQVSMNLTNFRRSPIAQITETVRREAARYGVGLHHSEIVGLVPNQALVNAA
                     RWYLQLDGFENEQVLDSRLFSWQSGQVSAAKPEQADFLDLVAEGTPSPGGGSAAAHSG
                     ALAAALVAMVARLTVGKPRYAEVEAEAWQVIARAEELRKALTAAITLDAQAFDGILQA
                     RKLPKADEAQQQARTAAIYSATLHAARVPLETAGDCLSVLRLAARMAEIGNLNAISDA
                     GAGAQLAQAAFRSAALNVRVNLLGLEADPEPARLLGELTALEGQADEASQAVDQALAK
                     RAGLRAKTA"
     gene            3203..3757
                     /gene="dcd"
                     /locus_tag="PKL60_03800"
     CDS             3203..3757
                     /gene="dcd"
                     /locus_tag="PKL60_03800"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNW95293.1"
                     /translation="MGLKPDHWIKRMALEQRMIEPFEEAQVRDGVISFGTSSYGYDIR
                     VADEYKIFTNIYSAVVDPKRFDPRSMVDFTGEVCVIPPNSFALARTVEYFRIPANVLT
                     ICLGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPAKIYSNEGIAQVLFFEGDE
                     PCEVTYAQKKGKYQGQQTIVLPKL"
     gene            3770..4348
                     /gene="yjjX"
                     /locus_tag="PKL60_03805"
     CDS             3770..4348
                     /gene="yjjX"
                     /locus_tag="PKL60_03805"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003459.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HNW95294.1"
                     /translation="MRRSVALGSTNPVKLEAVKEGFRTIFPADELTFAGVEAASGVSA
                     QPMGDAETRQGAANRALGACQALPDADFWVGVEGGAETLPGAPEIMFTFAWVVILSRE
                     RRGQARTAAFQLPEAVAELVRGGLELGDADDLLFQTSNSKRRNGAVGLLTGDVITRSE
                     LYTPAVQLALIPFLRPELYPAETPGMERDNEP"
     gene            4338..4784
                     /locus_tag="PKL60_03810"
     CDS             4338..4784
                     /locus_tag="PKL60_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HNW95295.1"
                     /translation="MSLEAEYWKKKDLRDMNPEEWEALCDGCGKCCLFKFTNPDEETV
                     RFTNVVCRYMDLETCRCTDYDNRHVNVPDCIYLTPKIAKTVNWLPSTCAYRLVARGED
                     LPWWHPLKSGSPQSVFLAGEAVRGKVVSEEDVNPDDMEDMVVDWFR"
     gene            4850..5185
                     /locus_tag="PKL60_03815"
     CDS             4850..5185
                     /locus_tag="PKL60_03815"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNW95296.1"
                     /translation="MDTEAMKLLIAVIQTTDSDLLSEVLQKHKILFTRLPSVGSFLQE
                     RNVTFLVSCKQADEDSVRELLISTCSKRIAFIATPIENAPLPMPYPTETVVGGIHLFS
                     LDLEHFEEF"
     gene            5188..5454
                     /locus_tag="PKL60_03820"
     CDS             5188..5454
                     /locus_tag="PKL60_03820"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNW95297.1"
                     /translation="MKLIIAIIHDEDNDRVSTTLNAEGIRATFIASTGGFLRSGRSTL
                     LVGLEEEKVPRALQILRENCTTGKEGKEKKAIVFVLNVQDYIQV"
     gene            5520..6149
                     /locus_tag="PKL60_03825"
     CDS             5520..6149
                     /locus_tag="PKL60_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNW95298.1"
                     /translation="MNFGKAFSYVFDDQRWLEKIIIPILYSLIPVVGWMVMMGYVMRT
                     IRNVAEGVEFPLPVCDFGDDLALGFKMFVVNLAYAVIPLLLMLLLMPLTYAVESSNSM
                     PVLAILGLIILSLFLFVYGIVYSLIFPIIQANVAIKNSISAGFEFKQIFGMLTKNIGA
                     WLLVYAGLLIGGLIAPLGSIAFFIGVFLTSMYSQLMTAHLAGQAYALTK"
     gene            complement(6192..6773)
                     /gene="amrA"
                     /locus_tag="PKL60_03830"
     CDS             complement(6192..6773)
                     /gene="amrA"
                     /locus_tag="PKL60_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNW95299.1"
                     /translation="MPQKLTPDEWDALMRIAKEAVLAAANGRRPAALPSADLPPRLLE
                     DGAVFVTLTKSGQLRGCIGSLEAWQPLAEDVRLRAYQAAVEDPRFPPLEPAELPRIEI
                     EISRLTEPVALPYEHPEDLPRLLKPHQDGVILADGRRRATFLPQVWEQLPRPEEFLSH
                     LCAKMGARPSLWREKVLDVWIYHVEEHKEKPEW"
     gene            6887..7765
                     /locus_tag="PKL60_03835"
     CDS             6887..7765
                     /locus_tag="PKL60_03835"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNW95300.1"
                     /translation="MSKSMHEIDDKPGFQLPFRKHEQVSTPTPAPAPKTAGPKIKTSA
                     TWQALRVVLGGALIVATLFTLWTPSSLLAESLQERMARALAASGNASLTQTAAAGMPA
                     GFPSNKIGIVVGHRGNDSGAVCPNGLTEVEVNSNVAAFVQQKLSKMGYEVELLDEFDA
                     RLAGYQAALLISIHSDSCEYFNDNATGFKVAAALSTSKGDNATRLVSCLADRYQKETG
                     LQYHYQSITEDMTSYHAFSEIDPLTTAAIIEVGFMNLDQDILTRKPEKLADGIIAGLR
                     CYLENEGVEPTPTPKP"
     gene            7768..8811
                     /locus_tag="PKL60_03840"
     CDS             7768..8811
                     /locus_tag="PKL60_03840"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNW95301.1"
                     /translation="METNSPNPESLIAQAKQAVLRGDRAAAKALAEQALALNPRSVDA
                     LLILAGVSDAQTSVAYLKSALDLDPGNQIARLGMLWAARELRKTHAAGWTAEKPQAPQ
                     PKLTPLANFRRRKPGLWIALLLALLCASVYALYAVGSFSPRAVSAGRQFTLYESTSLV
                     KPSLTPTITPTPTQTPTPTPTLTRTPTPTSTSTPTPTLEPTPTEVPEVIITYVPQEEE
                     PTEVYVPPEETQGTRWIDVDLGDQALYAYEDDTLVASFLVSTGLPNTPTPTGRFYVYV
                     KLLFDDMQGPGYYLPDVPYTMYFYEGYGIHGTYWHSNFGNPMSHGCINMETGDAGWLF
                     DWSYVGILVNIHN"
     gene            8811..9842
                     /locus_tag="PKL60_03845"
     CDS             8811..9842
                     /locus_tag="PKL60_03845"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNW95302.1"
                     /translation="MDFFTQKFTRREFLKLMGLGAVAAALPNRLVKAALTDFTEGTWA
                     RITRFRLYVFAEPSEKSKVQDLLKFNQLVRIEDTLTVKDSKGNEHPWLKLGEDAYIQP
                     VFVQYVETHRNVSDAPIPEGGCLGEITVPMIDAYYTPRGTYSKRHYYYSSTHWVRSRV
                     FDEFGVPWYELPDDMNGASYYVRAYAVRLVPAEELTPLSPEVPPEQKRIKLDLKSETV
                     TAYEYDKPVFSTLVSTGLAEGSTPYGTFKTHRKRPYRRMVLQSGDLNVDYDLPGVPWV
                     SYITMSGVAFHGAYWHSNWGHRMSNGCINMRSPDAKWLYRWCNPTVPADVEYLVGETG
                     TRVDVVYEM"
     gene            9922..10341
                     /locus_tag="PKL60_03850"
     CDS             9922..10341
                     /locus_tag="PKL60_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95303.1"
                     /translation="MNKAVFEGLVFDPSGSPLSVDYVGSEAVYILNDDGFLRHIPAAD
                     VDRQVWEALTAPLVGNEDAISAQAAKMLGQEDIFSIAVIRSQLENKDKQFEAVQAAGL
                     PEDARAYLGMVGFRVTVDLHGDVIDIHQPGIVEEDGD"
     gene            10381..11211
                     /locus_tag="PKL60_03855"
     CDS             10381..11211
                     /locus_tag="PKL60_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW95304.1"
                     /translation="MTDDYIIDVSDESFELEVVNYSRQIPVVMDFWAPWCIPCRVQSP
                     LLARLAQEAEGAFRLARVNVDEQTKLAERLKVKNIPAVKAFVDGRVVSEFTGVLSEPN
                     LRQFLQNLLPDSGDLLLEKGKSLILLGDYAEAEKALGRFVSGNPRHPGGILALARLML
                     IEGKAREAKILLANFPASHEFSTAQQLKPVVQAFLWLDTAREAPQDALEAAYRNSLKL
                     ARSGKILLALDGFLDILRKDKDYRDDEVREVYLGLLEVLGEEHPEVRQYRADLTNVLF
                     "
     gene            complement(11388..13250)
                     /locus_tag="PKL60_03860"
     CDS             complement(11388..13250)
                     /locus_tag="PKL60_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW95305.1"
                     /translation="MVYDVIVIGAGLSGLSAASLLAKRGLQVLVIDKSYNPGGSCGIF
                     KRDGVIFDQGSAMLYGFGEHGYNSHRFLFNCLEEPIDMIRHDLLYVVNFKGQRIRFFA
                     DVEQFIVELSGVFPKEAANIRRFYHDMLRMYQHVMVENPSYTSADESDPQESLPALRK
                     HPISFLRFFSYLNKGADALLGKYFRDPEIFKFFDKLTSTYCYATVKEAPAVLASVMFV
                     DNHVGGSYYPAGSTLFLPGKLEKVIEENGGDMLPEAEVARIMFQDGKPSGVELTDGRQ
                     LFARDIIYSGTVWNLYGKLLDPAETTAARRQWAASQAPTYPSVVLYALVDKTVIPEDT
                     APVEMLVGNPDLLDESEVTAYIFSIDDHTLCPPQYHTIAAIGPSFETWQGLSPEEYQQ
                     KKAREVERLTQVLERRFPGFRAGLCFSELATPRTIERYCLKNGGAVAGPKQMLGQHML
                     KRLHTRTEWENLFCCGESTVQGTGTPSVTTSGISAANAVLKKRGLTPFKFRPGMPNYV
                     RIVDKPFTPERLFADYPMEQQAVMRAAFRCQFCENPGCSAGNALDFRGMLRRVSVGNF
                     IGARKIAAGIDAKTGASQEALAQAEQNCVVNTAHGEPVAIRSVMAYLLNQTDSA"
     gene            complement(13290..14321)
                     /locus_tag="PKL60_03865"
     CDS             complement(13290..14321)
                     /locus_tag="PKL60_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95306.1"
                     /translation="MESKPKLKFHNFFALVTYLIMVAVNALANILPINGKLTGEISDS
                     RPNLFAPAGETFIIWGVIYLLLLLYTLYQMGLLRAKNEANNTKLLAKVNIFFGISSLA
                     NTVWIFLWHYERIVWSNIPMLVILLCLIIIALMLKKGASTRREKLLVRAPFSVYFGWI
                     TVATIANVTTTLVEIGWDGLGLQQSFWTVVILLVGTLIAGLTTILNRDVAYGLVPIWA
                     YLGILYKHLLPEPAGFAAQHLAVIIAVTACLVLLVVALFMAIFGKKKEAPEPQPTAMP
                     EPIPTPAAEPLPMPGPIAPPEPAPEVEPAPEPEAGAAVESPSPTDGINPPEMSEDTKP
                     LTPPQDAQL"
     gene            14620..15156
                     /locus_tag="PKL60_03870"
     CDS             14620..15156
                     /locus_tag="PKL60_03870"
                     /inference="COORDINATES: protein motif:HMM:NF037099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95307.1"
                     /translation="MEDKYKGLYRFHLVIGFLHLIQGVLLIVPGNENPYPMVAIFLRP
                     TPETRKLVPDPRLFRELPFGAAAAIFRLISDAGHSQSAANAAPALLGARGIPSAFIPA
                     SSAHGPVGGYPGSSDNGASGFRGLLDFRGRLECMRLERTRQKKTERPAQGGARENLQK
                     PKVQSVFRVSGLLTLGTR"
     gene            complement(15253..15453)
                     /locus_tag="PKL60_03875"
     CDS             complement(15253..15453)
                     /locus_tag="PKL60_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95308.1"
                     /translation="MDNPDQETLTQALRSINSLLDKCEKVQGKFQAGTSQHTLLKNRI
                     SALRIASALIFRELGNLSQTEE"
     gene            complement(15572..16141)
                     /locus_tag="PKL60_03880"
     CDS             complement(15572..16141)
                     /locus_tag="PKL60_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNW95309.1"
                     /translation="MNILLINGSPHKDGNTFKSLKVIEAVLEKHQAATTIFQLGTEKL
                     RGCNGCDSCAATNRCVYQDDLCNSLIEAIINADGIIVGSPVYFAGPNGALCALLDRVF
                     YAATNQANIFAHKPAAAVVNCYRAGATAALDRLNKYFTISEMLVVSSNYWNMIVDADT
                     DRVEKDAYGREVLERLATNMVWTIGKIQE"
     gene            complement(16166..17404)
                     /locus_tag="PKL60_03885"
     CDS             complement(16166..17404)
                     /locus_tag="PKL60_03885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HNW95310.1"
                     /translation="MNETVLIEKCVYGGDGMGRLADGRAIFVPFTLPGEEVLVEIWED
                     NQNFARGRAVEVRGPSAERVAPPCPHYGACGGCHYQHLPYARQLELKLQILADQLARL
                     GGIPNPPLSGIMPSPECWHYRNQVQFHLDALGQLGYMDLTGKQVVPISQCLLPLADLE
                     TLRAQLDLSPEAGLSRVVLRQDSQGEQFIVLEGPDELAPEVSLDLPASVSYLGPDRQA
                     ITLAGQDQLVYEVLGKQLSVSPESFFQVNLAVAEQMLSHVLSLLPARNDLRVLELFSG
                     AGFFSAFLAEQCRELIAIESSLSACFDFANNLDAYDHVSLYEGAVEQVLPGLTADLKG
                     VDLVVLDPPRAGLRPAARKALQELGAGQIIYISCDPSTLARDLKHLCAAGYSLRDVRA
                     FDMFPQTYHIETAAVLSRAK"
     gene            17695..18789
                     /gene="serC"
                     /locus_tag="PKL60_03890"
     CDS             17695..18789
                     /gene="serC"
                     /locus_tag="PKL60_03890"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNW95311.1"
                     /translation="MKYGRVYNFSAGPSMLPEAVLEEVQIELLNYQGSGMSVMEMSHR
                     SKVFEDILHEAEKDLRELMNIPDNYEVLFVQGGGTVQFAMVPMNLMKNGAADYVNTGA
                     WSKKAISEAKKFGKVNVIASSEDRNFSYIPDMRNVSITPDADYVYICENETINGTTWQ
                     TLPDTQGKPLVSDQSSMFLSRPCDVSKYGLIWAGVQKNVGPAGLAIVIIREDLIRRED
                     LDPKVPTYLRYDTHADNDSLYNTPNCWAIYVCGKVFKYLKKLGGLEAMNKLNQEKAAP
                     LYAYLDRSEMFHGTVEKKDRSLMNVPFSTGDKELDAAVVKASLEAGFDNLKGHKSVGG
                     LRASIYNAMPKEGVDALVEFLQKFEEEHLK"
     gene            19001..19074
                     /locus_tag="PKL60_03895"
     tRNA            19001..19074
                     /locus_tag="PKL60_03895"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19035..19037,aa:Val,seq:gac)
BASE COUNT         4162 a         5333 c         5605 g         4095 t
ORIGIN      
        1 aatcgttttt gcgctctctt ccacgttgct ttttggtttt cgctcaagct tagctgcgaa
       61 atctttgtaa aaatcatgga aagtaatgaa aagatttaag gcgcatttct taacattgtc
      121 ttctaacagt tcgagtccat acagagggta tatggcgaga agcgcgtaat tttcgaaccg
      181 ggcaagatcg tcggaaaatt gctcagcaag caaataaagc ctgcgttcca gtatattaat
      241 cagaaaagca ccttcgcccg cagccggttc gagtacggtt gtcgtggctt gaataattac
      301 ctcatccaaa cccggcaggg ttaccatcgc gtccacgatt gctttgggag tgaaaacctc
      361 tccatgttgc tggacgcgct tcttactctt aatcaatact tcgttattaa ccaatattcc
      421 gacctttgta ctggttcgta aaaacagtat taatttccaa cctgatgctg tctacagtat
      481 ctcaattata catgtcattg gatttgcccg ccctaagcac tgcaaatttc accctagttc
      541 agatttaata cgagcgtcct tcagagacgg atgaatggga gagctttact ccaagaaaat
      601 ctgcacgtgc tcgccgtctt cttcatcgct gacgtcgata atctgcgcag gctcgtctga
      661 tttcagcagc tcggacagca tgtccgggtc catcccggcc agctgcgggc tgaacttcgc
      721 gcccatgcgc atgccggcgt ttacgatgga aatgggcacg cggatatccg cgatggattt
      781 gccgtccatg agccgggtca cttttatatg caaaaagcgc ggctttttgg cagctttggc
      841 ttcttccgcg gctggcttgg cgctttcatc cagggcttgc agcaggcgag ctgcctccga
      901 ggagtcgatt ttgccttcct ggagcatgga cataattttg atgcgttcgt cgttggtggt
      961 catgagaacc tcagtttgtt tctatttgag cgcttgcagg gcctcgaggg cttcctccgc
     1021 ggtgattgag ccgttttcga ggttttctaa aatctgtgcg cggctctgcg ccggcgcggc
     1081 cgggcttttt tcggtttcaa aaccaagcgc gtggattatt tcaagcaggc ggtttttcag
     1141 cgtggggtaa gagctcccca ggatttcctc catgcggttc aggcgccctt cacagcgcac
     1201 gaaggtcagc aggaattcgc gctgctcacg ggtgaggacg gtaaaagggt ccgcttcaaa
     1261 ctgaaaatac ccttccagcg tggtctcgca agccgggcag taagcccggg tgaccaccaa
     1321 tccggaatgg caaatggggc aggtgtctgg taaattgggc atggttttcc tctatggctg
     1381 cgcttgttta tgagcctatt ctaatgccga atttggaaaa tgcaagtagg aataaagaaa
     1441 cttaagcaaa ttatcaaagc atattatgtt tcttaaagac ttaatgaata atatcgaaac
     1501 atgcccttta ttcttgttct gcggcgtgcg gaaaaatcgg acggccggaa acgcgcggct
     1561 tggtataatc attgcagcaa aacctggagg caaacatgtc cgtgaaaatc gttgagtgca
     1621 tcccgaactt ttcggaagcc cgccgacctg aggtcgtaga ccagattatc gcggcggtgc
     1681 aggctgtgcc cggcgtaggc gtcctggacc ggcactcgga cctggaccac aaccgcacag
     1741 tgctcacttt cgtgggggag cccgcggccg tggaggaggc agcttttgcc gcgattgcca
     1801 aagccgccga gctgattgac ctgaaccagc atgagggcca gcacccgcgc attggcgccg
     1861 cggacgtggt gccgtttgtg ccgatttccg ggatgaccat ggtggaatgc gtggagatgg
     1921 cgcgccggct ggggcagcgc gtggcggaca agctgggcat tccggtctac ttttacgagg
     1981 aagctgccct aaagcctgag cgccgcaacc tggaggatat ccgccggggt gaatatgaag
     2041 gcctgaaaca ggccgtattg gaagacccga cgcgcgcgcc cgacctggga cccgcgcggt
     2101 tgggcagcgc cggcgcgacg gttattggcg cgcgcgaagc gctgattgcc tataacatct
     2161 atctgaacac ccccgacgta agcatcgccg acaaaatcgc gcggcgggtg cgcaactcct
     2221 cgggcggttt tcgctacgtc aaggccatgg gcgtattggt cggcggtctg gcgcaggttt
     2281 cgatgaacct gaccaacttc aggcgttcac ctatcgcgca aatcaccgaa accgtcagac
     2341 gggaagctgc ccgctacggc gtcggcctcc accattcgga aatcgtcgga ttggtgccca
     2401 atcaagcgct ggttaacgcg gcgcgttggt acctgcagtt ggacgggttt gaaaacgaac
     2461 aggtgctgga cagccggctg ttctcctggc agagcgggca ggtgagcgcc gccaagcccg
     2521 agcaagcgga ttttctggac ctggtagcag aaggcacgcc ctcgcccggc ggcggttcag
     2581 cggcggcgca ttccggcgcg ctggccgcgg cgctggtggc catggttgct cggctcacgg
     2641 tcggcaagcc caggtatgcg gaggtggaag ccgaagcctg gcaggtcatc gcccgggcgg
     2701 aggagctgcg caaggcgctg accgcggcaa taaccctgga cgcgcaagct tttgacggca
     2761 ttctgcaggc gcgcaaactg cccaaagcgg acgaagccca acagcaggcg cggactgccg
     2821 ccatctacag cgcgaccctg cacgccgcgc gcgtgccgct ggaaaccgcg ggcgattgcc
     2881 tttcggttct gcgcctggcg gcgcgcatgg cggaaattgg taacctgaac gccatttccg
     2941 acgcgggcgc cggcgcgcag ctggcgcagg cggctttccg ctcggccgcg ttgaacgtgc
     3001 gcgtcaacct tctgggtctg gaagccgacc cggagcccgc gcgactgctg ggcgaactga
     3061 ccgcgctgga ggggcaggcg gatgaggcct ctcaggctgt ggaccaagcg ctggctaagc
     3121 gcgctggatt acgcgccaaa accgcttaga aacgccagca ttggctggtc tgaggagaaa
     3181 cttgagaata gtggagtaag ggatgggatt aaaaccggat cactggatta agagaatggc
     3241 gcttgagcag cgcatgattg aaccctttga ggaagctcag gtgcgggatg gggtaatctc
     3301 ctttggaacc tcttcctacg gctatgatat tcgcgtagcc gacgagtata aaatcttcac
     3361 caacatctat tcagcggtgg tggacccgaa acgatttgac ccgcgttcga tggtcgactt
     3421 tacgggcgag gtgtgcgtca ttccgcccaa ctcgttcgcg ttagcccgca cggtcgaata
     3481 cttccgcatc ccggcgaatg tgctgaccat ctgcctgggg aagagcacct acgcgcgctg
     3541 cggcattatc gtgaacgtga ccccgtttga accggaatgg gaaggctacg ttaccctgga
     3601 aatttccaat accaccccgc tgcccgccaa aatctactcc aacgaaggca ttgcccaggt
     3661 gcttttcttt gagggggacg agccttgcga ggttacatac gcgcagaaaa aaggcaaata
     3721 ccagggtcag cagactatcg tgctgcccaa gctgtaggcg ggtgagacct tgagaagaag
     3781 cgtcgcgctg ggttccacca acccggtcaa gctcgaagcg gttaaagagg gctttcgcac
     3841 tattttcccc gcggacgagc tgacgtttgc gggcgtggag gctgcttccg gggtctctgc
     3901 ccaacccatg ggcgacgcgg aaacgcgcca gggggcggca aaccgcgcgt tgggagcctg
     3961 ccaggcactg ccggacgcgg atttttgggt tggcgtggaa ggcggggctg aaaccctgcc
     4021 tggcgcgccc gaaatcatgt tcacctttgc ctgggtggta atcctctccc gtgaaaggcg
     4081 cggtcaggcg cgcactgccg cttttcaact gccggaagcg gtagcggagc tggtgcgcgg
     4141 cggcctggag ctgggggacg cggatgactt gctgttccag accagcaact ccaaacgccg
     4201 caacggcgcg gtggggctgc taaccgggga tgtcatcacc cgctcggaac tgtacacccc
     4261 ggcggtgcag ctggcgctca tcccatttct tcgcccggaa ctctacccgg cagaaacgcc
     4321 tggaatggaa agagacaatg agccttgaag cggaatactg gaagaaaaaa gacctgaggg
     4381 atatgaaccc ggaagaatgg gaggccctct gcgacggctg cggcaagtgc tgcctgttca
     4441 agttcacgaa cccggatgag gaaaccgttc gttttaccaa cgtggtgtgc cgttatatgg
     4501 acctggagac ctgccgctgc acggattacg acaacaggca tgtcaacgtc ccggactgca
     4561 tctatctgac tccaaaaatc gcgaaaacgg taaactggct gccatccacc tgcgcctacc
     4621 gcctggttgc ccgcggagag gacctgccgt ggtggcaccc cttgaaaagc ggcagtccgc
     4681 aaagcgtctt cctggcaggc gaggccgtac gcggcaaagt ggtcagcgaa gaagacgtga
     4741 accctgacga catggaagat atggtcgtgg actggttcag gtgagacaag ctttttttcg
     4801 gaagcagaat ggagtacaat aaagttaacc aggcaaaagg aaggatgtga tggatactga
     4861 agcgatgaaa ctcctcatag cggttatcca gactaccgac agcgaccttc tgagtgaggt
     4921 gctgcagaaa cacaagattc ttttcacccg gcttccgagc gtcggcagct tcttgcagga
     4981 gcggaatgtc acatttctgg tcagctgcaa acaggcggac gaggacagcg tgcgggaact
     5041 gttgatttca acctgcagca aacgcattgc cttcatcgcg acgcccatcg agaatgctcc
     5101 cctgccaatg ccgtacccta ccgaaaccgt ggtaggaggg attcacctct tttcgctgga
     5161 ccttgagcac tttgaggagt tttgaccatg aagctgatca ttgccatcat ccatgacgag
     5221 gataatgacc gcgtgtcgac aacgttgaac gctgaaggca tccgggcgac ctttattgcc
     5281 tccacagggg gttttttgcg cagcggccgc agcacgctgc ttgtcggtct ggaagaagaa
     5341 aaagtccccc gcgccctgca aatcctgcgg gaaaactgta cgactggtaa ggaaggcaag
     5401 gagaaaaaag ccatcgtttt cgtcttgaac gttcaggatt acatccaggt atagtttgta
     5461 attccgtcgg aataccacta aaatcaggtg tctatcttta atttgagaaa gaagggaata
     5521 tgaatttcgg aaaggcattt tcgtacgttt ttgatgacca gcgatggctg gaaaaaatta
     5581 tcatcccgat tttgtacagc ctgatcccgg tggtgggttg gatggtgatg atgggttacg
     5641 tgatgcgcac gatccgcaat gttgctgaag gcgtggagtt cccgctgccg gtttgtgatt
     5701 ttggcgatga cctcgcgctc gggttcaaga tgtttgtcgt caatctggct tacgccgtca
     5761 ttccgttgct gctcatgctt ttgttaatgc cgctgactta cgcggtggag tcgagcaaca
     5821 gcatgccggt tctggctatc cttgggctca tcattctctc gcttttcctg ttcgtctacg
     5881 gcatcgttta tagtctgatt ttccccatta tccaggcaaa tgtggccatc aagaacagca
     5941 tctcagccgg cttcgaattc aagcaaatat ttggcatgct caccaaaaac atcggcgcct
     6001 ggctgttggt atacgcgggt ctcctcatcg gcggccttat cgccccgctc gggtccatcg
     6061 ctttctttat cggcgttttc ctgaccagta tgtactcgca gttaatgacg gcgcatcttg
     6121 ccggtcaggc ctacgcgctg accaaataga agtttcggta tttctttctg gtactgccgc
     6181 cggagtggtt cttaccactc cggtttttct ttatgttctt ccacgtggta aatccaaacg
     6241 tccaacactt tttcccgcca gaggcttggg cgtgcgccca tcttcgcgca gagatggctc
     6301 aggaattctt ccgggcgcgg gagctgctcc cagacctggg gtaaaaaagt ggcgcgtcgg
     6361 cggccgtccg cgagaatgac gccgtcctga tggggcttga gcaggcgcgg caggtcctcg
     6421 ggatgctcat aaggcagcgc caccggttcg gttaatctgg aaatttcaat ctcaatccga
     6481 ggcaattcgg cgggctcgag cgggggaaag cgcgggtcct ccaccgctgc ctgataagcg
     6541 cgcagccgga cgtcttccgc caggggctgc caggcttcca ggctgccgat gcagccgcgc
     6601 agctgcccgg atttggtcag ggttacgaaa accgcgccgt cttccagcag ccttgggggc
     6661 aggtcggcgc ttggcagagc cgccggtctg cgtccgttcg ctgccgccag aaccgcttct
     6721 ttagcgatgc gcatcagcgc atcccattca tccggggtta atttctgtgg cataagaatt
     6781 gcacctttct tgtgaacgag tttacttcgc tctttttatt atagtaacca accggtgaaa
     6841 ttccaaaccg gagcaaagtt gcggcgaata aggtaagatt aaacggatga gcaaatccat
     6901 gcacgaaatt gacgataagc ccggctttca actgccgttc cgcaagcacg aacaggtgag
     6961 tactccaacg ccggctcctg cgcccaagac cgccggtccc aagataaaaa ccagcgcgac
     7021 ctggcaagcc ttgcgggtgg tgttgggcgg cgcgctaatc gtcgccacgc ttttcacgct
     7081 ttggactccc tccagcttgc tggcggaaag cctgcaggaa aggatggcgc gcgccttggc
     7141 cgccagcggg aatgccagcc tgacccaaac agccgcggcc ggaatgccgg caggtttccc
     7201 ttccaacaaa atcggtatcg tggtggggca ccggggcaac gacagcggcg cggtctgccc
     7261 gaatgggctg accgaggtgg aagtgaacag caacgtggcc gctttcgtgc agcagaaact
     7321 cagcaaaatg ggttacgaag tggagctgct ggatgaattt gatgcccgtt tggcgggcta
     7381 tcaagctgcc ctgctcattt ccatccattc ggattcctgc gagtatttca acgataacgc
     7441 caccggcttc aaggtcgccg cggcgctctc aacttccaaa ggggataacg ccacccgtct
     7501 ggtcagctgc ctggctgacc gctaccaaaa ggaaaccgga ctgcagtacc attaccagtc
     7561 catcacggag gacatgacct cttaccacgc attcagcgag attgacccgc tgacaaccgc
     7621 ggctatcatt gaggtcggat ttatgaatct cgaccaggat atcctgacca gaaaaccgga
     7681 aaaactcgca gacggcatca tcgcggggct gcgctgctac ctggagaacg aaggggtgga
     7741 acccacaccc accccaaagc cttaacgatg gaaaccaaca gcccaaatcc ggaatcctta
     7801 atcgcccagg ccaagcaggc cgtcttgcgg ggcgaccgcg cggcggcaaa agcgctggcg
     7861 gagcaagccc tggcgctcaa tccgcgctcg gtcgatgcct tgctcatcct ggctggggtt
     7921 tcggacgcac agaccagcgt ggcgtacctg aaaagcgcgc tggacttgga cccgggcaac
     7981 caaatcgccc ggctgggcat gctttgggcg gcgcgcgagc tgcggaaaac ccacgccgcg
     8041 ggctggacgg cggaaaagcc gcaggctccg cagcctaaac tgacgcctct ggcgaacttc
     8101 cgccgccgaa agccggggtt gtggattgcc ctgctgctgg ctttgctctg cgcgagtgtg
     8161 tacgcgctgt acgctgtggg cagcttcagc ccgcgcgcgg tctctgccgg caggcagttc
     8221 acgctgtacg aatctacctc tttggtgaag cccagcctga cccccaccat cacgcccacg
     8281 cccacgcaaa cgccaactcc aacccccacc ctgacgcgta caccgacccc gacttccact
     8341 tcgaccccta cacccacgct ggagcctacc ccgaccgaag tgccggaagt catcatcacc
     8401 tatgtgccgc aggaagaaga gccgacggaa gtgtacgtgc cgccggaaga aacgcaaggg
     8461 acgcgttgga tcgatgtgga cctgggggac caggcgcttt atgcctacga ggacgacacc
     8521 ctggtcgctt cgttcctggt ctctaccggg ctgccaaata cccccacgcc gaccggaagg
     8581 ttctacgtat acgttaagct tttgtttgac gacatgcagg gaccgggtta ttatttaccc
     8641 gatgtacctt acacgatgta cttttacgaa gggtatggga tccacgggac gtactggcat
     8701 agcaattttg gcaacccaat gagccacggc tgcatcaaca tggaaacggg cgatgccgga
     8761 tggttatttg actggtctta cgtgggcatc ctggtaaata ttcataactg atggactttt
     8821 ttacacaaaa atttacgcgc agagaatttc tgaaattaat gggcctcggc gcggttgcgg
     8881 ccgcgctccc caaccgcctg gtgaaggctg ctttaacgga ttttaccgaa ggcacttggg
     8941 cgcgaattac ccgctttcgt ctgtacgtat tcgcggaacc cagcgaaaag tccaaagttc
     9001 aggacctgct gaaattcaac caacttgtgc gcatcgagga cactctgact gtcaaggaca
     9061 gcaaagggaa cgaacatccc tggctgaagc ttggcgagga tgcgtacatc cagccggtat
     9121 ttgtgcagta tgtggaaaca caccgcaacg tcagcgatgc ccccatcccg gaaggcggct
     9181 gccttggcga gattaccgtg cccatgattg acgcttacta tactccccgc ggcacctatt
     9241 ccaagcgcca ctactactac tcctccacgc attgggttcg cagccgcgtg ttcgatgagt
     9301 ttggcgtacc ctggtacgag ctgccggacg acatgaacgg ggcgtcgtat tacgtgcgcg
     9361 cttacgccgt gcgtctggtg cccgccgaag agctgacccc gctttccccg gaagtgccgc
     9421 cggagcagaa gcgcatcaaa ctggacctga aaagcgaaac agttacggct tatgaatacg
     9481 ataagccggt cttcagcaca ttggtctcca ccggcctggc ggaaggctcc accccctacg
     9541 ggaccttcaa aacccaccgc aagcgccctt accgccgcat ggtactccaa tcgggcgacc
     9601 tgaacgtgga ttacgacctg ccgggtgtgc cgtgggtgag ctacattacc atgagcggcg
     9661 ttgcctttca cggggcttac tggcactcca actggggcca ccgcatgagc aacggctgca
     9721 tcaacatgcg ctcgcccgat gccaagtggc tgtaccgctg gtgcaacccg accgtgcccg
     9781 ctgacgtgga atacctggtc ggcgagaccg gcacgcgcgt ggacgtggtt tacgagatgt
     9841 aaatagtttg taccctttgt taacttcatt tttatacgca cctgctatac tttaagaaac
     9901 aataagtaag gaaggatgcc tgtgaacaag gctgtatttg aaggtttggt atttgaccca
     9961 tccggcagcc ccctgagcgt ggattacgtc ggcagcgaag ccgtttatat tctgaacgat
    10021 gatggttttt tgcgccacat tcccgccgca gatgtggacc gccaggtttg ggaggcactg
    10081 accgcgcctc tggtcgggaa tgaggacgcg attagcgcgc aagccgccaa gatgcttggt
    10141 caggaagata ttttcagcat cgcggtcatc cgcagccagc tggaaaacaa ggataaacag
    10201 ttcgaagcgg tgcaggcagc cggtttgccg gaggacgccc gcgcttatct gggaatggtg
    10261 ggcttccgcg taactgtgga cctgcacggc gatgtgattg acatccacca gccgggtatt
    10321 gtggaggaag acggcgatta aaaggcaggg ctctgataaa attggagata gagagccgcg
    10381 atgacagacg attacatcat tgacgtgagc gacgaaagct tcgaactcga agtcgtcaac
    10441 tactcgagac aaatccctgt ggtgatggac ttttgggcgc cctggtgcat tccttgccgg
    10501 gtgcagtctc cactgcttgc caggctggcc caggaagctg aaggcgcttt ccggctggcg
    10561 cgcgtcaatg tggatgagca aaccaaactg gcggagcgct tgaaggtgaa aaacatcccc
    10621 gcggtgaagg ccttcgtgga cggcagggtc gtttcggaat tcaccggcgt gctctcggaa
    10681 cccaatctgc gccagtttct ccagaacctg ctgcccgaca gcggcgacct gctgctggag
    10741 aagggcaaga gcctgattct cctgggggat tacgccgagg cggaaaaagc tctcgggcgc
    10801 tttgtgagcg gcaacccgcg ccatcccggc ggcattctgg cgctggcaag gctgatgtta
    10861 atcgagggta aggcgcggga ggcgaaaatc ttgctggcga acttccccgc cagccacgaa
    10921 ttcagcactg cccaacagct caagcccgtt gtccaggcct tcttgtggct ggacaccgcg
    10981 cgcgaagcac cccaggacgc gctggaagcc gcctatcgca acagcctgaa actcgcgcgc
    11041 agcgggaaaa tcctgctcgc gctggacggc tttctggaca tcctgcgcaa ggataaagac
    11101 taccgcgacg atgaggtgcg ggaagtgtac ctgggcctgc tggaagtgct gggggaagag
    11161 catccggagg tgcgccaata ccgggcggac ctgaccaacg tcctttttta acgcaaaccc
    11221 ccgcttgttg cagcgagggt ttttttctgt gtcaggcagc gccttgggct gcttgccggc
    11281 gaagggaatt cagggccgca tccgcggcag tttatccgcc tctggaagcg gaaattgctc
    11341 tctgcattcc cgcgacgagc gtcggtgcgt cctaaatttt gccaggctta cgcgctgtcg
    11401 gtttggttca acaggtaagc catcaccgag cgaatggcga ctggttcgcc gtgcgcggtg
    11461 ttcaccacac aattttgctc ggcctgtgcc agggcttcct gcgaagcgcc ggtcttcgcg
    11521 tcaattccgg cggcgatttt tcgggcgccg atgaagtttc ccacgcttac ccttcttaac
    11581 atgccgcgga agtccagcgc gttgccggcg gaacagccgg ggttttcaca gaactggcag
    11641 cggaacgcgg cgcgcatgac cgcttgctgt tccatgggat aatccgcgaa aaggcgttct
    11701 ggagtgaaag gcttgtccac gatgcggacg taattgggca tccccggacg gaatttgaag
    11761 ggcgtgagcc cgcgcttttt caacaccgcg ttggccgcgc tgatccctga ggtggtcacc
    11821 gaaggcgtgc cggtgccctg cacggtggat tccccgcagc agaacaggtt ttcccactcc
    11881 gtgcgcgtgt gcaggcgctt cagcatgtgc tgcccaagca tctgttttgg tccggccacc
    11941 gcaccgccgt ttttgaggca gtagcgttca atcgtgcgcg gggtggcaag ttccgaaaag
    12001 cacaggccgg cgcggaaacc cgggaagcgg cgttccaaga cctgcgtcag gcgttccact
    12061 tcgcgggctt tcttttgttg gtattcctcc gggctcagtc cttgccaggt ttcaaagctc
    12121 ggaccgatgg ccgcgatggt atggtactgc ggcgggcaga gggtatggtc gtcgatgctg
    12181 aagatatagg cagtcacttc gctctcatcc agcaagtccg ggttgcccac cagcatctcc
    12241 accggcgcgg tatcttccgg gatgacggtc ttgtccacca gtgcgtagag aaccacgctg
    12301 gggtaggtgg gcgcctgact ggcggcccac tgcctgcgcg cggctgtggt ctcggcaggg
    12361 tcgagcagct tgccgtacag gttccagact gtgccggaat agatgatatc ccgggcgaag
    12421 agctgcctgc cgtccgtcag ttccacgccg ctgggcttgc cgtcctggaa catgatacgc
    12481 gcgacttccg cttcgggcag catgtcaccg ccgttctcct cgatgacttt ttccagtttg
    12541 ccgggcaaaa agagcgtgga gccggccggg taatagctgc cgccgacgtg gttatccaca
    12601 aacatcacgc tggcaagcac tgcgggcgcc tctttgacgg ttgcgtagca ataggtggag
    12661 gtgagcttat caaaaaactt gaaaatctca gggtctctga agtacttccc aaggagcgcg
    12721 tcggcgcctt tgttcaggta gctgaaaaag cgcaggaaag aaatggggtg cttgcgcagc
    12781 gcaggcaggg attcctgtgg gtcgctctcg tccgcgctgg tgtaactggg gttttccacc
    12841 attacatgct ggtacatgcg cagcatgtcg tgataaaagc ggcggatgtt cgcggcttct
    12901 tttggaaata ccccggaaag ctcgacgatg aactgctcga catcggcaaa gaagcggatg
    12961 cgctgcccct tgaaattcac cacgtataac aaatcgtggc gaatcatatc gataggctct
    13021 tccaggcaat tgaacaggaa gcggtgggag ttgtagccat gctcgccaaa gccgtagagc
    13081 atggcggaac cctggtcaaa gatgaccccg tcgcgcttga agatgccgca ggagccgcct
    13141 ggattataac ttttatcgat gaccagcacc tgcagcccgc gtttggcaag caggctggcc
    13201 gccgataacc cggaaaggcc ggcgccaatt acgatgacgt cgtaaaccat tacttgtact
    13261 cttggctgcc taagcgcggc gctgccggac taaagctgcg cgtcctgcgg cggcgtcaag
    13321 ggcttggtgt cctcagacat ctccggcgga ttgataccgt ccgtcgggct ggggctctcc
    13381 acagctgcgc cagcttcggg ctccggggca ggttccactt cgggggcagg ctcgggtggg
    13441 gcaatcggtc cgggcatcgg caaaggttcg gcggctgggg tggggatggg ctccggcatg
    13501 gcagtcggct ggggttcggg ggcctctttc tttttgccga aaatcgccat gaacagcgct
    13561 accactaaca aaaccaggca ggcggttacg gcaataatga ccgcgaggtg ctgcgccgcg
    13621 aagccagccg gctcgggaag gaggtgtttg tagagaattc ccaaatatgc ccagatcggg
    13681 accagaccgt aagccacgtc gcggtttaga atcgtcgtga gcccggcgat aagcgtgccc
    13741 accagcagaa tcactaccgt ccagaatgac tgctgcaagc caagaccgtc ccagccgatt
    13801 tccaccaggg tggtggtcac attggcgatg gtggcgactg taatccagcc aaagtacacg
    13861 ctgaagggag ccctaaccag cagcttttcc ctgcgtgtgc tggcgccttt tttcaacatc
    13921 agagcgatga tgatgagaca cagcagaatg accagcatcg ggatgttgga ccatacaatt
    13981 ctctcgtaat gccacaggaa aatccagact gtgttcgcga gagaggagat accgaagaag
    14041 atgttgacct tcgccagcag cttggtgtta ttcgcttcat tcttggcgcg cagcagcccc
    14101 atttggtaaa gcgtgtagag cagcagcagc aggtaaatca cgccccagat gatgaaggtt
    14161 tcgccagccg gtgcgaaaag gttcgggcgg ctgtcggaga tctcgccggt aagtttgcca
    14221 ttgattggca ggatattcgc cagggcattc accgcgacca tgatgagata ggtgaccaga
    14281 gcgaagaaat tgtgaaactt aagtttgggt ttgctttcca tagttgcgcc tgtatattcc
    14341 tttcatgcta ccttagccag gaagcgattg ttcctcaaag ctgaatgcgc ggggaacata
    14401 ccgctcaggt aatattttac ctgaaaggct aaattttgga tacgcatccg gtggttaagg
    14461 gacagtcctg aaattcccgc cggcggtcac gcggttggcg ggtaaatgag gcctttaaac
    14521 aaagtacaaa cattacacaa aagcttattc agacgattac ggagggttaa ctgaccaggc
    14581 tgctgataaa acaatattct ctttcgcccg cgcagcaaaa tggaagacaa gtacaaaggt
    14641 ttatatcgat ttcatctggt cataggcttt ttacacctca ttcagggcgt cctgttgatt
    14701 gtgcccggca atgagaatcc ctatccaatg gttgccattt ttctcaggcc tactcccgaa
    14761 acccgcaagc tggtaccaga ccccaggctt ttccgggaac ttccgtttgg cgcggcagca
    14821 gcgattttcc ggttgatttc tgacgcgggg cattcacaat cggctgcaaa cgctgcacca
    14881 gccctcttag gcgcaagagg aatcccatcc gcttttatac ctgcgtcctc agctcacggg
    14941 cctgttggtg ggtatccggg atcttccgac aacggagcgt ccggctttcg cgggttgctg
    15001 gactttcgcg ggcggttgga gtgcatgcgc ctggaacgca cgcgccaaaa aaaaacggaa
    15061 cgacccgcgc aaggtggcgc ccgggaaaat ctgcaaaaac ctaaggttca gagtgtgttt
    15121 cgcgtgtcgg gcttattaac gcttggaacg cgctgagccc gataagcttg acggcagcgt
    15181 cgccggtctt ggcggccaac tgctccgcgg cccatttgga attgccagtg cctgaaaaat
    15241 cataaatcag gcctattcct ctgtttggga gagatttcca agttcccgga agattaatgc
    15301 tgacgcgatg cgcagagcgc taatgcggtt ctttagcagc gtgtgctggg atgtgccggc
    15361 ttgaaattta ccctgcactt tctcgcactt atccagcaga gagttgattg agcgcagagc
    15421 ttgggttagg gtttcctggt cgggattgtc cataagtatc ttcggttctc actgctgaca
    15481 attttccacg tccttgacca acacagccac cgcctcgata tggtaggcct gggaaacttt
    15541 gggacatcgt gataatcgtg actgttccaa actattcctg aattttcccg attgtccaaa
    15601 ccatattggt tgctaacctt tccagaacct ctctgccgta cgcgtccttt tccacccgat
    15661 cagtatcagc atccacaatc atgttccagt aattgctgct gaccacaagc atttcggata
    15721 ttgtaaagta cttattcaag cgatccaggg cggcagtagc cccagcgcga tagcaattga
    15781 ctaccgccgc ggcaggctta tgggcgaata tattcgcttg attggtggca gcatagaaga
    15841 cgcggtccaa caaggcgcac agcgcgccgt ttggtccggc aaaatagaca ggggaaccga
    15901 cgataatgcc atcagcattg atgatcgctt caatcagtga attacacaaa tcatcctgat
    15961 aaacgcagcg attcgtcgcg gcgcaagaat cacacccatt acagccgcgt aatttctcag
    16021 tccccaactg aaatattgta gtcgccgcct ggtgtttttc cagaacagcc tcgatgactt
    16081 ttagcgattt gaaagtgttt ccatctttat gcgggcttcc gttgatcagt agaatgttca
    16141 ttttatctcc cagaaagaca tggacttact ttgcacggga taatactgcg gccgtttcga
    16201 tgtggtaagt ctgcgggaac atatcaaagg cgcgcacgtc ccgcaagctg taccccgcgg
    16261 cgcacaggtg cttcaggtcg cgcgccagcg tgcttgggtc gcagctgatg taaataatct
    16321 gcccggcgcc cagctcctgc agggctttgc gcgcggccgg ccgcaggccg gcgcgaggcg
    16381 ggtctagcac taccaggtcg acccctttca ggtcggccgt cagccctggc agcacctgct
    16441 cgaccgcgcc ttcatacagg ctgacgtggt cgtacgcgtc caggttgttg gcaaagtcga
    16501 agcaggcgga aagggagctt tcaatcgcga ttaattcgcg gcattgctcc gccagaaacg
    16561 cgctgaagaa gcccgcgccg ctaaagagtt ccagcacgcg caggtcgttc cgcgcgggga
    16621 gcagactgag cacatgggag agcatctgct ccgcgaccgc gagattcacc tggaagaagc
    16681 tctccggtga gacgctcaac tgttttccca ggacctcata gaccagctgg tcctgccccg
    16741 cgagcgtgat tgcctgcctg tccggcccga ggtaagagac ggaagccggc aagtccaggc
    16801 tcacttcagg ggcaagctcg tccgggcctt ccagcacgat gaactgctcg ccctggctgt
    16861 cctgccgcag tacgacgcgg ctgagacctg cttctgggct gaggtccaac tgcgcgcgca
    16921 aggtttcaag atccgcaagc ggaagcaagc actgcgaaat cgggacgacc tgcttgcccg
    16981 tcaggtccat gtacccaagc tgccccagcg cgtccagatg gaactggacc tggttgcggt
    17041 agtgccagca ctcagggctg ggcatgatgc cgctgagggg cggattgggg atgccgccca
    17101 ggcgcgccag ttgatccgcc agaatctgga gtttgagctc cagctggcgg gcataaggca
    17161 ggtgttggta gtggcagccg ccgcacgcgc cgtagtgcgg gcaaggagga gcgacgcgct
    17221 cggcggatgg cccgcgcact tccacagcgc gcccgcgcgc gaagttctgg ttatcttccc
    17281 aaatctcgac cagaacctct tcaccgggca gggtgaaagg gacgaagatt gcgcgcccat
    17341 ccgccagcct gcccatgccg tcgccgccgt acacgcattt ttctatcaag acagtttcgt
    17401 tcatgtctca attgtacccg tgcgagcgcg ggctggaaga ctcgcggacg aaacttcctt
    17461 cgggtatgct gggcagtaca accgctccgt cgcaggctta tcctcggacg gtcaggttag
    17521 cgctttgcgc ttcagcggag aatccgccag ggcgggggct aagtttttcc cccgcgggaa
    17581 ggctgcgcgc gaggccaaaa aagccccaaa aacccgctct gcgggtatac taagggtgag
    17641 ccgattggcc catcaaacaa cgctgagagc ataccaaaag gagagttgct taagatgaaa
    17701 tacggacgcg tttacaattt ctctgccggc ccaagcatgc ttcccgaagc agtcctggag
    17761 gaggtccaga ttgaactcct gaattaccag ggctccggaa tgagcgtgat ggaaatgagc
    17821 caccgcagca aggtctttga ggatattctg catgaagccg aaaaagactt gcgggaattg
    17881 atgaatattc cggataacta tgaagtcttg ttcgtccagg gcggtgggac ggtccaattc
    17941 gccatggtgc cgatgaacct gatgaaaaac ggcgcagcgg attacgtcaa taccggagcc
    18001 tggtccaaaa aagccatctc tgaggcgaaa aagttcggga aagtgaacgt catcgccagc
    18061 agcgaggacc gcaatttcag ctatattcca gacatgcgca atgtcagtat taccccggat
    18121 gcggattacg tctatatctg cgagaacgaa acgatcaatg ggaccacctg gcagactctg
    18181 ccggataccc aggggaaacc gctggtgtcg gaccaaagct cgatgttcct gtcccgcccg
    18241 tgcgatgtga gcaaatacgg cctcatctgg gccggcgtcc agaagaacgt aggccccgcc
    18301 gggctggcaa tcgtcatcat tcgcgaggac ctcatccgcc gtgaagacct ggaccccaaa
    18361 gtgccgacct atctgcgtta cgatacgcat gccgataacg acagcctcta caacaccccc
    18421 aactgctggg cgatttatgt gtgcggcaag gtattcaagt acctcaagaa gcttggcggc
    18481 ctggaagcga tgaacaagct gaaccaggaa aaggcagccc cgctgtatgc ctacctggac
    18541 cgcagtgaaa tgttccacgg cacagtcgag aagaaagacc gctccctgat gaacgtacct
    18601 ttctcgaccg gcgataagga gttggatgcc gcggtggtga aggcctcgct ggaagccggt
    18661 ttcgacaacc tgaagggaca caagagcgtt ggcggcttgc gggcttccat ttacaatgcc
    18721 atgcccaagg aaggcgtaga cgccctggtg gaattcctgc agaaattcga agaggaacac
    18781 ctgaagtagc ctttaggcgc gttggcgaaa acaaaaaatg caccgcatcc cgcgagtaaa
    18841 accgggtgga tgcggcgctt tttcattggt tgtggaagct gccgtggagt ggtcaaggaa
    18901 caaggcatgt tgtgctttgg ttccggacag tttttgacgc tggagcaggc ttttttcagc
    18961 ttgcacaaaa tgctggatga cgctataatg ctaacctcac gggcggttag ctcagttggt
    19021 tagagcgttg cgttgacatc gcaaaggtcg taggttcgag tcctatacca cccacagaca
    19081 ccctgcaagc ctgatgcagg gtgtttttgt ttcacttacc tgccattttg gcacacaata
    19141 ttgccggttt ccggtaaaaa accacatgaa aacgtagaaa atctcggtaa agact
//
LOCUS       DAOQAD010000029        19094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_3006_length_19094_cov_5.918746, whole genome shotgun sequence.
ACCESSION   DAOQAD010000029 DAOQAD010000000
VERSION     DAOQAD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19094
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3006_length_19094_cov_5.918746"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1737)
                     /locus_tag="PKL60_03900"
     CDS             complement(259..1737)
                     /locus_tag="PKL60_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNW95312.1"
                     /translation="MMTVRKSHIKARGFAFLLALVLFTSACTEARPTYILPAAQQTEE
                     AEQPTSTPFPARPVYAPGTLVDYTAQSGDTLLLLSARFGATVQEILWANPEIPESTAT
                     LPPGFPMKMPIYYKPLWGTSYQIIPDSAFVYGPDLIGFDLRAYVESSPGWYKQYGSYI
                     QAEYKDAADLLLWLGENYSINPRLLLALLEYRAQALSNPTRDRASELNLLMPEEVYTG
                     VYLQLSHSADLLNDGYYRYRQGELGSITHLNGEIENIDPWQNAGTVALQYYFSLFLDG
                     EDYKRAIGPDGFAKTYNDMFGDPWQGNTTVLTGSLTQPQFILPFRLGTTWSYTGGPHT
                     GWGNLKPWAAIDLAPPVGSKGCVGTNEVAVAVADGVIVRTGPGTAVLDLDGDGDERTG
                     WNVFYLHIAQEGRVTVGEVVKQGDPIGHPSCEGGQATGTHLHIARKYNGEWISATGFL
                     PFNLDGWTPVEGSRAYLGQLVRGDEVVIASDESPTSSMITRQ"
     gene            complement(1784..3373)
                     /locus_tag="PKL60_03905"
     CDS             complement(1784..3373)
                     /locus_tag="PKL60_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95313.1"
                     /translation="MAARRRRKTQRLVVAALAFTICGGAVLALPAEAPGKVVANIAAD
                     ETALPLSLPEQAPDLLEAPFAAEDSAGSTVLVNQPPAVLENQSSAVAASQSAPSLYYA
                     QSGDSLDVLALRFGVPVNEISSTGELLEHGFISEGQLLLIPMRLENISDGLRLFPDAE
                     VVNSPSALDFNLEDFISQAGGKLASYQESVYANGMMTGAQMIRRVARDYSIHPRLLLA
                     LLEYESGWVYGSPATEEQRYYPLGLPESGQSGLYKQLVLAAGKLGTGYYGWREGTLLA
                     LTFPDGSSLRLAPELNCATVAWMYYFAQTRNQDAWSEALYSEHSLLGTYEAMFGNPWL
                     IAQEYASLFTPGMEQPALDLPFESGIVWSYTSGPHAAWGAVEVRAALDFAPPADAPGC
                     LPNSTWITAAAPGFVVRSDNGTVVVDLDGDGNEETGWNIVYLHVGTTHRIPLGAWVEK
                     GERIGHPSCEGGISTGTHLHIARKYNGEWIPADGPLAFNLSGWTAKAASVPYQGWLIK
                     GEEVVKSNRYSATPAHIARWD"
     gene            complement(3402..4988)
                     /locus_tag="PKL60_03910"
     CDS             complement(3402..4988)
                     /locus_tag="PKL60_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HNW95314.1"
                     /translation="MEPIHRFSHLKSSARTIRLHGCAFVLTGLLMAGCYRPIANYDPW
                     RVPQSSLQGATPTRMNDQVLTLTPAKPRTTITPAPTGPAPTPNQPALLPTLRVQTDEY
                     TVVSGDSLARIALRHQVSVGQILKANPDLKDPNLIEPGQKLLIPPASANELATNFKII
                     PDAELFYSPSAVGFDTLAVTMQFAGKLSSFSETLEDESTLGGAEIVQRVADEFSVNPR
                     LLLAILEYRSGWVTSAGKAGISEDYPLGLQDANRKGLYKQLSWAANEVTRGCTLWENS
                     AVAVWTLSDGAVMRADASINAGTAGLQYLFSLFLGKEEWKEAVSAAGFYADYSRLFGF
                     PFAFSIEPLVPENLTQPELELPFAAGDTWYFTGGPHVGWGTGSAYAGIDFAPPGEEYG
                     CYESQTPVTAAADGLVVRSGNGVLVQDLDGDGIEQTGWSLLYLHLATEGRAALGTYLR
                     QGDLLGYPSCEGGYSTGTHLHLARRYNGVWIPADGRVPFVLSGWTCESAGIEYDGYLV
                     KNGQTIEAFNGRADFNAIGR"
     gene            5158..5242
                     /locus_tag="PKL60_03915"
     tRNA            5158..5242
                     /locus_tag="PKL60_03915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5192..5194,aa:Leu,seq:cag)
     gene            5342..5743
                     /locus_tag="PKL60_03920"
     CDS             5342..5743
                     /locus_tag="PKL60_03920"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNW95315.1"
                     /translation="MKSFWEKIKQINWADKRVFLIAAVVLLVFLMMDFNNRMVRSLQL
                     EKQEQQLNARIAALEQTKQRLEADIAYANSDRAVEEWAREEAKLIKEGDVPIIIRAQS
                     SAAAAAPTPAPQAENPELSRLEIWKQLFWGE"
     gene            6000..6326
                     /locus_tag="PKL60_03925"
     CDS             6000..6326
                     /locus_tag="PKL60_03925"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HNW95316.1"
                     /translation="MNLDELTQKMHALVESKGWYAEDSVKQQTPRNLAISLVLEAAEV
                     LELFQWQAECEDREALAGELADTALYLLQLASVSGIDLEAAVLTKISQNQTRLWPEHL
                     GKELKA"
     gene            6323..6868
                     /locus_tag="PKL60_03930"
     CDS             6323..6868
                     /locus_tag="PKL60_03930"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNW95317.1"
                     /translation="MIIAVFGSARPVEGDALYEDARRLGALLAQQGHTLMTGGYCGLM
                     EAVSRGPAEAGGSTIGVTCAEIERHRPGSANPWVQVNIRTQTLNQRLEVLTRQPDAMI
                     ALPGGIGTLTELSLALNLSVISANSAKPIVLLGPVWRAVLENFYILNDGLTSQEDLAR
                     VRFCVTPEEAAEFFHNNTNRK"
     gene            6872..8305
                     /gene="proS"
                     /locus_tag="PKL60_03935"
     CDS             6872..8305
                     /gene="proS"
                     /locus_tag="PKL60_03935"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNW95318.1"
                     /translation="MEKKLPTRQENFSEWYNQLVLRAELADYAPVRGCMVVRPYGWAL
                     WENIQKALDERFKATGHVNAAFPMLIPQSFMTKEKEHVEGFSPELAVVTYGGGEKLEE
                     PLVVRPTSETIIGTMYAKWIQSYRDLPVLINQWANVVRWEMRTKLFLRTLEFYWQEGH
                     TAHATEQEALDETERMLQVYADFARTEAAVPVIPGRKSESEKFAGAVATYSIEAMMGD
                     TKALQAGTSHFLGQNFAKAFDIKFLDESNQLQYCWTTSWGVSTRFIGAIIMTHGDDQG
                     LVLPPRLAPIQVVIVPIYRKDEEKSAVMEAVNAVQAQLKALRVKVDDREGLTPGFKFN
                     DWELKGVPLRIEIGPKDVQNGTAALSRRDIPGRDGKTFLPMASLESSVPDLLQTIQEN
                     MLTKAAAFRDSHIFEVNDYESFKQVVADQGWAYAWWSDDREAEARIKEETKATLRVYA
                     SDQNLGEGRCFYSGRKTNKKAYFARSY"
     gene            8315..9130
                     /locus_tag="PKL60_03940"
     CDS             8315..9130
                     /locus_tag="PKL60_03940"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNW95319.1"
                     /translation="MTAIDLTRLQKQIGDLVAVFSDPAVFIKTLQEILGFYERRAYRP
                     NRQLVPKTFMRSYNLPPQVLPLIEIGLKEQAGLHPSDALALADALWQSPYYEARELAA
                     AILGQLPADFSAQVLTRIEEWVSAPLDRAALQTLLEKASPPARLAGSWEGFIEKLLGS
                     PIIRMQSVGLLALAIDLEQRPLSKYTGIFKLVRPFIQAGDERLEMNLSLVVKSLARRT
                     PNESAYLLKQILADSSGSGIERRIREYLAYFPPETAAGLLEAVKTHQKIHKAD"
     assembly_gap    9268..9367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9702..11774)
                     /locus_tag="PKL60_03945"
     CDS             complement(9702..11774)
                     /locus_tag="PKL60_03945"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNW95320.1"
                     /translation="MDYQTWLYVGIAGGIISIAVAVYLYFWVMRQDPGSERTQQVGSW
                     IRSGANTYLKRLYTSLATLALILGIIIALIFSFNFETIGTTGVTIEPGSGVIMAACFI
                     IGALCSAVAGYMGMRVAVEANVRTATAAQKSLQQAFRLSFYAGAVLGLAMVGLAVLGM
                     SIIFILTGEAHAVLGFSFGASTLALLAKAGGGIYTKTADIAADLVGKVEIGIPEDDPR
                     NPAVVADNVGDNVGDVAGMGADIFDSYVASAVAVMLLGASFSTGMQYKYTVIPLILCT
                     LGTFASLLGIQFVKVKESGKPGPALNRGTIITCIIFVGALLVLSLLKVIEPGIFWSAA
                     VGIVTGVIIGFTSDYFTNDQYKPVKETARVSTSGSAVTIITGFSYGLISIVPSIIGVV
                     VATLISYSVALGDGLPGIYGIGVAAVGMLSISGMIVSADAYGPIVDNARGIAEMAGMG
                     QEVIERSDVLDSAGNTAKAITKGFSISAAALTVLALFAAYAEVVKANGIDLNISLREP
                     IVIAGVLLGAMTPPLFSALLMLSVTHNAFDMIEEIRRQFHEKPGILAGTEDPDYDRCV
                     GLAARGALSSLLLPAFLAVGLPLVIGFVLGPDALGGFLGGAIFTGVIFALLMANAGGL
                     WDNAKKYIETGKFGGPGSDAHKAAVVGDTVGDPFKDTAGPSLNTLITVMSLVSSLFAP
                     IIAVFHLF"
     gene            complement(11811..12575)
                     /locus_tag="PKL60_03950"
     CDS             complement(11811..12575)
                     /locus_tag="PKL60_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95321.1"
                     /translation="METHFYLSLLPEALIVSMLNPEEFGSYYAVGSSKKSRGQAMFFE
                     VDPNYQNSYFHLDEALKRCVPHEDGRPKSSIYVSVYRVLEHIDPSALLDLYLTTQDGR
                     TLALKSSEIIPDGDEGLHLYQEIAPVTPLVVSRLDPLSFYDLIVNNPTSLISVPAITF
                     AELQLGELASDPEMGLLNNLPYSNPDHLRQCLSDVKTKFVSTKMVDRTHSPVIPYRTI
                     KNGFFVGNQEKLRYYPMPSAETLRAENYRWFRSASM"
     gene            complement(12734..13141)
                     /locus_tag="PKL60_03955"
     CDS             complement(12734..13141)
                     /locus_tag="PKL60_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95322.1"
                     /translation="MKNKNILLVSILLAAVLLLSACGTKEEGSSINLPAVSVGESTEV
                     SPAQSQSSGMYPAGEAGQSASAAYPAGQSAALSEADVEALLIEKLGGHHALDWVLQFD
                     KTYAEWDQFLSNHHGVSFTAEEKDAVINYLISH"
     gene            complement(13237..14679)
                     /locus_tag="PKL60_03960"
     CDS             complement(13237..14679)
                     /locus_tag="PKL60_03960"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNW95323.1"
                     /translation="MNLSRRIYRLSPSQFSAETIAVTFAKTSRSPEAFDQIAAELDAE
                     KSSKFSEKWIVGYGHSSVAEHAVLHLALENISRLAIETLESNRLASYTEKSTRYQEWN
                     PEAWLLPPEIAGSRFEERYRRVMGLLFHGYAEAMTALRAWSESATGRLPEESEKAWQN
                     RARVKAVDVGRFFLPAASMANVGVTINARALEYAIKKLLSSELEEVRQIGSEVKTVAE
                     QELPTLVKHANPIESLPGIREGLRAEAGRIAVLPGADWCRLLGAEPAGEEQVLAAALL
                     RFSGRSYAECLAHVRGLADQEKAALVQEILGHLGDFDTPLRELEYAHYDFELVMDQGA
                     YFEFKRHRMMTQTAQPLTTRLGYAVPRAMSLAGVESLYRQAMDAAAELYEALSGVHPE
                     AAAYVVPNGYNRRVLCRMNLRELFHFTKLRASANAHFSIRRAALRMAELAVGIHPLFC
                     SALCLPVGENADWLTDEYFTEVAVLPLGSS"
     gene            complement(14648..15598)
                     /locus_tag="PKL60_03965"
     CDS             complement(14648..15598)
                     /locus_tag="PKL60_03965"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNW95324.1"
                     /translation="MTRFYEDWPAEGKLPLWITIGNFDGLHRGHLALLDRLKERAAKE
                     GSQTAMITFQPHPRVVLQNIRGPFYLACEAEKKLLLEQSGLDFVFTLRFDRALADLSA
                     GQFFALLLEHLDLRGLTVSENFALGKDRTGTLRQISGLCAENGIAMDVVPPFCLDGIP
                     VSSGRVREALEAGDVREAARLLGRPYFVKSKVVEGKHLGSKLGFPTANQVVFPQKILP
                     QYGVYATRACFNGEKLLGVTSVGVRPTFENTTRANIETLLLDFDDNIYHEDLTVEFIE
                     HLRSEIKFERVEDLIRQIEQDKQEARRILTHESFQENLPA"
     gene            complement(15681..16607)
                     /gene="truB"
                     /locus_tag="PKL60_03970"
     CDS             complement(15681..16607)
                     /gene="truB"
                     /locus_tag="PKL60_03970"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNW95325.1"
                     /translation="MSDEMMESRENLANSVSGVLVVDKPVGLSSHDVVQIIRNGTKIR
                     RAGHTGTLDPRASGVLVVLLGPAVRLSEYVSASEKRYQAVIELGITTDTYDLEGRVMR
                     RSPVEISYEEFEEALQGFIGEFEQKPPAYSAIKIKGEKAYELARRGEEVDLEPRMIQV
                     HELELLDWDPPEAVIDIQCSSGTYVRSLAHDLGEKLGCGATLTGLRRTKNGSFGLRDA
                     VSLRKLQEAFTNGDWYQYLIPAAEALGDWYTVELTVEQVDEMRHGHRVPAVDVVEPGK
                     WARAVSQEGELVALVEYDSATNEWQPRKVFFL"
     gene            complement(16640..17596)
                     /locus_tag="PKL60_03975"
     CDS             complement(16640..17596)
                     /locus_tag="PKL60_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNW95326.1"
                     /translation="MTETVDLQIQKLLKSARSVVIAAHIRPDADAVGSLLGLGLPLLA
                     EGKQVQLVLQDGLPEKYAFLPGAERVARSIRSGFDTLITVDCSDPKRLGSVLEGLPIP
                     DLNIDHHQTNLNFGKINLVEADAPATALVLAEHLPRWGLTVDANAAQCLLTGILGDTI
                     GFRTSGTDARTLRTAADLMDRGANLPLVYRQTLLSRSIPELRYWGQGLTRIQHEDKLL
                     WTVLTLDDRIKAVYPENDDADLINLLSSVSGLRVVVLFIQQTQDRVKVSWRSAEGIDV
                     STIAFEFGGGGHSAAAGADVEGSLEEVVSKVLHRTRQLLEAS"
     gene            complement(17589..17990)
                     /gene="rbfA"
                     /locus_tag="PKL60_03980"
     CDS             complement(17589..17990)
                     /gene="rbfA"
                     /locus_tag="PKL60_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNW95327.1"
                     /translation="MPTPLRMKRINDRVKEILSLTLLNKIEDPRLTGVNITDVKVDRE
                     LDFANVYVSAPEGRVREREVLEALNHARGFLKFELANEIDLRVMPRLRFFWDPTPERA
                     DRIDTLLAQLRDESDQVGEAADEGEDQESND"
     gene            complement(18021..>19094)
                     /gene="infB"
                     /locus_tag="PKL60_03985"
     CDS             complement(18021..>19094)
                     /gene="infB"
                     /locus_tag="PKL60_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNW95328.1"
                     /translation="VPVSAKTRKGLDDLLETILLVADNMIIKANPKGACIGTVVEAKL
                     DKTTGPMATLLVQNGTLKVGSVVVAGESYGKIKAMFDQRNKRIEKAPPSTPVVVMGLD
                     HLPDAGDLFQVVENEREARAIVESRKAEREAKRNIKRATLEELFAKVKSGETRELRLI
                     LKADVRGSLEPIVNSLEDLGKKEKDISIQVLHSETGNVTESDVALAAASNAVILGFNV
                     DVDPNTRHMAEAEGVSIRVYKIIYRMLEDVEKALKGLLEPEMVEKVIGTAQVLATFKV
                     SKAGMAAGCRVLDGEIRRNASVRVMRAGEQVFAGEISSLKREKEDVREVREGMECGIS
                     IKNFEDFEVGDTIESYIMEKFGG"
BASE COUNT         4140 a         5723 c         5225 g         3906 t
ORIGIN      
        1 cgcaacccga cagaacatgg tcgtaggtcg ggtggagcgc cagcgcaacc cgacaagctt
       61 tggtcagccg gtttgtgcag tcgttgtcag gtttcacccc tgcgcctctg cactcagctt
      121 aaacggcttg ccatcctgag cgtatcttgt catcctgagc gccagccaag gatctactcc
      181 cctgcactaa ttgcttcgcc tgtggcttag catgacaaat tcttattttc ctgaacgccc
      241 acgtgtcaat cctgggcttt actgcctggt tatcatgctg ctggtagggg attcgtcgct
      301 ggcgattaca acttcgtccc cgcgcaccag ctgaccaagg taagcgcggc tgccttccac
      361 gggcgtccaa ccgtccaggt tgaacggcag aaaaccggtc gcggaaatcc actcgccgtt
      421 atacttgcgc gcgatgtgca gatgcgtacc ggtcgcttgc ccgccctcgc aggaaggatg
      481 cccgatcggg tcgccctgtt tcaccacctc ccccaccgtc acgcgccctt cctgcgcgat
      541 gtgcaggtaa aaaacattcc aaccagtgcg ctcgtcgccg tccccatcca ggtccagtac
      601 agccgtgccg gggccggtgc ggacaatcac gccgtccgcg accgcgaccg ccacctcatt
      661 cgtccccacg cagcccttgg atcccaccgg cggcgcgaga tcaatcgcag cccagggctt
      721 caggttgccc cagccggtgt gcggaccgcc ggtataagac caggtggtgc ccaggcggaa
      781 gggcagaatg aattggggct gcgtcaggct gcccgtcagc accgtcgtat tgccctgcca
      841 ggggtcccca aacatgtcat tataggtctt ggcgaagccg tccgggccaa tcgcgcgctt
      901 atagtcctcg ccatccaaaa agagcgaaaa ataatattgc agcgctacag ttccagcatt
      961 ctgccatggg tcgatatttt caatctcgcc gttcaaatga gtgatggagc ccaattcgcc
     1021 ctgccggtag cggtaatagc cgtcgttcag caagtccgcg ctgtgcgaga gctgcaaata
     1081 aacgccggta taaacctctt ccggcatcag caagttgagc tcactggcgc ggtcccgcgt
     1141 tgggttggag agcgcttgcg cccggtattc caacagcgcc agcaaaagtc tcgggttgat
     1201 gctgtaattt tctccgagcc acaaaagcaa atccgccgcg tccttatact ccgcctggat
     1261 gtacgagccg tactgtttat accagcccgg gctggattca acataggcgc gcaggtcaaa
     1321 gccaatcaag tcgggaccgt agacgaaggc ggaatccgga ataatctgat aggaagtgcc
     1381 ccacaagggt ttgtagtaaa tcggcatctt catcgggaag ccgggcggca atgtggcggt
     1441 ggattcgggt atctcggggt ttgcccacag gatttcctgt accgttgccc caaaacgtgc
     1501 ggagagcagc agcagggtat cgccgctctg ggcggtataa tccaccagcg tccccggggc
     1561 gtagaccggg cgcgcgggga aaggcgtgga ggtcggctgt tcggcttcct cggtctgctg
     1621 cgcggcaggc aggatatagg tcggtctagc ctccgtgcat gcgctggtaa atagcacgag
     1681 cgcgagcagg aacgcgaacc ctctggcctt tatgtgggat tttcggactg tcatcatttg
     1741 aatcggcggt aaacgccact actataccac aaaccggggg ggcttagtcc caacgcgcga
     1801 tatgcgcggg cgtggcgctg taccggttgg acttgaccac ttcttcgccc ttaatcagcc
     1861 atccctggta gggcacggaa gcagctttag cggtccatcc gctcagattg aaggccaacg
     1921 gtccatctgc cggaatccat tccccattgt atttgcgggc aatgtgcagg tgcgttccgg
     1981 tcgaaatccc gccctcgcag gagggatgcc cgatgcgctc gcctttttcc acccacgctc
     2041 ccagcggaat ccggtgggtt gtgcccacat gcagatacac gatattccag ccagtttcct
     2101 cattcccatc accgtccaaa tccaccacaa ccgtgccgtt atccgagcgc accacaaagc
     2161 cgggtgcggc ggcggtaatc caggtgctgt tgggcaggca gccgggcgcg tctgctggcg
     2221 gggcaaagtc cagagctgcc cgcacttcca ctgcgcccca ggccgcgtgc ggtccgctgg
     2281 tataagacca gacaatcccg ctttcaaagg gcaggtcaag cgcaggctgc tccatgcccg
     2341 gcgtgaacag cgaggcatac tcctgcgcga tcagccaggg gttgccaaac atagcttcat
     2401 aggtgccaag caggctgtgc tctgaataca acgcttcgct ccaggcgtcc tggttgcggg
     2461 tctgcgcgaa atagtacatc cacgcaaccg tggcgcagtt tagttcgggc gccaggcgca
     2521 ggctgcttcc gtccgggaaa gtcagcgcaa gcagcgtgcc ttcgcgccag ccgtaatagc
     2581 ctgtgccaag tttgccggcg gcgagaacca actgtttata cagtccgctt tgcccggact
     2641 caggcaaacc caagggataa taccgctgtt cttctgtagc ggggctgccg tatacccagc
     2701 cgctttcata ttccagcagc gcgagcagca gccgcggatg aatggaataa tcccgcgcga
     2761 ccctgcggat catttgtgcg cctgtcatca ttccgttggc gtaaacactt tcctggtaac
     2821 tggccagctt tccgcctgcc tgcgatataa agtcctcgag gttaaaatca agcgcgctgg
     2881 gcgagttgac tacctcagcg tccgggaaga gcctgagtcc gtcgctgatg ttctccaagc
     2941 gcatgggaat cagcagcagc tgcccctcag aaataaagcc gtgttccagc agttcccctg
     3001 tgctgctgat ttcgttcacc ggcacgccaa atcgcagggc cagcacatcc aggctgtcgc
     3061 cgctctgcgc gtaatacagg cttggcgcgc tctgactggc cgccaccgcg gaggactggt
     3121 tttccaacac cgcgggaggc tgatttacca ggactgtcga cccggctgaa tcctccgcgg
     3181 cgaaaggcgc ttccaacagg tccggcgctt gttcaggcaa gctcagcggc agggcggttt
     3241 cgtccgcggc aatgttcgcg accacttttc ccggggcttc cgccggcagg gccaacactg
     3301 cccctccgca aatcgtgaac gccagagcgg caacgaccaa acgctgcgtc ttccgcctgc
     3361 gcctggcagc catcctgcgc tgcctctccg cgcgcgagca gttaacgtcc gatggcgttg
     3421 aaatcggcac ggccattaaa agcttcgatt gtctgtccat tttttaccag gtatccatca
     3481 tattcaatgc cggcgctttc gcaggtccaa ccgctcagga cgaaaggtac cctgccgtcc
     3541 gcaggaatcc atacgccgtt ataacggcgc gcaaggtgta aatgcgtgcc ggtggagtaa
     3601 ccgccttcgc aggacgggta cccgagcagg tcgccctggc gcagataggt tcccaaggca
     3661 gccctgccct ctgtggcaag atgcaggtaa agcagcgacc agccggtctg ttcaatgccg
     3721 tctccgtcca gatcctgcac gagaacgccg ttcccggagc ggaccaccag cccatccgcc
     3781 gcggctgtta ccggggtttg gctctcgtaa cagccatact cctccccggg cggagcaaaa
     3841 tcaatgccgg cataggcgga gcctgtcccc cagcccacgt gcgggccgcc agtgaaatac
     3901 caggtatcac ccgcagcgaa gggcagttcg agttccggct gggtcaggtt ctcaggcacg
     3961 agcggttcaa tcgaaaaggc aaacgggaag ccaaacagac ggctgtaatc agcgtaaaat
     4021 cccgccgccg aaacagcttc tttccactcc tcttttccca ggaataagct gaaaaggtat
     4081 tgtaaaccag cggtaccggc gttgatgctc gcgtccgcgc gcatgactgc gccgtcgctc
     4141 aacgtccaca ccgccacagc ggaattttcc caaagggtgc agccgcgggt tacctcgtta
     4201 gctgcccaac tgagctgctt gtaaagacct ttgcgattcg cgtcctgcaa tcccagcgga
     4261 tagtcttcgg atatgcctgc ttttccggcg gaagtcaccc agccgctgcg gtactccaga
     4321 attgccagca gcaagcgcgg gttcacagaa aattcgtccg ccacgcgctg aacaatctcc
     4381 gcgccgccca gggtgctttc atcctccagc gtttcgctaa agctactcaa cttgccggca
     4441 aactgcatgg tcacagcgag cgtatcaaag ccgaccgcgg acgggctgta aaacagttcc
     4501 gcatcgggga taatcttaaa attggttgcc agctcgttcg cgctggcagg cggaatgagc
     4561 agcttctgcc cgggttcgat gagattggga tccttgaggt ccggattggc tttcaggatt
     4621 tgccccacag aaacctgatg gcgcagcgcg atgcgggcca gcgaatcccc ggaaaccacg
     4681 gtatattcgt cggtttgcac gcgtaaggtt ggcagcaagg caggctgatt tggagtgggc
     4741 gctggcccgg tcggcgcggg cgtgatagtc gtccgaggtt ttgccggggt caaggtcagg
     4801 acctggtcgt tcatccgggt tggggtcgca ccctgcagcg aactttgcgg tacacgccag
     4861 ggatcgtagt tcgcgattgg ccggtaacag ccagccatca gcagtcccgt gagcacgaaa
     4921 gcgcacccat gcaagcggat ggtgcgagcg ctcgatttga ggtgcgaaaa gcggtgaatg
     4981 ggttccatca gagcttatga tgcaatattc gtgccacacg ccccctaaac tgcccgcgca
     5041 tctcgcgctg cggggaggtt actgcccaaa tatgtaagca gattcaaagc cggagcaagc
     5101 cgcggaaaga gatttcattt gacaaaatgg caggattgga taaaatacta tcctatcgcc
     5161 ggagtggtgg aactggcaga cacgctacgt tcagggcgta gtgagcatag cgctcgtgag
     5221 agttcaaatc tctccttcgg cataaacgca accaaaatgg ttgcgttttt cttatcaact
     5281 ggcttcgttc tcagaagggg aaaagccctg atggtacaat tgttgcaacg gattcgacaa
     5341 tatgaagagt ttttgggaaa agataaagca gattaactgg gcggacaagc gcgtcttcct
     5401 gattgcggcg gttgtcctgc tcgtattcct gatgatggat tttaacaacc gcatggtccg
     5461 ttcacttcag ctggaaaagc aagagcagca gctgaacgcg cgcatcgccg cgctggagca
     5521 gaccaaacag cgcctggaag cagatatcgc ctatgccaat tccgaccgcg cggtggaaga
     5581 atgggcgcgt gaagaagcca agctgataaa agaaggcgac gttccgatta tcatccgggc
     5641 ccaatccagc gctgccgccg cggccccaac acctgcgccc caggcggaaa accctgagct
     5701 ttcgcgcctg gagatatgga aacagctctt ctggggagag taaacagctc caccgtttcg
     5761 aaaagacacg ctccgcactt aacacgaagc tttttccgcc cggcggctgg acgcggaaag
     5821 cagggtctgc ttatctgcag ccgcacaaaa aacctccagc gaatcgaagg ctgcggtcca
     5881 caccaatatc cgcaggactg cgcccggatt ttctgaaaca ttgccccccc aacaataaca
     5941 gcataaattg gcgcagcaaa acgaatcctt ccgcagttct cgggtataat tttttggtta
     6001 tgaacctgga tgaattaacc caaaaaatgc acgccctggt cgaatccaag ggttggtacg
     6061 ctgaggattc ggtgaagcag cagacgccgc gcaacctggc gatctcgctc gtcctggaag
     6121 cggcggaagt gcttgagctg tttcagtggc aggcagaatg tgaagaccgg gaagccctgg
     6181 ctggcgaact ggcggataca gcgctttacc tgctgcagtt agccagcgtt tccgggattg
     6241 acctggaagc ggcagtgctc acaaaaatca gccagaacca aacccgcctt tggcctgagc
     6301 atctgggcaa ggagctgaaa gcatgattat cgcggttttc ggaagcgcca gacctgtgga
     6361 aggggacgcg ctctacgagg acgcccgccg actgggagcg ctcctggcac agcaggggca
     6421 taccctgatg accggcggtt attgcggcct gatggaagcg gtctccagag gcccggcgga
     6481 agccggcggc agcacgatcg gcgtgacatg cgctgaaatt gagcggcacc gccccggcag
     6541 cgccaacccc tgggtgcagg tcaacatccg cacccagacg ctgaaccagc gcctggaagt
     6601 gctcacccgc cagcccgacg cgatgattgc cctgcccggc ggaatcggta cactcaccga
     6661 gctcagtctc gcgttgaatt tgtcggtcat ctcagcaaac agcgccaagc ctatagtcct
     6721 gctcggcccg gtctggcgcg cggttcttga gaatttttac atactaaatg acggccttac
     6781 ttcgcaggaa gaccttgcgc gcgtgcgctt ttgcgtgacg cccgaggaag cagccgaatt
     6841 ctttcataac aatacgaaca ggaaataagt aatggaaaag aaactgccca cccgccaaga
     6901 aaatttctcc gaatggtaca accagctcgt tttgcgcgcg gaactggccg actacgcgcc
     6961 ggtgcgcggc tgcatggttg tgcgtccgta tggttgggcg ctctgggaaa acatccagaa
     7021 agcgctggat gaacgcttta aggccaccgg tcatgtcaat gccgccttcc ctatgctcat
     7081 cccgcagtcg ttcatgacca aggaaaaaga gcatgtggag ggtttctcgc cggaactggc
     7141 cgtggtcacc tacggcggcg gcgaaaaact ggaggaacct ctggtggtgc gcccaacctc
     7201 cgaaaccatc atcggtacga tgtatgccaa atggattcag tcctaccggg acctgccggt
     7261 tttgattaac caatgggcaa acgtcgtccg ctgggaaatg cgcaccaaac tcttcctgcg
     7321 cacgctcgag ttctattggc aggaaggcca caccgcccac gccaccgagc aggaagcgct
     7381 ggatgagacg gaacgcatgc tgcaagtcta cgcggatttc gcgcgcaccg aagccgcggt
     7441 gccggtcatc cccgggcgta aaagcgagtc cgaaaaattt gccggcgcgg tcgcgaccta
     7501 ctccattgaa gcgatgatgg gcgataccaa agctctgcaa gctggtacgt cgcacttttt
     7561 gggccagaat tttgccaaag ccttcgatat caagttcctg gacgaaagca atcagctcca
     7621 atactgctgg acgacctcct ggggagtttc cacgcgcttt atcggcgcca ttatcatgac
     7681 ccatggggac gaccagggcc tggtgctgcc tccccgcctg gctcccatcc aggtggtcat
     7741 cgttccgata taccgcaagg atgaggaaaa aagcgcggta atggaagcag tcaacgcggt
     7801 acaggcacaa ctaaaagcgc tgcgcgtcaa agtggacgat cgcgaaggcc tgacccccgg
     7861 cttcaagttt aacgactggg agctgaaagg cgtaccgctt cgcatcgaaa ttggccccaa
     7921 ggacgtgcag aacggcaccg ccgcgctcag ccgacgcgac atccccgggc gggacggaaa
     7981 aaccttcctg ccgatggcaa gcctggaaag ctctgtgccg gacctgctgc agaccatcca
     8041 ggagaacatg ctcaccaaag ccgctgcttt ccgcgacagc cacatcttcg aagtaaacga
     8101 ttacgaaagc ttcaagcagg tggtggcgga ccagggttgg gcctatgcct ggtggagcga
     8161 tgaccgtgaa gccgaagccc gcatcaagga ggaaaccaag gccaccctgc gcgtttacgc
     8221 cagcgaccag aacctcgggg aaggccggtg tttctacagc ggcaggaaaa ccaacaaaaa
     8281 agcttacttc gcgcgttcct attaagccaa gagcatgaca gctatcgacc tgaccaggct
     8341 ccaaaaacaa atcggggacc tggttgccgt gttctccgac ccggcagtgt tcatcaaaac
     8401 cctgcaggag atattgggct tctacgaacg ccgcgcctac cgccccaacc ggcaactggt
     8461 gccgaaaacg tttatgcgct cgtacaattt gcccccgcaa gtcctgcccc tgattgaaat
     8521 cgggctgaag gaacaggccg gtctgcaccc gtcagatgcg ttggcgcttg cggacgcgct
     8581 ttggcagagc ccctattacg aagcgcgcga gctggcagcg gcaatcctgg ggcagctgcc
     8641 ggctgatttt agcgcgcaag tgctgacgcg catagaagag tgggtctctg cgccgctgga
     8701 ccgggcagcc ctgcaaaccc tgctggaaaa agccagcccg ccggcgcgcc tggctggaag
     8761 ctgggagggt ttcatcgaaa agctgctcgg cagtcccatc atccgcatgc agtcggtcgg
     8821 actgctcgcg ctggcaattg acctggagca gcggccgctc agcaagtaca cggggatatt
     8881 caaactggtg cgccccttca ttcaggccgg ggacgaacgc ctggagatga acctgagcct
     8941 ggtagtaaag agcctggccc gccgcacccc gaatgaaagc gcgtatttgc ttaagcaaat
     9001 cctggctgac agctccggca gcgggattga gcgccgaatt cgggagtatt tggcttactt
     9061 cccccccgaa actgccgccg gactgctcga agccgtcaag acccaccaga agattcataa
     9121 agcggactaa ctgtcagtca cccagaaact cttcctggca tagtcagcga agggtttttt
     9181 tgttcgtcat cgcgagccgt ttgcggtaat aaagattgat cgcgcatctg catggcaaaa
     9241 cggcgcggcg atctgcctgt aatatgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnaag accatactcc tcagtatgct tcgccctgcc aggcgctctt ctaggcatca
     9421 gcttgaaaaa ccgttgtcag cctgagccca agcgaagggt tctttgtttg tcatcgcgag
     9481 ccgttttgcc agcgtgtact acagaataat cccgcatctg catggcaaaa cggcgcggcg
     9541 atctgcctgt aatatgcaag accatactcc tcagtatgct tcgccctgcc aggcgctctt
     9601 ctaggcatca gcatgacaag aatagcacta ccgtcatgag cgaatcagct aaacaaaaca
     9661 gcccggtttc ccgggctgtt catctctatc cataacaaac tctaaaatag atggaaaacc
     9721 gcgattatcg gcgcgaaaag cgaagacacc agcgacatca cggtgatgag cgtgttcaga
     9781 gaagggccgg cggtatcctt gaacggatcg ccgacggtat ccccgaccac cgcggccttg
     9841 tgcgcgtccg agccgggacc gccaaacttg ccagtttcaa tatacttctt ggcgttatcc
     9901 cacagcccgc cggcgttcgc catcagcagg gcaaaaatca cgcctgtgaa aatcgctccg
     9961 cccaaaaagc cgcccagcgc gtccggacca agcacaaagc cgataaccag cggcagcccc
    10021 acggccagga aagcaggcag cagcagcgaa gaaagcgcac cccgtgccgc caaaccgaca
    10081 cagcggtcat aatccgggtc ttcagtgcct gccaggatgc cgggtttttc atggaactgc
    10141 cggcgaattt cctcaatcat gtcaaaggcg ttgtgcgtca cggaaagcat cagcagcgcg
    10201 gagaaaagcg gcggagtcat cgcgcccagc agcacaccgg cgataacaat cggttcgcgc
    10261 aggctgatat tcaggtcaat tccgtttgct ttcacgactt ccgcgtacgc ggcgaagagc
    10321 gccaaaaccg tcagcgcggc cgcgctgatg gagaaacctt tggtgatagc cttggcggta
    10381 ttgccggccg agtccaaaac atccgagcgt tcgattactt cctgtcccat cccagccatc
    10441 tccgcgatgc cgcgcgcgtt atccacaatc ggaccgtaag catccgcgga aacaatcatg
    10501 ccgctgatgg aaagcattcc aaccgccgcc acgccaatcc cgtaaatccc tggcaagcca
    10561 tccccaagcg cgacagagta agaaatcaac gtcgcgacga caaccccgat aatcgaagga
    10621 acgattgaaa tcaaaccgta gctgaacccg gtgataatcg tcacggcaga gccggaagtg
    10681 gacacccgcg ccgtttcctt caccggttta tattggtcat tggtaaagta atccgaggtg
    10741 aaaccaataa tgacgcctgt gacaataccg accgcggcgg accagaagat gccgggttca
    10801 atcaccttca gcaggctcag aacgagaagc gcgccgacga aaataatgca cgtgatgatg
    10861 gtgccgcggt tcaaagccgg gccgggtttc ccactttctt tgactttcac aaactgaatg
    10921 ccaagcaaag aggcgaaagt cccgagcgtg cagagaatga gcggaatcac ggtgtatttg
    10981 tactgcatcc cggtcgaaaa gctggcgccg aggagcatca cagccacagc gctggcaaca
    11041 taactgtcga aaatatccgc gcccatgcct gccacatccc ccacgttatc gcccacatta
    11101 tcagccacca ccgccgggtt gcgggggtca tcctcgggga tgccaatctc aaccttgccc
    11161 accaggtccg cggcaatatc cgcggttttg gtgtagatac cgccgccggc tttggcaagc
    11221 agcgccaggg tgctggctcc aaagctgaaa ccgagcactg catgcgcctc ccccgtgagg
    11281 atgaagatga tgctcatgcc aagcacagcc agtccgacca tcgccagccc gagcaccgcg
    11341 ccggcataaa aagaaagccg gaaagcctgc tgcaggctct tctgagccgc ggtcgcggta
    11401 cggacgttcg cctccaccgc cacgcgcatg cccatataac ccgccaccgc agagcacagc
    11461 gcgccgatga taaaacacgc cgccataatc acgccgctgc cgggttctat cgtcacgccg
    11521 gtcgtgccga tcgtttcaaa attgaaacta aagattaacg cgatgataat tcccaggatc
    11581 agcgccaggg tggccagcga ggtatacagg cgcttgaggt aagtattcgc gccgctgcgt
    11641 atccacgagc ctacctgctg ggtccgttcc gagccaggat cctgccgcat aacccaaaag
    11701 tacaaataaa ccgccacagc gatggaaata attccacccg caatcccaac gtacaaccaa
    11761 gtctgataat ccatttgaaa tgctcctctt tcaaagcctg aagatgctct ttacatacta
    11821 gcggaacgga accagcggta attctccgcg cgcagagtct ccgccgaagg catcgggtag
    11881 taacgcaatt tttcctggtt cccaacaaaa aaaccgttct taattgtgcg gtaagggatt
    11941 accggggagt gagtccggtc gaccatcttg gtggacacaa atttagtttt gacatccgaa
    12001 aggcattggc gcaagtggtc cggattgctg tacggcaggt tgttcaacaa acccatctcc
    12061 gggtcgctgg caagctcacc cagctggagt tccgcgaaag tgatggctgg aacggaaatc
    12121 aaagaagtcg ggttattcac aatcaaatcg tagaagctga gcggatccag ccggctgacc
    12181 accagcggtg taacaggtgc aatttcctgg tatagatgca gaccctcatc cccatcagga
    12241 atgatttcgg aagactttaa cgccagggtg cgtccgtcct gtgtcgtgag gtaaaggtcc
    12301 agcagcgcgc tcgggtcaat atgttcgagc acgcggtaaa ccgaaacata aatggacgac
    12361 tttgggcgcc cgtcttcatg cggcacgcag cgcttaagcg cctcatccag gtggaaatac
    12421 gaattctggt agttggggtc cacctcaaag aacattgcct gaccgcgcga ctttttggag
    12481 gagccaaccg cgtaatagct cccgaattct tccgggttca gcattgagac aatcagggcc
    12541 tcgggaagta aagaaaggta aaaatgcgtt tccatgcatg ctccaatctt atgaattgat
    12601 aatttgcatt tggcgcccat accacgccat gataattgta tctccaaacg ccgctttcgc
    12661 cagcctatcc agcatttttc gcttaaataa aagcagcccg cgctctcgcg ggctgcttgt
    12721 tttctgccag cctctaatgg ctgatcagat agttaatcac cgcgtctttt tcctccgcgg
    12781 tgaaactgac accgtgatgg ttgctcagga actggtccca ttccgcgtag gttttgtcga
    12841 actgcagtac ccaatccagg gcgtgatgac cgccgagttt ttcaatcagc aaagcttcca
    12901 cgtccgcttc gcttaaggcc gcactttgcc cggccgggta agcagcgctg gcggattggc
    12961 cggcttcgcc cgcgggatac atgccggaag actggctttg cgcagggctg acctcagtac
    13021 tttcgccgac agagaccgcc ggcaggttaa tcgaactacc ttcttctttc gtgccgcaag
    13081 cgctcagcag aaggacagct gccaacagga tgcttacgag gaggatattt ttgtttttca
    13141 tactcataat cctttcaagt gtgccgatat tgtaccccat actgtcttat ctgcccgccg
    13201 gggatagggc taaagcgcgc cttccacagc aagttctcaa ctgcttccaa gcggcagaac
    13261 agcgacttct gtaaagtatt cgtctgtgag ccagtccgcg ttctccccca ccggtagaca
    13321 cagggcggaa cagaacaagg ggtgtatacc gacagccaat tcggccattc tgagtgccgc
    13381 ccggcgaatt gaaaagtgcg cgttagcgct cgcgcgcagt ttggtaaagt ggaacaactc
    13441 gcgcaagttc atccggcaga gcacgcgccg gttgtacccg ttggggacga cgtaagcggc
    13501 ggcctccggg tgcactccgc tcagcgcttc atacagctcc gcggccgcgt ccatcgcctg
    13561 ccggtaaagg ctttccacgc cggccaggct catcgccctg gggacagcgt aacccaaacg
    13621 ggtggtgagc ggctgggcgg tctgggtcat catgcggtgc cgtttgaact caaaatacgc
    13681 gccctggtcc ataaccagtt cgaagtcata atgcgcgtac tccagctccc gcaaaggagt
    13741 gtcgaaatcg cccagatgcc cgagaatctc ctgtaccagc gcggccttct cctggtctgc
    13801 caagccgcgt acgtgcgcca ggcactccgc atagctcctg ccgctgaagc gcagcagcgc
    13861 cgcagcaagc acctgctcct cgccggcggg ttccgcgccg agcagtcggc accaatccgc
    13921 cccgggcagc acggcaatgc gcccggcttc cgcgcgcaat ccctcccgga tgccaggcag
    13981 gctctcaatc gggttggcat gcttgacgag tgtcggcagt tcctgctccg ccaccgtttt
    14041 gacctcgctg ccaatttggc gcacttcttc caattccgag ctgagcagtt tttttatcgc
    14101 gtattccagc gcgcgcgcgt ttattgtgac gcccacgttc gccatggagg cggccggcag
    14161 gaagaacctg ccgacgtcca ccgctttcac ccgggcgcgg ttttgccagg ctttttcact
    14221 ttcctcgggc aagcgcccgg tggcgctctc gctccaggcg cgcagggcgg tcatcgcctc
    14281 ggcatagccg tgaaagagca gacccatcac gcggcggtac cgttcctcaa agcgcgaccc
    14341 ggcaatttcc ggcgggagta gccaggcttc ggggttccat tcctggtagc gggtagattt
    14401 ttctgtgtaa gaagccaggc ggttgctttc cagcgtttca atcgccaggc gggagatgtt
    14461 ctcgagcgcc agatgcagca cggcatgctc cgccacgctg gagtggccgt acccgacaat
    14521 ccatttctcg ctgaatttcg aagatttttc ggcgtccaat tccgcggcaa tctggtcaaa
    14581 ggcctcaggt gaacgcgagg ttttcgcgaa ggtaaccgcg atggtttccg cgctgaattg
    14641 gctgggacta agccggtaga ttctcctgga aagattcatg ggtcagtatt ctccgggctt
    14701 cctgtttatc ctgctcaatt tgcctgatga ggtcttccac acgctcaaac ttgatttcgc
    14761 tccgcagatg ctcgatgaat tccacggtga gatcctcgtg gtagatatta tcatcaaaat
    14821 ccagcagcag ggtttcgata tttgcccggg tagtattctc gaaagtcggg cgcacgccca
    14881 cactggtcac gcccaaaagt ttctccccat tgaaacaggc gcgcgtggcg tatacaccgt
    14941 actgcggcag gattttctga gggaacacca cctgattcgc ggtcggaaag cccaatttgg
    15001 agcccagatg tttgccttcc accaccttgc ttttcacgaa atacggccgg ccgagcaaac
    15061 gcgcggcttc ccgaacatcc ccggcttcca gggcttcgcg taccctgccg gaagaaaccg
    15121 gaattccgtc caggcaaaaa ggaggcacca catccatggc gattccgttc tcggcgcaca
    15181 agccgctaat ttggcgcagc gtgccggtgc ggtccttacc cagcgcaaag ttttcgctga
    15241 cggtcagacc gcgcaggtca agatgctcca gcagcagcgc aaagaactgc ccggcgctca
    15301 ggtccgcgag cgcccggtca aatctcagcg taaatacaaa atcgaggccg ctttgctcca
    15361 ggagcagctt cttttcagcc tcgcaagcca gatagaacgg tccgcggata ttctgcagca
    15421 ccacccgcgg gtggggttga aaggtaatca ttgcagtctg gctgccctct ttcgcggcgc
    15481 gctctttcaa ccggtccagc agagctagat gcccgcggtg aagtccatca aaattcccga
    15541 tggtaatcca taagggcagc tttccttccg cgggccagtc ttcgtaaaat cgggtcaagg
    15601 tatcctcaat tttccagcca aaaaaataac ccttttggca tcggccaggc aagggttatc
    15661 ttttactcgc cttcaaggca ttacaggaag aacactttgc ggggctgcca ttcattcgtc
    15721 gcgctgtcgt attccaccaa tgccacaagt tccccttcct gactgaccgc gcgcgcccat
    15781 ttgccaggtt ccacaacgtc cactgccgga acgcggtgtc cgtggcgcat ttcatccact
    15841 tgctccaccg tcagttccac cgtataccaa tcgcccagcg cttccgcggc cgggatgagg
    15901 tattggtacc aatcgccgtt cgtgaaggct tcctgtagtt tgcgcaagga aacggcatcc
    15961 cgcaaaccga aactgccgtt ttttgtgcgg cgcaagccgg taagcgtggc accgcagccc
    16021 agtttttcac ccagatcgtg cgccagggag cgcacatagg tgcccgaaga acattggatg
    16081 tcgatcacag cctcgggtgg gtcccaatcg agcaattcca gttcgtgcac ctgaatcatg
    16141 cggggctcga ggtccacttc ttctcctctg cgggcaagtt cataggcttt ttcgcctttg
    16201 atcttaatcg cggagtaagc gggcggtttc tgttcaaatt cgccgataaa gccctgcagc
    16261 gcttcctcaa actcttcata gctgatttcc acgggcgagc ggcgcatcac acggccttcc
    16321 aggtcatacg tatccgtggt gatccccagc tcaataacgg cctggtagcg cttttccgag
    16381 gcagaaacat actcgctcag gcgcacagcc ggcccaagca gcacgacaag cacgccgctg
    16441 gcgcgcgggt cgagcgtgcc agtgtggcct gctctgcgga ttttagtgcc gttccggata
    16501 atctgcacta catcgtgcga ggataaaccg actggtttat ccaccaccaa caccccggag
    16561 acgctgttgg ccaggttctc tctgctttcc atcatttcat cggacatagt aatctcactt
    16621 atttacagaa tatcccggat cagctggctt ccaggagttg tctcgtgcga tgtaaaacct
    16681 tgctgacaac ttcttccaag gagccttcca catccgctcc agcggctgcg gaatgccccc
    16741 ctcccccaaa ttcgaacgcg attgtagata catcaatacc ctctgcagag cgccaactaa
    16801 cttttacccg gtcctgagtc tgttgaataa ataataccac aacacgcagc ccggagacgg
    16861 aagagagcaa gttaatcaag tcagcatcat cattttcagg atagacagcc ttaattctat
    16921 catcaagcgt caggacggtc caaagtaatt tgtcttcgtg ctggattcgg gtgagaccct
    16981 gcccccaata gcgcagctcc gggatggagc gcgaaagcaa ggtctgccgg tacaccagcg
    17041 gcaaattagc gccgcgatcc atcaaatccg cggcggtgcg caaagttcgc gcgtctgtgc
    17101 cggatgtgcg gaagccaatg gtatctccca ggattccagt caaaaggcac tgcgccgcgt
    17161 tggcatccac cgtcaagccc cagcgaggca ggtgttccgc gagcacgagc gcggttgcgg
    17221 gtgcgtccgc ttccaccagg ttgattttgc caaagttcag gttggtctgg tggtggtcaa
    17281 tgttcaggtc aggtattggc agcccctcga ggacgcttcc gaggcgcttc gggtcgctgc
    17341 aatccacagt aatcagggtg tcgaaaccgc tccggattga gcgcgcaacc ctttccgccc
    17401 cgggcagaaa agcgtacttt tcgggcagcc catcctgcaa gaccagctgc acctgcttgc
    17461 cctccgcgag cagaggcagc cccaaaccaa gcagcgagcc caccgcgtcc gcgtccgggc
    17521 gaatatgcgc agcgatcaca accgagcgcg cgcttttcag cagtttctgg atttgaaggt
    17581 caaccgtttc agtcattgct ttcctggtcc tccccttcat cggctgcctc gccaacctgg
    17641 tcgctttcat cgcgcagctg cgccaggagc gtatcaatcc ggtctgccct ttccggggtt
    17701 ggatcccaaa aaaagcgcag tctgggcatc acgcgcaggt cgatttcgtt ggcgagctcg
    17761 aacttaagaa agccgcgcgc gtgattaagc gcctcgagga cctcgcgttc gcggacccgc
    17821 ccttccggtg ccgagacgta aacgttggca aagtccagct cccggtccac cttgacatcg
    17881 gtgatattca cacccgtcag gcgcgggtct tcaattttgt tcagcagcgt cagcgaaagg
    17941 atttccttga cgcggtcatt aatgcgtttc attcgcagtg gtgtaggcat cttccctcct
    18001 gtgaaaggta attagctggc ttagccgcca aatttctcca tgatgtagct ctcgatggta
    18061 tccccaacct caaaatcttc gaagttcttg atcgaaatac cgcattccat cccctcgcgc
    18121 acttcgcgca cgtcttcttt ttcacgcttc agcgaggaaa tttcaccggc aaagacctgc
    18181 tcaccggcgc gcatcacgcg cacgctggcg ttgcggcgga tttcgccatc caaaacccgg
    18241 cagccagccg ccatgccggc tttggatacc ttgaaagtgg ccagcacctg cgcagtaccg
    18301 atgacttttt ccaccatttc cggttcgagc aagcccttaa gcgctttttc gacgtcttcc
    18361 agcatgcggt agatgatttt atacacgcgg atggaaacgc cttcggcttc ggccatatgg
    18421 cgggtgttcg ggtccacatc cacgttaaac ccgaggatga ccgcgttgga agccgccgcc
    18481 agggccacgt ctgactcggt tacattgcct gtttccgaat gcagcacctg aatggaaatg
    18541 tctttttctt ttttccccag gtcttccagg gaatttacga tgggttccag cgagccgcgt
    18601 acgtccgctt tcaaaatcag acgcagttcc cgggtttcgc cggatttcac cttggcgaat
    18661 aattcttcca gtgtggcgcg cttaatattg cgcttggctt cgcgttcagc tttccggctt
    18721 tcgacaatcg cgcgcgcttc gcgttcattt tcgactacct gaaaaaggtc cccggcatcc
    18781 ggcaaatggt ccaggcccat caccacaacc ggcgtggagg gcggcgcttt ttcgatgcgc
    18841 ttgttgcgct ggtcgaacat ggctttaatc ttgccgtagc tttccccggc gacgaccaca
    18901 ctgccgacct tgagggtgcc gttttgcacc agaagggttg ccatcgggcc ggtggttttg
    18961 tccagcttcg cctccacaac ggtgccgatg caggcgccct tggggttcgc tttaataatc
    19021 atattatcgg cgaccagcag gatggtttcc agcaggtcat ccaaaccttt gcgggtcttc
    19081 gctgagacgg gtac
//
LOCUS       DAOQAD010000030        18907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_3047_length_18907_cov_6.179610, whole genome shotgun sequence.
ACCESSION   DAOQAD010000030 DAOQAD010000000
VERSION     DAOQAD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18907
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3047_length_18907_cov_6.179610"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PKL60_03990"
     CDS             <1..791
                     /locus_tag="PKL60_03990"
                     /inference="COORDINATES: protein motif:HMM:NF020952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNW95329.1"
                     /translation="IFAEANVRNITEYNNKQSAAGGKTLPFYLIVIDELADLMLLAPT
                     ETEKLLTRLAQLARATGIHLVLATQRPSVDIITGLIKANFPARIAFAVASMTDSRVII
                     DQPGAESLLSKGDMLFQAPDSPAPVRLQGTFISETELNRLVDFWRNQAQQKTAEERPD
                     IAVFASQSRPPLVDDGQTQPPLWQETSKNSDEDPLTTEVIRIIRHEGRASVSLLQRKL
                     RIGYSRSARIIESLEERGIVGPPNPQTGTREVLDYGEYGPLDGD"
     gene            918..2228
                     /locus_tag="PKL60_03995"
     CDS             918..2228
                     /locus_tag="PKL60_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNW95330.1"
                     /translation="MTDIDDVSKQENKEENEPQTMEQMGDLLESNLSIDFPKAGEIRD
                     GIIASIGENQILVSVGAKSEGIITGKEYEAIPADILATLTVGQAIPVYVVTPEDQSGN
                     LVLSFIRAVEEQSWLDAEEMKRSGQSFESEIVGYNKGGLLVPLSTIRGFVPASQLSLT
                     RRSEISGETPEEKYKAMIGEKVQCCVIEVDRERHRLILSERAASTETRDMVREKILDS
                     LKEGDVRTGRVTSLADFGAFVNIGGADGLVHVSEISYEHITHPSEVLKVGDEVKVKII
                     SVDKERRRIGLSIRQLQNDPWSSQVTALKPGQLVEGEITRLTNFGAFAKLNVPGDMEG
                     LIHISELSEKRVEHPKEILKVGDRVALRILRIEEDTHRIGLSMRRVDSPQYADQDWKS
                     LAQDLEVTSSSEGATLGDIVGEIPQTPAEETPKPEASDSEETQA"
     gene            2283..3386
                     /locus_tag="PKL60_04000"
     CDS             2283..3386
                     /locus_tag="PKL60_04000"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNW95331.1"
                     /translation="MNWFIDAHEDLAWNMACFGRDYSRSAYQTRLSEQGTPFPAYTGD
                     TMLGWPEYNQAKVALVFGTLFAAPARPGHADLLDKQTYNSPREANRIAWNQLGSYHRL
                     CDQKPHAFRLLGTQTELAEHLARWQAKPPQKEGNGFQPVGLIPLMEGAEGILNLDELP
                     RWWEAGLRIIGLAWAGTRFSGGTREPGPLTGEGKALLEAMAELGFILDLSHMDAAAAF
                     QALERYEGTIITSHANASRPVRAYSGNRLLDDDLLRAMIEREVVIGAVPFNNFLQADW
                     AQNGGRQAVSLSMVADQIDYICQIAGDARHVGIGTDFDGGFGLQSSPRELNSIADLPL
                     LLPLLSAKGYSETEQEDIAHGNFLRVLQSALPR"
     gene            3408..3890
                     /locus_tag="PKL60_04005"
     CDS             3408..3890
                     /locus_tag="PKL60_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95332.1"
                     /translation="MEPNCPPNNPQAPAKANGNHNGNRRFRGRSGLTLLIVGFILFIL
                     GAKPEVFGLNRGVSIGFVQIIVILLGLAVLTLGGSLALLSLWGKNQKSLLADFGTRTI
                     ATGYVICFFTALADAFGFGTNPMPNVFLGRLQSQGLIIGMAVIGVGFLMLLPYKPARH
                     "
     gene            3934..5229
                     /locus_tag="PKL60_04010"
     CDS             3934..5229
                     /locus_tag="PKL60_04010"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNW95333.1"
                     /translation="MNEQLITYTLENGLTLLIREIHTAPIVSCWVWYGIGSRNEVPGK
                     TGLSHWVEHMQFKGTAQHSASWMDHTIARLGGHWNAFTSPDWTTYFETLPSSALETAL
                     ALEADRMRGSLFDPAEVETERTVILSEREGLENNPLFLLNEAVQLRAFREHPYRNEVI
                     GAKADLLALTREDLYGHYQNTYQPSNALLCLAGDVDPEQALALTRQYFGDIPSQPVEK
                     HIPAPEEELPSPERVELRGPGDTTFVEIVWRAPKANSDDFFAFTILESLLTGPSSLNM
                     FGGGGISNRTSRLYLALVEKELSVSVNGNLNASIDPGTFIINLTKHPHNSISKLLAAL
                     DKEIDKIQTKPVPQAQIDRAVKQAKALFAYSSDNITNHGFWMGYASSFADYSWFTRYV
                     ERLAAVTPEQVCSTARKYLRPDARVIGVYTPNGEESGGD"
     gene            5222..6511
                     /locus_tag="PKL60_04015"
     CDS             5222..6511
                     /locus_tag="PKL60_04015"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNW95334.1"
                     /translation="MTELLFSTKTLPGPDDITREEFSNGSVLLTRPNMSSPAVAIKGY
                     LPPGSSAEKPEKYGLANFMSSMLTAGTWRHAFRELHDEIESMGASLSIGAGGLATSFS
                     ANCLREDLPRLLALIREVFSEPSFPIRQVRRIKTQLLTFLAIQAQDTAAMADQAFDRA
                     IYGSHPYAIPELGFPHTLQRISREDLEDFQQRYLGPKGMVVAICGGVLPAEALAAFSQ
                     TLGVWVNAYQQPQPELPPVQALHGIIREHVTLEEKSQTDLVLGTFAPKTTGPDYLPAQ
                     LGNEILGQFGMMGRIGESVREKAGLAYYAQSELGSGIGPTTWQVIAGVNPRNLDKAVA
                     LIREELARFTSEPVSEEELADVRSALIGQLPLSMESNAGVAGALLNLERYQHGLNYYR
                     ELPARISSVTREDILRTAQTYWQLENLVIASAGVALS"
     gene            6508..6870
                     /gene="acpS"
                     /locus_tag="PKL60_04020"
     CDS             6508..6870
                     /gene="acpS"
                     /locus_tag="PKL60_04020"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNW95335.1"
                     /translation="MIKCGVDIVKIERLDAITATIRARFIQRVFTPLEQAQLGTESEA
                     LAGVFAAKEAASKALGTGIGRVSWQDIEILHAGSGEPELRLHGEALRAALARGYSEWA
                     VSITHDGGLALAFVVAQG"
     gene            6883..7527
                     /locus_tag="PKL60_04025"
     CDS             6883..7527
                     /locus_tag="PKL60_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95336.1"
                     /translation="MKILALSDVELPAVYSSRIRERFQQTDLVISCGDLPYYYLEYVL
                     SSLDIPLYYVHGNHVTEVAQAGGETRAEPWGGVNLHRKVVYDRKLDLVLAGIEGSVRY
                     SKKAHQYSQARMWWMVLGLVPGLLLNKLLHGRYLDILVTHSAPEGIQDDKDLPHRGIQ
                     AFRWLIRTFKPRLALHGHVHLYTPIIPWKTQFKETLVVNAYGYREIPWPLPENW"
     gene            complement(7535..8821)
                     /locus_tag="PKL60_04030"
     CDS             complement(7535..8821)
                     /locus_tag="PKL60_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNW95337.1"
                     /translation="MPDYSDTILPPLLSRKSPFLSDGNDLVLPDNTGHSLCNVPGTVS
                     KLLNGPELSAPALNQEILDKFEGRYRNVILLLVDALGYDHLQRLMAAGKAGFWKENLN
                     RASLFPLTSICPSTTASALTTLWTGTEPAQHGYIGYEMWLKRYSMVVNTILHMPASYF
                     GDAGSLTKSGFVPESFLGLKPLGVQFAERGIESHAFLPYSIGGSGLSRMHLEKASVHG
                     YVAESDMWANLRDLLNTPARGARFLYVYWSTLDSLIHRYGPDDARVNNQFSDLSRSLE
                     VNLLGGLEKSVREKTLLLLTADHGAIATPVHEKYNLSKHPDFLRMLQMLPTCEARLPF
                     LFIKPGLEAEVRAYFANSWPGQFTLLSIEEALSMRLFGAGEEHPELRDRLGDLIVVSR
                     GDAYLWWPDKPNVMQARHGGLHRLEMLIPLYALPLG"
     gene            complement(8814..9611)
                     /locus_tag="PKL60_04035"
     CDS             complement(8814..9611)
                     /locus_tag="PKL60_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNW95338.1"
                     /translation="MDFSEFPVLDFDPDEEAVISPPKKEIPGGFPRHVVICFFREVIE
                     KVARERSAKKLFSICSEGGEHPIYEIALNGERLAFFFPGVGAPLAAGLMDEVIALGGR
                     AFIGCGGCGVLDRSIAVGHLLVPNSAVRAEGTSYHYLPPSREVQADADALQAIELALQ
                     TRGVAYRLTKTWTTDAFYRETPAKVRKYQAQGCLAVEMEAAALMAVARFRGARFGMLL
                     YGGDLVQAEGWDHRDWHDRAEIRESLFWLAAEACLNLKTNPTGEENA"
     gene            complement(9611..10453)
                     /locus_tag="PKL60_04040"
     CDS             complement(9611..10453)
                     /locus_tag="PKL60_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNW95339.1"
                     /translation="MNRVYLGDNLPILRRLPDASVDLIYIDPPFNTGKVQTRRRLKTV
                     RDEAGDRVGFGENSYRTELLSEQAYSDAFSDYEAFLLPRLREAYRLLNRRGSLYFHID
                     YREVHYCKVMLDGIFGRGNFLNEIIWAYDFGGRAKDRWPAKHDNILYYVKDAGDYVFN
                     REAIDREPYMAPGLVGKEKAARGKLPTDTWWHTIVGTNSREKTGYPTQKPTAILERIL
                     RASSNPADLVLDFFAGSGTTGQACLNLGRRFILIDNNPQAWEVMRTRFAAAADIEWIS
                     EGAV"
     gene            complement(10494..10979)
                     /gene="mscL"
                     /locus_tag="PKL60_04045"
     CDS             complement(10494..10979)
                     /gene="mscL"
                     /locus_tag="PKL60_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNW95340.1"
                     /translation="MKKLLEEFKDFIIKGNALDLAIGVIIGGAYGSVINGIVNNLLMP
                     PLGLLLADVDFTDLYIQLNRKAVELAPHTTLAAAKEQGAVVLAYGSFITTLLNFIIIA
                     FSIFLLVKGIQKLRNGTSKLKKNEAPEAAEPTEKLCPFCQTKIPVKAVRCPHCTSELA
                     E"
     gene            11472..11945
                     /locus_tag="PKL60_04050"
     CDS             11472..11945
                     /locus_tag="PKL60_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95341.1"
                     /translation="MDDNTNNIPQETPEDAAKSIGDEWQQIPSASAEEWSKDIPATPV
                     PPEVPVEADRWGAPQPAATEDPNRWSGELYTPGAEAPQAVPPERPAESVVVDVPPVGT
                     TAQAAAPEKKSFPVWAIILIVIFVLMLCCVCPIAIILGIFSSNWQQFSALLLAFI"
     gene            complement(12237..13073)
                     /gene="rsmA"
                     /locus_tag="PKL60_04055"
     CDS             complement(12237..13073)
                     /gene="rsmA"
                     /locus_tag="PKL60_04055"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNW95342.1"
                     /translation="MTRRYLDAHALLREYQLQPKKSLGQNFLVDPNGLEKVMLAAGIG
                     PNDAVLEIGAGLGSLTVLLAQTAGHVVAVEIDRGLLPPLTQALSAYRNVRIVEGDILK
                     LPLEELIPGEGYLVVANIPYYISSAILRRLLGAETRPERMTLTVQQEVAERICARDGK
                     LSLLALSVQVFGAPKIEARIPAGCFYPVPDVDSAVLSLALYPQPLIALEDMDDFFRLA
                     RAGFSQKRKTLRNALSAGLALPAAEIEKLLRAAGIDPQRRAETLSIPEWKRLTAAWKW
                     EK"
     gene            complement(13070..14461)
                     /locus_tag="PKL60_04060"
     CDS             complement(13070..14461)
                     /locus_tag="PKL60_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HNW95343.1"
                     /translation="MQNKNPRKFALSRLWLPALLILAGVILILAQFYGEVTLLVNGAE
                     RQARALAFTPGQLLRAAGYTLQAEDEVSPARNRLNLTHRTIVLNQAREVFIQTAEGGK
                     TVLSAQTLPANLLAEAGLTWFPGDWLLWEGTPLSPSAPLPSGEDLRLEFVPAKRVVLQ
                     FETGTRTFFTQQTTLGDALKEAGIQLRPEDRLSQPLDVELAAANTVNVRLAREIRVQV
                     GGQQYAGLSAAENVGEALRDLGLPLQNLDYARPDESQPVPANGQIRIVRVSEEILLQT
                     EETAYNNTYADDPNAELDTVSVLVPGQIGLVVTRDRLLLEDGVEKARRTDGPWKASDP
                     TDGVLGRGTKVVLRTETINGETIEYWRKVSVYATSYKPSSQGGGTGTASGIPLTKGII
                     AVTVDWYRGMKLQPVYVPGYGRAIIADTGGGIPGRYWIDLGFDDSNYESWHFWTTLYF
                     LTPVPGYIPVVLP"
     gene            complement(14478..17927)
                     /gene="mfd"
                     /locus_tag="PKL60_04065"
     CDS             complement(14478..17927)
                     /gene="mfd"
                     /locus_tag="PKL60_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNW95344.1"
                     /translation="MVKSADHPLLLYLSGLSAYQDLLSRLSEGQYRDSPRFGMGLPRA
                     SRLALLAALRADLGVPILLLTNRADRALGLFDELNFWLGSERNFYFPEPNPLFYEALP
                     WSESTRRERLNVLSELAKYLLPGTQPDPVPPVVTAPIRAVMTRTIPRREFLRSTQRLS
                     VGDKRDLQATIQSWAGLGYQYSNIVVQSGQFSRRGGILDIWSPSETLPVRIEFFGDEI
                     DTLRQFDPATQRTLAAVQKTVIFPASEAMPASAEKAGIAPGNPGEFHLPEIYQFPSSL
                     MDYLPESALILLDGEEFITAAVNDIEEESNARFTALVEQKELPADFPLPYLTWSEIED
                     SFAHRQVLELGHTNAVWGTPLAAAFEPGPRFAGRLKDFQAHLTNISREGQAWTVVSRQ
                     AARLRQLWMESSPERSPTEADAHFLEGSLSGGWVLQRDGRAVHNLLTDSEIFGWSRPQ
                     VRRRAPVASEAPELVFGDLKPGDWVVHVDHGIGQFVGLLSPTIDGVQREYLTIQYKDN
                     DILYVPIHQADRLSRYIGGEGGPPSISRLGSPEWTQTKQRVRRAVQEVAAELLELYAK
                     RQVAEGYRFNPDSDWQRELEASFPYIETPDQLKAIEQVKADMESPRPMDRLLCGDVGY
                     GKTEVALRAAFKAVADGKQVAVLVPTTVLAQQHYDTFRERLATFPVSVEMLSRFRSQK
                     EQTDIIKRLAKKQVDIVIGTHRLISGDVAFNDLGLVIIDEEQRFGVSHKEHLKRLRNS
                     VDVLTLTATPIPRTLYMALTGVRDISNINSPPEERLPIITHIGPYDPKLVREAVIREL
                     DRGGQVFFVHNRVQSIPAVYNHLQNLVPEATIGIAHGQMDEGQLADVMHAFTSGQFDI
                     LLCTSIIESGLDIPNANTLIIDRADTFGLAQLYQLRGRVGRGAQRAFAYLFRSSRHQP
                     TPEGQERLEVLADNSQLGAGYAIAMRDLEMRGAGDLLGTRQSGYIAAVGFQLYTRLLS
                     EAVRLLRAESGLETEPESGLPWLDSASLTINIELPLSSEIPNSYIPDQELRLTLYRRI
                     ASLRREEDLISTEIEFADRFGEPPQGVKDLLFQMKIKIMGQNAGLESISIINNQLVLS
                     YPPLPKGTTQRGLPEVDPSARAGKNAYWVNLAGLRNESWQEVLVRVLRKLLDSRDSPR
                     AAL"
     gene            complement(17911..18498)
                     /gene="pth"
                     /locus_tag="PKL60_04070"
     CDS             complement(17911..18498)
                     /gene="pth"
                     /locus_tag="PKL60_04070"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNW95345.1"
                     /translation="METTNGITLIAGLGNPGLQYRYTRHNAGFLVAQALADTLDIPLK
                     RVKFRCQIGEGKLEGTPLIIAKPLTYMNDSGSAVSSLLHYFKVPLERLLVIHDDLDLP
                     FGVLRLRPGGGSAGQRGVQSIADKLGTSAFPRLRFGIGRPPGRMDPADYVLEKFSASE
                     QELLRMTLEQAAAAARTFIREGLEKAMNQYNGEVG"
BASE COUNT         3993 a         5712 c         5362 g         3840 t
ORIGIN      
        1 gcatatttgc cgaagccaac gtgcgcaata ttaccgaata taacaacaaa caaagcgcgg
       61 ctggcggaaa aaccctgcct ttctatctga tcgttatcga tgaactggcg gacttgatgc
      121 ttctggcgcc caccgagacg gaaaaactgc tgacccgcct tgcccagctc gcgcgcgcca
      181 cgggcatcca tctggtcctt gccacgcagc gcccctccgt ggatatcatt accgggctga
      241 ttaaagccaa cttccccgcg cgcatcgcct tcgcggtcgc ctcgatgacc gacagccgcg
      301 tgatcatcga ccagcccggc gcggaaagct tgctcagcaa gggcgatatg ctcttccagg
      361 cgcctgattc ccctgcgcca gtgcgcctgc aaggcacctt tatttccgag accgagctta
      421 accgcctggt ggatttttgg agaaaccagg cgcagcagaa aaccgctgag gaacggccgg
      481 atatcgcggt gtttgcctcg cagtcccgcc cgcctctggt agatgatggt cagacacagc
      541 cgcctttgtg gcaggaaacc agcaagaaca gcgacgaaga ccccctgacg acagaagtga
      601 tccgaatcat ccgccatgaa ggcagggctt cggtctccct gctgcagcgc aagctgcgca
      661 tcgggtattc gcgctcggcg cgcatcatcg aaagcctgga agaacgggga atcgtcggcc
      721 cgcccaatcc ccagaccggc acgcgcgaag tgctggatta tggggagtac ggacctttgg
      781 acggggactg aaacgggttt ttccccgcgc ttagggagga aaccagcgcg cgccaggcga
      841 atgcgctgtt aagggttgca aaagtagccg ttgtctacta taattacgcc caaatttaac
      901 gttggagtta tttattcatg actgacattg acgatgtaag caagcaggag aacaaggaag
      961 agaacgaacc tcaaaccatg gaacagatgg gggatttgct cgaatccaac ctcagtattg
     1021 acttccccaa agccggggaa attcgggacg gcatcattgc cagcatcggt gaaaaccaga
     1081 ttctggtgag cgttggcgcc aaatccgaag gtattattac tgggaaagaa tacgaagcca
     1141 tcccggcgga cattcttgcg actctgaccg tcggacaggc tatcccggtc tatgtggtta
     1201 ccccggaaga tcaaagcggt aatttagttc tctccttcat ccgtgctgtg gaagaacaaa
     1261 gctggttaga cgctgaagaa atgaagcgct ccggccaaag cttcgaatcg gaaattgtcg
     1321 gttacaacaa gggcggtttg ctcgtacccc tgagcaccat ccgcggtttc gtgcccgcct
     1381 cacaactgag cctcacccgc aggtctgaaa tctcgggcga gaccccggaa gagaagtaca
     1441 aagccatgat tggcgaaaaa gtgcagtgct gcgtcatcga agtggatcgc gaacgccatc
     1501 gcctcatcct cagcgaacgc gctgccagca ccgaaacgcg cgacatggtc cgcgagaaaa
     1561 tcctggacag ccttaaagaa ggcgacgtcc gcaccggccg tgttaccagc ctggctgatt
     1621 ttggcgcctt cgtcaacatc ggcggcgctg acggtctcgt gcacgtttcc gagatttcct
     1681 acgagcacat cacccaccca agcgaagttc tcaaggtcgg cgatgaagtc aaagtgaaaa
     1741 tcatcagcgt ggacaaagaa cgccgccgca tcggcctttc catccgccaa ctgcagaacg
     1801 acccctggtc cagccaggta accgctctga aacccggcca actggtggaa ggcgaaatca
     1861 cccggctcac caacttcggc gcttttgcca agctgaacgt cccgggcgat atggaaggtc
     1921 tcatccacat atccgaattg agcgaaaagc gcgtggagca ccccaaggaa atcctgaagg
     1981 ttggcgaccg cgtcgcgctg cgcatcctgc gcatcgagga agacacccac cgcatcggtc
     2041 tcagcatgcg ccgcgtggat tctccccagt atgccgacca ggactggaaa tccctggctc
     2101 aggacctgga ggttaccagc tcatccgagg gtgcgaccct tggcgatatc gtcggtgaaa
     2161 tcccgcaaac tcctgctgaa gagactccca aacctgaagc ctccgacagc gaagaaaccc
     2221 aggcttaatt tcccgaaccc acggcgccgg agtacctcct attccggcgc tttttctttt
     2281 ctatgaactg gtttattgac gctcacgaag atttagcctg gaacatggct tgttttggca
     2341 gggactattc gcgctctgcc taccaaaccc gcctttccga acaaggcaca cccttccccg
     2401 cgtacaccgg ggatacgatg ttgggttggc cggaatacaa ccaggctaag gtagcgctgg
     2461 tatttggcac gctctttgcc gcccccgccc gccccgggca cgctgacctg ctggacaagc
     2521 aaacctacaa ctccccgcgc gaagcgaacc ggattgcctg gaaccagctc ggcagctatc
     2581 atcgtctgtg cgaccaaaaa ccgcatgcct tccgcttgct tgggacccaa accgaactgg
     2641 ctgagcacct ggcgcgctgg caggcaaaac cgccccaaaa agagggcaac ggctttcagc
     2701 ctgtcgggct tatcccgctg atggaaggcg cggaaggcat cctgaacctg gacgaactgc
     2761 cgcgttggtg ggaggccggt ttgcgtatca tcggcctggc ctgggcgggc acgcgctttt
     2821 ccggcggcac gcgtgaaccc ggtccgctga ccggcgaagg caaagccctg ctcgaagcca
     2881 tggcggagct tggtttcatc ctggaccttt cgcacatgga tgcagctgcc gcctttcagg
     2941 cgctcgaacg ttacgagggg accataataa ccagccacgc caacgccagc cgcccggtgc
     3001 gggcatacag cggcaatcgt ctgctggacg acgaccttct tcgcgccatg attgagcggg
     3061 aagtcgtcat cggcgccgtg cccttcaaca atttcctgca agcggactgg gcgcaaaacg
     3121 gtgggcggca ggcggtcagc ctgagcatgg tcgccgacca aatcgattac atctgccaga
     3181 ttgcgggcga tgccaggcac gtgggcatcg gcacggactt tgacggcggc ttcggcttgc
     3241 agtcctcccc aagagagctg aattccatcg cggacctgcc tttgctgctg ccgctcctga
     3301 gcgccaaagg ctactcggaa accgaacagg aagatatcgc gcacgggaac tttttgcgtg
     3361 tgttacaatc tgctttaccg cgataatgat tgaaaggccg acaaaaattg gaaccaaact
     3421 gcccaccaaa caacccgcaa gccccagcca aagccaatgg aaatcataat gggaatcggc
     3481 gctttcgcgg acgctccgga ctcacgctgc taattgtcgg ctttatccta ttcatccttg
     3541 gcgcgaaacc cgaggttttc ggtcttaacc gcggcgtttc catcggtttt gtgcaaatca
     3601 tcgtgattct gctgggtctc gccgtgctca ccttgggcgg ttcactggcg ctgctttccc
     3661 tatgggggaa aaaccaaaaa tccctcctgg cggatttcgg cacgcgcaca atcgccaccg
     3721 gttatgtcat ctgctttttc acagcgctgg cggacgcgtt tggctttggc accaacccca
     3781 tgccgaacgt ttttctcggc cggctgcaat cccagggctt aatcatcggt atggccgtca
     3841 ttggcgtcgg gttcctgatg ctgctgccct acaagcccgc ccggcattga gacttttcgg
     3901 ttatcctcat ccgctccctc ccgcattcca cctatgaacg aacaacttat cacctacacg
     3961 ctcgaaaacg gcctgacgct tctgatccgt gaaattcaca ccgcgccaat tgttagctgc
     4021 tgggtttggt acggcattgg ctcccgcaac gaagtccccg gcaaaacagg cctaagccac
     4081 tgggtggagc acatgcagtt caaaggcaca gcccagcatt ctgcctcctg gatggaccac
     4141 accattgctc gcctgggcgg gcactggaat gccttcacca gcccggattg gaccacctat
     4201 tttgagacgc tgccctccag cgcgctggaa actgccctgg cgctcgaagc tgaccgcatg
     4261 cgcggcagtt tgttcgaccc cgctgaggtg gagaccgaac gaaccgtaat cctttcagaa
     4321 cgggaaggac tggaaaacaa cccgctcttt ctactgaacg aagccgtgca gctgcgcgca
     4381 ttccgggagc acccctaccg caatgaggtc atcggcgcga aagccgacct gctcgcgctc
     4441 acccgcgagg acctctacgg gcactaccag aatacctacc agccctccaa cgcgcttctt
     4501 tgcctggcgg gagatgttga ccccgagcag gcgctggcgc ttacccggca gtattttggt
     4561 gatatcccct cccagcccgt ggaaaaacat atacccgcgc cggaagaaga gctgccctct
     4621 cctgagcggg tagagctgcg cggtcccgga gacacaacct tcgtcgaaat tgtctggcgc
     4681 gcccccaaag caaactccga cgatttcttt gccttcacca ttctggaaag cttgctgacc
     4741 ggcccttcca gcctgaacat gtttggcggc ggcggcatca gcaaccggac cagccggctc
     4801 tacctcgcgc tggtggaaaa agagctttcc gtaagcgtga acggaaacct gaacgccagc
     4861 atcgaccccg gtacctttat tattaacctc accaaacatc ctcacaacag catcagcaag
     4921 ctgctggccg cgctcgataa ggaaatcgat aaaatccaga ccaaacccgt tccacaagcg
     4981 cagattgacc gcgcggtgaa gcaagccaaa gccctgtttg cctacagcag cgacaatatc
     5041 accaatcacg gcttttggat gggctatgcc agcagttttg ctgattattc ctggttcacg
     5101 cgctacgttg agcgccttgc cgcggtgacg cccgaacagg tgtgcagcac cgcccgcaaa
     5161 tacctgcgcc cggacgcgcg cgtgattggc gtctatacac ccaacggaga ggagtccggc
     5221 ggtgactgaa ctgttattct cgaccaaaac attgcctggt ccggacgaca tcacccgtga
     5281 agaattttcc aacggttcgg ttttgctcac acgcccgaac atgagcagcc ccgcggtggc
     5341 aatcaaaggt tatctgcccc cgggctcctc ggccgaaaag ccggaaaagt acggcctcgc
     5401 caactttatg tccagcatgc ttactgccgg cacgtggaga cacgctttcc gggaactcca
     5461 cgacgaaatt gagtcgatgg gcgcatcgtt gagcatcggc gcgggcgggc tggccacttc
     5521 tttctccgcg aattgcctgc gggaggattt gccgcgtttg cttgcgctca tccgggaagt
     5581 cttttccgaa cccagctttc ccatccgcca ggtccggcgc atcaaaacgc aactgctgac
     5641 cttcctggct atccaggcgc aggacacagc cgccatggcg gaccaggctt tcgaccgcgc
     5701 gatttacggc tcccatccct atgccatccc tgagctgggc tttccccata ccttgcagcg
     5761 gataagccgc gaggatttgg aggactttca acagcgctac ctggggccca aaggcatggt
     5821 ggtcgccatc tgcggcggcg tcctgcccgc tgaagccctc gccgccttca gtcaaaccct
     5881 gggcgtctgg gtgaacgcat accagcagcc ccagccggaa ctgccccccg tgcaagccct
     5941 gcacggcatc atccgcgagc acgttacgct ggaggaaaaa agccagaccg acctggtctt
     6001 aggaaccttc gcgccaaaaa ccaccggccc ggactacctg ccggcgcagt tgggcaacga
     6061 aatcctgggt cagttcggaa tgatgggacg catcggggaa agcgtgcggg aaaaagcagg
     6121 cctcgcttac tacgcgcaat ccgaactcgg cagcggtatt ggaccaacca cctggcaggt
     6181 gattgccggc gtgaatccca ggaacctgga caaagccgta gctctcatcc gggaagaact
     6241 ggcccgcttc accagcgaac cggtcagcga ggaagagctt gccgatgtgc gctccgcgtt
     6301 gatcgggcag ctgccgctct cgatggaatc caacgccggc gtcgcaggcg cgctgctcaa
     6361 cctggaacgc tatcagcacg ggctcaacta ctaccgcgag ctgcccgcca gaatatcttc
     6421 cgtcacgcgt gaagacatct tacgaactgc ccaaacctat tggcagctgg aaaacctggt
     6481 tatcgcttcc gcgggggtgg cgctttcatg attaagtgcg gcgtggatat cgttaaaatc
     6541 gaacgtttgg acgcgattac ggcgacgatc cgcgcgcgct tcatccagcg ggtcttcacg
     6601 cctctggaac aagcccagct tggcacggaa agcgaagccc tggctggcgt attcgccgcc
     6661 aaagaagccg cctccaaagc gctgggaacc ggtattggcc gggtaagctg gcaggatatc
     6721 gaaatactcc acgccggaag cggcgagccg gaactgcgtt tgcatggcga agcgctgcga
     6781 gccgcgctgg cgcgcggtta ctcggagtgg gcagtttcca tcacgcacga cggcgggctg
     6841 gcgctcgcat tcgtcgtcgc tcaaggctga gctgcgctgg ccatgaaaat tctggccctc
     6901 agcgacgtgg aactaccggc ggtctacagc agccgcattc gtgagcgctt tcaacaaacc
     6961 gaccttgtaa tcagctgcgg cgacctcccg tattattatc tggaatatgt gctttcttcc
     7021 ttggatatcc cgctctacta cgtgcacggc aaccatgtca ccgaggtcgc gcaagccggt
     7081 ggtgaaacgc gcgccgagcc ctggggaggc gtgaacctgc accgcaaagt ggtttatgac
     7141 cgcaaactgg atttggtgct ggctgggatt gaaggctcgg tgcggtacag caaaaaagcg
     7201 catcaatact ctcaggcgcg catgtggtgg atggtattgg ggctggttcc cgggctgctg
     7261 ctcaacaaac tgctgcatgg gcgatatctg gacatactcg ttacgcacag cgcgccggag
     7321 gggattcagg atgataaaga cctgccgcac cgcgggatcc aggcgtttcg ctggttgata
     7381 agaacattca agccgcgctt agccctacac gggcacgtcc acctctacac ccccatcatt
     7441 ccatggaaaa cccagttcaa ggaaaccttg gtcgtcaacg cctacggcta ccgggaaatc
     7501 ccctggcctt tacccgaaaa ctggtaaacc ttcctcagcc cagcggcaga gcgtaaagcg
     7561 gaattaacat ttccaggcgg tgcaggccgc cgtgcctggc ctgcatcacg ttgggcttat
     7621 ccggccacca caggtaggca tccccgcgcg aaacaacgat caggtcgccc agacggtcgc
     7681 gcagttccgg gtgctcctcg ccagctccga acaagcgcat ggagagggct tcttctatgc
     7741 tgagcagggt aaactggccc ggccaggagt tcgcgaaata tgcgcgcact tccgcttcca
     7801 atcccggttt gatgaacagg aaaggcagcc gcgcttcaca ggtcggcagc atctgcagca
     7861 tgcgcaggaa atccgggtgt ttgctcagat tgtatttttc gtggacaggt gtcgcgattg
     7921 cgccgtgatc ggcagttaaa agcagcaggg ttttttcccg gacgctcttt tcaagcccgc
     7981 ccagcaagtt gacctccagg gagcggctca aatcggagaa ttgattattc acccgcgcat
     8041 cgtcaggtcc gtagcggtgg atgaggctgt ccagcgtgct ccagtaaaca tacagaaaac
     8101 gcgcgccgcg cgcgggggta ttcaggagat cgcgcaggtt cgcccacatg tcgctctcag
     8161 ctacgtagcc gtgcacgctg gccttttcca ggtgcatgcg tgaaagcccg gagccaccga
     8221 tggaataagg caaaaaagcg tggctttcaa tccctctctc agcaaattgc acacccagcg
     8281 gcttgagccc caaaaaactt tccgggacga acccgctttt tgttaagctg ccggcatccc
     8341 cgaagtacga cgccggcatg tgcaggatgg tgttcactac catactgtag cgcttcagcc
     8401 acatttcgta gccgatgtag ccgtgctgtg ccggctccgt gccggtccac aaggtggtga
     8461 gcgcgctggc agttgtgctg gggcatatgc tcgtgagggg gaacaggctg gcgcggttca
     8521 ggttttcttt ccaaaacccg gctttcccgg ctgccatcaa gcgctgcagg tggtcgtagc
     8581 ccagggcgtc caccagcagc aatatcacat tgcggtagcg cccctcaaac ttatccagga
     8641 tttcctgatt gagcgcaggc gcgctcagtt caggtccgtt caacagtttg ctgaccgtcc
     8701 cgggcacgtt gcaaagggaa tgccccgtgt tgtcaggcaa aacaagatcg ttcccgtcag
     8761 aaaggaaggg gctcttgcgg gaaagcagag gcggcaggat agtatcgctg taatcaggca
     8821 ttttcttctc cggtcgggtt agttttcaag tttaggcagg cttccgcggc cagccaaaaa
     8881 agggattcgc gtatttcagc gcggtcatgc cagtcgcggt ggtcccagcc ttccgcctgc
     8941 accaggtcgc cgccgtagag cagcatgcca aagcgcgctc cgcggaagcg ggcaacggcc
     9001 attaaagctg ccgcttccat ttccaccgcc aggcagcctt gcgcctggta tttacggact
     9061 ttcgccggcg tttctctgta gaatgcatcg gtggtccagg tcttggtgag tcggtaagca
     9121 accccgcgcg tttgcagcgc caactcaatc gcctgcagcg cgtccgcgtc tgcctgcact
     9181 tccctggagg gcggcaggta gtgatacgaa gtgccctcag cgcgcaccgc cgagttgggc
     9241 accagcagat gccctaccgc gatggaccta tccagcacgc cgcagcctcc gcagccaata
     9301 aaggctctcc cgcccaaggc gatgacctca tccatcaggc cggctgccag cggcgccccc
     9361 acacccggga agaagaaagc caggcgttcc ccattcaggg caatttcgta gatcgggtgt
     9421 tcaccgccct cggaacagat gctgaaaagc tttttagcgc ttcgttcacg ggcaaccttc
     9481 tcaatcactt cccggaagaa gcagatgact acatgccggg gaaagccgcc cgggatttcc
     9541 tttttgggcg gagagatgac cgcttcttca tcggggtcaa aatcgaggac ggggaactcg
     9601 ctaaagtcca tcacacagcg ccttcagaaa tccattcaat atccgcagca gctgcgaagc
     9661 gcgtgcgcat gacttcccag gcctgcgggt tgttgtcaat caggatgaag cgcctgccca
     9721 ggttcaggca agcctgccct gtggtcccgc tgccggcgaa gaaatcgagc accaaatccg
     9781 caggattgga tgaggcgcgc aaaatacgtt ccagaatcgc ggtgggcttt tgggtcgggt
     9841 aaccggtctt ttcgcggctg ttggtgccca cgatggtatg ccaccaggtg tcggttggca
     9901 gttttccgcg ggctgccttt tccttcccga ccaagccggg cgccatatag ggctcacggt
     9961 cgattgcctc gcggttaaag acataatccc cggcatcctt cacgtaatag aggatgttgt
    10021 catgcttagc cggccacctg tctttcgcgc ggccgccaaa atcgtaagcc cagataattt
    10081 cattcaggaa gtttccccgc ccgaaaatgc cgtcgagcat gaccttgcag taatgcactt
    10141 cgcggtagtc aatgtggaaa tacagactgc cgcgccggtt gagcaggcgg taagcttcgc
    10201 gcaggcgcgg gagtaaaaag gcctcataat cgctgaaagc gtctgagtaa gcctgctccg
    10261 agagcaattc ggtgcggtag ctgttctcac caaaacccac ccggtctccg gcctcgtccc
    10321 gcacggtttt caggcgcctg cgcgtctgaa ccttgccggt gttgaatggc gggtcgatgt
    10381 aaatcaaatc gactgacgca tccggcaggc ggcgcaggat aggcaggtta tcgcccaggt
    10441 aaacacggtt catcacggaa gcctttcttt acgattcagc tcgaggccgg ggattattcc
    10501 gccagttcgg aggtacagtg cggacagcgt acagccttta ccgggatttt cgtctggcag
    10561 aaagggcaca gtttttcggt cggttccgcg gcttcggggg cctcattttt cttcaattta
    10621 gaagtgccgt ttctcagctt ctggatgccc ttgaccagca ggaagatgct gaaagcgatg
    10681 atgatgaaat tcaggagtgt ggtgatgaaa ctcccataag ccaatactac cgcgccctgc
    10741 tccttggcag ctgccagggt ggtgtgcggc gccaattcca ccgccttgcg gttcagctgg
    10801 atgtacaggt cggtaaagtc cacatcggcc agcagcaagc ccagcggggg catgagcaag
    10861 ttgttgacga taccgttgat aaccgaaccg taagcccccc caatgatgac gccaatggcc
    10921 aggtccaggg cgttgccttt tatgatgaaa tctttgaatt cttccagtaa ctttttcaaa
    10981 agttctccca agaaatgttt acaagattat aactgttccc atccccacaa actatgtcaa
    11041 ggctaaaaaa aagaaatatc ctgatgccaa ctatttcaaa atgtccctgt ggagtggatg
    11101 aagtcgtcag gacgagtttt ttctcgaaac tacccccccc tatttcttct tttcgataat
    11161 gtttccccat tgccctaacc ctgaccctgc cccagcggca agcggctcta ttctgcctcg
    11221 ggcagccgcc ggaacggaga ggaagagggg aaagtctgct attccctggt gtggttgggt
    11281 attctcattc ttttgaaaaa aaaattgaag agatcgatta atgatttcct ggttcctaat
    11341 ctcaacagga catttcaatt ttgctatttc aggacaagat tggtttgctt tttcacatcc
    11401 caacaaaatg acagcaacct tgcggtttac tgtataatga gggttatcca gaccagagaa
    11461 aagaggaact catggacgat aacaccaata atatcccgca agaaacgccc gaagatgccg
    11521 cgaaatcaat cggcgatgaa tggcagcaaa ttccttccgc ttccgctgag gaatggagca
    11581 aagatatccc cgccacgcca gtccccccgg aagttccggt tgaggccgac cgctggggcg
    11641 ctccgcagcc cgctgcgacg gaagacccaa accgctggtc cggcgaactt tatacccccg
    11701 gcgctgaagc tccccaggcg gttccacctg agaggccagc ggaatccgtg gttgtggatg
    11761 tccctccggt aggtacaacc gcccaggcag ccgcgccaga aaagaagagc ttcccggtct
    11821 gggcaattat cctgattgtc atttttgtgc tgatgctttg ctgtgtctgc cccatcgcga
    11881 ttatcctggg cattttctcc tcgaactggc agcagttttc agctttgctg ctggcattca
    11941 tctaaacgca atgaactgaa ggggaggctg ccaataactc cccttcgttg aaagaaagcc
    12001 cgccgctgca aagccaagac ttttgcgcgt tgctgtgtgc aatggaagga ggccgtccat
    12061 aaagaacggg tttttctttt ggacagccca aagtgggaga aagtgcctga tttaggcgcc
    12121 gtgttgacag cgcaaatgcg caaacgccca taaaaacagg cacggagtgg gaaaaccaaa
    12181 ggggctgtcc gactttttgg acagcccctt tcttgttccc ggaggagaat accggactat
    12241 ttttcccatt tccaagcagc cgtcagccgt ttccactccg ggatgctgag cgtctccgcg
    12301 cgccgctggg ggtcaatccc agcagcccgg agcagcttct cgatttcagc tgcaggcagc
    12361 gccaaacccg ctgacagcgc gttgcgcagg gtcttgcgct tctgcgagaa gccggcgcgc
    12421 gccagtctga agaagtcatc catatcttcc aacgcgatga gcggctgtgg atagagcgcg
    12481 agagagagca ctgccgaatc cacgtccggc acggggtaga aacagcccgc ggggatgcgc
    12541 gcctctatct ttggggcgcc gaatacttgc acggaaagcg ccagcaagct cagctttccg
    12601 tcccgggcgc aaatcctctc agcgacctcc tgctgcacgg tcaacgtcat gcgctccggg
    12661 cgtgtttccg ctcccaacaa gcggcggagt atcgcggagc tgatgtagta cggaatgttc
    12721 gcgacgacca gatacccttc ccccggaatg agctcctcga gcgggagttt gaggatgtcc
    12781 ccttccacaa tgcgcacatt cctgtaagcg ctcagcgcct gcgtgagcgg cgggagcagg
    12841 ccgcgatcga tttccaccgc gaccacatgc ccggcagttt gcgccagcag gacggtcagg
    12901 ctgcccaggc ccgcgccgat ttccagcacg gcatcgtttg ggccaatccc agccgccagc
    12961 attaccttct ccagcccgtt ggggtccacc agaaagtttt gcccgagcga cttcttgggc
    13021 tgcagctgat actcccgcag cagggcatgc gcgtcgagat aacggcgggt cacggcagta
    13081 ccaccggaat gtagcccgga actggcgtca ggaagtacag cgtggtccag aagtgccagc
    13141 tttcgtaatt gctgtcgtcg aagcccaagt caatccaata cctgcccggg atgccgccgc
    13201 ctgtatcggc gataatagcg cgtccgtaac ccggaacgta gaccggctgc agcttcatcc
    13261 cccgatacca gtcgaccgtc acggcgataa tgcccttagt aagtgggatt ccggaggcag
    13321 taccggtccc gccgccctgg gaactgggtt tatacgaggt agcgtacacg ctgactttgc
    13381 gccagtattc aattgtttcg ccgttgatgg tctccgtgcg caaaaccact ttggtgccac
    13441 gtcccaacac accgtcggtc gggtcgctgg ctttccaggg gccgtccgtc cggcgtgctt
    13501 tctcaacgcc gtcctcgagc agcaggcggt cgcgggtgac caccagtcca atttgccccg
    13561 gcacaagcac cgaaacagta tccagctcag cgttcgggtc gtccgcgtag gtgttgttat
    13621 aggcagtttc ttcggtctgc agcaggattt cttcgctcac gcgcacaatc cggatttgtc
    13681 cgttcgcagg gaccggttgg ctctcatccg gtcgggcgta atccaggttt tgcaggggca
    13741 gccccaggtc gcgcagcgct tcgcccacgt tttcagccgc gctgaggcct gcgtattgct
    13801 gcccgccgac ctgaacgcga atttcccgag ccagccggac gttaaccgtg ttcgcggctg
    13861 ccaattcaac gtccagcggc tgcgaaagcc tatcctccgg acgcagttga atgccggctt
    13921 ccttcagcgc gtcgcccaaa gtcgtttgct gcgtaaagaa agtgcgcgtt ccggtctcaa
    13981 actgcaaaac cacccgtttg gcaggcacaa attccagccg caaatcctca ccggacggaa
    14041 gaggcgcgga ggggctcagc ggagtgcctt cccacaacag ccaatccccg gggaaccagg
    14101 tcagcccggc ctctgccagc aggttcgccg gcagtgtttg cgcggaaagc acagtcttcc
    14161 cgccctcagc agtctgaatg aacacctcgc gcgcctggtt gaggacaatc gttctgtggg
    14221 tcaggttcag cctgttccgg gcgggggaaa cttcatcctc agcctgcagc gtgtatccgg
    14281 cggcgcgcaa aagctgaccg ggagtgaacg ccaaagcgcg cgcctgacgc tcagcgccgt
    14341 ttacgagcag ggtaacttcg ccgtagaact gcgcgagaat caggattacg cctgccagaa
    14401 taagcagtgc gggcagccac aggcgcgaca gcgcgaattt ccgcgggttt ttgttttgca
    14461 tctgttttcg cagtatatca cagcgctgcg cgcggtgaat cccggctgtc cagcagtttt
    14521 cgcagcaccc gcaccaaaac ctcctgccag ctttcattgc gcaggcccgc caggttaacc
    14581 caatacgcgt ttttacccgc ccgcgcgctg gggtccacct caggcaagcc gcgttgggtc
    14641 gtgcctttgg gcagcggcgg gtagctgagc accagctggt tattgattat gctgatggac
    14701 tccaaaccgg cgttttggcc cataatctta atcttcattt ggaacagcag atcctttacg
    14761 ccctgcggag gttcgccaaa gcggtcggcg aattcgattt ccgtggagat aaggtcttcc
    14821 tcacgccgca gcgaagcaat ccggcggtag agcgtcagac gcaattcctg gtcagggatg
    14881 tagctgtttg gaatttcact ggaaagaggc agttcgatat ttatcgtaag gctggctgag
    14941 tccaaccagg gaaggccgct ttcaggctcg gtctccaggc ccgattccgc ccgcagcaag
    15001 cgcaccgcct ccgagagcaa gcgggtgtaa agctgaaagc ccaccgcggc aatatagccg
    15061 gactggcgtg tgccaagcag gtcgcccgcg ccgcgcattt ccaggtcgcg catggcgatg
    15121 gcgtaacccg cgccaagctg ggaattatcc gccaaaacct ccaggcgttc ctggccttcg
    15181 ggcgtgggtt ggtggcgcga gctgcggaac aggtacgcga acgcgcgctg cgcgccgcgc
    15241 cccaccctgc cgcgcagctg gtagagctgc gccagcccaa aggtgtctgc ccggtcaatg
    15301 atcagggtat tcgcgtttgg gatgtccagg ccggactcga tgatggaggt gcacagcagg
    15361 atgtcgaact gacctgaggt aaaggcgtgc atcacgtccg ccagctgtcc ctcatccatc
    15421 tgcccgtgcg cgatgccgat ggtagcttcc ggtacgagat tctgcaggtg gttatacacc
    15481 gccgggatag actgtacgcg gttatgcaca aaaaacacct gcccgccgcg gtccagctca
    15541 cggatgaccg cttcgcgcac cagtttcggg tcgtacggtc cgatgtgggt gataatgggc
    15601 agccgttctt cgggcggtga gttgatgttc gaaatgtcgc gcacaccggt cagcgccatg
    15661 tagagcgtgc gcgggatggg cgtggcggtc agggtaagca catccacgga attgcgcagc
    15721 cgcttgagat gttccttatg cgagacgccg aagcgctgct cctcatcgat gatgaccagt
    15781 cccaaatcgt tgaaggccac atccccggaa atcaggcggt gcgtgccgat gacaatgtcc
    15841 acctgtttct ttgccaggcg tttgataata tcggtctgtt ctttctggct gcggaagcgc
    15901 gaaagcatct ccacgctgac cggaaaggtc gccagccgtt cgcggaaggt atcatagtgc
    15961 tgctgcgcca ggacggtggt cggcaccagg actgccacct gcttgccgtc cgccaccgcc
    16021 ttgaatgccg cccgcagcgc cacttccgtc ttgccgtagc ccacatcgcc gcacagcagc
    16081 cggtccatag ggcgcgggga ttccatatca gctttgacct gttcgatggc tttcagttgg
    16141 tcgggggtct caatatacgg aaagctggct tccagctcac gctgccagtc gctgtccgga
    16201 ttgaagcggt agccttcagc cacctgcctt ttggcgtaca gctccagaag ctcggcggca
    16261 acttcctgaa cagcccgccg tacgcgctgt ttggtttgcg tccactccgg gctgcccagg
    16321 cggctgatgc tcggggggcc gccctccccg ccgatatagc gcgacaggcg gtccgcctga
    16381 tgaatgggga cgtagaggat gtcgttgtcc ttgtactgga tggtcaggta ttcgcgctgc
    16441 acgccgtcga tggtggggct gagcagaccg acgaactgcc cgatgccgtg gtccacatgc
    16501 acgacccaat ccccgggctt caggtcgccg aagaccagtt caggcgcctc ggaggcgacc
    16561 ggtgcgcgcc ggcgtacttg cgggcggctc catccaaaaa tttcgctgtc ggtcagcagg
    16621 ttgtggaccg cgcgtccgtc tcgctgcaaa acccacccgc ctgagagcga gccttccaaa
    16681 aagtgcgcgt ctgcttccgt gggcgagcgc tccgggctgc tttccatcca aagctggcgc
    16741 aggcgggcag cctggcggga aacgaccgtc caggcctgac cctcgcgaga gatattggtc
    16801 aggtgagctt gaaaatcctt cagccgaccc gcgaagcgcg gcccgggttc gaaggcggct
    16861 gccaaaggcg tgccccatac cgcgttggtg tgccccagct ccaacacctg ccgatgcgcg
    16921 aagctgtcct cgatttccga ccaggtgagg taaggcagcg ggaaatccgc tggcagttct
    16981 ttctgttcca caagcgccgt gaaccgcgcg ttagattctt cttcaatgtc gttgacagcg
    17041 gcagtgatga attcctcccc gtccagcaaa atcagcgcgc tctccggcag gtaatccatc
    17101 aggctggagg ggaattggta aatttcaggc agatgaaatt cgcccggatt gcccggcgcg
    17161 atgcccgctt tttcagcgga cgcgggcatt gcctctgaag cagggaaaat cacggtcttt
    17221 tgcactgcgg caagggtgcg ctgggtggcg gggtcgaact ggcgcagagt atcaatctcg
    17281 tccccgaaga attctatgcg caccggcagc gtctcggagg gcgaccagat atccagaatg
    17341 ccgccccggc gcgaaaactg accgctctgc acgacaatat tgctgtactg gtaccccaga
    17401 cccgcccagc tttgaatggt cgcctgcagg tcgcgcttat cacccacgct caggcgctga
    17461 gtgctgcgca ggaattcccg tcgcggaatg gtacgcgtca ttaccgcgcg gatgggcgcc
    17521 gtcaccactg gcggcacggg gtccggctga gtgccgggaa gcaggtactt tgccagttcc
    17581 gagaggacgt tcaaacgttc ccgtctggtc gattctgacc agggcagcgc ttcatagaaa
    17641 agtgggtttg gctccgggaa gtaaaaattc cgctctgagc ccagccagaa gttcaattca
    17701 tcaaagagcc ccaacgcgcg gtctgcgcgg ttggtgagca ggagaatcgg cacgcccaaa
    17761 tcagcacgca gcgccgccag cagcgccagg cgtgacgcgc gcggcaagcc cattccaaaa
    17821 cgcggcgaat cgcggtattg cccctccgac aggcgcgaga gcaaatcctg atacgcgctg
    17881 agaccgctca ggtacaaaag caggggatga tcagccgact tcaccattat attggttcat
    17941 tgccttctca agaccttccc ggataaaggt ccgggcggcg gctgcggctt gctccagagt
    18001 catcctgagc agttcctgct ccgacgcgct gaatttttcc agcacgtaat ccgcggggtc
    18061 cattctgccc gggggtcggc caatgccaaa acgcaggcgc gggaaagcgc tggtgcccaa
    18121 cttatctgcg atagactgca cgccgcgctg cccggcgctg cctccgcccg ggcgcaggcg
    18181 cagcacccca aagggcaagt ccaggtcgtc atggattacc agcaaacgtt ccagcggaac
    18241 tttgaaataa tgcagcaggg aggaaaccgc gctgccagaa tcgttcatat aggtcagcgg
    18301 cttggcaatg atgagcgggg tgccctccag cttaccctca ccaatctggc agcggaactt
    18361 gacgcgcttc agaggaatgt ccaatgtatc cgccagcgcc tgcgcgacca gaaagccggc
    18421 gttgtggcgg gtatagcggt attgcagccc ggggtttccc agacctgcaa tcaatgtaat
    18481 gccgtttgtg gtttccatgc gtgagccatc cattatgtgc aatctttcat tttaacacgg
    18541 aattcttcat accccggccg ggtttaaggt ttgacccaca ggatatccat tgcagcaagt
    18601 tgcgctgatg cagtttgagt atttttcccg ggtcttattg cggcttggaa tgcacccgct
    18661 gccccgacag cctatggaga gcctgaggat gtttccatag gaaatcgcaa attagaaatg
    18721 tcctgattaa gataaggagc caggaaatca tcattcgatt ccttcaagtt ttttttacag
    18781 aggaattatc gtaccctgac acaccaagga atggcagact ttctcctctt acaccccgcc
    18841 cctgcgaacg aaattgattc tattctcctg gtgaacggtc gctgagacgg ggagctgatt
    18901 tttgagg
//
LOCUS       DAOQAD010000031        18755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_3083_length_18755_cov_6.380856, whole genome shotgun sequence.
ACCESSION   DAOQAD010000031 DAOQAD010000000
VERSION     DAOQAD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18755
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3083_length_18755_cov_6.380856"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PKL60_04075"
     CDS             complement(<1..957)
                     /locus_tag="PKL60_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNW95346.1"
                     /translation="MNKHTLRVFFPILLMVGLVFGLVQSTNTAAASSEQGLEKVETAV
                     LDALTAKGSSDYVIEMAEQADLSKAYEMTDWNERGWFVYDTLRETAARTQAPIIAILE
                     KNGVKYQSFFAGNEIAVTGSDLAVLSQIAALDSVGHVRYPRTATIDPLPLLTQNLFDF
                     SIDALDWGITDTNAPAFWSTFGVQGEGIVVANIDTGVQWNHPALDQAFKCASPSDPTC
                     WSDPSNQCGGSACDNNGHGTHTMGTMVGDDDPTLTYQVGMAPNAKWIACKGCETNNCS
                     DSSLNACADWILAPGGSPDNRPNIVNNSWGGGGGDTWYLAKVN"
     gene            1293..1946
                     /locus_tag="PKL60_04080"
     CDS             1293..1946
                     /locus_tag="PKL60_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNW95347.1"
                     /translation="MEIVWYGHSCFRITERGMASVVTDPFDAAVAGYSPLKLKAEIVT
                     VSCDDPVHNYSKAVKGDPLVINGPGEYEVGGVFITGMHTVSDKKETEGPRNTLYIMDF
                     GTLTVAHLGRVSHSPTQSDVESMGSVNIALVPIGDGASLNAAKAAEVISMLEPNIVIP
                     MYYDTPQSKLNLDPLSKFLKEMGITQLETLESYKITNANAFTEEETHVIALDPKIMP"
     gene            1953..2255
                     /locus_tag="PKL60_04085"
     CDS             1953..2255
                     /locus_tag="PKL60_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95348.1"
                     /translation="MSVKILLTWNIKANAEQEYYGFMVGSFLPWINHKELALTDAWVT
                     LYGNEPQIMVGVIMPTFTAARELLKSAEWLDLRDQLLEFVENFTCKIISQKGAFQL"
     gene            2255..3349
                     /gene="mutY"
                     /locus_tag="PKL60_04090"
     CDS             2255..3349
                     /gene="mutY"
                     /locus_tag="PKL60_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNW95349.1"
                     /translation="MSEPAALLLAWYDHNARQLPWRLDITPYRTWVSEIMLQQTRVET
                     VIPYFERFLRRFPDLPALAQASQADVLHAWEGLGYYSRARNLHKCARLLCQQYACALP
                     ADLEALRKLPGIGRYTAGAIASIAFGIPTPALDANIRRVLARLLEIRELPGSPPGEKK
                     LWEAAEHLLSRERPGDHNQALMELGALVCKPKAPDCPRCPLKGECLAYARGSQEGLPA
                     RKVSPAVPHYIVTAAVLRSGSRVLIARRPQDGLLGGLWEFPGGKLEDTDPDLPACLRR
                     EILEELGVEINVGEPLGVYRHAYTHFRITLHAFACGLLAGQNVPESETVRWVRPEELA
                     GFAMGKVDRLIANRLAHFFQSTSCLPGNQP"
     gene            complement(4003..4626)
                     /gene="upp"
                     /locus_tag="PKL60_04095"
     CDS             complement(4003..4626)
                     /gene="upp"
                     /locus_tag="PKL60_04095"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNW95350.1"
                     /translation="MKNVFPSTHPLIAAKLSRLRDVHTEPKKFRELVRELAAMLAYEA
                     TLDLAVLPIEVQTPLTMTHGVALTDRIGLVPVLRAGLGMVEGVWELMPGAEVWHIGLY
                     RDEKTLKPVAYYNRLPVEPTVSVCLILDPMLATGGSAVATVDILKKWGVSRIKFVGLI
                     AAPEGIARLHGAHPDVPIHIAAVDERLNEHGYILPGLGDAGDRQFGT"
     gene            complement(4649..4975)
                     /locus_tag="PKL60_04100"
     CDS             complement(4649..4975)
                     /locus_tag="PKL60_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95351.1"
                     /translation="MFWSFGAYALFMVGAVMGLTRHAAEPSLWFISMGWVLQMTLILL
                     RALGSGRVQPMERAGWQKAALGLALAFVPVLVYFRLKADLMVFSLLLTAAALLWSLAL
                     LRKKAA"
     gene            5271..5762
                     /locus_tag="PKL60_04105"
     CDS             5271..5762
                     /locus_tag="PKL60_04105"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNW95352.1"
                     /translation="MQWIIDGHNLIPHVRGLSLSDLDDEQALIDLLIRFCRARRDRAL
                     VFFDRAQAGRGGEQKHGAVKAVFVPQPASADAAIASYLRKQGARARNDTLVSSDRMVQ
                     ASARAQHLQVLSSPDFAARIAAALSAPSAPTAPEKPLSAEEVRMWEQMFKNRPDSKPK
                     TQE"
     gene            5773..6633
                     /locus_tag="PKL60_04110"
     CDS             5773..6633
                     /locus_tag="PKL60_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNW95353.1"
                     /translation="MRKDEFSAPVKILAVLAHPDDEAFGMGGTLAHYAKKGAQVYLIC
                     ATLGEAGMVDPKLLQDGQTVAGLREQELRCSAEALGLKQVILLGYRDSGMQGSPDNAH
                     PQALAAQKLEDVVAKVVGHMRALQPDIVLTFDPFGGYGHPDHIRAHQAATQAFYQLRA
                     EQPAESARPFARLYYHVMPPGFLRAAIFLLRLRGKDPRKSGANGDIDLVAIAAHRFPT
                     HARVNYADSAAEQARAFACHASQGGGDRSKGLAALVERVFKPRVDRFSQAYPPPEPGF
                     RQRRGLLPDE"
     gene            6762..7715
                     /locus_tag="PKL60_04115"
     CDS             6762..7715
                     /locus_tag="PKL60_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNW95354.1"
                     /translation="MPSIGTYPDEPTYTIKVVQARTGIKPVTLRAWERRYNLLTPMRM
                     PNGYRLYSERDIQLLLWVQRKLDTGMSISQVAGQFQDARARGFWPETIQNIVPEAPKL
                     KPPRPARDYAEMLFGALIAHNESRANAILEEVKKYFDLVTIFQEVMAPSLELVGEAWY
                     NGEIRIATEHLASSYLKGKLLNTMQNLPVVKEGALILVGCGPEETHELAALMFAVLLR
                     QEGYFVEYLGPDLPTDDLLDYAAIVRPKLIVLSVNQETTADLMQGFAAELANLRGKPR
                     LAYAGHYFDQNEQARRDLGGIYLGKTLAEAVEKIKQLITLV"
     gene            complement(7768..10776)
                     /gene="glyS"
                     /locus_tag="PKL60_04120"
     CDS             complement(7768..10776)
                     /gene="glyS"
                     /locus_tag="PKL60_04120"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNW95355.1"
                     /translation="MTDSEHKNPLDFQSIMFALQNYWARQGCLIWQPYYSQVGAGTMN
                     PATFLRVLGPEPWKVAYVEPSVRPDDGRYGINPNRLQMHYQFQVILKPDPGNPQELYL
                     DSLRAIGIDPHQHDIRFVEDNWESPALSAWGLGWEVWLDGQEITQFTYFQQSGGMVLD
                     VPAVEITYGLERIAMALQRVRHFKDIRWNAERTCGDVNQVGEREHSTYYFEVADVPRL
                     REMFNLFESEANLALERDLVLPAYDYVIKCSHTFNILDTRGAVGFTERQALFGKMRDL
                     SRRVAEAYLAQRTEMGFPWLKAVESAPETAQPMLPELKTGTADLLLEIGTEELPTADL
                     AAALAQLETGAQALLADARLEHGEVRIQGTPRRLALMVRDLQTQQPDRVLEVKGPPAA
                     RAFDAQGKPTKAAEGFAKSRGLDVSQLRVSETEGGSYVVAEVSEKGQSAAALLAGRLP
                     ELCESLRFTKGMRWNQTGKVFSRPVRWMAAMLDGEVIPFEFAGLHSGCQTRGLRFAEQ
                     PTCQLNGLQDYLAYMDAQGIVLDPEKRKAQVWEQAQALAAKVGGKLADDPDLLEEVTN
                     LVERPTAMLGTFEQGYLESLPAEVLIGVMRKHQRYFPVLDQDRNLTQHFIIVRNGDAR
                     FEDVVVDGNLQVIHARFADAEFFIREDKQKPLADFIPRLAQLTFQKDLGSMLEKTRRV
                     QSLTTGILNKLGMPADARQAIDRTAELCKADLATAMVIEMTELQGVMGKFYARLSGEP
                     EAVAQGIFEHYLPRNAEDRLPEGEPGLLVGIADRLDSLAGLFAVGLAPTGNKDPFALR
                     RTALGLAQLLTGKNLDFDLEWGLKLAGEALPVPFDSQTLKAVLEFVTGRLRGLLLERG
                     YRYDVVDAVLKAQAANPAKAGRFARSLGAWTAREDWGSILPAYSRCARITRDIKETFL
                     VDENALVEAEERRLLASVRAAQARLEGSPDLDAALEAVRQMVPEINGFFDKVLVMAED
                     EKLRKSRLGLLQSIVALVQPLADLSALEGF"
     assembly_gap    11294..11393
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11628..12386)
                     /gene="recO"
                     /locus_tag="PKL60_04125"
     CDS             complement(11628..12386)
                     /gene="recO"
                     /locus_tag="PKL60_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNW95356.1"
                     /translation="MPDKQDRTFRATAVVLRHIEYGEADRILTLYTLEKGKIQAIAKG
                     VRKIRSRKAGHLEPFTQVALFLAKGRNLAVVTQAEALDSYPGIRADLQRTALAAYVME
                     LLDRFTQEEGENRQLFHLLVATLERLNQDEQPETIVRYYEVQLLDLLGFRPELTHCVV
                     CRREIQPEDQYFSAKLGGALCPRCSAQDSSAWRISMPALKYLRYFQRSPYAVVRGRVI
                     PAAAETELRALMERYFTYLLEYSLRTPGFLDAIS"
     gene            complement(12417..12824)
                     /gene="cdd"
                     /locus_tag="PKL60_04130"
     CDS             complement(12417..12824)
                     /gene="cdd"
                     /locus_tag="PKL60_04130"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNW95357.1"
                     /translation="MLTIQDKENLIRRAKEARELAYAPYSRYRVGAAVLSADGQVFTG
                     SNIENAAYPSSLCAERVAIFKAVSEGHRKLEAVAVVTKNGGSPCGGCRQVMREFGGTQ
                     MLVLIADESGTLLAEFTLDALLPRSFGPDELVP"
     gene            complement(12805..14091)
                     /locus_tag="PKL60_04135"
     CDS             complement(12805..14091)
                     /locus_tag="PKL60_04135"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNW95358.1"
                     /translation="MGSNTLLWAALAAAFALDLLISAAKSTFSHVRLPYLISLREKEP
                     GKVDRTIALIEKVGLRTSLRLGLALAHFLVAGIAVVIAIQNLKIQSLWALLGMIVALM
                     LFVSVFEYLIERRILDAPEKSAIGFTRFASLLDFCFVPATKLMTALLGTHAEKVTLSV
                     TDEALRDWVEVGQPESTLEKGEREMIYSIFHFSETLTREIMVPRIDVLALDVNTTISD
                     ARKEFISAGHSRVPVYEDTIDNVVGLLYAKDLLGIVDGNDTIASQRKLLRPAYFVPEA
                     KKVDELLTELQLRGVHMAVVVDEYGGVAGVVTLEDIMEEIVGEIRDEYDQAEERLYEK
                     TGEGEYVFLGRASIDEFNEVTGLRLPKEHADTLGGLIYSELGRVPQPGEVVNEEGVEF
                     TVEEVFARRIMKVKARLQPEGQAPEEFEGNHVNYPG"
     gene            complement(14081..15439)
                     /gene="glmU"
                     /locus_tag="PKL60_04140"
     CDS             complement(14081..15439)
                     /gene="glmU"
                     /locus_tag="PKL60_04140"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNW95359.1"
                     /translation="MKTASIILAAGQGTRMRSRLAKVLHPLLGKPMLLYALEAARAAA
                     DSAPVLVIGHAAQEVRAAVEAAGADALFALQEQQLGTGHAVLSARGAVPADAQLVLVT
                     CGDMPLLRAETLRQLVNLHEERGGVLTMTSVVGDAPRGFGRILRRADGSVQAIVEEAA
                     ASPEQWSIREYNVSAYCFDAQWLWAHLEKIQLSNKKEYYLTDLVAMAAREGWRVESLV
                     LQDRLEALGINTRVDLADAETALRKRVNESWMLAGVTLLDPATTTIEADVQIGQDTVI
                     LPNTALRGKTIIGEDCRIGPNTTLLHTSVGSGTTICDSVAEYASVGSQVSIGPFCHLR
                     KGAVLADRVHMGNFGEVKSSVLDENVKMGHFSYIGDATIGANVNIGAGTITCNFDGVN
                     KNRTEIGENTFIGSDTMLVAPLKIGKNARTGAGSVVTRDVPDNALVYGVPARERKEQS
                     GQ"
     gene            complement(15540..15613)
                     /locus_tag="PKL60_04145"
     tRNA            complement(15540..15613)
                     /locus_tag="PKL60_04145"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15579..15581),aa:Gln,seq:ttg)
     gene            complement(15792..16211)
                     /gene="tsaA"
                     /locus_tag="PKL60_04150"
     CDS             complement(15792..16211)
                     /gene="tsaA"
                     /locus_tag="PKL60_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNW95360.1"
                     /translation="MNKIPLQPIGVIHTPYQTDGEAPRQAANSRDVGEVVLRPELAEG
                     LEGIEAFPYIILIFAFHLAPAPEKLKVISWRDGVERGVFATRSPHRPNPLGISIVKLL
                     RREGSRLFVEGVDMLDGTPLLDIKPYYSDLDCVEKRI"
     gene            complement(16397..16660)
                     /locus_tag="PKL60_04155"
     CDS             complement(16397..16660)
                     /locus_tag="PKL60_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95361.1"
                     /translation="MKDQPKQFEDDDGRVICDMDVDGMPWHDRRVRRERRQAPKSEHS
                     EQLSSTEIRAYTGSALLAVLLLWAVFAAAWALFILFCTQIWFR"
     gene            complement(16635..17687)
                     /locus_tag="PKL60_04160"
     CDS             complement(16635..17687)
                     /locus_tag="PKL60_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW95362.1"
                     /translation="MEDTNLLLKPRTSSKTSGKLLEVRNLKTHFPIKAGLMQKVVGHV
                     RAVDGISFSIDYGKTLGLVGESGCGKTTVGKTILRLTPKTSGDVIFDGVDVYALDHQE
                     LRAMRPRMQLIFQDPYSSLSPRLPVSEIIGEGVREHGLVENRNLDEYLDHVMRNCGLQ
                     PYHKDRYPHEFSGGQRQRICIARALALNPEFIVCDEPLSALDVSIQAQIINLLMDLQE
                     RLELTYLFISHDLSVVEHISDYVGVMYLGDLVEYADKRTIFGNALHPYTQALLSAAPV
                     PDPTYKMKRIILEGSIPSPANPPSGCKFHTRCRYCMEKCKSVVPVMQEVEAGHMVACH
                     LYNPTNEENERPAETI"
     gene            complement(17690..>18755)
                     /locus_tag="PKL60_04165"
     CDS             complement(17690..>18755)
                     /locus_tag="PKL60_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95363.1"
                     /translation="AIEKQHKRRNVPEYEYLTTMKNPENVVEFDDLHTYFFTDIGTVK
                     AVDGVSFDIPKGKTVGVVGESGCGKSVTSLSLMQLVQRPQGQIVQGAIRFNSGDKVYD
                     IAKTPISVMESIRGSKISMIFQEPMTSLNPVFRAGDQIDEVIALHNPEMNKDEVKNLS
                     LEMIDTVGIANKEGVYNMYPHELSGGMRQRIMIAMALACKPKLIIADEPTTALDVTIQ
                     AQILALLRDLKDKINSSIMLITHDLGVIAEMADFVVVMYAGRVVERGTALEVFKNPCH
                     PYTLGLMASKPVANKQVDRLYSIPGSVPNPINMPNYCYFRDRCNKRFGKCDGLYPEEI
                     FLTETHSVSCYQFDNRELRD"
BASE COUNT         3838 a         5781 c         5178 g         3858 t
ORIGIN      
        1 attcacttta gcgagatacc aggtatcccc accgccgccg ccccaggagt tgttgacgat
       61 gttcgggcgg ttatccgggc tgccgccggg ggccagaatc cagtccgcgc aggcgtttaa
      121 cgagctgtcg gaacagttgt ttgtttcgca gcccttacag gcaatccact tggcattcgg
      181 cgccataccc acctgatagg tcaaggtcgg gtcgtcatcc ccaaccatgg tgcccatggt
      241 gtgggtgccg tgtccgttgt tatcgcaggc ggagccgccg cattggttgg aaggatctga
      301 ccaacaagtc gggtccgatg gggacgcgca cttgaaggct tgatccagcg cagggtggtt
      361 ccactgcacg cctgtgtcga tgttagcaac gacgatgcct tcaccctgca ccccgaaggt
      421 ggaccagaaa gccggcgcat tggtatcggt gatgccccaa tccagcgcgt cgatgctgaa
      481 atcaaagagg ttttgcgtca ggagcggcag ggggtcaatc gtggcagtgc gcgggtagcg
      541 cacatgcccg accgaatcca gcgcggcaat ctggctgagc accgccagat cgctgccggt
      601 cacggcgatt tcattccccg cgaagaatga ttgatacttg acgccattct tttccagaat
      661 ggcgatgatg ggggcttgcg tgcgggcggc ggtttcccgc agcgtgtcat acacaaacca
      721 acctctttca ttccagtccg tcatctcgta agctttgctc aggtccgctt gctcagccat
      781 ctcgatgaca taatcagagc tgcccttggc ggtaagcgcg tccagaacag cagtttccac
      841 tttttccagg ccttgttcgg atgaggctgc agctgtgttc gtagactgaa caagcccgaa
      901 gaccagaccc accatcaaca ggatgggaaa gaataccctt aaagtatgct tattcatctt
      961 tctcctttat agaaaattaa gagttaaaat catgaacgca gaccgtggtc agtattctgg
     1021 agtggacctc cttttccgac gatgccgtca acttagaagg attaagaacg aggacgcagg
     1081 cagataggca attttgatta tggattccag cgcggacagc ctggaaagat tcaacggatt
     1141 gttactatta tagcatccga accgccgctt ggggaataaa atccattcat ttactgagct
     1201 tctactcaag cttggccccg caattcccaa cctgccaagc tgatataatc aaagcgtgga
     1261 ttttttcaca ccctactgtg tttggaggtt acatggaaat cgtctggtat ggtcattcct
     1321 gttttcggat caccgagcgc ggcatggctt ccgtggtgac ggatccgttt gacgcggccg
     1381 tggcaggata tagcccgctc aagctcaagg cagaaatcgt cacagtcagc tgcgatgatc
     1441 cggtacacaa ctacagcaag gctgtcaagg gcgacccgct cgtgattaac ggccccggtg
     1501 aatacgaggt gggcggcgtc tttattaccg gcatgcacac agtttcagat aaaaaagaaa
     1561 ctgaaggacc gcgcaacacg ctctacatca tggactttgg caccctcact gtggcgcacc
     1621 tcggccgtgt ctcgcactcc cccacccagt cggatgtgga atcgatgggc tcggtcaata
     1681 tcgcgctcgt cccgattggc gacggcgcca gcctgaacgc cgccaaagcc gcggaagtca
     1741 tcagcatgct ggagcccaat atcgtcatcc cgatgtatta tgacacaccg cagtccaagc
     1801 taaacctgga ccccttgagc aaattcctga aggaaatggg cattacccag ctcgagaccc
     1861 tggaatccta caaaatcacc aacgccaacg ctttcacgga agaagaaacg cacgttatcg
     1921 cgctcgaccc caaaataatg ccctaggttt ctatgagcgt taaaattctc ctcacctgga
     1981 acatcaaggc caacgccgaa caggagtact acggtttcat ggtcggcagc tttttaccgt
     2041 ggataaacca caaggagctc gccctgaccg acgcctgggt gacgctctat gggaacgagc
     2101 cgcaaatcat ggtcggggtc attatgccca cttttacggc ggcgcgcgag ctgctcaaat
     2161 ccgcggaatg gcttgacctg cgcgaccaac tgctggaatt tgtggaaaac ttcacctgca
     2221 aaatcatcag ccagaaaggc gcgttccaac tttgatgtcc gaacccgcgg cgcttttgct
     2281 cgcatggtac gaccacaacg ccaggcagct gccctggcgt ttggatatca ctccctatcg
     2341 cacctgggtc tctgaaatta tgctccagca aacccgggtg gaaaccgtca tcccgtactt
     2401 cgagcgcttt ttgcggcgct tccccgattt gcccgccctg gcgcaagcct cgcaggcaga
     2461 cgtgctgcac gcctgggaag gcttgggcta ctacagccgc gcgcgcaacc tgcataagtg
     2521 cgcccggctg ctctgccagc agtatgcctg cgcgctgccc gcggacttgg aggcactgcg
     2581 caagctgccc ggcatcgggc gctataccgc cggcgcgatt gcctcgattg cgtttgggat
     2641 ccccaccccg gcgctggacg cgaacatccg ccgcgtgctt gcgcgcttgc tcgaaattcg
     2701 cgaactgcct ggctctccgc ctggcgaaaa gaagttgtgg gaagcagccg aacacttgct
     2761 ctcgcgcgag cgcccgggcg accacaacca ggccttgatg gagctgggcg cgctggtctg
     2821 caaaccgaaa gcgcccgact gtccgcgctg cccactaaaa ggggaatgcc tggcatacgc
     2881 ccgcggcagc caggaaggac tgcccgcgcg caaagtcagc cccgccgtgc cgcattacat
     2941 cgtcaccgcg gcggttcttc gttcgggcag ccgggttctg attgcccggc gcccgcaaga
     3001 tgggttgctg ggtgggctgt gggagttccc gggcggcaag ctggaagata ccgacccgga
     3061 cttgccagcc tgcctgcggc gcgagatttt agaggagttg ggcgttgaaa tcaacgtggg
     3121 cgaaccgctg ggggtttacc gccacgccta cacgcacttc cgcatcacgc tgcatgcgtt
     3181 cgcctgcggg ctgctggccg gtcaaaacgt ccctgagtcg gaaacagtgc gctgggtgag
     3241 accggaagaa ctggccggct ttgccatggg caaggtggac agattgatcg cgaacaggct
     3301 cgcgcacttt tttcaatcta cttcctgtct ccctggcaac cagccttgat gaactgccag
     3361 tactcttcct gccatggaac cgccaccatc agcaaagtag gaaagtaaca gtcagttatt
     3421 ttttgggaac catcgaactg atcgtgatcg ctggggtcgc tttccttggc tcaacagttt
     3481 tccggaataa actcctctgg gatttatcgg cctggtcccg attgtcatgg gggtttttat
     3541 cctcatttga ggagaggacg aaacggagga aacgtgagcc cacggccggc gctggaaaag
     3601 acctttcacg caggcgttct tcatcccttt agccgagggg gtcgacgacc caggtgtttc
     3661 tattccgctc tttatcgccc aaagttggcc agcagccctt cagatgctga acgtgttcct
     3721 ggttgccaag ggacttttct gctgtgcggc gctttccatg gctcctatca ggcctgtgtc
     3781 cgagttcgtt gaacgcttcg agcgctatat cgtctgcgtc gtgttcgtgt tcattggggt
     3841 tatgctgatt ctcgagtctg gccggatacc ctggttaatt tagtaataat tacggtatat
     3901 cgtaatcaat atgacatatt caatagcaat tacgctatat cgtaatcggt tatgacaagg
     3961 aggagaggaa cgctttgttt gctctgcaaa aacggaatag cactacgtcc caaactgcct
     4021 gtcccccgcg tcccccaacc ccggcaggat atacccgtgc tcgttcaggc gctcatccac
     4081 cgccgcgatg tgaatcggca cgtccgggtg cgccccgtgc agccgcgcga tcccttccgg
     4141 cgccgcaatc agcccgacga actttatcct gctcacgccc cacttcttga ggatatccac
     4201 cgtcgcgacg gccgaaccgc cggttgccag catggggtcc aggatcaggc ataccgaaac
     4261 ggttggctcc accggcagtc ggttgtaata cgccaccggt ttcaacgtct tttcatcgcg
     4321 atacagcccg atatgccaga cttccgcccc cggcatcagt tcccacacgc cttccaccat
     4381 ccccagcccc gcccgcagca ccggcaccag cccgattctg tcggtcagcg caaccccatg
     4441 agtcatggtc aggggcgttt gcacctcaat cggcagcaca gccaaatcca gcgtggcttc
     4501 atacgccagc atggcagcca gttcccgcac cagttcgcga aatttcttgg gctcggtgtg
     4561 cacatcgcgc aggcggctga gcttggcggc aatcagcggg tgtgtggaag ggaagacgtt
     4621 tttcatcgca tcatctccaa aagtccaatc aggctgcttt ctttctgagc agcgcaagcg
     4681 accacaatag agccgctgcc gtcagcagca gcgaaaaaac cataaggtcg gccttaaggc
     4741 ggaaataaac caggacgggc acgaacgcca gcgccagccc cagcgccgct ttttgccagc
     4801 cggcgcgttc catgggctgc acccgtccgg agcccagcgc ccgcaggaga atgagggtca
     4861 tctgcagcac ccagcccata ctgataaacc acaaggaagg ctccgccgcg tgccgggtca
     4921 ggcccatcac cgctcctacc ataaataacg cgtacgcgcc aaaagaccag aacatgcctc
     4981 tccctgccgg tgaaatcatc cgcgctaatt atacttccgc gcgcggcgcg ccagagaaaa
     5041 tcagtccggt tttgaacatc ggggggatgc cgccgcggca ttccgcggtc gttttctcca
     5101 tcggaccagt gctcgccaac ctctcgacct acccgctaaa tctgccgcgg agcccaggat
     5161 ggaacagtag cgcgtgctca accgggattg cgcgcgaaag cccctctcaa accttctatg
     5221 ccgccccagc ttgcctgcgc aggcgcctgc caggcggtag aattaggccc atgcagtgga
     5281 ttatcgacgg acacaacctc atcccgcacg tgcgcgggct ttccctctcc gacctggacg
     5341 atgagcaggc cttaatcgac ctgctgatcc gcttttgccg cgccaggcgg gaccgggctt
     5401 tggtgttctt cgaccgcgcc caggccggcc gcggcggcga gcaaaagcac ggcgctgtta
     5461 aggcggtatt cgtcccgcag cccgccagcg ccgacgctgc cattgccagc tacctgcgca
     5521 aacagggtgc gcgcgcccgc aacgacacac tcgtcagttc cgaccggatg gtgcaggctt
     5581 ccgcccgcgc gcagcacttg caggtactct cctctccgga ttttgccgcg cgaattgccg
     5641 ccgcgctcag cgccccctcc gccccaaccg cgcccgagaa acccttgagc gctgaagaag
     5701 tgcgcatgtg ggaacaaatg ttcaaaaacc gccccgactc taaaccgaag acccaggagt
     5761 aaaccaatac ctatgcgaaa agacgaattt agcgcgcctg tgaaaatcct cgcggtactt
     5821 gcccaccccg acgatgaagc ctttggcatg ggcggaaccc tggcgcacta tgccaaaaaa
     5881 ggcgcgcagg tctatttgat ttgcgcgacg ctgggagaag ccggcatggt ggacccgaag
     5941 cttctgcagg atgggcagac cgtcgccgga ttgcgcgaac aggaactgcg ctgctccgcc
     6001 gaagcgctcg gactgaagca ggtaatcttg ctgggctatc gcgattcagg catgcaaggc
     6061 tctccggata acgcccaccc gcaagccctg gcggcccaaa aactggagga cgtcgtcgcg
     6121 aaagtcgtcg ggcacatgcg cgcgctgcag ccggatattg tgctcacttt tgaccccttc
     6181 ggcggctacg gccaccccga ccatatccgc gcgcaccaag cggccacgca agctttctac
     6241 caactgcgcg cggaacaacc cgcggaaagc gcccgcccct ttgcccggct ttattaccac
     6301 gtcatgccgc cgggattttt gcgcgccgcg atttttctgc tgcgcctgcg tgggaaagac
     6361 ccgcgcaaat ccggcgctaa tggggatatc gacctggtcg ccatcgccgc gcaccgcttc
     6421 cccacccacg cgcgcgtaaa ttacgcggac agcgccgcgg agcaagcccg cgcgttcgcc
     6481 tgccacgcca gccagggcgg aggcgatcgc tccaaaggtc tcgccgccct ggtcgagcgc
     6541 gtcttcaaac cgcgcgtgga ccgctttagc caggcttatc ccccgcccga gcccggcttc
     6601 aggcagcggc gcggcttgct gcccgacgaa taggcctgag cgcgggcgga gtaagccgcg
     6661 ggctgacctc caactccgat ttctaataga tttcatatct tctgaacttg ttgctttacg
     6721 ctgcatcttg tacaattggt gtacaaagaa atgagcgcac tatgccatcc atcggaacct
     6781 acccggatga acccacatac acgataaagg ttgtgcaagc ccgtaccggc attaaacccg
     6841 tcacactgcg agcgtgggaa cggcgctaca acctgcttac gccgatgcgt atgcctaacg
     6901 gctaccgcct ctactccgag cgggacattc aactgctgct ctgggtacag cgcaaactgg
     6961 ataccggcat gagcatcagc caggtggcgg ggcaattcca ggacgcccgc gcgcgcggtt
     7021 tttggcctga gacgattcaa aatattgtcc ccgaagcgcc caagctcaaa ccccctcgcc
     7081 cggcgcgcga ctacgccgaa atgctcttcg gcgccctgat tgcccacaac gaatcccgcg
     7141 ccaacgccat tttggaggaa gtcaagaagt atttcgacct ggtcacgatt ttccaggaag
     7201 tgatggcccc gagcctggag ctggtcggtg aggcctggta taacggcgaa atccgcattg
     7261 ccaccgaaca tctcgcttcc agctacctga aaggcaagct gctgaatacg atgcagaatt
     7321 tgccggtcgt caaagaaggc gcgcttattt tggtgggctg cggcccggag gagacgcacg
     7381 aacttgccgc attgatgttc gccgtactgc tgcgccagga aggctacttt gtcgaatacc
     7441 tgggtccgga tttacccaca gacgacttgc tggattacgc cgcgattgtc cgcccaaagc
     7501 tcattgtgct ctcggtaaac caggaaacca ccgctgacct gatgcaaggc ttcgcggcgg
     7561 agctggctaa tctgcgcgga aagcccaggc tggcctacgc cgggcactac tttgatcaga
     7621 acgaacaagc ccggcgcgac ctgggcggta tctaccttgg caagacgctc gctgaagcgg
     7681 tcgaaaaaat aaagcagctg atcaccctgg tgtgatgacc aactgcttat cgtgttttta
     7741 caggcgcccc cttcgggcgt ttgtggttta aaagccttcc agcgcggaaa ggtctgccag
     7801 cggctgcacc agcgccacaa tggattgcag caaacccagc cggcttttgc gcagcttctc
     7861 gtcctctgcc attaccaaca ccttatcaaa gaacccgttg atttccggca ccatttggcg
     7921 tacggcttcc agcgccgcgt ccaaatctgg gctgccttcc aaacgagcct gcgccgcccg
     7981 cacagaggca agcaagcggc gttcttcggc ttccaccagc gcattctcat cgaccaggaa
     8041 cgtctcctta atatcccggg tgatgcgcgc gcagcggctg tacgccggta ggatgctgcc
     8101 ccaatcctcc cgtgcggtcc acgcgcctaa cgaacgggca aagcgcccgg ctttggcggg
     8161 gttggcggcc tgggctttca agacggcatc aacaacgtcg tatcggtatc cgcgctccag
     8221 cagcagcccg cgcaggcgtc cagtcacaaa ttcaagcaca gctttcaagg tctggctgtc
     8281 aaagggcacc ggcagcgctt cccctgccaa cttcaggccc cactccaggt caaaatccag
     8341 gttcttcccg gttagcagct gcgccaggcc cagggcagta cggcgcagcg cgaatgggtc
     8401 cttattcccg gtcggggcca gccccaccgc gaacaagccc gcaagcgaat ccaggcggtc
     8461 ggcgatgccg accagcaggc cgggctctcc ttcaggcaag cggtcttcag cgttgcgcgg
     8521 caggtagtgc tcaaagatgc cctgcgcgac cgcttccggt tccccggaga ggcgggcata
     8581 gaatttgccc atcacgccct gcagctcggt catttcaatc accatagcag tagccaggtc
     8641 agccttgcat agttcagccg tgcggtcgat agcctgacgc gcgtctgccg gcattcccag
     8701 cttattgaga ataccggtcg tgagcgactg gacgcggcgg gtcttttcca gcatggaacc
     8761 gaggtctttc tggaaggtca actgggccag cctgggaatg aaatccgcca gcggcttttg
     8821 cttatcctcg cggataaaga actccgcgtc ggcaaagcgc gcgtggataa cctgcaggtt
     8881 gccgtccacg accacgtcct caaagcgtgc gtcgccgttg cgcacgatga tgaagtgttg
     8941 ggtcaggttc ctgtcctggt ccaacaccgg gaagtagcgc tggtgcttgc gcatcacgcc
     9001 aatcaatact tccgcgggca ggctttccag atagccctgc tcaaaagtac ccagcatggc
     9061 ggtcgggcgc tccaccaggt tcgtaacttc ctccagcagg tcggggtcat ccgccagttt
     9121 tccgccaact ttggcagcca atgcttgcgc ctgctcccag acctgtgcct ttcttttttc
     9181 cgggtccaac acgatgccct gcgcgtccat gtacgccagg taatcctgca gaccgttcag
     9241 ctggcaagtg ggctgctccg cgaagcgcag cccgcgcgtt tggcagccgg agtgcaagcc
     9301 cgcgaattcg aacgggatga cctcgccgtc cagcatggcc gccatccagc gcaccggtcg
     9361 ggaaaagacc ttgccggtct ggttccagcg catgcccttg gtaaagcgga ggctttcgca
     9421 cagctccggc agcctgcctg ccaacagcgc cgcggcggac tgcccttttt cgctgacttc
     9481 agcgactaca tagcttccgc cctctgtttc ggatacgcgc agttggctga cgtccaaacc
     9541 gcggcttttg gcgaaacctt cagcggcttt ggtgggcttg ccctgcgcgt caaaggcgcg
     9601 cgcggcaggc ggtcctttca cttccaaaac gcggtcaggc tgttgggttt gcaagtcccg
     9661 caccatcagc gccaacctgc gcggcgtgcc ctgaatgcgg acttcgccgt gttccaggcg
     9721 ggcatccgcc agcagcgctt gcgcgcctgt ctcgagctgc gccagcgcgg cagccaggtc
     9781 ggcggtgggc agttcttccg tgccgatttc caacagaagg tctgctgttc cggttttcag
     9841 ttcaggcagc atcggctgtg cggtttcagg cgcggactcc acggctttca gccaggggaa
     9901 acccatttcc gtgcgttggg caaggtacgc ttcggctacg cgccgggaaa ggtcgcgcat
     9961 tttgccgaag agcgcctgcc gctcggtaaa accaactgcg ccgcgcgtgt ccagaatgtt
    10021 gaaggtgtgc gagcacttaa tcacgtagtc atacgccggc agaaccaggt cccgctccag
    10081 cgccagattg gcttcgcttt cgaacaggtt gaacatctcg cgcaggcgcg gcacatccgc
    10141 cacttcaaag tagtaggtgg aatgctcgcg ttcccccacc tggttcacgt ccccgcaagt
    10201 gcgctcagcg ttccagcgga tgtccttgaa atgccgcacg cgctgcagcg ccatggcgat
    10261 gcgctccagt ccgtaggtga tttccaccgc gggcacgtcc agcaccatac cgccagactg
    10321 ctgaaagtag gtgaactggg tgatttcctg cccgtccagc cagacttccc agcccagacc
    10381 ccaggcgctt aaagccgggg attcccagtt gtcttccacg aagcggatat cgtgctggtg
    10441 ggggtcgatg ccgatagcgc gcagggaatc caggtaaagc tcctgcggat tgcccgggtc
    10501 gggcttgagg atgacctgaa actggtagtg catttgcagg cgattgggat tgatgccgta
    10561 gcgcccgtca tccgggcgga cggagggctc cacgtaagcc actttccagg gctccggccc
    10621 caaaacgcgc agaaaagtgg ccgggttcat cgtgccggcg ccgacctgcg agtagtacgg
    10681 ctgccagatg aggcagccct gacgcgccca gtagttctgc agcgcgaaca taatcgactg
    10741 aaaatccagc gggtttttgt gttcagagtc agtcataaag attcccttgg tctcggtaat
    10801 ttaacacttg gattatacag caaagcggcc cgcgagcggc gccgcgggat gcctcagaga
    10861 tcagataacg ataccgatag acgaagtctc aagaagaagt aagctttgcg ctattaagag
    10921 accgcgagaa accctgggag gagccaaaga gcaaaaagga agttcgtggg tcgggttgcg
    10981 ttcttccacc cgacaatgct ctcccagcct gaccggcgca ttcgaaggtt cccccatact
    11041 gctttttcgc agggacatga aaagaccccc atccgccctg cgggcgcctt ccccggatgg
    11101 ggaaggtctc aagaccttga ttttacagga tctccggacc atgccaggca ctggaccttt
    11161 aggtctcctc cttgcttttg tcaaacggac aagaccctcc tccgccttgc gggcaccctc
    11221 cccggatggg gaaggtcaca agaccttgat tttacaggat ctccggacca tgccaggcac
    11281 tggaccttta ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctcctcc
    11401 ttgcttttgt caaacggaca agacccccat ccgccctgca ggcgccttcc ccggatgggg
    11461 aaggataaaa ccccctcctc cggcctcatt cttcaacgca ccccggaaca ggcgaaacaa
    11521 cccagcacga aaagttccca gcacaaggtt aacgaacctg gtacatcatc cctccggcag
    11581 catcctatcc gaaacctcga aattctaagc tttattcaca aaaggtttta cgaaatcgcg
    11641 tccagaaagc cgggggtgcg caggctgtac tccagcaagt acgtgaaata gcgctccatc
    11701 agcgcgcgca gctccgtttc cgccgcagca ggaatcacac gcccgcgcac caccgcatac
    11761 ggcgagcgct ggaagtagcg cagatatttc agcgccggca tggaaatccg ccatgctgag
    11821 ctatcttgag cgctgcagcg cgggcacagc gcgccgccca acttagccga aaagtactgg
    11881 tcctcaggtt gtatctccct gcggcagacc acgcagtggg tcagctccgg ccggaacccc
    11941 agcaaatcca gcagctgcac ctcgtaatag cgcacaatcg tttcgggttg ttcatcctgg
    12001 ttcaatcgct cgagcgtggc caccaacaag tgaaaaagct gccggttctc gccttcctcc
    12061 tgggtaaaac ggtccagcag ttccatcacg taagccgcca gggcggtgcg ctgcaagtcc
    12121 gcgcggatgc ccgggtagct atccagcgct tcggcctgcg tgaccaccgc caggttgcgc
    12181 cctttcgcca ggaacagcgc cacctgggta aagggttcca aatgcccggc tttgcgcgag
    12241 cgtatcttgc gcacgccctt ggcaatggcc tgaattttgc ctttttcaag cgtatatagc
    12301 gtcaggatgc ggtcagcttc tccgtactca atgtgccgca gtaccacggc agtcgcccga
    12361 aatgttcggt cctgtttgtc gggcatcccg ctcctgtttg cccccgccaa ctgaacttag
    12421 ggcaccaact catccggccc gaaagaacgc ggcagcagcg cgtccagggt gaactccgcc
    12481 aacagcgtgc cgctctcatc ggcaatgagc accagcatct gcgtgccgcc aaattcgcgc
    12541 atcacctgcc ggcagccgcc gcaaggggag ccgccgtttt tcgttaccac ggcaaccgcc
    12601 tcaagcttac ggtgcccttc cgaaacagcc ttgaatatcg ccacgcgttc tgcgcacagc
    12661 gaggaggggt aagccgcgtt ttcgatgttg cttccggtga acacctgccc atccgcgctg
    12721 agcactgctg ccccaacccg gtagcgggaa tagggcgcgt aagctaattc acgggcttct
    12781 tttgcgcggc ggatgagatt ctctttatcc tggatagtta acatgattgc cctcaaactc
    12841 ctccggcgct tgtccttccg gctgcaggcg cgctttcacc ttcataatgc ggcgcgcgaa
    12901 gacctcttcc acggtgaatt ccacaccctc ttcgttcacc acttcccccg gctgcggcac
    12961 gcgccccagt tcgctgtaaa tcaagccgcc cagggtgtcg gcgtgctcct tgggaaggcg
    13021 cagaccggtc acctcgttga actcgtctat gcttgcccgg ccgagaaata catattcgcc
    13081 ctcgccggtc ttttcgtaca ggcgttcctc tgcctggtca tactcatcgc ggatttctcc
    13141 gacgatttct tccatgatgt cttccagcgt gaccaccccg gccacgccgc cgtactcatc
    13201 cacgacgaca gccatatgca cgcccctaag ctgcagttcg gtcagcagct catccacttt
    13261 tttggcttcc ggcacaaaat acgccgggcg cagcagcttg cgctgcgaag caatcgtatc
    13321 attcccgtcc actatgccca gcaggtcttt ggcgtacagc agacccacca cattgtctat
    13381 ggtgtcttcg tataccggca cgcgcgaatg cccggcgctg ataaattcct tgcgcgcgtc
    13441 gctgatggtg gtgttcacat ccaacgccaa cacgtcgatg cgcgggacca tgatttcgcg
    13501 ggtcagggtc tcgctgaaat ggaagatgga gtaaatcatt tcgcgttcgc ccttttccag
    13561 cgtgctttcc ggctgcccga cttccaccca atcgcgcagg gcttcatccg tgacggagag
    13621 ggtgaccttt tccgcgtgcg tgccaagcag cgcggtcatc agcttggtgg caggcacaaa
    13681 gcaaaaatca agcagcgagg cgaatcgggt aaatcctatc gcggatttct ccggggcgtc
    13741 cagtatccgg cgttcaatca ggtattcaaa gaccgaaacg aacagcatca acgccacaat
    13801 catccccagc agcgcccaga gggattggat tttcaggttc tgaatggcaa tcacaaccgc
    13861 aatgcccgca accaaaaaat gcgccagcgc caggcccagg cgcagcgatg tgcgcaggcc
    13921 caccttttca atcagcgcga tggtccggtc aacctttccc ggctctttct cccgcaggct
    13981 gatgaggtac ggcagcctga cgtgggaaaa agtggatttt gcggcggaaa tcaggaggtc
    14041 aagcgcaaac gccgccgcca aagccgccca taaaagggta ttactgccca ctttgttcct
    14101 ttctctcccg ggcggggacg ccgtatacca gtgcgttatc cggcacatcc cgcgttacta
    14161 ccgagccagc gcccgttctg gcgtttttcc cgattttcaa cggcgcgacg agcatagtgt
    14221 cgcttccgat gaacgtgttt tctccaattt cggtgcggtt cttattgacg ccgtcaaaat
    14281 tgcaggtgat cgtcccggcg ccaatattga cgttcgcgcc gatggtcgcg tccccgatgt
    14341 acgagaagtg ccccattttg acattttcgt ccagaaccga ggacttgacc tcgccaaagt
    14401 tgcccatgtg cacccggtcc gccaaaacag cgcctttgcg cagatggcag aaaggaccaa
    14461 tgctgacttg cgagccaacg ctggcgtatt ccgccaccga atcgcagatg gtcgtaccgc
    14521 tgccgacgct tgtgtgcagg agggtcgtgt tcggcccaat gcggcagtcc tcgccgatga
    14581 tggtcttgcc gcgcagcgcg gtgttgggca gaatgacggt atcctgacca atttgcacat
    14641 ccgcttcaat cgtggtggtc gccgggtcca gcaaggtcac gccggccagc atccaacttt
    14701 cgttcacgcg tttccgcagg gctgtctccg cgtccgccag gtccacgcgc gtgttgattc
    14761 ccagcgcttc cagtctatcc tgcagcacca gggactccac acgccagcct tcccgggcag
    14821 ccatcgccac caggtcggtc aggtaatatt cctttttgtt cgagagttga attttttcca
    14881 ggtgtgccca cagccactgc gcgtcaaagc agtaagccga aacgttgtac tcgcggatcg
    14941 accactgctc cgggctggcg gccgcctcct ccacaatcgc ctgcacgctg ccgtcggcgc
    15001 ggcgcagtat cctgccaaaa ccgcgcggcg cgtcccccac tacgctggtc atggtcaaca
    15061 cgccgccgcg ttcctcgtgt aaattcacaa gctgacggag ggtctcggcg cgcaggagag
    15121 gcatatcccc gcaggtaacc aaaaccaact gcgcgtcagc gggaaccgcc ccccgcgcag
    15181 acagaaccgc gtgcccggtg cccagctgct gctcctgcag agcaaaaaga gcgtccgctc
    15241 cggccgcttc cacagcagcc cggacttcct gcgcggcgtg cccgatgacc agcacaggcg
    15301 cgctatccgc agcagcgcgc gcggcttcca gcgcgtagag cagcatgggt tttccgagca
    15361 gcgggtgcag aactttggcc aggcgcgagc gcatgcgcgt gccctgccct gcggcgagga
    15421 taatggaagc cgttttcatg gtgtactcca aagcgggaac cggcaggcag tgtttttcag
    15481 cggactgctc gccggacaat ctgtatcatc ggggtcgtta tcggtgttca ctgtttttct
    15541 ggctggggca ccaggattcg aacctagatc cacggattca aagtccggtg tgctgccgtt
    15601 gcaccatgcc ccagaattgc gcctatttta acataaatct ttatgcccgc aaacctgcgc
    15661 cggctggcaa aatcaacagt gcagtgcgga tagagatatt atacacactt cagtctgcgc
    15721 attgcccggg gtgagattgt gcacgccgct ctgcgccact gcacttatcg tggaaagccg
    15781 cgccagcgcg ctcaaatccg tttctctaca caatccaggt cggaataata aggcttgata
    15841 tccagcagcg gcgtgccgtc cagcatatcg acaccctcca cgaacaagcg gctgccctcc
    15901 cgccgcagca gcttcacaat cgaaatccct aagggattgg ggcggtgcgg cgagcgcgtg
    15961 gcaaatacgc cgcgctcgac cccatcccgc caggaaatga ccttcagttt ttccggcgcg
    16021 ggagccaggt gaaatgcgaa tatcagaatg atatagggga acgcttcgat tccctccagg
    16081 ccctcagcca gctccgggcg cagaaccacc tcgccgacgt cccgcgaatt tgcggcctgc
    16141 ctgggggcct ctccatcagt ctggtagggg gtatgaatga caccgattgg ctgaagggga
    16201 atcttattca taatgccttc ctcgatcaca atttttttcg tccgcgcaag ttttgcataa
    16261 aagacccggc ggctggctga caaaaaaacc tggagaatct gacaggcaga aatgctgccg
    16321 gattttccag gttcgcatgc ttctcgcagc cagcacagcc agggtcggct tcaggcgatg
    16381 aagcggtccg cgctgctcag cggaaccaaa tctgggtgca gaacaggatg aacagcgccc
    16441 atgcggcggc aaacaccgcc caaagcaaca gcaccgcgag cagggcgctg ccggtgtagg
    16501 cgcgaatttc cgtgctggat aactgctcgg aatgttcgga tttcggagcc tggcgccgct
    16561 cgcggcgcac gcgccggtcg tgccagggca tcccgtccac atccatgtcg cagatcaccc
    16621 gaccgtcatc atcttcaaat tgtttcggct ggtctttcat tttcctcgtt tgtcggatta
    16681 tacaaatggc aagccaccat gtgcccggct tccacttcct gcatcacggg cacaacgctc
    16741 ttgcatttct ccatgcaata gcggcagcgc gtgtgaaact tacagccgga cggaggattg
    16801 gccggggaag gaatgctgcc ctccaggata atccgtttca tcttgtaagt cgggtccggc
    16861 acaggcgcgg cgctgagcag agcctgggtg taggggtgca gcgcgttgcc aaagatggtg
    16921 cgcttatctg cgtattccac caggtcgccc aggtacatga cgccgacgta atcggaaata
    16981 tgctccacaa ccgaaaggtc gtgggagatg aagagataag tgagctcaag cctttcctgc
    17041 aggtccatca gcaggttgat aatttgagct tgaatggaga catccagcgc cgagagcggc
    17101 tcatcacaca cgataaactc ggggttcagc gcgagagctc gcgcgatgca gatgcgttgg
    17161 cgctgaccgc cggagaattc gtgcgggtag cggtctttgt gatacggttg gagtccgcag
    17221 tttcgcatga catggtccag gtattcatca aggttacggt tttccaccaa accatgctcc
    17281 cgaacgcctt caccaataat ttcactgaca ggcaggcgcg gtgagagact ggaataaggg
    17341 tcctggaaga ttagctgcat tcttgggcgc attgcccgca gttcctggtg atcgagggcg
    17401 taaacatcca cgccgtcaaa gatgacatca ccggaagttt tgggggtaag ccgcaggatg
    17461 gtcttcccta cggtggtttt tccgcagcca gattcgccca ccaatccgag ggtcttgccg
    17521 taatcaatcg agaagctgat cccgtcgaca gcgcgcacat gaccgacaac tttttgcatc
    17581 agacccgctt taatcgggaa gtgggtcttg aggttgcgaa cttctaaaag tttcccgctg
    17641 gtcttgctgc tcgttcgggg ctttagtaag agatttgtgt cttccatggt cagtccctaa
    17701 gctccctgtt gtcaaattgg tagcatgaaa cagaatgcgt ctcggtgaga aagatttctt
    17761 caggatacaa tccgtcgcat tttccaaagc gcttgttaca acgatcccgg aaataacagt
    17821 aattgggcat gttaatcggg ttcggtacgc tgccgggaat gctgtaaagc cgatcaacct
    17881 gcttattggc gactggcttg gaagccatca gacccaatgt gtagggatgg cacgggtttt
    17941 tgaacacctc cagcgcggtt ccacgctcaa cgacccgccc cgcatacatg accaccacaa
    18001 aatcagccat ttcagcgatg acgcccaggt cgtgggtaat cagcataatg ctggaattaa
    18061 tcttgtcttt caggtcccgc agcagagcca ggatttgggc ctggatggtg acgtccagcg
    18121 cagtggttgg ttcgtcagcg atgataagct tgggcttaca tgccagcgcc atagcaatca
    18181 taatccgctg gcgcatgccg cctgaaagct cgtgcgggta catgttgtag acgccttcct
    18241 tgttagcgat gccgacagtg tcgatcatct caagcgaaag attcttgacc tcatccttat
    18301 tcatctccgg gttgtgcagg gcgataactt cgtcaatctg atctcccgct ctaaagaccg
    18361 ggttcaggct ggtcatcggc tcctggaaaa tcatggagat tttgctgccg cggatgcttt
    18421 ccatcacgct gataggggtc ttggcaatgt cgtatacctt atcgccagaa ttgaagcgga
    18481 tggcgccctg gacaatctgc ccctgcgggc gttggaccag ctgcatcagc gaaaggctgg
    18541 taacggattt gccgcagccc gattcgccca ccacaccgac ggtcttgcct ttgggaatgt
    18601 cgaaggatac cccgtccacg gctttcaccg tgccgatatc ggtgaagaag tacgtgtgca
    18661 ggtcgtcaaa ctccaccaca ttctcgggat ttttcattgt ggtcagatat tcgtactcgg
    18721 gcacgttgcg gcgtttgtgc tgcttttcaa ttgcg
//
LOCUS       DAOQAD010000032        18140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_3228_length_18140_cov_6.138291, whole genome shotgun sequence.
ACCESSION   DAOQAD010000032 DAOQAD010000000
VERSION     DAOQAD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18140
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3228_length_18140_cov_6.138291"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /gene="hflX"
                     /locus_tag="PKL60_04170"
     CDS             <1..746
                     /gene="hflX"
                     /locus_tag="PKL60_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNW95364.1"
                     /translation="ELEKVRGHRLRHREHRRKTNVPVVALVGYTNAGKSTLLNTLTNA
                     DIYVADQLFATLDPTARQLELPSGQTVVLTDTVGFIQKLPAQLVAAFRATLEEIAEAD
                     LLVHIVDISHPNAALQKKTVLETLLEIGAGDIPIITAYNKVDLLTEEELEDARVNLEN
                     QALLISAKNGSGLDALLDTIGRELLAAYAELDVLLPYDQGQLVSVFHEKGHVQKLEHT
                     EKGICIHGYIPRELQAQFLPYLRKAHKVK"
     gene            complement(791..976)
                     /gene="rpmF"
                     /locus_tag="PKL60_04175"
     CDS             complement(791..976)
                     /gene="rpmF"
                     /locus_tag="PKL60_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNW95365.1"
                     /translation="MTPLPKRKTSSGRRDRRRSHDALKARNSVACPNCGEPRLPHRVC
                     PKCGYYQGREILTIEKD"
     gene            complement(1757..2875)
                     /locus_tag="PKL60_04180"
     CDS             complement(1757..2875)
                     /locus_tag="PKL60_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95366.1"
                     /translation="MTTTEDNPSSGYPREYLRCPYIGLINDVNSYSEFPSDLNYCHHS
                     QPLALPVFIHQRDFCLSSRYSECPMLSSTRYKRLPPEIRLTSASTFKPRLPHWILPVF
                     ILAIFLLGSVATLVIPELSSAFANFLGANPSPSRVYSQTPALTRLGFITPSPYSLFSI
                     IKSFTPSTDEAFTFTDTPAPPSSATLTQTPTANPLCSAEDIKYFSISFFINDVVQIVF
                     DTDKDFSNYQVLDAQGKPTMRLNLPDFKLWKNDTRVYSYGTFMRNAAVLTRLYVEMLA
                     ENKDRVRIAFTDANGTHCSKTIIIPDENAFKTPTSRLPLPTQFIPSPTVELPTLPPTL
                     EPTTEEPPTATPIPPTPTRTPRPSETLPRDTLTPTPNP"
     gene            complement(2967..3446)
                     /locus_tag="PKL60_04185"
     CDS             complement(2967..3446)
                     /locus_tag="PKL60_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95367.1"
                     /translation="MPALLFVCTANRFRSPLAQVMFQEKLRRTADAANWRVESAATWG
                     DPNLPAMPEAVLEANKRQLNLENHRSKLISAQLVKQFDLILVMEAGHKEALQMEFPAV
                     RSRIYLLSEVCGGTPFNILDPYLSGDSPTEVAHEIEDQIEKKFPLLFQWLISNPEKY"
     gene            complement(3463..4371)
                     /locus_tag="PKL60_04190"
     CDS             complement(3463..4371)
                     /locus_tag="PKL60_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNW95368.1"
                     /translation="MNSKEWDSTGLNAGQSGICPYLGLAEDSDSVQDYPSVNNACYHV
                     SPPTTPTLSHQANCCLKAEYARCQVYTAEHNSKMPVELTIKETRPTRKPFRWLFLSLA
                     AATVLFVCLFLLTNGRNSGQTAAPSPTATPTHTAVPFLLIRDATATPTRALALTPTST
                     PVPPTLTPTAQTTRAPHKLDMVIGRQDRFIIHRVSSGESIQLFATQNQTTPDVIKSVN
                     YNLVVPIWVDSIIVIPLDRKDPGDLPAFEPFEIADKGLTLRDIAGQFNVDLDILARYN
                     DLLPEDALEPGEWILIPRERTYWYRY"
     gene            complement(4432..6300)
                     /locus_tag="PKL60_04195"
     CDS             complement(4432..6300)
                     /locus_tag="PKL60_04195"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNW95369.1"
                     /translation="MDIKQIFVAAKRWLWFLVLGTILGAAGGFVFSNRQTPIYQASTR
                     FVVLRAAQTTYDYFSYLDSQQLISTYAQLLSTESLLAQASQELGFPVQKGQATAAQID
                     DTQFVQLTVRDTDPERAALIANGLVKVLIDQNEQLQSVRYISAEQNLQTRIDEAQEQI
                     SILQAQINEISSATVETQLQSVQAQIDDLQNQITGLNTEIAKIDPEQATDAEITLLAD
                     YQAQLDQLTPVLSLYQQIYTNLVVLKTPAQSDANTTTQLDQLRTTLELYQQIYISSIS
                     SLETLRLTRAQNTPNVVQVESAVVPSSPISPKPMQSALLAGGIGFLVTAGIAFLVEFL
                     DDTIKTPDEVKEILHLPVIGLIGELNKKSKNEKLGNYVAYHPRSPVSEAFRSLRTNLE
                     YSSIDKPLRCILVTSAGESEGKTTVTSNLAIVLAQSGKKVLLLDSDMRRPNVHAQFNI
                     HNRVGLSDLVRGKLTLENVVRTLPEVNNLDIITSGGLPPNPAEMLGSERMRTILEELK
                     TLYDIVVLDSPPMLVSDAQILSTRTDGVIYVIVPGLTHSVAAKRPLEELERINANLLG
                     VVMNRIPRNRDYYYGGYNYYSPYSSHQGYHQSTSIEEGFDLGLTDGKASAAPLKRK"
     gene            complement(6570..8555)
                     /locus_tag="PKL60_04200"
     CDS             complement(6570..8555)
                     /locus_tag="PKL60_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNW95370.1"
                     /translation="MKRQLWFSGLFALVLLVSITGERSANAAEALTADRPACTNAEAE
                     LEQVYRVYYQSLEDISKLKSYDLFEFNNNAEKYVLVAASASEITTIQSMGFTVVLDEE
                     QTANYRKLDMLASQGLETLPPPYNCYRTVEELYAYGAALASTYPNLAEWTDVGDSWEK
                     SVGQPDGYDMYVLRLTNESITGDKPKLFITASIHAREYAPAELAARFAGYLIENYQTN
                     ADVHWLLNYHEIHIMITANPDGRKEAESGMSWRKNTNENYCGPTSSNRGADLNRNFSF
                     YWGCCGGSSGSQCAETYRGPSAGSEPETQAVQNYMTAIFPDQRGDPLNMPAAEDATGV
                     FIDLHSYERRVLWSWGFTENPAPNATDLQTLGRKFAFFNNYTPSPSWQLYITDGSTED
                     NIYGRLGVAGYCFEVGDAFFESCNTFEFDILPGNLPALIYAAKTARTPYMTPAGPDSI
                     NLALSQSSVPAGTIVTLTGQVNDTRYKSGSGEPTQPIAEAQYSADLPYWEAGYAPVAL
                     SPADGSFNSSSENVTASINTTGWQPGRHILYVRGKDNLNNWGAVSAIFLTIEGPVNTP
                     PVADGQSLKTRRDLPLNITLTGSDADGDPLSFEVVTNPSRGQLSGALPDLVYTPDPGY
                     IGADSFSFKVWDGLAYSEPAVISIQVEMPLFLPMLAK"
     gene            complement(8565..9836)
                     /locus_tag="PKL60_04205"
     CDS             complement(8565..9836)
                     /locus_tag="PKL60_04205"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNW95371.1"
                     /translation="MAPTPDGNPKSDIPALLLELLNPLSTSETLAAALGGFPSGDWQN
                     FSQYLETHSLANLLYTRLQQNELLDLVPADERRALRENYLANAARNLLLLTRAAEVLA
                     ALQAQGTAVIGLKGLYLLENVYRDIGARAMNDLDILVHREDVPRTIETLRRLSYQAPS
                     YFDSSADNRDVKHVPPMSLADGTTLEVHWTLLEEDEPFSINPQGVWQRARPTRFADAD
                     ALALSTEDLLLHLCIHTSYQHYLKLGLRGLLDIAVVLESEADSIDWRQLLEIAREWGA
                     EKTAALSFALLQEMGWAQIPEAFLSELLPRSLDTDILTKAKALTLQDKRARNAITPDV
                     AALTSTKNLVQKARLILNRIFIPRPALARLYRLNPDSAGIVPGYIRRAFDLFRQYGRT
                     VRKISTMDQAVYQDIEQEKDRFRIHEWLKPE"
     gene            complement(9908..11341)
                     /locus_tag="PKL60_04210"
     CDS             complement(9908..11341)
                     /locus_tag="PKL60_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95372.1"
                     /translation="MPKPIQELKRLIQFMLILLLIVLAGFQTRLASAQPTEPPEEPAQ
                     ERAGIQTGSAGLAWGNPDGPHSVYAWPFTVVQMGHVIQSYQYYGGAPYFHHGIDMLAP
                     QETEVFTRSGGQVVNIENYDYGGLYWEVAILDAEGYVWQYHHIAQPSIPAAIYQAYQA
                     WQADHESGGFIPPNTKLGEIVFWPVVSFGYYFHHIHLNILADGDVYLNPLEFHTLLPD
                     TQTPVIEDLGLTLNNTILTRDTIAEPEILTYGLYAHAGDLFLSPVYTLPPYKIAYSLD
                     GGPWTTLWEFHTFPGGSNDTQLVDDFYISPPTCGDYGCREFYIDLGFTMLGERRFPSQ
                     NGPHTVEVQAWDYNQNSALDTYTWTVTHPSSVVYTGLSAEYTPSGNLIHWSASTLGDA
                     LGFNLYRAEALDGPKTKLNASLIPRGSVNDAQYAFTDASAVLGQVYWYWLEDQDAYGP
                     TGVFYGPQMIKTGVMVYMPILQKIAEN"
     gene            complement(11534..16825)
                     /locus_tag="PKL60_04215"
     CDS             complement(11534..16825)
                     /locus_tag="PKL60_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNW95373.1"
                     /translation="MQKHRLLSTMLIIVCLIVSSFSSLLVSSADAQAETPEQSTSQVF
                     VVRVYYTNQEDIQKLVPFDLFEFNDSVEKYVLVSATQSEIERIQALGFKVTVDEERTA
                     DLALGFGNGIQTIPGYSCYRTVEESFAAAAALAANNPTLAQWIDIGDSWEKSVGQPDG
                     FDMMVLKLTNSQISGTKPKLFIIAGIHAREYTTAETATRFAEYLIANYGTNADITWML
                     DYNEIHILLQTNPDGRKEAETGVSWRKNTNENYCGVTSSNRGADLNRNLSYQWGGAGS
                     SSSPCDATYRGPSANSEPETQAVTAYILANFPDQRGTGAAPANTTGIFIDLHSYGGYV
                     LWPWSYTTSSAPNSTQLATLGRKMAFFNSYLPGQTGPDLYTCSGVSTDFSYGELGIAA
                     YTIEMGTAFFQSCSTFENTIYPTNLQALLYAAKVARTPYMTPLGPDSLNLTLSASTVP
                     AGTPVTLTGQANDTRYRQTNGTEPTQNISAAEYTIDTPPWESGASPIAMTATDGSFNA
                     TTENITGAINTSSLSTGRHTIFVRSKDANANWGAVSAVFLTIEGPVNQAPVVSGIPDQ
                     TISEGESFASILLDDYVTDADNPDAQLTWSFSGNSALGVSITDRVAVITAPSAEWSGT
                     ETISFRATDPGGLYSQDSAVFTVTEVNDAPLVSDIPDQSIEEGSSFATIALDDYVSDV
                     DNPDAQITWTSSGSSQLLVSITNRVASISTPNAEWNGSETITFRASDPGGLFAEDNAS
                     FTVTAINDPPVVADIPNQTVPEGGSFVSVALDDYVADVDNADAELSWTFSGNSSLQVS
                     ITDRVATIALPAPDWTGSETITFTAADPAGLSDSDEALFAVTVGNKAPDVSDIPDQTS
                     AEGGSFASINLDDYVTDQDNTDAELSWSVSGNSALSVTITDRVAVINVPDAEWSGSET
                     LSFRASDPGGLYDDDTAVFTVTAVNDAPVVSDIPDQSVPAGGTFAAIILDDFVSDIDN
                     ADAELVWSVSGAVELTVTITDRVAAINAPEDWAGTETLTFRATDPGGLYAEDAAFFTI
                     RAANQAPIVSDIPDQTIAEGGTFVSINLDEYVADPDNEDAQLTWTSSGNSALLVSIIN
                     RVATISTPDSNWNGSETITFRAADPEGLFSENPAVFTVTAVNYLPVVLAQEVATQAGK
                     ALPIILSGTDADNDLLIFSITTQPIHGTLAAGDTSAEWTYTPADGFTGVDSFAFTASD
                     GYGNSEPAVITITVTPAGPIEVFRDDFETDLGWVRNASGTDTATLGYFERANPETNVY
                     NGDKQLGTTVSGSYDLVTGPLAGSSAGDYDLDGGKTSMRSPAITLPTGRDLSLSFSYY
                     LAHYTNSSTADYLRVYVVGSSSVKVFEELGAANDDDAVWASFEADISSFAGQTVRILI
                     EAADASTASFVEAAVDDVLILATNPNNAPTANPQSLTTAEDTALPITLTGTDPDGNSL
                     SFAVTVTPAHGTLSGTAPSLTYTPAANYAGADSFSFTVNDGKLTSAPAVIDISVTPVN
                     DAPLAAAQSVSTTVDTALTIKLTGTDVDGDALTYILLTNPAHGTLSGTAPTLTYTPAA
                     GYTGSDSFSFKVNDGTADSASAAVSITVNPAGPTTIFSDDFENNLGWVFNPNGTDTAT
                     RGAFVRANPESTTYSGNKQLGTTTSGSYDLVTGPLAGSSANAYDLDGGTTSVRSPLIN
                     LPAGRTITLTLRYYLAHYTNSSSSDYLRVQIVGATTTTVLSEVGAKNDDDAVWATLTY
                     NLSSYAGQSVYIVITAADASSDSLVEAAVDDVRIVAQ"
     gene            complement(16966..>18140)
                     /locus_tag="PKL60_04220"
     CDS             complement(16966..>18140)
                     /locus_tag="PKL60_04220"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNW95374.1"
                     /translation="DGIGESNVARVTINIRSNTDPVAYPQTVEAFSAIPLPITLTGSD
                     ADGDPLTFLVVTDPVHGELMGTGANVIYTSEPGYVGPDYFEFVVQDGIGESNVARVDI
                     TVIQANHAPVAEPQSLNATSSVPLPLVLTGTDPDGDALTFQVTDQPDHGTLTGTAPNL
                     TYTSTLGYIGADSFQFVVSDGTLISDPATVNISVSSSGPVTVFFDNFETNLGWVRNPN
                     GTDTAHTGMWERANPEYTWWMGPKQLGSTVSGSYDLVTGARAGWEPGSNDIDYGVTSI
                     KSPSIVLPANSNLTLTFSYYLAHANNSSSADFLRVKVVGSSTTTVLQVLGTNRDKDAF
                     WCTATININTFAGQTVYILIEAADNSSASLVEAAIDNVKVVANPLSILGGTTTDSK"
BASE COUNT         3912 a         4556 c         5430 g         4242 t
ORIGIN      
        1 aagagctgga aaaagtgcgc ggacaccgtc tgcgccaccg cgaacaccgg cgcaaaacca
       61 acgtgcccgt ggtggcttta gtgggttata ccaatgccgg caaatcaaca ctgctgaaca
      121 cgctcaccaa cgcagatatc tatgtcgcag atcaactctt tgccacactg gacccgaccg
      181 cccgccaatt ggagctgccc tccgggcaga ccgtcgtcct gaccgatact gtcggtttca
      241 tccagaagct gccggctcaa ctggtggcgg ctttccgggc gaccctggag gaaattgccg
      301 aagccgacct gctggtccac atcgtggata tctcccatcc aaacgcagcc ctgcaaaaga
      361 agactgtgct ggaaacgctg ctcgagattg gcgcagggga tattccaata attactgcct
      421 acaacaaagt ggacttgctg actgaagaag aactggaaga tgcgcgggta aacctggaaa
      481 accaggccct gctaatcagc gcgaaaaacg gcagcggttt ggacgcgctg ctggatacga
      541 tcgggcggga gctgcttgcc gcgtacgcag aactggatgt cctgctgcct tatgatcagg
      601 ggcagctcgt gtcggtattc catgaaaaag gccacgtgca gaaattggaa cataccgaaa
      661 aagggatctg catccacggc tacattccca gggaacttca ggcacagttt ctgccttacc
      721 tgcgcaaggc gcataaagtt aaataaaaaa actccacgcg ccgcgtggag ttggatgaat
      781 acagcggtaa ctaatctttt tcgatggtga gaatttctct accctgatag tagccgcatt
      841 ttgggcagac acgatgcggc aaacgaggct ctccgcagtt ggggcaggca acgctgttgc
      901 gcgccttcag agcatcgtgt gagcgtcggc gatcgcggcg tccggagctg gtttttctct
      961 taggtaatgg ggtcatcttg aactcctgct tatttcgtta acttttctac tatacccgat
     1021 atgcctgatt aatgcaagga aaaatgccgg tttttttgaa tttgtgccgt gttgggtaag
     1081 ctgaataaca aaggctccta tttttgcaga aaggaagcag accgttgaaa atccaggata
     1141 tggatttccg cggctggtaa agtgtctggg tatattgagg ctttttcgat gtttcgcgag
     1201 aaatgcaggg tcaggcttct tttgcgactg tattcgccag ggaagtgcct gccagggcgc
     1261 ccgcggacag ccgcacgccc ccagggcagg cgcagctctc ccatatccgc gcagctgatg
     1321 ttggggattt gggagttacg caccgtgggt aacggacaga taaggtaaca taaatttcgc
     1381 acattaaaaa aaggatatgg tcctcaatca cttggtaaga ttttgttatc accaaaacac
     1441 caacggagaa ggaaccatgt cagaacagat tgtacagcta aacgaacagg taataaggac
     1501 tgaatgaaag gaactggtcg cgcgcttgca tgccgcaaac ccccgatttt tccggtccgt
     1561 ttgtgctggc gcggaataaa accaggccag cgccgcgaat ctcctcctgc ccttcaggat
     1621 ttggagcaat agcaatgctg cggatttcca tgatgatttt atgccgtgca gcgggcgaga
     1681 aacgcaaaaa atggtcaaca ctctggcagg caaacgtaac ctgacagtat gaatacccgg
     1741 gccgcagaat cattgtttat ggattggggg ttggggtcag cgtatcgcgc ggcaaagtct
     1801 cggaaggacg cggtgtgcgc gtgggggttg gcggaatcgg cgtagcggtc gggggttctt
     1861 ctgtggtggg ttctaaggtg ggcgggagtg tgggcagctc cacggttggg gagggaatga
     1921 actgcgtggg cagcggcaag cggctggtgg gggtcttgaa ggcattctcg tcgggaataa
     1981 tgatggtttt ggagcagtgc gttccgtttg cgtcggtaaa ggcaatcctg accctgtctt
     2041 tgttttctgc gagcatttct acatataagc gggtcagcac cgccgcgttg cgcatgaagg
     2101 taccgtagga atatacgcgc gtgtcgtttt tccagagctt aaagtcgggc aaattgagcc
     2161 gcatggtcgg tttgccctgc gcatccaaca cctgataatt gctgaaatct ttatcagtat
     2221 cgaaaacaat ttggacgaca tcgttgatga agaacgagat gctgaagtac ttaatatcct
     2281 ccgcgctgca aagtgggtta gcggtcggcg tttgggtgag cgtagcggaa gaagggggag
     2341 caggcgtgtc ggtgaaggta aaagcctcgt ctgtgctcgg tgtaaagctt ttgattatgg
     2401 agaacaggct gtaaggtgaa ggcgtgataa atcccaggcg ggtgagcgcg ggcgtctggc
     2461 tgtaaacgcg ggatggggac gggttagcgc ctaaaaagtt ggcgaacgcc gagctgagct
     2521 caggaatgac cagcgttgcc accgaaccaa gcaggaagat tgccagaatg aaaacgggca
     2581 gaatccaatg cggcaggcgg ggtttgaaag tcgaggcgct ggtcaggcgg atttccgggg
     2641 gcagtctctt atacctggtg gaagacagca tcggacattc tgaatagcgg gagctcaggc
     2701 aaaaatcccg ttgatggatg aaaaccggca gcgccagcgg ttggctgtga tggcagtaat
     2761 taaggtccga agggaattct gaataggaat ttacatcatt gatgagccca atataggggc
     2821 agcgcaaata ttcccgcgga tagccactgc ttggattgtc ttcggtcgtt gtcattacag
     2881 gtctgctcga tgatggatta aagataacta attttatatc aatttttctg aaaacgaaca
     2941 aaagccgtca aaacaagcag attatgtcag tatttttccg gattcgagat aagccattga
     3001 aacaagagtg gaaacttttt ctcgatctgg tcctcaatct catgggcgac ctcggtgggt
     3061 gagtccccgg agaggtaagg gtcaagaatg ttgaaagggg tgccgccgca cacctctgag
     3121 agcagataaa ttctgcttcg cacagccgga aattccatct gaagcgcttc cttgtggccg
     3181 gcttccatta ccaggatgag gtcgaattgt tttaccagct gggctgagat gagcttggag
     3241 cggtgattct ccagattgag ctggcgtttg ttggcttcaa ggacggcttc gggcatggca
     3301 ggcaggtttg ggtctcccca ggtggcagcg ctttccacgc gccagttcgc cgcatctgcg
     3361 gttcggcgca gtttctcctg aaacatcacc tgcgccagcg gcgaacgaaa gcggttggct
     3421 gtgcaaacaa aaagcagggc gggcatactt agtcttcctc aactaatagc gataccaata
     3481 ggttctctcc ctcgggatga ggatccactc gcccggttcc agcgcatctt caggaagtaa
     3541 atcattgtac cgggcaagaa tgtctaaatc tacattgaat tgtccggcaa tatcccgaag
     3601 agtcaagccc ttgtccgcaa tttcaaaagg ctcaaaagca ggtaaatcgc ccggatcctt
     3661 tctatccagg ggaattacga taatcgaatc tacccagata gggacaacca gattgtaatt
     3721 taccgattta atgacgtctg gcgttgtttg gttctgggtg gcaaagagtt ggatgctttc
     3781 accggaagat acgcggtgaa taatgaatcg gtcctgcctg ccgatgacca tatccagctt
     3841 gtgcggcgca cgggttgttt gcgctgtggg ggtaagcgtg ggggggacag gggtgctcgt
     3901 cggcgtcagt gcaagggcgc gggtgggggt ggcggtggcg tcccggatga gcaggaatgg
     3961 aactgctgtg tgcgtaggtg tggcggtggg gctgggggct gcggtctgtc cggaattgcg
     4021 cccattggtc agcaggaaca ggcaaacaaa aagcactgtc gcggctgcca ggcttaagaa
     4081 aagccagcga aatggtttcc gggtgggacg ggtttctttg atcgttaact caaccggcat
     4141 tttgctgttg tgttcggcgg tgtatacctg gcagcgcgcg tattctgcct tcaggcagca
     4201 gtttgcctgg tgggaaaggg tcggcgttgt tggcggggag acgtgatagc acgcattgtt
     4261 taccgaagga taatcctgga cgctgtccga gtcttcagcc agcccaagat acgggcatat
     4321 gccagattgc ccggcgttca agcctgtaga atcccactcc ttagagttca tcggtgcctt
     4381 caaactaaac tgatagctcc cagccttgat tccccgccag gaagaaaagc actacttgcg
     4441 tttcaacggg gcggccgaag cttttccatc cgtcagacct aaatcaaaac cttcttcgat
     4501 ggacgtgctc tggtggtagc cctggtgact gctgtaaggc gaataataat tgtatccgcc
     4561 gtagtagtaa tccctgttgc gcggaattct gttcatcaca acgcccagca agttcgcgtt
     4621 gatgcgctcc agctcttcca gcggcctttt tgcggctacg ctgtgtgtga gccccggcac
     4681 tatcacgtaa atcacgccgt ctgtccgcgt ggaaagaatt tgcgcatcag agacgagcat
     4741 gggcgggctg tccaaaacca cgatgtcgta caatgttttc aactcttcaa gaattgtgcg
     4801 catgcgttca ctgccaagca tctccgccgg gttcggcggc aaaccgccgc tggtgatgat
     4861 atcgagatta ttaacctcgg gcagcgtccg gacgacattc tcgagcgtga gctttccgcg
     4921 cacaaggtcg ctcaacccga cgcgattatg aatgttgaac tgggcatgca cgtttggtcg
     4981 gcgcatgtcg gaatccagaa gcagcacctt cttgccgctc tgcgccagga cgatggccag
     5041 gttggacgtg acggtggttt tcccttcaga ttccccggcg ctggtaacca aaatgcagcg
     5101 cagaggtttg tcaatgctgg agtattcaag gttggtgcgc agcgagcgaa acgcttcgga
     5161 taccggtgag cgcggatgat aggcaacata attgccaagc ttttcgttct tggatttttt
     5221 gtttagttcg ccaatcagac cgataaccgg cagatgcagg atttccttga cttcgtccgg
     5281 ggtcttgatg gtgtcatcca aaaattcgac caggaaagca atcccggcag tgaccaaaaa
     5341 cccgatgccg ccggcaagca gcgcgctttg cattggtttg ggggagattg gactggatgg
     5401 aacgacggcg gattcaacct ggacaacgtt gggcgtattc tgcgcgcggg tgaggcgcaa
     5461 agtttcaagg ctgctgatgc tgctgatgta gatttgctgg taaagctcca gcgtggtccg
     5521 cagttggtcc agttgggtgg tggtgttggc gtcactctgg gcaggggtct tcaggacgac
     5581 caggttggtg tatatctgct ggtaaagtga aaggacagga gtgagctggt ctaactgggc
     5641 ctgataatct gccaggagag taatttctgc gtccgtggct tgctccgggt caattttcgc
     5701 gatttccgtg ttcaacccgg taatctggtt ttgcaggtcg tcaatctgag cctgcacgct
     5761 ttgcagttgg gtctcaacgg tagcagagga aatttcatta atctgagcct ggagaatgct
     5821 gatttgttcc tgtgcttcat cgatgcgggt ctgcaggttt tgttccgcgc tgatatagcg
     5881 taccgactgc agctgctcgt tctggtcaat cagcaccttg accaagccgt tagcaatcag
     5941 ggcagccctt tccggatcgg tgtcccttac agtaagctgg acgaactggg tgtcgtcaat
     6001 ttgggcggct gtagcctgac ctttttgcac agggaagcca agctcctggg aagcttgcgc
     6061 gagcaggctc tcggtagaaa gcagctgcgc gtaggtggaa atgagctgct ggctgtccag
     6121 gtatgaaaag taatcgtaag tggtttgcgc cgcacgaagc acgacaaagc gggtggaggc
     6181 ctggtagatt ggtgtctggc ggttgctaaa gacaaatccc cctgcagcgc cgaggattgt
     6241 gcctaacacc aggaaccaca accagcgctt tgccgcgaca aatatctgtt tgatatccat
     6301 aaactatctc catttacccg ctggtctgaa gcggcccttc gatgataacc gtcccaccct
     6361 ggatcgggcg gatggtgttt cttttcagtc gcccatgtca ttgcacccga atgccggact
     6421 aaacattatc ctgcaaagct ttgcgaaaaa tcatccgcaa gcaggctatt aaacttgcgc
     6481 agcttgaatt atattcagtt tcaccagccc tgtaaaccaa gcaaaaacct gcccggaacg
     6541 acccgggcag gtttcagaat cacaaaacct tacttggcaa gcatcggtaa aaacaatggc
     6601 atttccacct ggatgctgat aacggccggt tctgagtagg ccaggccgtc ccagacctta
     6661 aagctgaagc tgtcggcgcc gatgtaacct gggtccgggg tgtaaaccaa atccggaagg
     6721 gcgccgctga gctggccgcg gctggggttg gtcacaacct cgaaactgag cgggtcgccg
     6781 tccgcgtccg agccggtaag cgtgatgttc agcggcaggt cccgccgggt cttgagggat
     6841 tggccgtctg cgaccggtgg ggtgtttacc ggcccttcga tcgtcaggaa aatcgcgctg
     6901 acagcgcccc agttgttcaa gttatccttg ccgcgcacat aaaggatgtg ccggccgggt
     6961 tgccaaccgg tggtattgat gctggccgtc acgttttcgg agctggagtt gaagctgccg
     7021 tctgccgggg agagcgcgac tggcgcgtag cccgcttccc agtagggcag gtccgcggaa
     7081 tactgcgctt cagcgatggg ctgggtgggt tcgccgctgc cgctcttgta gcgggtatcg
     7141 ttaacctgac ctgtcagcgt gacgatagta ccggccggaa ccgagctttg gctgagcgcc
     7201 agattgatgg aatccggccc ggcgggggtc atgtacggcg tgcgggcggt tttggcggcg
     7261 tagatgaggg caggcaggtt gccgggtaaa atatcaaact cgaatgtgtt gcagctttcg
     7321 aaaaaagcat cacccacctc aaagcagtag ccagccacgc ccaggcgccc atagatattg
     7381 tcttcggtgc ttccgtccgt aatgtaaagc tgccaggacg gactaggggt gtaattgttg
     7441 aagaaggcga acttgcgccc gagtgtctgc aaatcggtcg cgtttggggc gggattttcg
     7501 gtgaagcccc aggaccagag cacccttctt tcataactgt gcaggtcgat gaacacgccg
     7561 gtggcatcct cggcggcggg catgttcagc gggtcgccgc gttggtcggg gaaaatcgcg
     7621 gtcatataat tctgaacggc ctgggtttca ggttcggagc cggcgctggg gccgcggtag
     7681 gtttccgcgc attggctgcc gcttgagccg ccgcagcagc cccagtagaa gctaaagttg
     7741 cggttcaggt cagccccgcg gttggaactg gttggaccgc agtagttttc gttggtgttc
     7801 ttgcgccacg acatgccgct ttccgcttcc ttacgtccgt ccggattggc ggtgatcatg
     7861 atgtgaatct catggtaatt caagagccag tggacgtcgg cgttggtctg gtaattttcg
     7921 atcaggtatc cggcaaagcg cgcagccagt tccgccggcg cgtattcccg ggcatggatg
     7981 gaagcggtga tgaacagctt gggcttgtcc ccggtgatgc tttcgttcgt cagacgcagg
     8041 acgtacatgt catagccgtc cggctgccca acgctcttct cccacgagtc gccgacgtcg
     8101 gtccattccg ccaggtttgg ataggtggac gccagcgcgg cgccgtaggc ataaagctct
     8161 tccactgtgc ggtagcagtt atatggcggc ggcagagttt cgagaccctg acttgcgagc
     8221 atatccagct tgcggtagtt ggcggtctgc tcctcatcca gaacgacggt gaagcccatg
     8281 ctttggatgg tggtaatttc tgaagcgctt gccgccacca gcacgtactt ttccgcattg
     8341 ttattaaact cgaacagatc gtaggatttg agttttgaaa tatcctccaa ggactggtag
     8401 tacacgcggt aaacctgttc cagttctgct tccgcgttgg tacaggctgg tctgtccgcc
     8461 gtcaacgctt ccgccgcatt tgcgcttcgt tcgccggtga ttgacaccag caaaaccagc
     8521 gcgaacaaac ccgaaaacca cagctgtctt ttcataacgc ctctctattc tggttttagc
     8581 cattcatgaa tacgaaaccg gtctttctcc tgctcgatat cctgataaac tgcctggtcc
     8641 attgtcgata ttttcctgac tgtgcggccg tattggcgga ataaatcgaa tgccctgcgg
     8701 atataacccg gcacgatgcc cgccgagtcg gggttgagtc tatacagccg cgccagagcc
     8761 ggtctgggta tgaatattct gttaagaatc agtctcgctt tttggacgag gtttttcgtg
     8821 gaggttaatg cggcgacgtc aggtgtaatt gcattgcgtg cgcgcttatc ctggagggtt
     8881 aatgcttttg ccttggttag tatatcagta tccagtgagc gtggaagcaa ttctgacaaa
     8941 aatgcttctg gaatctgtgc ccagcccatt tcctgcagca aagcaaagct taaggcagca
     9001 gttttttcgg cgccccattc ccgcgcgatt tcaagcagtt gtcgccaatc tatgctgtca
     9061 gcctcgctct ccagaaccac agcgatatcc aggagcccgc gcaaaccgag tttgagatag
     9121 tgctggtaac tggtatggat acaaagatgc aagagcaaat cttcagtgga aagcgctaat
     9181 gcatccgcgt ccgcaaagcg ggttgggcga gcccgctgcc atactccctg ggggtttatg
     9241 gagaaaggtt catcctcctc cagaagcgtc cagtggactt cgagcgtggt tccatctgcg
     9301 aggctcatcg gcggcacgtg tttcacgtcg cgattatccg cgctgctgtc gaaatatgaa
     9361 ggcgcttgat aggaaagcct tcggagcgtt tcgattgtac gggggacgtc ttcccggtgg
     9421 acgagaatat ccaggtcatt catggctctt gcaccgatat ccctgtaaac gttttccagc
     9481 agatacaagc ctttcaatcc gatgaccgct gtgccttgtg cttgcagcgc tgccaggact
     9541 tcggccgcgc gcgtcagaag caggaggttc cgggcagcgt ttgccagata attctcgcgc
     9601 agcgcgcggc gctcgtctgc tggaaccagg tcgagcagct cgttctgctg caagcgggta
     9661 tagagcaggt tggccagtga gtgtgtttcg agatattggc tgaagttttg ccagtctccg
     9721 ctgggaaatc cgcccaaagc ggcagccagg gtctcgctcg tgctcagggg gtttaataac
     9781 tccagcagga gtgcgggaat gtcgcttttg ggatttccat cgggtgtcgg agccatggct
     9841 ggatacaatc ttgttctctt ttttcctttt ttcgtcagca gtccagtcgt aaagtcagcc
     9901 aaactgctta gttttctgcg attttttgca ggattggcat gtaaaccatc actccggtct
     9961 taatcatctg cggaccgtaa aacacgccgg tgggtccata tgcgtcctgg tcctccaacc
    10021 aataccagta cacttgcccg agcactgcgc tcgcgtccgt gaaggcatac tgcgcgtcgt
    10081 tgacagagcc gcgcgggatc aggctggcgt ttaacttggt ttttggcccg tccagcgctt
    10141 ccgcgcgata caggttgaag ccgagcgcgt cgcccagtgt ggaggcgctc caatggatta
    10201 gattgccgga gggcgtgtat tccgcgctta acccggtgta gaccaccgaa gatgggtggg
    10261 tgaccgtcca ggtgtaggta tccagcgcgc tgttctggtt atagtcccag gcctgcacct
    10321 ccaccgtgtg cgggccgttc tggctgggga agcgccgctc gccgagcatg gtgaagccga
    10381 ggtcgatgta gaactcgcgg catccgtaat cgccgcaggt ggggggagaa atgtaaaaat
    10441 catcgaccag ctgggtatcg ttgctgccgc cggggaatgt gtgaaactcc cacaaggtcg
    10501 tccacggccc gccatccagc gagtaggcga ttttgtaggg aggcagagta taaaccgggc
    10561 tgaggaagag gtcgccggca tgcgcgtaaa ggccgtaagt taggatttct ggttcagcga
    10621 tggtatccct ggtaaggatg gtattgttca aggtcagacc caggtcttcg attacaggcg
    10681 tttgggtatc cggcaggagc gtatggaact ccagcgggtt caggtacacg tcgccgtcag
    10741 ccagaatatt gagatgaata tgatgaaaat agtacccaaa ggatacaacc ggccagaaaa
    10801 caatttcgcc cagttttgtg tttgggggga tgaaaccccc gctttcatga tcggcctgcc
    10861 aggcctggta agcctgataa atcgctgccg ggatgctggg ctgagcgatg tggtggtact
    10921 gccagacata accttccgcg tccagaatgg ccacttccca gtagagcccg ccgtaatcgt
    10981 agttctcgat gttgacgacc tgtccgcccg agcgcgtgaa gacttcggtc tcctgcgggg
    11041 caagcatatc gatgccgtga tggaaatagg gcgcgccgcc gtaatactgg taggactgga
    11101 tcacatgccc catctggacg acggtaaaag gccaggcgta aaccgaatgc ggtccgtccg
    11161 gattgcccca ggccagcccg gcggagccgg tctggatgcc ggcgcgctct tgcgcgggct
    11221 cctcaggcgg ctccgtgggt tgtgctgacg ccaatcgggt ctggaagcca gccaaaacga
    11281 tgagcagcag gatcagcata aactggataa gccgtttgag ttcctgaatt ggtttgggca
    11341 taaaaacctc tggggagttt tgcacgggat tattttgatt ctatcaccgt atacctacac
    11401 gggaagaaat ctgggaaaag catgcttttt ttcctgaaaa gcagaggtca gaaccaggaa
    11461 atccggttct gacctcattg cttatttcag tatagtttga gtgggcttgc ttcgtcctca
    11521 agcctttcag agcttactgc gcgacaatcc gcacatcatc aacggccgct tctaccaagc
    11581 tatcgcttga ggcgtctgca gctgttatca caatgtaaac ggactgcccg gcatagctgc
    11641 tcaggttgta agtaagcgtt gcccagacgg catcgtcgtc attctttgcg ccaacttccg
    11701 aaaggacggt ggttgtcgtc gcgccaacaa tctgcactcg caggtaatct gagctggaag
    11761 agttggtata gtgcgccagg tagtacctga gtgtcagggt aatcgttctg ccggcaggca
    11821 gattgattaa cggtgagcgg acgctggttg taccgccgtc caggtcatac gcgttggctg
    11881 aactgcccgc gagcggcccg gtcaccaggt cgtatgaacc gctggtggtt gtgccaagct
    11941 gcttattgcc ggagtaggtg gtagattcgg ggttagcccg gacaaaagcg ccgcgtgtgg
    12001 cggtatccgt gccgttgggg ttgaataccc aacccaggtt attttcgaag tcgtccgaga
    12061 agatcgttgt aggacctgcg gggttgaccg tgatgctgac tgccgcggaa gccgaatctg
    12121 ccgtaccgtc attcaccttg aagctaaaag aatcggagcc ggtatagcca gcagcaggcg
    12181 tgtaggtgag agttggggct gtgccgctca gggtgccgtg cgcgggattg gttaggagga
    12241 tatacgtgag tgcgtcgcca tcaacatccg tgccggtgag tttgattgtg agagctgtgt
    12301 cgaccgtggt gctgacggat tgtgcggcag caagtggggc gtcattgaca ggcgtgaccg
    12361 aaatatcgat caccgcgggc gctgaagtca gtttgccgtc gtttacggta aagctgaagc
    12421 tgtccgcgcc tgcgtagttg gctgctggtg tgtaggtcag gctgggtgcg gtaccgctga
    12481 gggtgccgtg cgcgggagta accgtcacag caaagctcag gctgttccca tcagggtctg
    12541 tgccagtcaa cgtgatgggc agggccgtgt cttcggcagt tgtcaggctt tgcggattgg
    12601 ctgtcggcgc gttgttcgga ttggttgcca ggataagcac atcatccacc gcggcttcca
    12661 cgaagctagc tgtgctggcg tctgctgctt caatcaggat tctgactgtc tgaccggcaa
    12721 agctgctgat gtccgcttca aagcttgccc aaacggcatc gtcgtcattg gctgcgccaa
    12781 gttcctcaaa gaccttaacg ctgctgctgc caactacata aacccgcagg tagtccgccg
    12841 tcgatgagtt ggtatagtgg gcaaggtaat aactgaagga aaggctgagg tcgcggccgg
    12901 taggcagcgt aatcgccggc gagcgcatgc tggtcttccc gccgtccagg tcgtaatcgc
    12961 ctgcggaact gcctgccaag gggccggtaa ccaggtcgta ggagccgctg acggttgttc
    13021 ccagctgctt gtctccatta tagacatttg tttcagggtt agcgcgctca aaataaccaa
    13081 gtgtggcagt atcggtgccg ctggcgttgc gcacccaacc taaatccgtc tcaaagtcat
    13141 cgcggaagac ctcaatcgga ccggcaggag tgacggtgat ggtgatgacc gcgggttcgg
    13201 aattgccgta gccgtcgctg gctgtgaagg caaaactgtc cacgccggta aaaccgtccg
    13261 cgggagtgta ggtccattca gcggacgtat cgccggctgc aagcgtccca tgtataggtt
    13321 gggtcgtgat ggagaagatg agaaggtcgt tatccgcgtc agtaccgctt aggattatgg
    13381 gaagcgcttt accagcctgc gtggcgactt cttgcgctaa cacaaccggc agatagttga
    13441 ccgctgtgac ggtgaagacg gctggattct cactgaaaag accttcgggg tccgcggcgc
    13501 ggaaagtaat cgtctcgctg ccgttccagt tcgaatcggg cgtgctgatg gtggcgacgc
    13561 ggttgataat gctgaccaga agggcgctat tgccgctgga tgtccaggtc agttgggcgt
    13621 cttcgttatc cgggtcagcg acgtactcat cgaggttaat gctcacgaac gtcccgcctt
    13681 cggcaatcgt ttggtcaggg atatcggaga caatcggcgc ctgatttgcc gcgcggatag
    13741 tgaagaaagc tgcgtcctct gcatacagcc cgcctgggtc ggtggcacgg aaagtcaggg
    13801 tctcagtgcc tgcccaatcc tcgggagcat tgatggctgc gacgcggtcc gtgatggtga
    13861 cagtcagctc cacggctccg cttaccgacc atacaagttc agcatcggcg ttgtcaatgt
    13921 cgctgacaaa gtcatcaaga atgatggcag caaaggttcc accggcgggg acgctctgat
    13981 ccgggatgtc acttacgacc ggggcatcat tgacagcggt gacggtgaag acggctgtgt
    14041 cgtcgtcata cagaccgccc gggtcgctgg cacggaagct tagcgtttct gagccgctcc
    14101 actccgcgtc cggaacattg attaccgcta cgcggtcagt gatggtcacg gagagagcgc
    14161 tgtttccgct gactgaccag gacaattctg cgtcggtatt atcctgatcc gtaacgtagt
    14221 cgtcgaggtt gatgcttgcg aagctgccgc cttcggcgct tgtctggtcg ggaatatcgc
    14281 tcacatcggg agctttattc ccgacggtca cagcgaagag cgcttcgtcc gaatcgctca
    14341 gcccggctgg atctgctgcg gtgaaagtaa tggtttcgga accagtccag tctggagcag
    14401 gcagcgcgat cgtcgcgacg cggtcagtga tgctgacctg caggctgctg tttccgctga
    14461 atgtccagga gagttcggcg tcggcattat cgacgtccgc gacatagtcg tccagggcaa
    14521 cgcttacaaa actgccgcct tccggaaccg tttggttggg aatgtccgcg acaaccggcg
    14581 ggtcattgat tgctgttacc gtgaagcttg cgttgtcttc ggcgaacaag ccgcctgggt
    14641 cgctggcgcg gaaagtaatc gtttcagaac cattccactc ggcattcgga gtggagatgg
    14701 acgcaacgcg attggtgatg ctgacgagga gctgagagct gccagaactc gtccaagtga
    14761 tttgcgcatc gggattgtcc acatcgctga cgtagtcatc cagggcaatg gtcgcgaaag
    14821 agctgccttc ttcaatgctt tgatcgggga tatccgagac cagcggagca tcgtttactt
    14881 ctgtgacggt aaagacagcg gaatcctgag aatacagacc gccagggtcg gtggcgcgga
    14941 agctaatggt ttctgttccg ctccattcag cacttggcgc agtaatcacg gcgacgcggt
    15001 cggtaatcga gacgcccaaa gcggaatttc cgctgaaaga ccaggtcaac tgcgcatccg
    15061 ggttgtccgc gtcggtcacg taatcgtcca gtagaatgga tgcaaagctt tcgccctcgg
    15121 agatggtttg atccggaatg ccggacacaa ccggggcttg atttacgggg ccttcaatcg
    15181 tgaggaaaac agcgctcacc gcaccccaat tggcattagc atccttggag cgcacaaaaa
    15241 tggtgtggcg gcccgtggag agtgagctgg tattgatcgc gcctgtgatg ttttctgtgg
    15301 tcgcgttaaa ggaaccatcg gttgcagtca tcgcgatggg agaagcgccg ctttcccacg
    15361 gtggtgtatc gatagtgtat tccgctgccg aaatattctg ggttggctcc gtaccattgg
    15421 tctggcggta gcgggtgtca ttcgcctgcc ctgtcagcgt tactggcgtg ccggcaggca
    15481 ccgtgctcgc gctaagcgtg aggttcagcg agtccggacc cagcggggtc atgtaaggtg
    15541 ttcttgctac cttagccgcg taaagcagcg cctgcaggtt ggtcgggtag attgtgtttt
    15601 cgaaagtcga acaggattgg aagaaagccg tgcccatctc aatcgtatag gcagcgatac
    15661 ccagctcacc atacgaaaag tctgtactca cgccggagca ggtgtagagg tccggtccgg
    15721 tttggccggg cagatagctg ttaaaaaaag ccatcttgcg gcccagagtt gccaattgag
    15781 ttgagttagg cgcagaagaa gttgtgtaac tccagggcca gagtacataa ccgccgtaac
    15841 tatgcaggtc gatgaaaatg ccggttgtat tcgcgggggc cgcgcctgtt ccgcgttgat
    15901 ccggaaagtt cgccaatata tacgcggtta ctgcctgtgt ttcgggctca gaattcgctg
    15961 atggaccgcg gtaggtagca tcacaagggc tggaactgga accagcgccg ccccattggt
    16021 agcttaggtt gcggttcagg tcagcgccgc ggtttgaact tgtcacgccg cagtaattct
    16081 cattggtatt cttgcgccag gatactcctg tctccgcttc cttacgtcca tccggattgg
    16141 tctgcagcag gatgtgaatc tcgttgtaat ccaacatcca ggtgatgtct gcattggtgc
    16201 cgtagttggc aattaagtat tcggcaaaac gggtcgcggt ttcggctgtt gtgtattccc
    16261 gggcatgaat ccctgcgatg atgaacaatt taggtttcgt cccgctgatt tgggaattgg
    16321 tcagcttgag caccatcatg tcgaagccgt caggctgtcc gacgctcttt tcccaggaat
    16381 ccccaatatc aatccattgt gcaagcgtag gattgttggc ggccagagct gccgccgccg
    16441 caaaactttc ctcaacggtg cggtagcagg aataacctgg gatggtctga atcccgtttc
    16501 cgaaacccaa ggccaggtcg gctgtacgtt cttcgtcgac ggtcactttg aatcctaagg
    16561 cctggatgcg ttcgatttca ctctgtgtgg cagacacaag cacatacttt tccacactat
    16621 cgttgaattc gaacaggtca aacgggacca gcttttggat atcttcctgg ttcgtgtagt
    16681 aaacccgcac gacaaaaacc tgggaagtgc tttgctccgg agtttctgcc tgcgcgtctg
    16741 cagagcttac cagtagactg ctgaagctgg aaacgatgag acaaacaatt attaacattg
    16801 tggacaacaa acgatgtttc tgcatgtcta gctccttttt ttatgttgaa aaaggtaaga
    16861 atgaactgaa cattaacgtg catgtcctat gccaaaaaca gagtaacttg tcgaaagaga
    16921 aatccggttg tgatgatacc catcacaacc ggattaactt aaggtctact tggagtcagt
    16981 ggtagtgccg ccaaggatgg acagcgggtt ggctacaacc ttgacattat caatcgctgc
    17041 ttccaccagg ctggcactgc tgttatcagc tgcttcaatc aggatgtata cggtctgccc
    17101 ggcgaaggtg ttaatgttga tggtcgcagt gcaccagaag gcgtctttat ccctgtttgt
    17161 acccaaaacc tgcagcacgg tggttgtgct tgaaccgaca actttcacgc gcaggaaatc
    17221 tgcgctggac gaattgttag cgtgcgcgag gtagtaacta aaggtcaggg tcaggttgct
    17281 attggcgggc agaacgatcg acggggactt tattgaagtc acgccgtaat cgatgtcatt
    17341 gcttccgggt tcccaacctg cgcgggcgcc tgttacaagg tcatatgatc cgctcacggt
    17401 gctgccaagc tgcttcggac ccatccacca ggtgtactca ggattggcgc gttcccacat
    17461 gccagtatgg gcggtgtccg ttccattggg attgcggacc caaccaaggt tggtctcgaa
    17521 gttgtcgaag aagacggtaa ctgggccaga agaagatacg gagatattaa ccgtagctgg
    17581 atctgagata agggtgccat cagagacgac aaattggaag ctgtccgcgc cgatgtaacc
    17641 taaagtagag gtgtacgtca ggttcggagc agtgccggtg agcgtgccgt ggtcaggctg
    17701 gtcggtgacc tggaaggtca gagcatcccc gtcaggatcc gtgccggtaa ggacaagcgg
    17761 aagcgggacg ctggaggtcg cattcagaga ctgcggttcc gcgacaggtg cgtgattcgc
    17821 ttgaatgact gtaatatcca cgcgcgccac attggattcg ccgatgccgt cctgcacaac
    17881 gaactcaaag tagtccggac caacataacc cggttcagat gtgtaaatta cattggcgcc
    17941 tgtacccatc agttcaccat gtaccgggtc cgtaacaacc aggaaagtga gcgggtctcc
    18001 gtcggcatca gaaccggtta aggttatggg taacggaatt gcgctgaaag cttcgacggt
    18061 ctgggggtag gcaaccgggt cggtgttgga gcggatattg atggtcacgc gcgccacatt
    18121 ggattcgccg atgccgtcct
//
LOCUS       DAOQAD010000033        17974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_3285_length_17974_cov_6.241029, whole genome shotgun sequence.
ACCESSION   DAOQAD010000033 DAOQAD010000000
VERSION     DAOQAD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 17974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17974
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3285_length_17974_cov_6.241029"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1483)
                     /gene="der"
                     /locus_tag="PKL60_04225"
     CDS             complement(104..1483)
                     /gene="der"
                     /locus_tag="PKL60_04225"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNW95375.1"
                     /translation="MKKPVIVLVGRPNVGKSTLFNRLIGERLAIIDDVPGTTRDRLVA
                     EGDWSGHDFFVVDTGGIDPTRQRSQEPLSIGSAEFIADIRVQAEIAMEEADVILLLVD
                     AQQGVTEADREIVQMLRRRRPAAAGQSIPPIILVVNKADSELTRANALDFYSLGLGEP
                     FPISAIHGTGTGDLLDEVVKHFPQPAEDEEEDNSIKVAIVGKPNAGKSSLLNKLAGEN
                     RSIVSPIPGTTRDAIDTQISWEGLPITLIDTAGIRKRGAIIPGVEKYSVLRAMQAIER
                     CDVALLVVDAVTGITAQDTHIAGYINQAWKSAILIVNKWDLIEKDTHTSVEFEKTVRQ
                     ALNFLPYVPVMFISAASGQRVNQVLPMVARVQEERLVRLTTSQLNRILQNAQDMHQPS
                     SGSGRMFRIYYGTQVRSEPPTFLLYCNDPTLGHFTYLRYLENQIRKEYPFVGTPIRLV
                     LKARERGED"
     gene            complement(1516..2739)
                     /locus_tag="PKL60_04230"
     CDS             complement(1516..2739)
                     /locus_tag="PKL60_04230"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNW95376.1"
                     /translation="MKTLRWIVKFLPTLFTALILAIFVWVSAVSSTDPNEEREYATPV
                     PVTVLGLDPDLVITGQLEDEVKVRIRAPRSVQERLAANPDAVHAYINLSGLTAGEHTL
                     EPQIAIDLSPTRVTLVTPPEITLTLENILSKEVPVELRLTGSLPIGYEAGEAVLSHDT
                     AVAIGPESQVLKVERVVAGLDLNNVSTSISRQVELKALDARGVIVSGINLNPTQVTVE
                     IPVTQLGGYRNVFVKIVTTGQIAQGFYLTGILADPPTITIYSTDPELAANMPAFIETT
                     PLNLNGASESFQKDVNLNLPEGIVVVGDQQVSIHVSISAIQSSILLTGIPVEISNLGQ
                     GLKAALSPDRVDIYVSGPLYLLDKLNAADIIVMLDLANKTPGTYQLIPTVTLADTAIN
                     LDSILPASIEVVITR"
     gene            complement(2736..3623)
                     /gene="cdaA"
                     /locus_tag="PKL60_04235"
     CDS             complement(2736..3623)
                     /gene="cdaA"
                     /locus_tag="PKL60_04235"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNW95377.1"
                     /translation="MQELIDNFLFILERFDWISVIDIALVTVFFAILLYMLRDTEALT
                     LLRGIFLVVILITLVTSLVNLPAFSWLVNTIAPATIFVIPVIFAPEIRRALERVGRFS
                     NIDFLTRRSNPLARERTEITLQEITKAAVRLSERRHGALIVIRRNDFLTEYYKNGVFL
                     NADLSAELLLQIFYPNTPLHDGAVIVEGNRITAAACVMPLSASGVLNSLPERSVGLRH
                     RAGLGISEVSDAVALIVSEETGSISIAYQGRFIRRLDGERLLNTLRAFLLPDEQPGKK
                     AAKAAEKKTEDEKQAGSPK"
     gene            complement(3698..4762)
                     /gene="coaE"
                     /locus_tag="PKL60_04240"
     CDS             complement(3698..4762)
                     /gene="coaE"
                     /locus_tag="PKL60_04240"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNW95378.1"
                     /translation="MPILLGITGNVASGKSAVRQFLENEGALTIDADLLAQSTYLPGM
                     PAYQPILDAFGSDLCYSDGQINRSRLGKIVFKDPGALRELEAIVHPLVTQGIEAVLAR
                     TEVELVVVEAIKLFEAGLHERCDAVWTVTARDELRQERLMNLRGLTEADAMQRIRSQS
                     PQEEKASRSDYVISTDGSFQETYAQTMAGLNKLQAPPAAEILSRAGELFLPLRPEAFP
                     SARALLNRFFPQTWEDEDIYRSLSHRSLLAGFREGEISALCSWKLEFFLGLVQSVIPY
                     TPGSEELKSALSVLAICAGAHLCKALYIPREMIAPASAKQLGFLPGEAAVADVMPLST
                     ANFLRKNGLVPEEVFVRSTF"
     gene            4845..6272
                     /gene="tilS"
                     /locus_tag="PKL60_04245"
     CDS             4845..6272
                     /gene="tilS"
                     /locus_tag="PKL60_04245"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNW95379.1"
                     /translation="MITERILSSLREDCRWDPARPLVVGVSGGADSLTLAHCLLQAGG
                     KFTAAHLDHGLRPTSAEEAEKTRALLASWGLACRVERQDVRVYAREHRLGIEEAARQC
                     RYRFLFRLAEEIGAQAVAVGHTADDQVETVLMHFIRGSGINGLRGIQARSILADFHAE
                     LPLFRPMLGVSRAETEAYCRDHQLAYLVDESNADPSFFRNRLRHEIIPALEAANPAFR
                     RVILRNARALQSDAELLAEIEEQALSKCVREHSADQLVLDAHAFAAMNEGLQRRVLMQ
                     AVLRLRPGLRDIGLEALDRALVSLQAGKTRFDLVGSLEVWNQQGALTLVPKGFRPALP
                     QYPQLAHNERLALRPGETLALAQGWGLRAEILSAKAYKKLPACDKRSPDHAWLNPADL
                     SLPLEVRPARTGERWSPLGMPGKTQKLSDFFVNQKIPQPARALWPLVFCEGSLLWVAG
                     LRIAQAWRLLGDELEVLHLSLQKPQ"
     gene            complement(6284..7243)
                     /locus_tag="PKL60_04250"
     CDS             complement(6284..7243)
                     /locus_tag="PKL60_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNW95380.1"
                     /translation="MQKPQRKSSLPVILILLILIVALAWAGAVSLNRTRAGEGSFLSN
                     LWSKNAEVVEDPYLNGYSQALQIPPYVKTIMLLGSDYTPQNGYRTDVMLLAAFNLKTN
                     EIHLFSLPRDLWVDIPGYYAQRLNVAHAVGGYELLGNTLAYNFGFRPDHYAIAEFSAF
                     KYVVDLLGGLNVQVSEHMEDQCDFTPERWCVVEPGMYQMDSNFALWYVRARLNSSDFD
                     RNRRAQEVARAIAQKITAPANLVRLPTFIESALDVIETDMSISDMWLYIMPLNKFLQP
                     DLAQGFTLGPNEAVGFVTDEGAMVLQPDYGAIQNILKEVFWIE"
     gene            complement(7286..8215)
                     /locus_tag="PKL60_04255"
     CDS             complement(7286..8215)
                     /locus_tag="PKL60_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNW95381.1"
                     /translation="MNPTSDNLSAHLTAATLMQPAIQAWKIYLHDQGRSPHTLKAFTA
                     DLQLLAGFLPTDAQIGAVSTKDLENFLSWLQTDRKVPCSPKTLSRRITAIKSFFRWLS
                     RGGIIPMDPAEKLEQKTVISPLPVVLTQSEQERALDAADALRTSASRDLRPYLLLKLV
                     LETAMKKGECLNLKTNHVELDGPDGPFIFIRYTSPRYRYKERKVSVSPEWVQAYQEYA
                     AVYNIQDAVFPWSQRRLEYLLEDIGNAAGLDKHLSFDMCRWTSALNDLTAGVETDKIR
                     QKLGVSKIQWREVAQKLRSLAKEYGYTLSSPED"
     gene            complement(8632..9666)
                     /locus_tag="PKL60_04260"
     CDS             complement(8632..9666)
                     /locus_tag="PKL60_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNW95382.1"
                     /translation="MRLKRHQMITLIVFGAIALAFVLVLVSGALVSAAANSSAFPYRI
                     SFKDFSATPTPFQPNAFANILEDGTIELLPQPIYTPTPVPVISQDRLPENQLNILLLG
                     CDDFSANGFRTDVIMLLSLNPRHHSISLISFPRDLYVTIPGYWSNRINTAWGLGGFEL
                     LADTFQANFGIHPDFYMMVNYNGFKDVVNSLDGIDVEVTQELQDSCKLDPSGWCIMEP
                     GMTHMDGGTALWYSRSRLTTSDFDRNRRAQDVVKAIFRKAMNLNVVFKLPELYGAYRE
                     YVDTNLQLADILPYMTLASEVMQTMNINQYAIGPETVTNWITAEGAMVLIPNYYAINP
                     IIAEALYLND"
     gene            complement(9698..11827)
                     /locus_tag="PKL60_04265"
     CDS             complement(9698..11827)
                     /locus_tag="PKL60_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95383.1"
                     /translation="MKALLDNLPLLTVFPYALLYFLILLCASLLAVLALRKASASGSQ
                     PAPALRSALFMIFIAQVLLLTLSLLIFQGFDGLVRYFPLVFHALTLLTLIWLSAGLLS
                     DRLNARQTRLPLILSGLTVLAGALSVLAWSTWPADKAFSGSVFSLAWSAAALLVLFVA
                     LVLALRSQRPHKLERVLILLTAALGYLVNILFPLESALPSGVMLSQLLYYPLLISAAW
                     QFRRGEQARAAAVSSPRMASALLDVGLLSRAEEIRASFTHSLSLYLIGDVVGILEKDP
                     QSPALSVGSAYDLISEATLPAFSLPFSQAPKLRRHFEDLVPLIANADEQLAGEKQALM
                     RASGFNTCGPLLLFPFGSRALLCFNPYTQRAWTSADLDALSPLSDKLSQVLERTAAVE
                     AFSALKDESRYLANQMEREKRDLADSLSTREAELAALISQRANDQAAYTAELRVREQR
                     QKYLEAQLEALHEKLQAQQSALSELDALREQKSALERSLQENEQRALSLRRELENARA
                     ALGDLLGKAGSAPAGTETSAPARSDQSAAGAEEHAPENSPTRAALQNKLDELAASFYS
                     RRVAHQLTFGALPELGPAALACVAQVFENLVINAFAASPPESEVAFSLAPARLEDGRP
                     AVEILAAHAGQSLSPQEQQHLPEALLGEHAEASAGIGDFNALGAAARQLSACGGHMWL
                     KSETDQQTLWRALIPLDGALQGLPQTE"
     gene            complement(11905..13335)
                     /gene="gltX"
                     /locus_tag="PKL60_04270"
     CDS             complement(11905..13335)
                     /gene="gltX"
                     /locus_tag="PKL60_04270"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNW95384.1"
                     /translation="MFRTRFAPSPTGYMHIGNLRTALYAYLLAKKHQGVFILRIEDTD
                     QSRNVPAALAAIYNGLALAGITYDEGPDKDGGKGPYVQSQRLPIYRKYAEELITRGAA
                     YYCFCQKDDKPEPEDQPAHPDITRDPCRLLTQAEVQARFARGLTPVIRQRIPEGGSTS
                     FDDHVYGRISIENAQLDDQVLLKSDGFPTYNFANVVDDHLMEISHVIRGQEYLSSTPK
                     YNLLYQAFGWQIPEYIHLPLILREDGAKLSKRAGDPSFEDLVNMGYLPEAIVNYVALL
                     GWSPGNDREFFTMQDLIEAFDMDRIGKSSAAFSMEKLTWLNGEHIRAMDPAAFHQAAS
                     RYYAAEIQKLDTEKISRLIQIRVEKLTDIPAMTSFLVELPEYSVELFENQKSKATLAS
                     SAQVLSAVIPLLGTLESWDNGSLFAALKEFASAQGYKIGTVMWPIRTALSGLPASPGG
                     ATELADLLGRQETLRRLTIGLEKLES"
     gene            complement(13468..14136)
                     /locus_tag="PKL60_04275"
     CDS             complement(13468..14136)
                     /locus_tag="PKL60_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNW95385.1"
                     /translation="MRVMIVGGGKVGTYLANYLINEGHEVRLIELRVEEKQRILLDVP
                     ENVIYIGNGTDPDVLEAAGIRKMDVVAAVTGQDEVNLVATSLARFEFMIPRTIARINH
                     PKNAWMFTDVMGVDVALNQADLMAALIAEEMSLGDMITLHKLRKGKFDLVEEKVAPNA
                     LANGKKLRDLDFPPQCTIVAVLRKGELVIPHGELDFQTADEVLAVVQQDQKKELARFF
                     DRIL"
     gene            complement(14157..14804)
                     /locus_tag="PKL60_04280"
     CDS             complement(14157..14804)
                     /locus_tag="PKL60_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNW95386.1"
                     /translation="MKVIIIGCGRVGAGLAKNLSIQGIDVSVIDSDPKAFELLGPTFK
                     GKTFVGIGFDQKVLKDAGIERADALAAVTASDEVNLVAARMAKMVFKVPRVSARVYEP
                     QKAKIYRRMGIQTISPVTLGIDRMISTLIYSQLNIERIIGAGQVSLVDVDVKPQLVNH
                     SIKETEIPGEIQVVAITRGGKTFIPNAATQFQKDDIVHLFVKFGSKDKVSRVFGL"
     gene            complement(14801..17674)
                     /locus_tag="PKL60_04285"
     CDS             complement(14801..17674)
                     /locus_tag="PKL60_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNW95387.1"
                     /translation="MESQTTPLSAELIQRIQELTPDQLYSELSSRPEGLDQDEAARRL
                     SAYGPNVLKEVKGKPLILKFLANFTHLMAIMLWAAGIGAFVARMPELGIAVWAVNIIN
                     GAFSFFQEFRAEKATEALKKILPSQSRVMRNGEETNVLAQDLVPGDVIFLQEGDSIPA
                     DCRLLNSSDLRVNQSTLTGESHPVNRSAEPVSVEQVTRTDIPNLIFAGTFVAAGSGKA
                     IVYATGMDTNFGRIAQITQSLKEELSPLQKEMENVTKIVTIIAVGVGALFLVIALLLV
                     HIPLIESFIFTLGMVVAFVPEGLMPTVTLALAMGTQRMAKRNALIKKLSAVETLGCTN
                     VICTDKTGTLTQNEMTVRAVWVPVSRETEPFAGREIAVSGTGYDPVGTFSENSTALDP
                     RSVGALNEVILTQAKCNTARLLPPETMEDGTKTGWKVLGDPTEAALLVLAEKAGYSRA
                     DLQNELNIAELPFDSRRKRMTVVQMQRSDSAIQRVAYVKGGIREILDVCSSIRLGGQT
                     IPLSQEWVSFIMAQNDDFARQGLRVLAAAVRTLPRELDDYQVETIETDLTFLGMVAMM
                     DPPRVDVTEAVEKCRNAGIRIIMITGDYGLTAESIARRIGIIKGEDLRVVTGVELEGM
                     SEEDLVKVVERQVIFARVAPEHKLKVVSALQKLGNVVAVTGDGVNDAPALKKADIGVA
                     MGLSGSDVAKEAADMILTDDNFGSIVNAIEEGRAVYENIKRFTTYIFTSNTPEAVPFI
                     AHALSGARIPIALNVMHVLAVDLGTDIVPALALGTEPPEAGTMNRPPRKLSDHLITKS
                     LLTRAYLILGPVQALAAMAAFYFYYWTNGYAGQWIDLPASGPIYKAATSMALASVVFT
                     QIGNMFAQRSTRRSIFKTPLFNNPLIWYGILSELLLVLAIVYVPFMQRFIGTGPFEPK
                     YWLFHLLWIPSLPLVDALRKFVQSQKEKRAQSKQILSGKGETL"
BASE COUNT         3603 a         4948 c         5518 g         3905 t
ORIGIN      
        1 cgggctgctt aattcaggcg aggtttggct ggtatattgc tccacagtat atgttcctgt
       61 tcaaaaattt ccaatgagcc tattatacaa ctttcgcgct tcgtcaatcc tcgccgcgtt
      121 cacgcgcttt caacaccaat cggatgggcg tgccaacaaa agggtactcc ttgcgaatct
      181 gattttcgag atagcgcagg tacgtaaagt gccccagggt cgggtcgttg caatagagca
      241 aaaaagtggg cggttcagaa cgtacctggg tgccgtagta gatgcggaac atgcgtccgg
      301 aacccgacga gggctggtgc atgtcctgcg cgttctgcag gatgcggttg agctgggatg
      361 tggtcagccg cacgagacgc tcttcctgca cgcgcgctac catcggcaga acctggttta
      421 cccgctgccc gctggcggct gagataaaca tgaccggcac atagggcagg aagttgagcg
      481 cctgccgcac ggttttttcg aattccaccg aagtatgagt atccttttcg atgaggtccc
      541 acttgttgac aatcaggatc gcggatttcc aagcctggtt gatgtatccg gcaatgtgtg
      601 tgtcttgcgc agtgatgccg gtaacggcgt ccactaccaa aagggctaca tcgcagcgtt
      661 cgatggcctg catggcgcgc aggacggaat acttttccac gccggggatg atggcgccgc
      721 gtttgcggat gccagcggtg tcaatcaggg taatcggcag cccttcccag ctgatttggg
      781 tatcaatcgc gtcgcgcgtc gtaccgggga tggggctgac gatggagcga ttctcgccag
      841 ccagtttgtt cagcaggctg gatttcccgg cgtttggctt gcctacgatg gcaactttga
      901 tgctgttgtc ttcctcttcg tcttcagccg gctgtggaaa atgttttacc acttcgtcca
      961 acaggtcgcc ggtgccggtg ccgtgaatgg cggaaatagg gaagggttcg cccagcccca
     1021 gggaatagaa atccagcgcg ttggcgcggg tgagctcgct gtccgccttg tttacgacca
     1081 ggataatcgg agggatgctc tgccccgcgg cggcaggtct gcggcggcgc agcatttgca
     1141 caatttcgcg gtcagcctcg gttacaccct gctgggcgtc caccaacagc aaaatcacat
     1201 cggcttcttc catcgcgatt tccgcctgga cgcggatatc agcgataaat tccgctgaac
     1261 cgatggagag cggctcctgg ctgcgctgac gggtggggtc aatcccaccg gtatcgacga
     1321 cgaaaaaatc gtgcccggac caatcgcctt ccgcgaccag gcggtcgcgc gtggtgccgg
     1381 ggacgtcgtc gataatcgcc aggcgttcgc caatcaggcg gttgaatagt gtgctttttc
     1441 caacatttgg tcttccgacc aaaacaatga cgggtttctt catgattact ttcttttcag
     1501 gttttttggc tgcggctacc tggtgatgac cacctcgatg gacgcgggca gaatggagtc
     1561 caggttaatt gcagtatctg ccagggtaac ggtcgggatt aactggtagg taccgggcgt
     1621 tttattggcc aggtccagca tgacgattat atcggccgcg ttcagcttgt ccagcaggta
     1681 gagcggtccg gagacgtaaa tatccacccg gtcgggcgaa agcgcggctt tcaagccctg
     1741 ccccaggttg cttatctcca ccgggatgcc ggtgagaagg atggaggatt ggatggccga
     1801 gatgcttaca tggatgctga cctgctggtc gccaaccacc acgatgcctt ccgggaggtt
     1861 caggttgacg tccttctgga agctctccga ggcgccattc aggttcagcg gcgtcgtctc
     1921 gataaatgcg ggcatattgg cggcaagctc cgggtctgtg ctgtaaattg taatcgtggg
     1981 cgggtccgcc aggatgccgg tgaggtagaa gccttgcgca atttgcccgg tggtaacgat
     2041 tttgacgaat acgttgcggt agccgccgag ctgcgtgacg ggaatctcga ccgtcacctg
     2101 ggtggggttc aggttaatcc cgctgacaat cacgccgcgc gcgtccaggg ctttcagctc
     2161 cacctgccgg gagatgctgg tgctgacgtt gttcaggtcc aggcccgcca ccacgcgctc
     2221 gactttcagc acctggctct cgggtccaat tgcgacggct gtgtcgtgcg aaagcacagc
     2281 ctcgccggct tcatagccaa tcggcaggct gccggtcagg cgaagttcta ccgggacctc
     2341 tttgctgagg atgttctcga gcgtgagggt aatctcgggg ggagttacca gcgtgacgcg
     2401 ggtggggctc aggtcaatgg cgatctgcgg ttccagcgtg tgttcgccgg ctgttaaacc
     2461 tgaaaggttg atgtaggcgt ggacggcatc cgggttggcc gccaggcgct cttgcacgga
     2521 acgcggcgcg cggatcctca ccttcacttc atcttccagc tgaccggtga tgaccaggtc
     2581 gggatcaagc cccagcaccg tgacagggac aggcgtcgcg tactcgcgtt cttcgttcgg
     2641 gtccgtgctg gacacggcgg atacccacac gaagatcgcc agtatcagag cggtgaacag
     2701 cgtaggcagg aatttaacaa tccaacggag ggttttcatt tcgggctccc tgcctgtttt
     2761 tcgtcttccg ttttcttttc agcggctttt gcggctttct ttccgggctg ctcatccggg
     2821 agcaggaagg cgcgcagcgt gttcagcaag cgctcgccat ccagccggcg gatgaatctg
     2881 ccctggtacg cgatggatat gctgccagtt tcttctgaga caatcagcgc gactgcgtcg
     2941 cttacttcgc tgattcccaa accagcacgg tggcgcaagc ccacggagcg ctctggcagg
     3001 ctgttcaaca cgccgctggc agaaagcggc atcacgcagg cggccgccgt tatgcggttt
     3061 ccttcgacta tgaccgcgcc gtcgtgcagg ggggtgttcg ggtaaaagat ttgcagcagc
     3121 aattcggcgc tgagatctgc gttcaggaaa acgccgttct tgtagtactc cgtcagaaaa
     3181 tcattgcggc ggataacaat caacgcgccg tggcggcgtt cagagaggcg cactgccgct
     3241 ttggtgattt cctgcagggt aatctctgtg cgttcgcgcg ccaaggggtt ggagcgacgg
     3301 gtcaggaagt cgatgttgga aaagcgcccg acccgctcaa gcgcgcgccg gatttccggc
     3361 gcgaagatga cgggaatgac aaaaatcgtt gccggggcga tggtgtttac cagccaggaa
     3421 aaagcgggca ggttcaccag cgaggtaacc agtgtgatta gaattaccac caggaagatg
     3481 ccgcgcagca gggtgagcgc ttcggtatcc cgcagcatat agagcagaat cgcaaaaaag
     3541 accgtcacaa gggcgatgtc gataacgctt atccagtcaa agcgctcaag gataaagagg
     3601 aaattgtcga ttaactcctg catgcgggca ccatcttacg gctcgtcagt aaaacctaag
     3661 tataatctgt taacatgctg cgcgctaatg tcctcgatta aaaagtagag cggacgaaga
     3721 cctcttccgg caccaggccg tttttgcgca gaaaattggc ggtggagagc ggcatcacat
     3781 ccgcgacagc ggcctcgccg ggcaggaacc ccagctgttt ggcggaggcg ggagcaatca
     3841 tctcgcgcgg gatgtagagc gctttgcaca ggtgcgcgcc ggcgcaaatc gccaggacgg
     3901 agagggcgga tttgagctct tccgagccgg gcgtgtatgg aatgacgctc tgcaccaacc
     3961 cgaggaaaaa ctcaagcttc caggaacaca gcgcgcttat ttctccttcc cgaaagccgg
     4021 ccagcagcga acggtggctg agtgagcggt aaatatcctc gtcctcccag gtctggggga
     4081 aaaagcggtt cagcagagcg cgcgccgaag gaaaagcctc cggacgcagc ggaaggaaga
     4141 gttcgccggc gcgtgagaga atctcagccg cggggggggc ttgaagcttg ttcaggccgg
     4201 ccatcgtttg cgcgtacgtt tcctgaaagc tgccgtccgt gctgatgacg taatctgagc
     4261 ggctggcttt ttcttcctgc gggctttggg agcggatgcg ctgcatcgcg tcggcttccg
     4321 tcaatccgcg cagattcatc agtcgttcct gacgcagttc atcccgggct gtgaccgtcc
     4381 aaacggcatc gcagcgctcg tgcagaccgg cttcgaagag cttaatcgct tctacgacca
     4441 ccagctccac ctccgtccgg gcaagcacag cttcaatgcc ctgcgtcacg agcgggtgca
     4501 cgatggcttc cagttcgcgc agggcgccgg ggtctttgaa gacaattttc ccgagcctgg
     4561 agcggttgat ttgtccatcc gagtagcata ggtcgctgcc gaaggcgtcc aggataggct
     4621 ggtaagccgg catgcctggc agataggtgg attgcgccag caaatcggcg tcgatggtca
     4681 gagcgccttc attttcgaga aactggcgca cggcgctctt ccctgaggca acgttcccgg
     4741 tgattccaag cagtatgggc atagcttttt gctcctcctc gttaagtata cagcaaataa
     4801 ggcagaggca gcgcaggctt aggacaggat gctataattt tcctgtgatt actgagagaa
     4861 ttttatcatc tctgcgcgag gactgccgct gggaccccgc gcgaccgctg gtggttgggg
     4921 tttccggcgg ggccgacagc ctgacgctgg cacactgcct gctgcaagcc gggggtaagt
     4981 tcacggccgc gcatctggac cacggcctgc gcccaacctc cgcggaggaa gcggagaaga
     5041 cgcgcgcgct cttggcttcc tgggggctgg cttgtcgagt ggaacggcag gacgtacggg
     5101 tttacgccag ggagcatagg ctggggatag aagaagccgc ccgccagtgc cgctaccgct
     5161 tcctcttccg cctggcggag gaaatcgggg cgcaggcggt cgcggtcggg cataccgctg
     5221 acgaccaggt ggaaaccgtg ctgatgcatt tcattcgcgg gtcaggtatt aacgggctgc
     5281 gcggaatcca ggcgcgctcc attctggcag atttccacgc ggagctgccc cttttccgcc
     5341 ccatgttggg cgtcagccga gcggaaacgg aggcctactg cagggatcac cagcttgcgt
     5401 acctcgtcga tgaaagcaat gcggatccaa gcttctttcg caaccgcctg cggcatgaaa
     5461 tcatccccgc gttggaggct gccaacccgg ctttccggcg ggttatcctg cgcaacgcgc
     5521 gcgctttgca atctgatgcc gaactgctgg cggaaatcga ggagcaggcg ctgagcaagt
     5581 gcgtgcggga acacagcgcc gaccagcttg tgctggacgc ccacgcattt gcggccatga
     5641 acgagggttt gcagcggcgc gttctgatgc aggctgtgct gcgcttgcgc cccgggctgc
     5701 gggatattgg cctggaagcg ttggaccgcg cgcttgtaag cttgcaggcc gggaaaaccc
     5761 gttttgattt ggtcggcagc ctggaagtct ggaaccagca gggggcgcta acgttggtgc
     5821 ccaagggctt tcggccggca ctgccgcagt atccgcagct tgcacacaac gaacgcttgg
     5881 cgctccgccc gggtgagacg ctcgcgttgg cgcagggctg ggggctgcgc gctgaaatcc
     5941 tgagcgccaa agcctacaaa aaactccccg cttgcgataa gcgcagcccg gaccacgcct
     6001 ggctgaaccc ggcggacctg agccttccgt tggaagtgcg gcctgccaga acgggtgagc
     6061 gctggtcacc tctgggcatg cccggcaaga cccagaaact gagcgacttt tttgtgaatc
     6121 aaaaaatccc tcagccagcc agggcgcttt ggccgctggt attctgcgag ggatctcttt
     6181 tatgggtggc ggggctgcgg attgcgcaag cctggcgttt gctgggcgat gagctggaag
     6241 tgctgcacct cagcctgcaa aagccgcaat aacgctccgg gctttactca atccagaaga
     6301 cttctttcag gatgttctga atggcgccgt aatccggctg cagtaccatc gctccttcgt
     6361 ccgtgacgaa ccccacggct tcgttcgggc ctaaagtgaa gccttgcgcg aggtcgggct
     6421 gtaagaactt gttcagcggc atgatataca gccacatgtc agagatgctc atgtcggtct
     6481 caatcacgtc cagagcggac tcgataaagg tcggcaggcg caccaggttg gccggggcgg
     6541 tgattttttg ggctatcgcg cgggcgactt cctgcgcgcg gcggttgcgg tcaaaatccg
     6601 aggagttcag gcgggcgcgc acataccaga gcgcgaaatt gctgtccatc tgatacatcc
     6661 ccggttccac tacacaccag cgttcggggg taaagtcgca ttggtcttcc atgtgctcgc
     6721 tgacctgcac gttcaagccg cccagcaagt ccaccacata cttgaaagcc gagaactcgg
     6781 caatggcata atggtcgggg cggaaaccga agttgtaagc caaggtgttc cccaacagct
     6841 cgtagccgcc cacggcatgg gccacgttca agcgctgcgc gtaatacccg gggatatcca
     6901 cccacaagtc gcgcgggagg gagaagaggt ggatttcgtt ggttttcagg ttgaaggcag
     6961 ccaggagcat cacatccgtg cggtagccgt tttggggggt gtaatccgag ccgagcagca
     7021 tgatggtttt cacatagggc ggaatttgaa gggcctggga atagccgttc aggtaagggt
     7081 cttcgacaac ttccgcgttt ttgctccaaa ggttgctgag gaaactgccc tctccggcgc
     7141 gcgtgcggtt gaggctgacc gcgccagccc aggcgagcgc gacgatgagt ataagcagga
     7201 ttaaaattac aggaagtgag gacttccgct gaggtttttg catatcagtt ttctttctat
     7261 ttggtcttgg tttgcaggca aatccttaat cctcgggcga gctcagcgtg tagccgtact
     7321 cttttgccag gctgcgcaac ttctgcgcca cctcgcgcca ttgaatcttg gaaacgccta
     7381 atttctggcg gattttatcc gtctctacgc cagcggtcaa gtcgttcaag gcgctggtcc
     7441 agcggcacat gtcgaaggaa aggtgcttat ccagcccggc ggcattgcca atgtcttcca
     7501 acagatactc caggcggcgc tgtgaccaag ggaagactgc atcctggata ttgtaaacgg
     7561 cagcgtattc ctggtaggct tgcacccatt ccggagagac cgacaccttg cgctccttgt
     7621 agcggtagcg cgggctggta tagcggatga agatgaaggg accgtccgga ccgtccagct
     7681 ccacatggtt ggttttcagg ttcaggcatt cgcctttttt catggcggtt tcgagcacca
     7741 atttaagcag caggtacggg cgcaaatcgc gcgatgcgga agtgcgcagc gcgtccgcgg
     7801 catccagggc gcgttcctgt tcgctttggg tcagaacgac cggcagcggg ctgatgaccg
     7861 tcttttgctc cagcttttcg gcggggtcca tggggatgat accgccgcgc gagagccagc
     7921 ggaaaaacga tttgatggca gtgatgcggc gggaaagggt ttttggactg cagggcacct
     7981 tgcggtcggt ttgcagccag ctcaggaagt tttccaggtc tttggtgctg accgcgccaa
     8041 tctgggcatc ggtgggtaaa aagccggcca aaagctgcag gtcggcggtg aaagctttga
     8101 gggtatgggg cgaacgtccc tgatcatgca ggtagatttt ccaggcctgg atggcaggct
     8161 gcatcagggt ggcagctgtc agatgagcgc tgaggttatc agaagtcgga ttcatgattg
     8221 ctccatcgaa tttcgcgtag atgattcaca ctttagtgta tacgcattcc cggcgtctgt
     8281 caaacaagtt tggagggcgt tgaaaaacaa aacttgcctt tcaggatgat tcattttggc
     8341 agcctcaatc tcaagaacgc gccgcgggta gcccagcggc aagcggttgt ttgtgacgcg
     8401 atcaaagcgc cgggaaagcg aaaaatgttc gataaagggc gcggcctgct ctcaatgatc
     8461 aactgccagt cccagcggta gttgatccgc ttcatagcat ccatttcgtc cgctgcgaaa
     8521 ggggcaagcg gggaaaggct gttcgcaggt tcgttcgtca aaggaactgg tctctttgga
     8581 aaactaccag ggtttactaa gtgccttcct gagcctttat ccacaccctg ctcagtcgtt
     8641 cagatacaaa gcttcggcaa tgattggatt gatggcgtag taattgggaa tcagcaccat
     8701 ggcgccttcc gccgtaatcc aattggtcac cgtttccggt cctatggcat actgattgat
     8761 gttcattgtc tgcatcacct cagaagccag cgtcatataa ggtaaaatat cggccaactg
     8821 cagattcgta tccacgtatt cgcggtaggc gccgtagagc tcgggcagct tgaaaaccac
     8881 attcaggttc attgccttgc ggaaaatagc tttcaccaca tcctgggcgc ggcggttgcg
     8941 gtcaaagtcg ctggtggtca agcgcgaacg cgagtaccac agcgcggtgc cgccgtccat
     9001 gtgggtcatg cctggttcca tgatgcacca gccgctgggg tccagcttgc aagagtcctg
     9061 cagttcctgg gtcacttcca cgtcaatgcc gtccaggctg ttgaccacgt ccttgaagcc
     9121 gttgtaattg accatcatgt aaaaatccgg gtgaatgccg aaattcgcct ggaaggtatc
     9181 cgcaaggagc tcaaagccgc ccagacccca ggcggtgttg atgcggttgg accagtagcc
     9241 cgggatggtg acgtacaggt cgcgcgggaa ggaaatgagg gagatggaat ggtgcctggg
     9301 gttgagcgag agcagcatga tcacatctgt gcggaagccg ttggcggaaa agtcgtcgca
     9361 gcccagaagc aggatgttca gttggttttc gggcagtcga tcctgggaga tgaccggcac
     9421 gggcgtgggg gtatagatgg gttggggcag caattcaatc gtgccgtcct ccaggatgtt
     9481 ggcaaaggcg ttgggttgga aaggcgttgg cgtcgcgcta aaatccttaa agctgatgcg
     9541 atacgggaat gcgcttgaat ttgcggctgc gctcaccagc gcgcctgaga ccaaaaccag
     9601 cacgaatgcc agcgcgatgg ctccgaacac gataagggtg atcatctgat gtctttttag
     9661 tctcatcgtt tccaactcct ggcgcggcgc gcccggtcta ctccgtttgc ggcagccctt
     9721 gcagggcgcc atccagaggg attagcgcgc gccaaagggt ctgttggtcg gtttcgcttt
     9781 tcagccacat gtgcccgccg catgcgctca gctgccgtgc cgctgcgccg agcgcattaa
     9841 aatcgccaat acctgcagat gcttcagcgt gttcgcccag gagcgcttca ggcaagtgct
     9901 gctgttcctg cgggctcagg ctttggccgg catgcgccgc caatatttcc acagcggggc
     9961 gtccgtcttc caggcgcgcg ggggcaaggg agaacgcgac ttcgctttcc ggcggtgaag
    10021 ccgcaaaagc gttaatgacc aggttctcaa agacctgcgc aacacaagcg agcgctgctg
    10081 gtccaagctc cggcagcgcg ccaaaagtaa gctggtgcgc gacccgccgg gaatagaagg
    10141 acgcggccag ctcatccagt ttgttttgca aggcggcgcg ggttgggcta ttctcagggg
    10201 catgttcttc ggcaccggcg gcggactggt cggaccgggc tggcgctgat gtttccgtgc
    10261 ccgccggcgc ggaacccgcc ttgcccagca ggtcgcccag cgcagcgcgg gcattttcaa
    10321 gttcgcgacg caggctgagc gcgcgttgtt cgttttcctg taaggagcgc tcgagggctg
    10381 atttttgctc ccgcagcgcg tccagctccg aaagggcgct ctgttgggct tgcagctttt
    10441 catgcagcgc ttccagttgg gcttccaaat atttctgccg ctgctcgcgc acgcgcagct
    10501 cggctgtata agcggcctgg tcgttcgcgc gctgcgaaat gagcgcggca agttccgctt
    10561 cccgggtgga aagggaatcc gccaggtcgc gtttttcgcg ttccatttga ttggcgaggt
    10621 agcggctttc gtcctttaac gcgctgaatg cttccacggc tgccgtgcgc tccaacacct
    10681 ggctcaactt atccgagagc gggctgagag cgtccaaatc cgcgctcgtc caggcccgtt
    10741 gggtgtacgg gttgaaacac aacaatgccc ggctgccaaa tgggaacagc agcaggggtc
    10801 cgcaagtatt aaaaccgctg gcgcgcatga gcgcttgttt ttcgccagcc agctgttcat
    10861 ccgcgttggc aatcagcggc accaggtctt caaaatggcg tctgagtttg ggcgcctggg
    10921 aaaagggcag cgaaaaagcc ggaagagttg cctcgctgat gaggtcgtag gcgctgccta
    10981 cgcttaaagc aggggactgc gggtcttttt ccaaaattcc gaccacatcg cctatcaggt
    11041 agagactcag gctgtgggtg aaagaggcgc ggatttcctc cgcgcgcgag agcagaccga
    11101 cgtccagcag ggcggaggcc atgcggggcg agctgacggc cgcggcgcgg gcttgttcgc
    11161 cgcggcggaa ttgccaggcg gcactgatga gcaaggggta gtagagcagt tggctgagca
    11221 tcacgccgct gggcagcgcg ctctccaggg ggaagaggat gttcaccagg tagcctaaag
    11281 ccgcggtaag cagaatcagg acgcgttcca gcttgtgggg ccgctggctg cgcaacgcca
    11341 aaaccagagc gacaaaaagc accaacagcg cggcagccga ccaggccaga ctgaagaccg
    11401 aacccgaaaa tgctttgtcg gccggccagg tagaccaggc taacacgctc agcgcgcccg
    11461 ccagtacagt gagcccgctg aggatgagcg gcaggcgcgt ctgacgcgcg ttcagccggt
    11521 cgctgagcaa gccagcgctg agccagatga gggtgagcag ggtgagcgcg tggaagacca
    11581 gtggaaaata gcggacgagc ccgtcaaaac cctgaaagat gagcaaactg agcgtcagga
    11641 gcagcacctg cgcgataaaa atcatgaaaa gggccgagcg cagagcgggg gccggctggc
    11701 ttccggaggc gctggctttg cgcagcgcta aaacagcgag cagactggcg cacagcagaa
    11761 tcaggaaata gagcagtgcg tagggaaaga ctgtgagaag aggaaggttg tccaggagag
    11821 ctttcatcag caggcgccag cttcactttg attagagctg aggttgcaag aactgttcct
    11881 gccgccaggc ggctggcgcg gcgctcaact ttccagcttc tcgagcccga tggtcagccg
    11941 gcgcagggtc tcctgcctgc ccagcaaatc cgccagctcg gtggcgccgc ccggagaggc
    12001 cggcagtccg gagagggctg tgcggatggg ccacatgacc gtgccgattt tgtagccttg
    12061 cgcggaagca aattccttga gagcggcgaa caggctcccg ttatcccagc tttccagtgt
    12121 gccaagcagc gggataaccg cgctcagcac ttgcgcgctg gaagcgagcg tggctttgga
    12181 tttctggttt tcgaacaatt ccacgctgta ttccggcagc tcgaccagga agctggtcat
    12241 cgcgggaatg tcggtcagtt tttcgacgcg aatctgaatc agccggctga ttttttcggt
    12301 atccagcttt tggatttccg cggcatagta acggctggca gcctgatgga aagcagcggg
    12361 gtccatggcg cggatgtgtt cgccgttcag ccaggtgagt ttttccatag aaaacgccgc
    12421 gctggacttg ccaatgcggt ccatgtcgaa ggcttcaatc aaatcctgca tggtaaagaa
    12481 ttcgcggtcg ttgcccgggc tccaacccaa cagggcgacg tagttcacaa tcgcttcggg
    12541 caggtagccc atgttgacca ggtcttcaaa cgaagggtcg ccggcgcgtt tgctcagctt
    12601 ggcgccgtcc tcgcgcagga tgaggggcag atggatgtat tcaggaattt gccagccaaa
    12661 agcctggtac agcaggttgt atttgggggt ggaggaaagg tattcctgcc cgcggatgac
    12721 gtgcgaaatt tccatcaggt ggtcatcgac cacattggcg aagttgtaag tcgggaagcc
    12781 gtccgatttg agcagcacct ggtcgtccag ctgcgcgttt tcgatgctga tgcggccgta
    12841 gacgtggtcg tcaaagctgg tgctgccgcc ttcgggaatg cgctgacgga ttacgggcgt
    12901 gagcccgcgg gcaaagcgtg cctgcacttc agcttgcgtc aacaggcggc aggggtcgcg
    12961 cgtgatatcc ggatgggctg gctggtcttc cggctcgggc ttgtcatctt tctggcagaa
    13021 gcagtaatac gccgcgcctc tggtaatcag ttcttccgcg tactttctgt agattggcag
    13081 gcgctggctc tggacgtatg gtcccttgcc gccgtccttg tccgggcctt cgtcgtaggt
    13141 gatgccggcc agggccagcc cgttgtaaat cgcggcgagc gcggcgggca cgttgcggga
    13201 ttggtcggtg tcttcgatgc gcaggatgaa cacgccctga tgtttttttg ccagcagata
    13261 ggcgtacaag gcggtacgta ggttgccgat atgcatgtag ccggtcgggc tgggagcaaa
    13321 acgtgttctg aacataggtc atcctgcctg aatcttaaaa ttgactgcca atatcctacc
    13381 ataaacccgc gcgcggatac agcctgccgg gctggacaca aaaaatcccg ccgggagggc
    13441 gggattttat gaattttggg ttttcgttta gaggatgcgg tcgaagaagc gcgccagctc
    13501 ttttttctgg tcttgttgca ccactgccag cacttcatcc gcggtctgga agtccagctc
    13561 gccgtgcggg atcactaact cccctttgcg cagcacggcg acaatcgtgc attgcggcgg
    13621 aaaatccagg tcgcgcagtt ttttgccgtt ggcaagcgcg ttcggagcga ctttctcctc
    13681 cacgaggtca aatttgccct tgcgcagctt atgcagtgta atcatatcgc ccaaagacat
    13741 ttcctcggcg atcaaggctg ccatcaggtc agcctggttc agcgccacgt ccacgcccat
    13801 tacgtcagtg aacatccagg cgtttttggg atggttgatg cgcgcgattg tgcgcggaat
    13861 catgaattcg aagcgcgcca ggctggtagc gaccaaattc acctcatcct gcccggtcac
    13921 ggccgcaaca acgtccattt tccggatgcc ggcagcttcc aggacgtccg ggtcggtgcc
    13981 gtttccgatg taaatcacgt tttcaggaac atccaaaaga atgcgctgtt tctcttccac
    14041 gcgcagctca atcagacgaa cttcgtgccc ttcattgatc agataattcg ccaggtaagt
    14101 gccaacttta ccaccaccaa cgatcattac tctcatcttg tcctccttac ctttgcctac
    14161 aacccgaaga cgcggctgac tttgtccttg gagccgaatt tcacgaacag gtgcacgatg
    14221 tcgtcctttt gaaactgggt ggcggcgttc gggatgaagg tcttcccgcc gcgtgtaatc
    14281 gcgaccactt gaatttcgcc ggggatttcc gtctctttga tggagtggtt aaccaactgc
    14341 ggtttgacgt ccacatcgac caggctcacc tgacccgcgc ctatgatgcg ttcgatgttg
    14401 agctgcgaat aaatcagcgt gctgatcatc ctgtcaatac ccagcgtgac cggtgaaatg
    14461 gtttgaatac ccatgcggcg gtaaattttt gctttttgag gttcgtacac acgcgcggaa
    14521 acccgcggaa ccttgaagac catcttggcc atgcgcgcag ctacgaggtt gacctcatcc
    14581 gaagcggtca cggctgccag cgcgtccgcg cgctcgatgc cggcatcctt tagcactttc
    14641 tggtcaaaac caattccgac aaaggtcttg cccttgaaag ttggtccgag cagctcaaag
    14701 gccttggggt cgctgtcaat tacagacaca tcgatgccct gaatgctcag gtttttcgcg
    14761 agacccgcac cgacacgtcc acacccgatg ataatcactt tcataatgtt tctcctttgc
    14821 cgctaagaat ttgctttgat tgggcgcgtt tttctttctg cgattgcacg aacttccgga
    14881 gggcgtccac aagggggagg ctgggaatcc acagcagatg gaagagccaa tatttgggtt
    14941 cgaacggccc tgtgccgatg aagcgctgca tgaacggtac atagacaatg gcgaggacga
    15001 ggagcagttc ggacagaatg ccataccaaa tcagaggatt gttgaagagc ggggtcttga
    15061 aaattgaccg ccgtgtggag cgctgcgcga acatgttgcc gatctgggta aatacaactg
    15121 aggctagcgc catagacgtg gctgctttat aaatcgggcc gctggccgga aggtcaatcc
    15181 actgaccggc atatccattg gtccagtagt aaaagtaaaa agcagccatg gcggccagcg
    15241 cctgcaccgg tccgaggatc aggtaggcgc gggtaagcag cgacttggta atcagatggt
    15301 cgctgagctt ccgcggcggc cggttcatcg tgccagcttc cggaggttcc gtgcccaatg
    15361 ccagcgctgg tacgatatcg gttccgaggt ctacagccag cacgtgcata acgttcaggg
    15421 cgatcggtat gcgcgcgccg gagagggcgt gcgcgatgaa aggcacggct tctggcgtgt
    15481 tgctggtgaa tatataggtg gtgaagcgtt tgatattctc gtataccgcg cgcccttcct
    15541 caatcgcgtt cacgatggag ccgaaattgt catccgtcaa aatcatatcc gcggcttctt
    15601 tagcaacatc ggaaccggaa agtcccatcg caacgccaat gtccgctttc tttaaggcgg
    15661 gggcatcgtt cacgccgtcg ccagttaccg cgaccacgtt gccgagcttc tggagcgcag
    15721 aaaccacttt cagcttgtgt tcgggggcga cgcgggcaaa gattacctgc ctttccacca
    15781 ccttcaccag gtcctcttcg ctcatgcctt caagctccac gccggtaacc acgcgcaggt
    15841 cttcgccttt tatgatgcca atccggcggg cgatgctctc tgcggtgagg ccgtaatccc
    15901 cggtaatcat gatgatgcgg atgccggcgt tgcggcactt ctccaccgct tcggtaacgt
    15961 ccacgcgcgg cgggtccatc atggcaacca taccaaggaa ggtcaggtct gtctcgatcg
    16021 tttccacctg gtagtcgtcc agttcccgcg ggagagtgcg cacggcggcc gccagtacgc
    16081 gcagcccctg acgggcaaag tcgtcgttct gcgccatgat gaaactgacc cactcctggc
    16141 taagagggat ggtctggccg cccaggcgga tgctgctgca gacatccaga atttcccgga
    16201 ttccgccttt gacgtaagcc acgcgctgga tagcgctgtc ggaccgctgc atttgaacca
    16261 cggtcatgcg cttacggcgc gagtcgaagg gcagttcggc aatgttcagt tcgttctgca
    16321 agtccgcgcg ggaatacccg gctttttccg ccagaacaag caaggcagcc tcggtcgggt
    16381 ctcccagcac tttccaaccg gttttggtgc cgtcctccat cgtttcaggc gggagcaagc
    16441 gggcagtgtt gcactttgct tgggtcagga taacttcgtt aagagcgcct actgaacggg
    16501 gatccagcgc tgtgctgttt tcagaaaaag tgcccaccgg gtcgtaaccg gtgcccgaaa
    16561 ccgcgatttc gcgcccggca aagggttctg tttcacgcga gacaggcacc caaaccgcgc
    16621 gaacggtcat ttcgttctgt gtcagggtgc cggttttatc cgtgcagatg acattcgtgc
    16681 agcccagggt ctccaccgcg gaaagtttct tgattaatgc gttgcgcttg gccatgcgct
    16741 gggtgcccat cgccagcgcc agcgtcacgg taggcatcag cccttccggt acaaacgcga
    16801 ccaccatacc gagcgtgaaa ataaagcttt cgatcagcgg gatatgcacc agaagcaggg
    16861 caatgaccag gaacagggcg ccaaccccta ccgcgatgat tgtgacaatc ttggtgacgt
    16921 tttccatttc cttctgcagt gggctgagtt cttctttcag ggactgcgtt atctgggcaa
    16981 tccgaccgaa gttcgtgtcc atgccggtgg cgtaaacaat tgccttgccg gagccggcgg
    17041 ctacgaaggt gccagcaaaa atgaggttgg ggatatctgt gcgggtgact tgctcgacgc
    17101 tgacgggttc cgctgagcgg ttaaccgggt gggactcgcc ggtcagcgtg gactggttca
    17161 cgcgcaggtc ggagctgttc agcagccggc agtccgccgg gatgctgtcg ccttcctgca
    17221 ggaagatgac atctccgggc accaggtcct gcgccaggac gttggtttcc tcgccgttgc
    17281 gcatcacgcg cgactgggaa ggcaggattt tcttgagcgc ctcggtagct ttttccgcgc
    17341 ggaattcctg gaagaagctg aaagcgccgt tgatgatatt caccgcccac accgcgatac
    17401 ccaattcagg catgcgggcg acaaacgcgc caatgccggc agcccacagc atgatggcca
    17461 taagatgcgt gaagttggcc aggaatttga ggatgagcgg cttgcctttg acttccttga
    17521 gaacgttggg tccgtacgcg ctcaggcgcc gggcggcttc gtcctggtcc aggccttcag
    17581 gtctggagct gagctccgag tacagttggt caggcgttaa ttcctgaatc cgctggatta
    17641 attccgcact taacggtgtt gtttgtgatt ccatagatga ttaatgataa tactttctga
    17701 gtggatgaat gagtcaagtt tgtgttttta ccaacccgaa atcttactcg tatcgggcgg
    17761 cttgtcaaat gcttttcagc cgtccgaggc gttttttgct tcgaaacagc gcgaatttac
    17821 atcgatttaa caattcgggt cgcttcaggg ctgcaggctc tgctgaaagg cttcccattt
    17881 ttccataagc tgcggcagga agtattgttc aaagtacaga ttgccggggt cggctttccc
    17941 gcgccccacc tggtaatgcc caaccacgtt ggcg
//
LOCUS       DAOQAD010000034        17773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_3339_length_17773_cov_6.355740, whole genome shotgun sequence.
ACCESSION   DAOQAD010000034 DAOQAD010000000
VERSION     DAOQAD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 17773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17773
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3339_length_17773_cov_6.355740"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="PKL60_04290"
     CDS             complement(<1..1191)
                     /locus_tag="PKL60_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95388.1"
                     /translation="MNPKSLILLEYPKVLAKLKSYASFSASEALADALRPTSSLEKAL
                     AMQQLTREARYLLSVNNELNFSGAVDMRPLVDLTLRSVTLEALDLLAIRATLIISRNA
                     RRILEDHRADAPKMAELAEGLTDGLGLVDLITRTISERGEVLDSASEALGRIRSEMKV
                     SHARLMERLSRYINDADSARMLQEAIITQRGGRYVLPLRAEFKGRIKAIVHDQSASGA
                     TLFIEPIAVVEWNNKVRELELQERDEVLRVLHGLSMRIAGQAESLKLSVDAMAALDLA
                     LMKARYAEDLRAVEPELVPFAEKPPEFHPGSVIVLRRARHPLLDPAKVVPIDVVLDEQ
                     TFSVVLTGPNTGGKTVSLKTVGLLVLMAQSGLQIPAQSGSRLSLFRDVFADIGDEQSI
                     EQSLS"
     gene            1799..3625
                     /locus_tag="PKL60_04295"
     CDS             1799..3625
                     /locus_tag="PKL60_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HNW95389.1"
                     /translation="MRKFTLLTVLLAAALLLLPFQSTSSASALDADIQLLPATTPGWT
                     YTGEQAQSNLGYSWMPAGDVNGDRYDDLIVGAHNYDTTTHTNAGRVYLFTGSETGLDP
                     ANPDWTMDGEQINAYLGYSVAGAGDVNGDFYDDVLIGSHGYDLTDPATVDAGRAYLFY
                     GSASGLSLTPDWTFTGTQASQAAGVKVAGIGDVNGDGFDDIAIGSTGWDGETADEGIV
                     YVFYGSAAGPAATPDWTAESDQGASNFGISISAAGDVNGDGYADFMIGANNYDKGTTD
                     EGAAFAWYGSETGFGDPGHPANADWMAESNQVTLAFALFLGTAGDVNDDGYDDVVISS
                     HVHDHPTFDEGAVFLWYGSEDGINGGSSGNPDNANWIAESNQYGFAFGTVTGIPGDIN
                     HDGYDDVIVGCQLGAPGIFVYFGSESGPNEGSDGDLSNYDWLASQPNIPGLFNAWFGR
                     QAGSAGDLNGDGVDDIAASSHLYYEDAANPLYRAGKIWAYYTNAGVINGTVTYTGPGE
                     SPLIEISANIDLFGPPDAADYQFGGDPYSLRGLEDEMYYIFAVIDFDGSGGPPDPTDP
                     IAFYDPDHDGSPDPVDISSGSRVYAIDFDIHGALLYLPIVLK"
     gene            3800..5665
                     /locus_tag="PKL60_04300"
     CDS             3800..5665
                     /locus_tag="PKL60_04300"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNW95390.1"
                     /translation="MKQTIFTLFAAFSLLFSSACASTAVDTTASAQPQAQAAVLQSTS
                     AASAAAAQTGSSTTADITPLEIETHEESSDYTWSETDEVLIDLKGSEINVTGAGVTVK
                     GTTASITAPGTYRLSGSLDNGQIVVQTDIEQPVRLILDGVRISNASSAAIDIKQADKV
                     IIILPEGSVNTLSDGKTYTYASANADEPNAALFSKADLTIYGSGKLSVSGNYADAITS
                     KDGLLIRDAVIEVSAADDGIRGKDYLVMDNAAVSVEAGGDALKSDNDDPEALGAILLS
                     SSALDISAGGDGISAEGAVLINSGSFTIQTGEGSRANLSADASAKGIKGVGSVVINDG
                     SFSLDCADDALHSNGSIVVNGGSFTIDTGDDALHADAALTINGGTLDIQSSYEGLESA
                     LITVNGGETRLTASDDGINAASAAAGSAQTMGGRQGGMGGQAGNAWFYLTGGFIYVNA
                     GGDGVDTNGSASISGGTLLVDGPTDNGNGALDYMGSFSISGGVVAAAGSAGMAQSASQ
                     DSTQPSVLIAFESAQPAGTLVSLQDASGANVLAYGPSKTFQTLLFSSPALQSGGAYNV
                     LLGGSSSGAQQDGLYLGGSASGASTYAALTLSGTVNTLGTVGGFGAPGGPGGRRP"
     gene            5798..6628
                     /locus_tag="PKL60_04305"
     CDS             5798..6628
                     /locus_tag="PKL60_04305"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNW95391.1"
                     /translation="MNTQETLLLQDAGVVPERWIRTPNPALWSLGFEAAAFRGISLPE
                     TESVALLDRVDTKFVFPQNRLEYLLPQLKDDYCMLTIDGCRLQPYQNLYFDTPGFALF
                     HLQVNGRANQYKVRVRVYESTHEAFLEIKHKTPKGRTLKSRLAVDAQLPFFGANERMW
                     LAENLPAEFTDLEPVLGNNFTRLVLVDNDQTERVTLDVQLSFSAGWKTCSAGAAVIAE
                     VKQSQAGRPSRFLERMRAQRLHETGFSKYCVGVSSLYGQVKSNLMKEKLRLLDKLSGE
                     "
     gene            6632..7264
                     /locus_tag="PKL60_04310"
     CDS             6632..7264
                     /locus_tag="PKL60_04310"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNW95392.1"
                     /translation="MENLANFTLGFVLNFVVAFVIVRFIYYPRTKDKRFVFTFLAFNM
                     IIYFVLSLMVSIELGIGVGFGLFAIFSVLRYRTDPMPIREMTYLFIILALPVMNSTGM
                     LESSWPQLLIANSAVILVLWLLENEWGFRYEVSKHITYEKIELVPPSRRAEMIADLEA
                     RTGLRVKKLSVGKIDFLRDTAELQVTFDETDQNGWNSAAALGSRDGSLDE"
     gene            7385..8074
                     /locus_tag="PKL60_04315"
     CDS             7385..8074
                     /locus_tag="PKL60_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW95393.1"
                     /translation="MTRKILVVDDTRNVQLMLQEYLQNQNFSVTLAYDGREALEMTRA
                     ERPDLILLDVMMPNMDGFQFLSRLRAESEVPVIMITARQQEADVIRGFELGADDYITK
                     PFKLRELLMRIRAVMRRAAAAENGNPLLSIGDLSLNRDRHELRKGEESIELTPLEFLV
                     LEMLMEASGKVVKRQELCLRLIDHGFTGSESTLKIHIRNLRQKLQDDLAQPKYIETVF
                     GIGYRFLENDL"
     gene            8071..9471
                     /locus_tag="PKL60_04320"
     CDS             8071..9471
                     /locus_tag="PKL60_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW95394.1"
                     /translation="MKKSLKGKLILSYLAVALVTIAIVVMVTQFTSNQSLLDMFTDQQ
                     VAQLSESVSAYYTSNASLDGFFDYFLNTQFSAPPQQNPDDFNQAPGRGIPRGIAGLAD
                     ASGRAILPSFEYAIGETISPERLKEGEAVKVNGETIAYILRDPVSRYDLKPEEQRYIQ
                     RTRLAIALAALVGVGTAVLLGVLLAGRINKPVQRLTEASARMAQGELGQQVPVTSQDE
                     LGQLTRSFNQMSADLARGDSERKRLTADITHDLSTPLQIISGYIEMFEGGEVAMTPER
                     LGIIKTEIEHLRRLVGDLTTLSQAEAGGLDIQKSALAPLRLLENVYATYQPIAARQGV
                     ELILEKPTQALCPVEVDEGRMLQVLKNLVENALRYTPAGGNIRLRAEQDDKVRLIVAD
                     SGAGIETEDLPYIFERFYQADKARSANRGKMGLGLAICKALTAAQGCGIEARSAGKDQ
                     GTAIIVSIACASALES"
     gene            9508..10764
                     /gene="hutI"
                     /locus_tag="PKL60_04325"
     CDS             9508..10764
                     /gene="hutI"
                     /locus_tag="PKL60_04325"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNW95395.1"
                     /translation="MLLIHNTSFVYQLTPSAQRGSALGALNPLPKGAVLLEGERILAV
                     GAPADLLLQHPEADLLDAGGNAVLPGFVDPHTHLVWAGDRANEFEMRLQGKSYMEIMA
                     AGGGIAATLKATRAASLEQLLEETRPRAQSAFRHGTTTLEAKTGYGLDLETEYKQMEV
                     LLRLDEEGPLQIIPTYLGAHALPPEFQGDTAGYVRFLTRQALPELKSWWQSRAPGRSL
                     PFVDVFCEEGVFDLEQTRGILDAAAELGFPLKLHADEFANLGGASLAVELGAVSADHL
                     VKTSAEDIRKLAKSNTVAVSLPGTPFGLAQSEYTPAREIVAAGGLLALATDLNPGTSW
                     NESMQFIQALACRYLKLTPAEALAASTINAAAALGLADLLGSLSPGKQADLIILKVPD
                     ARMLSYRYGTNLVSTVIKKGAVYPAD"
     gene            10768..10968
                     /locus_tag="PKL60_04330"
     CDS             10768..10968
                     /locus_tag="PKL60_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95396.1"
                     /translation="MQSFFDLEFIRQFNNLLILALGALALIFILRGVFKVAWKVIKVI
                     FVILGLLLLFGWLFGYIHISLG"
     gene            complement(11052..11588)
                     /locus_tag="PKL60_04335"
     CDS             complement(11052..11588)
                     /locus_tag="PKL60_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95397.1"
                     /translation="MKAKHWLILAASLVLLAGGVFAFTRSQDVAAQSPTPESYPASVS
                     VQNFTPAGEKIQLGIRSAQASQDALTLTLTMSGVDYEEKVSNFRATGFEKLVCNPYIV
                     AKEPVSVMFQAYEVKAGNPTQVVYTYSLKGSITSELTLTMDWTIGPCAPAYDESNVKA
                     QRNPLLTTYHFEFKVPVK"
     gene            11753..12397
                     /locus_tag="PKL60_04340"
     CDS             11753..12397
                     /locus_tag="PKL60_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNW95398.1"
                     /translation="MTITIAQARSWYASADPVHDFEHVLRVYRMAEKIALAEGADLEI
                     VQAAALLHDSVGSAPGGDGQERLGHHLTSAQFAGEVLAAEGWQPERIAAVQHCIRAHR
                     FRHQGERPQTLEAKCLYDADKLDVLGAIGAARTIAFAALSGQPSYQEPSERFLREGTT
                     EPDEPHSSYHEYLFKLRKVKDQLFTPTGKAIAGHRHKFLEEFYAELQAECRGER"
     gene            12413..13201
                     /locus_tag="PKL60_04345"
     CDS             12413..13201
                     /locus_tag="PKL60_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNW95399.1"
                     /translation="MIGAVAGDIIGSVYEFHNHRSLDFPLFTSSSTFTDDSVLTFATA
                     AVLLGKGDYAAAYQDFARRYPNRGYGGNFYRWINTNDPQPYNSFGNGSAMRVSPVGFA
                     FDSLEETLAEAQRSAEVTHNHPEGIKGAQATAQVIFMSRKGASKAEIKQEISARYGYD
                     LSRPLDLIRKVNRFDETCPGSLPEAISVFLEGEDYEHTIRLAVSIGGDTDTIACITGG
                     MAQAFTGSIPAGIVRGARARLKPELLDILERFQARFPAAAPLLT"
     gene            13305..15080
                     /locus_tag="PKL60_04350"
     CDS             13305..15080
                     /locus_tag="PKL60_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNW95400.1"
                     /translation="MTLPMILLLAIIFIAIVLFALDALPADVIGIGIMTSLVITGILT
                     PEEGILGFGSETAIKTLGLLLLTTALIKTGFVQMVTRRIVQGVGDNKNRLFWIVSLGT
                     AAMGSFTSNTASTAFFLPVTLSVSKKLKQHPSKMLMSMAFAAILSSSMTAIATSTNLV
                     VSGLLTSYGLEPMGIFELTPVGIPILIVGLLYMFFIGRKMIPERELEDNNKYLPRDTR
                     YYAEVEIPKKSPWVEKEIRQLGLRDEYDISIISIERPGEGEVKPSPKTRLRAGDLLVL
                     DGGREGIFALNDHKVVRFTSTPEDELPESIKKKYGMAEVVLLPTSSLVGQTLRGLSFR
                     KRFGIQVLGIQRRGKVLRERLLNERLQVGDQMLIHGDPETIASLENGDNFRIISDTIE
                     EPKNTKKAPYALIIFVLSVGLAAANILPLAISTMIGALLIFVTRCITPAEAYKSMNWS
                     ALILIGCMLALGKAMDTSGLANYLAGQLVTWIGTGHAYLLLAGFFLLAMILTQPMSNQ
                     AAAVVVLPIAIETALQLGLNPRTFAMMIAVGAATSYITPLEPACMMVYGPGNYRFMDF
                     VKVGSLLTILIFGISMLMVPVLWPF"
     gene            complement(15159..15581)
                     /locus_tag="PKL60_04355"
     CDS             complement(15159..15581)
                     /locus_tag="PKL60_04355"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNW95401.1"
                     /translation="MKHIFLALSALLLLILPLAACKSAQPTPEGKLVVVEPAIKPTTT
                     SMQVGDTLEVRLPTIPKEGFSWRVQDLDSKILKQDGEAVYVADDSANAAGGTTTLRFK
                     ALKAGSSTLTLIFAGSVDGSPEMSAQSFGVSVEVLAGD"
     gene            15761..17389
                     /locus_tag="PKL60_04360"
     CDS             15761..17389
                     /locus_tag="PKL60_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95402.1"
                     /translation="MQHKHARKALLSTLLTLLVCTALVGSAAAAALAQDETPESGSPQ
                     INVISPYYSEEQITTPDGAQLTRSIINGPSKPLPEYQAEREASMTAIEPEGTLGNMPS
                     YNWVFGCSAVSGAMIAGYYDRTTYPNMYAGPANGGVMPLTDTSWPTWSDGYETYPNNP
                     LIASHNGVDGRIIKGSIDDYWIEYGSSANDPYITGGWTQHTWGTAIGDYMKTSQSAYG
                     NSDGSTTFYNWTSSPNKLTCADMVSNSIADRDGTYGRKLFYEARGYTVTDCFNQKTDN
                     NSGGFTLANFQAEINAGHPVLLNLAGHSIVGYGYNGSTIYIRDTWDNDPGHTYTMTWG
                     GSYSGMTLQSVSVVHLAMTVTHAQYLPVLWNNNAVVPNPFQNPGFESGHVAWTEYSSG
                     GWDLIYQAAETPVSAHGGSWLVWLGGADDETGQLSQTVSISASAPYLHYWYYVASEDA
                     CGYDFFRVKVNGSQVYIGNLCQSASTGGWVQGVLNLAGYVGAGKTVMFEVTTDGSLNS
                     NLFLDDVSMSNSSAMEEPEPVPAEYFPGSALLPK"
BASE COUNT         3834 a         5097 c         5142 g         3700 t
ORIGIN      
        1 ggaaagcgac tgttcgatgg actgctcatc gccgatatcg gcgaaaacgt cgcggaacag
       61 gctcaggcgc gagccggact gagcgggaat ctgcaacccg gactgcgcca ttagcaccaa
      121 taagccgacg gttttgagcg aaacggtttt gccgccagtg tttgggccgg tgagcaccac
      181 cgagaaggtt tgctcgtcca gaaccacgtc aatcgggacg actttggccg ggtccagcag
      241 ggggtggcgc gcgcggcgca ggacaatgac gctgccaggg tggaattcgg gcggtttctc
      301 cgcgaagggc accagttccg gctctacggc gcgcaagtct tcggcgtagc gggctttcat
      361 gagcgccagg tccagcgccg ccatggcatc cacgctcagc ttcaggcttt cagcttgccc
      421 ggcgatgcgc atgctcagcc cgtgtaaaac gcgcaggacc tcgtcgcgtt cctgcagctc
      481 cagttcgcgc accttgttgt tccattccac cacggcgatg ggctcgatga agagtgtggc
      541 gccggaggca gattggtcgt gcacgatggc tttgatgcgg cccttgaact ccgcgcgcag
      601 cggcagcacg taacggcccc cgcgctgggt gatgatggct tcctgcagca tgcgcgcgct
      661 gtcggcgtcg ttgatgtaac gggagagtct ttccatcagg cgcgcgtggc tgactttcat
      721 ctccgagcgg atgcggccca gggcctcgga ggcgctgtcc aacacctcgc cgcgctcgga
      781 aattgtgcgg gtgatcaggt ccaccaggcc caggccgtcg gtcagacctt ccgccaactc
      841 cgccattttg ggggcgtccg cgcggtggtc ttccaggatg cgccgggcgt tgcgggagat
      901 gatgagcgtt gcgcggatgg ccagcaggtc gagcgcttcc agagtcacgc tgcgcaaggt
      961 caggtccacc agcgggcgca tgtccaccgc gccggaaaag ttcagttcgt tattaaccga
     1021 gagcaggtag cgcgcctcgc gtgtgagctg ctgcatggcc agggcttttt ccaggctgct
     1081 ggtagggcgc agcgcgtccg ccagggcttc ggaagcgctg aacgaggcgt aggacttgag
     1141 cttcgccagg actttcgggt attccagcag aatgagggac ttcgggttca tgacccgaaa
     1201 agtgtatcat gattggcttt ttccgacggg gcgggtggtt tttttgtgag tcagcaggtt
     1261 aatgtcctta agcagcaaaa tcgaaatgtc ctgattcata tcaaaagcta taccatggac
     1321 caccagaatg agcagtaaag aattatccga aagagagaag ctgaacgggt agtagatcgt
     1381 cgaagcacgc aatgggaagc agccagaaat cccgggataa ccgaacggca atttgggggc
     1441 atatgaaacg ccatcgacag gaaggagaga aaggattggc gtcagggaag cgcagaaagc
     1501 caggtaaccg acgcatagac gcccggtttc aaagataagt gccgtattcc ttggcctgtg
     1561 tattgaaatt gcatcagaaa tatcctatag tatagctatc cgggcagcgt ttatattccg
     1621 acgcataaac ccgcataatt gtccaaacca atcgctaacc tgactgaatc attacgcaat
     1681 atctgaagac ctggtgcaag ccgccagcct tgcggtagga ccgtaaaagt ccaggttccc
     1741 aaggctgaca tccaggcggt tctttgaaaa catctatcca ttcctgagga ggaaatccat
     1801 gcgcaagttc actcttttga ccgtactcct ggcagcggct ctgctcctcc tgccatttca
     1861 atccacatcc tccgccagcg cgctggatgc cgatattcaa ctcctgccag ccacgacacc
     1921 cgggtggact tacaccggcg agcaggcaca gtccaatctg gggtacagct ggatgccagc
     1981 cggcgacgtg aacggcgacc gctacgacga cctgattgtc ggcgcccata actacgacac
     2041 aaccacacac accaacgcgg gacgggtgta tcttttcacg ggctccgaga ccggcctgga
     2101 ccctgccaac ccggattgga cgatggatgg agaacaaatc aatgcctacc tgggttacag
     2161 cgtggccgga gctggcgacg ttaacggcga cttctatgac gacgtgctca tcggcagcca
     2221 cggctacgac cttactgatc ctgccaccgt agatgccggc agagcctatc tgttttatgg
     2281 ctcagcctcc ggcctgtcgc tcacccctga ttggaccttc accggcacgc aagccagcca
     2341 ggcagctggg gttaaagtgg ccggcatcgg ggatgtcaac ggcgacggct ttgatgatat
     2401 tgcgattggc tccactggct gggacgggga aacagcggat gaaggcatcg tctacgtttt
     2461 ttacggttcg gctgctggtc cggctgccac tcctgattgg acggctgaat ccgaccaggg
     2521 agccagcaac ttcggcatat ccattagcgc tgccggggat gtgaacggcg atggatacgc
     2581 ggactttatg attggcgcca ataactacga caagggcacc accgacgaag gcgcggcttt
     2641 tgcctggtac ggctcggaaa ccggcttcgg cgacccgggt catccggcca atgcggattg
     2701 gatggcggaa agcaaccaag ttacacttgc ttttgcgctc ttcttgggaa ctgctgggga
     2761 tgtaaatgat gatggatatg acgatgtggt aatttcatct cacgtgcacg atcacccgac
     2821 ctttgatgaa ggtgctgtct ttctctggta cggttcggaa gacggcataa atgggggttc
     2881 atcaggaaat cctgataacg cgaattggat agccgaaagc aatcaatatg gatttgcttt
     2941 cggcacggtc accggtatcc caggggatat caatcatgat ggttatgacg atgtgattgt
     3001 tggctgccag ctgggcgcgc cgggaatttt tgtttacttc ggctcagagt ctggtccaaa
     3061 tgaaggcagt gatggtgact taagcaatta cgattggctt gccagtcaac ctaacattcc
     3121 cggactgttc aatgcgtggt tcggtcggca agctggcagc gcgggcgacc tgaacggaga
     3181 cggcgtggat gatattgcgg cgtcttccca tctgtactat gaagacgcgg ccaacccgct
     3241 ttaccgcgcc gggaaaatct gggcttatta caccaacgcc ggcgtgatta acggcaccgt
     3301 cacttataca ggacccggtg agagtccgtt gattgaaatt tccgcaaata ttgatttgtt
     3361 cggaccccct gatgcggctg attaccagtt tggtggggat ccgtactcgc taagaggcct
     3421 cgaggatgaa atgtactata tctttgcagt gattgacttc gatggctctg gcggacctcc
     3481 cgacccaact gatccgatag cgttttacga ccccgaccat gacggcagcc cggaccctgt
     3541 ggatatttct tccggcagcc gggtgtatgc cattgacttt gacatccatg gagcgctgct
     3601 gtacctgccg atcgtgctaa agtaaatcga atttcaggtc ctggacttca ggcgcggcat
     3661 ccaccgcgcc tgcgtgtgtt taaccgcctc aatcagcccg gcagccgcgc ggcgttctat
     3721 accattgata aaccagtggg attttctttc catctacact tcaactatcc aatatagaaa
     3781 aggataggat accaatacca tgaaacaaac catatttaca cttttcgcag cgttttcgct
     3841 gcttttcagc agcgcttgcg cgagcacggc agtcgataca acagccagcg cgcagcccca
     3901 ggcgcaagcg gcggtgcttc agtccacctc cgcggcatct gctgccgcgg cacaaacagg
     3961 cagttcaaca actgcagata tcacgcctct ggagattgaa acccacgaag agagcagtga
     4021 ttacacctgg tcggaaaccg atgaggtcct gattgacctt aagggctccg agatcaatgt
     4081 gaccggagct ggcgtcacgg tgaaaggcac gacggcgtcc atcaccgcgc ccggcactta
     4141 ccgcctgagc ggcagcctgg acaacggcca gattgtggtg cagactgata ttgagcagcc
     4201 cgtgcgtctg attttggacg gcgtcaggat cagcaatgcg agcagcgccg ccattgatat
     4261 caaacaggcg gataaggtca tcatcatcct tccggaaggc agcgtgaaca cccttagcga
     4321 tgggaaaacc tatacttacg cctccgcgaa cgcggacgaa cccaacgccg cgctgttttc
     4381 caaggcggac ctgaccatct acggcagcgg gaagctcagc gtcagcggca attacgccga
     4441 cgcgattact tccaaggacg gcttactcat ccgggacgcg gtcatcgagg tgagcgccgc
     4501 tgatgacggc atccgcggaa aggattacct ggtcatggat aacgcggccg tcagcgtcga
     4561 ggccggcggg gacgcgctca aatccgataa tgacgacccg gaagctttgg gcgcgatcct
     4621 gctaagcagc agcgccttgg acatcagcgc cggcggcgat ggaatcagcg cggaaggagc
     4681 ggtgctaatc aattcaggca gctttaccat ccaaaccggc gagggcagcc gcgccaacct
     4741 cagcgctgat gcctctgcca agggcatcaa gggagtgggc tcagtggtga ttaacgacgg
     4801 cagcttcagc ctggattgcg ccgatgacgc gctgcactcc aacggcagta tcgtcgtgaa
     4861 tggcggaagc tttaccatcg atacagggga tgacgccctt cacgccgacg cagccctgac
     4921 catcaacggc ggaacgctcg acatccagag cagctatgaa ggcctggaaa gcgccctgat
     4981 cacggtcaac gggggtgaaa cgcgcttgac cgccagtgat gatggcatca acgcggcctc
     5041 cgcggcggct ggcagcgccc agacgatggg cggcagacag ggcggcatgg gaggacaagc
     5101 cggcaatgcc tggttctatc tcaccggcgg tttcatctat gtcaacgctg gcggggacgg
     5161 cgtggacacc aacggttccg ccagcatctc tggcggcacg ctgctggtgg acggcccaac
     5221 cgataacggc aacggcgcgc tggattacat gggcagcttc agcatcagcg gcggcgtggt
     5281 cgcggcggcc ggcagcgccg gcatggcgca aagcgccagc caggactcta ctcagccttc
     5341 cgtgctcatt gccttcgaga gcgcccagcc agccggcacc ctggtgagct tgcaggacgc
     5401 aagcggcgcc aatgtgctcg cgtacgggcc ttccaagacc tttcagaccc tgctgttctc
     5461 cagccctgct ctgcaaagcg gcggcgctta taacgtgctc ttaggcgggt catcctctgg
     5521 cgctcagcag gacggcctgt acctgggcgg cagcgccagc ggtgcaagca cctacgcggc
     5581 gctgaccctc tccgggactg tcaacacgct tggcacagtt ggcggctttg gggcccccgg
     5641 tgggccaggc gggagacgac cctgatccct gaaacgattt gagccgaaac aggcagcccg
     5701 cgcgggctgc ctgtttctat ccgggagtat acgaaaagta aaccaaagcg aaaagcccgg
     5761 aagttatcat gggagcatga tgaacaaagg aactcgtatg aacacacagg aaacccttct
     5821 tttgcaggac gccggcgtag tgccggagag atggataaga acccccaacc cggccctatg
     5881 gagcctggga tttgaggcgg ctgcctttcg cggcatcagt ctgccggaaa cagaaagcgt
     5941 ggcattgctt gaccgcgtgg acaccaaatt tgttttcccg caaaaccgcc tggaatacct
     6001 gctgccgcag ctcaaggatg attactgcat gctgaccatc gacggctgtc ggctgcagcc
     6061 ttatcagaac ctgtatttcg acacccccgg ttttgcgctt ttccacctgc aggtgaacgg
     6121 gcgcgccaac caatataaag tgcgcgtgcg cgtctacgaa agcacgcacg aggccttttt
     6181 ggagattaag cacaaaaccc ccaaagggcg cacgctcaag agccggctgg cagtggacgc
     6241 gcagctgccc tttttcggcg cgaatgagcg catgtggctg gcggagaacc tgcccgcaga
     6301 attcacagac ctggaacccg tcctgggaaa caactttacc cgcctggtgc tggtggataa
     6361 cgaccaaacc gaacgggtga ccctggatgt gcagctcagt ttcagcgccg gctggaagac
     6421 ctgttcggcg ggggcggcgg tcatcgccga ggtgaaacaa agccaggccg ggcgcccgtc
     6481 caggttcctt gaaaggatgc gcgcgcagcg cctgcacgaa accggcttca gcaagtactg
     6541 cgtgggggtt tccagcctgt acgggcaggt caaatccaac ctgatgaagg aaaaactgcg
     6601 tctgctggat aaactttccg gagaataagc catggaaaat cttgccaact tcacgctcgg
     6661 atttgtgctc aactttgtgg tcgccttcgt catcgtacgc ttcatatatt acccgcgcac
     6721 gaaggacaaa cgctttgtct tcaccttcct ggccttcaac atgattatct acttcgtgct
     6781 cagcctgatg gtgagcatag aattagggat tggcgtgggc tttggacttt tcgcaatatt
     6841 ttcggtgctg cgctaccgca ccgacccgat gcctatccgc gaaatgacct acctgtttat
     6901 tattctggcg ctgcccgtga tgaattccac aggaatgctg gaaagctcct ggccccaact
     6961 gctgattgcc aacagcgcgg tgattttggt gctgtggctg ctggaaaacg aatggggatt
     7021 tcggtatgag gtcagcaagc acattaccta tgaaaaaatc gaactcgtgc cgccttcccg
     7081 ccgggcagaa atgattgctg acctggaagc ccgcaccggt ctgagagtga aaaaattgag
     7141 cgtgggaaag attgatttcc tgcgtgacac cgctgaactg caggttacgt ttgatgagac
     7201 agaccagaac ggctggaatt ctgctgctgc tctgggcagc agggacggca gcctggatga
     7261 ataacccgct tgcgagatgc atttttccgt aaacctcctc ctttgaatct gaggctggac
     7321 gccgaacgtc cagcctcaaa agatgaggtc ggggaaaaag acaaaaggac gctataatct
     7381 gcttatgacc cgcaaaatcc tggtggttga cgacacccgc aacgtgcagc ttatgctgca
     7441 ggaatacctg caaaaccaaa acttctctgt cacgctggcc tacgatggac gggaagcgtt
     7501 ggaaatgacg cgcgcggaac gaccagacct gattctgctg gatgtgatga tgcccaacat
     7561 ggacggattc cagttcctga gccgtttgcg ggcggaatcc gaggtgccgg tcatcatgat
     7621 taccgccagg cagcaggaag cggacgtcat ccgcggcttt gagctcggcg cggatgacta
     7681 catcaccaaa cctttcaagc tgcgcgagct gctgatgcgc atccgggcag tcatgcggcg
     7741 cgcggcggcg gccgaaaacg ggaaccctct tctcagcatc ggagacctga gcctaaaccg
     7801 cgaccggcat gagctgcgca agggagagga gagcattgag ctcaccccgc tggagttcct
     7861 ggtgctggag atgttgatgg aagccagcgg caaagtcgtc aagcggcagg aactgtgtct
     7921 gcgtctgatt gaccacggct ttaccggctc tgaatccact ctgaagattc acatccgcaa
     7981 cctgcgccag aagcttcagg atgacctggc gcagccgaag tacatcgaaa cggtgtttgg
     8041 catcggctac cgctttcttg agaacgacct atgaaaaaat ccctgaaggg caaactcatt
     8101 ctctcttacc tcgcggtcgc gctggttacc atcgcaattg tggtcatggt cactcaattc
     8161 acatccaacc aatcgctcct ggacatgttt accgaccagc aggtggcgca gctttccgaa
     8221 tctgtgagcg cttattacac cagcaacgcc agcctggacg gcttctttga ctactttctc
     8281 aacacccagt tcagcgcccc tccgcagcag aaccccgacg atttcaacca agctcccggg
     8341 cgcggcattc cgcgcggcat cgccggcctg gcggatgcca gcgggcgtgc cattttgcct
     8401 tccttcgagt acgctatcgg cgaaacgatt tcccctgagc gcttgaaaga gggcgaagcg
     8461 gtgaaagtga acggggaaac catcgcgtat attttgcgcg accctgtgtc gaggtatgac
     8521 ctgaaacctg aagaacagcg ctacatccag cgcacccggc tggccatcgc gctggccgcc
     8581 ctggtgggtg tgggtaccgc cgtgctgctg ggcgtgctgc tggctggcag gattaacaaa
     8641 cccgtccaac ggctgactga agcttcggcg cgcatggctc agggggagct tgggcagcag
     8701 gtgcctgtaa cctcgcagga tgagctgggg cagctgacgc gcagctttaa ccagatgagc
     8761 gcggacctgg cgcgcggaga ttccgagcgc aaacgcctga ccgccgatat cacccacgac
     8821 ctgagcaccc ccctgcaaat aatctcgggc tacatcgaga tgtttgaagg cggcgaagta
     8881 gcaatgacgc ccgaacgtct gggtatcatc aaaaccgaga ttgaacattt acgccgcctg
     8941 gtaggagacc tcaccacgct ctcccaagcc gaagccggcg ggctggacat tcagaagagc
     9001 gcgctcgcgc ccctgcggct gctggaaaac gtgtacgcaa cctaccaacc cattgccgca
     9061 cgccagggcg tggagcttat cctcgaaaag cccactcagg cgctttgccc ggtggaagta
     9121 gatgaagggc gcatgctgca ggtgttaaaa aacctggtgg aaaacgccct gcgctatacc
     9181 ccggcgggcg ggaacatccg cctgcgggcc gagcaggacg acaaagttcg cctgattgtc
     9241 gcggatagcg gcgccggcat tgaaacggaa gacttgccct atatctttga gcgtttttac
     9301 caggcggata aagcccgttc ggcaaaccgc ggcaaaatgg gacttggcct ggcgatttgc
     9361 aaagcgctta ccgccgcgca gggctgcggt atcgaggcgc gctccgccgg caaagaccag
     9421 ggcaccgcga taatcgtcag catcgcttgc gcatccgcgc tggaaagctg agcctccgtc
     9481 gggcgtaagt tccggtacaa tagcagcatg ctgctcattc acaacacttc ctttgtatac
     9541 caactgaccc cgtccgccca acgcggcagc gctctcgggg cgctgaaccc tttacccaaa
     9601 ggcgccgtcc tgctggaagg cgagcgcatt ctggcagtcg gcgcgcctgc cgacctgctg
     9661 cttcagcatc cggaagcaga cttgctggac gccggcggga acgccgtttt gcccggcttt
     9721 gtggacccac atacccatct ggtctgggcc ggggaccgcg ccaacgagtt tgagatgcgc
     9781 ctgcagggga aaagttacat ggaaattatg gcagccggtg ggggcatcgc ggcgacgctg
     9841 aaagccaccc gggctgccag cctggaacag ctgctggagg aaacccgccc gcgtgcgcaa
     9901 agcgcgttcc ggcacggcac cacgactttg gaagccaaga caggttacgg gctggacctg
     9961 gaaaccgaat ataagcagat ggaagtgctg ctgcggctgg atgaagaagg tccgctgcag
    10021 attatcccca cttatctggg cgcgcacgca ttgccgccgg aattccaagg ggataccgcc
    10081 gggtatgtgc gctttctcac ccggcaagcc ctgccggaac tgaaaagctg gtggcaaagc
    10141 cgggcgcccg ggcgcagcct gccttttgtg gacgtctttt gcgaggaggg cgtcttcgac
    10201 ctggagcaaa ctcgcgggat attggacgcg gcggctgagc tcggtttccc gctcaagctg
    10261 cacgccgacg agttcgcgaa cctcggcggc gccagcctgg cagtggaatt aggtgcggtc
    10321 tcggcggacc acctggtcaa gacttccgcg gaggatatcc ggaaattagc caaaagtaat
    10381 accgtggcag tgtcgctgcc cggcacgccc tttggcttag cccagagcga atatacgccg
    10441 gcgagggaga tcgttgctgc gggcgggctg ctggcgctgg ccacggacct gaacccgggc
    10501 acgagttgga atgaatccat gcagttcatt caggcgctgg cttgccgcta cctcaagctc
    10561 acgccagccg aagccttagc tgccagcacg attaacgcgg ctgcagcgct tggcctggct
    10621 gatctgctgg gctcgctcag cccgggcaag caagccgacc tgataatcct caaagttccg
    10681 gatgcccgca tgctcagcta ccgttacggc acgaatcttg tatccactgt catcaagaaa
    10741 ggcgcggttt acccggcaga ctgaagtatg cagtcatttt tcgatttgga atttatcagg
    10801 cagttcaata acctgctcat cctcgcgctg ggcgctctgg cgcttatttt tatattgcgc
    10861 ggagtcttta aggtcgcctg gaaagtcatc aaggttattt tcgtcatcct gggcttgctg
    10921 ctgctgtttg gctggctttt tggttatatt cacatcagcc tcggctgagg tggatgctct
    10981 gaaaaacgac gaaaaaggcg cggcatttgc cgcgcctttt ttggaatttc ctttatcggg
    11041 gaagctgcgc tttactttac cggaactttg aattcaaagt gatacgtagt cagcagggga
    11101 ttgcgttggg ctttgacgtt ggattcatca taagccggcg cacaagggcc aatagtccaa
    11161 tccatcgtga gagtgagctc ggaggtgatg ctgcccttca ggctgtaggt gtagacaact
    11221 tgagtgggat tgccggcttt cacctcgtac gcttggaaca ttacggaaac gggttctttt
    11281 gccacaatat aggggttgca gaccagtttt tcaaaaccgg tcgcacggaa attggaaacc
    11341 ttttcttcat aatcaacccc gctcatcgtc agggtgagcg tgagcgcatc ctggctggcc
    11401 tgagcagagc gaatgccgag ctggattttc tcaccagcag gcgtaaagtt ttgcacgctt
    11461 acgctcgcag gatagctttc aggcgtggga gattgagcgg caacatcctg cgagcgggtg
    11521 aatgcaaaca ccccgccagc aagcagcaca aggctggccg caaggattaa ccaatgtttt
    11581 gctttcattt tttcctttca attctaacca cttacaactt agccaagggc gaaaacccct
    11641 ggtactgcca gctaagaaca taagcccttt ccgctggcct gtcaagacga accgagctgg
    11701 cgtaaaaccg ccggaaactc ctttttcgac cggcaagctt tataattgcg tcatgaccat
    11761 taccatcgct caagcccgtt cctggtacgc ttccgctgac cctgtgcacg attttgagca
    11821 tgtcttacgc gtttatcgga tggccgagaa aattgctctg gccgagggcg cagacctgga
    11881 aatcgtgcag gctgccgccc tgctgcacga ttcggtcggc tccgctcccg gcggggacgg
    11941 gcaggaacgc ctggggcacc acctgacctc cgcgcagttt gccggggaag tgctcgcggc
    12001 cgaaggctgg cagccggaac gcattgcggc cgtgcagcac tgcatccggg cgcaccgctt
    12061 ccgccaccag ggcgaacgcc cgcagaccct ggaagccaaa tgcctgtacg acgcggacaa
    12121 gctggatgtt ttgggcgcga ttggcgcggc gcgcaccatc gcattcgccg cgctgtcggg
    12181 gcagccttcg taccaggaac cctcggaacg ctttttgcgc gagggcacaa cagagcccga
    12241 cgaaccgcac agttcctacc acgagtacct gttcaagctg cgcaaagtaa aagaccagct
    12301 cttcaccccg accggcaagg ctatcgccgg gcaccgccat aaatttctgg aggaatttta
    12361 cgcggagctg caggcggaat gccgggggga gcgttagcga aaggggcagg cgatgattgg
    12421 cgcagttgct ggcgatatta tcggttccgt ttatgaattt cacaaccacc gttcgttgga
    12481 ttttccactt tttaccagca gttccacgtt cacagacgac agcgtgctga cctttgccac
    12541 cgccgccgtg ctgctgggca aaggggatta cgccgccgcg taccaggact tcgcgcgcag
    12601 atatccgaac cgcggctacg gcgggaattt ttaccgttgg attaacacga atgaccccca
    12661 gccttacaac agcttcggca acggctccgc catgcgcgtc agcccggtgg gttttgcttt
    12721 tgacagcctg gaagagaccc tggcggaagc tcagcgcagc gcggaggtga cccacaatca
    12781 tccggaaggc atcaaaggcg cgcaggccac cgcccaggtg atttttatgt cccgcaaggg
    12841 cgcgtccaaa gccgaaatca aacaggaaat cagcgcacgt tacggatatg acctttcgcg
    12901 cccgctggac ctcatccgca aggtgaaccg cttcgatgag acctgcccgg gctcgctccc
    12961 cgaagccatc agcgtgttcc tggaaggcga ggactatgag cacacgatcc ggctagcggt
    13021 ctccatcggc ggggataccg acacgattgc ctgcatcacc ggcggcatgg cgcaagcctt
    13081 tactggcagc atcccggccg ggattgtgcg cggtgcacgc gcgcgcctca aacctgagct
    13141 gctcgacatc cttgagcgct tccaggcccg atttccggcc gcggcaccgc tgttgaccta
    13201 aaaaacactc atctgcggta aaatcaaagc attcgcttcc tggtctttca ggtcagggcc
    13261 gggagcaatt tcctgctcat aaaggttcaa taacaggtgc tgtaatgacg ctgcccatga
    13321 ttctgctgct tgcgattatc ttcatcgcga ttgttctgtt cgcgttggac gcgcttcccg
    13381 cggatgtgat tggcatcggc attatgactt cgctggtcat caccggcatc cttacgcccg
    13441 aggaaggcat tcttggtttt ggctccgaaa ccgccatcaa aacgctcggt ttgctgctgc
    13501 tcaccaccgc gctcatcaag accggcttcg tgcagatggt cacccgccgg attgtgcagg
    13561 gggtgggtga taataagaat cggctgttct ggattgtctc gctgggcacc gctgccatgg
    13621 gttccttcac cagcaacacc gcctccactg ccttcttcct gccagtaacc ctcagcgttt
    13681 ccaagaaact taagcagcac ccctccaaaa tgctgatgtc catggcgttt gccgccattc
    13741 tctccagctc gatgaccgcg attgccactt ctaccaacct ggtggtcagc ggtttgctta
    13801 cctcttatgg gctggaaccc atgggcatct ttgaactgac cccggtaggc atcccgattc
    13861 tgattgtcgg cttgctgtat atgttcttta tcggcagaaa aatgattccg gagcgtgagc
    13921 tggaagacaa taacaaatat cttccgcgag atacgcgcta ctacgccgag gtggaaatcc
    13981 caaaaaaatc tccctgggta gaaaaagaaa tcaggcagct cgggctgcgg gatgaatatg
    14041 acatctccat catcagcatc gaacgacctg gggaggggga agtcaagccc tcccctaaaa
    14101 ccaggctgcg cgccggcgac ctgctggtgc tggatggagg acgcgaaggc atctttgccc
    14161 tgaatgacca caaggtcgtc cgctttacca gcactcccga ggacgagctg ccggaatcaa
    14221 tcaagaaaaa atacggcatg gcggaagtgg tgttgctgcc aacctccagc ctggtcgggc
    14281 agaccttgcg gggcttgagt tttcgcaaac gtttcggcat tcaggtgctc gggatccaac
    14341 gccggggcaa ggtcttgcgc gaacggctgc tgaacgaacg cctgcaggtt ggcgaccaaa
    14401 tgctcatcca cggcgatccg gaaaccattg ccagccttga gaacggcgat aacttccgaa
    14461 tcatcagcga taccatcgaa gagcccaaaa acaccaaaaa agcgccctac gcgttgatca
    14521 ttttcgtgct cagtgtcggt ctggccgctg ccaacatcct ccccctggcg atatccacca
    14581 tgattggcgc gctgctgatt ttcgttacgc gctgcattac gcctgccgag gcgtataaga
    14641 gtatgaactg gagcgcgctc atcctgattg ggtgcatgct ggcgctggga aaagcgatgg
    14701 acacatccgg gctggcaaac taccttgccg ggcaactggt tacctggatt ggcaccgggc
    14761 atgcctacct gctgctggct ggtttcttcc tgcttgctat gattttgact caacccatgt
    14821 ccaaccaggc agcagcggtg gtagttttac caattgcgat tgaaaccgcg ctgcagttgg
    14881 ggttgaaccc gcgcaccttc gcgatgatga ttgcggtcgg ggcggctacc tcgtacatca
    14941 ccccgctgga gccggcttgc atgatggttt acggtccggg caactaccgc tttatggatt
    15001 tcgttaaagt cggttcgctg cttaccatcc tgatttttgg gatttccatg ctgatggtgc
    15061 ccgtactctg gcctttctaa cgtttggaaa caacaatcca agaggcgccg gacaaagatt
    15121 agccggcgtt tgcgttcttc cggtgtatgg gcgaaagctt aatccccggc aagcacttcc
    15181 acgctgacgc caaaactttg cgcgctcatc tccgggctgc catccaccga gccggcaaaa
    15241 atcagggtga gcgtgctgct gccggctttc agcgccttga aacgcaacgt ggtggtaccc
    15301 ccggccgcgt tcgcgctgtc atccgccaca tacaccgcct caccgtcctg cttcaggatt
    15361 ttgctgtcca ggtcttgcac acgccagctg aagccttctt tggggatggt gggcaggcgc
    15421 acttccagag tatccccgac ctgcatactt gtggttgtcg gtttgatggc gggctccact
    15481 acaaccagct tgccttctgg cgttggttgg gcgcttttgc aggcggccag aggcaggatg
    15541 aggagcagta gagcgctgag cgcaaggaaa atgtgtttca tagagaacct ttcttgaaag
    15601 ctgtgagcgg tcatttccgc tgcacctgat ttgcagatat ccctattata tactcctgct
    15661 ggtggtataa tgtgaaaatc agtacaatcc agacctttta ccatcaacat atcaacgtct
    15721 tcgttacgcc aggtttcttt tttcatgatt caggaggatt atgcaacaca aacacgcacg
    15781 aaaagctctt ctctcaacgc tattaacctt actggtatgc accgcactgg ttggaagcgc
    15841 agccgccgct gccctggcgc aggacgaaac gcccgaaagc ggcagccccc aaatcaacgt
    15901 catttcccct tattattccg aggagcagat tactactccg gacggcgccc aactcacccg
    15961 tagcatcatc aacggtccat ccaagcctct gcccgaatat caggcggagc gcgaagcctc
    16021 gatgaccgcg attgaacccg agggcaccct cgggaatatg ccgtcgtaca attgggtgtt
    16081 cggctgttcc gcggtttccg gcgcgatgat tgccgggtat tacgaccgca ccacctatcc
    16141 caatatgtac gccggacctg ccaacggcgg cgtgatgcct ctgacggata cgtcctggcc
    16201 gacctggtcc gatgggtatg agacatatcc taacaacccc ctgatagctt cccataacgg
    16261 ggtagatggc agaataatca aaggttctat cgacgactat tggatcgaat atggcagcag
    16321 cgccaacgat ccctacatca ccggcggctg gacgcagcat acctggggca ccgccatcgg
    16381 cgattacatg aaaaccagtc agtcggccta cggcaactcg gatggctcca caactttcta
    16441 caactggacg agctcaccga ataagctgac ctgcgcggat atggtctcca atagtatcgc
    16501 cgatcgggac ggcacctacg gacgcaaatt attctacgaa gcgcgcggct acacagtgac
    16561 cgactgcttt aaccagaaga cggacaacaa ttccggcgga ttcacactgg caaatttcca
    16621 ggcagaaatt aacgccgggc accccgtatt gctgaacctg gccgggcatt ctattgtggg
    16681 ctacggctat aacggctcca cgatctatat ccgcgatacc tgggataacg accccggtca
    16741 cacctatacg atgacctggg gcggcagcta cagcgggatg actttacaat cggtcagcgt
    16801 ggtgcacctg gcaatgactg taacgcacgc gcagtatctg ccggtcttgt ggaacaacaa
    16861 tgcggtggtt cccaacccct tccagaaccc gggttttgag tcggggcatg ttgcctggac
    16921 ggaatattct tccggcggct gggacctgat ttaccaggcc gcggaaacgc cggtctctgc
    16981 gcacgggggg tcctggttgg tttggttggg cggggctgat gacgaaaccg ggcaactttc
    17041 acagacggtt tccatctctg cctccgctcc ctacctgcac tattggtatt atgtcgcgtc
    17101 ggaagatgcg tgcggatacg acttcttccg ggtgaaggtt aatggcagcc aggtatacat
    17161 tggcaatctg tgccagtccg cgagtaccgg aggctgggtt cagggcgtgt tgaacctggc
    17221 gggttatgtg ggtgcgggta aaacagttat gttcgaggtc acgacggacg gcagcctgaa
    17281 cagcaacctg tttttggatg atgtttccat gtccaattcg tccgcgatgg aagaacctga
    17341 gcctgtgccg gcggaatact tcccgggctc ggcgctgctg cccaagtagc gcttgtgctt
    17401 gtggaaacgc gagaccctcc gggcagccgg ggggtctttt tgtgggggga aaattcgtca
    17461 ttgcgactgc ttgagtgcaa tagcgactga agcaatctcc ctctatgcta acgggggggg
    17521 tcgcttcgtg cctcgcgatg acgaatgtcg gccagcaggg agatcgccgc gcttcgctcg
    17581 cgaatatgac agattcgtca ttgcgaagga gcgttagcga ctgaagcaat ctccctctat
    17641 gccagcaggg agatcgcttc gtgcctcgcg atgacgaata tcggccagca gggagatcgc
    17701 cgcgcttcgc tcgcgaatat gacagattcg tcattgcgaa ggagcgttag cgactgaagc
    17761 aatctccctc tat
//
LOCUS       DAOQAD010000035        16839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_3615_length_16839_cov_5.882567, whole genome shotgun sequence.
ACCESSION   DAOQAD010000035 DAOQAD010000000
VERSION     DAOQAD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 16839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16839
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3615_length_16839_cov_5.882567"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /gene="ccsA"
                     /locus_tag="PKL60_04365"
     CDS             complement(<1..1656)
                     /gene="ccsA"
                     /locus_tag="PKL60_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNW95403.1"
                     /translation="MFYVGFLISSLALAASAAAIILVLFSLFRKQTRIPSAAVLLTKL
                     SFFGHSAALLSLMIMQVLQDYSNVYVVSVINPAMPWILKITALWGGQTGSLFFWSWIV
                     SLCAFLALTGKKHLLDNWSFLVVSLNQILFLALSLAVDNPFTRVWALEDGSLVTSLFA
                     PQASAVVEKLNNGMGLNPLLRHPGMVIHPPLLYIGYALYLVPFAVGVSTLVRGSQSEH
                     LLEKTRTWLLAAWVFLGAGIVLGSWWSYDVLGWGGYWAWDPVETASLMPWLASTALLH
                     SLLLERRKNIFQRFNLILVLLSYLLVVFSIFVTRSGLVNSVHAFGESAISLPLLVFML
                     ALLLFSVGLLAWRWKSLNSDWKFGSAFSRDALFLYTNIILIALVLICLWGLVFPMMNS
                     ALTGVEMTLHRGFYDRSTTPLFALLVVLMSVCPLVGWSAAQFRQFGRKIWIPLGIAAL
                     GLLAVFLGGVRQAWALGGIFIVILGLSSLIYLSVQDAKSVGLKRFPKLWWKQRARYGA
                     WLVHAGILLIALGIVGMGNLAASIEGTMLPGEKMPLRGYEIEFV"
     gene            complement(1677..2162)
                     /locus_tag="PKL60_04370"
     CDS             complement(1677..2162)
                     /locus_tag="PKL60_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNW95404.1"
                     /translation="MKNKKNIKLIIGIALIAAALLYLVISSTLSQSQYFMTVAEIKDG
                     QAELVGKNIRMSGVVLGDTISYDPQTLDLSFEVAQIPGDHQLIKDMGGMSVVLAQAAH
                     DPTLPKIQVHYKGPRPDLLKDEAQAIMTGALDESGVFQVSELLLKCPSKYEAELPAQS
                     K"
     gene            complement(3234..4376)
                     /locus_tag="PKL60_04375"
     CDS             complement(3234..4376)
                     /locus_tag="PKL60_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95405.1"
                     /translation="MAEPRKKGFGDLISITSDAFGASTSDLEAPYQEDTPKMVNNRQS
                     VYRVPLSMIRPDRFQARFMLPYELREDFYTQKAGWAETVQRWLRLAETDRLVRREIDE
                     LIALGDSLHDTGQIKPVTGQVVNINGRDVFQMLTGERRFWATALQSVREGHGEEPFVL
                     ALIDNQPSIEKQIAENMAYKALTPVGKARAAARIVLEANNIKPETSEDEFAYFRKIND
                     VRLSDETRDVLMKTLQLERTYFGRLMKFFELPENLLEICDRAEMPERVLREIVSYDPE
                     YWTGAVRYYAEHEGRTYQDVHAFLERAAGREKPRAVRLPADPATKSARSIRRVLLNVE
                     EIPQDDKIGSLADALIGESEKEEAARIATRLEQLTAAVQQRLASLK"
     gene            complement(4459..5289)
                     /locus_tag="PKL60_04380"
     CDS             complement(4459..5289)
                     /locus_tag="PKL60_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNW95406.1"
                     /translation="MAIISLINRKGGVGKSTISINLAAGLAIYESRKANHKPVLLIDM
                     DDQLSATITAMGGGLDDQFVPMITAENNFPTALANGYGASDTLINDCLLPRKRTEKVC
                     IFRTNKARMEGLEAALKTREDPAFQLTYFLEPIKDEYSYIIIDNPPSAGIVPMNSLVA
                     STHVLIPIEPDGLSLIGLADILRMIEGVHKNNNPDLKILGIIPSRFRMIRRQSRDVIE
                     DMERMYGHLLLPYIKDLAEISAATTSGYDVFSYCPEKSQAYRCFSNLVDAVMGKLGQA
                     "
     gene            5482..6288
                     /locus_tag="PKL60_04385"
     CDS             5482..6288
                     /locus_tag="PKL60_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW95407.1"
                     /translation="MLSTQDLLSAGLLEHPFLHYPDTRFFYPLMSEQRRVLEVASSFI
                     LDQKDPSKNLGVIAGAVGSGKSMLAMKAAAARYQTSTRASVLGLYMNTNTLTEPRHFL
                     MAVVETLGLPSARSNANRIESIFEYLDGHNDELLLVLDGPPVDQEYLTQLLSWSVEHT
                     KKIRTLIFLQDLNNTTSNIGSLNQFLGLYVPFRAPGEQEVASLLYARLRMAGAADPLA
                     LLPEQAALSIAEQAKGSLTNALTLAGTFLEEQIDARKSRLFIPDLGPRGY"
     gene            6388..8559
                     /locus_tag="PKL60_04390"
     CDS             6388..8559
                     /locus_tag="PKL60_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95408.1"
                     /translation="MNSEENAWSDIQAVLSAKQDAALNRLLDSASLSFNGSYELCAPP
                     MVYASLCRYYPLIETVVQDLTGKDFHLTMTPTVPMGTHGVLARQPGEPAPPEDSGLTL
                     EFGASDVTEAILAPESIAAFPAYLLRFVPYVGANAVLIASALRQAFYRRSRERGADQL
                     YPRPGDAVTIDVDGLLNMLGRVISRATFFRVFKSGALDWFVRRAEPIHRFVNGKIVRQ
                     ANTYTYRGLLLTPGDAQDLYRWLGEADLRSDPAGVLKQALETPRDHLLAFPYRAPDAE
                     NDDLFTTAVSVHEVLQAALGDARLSPALAGLCDRLAAHLIRPESFLAVPWYWFHRVLP
                     DLGADLGMLYLMSKNCCYIDWARGKDRNTFWVPGGLPTLQKWIRSETLPGRIPHQKQS
                     SRGRPRKQMVNPDTQYTRDWREANRELASQYLCRVATRSTSAGTDWQLQVEEVRLTAS
                     DEILKGAVYAFLLSQHTAEQRLAYQALSSNARLVQLLRSAVRLNPERICHYETLVSEG
                     ICQFETLDAEQICYFDTLADSLNCNFETLVRAEICQFDTILKILIRLKDSAVFPKNTL
                     PPDTRANQKQSSEEDKRVVGYFTDNGWDTQALLARVNPALRERILNPASQARFLTWLI
                     HGCLTSSIHSPLSFAVSRSLETPNGAGGPAERLAALPAPLLAGLLWREHQRIQAGYIG
                     RNPLAEAGVEDLEALLASAPDTKTRAQLLRRSLDALGIAEA"
     gene            9054..10106
                     /locus_tag="PKL60_04395"
     CDS             9054..10106
                     /locus_tag="PKL60_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNW95409.1"
                     /translation="MAGGAGTRLWPISRERLPKPALRLYSEKSMFQIAVERLVPLFTP
                     QQIIVVAGAAHSAVLAEQVPEIPRENFILEPEGRGTASAIALTAVHLAARDPEAVMAV
                     LTADHFIGDEAAFRNAVAAALKAAKYDHLVTLGISPEFPSTEYGYIQQGELVREIDGF
                     KVLEARRFVEKPDQERAAEMVQSGNYAWNSGMFIWKVSVILEEFARQMPELFEQITAI
                     GKTINTDAYTKTLSEIWPKIRKQTIDYGIMEGARGVVVLPVTMQWTDVGNWNSLKALM
                     PEDARGNTIKGDAVLLDCGGTLVFGGQKLIAGVGLQDLVVVDTPDALLICRKDRVQDV
                     RKIADGLKAAGRLDLI"
     gene            10339..10680
                     /locus_tag="PKL60_04400"
     CDS             10339..10680
                     /locus_tag="PKL60_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNW95410.1"
                     /translation="MQKPAKKGKRKNLPLPVILLVAAAFIGLGVLVYQGARALSGSPI
                     LKSQDDVPRLSVQEAYQAVRNGEAVLVDTRSAEQYAAQHAAGAINLPVDSLEAQLAML
                     DPDQWYITYCT"
     gene            complement(10939..12357)
                     /gene="glnA"
                     /locus_tag="PKL60_04405"
     CDS             complement(10939..12357)
                     /gene="glnA"
                     /locus_tag="PKL60_04405"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNW95411.1"
                     /translation="MFSSFKEAHDFVRAQRIELVDLLYCDLWGHLHHVTLSAREFTEE
                     VMTEGVGFDGSSVGFKHVNAGDMVLIPDLSTSFVDPFHKQATLACLCSVYEADTKELY
                     QFDPREIARRAENWMISTGIADKSLWGPEFEFYIFDRITLKNSPAYTLCQIDSGEANW
                     GNLVNANGYALPDNQAYQAAAPCDNYYELRDEIVVTLEKLGIPVKYHHHEVGGPGQSE
                     IETPLLGITQAGDAVLLVKYVVKMTAMRAGKTATFLPKPIYGLAGSSTHYHQLLTKGG
                     LNQFYDRDGLSLLSRTALQYIGGMLTHAPAVMAFTNPSPNSYRRLVPGFEAPIKAIFS
                     VANRSAAIRIPKYATAPQAVRMEFRPPDATGNAYLSMAAMLMAGLAGIRDGIDPTEAG
                     FGPIYDNIFDWSEERRATIKSLPTSVDAAMCALENGSAFLTENGIFDETLIRTWVNTK
                     RKEANQVNQRPSPFEIENYYDC"
     gene            complement(12541..13641)
                     /locus_tag="PKL60_04410"
     CDS             complement(12541..13641)
                     /locus_tag="PKL60_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNW95412.1"
                     /translation="MSAADNTKGNASASAPERLVWLDLLRITASLLIVMLNLTSVQLK
                     SLPLDSPGWQVINLSLGAARAATPLFTMVSGVLFLSLGKQRSFSQLLKGPVRKIVVIY
                     IVWSLIYAVFSLLTDPAGEGTLLGRFAALLISSHYHLWFLPVLISLYLLSPFLQLIVD
                     QGGERILGYAAVLLVLGLLGHSLVLGGDLLPMADWVSSIAEKFPVGKVLLFAGYFLLS
                     TYLLRYFSADRKVSTVIYGLGLAGALANAAVTGLASQRMGATDLRFSDPLSLLTFFTA
                     AAVFFLFKSQVSKIRFSERASSLIANLSRLTLGVFLLHPLVIYLLENAFGLSLLSFSP
                     ILSVPLLTLLVFFLSAGLIFALKKLPYLCWMA"
     gene            13846..16350
                     /locus_tag="PKL60_04415"
     CDS             13846..16350
                     /locus_tag="PKL60_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNW95413.1"
                     /translation="MQYGYFDDKAREYVITRPDTPLPWINYLGSQDFYSLISNTGGGY
                     CFYKDARLRRITRYRYNNAPLDMGGRCLYLRDDETGQRWSAGWMPMRARLDAYECRHG
                     LGYTRIRSQSAGIEASVRFFVPLDAPLEIWDLTLTNQREKPAELSLFSAVEFALWDAW
                     DDQTNFQRNFNTGEVEVPQRDAPAGSPLAGTIFHKTEYRERRNHFAFFACSEPLAGFD
                     TQREDFLGAYRGWDDPIAVERGESANSTAHGWQPIGSHHLKLRLQPGEARRIIFLLGY
                     HENDPADKFALPGSQTIKKDSVLPVLQRFLQPKSVDSAFEQLKAEWERLLSKFQVHTP
                     DPHTNRMVNIWNAYQCMVTFNMSRSASYFESGIGRGMGFRDSNQDLLGFVHLVPERSR
                     QRILELAATQLQNGGAFHQYQPLTKRGNNDVGSGFNDDPLWLVLGVSAYVKETGDTSI
                     LDEPVPYENQPGSEMPLYEHLQRSMQYTLNALGPHGLPLIGRADWNDCLNLNCFSDTP
                     GQSFQTTTNQEGKVAESVFIAGLFVLAAKDMVQLAELHRLENAGFFQQPAEFYTEKAR
                     LMEAAVWAAGWDGAWFRRAYDAFGEPVGSALNPEGQIYIEPQGMCVMAGLGVEDGKAQ
                     AALDSVAQRLATPHGIVILQPAYTRYELKLGEISTYPPGYKENAGIFCHNNPWIMIAE
                     TRIGRGDRAFDYYTRICPSAREHISEVHRCEPYVYAQMIAGKDAPSHGEAKNSWLTGT
                     ASWNFAAITQHILGIRPTFSGLCVQPVLPESWPGFQAVREFRGTRYLINVIRVGAGNA
                     CQLRVDGKEIQGTVIPVPAGTPEEIYVEVRLGTPAL"
     gene            16374..16790
                     /locus_tag="PKL60_04420"
     CDS             16374..16790
                     /locus_tag="PKL60_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95414.1"
                     /translation="MENELLQVSTYHGEGYKPLIDFNCWRVALLRYCDDMDPSVMAAL
                     ERHLETDEVFVLLEGKAALFLTGGEGSGGLSVEMMRPGLLYNVRRGAWHNAVMSRDAA
                     ILLVENRDTGRENSESCPITPAQRAVIVEAARDVLA"
BASE COUNT         3824 a         4656 c         4665 g         3694 t
ORIGIN      
        1 gacgaattcg atttcatagc cgcgcagtgg cattttttca cccgggagca tagttccctc
       61 gatgctggcg gcaagatttc ccatgccgac aattccaagc gcaatcagga ggattccagc
      121 atggaccagc caggcgccgt aacgcgcgcg ctgtttccac cacaactttg gaaatcgttt
      181 cagaccgact gatttcgcgt cctgaacact caggtagatc aaggagctta accccaggat
      241 aacgatgaaa atgccgccaa gcgcccaggc ctgccgcaca ccgcccagaa aaaccgccaa
      301 cagcccgaga gctgcaatgc ccaatggtat ccagattttc cggccgaatt gtcgaaactg
      361 ggctgcgctc caccccacca aagggcagac cgacatcagc acaaccagca gggcaaacag
      421 cggcgtggtg ctgcggtcgt agaagccgcg gtgcagcgtc atttccacgc cagtcagcgc
      481 gctgttcatc atcgggaaaa ccaaacccca cagacaaatc aacaccagtg cgataaggat
      541 gatgttggtg taaagaaaca gcgcgtcacg gctgaaagcg gaaccaaatt tccaatcgct
      601 gttcagggat ttccagcgcc aggccaaaag tccaacggaa aaaagcagta atgccaacat
      661 gaagaccagc aagggcaagg aaatggcgct ctcgccaaag gcgtgcaccg aattcaccag
      721 cccggaacgg gtgacgaaaa tgctgaagac taccaacagg taactcagca agaccaggat
      781 taggttaaag cgctggaaga tattcttgcg gcgttccagc agcaggctgt ggagcagcgc
      841 ggtgctggcg agccagggca tcagcgaggc tgtttccacc ggatcccaag cccagtaacc
      901 gccccacccc aggacatcat aagaccacca actgccgagc acgatgccgg cgccaaggaa
      961 aacccaggca gccaacagcc aggtccgggt tttttccaac agatgctcgc tttgagaacc
     1021 gcgcacaagc gtggacactc ccacagcaaa aggcaccagg tagagcgcgt aaccgatgta
     1081 cagcagcgga ggatggataa ccatcccggg gtggcgcagc agtgggttca gacccatgcc
     1141 gttattcagt ttttccacga cggcgctggc ctgcggcgcg aacaaactcg ttaccaggct
     1201 gccgtcctcc agcgcccaca cacgcgtaaa gggattatct accgcgaggg aaagagcaag
     1261 gaacaggatt tggttcaagc tgaccacaag gaaagaccag ttgtccagca ggtgcttctt
     1321 tccggtaagc gccaggaacg cgcacaacga aactatccag ctccaaaaga aaagcgaacc
     1381 ggtctgtccg ccccacagcg cggtgatttt gaggatccag ggcatggcgg gattgataac
     1441 cgagaccaca tatacgtttg aataatcctg caaaacctgc atgatcatta acgaaagcag
     1501 cgcggcactg tgcccgaaga aactcagttt tgtcagcaga acagcggcgc tggggatccg
     1561 ggtttgcttg cggaacaggc tgaaaagtac caggatgatc gcggcagcgc tggcagccaa
     1621 cgcgagcgat gatattagga aaccaacata aaacatggac acctcgaagg cttggattac
     1681 ttgctttgcg cgggtagttc agcctcgtac ttggacgggc acttgagcag cagctcactg
     1741 acctggaaca caccggattc atccaatgcg ccggtcatga tagcctgggc ctcgtccttt
     1801 aacaggtcag ggcgcggtcc tttgtaatgc acctggattt tgggcagggt cgggtcgtgc
     1861 gctgcctggg caagcacaac tgacatgcct cccatgtcct tgatgagctg atggtcgccg
     1921 ggtatttggg caacttcaaa actgaggtcg agggtctgcg gatcgtaaga tatcgtatcc
     1981 ccgaggacaa caccggacat gcggatattc ttcccgacca gctcggcctg cccgtccttg
     2041 atttccgcaa cggtcataaa gtattgggac tgcgagaggg ttgaactgat taccaggtac
     2101 agcagcgccg ccgcgataag cgcgatacca ataattagtt ttatattttt cttgtttttc
     2161 aagttatttg aacctcattc agcgaatggg tagtattgta tctgataatt tgagttatag
     2221 gagtcctgct cagcctgaaa tagcaaaata gaaatgtcct gatgtagatc aggaactatg
     2281 gaaaccccat tcgtttttac aaaagaatga tcatccgctg acacaccagg gaatagcagc
     2341 ctttcccctc ttactccact tcccagccaa cgaaaggtaa ccgattctcc tgaggcacga
     2401 tcgctgggac ggggagttga tttttgatag agggtgccga cctctatcaa acgttttagc
     2461 cttccaatga gaaaggtgga gtaagaacaa ggatagtatc gggaagtagg gggaaggtgt
     2521 ttgcatctat cgggcttgcg cccacccgat gcttcgcacc ttccccccta ccgcccccat
     2581 ccggtaaaaa gatttcaagc cgactttgtg ctcagcctta ctagcttgtt cgtgctggga
     2641 atgggtctgg gtcaggcaag cgtgaaacag tctgaaaaag aagaccgaag aggcttattt
     2701 tcgatgtaaa cccatcctga cgtcttcctc cacgaatgag gctattttga aattgtccgc
     2761 attcgggcat tcctacttct cctcgagagg agccctccct ctggaaacga atttgctgtt
     2821 acagtggtgt gtagggatgg attccggcta agttctgatt tttcccgata tctgttgtta
     2881 ctgcctggat acctaaaccc cgaatttgtc attttgagac tttagacata agggcataat
     2941 actacgcgca ttttattacc aacaggaatt tccgcctttc cgccagcggt ttcctgggat
     3001 tttcatttcc tggaagcagt ctgatgtctt catcatcttt ggccggacaa atccgaaaag
     3061 tgccggaaaa aggaccgact caggctctga taagtggaat ttctgttcgt tcttcgggcc
     3121 gggggtttca gtctacctgc aagcgggcgg tctgctccaa caggaacttt tggacacaaa
     3181 aaaaccgctc cgggaatatc ccggcgcggt caatcggaat agggttgctt tggctacttc
     3241 aggctggcaa gcctctgctg taccgccgcg gtaagctgct ccaggcgggt agcgatgcgc
     3301 gcggcttctt ctttttccga ttcgccaatc agagcatccg ccagggatcc tatcttatca
     3361 tcctgtggaa tctcttcgac attcaggagc acgcgcctga tggaccgcgc cgatttggta
     3421 gccgggtcgg ccggcaggcg caccgcgcgc ggtttctcac ggccagctgc gcgttccagg
     3481 aaggcatgca cgtcctggta tgtgcgtccc tcgtgttccg cgtagtaacg tactgctccc
     3541 gtccaatatt ccgggtcgta agagacaatc tcccgcaata cgcgttcggg catctccgcc
     3601 cggtcacaaa tctcgagcag gttctcgggc agctcaaaga acttcattag tcggccgaag
     3661 taggtgcgtt ccagctggag tgttttcatt aacacatcgc gagtctcatc gctcagccgg
     3721 acatcgttga tttttcggaa gtaagcgaac tcatcttcgg aagtttccgg tttgatgttg
     3781 ttggcttcca acacaatgcg ggcagccgcg cgggccttcc caaccggagt aagggctttg
     3841 taggccatgt tctcagcgat ttgcttttca atgctgggtt ggttgtcgat cagcgcgagg
     3901 acaaaaggct cctcgccgtg cccctcacgc accgactgca gtgcagtcgc ccagaaacgc
     3961 ctctcaccag tcagcatctg gaatacatct ctgccattga tattgaccac ctgcccggta
     4021 accggcttga tttgccctgt gtcgtgcagc gaatccccca gcgcaatcag ttcatcaatt
     4081 tcacggcgga ccaggcggtc ggtttcagcc agcctcagcc agcgctgtac cgtctcagcc
     4141 caaccagctt tttgggtata aaaatcctcg cgcagttcgt agggcagcat aaaacgcgcc
     4201 tgaaagcggt ccggccggat catggaaagc ggcacgcggt acacgctctg gcggttattg
     4261 accatcttag gcgtgtcttc ctggtagggc gcttccaagt cgctggtgct tgccccaaag
     4321 gcatcgctgg tgatgctgat caggtctcca aaaccttttt tcctgggttc tgccattgtt
     4381 cagattcctt tctgttttcg cgctttccgt actgtgccca tgggcacact taggcacgcg
     4441 ctgcgtttct aaggccggct atgcctggcc cagtttcccc atgaccgcat ccaccagatt
     4501 ggagaagcag cggtaagcct gggatttttc cggacaatac gaaaagacgt cataaccgga
     4561 cgtggtagcc gccgagattt ccgccaggtc tttgatgtac ggcaggagca ggtgcccgta
     4621 cattctttcc atgtcctcga tgacatcgcg cgattggcgg cgaatcatac ggaagcgcga
     4681 tgggatgatt cccagaattt tcaggtcggg gttgttgttt ttatgcacgc cttcgatcat
     4741 gcgcagaatg tccgccagac ctatcaggct caatccgtcc ggttcaatcg ggatcagaac
     4801 atgggtgctg gccaccagcg agttcatagg cacgataccg gccgatgggg ggttgtcgat
     4861 gatgatgtaa gaatattcat ctttgatggg ttccaggaag taagtaagct gaaaggcggg
     4921 gtcttcgcgc gttttcaacg cggcttccaa tccttccata cgcgccttgt tcgtgcggaa
     4981 gatgcacacc ttctccgtac gcttacgcgg cagcaggcag tcgttaatga gcgtatccga
     5041 agccccatat ccgtttgcca gcgctgtcgg gaagttgttt tccgctgtga tcatgggcac
     5101 aaattgatca tccaaaccgc ctcccatggc agtgatggtg gcactcaatt gatcatccat
     5161 gtcaatcagc aataccggtt tatgattggc tttgcgggac tcataaattg ccagtccggc
     5221 tgccagattg atactgatgg tggatttgcc cacaccaccc ttacggttaa tcagagatat
     5281 aattgccatc ggcgccttgc gctcctttac ctcaaaaata catttgtata cattttacat
     5341 tattttccgt cctttacaag taactctttc ttatttatca agtatttctt gtcagttacg
     5401 caaagtgggt ggataatatt aagcacttga cttaattcat tccgcatgct aacctgttta
     5461 tacataaaag gagcgccgcc tatgctttca actcaggatt tgctttctgc tggtttactg
     5521 gaacatccct ttctgcacta cccggacacc cgtttctttt acccgctgat gagcgagcag
     5581 cggcgcgtgc tggaagttgc gtccagtttt atattggacc agaaggaccc ctcaaagaac
     5641 ctgggcgtca ttgccggggc ggtaggcagc gggaaaagca tgctggcgat gaaagccgca
     5701 gccgcccgct accagaccag tacgcgcgct tcggtccttg gtctgtatat gaacaccaac
     5761 actttgacag agccgcgcca ctttctcatg gcagtcgtag aaaccttagg tctcccatcg
     5821 gcacgctcca atgcaaaccg catcgaaagc attttcgaat atcttgatgg acacaacgac
     5881 gagctcctgc tcgttttgga cggaccgccg gtggatcagg aatacctcac ccaactcctt
     5941 tcctggtctg tggaacacac caagaaaatc cgcacgttga tcttcctgca ggacctgaac
     6001 aataccacca gcaatatcgg cagtctcaac cagttcctcg gcttgtatgt cccctttcgc
     6061 gcgcccggcg aacaggaagt cgccagtctg ctgtatgccc gcttaaggat ggccggggct
     6121 gcggatccgc tggctctgct gccggaacaa gccgctctaa gcatcgctga gcaggccaaa
     6181 ggctcgctca ccaacgcgct gacgctggca ggtacttttc tggaagaaca gatcgatgcc
     6241 aggaaaagcc gcctgttcat cccggatctt ggtccgcggg gttactgatc gagcgctcat
     6301 aatggaaaca acactgagca tcttgttcgc ggtggttaag ggttttagcc ttccatgcga
     6361 ttcaggctta cagagcaaag gcttctgatg aattctgagg aaaatgcctg gtcggatatc
     6421 caggcggttc tgagtgctaa gcaagacgcg gctttaaacc gtctgcttga ttccgccagc
     6481 ttaagcttta atggcagcta cgaactttgc gcccctccta tggtctatgc cagtctctgc
     6541 cgttattatc cgctgattga aaccgtcgtt caggatttga ctggtaagga tttccatctt
     6601 acgatgaccc caactgtgcc catgggcaca catggcgttt tagcgcggca gcctggcgaa
     6661 cccgcacccc ctgaggatag tggcctgacg cttgaatttg gcgcttcgga tgtgaccgaa
     6721 gccattctcg cgcccgaatc gattgcagct tttcctgctt acctgctgcg ctttgttccc
     6781 tatgtcggcg ccaacgcagt cctcatcgcc agcgcgctca ggcaggcctt ctaccgccgt
     6841 tcgcgcgaac gcggcgcaga ccaactttat ccccgcccgg gggacgcggt gaccatcgat
     6901 gttgacgggc tgctgaacat gctgggccgg gtcatcagcc gcgcaacctt cttccgcgtt
     6961 tttaaaagcg gcgcgttgga ttggttcgtg cgccgcgctg aaccgatcca ccgctttgtg
     7021 aacgggaaga ttgtgcgcca ggcaaacacc tatacttacc gcgggctctt gttgacccct
     7081 ggtgacgcac aagatctgta ccgctggctg ggtgaggcag accttcgttc agaccctgcc
     7141 ggcgtcctca aacaagcgct tgaaaccccc cgcgaccatt tgcttgcttt cccataccgc
     7201 gccccggacg cggaaaacga tgacctgttt acgactgctg tttcggtgca cgaagttctg
     7261 caggcagctc tgggcgatgc ccgtcttagc ccggctctgg ccggcttatg cgaccgattg
     7321 gcagcacatc tcatccggcc ggaaagcttc ctggctgtgc cttggtattg gtttcatcgg
     7381 gtcttgccgg acctgggcgc ggacctgggc atgctctacc tgatgagcaa gaactgctgc
     7441 tatatcgact gggcgcgtgg gaaggaccgc aataccttct gggttccggg aggactgccc
     7501 accctgcaaa agtggattcg ctccgaaacg ctgccgggcc gcatccccca ccagaaacaa
     7561 agctctcgcg gtcgcccgcg taaacaaatg gtgaaccctg atacgcaata cacccgagat
     7621 tggcgcgaag ccaaccgcga gcttgccagc cagtacctct gccgggttgc cacccgttcg
     7681 accagcgccg gcacagattg gcagctgcag gtggaagaag tgcgtctcac cgcctctgat
     7741 gagattctca agggcgctgt gtatgctttt ctgctcagcc aacatacagc agagcagcgt
     7801 cttgcttacc aggctctctc gtccaacgcg cgcctggtgc agctcttgcg cagcgccgtg
     7861 cggctgaacc cggagcgaat ttgtcattat gagactttag tttccgaggg tatttgtcag
     7921 tttgagacct tagacgcgga gcaaatttgt tattttgata ctttagctga cagccttaat
     7981 tgtaattttg agacgctggt cagggcagaa atttgtcagt ttgacacgat attaaaaata
     8041 cttattagac tcaaagattc tgctgttttc ccaaaaaata ctctccctcc agatacccgg
     8101 gcgaaccaaa agcaatcgtc tgaagaagat aagagggtgg tgggttattt cacagacaat
     8161 ggctgggata cacaagcgct ccttgccaga gtcaatcccg ctctcaggga acgcatcctg
     8221 aaccctgctt ctcaagcccg cttcctcacc tggctgatac acggctgcct gaccagctct
     8281 atccacagcc ccttaagttt tgctgtaagc cgcagcctgg aaaccccgaa cggcgctggc
     8341 ggaccagctg aacgtttggc agcgctgccc gccccgcttc tggctgggct cctctggcgc
     8401 gaacaccagc gcatccaggc gggttatatt ggcagaaatc cgctggctga ggcaggtgtg
     8461 gaggacctcg aagcgctcct ggcttcagcg ccggacacca aaacccgcgc tcagctgctc
     8521 cgacgcagct tggatgcgtt gggcatcgct gaggcgtaaa gcctgcaagc aagagccttc
     8581 ggtcataaag aaaccctgaa gtaattcctg agtttacatt gaaaatcata cctgaggaga
     8641 gcctccctcc cgcctgcgcc tctcgacagc cgggaatcgc ctcaagccta aaattgctga
     8701 tcccatattg ttcaatggat gcgtatagga atgatttctt ttgtcctgtt cggctgatca
     8761 agagctgcca attgcgaact tattaactca cattccatct aactgcgcat agatattaaa
     8821 taacgtttta ctaatctcaa aaatgacccg ggtcaatcaa actgcttgct ttcccgccgt
     8881 tatccggagg aattcccggg aacgcttctc accgctgttc aaagcgcaat gcgcataggc
     8941 atactgttta tatcggatgc caggcaatcc aggggagggg gcttgcttcc ccgaatcggc
     9001 tataattcca gaaaatctgg acttccggag gcaaaatgaa ctacgcggta attatggctg
     9061 ggggcgcagg caccaggctt tggcctatct cgcgcgagcg tttaccgaag ccggcactgc
     9121 gtctttacag cgaaaaaagt atgtttcaga ttgctgtgga acggctcgtg ccgcttttca
     9181 cgccacaaca aatcatcgtg gttgcgggcg cggcgcactc cgccgtgctt gccgaacagg
     9241 tcccggaaat ccccagggaa aacttcatcc tggagccgga aggccgcgga accgcctcag
     9301 ccatcgcgct gacggcagtt catctggccg cgcgcgatcc agaagccgtc atggccgtgc
     9361 tcacggcaga tcactttatc ggtgacgagg cagctttccg caacgcggtg gccgccgcgc
     9421 taaaagctgc aaaatatgac cacttggtca cattagggat ttcgcctgaa ttcccttcca
     9481 cggagtacgg ctatatccaa caaggcgagc tggtacgcga gattgatgga tttaaagttc
     9541 tggaagccag gcgctttgtg gagaaaccag accaggagcg ggcggcagag atggtccagt
     9601 ccgggaatta tgcctggaac agcgggatgt ttatctggaa ggtcagtgtc attctggagg
     9661 agtttgcgcg ccagatgccc gagctctttg aacaaatcac ggcgattggc aagacgatta
     9721 acacggacgc ctacacaaaa acgctctcgg aaatctggcc gaaaatccgc aagcagacga
     9781 ttgattacgg catcatggaa ggggctcgcg gggtggtggt cctgccggtt acgatgcagt
     9841 ggacggatgt cggaaattgg aacagtctca aggcgcttat gccagaagat gcgcgaggga
     9901 ataccataaa aggcgatgcg gtcctgttgg actgcggcgg gacgcttgtt ttcggcggtc
     9961 agaagctgat tgccggggtg ggtttgcagg acctggttgt ggtggatacg ccggacgcgc
    10021 tgctaatttg ccggaaagac cgggttcagg atgtccgaaa aatcgccgat ggattgaagg
    10081 ctgcaggtcg gttggacctc atttagacaa ccaaaatccc tggaagagac tgcctgcttg
    10141 cggtctcttt ttgatgcagt ggtgacagcc tgaacaggca ggctccgaag cccagcctta
    10201 agcgctcagt tctccataga ttcctggcgg acatcatctt aggtccacac attttcaagc
    10261 acggcgggca cgccaagcgt gtacgctaac tttcttttca ctcctgggta tgtaagaatc
    10321 cacccattga ggtgagctat gcaaaaacct gccaaaaaag gaaagcgaaa gaatctcccg
    10381 ctgccagtca tcctgctggt cgcggcggca tttatcggcc tgggggtgct ggtctaccag
    10441 ggagcgcgcg cgctcagcgg cagcccgatc ctcaaatctc aggatgatgt cccccgcctc
    10501 agcgttcagg aagcctatca agctgtgcgc aacggtgaag ccgtgttggt ggacacgcgc
    10561 tccgcagagc aatatgcggc ccagcacgca gccggggcaa tcaatctgcc agtggacagc
    10621 ctggaagctc aacttgcaat gctggacccg gaccagtggt atatcaccta ctgcacctga
    10681 ccggctgaaa atacgagcgc ccgtgcggcg ttcctgatgc ttcagagtgg ttattcaaag
    10741 gcgaatccga ttaaaggcgg ctttgaagcc tggataaatg ccggctatcc aacagaacca
    10801 taaggaatta ttgatagaaa aagacaggtg ttgaaaacac aacctgtctt ttcttttttt
    10861 cagagaaggg aattttcggt ctcctggctg tcctgaatac ctgcttattc caacctataa
    10921 cttacctggc gcaattgctc agcagtcgta ataattttca atctcgaacg ggctggggcg
    10981 ctgattaacc tgatttgcct ctttgcgctt ggtattgacc caggtgcgta tcagggtttc
    11041 atcaaaaatt ccgttttccg tgagaaacgc cgagccattt tccagggcgc acatggctgc
    11101 gtccacagac gtgggtaggg acttgatcgt ggcgcggcgt tcttcagacc agtcaaaaat
    11161 attatcgtag atcggtccga atccggcttc cgtcgggtcg atcccatccc ggatgccggc
    11221 cagacctgcc atcaacatgg ctgccatgga caagtaggca ttaccggtgg cgtcaggagg
    11281 tcggaactcc atgcgcacag cctgcggagc ggtggcatat tttgggatgc ggatggcagc
    11341 gctgcgattg gcgaccgaga agatggcttt gatgggcgcc tcgaaacctg gaaccaaccg
    11401 gcgatacgag ttgggactgg ggttggtgaa agccatcacg gccggagcat gcgtcagcat
    11461 gccgccgata tactgcaaag cggtgcggct gagcaaagac aggccgtcgc ggtcgtagaa
    11521 ctggttgagg cctcctttgg tcagaagctg gtggtagtgc gtgctgctgc cagccaaacc
    11581 gtagatgggt ttgggcagga aagtcgcggt ttttccagcg cgcatggcgg tcattttaac
    11641 cacgtacttg accaaaagca ctgcatcccc cgcctgggta atgccgagca gaggggtttc
    11701 gatctccgac tgcccgggtc cgccgacttc gtgatggtgg tatttgaccg ggatgccgag
    11761 cttctccagg gtcacgacga tttcatcgcg cagttcatag tagttgtcgc acggcgccgc
    11821 ggcctggtag gcctggttat ccggcagcgc gtagccgttg gcgttcacca gattacccca
    11881 gttcgcttcc ccggagtcaa tctggcaaag cgtgtacgcg gggctgtttt tcagcgtgat
    11941 gcggtcgaat atgtaaaact cgaattccgg accccacaag gatttgtccg cgatgccggt
    12001 ggaaatcatc caattttctg cgcgccgggc gatttcgcgc gggtcgaatt ggtacagttc
    12061 cttggtatcc gcttcgtaca cgctgcacag gcaggcgagg gtggcctgct tgtggaaagg
    12121 gtccacaaag gatgtgctca ggtcagggat gagcaccata tcgccggcgt tcacatgctt
    12181 gaaccccacg ctggagccgt cgaacccgac gccttcggtc atcacctctt cggtgaactc
    12241 cctggcggac agggtgacgt gatgcaggtg gccccacagg tcgcagtaga gcaaatcgac
    12301 caattcaatg cgctgagcgc ggacaaaatc gtgtgcttct ttgaacgatg aaaacatagg
    12361 ctcctttacg caatggattc ggtgagcgcc attgcgtttg aattataaca agaagcttgc
    12421 cagcgtcggg acgcggtttg aagccgcccc gcatcgattt cgccgttctt caaaccgggt
    12481 gaggaatttg actgcaggcc tccggcagtc cggattttct ccgaatatcc tgaaaagcgc
    12541 ttacgccatc cagcacaggt acggcagctt tttgagcgca aagataagcc cggcgctgag
    12601 gaagaacacg agcagcgtca gcaagggcac tgagagaatt gggctgaagg atagcaggct
    12661 gagcccaaac gcgttttcga gaagataaat gaccaatggg tggagaagga acacgcccaa
    12721 cgtgagccgc gataggttgg cgatcaggct gcttgcgcgc tcggaaaacc ggattttgct
    12781 tacctgcgat ttgaacagaa agaacacagc cgcagccgta aagaaagtca gcagcgaaag
    12841 cgggtccgag aagcgcaaat ccgtcgcgcc cattctctgg gaagccagcc cggtcacggc
    12901 agcgttggca agcgcccccg ccagacccag gccgtagata acagtgctga ctttacgatc
    12961 tgcggaaaaa tagcgcagca ggtacgtgct caacaaaaag tagcccgcaa agagcaaaac
    13021 tttccccacc gggaacttct ccgcgatgga actgacccag tccgccatcg ggagtaaatc
    13081 cccgccaagg accaggctgt gcccgagtaa acccaaaaca agcaaaacag ccgcgtagcc
    13141 gagaatgcgt tcgccgccct gatcgacaat caactgcaga aaaggcgaaa gcagatacag
    13201 gctgatgagg accggcagga accataagtg gtaatggctg gaaatcagca gagctgcgaa
    13261 gcggcccaga agcgttccct cgccagctgg atcggtaagc aaactaaaca ccgcgtaaat
    13321 cagcgaccac acgatgtaaa tcacaacaat ctttctgaca ggacctttca ggagctgact
    13381 gaaactgcgt tgtttcccca gactgaggaa caggacgccg ctgaccatcg tgaagagcgg
    13441 tgttgccgcc cgtgctgcgc cgagggaaag gttaatgacc tgccaacctg gcgagtccag
    13501 gggcagggat ttgagctgaa cagatgtcag gttcagcatc acaatcagga gcgaggctgt
    13561 gatgcgcaat aagtccagcc agacgaggcg ctctggcgcg gaggcggaag catttccttt
    13621 tgtgttatca gcggcgctca taagtccttg tttcccaatt cgtgatttta gaggattagc
    13681 ctgattattg ccagcctgtg taaaggcatc cggggtttcg cttgggcata gccctctgtt
    13741 ccgcgctata catctcaatg ccgtttataa tttgctggga gagcccgcgc cagtagtttt
    13801 cgtcccagga acaggcactg cgcgttggct gccaaaggag aggctatgca atacggttac
    13861 tttgatgata aagcccggga atatgtaatt acccgaccgg ataccccctt gccctggatt
    13921 aattatcttg gcagccagga tttctacagt ctgatcagca atactggcgg cggttactgc
    13981 ttctacaaag acgcccgact gcgccggatc acgcgctacc gctacaacaa cgccccgctg
    14041 gatatgggcg ggcgctgcct ttacctgcgc gatgatgaaa ccggtcagcg ttggtcggcc
    14101 ggatggatgc caatgcgcgc ccgactggac gcttacgaat gccggcatgg gcttggatac
    14161 acccggattc gttctcaatc cgcgggaatt gaggcgagcg tcaggttttt tgtgccgttg
    14221 gatgccccct tggaaatctg ggaccttacg ctcacgaacc agcgtgaaaa accggctgaa
    14281 ctcagcctgt tctccgcggt ggaattcgcg ctgtgggacg cctgggatga ccagaccaat
    14341 tttcagcgca acttcaacac gggcgaagtg gaagtacccc aaagggatgc ccccgccggc
    14401 agcccgctgg ccgggaccat cttccacaag accgaatacc gcgaacggcg caaccacttc
    14461 gcgtttttcg cgtgctctga gcctctggca ggttttgata cccagcgtga ggactttctg
    14521 ggtgcctatc gcggctggga cgaccccatc gcggtcgaac gcggagaatc cgccaactca
    14581 acagcgcacg gttggcagcc gatcggctcc caccacttga agctgcgcct gcagcccggc
    14641 gaagcgcgcc gaatcatctt tctgctgggc taccacgaaa atgatccggc ggataaattc
    14701 gcgctgcccg gttcgcaaac cataaaaaaa gacagcgtcc tgccggtttt gcagcgcttc
    14761 ttgcagccca aaagcgtgga cagcgccttc gagcagctca aagctgagtg ggaacgcctg
    14821 ttgtcaaagt tccaggtgca cacgcccgac ccgcacacga accgaatggt caatatctgg
    14881 aacgcgtacc aatgcatggt taccttcaac atgagccgct cagccagcta ttttgagtcc
    14941 gggattggac gcggaatggg attcagggat tccaatcagg acctgctagg ctttgtccat
    15001 ttggtcccgg aacgttcgcg ccaacgcatc ctggaattgg cggccaccca actgcaaaac
    15061 ggcggagctt tccaccagta tcaaccgctg accaaacgcg ggaacaacga tgtcggatcc
    15121 ggcttcaatg acgacccgct ttggctggtc ttgggtgttt ccgcgtacgt gaaggaaacc
    15181 ggggatactt ccatcctgga tgagccggtg ccgtatgaaa accagccggg ttccgaaatg
    15241 ccgctctacg agcacctgca gcgctccatg cagtacaccc tgaacgcgct gggtccgcac
    15301 ggactgccgc tgatcgggcg cgcggactgg aacgactgtc tgaacctgaa ctgcttctca
    15361 gacactccag gtcaatcctt ccagacgacc acgaaccagg agggcaaggt tgccgagagc
    15421 gtgtttattg ccgggttgtt cgtcctcgcc gccaaggata tggtgcagct ggctgaactg
    15481 caccgcctgg agaacgccgg ctttttccag cagcccgcgg agttctatac cgagaaggcg
    15541 cgcctgatgg aagccgcagt gtgggctgcc ggttgggatg gagcctggtt ccgccgcgcg
    15601 tatgatgcct tcggcgagcc ggtggggtcg gcgctgaatc ctgaaggtca gatctacata
    15661 gaacctcagg ggatgtgcgt gatggcgggt ttgggcgtgg aggacggcaa ggcccaagcc
    15721 gcgcttgatt ccgtggcgca gcgcctcgca acgccgcacg gcatcgtcat tttacagccg
    15781 gcatataccc gttatgaact gaaattaggg gaaatatcca catatccccc tggatacaag
    15841 gaaaacgccg gaattttttg ccacaacaac ccctggatta tgattgccga aaccaggatt
    15901 ggcagagggg accgcgcttt tgattattac acccgtattt gcccctctgc ccgtgaacac
    15961 atcagcgaag tgcaccgctg cgagccgtac gtctacgccc agatgattgc cggtaaagac
    16021 gcacccagcc acggggaagc caaaaattcc tggcttaccg gcaccgcatc ctggaacttc
    16081 gcggcaatca cacagcacat cctgggcatc cggccgacct tcagcggctt gtgcgtgcag
    16141 ccggtgctcc cggaaagctg gccgggcttt caggcggtgc gcgagttccg tgggacgcgt
    16201 tatctgatca acgtaatccg cgtgggcgct gggaacgcct gtcagctgcg ggtggacggc
    16261 aaggaaatcc agggcacggt cattccggtg cctgccggaa cgcctgaaga aatttacgtt
    16321 gaggtcaggc tgggcacccc agcgctgtag ttttttcaga gaagggaaaa cgcatggaaa
    16381 atgaattgct gcaggtgagt acttaccacg gcgagggcta taaaccgctg attgatttta
    16441 actgctggcg ggtggcattg ctgcgctatt gtgatgatat ggaccccagc gtgatggcgg
    16501 cgctggaacg acacctggaa acggatgaag tctttgtgct gctggaaggg aaagcggcgc
    16561 tctttctgac cggtggcgag ggttcagggg ggctgagcgt ggaaatgatg cgacctggac
    16621 tgctctataa cgtccggcgg ggggcctggc ataacgcggt aatgagccgg gacgcggcga
    16681 ttttgctcgt tgaaaatcgg gacaccggcc gggaaaattc ggaatcctgc ccgataacgc
    16741 cagcacagcg ggcagtgatt gtcgaagccg ccagagatgt tcttgcttag ctgcgcatag
    16801 cctttttgaa ttagtcacca gacgagtatt catgagggc
//
LOCUS       DAOQAD010000036        15757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_3994_length_15757_cov_7.100943, whole genome shotgun sequence.
ACCESSION   DAOQAD010000036 DAOQAD010000000
VERSION     DAOQAD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15757
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3994_length_15757_cov_7.100943"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKL60_04425"
     CDS             <1..352
                     /locus_tag="PKL60_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95415.1"
                     /translation="GQRLVIRTGVQPPLTPTPLGAQPTSLTPLPLPSATPTRTPTRTP
                     IPSPTPTATPQSPLAAFWRGNLSGALLTGALGMLLGAGLTLLLRRRAPKAPPAQEPAR
                     ETSAAKEPPQGDPE"
     gene            349..885
                     /locus_tag="PKL60_04430"
     CDS             349..885
                     /locus_tag="PKL60_04430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNW95416.1"
                     /translation="MSSTPQPPSPQRAALLVIDVQQAMFAQSTPVYQSETLLTNILSL
                     TAKARKAGAPVVWVQHCDARDLVPDTPGWQLHPALVPGAQDLKLRKQKGNAFEGTNLK
                     ELLAERGVSSVVVCGMVTHGCVKNTCLGALAEGLRVTLAQDAHSSYSKDAAALIEEWN
                     TALAQAGARVLNSGEVCF"
     gene            930..1490
                     /locus_tag="PKL60_04435"
     CDS             930..1490
                     /locus_tag="PKL60_04435"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HNW95417.1"
                     /translation="MTLRSPQDRMAAVSESIQERTGRTLEKWVQLALANGPNPLDQNS
                     VCKWLKSEHGVAQNTQWAIAEAAELAAGWQRPSEEEYALNHYSGEPVFERLRALLLGL
                     GPDVRMEGRGTYTPFVRKRQFAAVAAASRERVDLGLRFKSAPESALLSQAKAPGQATH
                     KLSLCSVEEITPEVEALLRAAYEQNG"
     gene            1509..1790
                     /locus_tag="PKL60_04440"
     CDS             1509..1790
                     /locus_tag="PKL60_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95418.1"
                     /translation="MKKFILRVLLGYGLLAFLLVASFCVIGYRAAGWQGARNAAGTGL
                     LVSAIGLPMAGLLIALKFWGGYANRWGASNYKKELEGEPKDREHDPERW"
     gene            1854..2141
                     /locus_tag="PKL60_04445"
     CDS             1854..2141
                     /locus_tag="PKL60_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95419.1"
                     /translation="MQPPKKPSALKIVLIVLLIYGIIAVMILLLSSLIGFWLNGSEGA
                     SFALGIGMMMNLLAFPAAMYILVARLRGISDTARTPKAAGSNEKSDEERSA"
     gene            2158..3354
                     /locus_tag="PKL60_04450"
     CDS             2158..3354
                     /locus_tag="PKL60_04450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW95420.1"
                     /translation="MKANHIPETRTRKTWLIYVLLSLYVFMLNVPGPITNYLKDEFAM
                     SYTLSSLHFSAFAAGVLVTGLLGAYFIPRFPRWKVMAFGALGLGVGGIILAFGQAPAV
                     TILGIFLMGCIGTLILSIYPAILDEEMGARSPVGISEANTIASLFAGIAPLLVGFLVQ
                     KAVGWRPAILLVAAASLALGLWLLLRINGSPAGGLAQKVVPEQNGKLPGRYWIFWLAL
                     MLGIAVEYCIIYWGADFVEKVLGFSKNSATQSVSLFMAGMIIGRFFGSRLLERVPPMK
                     LLTGSILLSIVGFALFWSGITPLLGIVGLTLLGLGGANFYPVIVSLAIASAGELKAKA
                     GARATLATGTAILFLPLVLGIVSDRIGIRLAYSIVAVLFVLLSLTLWWANSRQTEPAA
                     QAETAD"
     gene            3412..4404
                     /locus_tag="PKL60_04455"
     CDS             3412..4404
                     /locus_tag="PKL60_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW95421.1"
                     /translation="MKALFIGGTGNISSACSKLALERGVELYLLNRGQSFRPLPQGAR
                     VINADIRDPNAAQDALGSLKFDVVADFIAFTPQHIETDLQLFRGRAGQYLFISSASAY
                     QTPPATLPVTESTVLDNPVWQYSRDKAACEERLVRAYREEKFPFTIVRPSHTYSEVYV
                     PVHGNWTTIDRMLRGLPVIVHGDGTSLWTLTHSSDFAKGFVGLLGSSRAIGEAYHITS
                     DEWLTWNQIHEILAAAAGTRANLVHVPSDLIAAYDPIWGESLLGDKAHSFILDNSKIK
                     RVVPDFVCSTPFSRGAEEILAWHRADPARQKVDENFNAICERILTAYAKAWPEK"
     gene            4707..7268
                     /locus_tag="PKL60_04460"
     CDS             4707..7268
                     /locus_tag="PKL60_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNW95422.1"
                     /translation="MAGINRFTQKAKRVLAHAQTEAERMHKQTIGTEHLLLGLMIEEG
                     TIANRVLRDVGMDLDSVRAAVEEKTGIGSHTGDMVLGGDTQRVLELALEEAKDSGQST
                     VGTEHLLLAIARYPMCEGMKVLESLGVTPEQIRRQTGRVISQTRQDSEEDTRQAPPPD
                     FDQNQVGPSRPSSRRKEKESGKTPLVDQLATDLTALAEEGKLDPVIGRKKEIERVIQI
                     LARRTKNNPALIGEPGVGKTAIVEGLAQRIIEGDVPGLLLNKRVLQLDVGSLVAGTMY
                     RGQFEERLKRVIDELKSSGAIVFIDELHMLVGAGSAGSSVDAANILKPALSRGELQVI
                     GATTLNEYRKNIESDAALERRFQPIVVDQPSLEETIEILKGLRPNYEEHHRLHISDEA
                     LEAAAKLSSRYVTDRFLPDKAIDLVDEASSRVRMYKSESAVSSKDLMNDLRALRKDLK
                     AARAAEDNDAVQTLLAKQSALEEKLQAMQASWDRESAPTVSAEDISEVISMWTGIPLT
                     QIATEESARLLNMEEELSKRIIGQQEAIATVSKAVRRARAGLKNPRRPVGSFMFLGPT
                     GVGKTELTKALAEMLFGSQEALIQIDMSEFMERHTISRLVGAPPGYVGYEEAGQLTEA
                     LRRKPYSIVVFDEVEKAHPEALQLLLQIMEEGHLSDAKGRKVDFRNAIIIMTSNIGAD
                     LIKRQSSLGFSLEVDSALEEQSSYADMKKKLMEALKRSFRPEFVNRLDSVVVFRALNR
                     ADIREIVSLELHKVSERLQEYDLSLRSSEAALDFLSEEGFDPEMGARPVRRVIEQMVE
                     EPLSDALLARTFINGDSILVDLEESLDADGDKVRKIVLKRCETQNQPDNPELVGA"
     gene            7274..8683
                     /gene="radA"
                     /locus_tag="PKL60_04465"
     CDS             7274..8683
                     /gene="radA"
                     /locus_tag="PKL60_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNW95423.1"
                     /translation="MAKAYSRFVCQVCGKVSSQSFGRCPACDSWDSMIEEIVEPEANH
                     SARAVRGLNASSQPRRLAEIEGEREERVPVRMQEFARVLGGGIVPGSVVLIGGDPGIG
                     KSTLTLQMAMELAEQNVVLYVSGEESERQVKMRAVRLTANNASGEAALPRNLFLVTET
                     NLGVIFSHIQTVQPTLVVIDSIQTMTLPEMESSAGSISQVRECAARLRELAKSSGISI
                     FLIGHVTKEGTIAGPRVLEHIVDTVLYLEGEQFQSYRLLRSVKNRFGATSEVGVFEMQ
                     EKGMVEVSNPSEIFLAERLINAPGSAVAVTMEGTRPLLVEIQGLTTHTSFGNPRRNAN
                     GVDINRLLLITAVLTRRLGLPLGEQDVFANVVSGLRVSEPAADLATAAAIASSIKDIP
                     LRADTVLIGEVGLSGELRMVSQMPARLREAARLGFKRAVIPRRLRSNEPWPTGIEVLE
                     RRSLKDALASALLDNTDPN"
     gene            8697..9212
                     /locus_tag="PKL60_04470"
     CDS             8697..9212
                     /locus_tag="PKL60_04470"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HNW95424.1"
                     /translation="MTASFLDRLPLIGAVRRNHALEHATMQVLTEKYKGVPMAGVSNP
                     QGFLLVADLPTEIVTEAALEAEKRLKAGESQLAIHPHCGTNLVAPALAAWAAASLALW
                     AGSGSKKPRLPQFILALLTAVPVYLLARPLGPLLQKTLTTSAEQGSQRVTQVATTRLR
                     ENFLHKINTGI"
     gene            9225..10235
                     /gene="holA"
                     /locus_tag="PKL60_04475"
     CDS             9225..10235
                     /gene="holA"
                     /locus_tag="PKL60_04475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNW95425.1"
                     /translation="MELARLTVLRGDDGERVQALLAEMTASLGDPQMAEMNTTVLDSE
                     SLNLESLRADCLTMPFLAERRLVLVRKARPILTKLNSAERNKALEILAELPETAALVL
                     VIEDSQAAKRGEKYWENARAYTWLLDWLGAHAEFSQLIDCALPDDAEMPGWIAKYARK
                     AGGEFRTDAAHLLASYVGNNTLRATQEITKLLTYVNGARAVNTQDVVLLTSQEQEGNI
                     FNLVDALGERNGSKAMTQFRILSEKNEMIELSGMIYRQFRLLIQAREILDEGGGSRQV
                     EKELRVLPFVADKLTSQARRFTMAQLLDVFGRLLRIDEDMKSSGMPGEVAFELLIAEL
                     TA"
     gene            10394..11635
                     /locus_tag="PKL60_04480"
     CDS             10394..11635
                     /locus_tag="PKL60_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNW95426.1"
                     /translation="MSKYKVVPIFVVLILLFCAQGCSASPETAAPEAAIPTENPAALS
                     SSPTPIPDQSGTYAMYRGNLERSGFFTAQAPVENAGLLWQAQGAAPGASPVLAGGQLL
                     VGGPDGLSALDSQTGSLLWHSKTAGAVNSAPAYWSGMLFVGDANGILYALESQTGEQR
                     WTYQTGGSIYSSPAVEESGVYFGSMDGFFYALNPQSGQLLWKFEVAGTTDPASGISKG
                     VRNTPALSGSTLVFSSAQSGGASAELYLYAVDKSSGQKLWEYTTWDKITSPAIAEGVV
                     YVGGFGTFTGLRLSDGEPVFNFETDIVSSAPAIVDGTAIFGLDDGHVLAVSLLTGEPK
                     WSFNIGSAFSSAPSISGHMVYFGSSTGTFYAIDIPSGQEMWIYETGDRIASSGLPAEE
                     MVYFTSDNGTVYALNSRPAGE"
     gene            complement(11672..12457)
                     /locus_tag="PKL60_04485"
     CDS             complement(11672..12457)
                     /locus_tag="PKL60_04485"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNW95427.1"
                     /translation="MQPELDFITDIALEAGAMLARMQDGDLDIQLKGRADLVTRADKC
                     VEAFLIDQVRSRFPTHAIISEESGSHPGDPEHQWFIDPLDGTLNYTHGLRYYCTTIAY
                     ACAGQLQSGVIYSPREEECFTAGLGKGAFLNGKSIAVSCANRLEDALLATGFRATLID
                     TPRSNYDNFLRLSRIAQGVRRLGSAAMDLAYVACGRLEGFWDIQLSPWDVAAGVLIVR
                     EAGGIAETLYDEGSLLTNPVDILAANAQVFAPLKSILNAERER"
     gene            12668..13540
                     /locus_tag="PKL60_04490"
     CDS             12668..13540
                     /locus_tag="PKL60_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95428.1"
                     /translation="MEGALSKLPKFAVLQLSGCAGCEVSLLNASEWYDRYELVYMPLV
                     VSTHRIPDDIEVLLISGGVRNDEDAYKLQRAVKQVDRVIAVGTCAISGGVTNLGDRDE
                     VREIFLSQAKRAHLPQLLSKTHPVDAYVDVDLYLPGCPPTPELFMGALLDPQGFKAAS
                     IVCAECGRRKLNDMKPDHITGFQKGDQDPAICLVNQGFLCVGTSTRGGCRAPCTRAGH
                     PCVGCRGPSNAFIEKESGVWMENIQRVFERMTNIPSEELNEALRSPQLSMFLFQFSDY
                     AGGNRRIRSKDKVL"
     gene            13540..14853
                     /locus_tag="PKL60_04495"
     CDS             13540..14853
                     /locus_tag="PKL60_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNW95429.1"
                     /translation="MTTLTFPLSRIEGHARVVMEEEGGTLHSAQFQAVELRGFRNFVV
                     GTPAEQMPVIVPRICGVCSTAHHVASVKAIEKVFEVTPPPLACEIREILLLGQLIQNQ
                     ATSLFIFTMPDRVNVESLFDMDESEAARRAQFSIAQQALKVRKLGTDLITLAGGQFIH
                     PVKAHIGGVTGGISRQAADAMLEELRKLLPVACELFNAYWDMSLVMKERIGTWGDDEP
                     TYYISSTMSLKPKLNSGHLRVLSPDGEEKEHFTPEEFEKRIEFTQTEYSYAGQTSYQG
                     HILRANSLARINITTAFQTPLADSYLERFRASFGSPAHAILLFDLCRGIELVYAMERA
                     MELLADDLEHGETEVGYSPKDGLGYGLVEAPRGPLIHRYVVENGLIADAQFIIPTVHN
                     MKAIERALMVAGNRYIHPGTINLELEKAVGRVVRAFDPCISCATQ"
     gene            15349..>15757
                     /locus_tag="PKL60_04500"
     CDS             15349..>15757
                     /locus_tag="PKL60_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95430.1"
                     /translation="MDSKSWVKNLILTIIGGLIIAFIIGEGSLFQGRSLIDRARNLKA
                     TIDVSNSMCTIEVWNYLLVPVNVFVGEDHVGTLEASSTKKITGPFESKELSFESITPY
                     SSGLDLSGRFTSPISGDQIFQINNVIGENYYYLF"
BASE COUNT         3371 a         4592 c         4663 g         3131 t
ORIGIN      
        1 aggccagcgt ctggtcatcc gcaccggcgt ccagccgccg ctcacgccca cgcctctcgg
       61 tgcgcagccc accagcctga cccctctgcc cctgcccagc gccacgccca cgcgcacccc
      121 aacccgcacg cccatccctt cccctacccc taccgccact ccgcaatctc ctttggccgc
      181 gttttggcgg ggcaacctga gcggcgctct gctgacaggc gcgcttggca tgcttttggg
      241 cgcggggctg accttgcttc tgcgccggcg cgctccgaaa gcgccgcctg cccaggagcc
      301 agcgcgcgaa acctcagcag caaaggaacc cccgcaagga gacccggaat gagcagcaca
      361 ccccagcccc caagcccgca gcgcgcagcg ctgttggtca tcgacgtgca gcaagcgatg
      421 ttcgcccaaa gcacgcctgt ttatcaatcc gaaacattgc tgacgaatat cctctccctg
      481 accgccaaag cccggaaggc cggcgcgccc gtggtgtggg tccaacattg cgacgcgcgc
      541 gacctggtcc ccgatacgcc cggctggcag ctgcaccccg cgctggtgcc cggggcgcag
      601 gacctgaagc tccggaagca aaaaggaaac gcttttgagg gcacgaacct gaaggaactc
      661 cttgctgaac gcggcgtgag cagcgtagtc gtgtgcggga tggtcacgca cggctgcgtg
      721 aagaacacct gccttggcgc cctggcggaa ggcctgcggg taacgctcgc gcaggatgcg
      781 cacagcagtt acagcaaaga cgccgccgcg ctgattgaag aatggaacac cgcattggca
      841 caggccggcg cgcgcgtgct caacagcggc gaggtctgct tctgagcaca ggcgcacgtt
      901 ggaaatcaaa acaatcagga gaaaaacgga tgaccttacg ctccccgcag gataggatgg
      961 cggctgtctc ggaaagcatc caagagcgca ccggcaggac gctggagaaa tgggttcaac
     1021 tggcgctggc aaacggccca aacccgctgg accagaacag cgtgtgcaaa tggctgaaaa
     1081 gcgaacacgg cgtcgcgcaa aacacgcagt gggcaattgc cgaagcagcc gagctggccg
     1141 cgggttggca gcgcccgagc gaagaagaat acgcccttaa ccactactcc ggggaacccg
     1201 tctttgaaag actgcgcgcg ctgctgctcg gtctgggtcc ggacgtgcgc atggaagggc
     1261 gcggcaccta cacgcctttt gtgcgcaagc gccagttcgc ggcggtggcc gccgcgtcgc
     1321 gtgaacgggt ggacctgggc ctgcgcttca agtcggcgcc cgaaagcgcg ctgctgagcc
     1381 aggctaaagc ccccgggcaa gctacccaca agctcagcct ctgctcggtg gaagagatca
     1441 cccccgaagt ggaagccctg ctgcgcgcgg cgtatgaaca aaacggttga ttcggaattg
     1501 ttattcagat gaaaaaattc attcttaggg tcttgcttgg gtacggcctc ctcgctttcc
     1561 tgctggtagc gagcttctgc gtcatcggtt accgcgcggc aggctggcag ggcgcccgga
     1621 acgcagccgg cacgggtttg ctggttagcg caataggcct gccaatggct ggtctgctga
     1681 tcgctctgaa gttttggggc ggctatgcca accgctgggg ggcgtccaac tacaaaaaag
     1741 agttggaagg cgaacccaaa gaccgcgaac acgacccgga acgctggtag gcgcctgccc
     1801 gcctccgggg cggttttagc ccgcattcca cgaattccgt ctataattgc ggcatgcagc
     1861 ccccaaaaaa gccctctgcg ctcaagattg tcttgattgt tctgctgata tacggcatca
     1921 tcgccgtcat gatcctgctg ctcagctccc tgattggctt ctggctgaac ggcagcgagg
     1981 gagcttcttt cgcgctgggc attggcatga tgatgaatct tctcgccttc ccggccgcta
     2041 tgtacatcct ggtcgccagg ctgcgaggca tttcggacac agcccggacc ccaaaagcgg
     2101 cgggatcgaa cgagaagagt gatgaggagc gctctgcctg agcgcccaag gagaattatg
     2161 aaagcaaacc acatccccga aacccgcaca cgcaagacct ggctgattta cgtgctgctg
     2221 tcactctacg tgttcatgct caacgtcccg gggccgatca ccaactacct gaaggacgaa
     2281 ttcgcgatga gttacacgct cagcagcctg catttttcgg ctttcgcggc gggggtattg
     2341 gttaccggtt tgctcggcgc gtactttatt ccccgcttcc cgcgctggaa agtgatggca
     2401 ttcggagcgc tgggtttagg cgtcggtggg ataattctgg cctttggtca ggcgccggca
     2461 gtcacaatcc ttggcatttt tctgatgggc tgcattggta cgctgatctt gtccatttat
     2521 ccagccattc tggatgagga gatgggcgcg cgcagccccg tgggtatctc cgaagccaac
     2581 accattgcct ccctcttcgc cggcattgcc ccgctgctgg tcgggttttt ggtccaaaaa
     2641 gccgtgggct ggcgccccgc gattttattg gtcgcggcgg catccctggc attggggctt
     2701 tggctgctcc tgcggatcaa cggcagcccg gcaggcggcc tggcacagaa agttgtgccg
     2761 gaacaaaatg gaaagctgcc cggccggtat tggattttct ggctggcgct gatgcttggc
     2821 atcgcggtgg agtactgcat catctattgg ggcgcggatt ttgtagagaa agtgctcgga
     2881 ttcagcaaga acagcgccac gcaatccgtc agccttttca tggccggcat gattatcggt
     2941 cgcttctttg gctcgcgcct cctggagcgc gtgcccccga tgaagctgct caccggctcc
     3001 attttgctct ctatcgttgg tttcgcgctg ttctggagcg gtatcacccc gctgctgggc
     3061 atcgtcgggc tgacgctttt ggggctgggc ggagctaact tttaccctgt cattgtttcg
     3121 ctcgcgattg cttcggcagg ggaattgaaa gccaaagccg gcgcgcgagc gaccctggca
     3181 accggtaccg ccattctgtt cctgccgctg gttttgggca tcgtttcgga caggataggc
     3241 atccggctgg cgtacagcat cgtggcggtg ctgttcgtgc tgctctcgct cacgctgtgg
     3301 tgggcaaaca gcaggcagac cgaaccggcc gcgcaggcag aaacagcgga ttaatcgcgc
     3361 tgtgccgggc taatctcacc cgggtgaaca ggaagcttac aggaggcagg catgaaagca
     3421 ttgtttatcg gcggcacagg caacatcagc agcgcctgct caaaactggc gctggaacgc
     3481 ggcgtggaac tctatctgct caatcggggg cagtcgttta ggcccctgcc gcagggcgcg
     3541 cgcgtaatta acgccgacat ccgcgacccg aacgccgcgc aggatgctct gggcagcctg
     3601 aaattcgacg ttgtggcgga tttcatcgcc ttcaccccgc agcacatcga gactgacctg
     3661 cagctcttcc gcgggcgcgc cggccagtac ctcttcatca gttcggcctc tgcctatcaa
     3721 actccgcccg caaccttgcc tgtaaccgaa tccaccgtcc tggacaaccc ggtctggcag
     3781 tactcccggg acaaagctgc ctgcgaagag cgcctggtgc gcgcctaccg cgaggaaaaa
     3841 ttccccttta cgattgtgcg cccttcccat acctacagcg aagtttacgt gcccgtgcac
     3901 ggcaactgga cgacgattga ccgcatgctg aggggcctgc ctgtgattgt gcacggggac
     3961 ggcacctcgc tctggacgct cacccacagc tcagatttcg ccaaaggctt cgtcgggctg
     4021 ctgggcagct cgcgcgcgat tggtgaagcg taccatatca cctccgatga atggctgacc
     4081 tggaaccaaa ttcacgaaat cctggcggcg gcagcgggta cgcgggctaa tctggtgcat
     4141 gtgccgtcgg acttgattgc cgcttatgac ccaatctggg gagaaagcct gctgggggat
     4201 aaagcccaca gcttcatcct ggacaacagc aaaattaagc gcgtcgtgcc ggactttgtg
     4261 tgcagcacgc cattttcacg cggggccgaa gaaattctcg cctggcaccg cgccgacccc
     4321 gcgcgccaaa aggtggacga aaactttaac gcaatttgcg aacgcatcct cacagcatac
     4381 gccaaagcct ggcctgaaaa gtaaccgcga aaacaaaaaa gtgggattaa cgcatgcgct
     4441 cgccggcgcg gagcaccgcg ttgcgtccga acaaagctcc gtgctggcag ctggcgtatt
     4501 aacgtttcat cataatctct ggacagagga aaaagcgggg ggataatttg gtaaaatgac
     4561 agtatcagag gattccaaat caaacctttg actgaccgcg cttgaccgca ggtcttaccc
     4621 gcaagatttc ttcctgccga aatccacact acggggaggg gcggcagggg ttcttttcgg
     4681 agccacaaaa taactggagg tatgccttgg ctggcattaa tcgttttaca caaaaagcca
     4741 agcgggttct cgcccatgct cagactgaag ctgagcggat gcataagcaa accataggga
     4801 cggagcacct tttacttgga ttgatgattg aagaaggcac gattgcgaat cgtgttttgc
     4861 gcgacgtggg catggatctg gattccgtcc gcgccgcagt cgaagaaaag accggcattg
     4921 gcagccatac cggcgacatg gtgcttggcg gcgatacgca gcgcgtgctg gaactggcgc
     4981 tggaagaagc aaaagacagc gggcaatcca cggtcggcac agaacatctg ctgctggcta
     5041 tcgcgcgcta cccgatgtgc gagggtatga aagtgctgga aagcctcggc gtcacgcccg
     5101 aacaaatccg ccggcagact ggtcgggtga tttctcaaac ccggcaggac agcgaggaag
     5161 acacccgtca ggcgcccccg cccgactttg accagaacca ggttggcccc agccgacctt
     5221 cgtcgcgccg caaggaaaag gaaagcggga aaaccccatt ggtggaccag ctggcaacag
     5281 acctgaccgc cctggcggaa gaaggcaagc tggacccggt tatcgggcgc aaaaaggaaa
     5341 tcgagcgcgt catccagatt ctggcccgcc gcaccaaaaa caacccggcg cttatcggtg
     5401 aacccggcgt gggcaaaact gccatcgtcg aagggttggc gcagcgcatc atcgaaggcg
     5461 acgtgcccgg gctgctgctg aacaaacgcg tactgcagct ggacgtcggc tcgttggtgg
     5521 cgggcacgat gtaccgcggt cagttcgaag agcgcctgaa gcgcgtgatt gacgaactga
     5581 aaagctccgg cgccattgtg ttcatcgacg aactgcacat gctggtgggg gccggttccg
     5641 ccggctcgtc cgtagacgca gccaacatcc tcaaacccgc cctttcccgg ggggaacttc
     5701 aggtaattgg cgccaccacc ctgaacgaat accgtaagaa tatcgagagc gacgccgcgc
     5761 tcgagcgccg ctttcagccc atcgtggtcg accagccctc gctggaagaa acaattgaga
     5821 ttctgaaggg tctgcgcccg aattacgaag agcaccaccg cctgcacatc tcggacgaag
     5881 ccctggaagc cgccgccaag ctctcctccc gttatgtgac agaccgcttt ttgcctgata
     5941 aggccatcga cctggtggac gaagcctcct cgcgcgtgcg catgtataag agcgaatccg
     6001 cggtgagcag caaagacctg atgaacgatt tgcgcgcgct ccgcaaagac ctgaaagccg
     6061 cccgcgcggc ggaagataac gacgcggtgc aaacactgct ggccaaacaa agcgctctgg
     6121 aagagaaact acaggccatg caggcctcct gggaccgtga aagcgccccg accgtgagcg
     6181 cggaagatat cagcgaggtc atctccatgt ggaccggcat tccgctgact caaatcgcga
     6241 ccgaagaatc cgcccgcctg ctcaacatgg aagaggaact ctccaaacgc atcatcgggc
     6301 agcaggaagc tattgcaacg gtctccaagg cagtacgccg ggcgcgcgcc gggctgaaga
     6361 acccgcgcag accggttggt tcgttcatgt tccttggccc gacgggcgtc gggaaaacag
     6421 aattgaccaa agcgctggcg gaaatgctgt ttggcagcca ggaagccctg attcaaatcg
     6481 atatgtccga attcatggaa aggcacacca tcagcagact ggtcggcgcg ccccccggat
     6541 atgtcggtta cgaagaagcc gggcagctga ccgaagcgct gcgccgcaag ccctacagta
     6601 tcgtggtgtt cgacgaagtg gaaaaagccc accccgaagc gctgcagctg ctgctgcaga
     6661 ttatggaaga ggggcacctc tccgacgcga aagggcgcaa ggtggacttc cgcaacgcca
     6721 tcatcatcat gacctccaac attggcgcgg acctgataaa acgccagtcc tcgcttggtt
     6781 tctcattgga agtggacagc gcgctggagg aacaatccag ctacgctgac atgaagaaga
     6841 agctgatgga agcgctcaag cgcagcttcc ggcctgaatt cgtgaaccgt ctggactccg
     6901 tagtcgtctt ccgggcgctc aaccgcgcgg atatccgcga gattgtcagc ctggagctgc
     6961 acaaggtctc cgagcgcttg caggaatacg acctgtccct gcgcagcagc gaagccgcgc
     7021 tggattttct ctcggaagaa ggctttgacc ccgaaatggg cgcccgtccc gtgcggcgcg
     7081 taatcgaaca gatggtcgaa gaaccgctct ccgacgctct gcttgcccgc acctttatca
     7141 acggcgacag catcctggtg gacctggaag aaagcctgga cgcggacggc gacaaggtgc
     7201 gtaaaatcgt gctcaaacgc tgcgaaacgc aaaaccagcc ggacaatccg gaattggtgg
     7261 gggcgtagca gcgatggcaa aagcttactc ccgttttgtc tgtcaggtgt gcggcaaagt
     7321 cagttcccag tcttttgggc gctgcccggc ctgcgacagt tgggacagca tgattgagga
     7381 aattgtagaa ccggaagcca atcattcagc ccgcgcggtg cgcgggctga atgcgtcttc
     7441 ccagccgcgc cgcctggctg aaatcgaagg cgaaagggaa gaacgcgtgc cggtgcgcat
     7501 gcaggaattc gccagggtgt tgggcggggg gattgtgccc ggttcggtcg tgctgatagg
     7561 cggcgacccg ggcatcggaa agtccacgct caccctgcag atggcgatgg agttggcgga
     7621 acaaaacgtc gtgctctacg tctccggtga ggaatcggaa cgccaggtga agatgcgcgc
     7681 ggtgcgtctg accgcgaaca atgcctccgg cgaggcagcc ctgccgcgga atttgttcct
     7741 ggtgacggaa acaaaccttg gggtgatatt ttcgcacatc cagacagtcc aacccacgct
     7801 ggtggtgatt gattccattc agacgatgac cctgccggaa atggaatcct ccgccgggtc
     7861 catttcccag gtgcgcgaat gcgccgcccg gctgcgcgag ctggccaaat ccagcggtat
     7921 ctctattttc ctgatcgggc atgttaccaa agaaggcacc attgccggtc cgcgcgtctt
     7981 ggagcacatc gtggatacag tgctctacct ggaaggcgaa cagtttcagt cctaccgcct
     8041 gctgcgctcg gtcaagaatc gctttggcgc cacgtcggaa gtgggcgtgt tcgagatgca
     8101 ggaaaagggc atggtggaag tcagcaatcc gtccgagatt ttcctggcgg agcggctgat
     8161 taacgcgccc ggctcggcgg ttgccgttac catggaaggc acgcgccccc tgctggtgga
     8221 aatccagggg ctcaccacgc ataccagctt cggcaacccg cgccgcaacg cgaacggggt
     8281 ggacatcaac cgcctgctgc tcatcaccgc ggtgctcacc cgccgcctgg gcttgcccct
     8341 gggcgaacag gacgtatttg ccaacgtggt cagcggcttg cgcgtttccg agcccgctgc
     8401 cgacctggcg accgcagccg cgattgcctc gtccatcaaa gatatcccct tgcgcgcgga
     8461 taccgtcctg ataggcgagg taggactctc gggcgagctg cgcatggtca gccagatgcc
     8521 cgcccgcctg cgcgaagccg ctcgcctggg cttcaaacgc gcggtaattc cccgccgcct
     8581 gcgctccaac gagccctggc caaccggcat cgaagtgctg gagcggcgtt ccctgaagga
     8641 cgcgcttgct tccgctttat tggataacac agacccgaat tgaaaggcga acaccaatga
     8701 ccgcttcatt tttggataga ctgccgctta ttggcgccgt aaggcgcaat cacgccctgg
     8761 aacacgccac catgcaggtt ttgacggaaa aatataaagg cgtccccatg gcgggggttt
     8821 ccaatccgca gggattcctg ctggtggctg acctgccgac tgaaattgtc accgaagccg
     8881 ccctggaagc cgagaagcgc ttgaaagccg gcgaaagcca gctcgcaatc catccgcact
     8941 gcggcaccaa cctggtcgcg ccagcgctcg ctgcctgggc ggctgcctcg ctggcattat
     9001 gggctggcag cgggtcgaag aagccacgcc ttccccagtt catcctggcg ctgctcaccg
     9061 cggtgccggt gtacctcctg gcccgcccgc tgggtccgct gctgcaaaag acgctcacca
     9121 ccagcgccga gcagggcagc cagcgcgtca cccaggttgc taccacgcgc ttgcgcgaaa
     9181 actttctgca caaaatcaac acggggattt aacaggcggg gcagatggaa ttagcgaggc
     9241 tcacagtact gcgcggagat gacggcgagc gcgttcaggc gcttctggcg gaaatgactg
     9301 ccagcctggg agatccgcag atggcggaga tgaacaccac cgtattggac agtgaaagtt
     9361 tgaacctgga gagcctgcgc gccgactgcc tgaccatgcc tttcctggcg gagcgccggc
     9421 tggtgcttgt gcgcaaagcc cgcccgattc tgacgaagct gaacagcgct gaacgcaaca
     9481 aagcgcttga aatcctggcg gagttgccgg agactgccgc gttggtcctg gtcatcgaag
     9541 actcacaagc cgcaaaacga ggggaaaaat attgggagaa tgcccgcgcg tatacctggc
     9601 tgctcgattg gctcggcgcg cacgcagaat tcagtcagct catcgactgc gcgctgcccg
     9661 acgacgcgga aatgccgggt tggatagcaa aatacgcgcg caaagccggc ggggaattcc
     9721 gcaccgacgc ggcgcacttg ctggcaagct atgtcggaaa taacactctg cgcgcgaccc
     9781 aggaaataac caaactgctg acctacgtaa acggcgcgcg cgccgtcaac acccaggacg
     9841 ttgtcttgct gacctcgcag gagcaggaag gcaatatttt taatctggta gacgccttgg
     9901 gggagcgcaa cggctcgaaa gcgatgaccc aatttcgcat cctgagcgaa aagaacgaga
     9961 tgattgagct ctcaggcatg atttaccgcc aattccgtct gctcatccag gcgcgggaga
    10021 tattggacga gggcggcggc agccggcagg tggaaaaaga gctgcgcgtt ctgccttttg
    10081 tggcggataa actgaccagc caggcgcggc gctttacgat ggcgcaactt ctggatgtgt
    10141 ttggaaggct tctgcgaata gacgaagaca tgaaaagcag cggcatgccc ggcgaggtcg
    10201 cgttcgagct gctcatcgcg gaacttaccg cctgagcgaa aaaaccgcag ctcagcgcgc
    10261 cgtcttccat gctgattccc aacgatgcgt tcgcattacg cgtgaattca cttttatacc
    10321 cgcgataatt catcgtatac tactatcaat ctagtgattt caaaattgaa tacgcatcag
    10381 tggaggtgtt cagatgtcga aatacaaagt cgtgcccatt ttcgttgttc tcatcctgct
    10441 tttttgcgcc caggggtgca gcgcttcgcc tgaaactgcc gcacctgaag ccgccatccc
    10501 aacggaaaac ccggcagcgc tcagctcaag cccaacaccg ataccagatc aatccggaac
    10561 ctatgcaatg taccggggaa acctggagcg cagcggtttc tttacagccc aggcgcccgt
    10621 ggaaaacgcc ggcttgctgt ggcaggcaca aggcgcagct ccgggcgcat cgccggtgct
    10681 ggcaggcggt cagctgctcg ttggcggccc ggatggatta tccgcgctgg acagccaaac
    10741 cggcagcctc ctatggcact ctaagactgc gggcgcggta aactcagcgc cggcttattg
    10801 gtctggcatg ctgtttgtcg gcgatgctaa cggcatcctc tacgctctgg aaagccagac
    10861 cggcgagcag cgctggacct accagaccgg cgggtcgatt tattcctccc ccgcggttga
    10921 ggagagcggt gtgtacttcg gcagtatgga cggatttttc tacgcgctga acccgcaaag
    10981 cgggcagctg ctgtggaagt ttgaggtggc tggaaccaca gaccccgctt cgggaatcag
    11041 caaaggggtg cgcaacacgc ccgcgctgag cggcagcaca ttggtgtttt ccagcgcgca
    11101 aagcggcggg gccagcgcgg agctgtacct ctacgccgtg gataagagct ccgggcagaa
    11161 gctgtgggag tacaccacct gggacaagat tacaagccct gcgattgcgg agggtgtggt
    11221 ttatgttggc gggttcggca ccttcacagg gctgcggctg agcgacggtg aacctgtgtt
    11281 taactttgag acggatattg tgtcgagcgc gccagcgatt gttgacggga cggcgatttt
    11341 tggcctcgat gatgggcatg tgctggccgt ctccttactc accggagagc caaagtggtc
    11401 attcaatatt ggcagcgcgt tctcctcggc gccttcgata tccgggcata tggtttactt
    11461 tggtagcagc acgggtactt tctatgccat cgatatcccc tccgggcagg agatgtggat
    11521 ttatgagacc ggagaccgaa tcgcgtcctc cgggctgccc gctgaagaaa tggtgtactt
    11581 caccagcgac aacgggactg tatacgcttt gaacagccgc ccggcaggtg aataaggctt
    11641 atcggggcat aaagacggcg gaataccgcg cttagcgctc gcgttccgcg ttcaatatgg
    11701 attttagcgg cgcgaagacc tgcgcgttgg cagccagaat atccaccgga ttggtaagca
    11761 ggctgccctc atcatagagc gtttcggcaa tgccgccggc ttcgcgcacg atcaggacgc
    11821 cagccgccac gtcccacggg ctcagctgga tatcccaaaa gccctccagc cggccgcagg
    11881 ctacataagc aaggtccatc gccgcggacc cgagccgccg cacgccctgc gcgatccgcg
    11941 agaggcgcag aaagttgtcg tagttcgagc gcggggtgtc aatcagcgtc gcccggaatc
    12001 cagtagccag cagagcatcc tccagtctgt tggcgcagga aaccgcgatg cttttgccat
    12061 tcaggaaggc ccctttgccc agcccggcgg taaagcactc ctcctcgcga ggggaataga
    12121 taacacccga ctgcagctgc ccggcgcacg cgtacgcgat ggtcgtgcag taataacgca
    12181 gcccgtgcgt gtaattcaac gtgccgtcca gcgggtcgat aaaccattga tgttccggat
    12241 cgcccgggtg gctgccgctt tcctcggaga taatcgcgtg ggtggggaag cggctgcgca
    12301 cctggtcaat caggaacgcc tccacgcact tatcagcgcg ggttaccagg tcagcgcgcc
    12361 ccttcaactg gatatccagg tcgccgtcct gcatgcgtgc cagcatcgct ccagcttcca
    12421 gggcaatatc ggtaatgaaa tctagttcgg gctgcatcag ttccaccttg tgcgcaatat
    12481 cgtgatggta ttgtagcatc aaacccgcag gctccgggca cattccgagc ttgcgctctg
    12541 cggctttccc ccgcatttgc tgcccctaaa tcccctcgat gagggtaaaa tctattgtgg
    12601 ataagttgtc gataaatccc tggtttttgt ggataaactc ctcatttctg tggaaaagga
    12661 gatgcttatg gaaggcgccc tgtcaaaact ccccaagttc gcggtgctgc agctttcggg
    12721 ctgcgcgggt tgcgaggttt cgctcctgaa tgcttcggaa tggtacgacc gttatgaatt
    12781 ggtgtatatg ccgctggtgg tctccacgca ccgcatcccg gacgacatcg aagtactgct
    12841 gatttccggt ggggtgcgca atgacgagga cgcctacaaa ctccagcgcg cggtgaaaca
    12901 ggtggaccgc gtcattgccg tgggcacttg cgccatctca ggcggagtaa ccaacctggg
    12961 cgaccgcgac gaggtgcgtg aaattttcct ctcgcaggcc aagcgcgcgc atttgccgca
    13021 gctgctcagc aaaacgcacc cggtggatgc ttatgtggat gttgacctct atctgccggg
    13081 ctgcccgccc acgccggagc tctttatggg tgcgctgctg gacccgcagg gctttaaggc
    13141 cgcctctatc gtctgcgcgg agtgcgggcg gcgaaaattg aacgatatga aaccggacca
    13201 tatcaccggc ttccagaaag gggaccagga ccccgccatc tgtctggtga accagggctt
    13261 tctatgcgtg ggcacctcca cgcgcggcgg ctgccgggcg ccctgcacgc gcgccgggca
    13321 cccctgcgtg ggatgccgcg ggccgtccaa cgctttcatc gagaaagaat ccggcgtctg
    13381 gatggagaac atccagcgcg tgtttgagcg catgaccaac attccatctg aggaattgaa
    13441 cgaagcgctg cgctcgcccc agctttccat gttcctgttc cagttctccg actacgcggg
    13501 cggcaaccgc cgcatccgca gcaaggataa ggtgctctga tgaccacgct cacatttccc
    13561 ctcagccgga ttgaagggca cgcccgcgtg gtgatggagg aagaaggcgg cacgctgcac
    13621 agcgcgcaat tccaggcagt ggaactgcgc ggcttccgca actttgtggt cggcacgcca
    13681 gccgaacaaa tgccggtgat tgtcccgcgc atttgcggcg tgtgttccac cgcgcaccac
    13741 gtcgcctccg tgaaagctat cgagaaagtt ttcgaggtca ctcctccgcc gctggcttgc
    13801 gagattcgcg agatcctgct gcttggtcag ctgatccaga accaggccac ctcgcttttc
    13861 atcttcacta tgccggaccg cgtgaatgtg gaaagcttgt tcgacatgga cgaaagcgaa
    13921 gcagcccgcc gggcgcagtt ttccatcgcg cagcaggcgc tgaaggtgcg caagctcggc
    13981 accgacctga ttacgctggc gggcgggcag ttcatccacc cggtcaaggc gcatatcggc
    14041 ggcgttaccg gaggcatcag ccggcaggcc gcggacgcaa tgctggaaga gctgcggaaa
    14101 ctgctgcctg tggcttgcga gctgtttaac gcctactggg atatgtcctt ggtgatgaaa
    14161 gaacgcatcg gcacctgggg ggatgacgag ccgacgtatt acatatcctc caccatgtcc
    14221 ttaaaaccaa aattgaacag cggacatctg cgcgtgctca gcccggacgg cgaggaaaag
    14281 gaacacttta cccccgagga atttgagaaa cgcatcgaat tcactcagac cgagtattct
    14341 tacgccgggc agaccagtta ccaggggcac atcctgcgcg ccaacagcct ggcgcgcatc
    14401 aacatcacca cagctttcca gacaccgctg gcagacagct acctggaacg cttcagggcc
    14461 agttttggca gccccgccca cgcgattttg ctctttgacc tgtgccgcgg gattgagctg
    14521 gtgtatgcca tggagcgcgc gatggagctg ctggcggacg acttggaaca cggcgagacg
    14581 gaagtgggtt acagccccaa agacgggctt ggctacggcc tggtggaggc cccgcgcggt
    14641 ccgctgatac accgctacgt ggtggaaaac ggcttgattg cggacgcgca gttcatcatc
    14701 cccacagtgc ataacatgaa ggcgattgaa cgcgcgctga tggtggcggg caaccgttat
    14761 attcatccgg gcacgattaa cctggagctg gagaaggcgg tcgggcgggt ggtgcgggcg
    14821 tttgacccat gcatctcctg cgcgacgcag taaggggggt attaatttgc ttgtcggtgg
    14881 agagtggaaa ctctgaagaa aagtgtagtg gaacaaccat cgataactta catttccttc
    14941 tcagtaaact tggttgttgg tatgaaaatc cattactact tggtaagtaa tgtttgtaat
    15001 aaccaaactg tttttaacgc tagctgtttt tcagttgaga cgaataatgt caacactcct
    15061 gatttttata ttgtagatca aacatttaag gcatattcat taatgtgtag acattgagga
    15121 tacattcaca tttcattgct tagttgtgtg aaaaacatcc gaccagtcaa tatggtttct
    15181 taactgatct ccgtttatgt aaaggtccca gacgcgttgt gattgttgag ttcatagttt
    15241 ataagaagaa tactattttc cactttttgg aaagtgggct acatttttga gtgtataatt
    15301 tttatacttt atttctgagt tcctcaacat tctgagaaag gaacagggat ggacagtaaa
    15361 tcttgggtca agaatttaat cttaacaata ataggtgggt taattattgc tttcatcatt
    15421 ggtgaaggtt cgctttttca aggaaggtca ttgattgatc gtgcaagaaa tctgaaagcg
    15481 acaatcgatg tctccaattc aatgtgcaca atcgaagtgt ggaactacct tcttgtcccc
    15541 gtcaatgttt ttgttggtga agatcatgta ggaactcttg aggcaagttc cacaaaaaaa
    15601 attacaggtc cgtttgaatc aaaagaatta tcatttgaga gtattacgcc ttactcgtct
    15661 ggtctagatt tgtctggtcg tttcacttcc cccatttcag gtgaccaaat atttcagatc
    15721 aataatgtca tcggtgaaaa ttactattat ttatttg
//
LOCUS       DAOQAD010000037        15659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_4030_length_15659_cov_6.580364, whole genome shotgun sequence.
ACCESSION   DAOQAD010000037 DAOQAD010000000
VERSION     DAOQAD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15659
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4030_length_15659_cov_6.580364"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PKL60_04505"
     CDS             <1..738
                     /locus_tag="PKL60_04505"
                     /inference="COORDINATES: protein motif:HMM:NF022635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HNW95431.1"
                     /translation="LEMSFCEVVPAQRSAVEYLELVMSHLPGVFYLGGHSKGGNLAAF
                     AGANIPTDLQPGILRVYNNDGPGFQRSVIESPGYRRIANRIVNFLPQSSVVGMLLDQS
                     GLYQVVASTETGFMQHNAFSWEVIGRSFVYREGLSASSIKFNAALREWLDSVPFEERE
                     EFITVLFTILEATGARTLKEFTQDKLASAKAMIASFKQLEPETQAQMKALLETFFSEI
                     QKTLRSSITESISSLITRTFTRKRLPS"
     gene            1040..1300
                     /locus_tag="PKL60_04510"
     CDS             1040..1300
                     /locus_tag="PKL60_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNW95432.1"
                     /translation="MNDNQVSQYSKEEKIAGLIDQISAYIEQYHRGSVELVSIEGDLV
                     KVRLGGACEGCPLLPATLQGWVAGTIRQFFPDVTVEAVENPC"
     gene            complement(1320..2207)
                     /locus_tag="PKL60_04515"
     CDS             complement(1320..2207)
                     /locus_tag="PKL60_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNW95433.1"
                     /translation="MNPRWWDGGAVVKPGETFEARSLDTPLDEIVRKQGGRRSETYTD
                     RKRGRYIQARPSPHKAYDLAFDATFRAAAPFQKERAELRTQEQIAFAVRPNDYQKKVR
                     VKKTANLILFLVDGSWSMAVAERMAATKGAILSLLNDAYQRRDRVGLVVFQKDRATLV
                     LAPTNSVLLAQRALTDIPVGGKTPLAAGLVMAYEVITRERILHPDVQPLLIVLTDGAG
                     NVPLGKKPVKEEIRELAGLFSRKNIHSVVINMEKKAFDQGLAQELADQMGAACYTLEE
                     IRGETLLNTVQKEMQTAQA"
     gene            complement(2270..3385)
                     /locus_tag="PKL60_04520"
     CDS             complement(2270..3385)
                     /locus_tag="PKL60_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW95434.1"
                     /translation="MNQRVIYPFSAIVGQDRMRRALILNAVYPRIGGVLIRGERGTAK
                     STAARALAALLPEEPVVEGCRFGCSPYDPSHWCTECLERDAASDQPLPYVMRPTPFVN
                     LPVSATEDRVVGTLDIERAIQKGERHFEPGVLAAANRGLLYIDEVNLLDDHVVDILLD
                     SAAMGMNIVEREGISFSHPARFILVGTMNPEEGDLRPQLLDRFALSVEIRGILDARSR
                     VEIMQRNVAFEQDAERFRQEWLPQEQALSAQIQKARQLVDAVAYTPRDLLVIADLTSS
                     MEVDGHRSDLVILKAARAQAAFDGRARINQNDIALAAELALPHRVKSGPFRKSDLGVE
                     ALEERIEQLKGQVASEEEGSPSEGDEGKKKLTPWMDP"
     gene            complement(3511..4686)
                     /locus_tag="PKL60_04525"
     CDS             complement(3511..4686)
                     /locus_tag="PKL60_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HNW95435.1"
                     /translation="MNANRQDIVIAGIGQTDVGELWDVSLRDLGVRAIQAARQDAGGL
                     APQALYIGNMLAASASHQANLGALLCEWAGLKGAEGTTVEAADASGGAALRMAYIAVA
                     SGMVDVAAAVGVEKATDVLGSDIESLSTRNLDADFEANEGQTPVGQAALLMQRYLHEN
                     QFPREASAAFPVIAHANAVNNPHAMYPKAIKPESYLKSGLVNDPFNLYDIAPLGDGAA
                     AVVLTRAELLPKGLAHAPVRLAGSSLVVDRLALHDRQDPLFFEAAAVSVQRACLKAGI
                     SLADVDFFEYSDITTLHAILSLEAAGFARRGQGWKLASDGSLLLTGSLPVATLGGHKA
                     RGNPIGAAGVYQAVEACLQLRGEAGGSQVAHARFGMIQSLAGTGSTAVTHILERRDA"
     gene            complement(4694..5782)
                     /locus_tag="PKL60_04530"
     CDS             complement(4694..5782)
                     /locus_tag="PKL60_04530"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HNW95436.1"
                     /translation="MTEHTHQQDKSALLLKPDRQVGIIGYGAYVPRYRLPAKEIARIW
                     SAGDEGLPVIEKAVAGLDEDVITMSVEAARNALKRAGIDPAELRAVWVGSESHPYAVK
                     PSGTIVAEAIGASVNIQAGDWEFACKAGSEAMVAAMAMVGSGMGRYALAIGMDTAQGK
                     PGDALEYTAASGGAAYIFGPADESLASVEAAYSYVSDTPDFWRRAHAVYPEHGQRFTG
                     EPAYFQHLLTASRTIMEASGLKPADFTYAVFHQPNTKFPQRAGKELGFSPEQIKTGLL
                     SPVIGNTYAGASLIGLSAVLDAARPGDLILMTSYGSGAGSDSFILRVREPILERRGKA
                     LLTQDYIARRTEIDYATYVRFRGEIVVR"
     gene            complement(5775..7319)
                     /locus_tag="PKL60_04535"
     CDS             complement(5775..7319)
                     /locus_tag="PKL60_04535"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HNW95437.1"
                     /translation="MTASKIVSEFWFDIIQSFIFTKFLKYIHFNWRFTRTPKLCCRIQ
                     RQRSRISFKPADYCKPDSFLETLDDKQYWSLAKCRVYEWSRITLEPKKFYQMSLTERR
                     EWLQSQAQLSAEDIHAWSPQGGLSAERADHMIENAVGVFALPLGIAQNFLVNGRKVLV
                     PMAVEEPSILAGVSFMARLAQAGGGFFAWAGAPEMIAQIQMLDIQDMDAAQAAILAHK
                     TELLTCLKGLHPTIEGLGGGARDLQVRRIENSAIGPFLVVHLIYDVRDVMGANAVNSA
                     AEALAGPLEQLTGGRAHVRILSNLADRRLARAEVRIPSAALAFDRYTGEQVRDGILEA
                     WAFAAVDPYRAATHNKGIMNGVDAVVIATGNDWRGVEAGAHSYAARAGTYTSLSTWSK
                     GTQGELCGALELPLALGITGGSTQSHPTARANLRLINARSAGELAGIIAAVGLAQNLA
                     ALRALATEGIQRGHMRLHARQVALSAGASPEEAGRLAAQLAREGNIRIERAREILDDW
                     RKNKHD"
     gene            7279..8241
                     /locus_tag="PKL60_04540"
     CDS             7279..8241
                     /locus_tag="PKL60_04540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW95438.1"
                     /translation="MSNQNSETIFDAVIIGGGPAGSTAGIYTARANLSTLILDKGLTT
                     GALGTTSKIANFPGIPGEIAGAELLRTIRQQAESFGVKYVNDKAIGADLSADPKLVFG
                     NFGVYSARAVVIATGSMGRSNLIAGEAELLGRGVSYCAVCDAAFFRQAVVAVAGKSDE
                     AVEEALYLSRFASKVYLFCPAPELSAPAELLQEMRANPKIELLTGATLREIYGGERVE
                     GVRYAQRGSESEQDLPLNGVFIYLQGGVPITDFLQGQLETNPGGCLRVDREFQTSKPG
                     VFAVGDVLCQHIKQAVIAASDGATAGIAVEKYLRGRAKMGVDWK"
     gene            8291..9037
                     /locus_tag="PKL60_04545"
     CDS             8291..9037
                     /locus_tag="PKL60_04545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW95439.1"
                     /translation="MPHVIPEDLNKAAIRGEPSRVWRAGQQRRLDMLLKAAGELVKGR
                     VLVDGCGVGTYLSRLAPICGQTLGLDIEFPRVQDSRAFSRLVVCGAGESLPFETGSID
                     LVFSHEVLEHVQDDQAAVAEMVRVLKPGGRVVLFCPNRGYPFETHGVYWRGRYHFGNI
                     PLVNYLPRRWRDKLAPHVEIYTRRDLARLFRGQPVRIIEKRTIFGAYDNIIERRPRLG
                     RALRGLLQQLEKTPLQRLGLSHFWIVEKTV"
     gene            complement(9042..10400)
                     /locus_tag="PKL60_04550"
     CDS             complement(9042..10400)
                     /locus_tag="PKL60_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNW95440.1"
                     /translation="MTNINSSNFLYHLFPLGACGAPKTNDFSSAPVSRLCQIHGWLDH
                     IQSLGADTLFLGPVFESGTHGYDTANYYEVDRRLGTRADLAELGRDLRARGMRLVLDG
                     VFNHTGRDFWAFRDLQTRGAASAFRDWYANVRFDQRSPFGDPFSYDAWNGHFSLPKLN
                     LENPAVREHLFGAVRMWVEEFGIDGIRLDAADALNFAFLRALREFSEQLKPGLWLMGE
                     VIHGDYRQWANEQTLHSTTNYELYKGLYSAHNEHNYFELAYSLNRQFGAQGIYQGLEL
                     NSFVDNHDVTRIASQLREPAALTPLHVVLFSAPGVPSVYYGSEFGLAGVKSRDDWNLR
                     PEFKPHALAKAAPQPELFAWIQKLALIRKNSPALREGAYVQRLVAADQFAFSRHTAGE
                     DVLVLVNNADATVDISVPLAGFEGKGFRDLLTPGEAFWTDAHANLTVRLYPKSGRILR
                     RD"
     gene            complement(10466..11032)
                     /locus_tag="PKL60_04555"
     CDS             complement(10466..11032)
                     /locus_tag="PKL60_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNW95441.1"
                     /translation="MNNRTTILETALSFFAAFGYEATGTLEICDAAGVSKPTMYHYFK
                     SKRGLMEAILSGNFQPFLERLKEASRYQHDLTLNLERVMRAYFSFAQENPVFYRLQFS
                     MQYAPLKSESYELVLPWVKRQREPIQAMFAQAEADHGNMRGRSSGYTTTLLGMINAYA
                     SDALARERELDDELIYQARHQFMHGIFS"
     gene            11175..11705
                     /locus_tag="PKL60_04560"
     CDS             11175..11705
                     /locus_tag="PKL60_04560"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HNW95442.1"
                     /translation="MRTKQVVVVPWQPEWPARFEEIAAALLPALEGLICAVEHVGSTS
                     VPGLAAKPIIDIDIIIPDLSAFPRVRGALAALGYRHEGDLGIPGREAFKYDGKPELMA
                     HHLYVCTRDAAELRRHLALRDYLCAHPQDRERYAQVKLAAAARYPTDIDAYIEEKSAI
                     IQEIYSRCGLEPEPEA"
     gene            11959..12714
                     /locus_tag="PKL60_04565"
     CDS             11959..12714
                     /locus_tag="PKL60_04565"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW95443.1"
                     /translation="MDNISKFTGKGAVYQQYRPDYAAEALRYLKEKLGIGPGAAVADV
                     GSGTGIFTRQLLLLGARVYAVEPNSDMRAEAERQLADFPGFYSVAGRDSNTTLPDQSV
                     NLVSAAQAFHWFEPALFREECRRILKPGGKVLLVWNQRDQSSPVNQVSKAIFAEYCPD
                     FKGFSGGMSQRDARIEGFFPHGYALLQFTYPIYYTREGYIGRCLSASYAILPEDPRYE
                     PFVAALGQVFDRFQQDGKLLLPNTTVFHIGSVA"
     gene            12743..13324
                     /locus_tag="PKL60_04570"
     CDS             12743..13324
                     /locus_tag="PKL60_04570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW95444.1"
                     /translation="MELEIIPLSPESGPEFCAFFDHLDYSYKPDWRGCYCQFYHSTCD
                     EAGWQARSPGQNRADALARIARGEMRGYLAYADGECVGWCNANALAALPRFAGSPELA
                     QFDDKTGVVICFVIRPDFRGRGAARQLIRAAVEGFRREGFACVLGLPFEWKEHPELQY
                     HGTRAMFADAGFSRVNTDAQRPLMVLDLSEEKS"
     gene            complement(13352..13633)
                     /locus_tag="PKL60_04575"
     CDS             complement(13352..13633)
                     /locus_tag="PKL60_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95445.1"
                     /translation="MSKALTPTARIAKAREFIRQAREIPVPDSAGWDYFSYTAQVKDT
                     LKRAFELVKLIQYSPSTPPEVKAEARALIDSLPDVEKEILKGGSADGQA"
     gene            13856..14461
                     /locus_tag="PKL60_04580"
     CDS             13856..14461
                     /locus_tag="PKL60_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95446.1"
                     /translation="MKKLLILSLTVVLLSLAACGGSGVAGGTSSYQSTLTALESENAS
                     KETALQAEIASLKAQVTDLQNQQQSLKDERDAAVEKADKYYSMFIVENADDEGDYIFC
                     EQAFETSFGYADKVSMRMELIKYLANLRGYNPEAMSSEHQTIWSNADDIIVRVNGGGY
                     MFPFLVRFGDGGLGNLDAVYDLTNGCYVDFPYLEAQFLSVR"
     gene            15248..>15659
                     /locus_tag="PKL60_04585"
     CDS             15248..>15659
                     /locus_tag="PKL60_04585"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558517.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNW95447.1"
                     /translation="MSDQNNKKRFDLDENEIPRFWYNINADSPVAPAPVFDPATKEPI
                     SLEALLKLFPMPLIEQEASLERFIPIPDEVREAYKLYRPTPLIRATRLEKALQTPAHI
                     YYKYEGASPAGSHKLNSAIPQAYFNKISGTRAMTT"
BASE COUNT         3158 a         4787 c         4609 g         3105 t
ORIGIN      
        1 ctggaaatga gtttctgcga ggtggtgccg gcacagcgca gcgcggtaga gtatcttgaa
       61 ctggttatgt cgcacctgcc gggcgtcttt tatctgggcg ggcattccaa aggcggaaac
      121 ctggctgcct tcgccggcgc caacatcccc accgacctgc agcccggcat cctgcgagtg
      181 tacaacaacg acggccctgg ttttcaacgt tccgtaatcg aaagccccgg ctaccgccgc
      241 atcgcaaaca gaattgttaa cttcctgccg cagtcatccg tggttggcat gctgctggac
      301 caaagcgggc tctaccaggt ggtcgccagc accgaaaccg gtttcatgca gcacaacgct
      361 ttctcgtggg aagttatcgg tcggagcttt gtgtatcggg aagggctttc cgccagcagc
      421 ataaagttca acgccgccct gcgcgagtgg ttggacagcg tgccttttga ggaacgcgaa
      481 gaattcatca ccgtgctatt caccattctg gaagcgacag gcgcccgaac tctaaaggaa
      541 tttacccagg ataagctcgc aagcgccaaa gcgatgattg cctctttcaa gcagctggag
      601 ccagaaacac aggcacagat gaaggccctg ctggaaactt tcttttccga aattcaaaaa
      661 acactgcgca gttccatcac ggaaagcatc agcagcctta tcacgcgcac cttcacgcgc
      721 aagcgcctgc cttcctgaaa gttgcggcgg gcgcacgttt ggattcgtca gcgcgaagga
      781 ggccggggat taaagcaatt tcccttgctg cccgcccaaa ctgcagggtt taccagtatc
      841 gctctcattc ccgtaatcag gttatctttt tgtagacagg caggcgactg ctggcaaagc
      901 atacagcccg acgctccgcg cggcagcgat gaacggaaaa aaccgctaaa agctcccacc
      961 cgcccgcgga gcagaccgtc ctgcatctgt gataagattg tcttgagtac tccgccagca
     1021 ataaaggatt aaacaagaaa tgaacgacaa ccaggtctcg caatacagca aagaggaaaa
     1081 aatcgccggc ctgattgacc agatcagcgc ttatatcgaa cagtaccacc gcggttcggt
     1141 ggaattggtc tccatcgagg gtgatttggt aaaagtacgc ctgggaggcg cctgcgaagg
     1201 ctgcccgctg ctgccggcca ccctgcaggg ttgggtcgcg ggcactatcc gccagttctt
     1261 cccggatgtg acagtcgaag cggtcgaaaa tccctgctga gcaatcctct ccccccgggc
     1321 taagcctggg cagtctgcat ttccttttgg acggtattca acagcgtttc tccgcggatt
     1381 tcttccaggg tgtagcacgc cgcgcccatc tggtccgcca gttcctgcgc cagcccctgg
     1441 tcaaaggctt ttttctccat attaatcacc acgctgtgga tattcttcct ggaaaacagc
     1501 cctgcaagct cccggatttc ctccttgacc ggctttttcc ccaaaggcac gttgcccgcg
     1561 ccgtcggtga gcacaatcag taacggctgc acatccgggt gcaggatgcg ctcacgggta
     1621 atcacctcgt acgccatcac cagccctgcc gccagcggcg tcttgccgcc caccgggata
     1681 tcggtcagcg cccgctgcgc cagcagaaca gagttggtcg gcgcgagcac cagcgtggcg
     1741 cggtctttct ggaaaaccac cagccctacc cggtcccgcc gctggtaggc atcgttcaac
     1801 aaggatagta tcgcgccttt ggtagcagcc atgcgctccg ccaccgccat agaccacgag
     1861 ccgtccacca gaaagaggat cagattggcg gtcttcttca cgcgtacttt tttctggtaa
     1921 tcattggggc gcaccgcgaa tgcgatttgt tcctgcgtgc ggagttcggc gcgctctttc
     1981 tggaaaggcg cggcggcccg gaaggtggca tcgaaagcca ggtcgtaagc tttatggggg
     2041 gaagggcgcg cctggatata gcgcccgcgt ttgcggtcgg tataggtttc agaacgccga
     2101 cccccttgtt tgcgcacaat ttcgtccagg ggagtatcca atgaacgggc ttcaaaagtc
     2161 tcgcccggtt tgacgacagc ccccccatcc caccagcgcg ggttcaattc ggtgaatatc
     2221 tgctgctgcc cctcgccgac cggaatcagc gggtcctccg ggtccaggct tacgggtcca
     2281 tccagggcgt cagttttttt ttaccttcat cgccctcgga gggtgagcct tcttcttcgc
     2341 tggcaacctg gcccttcaac tgctcaatcc gttcctccag cgcttccacg cccaggtcgg
     2401 atttacggaa ggggccggat ttgacgcggt gcggcagcgc cagttcggca gccagggcga
     2461 tgtcgttctg gttgatgcgc gcgcgcccgt caaaagctgc ctgggcgcgg gcagccttca
     2521 ggatgaccag gtcggagcgg tgcccgtcga cctccatcga tgacgtcagg tcggcgatga
     2581 ccaacaggtc ccggggggta taagccaccg cgtccaccag ttggcgcgct ttctggatct
     2641 gggcggagag cgcctgttcc tgcggcagcc attcctgacg gaaacgttcc gcgtcctgct
     2701 caaaagcgac gttgcgctgc atgatttcca cgcgcgagcg cgcgtccagt atgccgcgga
     2761 tttccaccga cagggcgaag cggtccagca gctgcgggcg caaatcgcct tcctccgggt
     2821 tcattgtgcc caccaggatg aagcgcgcgg gatgactgaa ggaaatgcct tcgcgttcca
     2881 cgatgttcat gcccatcgcg gcggaatcga gcaggatatc aaccacatgg tcgtccagca
     2941 ggtttacttc gtcaatgtag agcagcccac ggttggcagc cgccaaaacg ccgggctcaa
     3001 aatgccgttc gcccttctga atggcccgtt cgatatccag ggtgccgacc acgcggtcct
     3061 cagtggcgga aactggcagg ttcacaaaag gcgtcgggcg catcacgtag ggcagcggct
     3121 ggtctgaagc ggcgtcgcgt tcaagacatt ccgtgcacca atggctgggg tcgtacgggc
     3181 tgcagccaaa gcggcagcct tccaccacgg gctcttccgg cagcagcgcc gccaatgcgc
     3241 gcgctgcagt ggatttggcc gttccgcgtt cgccccgaat caacacgccg ccaatgcgcg
     3301 gatacactgc gttcaggatg agcgccctgc gcatacggtc ctgcccgaca atcgcggaaa
     3361 acggataaat gacgcgttga ttcataggga attgttcctt taccttttta agtttaccac
     3421 gcgtgaatat taaagacagc ctgccctaac gcaggctgta catcccgatg aggctgccgg
     3481 agaaatccgc cccctcccgc cgcgctgttt ttaagcgtcc cggcgttcca gaatgtgcgt
     3541 gacagcggtg gaaccggtgc cagccagtga ctgtatcatc ccaaaacggg catgcgccac
     3601 ctggcttccg ccggcttccc cgcgcagctg caggcaggct tccacggcct ggtagacgcc
     3661 ggccgcgcca atcgggttgc cgcgcgcctt atgcccgccc agcgtggcca ccggcaggga
     3721 gccggtcaaa agcaggctgc cgtcgctggc cagtttccag ccctgcccgc gccgggcaaa
     3781 gccggccgcc tccagagaga ggatagcgtg cagggtggtg atatcggaat actcaaaaaa
     3841 gtccacatcc gccaggctga tgccggcttt caggcaggcg cgctggaccg agaccgccgc
     3901 cgcctcaaaa aacagcgggt cctgccggtc gtgcagcgcc agccggtcca ccaccaggct
     3961 ggaacctgcc agacgcaccg gcgcgtgcgc caggcctttc ggcagcagct ccgcgcgggt
     4021 aagcaccacc gcggccgcgc cgtctcccag cggagcgata tcgtacaggt tgaaggggtc
     4081 gttcaccagc ccggatttga ggtagctttc gggtttaatg gctttgggat acatcgcgtg
     4141 cggattattg accgcgttgg catgcgcgat gaccggaaaa gcagctgaag cctcacgcgg
     4201 gaattgattt tcgtgcaggt agcgctgcat cagcagcgcg gcctgaccta caggcgtttg
     4261 cccctcatta gcctcaaaat ccgcgtccag gttgcgcgtg ctcagggatt cgatgtcgct
     4321 gccgagcacg tccgtggctt tttccacgcc caccgctgcc gcgacgtcca ccatgcctga
     4381 ggcgactgcg atataggcca tgcgcagcgc cgccccgccg gaggcgtcag ccgcctccac
     4441 cgtggtgcct tcggcgcctt tcaaaccagc ccactcacac aggagcgcgc cgagattggc
     4501 ctgatgcgag gcactggcag ccagcatgtt gccaatgtac agcgcctgcg gagccagtcc
     4561 gccggcatcc tggcgggcgg cttgtattgc gcgcacgccc aaatcccgca gggagacgtc
     4621 ccataattcg cccacatcgg tctgcccgat gcccgcgatg acaatatcct gtctgttcgc
     4681 gttcatcaca gacctaccgg acgacaatct cgccgcggaa gcgcacatag gtggcgtaat
     4741 cgatttccgt gcggcgggct atgtaatcct gcgtgagaag cgccttgccg cgccgttcca
     4801 ggatgggttc gcgcacgcgc aggataaagg aatccgaacc cgctcccgag ccgtaggagg
     4861 tcatcaatat caggtccccc ggtcgggcgg catccagcac cgcgctcaag ccaatcaggg
     4921 acgcaccggc gtaagtgttg ccaatcaccg gtgaaagcag cccggtcttg atttgttccg
     4981 gactgaagcc gagctctttg ccagcgcgct gcggaaactt ggtattcggc tggtgaaaaa
     5041 ccgcgtaagt aaaatctgcc ggctttaatc cggatgcttc cataatcgtc cgggaagcgg
     5101 ttagcaggtg ttgaaagtaa gcgggttcgc cggtaaagcg ctgcccgtgc tccggataga
     5161 ccgcgtgcgc ccggcgccag aagtccggcg tatcggagac gtacgagtaa gcggcttcca
     5221 cggatgccag cgactcatcc gccggaccga aaatataggc cgccccgcct gaggcggcgg
     5281 tgtactccaa cgcgtcgccg ggcttgcctt gggcggtgtc catccctatc gccagcgcgt
     5341 accggcccat ccccgagccg accatggcca tcgccgccac catcgcttcg gagccggctt
     5401 tacaggcaaa ttcccagtcc cccgcctgga tgttgactga agcgccgatg gcttccgcca
     5461 cgatggtgcc gctgggtttc accgcgtacg ggtgcgattc ggagcccacc cagacggcgc
     5521 gcagttccgc cgggtcaatg ccggcgcgct tgagcgcgtt gcgggcggct tccaccgaca
     5581 tggtgataac gtcctcgtct aaaccggcga cggccttttc gatgaccggc agcccctcat
     5641 cgcccgcgct ccaaatgcgc gcgatttctt tggcgggcaa gcggtagcgc ggtacgtacg
     5701 cgccgtaacc aatgatgcca acctgccggt ccggtttcag cagcagcgct gacttatcct
     5761 gttgatgcgt gtgttcagtc atgcttgttc tttctccagt cgtccaggat ctccctggca
     5821 cgttcaattc gaatattccc ttcgcgcgca agctgggcag ccagacggcc agcctcttcg
     5881 gggctggcgc ccgctgagag cgccacctgc cgggcatgca ggcgcatatg cccgcgctgg
     5941 atgccctcag tcgccagggc tcgcagcgcc gccaggtttt gcgccaaacc caccgccgcg
     6001 ataatcccgg ccagttcgcc cgcgctgcgc gcgttaatta gcctcaggtt cgcgcgggct
     6061 gtcgggtggc tttgggtgga gccgccggtg atgcccagcg ccaacggcag ttccagcgcg
     6121 ccgcaaagct cgccttgcgt gcctttgctc caggtggaga ggctggtata cgtccccgcg
     6181 cgggcagcat agctgtgcgc gccagcttcc accccgcgcc aatcgttgcc cgtcgcgatg
     6241 accaccgcgt ccacgccgtt catgatgcct ttattgtgcg tggctgcccg gtaagggtca
     6301 acagcggcaa aagcccaggc ttccaggatg ccatcgcgca cctgttcgcc tgtgtagcgg
     6361 tcaaacgcca gcgctgcgga ggggatgcga acttccgcgc gcgccaaccg gcggtccgcc
     6421 aggttggaga ggatgcgcac atgcgcccgc ccgccggtaa gctgttccag cggtcccgcc
     6481 agcgcttccg cggcggagtt taccgcgttg gcgcccatga cgtcgcgcac atcataaatc
     6541 agatgcacaa ccaggaaagg accaatcgcg gagttctcaa tccgccgcac ctgcaggtcc
     6601 cgcgcgccgc cgcccaggcc ttcgatggtt ggatgcaggc ctttcaagca agtgagcagt
     6661 tcggttttgt gcgccagaat cgcggcttgc gcggcgtcca tgtcctggat atccagcatc
     6721 tggatttgtg caatcatctc gggcgcgcca gcccaggcga agaaaccccc gcccgcctgc
     6781 gccaacctgg ccatgaacga cacgcccgcc aggatggagg gctcttccac cgccatcggc
     6841 accaaaacct tgcgcccgtt aacgagaaag ttctgggcaa tgcccagggg cagcgcgaac
     6901 accccgaccg cgttttcgat catgtggtcg gcccgttcag cgctcaggcc cccctgcggg
     6961 gaccaggcat ggatgtcctc cgcgctcaac tgcgcctggc tttgcagcca ctcccggcgc
     7021 tccgtcaggc tcatctggta gaactttttg ggttccaaag ttattctgct ccactcatag
     7081 acccgacact ttgccaggct ccagtattgt ttatcatcca gggtctctaa aaaactatca
     7141 ggcttgcaat aatctgccgg tttgaatgaa attctactgc gttggcgctg gattcgacag
     7201 caaagcttag gcgtccgggt gaatcgccag ttgaagtgga tatacttcag gaacttagtg
     7261 aagataaagg actgaattat gtcgaaccaa aattctgaga cgatttttga tgcagtcatc
     7321 atcggcggcg gacctgccgg ttccacagcc gggatttaca cggctcgcgc caacctgagc
     7381 acgctcatcc tggataaggg actgaccacc ggagcgctgg gcaccaccag caaaattgcc
     7441 aacttcccgg ggatccccgg cgaaatcgcc ggcgccgagc tgctgcgcac gatccgccag
     7501 caggcggaaa gcttcggcgt gaagtacgtg aacgacaaag ccatcggagc cgacctctcg
     7561 gctgacccaa aattggtgtt cgggaacttc ggggtgtact cagcgagagc cgttgtcatt
     7621 gccaccggtt ccatgggcag aagcaacctg attgcagggg aagcggaatt gctgggcagg
     7681 ggcgtttcgt actgtgcggt gtgcgacgcg gcctttttca ggcaagccgt ggtggcagtg
     7741 gcgggcaaga gcgacgaagc cgtggaggaa gcgctgtatc tgagccgctt tgcgagcaag
     7801 gtctacctct tttgcccggc acccgaactc tccgccccgg cggaattgct gcaggaaatg
     7861 cgtgccaacc ccaaaattga gctgctcacc ggcgccaccc tgcgcgagat ttacggcggg
     7921 gaacgcgtgg agggcgtgcg ctacgcccag cgcggcagcg aaagcgaaca agacctgccg
     7981 ctgaacgggg ttttcatcta tctgcaaggc ggagtgccaa tcactgactt cctgcagggg
     8041 cagctggaaa ccaacccggg cggctgcctg agggtggacc gcgaatttca gacttcaaaa
     8101 cccggcgtgt ttgccgtggg ggatgtgctt tgtcagcata tcaaacaggc agtcatcgca
     8161 gcttcagacg gcgccacagc gggcatcgcg gtagaaaaat acctgcgcgg gcgcgccaag
     8221 atgggcgtcg actggaagta atcccagagc agcctgccta accaggccca ctcttggtac
     8281 aatagctctc atgccgcacg ttatccctga ggatttgaac aaagccgcca tccggggcga
     8341 gccttcgcgc gtctggcgcg ccgggcagca gcgccgcctg gatatgcttt tgaaggccgc
     8401 cggcgagctc gtaaaaggac gcgtgttggt ggacggctgc ggcgtgggca cctacctgag
     8461 ccgcctggct cccatctgcg ggcagacgct tgggctggat attgaattcc cgcgcgtgca
     8521 agacagccgc gctttttccc gcctggtggt gtgcggagcg ggcgaatccc tgccctttga
     8581 gaccggcagc atcgacctgg tattcagcca cgaagtcctg gaacacgttc aggatgacca
     8641 ggctgccgtc gcggagatgg tgcgcgtgct caaaccgggc gggcgggtgg tgctgttttg
     8701 ccccaaccgc ggttaccctt tcgaaaccca cggggtttac tggcgcgggc gctatcattt
     8761 cggcaatatc ccgctggtaa actacctgcc gcgccgctgg cgggataaac tcgccccaca
     8821 cgtggaaatc tacacccgcc gggacctggc gcggctgttc cgcggccagc cagtccggat
     8881 tattgagaag cgcaccattt tcggggcata tgacaacatc attgagcgcc gtcccaggct
     8941 ggggcgcgcg ctgcggggcc tgctgcagca gctggaaaaa acaccgcttc agcgcctggg
     9001 tctttcccac ttctggattg tcgaaaagac agtctgaccc cctaatcccg ccgtaaaatc
     9061 cggccgcttt tggggtacag ccgcaccgtc aggtttgcgt gcgcgtcagt ccaaaacgct
     9121 tcccctggtg taagcaaatc acggaaacct ttgccctcaa agccggcgag cggaacgctg
     9181 atatccacgg ttgcgtcagc attattcacc agcaccaaaa catcctcccc cgcggtgtgg
     9241 cgggaaaacg cgaactggtc cgccgcgacc agcctttgaa cgtaggcgcc ctcgcgcagc
     9301 gccgggctat ttttacggat tagggcaagc ttttgaatcc aggcgaagag ctcaggctgc
     9361 ggcgcggctt tagccagcgc gtggggctta aattccggac gcaaattcca atcatcgcgg
     9421 cttttcacac ccgccaagcc gaattcgctg ccgtaataga ccgagggcac cccgggcgcg
     9481 ctgaaaagca ccacatgcaa gggggtcagc gcggccggct cccgaagctg gctggcaatg
     9541 cgcgttacgt catgattatc cacaaagctg ttcagctcca ggccctggta aatcccctgg
     9601 gcgccaaact ggcggttcag gctgtacgcc agctcgaagt agttgtgttc attatgcgcg
     9661 gagtacaagc ctttgtagag ctcgtaattg gtggtgctgt gcagggtttg ttcgttcgcc
     9721 cactggcggt aatccccatg aatgacctcg cccatcagcc acagcccggg cttgagctgc
     9781 tcgctaaact cccgtaaggc ccgcagaaac gcaaaattca gcgcgtccgc cgcgtccagg
     9841 cggatgccgt cgatgccgaa ttcttccacc cacatccgca ctgccccaaa caggtgttcg
     9901 cgcacagccg ggttctccag gttgagcttg gggagggaga agtgtccgtt ccaggcatcg
     9961 taagaaaagg ggtccccaaa ggggctgcgc tggtcaaagc gcacgttcgc gtaccaatcc
    10021 cggaaagcgg aagccgcgcc gcgcgtttgc aggtcgcgga aagcccaaaa atcccggccg
    10081 gtatggttga acacgccgtc caacaccagg cgcatgccgc gtgcgcgcag gtccctgcca
    10141 agttccgcca ggtcggcgcg cgtacccagc cggcggtcca cttcataata gttggcggtg
    10201 tcgtagccgt gggtgccgga ttcgaacacc ggcccgagga agagcgtatc cgcgcccaga
    10261 gactggatat ggtccagcca gccgtggatt tggcagagcc tcgataccgg cgcgctggaa
    10321 aaatcattgg tctttggcgc gccgcaggcg cccaggggga agaggtgata aaggaaattg
    10381 ctgctgttaa tgttggtcat ggttgctccg tgattatacc gtacggtata gtgatacagg
    10441 taaaaaaata agccctgagg cgccctcacg agaagatacc gtgcatgaac tgatggcggg
    10501 cctggtaaat cagctcgtcg tccagttcgc gttcgcgtgc cagcgcgtcg ctggcgtagg
    10561 cgttaatcat gcccagcagg gtggtcgtgt agccggatga tctgccgcgc atattgccgt
    10621 ggtcggcttc cgcctgcgcg aacatggcct ggattggctc gcgctggcgc ttgacccagg
    10681 gcaagaccag ctcgtagctc tcgctcttta agggcgcgta ctgcatggaa aattgcaggc
    10741 ggtagaagac cgggttttcc tgtgcgaaag agaaatacgc gcgcataacc ctttccaggt
    10801 tgagcgtcag gtcgtgctgg tagcggctgg cctccttcaa gcgctccaga aagggctgaa
    10861 agttcccgga gagaatggct tccatcaagc ctcgcttgct cttgaagtag tggtacatgg
    10921 tcggcttgct cacgccagcc gcgtcgcaga tttccaatgt gccggtagcc tcgtagccaa
    10981 acgcggcaaa aaacgacagg gcggtctcga ggattgtcgt gcggttgttc atcgccgcag
    11041 agtataccgc tcggtatagt atctgtcaag ggcaaacccg cgcgtgataa gattgttgtg
    11101 cggaagtatg cccccgcgcg gcggcgtctt cgggtcaagt ccttttgctg agaaaacagg
    11161 agcttacgga aagcatgcgc acaaaacagg ttgtggtcgt cccctggcag ccggaatggc
    11221 cggcgcgctt cgaggaaatc gcggcagcgc ttctgcctgc gctcgagggg ctcatctgcg
    11281 ccgtcgaaca cgtcggcagc acttccgtgc ccgggctggc ggccaagccc atcatcgata
    11341 tcgacatcat tatcccggac ctttcagcgt tcccgcgggt tcggggcgcg ctggccgctt
    11401 taggctaccg tcacgaaggc gacctgggca tccccggccg ggaagctttc aaatatgacg
    11461 gcaagccgga actgatggcg catcatctct acgtttgcac gcgtgatgcg gccgaactgc
    11521 gccggcacct ggccttgcgg gactacctgt gcgcgcaccc gcaagaccgc gagcgctacg
    11581 cgcaggtcaa gcttgctgcc gccgcgcggt accccaccga tattgacgcc tacatcgaag
    11641 agaagtccgc catcatccag gagatttact ccagatgcgg gctcgagccg gagcctgaag
    11701 cctgaagagg ccgctgtgaa cacacgcgct gttccacccg cccgccatcc attacgaatt
    11761 cggctccccg ctttcgcgcc cgtcaagagc gggacgagcg cgctcgattt ccgggtcgga
    11821 ttgctgcaag tcgaagaatc tgcggttgtt cgcgcgcgcg ctcagcctgg atatatataa
    11881 tgggagcaag cgcgctggaa caaaaccagc gctggctgcg ggaatgcctg attgaacctg
    11941 gagaggagcg ttttagcctt ggataacatc tcaaaattca ccggaaaagg cgctgtctac
    12001 cagcagtacc gcccggatta cgccgcagaa gccctgcgct acctgaagga aaagctgggc
    12061 atcggcccag gcgcggcagt cgcggacgtg ggttcgggca ccggcatctt cacgcgccag
    12121 ctgctgctcc tgggtgcgcg agtgtacgcg gtggagccaa actccgatat gcgcgccgaa
    12181 gcagagcgtc aactggctga tttcccgggc ttttactccg tcgccggacg cgattccaac
    12241 acgactttgc ccgaccagag cgtaaacttg gtcagcgccg cccaggcttt ccattggttc
    12301 gagccggcat tgttccggga ggaatgccgg cgcatcctga agccgggcgg gaaggtgctg
    12361 ctggtttgga accaacgcga ccaatccagc ccggtcaacc aggtcagcaa ggctatcttc
    12421 gcggagtact gcccggactt taagggcttc agcggcggga tgagccagcg cgacgcgcgc
    12481 atcgagggct tcttcccaca tggatatgcg ctgctgcagt tcacttaccc catctactac
    12541 acccgggaag ggtacatcgg ccgctgcctg tccgcttcgt atgccatcct gccggaggac
    12601 ccgcgttatg aaccgttcgt ggcggcgctg ggtcaggttt ttgaccgctt ccaacaggac
    12661 ggaaagctgc tgctacccaa tacaaccgtt tttcacatcg gcagcgtcgc ttagccgggc
    12721 acgaatcgga aaggatggat taatggagct tgaaattatc ccgctcagcc ctgaatcagg
    12781 accagaattt tgcgcttttt tcgaccatct ggattacagc tataaaccgg actggcgcgg
    12841 ctgctactgc cagttctacc acagcacctg cgatgaagct ggctggcagg cgcgcagccc
    12901 cgggcaaaac cgcgcggacg cgctggcgcg catcgcacgg ggagaaatgc gcggctatct
    12961 ggcttatgcg gatggcgagt gcgtcggttg gtgcaacgcc aacgctttgg ccgcgcttcc
    13021 gcgctttgca gggagccccg agctggccca gtttgacgac aagaccggcg tggtgatctg
    13081 ctttgtcatc cgcccggact ttcgcggcag gggcgcggcg cgccagctca tccgcgcggc
    13141 ggtggaaggc ttccgtcggg aaggtttcgc gtgcgttttg gggctgccct tcgaatggaa
    13201 agagcacccc gaactgcaat accacggcac ccgggcgatg ttcgcggacg ccggcttttc
    13261 gcgggtgaac acggacgcgc agcgcccttt gatggtttta gatttgtcag aggaaaaaag
    13321 ctaagccggc tgcaggcgct gctggcaatc ctcaagcttg cccgtccgcg ctgccgcctt
    13381 tcaggatttc tttttccaca tccggtagag agtcgattaa cgcgcgggct tcggctttca
    13441 cttcgggcgg cgtgctgggg gagtattgga tcagcttgac cagctcaaag gctcttttga
    13501 gcgtatcctt gacctgcgcg gtgtagctga agtaatccca gccagccgag tccggaacgg
    13561 ggatctcgcg ggcctggcgg ataaactcac gggctttggc gatgcgggcg gtgggggtta
    13621 gggcttttga cataggcaga taatcttccc gggttgagcg aaacgcaaaa agaaaaattt
    13681 ttaatttgtc tcttcaaaac atgtaggaat cagaaaaaac cattaatttt aaacttttta
    13741 cccgtattca tattataatt gcaaaagcag cggatattca ttatccaacc tcggatttgg
    13801 taaaaaaatc cgaaagaacc cttcaacgtt atgattttga ttcaggagga aagttgtgaa
    13861 aaagctatta atcctcagtc tgaccgttgt tttgctgagt ctggcagcct gcggtggttc
    13921 cggagttgcc ggcggcacgt cgtcgtatca gtccacgcta accgccttgg aaagtgaaaa
    13981 tgcaagcaaa gaaaccgctc ttcaggctga gattgccagt ttgaaggctc aagtgactga
    14041 cctgcaaaat cagcagcaat cgctcaagga tgaacgcgat gcagccgtgg agaaagcgga
    14101 caagtattat tccatgttca tcgtggaaaa cgcggacgat gagggcgatt acattttttg
    14161 cgagcaggca tttgagacgt cctttgggta tgccgataaa gtttccatgc gcatggaatt
    14221 gattaagtat ctcgccaatc tgcggggcta caatcctgaa gcaatgtcct ctgaacacca
    14281 gaccatttgg agcaacgcgg atgacatcat cgtccgggtg aacggcgggg gttacatgtt
    14341 cccgttcctg gtgcgcttcg gggacggcgg cctggggaat ctggacgcag tctatgacct
    14401 gacaaacggc tgctatgtgg acttcccata cctggaggcg cagttcctgt cagtgagata
    14461 aggcagctca tttggaagca aacccgcctg gcagtcactt ccatgctcga tgcagcaatc
    14521 ggagcacagc gtgccagcgg gttttctttt ttccggaatc ctgcctcaga tttgttcgtg
    14581 caggatctat tacggaaccg ctgcagtctg gaggtttatt tggaaagcgc gactgtcagc
    14641 tccagctttc aagcctgaag ctcttgacac ccgccctttt ccgcctataa ttatgctcaa
    14701 tcaattatta aaagactttg acgaggacta gtagccgcag aaccggtggc acagagagca
    14761 ggaccagctg aaaacctgcc cacacgaccg cgacgaatgg acctccgagt tgccgggcga
    14821 acgcgctgcc agtagcccgt gccggagctg ccgccgttat ccaggcaagg atatcgcgtt
    14881 tttcgccgta tccgaaaaga gtgaggctgg ctttccgttg tgttcatcac cggaaatttt
    14941 tcttaaaaag acagcctaag ctgggtggca ccgcggattt ttcacacatc cgtccctgag
    15001 agggatgggt gtgtttttga ttaaccggaa aaggagcgcg cgtgaaccag catcatgatc
    15061 catttaccag acaagccgca atattctgcc acgccgccca cgaggtccgc ccccgacgtt
    15121 cagcttcttt ccatgccgcc ggaacgccca cccgcaaacc agactcaagc cgtggattaa
    15181 acgcgaattc gcgcaagcct ttcgcgccgc acaaactgca tcattccaat acaaaggaga
    15241 actgatcatg tctgaccaaa acaacaagaa acgctttgac ctggacgaaa atgagattcc
    15301 acgcttttgg tacaacatca acgccgacag cccggtggcg cccgcgccgg tctttgaccc
    15361 cgccaccaag gagcctatca gcctggaagc gctgctcaag ctcttcccga tgcccctgat
    15421 tgagcaggaa gccagcctgg aacgcttcat ccccatcccg gacgaggtgc gcgaagccta
    15481 caagctctac cgccccaccc cgctcatccg cgccacccgg ctggaaaaag ctttgcagac
    15541 ccccgcgcat atttattaca aatacgaagg cgcctcgccc gcaggcagcc acaagctcaa
    15601 ctccgccatc ccgcaggcgt acttcaacaa aatcagcggc acgagagcca tgaccaccg
//
LOCUS       DAOQAD010000038        15536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_4079_length_15536_cov_6.629223, whole genome shotgun sequence.
ACCESSION   DAOQAD010000038 DAOQAD010000000
VERSION     DAOQAD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15536
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4079_length_15536_cov_6.629223"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..3921
                     /gene="nifJ"
                     /locus_tag="PKL60_04590"
     CDS             322..3921
                     /gene="nifJ"
                     /locus_tag="PKL60_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNW95448.1"
                     /translation="MKHQMVMTDGNEATAHVAYRLNEVIAIYPITPSSAMGEYADQWM
                     AERVPNLWGTIPTVAQMQSEGGAAGAVHGALQAGALSTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHISARSLAAQALSIFGDHQDVMACRQTGWAMLSSGSVQEAQDCALISTAAT
                     LRARVPFLHFFDGFRTSHEVNKIERITDEELRAMVDDDLIIEHRKRALSPDAPVVRGT
                     AQNPDVYFQGRETVNTYYDACVGIVSQEMEKFGKLTGRPYHIMEYCGAPDAERVMIIM
                     GSGAQTASETAELLAKQGEKVGVIYVRLYRPFSVKHLMAALPATVKKIAVMDRTKESG
                     ALGEPLYQDVVNAVSVAMDEGTAPFQHKPVIIGGRYGLSSKDFTPAMAKGVFDELKKD
                     HPKRQFTVGIDDDVTHLSISYDPSFEALGNDVIQAMFFGLGSDGTVGANKNSIKIIGD
                     ETENHAQGYFDYDSKKSGTVTVSHLRFGPKPIKAPYLISEGEATFVGCHQVSFVDRFD
                     MLKYARKGSVFLLNSIYSPEEVWDTLPKQMQQDLISKEIEFYVIDAYSVAEKTGMGQR
                     INTIMQTCFFAISKILPRDEAIAAIKHSIEKTYGKRGEAVVNKNFAAVDQAIANMHRV
                     KVPAQVTSTIERHSPVPANAPDYVKDVIGTIIDRKGDSIKVSQMPVDGTFPTATTKWE
                     KRNIALEIPVWEPDVCIQCARCSFVCPHAAIRVKIYPTELLANAPEGYKSTASKAREW
                     KDGYSWTVQVAPEDCTGCGLCVQNCPGKDKTDPNRKAINLAPQPPIREREMKNWDFFL
                     SLPMLDRTTIPTHLIKNSQLLEPLFEFSGACAGCGETPYIKLATQLFGDRMVIANATG
                     CSSIYGGNLPTTPYSKNRDGRGPAWSNSLFEDNAEFGMGMRLTIDKLAVYARELLGKH
                     APEVGAELANAILSASQKSEAEIQEQRKRVRELKAALEGKTEQELKNLLSVADYLVRK
                     SVWIIGGDGWAYDIGYGGLDHVLASGKDVNVLVLDTEVYSNTGGQSSKATPRAAVAKF
                     AADGRDKPKKDLGLISMSYGNIYVAHVAMGSSDNQTLRAFVEADSYEGPSLIIAYSHC
                     INHGIDMSKGLVQQKLAVNSGAWPLYRYDPRLADSGKNPLQLDSKAPSIKVSEYAYNE
                     TRYRMLLQSDEERAEMLMKAAQADAERRWSLYEQMAAMDYSFGKPAEGEAPQA"
     gene            4059..5063
                     /locus_tag="PKL60_04595"
     CDS             4059..5063
                     /locus_tag="PKL60_04595"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNW95449.1"
                     /translation="MAELKTSYLGLPLKNPLVAGASPFTLQLDVVKQLEDSGVSALVM
                     HSLFEEEVTHESLELDYFLNKGTESQPEAQTYLVEPRRIESIADRYIAQLQKIKESVE
                     IPVIASLNGVTPGGWVRLAKDMQAAGADALELNIYTLVTDPGLNSDQVEKNYISLIQE
                     VCKQVNIPVAVKLSPFLTTLPHFLARAYKAGAEGFVLFNRYMQPNFDIESLEVERKAR
                     LSTNADLALPARWIAIASSQLKADFALSGGVHSPEDMVKAIMAGAKVAYVVSALLANG
                     VKHAARLLSGLSVWMDRNGYESVEKMLGTLRLDKIENPEAYERANYLRTLKSFDERVI
                     "
     gene            5101..7053
                     /gene="dnaG"
                     /locus_tag="PKL60_04600"
     CDS             5101..7053
                     /gene="dnaG"
                     /locus_tag="PKL60_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNW95450.1"
                     /translation="MGVVDEIKAQLDLVDYISQTVKLRRTGKNYIGFCPFHSNTRTPA
                     FVVFPESQTWRCFGQCNEGGDLFGFVMKKEGWDFNETLRFLAEKAGVALSSQPRIAEA
                     DAQARDVLIKVLEEAAQFYRSQLVESEAGGAALAYLRHRGLSDTTIKIWGLGYAPAGW
                     DELTRQLTRKGYTREHLLRAGLLTEREDGGTHDKFRHRLMFPIRDTYGKMAGFGGRIL
                     NPDDVPKYLNSPRTELFDKGRLLYGLDLARQEIRKAEQAVIVEGYMDVIGLHQAGFGN
                     AVSPMGTALTEDQFKLLKKFTRSIILALDPDAAGEKATLRGLETARQTMDQDDQPTFD
                     SRGLLHFEGRLKADIRVTTLPAGQDPDEIALADPEAWRKIASDAKPVVVHVMETLAAA
                     QDLNDAKVKREIAARVLPLIEDVGDAVERDAYRQQLARLLRVDERSLVLANPVQRRAR
                     RSSEEHGRSELESSLNAITEKARSNRMLEEQALRTLVQEPYSLNEINRALGLMSVPQL
                     GDEDFVESDFRQAFRVVLNSIRQDQLSPQDYLAENLPDLFSSEEPAAMIEKSLPPSAQ
                     KRLADQVRTVLRIKRSHLETRIQEILFLQSELEEKRYSAEEAEVLLTELIVQRRTLDE
                     AMKSKMAGGDGMEERKPRHKPLSMGK"
     gene            7057..8517
                     /locus_tag="PKL60_04605"
     CDS             7057..8517
                     /locus_tag="PKL60_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNW95451.1"
                     /translation="MDDDLQFEAEDDEKFEAGSEPHETLSPSEEYTPEEESGTQSDEP
                     IQLYLREINRSRLLEAQEEFQLAICVQAEKIQSLFIKPDNSLKLPGLYASIANLSEKI
                     TAASAALKKEPLDLSQVLAEASALRRNFQDALPSYTHNFLQDERWKQDAEWQNLARLL
                     CSFLTAVYLLPVPFLNSVAELTARHGRFPSWEELSEIQPDPDEMALSTRQVRDIAKLA
                     RRDLVEFNLRLVVSVAKRYTGRGINIEDLIQEGNMGLLRAVEKFDPARGFRFSTYATW
                     WIRQSISRYILEYARTIRIPVHMVESISKLIKAQHMLVQELGREPNFAEIAAASGLLS
                     SEDEQEIRRNAFSREALPADLLRRWDEAALKVEQILKVGEEPVSLESPVGDEDNSTLG
                     DYIEDADAPEPMEAVILESLRESVRQSLSDLTEKERAILELRFGLVDGVYHSLEEISE
                     KFGLTRERIRQIESTALRKLRDPRRMNSLRDFFQSS"
     gene            complement(8809..9453)
                     /locus_tag="PKL60_04610"
     CDS             complement(8809..9453)
                     /locus_tag="PKL60_04610"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNW95452.1"
                     /translation="MKTIPRILLLSLAVVLAFVGIGAITTPVSAQTDSAETLSEAERS
                     TLLHMAAEEKLAHDVYLALYEKWELAAFQRIARSETRHFDRMFQLLGDYGINSNLDQL
                     GAGEYGDPQFDQLYDDLVAKGSLSEEEALKVGGYIEERDILDLEKAIDESTHADVQRA
                     YRRLLQGSSMHLRAFVRGWEKMTNAVYEPQVMTAEQLETYQAFRGPMRGCRGRR"
     gene            9582..9926
                     /locus_tag="PKL60_04615"
     CDS             9582..9926
                     /locus_tag="PKL60_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNW95453.1"
                     /translation="MTEKPAKHGEDQDGLPLYQRVYALVKQIPAGKVATYGQIARMLG
                     GCSARMVGYAMSALRDGRDPEVPWQRVINAQGRISLHGIGSAEQRFLLEDEGVVFDEG
                     DRVSFDRYGWEG"
     gene            9901..10107
                     /locus_tag="PKL60_04620"
     CDS             9901..10107
                     /locus_tag="PKL60_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95454.1"
                     /translation="MIDTAGKGEGFAAALVAGMCAQALADGWEFVALFADVENLVSNA
                     VFRRISFYPVCDSHQYIFSQPNHA"
     gene            10159..11739
                     /locus_tag="PKL60_04625"
     CDS             10159..11739
                     /locus_tag="PKL60_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNW95455.1"
                     /translation="MRTIGAAEQRVIEEAAARAGVPAGVLMERAGRALAERIMSLLAE
                     RGGGPVSILCGPGNNGGDGYAAARFLTGKIPELHLYEDDSAASAGADAQANRAACRSL
                     GIQVLGYADFNLTSGIVLDAVYGSGFRAERNPKPDFMALSQRVQASRRQFSAYLLSAD
                     IPSGVQADNGAVGAAAFQADETLCFILPKTGVWSYPGRKFAGLISVNDLGLGQSFIDQ
                     VWEDHQFRSPSVLTESEVRAWRPERAPDSHKGSFGRLAIMSGSVGLAGAAVLCARAAQ
                     MGGAGYVDLLVPQEIYPAVLAAVPSVLSQALPADANPRLALWQAKLESCSSALVGPGL
                     GKLASLDLVLAAIAGAPRLVLDADALNLLALPENLKRGRAALLERGNHGLEPAVLTPH
                     PGEFGRLCPEYAQTVNQDRIQSAEALARMTGSVVVLKGAGTVVAFPEEDCKPEIWINS
                     SGNAGMAKAGSGDVLAGLLASFLAQKLPLREAALSAVYLHGLSADLRANELTERALTP
                     EDTLAGLPAAFRQAGWDR"
     gene            11769..13349
                     /locus_tag="PKL60_04630"
     CDS             11769..13349
                     /locus_tag="PKL60_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNW95456.1"
                     /translation="MRLQLHTQADEIQPFLMDESSAAGRAEGIFFPQNEEEIREALKF
                     CRENRKPVTIQGARTGITGGAVPSGGLLINLSRCSRALGMGWDEESQRFTLTVQPGML
                     FSQVQKGLAEKEFNTEGWSAESLRAYRRFGTAGRWHFPADITETSASIGGMLSTNASG
                     AKSYLDGPMRAHVLGLRALLMDGRALVLRRGGQTARDGVFRLRLEDGSAIEGQLPGYR
                     MPRVKNASGYSVAPDMDLLDLFIGAEGTLGVVTEITIALAPLPKLSWGILTFFDEPAL
                     ALRYVQALKAIQGAHRPAAIEYFDRACLEMIHREQVETHAHLGLPLLPPAEAVYAEFR
                     HPDQEEMDLCLHQTAGLLENAGANPAETWLAVTPQDLDKLIGFRHAVPESINRIIADR
                     KREFPTLTKLGTDMAVPDDKLEWVLGLYTESIRALGLEGLKFGHIGDNHLHVNILPRS
                     ELEYQAGKALFLDWARQVIAAGGTVSAEHGIGKLKTAQLLAMYGEQAVAEMRALKRCF
                     DPQGLLNPGNMFTLEGGA"
     gene            13352..14164
                     /locus_tag="PKL60_04635"
     CDS             13352..14164
                     /locus_tag="PKL60_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNW95457.1"
                     /translation="MEIVVCLKQVPASTRVRLNPATNTIVREGVELMLNPFDAYALEE
                     ALRLKENFGGQVTVLSMGIPSAEGILRQALGLGADRAVLLSDKRFAGADTLATARTLA
                     AAVRKLGTVDLLLCGRQAVDGDTAQVGPSLAQKLDIPHLSAVRRVRLLGNRELLCEKM
                     SDEGCLKIRLGLPALLTVVKEINTPRFLNLQGYLQSLRAQVEIWTADDLQLDPARIGL
                     EGSATWVVSTAAADRRKTGKDIPGSPEEAAASILAALRQKGWSKPEREESRG"
     gene            14157..15356
                     /locus_tag="PKL60_04640"
     CDS             14157..15356
                     /locus_tag="PKL60_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HNW95458.1"
                     /translation="MAEIRVVVDNCTGCRKCVPACPYGAISMEGKKARISEACVFCGA
                     CVEVCRFDAVLLRVEEPAGQSEAPESSGIWVFAEQSEGAAKPVALELLGEARRLARKR
                     PGKVSALWLGPETPEQFQKLIAHGADEVLAVTDERLRQRDELLYPQVLARLAEAYKPE
                     IFLFGATGFGRSIAPRTASALDTGLTADCTRLDIDARSGLLLQTRPAFGGNLLATILC
                     RSRRPQMASVRPKVMQALPADDTRQGKVRRESLDLSDMPGLEIVSVRKNTQDGPGLER
                     AEFVVGIGRGIGSARRVGLYRQLAAALGAGLAASRAVVDLGWVDYAHQVGQTGITIAP
                     KVYLAFGISGSVQHMAGLAENTYLIAVNKDGNAPIFSAADERVQADCNEVASALLKQV
                     GGGASLS"
BASE COUNT         3308 a         4390 c         4856 g         2982 t
ORIGIN      
        1 gcagggcgga cgagagtttt aatcctcggt aaaaaacact ggagacctaa aggtcaacct
       61 ccgggcgcgg tcaggagagc ggcgggagct ttgggccggc ttccacgcgg tcaggagagc
      121 ggcgggagct ttggaggagc tttggaaacc agatttcccc cgggaagccc agatcgcctg
      181 tcaaaatcct aaaatcctgc gcacatttgt gaaaataaga tacaataaac ccggaaattc
      241 aagccactct tgcgagtggt tgttaattgc gacccaaacc aaagctccgg gaaaaaacca
      301 gggcaattaa ggagacaacg aatgaaacat caaatggtca tgaccgacgg caacgaggcg
      361 accgcgcatg tcgcttaccg cctgaatgaa gtaatcgcga tttacccgat tacgccgagc
      421 tcggcgatgg gcgaatacgc ggatcagtgg atggcggaac gcgtcccgaa cctgtggggg
      481 accatcccca ccgtggctca aatgcagtcc gagggcggcg cggctggcgc ggtgcacggc
      541 gccttacaag ctggtgcgct ttccacaacc tttaccgcgt cccagggtct gctgctgatg
      601 attccgaaca tgtacaagat tgccggcgaa ctgacctcca cggtattcca tatctccgcg
      661 cgttccctgg cggcgcaggc gctctccatc tttggcgacc accaggatgt gatggcttgc
      721 cgccagaccg gctgggcgat gctctccagc ggctccgttc aggaagcgca ggactgcgcc
      781 ctgatcagca ccgccgccac gctgcgcgcg cgcgtgcctt tcctgcactt tttcgacggt
      841 ttccgcacct cgcacgaggt taacaagatt gaacggatta cggacgaaga actgcgtgct
      901 atggtggatg atgacctgat tatcgagcat cgcaaacgcg cgctgagccc cgatgcgccg
      961 gttgtgcgcg gtaccgccca aaacccggat gtttacttcc agggccgcga gaccgtgaat
     1021 acctactacg acgcctgcgt tggtattgtc tcgcaggaaa tggaaaagtt cggcaaactg
     1081 accggacgtc cttaccatat tatggaatac tgcggcgctc cagacgcgga acgtgtgatg
     1141 attatcatgg gttccggcgc gcaaaccgcc agtgagaccg ctgaactgct cgccaaacag
     1201 ggcgagaaag tcggcgtgat ttacgtgcgc ctctaccgcc cgttctcggt caaacacctg
     1261 atggctgcgc tgcccgcgac ggtgaagaaa atcgctgtga tggaccgcac caaagagtcc
     1321 ggcgcgctcg gcgaaccgct ctatcaggac gtggtcaacg ctgtttctgt cgctatggat
     1381 gagggcactg cgcctttcca gcataagccg gtgattatcg gcgggcgtta cggcttgtcc
     1441 tccaaggact tcacccctgc catggcaaag ggcgtctttg acgagctaaa aaaggaccat
     1501 cccaagcgtc agttcaccgt gggcatcgac gatgatgtca cccacctgag catctcctac
     1561 gacccgagct tcgaggcgct tgggaatgat gtcatccagg ccatgttctt cggtctgggc
     1621 tccgacggta cggttggcgc gaacaagaac tccatcaaga ttatcggcga tgagaccgaa
     1681 aaccacgccc agggctactt cgattacgac tccaagaagt ccggcactgt cacggtttcg
     1741 cacctgcgct ttggccccaa accgattaaa gcgccgtatc tgatttccga aggcgaggct
     1801 acttttgtcg gctgccacca ggtcagcttt gtggaccgct tcgatatgct gaaatacgcc
     1861 cgcaaggggt ctgtcttcct tctgaacagc atctacagcc ctgaagaagt gtgggacacg
     1921 ctgcccaagc agatgcagca ggacctgatt tcgaaagaaa tcgagttcta tgtgatcgac
     1981 gcttattccg tggcggaaaa gaccggcatg ggtcagcgca tcaacaccat tatgcagacc
     2041 tgtttcttcg ctatcagcaa aatcctgccc agggacgaag ctatcgcggc catcaagcat
     2101 tccatcgaaa aaacctacgg caagcgcggc gaagcggtgg tgaacaagaa cttcgcagcc
     2161 gtggaccagg ctatcgcgaa catgcacagg gtgaaagtgc ccgcgcaggt taccagcacc
     2221 atcgagcgcc acagcccggt gcccgcgaac gcgcctgatt atgtgaagga cgtgattggc
     2281 accatcatcg accgcaaggg cgactcgatt aaggtcagcc agatgccggt ggacggcacc
     2341 ttcccgaccg ccacgaccaa gtgggaaaag cgcaacatcg cgctggaaat cccggtatgg
     2401 gaaccggatg tttgtatcca atgcgcgcgc tgctcatttg tgtgcccgca tgccgccatc
     2461 cgcgtcaaga tttacccgac tgagctgctg gcgaacgcgc cggaaggtta caaatccacc
     2521 gcctccaagg cgcgcgaatg gaaagacggc tattcctgga ctgtccaggt agcgcccgaa
     2581 gactgcaccg gctgcggctt gtgcgtccag aactgccccg ggaaggacaa gaccgacccg
     2641 aaccgcaagg cgattaacct ggctccgcag ccgcccatcc gcgaacggga aatgaagaac
     2701 tgggatttct tcctctccct gccgatgctc gaccgcacta ccatccccac tcacctgatc
     2761 aagaactccc aactgctgga accgctgttc gagttctccg gcgcgtgcgc cggctgcggc
     2821 gagaccccct atatcaagct ggccactcag ctctttggcg accgcatggt gattgccaac
     2881 gccaccggct gctcgtccat ttacggcggc aacctgccca ccactcccta ctctaagaat
     2941 cgggatggac gcggtccggc ctggtcgaac tccctgtttg aagataacgc tgaatttggc
     3001 atgggcatgc gcctgaccat cgacaaactg gctgtatacg cccgcgaact gctgggcaaa
     3061 catgccccgg aggtaggcgc tgaactggcg aatgccattc tgagcgccag ccagaagtcc
     3121 gaggctgaaa ttcaggaaca gcgcaaacgc gtacgcgaac tgaaggctgc gctggaaggc
     3181 aagaccgaac aggaactcaa gaacctgctt tccgtcgcgg attaccttgt gcgcaagagt
     3241 gtctggatta tcggcggcga cggttgggcg tatgacatcg gttacggcgg actggaccac
     3301 gttttggctt caggcaagga cgtgaacgtg ctggttctcg atactgaggt gtactccaac
     3361 accggcggtc agtcctccaa ggccaccccg cgcgccgccg tggcgaaatt tgccgcggac
     3421 ggacgcgaca aacccaagaa agacctgggc cttatcagta tgagctacgg gaacatttac
     3481 gtggcgcacg tggcgatggg cagcagcgat aaccagaccc tgagagcgtt tgtggaagcg
     3541 gactcttacg aaggtccgtc gctgattatt gcctacagcc actgcatcaa ccacggcatc
     3601 gatatgtcca agggtttggt acagcagaag ctggcagtga attccggcgc atggccgctt
     3661 taccgctacg acccgcggct ggctgacagc ggaaagaacc cgctgcagct ggactccaag
     3721 gctcccagca tcaaagtgtc cgaatacgcc tacaacgaga cccgctaccg catgctgctg
     3781 cagtccgacg aggaacgcgc ggaaatgctg atgaaagccg ctcaggcgga cgcggaacgg
     3841 cgttggtcgc tctacgaaca gatggcagcc atggactact ccttcggaaa gcccgctgaa
     3901 ggagaggcgc cgcaggcgta ggcattcttt gagatgtgcc ttcggaaagg ataatcgcca
     3961 tcccggcggt tggaagaagg cgcgttaata aggtagaata aacgatgacg tgtttccacg
     4021 cgtcatcgtt ttctattcga ttaccccgag gaggaaagat ggctgaattg aaaacaagct
     4081 acctgggtct gccgttaaag aaccctctgg tggctggcgc gtcacctttt actctgcagc
     4141 ttgacgtcgt caaacaactg gaggacagcg gcgttagcgc cctggtgatg cactccctct
     4201 ttgaggaaga agtcacgcac gagagcctgg aacttgatta tttcctgaac aaaggcaccg
     4261 aaagccaacc cgaagcgcaa acatatctgg tggaaccgcg tagaattgaa agtatcgctg
     4321 acaggtatat cgcccaactg caaaaaatca aggagagcgt tgaaatcccc gtcattgcca
     4381 gcctgaacgg ggtgaccccc ggcggctggg tgcgcctggc gaaagacatg caggcagccg
     4441 gagctgacgc gctcgagctc aatatctaca cgttggtgac cgacccgggg cttaactcgg
     4501 accaggtcga aaagaattac atcagcctta tccaggaagt ctgcaagcag gtgaacatcc
     4561 cggtagcggt caagctttct cctttcctga ccaccctgcc gcattttctg gcgcgggcgt
     4621 ataaggccgg agcggagggg tttgtgctct tcaaccgcta catgcagccc aacttcgaca
     4681 ttgaatcgct ggaggtggag cggaaggccc gactgagcac caacgcggac ctggccctgc
     4741 cggcgcgctg gattgccatt gcttcttccc agttgaaggc cgatttcgcg ctcagcggcg
     4801 gcgtgcacag cccggaagac atggtgaagg ccatcatggc cggcgcgaaa gtggcttacg
     4861 tcgtctcagc actgctggcg aacggggtga aacacgcggc cagactgttg agcggtctga
     4921 gcgtctggat ggacaggaac ggctacgaat ctgtggaaaa aatgctcgga accttacggc
     4981 tggataaaat tgaaaacccc gaggcgtacg agcgcgcaaa ctacttgcgc acgctgaaat
     5041 cctttgatga gcgcgtaata taaacgcgcg taacgattag aacggcttga taagggaatt
     5101 atgggcgttg tcgacgagat taaggcgcag ctcgatttgg tggattatat ctcccagacc
     5161 gtaaaactgc gccgtaccgg caaaaactat attggttttt gccccttcca cagcaacacg
     5221 cgcacgcccg cttttgtcgt cttcccggaa agtcagacct ggcgctgctt tggtcagtgc
     5281 aacgagggcg gggacttgtt cggctttgtc atgaagaaag agggctggga tttcaacgaa
     5341 acccttcgct ttctggcaga aaaagccggc gtcgcgctca gttcgcagcc caggattgcc
     5401 gaagccgatg cccaggcgcg cgatgtcctc ataaaagtgc tggaggaagc cgcgcagttc
     5461 taccgttccc agcttgtgga gagcgaagcc ggcggcgcgg cgctggccta tctgcggcac
     5521 agaggcttgt cggatacgac catcaagatt tggggtttgg gttacgctcc cgccggatgg
     5581 gacgaactga ccaggcagct cacgcgcaaa ggttacacca gggagcacct gctgcgcgcc
     5641 ggtttgctga cagagcgcga ggacggcggc acccatgata aattccgcca ccgcctgatg
     5701 tttcccatcc gtgacaccta cgggaaaatg gccggctttg gcgggcggat tttgaacccg
     5761 gacgatgttc cgaagtacct caactccccg cgcacggagc tttttgacaa agggcgcctg
     5821 ctctacggat tggacctggc gcgtcaggaa atccgcaaag cagagcaggc ggtcatcgtt
     5881 gaaggatata tggacgttat cggtcttcac caggccggct ttggcaacgc cgtctcaccg
     5941 atgggcaccg cgctgacgga agaccagttt aagctgctga agaaattcac gcgcagcatt
     6001 attctggcgc tcgaccccga tgctgccggt gaaaaagcga ctctgcgcgg cctggaaaca
     6061 gcgcgccaga ccatggacca ggacgaccaa ccgactttcg actcacgcgg tttactgcat
     6121 tttgaaggcc gtctgaaggc tgatatccgc gttacgactc tgccagccgg gcaggacccg
     6181 gacgaaatcg cgctggccga cccggaagcc tggcgcaaaa tcgcctccga tgcgaaaccc
     6241 gtggttgtgc acgtgatgga aaccctggct gccgcgcagg acctgaacga cgccaaggtg
     6301 aagcgcgaaa ttgccgccag ggtgctgccg ctgattgagg acgtcgggga cgccgtggaa
     6361 cgggatgcct accgccaaca gttggcgcgc ctgctgagag tggatgaacg ctccctggtg
     6421 cttgccaacc ccgtccaacg ccgtgcccga cgcagcagcg aggagcacgg ccgcagcgag
     6481 cttgaaagca gcttgaatgc catcacggaa aaagcgcgca gcaaccgcat gctggaagag
     6541 caggcgctgc gcacgctcgt gcaagagcct tattctctta atgaaatcaa cagggctctg
     6601 ggtttgatga gcgtcccgca gcttggagat gaggattttg tcgaaagcga tttccgccag
     6661 gcttttcgcg tggtgctgaa cagcatcagg caggaccaac tttctccgca ggattatctt
     6721 gcggaaaacc tgccggattt gttctcctct gaagaaccgg cagcaatgat tgaaaaatcg
     6781 cttccgccca gtgcgcaaaa acgcctggct gaccaggtga gaactgtgct gcgtatcaag
     6841 cgcagccatc tggagacgcg catccaggaa atcctgttcc tgcagtctga attggaagaa
     6901 aagcgctatt ccgcggaaga agctgaagtg ctgctgaccg agttgatcgt ccaacgcaga
     6961 acgctggatg aggctatgaa gtccaaaatg gcaggcggcg atggcatgga agagcgcaaa
     7021 cccaggcaca aaccattaag tatggggaaa tagcgcatgg atgacgattt gcaatttgaa
     7081 gcggaagacg acgaaaaatt cgaagccggc agcgaaccgc acgaaaccct ctctccttcc
     7141 gaggagtaca caccggaaga agagagtgga acccagtcgg atgagcctat tcaactttac
     7201 ctgcgggaaa ttaaccgcag ccgtctgttg gaagctcagg aggaatttca actggcaatt
     7261 tgcgtccagg ccgaaaaaat ccagtccctt tttatcaagc ctgataactc cctgaagctg
     7321 ccaggcctgt acgcgtccat cgccaacctc agcgaaaaga ttactgcagc aagcgcggcg
     7381 ctgaaaaaag aacccctgga cctgtcacag gtgttggctg aggcttccgc gctgcgccgg
     7441 aattttcagg atgcgctgcc ctcctatacc cataattttc ttcaggacga gcgctggaaa
     7501 caggacgccg aatggcaaaa tctggccagg ctgctgtgct cattcctcac ggcggtgtac
     7561 ttgctgccgg tcccattcct gaacagcgtg gcggaactta ctgccaggca tgggcgtttc
     7621 ccttcgtggg aggaactttc cgaaatccaa ccggatccgg atgagatggc gttgagcacc
     7681 cggcaggtgc gcgatattgc gaaattagcc aggcgcgatt tggtggaatt caacctgcgg
     7741 ctggtggtga gcgttgccaa acgctacacc ggcagaggta tcaacattga ggacttgatt
     7801 caggaaggaa atatgggctt gctgcgcgcg gttgaaaaat tcgaccccgc gcgcgggttc
     7861 cgcttcagca cgtacgccac ctggtggatc agacaatcca tcagccgtta tattttggag
     7921 tatgcgcgca ctatccgcat tccggtgcat atggtggaat caatcagcaa actcatcaaa
     7981 gcgcagcata tgctggtgca ggagctgggg cgcgagccta attttgctga aatcgcggct
     8041 gcctcggggc tgctttcgag cgaggacgaa caggaaatcc gtcggaatgc tttctcgcgg
     8101 gaggctttac ccgctgactt gctgcgccgc tgggacgaag ctgccctcaa agtggaacaa
     8161 atcctgaagg ttggcgagga gccggtttcg ctggagagcc cggtagggga tgaagataac
     8221 agcacgctgg gcgactatat agaggacgct gacgcgccgg aaccgatgga ggctgtcatt
     8281 ctggagagcc tgcgggaaag cgtgcgccag tccttgagcg acctgaccga gaaggagcgc
     8341 gcgattttgg agctgcgttt cgggctggtg gatggggtgt accattcgct tgaggaaatc
     8401 agcgagaaat ttggcctgac gcgcgagcgc atccggcaga ttgagagcac cgcgctgaga
     8461 aaattgaggg acccgcgccg gatgaattcg ctgcgggact ttttccagag ttcgtgaggt
     8521 gggtggattg gcggggggct gtcgtctgca ggctggcttg cgctttacaa accggcagcc
     8581 tggtcttggg tgagcatccc aaggaacctg aaaggggtgc gaatgcaagc aataaaaagg
     8641 ggccgtccga aaagccggat ggccctactt aatttccctc tccacgcccg ttttcatagg
     8701 cccttacgga tttgcgctat caaaacgagg cctatacagg cacgttctcc cactttgggc
     8761 tggctaaaaa ggaacccccg ttctttttgg acagcccctt tccagcttct atctccgacc
     8821 gcggcagccg cgcatcgggc ccctgaatgc ctggtaggtc tccaactgtt cggcggtcat
     8881 cacctggggt tcgtacaccg cgttggtcat cttctcccac ccgcgcacga acgcgcgcag
     8941 gtgcatgctg gagccttgca gcagccggcg gtaggcgcgc tgcacgtccg cgtgcgtgct
     9001 ttcatcgatt gccttctcga ggtcaagaat atccctttcc tcgatatagc cgccgacttt
     9061 cagcgcttct tcctcggaaa gcgagccctt cgcgaccagg tcatcataaa gctggtcgaa
     9121 ttgcgggtcc ccatactcac cggctcccaa ttggtccagg ttcgaattga tgccgtagtc
     9181 tcccaaaagc tggaacatgc ggtcgaagtg ccgggtctcg ctgcgcgcga tacgctggaa
     9241 ggcggcgagt tcccactttt cgtacagcgc cagatagacg tcgtgtgcca gcttttcctc
     9301 cgcggccatg tgcaggaggg tgctgcgttc cgcctcgctt agagtttccg ccgaatcggt
     9361 ctgggcgctc accggcgtcg ttatcgcgcc gattcccacg aaggcgagta cgacagccaa
     9421 actgagcagc aaaatcctgg gaatagtttt cattgttcac tccatttcgt tataatgagg
     9481 gaaatcctct gttccaaaat atagcggaca tttattaggt atctattaag cggctgtgaa
     9541 gaaattgtga agatgctcaa ttcctccaga aaggcggcac aatgacggaa aaaccggcaa
     9601 aacacggaga ggatcaggac ggactgcctc tgtatcagcg cgtttacgcg cttgtcaagc
     9661 aaatcccggc cggcaaagtg gcaacctacg gccaaatcgc gcgcatgctg gggggctgtt
     9721 cggcgcggat ggtgggttac gctatgagcg cgctgcgaga cggacgcgat ccggaagtcc
     9781 cctggcagcg ggtcatcaac gcgcaggggc gtatcagcct gcacggtatc ggctcggctg
     9841 aacagcgttt tttgctggag gacgaaggcg tggtatttga cgagggcgac cgtgtcagtt
     9901 ttgatagata cggctgggaa gggtgagggc ttcgcagccg cgctggttgc tggaatgtgc
     9961 gcgcaggcgc tggctgacgg atgggaattt gtcgcgctct tcgccgacgt ggagaacctg
    10021 gtcagcaacg cggttttccg acgcatcagc ttttacccgg tgtgcgattc ccatcaatac
    10081 atttttagcc agcccaatca tgcgtaaaac agccggggag cgccgcgcaa tgacaaacct
    10141 ggagcgggag caaagaacat gagaactatc ggagcagcag aacaacgcgt gatcgaagaa
    10201 gcagccgcac gggcaggcgt tccggcaggc gttttgatgg aacgtgccgg gcgcgcgctg
    10261 gcggagcgga ttatgagcct gcttgctgag cgcggcgggg gacccgtcag catcctgtgc
    10321 ggcccgggca ataacggcgg cgatggctat gccgcagccc gttttttgac cggaaaaatc
    10381 cctgagctgc atctctacga ggacgacagc gccgccagcg caggggcaga cgcgcaggca
    10441 aaccgcgccg cctgcagatc acttgggatt caggttttgg gatacgcgga tttcaatctc
    10501 acttccggga ttgttctgga tgcggtatac ggcagcggtt tccgcgcgga gcgaaacccg
    10561 aagccggatt tcatggcgct cagccaacga gtgcaggctt cccgccggca gtttagcgcg
    10621 tacttgctta gcgcggatat cccttccggg gtgcaggcgg ataacggcgc cgtcggcgcg
    10681 gcagcattcc aggcggatga gaccctgtgc ttcatcctgc caaaaaccgg cgtctggagc
    10741 tacccggggc gaaagtttgc cggcttgatc agcgtgaacg accttgggtt gggtcaaagt
    10801 ttcatcgacc aggtttggga agaccatcag tttcgcagcc cctctgtgtt gacggaaagc
    10861 gaggtgcgcg cctggcggcc tgagcgcgcg ccggacagcc acaagggcag cttcggcagg
    10921 ttggcaatca tgtcaggcag tgtgggcttg gcgggcgcgg ctgtgctttg cgcgcgcgcg
    10981 gcgcaaatgg gcggggcggg ctatgttgac ctgctcgtgc cgcaggaaat ttacccggct
    11041 gtgcttgccg ccgtaccgtc cgtgctcagc caggctctgc ccgcggatgc gaacccgagg
    11101 ctggcccttt ggcaggcgaa acttgaaagc tgcagctccg cgctggtggg gcccgggctg
    11161 gggaagctcg caagccttga tttggttctg gcggccatcg ccggcgcgcc gaggctggta
    11221 ttggacgcgg acgcgctgaa cctgctggcg ctgccggaga acctgaagcg cggacgggcg
    11281 gctttgctcg agcggggaaa tcacggtctg gagcccgcgg tactgacccc gcacccgggc
    11341 gaatttggcc ggctttgccc ggaatacgcg caaaccgtga atcaggaccg catccaaagc
    11401 gcggaggcgt tggcgcggat gaccggcagt gtggtggtgt tgaagggcgc gggcacggtg
    11461 gtggcttttc cggaagagga ctgcaagccc gaaatatgga ttaacagcag cggcaacgcc
    11521 ggtatggcca aagccggctc gggcgacgtg cttgcggggc tgctggcttc gttcctggcg
    11581 cagaaactgc ctttgcgcga ggcggcgctc tccgcggtgt acctgcacgg gctttcggcg
    11641 gatttacgcg ccaatgaact gaccgaacgc gcgctcacgc ctgaggacac gctcgcgggg
    11701 ctgccggcgg ctttccgcca ggctggctgg gaccgctaac cgccggtttt tcgcttacaa
    11761 ttgcagccat gcgcttacaa ctccacaccc aagcggatga aattcagcct ttcctcatgg
    11821 atgaatccag cgcggctggc cgggcggagg ggattttttt cccgcaaaat gaagaggaaa
    11881 tccgcgaagc tctaaagttc tgccgggaaa accgcaagcc cgtcaccatt cagggcgcac
    11941 gcaccggcat caccggcggg gcggtccctt cgggcgggct gctcattaac ctgagccgct
    12001 gcagccgcgc gttggggatg ggctgggatg aagaaagtca gcgctttacg ctgacggttc
    12061 aacccggcat gctgttcagc caggttcaaa aaggcctggc ggaaaaagaa ttcaacacgg
    12121 aaggttggag cgcggaatcc ttgcgcgcct accggcgctt cggcacggcc gggcgctggc
    12181 acttcccagc ggatatcacc gagacctccg cttcgattgg gggcatgctt tccaccaacg
    12241 cttccggcgc caaatcctac ctggacgggc caatgcgcgc gcacgtcttg ggtctgcggg
    12301 cgctgctgat ggacgggcgg gcattagtgc tgcgccgcgg cgggcaaacc gcgcgggatg
    12361 gggtttttcg cttgcgcctg gaggacggca gcgccatcga gggccaactt cccggttacc
    12421 gtatgccgcg cgtgaagaac gcgtcggggt attccgtcgc gccggatatg gaccttctgg
    12481 acctgttcat tggcgcggaa gggacgctcg gcgtcgtcac cgaaatcacc atcgcgctgg
    12541 ccccgctgcc aaagctgagc tggggaatcc tgacattttt tgatgaacca gcgctggcgt
    12601 tgagatatgt acaggcgctt aaggcaattc agggggcgca caggccggcc gccatcgagt
    12661 attttgaccg cgcctgcctg gagatgatcc accgcgaaca ggtggaaacg cacgcccacc
    12721 tgggcttacc cttgctgccg ccagccgagg cggtttacgc ggaattccgc caccctgacc
    12781 aggaggagat ggacctttgc ctgcaccaga cagccggatt gctcgagaac gccggcgcaa
    12841 acccggccga aacctggctg gctgtgaccc cgcaagacct ggataagctg ataggcttca
    12901 ggcatgccgt gcctgagagt atcaaccgca tcatcgccga ccgcaaacgc gagtttccga
    12961 ccctcaccaa gctgggcaca gacatggccg tacccgacga caaactggaa tgggtgctgg
    13021 ggctctatac agaatctatc cgcgcgctcg ggttggaagg gctcaagttc gggcatatcg
    13081 gggataacca cctgcatgtc aacatcctgc cccgcagcga gctggagtat caagcgggca
    13141 aggcgctctt tttggactgg gcccgccagg tcatcgccgc gggcggaacg gtctcggcgg
    13201 aacacggcat cggcaaactg aagactgccc aactgctggc aatgtacggg gagcaagccg
    13261 tggcggaaat gcgtgcgctg aagcgctgct ttgacccgca ggggctcttg aaccccggca
    13321 atatgttcac gctggaaggc ggcgcgtgag tatggagatt gtggtgtgcc tcaagcaggt
    13381 gccggccagc acgcgcgttc gcttgaaccc ggccacgaac acgattgtgc gcgagggcgt
    13441 tgagctgatg ctgaacccgt tcgacgcgta cgctctggaa gaagccttgc gcctgaagga
    13501 aaactttggc gggcaggtca cggtgctgag catgggcatc cccagcgcgg aaggaatttt
    13561 gcggcaggcg cttggtttgg gggcggaccg ggctgtgctg ctcagcgaca aacgcttcgc
    13621 cggcgcggac acgctggcta ccgcccgcac gctggcggcg gctgttcgta agctggggac
    13681 ggttgacctg ctgctgtgcg gcaggcaggc tgtggacgga gataccgcac aggtagggcc
    13741 ttctttggcg caaaagctgg atatcccgca tctgagcgcg gtcaggcggg tgcggttgct
    13801 gggcaatcgg gaactgctgt gtgagaagat gagtgacgag ggctgcctga agatacggct
    13861 tggtttgccg gcgctgctga cagtggtcaa ggaaattaac acgccgcgtt ttctgaattt
    13921 gcagggatat ctgcagtcgc tccgtgcgca ggttgagatt tggacggcgg atgacctgca
    13981 gcttgatccc gcgcggattg gactggaggg ctcggcaacc tgggtggttt ccaccgcggc
    14041 tgcagaccgc cgcaaaaccg gcaaggatat tcccggttcg cccgaggaag ccgccgcttc
    14101 catccttgct gcgctcaggc agaagggctg gagcaagccc gaacgcgagg agagccgtgg
    14161 ctgagattcg cgttgtggtg gataactgca ccggctgccg caaatgcgtg cctgcctgcc
    14221 cctacggcgc catcagcatg gaaggtaaaa aggcacgaat ctcggaagct tgcgtcttct
    14281 gcggggcttg cgtggaagtt tgccgctttg atgcggtttt gctgcgggtt gaagaaccgg
    14341 ccgggcagag cgaggcgcct gaaagcagcg gcatttgggt attcgcggaa cagagcgaag
    14401 gggctgccaa accggtggcg ctggaactgc tgggagaggc gcggcgcctg gcgcggaaaa
    14461 ggccgggaaa ggtcagcgcg ctgtggttgg gcccggagac gccggaacag tttcagaagc
    14521 tcattgcgca cggcgcggat gaggtgctgg cggtcacgga cgagcgcctg aggcagcgcg
    14581 acgagctgtt gtacccgcag gtattggcgc ggctcgcgga ggcgtataaa cccgagattt
    14641 ttctatttgg cgcgactggc ttcgggcgtt ccattgcccc gcgcacggct tcagcgctgg
    14701 ataccgggct gactgctgac tgcacccggc tggatatcga cgcgcgcagc ggtttgctgc
    14761 tgcagacccg gccggcattt gggggcaacc tgctggccac tatcctttgc cgcagccgcc
    14821 gcccccagat ggccagcgtg cgcccgaaag tgatgcaggc tttgccagcg gatgacaccc
    14881 ggcaaggaaa agtacgccgt gaatccctgg atttgagcga catgcccggg ctggagattg
    14941 tatccgttcg gaaaaacacg caggacggac cgggattgga gagagccgaa tttgtggtgg
    15001 gcatcggccg cgggattggc agcgcgcgcc gggtggggct ctaccgccag ttggcggctg
    15061 cgttgggggc gggattggcg gcttcgcgcg cggtggtgga cctgggctgg gtcgattacg
    15121 cgcaccaggt cgggcaaacg ggcatcacga ttgccccgaa ggtttacctg gcgtttggca
    15181 tttcggggtc agtgcagcac atggctgggc tggcggagaa cacgtatctc atcgcggtga
    15241 acaaagatgg gaacgcgcca attttttccg cggcggatga acgcgtgcag gcggactgca
    15301 atgaggtagc ctcagcgctg ctgaaacagg ttggcggtgg cgcatcctta tcctgagcaa
    15361 caccgaagga tattttcagc ttaaaaccag attcttcact tcgttcagtt cgtaggttgg
    15421 gtgaagcggc aggaaagtcg taggtcgggt ggaacgtcag cgcaacccga cagaaagtgg
    15481 ccgtaggtcg ggtggaacgt cagcgcaacc cgacagaaag tggccgtagg tcgggt
//
LOCUS       DAOQAD010000039        14445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_4555_length_14445_cov_5.838985, whole genome shotgun sequence.
ACCESSION   DAOQAD010000039 DAOQAD010000000
VERSION     DAOQAD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14445
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4555_length_14445_cov_5.838985"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            429..620
                     /locus_tag="PKL60_04645"
     CDS             429..620
                     /locus_tag="PKL60_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95459.1"
                     /translation="MDDNTRPKNEAPQPDAMGSMDFLTLNMLGLSEAELRDLLSPEEL
                     AAYRAFLRRDAADLDSGGL"
     gene            826..1620
                     /locus_tag="PKL60_04650"
     CDS             826..1620
                     /locus_tag="PKL60_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW95460.1"
                     /translation="MKKSLVFLVLVVLTAALVAGCAGQKAESVLDKIKTAGKVVVGTS
                     ADYEPYEYVDEAGNFTGFDIELMTEIAKRMGVELEWTDMPFDSLIAAVQEGKIDMSIS
                     CFNYSEERDAQVDFSDAYYTSEDAFLVTEAFAGTIADPLEVAQYKIGVQSGTVQDDWV
                     TTELVDTGLMPESNLSRYERVDQAALDLQAGRIDVLVADSVPAKAIMQQQAGLKIVYE
                     GMLYTGPINIVLPDGDTALQAEVNRIIKELQDEGFIDALAVKYFSE"
     gene            1705..2349
                     /locus_tag="PKL60_04655"
     CDS             1705..2349
                     /locus_tag="PKL60_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNW95461.1"
                     /translation="MDFSRFIQYIASGTAYTLGVTLIALPSGLLIGLVLAFVAVYGGK
                     LAGKIMDVYSTVLRGIPPIVLLFILYFMMSGAINLSPFWAGSISLGIISSAYQLEIFR
                     GAILSVSGEQMMAARAVGMSKIQAIRYVVLPQMIRLAIPSWSNEAAIVIKDSSLVYAI
                     GVPEILRRAQLISASTQEPFLAYITAALIYFALVFITNRVLGRVERKLTIPANP"
     gene            2365..3129
                     /locus_tag="PKL60_04660"
     CDS             2365..3129
                     /locus_tag="PKL60_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNW95462.1"
                     /translation="MSKPILTVKQLTKRYGSNTVFENIDLTVNEGDTIVIIGPSGTGK
                     STLLRCLNLLTKPDGGQIWLDDQEITAKGTDEDHVRQHIGMVFQNFLLFNHLTALENV
                     KLGLVRVKHMPEKEAETKALEELARVGLADRAGHYPGQLSGGQQQRVGIARALAMDPR
                     VMLFDEPTSALDPELIGEVLEVMRKLALEGMTMVVVTHEMGFAREVADRIIFMEKGSI
                     VEEGSPDALFYHPKFERTREFLWKITELYGKKEAQA"
     gene            3129..3806
                     /locus_tag="PKL60_04665"
     CDS             3129..3806
                     /locus_tag="PKL60_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNW95463.1"
                     /translation="MEWLQLLQRFFSDFVRGAGVTLELTAVGLTLGFVLGLVLALAKI
                     YAPRWLSAIATAYIEVFRGTPLLVQLFLIYYGLPSLGITLSQTLSAYLALGLNSAAYQ
                     AEYLRGAIQAIGPSQMMAGRSIGLSRWQTIRFIILPQALRLALPSWSNEPISLLKTTA
                     VVFLIAVQDLMAKAKRAATITYNPIASYLAVAAVYLLMVFALNALLKWLERKTKIPGF
                     DIDVKRV"
     gene            complement(3992..4456)
                     /locus_tag="PKL60_04670"
     CDS             complement(3992..4456)
                     /locus_tag="PKL60_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95464.1"
                     /translation="MPIADDLSHYASIVSWKVKQQTQILHVQAQIHELESQIGIQKTV
                     LGEQTYDLFSQGALTEEPIRQICEKITELMAQKAERQKELELLRSQTPPDKPQSAAPE
                     EAAPAQPQEGAGEWVAPENVSEPAPPAEPGEQSGEWVEPGINTAPPTAPTEE"
     gene            4619..5401
                     /locus_tag="PKL60_04675"
     CDS             4619..5401
                     /locus_tag="PKL60_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNW95465.1"
                     /translation="MALLRIDHSSVCVKVNLPLYIILPSPASLKDKSLADFRVLYLLH
                     GLSDDASAWQRYTNIEILAREQDLAVVMPTGGRSMYTDMNNGQAYFSYLTEELPEYLR
                     KLYRLNLTRENTAIAGLSMGGYGAFKAALLRPELYCAAGSFSGLLGLQLLSLIDDIDL
                     RQEFTQLFGDLNRASGSPDDPLVWLENAAKNPGAFPRLYASCGRQDPLLPINQFFQQR
                     AQSLGFPLTYVEEDGIHDWFFWQSQITHFLRHFYPARPEAAA"
     gene            5474..6544
                     /gene="selD"
                     /locus_tag="PKL60_04680"
     CDS             5474..6544
                     /gene="selD"
                     /locus_tag="PKL60_04680"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5543..5545,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNW95466.1"
                     /translation="MDATDIPNSGKKIRLTSLSSCAGUASKLNAETLAQVLRPLSTVF
                     PTADYPDLLVGLDSADDAAVWKLDETRALVLTTDFFTPVVDDPYDYGAISAANSLSDV
                     YAMGGKPFLALNIAALPPDLPVEVSTEIMRGAAEKSREAGVVIAGGHTIQDKEPKFGL
                     AVLGMLQTDALLKKGGLQAGDHLFLTKPLGFGVSTTAHKRGLLSEDELAEVVGWMKTL
                     NRAAGELAVLSGLHSATDITGFGLLGHLSEMTKASGCGATLRMSAVPLVSCALKYAQA
                     YTFPGGASDNRLYFEPGVHFAGEIPEHERMLLFDPQTSGGLLLAVPKDKLSAFQQNAQ
                     AAALPVWEIGTAESEPGIRVLA"
     gene            6692..7765
                     /locus_tag="PKL60_04685"
     CDS             6692..7765
                     /locus_tag="PKL60_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNW95467.1"
                     /translation="MEKRVTSHDVAELAGVSRTTVSFVFNDDKQYFVRPETRQKVREA
                     ARKLSYYPNASARALASNQAKAIGLIMTRDPHYIAADNFLPQIIGGLLDVVKEKQISL
                     LIEWVEPGEQTHTYLSLTQAKHIDGMILLTPRFDDPGLKALEQLGIPCVIMGQVPGSN
                     LAFVDIDNCAAAHVAVNHLIELGHSRIACITNARLSYSSASQRLLGYRQALVDAGLDM
                     DEKLIREADFDSASGYTAMRSLLSSGSNFTALFAGSDTVALGAMAAIQEAGLTIPGDI
                     SIVGFDDIPEAAFYYPGLTSVRVPANEIARQSCYLLMHLMKGEAPASTSIMLPTELIS
                     RQSTCEIERSHKHQQGEKFVHFT"
     gene            7796..9172
                     /locus_tag="PKL60_04690"
     CDS             7796..9172
                     /locus_tag="PKL60_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW95468.1"
                     /translation="MRSKKQMIWASIAILLVASLVLAACGKTAEAPAAEAPAGETVAA
                     LPQGQELANAYAGQYKGTVVTMAGPFTDQDEVVFNDSIKGFEEKTGIDIQYEGSKEFE
                     ANINIRLDGGDRPDIVDYPQPGLLKYAVEKGYAIDMTTLINPEWIKTNYSDAWQALSK
                     MTGPNGEIVAGIWARANGKSFVFYPKKAFDAAGYKVPETWDEMMALTQQIADDGDTAW
                     CIGIESGAATGWTMTDWIEDVMLRVAGGEKYDQWVAGELKFDSPEVREALDYIEAIWF
                     NDAYVYGGRAAIPTINFGDAPKPMFEDPPKCWFNRQGNFVTTFFPEEAVAGVDYSFFY
                     LPGIKEEYGKPVLGAGDIYAVFNDRPEVRAVIQYFSTGESLKVWVESGGAILTHNDAS
                     LDWYVDPVTRGVAETIRNATTFRFDGSDMMPGAVGAGTFWKYMTDYVSGSITRDEALR
                     QIDASWPK"
     gene            9268..10404
                     /locus_tag="PKL60_04695"
     CDS             9268..10404
                     /locus_tag="PKL60_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNW95469.1"
                     /translation="MNISQTKSPGANNPITKILSWFGTAIGQLFVSVLVPAVTFLVLW
                     QGYLFLQRAAAPQIVQVLVAIVWGVGGVALLYVVTNWLVMKLPKKISKTLQPFVFVGP
                     AVVIMGWFLAVPVVRSLVASFRNYLGTEWVGLENYKFAFTDPRMLETFRNNLLWMVFG
                     TFFSVAFGLLIATLADRSKAEVLYKSIIFTPYALSFVGAGVIWKFIYTYKGTGIGIRE
                     IGLLNAVVTALGGTPQPWLNLTPWNNFFLIVIMIWLQTGYAMVILSSAIKGVPAELLE
                     AARIDGANEFKIFFSIIVPYIKGTLLTVTTTIVIFSLKTFDIVRVMTGGNNGTNVIAN
                     EFYLQSFTYNNAGRASAIAIVLLLLVTPVIINNVRSFNQQRRGF"
     gene            10404..11504
                     /locus_tag="PKL60_04700"
     CDS             10404..11504
                     /locus_tag="PKL60_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNW95470.1"
                     /translation="MANSLKSHKASQSFWTNFVLIVICVLWMIPILGILITSFRPSDE
                     IFRNGWWNVFPHKEDVEVSRVVIPESVDVDGPISYGGKTATFQEWQRGIQLDDGTKAT
                     WYGNKRTRTLIISENKWVGFATNLTLENYKDVLTGKTITYKDAQGQTITRTGNNLGGA
                     FLNSLAVTIPGTIIPILIAAFAAFGFAWLDFPGRNVVFTIIVALLVVPLQIALVPILK
                     DYVKLELNGTFLGIWLAHTGFGLPLATYLLFNYISTIPRELLESAYIDGASNFTIFTN
                     LILPLSVPALASFAIFQFLWLWNDYLVALVFLGEKNQTVTIAVAQLVGEKGQDWHLMT
                     AAAFISMILPLVVFLSLQRYFVRGLMAGSVKG"
     gene            11704..13395
                     /gene="treS"
                     /locus_tag="PKL60_04705"
     CDS             11704..13395
                     /gene="treS"
                     /locus_tag="PKL60_04705"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HNW95471.1"
                     /translation="MTENALWYKNDIFYELYVRAFRDSNCDGWGDLKGVAEKLDYLSY
                     LGVDTIWLLPISPSPLRDDGYDVSDYCGIHPMYGNMQDFQDLVVQAHQRSLKILVELV
                     PNHTSDQHPWFQASRDPMHPEHEKYRDYYVWSDTDQKYQDARIIFVDSEKSNWTYDTL
                     RKQYFWHRFFSHQPDLNYDNPAVQEAMLQTVKFWIDQGADGIRVDAPPYLFEREGTNC
                     ENLPETHLFLKRLRAFVDQYKPGTLLLSEANQWPEDVIHYFGSGDEMHMNFHFPLMPR
                     LYMALARGDRSPIEEILARTPELPPSCQWGTFLRCHDELTLEMVTPEERQWMWDFYAP
                     DKRMRLNMGIRRRLAPLLGNDKRKILLMYSLLLTMGGSPFLYYGDEIGMGDNIWLEDR
                     NGVRTPMQWEDSPNGGFSEAPAEKFYAPVISDPEYGCARVNAAAQMADPDSLLNHIRH
                     LIAVRKQHALFGWGDFAWREFSYNPKTVAAYQRKFEGQRVVVLNNLTDAEVHGWIPET
                     AAAFEDLLTGEQVLPGDYFLPPFGFVWLKPIGPDLPNQPLGPNAPRPQYLGSDKE"
     gene            13524..14435
                     /locus_tag="PKL60_04710"
     CDS             13524..14435
                     /locus_tag="PKL60_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNW95472.1"
                     /translation="MKTPIYGGIEGGGTKFVCAVGSADGESLAETRFPTTTPEETLSR
                     AIVFFQEQTRALGPLAGLGIACFGPLDPDIHSPTYGRILATPKAGWAGADIVGALLPA
                     LAVPIAFDTDVNGAALGEWRWGAAQGLSTFLYFTVGTGVGGGAMVEGRLLHGLIHPEM
                     GHIPLPHNLAQDPFPGGCPFHGDCFEGLASGPAMEKRWGQRAESLPPEHPAWDLEAHY
                     IARAMQGFICSFSPQRIILGGGVPQQPALLPLVRAKTLEYLNHYVNSEAILSHIDSYI
                     VPPGLGARAGVCGAFALAQQAAAGVGL"
BASE COUNT         3122 a         4193 c         3990 g         3140 t
ORIGIN      
        1 agctctcacc atgagagccg ccagagtata tcacatttcc cgcgctggca aggggcaatg
       61 gaggtgatgt gtaagagcaa catgataatg tcctaaagta gcaaaataga aatgtcctga
      121 ttaaaattag gagctatgac atggaccatc gcaatgagca gtaaagaact agcccgcaaa
      181 accgcagtgg aacgggtttt agatcgtcgg agtacccaaa agaaagccgc ggaattgaaa
      241 cgtccagacg cgaacgcctg cctgctccgc gccaactcgc cccacctcca ttccgttata
      301 atcagcatac atgctggtga gagaccatgc cgcagtacct gaaggactac atctcgcaaa
      361 aagacgtgct gcgagagatt gaaatggtcc agcggcagat tgaaaatgac gaagaggatt
      421 gagccgccgt ggatgataac acccgcccca aaaacgaagc cccgcagccg gatgcgatgg
      481 gcagcatgga ctttctgacc ctgaacatgc tcggcctgag cgaggcggag ctgcgcgacc
      541 tgctcagccc ggaagagctt gcggcatacc gcgcgttcct gcgcagagat gccgccgacc
      601 tggattcggg cggcctctaa gcgccttgcc ccttttttgc gccaaaatct gcgctcctta
      661 agataagcac gagaaagaaa tgggcgtccg tggttagcga acccagcgct ttccctcgat
      721 tccgcgcaat ccccgaaaat ctcttaggaa aaatgatgtt ttcagcgttt cacaggtata
      781 attgctttac cttttcatcc ccatatcact tctaggagga agcgcatgaa gaagagtctg
      841 gtttttctgg tgctggttgt cctgacagct gccttggtcg ctggctgcgc ggggcaaaaa
      901 gcagaatccg tgcttgacaa gattaaaacc gccggtaagg tggttgtcgg cacctccgct
      961 gattatgaac cttatgaata tgttgatgaa gcggggaatt ttaccggctt tgacatcgaa
     1021 ttgatgaccg aaatcgccaa gcggatgggc gtggagctgg aatggaccga catgcccttc
     1081 gacagcctga tcgccgcggt gcaggaaggc aagattgaca tgtccatctc ctgcttcaac
     1141 tactccgaag aacgcgacgc ccaggtggac ttctcggacg cttattacac ctcggaggac
     1201 gccttcctgg tgaccgaagc ctttgccggc acgattgccg acccgctgga agtggcgcaa
     1261 tacaagattg gcgtacaatc cggcacggta caggacgact gggttaccac ggaactggtg
     1321 gacaccggcc tgatgcccga aagcaacctt tcgcgttacg agcgcgtgga ccaagccgcc
     1381 cttgacttgc aggccggccg catcgacgtg ctggtggccg attcggtgcc cgcaaaggca
     1441 attatgcagc agcaagccgg cctcaaaatc gtttacgagg gcatgctcta taccggcccc
     1501 atcaacattg tcctccctga tggggatacc gccctgcagg ctgaagtcaa tcggattatc
     1561 aaagagctcc aggatgaagg attcatcgac gctctggctg taaagtactt tagcgagtaa
     1621 gcacattcaa aaaggggata tccagcgcgg gtatcccctt tgcttacctt tcggtaaaag
     1681 cccggacccg gaggttctca gccggtggat tttagtcggt ttattcagta catcgcctca
     1741 ggcactgcct ataccctcgg cgttacgctc atcgcgcttc catccggcct gctgattggt
     1801 ttggtgctgg ctttcgtggc ggtttacggg ggcaaactgg caggcaaaat catggacgtt
     1861 tacagcacgg tcctgcgcgg tattccccca attgtgctgc ttttcatcct ctatttcatg
     1921 atgtccggcg cgataaacct ctcgccattc tgggcaggtt ccatttcgct cggcatcatc
     1981 agcagcgcct accaactgga gattttccgc ggcgcgattc tctcggtcag cggagaacaa
     2041 atgatggctg cccgcgcggt aggtatgtcc aaaatacagg ctatccgcta tgtggtgctt
     2101 ccccagatga tccggctggc aattccctcc tggtcgaacg aagcagcgat tgttatcaaa
     2161 gactcgtccc tggtctatgc gattggcgtg cccgagattc tacgccgggc gcagctcatc
     2221 agcgccagca cccaggaacc tttcctggca tatatcaccg ccgcgctgat ttacttcgcg
     2281 ctggtattca ttaccaaccg cgttttgggg cgggtggaac gcaagctgac cattcccgcc
     2341 aatccttaac ccccggagcc tccgatgagc aaacccatac tcactgtcaa gcagctgaca
     2401 aaacgctacg gcagcaacac tgttttcgag aacatcgacc tgaccgtcaa cgaaggcgac
     2461 accatcgtca tcatcggtcc cagcggaacc ggaaagagca ccctgctgcg ctgcctgaac
     2521 ttgctgacca aaccggacgg cgggcaaatc tggttggatg accaggaaat cactgccaag
     2581 ggcacggatg aagaccatgt gcgccaacat atcggcatgg tcttccagaa ctttttgctt
     2641 ttcaaccacc tgaccgccct ggaaaacgtc aaactgggcc tcgtgcgcgt gaagcacatg
     2701 cccgaaaaag aagcggaaac caaagcgctg gaggaactcg cgcgggtcgg cctggcagac
     2761 cgggcggggc attatcccgg gcagctctcc ggcggtcagc agcagcgcgt gggaatcgcc
     2821 cgcgccctgg cgatggaccc gcgcgtaatg ctcttcgacg aacctacttc agcgctggac
     2881 cccgaactaa ttggcgaggt gttggaagta atgcgtaagc tggcgctgga aggcatgacc
     2941 atggtggtgg taacccacga gatgggcttc gcgcgcgagg tggcggaccg catcatcttt
     3001 atggaaaaag ggagcattgt cgaagaaggc tcgcccgacg cgctgttcta tcacccgaaa
     3061 tttgaacgca cccgtgagtt cctgtggaaa atcaccgaac tctacgggaa gaaagaagca
     3121 caagcctgat ggaatggctg cagctcctgc agaggttttt tagcgatttt gtgcgcggcg
     3181 ccggcgtaac gttggaattg acggcggtcg ggctcacgct gggcttcgtg ctcgggttgg
     3241 tcctggcgct ggcaaagatt tatgccccgc gctggttgag cgccatcgcc acagcctata
     3301 ttgaggtttt ccgcggcacc cccctgctgg tccagctctt cctgatttat tacggcctgc
     3361 cctccctggg tatcaccctt tcccagacgc tctccgcgta tctcgcgctg ggattgaaca
     3421 gcgcggcata ccaggcggaa tacctgcgcg gagccatcca ggccatcggt ccatcgcaaa
     3481 tgatggccgg ccgctccata ggcctctcgc gctggcagac cattcgcttc atcattctgc
     3541 cgcaagcgct caggctggcg ctgccttcct ggtcgaatga gcctatttcg ctgctcaaga
     3601 ccaccgcggt ggtattcctg attgccgtgc aggatttgat ggcgaaagcc aagcgcgccg
     3661 cgaccatcac ctacaacccg attgccagct acctggcggt ggcagcggtg tacttgctga
     3721 tggtattcgc cctgaacgcg ctgttaaagt ggctggaacg caagaccaaa atcccaggct
     3781 ttgacattga tgtgaaacgc gtttgaaata ggcgcggcac gccgtctttt cctgattttc
     3841 ccgcggattc cctgcccgac tggcctgtcc gagcgtactt ccaggcaaag gatcagatcc
     3901 gaccctgggt cgtttgcagg gaaaagtctt aatgaacaac agcgcccgcg gtttacgggc
     3961 gtggttgttt aggctcctct cgcgcgaggc gctattcttc cgtcggagct gtcggagggg
     4021 ctgtgttgat gccgggttcc acccactccc cgctctgttc cccaggctcg gctggcgggg
     4081 caggctcgct cacattctcc ggtgcgaccc actcgccagc accttcctgc ggctgcgcgg
     4141 gcgcggcttc ttccggggcg gcgctttgcg gcttatccgg cggagtctgc gaacggagca
     4201 gttccaactc tttttggcgt tcggcttttt gggccatcag ttcggtgatt ttttcgcaga
     4261 tttggcggat cggttcttcg gtaagggcgc cctgggagaa caagtcatag gtttgttcac
     4321 ccagcacggt tttctggatg ccaatctggc tttccagctc gtgaatctgc gcctggacat
     4381 gcaggatttg tgtctgttgt ttaaccttcc atgagacaat cgacgcgtaa tgggaaagat
     4441 catcagcaat tggcattttc ttttcctttc ttgttcagcc agtgaatgta tcatatcgct
     4501 tttttaggtt tctggcgcgg cgtgtgaaag ctgcttcctg gtttgctttg ggtataatcg
     4561 atgcaaatgg atgccctcaa aaccttacat catccattca aaaaaggtca ggtgacaaat
     4621 ggctcttctc cgtattgacc attcctctgt atgcgttaag gtcaatcttc cgctttacat
     4681 aatcctcccc agcccggcca gcctgaaaga taagtccctg gcggatttcc gggtccttta
     4741 cctgctgcac ggcctctccg atgacgcgag cgcctggcag cgctatacca atatcgaaat
     4801 cctggcgcgg gagcaggacc tggcagtggt gatgcccaca ggcgggcgca gcatgtacac
     4861 cgatatgaac aatgggcagg cttatttcag ctatctgact gaagaactgc cggaatacct
     4921 ccggaagctt taccgcctga acctgacgcg cgagaatact gccatcgcgg ggctctccat
     4981 gggcggatac ggcgctttca aagccgcgct gctgcgtccg gagctgtatt gcgcggcagg
     5041 cagcttttca gggctgctgg gcttacaatt gctttccttg attgatgata tcgacttgcg
     5101 ccaggaattt acccagcttt ttggcgacct gaaccgcgcg tcaggcagcc ccgacgaccc
     5161 gctggtctgg ctggagaacg ccgccaaaaa cccaggcgcc ttcccccgtc tgtatgcctc
     5221 ctgcggaagg caagaccccc tgctgcccat caaccaattc ttccaacagc gcgcgcaaag
     5281 cctgggcttc cccctgacct acgtggagga agacggaatt cacgactggt tcttctggca
     5341 gagccagatt acgcactttc tgcggcactt ttacccggcg cgtccggaag ctgccgcgta
     5401 agggagcagc ccaaagaacg caaatgcgcg cctcacaggc gcgcggcgac cagcattagg
     5461 gtagaatcag gttatggacg cgactgacat tcccaattca gggaagaaaa tccgcctcac
     5521 ttcgctctcc agctgcgctg gctgagcttc gaagctgaac gcggaaacgc tggcgcaggt
     5581 gctgcgcccc ctgagcacgg tttttccaac ggcagattat cctgatttgc tggtcggttt
     5641 ggattccgcg gatgacgcgg cggtgtggaa gctggatgaa acccgcgcgc tggtgcttac
     5701 cacagacttc ttcaccccgg tagtggatga cccctacgac tacggcgcca tttctgcagc
     5761 caacagcctc tcggatgtct acgccatggg cgggaaacca ttcctggcgt tgaatattgc
     5821 cgctctgccc ccggacctgc ctgtggaagt gagcacagaa attatgcgcg gcgcagccga
     5881 aaaatcccgc gaagcgggcg tggtcatcgc cggcgggcac accatccagg ataaggaacc
     5941 caagttcggt ctggcagtgc ttggcatgct ccaaaccgat gctttgctaa aaaaaggcgg
     6001 cctgcaagcc ggagaccatt tgttcctcac caagccgctc ggcttcgggg tgtctaccac
     6061 cgcccataaa cgcgggctgc tctcagaaga cgaactcgcg gaggtcgtcg gctggatgaa
     6121 aaccctgaac cgggctgcgg gagagcttgc cgtgttgagc ggactgcact cggccacgga
     6181 tatcaccggc tttggcttgc tcgggcacct ttcggagatg accaaggcct ccggatgcgg
     6241 cgcaaccctg cgcatgagcg ccgtgccttt ggtctcctgt gcgctgaaat acgcgcaggc
     6301 ctacaccttc ccgggcggcg ccagcgataa ccggctttac tttgagcccg gggttcattt
     6361 cgccggggag attccggaac acgagcgtat gctcctattc gacccccaaa ccagcggcgg
     6421 gcttctgctc gcggtgccga aagacaaact gagcgcattc caacagaacg cgcaggccgc
     6481 cgccctgccc gtatgggaga ttggcaccgc cgagtctgag cccggcatcc gggtgctggc
     6541 ctgaaaaagc ccccgcaaac acttcgaggc agcgcgatat tgcgccgccc cccctttttt
     6601 tttctttgat ttattgacaa gctacccaga tgtgctatac tcattaaagc ttcattaaca
     6661 cgcgttaata gattctttac ctgggtgatg catggagaaa agggtcacaa gtcatgacgt
     6721 ggctgagctg gcgggagtat cccggacgac tgtttcgttc gtattcaatg acgacaaaca
     6781 gtattttgtc cggccggaga cccgccaaaa ggtcagagag gctgcccgta agctgagtta
     6841 ttatccgaat gcttccgcgc gggcattggc ttccaatcag gcaaaagcta tcggcctgat
     6901 catgacgcgc gacccccact acattgcggc ggataacttc ctcccccaaa ttatcggcgg
     6961 actgttagac gtggtcaaag agaaacaaat cagcctgctg attgagtggg tggagcccgg
     7021 cgagcaaacg cacacctacc tgagcctgac tcaggcaaag cacattgacg ggatgatttt
     7081 gctcacgccc cgctttgacg acccggggtt gaaggcactg gaacagctgg gaataccttg
     7141 cgtcattatg gggcaggtgc ccggttccaa tctggctttc gtggacattg acaattgcgc
     7201 ggcggcgcat gtggctgtca accatctgat tgagcttgga cattcgcgca ttgcctgcat
     7261 caccaacgcc aggctcagct actcctcggc cagccagcgg ctgctgggct accgccaggc
     7321 tctggtggac gccggcctgg atatggatga gaaactgatc cgggaagcag attttgactc
     7381 cgccagcgga tatacagcca tgcggagcct tttgagcagc ggcagcaatt tcacggcgct
     7441 ttttgccggc agtgacaccg ttgctctggg cgcgatggca gcgatccagg aagcagggct
     7501 tacgatcccc ggggatattt caatcgtagg ctttgatgac atccccgaag ctgcatttta
     7561 ctacccggga ttaacgtcgg tacgcgtacc ggcgaatgaa attgccaggc agagctgcta
     7621 ccttctcatg catttgatga aaggcgaagc gcccgcaagc acttctatca tgttacccac
     7681 tgaactcatc tcccgccaat ccacgtgtga gatagaaagg agccacaaac atcagcaagg
     7741 agagaaattc gtccatttca cgtagtttga aacactaatt tcaaaaggag aaacgatgag
     7801 atcgaaaaag caaatgattt gggcaagcat cgcgattttg cttgtcgcca gcctggttct
     7861 ggcagcctgc gggaagactg ctgaggcccc tgcggccgaa gcgcccgctg gtgaaaccgt
     7921 agccgcccta ccccaggggc aggaattggc caatgcctat gccgggcagt ataagggaac
     7981 agtcgtgacc atggctggcc ccttcactga ccaggatgaa gttgtattca atgactccat
     8041 caagggcttt gaagagaaaa ccggtattga cattcaatat gaaggctcca aggaatttga
     8101 agccaatatc aacattcgtc tggatggcgg cgaccgcccc gatatcgttg actacccgca
     8161 gcccggtctg ctgaaatacg ccgtggaaaa gggctatgcc atcgatatga ccaccctgat
     8221 caatcctgaa tggattaaga cgaactacag cgatgcctgg caggctctat ccaagatgac
     8281 cggccccaac ggcgaaatcg ttgccggtat ctgggcacgc gctaacggca agtccttcgt
     8341 cttctatccc aagaaagcct ttgatgccgc tggctacaaa gtgcccgaga cctgggatga
     8401 aatgatggcc ctcacacaac aaattgctga tgacggcgac accgcctggt gtatcggaat
     8461 cgaatccggc gccgcgaccg gctggaccat gaccgactgg atcgaagatg tgatgctgcg
     8521 cgttgctggc ggtgagaagt acgaccagtg ggtagctggc gagttgaagt tcgactcacc
     8581 tgaggtgagg gaagccctcg attacatcga agccatctgg ttcaacgatg cctacgttta
     8641 cggcggtcgt gctgccatcc ccaccatcaa ctttggtgac gcccccaaac ccatgttcga
     8701 agatccgccc aagtgctggt tcaaccgcca gggcaacttt gtgaccacct tcttccccga
     8761 agaagccgtc gctggtgtgg attacagctt cttctacctg cccggcatta aggaagaata
     8821 cggcaagccc gtcctgggcg ctggcgacat ctacgcggtc ttcaatgaca gaccggaagt
     8881 gcgcgcagtt atccagtact tctcaaccgg cgaatccctc aaggtttggg ttgagagcgg
     8941 cggcgctatc ctgacccaca acgacgcttc cctggactgg tacgtggatc ctgtgacccg
     9001 cggcgttgcc gaaaccatcc gcaatgcgac caccttccgc ttcgacggct cggacatgat
     9061 gcccggtgcg gtcggcgctg gcacattctg gaagtacatg accgattatg tcagcggctc
     9121 catcacccgt gacgaagccc tgcgacagat cgacgcgtcc tggccgaagt aagcagccag
     9181 gctcacctaa cctgaacctt ggagagcagg cgggctgcct gcctgctctc caaaccccaa
     9241 ttgaagcttg gacgaggagg tgcttgaatg aatatttccc aaacgaaaag ccccggcgcc
     9301 aacaatccga taacgaaaat cctctcctgg tttggaacgg cgattggaca attatttgtc
     9361 tccgttctgg taccggcggt tacgtttctg gttttatggc aggggtattt attcctacaa
     9421 cgggcggcgg cgccccaaat tgtgcaggtg ctcgtggcaa ttgtttgggg tgtcggcggc
     9481 gttgcactgc tgtacgtggt aaccaactgg ctggtcatga agctccccaa aaaaatctcc
     9541 aagaccttgc agccttttgt ttttgttgga cccgctgtag ttattatggg ctggtttctg
     9601 gctgtgccgg tggtacgctc gttggttgcc agtttccgga actatctcgg caccgaatgg
     9661 gttggcctgg aaaattacaa atttgccttc actgatccgc gcatgctgga gaccttccgc
     9721 aacaacctgc tctggatggt gttcggaacc tttttcagcg tggccttcgg cctgctgatt
     9781 gccaccctgg cagaccgttc caaagcagaa gttttgtata aatccattat cttcacgcct
     9841 tatgccttgt cctttgtagg cgcgggcgta atctggaaat ttatctatac ctataaaggc
     9901 acaggcatcg gcatccgcga aattggcctg ttaaacgccg tagtcaccgc actgggaggc
     9961 accccgcagc cgtggctgaa cctgacgccc tggaataatt ttttcctgat tgtcattatg
    10021 atatggctgc agaccggtta cgccatggtg attctctcca gcgcgataaa gggcgtgccc
    10081 gcggaactgc ttgaggcagc ccgcatcgac ggcgcgaacg aattcaagat tttcttcagc
    10141 attatcgttc cttacatcaa aggcaccctg ctgaccgtca cgaccacgat cgttattttc
    10201 agtctgaaga ccttcgatat tgtgcgcgtg atgaccggcg gaaataacgg cacaaacgtg
    10261 attgctaacg agttctacct gcaaagcttc acctacaaca acgcgggcag ggcatcagcg
    10321 atcgctatcg tcctgctgct gctggttaca ccggtaatta tcaacaatgt gcgctcgttc
    10381 aatcaacagc ggagggggtt ctaatggcta acagtctaaa atcccacaaa gcctctcaga
    10441 gtttctggac aaattttgtc ctgatcgtaa tctgcgtcct gtggatgatc ccgattctgg
    10501 gtatcctgat tacctctttc cgccccagcg atgaaatctt ccggaacggc tggtggaacg
    10561 tttttccgca taaagaggat gttgaagtta gccgcgtcgt catcccggag agtgtggatg
    10621 tggatggacc gatctcttat ggcggtaaaa ccgcaacctt ccaggaatgg cagcgcggca
    10681 tccaactgga tgacggcacc aaagccacct ggtacggcaa caaacgcacc cgcaccttaa
    10741 tcatttccga aaacaaatgg gtgggatttg ctaccaacct gacccttgag aactataaag
    10801 acgtgctcac cggcaagacg attacctata aggacgccca agggcagacc atcacccgca
    10861 caggcaacaa cctgggcggc gccttcctga actccctggc ggtcaccatt ccgggaacaa
    10921 tcatcccgat tctgattgcg gcttttgccg cgtttggttt cgcctggtta gacttccctg
    10981 gcaggaacgt cgtcttcacg attattgtcg ccttattggt tgttccgctg cagattgccc
    11041 tggtacccat cctgaaagac tacgtcaagc tggaactgaa cggaacattc ctcggcatct
    11101 ggctggcgca taccggcttt ggcttaccgc tggctactta cctgctattc aattacatca
    11161 gcacgattcc cagggagctg ctggaatccg cctacattga cggggcttca aacttcacca
    11221 tctttaccaa cctgattctg cccctctctg taccggcgct ggcatctttt gccattttcc
    11281 agttcctttg gctgtggaac gactacctgg tcgcgctggt gttccttggg gaaaagaacc
    11341 agaccgtgac catagcggtc gcgcaattgg tcggtgaaaa aggtcaggac tggcacctga
    11401 tgacggcggc ggccttcatc agcatgatac tgccgttagt ggtcttcctc agcctgcagc
    11461 gctacttcgt gcgcgggctg atggctggct ctgtcaaggg ctaaaaacga ttttcgatgc
    11521 gtctattgct gcgagctgcg cagcaataga cgcttgtgtg acatagcaag acccttcggc
    11581 tgcgcttagg gtgacaagag gttgtcattc tgaacgcagt gaagaatcta ctgtctctgc
    11641 ttacaaagat acttagaacg caatctcaac gacatggaaa aaaccgccca caaggatcat
    11701 gaaatgactg agaatgccct ctggtacaag aatgacattt tttacgaact gtatgtgcgc
    11761 gccttccggg acagcaactg cgacggctgg ggagacttga aaggcgtcgc tgaaaagctg
    11821 gactacctga gctatctcgg tgtggacacc atctggttgc tgccgatttc gccttcgccg
    11881 ctgcgcgacg acggctacga cgtgagcgat tactgcggca tccaccccat gtatggcaac
    11941 atgcaggact ttcaggacct ggtcgtccag gcgcatcagc gctcactgaa aatcctggtc
    12001 gaattagtgc caaaccatac ctccgaccaa catccctggt tccaggcctc gcgcgacccc
    12061 atgcatccgg agcacgaaaa ataccgcgat tattatgtct ggagcgatac ggaccaaaag
    12121 tatcaggacg cgcgcatcat cttcgtggac agcgaaaagt ccaactggac ctacgacacc
    12181 ttgcgcaagc agtatttctg gcaccgcttt ttttctcacc agccggacct gaactacgac
    12241 aacccggcgg tacaggaagc catgctgcag accgtaaagt tctggattga ccagggagca
    12301 gacggcatcc gcgtggacgc cccgccctac ttgttcgaac gcgaaggcac caactgcgaa
    12361 aatctgcccg aaacgcacct cttcctcaag cgtctgcgcg ctttcgtgga ccaatacaaa
    12421 cccggcaccc tgctcctttc cgaagccaac cagtggccgg aggatgtcat ccattatttc
    12481 ggcagcggcg atgaaatgca catgaacttc catttcccac tgatgccgcg cttgtatatg
    12541 gcgcttgccc gcggcgaccg cagcccgatt gaggaaatcc tggcgcgcac gccggagctg
    12601 ccccccagct gccagtgggg cacctttctg cgctgccatg acgaactgac ccttgaaatg
    12661 gtcaccccgg aagaacgcca atggatgtgg gacttttacg cgccagacaa gcgcatgcgt
    12721 ctgaatatgg gcatccgccg gaggctggcg cccctgctgg gtaacgataa acgcaaaatc
    12781 ctgctcatgt actccctgct gctgaccatg ggtggttcgc ccttcctgta ttacggcgac
    12841 gaaatcggca tgggcgataa catctggttg gaggaccgca acggcgtccg caccccgatg
    12901 cagtgggagg acagcccaaa cggcggcttc tcggaggcgc ccgcggaaaa attttacgcg
    12961 ccggtcatct cagaccccga atacggctgc gctcgggtga atgccgcggc ccagatggcg
    13021 gacccggatt ccctgctgaa ccacatccgg cacctcatcg cggtgcgcaa acagcatgct
    13081 ttgtttgggt ggggcgattt cgcctggcgg gaattctctt acaacccgaa gacggtagcc
    13141 gcgtaccagc gcaaattcga agggcaacgc gtggtcgtgt tgaacaacct tacggatgca
    13201 gaagtgcacg gctggatccc ggagaccgcc gccgcgttcg aggatttgct gaccggagaa
    13261 caggttctcc cgggagatta cttcctgccg cctttcggct tcgtgtggct taagcccatc
    13321 ggtccggacc tgccgaatca gccgcttgga cccaacgccc cccgaccgca gtatcttggt
    13381 tcggataagg aataatccgg gctgctcgta cccactcggc agaaaaatcc gttgaggaag
    13441 gctccgaagc agctcatctg tcaatttgca ggaattcacc agacagacag gcaaaaagcc
    13501 tgctgtttag cagaggtaac ccgatgaaaa ctcccatcta tggcggcatt gaaggcggcg
    13561 gcaccaagtt tgtgtgcgca gtaggcagcg cggacgggga aagcctggca gaaacgcgct
    13621 tcccgaccac caccccggag gaaacgctca gccgcgcgat tgtttttttc caggaacaaa
    13681 cccgcgcgct tggcccactc gcaggattag gtatagcctg ttttggcccg ttggaccctg
    13741 atatccactc ccccacttat ggacgcatcc tggccacccc caaagccggc tgggctggcg
    13801 cggatatcgt cggggcgctg ctcccagcgc tggcggttcc cattgccttt gataccgacg
    13861 tgaacggcgc ggctctgggc gaatggcgct ggggcgcggc gcagggtctt tccacttttt
    13921 tatacttcac ggttgggacg ggcgtgggcg gcggcgcgat ggtggagggc aggctcctgc
    13981 acggcttaat ccacccggaa atgggccaca tcccgctccc gcataatctc gcgcaagacc
    14041 cgttccccgg cggctgcccc tttcacgggg actgctttga agggctggcc agcggccccg
    14101 ccatggaaaa gcgctggggt cagcgcgcgg aaagcctgcc gcccgagcat cccgcctggg
    14161 acctggaggc acattacatc gcccgggcga tgcagggctt tatctgcagc ttttcaccgc
    14221 agcgcatcat tctgggcggc ggcgtccccc agcagcccgc gctccttccg ctggttcgcg
    14281 ccaaaacgtt ggagtatctg aaccattacg tcaactccga agctattctc agccatatag
    14341 acagctacat tgttcccccg ggcttgggcg cgcgcgcggg ggtgtgcggg gccttcgcgc
    14401 tggcacagca agccgctgcc ggggtgggac tatgattcac gggga
//
LOCUS       DAOQAD010000040        14246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_4644_length_14246_cov_6.200409, whole genome shotgun sequence.
ACCESSION   DAOQAD010000040 DAOQAD010000000
VERSION     DAOQAD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14246
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4644_length_14246_cov_6.200409"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..617)
                     /locus_tag="PKL60_04715"
     CDS             complement(369..617)
                     /locus_tag="PKL60_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNW95473.1"
                     /translation="MDSGMIGKIEKAKRYAEEKDRITINNFQATFKGDNNDHVVQYEQ
                     GKWTCDCSFFQTRGVCVHTMALEMVLKGMVANAEPLQS"
     gene            complement(852..1112)
                     /locus_tag="PKL60_04720"
     CDS             complement(852..1112)
                     /locus_tag="PKL60_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95474.1"
                     /translation="MRLPLEHSAEVPEGRSLMALPPCKMMVFQGEPSDDCQYEEAIGE
                     LWKRIDRFAPPRLPPCARIRAALSTGADGLLPLHQSPAGESR"
     gene            complement(1286..1783)
                     /locus_tag="PKL60_04725"
     CDS             complement(1286..1783)
                     /locus_tag="PKL60_04725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW95475.1"
                     /translation="MSSQPLPGGSGANAEPLLLPAALRDLAAERRLEKACFPREDVWP
                     LVESLWVLIKPGLVRLKLVSSGEMIGFLSGYQQVQGQIGWISSIGIAPAWQGRGLGAR
                     LLSAGERALGTPLVRLTVRASNARAIRLYGRAGYRQIQTARGYYVGGEDGLIFEKETG
                     RVQAL"
     gene            complement(1780..3711)
                     /locus_tag="PKL60_04730"
     CDS             complement(1780..3711)
                     /locus_tag="PKL60_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNW95476.1"
                     /translation="MSVLTAQNLSKAFGPVDIFEDLTFAVPRRARIAIVGPNGVGKTT
                     LLRILAGAEEPTRGSVHRARGLTLGYLPQEAVTIREGTLWSYCLMAFESLLAQADELK
                     ALERDMQDPVCPPEVLQRYGVVLSRFEHGGGYTYANRIQQTLAGLGFAESDYQRPLTQ
                     LSGGQRTRAVLARLLLDEPDLLLLDEPSNHLDIAAVEWLEAWLRDWNGAVIIVSHDRY
                     FLDQTASQVWEMTPALEVYRGNYTAYLTQREARYTRRLAEFEAQNEYIEKQEDYIRRF
                     LGTQNNNQARGRQRRLERLLADSRLSAPKANIRPMHLRLSLAGRSGDLVLRSYSLQVG
                     YHDEGRPLFHAPDLVLKRGECVAVIGPNGAGKTTFLKTILGQIPPYAGRIELGASLKV
                     GYFAQAHEGLNPGLTLIEEINSVAPSMLPAEARNYLAKYGFTGEEVFARVQTLSGGER
                     GRLALAKLALTDANLLLLDEPTNHLDLPTQEVLQNVLADYPGTILLVSHDRYLIDALA
                     SQIWEMEPAASRLKVFSGTYSEYKIAQALSQQPAPEQAPTAPEPLPEPAAADAPARKA
                     LSRNQRQQIQKKLDALEARIRALESESALQEAQLANPPADPRKTALLAESYNKLRAEL
                     EPLLSEWEALATTLQEDAP"
     gene            4519..5601
                     /locus_tag="PKL60_04735"
     CDS             4519..5601
                     /locus_tag="PKL60_04735"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNW95477.1"
                     /translation="MKLKRWHIWLGVAISAVFLYLAFRKVDFSLVWQYLKQAQWSWVL
                     LGLAVYFFGVLLRAWRWQILLNPLKRLPIRRLFPVVAIGYMGNNVYPARAGEVLRSVL
                     LKSKDQVDISGSLATIVVERLFDAIVILGLVLLNLRQLSAMPAASEWVGSIQKGAIIG
                     GIVFLLLLLVFLAMVFFPVQTQKLSAWVIHRIIPSRFRASLNGIVARFITGLSSLRSP
                     LETLKVLLLSIAIWTCETGLYWGVMRALGLDLSFMTLMLLNGVVNLVLLVPAAPGGLG
                     TFDAACKAMLVLFGIGSELALGYTLLLRAALWLPITVLGALFFLREGFTLSTDVGKLK
                     EKYSGQETSGAPAEVLADSQEETEKS"
     gene            5598..6932
                     /locus_tag="PKL60_04740"
     CDS             5598..6932
                     /locus_tag="PKL60_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNW95478.1"
                     /translation="MTELSRKPNIAVIGAGIGGMSAAYDLVRAGKSVTLFEASDRPGG
                     LAAGFKEPHWDWSVERYYHHWFTSDKHMLALIEELGWSDQVIFPKPVSVMYYQGKFYP
                     FDSISAALLYPGLGWGINKIRFGLVGLYLRLTKSWQTLEKTTTDAWMRAKAGDKVYES
                     MWEPMMIGKFGERYAKVVNMAWMWARLHSRSTRLGTFIGGFQTFADKFAARLEELGVG
                     IVYETSVESIEQAQDGKVLLRFSDREPQEYDQALVTLSPGIMARLTPGLPQDYLAGLL
                     NLKSMGAVVMTLSLKHALSPQGYYWYNIPKQVGFPFLSLVEHTNFLKPEYFGGDHILY
                     IGDYLEVDHPNFRKSEEELLAEVLPHLTRINPEFQPDWVKKVWVSKTPYAQPIPLVNH
                     SKNIPPIKTPVPGVFFASMSQVYPWDRGTNFAVEIGRRAAKMMLAEDGPSGA"
     gene            7391..8722
                     /gene="dnaA"
                     /locus_tag="PKL60_04745"
     CDS             7391..8722
                     /gene="dnaA"
                     /locus_tag="PKL60_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNW95479.1"
                     /translation="MNAQQAWQAAQGQLQMELSKATYDTWVKQTQLIRFDEPAGVFYL
                     GATNAYACDWLESRLKATLINKLSGMMARPVEICFSVWAAQSVEQTLEEVVIPAREEP
                     VRDMFETHLAPKYRFDNFVVGPNNRLAHAASLAVAEHPARAYNPLFLYGGVGLGKTHI
                     LHAIGNSVLQQKLQVLYVSSEEFTNDLVNAIRTKTTAAFREKYRQVDVLLIDDIQFIS
                     GKESTQEEFFHTFNTLHGQDKQIVMTSDRAPKAMSTLEERLRSRFEWGLTVDIQPPDY
                     ETRLAILRTKAEKANREVPTEVMEMIAREVQANIRELEGAWNRVLAYADLSGEKLTLQ
                     LANNAMVDILPQRSALVPNEVISTVSKFFGISTERILSKDRSKDVALPRQVAMYLMRE
                     LGGVSLPKIGEELGGRDHTTVMYACDKVADMIESDDRIRRQVLQLREQLIG"
     gene            complement(9021..11837)
                     /gene="polA"
                     /locus_tag="PKL60_04750"
     CDS             complement(9021..11837)
                     /gene="polA"
                     /locus_tag="PKL60_04750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNW95480.1"
                     /translation="MPKILYLLDGHALAYRAYFALTAGGSTRWATSTGEPTAGVFGFA
                     STLLRILEQEQPDYLAVSFDTGRTFRHDLYPEYKGTRAKMPEDLRGQVERIREMVDAF
                     NIPRLEVENYEADDVIGSLAVWASQEEGLGVKIITGDRDLLQLVTNRVVVSLPNNKTN
                     TNEDYFPEDVQRKLGVLPEQVVDYKALVGDASDNIPGVKGIGEKTAVSLLEKYGSLDN
                     IYAHLNEISGRAGAALAAGKPEAYLSQDLARIRTDLKLRFDLTQAESDRFNPGAVEDL
                     FRQLEFRTLTQRLHNLVRKLHPSLERAAQQSLFAEPPTPAVELFRSGDYTTEVVDTPG
                     KLQAAAAELAKASRLAFDTETTSTDPMRAELVGISLAADPSIGYYFPLGHKQGRQLAR
                     EEVFQALQPLLENPGISKVGHNSKYDLICLEQAGLRVAPIDFDTMLAEWLINPDSRNL
                     GLKNLAWVRLGAEMTHIEELIGRGKNRISMAEVLIEEAAPYAAADAVMTLRLVEPLQA
                     ELTSHQVERLNRDLEVKLIPVLADMEQKGILLDAGMFESFAQELNGQIQRIEESIYAR
                     VGEPFNINSTQQLSDVLFKRLGLEPPDRSRKTSAGKLSTAVDVLEGMRGQHPIIGEIL
                     EYRELAKLKSTYVDALPLQVNPKTGRIHTSFNQTGTVTGRIASQDPNLQNIPTRTELG
                     RRVRQGFIAAPGAKLLSIDYSQIELRIMAHISQDEAMLEAFRQGQDIHAATAAAIYNI
                     PLEKVDKNQRRHAKAINFGLIYGMSAFGLTRATELTLAEAENYIKAYFEHFPRVKTWL
                     DNTRVLAAKQGYVETLLGRRRYFPNLQTGTNYAMRQREEREAINAPIQGTAADIIKLA
                     MIALPDAFAKARLHGCMLLQVHDELIFEVPEKELAETIATARSVMEHTMRLDVPLLTE
                     ARTGSTWGELEPVSDSVIEDLTGA"
     gene            complement(11891..12412)
                     /locus_tag="PKL60_04755"
     CDS             complement(11891..12412)
                     /locus_tag="PKL60_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95481.1"
                     /translation="MPQDLEPLLASFLPASSAAAVFISANHQVSSSVQPALRGFSAQI
                     IRYIETLQPPAAGSAGEYHLMDLGGQKLVLVSSLLNPSQPVSLLFPTETSFDVILNAF
                     SALEKLLNAPELPDLHDLKARKNSSAHPQRWQDALFDPAPQSPAEAGLPTRAENKPNA
                     APLPVSDGWTLIL"
     gene            12572..13969
                     /gene="lpdA"
                     /locus_tag="PKL60_04760"
     CDS             12572..13969
                     /gene="lpdA"
                     /locus_tag="PKL60_04760"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNW95482.1"
                     /translation="MENFDVVVVGAGPGGYVSAVRAAQLGFRTALVEQKALGGVCLNI
                     GCIPSKALLRNAELAYLLRTNADSYGIRMDNLSLDYGAAFKRSRQVSQRLTKGVGFLM
                     KKHGISVFNGRGCLLNASEVRVDAGDGSSQTLGAQHIILATGAHPAVVPGMQPDGEKI
                     LDYAHAIMLEQLPKSALIIGGGAVGVEFATIWSAYGVEVHLVEMLPHILPAEDDEAAA
                     ELTKAMLKRGVKVLAGTKVLGVEKTEGGTRVSLANEAGEQSVETDLTLVAVGFKPNSR
                     DLGLEALGVELDQRGFVVVDGQMRTNIPGVRAIGDLTGKLLLAHSASAMGILCAESIA
                     GLPARELDYRMIPRATYSQPQVASFGYTEAQAEQAGFRVRVGKFPFQANGKALGYGEY
                     GGYVKLIANAESGLLLGASIVGPDAAELLPELTLAQANGLPVRAIAANIHAHPTLSEA
                     VLEAAEVVEGRAIHS"
BASE COUNT         2759 a         4087 c         4412 g         2988 t
ORIGIN      
        1 gttggaagcg ccctgcctgg acgcccttgt acaggtatgc acacccggga aaggaatagg
       61 acagcgagcc tttcacctcg cgggctctgt atgagtgaat aaatttgaac gataaagaaa
      121 gcccgcggtt cttgttccgg gaacgcttta gaatacaaaa aacgctggca accgactctg
      181 ccggttgatt ccagcggact tgagagtaaa tttatctttc cgcatgacgc taatatgcgg
      241 cacaggttta agtagagaca gacaccggtt actcaggtgt ctgtcctctc atcttatagg
      301 gaaattgtcg aattttctaa tagccagggg gcggctcagc caaaacgcgc ggacggcctg
      361 tgaaggtttc agctctgcaa tggctcagcg ttcgcgacca tgcctttcag caccatctcg
      421 agcgccatgg tatgcacgca aaccccgcgg gtctggaaga acgagcagtc gcaggtccat
      481 ttgccctgct cgtattgcac gacatggtcg ttattatccc ctttgaatgt ggcttggaag
      541 ttgtttatcg tgatgcgatc tttctcttcg gcgtatcgtt tggctttttc gatttttccg
      601 atcattccgc tatccatgct gtcctaagct ccgtttctct ggaatattta aacaaaaaca
      661 cgcgccaaaa acaaaaggcg tgtgttccgc tgcaagcatc caacgcagta cctccgcgcg
      721 gatcatttta tttaagcgaa ctccttcatg atgctttcat tatactccaa attaagagat
      781 atttctgagg tttcagcatc ttttttcgta ccagccggaa tttagaccgg ctggcgctgc
      841 ctgttctcgc gttaacggct ctcaccggca gggctttggt gcagcggcag cagcccatcg
      901 gcgccagtgg aaagcgcggc gcggattcgg gcacacggcg gcagccttgg gggggcgaag
      961 cggtcgatac gcttccagag ttcaccgatg gcttcttcat actggcagtc gtcggagggc
     1021 tccccctgga agaccatcat cttgcacggc ggcagggcca tcaggctgcg gccctccgga
     1081 acttccgcgc tgtgctccag gggcagtctc acgccttgca cgcaccggca ggcggcaagc
     1141 ccggtggggt ccggggaggt gcgaacagag gcaaatgatc gtgcaggccg ggcgctttgc
     1201 aggaatgcga atttccggcg agcgcaccaa ttttgggtgg atctgtttga tggaagcgct
     1261 ttgggagctt tgggagaagc gggttttaga gcgcctgaac gcgcccggtt tctttttcga
     1321 aaatcaggcc gtcctcgccg cccacgtaat atccacgcgc ggtttggatt tggcggtagc
     1381 cagcccgccc gtacaggcgg atggcgcgcg cgttggatgc gcgcacggtc aggcgcacaa
     1441 gcggcgtgcc aagcgcgcgt tcgccggcag agagcaggcg cgcccccagg ccccgcccct
     1501 gccaggcagg cgcaatcccg atggaagaaa tccaaccgat ttggccctga acctgctgat
     1561 atccggagag aaagccgatc atctcgcccg agcttaccag cttcaggcgc accaagccgg
     1621 gcttgatgag cacccacagc gattccacca gcggccagac gtcttcgcgc gggaagcaag
     1681 ctttctccag gcggcgctcc gccgccaggt cgcgcaaggc tgccggcagg agtaacggtt
     1741 cagcgttcgc tccactgccg ccaggcaggg gctggctgct cacggcgcgt cctcctgcag
     1801 ggtggtagcc agcgcttccc attccgaaag gagcggctcc agctccgccc gcagcttgtt
     1861 gtaactctcc gccagcagcg ccgtctttcg ggggtcggcg ggagggttgg caagctgcgc
     1921 ctcttgcagg gcgctctcgc tctccagggc gcggatgcgg gcttccagcg cgtccagctt
     1981 cttttgaatc tgctggcgct ggttgcggct gagcgctttg cgcgcggggg cgtcggctgc
     2041 cgctggctcc gggagtggct ctggggcggt aggggcttgc tccggcgcgg gctgctgaga
     2101 gagagcctgc gcgattttat attccgaata ggtgccgcta aaaaccttga gcctgctggc
     2161 agccggttcc atctcccaga tttggctggc aagcgcgtca atcaggtaac ggtcgtgcga
     2221 gaccagcaga atggtgccgg ggtaatccgc cagcacgttt tgcagcactt cctgggtagg
     2281 caggtccagg tggttggtgg gctcgtccaa cagcagcagg ttggcgtctg tcagcgccag
     2341 tttggccagc gccaggcgtc cgcgttcacc gccggagagg gtttggacgc gcgcgaagac
     2401 ctcctcaccg gtgaagccgt acttcgccag atagttgcgc gcttctgccg gcagcatgga
     2461 cggggcgacg ctgttgattt cttctatcag ggtcagcccg gggttcagcc cctcatgcgc
     2521 ctgggcgaag tacccgactt tcaggctggc gccgagttca atccgcccgg cgtagggagg
     2581 gatttgaccg aggatggtct tcaggaaggt ggtcttacct gcgccgtttg gtccgatgac
     2641 agccacgcac tccccgcgct tgagcaccag gtccggcgcg tgaaaaagcg ggcgaccttc
     2701 gtcgtgatag ccgacctgca ggctgtagct gcgcagcacc aggtcgccgg agcggccggc
     2761 cagcgagagc ctgaggtgca tcgggcggat attggccttg ggagcgctca ggcgtgagtc
     2821 cgccagcaag cgttccaggc ggcgctgcct gccgcgcgcc tgattgttgt tctgcgtgcc
     2881 taaaaagcgg cggatgtagt cctcctgctt ttcgatgtac tcgttttggg cttcaaattc
     2941 tgccaatcgg cgggtgtagc gcgcttcccg ctgggtcagg taggcggtat agttgccgcg
     3001 gtagacctcc agggcaggcg tcatctccca gacctgcgaa gcggtctggt ccagaaagta
     3061 gcggtcgtgc gagacaatga ttaccgcgcc gttccaatcg cgcagccagg cttccagcca
     3121 ttccaccgcg gcaatatcca ggtgattgct gggctcatcc agcagcagca ggtcgggctc
     3181 gtccaaaagc aggcgcgcaa gcacggcgcg ggtgcgctgg ccgccggaaa gctgggtgag
     3241 cgggcgctga tagtccgatt cggcaaagcc cagccccgcc agcgtctgtt ggatgcggtt
     3301 ggcgtaggtg tagccgccgc cgtgctcaaa gcgggaaagc accaccccgt agcgctgcag
     3361 cacttcaggc gggcaaaccg ggtcttgcat atcgcgctcg agcgctttca gctcatccgc
     3421 ctgggcaagc aggctctcga aggccatcag gcagtaggac cagagcgtgc cctcgcggat
     3481 ggtaacggct tcctgaggca ggtagccgag tgtcagcccg cgggcgcggt gcaccgagcc
     3541 gcgcgtgggt tcctcggcgc cggccaggat gcgcagcagg gtggttttac ccacgccgtt
     3601 gggcccgacg atggcgatgc gcgcgcgccg ggggacggcg aaggtcaggt cctcaaagat
     3661 atcgaccgga ccgaaggcct tgctgaggtt ttgggcggtg agaacggaca tagcgtttga
     3721 agtataccag cgatggaagg ccggggcggg ctttacgggg ctgcttgcgt gctgcaggct
     3781 aagccggcga gcgcaggaag actgatgagg gtttgtttcg cccctccgac ctgatagaag
     3841 aggggacctg cggtcaaccc cacgcgctgg tgaggacacc agcgcgatca agaggaaaag
     3901 gttgtcgtcg cgaggagcgc agcgacgtgg cgatccgcct ttcattttct actttcctga
     3961 gcaaatcaca gtaaactgca gattgctgcg cctcccccag tgatatccag aattatgtca
     4021 agtttccagc gggggttcgc aatgacgaat ctctacgaac tgataaccgc gagacctgcg
     4081 gtcaacccca cgcgctggtg aggacaccag cgcgatcaag gaaaaggttg tcgtcgcgag
     4141 gagcgcagcg acgtggcgat ccgcctttca ttttctactt tcctgagcaa atcacagtaa
     4201 actgcagatt gctgcgcctc ccccagtgat atccagaatt atgtcaagtt tccagcgggg
     4261 gttcgcaatg acgaatctct acgaactgat aaccgcgaga cctgcggtca accccacgcg
     4321 ctggtgagga caccagcgcg atcaagtccg cagcctgctc catgacaata cattgggtgg
     4381 gcaaagcctc ccataacata ctcccgctcg acaaaacatt gcctctcagg atgcttcgcc
     4441 cctacctctg ccgctgaatt cacgctcagc ctgacaccag tcttctccat ccgccgctct
     4501 caatggtaaa atcggggcat gaaactaaaa cgttggcata tctggcttgg cgtggcaatc
     4561 agcgcggtct ttctgtatct ggctttccgg aaagtggact tttccctggt ctggcagtat
     4621 ctcaaacagg cgcagtggtc ctgggtgctg ctggggctgg ccgtctactt tttcggcgtc
     4681 ctgctgcgcg cctggcgctg gcaaatcctg ctcaacccgc tcaagcgcct gcccatccgc
     4741 cggctcttcc cggtggtggc catcggctac atgggcaaca atgtctatcc tgcccgcgcc
     4801 ggcgaggtgc tgcgttcggt cctgctcaag tccaaggatc aagtggatat ctccggcagc
     4861 ctggcgacca tcgtggttga acgcctgttc gacgcaatcg tgattctggg cctggtgctg
     4921 ctgaacttgc gtcaactgag cgccatgccg gcggcgtcgg agtgggtcgg aagcatccag
     4981 aaaggcgcca tcattggcgg catcgttttt ttgctcctgc tgcttgtttt ccttgccatg
     5041 gtgttcttcc cggtccagac ccaaaagctc agcgcctggg tgattcaccg aattatcccc
     5101 tcccgtttcc gcgcgtctct gaatggaatt gtggcgcgtt tcatcaccgg gctttccagc
     5161 ctgcgctccc ccctggagac cctgaaggtg ctgctgctct ctatcgccat ctggacttgt
     5221 gaaaccggtc tgtattgggg cgtgatgcgc gcgctggggc tggacctgag ctttatgacg
     5281 ctgatgctgt tgaacggggt ggtgaacctc gtcctgctgg tgccggcggc gccgggcgga
     5341 ctgggcactt ttgatgcggc ctgcaaagcc atgctggttt tgttcggcat cggttccgag
     5401 ctggctctgg gttatacctt gctgctgcgg gcggctcttt ggctgcctat cactgtgtta
     5461 ggcgcgcttt tcttcctgcg ggaaggtttc acgctgagca ccgacgtggg caaactaaag
     5521 gaaaaatact caggtcagga aacatcaggg gcgcccgctg aagttttggc ggattcccag
     5581 gaggaaacgg aaaaatcatg acagagcttt cacgcaaacc aaacatcgcg gtaattggcg
     5641 ctggaatcgg cggtatgagc gctgcttacg acctggtgcg ggctggcaaa tctgtgaccc
     5701 tgtttgaggc ttccgaccgc ccgggcggtt tggcagccgg cttcaaggag ccgcattggg
     5761 actggtcggt ggagcgctac taccaccact ggttcacctc cgataaacac atgctcgccc
     5821 tgatcgagga actgggttgg tctgaccagg tcattttccc caagcctgtc agcgtgatgt
     5881 attaccaggg caagttttac cccttcgatt ccatcagcgc cgccctgctt tatccaggcc
     5941 tgggctgggg catcaataag attcgcttcg ggctggtggg cctgtacctg cgcttgacga
     6001 aaagctggca aacgctggaa aaaaccacca ccgatgcctg gatgcgcgcc aaagccgggg
     6061 ataaggtgta cgagagcatg tgggagccga tgatgattgg caaatttggc gagcgctacg
     6121 ccaaagtagt aaatatggcc tggatgtggg cgcgcctgca ctcccgcagt acgcgcctgg
     6181 gcaccttcat cgggggtttt cagactttcg cggataaatt cgcggcgcgc ctggaggagc
     6241 ttggggttgg cattgtgtat gaaaccagcg ttgagagcat tgaacaggct caggatggca
     6301 aggtcttgct gcgcttttcg gaccgtgagc cgcaggaata tgaccaggcg ctggtcacgc
     6361 tttcgccggg catcatggcc cggctcaccc ccgggctgcc gcaggactac ctggccggct
     6421 tgctgaacct gaagagcatg ggcgcggtgg tgatgacgct ttccttaaaa cacgcgcttt
     6481 ccccgcaggg atactattgg tacaacatcc ccaagcaggt gggctttccg ttcctgtccc
     6541 tggtggagca caccaacttt cttaagccgg aatattttgg cggcgaccat atcctgtata
     6601 tcggcgacta tctggaggtg gaccacccga acttccggaa gagcgaggag gaactgctgg
     6661 cggaggtgct gccgcatctg acccgcatca acccggaatt ccagcccgat tgggtgaaaa
     6721 aggtgtgggt gtccaagact ccttacgctc agcccatccc gctggtgaac cactccaaaa
     6781 acatcccacc catcaagacc cctgtcccgg gcgtgttctt cgcaagcatg agtcaggtat
     6841 acccatggga ccgcgggact aattttgcgg tggaaatcgg gcggcgggcg gcaaagatga
     6901 tgctcgctga agatggtcca tcgggcgcat agccgaatgc cctcccgaat gctgattcac
     6961 aatcgggaat cactatccgg aatcccagtg gtctgtattt ccgggatggg gtgggattaa
     7021 ttatcctaat taaagatatt aaatctggaa acgcggcata aacccctata tatagggata
     7081 gtagtgatgg cctctcctcg catttagggg cgtgtttttc ctgctgcagg ccggcgaaat
     7141 attttgaaga ctctgataaa ttttaagaat agggaatctt aaagatttgc caagttttaa
     7201 gccggagcgg gcactaactt cttgaagcca gggggagggg gtgttagaat cggcataccg
     7261 aactgaagaa caactgaaga caagttcttt cagcggatcc acatcatcgg gatacacgtg
     7321 attcgcttat gaaactgttg ttccggtcag gaattttccc cgaacgccgc ggggcatcag
     7381 gagatttgcc atgaacgcac agcaggcctg gcaggcagcg caaggacaat tgcagatgga
     7441 gctttccaaa gcgacctatg atacctgggt gaaacagacc cagcttatcc gcttcgacga
     7501 gcctgccggg gtcttttatc tgggcgccac caatgcctat gcctgcgact ggctggaaag
     7561 ccgcctgaaa gcgaccctga ttaacaagct gagcggaatg atggcgcgcc cggtggagat
     7621 ttgtttttcc gtctgggcgg cacagtccgt ggaacaaacc cttgaagagg tggtgattcc
     7681 ggcgcgggaa gaacccgtcc gggatatgtt cgaaacccat ctggctccca aataccgctt
     7741 cgacaacttt gtggtcggcc ccaacaaccg cctggcgcat gccgccagcc tggctgtagc
     7801 cgagcacccc gcccgcgcct acaatccgct cttcctctac ggcggggtgg ggctgggcaa
     7861 aacgcacatc ctgcacgcca tcggcaactc cgtgctgcag cagaaactgc aggtgctgta
     7921 tgtgtcctcc gaggagttca cgaacgacct ggtcaatgcc atccgcacca aaacgacggc
     7981 cgctttccgc gagaagtacc gtcaggtgga tgtgctgctc attgatgata tccaattcat
     8041 ctctggtaag gaatccacgc aggaagagtt tttccacacc ttcaacaccc tgcacgggca
     8101 ggataaacaa atcgtaatga cctctgaccg ggctcccaag gcgatgagca ccctggaaga
     8161 acggctgcgc tcgcgctttg agtgggggct gacggtggac atccaaccgc cggattatga
     8221 aacccgcctg gcaatcttgc gcacgaaagc cgaaaaagcc aaccgggaag ttcccacaga
     8281 agtcatggaa atgattgcga gagaagtgca ggcgaatatc cgcgagttgg aaggcgcctg
     8341 gaaccgcgtg ttggcttatg ccgacctgag cggcgagaag ctgaccctgc agctggcgaa
     8401 caacgccatg gtggatattt taccgcagcg cagcgcgctg gtgcccaacg aggtcattag
     8461 cactgtctcg aagtttttcg gcatttccac cgaacgcatc ctgagcaaag accgctccaa
     8521 agacgtggcg ctgccgcgtc aggtggcgat gtatttgatg cgcgagcttg gcggggtttc
     8581 gcttccgaag atcggcgagg agttgggcgg tcgggaccac accaccgtca tgtatgcctg
     8641 cgacaaagtg gcggatatga tcgagtccga tgaccgcatc cgccggcagg tgctgcagct
     8701 gcgcgagcag ttgatcgggt aagctggtcc cccgtcatcg cgagcgcagc gcggcgatct
     8761 gtccctcgca cagagggagg ttgcttttgt cccttgcgca ggcaagcagt cgcaatgatg
     8821 gattaaaagg cagcttgaat agcgttaact acccaacatt tcgtcatcgc aaggcacgaa
     8881 gcgatctccc gaccaaccta aaaggcagat tgccgccccc ctccagtaat tttcaggttc
     8941 ggatcaggtt tccagcgggg gctcgctaat gacgaatctt gcagggggct tgaaagacac
     9001 aagccgccct acaactactt ttacgcgcct gtcaaatcct cgatcaccga atcgctgact
     9061 ggctccagct cgccccaggt gctgcctgtg cgtgcctccg tcagcagcgg gacgtccagc
     9121 cgcatggtgt gttccatcac gcttctggcg gtcgcaatgg tttccgccaa ttccttttcc
     9181 ggcacctcaa aaatcagctc atcgtgcact tgcagcagca tgcagccgtg taaacgtgcc
     9241 ttcgcgaaag catccggcag cgcgatcatc gccagcttga tgatatcggc tgccgtaccc
     9301 tggatgggcg cgttgatggc ttcccgctct tcccgctggc gcatggcgta gtttgtgccg
     9361 gtttgcaggt tggggaagta gcgccgcctg ccgagcagag tttccacgta gccctgcttg
     9421 gctgccagca cgcgcgtgtt atccagccag gttttcaccc ttgggaagtg ctcaaaatag
     9481 gctttgatgt agttttcagc ttccgccagc gtcaattcgg tcgcgcgggt caggccaaag
     9541 gcgctcatgc cgtagatgag gccgaagtta atggctttgg cgtgccggcg ctggttttta
     9601 tccacctttt ccagcggaat gttgtaaatc gcggcagcgg tggcggcgtg aatgtcctgc
     9661 ccctggcgga acgcctccag catcgcctcg tcctgggaaa tgtgcgccat gatgcgcagc
     9721 tcaatctgcg agtagtcgat cgagagcagt ttggcgcccg gcgcggcgat gaaaccctgc
     9781 cgcacgcgcc ggccgagctc cgtgcgggtg gggatgtttt gcaggttcgg gtcttgcgag
     9841 gcaatccgcc ccgtgacggt gccggtctga ttaaacgaag tgtgaatccg gccggttttc
     9901 gggttaacct gcagcggaag ggcgtccacg taggtggatt tcagtttggc caattcgcgg
     9961 tattccagga tttcgccaat aatcgggtgc tgtccgcgca ttccctccag cacatcgacc
    10021 gcggtggaaa gcttgcccgc actggttttg cgcgagcggt caggcggttc cagccccagg
    10081 cgcttaaaga gcacgtccga aagctgctgg gtggagttga tattgaaagg ctcccccacc
    10141 cgcgcgtaga tggactcctc aatgcgctgg atttgtccgt tcagttcctg cgcgaagctt
    10201 tcgaacatgc ccgcgtccag caggatgcct ttttgctcca tgtccgccag gaccgggata
    10261 agtttcacct ccaggtcgcg gttcagcctt tcgacctgat gggaggtcag ttcagcctgg
    10321 agcggttcca ccaggcgcag ggtcatcaca gcatccgctg ccgcgtaggg cgcggcctct
    10381 tctatcagga cctccgccat gctaatccgg ttcttccccc ttccgatgag ctcctcgatg
    10441 tgggtcattt ccgcccccag gcgcacccag gccaggtttt taagccccag gttgcgcgaa
    10501 tcagggttaa tcagccactc ggccagcatg gtatcaaaat caatcggcgc cacccgcagg
    10561 ccggcctgct ccaggcaaat caagtcgtac ttgctgttgt gcccgacctt gctgatgccc
    10621 gggttttcca gcagcggctg cagcgcctga aatacttcct cacgcgccaa ctgcctgccc
    10681 tgcttatgcc cgagcgggaa ataataccct atggaggggt ccgccgccag cgaaattcca
    10741 accaactccg cccgcattgg gtcggtgctg gtggtctcgg tatcaaaggc cagccggctg
    10801 gcctttgcca gttccgcggc ggctgcctgc agtttcccgg gggtgtccac gacctcggtg
    10861 gtatagtcgc cgctgcggaa cagttccacg gctggggtgg gcggctcggc gaacaggctc
    10921 tgctgcgcgg cgcgctccag gctgggatgc agcttgcgca ccagattgtg caggcgttgg
    10981 gtgagggtgc ggaactcgag ctgacggaag aggtcttcca ccgcgcccgg gttaaagcgg
    11041 tcggattctg cctgggtgag gtcaaagcgc agtttcaggt cggtgcggat gcgcgccagg
    11101 tcctggctga ggtaggcttc aggcttgcct gctgccagcg ctgcgcctgc ccggccgctg
    11161 atttcgttca ggtgcgcgta gatattatcc agactgccgt acttttccag caaactcacg
    11221 gcggttttct cgccgatgcc tttcacgccc ggaatattgt ccgaggcatc tccaaccagc
    11281 gctttgtagt ccaccacctg ttcaggcaaa acacccagtt tgcgctggac gtcctccggg
    11341 aaataatctt cgtttgtgtt ggttttgtta ttgggcaaag acaccaccac ccggtttgtg
    11401 accagctgaa gcaggtcgcg gtcgccggtg atgattttga cgcccaagcc ctcttcctgt
    11461 gaagcccaga ctgccaggct gccaattacg tcatccgcct cgtaattttc cacttccaac
    11521 cggggaatgt tgaacgcgtc caccatctca cggatgcgtt ccacctgacc gcgcaggtct
    11581 tcgggcatct tggcgcgcgt gcccttgtat tccgggtaga ggtcgtggcg gaaagtgcgc
    11641 ccggtatcga aagaaaccgc caggtaatcg ggctgttcct gctccaggat gcgcagcagt
    11701 gtactggcga aaccaaacac gccggcggtg ggttcacccg tactggtggc ccagcgggtg
    11761 ctgccgccag ccgtaagcgc gaagtaggcg cggtaggcta acgcgtgtcc gtccagaagg
    11821 taaaggattt tcggcatggt aacctcggtt ctgcaggttt gagcgggaag tttgcgaacc
    11881 tcccgggggt tcagaggatg agcgtccagc catcggaaac tggcagtggc gcggcgtttg
    11941 gcttgttttc cgcccgggtt ggcaagcctg cttccgcagg gctttgaggc gccgggtcaa
    12001 agagcgcgtc ctgccagcgc tgcgggtgcg cggagctgtt ttttcgcgct ttcaaatcgt
    12061 gcaggtccgg cagctccggc gcgttcagca gtttttcgag cgcggaaaag gcgttcagaa
    12121 tcacgtcgaa actggtttca gtcgggaaaa gcaggctgac cggctgggat ggattcagca
    12181 ggctgctgac cagcaccagc ttttgcccgc ccaggtccat cagatggtac tcacccgcag
    12241 agccggcggc tggtggctga agagtctcga tataacggat aatttgcgcg ctgaatccgc
    12301 gcaaagccgg ctggacggaa gatgatacct gatggttggc agagataaaa acagctgcgg
    12361 cggagctggc gggaaggaag ctggcaagaa gcggttcaag atcctgcggc atgattcatc
    12421 accccgatga cggggctctc cttatctaaa gaagtttagc atgccttctg gtcgggtgca
    12481 agcagacagg ctccaggctg tgcgcgcgga atcagactgg agtctgcctt tataattggc
    12541 ttaaccagaa aagaaaacgc taaggagcat aatggaaaac tttgatgttg ttgtggttgg
    12601 cgcggggccg ggcgggtatg tcagcgcggt tcgcgcggcg cagttgggtt tcaggaccgc
    12661 gctggtggag caaaaagcat tgggcggggt atgcctgaat attggctgca ttccttccaa
    12721 ggcgctcttg cgcaacgctg aactggcgta cctgctgcgc accaacgcag acagctacgg
    12781 catccggatg gacaacctga gcctggatta cggcgcggct tttaagcgca gccgtcaggt
    12841 ctcccagcgc ctgaccaagg gcgtgggctt tctgatgaag aaacacggca tcagcgtgtt
    12901 caacggacgc ggctgcctgc tcaacgcctc ggaagtgcgc gtggatgccg gcgacggcag
    12961 cagccaaacg cttggtgcgc agcatatcat cctggcgact ggcgcgcatc cggccgtagt
    13021 gcccgggatg caaccggacg gcgaaaaaat cctggattat gcgcatgcga ttatgctgga
    13081 gcagctgccc aaatccgcgc tcatcatcgg cggcggggcg gttggggtcg aatttgccac
    13141 tatctggagc gcgtacggcg tggaggtgca cctggtggag atgctcccgc acattctacc
    13201 cgccgaagac gacgaagcgg cggcggagct gaccaaagcc atgctaaaac gcggtgtgaa
    13261 agtgctcgcc ggcaccaaag tgcttggggt ggaaaaaact gagggcggca cgcgcgtgag
    13321 cctcgcgaac gaggctggcg agcagagcgt ggagactgac ctgaccctgg tagccgtggg
    13381 gtttaagccc aactcccggg atttgggctt ggaagcattg ggcgtggagt tggaccagcg
    13441 cggttttgtg gtggtggatg ggcaaatgcg caccaatatc cccggcgtgc gcgccatcgg
    13501 agacctgacc ggcaagctgc tgctggcaca ttccgcctcg gccatgggca tcctgtgcgc
    13561 ggaaagcata gccggactgc ccgcgcgcga actggattac cgcatgatcc cgcgcgcgac
    13621 ctacagccag ccgcaggtgg cttcatttgg gtataccgaa gcacaggctg aacaggcagg
    13681 ttttcgggtg agagtgggca agttcccctt ccaggccaac ggcaaagcgt tggggtatgg
    13741 ggagtacggc ggctatgtca agttgattgc caacgcggag agcggtttgc tgctgggcgc
    13801 gtccatcgtt ggtccggacg ccgcggagct gctgcccgaa ctgacgctgg cgcaggccaa
    13861 cgggttgccg gtcagagcga tcgcggcgaa catccatgcg cacccgaccc tgagcgaagc
    13921 agtgttggaa gcggcggagg ttgtggaagg ccgcgcgatc catagctgag cgcgcctgcc
    13981 caaaggggat tgccggcagg ggcgaagcat ccagtccaag cgcctttgct taagcagatg
    14041 cgcataccaa ggatgctttg cccgcgtcac gggattgttc tcacggatct caggaatggt
    14101 gtgatcgaag gactggagac cggcgcgatc tcaggagatt tggagacctg gcggtcagtc
    14161 tccgcgcgcg gtcaggaggc cggcgcgctc aggggctcgc aacgacgccc gattcgtcat
    14221 tagcccccgc tgtaggaatg gctcaa
//
LOCUS       DAOQAD010000041        14214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_4656_length_14214_cov_6.511759, whole genome shotgun sequence.
ACCESSION   DAOQAD010000041 DAOQAD010000000
VERSION     DAOQAD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14214
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4656_length_14214_cov_6.511759"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1276
                     /locus_tag="PKL60_04765"
     CDS             125..1276
                     /locus_tag="PKL60_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNW95483.1"
                     /translation="MKYRPFGKLDWQASALGFGCMRFPTLEGDSGLIDEDQAAPMLRR
                     AIDAGVNYIDTAWSYHREQSERFVGKVLARGYREKVRLATKMPSWLVNSREDMDRFLD
                     TQLERLQADHIDFYLLHSLNAHCWQNYQELKVFDWAERKMAQGKFQHLGFSFHDSYNV
                     FEDILNGYDNWTFCQIQYNYMDINAQAGQKGLRTAANKGLAVVVMEPLRGGRLAKNPP
                     PPAVAEVFARSAKDWTPANWALQWVWNQPEVSLLLSGMSSLEQVEQNLVSAEQSNPNS
                     LAPEELAVIGQARLAREALAPIPCTHCEYCLPCPSGVAIPSVFEIYNEALMYDEPRRG
                     QNAYQNDIKDEHKADSCVECGTCETLCPQQIEIISWLKKAHAYLSPTGS"
     gene            1231..2106
                     /locus_tag="PKL60_04770"
     CDS             1231..2106
                     /locus_tag="PKL60_04770"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNW95484.1"
                     /translation="MAEESPRLPEPDRQLKPANSAQPAASASVPKKERVWFLALGFSA
                     VLLGAGLYLLAPQGFWPRLSAIGYAVCHQIPERSFFAHTHQFPLCARCTGMYLGALLS
                     LFFHLLRGRQRKFAPPALLVILGLLFMAFALDGLNSFAQAAIPLSTPYQTTNLLRLIT
                     GFGAGICIGAVLAPVFNQSVWSNSKPGAALESLPHLLGLLGAAALLGWAVYSGPEWLK
                     AVLAVLSALAVPLVLTLAYTVLGVMLLGRANQYKNWRELRVPLALGLCAALAQILLLD
                     ILRLLLTGTWAALKL"
     gene            2518..3099
                     /locus_tag="PKL60_04775"
     CDS             2518..3099
                     /locus_tag="PKL60_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNW95485.1"
                     /translation="MGNVITGILGAFGLSASAGLNAYIPLLVVSLLAKFTNLIELSKP
                     WDALTSWWTIGVLVVLVLVEAVADKVPAVDHVNDAIQTFIRPTAGAIVFAASAGSVTE
                     VSPVLSLICGLLVAGTVHTVKSAAVRPLITASTAGAGDPIVSLSEDVVATLVSVLSVV
                     LPVLMLIVLILLTWALVSLIRRLRKPVKEYNQE"
     gene            3120..3452
                     /locus_tag="PKL60_04780"
     CDS             3120..3452
                     /locus_tag="PKL60_04780"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNW95486.1"
                     /translation="MTTNNQLPQPITTSPWSIVSLISGISSYFLLPFLGGIIALITGY
                     VAKREIKSSNGALSGSGMATAGLWLGWINIILCVLAVILIILLVVGVIGGFAGCGPLS
                     NWINSFGY"
     gene            complement(3485..3964)
                     /locus_tag="PKL60_04785"
     CDS             complement(3485..3964)
                     /locus_tag="PKL60_04785"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HNW95487.1"
                     /translation="MTAEARFVQVTKLNARGDFVFSYPARLLREQDGARLVAGTFNIE
                     SVAVGKLYFRRGDHALEWYSTRRWYNLLQVCEQASKQVKAWYCNICLPADFREDEICW
                     RDLALDLLVYPDGSMELLDQDEFAELALNWQTRAACWRALKEVMRLFKENSLPFQEG"
     gene            complement(3961..5007)
                     /locus_tag="PKL60_04790"
     CDS             complement(3961..5007)
                     /locus_tag="PKL60_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95488.1"
                     /translation="MLEIRDIHKSYEGKPLLEGISFTVREGETVCLLGESGSGKSTLL
                     RIIAGLEEAEAGSLWWNGEEISAVPAHKRGFGLMFQDYALFPHRNVQANVAFGLRMQG
                     LPSAEVEARTRAALAMVRMDGFEGRAVAELSGGEQQRVALARALAPDPRLLMLDEPLG
                     ALDHALRKQLIGELRRILHDSRKPAIYVTHDREEAFGLADTLMLLHGGRIAQAGSTAE
                     LFRHPVNAWAAGFLGLGSLLSGVVLSLAPLRVGTALGVFRVSDCARAWQPGEKVQLLL
                     RPREARVFAGQPEGENQFTARVRDCLFMGENYQTELRVDDYSLTIQTELPLGVGADLV
                     VGFPPDKLSCLEAE"
     gene            complement(5007..6704)
                     /locus_tag="PKL60_04795"
     CDS             complement(5007..6704)
                     /locus_tag="PKL60_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNW95489.1"
                     /translation="MKAKRGKFWWKALLWLLPAAFLLVFFYQPLAAILRLAFSARLAG
                     EGQSFQPAMVAETFGFTLWQALLSTLLTLGVGLPAAYLFSHFEFPGRKFLRLITLLPF
                     ILPTVVTAVCFNSLLGPRGWLNLGLMALFNLSEPPVHILDSLPAILLAHVFYNSSLII
                     RIVGGAWSRLDRKLTQAARMLGADAWHTFWELTLPLLLPAIGGASLLVFLFDFTSFGV
                     VLMLGGPRFATLEVEIYTQALYLFNLPLAGVLSVVQLAFTLLVAWLQNRVSLPRYGKA
                     AMSSERANLRRPKTRAEKAFTLAVVIMLAALTLLPLLSLVTRSVVSLEADRGARGEVQ
                     AGFTLRYYRELFRNTSGSIFYVPPVTAVRNSLLYAGAAMLIANLLGLLTSYALQASGR
                     LGRWLEPLFILPLGASAVTLGLGFVMVFRRPPWDAFSFPMLIPFAHALVALPLVLRTL
                     LPTLRGIPDSLRQAAGTLGAGWARVFREVDLPVMLRSLLVSMVFAFTVSLGEFGASSF
                     LSTGQNPTIPVAIFRYISQPGALNYGQALAMSTILLLVCAAGSVLIEKARLPGEELF"
     gene            complement(6710..7783)
                     /locus_tag="PKL60_04800"
     CDS             complement(6710..7783)
                     /locus_tag="PKL60_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNW95490.1"
                     /translation="MKIKTLLAGLFTAALLTACAGQTPAAPAQGAETRELTVMTHDSF
                     AVSAGVIAQFEAENNVKVNFVKGGDAGAALNRLILTSLSGKPEADVFFGLDNTFLSRA
                     LENDLLEAYEAPALAEIPESLRLDPQNRALPIDYGDVCINYDRAWFADHSLPLPQSLT
                     DLTKPIYKGLLVVENPATSSPGLSFFLASVAEFGESGWQAWWQALKNNGVVIVSDWER
                     AYYTNFSGSSGRGSQPMVVSYASSPAAELVYAATPLEEAPTGSIVADGTCWRQIEFAG
                     ILKGTTQRALAEKFIDFLLSKPFQEDVPLQMFVYPVLPGAALPEVFTRASAVPVRPAS
                     LDPGLIAERRDEWLRAWTDLMLK"
     gene            complement(7773..8447)
                     /locus_tag="PKL60_04805"
     CDS             complement(7773..8447)
                     /locus_tag="PKL60_04805"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNW95491.1"
                     /translation="MVAHKRALLFANGEARSPQQLRLQADDYLVAVDGGLRHLRTLGL
                     KPHLLVGDFDSISAQELEECRLEGVEILRYPSQKDQTDLELALVAALGRGYRKIVIAF
                     GLGGRLDHSLGNLALLSRPDCGEAVLRFDDGGMEVFLARGVCRTACQPGDIVSLLAWN
                     GAAYDITTAGLAYPLSGESLQPWQTRGISNLCTGLEFEISCGEGALLVIHQRQGIRGL
                     EDENED"
     regulatory      complement(8475..8585)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8912..10984
                     /locus_tag="PKL60_04810"
     CDS             8912..10984
                     /locus_tag="PKL60_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95492.1"
                     /translation="MDENAVSTPASTRLSALARKLWFPLFLFLLTILTYGLLTSRQGF
                     YLDDWYIILFKEKLGNEGFLLYFSKDRPFLSYPYVVLMALFGESAVSWAIFALVMRWV
                     ATCTFWQLLNKLFPEKKKLWMWAAVLFAVYPGFRFHWFAIMYAFAYIFLTFYFLSFLS
                     MAKALENRSAPALYGLWTLLGLLFLLIGIVPQEYFFGMELFRPVLLWILMKRAHPDKP
                     KTVLRESFLHWLPYLAVLVGFSAYRILQSSTYAYQVGVLDQLSAAPLPTLLELAKKAL
                     LSVADALALSWWKAFKLVAQNRFLPGVIPLLAVILSGLALVLVGLFPRKKGEGLPFRE
                     RQGWQLMLAGLFLCFVSLIPFYAGGFEVSSDFPWNRFLLALTPGVAIFLTGALEQAFR
                     SDRLKVVLLALVCSVAIGSQYLTSLDFVKNWERQKDFFWQLSWRAPGLEPGTALVTSE
                     ISFGKYFSGTALNGPLNLIYSPTDSSENINNIILLLDGPQKEYVPAYVAGLPIEYNLR
                     GLQFRGNTDKLLVFSMPAENCLRVLAPTELPDDEVSARKPSLWQGLTDISNLQTIITD
                     LEEPALPPERFFGPENRAQWCYYFEKADLARQQQDWAAVIQLYDKALKLDLGPNGTAE
                     YVPLFEAYLRSDQVEKAYQLHSQLFDWFPGDRAHWCTLFWNVLADQDLSTTDQALLTE
                     MLTQEGCR"
     gene            11013..11987
                     /locus_tag="PKL60_04815"
     CDS             11013..11987
                     /locus_tag="PKL60_04815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW95493.1"
                     /translation="METEETKPTCSVVIPVYNSERSLPLLVQRLHAVLPSLCSQYEII
                     LVNDDSRDGSWAAVQALCAADPRVLGIDLMRNFGQHNALLCGIRQARYELILTMDDDL
                     QHPPEEVYKLFARLAEGADVVYGAPSIEKHGFWRDLSSRLIKKLLHRLMNIKSAATVS
                     AFRLFRAEICKAFADYKGAFVSIDVLLSWGTTRFASVLVNHQEREHGKSNYSFRKLMN
                     HALTLITGFSNAPLRFASLTGFIFTAFGILVLLYVLISYIVRGGSLPGFPFLASIISI
                     FSGVQLFALGIFGEYLGRMFIRTLNKPVYTVRRMLNEPQPVRETKEQA"
     gene            11984..12733
                     /locus_tag="PKL60_04820"
     CDS             11984..12733
                     /locus_tag="PKL60_04820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNW95494.1"
                     /translation="MSANAAAPAPAELSPLDEARFGVRSARAREVSAENLPDVLDFCA
                     AHDVEFLVARCPTTDISAVHALETAGFHLMDTLLYLRFDLKKTPIPPNESDVRIRPAR
                     PDEVEQVGALAFSAFEHFHGHYHADPRLDPRKSTEVYVSWAQRCCTEPSAASLVLVAE
                     AEGRLLGFRALRVNSPTQGEFILAGVNPEYQRRGIYRAFIVEGLRWCQERGLQEVLNS
                     THVANVAVQRACVRAGFEPAYSWYTFHKWFQ"
     gene            12818..13951
                     /gene="rffA"
                     /locus_tag="PKL60_04825"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             12818..13951
                     /gene="rffA"
                     /locus_tag="PKL60_04825"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNW95495.1"
                     /translation="MSKIPFNKPPFLGNEFEYMRAAIESGHISGDGDFTKRCSAKLEG
                     LLGASKVLLTTSCTHALEMAALLLDIQPGDEVIFPSFTFVSTVNAFVLRGARPVFADI
                     RADTLNLDETRLERLLSPKTRALVPVHYAGVGCEMDAILSFAAEHKLAVVEDNAHGLT
                     GTYKGKALGSFGCMATQSFHETKNFSCGEGGALVINDPSYSERAEIIREKGTNRSRFF
                     RGMVDKYTWVDIGSSYLPSDMLAAYLWAQLEQWQTIQARRKQLWEKYDSALRDWAARN
                     GVRQPFIPPDRGQAYHMYYLLFPTLAGRQAFIARLKEQEILSVFHYLPLHLSDMGLRF
                     GGKVGDCPVTECISDQLVRLPFFYNLTDAEQERVIEAVLSQAV"
     gene            complement(14014..>14214)
                     /locus_tag="PKL60_04830"
     CDS             complement(14014..>14214)
                     /locus_tag="PKL60_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95496.1"
                     /translation="SGQQNDKNTQPERSRRLWTIKEGFFAEARPLQGSRMTRTHSLSA
                     AEGSGLLKGDSSLRHWHFRASE"
BASE COUNT         2887 a         4433 c         3971 g         2923 t
ORIGIN      
        1 gtctgtaggc tcagccatgt ctgaatttta actggtatca ggggaatatg caaaagagct
       61 gaaaacctcg aatttccagt tttgaaggta aaattcaggc aattaccatc aaaaggatat
      121 ccgtatgaaa tatcgtccct tcggcaaatt ggattggcaa gcttccgcgc ttggctttgg
      181 ctgcatgcgc tttcccaccc tcgagggtga cagcggctta atcgatgaag accaggctgc
      241 gcccatgctg cgccgcgcca ttgatgccgg cgtaaactac atcgataccg cctggtcgta
      301 ccaccgcgaa caaagcgagc gctttgttgg caaggttttg gcgcggggtt accgcgagaa
      361 agtgcgcctg gccaccaaga tgccttcctg gctcgtcaac agccgcgaag acatggacag
      421 gtttctggac acgcagctgg aacggctgca ggccgaccac attgattttt acctgctgca
      481 cagcttgaac gcccactgct ggcagaacta ccaggagctg aaggtctttg attgggcgga
      541 gcgcaagatg gcgcagggga aattccaaca tctcggcttt tcgttccacg acagctacaa
      601 cgtatttgag gacattctga acggctacga caattggacc ttctgccaga ttcagtacaa
      661 ttacatggat atcaacgcgc aagccgggca aaaaggcttg cgcaccgccg cgaataaagg
      721 tctggcggtg gtcgtgatgg aaccgctgcg cggcggccgg ttggccaaga acccgccgcc
      781 tcccgcggtc gcggaagtct tcgcgcgctc cgctaaagac tggacgccag caaactgggc
      841 cctgcagtgg gtctggaatc aaccggaggt ctccctgctg ctttccggga tgagcagcct
      901 ggaacaagtc gagcaaaacc tggtcagtgc cgaacaatcc aacccgaaca gcctggcgcc
      961 cgaagagctg gcagtcatcg ggcaggcgcg cctggcccgg gaagccctcg cgcccatccc
     1021 ttgcacgcac tgcgaatact gcctgccctg ccccagcggc gtagccatcc cctctgtctt
     1081 cgagatttac aacgaagcgc tgatgtacga tgaaccccgc cgcggtcaaa acgcatacca
     1141 aaatgacatt aaagacgagc acaaagcaga cagctgcgtg gaatgcggaa cctgcgaaac
     1201 gctctgcccg cagcagattg aaatcatctc atggctgaag aaagcccacg cttacctgag
     1261 cccgaccggc agctgaaacc ggcaaacagc gcccaaccag ccgcgtctgc cagcgtgccg
     1321 aaaaaagaac gcgtctggtt tttagccctg ggattcagcg cggtactgct gggtgcaggc
     1381 ctgtacttgc tcgcgccgca gggcttttgg cccaggctca gcgctattgg ctatgcagtc
     1441 tgccatcaga ttccggaacg ctctttcttc gcgcacacgc accaatttcc tttgtgcgcg
     1501 cgctgtaccg gcatgtacct cggcgcgctg ctcagccttt tctttcatct cttgcgcggc
     1561 agacagcgca agttcgctcc gcccgcgctg ctggttatcc tgggtctgct tttcatggcc
     1621 ttcgcgctgg acgggctgaa ctccttcgcg caagccgcaa ttccgctatc aaccccatac
     1681 caaaccacca atcttctccg cctcatcacc ggttttggag cgggcatctg catcggggct
     1741 gtacttgctc cggtctttaa ccagtcggtc tggtcgaatt caaagcccgg cgccgccctg
     1801 gaaagtcttc cgcacctgct cgggctgctg ggtgcggccg cgctgctggg ttgggcagtt
     1861 tattccggtc cggagtggct gaaagccgtg ctggccgtgc tttccgcctt ggccgtgccc
     1921 ctcgtgctca cgctggcgta caccgttttg ggggtcatgc tgcttgggag agcgaaccaa
     1981 tacaaaaact ggcgggaact gcgtgtaccg ctggcgctgg gcttatgcgc cgcgctggct
     2041 caaattttgc ttctggatat cctgcgcctg ctgctcaccg gaacctgggc agccttaaag
     2101 ctctgattat cttgttaaag ctaaatacaa atgtcgtaat gcgggcaagt tagaaatgcc
     2161 ctgatgcgtg gataaagtca tcaggacggt tttttcctcg agaccaagcc tttcttcttt
     2221 tccagcatgt ttcacggttg cgccctttcc cagcggcaag cggctcgatt ttgcctcggt
     2281 caagccgctg gcaaagaaaa aaacggggaa aggctgctac ttccttgtgt gtcaggggat
     2341 tttcattccc ttaaaaaaag aatgttgatt tcctggctct aaatgaggac atttctgtta
     2401 tcctatttca gggcaatata cctttgctct tacaaacctc tgattctcta ctgcgttctg
     2461 gtcataaatg gctataatta atttgtatgt gcaggttttt tatagacaag gaaatccatg
     2521 ggaaatgtta ttacaggtat cttaggggct ttcgggctct ccgcttcggc gggtctgaac
     2581 gcgtacattc cgctgctggt ggtgtccctg ctggcaaaat tcacgaacct tattgagctc
     2641 tccaaaccgt gggacgcgct caccagttgg tggacgattg gtgtgctggt agtgctggta
     2701 ctggttgagg cggtcgcgga taaagtgccg gcggttgacc atgtcaacga tgccatccag
     2761 accttcatcc gcccgacagc tggcgcaatt gtttttgccg ccagcgccgg ctccgtaacc
     2821 gaggttagcc cggtgctctc gctgatctgc gggctgcttg tcgccggaac agtgcacacg
     2881 gtcaagtctg cggcagtccg tccgctcatc accgcctcca cagccggcgc cggcgatccg
     2941 attgtcagtc tctcagagga tgtggttgca acgttagtat ccgtgctttc cgttgtcctg
     3001 cccgtgctga tgctgattgt acttattctg ctcacctggg cattggtatc actcatccgc
     3061 aggctccgaa agcctgtgaa ggaatataat caggaataag gttaacgaaa ggaaaaacta
     3121 tgaccacaaa taatcaattg cctcaaccta tcaccacgtc cccctggtcg attgtcagtc
     3181 tgatcagcgg catctcaagc tattttttgc tgcccttcct gggcggcatt atcgccctca
     3241 ttaccggtta cgtcgccaag cgggaaataa aaagcagcaa cggcgcgctc agcggcagcg
     3301 gcatggctac cgcgggtctc tggctcggat ggattaacat catcctgtgc gtgctggcgg
     3361 tgattctaat cattctactg gtcgtcggcg tgattggcgg ttttgccggc tgcggaccgc
     3421 tttcgaactg gattaatagt ttcggatact aaaattccgg cgctggctca ggcatgtaat
     3481 ttgctcagcc ttcctgaaaa ggaaggctgt tttccttaaa cagccgcatc acttccttta
     3541 gcgcccgcca gcacgcggcc cgtgtttgcc agttcagcgc cagctccgcg aactcatcct
     3601 ggtccaataa ttccatgctt ccgtcaggat ataccaacaa atccagcgcc aggtcgcgcc
     3661 agcagatttc atcttcgcga aagtctgcgg gcaggcaaat attgcagtac caggctttca
     3721 cctgcttgct ggcctgctcg cagacctgca gcaggttgta ccagcgccgc gtcgaatacc
     3781 actccaaggc atggtccccg cgccggaaat acagttttcc aaccgcgacg ctttcgatat
     3841 taaaggttcc tgccaccaga cgcgcgccgt cctgctcccg cagcagccgc gcggggtaag
     3901 agaaaacaaa atcccctcgc gcgttcagct tagtcacctg tacgaagcgc gcttcagccg
     3961 tcattccgcc tccaggcagc tgagtttgtc cggcggaaac ccgacaacaa ggtccgcgcc
     4021 aacgcccagc ggaagctcgg tttgaatagt caaggaatag tcatctacgc gcagttcggt
     4081 ttggtaattc tctcccataa acaggcagtc tctcacccgc gccgtaaatt gattctcgcc
     4141 ttccggctgc cccgcgaaca cccgcgcctc tcgcggacgc agcagaagct gaactttctc
     4201 gcccggctgc catgcccgcg cgcaatcgga gacccggaag acccccaatg ccgtgcctac
     4261 ccgcaggggc gccaggctta aaaccacccc ggaaagcagg ctgccaagtc ccaaaaagcc
     4321 tgccgcccag gcgttcaccg gatgtctaaa aagctcggcc gtactgccgg cttgcgcaat
     4381 cctgccgccg tgcagcagca tcagcgtatc cgccaggcca aaagcctcct cgcggtcatg
     4441 cgtcacgtaa atggcaggtt tgcggctgtc gtgcaaaatt cggcgcagct cgccaatcag
     4501 ctgtttacgc aaggcatggt ccagcgcccc caaaggttca tccagcatca gcaaacgcgg
     4561 gtcaggcgcc agcgcgcgcg ccaaggccac gcgctgctgc tccccgcccg aaagctcggc
     4621 aaccgctctt ccttcaaaac catccatgcg caccatggcc agcgcggcgc gcgtgcgcgc
     4681 ttcgacttct gccgagggca gcccttgcat gcgcagcccg aatgctacgt tcgcttgcac
     4741 gttccggtgc ggaaagagcg cgtaatcctg gaacataagc ccgaagccgc gcttatgcgc
     4801 cggcacagcg ctgatttcct caccgttcca ccacagcgaa ccggcctccg cctcttccag
     4861 cccggcgata atccgcagca gcgtgctctt gccgctgccc gactccccca gcaagcagac
     4921 ggtctcgcct tcgcgcactg tgaaggaaat cccctccaga aggggtttcc cctcgtagct
     4981 tttatggata tccctgattt ccagcatcag aacagctcct ctccgggcag gcgcgccttc
     5041 tcgataagca cgcttccggc cgcgcaaacc agcagcaata tcgtggacat cgccagcgcc
     5101 tggccgtaat tcagcgcgcc cggctgggaa atgtagcgga aaatggccac cgggatggtc
     5161 gggttttggc cggtgctcaa gaagctcgag gcgccaaact cccccagcga gacggtaaaa
     5221 gcgaacacca tgctcacaag cagcgaacgc agcatcaccg gcaaatccac ctcgcggaag
     5281 acgcgcgccc aaccagcgcc caacgtgccc gcagcctggc gcagagaatc aggaatgccg
     5341 cgcagggtcg gcagcagcgt gcgcagaacc aggggaagcg ccaccaacgc gtgcgcgaag
     5401 ggaatcagca tcgggaagct gaacgcgtcc cagggcgggc ggcggaaaac catcacaaag
     5461 cccagcccaa gcgttaccgc gctggcgccc agcggcagga taaaaagcgg ttccaaccag
     5521 cggcccagcc tcccggaagc ttgcagcgcg tagctcgtga gtaagcccaa cagattggca
     5581 atcagcattg ctgccccggc gtagagcaaa gagttgcgca ccgcagtcac cggcggcacg
     5641 tagaatattg agccgctggt attgcggaac agttcgcggt aataacgcag ggtaaacccg
     5701 gcctgcacct cgccgcgcgc gccgcggtcc gcctccaggc tgaccacgct gcgcgtcacc
     5761 aggctgagca gcggcagcag cgtcagcgca gccaacatga tcacaaccgc cagcgtgaaa
     5821 gctttttccg cgcgggtttt gggtcggcgc aggttggcgc gttccgagct catagctgcc
     5881 ttgccgtaac gcggcaggct gacgcggttt tgcagccagg cgaccagcag cgtgaacgcg
     5941 agctggacga cggagagcac acccgccagc ggcagattga acaggtacag cgcctgggtg
     6001 tagatttcca cttccagcgt ggcaaagcgc ggcccgccaa gcatcagcac caccccaaag
     6061 ctggtaaagt cgaacaggaa aacgagcagg ctggctccgc cgatagctgg cagcagcagg
     6121 ggcaaagtca attcccaaaa agtatgccag gcgtcggcgc ctaacatccg ggcggcctgg
     6181 gtcagtttgc ggtccagccg gctccaggct ccgcctacaa tgcgaataat caatgaactg
     6241 ttataaaaca catgcgccag caagatggcc ggcaggctgt ctaagatgtg cacaggcggc
     6301 tcggaaagat tgaagagcgc catcaagccc aggttcaacc agccgcgcgg tcccagcagg
     6361 ctgttgaaac acaccgccgt cacgacggtt gggaggatga agggcagcag cgtgataagc
     6421 cgcaggaatt ttcgtccggg gaactcaaaa tgggagaaca ggtacgcagc cggcaagccc
     6481 acccccagcg tcagcagcgt ggaaagcaaa gcctgccaga gcgtgaatcc gaaagtttcc
     6541 gcaaccatcg cgggctgaaa actttgcccc tccccggcca gccgcgctga aaacgccagg
     6601 cgcagaattg cagccagcgg ctggtaaaaa aacaccagca gaaaggctgc cggcagcagc
     6661 cacagcagcg ctttccacca aaacttgccg cgcttcgcct tcattcagct catttcaaca
     6721 tcaaatcggt ccaggcgcgc agccactcgt cgcgccgctc cgcgatgagc ccggggtcca
     6781 ggctggctgg ccggacaggc acagcgctcg cgcgcgtgaa cacctcgggc agcgcggcgc
     6841 cgggcagcac cgggtaaacg aacatctgca gcggcacatc ttcctgaaac ggcttgctca
     6901 gcaggaagtc gataaatttt tccgccaggg cgcgctgggt tgtccctttc aggatgccgg
     6961 cgaattctat ctgccgccaa caggtcccgt ctgccacgat agagccggtt ggggcttcct
     7021 cgagcggcgt cgcagcataa accagttcag ctgccgggct ggaagcgtac gaaacaacca
     7081 tcggctggct tccccgtccg gatgaacctg aaaagttggt gtaatacgcg cgctcccaat
     7141 cggaaacgat taccacgccg ttatttttca gcgcctgcca ccaggcttgc cagccgctct
     7201 ccccaaattc cgccacgctt gccaggaaga aggacagccc gggcgaggag gtggccgggt
     7261 tctcgaccac cagcagaccc ttgtagatgg gcttggtcag gtccgtcaaa gactgcggca
     7321 gcggcaggga atgatccgcg aaccaggccc tgtcgtagtt gatgcacaca tcgccgtagt
     7381 caatgggcag ggcgcggttt tgcgggtcca accgcaagct ttccgggatt tccgccagcg
     7441 cgggcgcctc atatgcctct aacaggtcgt tttccagcgc gcgggagagg aaggtgttgt
     7501 ccagcccgaa aaacacatcg gcttccggtt taccggacaa gctggtaagg attagccggt
     7561 tcaacgccgc gccggcatct ccgcctttga caaagtttac cttgacgttg ttttcggctt
     7621 caaactgcgc gatgaccccg gcgctgaccg cgaacgaatc gtgcgtcatc acggtgagct
     7681 cccgggtctc cgcgccttgg gccggcgcgg cgggggtttg tcctgcgcag gcagtcaata
     7741 aagcagctgt gaacaagccc gccagcagag ttttaatctt cattttcgtc ctccaaacct
     7801 cgtattccct ggcgttgatg aatgaccagc agcgcgccct cgccgcacga gatttcaaac
     7861 tcgaggccgg tgcacaggtt gctaatcccg cgcgtctgcc agggctgcag gctctccccg
     7921 ctcaatggat acgccagacc tgcagtcgtg atgtcgtatg ccgcgccgtt ccacgccagc
     7981 agcgagacga tatcgcccgg ttggcaggcc gtcctgcaca ctccgcgcgc caaaaatacc
     8041 tccatcccgc cgtcgtcaaa gcgcagtacc gcttctccac aatcggggcg ggaaagcagc
     8101 gccaggttgc ccaggctgtg gtccagcctg ccgcccaggc cgaaggcaat cacaatcttg
     8161 cggtaccccc gcccaagcgc cgcgaccagc gccagttcca ggtcggtctg gtctttttgg
     8221 ctggggtagc gcaggatttc caccccctcc aggcggcact cttccaattc ctgcgcgctg
     8281 atcgaatcaa aatccccgac cagcagatgc ggtttcaagc caagcgtccg gagatggcgc
     8341 agtccgccgt ccactgcgac gaggtaatca tccgcctgca atctcaattg ctgcgggctg
     8401 cgggcttccc cattggcaaa gagcagcgct ctcttgtgcg cgaccataaa aaacaccctt
     8461 ccgccttggg agggtgcctg aagcgcattc attccctccg ccggcattat ccggatcagg
     8521 ttctgcgggt cagaagctca ctgcttccct ctcagcctga aaaacaggct ccccgtgtgc
     8581 ttaccctttt atcataccat caaattcaga gattccgggc attgtagcac ctgcccgaaa
     8641 gtcggcagta gcagggcagt cagggcgtca gccggtagtt gtccggattc ccgggttcgg
     8701 agcgcagctc aatcgaattt tgttgcgttg atacagcgag gaagcgaata aaatccgccg
     8761 cacccgctcc actcgcttcc gcgcggtcag caaaacccgg gcggctcggc tggggacgga
     8821 agcggagaat aacagcgcgt ttcttgatat aatcccacag caggcttgca ggctgagcct
     8881 ggatactctc tgccgtggga atgacctatt catggacgaa aacgccgtca gcacacccgc
     8941 aagcacccgt cttagcgccc tcgcgcgcaa gctgtggttc ccgctcttcc ttttcctgct
     9001 cacgatcctc acttacgggc tgctaacctc ccgccagggc ttctacctgg acgattggta
     9061 catcatcctc ttcaaggaaa agctgggcaa cgaaggcttt ttgctctatt tttccaaaga
     9121 ccggcctttc ctctcatatc cttacgtcgt cctgatggcg ctctttggcg aatccgccgt
     9181 aagctgggcg atttttgcgc tggtgatgcg ctgggtggcc acctgcacct tctggcagct
     9241 gctgaacaag ctcttccccg agaaaaagaa gctctggatg tgggccgcgg tgctcttcgc
     9301 ggtctacccg ggtttcaggt tccactggtt cgcgattatg tacgccttcg cgtacatctt
     9361 cctgaccttt tacttcctgt cgtttctcag catggccaag gcgttggaaa acaggtctgc
     9421 gccggcgctc tacggcttgt ggacgctgct cggactgctc ttccttttaa tcgggattgt
     9481 cccgcaggag tattttttcg ggatggagct tttccgtcca gttctgctgt ggattctgat
     9541 gaaacgcgcg caccccgaca agcccaagac cgtcctgcgc gagagcttcc tgcattggct
     9601 gccgtacctg gcggttttgg tgggctttag cgcctaccgg atcctgcaga gcagcaccta
     9661 tgcctaccag gtcggcgttc tggaccagct ttccgccgcc ccgctgccca ccctgcttga
     9721 actggcgaaa aaggcactcc tctccgtcgc tgacgcgctg gcgctctcgt ggtggaaggc
     9781 tttcaagctg gtggcgcaaa accgcttttt acccggggtc attccactgc tggctgtgat
     9841 tctttccggt ctggcgctgg tcctcgtggg gctcttcccg cgcaagaaag gcgaagggct
     9901 ccctttccgg gagcggcagg gctggcagct gatgttagcc gggctgttcc tgtgctttgt
     9961 atccctcatc cccttctacg ccggcggctt tgaggtctcg tccgacttcc cgtggaaccg
    10021 ctttttgctc gcgctgaccc ccggcgtggc aatcttcctg accggcgcgc tggaacaggc
    10081 gtttcgctcg gaccggctga aagtggtgct gctggcgctg gtgtgctcgg ttgctattgg
    10141 ttcgcagtac cttacctcgc tggatttcgt gaagaactgg gagcgccaaa aggatttctt
    10201 ttggcagctc agctggcgcg cgcccgggct tgaacccggc accgcgttgg tcacttccga
    10261 gatttccttc ggaaaatact tcagcggtac cgccctgaac ggaccgctca acctgattta
    10321 ctctcccacg gacagcagcg agaacatcaa caacatcatc ctgctgctgg acggtcccca
    10381 aaaggaatac gtgcccgcat acgtcgcggg gctgcccatt gaatacaacc tgcgtggctt
    10441 gcagtttcgt gggaacaccg acaagctgct ggttttctcc atgcccgctg aaaactgcct
    10501 gcgcgtcctc gctcccacgg agctccccga cgacgaagtc tccgcccgaa agccgagcct
    10561 gtggcagggt ctgacggaca tatccaatct gcaaaccatc attaccgacc tcgaagaacc
    10621 cgctctgcct ccggaacgct tttttggccc ggaaaaccgc gcgcaatggt gttattattt
    10681 tgagaaagct gacctggcgc gccagcagca agattgggcg gcggtcattc agctttacga
    10741 caaggccctc aaactggacc tgggacccaa cgggacggcg gagtatgtgc cgctctttga
    10801 ggcttatctg cgctcagacc aggtcgaaaa agcttaccag cttcacagcc agctcttcga
    10861 ttggttcccg ggcgaccggg cgcattggtg cacgctgttc tggaatgtgc tggcggacca
    10921 ggacctgagc acaacggacc aggcgctcct aacggagatg ctcacgcagg aaggctgccg
    10981 ctgaaaacca aaaggaggac agacaagccg ccatggaaac tgaagaaaca aaacccactt
    11041 gctcggtagt catcccggtc tacaacagcg agcgcagcct gccgctgctg gtgcaaaggc
    11101 tgcatgccgt cctccccagc ctgtgctcgc agtatgaaat catcctggtg aacgacgaca
    11161 gccgcgatgg cagttgggcc gccgtccagg cgctttgcgc agccgaccca cgcgtgctgg
    11221 gcatcgacct gatgcgcaac ttcggccagc acaatgccct cctgtgcggc atccgccagg
    11281 cgcgttacga actaatcctg accatggatg acgacttaca gcacccgcct gaagaggtgt
    11341 acaagctctt cgcgcgcctg gctgagggcg cggatgtcgt ttacggggcg ccgtccatcg
    11401 aaaagcacgg cttctggcgg gacttatcct cccggttaat caagaaactg ctgcaccggc
    11461 tgatgaacat caagagcgcg gcaacggttt ccgcgttccg cctgtttcgc gcggagattt
    11521 gcaaggcttt cgcggattac aagggcgcgt tcgtctcgat tgacgtgctg ctcagttggg
    11581 gtaccacgcg ctttgcgtcc gtgcttgtca atcaccagga acgcgaacac gggaaatcga
    11641 attacagctt cagaaaatta atgaatcacg cgctcacgct cattaccggt ttcagcaacg
    11701 cgccgctgcg ctttgccagc ctgaccggtt ttatttttac tgctttcggt attctcgtct
    11761 tgctttatgt gctcatcagc tacattgtgc gcggcggcag cctgcctggt ttcccatttc
    11821 tggcctctat catttccatt ttctcgggcg tacagctctt cgcgctgggg atttttggcg
    11881 agtacctggg acgaatgttc atccgcacgc tgaacaagcc ggtttacacc gtgcgccgga
    11941 tgctcaacga accccaaccc gtccgcgaaa ccaaggagca ggcgtgagcg ctaatgcagc
    12001 cgcccctgcc ccggcggagc tttctccgct ggacgaagcc cgctttggcg tccgctccgc
    12061 gcgcgcccgg gaagtgagcg ctgagaacct gccggacgtc ctggactttt gcgctgccca
    12121 tgatgtggaa ttcctggtcg cgcgctgccc caccacggat atcagcgccg tccacgcgct
    12181 ggaaacagcc ggatttcacc tgatggacac cctgctctac ctgcgctttg acctgaagaa
    12241 aacccccatc ccgccaaacg agagcgatgt ccgcatccgc cctgcccgcc cggacgaggt
    12301 ggagcaggtc ggcgcgctgg ctttttccgc tttcgagcac ttccatgggc attaccatgc
    12361 cgacccgcgc ctggaccccc gcaaaagcac ggaagtgtac gtctcctggg cgcagcgctg
    12421 ctgcaccgaa ccaagcgcgg ccagtctggt tttggtagcc gaagcggagg gcaggttgct
    12481 gggtttccgc gcgctgcggg tcaactcccc cacgcagggg gaattcattc tggcaggcgt
    12541 gaacccggag taccagcgcc gcggcatcta ccgcgccttc attgtggaag gcttgagatg
    12601 gtgccaggaa cgcgggctgc aggaagtatt aaactccacg catgtggcga atgtggcggt
    12661 tcagcgcgct tgcgtgcgcg cgggcttcga gccggcttat tcctggtaca ctttccacaa
    12721 gtggttccag taaacacctt tggatggaac attctacgcg ggctttcgct tcgcgcaagc
    12781 gattaaaaca gcagcatatt tcagatcaga gcaaaccatg agcaaaatcc ccttcaacaa
    12841 acccccgttc ctcggcaatg agttcgaata catgcgcgcg gctatcgaaa gcgggcacat
    12901 ctccggcgac ggcgacttta ccaaacgctg cagcgcaaaa ctggaggggc tccttggggc
    12961 ctcaaaagtc ctgctgacca cctcctgcac acacgcactg gaaatggcgg cgctgctgtt
    13021 agacattcaa cccggggatg aggtcatctt cccatctttc actttcgtct ccacggtgaa
    13081 cgcgttcgta ctgcgaggcg cgaggccggt tttcgcggat atccgcgcgg acacgctgaa
    13141 ccttgatgaa acccgactgg aacgcctgct cagccccaaa acccgcgcgc ttgtgccggt
    13201 gcactacgcc ggtgtcggct gcgagatgga cgccattctc agttttgccg ccgaacacaa
    13261 gctggcagtt gtggaagata acgcacacgg actgaccggc acttacaaag gcaaagccct
    13321 gggcagcttt ggctgcatgg caacccagag cttccacgag accaagaatt tttcttgcgg
    13381 agaaggcggc gcgctggtca tcaacgaccc cagctacagc gaacgcgcgg aaatcatccg
    13441 cgaaaaaggc accaaccgca gccgcttctt ccgcggcatg gtggacaagt acacctgggt
    13501 ggatatcggc tccagctacc tgccttccga catgctggcg gcgtacctgt gggctcagtt
    13561 ggagcagtgg caaactatcc aggcgcgccg caagcagctt tgggaaaagt acgacagcgc
    13621 tctgcgggat tgggccgcgc gcaacggggt ccggcagcct tttatccccc cggaccgcgg
    13681 gcaggcctac catatgtact acctgctgtt tcccacgctc gccggcagac aggcctttat
    13741 tgcccgcctg aaagagcagg aaatcctgtc cgtcttccat tacctgccgc tgcacctttc
    13801 ggacatgggc ttgcgttttg gcggaaaagt cggggactgc ccggtcaccg agtgcatcag
    13861 cgaccagctg gtacggctgc cgttcttcta caacctgacg gacgcggagc aggagcgggt
    13921 catcgaggcg gtgctcagcc aggcggtctg aacccggcat agaatctgcg cgcacgcaag
    13981 gctgtttgct cttgttcaac ctatctatgc ttgtcattct gatgccctga aatgccaatg
    14041 ccgcagcgaa gaatccccct ttaataatcc agagccttcg gctgcgctca ggctgtgtgt
    14101 tcttgtcatt ctgctgccct gaaggggacg tgcctcagcg aagaatccct ctttaatagt
    14161 ccagagcctt cggctgcgct caggctgtgt gttcttgtca ttctgctgcc ctga
//
LOCUS       DAOQAD010000042        14152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_4687_length_14152_cov_5.874938, whole genome shotgun sequence.
ACCESSION   DAOQAD010000042 DAOQAD010000000
VERSION     DAOQAD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14152
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4687_length_14152_cov_5.874938"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PKL60_04835"
     CDS             <1..726
                     /locus_tag="PKL60_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNW95497.1"
                     /translation="APANRNFSAEGLPPMVVYIHGGPTSAARIGFDLDTAFFTSRGYA
                     YLEVNYRGSTGFGRSYRDALRGNWGKLDLQDAVEGARAVVGMGLADPSRLIIKGGSAG
                     GYTVLNALIHYPGFFKAGLCSYGVSNLFTLDMDTHKFEAHYNASLIGALPEAAQKYHD
                     FSPIFHAHKIQDPLAIFQGADDKVVPQSQSDSIVEVLRANRVPYVYRIYPGEGHGFRK
                     SESIIDYYNTIERFLLQQVIFSV"
     gene            751..1581
                     /locus_tag="PKL60_04840"
     CDS             751..1581
                     /locus_tag="PKL60_04840"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNW95498.1"
                     /translation="MEISETYLKASDGLKLYVRSWSCAGEARAVVVLLHGVGEHCQRY
                     DHVAERFCERGFRVVGYDRRGHGRSDGKQGVIGSVEQLGADFSQIIEHARARNPALPV
                     FLYGHSMGALGVLCYSLHEKPSVKGIIATSPLLDTTTMTKFQKLLVRVLDPILPSLTV
                     PSGLEQAAISRDSAVIEAYKADPLVHDKVSVRLGAYLNNAPEYVMKHAGEWTLPLYLA
                     HGTADRICPISGSDAFASKVAAPITYQRWEGLYHETHNEPEKELVIGTMLDWVEGQLG
                     "
     gene            complement(1759..1845)
                     /locus_tag="PKL60_04845"
     tRNA            complement(1759..1845)
                     /locus_tag="PKL60_04845"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1808..1810),aa:Ser,seq:gga)
     gene            complement(1931..2572)
                     /locus_tag="PKL60_04850"
     CDS             complement(1931..2572)
                     /locus_tag="PKL60_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNW95499.1"
                     /translation="MYEAEKKAVLECALTMEHYRLVSLSGGNVSVRLPDGSFLVTPSA
                     VRYDGMSPDDVVRVDAQGKVVEGHLRPSSDTSALLYVFEHMPEVNAVIHTHQPYANAV
                     GLVTDVLPAFLTLMIDATQKEVHVAPFTISSDEGMGVLSVEYARGALCVILKHHGVIA
                     FGKDLDQALEAAVYLEDAAKVYLLARAIGPVPLLDEKQIADQAEDRGNYGQKK"
     gene            complement(2585..3427)
                     /locus_tag="PKL60_04855"
     CDS             complement(2585..3427)
                     /locus_tag="PKL60_04855"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: protein motif:HMM:NF009689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HNW95500.1"
                     /translation="MPTQLPFPIGIYEKALKLQPLPAMFADAVRAGYDTFELSLDESD
                     ARLARLDWSAARKRETRRAAEESGIRLFSACFSGHRKYALGSADPGTARRAMDLMRKG
                     LDFCVETGIRVLQLTGSDVYYEPASPESARRYAENVACGAEMAGDLGVMLAIEPVDKH
                     IRSIRQALELVREINSPWLQIYPDAANLLVQGFDPLEELEAGQGHLVGLHIRDAVPGN
                     SYNLPFGSGQLDFVSVLRGLRRINFHAPVLIEYWHVGEPDYLERAARAREFLVENFAL
                     AGYA"
     gene            complement(3427..4074)
                     /locus_tag="PKL60_04860"
     CDS             complement(3427..4074)
                     /locus_tag="PKL60_04860"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNW95501.1"
                     /translation="MRVIPSIASGNLLEIGSELQRIQSLPRLHLDMEDGNFLPSMTFG
                     MDMVRAIAGVWKGELDAHLMVTNPFDYLDGMHACGVKSLCAHLEALAYPATFLGRLRA
                     HGMQAGLALNLKTGVDALPYFADQLDYVLFLTSEPDSEGMKLRPAVLSKIAQARGLLP
                     ARVSLWADGGVNAGNIASLRAAGVDTVIVGRSFFSAKDPMAAYAELLEAAEKAGA"
     gene            complement(4093..5604)
                     /locus_tag="PKL60_04865"
     CDS             complement(4093..5604)
                     /locus_tag="PKL60_04865"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNW95502.1"
                     /translation="MQTILGIDNGGSKIKCALFDLQGHEIAVSSRQAPLIIPQPGMTE
                     RDPGLVWRTNVEVIREVLQSSGVNPREIIGIGLTGYGNGMCLVDADGKPTWNCIVSTD
                     TRGSLYAEKFAASGIEREAFAYSHQGFWAAQPAVLLLWFKEHAPEVLERSAWALGIKD
                     FVRLHLTGAAACEFTEASSWGLMNIHTLKFEPGLFALLGLEDCFRLMPPCLSPVAISG
                     YVNAQAAAETGLAEGTPVAGGCFDVDAGALASGVLDGETLCLIAGTWSINEYLAPELR
                     RGYKQTTNSVSHSFLPGHFLVEESTPTSASNFEWFLENFLAAENSGKTRRQLYEHCDQ
                     SVEALPPEESSAVFVPYLFDSATVSGGKGAFFNLSSFYKQGHLIRAVYEGVVFSTVFH
                     VRRLIKDGHAFSKARLSGGVTNSRVWAQMMCDALRLPVEIPEGSELGAKGAAICAGIA
                     CGAFADYQDAAAKMVSIRERLMPCEEQAEIYARKFMLYEKALAALECFHGGAE"
     gene            complement(5654..6460)
                     /locus_tag="PKL60_04870"
     CDS             complement(5654..6460)
                     /locus_tag="PKL60_04870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNW95503.1"
                     /translation="MTDKPGTPLKLLALDLDGTTVDDHGQISDATARSLQLAREKGAA
                     ICFVTGRSEFEVLSLEARFPYADYLVVDTGAKITELATGKLLLHECLDPREAEIVVEN
                     FIRKNRLLHIKAGRFWGATLMDASVEAFARYANYQPVLIRKPEDAPLAEIDSFCVYGQ
                     PSRAAMDRLIERRGLNLYTMHSQPHYYDVLQHGINKWHAVSFLAQRLRIPTEGVIAVG
                     NFSNDVEMIKKSGIGIAVRNASEDAKAAADYITRRSNNRNAIGEVVEMFL"
     gene            complement(6462..7277)
                     /locus_tag="PKL60_04875"
     CDS             complement(6462..7277)
                     /locus_tag="PKL60_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HNW95504.1"
                     /translation="MNWTEEVFHVKKPIISMLHLQPLPGDPLYKESASMRRVIELARA
                     DLHAIQDGGVDGLIFSNEFSLPYQRHMSFVTPSAMARVIGELIHEIRIPYGVDCISDG
                     LATIELSAAVDASFARGTFSGVYVGDGGFYNNELSELLRRKSALRLDKLKLLYFLNPE
                     SDTNLDTRTLTAIARSLVFKASPDALCISGSAAGKGVENELLAQVKESVPQTVVFANT
                     GCNKDTITDKLKICDGAVVATTFKVDGKLFNPVDPERVKAFMKPVHAFRETLN"
     gene            complement(7338..8225)
                     /locus_tag="PKL60_04880"
     CDS             complement(7338..8225)
                     /locus_tag="PKL60_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95505.1"
                     /translation="MDITREELYRWCSLPLEQLQDHSERKVNLILKEDRPAAMRLIGN
                     MMADEILENNRLGKLTKWVLPAGPTDEYDVLIERVNRERISMRNLHVFHMDDFLDWEG
                     RPYPVADTYESLEGTMNAAFYGRIDPDLNVPVEQRHWPRINDLDAIDNLVEEMGGVDT
                     LWAGVGAKGLVAFCEAPRPYTHRITLEEFARSKTRIVNLNDDTIVALSQRSFGACYDR
                     VPPRAVTLGFKAMLTAKRAILMIATGAWKQTVVRVALFSEPTLEYPVTLLPKYIPDVT
                     LICDRNTADHPMSHPIRGW"
     gene            complement(8228..9055)
                     /locus_tag="PKL60_04885"
     CDS             complement(8228..9055)
                     /locus_tag="PKL60_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNW95506.1"
                     /translation="MKISLFTAGISRYPLEKSFELAAKYHYDAIEIGGFRPHAYALDI
                     LLDGGRKIRDLSERYQLPIVSYAPENTGSPYSLVFEDPELNRDSLEYFKRTLDAAKLI
                     GSEYCMFACNHPGYGRLESEVRRLFIENMKVLAEHAEKIGQTIILEPVTPNEGTILVT
                     SDDLYWAIQAVNSPMVKGMLDLACPLLSREPMVEYFKKCGSDIRHIHFIDAAIDSEDH
                     LIPGDGQMDFPGIVRTLKEVGYSGYLSLELFTRYQTEPEYATARGYNVIKALLEEEA"
     gene            9484..10869
                     /locus_tag="PKL60_04890"
     CDS             9484..10869
                     /locus_tag="PKL60_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNW95507.1"
                     /translation="MATQSETQVSPAVGAEGNVLGRKLGFLAVLAIGVGTTIGSGIFS
                     SVGEVANAGGSALMTVLAFLVGGIIMIPQILAYAELSSAYPENGGAYIYFREAGSDFL
                     AFLSGWIGFWGTDTPGISIMALAIANYIGFLTGIEGIWLKLIAIAFIVFYAWTHIRSV
                     EGGSKTQTALTVIKIIPFVLLIAVGLFYLQGSLLSTSPVAGAPTGILALVAAISATTW
                     SYDGMQSAAWVAGEVKNPRKNMPKALISTVLFVTLMYTLISLVITGLLPFDQLTTSTA
                     PVADAMAQIPLIGQAAGIITAIIAILVINGSLTSLILIQPRQQWAMAQDGMWFRSFGK
                     VHPKWQTPVLSIVVQCAFGILFLFFASLRDLLGYFTFALLLRNTATMASIFWLRKKEN
                     YKPSYKMPAWQLMTILAIAFSFILLVSTLLWAPGPSLIAGVIGAVTGYPAYLYFKKTQ
                     KPFETFGNEEA"
     gene            10983..11888
                     /locus_tag="PKL60_04895"
     CDS             10983..11888
                     /locus_tag="PKL60_04895"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNW95508.1"
                     /translation="MAHYFQNLVTELITPFNDDGSVDHETLAAIVDFQIKNGVRHFFV
                     NGLGGESHLLSFQERQDVLRTVSSHCTDGAKTMACVFVSTVREGKELIDMYADVQHDA
                     VCLTAPAYFPYTEQALYAFTSELLEYARQPAYIYNCVQMGTLYCPELLARLAKDHSNL
                     YGYKNATRDIVHYMQCLMRIDATKFDFLDGCDATIAPTMLAGGVGCVSFMGVPFPKET
                     KAICDYALAGNAEKAMQAQFRILELRNILKKSPFNAAYMLAMELSGGPVARNTRMVPE
                     MTLVSDAVRKELFETYARQQAEPMA"
     gene            11933..13027
                     /locus_tag="PKL60_04900"
     CDS             11933..13027
                     /locus_tag="PKL60_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95509.1"
                     /translation="MMNSTKIELNEAAFSEKEKQFLQNEAFTAVLFRYSTQVCAVRLS
                     NSKINLVVLPFQGQQVWRFASPIGNMTMKSIFDEPENTTRFGLNYGAFLIHCGLTANG
                     NPSAEDSHPLHGELPNCHYQEAYLRVGTDEKGDFLALGGSYVYRNSLEYHYEFAPELR
                     MRPDSALVDVHIQARNFRPKAMKYMYMAHINWLPVDCSRLVASANPKATEVFSDTFGL
                     PNSDQQKFMDYIERLGADPTIADKIDSSSQIYDPEVCMYLHYSADEQGYAHAMQVFPG
                     GDAAYVAFRTKELPNAVRWFARTGSEDALAFAIPSTNNHLGFARNDAKGLIREIGAKD
                     CLEMHYRFGYLSCDERRDVETKIDKVLGKA"
     gene            13066..14007
                     /locus_tag="PKL60_04905"
     CDS             13066..14007
                     /locus_tag="PKL60_04905"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNW95510.1"
                     /translation="MTKEIDVIVLNSHGVGQFCNIKRLPFLGETLRAWNWRVEEDGGK
                     GTTVAVSLGRLGVKTAYIGKVGKDPWGDLGFKWMGDAGVDTTYMYQSDEVATGTGLVM
                     IDEDGNNTIVDGDSSLEKLTVEEIRTALEDMKNAKFFITGFAMPQHLALASAKMAHEM
                     GIATVLNPSPLPHEPMGDLSYIDYIVCNEVEGRVLAEFEEGSTEYEKIARRVREIYHC
                     GSIAMTLGEEGCLVLHEDQIYRVPSVKVDVVETVGAGDSFLAAFVANLVWGKSVQEAA
                     AWAVKCSALKVTRAGTIPAFPYLREVEAFIANLDESK"
BASE COUNT         3028 a         4195 c         3911 g         3018 t
ORIGIN      
        1 gcccccgcca accgcaattt cagtgcggag ggtttgccgc caatggtggt ctacatccac
       61 ggcggcccaa cttcggcggc gcgcatcggc tttgacctgg acacagcctt cttcaccagc
      121 cgcggctacg cctacctgga agtgaactac cgcggcagca ccggcttcgg gcgctcctat
      181 cgcgacgctc tgcgtggaaa ctggggcaaa ctggacctgc aagatgcggt ggaaggcgcc
      241 cgggctgtgg tgggaatggg gctggccgac ccgtcgcgcc tcatcattaa gggcggcagc
      301 gcgggtggat acaccgtttt gaacgcgctg attcactacc cgggtttctt caaggcaggt
      361 ctatgctctt acggcgtctc caacctgttc acgctggaca tggataccca caaatttgaa
      421 gcgcattaca acgcctctct cattggcgct ttacctgaag cggcgcaaaa gtatcacgat
      481 ttctccccca tctttcacgc gcacaaaatc caggacccgc ttgcgatttt ccagggcgcg
      541 gatgacaaag tggtacccca aagccagtca gacagcatcg tggaggtgct gagagccaac
      601 cgcgtgcctt atgtctaccg catttacccg ggcgaggggc acggcttccg gaaatccgaa
      661 tccatcatag attattacaa cacgatagag cgttttttat tgcagcaagt aatattcagc
      721 gtatgatccc gggaatagag gaatgcaaac atggagatta gcgaaacata tctcaaagcg
      781 tcagacggcc tcaagctgta cgtccgcagt tggagctgcg ccggggaagc cagggctgtg
      841 gtggtgctgc tgcacggcgt gggggaacac tgccagcgct acgaccacgt ggcggagcgc
      901 ttttgcgaac gcggcttccg cgttgtcggg tacgaccgcc ggggacacgg ccgttcggac
      961 gggaaacagg gcgtcatcgg ctccgtggag cagctcgggg cggatttcag ccagatcatt
     1021 gaacacgcca gggccaggaa tcccgcgctg ccggtcttcc tctacggcca cagcatgggc
     1081 gcgttaggcg ttttgtgtta ctccctgcac gagaagccga gcgtgaaagg aattatcgcc
     1141 accagcccgc tgttagacac cacaaccatg accaaatttc agaagctgtt ggtgcgggtg
     1201 ctggatccca tcctgccgag cctgacggtt cccagcggat tggaacaggc ggccatttcc
     1261 cgcgacagcg cggtgatcga ggcttacaag gctgacccgc tggtgcatga taaggtcagc
     1321 gttcgtctgg gcgcttacct gaataacgcg ccggagtatg tgatgaaaca tgccggtgaa
     1381 tggaccctgc cgctctatct ggcccacggc accgccgacc gcatctgccc gatcagcggt
     1441 tcggacgcgt tcgcgagcaa ggttgcggcg cccatcacct accagcgctg ggaaggactc
     1501 taccacgaga cgcacaacga gcctgaaaaa gagctcgtca tcggtacgat gctggattgg
     1561 gttgaaggtc agttgggctg agcgaacttc ctgttaaatc gggaaaacag catcattttc
     1621 agaaacaaat aaatcatgaa tcaggactcc cgcgtgtgcg ggagtcctga ttttatctgc
     1681 gaaaagataa ggggagttat ctggagaaac cttggtcctc agaccttgtc aaaaaaagtt
     1741 gcgcttaaat aaaaagagcg gagagggcgg gattcgaacc cgcgatacct catcggtata
     1801 ctcgctttcc aagcgagcgc actaggccag ctatgcgacc tctccacaag cagagcgcta
     1861 ttataccaga attctttacg gatttcgcaa gccgatttcc gggtttcgcg ctcgcgattc
     1921 tgtccgatat ttacttcttt tgcccgtaat tcccgcggtc ttctgcctgg tcggcaatct
     1981 gtttttcatc caacagcggc accgggccaa ttgcgcgagc cagcaggtaa accttggcgg
     2041 catcttccag gtaaaccgcg gcctccagcg cctggtcgag gtctttgccg aaggcaatca
     2101 cgccgtggtg cttgaggatg acgcacagcg ctccgcgcgc gtattccacg ctcaaaacgc
     2161 ccatgccctc atccgaggaa atggtgaacg gcgcaacatg cacttctttt tgggtcgcgt
     2221 caatcatcag cgtcaaaaag gcaggcaaaa cgtccgtcac cagacccacc gcgttcgcgt
     2281 aaggttgatg ggtgtggatg accgcgttca cctcaggcat gtgctcgaag acgtacagca
     2341 gcgcggaagt gtcggaagag gggcgcagat gcccttccac cacttttccc tgcgcgtcca
     2401 cccgcacgac gtcatccggg ctcatgccgt cataacgcac cgccgagggc gtcaccagga
     2461 agctgccgtc aggcagccgc acagacacat tcccgccgga cagactgacc agccggtaat
     2521 gttccatggt cagcgcgcac tccaaaacgg ctttcttttc tgcttcgtac atgttcaacg
     2581 ctccttatgc gtaccctgca agggcgaaat tttcaaccag aaattcccgg gcgcgcgctg
     2641 cgcgctccag gtagtcgggt tcacccacat gccaatactc aatcagcaca ggcgcgtgga
     2701 agtttatccg gcgcaggccg cgcaagaccg agacaaaatc caactggccg ctgccaaatg
     2761 gcaggttgta gctatttccc ggaaccgcgt cccggatgtg cagcccgacc aggtgccctt
     2821 gcccggcttc cagttcttcc agcgggtcaa agccctgcac aagcaggttg gccgcgtccg
     2881 gatagatttg cagccagggc gagttgattt cgcgcaccag ctccaacgcc tggcggatgg
     2941 agcggatgtg cttatccacc ggctcaatcg ccagcatcac gcccaggtcg cccgccattt
     3001 ccgccccgca cgccacgttc tccgcatagc gcctggcgct ttccgggctg gcaggctcgt
     3061 agtacacatc cgagcctgtc agctggagca cgcgaatgcc ggtctcgacg cagaagtcca
     3121 ggcctttgcg catcaaatcc atcgcccgcc gcgctgtccc ggggtcagcc gaacccagcg
     3181 cgtacttacg gtgcccgctg aaacaagccg agaacaagcg gattccgctc tcttccgccg
     3241 cgcggcgggt ctcccgcttt cgcgccgcgg accagtccag gcgcgccagg cgcgcgtcgc
     3301 tctcgtccag gctgagttca aaggtgtcgt agcctgcccg gacggcgtcc gcgaacatag
     3361 cgggcagcgg ctgaagcttg agagctttct cataaatccc aatcgggaaa ggaagctgcg
     3421 ttggcattat gcccccgcct tttctgccgc ttcgagcagt tccgcgtaag cagccatcgg
     3481 atctttcgcc gagaaaaacg acctgccgac aatcaccgtg tccaccccag ccgcgcgcaa
     3541 gctggcaata ttgccggcgt tcacgccgcc gtccgcccag agcgaaacgc gcgcggggag
     3601 caggccgcgg gcttgcgcaa ttttggatag cacggccggg cgcagcttca tcccctcaga
     3661 atctggctcg ctggtcagga agagcacata atccagttgg tcagcaaagt aaggaagcgc
     3721 gtctacgccg gttttcaggt tcagcgccag cccggcttgc atgccatgcg cgcgcagcct
     3781 gcccaggaac gtggcgggat acgccaaggc ctccaggtgc gcgcacaggc ttttcacgcc
     3841 gcaggcgtgc atgccgtcaa ggtaatcgaa tgggttggtg accatcaggt gcgcgtccag
     3901 ttcgcctttc cacacgccgg caatcgcccg caccatgtcc atgccaaagg tcatgctggg
     3961 caaaaagttg ccgtcctcca tatccagatg cagacgcggc agcgattgaa tgcgttgcag
     4021 ctcgctgccg atttccagca aattcccaga ggcgatggat ggaataaccc tcatgcgtca
     4081 cttccttatt aactattccg cgccgccgtg gaagcattcc agggcagcca gcgctttttc
     4141 gtagagcata aattttcgcg cgtaaatctc agcctgctct tcacacggca tcaagcgctc
     4201 gcgtatcgat accatttttg cggcggcatc ctggtaatcc gcgaaagcgc cgcaggcaat
     4261 ccccgcgcag atggccgctc ccttcgcgcc aagctccgag ccctcgggga tttccaccgg
     4321 cagcctcagc gcgtcgcaca tcatttgcgc ccagaccctg gagttggtca ccccaccgga
     4381 aaggcgggct ttgctgaacg cgtgtccgtc tttaatcagc cgacgcacat gaaaaaccgt
     4441 cgaaaacacc acgccttcgt aaaccgcgcg gatgaggtgt ccctgcttat agaaactgct
     4501 taggttaaag aacgcccctt tccctcccga aactgtggca gagtcgaata ggtagggcac
     4561 aaaaaccgcg ctgctctcct caggcgggag cgcctccacg ctttggtcgc agtgttcgta
     4621 aagctgccgc ctggtcttgc cgctgttttc cgccgccaga aagttctcca aaaaccactc
     4681 aaaattgctg gcggaggtcg gcgtggattc ttccaccagg aaatgcccgg gtaaaaagga
     4741 atgcgagacg gagttggtgg tctgcttgta cccgcggcgc agctcaggcg ccaggtattc
     4801 gttgatggac caggtgccgg caatcaggca aagcgtttcg ccgtccaaga ccccgctggc
     4861 aagcgcgccg gcatccacgt caaagcagcc acctgctacg ggcgtccctt ccgccaggcc
     4921 ggtttctgcc gcggcttgcg cgttgacgta cccggaaatc gcaacggggc tgaggcaggg
     4981 cggcatcagg cggaagcaat cttccaggcc caaaagcgcg aacagcccgg gttcgaactt
     5041 gagcgtgtgg atgttcatca atccccagga agaggcttcg gtgaattcgc aagcggctgc
     5101 tccggtcagg tgcaggcgca cgaaatcttt aatccctagc gcccaggcgc tgcgttccaa
     5161 tacctccggc gcgtgctcct taaaccacag gagcagcacc gccggctgcg cggcccaaaa
     5221 accctgatgc gagtacgcga aagcctcgcg ttcgatgccg ctggccgcga acttttcggc
     5281 gtacaagctg ccgcgcgtgt cggtggagac aatacaattc caggtgggtt tcccatctgc
     5341 atccaccagg cacatgccgt ttccgtaacc ggtgagcccg atgccgatga tttcccgcgg
     5401 gttcacgccg ctgctttgaa ggacctcgcg gatgacttcc acgttggtcc gccagacgag
     5461 cccgggatcg cgttcggtca tgccgggctg cggaataatc aggggcgcct ggcgggagga
     5521 aaccgcaatt tcatgtccct gcaggtcaaa caaggcgcat tttattttgg agcccccgtt
     5581 atcgatgccg agaatggttt gcatagccgc ctttttctgg gtgccgcccg agaccagcgg
     5641 ttcctctgaa ttcttacagg aacatttcga ccacttcgcc gatggcgttg cggttgttgc
     5701 tcctgcgggt gatgtaatcc gccgcggctt tggcgtcctc gctggcattg cgcacagcga
     5761 tgccgatgcc cgactttttg atcatctcca cgtcgttgga gaagttgcct accgcgataa
     5821 ccccttcggt cggaattctc aagcgttggg caaggaaact gaccgcgtgc catttgttaa
     5881 tcccgtgctg cagcacatcg taataatgcg gctgggagtg catggtatac aggttcaacc
     5941 cccggcgttc aatcaaccgg tccatagccg cccgcgaagg ctgcccgtag acgcagaagc
     6001 tgtcaatctc agccagcggg gcgtcttccg gcttgcggat cagcaccggt tggtagttgg
     6061 cataacgggc aaaagcttcc acgctcgcat ccatcagcgt cgcgccccaa aagcggccgg
     6121 ctttgatgtg cagcaggcgg ttcttgcgga taaagttctc cacgacgatc tccgcttcgc
     6181 gcgggtccag gcactcatgc aagagcagtt tgcccgtggc cagctcggtg attttcgcgc
     6241 cggtatccac gacaaggtag tccgcgtacg gaaagcgcgc ctcgagcgaa agcacttcaa
     6301 attcgcttct gccggtcaca aagcaaatcg cggccccttt ttcgcgcgcg agctgcaggc
     6361 tgcgcgcggt ggcgtcgctg atttgcccat ggtcatccac ggtagtgccg tccaggtcca
     6421 gcgctaaaag cttcagaggc gtgccaggct tatcggtcac tttagttgag cgtctcccgg
     6481 aaagcgtgga ctggtttcat gaaagccttc acgcgttcgg ggtcaaccgg attgaagagc
     6541 ttgccgtcca ccttgaaggt ggtcgccacc accgcgccgt cgcaaatctt aagcttatcc
     6601 gtgatggtgt ccttgttgca acctgtgttc gcgaagacca cggtttgagg tacggactcc
     6661 ttcacctgcg ccagcagctc gttctccacg ccttttccag ctgccgagcc tgagatgcac
     6721 agcgcgtccg gcgaggcttt gaagaccagc gagcgtgcga ttgccgtcag cgtgcgcgtg
     6781 tccaggttgg tgtcggattc agggttgagg aagtagagca gcttgagttt gtccaggcgt
     6841 aaggcgctct tccgtcgcag cagctcagag agttcattgt tgtaaaaacc gccgtctcca
     6901 acataaactc cggagaaggt gccgcgggca aaggacgcgt ccactgctgc tgaaagctct
     6961 atggtcgcca gcccgtcgga aatgcagtcc acgccgtaag ggatgcggat ttcgtgtatc
     7021 agctcgccaa tcacgcgcgc catcgcgctg ggggtcacaa agctcatatg ccgctggtaa
     7081 ggcaagctga attcgttcga gaaaatgagc ccgtccacgc cgccgtcctg aatggcgtgc
     7141 aggtccgcgc gcgccagctc aatcacgcgg cgcatgctgg cgctttcctt atataagggg
     7201 tctcccggca ggggctgcag atgcagcatg gaaataatcg gtttcttgac atggaatact
     7261 tcttcggtcc agttcatttc agtcctctat ctaataatta ctgcgttctt acgaaaacca
     7321 agcacagcat attgtcatta ccaacctcta atcggatggg acatgggatg atccgcggtg
     7381 ttgcggtcgc aaatcaaggt cacatccgga atgtacttgg gcagcagcgt caccggatac
     7441 tccagggtag gctcgctgaa gagcgccacg cgcaccacgg tctgtttcca ggcgccggtg
     7501 gcaatcatca ggatcgcgcg tttggcggtg agcatggctt tgaagcccag ggttactgcc
     7561 cggggaggga cgcggtcgta gcaagcccca aagctgcgct ggctgagcgc cacaatggtg
     7621 tcgtcattca ggtttacaat gcgggtcttg gagcgggcaa actcttccag ggtgatgcgg
     7681 tgggtatacg gacgcggtgc ctcgcagaaa gcaaccagac ctttggcgcc gacgccggcc
     7741 cagagcgtat ccacgccgcc catctcttcc acaagattgt caatggcatc caggtcgttg
     7801 atgcgcggcc aatggcgctg ttccaccggg acgttcaggt ccgggtctat tctgccgtag
     7861 aaggccgcgt tcatggtgcc ttccagactc tcgtaagtat ccgccacggg atacgggcgc
     7921 ccttcccaat ccaggaaatc atccatatgg aagacatgca aattgcgcat actgattcgt
     7981 tcgcggttaa cgcgctcgat aagtacgtcg tactcatccg tcgggccagc cggcagcacc
     8041 catttggtga gcttgcccag gcggttgttt tccaggattt catcagccat catgttaccg
     8101 atgaggcgca tagcggcagg cctgtcctct ttgaggatca gattgacctt gcgctccgaa
     8161 tgatcctgca gctgctcaag cggcaggctg caccagcggt agagttcttc gcgagtgata
     8221 tccatgctta tgcctcttct tccagcagcg ctttgataac attgtaaccg cgcgcggtgg
     8281 cgtattcagg ttcagtctga taacgggtga acagttcgag cgagaggtag ccgctgtatc
     8341 cgacttcttt cagggtgcgc acaataccgg gaaaatccat ctgcccatcg ccaggaatga
     8401 ggtggtcctc gctgtctatc gctgcatcga tgaaatgaat gtggcgaata tcgctgccgc
     8461 attttttgaa gtattcaacc atgggctccc gggaaagcag cgggcaagcc aggtccagca
     8521 tgcctttcac catcggcgag ttgaccgcct ggatagccca atacaggtca tctgaagtca
     8581 ccaggatggt gccttcattg ggggtgaccg gttccagaat gatcgtctgg ccgatttttt
     8641 cggcatgctc cgccagaact ttcatgtttt cgatgaagag ccggcggacc tctgactcaa
     8701 gccggccgta tccgggatgg ttgcaggcaa acatgcagta ttcggagccg attagtttgg
     8761 cggcatccag cgtacgcttg aagtactcga ggctatcgcg gttcaattcc ggatcttcga
     8821 agaccaagct gtaaggggag ccggtgtttt cgggggcata actgacaatc ggcagttggt
     8881 aacgctcgga aaggtcgcgg attttcctgc cgccgtcgag caggatatcc agtgcgtacg
     8941 cgtgcgggcg gaaaccgccg atttcaatgg cgtcatagtg gtacttggca gccagctcaa
     9001 aagacttttc gagcggatag cgggaaatcc ccgcggtaaa taaactgatt ttcatgtaga
     9061 ctcctttttt tgctgcacgc ttgatccggg tacattttct ccggaaaaaa acccccagaa
     9121 aaaaaactga agggtgaaat ctcgcatttc ccaaataaac agataactta ctacctttat
     9181 gataccataa tttcggaaaa atcaatagat aagctattga caatattata ccaagaggtg
     9241 tagaatacaa agcatgctgg cagaaactct taagctaccc acgcttacgc tcgggatgtc
     9301 catgaaatga actctgaaaa attggttgtg accattcctt ccattttcat ctcaccacct
     9361 tgcgcaagaa ttcgtaccag ctctgcacat tcccttctta tggattgtca ttacggcagt
     9421 caagtacatc ggttagccgt aatttttatc acatcgctca tatgaaaaga aaggatctag
     9481 ttaatggcaa cgcaatctga aacacaagtt tcaccagctg tcggagcaga aggcaatgtg
     9541 ctcggccgca aactgggctt cctcgcagtc ctcgccatcg gcgtcggtac tacgattggc
     9601 tccggtattt tctcctctgt cggcgaagtc gccaacgctg gcggcagcgc gctgatgacc
     9661 gtgctggcgt tcctcgtcgg cggtatcatc atgatccccc agattctcgc ctatgcagag
     9721 ctctcctccg cgtatcccga gaacggcgga gcatatatct atttccgtga agcaggctct
     9781 gatttcctgg ctttcctctc cggctggatc ggcttctggg gcacagacac tcccggcatt
     9841 tcaattatgg ccctcgccat cgccaactat attggcttcc ttaccggaat cgaaggtatc
     9901 tggctgaagc tgatcgctat cgctttcatt gtcttctatg cctggaccca catccgcagc
     9961 gttgaaggcg gctccaagac ccagaccgct ctgaccgtta tcaaaattat ccccttcgtg
    10021 ctgttgatcg ctgttggtct cttctacctg cagggcagcc tgctctccac ctccccagtt
    10081 gctggcgcgc caaccggcat cctggccctc gtagcggcaa tttctgctac cacctggtcc
    10141 tatgacggta tgcagtccgc tgcctgggtt gccggcgaag tcaagaatcc ccgcaagaac
    10201 atgcccaagg ctctgatctc cacggtgctg ttcgttaccc tgatgtacac cctgatctcc
    10261 ctggtcatca ccggtttgct gcccttcgac caactgacca cttccaccgc acctgtcgcg
    10321 gatgccatgg ctcagatccc cttgatcggg caggctgccg gtatcattac cgcaatcatt
    10381 gccatcctgg tcatcaacgg ctccctgacc agcctcatcc tgatccaacc ccgtcagcag
    10441 tgggctatgg ctcaggacgg catgtggttc cgctccttcg gcaaggttca tcccaagtgg
    10501 cagacccccg tgctctccat tgttgtgcag tgcgccttcg gtattctgtt cctgttcttt
    10561 gcctctctgc gcgacctgct cggatacttc accttcgcgc tgcttttgcg caacaccgcc
    10621 actatggcct ccatcttctg gctccgcaag aaagaaaact acaagcccag ctacaagatg
    10681 cccgcgtggc agctgatgac gatcctggct attgctttct ccttcatcct gttggtctcc
    10741 accctcctgt gggcacccgg tcccagcctg atcgccggcg tcatcggcgc agtcactggc
    10801 tatcctgcct acctgtactt caagaagacc cagaaaccgt tcgaaacctt tggtaacgag
    10861 gaagcttagt cttcgaacta aaccgaactg tcagtaaacc gatgtaattc tcgggggcat
    10921 gggaagcggc cgcgcagaac atgccccctt aattttgaaa aaacaaacaa aagaaagaac
    10981 atatggctca ttattttcaa aaccttgtca cagaactgat taccccattc aacgatgacg
    11041 gcagcgtgga ccacgagacc ctcgccgcga ttgttgactt ccaaattaaa aatggcgtac
    11101 ggcacttctt cgtgaacggc ctcggcggcg aaagccacct gctcagcttc caggaacgtc
    11161 aggatgtgct gcgcacggtc tcctcgcact gcaccgacgg cgccaaaacc atggcgtgcg
    11221 tgtttgtctc caccgtgcgg gaaggcaaag aactgatcga catgtacgcc gacgtccaac
    11281 acgacgcagt ctgccttacc gctccggcat acttccctta caccgagcaa gcgctgtatg
    11341 ccttcacttc ggagctgctg gaatacgcca ggcagcccgc ctacatctac aactgcgtcc
    11401 agatgggcac gctgtactgc cccgagctgc tcgcgcgtct ggcaaaagac cacagcaacc
    11461 tgtacggtta caaaaatgcc acgcgcgata ttgtgcacta catgcagtgc ctgatgcgca
    11521 ttgacgcgac caagttcgac tttttggacg gctgcgacgc gacaatcgcc cccactatgc
    11581 tggctggcgg cgtcgggtgc gtatcgttca tgggcgtccc cttccccaag gaaaccaaag
    11641 ccatctgtga ttacgcgctg gccggcaatg ccgaaaaagc catgcaggct caattcagaa
    11701 ttctggaact gcgcaacatc ctcaagaagt ccccgttcaa cgccgcgtac atgctggcga
    11761 tggaactctc gggcggccct gttgcccgca acacgcgcat ggtgcccgag atgaccttgg
    11821 tatccgatgc ggttcgcaaa gagctgttcg agacttacgc gcgccagcaa gcagagccga
    11881 tggcataagc gcacttgggc ttagcttcta cccgcgcgac tgaaagattg agatgatgaa
    11941 cagcaccaaa attgaattga atgaagcagc attctctgaa aaggaaaaac aatttctcca
    12001 aaatgaggca ttcaccgccg tcctgttccg ctattcgacg caggtctgcg cggtacggct
    12061 cagcaattcc aaaatcaatc tggtggtgct gcctttccaa gggcaacaag tctggcgttt
    12121 cgcaagtcca atcggtaaca tgacgatgaa gtccatcttt gatgagccgg aaaacaccac
    12181 ccgcttcggg ctgaactacg gcgccttcct gatccactgc ggattgaccg ctaacggcaa
    12241 cccctccgcg gaagattcgc accccctgca cggcgagctg cccaactgcc attaccagga
    12301 agcctatttg cgcgtcggga cggatgagaa aggtgatttt ctcgctctgg gcggcagcta
    12361 cgtctaccgc aacagcctgg aataccatta tgaattcgcg ccggaactgc gcatgcgtcc
    12421 ggattcagca ctggtggatg tgcacattca ggcgcgcaac ttccgcccta aagcgatgaa
    12481 gtacatgtac atggcgcata tcaattggct gcctgtggac tgctcccgcc tggttgccag
    12541 cgcgaacccg aaggccaccg aggtattctc cgatactttc gggctgccca acagcgacca
    12601 acagaagttt atggattaca tagagcgcct tggcgcagac cccacgatcg cggataaaat
    12661 agactcatcc agccaaattt acgacccgga agtgtgcatg tatctgcatt acagcgcgga
    12721 cgaacaggga tacgcgcacg cgatgcaggt tttccccggc ggagacgcag cctacgtcgc
    12781 tttccgtacc aaggagctgc cgaacgcggt gcgttggttc gcacgcacgg gcagcgaaga
    12841 tgcgcttgcg tttgccatcc ccagcacgaa caaccacctg gggttcgcgc gcaacgacgc
    12901 caaaggtctt atccgggaaa ttggagccaa agattgtctg gaaatgcatt atcggttcgg
    12961 ctatctgagc tgcgatgaac gtcgtgatgt tgaaacgaaa attgataagg ttctcggcaa
    13021 ggcttaatcc cgcctgatga gccgcgcgag cagaaggagt ttgaaatgac aaaagaaatc
    13081 gatgtgattg ttctaaacag ccacggcgtg ggtcagttct gtaatatcaa acgtctgcct
    13141 tttttgggcg agactctgcg cgcctggaac tggcgcgtgg aagaagacgg gggcaaaggc
    13201 accacagtgg ctgtttcgct cggccgcctg ggcgtaaaaa ccgcctatat cggcaaggtc
    13261 ggcaaagacc cgtggggcga cctgggtttc aaatggatgg gcgacgcagg cgtggatacc
    13321 acctacatgt accaatccga tgaggtcgca acgggcaccg gcctggtgat gattgacgaa
    13381 gacggcaaca acaccatcgt ggacggtgac agctcgcttg aaaaactaac cgttgaagaa
    13441 atccgcaccg cgttggaaga catgaaaaac gccaaattct tcatcaccgg tttcgccatg
    13501 ccgcagcatc tggcgctggc cagtgcgaag atggcccacg aaatgggcat tgccaccgtg
    13561 ctgaacccca gcccgctccc ccacgaaccg atgggcgacc tgagctacat cgattacatc
    13621 gtgtgcaacg aagtcgaagg ccgcgttctg gccgagtttg aggaaggctc caccgaatac
    13681 gagaaaatcg cgcgccgtgt gcgtgagatt taccactgcg gttcaatcgc aatgaccctc
    13741 ggcgaagagg gctgcctggt gctgcacgag gaccaaattt accgcgtgcc ttcagtcaag
    13801 gtggatgtgg tggagactgt tggcgcgggg gacagcttcc tggcagcctt tgttgccaac
    13861 ctggtatggg gcaaatctgt gcaagaggcc gcggcttggg cggtcaaatg ctccgcgctc
    13921 aaggtcacgc gggccggcac catccccgcc ttcccctacc tccgtgaggt ggaggccttc
    13981 attgctaacc tggatgagag caagtgaggc gtgcccgcgt ttgtgatttc tggtcgacag
    14041 acaaaaggac cggttcgaac gaaccggtcc tttgttatat attgctttgt gtcagatgtt
    14101 tttctgtgaa ctgaggatta tgttaatggc atacccagat tcgtcagtgt ga
//
LOCUS       DAOQAD010000043        14058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_4725_length_14058_cov_5.819110, whole genome shotgun sequence.
ACCESSION   DAOQAD010000043 DAOQAD010000000
VERSION     DAOQAD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14058
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4725_length_14058_cov_5.819110"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1842
                     /locus_tag="PKL60_04910"
     CDS             256..1842
                     /locus_tag="PKL60_04910"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNW95511.1"
                     /translation="MREIPSPNTAAPDKKPAAYLTTTVFLAGAVTMAVEFGASRLLGN
                     VFGTSNIVWAVVIGLVLVYLTLGSWLGGRLADKNPSARVYYSVLAWAALGAGLVPLLS
                     RPVLRAAAEAFDALNLGVLAGAFITVLLLFSIPVILLGMVTPFALKLLIPSTDKTGRI
                     SGRLSAISTLGSFLGTFLTVLALIPWVGTYRTFLLVSMSLLLAAILGLWSLRARRQSL
                     LFLLLGVLLLVFSVLGLRGSDKRTPGLVYETESAYNYIQVLEVGGFRFLRLNEGQGVH
                     SIYHPTEYFFSGPWDQVMAAPFFNPSAAPAEINRIALLGLAAGTSARQASVVYPHARV
                     DGFEIDPKIVQVGREWFGMDLPNLTVYTEDARWGLAHSAGEYDIISVDAYRPPYIPAH
                     MVTQEFFALVYEKLADDGVMVINIGRSAQDRSLVDTLSATVAQSFPAVFVTDLPDSYN
                     TILFAAKNPQASWEAFERNQKLLNVPGENAQLTQIMAVTLGGKAAVSFADKIFTDDQA
                     PIEFMTNRLVINFLLEEDKE"
     gene            1845..2516
                     /locus_tag="PKL60_04915"
     CDS             1845..2516
                     /locus_tag="PKL60_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HNW95512.1"
                     /translation="MKSTLDKILGALLAVMLPFILLLGAVRLIMTPQFPALEYGRPGF
                     PEDSFGFTNAERKQWSAYAVNYLVNDADIDYLADLRFPDGKALYRAEELSHMEDVKAV
                     VKTALTVWQGLLGAAFALCVWFAAMGAWRKLGKSLRTGAWLSIVLIVFIVVMTAVSFD
                     MLFDRFHRLFFTEGSWLFYQNDTLIRLFPLVFWRDAFLLVGLVVLVAAGLLLWVTRGA
                     KGKTD"
     gene            complement(2586..3695)
                     /gene="ugpC"
                     /locus_tag="PKL60_04920"
     CDS             complement(2586..3695)
                     /gene="ugpC"
                     /locus_tag="PKL60_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNW95513.1"
                     /translation="MASVMYDHVWKKYGDVVAVQDLNLAIEDKEFLVLVGPSGCGKTT
                     ALRCLAGLEEITQGEVKIGDQVVNDVAPKDRDIAMVFQSYALYPHMSVYDNMAFGLKL
                     RKMPKDEIDRRVKESARILGIEGFLKRKPKELSGGQRQRVAVGRAIVREPKVFLFDEP
                     LSNLDAKLRVQTRAEISKLHKNLQTTFIYVTHDQTEAMTMATRIAVMSKAKLQQLDIP
                     QNLYDHPANLFVAGFIGSPAMNFFKGKLVQADGHFVADLGSFSVKLPDQDKERYRSVL
                     DKDIILGIRPEDIHNPLYAPPGIIAEPVDVKVDVTELMGNEIFLYLVNGEHNFIARVD
                     PRTTAHFGDEMQVVFNMDNYHIFDPGLDAENPPVV"
     gene            complement(3811..3972)
                     /locus_tag="PKL60_04925"
     CDS             complement(3811..3972)
                     /locus_tag="PKL60_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNW95514.1"
                     /translation="MDKYECIACGYIYDPEIGDPDNGIAPGTAFEDLPLDWVCPDCGV
                     SKDQFEKIR"
     gene            complement(4118..4468)
                     /locus_tag="PKL60_04930"
     CDS             complement(4118..4468)
                     /locus_tag="PKL60_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNW95515.1"
                     /translation="MEENLSPTPLWDVDKTHPQYHDDLIENLAGITDPELGYSIIELG
                     LVRNISIENDTARVVMILTTPFCPYGPNMLESTRAKVEQVLGLKTELEYGQETWTPAF
                     MEKDLMDDDWGLLP"
     gene            complement(4471..5079)
                     /locus_tag="PKL60_04935"
     CDS             complement(4471..5079)
                     /locus_tag="PKL60_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNW95516.1"
                     /translation="MRSTRDKILEILQTKRKATISDLAKVVGINGISVRHHLINLQEE
                     GLIYAEEERHGVGRPRFVYRLTDQGLEKFPSNYQKFTRYMLTELKNMLTSEQLIALFE
                     KIGKSQAESTLNIDQSLPLEEKLELLADQLSGDGYRISWKKDSTKLFLYSENCPYHNL
                     GIVHAEICRIDEAMFTCALNREVVHTQCICKGDPRCMFVLDL"
     gene            5234..5638
                     /locus_tag="PKL60_04940"
     CDS             5234..5638
                     /locus_tag="PKL60_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNW95517.1"
                     /translation="MANLLDIEGIGPVYAEKLQKVGVKSVEALLKLGATPKEREELAE
                     KAGIDKKLILEWVNRADLFRIKGIAEEYSDLLEAAGVDTVVELANRNAEHLLAKILEV
                     NEVKKLVRRVPTLAQIEKWIEQAKELPRAIHY"
     gene            complement(5737..6567)
                     /locus_tag="PKL60_04945"
     CDS             complement(5737..6567)
                     /locus_tag="PKL60_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95518.1"
                     /translation="MAQALTLPSIFKAVSKTLSENEAALNEADTYNHNHGSNMAKAFT
                     TVSKAVAKKKDLPVSEQLTYASKVLQDSTSSSAKIYAQGLANAAQKFQGKQLNENTVG
                     LLIDSMMGIQEPAQAASGAAEKSLLGSLLGGLAGGQQQQQQTQPSGGDLLGSLLGGLA
                     GGQQQQQTQPSGGDLLGSLLGGLAGGQQQQQTQPSGGDLLGSLLGGQSNPDNGLDITD
                     LLSAGLSYYSAKEQGGSTMEAIMQALASASPLGQRDDQKQSGALVINTILNMLKKKKK
                     "
     gene            complement(6580..7053)
                     /locus_tag="PKL60_04950"
     CDS             complement(6580..7053)
                     /locus_tag="PKL60_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNW95519.1"
                     /translation="MAEEKKSFFKKIGDALSNRDEKEAAAKAAAEAAAKAAAEAKAKA
                     EAEAAKLKAEAEDKKQDLLEAQERYAREAEARKAEYEARKAAEEEARKNAILAIHVVA
                     SGETLSHIALQHYGHATPPYYKLIYEFNKDVIGDNINIIIPGQELRIPVLPEELK"
     gene            7284..8069
                     /gene="sufC"
                     /locus_tag="PKL60_04955"
     CDS             7284..8069
                     /gene="sufC"
                     /locus_tag="PKL60_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNW95520.1"
                     /translation="MTVLEIRNLCVSVNDKEIIRDFNLSMRQGEIHALMGPNGTGKST
                     LAYAIMGHPRYQITGGDVLMDGESILGLSPDERAHKGIFLAFQYPVSIPGVSLANFLR
                     VAVNARNKALNPNDKGISVVKFRQMLKSKMDLLKMDYQFASRYLNEGFSGGEKKRAEI
                     LQMAMLEPSFAILDETDSGLDIDALRIVSEGVNTLAQGHIGVLVITHYQRMLNYISPD
                     FVHIMYGGRIVESGKAELALRLEEQGYDWIREKYGVLPDGVHD"
     gene            8086..9498
                     /gene="sufB"
                     /locus_tag="PKL60_04960"
     CDS             8086..9498
                     /gene="sufB"
                     /locus_tag="PKL60_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNW95521.1"
                     /translation="MADHHQDFLQSLGNADKYGFSYPDDSAFRSRKGLDEDVIRQISA
                     YKEEPEWMLEFRLNAFRHYRSRAIPTWGADLSGLKLDDIIYYARPKQIDQKSWEDVPE
                     NIKNTFDRLGLPEAEQKYLAGVGAQYDSEMVYHNIQENLERQGVIFLSIENGLKQYPD
                     LFREHFASVVPYADNTFAALNSAVWSGGSFVYVPKGVHVDLPLQAYFRLNMANIGQFE
                     RSLIIADEGAQVHYVEGCTAPLYLTESIHSGVIEIIVKKGARVRYTTIQNWSTNVYNL
                     VTQRAVVEEDGTMEWVDANLGSKVTMKYPACFLKGPGAHGEVLSVAFAGPGQHQDAGA
                     KMIHFAPNTSSKITSKSISRGNGRSSYRGLVRVAAAAENSRSSVVCDALLLDHESRSD
                     TYPTMDIKARDVQIGHEATVSKIGEEQLFYLNSRGLSDEEATTMVVSGFIEPLVKELP
                     MEYAVEMNKLIQLQMTGSIG"
     gene            9507..10841
                     /gene="sufD"
                     /locus_tag="PKL60_04965"
     CDS             9507..10841
                     /gene="sufD"
                     /locus_tag="PKL60_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNW95522.1"
                     /translation="MRTNLLEFLNSNRPELPVVVAGRQPLGAAPKLLEFRQAAWRHAQ
                     SLPFPGKKDEAWRRVNLDDLLNTPLNMLSAKKQKLRINAGIPAREAAGYAGALFSSPN
                     GSSLSLDPALEQAGVLFCDFTEAQRTHAEFLVDHLGEAVPPGDGKFAAAASALAEFGA
                     VLNVPKGFRVEKPLLVRLEASQAGSAAFSHNLIRLESGASAVLVLDFNSPAAAEGSLQ
                     ALHLGILELQLEANAELTLVELQQFSRQTWNITHERAVLGEGARLHWIYGALGGSLSK
                     NFLETSLQGAGAEAKMHGFYFASGRQVFDLDTQQDHLAPHTTSDLLYKGAAAGNARTV
                     WEGMIFVAPQAMQTDGFQTNRNLVLSEGAEVNALPGLEILADDVRCSHGASIGRLDED
                     ELFYLQTRGIPRREAEQLVMEGFFGEIVERVPVASLQSELKKLLSARFNQAV"
     gene            10888..11169
                     /locus_tag="PKL60_04970"
     CDS             10888..11169
                     /locus_tag="PKL60_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95523.1"
                     /translation="MRSPEPEITYEVGDKVEVNCDHEKNGQRVRGWLRGTVVQVDDKN
                     LAVQFRTNIYLTDGWMVPDQILWFPFDSANVRRIQPKAKSSGKPDFNLL"
     gene            complement(11173..11820)
                     /locus_tag="PKL60_04975"
     CDS             complement(11173..11820)
                     /locus_tag="PKL60_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNW95524.1"
                     /translation="MNNMTFFGKSVLFLGAHPDDIELGCGALLADIVGQTELYCMTFS
                     DNKKNPDLQHLLDEHYVSMRTLGLRDDQIEVGSFETRRFPDFRQEILEKMLKLRRQLQ
                     PQIVFVHTAQDIHQDHVTLTQEALRAFRGTTVLGYDVLRSSYGFFPHFLVEVTEAGVN
                     KKIEALSRYTTYAERYYFSEAVLRSTAVRHGALAERPFAEGFDIIRIVGSFGPAA"
     gene            complement(11829..12629)
                     /locus_tag="PKL60_04980"
     CDS             complement(11829..12629)
                     /locus_tag="PKL60_04980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW95525.1"
                     /translation="MKIDFQETTNDLLKRIDIHSQYGARDIDSWMLDILQLKKGLRIL
                     DVGCGAGKQCFSFHQHLDGEAEITGTDVSESLLAQARQENEKRGANVRFQLLDFNQAF
                     DLPGDSFDLISACFAIYYAQDIPFTLAEMHRVLKPGGRLFTTGPMPTNKAIFYDIIKE
                     ATGKAIPPMPGSSRYASDIYGTMEKLFSKVEQHIFENPLTFTDAEPFMVYTRASMSED
                     RKLWNSLFITHDDFEIVMAQIEKVARARIAKDGSLVMTKVVGGFIATK"
     gene            complement(13163..>14058)
                     /gene="hypE"
                     /locus_tag="PKL60_04985"
     CDS             complement(13163..>14058)
                     /gene="hypE"
                     /locus_tag="PKL60_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNW95526.1"
                     /translation="GELTPPPGFQLVVSTDAHVVFPLFFPGGDIGRLSVCGTVNDVAM
                     LGAQPKYLTASFILEEGLSIPELEAVLASMREACTEADVRIIAADTKVTEKGKADKLF
                     ITTAGFGYAPAGRRITGDQAQPGDAVLISGALGNHGIAVLQARGNLGFQSDVRSDAAP
                     LNHLTQGLCAAFPDLHVMRDPTRGGLATTLVEIAGQSGVTIQLNEPDIPIDQPVRAAC
                     DLLGLDPLYLANEGKVIVILPEAQAESALNYLRTQKYGSGARRIGSVVSRSSGQLLLR
                     TAFGTTRILDMLAGEMLPRIC"
BASE COUNT         2986 a         3660 c         4082 g         3330 t
ORIGIN      
        1 agctgcatga taggtagcgc tgatgttgcc ccgtcgtagg tcgggtggag cggcagcaca
       61 acccgacaag ctttgcccag cgggtttgtg tagccgctgt tgggtttcgc tcccgcgcct
      121 ctgcacccag ctaaaacgga ttgtcatcct gaacgcggtg gagcagctct ggcgtgtagc
      181 gattcttcat ttgctcctca gaatgacagg aaaaccgctc aaaccaccct cccgaaaccc
      241 cgctataatt ggggcatgcg tgaaatccca agcccaaaca ccgcagcccc cgataaaaag
      301 cccgctgctt accttaccac gacggtcttc ctggccggcg cggtcaccat ggcggtggag
      361 ttcggcgcgt cgcgcctgct gggcaacgtg tttggcacca gcaacatcgt ctgggcggtg
      421 gtcatcggcc tggtgctggt ctacctcacg ctcggcagct ggctgggcgg ccggctcgcc
      481 gacaaaaacc cctccgcgcg cgtctattac agcgtgcttg cctgggcggc gttgggggcg
      541 gggctggtgc cgctgctctc gcgcccggtg ctgcgcgccg ccgccgaggc ttttgacgcg
      601 ctcaatctgg gtgtgcttgc cggtgcgttc attaccgtcc tcctgctttt ctccatcccg
      661 gtcatccttt tggggatggt gacgcccttc gcgctcaagc tgctgatccc gagcacggac
      721 aagaccggca gaatctccgg caggctctcc gcgatttcca cattaggttc ctttctgggc
      781 acctttctga cggttctggc gctcatcccc tgggtcggca cctaccgcac tttcctgctg
      841 gtgagcatga gcttgcttct ggctgccatc ctgggtttgt ggtccttgcg cgcacggcgg
      901 cagagcctgc ttttcctgct gctgggtgtg ctgcttctgg tgttttctgt tctgggttta
      961 cgtgggagcg acaaacgcac acccgggctg gtgtatgaaa cggagtcggc ttacaactat
     1021 attcaggtgc tggaagtggg cggcttccgc ttcctgcgcc tgaacgaagg ccagggagtg
     1081 cactcgattt atcacccgac cgagtacttt ttctcaggcc cctgggacca ggtgatggcc
     1141 gcgcccttct ttaatccctc cgctgcccca gcggaaatca accgcatcgc gctgctgggc
     1201 ttggcggccg gcaccagcgc gcgtcaggcc agtgtggtct acccgcacgc gcgggtggac
     1261 ggctttgaga ttgacccgaa aatcgtgcag gtcgggcgtg agtggtttgg gatggacctt
     1321 cccaatctga ccgtctacac cgaagacgcg cgttgggggc tggcgcattc cgccggtgag
     1381 tacgatatca tcagcgtgga cgcttaccgc ccgccataca tccccgcgca tatggtcacg
     1441 caggagtttt tcgcgctggt gtatgagaaa ctcgcagatg acggcgtgat ggtcatcaat
     1501 atcggcagga gcgcgcaaga ccgttcgctg gttgatacgc tcagcgcgac ggtcgcgcag
     1561 agcttcccgg cggtttttgt caccgacctg ccagactcgt ataataccat cctgtttgcc
     1621 gctaaaaatc cgcaggcatc ctgggaagct tttgagcgca accaaaaact gctaaacgtg
     1681 cccggggaga acgcgcagct cacgcaaata atggctgtca cgcttggggg caaggcggcg
     1741 gtaagcttcg cggataagat tttcaccgat gatcaagcgc cgattgagtt catgaccaac
     1801 cggctggtaa tcaacttcct gctggaagag gataaggagt gagtatgaaa tcaactttgg
     1861 ataagattct cggcgcgctg ctggctgtga tgctcccgtt catcctgctc ttgggggcgg
     1921 tccgcctgat aatgaccccg caattcccgg cgctggaata cggacggccc ggatttccgg
     1981 aagactcttt cggtttcacg aacgcggagc gcaagcagtg gtccgcgtac gcggtcaatt
     2041 acctggtgaa tgacgctgat attgactacc tggcggattt gcgcttcccg gatgggaagg
     2101 cgctttaccg ggcggaagag ctcagccata tggaggacgt caaggcggtg gtcaaaactg
     2161 ctctgactgt ctggcagggt ttgcttgggg cggcgttcgc gctgtgtgtg tggtttgcag
     2221 cgatgggcgc ctggcgcaag ctgggaaagt ccctgcggac tggggcctgg ctttccatcg
     2281 ttttgattgt ctttatcgtg gtgatgacgg cggtcagttt tgacatgctc ttcgaccgct
     2341 ttcatcgtct gtttttcacc gaaggctcct ggctttttta tcagaatgac acactcatcc
     2401 gcctgtttcc gctggttttc tggcgtgatg cttttctact ggttgggctg gttgtacttg
     2461 ttgcggcagg gctcctgtta tgggtgaccc gcggcgcaaa aggaaaaaca gattgaacct
     2521 ctctgatcat gaaacagaaa aggctgcccg taaaggcagc ctttggttat tgagggattt
     2581 atagcttaga caaccggagg attctcggcg tccaggcctg ggtcaaagat gtggtagttg
     2641 tccatattga agacaacctg catctcatcg ccaaagtggg cagtcgtgcg cgggtcaacg
     2701 cgggcgataa agttatgctc gccattgacg aggtacagga agatttcgtt acccatcagc
     2761 tcggtgacgt ccactttcac atctacgggt tcggcaatga tgccgggcgg ggcgtagagc
     2821 gggttgtgga tatcttccgg gcggatacct aaaatgatgt ctttatcgag gacgctgcga
     2881 tagcgttcct tgtcctgatc cggaagtttg acgctgaagg aacccaaatc ggcgacgaag
     2941 tgtccatcag cctgtaccag cttgcccttg aagaagttca tcgcggggga accgatgaag
     3001 ccggccacga acaggttggc ggggtggtcg tagaggttct gggggatatc caactgctgc
     3061 agtttagctt tgctcatgac ggcgatgcgg gttgccatgg tcatggcttc ggtttggtcg
     3121 tgcgtcacat agataaaggt ggtctgcagg ttcttgtgca gcttgctgat ttcagcacgg
     3181 gtctggacgc gcagcttggc atcgaggttg gaaagcggct cgtcgaagag gaaaactttc
     3241 ggttcgcgca cgatggcacg gcctactgct acgcgctggc gctgaccgcc ggagagttcc
     3301 tttggtttgc gcttgaggaa gccttcaatg ccgaggatgc gggcggattc ttttacgcgg
     3361 cggtcgattt cgtccttggg catcttgcgc agcttcaggc cgaaggccat attgtcatac
     3421 acagacatgt gcggatagag cgcatagctt tggaaaacca tggcaatatc gcggtctttt
     3481 ggagcgacgt cattgacgac ctggtcgcca atttttactt caccctgggt aatttcttcc
     3541 aaaccggcca gacagcgcag cgcagtcgtc ttgccgcagc cggatggacc aacgagaacg
     3601 aggaattcct tatcctcgat tgcaagattg agatcttgca cagccacaac atcgccgtac
     3661 tttttccaaa catgatcgta cattacactt gccataattt tcctccattc tgtgcggata
     3721 tagtgacttc attataccct ttatttgtca agggaattaa acaaaaagcc ttattccacg
     3781 gaataaggct ttttgttgtg ataagtagga ttatctgatt ttttcaaact gatctttaga
     3841 aacgccgcag tcggggcaca cccaatcaag cggcaggtct tcaaaggcgg ttccgggagc
     3901 gatgccgttg tccggatcac caatttcggg gtcgtaaatg tacccgcaag cgatgcattc
     3961 gtatttgtcc atttttctcc ttcttcatta ctccagttgg accaggtcca ggaaattctt
     4021 cgccggcagt ttgctgactg cgcggtgaat taattataca ataagtttcc tatttggcca
     4081 tgtgaattgc agcgtttctt ctggctgctt cgagtcatta cggcagcaga ccccagtcgt
     4141 cgtccatcag gtctttctcc atgaatgccg gggtccaggt ttcctgtccg tactcgagtt
     4201 cggtcttcag gcccagcacc tgctccactt tcgcgcgggt ggattccagc atgttcggtc
     4261 cgtatgggca gaaaggggtc gtaaggatca tcaccacgcg ggccgtgtcg ttttcgatgc
     4321 tgatattgcg caccaggccg agctcaatga tggaataacc caattcaggg tcagtgatgc
     4381 cggcaaggtt ctcgatcagg tcgtcatgat actgcgggtg ggtcttgtct acgtcccata
     4441 gaggggtggg agagaggttt tcttccatgt ttataaatcc aatacaaaca tgcagcgcgg
     4501 atcgcccttg cagatgcatt gagtatgaac gacttcacgg ttgagcgcgc aggtgaacat
     4561 tgcttcgtcg atcctgcaga tttcggcgtg gactatgccg aggttatgat acgggcaatt
     4621 ctccgaatag agaaaaagct tggtgctgtc cttcttccag ctgatgcgat agccatcacc
     4681 ggaaagttga tccgcgagca gctccagctt ttcctcgagg ggcaggcttt ggtcaatgtt
     4741 cagcgtgctt tctgcctgac ttttgccgat tttttcgaac aacgcaatca gctgctcgct
     4801 ggttagcata tttttaagct ctgtgagcat gtatcgggta aatttctggt agttgctcgg
     4861 gaatttttcc aacccctggt cggtcaacct gtatacaaag cgaggacgcc cgacgccgtg
     4921 gcgttcttcc tccgcgtaga tcaagccttc ctcctgaaga ttgatgagat ggtgccgcac
     4981 cgagataccg ttaatcccga ctactttagc gaggtcgcta atagtggctt ttcttttcgt
     5041 ctgaagaatc tcaagtattt tgtcacgtgt acttctcata tttgctttcc aaaccttcgc
     5101 tccctgcagc gaatttctcc ggggctgcgt aaataattaa tattctttat agtaattttc
     5161 aagaatttta ctatactttg gtatcgttgt gttataattt aacaaattga gatgaaaata
     5221 gaggagatga atcatggcaa acttgttgga tattgaaggc atcggacctg tatacgccga
     5281 aaagctgcaa aaagtcggcg tcaagagcgt ggaagcactg ctcaaacttg gcgcaacccc
     5341 taaggagcgc gaggagcttg cagaaaaagc tggcattgac aagaagctga tcctggaatg
     5401 ggttaatcgc gccgacttgt tccgcatcaa gggcatcgcg gaggaatatt ccgacctgtt
     5461 ggaagcagca ggcgtggata ccgtggtcga gcttgccaat cgcaacgccg aacacttgct
     5521 ggcgaagatt ctggaagtta atgaagtcaa gaagcttgtc cgccgggttc ccaccctcgc
     5581 tcagattgaa aaatggattg aacaggcaaa ggaactgccg cgcgccatcc actactaaac
     5641 cagcgcttct gagcaagcaa ccagagcgcc cgaaagcaga taaagagata aaaaaaccgc
     5701 ccgatttggg cggttttctt tggtgtacag tcagccttat ttcttcttct ttttcaacat
     5761 gttcaggatc gtgttaatca ccaacgcacc ggactgcttt tggtcatcgc gttggcccag
     5821 cgggctggca gaagcaagcg cctgcatgat ggcttccatg gtggagccgc cctgctcttt
     5881 ggctgagtag tacgacaggc ctgcggaaag caggtctgtg atgtcgagcc cgttatccgg
     5941 gttggattgc ccgccgagca gcgagccaag caaatccccg cccgaaggct gggtctgctg
     6001 ctgttgttgg ccgccggcaa gcccgccgag cagcgagcca agcaaatccc cgcccgaagg
     6061 ctgggtctgc tgctgttgtt ggccgccggc aagcccgccg agcagcgagc caagcaaatc
     6121 cccgcccgaa ggctgggtct gctgctgctg ttgttggccg ccggcaagcc cgccgagcag
     6181 cgaaccaagc aggcttttct ctgccgctcc gctggctgcc tgggcaggtt cctggattcc
     6241 catcatcgaa tcgattagca aaccgaccgt gttttcgttc agctgtttgc cctggaattt
     6301 ctgggcagca tttgcgagcc cttgcgcgta aatctttgct gaactgctgg tggagtcttg
     6361 cagtaccttg ctggcatagg taagctgttc ggaaaccgga aggtctttct ttttggcaac
     6421 tgccttggaa accgtcgtaa aggctttcgc catgttcgag ccgtggttgt gattgtaggt
     6481 atccgcctca ttgagggcag cctcattttc gctgagagtc ttgctgacgg ctttgaaaat
     6541 ggaggggagt gttagcgcct gtgccatctt ggatatgtcc tatttgagtt cttcaggaag
     6601 tacggggatg cgcagttcct ggccggggat gataatgttg atgttatcgc caatgacatc
     6661 cttattgaat tcataaatca gtttgtaata aggaggggtg gcgtgtccgt agtgctgcag
     6721 cgcgatgtgg ctgagagttt cgccggaggc gacaacgtgg atggccagga ttgcgttctt
     6781 cctggcttct tcttcagcgg ctttgcgggc ttcgtattca gccttacggg cttccgcttc
     6841 gcgggcgtag cgttcctgcg cttcaagcag gtcctgcttc ttgtcttcgg cttctgcctt
     6901 cagtttggcg gcttcagctt ccgctttggc tttggcttcc gcggcagctt tggcggctgc
     6961 ttccgcggcg gctttggcag ctgcttcttt ctcgtcgcgg ttgctcagag catcgccgat
     7021 cttcttgaaa aaacttttct tttcttctgc catgataatc tcctaatctg gctgtaattc
     7081 cgcgaacgcc aaagcggcac atctcgcggt tattaaaaat ccggggaaac tcccctcagc
     7141 gaaagaatta tactccactt ttgatacttt tccacgcatg aataatgtta atatacttaa
     7201 tgtatattat ataaattgac ttttaaacaa cgcaatgata taatcgcgca gactttgcaa
     7261 gctgttcata cctggaagga aaaatgaccg tgctggaaat cagaaacctg tgtgtatctg
     7321 taaacgacaa agagattatc cgggatttta acctgagcat gcgccagggc gagattcacg
     7381 cgctcatggg tccgaatgga acaggaaagt ccacgctcgc ttatgccatt atgggtcatc
     7441 cccggtatca gattacgggt ggggacgtgc tgatggacgg agagagcatc ctggggttaa
     7501 gccccgacga gcgtgcccat aaagggattt tcctggcttt tcaataccct gtatcaattc
     7561 cgggcgtgtc gcttgcgaat ttcctgcgcg tagcggtgaa tgcccgcaac aaagcgctga
     7621 acccgaatga taaaggcatt tcggtggtta agttccggca gatgctcaaa tccaagatgg
     7681 atttgctgaa gatggattac caatttgcca gccgctacct gaacgagggc ttttccggcg
     7741 gagagaaaaa gcgcgcggaa attctgcaga tggcgatgct ggagcccagt ttcgcaattc
     7801 tggacgaaac cgactccggc ctggatattg acgcgctgcg catcgtttcc gaaggggtta
     7861 ataccctggc ccaggggcac atcggcgtgc tggtgatcac gcattatcag cgcatgttga
     7921 attacatcag tccggatttc gtccatatta tgtacggcgg gcgcattgtt gaatccggta
     7981 aggctgagct ggcgctgcgc ctggaagagc agggctatga ttggatccgt gaaaaatacg
     8041 gcgttctgcc tgacggcgtg catgattaaa cgcggataga gcaaaatggc tgatcatcat
     8101 caggactttt tacagtcgct tggcaacgcc gataaatacg gtttcagtta tcccgacgat
     8161 tcggctttta ggagccgcaa gggtttggat gaggacgtta tccggcaaat ctccgcgtat
     8221 aaagaggaac cggaatggat gctggaattc aggctgaacg ctttccggca ttatcgcagc
     8281 cgggcaattc ccacctgggg tgcggatttg agcggtctca agctggacga tatcatttat
     8341 tacgcccgtc cgaagcagat tgaccaaaaa tcctgggaag acgtccctga gaatatcaag
     8401 aataccttcg accgtcttgg ccttccggaa gcggaacaaa agtacctggc gggcgtgggg
     8461 gcgcagtacg actcggaaat ggtctaccac aatatccagg agaatctgga aagacagggc
     8521 gttatcttcc tgagcattga gaacggattg aaacagtatc cagacctgtt tcgcgagcat
     8581 tttgccagcg tggttccgta cgcggataac accttcgccg cgctcaacag cgcggtgtgg
     8641 tcaggcggct ctttcgtcta cgtccccaag ggcgtgcacg tggacctgcc cttgcaggca
     8701 tatttccgct tgaacatggc caatatcggg cagttcgaac gttcgctcat catcgcggat
     8761 gagggcgctc aggtgcacta cgtggaaggc tgcaccgcgc cgctttacct gaccgagtcg
     8821 attcacagcg gcgtgattga aatcattgtc aaaaaaggcg cgcgggtgcg ctataccacc
     8881 atccaaaact ggtccacgaa cgtctataac ctggtcaccc aacgcgcggt ggtggaagaa
     8941 gacggcacca tggaatgggt ggacgcgaac ctgggatcga aagtgaccat gaagtacccc
     9001 gcgtgtttcc tgaaaggtcc tggcgcgcat ggggaggtgc tttccgtcgc ttttgccggg
     9061 ccggggcagc atcaggacgc gggcgcgaag atgatccatt tcgcgcccaa taccagcagc
     9121 aaaatcacct ccaagtcgat ttcccgcggg aacgggcgct cgtcctatcg cggcttggtg
     9181 cgcgtggctg ccgcagccga aaattcgcgc tccagcgtgg tgtgcgacgc tttgctgttg
     9241 gatcacgagt cgcgttcgga tacatacccg accatggata tcaaggccag ggatgtgcaa
     9301 atcgggcacg aagccaccgt gagcaagatt ggcgaggaac aactcttcta cctgaacagc
     9361 cgcgggctct ccgatgagga ggcgaccacg atggtggttt ccggtttcat cgagccgctg
     9421 gtgaaagagc tgccgatgga atacgcggtg gaaatgaata agctgatcca actgcagatg
     9481 accggctcga ttgggtaggt ggaaagatgc gaaccaacct gcttgagttt ctgaatagca
     9541 acaggcccga actgcctgtg gtggtggcgg gcaggcagcc cctgggcgca gccccaaaac
     9601 tgcttgaatt ccgtcaggca gcctggcggc atgcccaaag tctgccattc ccgggtaaaa
     9661 aggacgaagc ctggcggcgc gtcaacctgg acgatttgct caatacgccg ctgaacatgc
     9721 tgagcgcgaa aaagcaaaag ctgcgtataa acgcgggcat ccccgccaga gaggccgccg
     9781 gctacgcggg cgcgcttttt tccagcccga acggcagcag cctgagcctg gaccctgcgc
     9841 ttgagcaggc aggcgtgctc ttctgcgatt ttactgaggc gcaaaggact cacgcggagt
     9901 tccttgtgga tcatctcggg gaggctgtgc ccccgggcga cggtaaattt gcggcggcgg
     9961 ccagcgcgtt ggcagaattc ggggcggtgc tgaacgttcc caaaggtttt cgagtagaaa
    10021 agccgctgct tgtgcgcctg gaagccagcc aggcaggcag cgccgctttt tcgcacaatc
    10081 tcattcgcct ggaatccggc gcttcggcag tgcttgtttt ggatttcaac tcacccgcag
    10141 ccgcggaggg cagcctgcaa gcgctgcacc tcggcatcct ggagctccaa ctggaagcca
    10201 acgctgagct aaccctggtc gagctgcagc agttctcccg gcagacctgg aacatcaccc
    10261 acgaacgcgc ggttctgggg gaaggagcgc gtttgcactg gatttacggc gcgcttggtg
    10321 ggagcctgag caagaacttc ctagaaacct ccttacaagg agccggagcg gaagcaaaaa
    10381 tgcacggttt ctatttcgcc agcggccgtc aggtctttga cctggacacg cagcaagacc
    10441 acctggcgcc gcataccacc agcgacctgc tctacaaagg cgcggcggct ggtaacgcgc
    10501 ggacagtctg ggaagggatg atttttgtgg cgcctcaggc gatgcagacc gacggttttc
    10561 agactaaccg caatctggtc ttgagcgaag gcgcggaagt gaacgcgctg ccaggcctgg
    10621 aaatcctggc ggatgacgtg cgctgttcgc acggcgcgag cattggaagg ttggatgagg
    10681 acgagctttt ttacctgcag acgcgcggca tcccccgcag agaagcggag cagctggtca
    10741 tggaaggctt ttttggtgag attgtggagc gggtgccagt cgccagcctg cagtcggaat
    10801 tgaagaaatt attgtctgcc agatttaatc aggcggtctg aaagtcagaa gatgagaatt
    10861 ggaggaacaa tgacgaccgg taagtttatg cgttctccag aaccggaaat tacctacgaa
    10921 gtgggtgata aggtcgaagt caattgcgat cacgaaaaga acggccagcg cgtacggggt
    10981 tggcttaggg ggactgtagt gcaggtggat gacaagaacc tggctgtgca gttccgcacg
    11041 aatatttacc tgactgacgg ctggatggtg ccggatcaga ttctgtggtt cccgtttgac
    11101 tccgctaacg tccgtcggat tcagccaaaa gcaaaatcct ccgggaagcc cgattttaac
    11161 ttactttagg tttcaggcag cgggtccgaa agaacccact atccgaataa tatcgaagcc
    11221 ttccgcgaat ggacgctcgg ccagcgcgcc gtgtctgacg gcggtggaac gcagcactgc
    11281 ttccgaaaag taataacgct cggcgtaggt ggtgtacctg gagagggctt cgattttttt
    11341 gttaacgccg gcttccgtca cctccactaa aaaatgcggg aagaaaccgt agctgctgcg
    11401 cagaacgtcg tatcccaaca ctgtcgtacc gcgaaaagcg cgcagagctt cctgggtcag
    11461 ggttacgtgg tcctgatgga tatcctgtgc ggtgtgcaca aagacgattt gcggttggag
    11521 ctggcgcctg agcttcagca ttttttccaa aatttcctgc cgaaaatccg ggaagcggcg
    11581 agtctcaaag gagcctacct caatctggtc gtcgcgcaag cccagggtgc gcatgctgac
    11641 ataatgctcg tcaaggagat gctgtaagtc cgggttcttt ttgttgtcag agaaggtcat
    11701 gcagtacagc tcggtctgac cgacgatatc cgccagaagc gccccgcagc caagttcgat
    11761 gtcgtccgga tgggcgccca ggaagagcac cgactttccg aaaaaggtca tattgttcat
    11821 caggctgctc actttgtggc gatgaaaccg ccgacgacct tggtcatcac caggctgccg
    11881 tccttcgcga tgcgcgccct ggcgaccttt tcaatctggg ccatcacgat ttcaaagtca
    11941 tcatgggtga tgaaaagcga attccagagc ttgcggtctt cggacatgga cgcgcgggta
    12001 taaaccatga aaggctcggc gtccgtaaag gtcagcggat tttcaaagat gtgctgttcc
    12061 actttggaaa agagcttttc catcgtgccg tagatatccg aggcataacg cgagctgccc
    12121 ggcatgggcg ggatagcttt accggttgct tccttgataa tatcgtagaa aatagccttg
    12181 ttggtgggca tcgggccggt agtgaagagc cggccgcccg gtttgagcac gcggtgcatt
    12241 tctgccagcg tgaagggaat atcctgtgcg tagtagattg cgaaacaggc cgatatcaag
    12301 tcgaatgaat cgccgggcag gtcgaaagcc tggttgaaat ccagcagttg gaacctcaca
    12361 ttcgcgccgc gtttttcgtt ttcctgccgg gcctgcgcaa gcaaggattc tgagacatcc
    12421 gtgccggtga tttccgcttc gccatccagg tgctgatgga atgagaaaca ctgtttgcca
    12481 gcgccgcagc ctacgtccag aatgcgaagt ccttttttca gctgcagaat atccagcatc
    12541 cagctgtcaa tgtcgcgcgc gccatattgg ctgtggatat cgatgcgctt gagcaaatcg
    12601 ttggtagttt cctgaaaatc aattttcatt tctttcgcct ttcgtgtgag gttagcaagt
    12661 caagtataca aatcgctatg tttttgtcaa gaaagtgaat aacgagggta ttgcaaaaca
    12721 atatttacct ttcaggatga tttctttttg gtagcatcat tctgcataat acgctgcggg
    12781 tactccagcc tcgaaccttc cccgccggca agcggttttc ttggcgcggt caatccattg
    12841 aggaaacgaa aaaagttcaa tagagagcgc ggctttctct caattttcaa cttccgaccc
    12901 caacgagagt tcatcaggaa cccaggataa atagagaccg ctgggaaaga aaggtagagg
    12961 gaaaaggctg ttcgtgggaa aatcaccggg gttgggtaag atctttcttg atcttttatc
    13021 cacactctga gtgaacaagc tggctgcgca tagattattg aggtgtagtt ttctggaggt
    13081 cggattgcaa ttgctgtgac tgataaagct gcctggtgtc ggggctgacg aatcgacagc
    13141 agattaggcg ggcagccctg ctttaacaaa tgcgcggcag catctcgccg gcaagcatat
    13201 ccaatatgcg ggtagtccca aacgcagtgc gcagcagcag ctgaccgctg gagcgggaaa
    13261 cgacgctgcc gatgcggcgg gcgccagagc cgtatttttg ggtgcgcagg taattcagtg
    13321 cggattcagc ctgcgcttcc ggcaggatga cgatgacctt gccttcgttt gccagataga
    13381 gcggatccaa tccgagcaaa tcgcaggcag cccgcacagg ctggtctatt gggatatccg
    13441 gctcgtttag ctggatggtg acgccggatt gcccggcaat ttctaccagg gtggtcgcca
    13501 acccgccgcg ggtggggtcg cgcattacgt gcaggtccgg aaatgcggcg cacaagcctt
    13561 gcgtcagatg gttgagcggc gcggcgtctg accggacgtc ggactgaaag ccgaggttgc
    13621 cgcgcgcctg gagtaccgcg atgccgtgat ttcccaaagc tccggaaatg agtaccgcgt
    13681 cgccaggctg cgcctggtcg ccggtgattc ttcgtccagc aggggcatag ccgaagccgg
    13741 cagtggtgat gaaaagttta tcggctttgc ctttttccgt aaccttggtg tccgcggcga
    13801 tgattcgcac atcggcttcc gtgcaggctt cccgcatgga ggccaggact gcttcgagct
    13861 ccgggatgga aaggccttcc tccaggatga agctggcggt gaggtacttt ggctgcgcgc
    13921 caagcatcgc cacgtcgttt acggtgccgc agaccgacag cctgccgata tcgccgcccg
    13981 ggaaaaaaag cggaaagacc acatgcgcat cggtggaaac caccaattga aatccgggcg
    14041 gaggggtcag ttcgccgc
//
LOCUS       DAOQAD010000044        13832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_4854_length_13832_cov_6.780794, whole genome shotgun sequence.
ACCESSION   DAOQAD010000044 DAOQAD010000000
VERSION     DAOQAD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13832
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4854_length_13832_cov_6.780794"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2037)
                     /locus_tag="PKL60_04990"
     CDS             complement(<1..2037)
                     /locus_tag="PKL60_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNW95527.1"
                     /translation="MKRTLLLFLVFTLLLGACAKLPASGTSTPTPVSGLPTPVLALTQ
                     APDVESEAKAYLEAWKSEDYEGMYGRLSKLTRDALTLEDFTTQHKNAAVAMTMQEMNY
                     NILSAMVRPSTAQVAYDVSYTTTLLGTIERGTIMNLTLEDGAWQVQWDVSMILPELAG
                     GNKLELTYEIPARGNLYDIYGYPLVAQDDAVALGVIPGQIDPEKEASMLSLLANILDV
                     SSDSIYKKYQYAGPDWYVAIGDISAVTAQKYEEKLSSYPGLILSSFRSRYYYDGGIAP
                     HVIGYVLSISAEELEKYQRLGYRGDEKIGASGLEAWGEPYLAGTHGASLYVKDPQGQV
                     VTKLASAEPKPAASIYTTIDSTLQYYLQRSLGDNLGAIVVVERDTGKVLSMVSNPGFN
                     PNLFEPVNYNSMALNDVLTDAKLPLYNRASQGVYPLGSVFKIITMAAGLETGVFTPDY
                     PYYCDDKWTELAGTVLYDWTYERGFGASGLLSLKEGLMRSCNPWFWHIGLTLWNDGYE
                     SAIADVAAGFGLGKKTGIEIPEFEGRLEQPDSVSANVQLAIGQGTLQVSPLQVAAFVA
                     AVGNGGTLYKPTVVEKIVPVDGTDPVYEFAPVVNGQLPVTAENLKAIQEAMVMVVRNT
                     RGTATYQFRGLSVNIAGKTGTAENPAGDSHAWFAGYTFNEDPNRPDIAVAIILEN"
     gene            complement(2045..2710)
                     /locus_tag="PKL60_04995"
     CDS             complement(2045..2710)
                     /locus_tag="PKL60_04995"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNW95528.1"
                     /translation="MKELKLVLASASPRRRELLGLFGLPYTCQAAGIDENIRDGETPG
                     MYVQRLAREKNEVVRAHALVLSADTIVAHNGSIFGKPADAREAERMLTELREKTHNVY
                     TALCLRGDGRALADCCETSVRMRPYTRAEMDAYIASGDYRDKAGGYAIQHKFFHPVES
                     LDGCYANVMGLPLCHLLCLLRRAGLRIEADIPALCQRHLDIQCEVFPGILARCDADNR
                     DAV"
     gene            complement(2800..3720)
                     /locus_tag="PKL60_05000"
     CDS             complement(2800..3720)
                     /locus_tag="PKL60_05000"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNW95529.1"
                     /translation="MFELVFLGTSASAPSIKRGLSSLVVKHGEHRFMIDCGEGTQRQI
                     LKSGIGFKRLNHILLTHGHLDHILGLAGLISTFMRWEAIEEINIYGSRHTMERVDDLI
                     NGVVLRGAQTPMPIRLLDIKPGLIFETEDFTLHAFQVEHRGSSSLGFRFEEFPRRPFL
                     PEKAEALGIAPGPWRKDLVADKSITLPDGRVIEPDQVLGEEKAGTSLVVVGDVGKPEK
                     LREQVRGADALVIEATYLQEEAAMAQEFGHTTAGAVAELAKQGGVGQLILTHVSRRYS
                     DEEILAEASAVFPNTKLAHDFDAFTIRHEE"
     gene            complement(3751..3948)
                     /locus_tag="PKL60_05005"
     CDS             complement(3751..3948)
                     /locus_tag="PKL60_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95530.1"
                     /translation="MKTSTKTITLIVGTALGALLGYGAARILIRQSEAAGKSSPVSAS
                     QALQVGLGALGLLRQISGGGK"
     gene            complement(3945..4400)
                     /locus_tag="PKL60_05010"
     CDS             complement(3945..4400)
                     /locus_tag="PKL60_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95531.1"
                     /translation="MKETAQSALEPRAPERIHKKERFWQILFPLFVVCGLLTAGFALL
                     LISAGELGSSTEGMAAAALVAIALPLMLIFLILLLVLAALISGAAKALAKLPEAGGEV
                     LRVFRSINASVQNGSRAVAAPLLSIGQKSAEIHQIIAGVRSRLTKGKVR"
     gene            4937..5590
                     /locus_tag="PKL60_05015"
     CDS             4937..5590
                     /locus_tag="PKL60_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNW95532.1"
                     /translation="MEKVITAIEAQKRNRQRVNISLDGEFAFSLDRLTAAWLLPGRQL
                     SAEEITRLQEQDEAESAHTRALHFLSFRSRSAEEMRDYLKRKGCDPELAAGVLARLEE
                     EGYLDDARFCREWVENRSAFRPRGVSLIKVELRRKGIPNETIDSALAQADLQESELAL
                     KAARRVVGRYAALPQETFRLRLGAYLRRRGFSYAVASSVLNRLWKESQNSAHGQDLS"
     gene            5612..7177
                     /gene="rny"
                     /locus_tag="PKL60_05020"
     CDS             5612..7177
                     /gene="rny"
                     /locus_tag="PKL60_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNW95533.1"
                     /translation="MEHLNLYAQGPVFRDPQGILFLVLALALGFALGFVLFSALRKQK
                     EEKLHQTAKDILTAAEERAKQTELDAKDSALKIIRDAEVDLQHRRSELSKEDDRLVKR
                     REELDHRFDQMQQREAALNKRQSTMDRRANEIEGLAAQRMEELQRVSQMSIDEARALL
                     LEEVTQTSRGDMMRIIRQYETEAREMGQEKARELIADAIQRVASDRVAEVTTSLVTLP
                     NEEMKGRIVGRNGRNIRSFEQAAGVDVIVDDTPEAVTISCFDSVRREIAKRTLSRLVV
                     DGRIHPAYIEKILNEERQKMDEDILKAGNEAAYEAGVHGLHPEIIKKLGRLKYRTSYG
                     QNQLAHAVETSKIASVLASELGANVEICKAGALLHDLGKAMDHNTEGTHAIIGAEFAK
                     RHGVNAAIVNVIASHHHEVDQGSVEAVIVEASDAISGARPGARREDLEQYIKRLKSLE
                     AVANAHKGVESSFAIQAGREVRIIVRPDEIDDLAAHEMAREIATEIEETMQYPGQIQV
                     TVIRETRAVTFAK"
     gene            complement(8598..9245)
                     /locus_tag="PKL60_05025"
     CDS             complement(8598..9245)
                     /locus_tag="PKL60_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNW95534.1"
                     /translation="MSLAIVRMVLGPLQNNVYILADEESKTAVVIDPSFEPERQAEEI
                     RRRGWNLRQIWLTHGHFDHTQGAADLQKLFTPRPLVGMHPEAQASAQANGGKLTFGYQ
                     TAPLPDVDIRFFQGQQLGLDPDEGMSTVEVREVPGHSPGSVIFYCAELGAAFVGDAIF
                     RESIGRTDLAGGDYPALVAAIRAQIFTLPDETALLPGHGPATSVGYEKDNNPYLD"
     gene            complement(9254..10498)
                     /locus_tag="PKL60_05030"
     CDS             complement(9254..10498)
                     /locus_tag="PKL60_05030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW95535.1"
                     /translation="MTWSSARMTLTALPTCRATCCCLITSGIDVKERRDLYLVALSLL
                     IWGMGEGLFYIFQPLYIQKLGADPILIGTVLGINAVGMTLSQIPAGYLADRVGRRPVM
                     WFTWMMGVISALVMASARNLSGFVVGMLMYGLTSAVLAPMNSYISHARGDWPVGRAVT
                     FASAAFSIGAILGPLVGGALGERFGLREVYWLAAVLFLVSTVVILFIREQPVEAHSET
                     ESQPGLLRNPRFLMSLGLILMMMFSMYLPIPLAANFLQNERGLSLASIGQLGSLQNLG
                     NAAFALALGHLPALTAFLAGEAALLAVSLLLWRGSGMGFFGLAFFLMGGYRLSRTLSL
                     AFVQPLVAKNQVGLAFGFVETINNLALIMTPVFAGLLYDRSPAMVFKVSAGVLALCLG
                     LTVWLRGAGRQAARAVIHRLQR"
     gene            complement(10413..11528)
                     /locus_tag="PKL60_05035"
     CDS             complement(10413..11528)
                     /locus_tag="PKL60_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNW95536.1"
                     /translation="MTDRLQRFLDLMRQHNIDAAAINPGPTFTYLTGMQMHLMERPTV
                     LLVARSGAAALVLPQLEQLKLAGARLELKPCLYPDNPATWQEAFSDAFRGLGLRKGRL
                     ALEPRGMRYLELTFLQSAAPECELVDGSLVFENLRLLKNQSELDKMQQAAQIAQKALL
                     ETLKTVRPGQTEKQIAAELVIALYRAGSDIELPFQPIVSSGENSANPHASPGERTLRE
                     GDLLLFDWGASVDGYFSDITRTFMVGKVDAELERIGAVVRDANAAGRAAARPGVPASA
                     VDTAARGVITDAGFGEYFTHRTGHGLGMEAHEAPYIRGDNTILLDPGMVFTVEPGIYL
                     PGLGGVRIEDDMVISQDDAYSLTNLPRDLLLLNYQRD"
     gene            11868..12818
                     /gene="cysK"
                     /locus_tag="PKL60_05040"
     CDS             11868..12818
                     /gene="cysK"
                     /locus_tag="PKL60_05040"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNW95537.1"
                     /translation="MTIYQDLTQLTGRTPLVRLNRLVPAGGAEVLAKLEFFNPTHSVK
                     DRIAVAMVDTAENQGLLSPESIILEATSGNTGIGLACTASARGYKSAIVMPDTVSMER
                     KLLLRALGAELILTPGAEGMAGAMEEALNLRAKDSRYWLADQFNNPANVEAHRRTTAE
                     EIWRDTDGNIDILVAGVGTGGTITGTGSKLREYNPALRIIAVEPAASPVLSGGKKGPH
                     PLQGIGAGFVPSILDLRLLDEIIQAEAEDAFFTARSMATVEGIPVGISSGAAVWAALQ
                     VANRAENAGKRIAVIIPSFAERYLSTNLFASIREELNASR"
     gene            12889..13479
                     /gene="cysE"
                     /locus_tag="PKL60_05045"
                     /pseudo
     CDS             12889..13479
                     /gene="cysE"
                     /locus_tag="PKL60_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638459.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
     gene            complement(13623..>13832)
                     /locus_tag="PKL60_05050"
     CDS             complement(13623..>13832)
                     /locus_tag="PKL60_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95538.1"
                     /translation="PALPTPVPTLPGAVQATPDAAQLTATALVSSPPVVHVESIVITG
                     GVLVMIVLAAILREILQFRDSTDEK"
BASE COUNT         3028 a         4144 c         3873 g         2787 t
ORIGIN      
        1 gttctcgagg ataatcgcca ccgcgatatc cgggcggttg gggtcctcgt tgaaggtata
       61 gcccgcgaac caggcgtgcg agtctccggc tggattctcg gcggtgccgg tctttccggc
      121 gatattcacg ctgagcccgc ggaactgata agtcgccgtc ccgcgcgtat tgcgcaccac
      181 cattaccatg gcttcctgga tggccttcaa gttctccgcg gtcaccggca gttggccgtt
      241 aaccaccggc gcgaattcgt acaccgggtc ggttccatca accggtacaa tcttttccac
      301 cacggtcggc ttgtaaagcg taccgccgtt gccgacggca gccacaaacg cggcgacctg
      361 caaggggctg acctgcagcg tgccctgccc gattgccagc tgtacgttgg cggatacaga
      421 atcgggctgc tccaatctgc cctcaaattc cggtatctcg atgccggttt tcttgccaag
      481 cccaaagccc gcggccacgt ccgcgatagc gctttcgtag ccgtcattcc agagcgtcag
      541 tccgatgtgc cagaaccagg ggttgcagga gcgcatcaag ccctctttca ggctgagtaa
      601 gcccgaagct ccaaagccgc gttcataggt ccagtcgtag agcacggtac ctgccagttc
      661 cgtccacttg tcatcacagt aatacggata gtcaggggta aagacgcctg tctccagccc
      721 cgcggccatc gtgataatct tgaacacaga cccgagtggg tataccccct gggaagcgcg
      781 gttatataaa ggaagcttgg catctgtgag cacatcattg agcgccatgc tgttgtagtt
      841 cactggctcg aacagattag ggttaaagcc ggggttggac accatggaaa gcactttgcc
      901 ggtatcccgc tccaccacca caatagcgcc aaggttgtcg cccaacgaac gctgcaggta
      961 gtactgcagg gtgctgtcga tggtggtgta aatcgacgcg gctggtttgg gctcggctga
     1021 agcgagcttg gttaccactt gcccctgggg gtctttgacg tagagtgacg cgccgtgcgt
     1081 gccagccagg tagggttcgc cccaggcctc cagcccgctg gcgccgattt tttcatcccc
     1141 gcggtatccg agacgttggt acttttccag ctcttccgct gaaatggaaa ggacatagcc
     1201 gatgacgtgt ggagcgatgc cgccgtcgta atagtagcgt gaacggaatg agcttagaat
     1261 caagcccggg taggaagaaa gcttttcttc atacttttgc gctgtaacag cagatatatc
     1321 tccaatagcc acataccaat ccggtcctgc gtattggtat tttttataga tggagtctga
     1381 actgacatcc agaatattgg caagcaagct gagcatgctg gcttcttttt ccgggtcaat
     1441 ctgcccgggg ataaccccca gcgcgaccgc gtcgtcctgg gcgaccagag gatacccgta
     1501 aatatcgtaa aggtttccgc gggccgggat ttcataggtc agttccagtt tgttcccgcc
     1561 ggcaagttca ggcaaaatca tgctgacgtc ccactggacc tgccaggcgc catcctccag
     1621 ggtcaggttc ataatcgtac cacgctcaat cgtccccagc agcgtggtgg tgtagctgac
     1681 gtcgtaggct acctgggccg tggaagggcg caccatggca gaaagaatgt tgtaattcat
     1741 ctcctgcatt gtcatcgcaa cggcagcgtt tttgtgctgc gtcgtaaagt cctccagcgt
     1801 aagcgcgtcg cgggtcagtt tgctcaagcg gccgtacatg ccctcgtaat cctcggattt
     1861 ccaggcttcc agataagcct tggcctcact ttccacgtcc ggggcctggg taagcgccag
     1921 cacaggcgtg ggcagtccgg ataccggcgt gggagttgat gtaccggatg caggcaattt
     1981 cgcgcaggca cccagtaaga gcgtaaatac cagaaacagc agaagtgttc ttttcatttc
     2041 catatcagac cgcatcccga ttatccgcat cgcagcgggc gaggatgccg gggaacacct
     2101 cgcattgaat gtccaggtgc cgctggcaga gagcgggaat atccgcttct atccgcagcc
     2161 ccgcccgcct gagcaggcac agcaagtggc ataagggcaa gcccatcacg ttcgcgtaac
     2221 agccgtccag gctttccacc gggtgaaaaa atttatgctg gatggcgtat ccaccggctt
     2281 tgtcgcgata gtcgccgctg gcgatataag cgtccatctc cgcgcgggtg taggggcgca
     2341 tgcgtacgct tgtttcacag caatccgcca gcgcccggcc gtctccgcgc aagcacagag
     2401 ctgtatatac gttgtgcgtt ttttcgcgca gttccgtcag catgcgttca gcttcgcgcg
     2461 cgtcggccgg ctttccgaat atgctgccgt tgtgcgcgac aatcgtatcc gcggaaagca
     2521 ccagcgcgtg cgcccgcacc acttcgtttt tttcccgcgc cagccgctgc acatacatcc
     2581 ccggggtttc accatcccgg atgttttcgt caatgccagc agcctggcag gtatacggaa
     2641 gcccaaacag acccaggagt tctctgcgcc gcggagatgc tgacgccaga actaatttta
     2701 attctttcat agcgaaaaag attctaacat agatagccgg ggagtattcc gctcattcgg
     2761 gctgcgcgaa ggcatggctt tcgcccgaga gcgcggattt tattcctcgt gccgtatcgt
     2821 aaaggcgtca aaatcgtgcg cgagcttggt gttggggaaa accgcgctgg cttcggccag
     2881 gatttcttca tcgctgtagc gccgggagac gtgcgtgaga atcagctgcc ccaccccgcc
     2941 ctgttttgcc agttccgcca cggcgccagc ggtggtgtgc ccgaattcct gcgccatcgc
     3001 ggcttcttcc tgcaggtagg tcgcttcaat caccagcgca tctgcgcccc gaacctgctc
     3061 ccgaagcttt tccggcttgc ccacatcccc gaccaccaca aggctcgtgc cggctttttc
     3121 ctcgcccagc acctggtcgg gttcgattac cctgccgtcg ggtaaagtga tggatttgtc
     3181 tgccaccaaa tctttgcgcc agggcccggg cgcgatgccg agcgcttcag ccttttccgg
     3241 caggaagggt cggcgcggaa attcctcaaa gcggaagccc agggaggacg aaccgcggtg
     3301 ttccacctgg aaagcgtgca gcgtgaagtc ttcggtctca aaaatcaagc cgggcttgat
     3361 atccaaaagc cggatgggca tgggggtttg cgccccgcgc agcaccacgc cgttgatcag
     3421 gtcgtccacg cgttccatgg tgtgccggct gccatagatg ttgatttcct cgatggcttc
     3481 ccaacgcatg aatgtggaaa tgagcccggc cagccccagg atatggtcca gatgcccgtg
     3541 tgtgagcaga atgtggttca ggcgtttaaa accgatgccg gatttcagga tttggcgttg
     3601 ggtgccttcc ccgcagtcaa tcataaagcg gtgttcgccg tgcttgacga ccaacgaaga
     3661 aagccctctt ttaatagagg gagcggaagc ggaggtgccg agaaagacaa gctcgaacaa
     3721 gggttacttg gtttcctgtc agcttctgac ttacttcccg ccgcccgaaa tctgacgcag
     3781 cagccccaac gcgcccaggc ccacctgcag cgcctgggaa gcgctcaccg gggagctttt
     3841 ccccgcggcc tccgactgcc tgatcagaat tctggctgcc ccatacccca ataaggcgcc
     3901 taaggctgta ccgacgatca aggtaattgt ttttgtcgag gttttcatct tacttttcct
     3961 ttggtaaggc gcgaacgcac tccagcgatg atttggtgaa tttcggctga tttttgcccg
     4021 atgctcagca acggcgcggc caccgcgcgg ctgccgtttt gcaccgacgc gttaatggaa
     4081 cgaaagaccc gcagcacttc tccgcccgcc tccggcagct tcgccagggc tttggcagcg
     4141 ccgctgatca gagctgccag aaccagcagc aaaatcagaa aaatcagcat gagcgggagc
     4201 gcgatagcca ctagcgccgc agcagccatg ccttccgtgc tgctgcccaa ctcacctgca
     4261 gaaatgagga gcagggcaaa gccggcggtg agcaagccgc agactacgaa cagggggaac
     4321 aggatttgcc agaagcgttc ctttttgtga atcctttctg gcgcgcgggg ttcgagggcg
     4381 gactgggcgg tttccttcat ggggatattg tagcatgaga acgtatggaa ttgcgctcgc
     4441 cgtgtacgca tcaggagatt aatatttcgc cttgacacgg gcctggtaag cacccggaag
     4501 tggaaaagcg catttttgaa aaataatgta taatcttctt acaaaacatg aattgcccgt
     4561 gagttctgcg cgagcttgct catggagatc atggctttaa gaccagacgc ctgcgcgccg
     4621 gtcttgcctg gatagtaaac cgccggaaaa tccggcttat aaattggtac aaaatgcgca
     4681 ttgattggct tggaaacaac aaaatgatgc ggggttcgtg tgaacgattt cagcctcccc
     4741 cgggcaggct gcccgcggaa cgaacgcacc tttaacgctg ctaaagccgg acgtgttcat
     4801 acgagaaaac gtaccaaaat ataatcttag cctgaacgac agcgccgcgc ccaaccccgg
     4861 gcagagcgtg tgattccgga taagttgctg tcctcaaagc cgtggcctcc cccagaccat
     4921 ggctttgtgg gattaaatgg aaaaggtaat taccgcaatc gaagcccaaa agcgcaaccg
     4981 ccagcgcgtg aatatcagcc tggacggtga atttgctttc tcgctcgacc gtctgactgc
     5041 cgcctggctg ctgccaggcc gccagctttc ggcagaggaa attacgcggc ttcaggagca
     5101 ggacgaggcg gaaagcgcgc acacccgggc gctgcacttc ctcagcttcc gttcccgttc
     5161 cgcggaagaa atgcgggatt atctgaagcg caaaggctgc gatcccgagc ttgccgccgg
     5221 cgtgctcgcc cgcctggagg aagaaggcta tctggacgat gcccgcttct gccgggaatg
     5281 ggtcgagaac cgcagcgctt tccgtccgcg cggcgtcagc ctgattaagg tggaactgcg
     5341 ccgcaaagga atccccaacg agaccatcga ctctgcgctc gcgcaggctg atttgcagga
     5401 aagcgaactg gcattgaagg ctgcccggcg tgttgtcggg cgctacgccg cgctcccgca
     5461 ggaaaccttt cgcctcaggc tgggcgcgta cttacggcgc cgggggttct cctacgcggt
     5521 cgcatcttcc gtgctcaacc gcttatggaa agaatcgcaa aactccgcgc atgggcagga
     5581 cctaagttaa gaatatggag tattggaaaa aatggaacac ctaaatcttt acgcacaggg
     5641 accggttttt cgggacccgc aaggaattct cttcctggtg cttgctctcg cgctcggatt
     5701 cgcgcttggt ttcgtgctgt tctccgcgct gcgcaagcaa aaagaggaaa aattgcatca
     5761 aactgccaag gacatcctca cggcagccga agagcgcgcc aagcaaaccg aattggatgc
     5821 caaagactca gccctcaaaa tcatccggga tgccgaagtc gatttgcagc accgccgaag
     5881 cgagctaagc aaagaagacg accgcctggt caagcgccgc gaagaactgg accaccgctt
     5941 tgaccagatg cagcagcgcg aagccgccct gaacaaacgc cagagcacca tggaccgccg
     6001 cgcgaatgaa attgaggggt tggccgccca gcgcatggaa gaactgcagc gcgtctcgca
     6061 gatgtccatc gatgaagccc gggctctgct gctcgaggaa gtcacgcaga catcgcgcgg
     6121 ggatatgatg cgcatcatcc gccaatatga aaccgaagcg cgcgaaatgg ggcaggaaaa
     6181 agcccgtgaa ctcatcgccg atgccatcca gcgcgtcgcc tcggaccgcg tcgcggaagt
     6241 gaccacctct ctggtgacgc tccccaatga agagatgaag gggcgcatcg tcggccggaa
     6301 cgggcgcaat atccgctcct ttgaacaagc ggccggcgtg gacgtgattg tagacgacac
     6361 ccccgaagcc gtaaccatct cctgcttcga ttcggtgcgc agggaaattg ccaaacgcac
     6421 gctcagccgc ctggtggtgg acgggcgcat tcatccggct tacatcgaaa aaatcctgaa
     6481 cgaagaacgc cagaagatgg acgaagacat cctgaaagca ggcaacgaag ccgcctacga
     6541 agccggcgtg cacggactgc acccggaaat catcaagaaa ctcggccggc tcaagtaccg
     6601 cacctcttac ggtcagaacc agcttgccca cgcggtggaa acttccaaga ttgccagcgt
     6661 gctcgcctcc gaactcggag ccaacgtgga aatttgtaaa gccggcgcct tactgcacga
     6721 cctgggcaaa gccatggacc acaacaccga aggcacccac gccataattg gcgcggaatt
     6781 cgccaaacgc cacggagtga acgccgcgat tgtgaacgtc atcgcctccc accatcacga
     6841 agtcgaccag ggctcggtgg aagcggtcat cgtggaggct tcggatgcca tttccggagc
     6901 gcgccccggc gcccggcgcg aagacctgga acagtacatc aaacgcctca agtcgctgga
     6961 agcggttgcc aacgcccaca agggtgtcga atccagtttc gcaatccagg caggccggga
     7021 agtgcgcatc atcgtccgcc cggatgagat tgacgacctc gccgcgcacg agatggccag
     7081 ggaaatcgcg acggagatcg aagaaacgat gcagtatccg ggacagattc aagtgacggt
     7141 catccgcgag acccgcgcgg taaccttcgc caagtagttg gatctaaaca tcaaaagccc
     7201 ccgcaacccc gggggctttt ttgtttaacg accgggcggc acatgccaga gtcaagattt
     7261 gtcatcgcga ctgcttgcgc gccaatagtc gtaggttgcg tgcagcggcg cagccgcagg
     7321 ttaggtgcag cagcgcagga gcgaaaccta ttagcgctgc aattcccatc aggtctggat
     7381 tatgaccaag acttggttgg ggcatctctc tacgcagcaa attctgcagg cctcaagact
     7441 aaatcgtaat tttgaccaga agcctttata tctgatttca ctgttttgag cgctagctgc
     7501 tcaccatacg cgtcaattct tttcttttgt ttcagcagat tcgcgctttg agaatccgga
     7561 tcctctccag tatttatctt cttttgtttt tttagcatta ttgcagacaa taacaaaata
     7621 atatcgggaa tcgtgaatag aaccgtggga atcaatagaa aactacaaac catcaaggtt
     7681 ttttgctgat ttcaacgctg gctttaacag gtatgggtag aagataaaca catcccaagg
     7741 aaatggattg aaaatagcct tagactccac ttggaaagtg tctccataca tgtgtgttgt
     7801 actctgatca actattctgc caagataaat cggaaatgat aaaccatcag tcgtttcatt
     7861 tttacacaca gcgcattgcg ggctcatttc attacctcta agtaaatctc atttatattg
     7921 gcgtgcggac aagcaggaag atggttattt gtataattta ttatacgcat tctcactacc
     7981 tatgttccgc aggaaaagca tcgactcagc gaagtaaact cttccgcagg cgctcagttt
     8041 gccaagcttc cagttcgcgg tggcaataag cagtctggac tttgcttatg gagaatttgt
     8101 cattgcgaac cgttttgcca ttcattcttg agcctgtgat ttcctgtgct caaggcaaaa
     8161 cggtgcggca atctgccttt tacttttttt tgttggttct gcgtttgatt gttacaaaat
     8221 tctctctgaa acgttacctt cttaatcgta tgccaaagaa tggacctcaa aaaatacaag
     8281 gcggactccg caagggatgc ttcgcagtgc cacgacactt tgccatcaaa cctcaaaacg
     8341 ataaatcccc tccgtctggc aaagggtctc gcagcaactg agtggggacc gtcactgcgt
     8401 accgttttgt ctccatccat ttcttaagcc aagccaggta atcaggcaaa acgatgcgcc
     8461 ataacgcgga actgcttgcg taaactactt ggtctgtctg ttaacctgct tgccgattgg
     8521 gaaacatcat ccctctgcgt atcccctcgc tgaagcctgt accttttaag gcatatgagt
     8581 ggcgaaagaa gacaaaatta atccaaatac gggttattgt ccttctcgta ccccacgctg
     8641 gtggccggcc cgtgacccgg cagcagcgcg gtctcatccg gcagcgtgaa aatctgcgcg
     8701 cggatcgccg cgaccagtgc ggggtaatcg ccgcccgcca ggtccgtcct gccgatactc
     8761 tcacgaaaaa tcgcgtcccc cacaaacgct gcgccgagtt ccgcgcaata aaaaataacc
     8821 gaacccgggc tgtgacccgg cacctcccgc acctcgactg tcgacatccc ttcatccggg
     8881 tcaagcccaa gctgctgccc ctggaaaaac cggatatcaa catccggcaa gggagccgtt
     8941 tggtaaccaa aagttaactt tccgccgttg gcctgtgccg aggcttgcgc ctcagggtgc
     9001 atccccacca gcggcctcgg cgtaaacagc ttctgtaaat ccgccgcgcc ctgggtatgg
     9061 tcaaagtgcc catgcgtcag ccagatttga cgcagattcc acccccgccg cctgatttcc
     9121 tccgcctgcc gttccggctc aaaagacggg tcgatcacca ctgcggtctt gctttcctca
     9181 tccgccagga tgtacacatt attttgcagc ggccccagca ccatgcgcac aatcgcgaga
     9241 gacatcagcg cacctacctt tgcaaccggt ggataactgc ccgggcagcc tgcctgcccg
     9301 cgccgcgcaa ccacactgtc agccccaggc acagcgcaag cacccccgcg ctcaccttga
     9361 agaccatggc ggggctccgg tcgtacagaa gtcccgcaaa aactggcgtc atgattagcg
     9421 ccaggttgtt aattgtttcc acaaagccaa acgccagccc cacctggttc ttagcgacca
     9481 aaggctgcac aaacgcgagg ctaagcgttc gcgagagacg gtaaccgccc atcaggaaga
     9541 aggccagccc aaagaacccc atcccgctgc ctctccacag cagcaggctg actgccagca
     9601 aggctgcttc gcctgccaga aatgccgtca gggcgggcaa gtgccccaac gcgagcgcga
     9661 aagcagcgtt gcccaggttc tgcagcgagc cgagctgccc gatggacgcc aggctcagtc
     9721 cgcgttcgtt ctgcagaaag ttggccgcca gcggtatcgg caggtacatg gaaaacatca
     9781 tcatcaaaat caaaccgagg gacatcagga agcgcgggtt gcgcagcaag cccggttggg
     9841 attctgtctc gctgtgcgct tccaccggct gctcccgaat gaacaggatg acaaccgtgg
     9901 agaccaggaa cagaactgcc gccagccaat acacctcgcg cagcccaaag cgttcgccaa
     9961 gcgcgccgcc aaccagcggg ccgagaatcg cgccgatgct gaacgccgcc gacgcaaagg
    10021 tcaccgcccg ccctaccggc caatccccgc gcgcgtggga aatgtagctg ttcatcggcg
    10081 cgagcacggc cgaagtcagc ccgtacatca gcattcccac cacaaagccg ctcaggtttc
    10141 tggcagaggc catcaccagc gcggaaatca cgcccatcat ccaggtgaac cacatcaccg
    10201 gccgtctgcc cacgcggtct gccaggtaac cggctgggat ttgggagagg gtcatcccca
    10261 ccgcgttaat cccgagcacg gtgccaatca aaatcgggtc cgcgcccagc ttctggatat
    10321 agagcggctg gaagatataa aacagcccct cgcccatccc ccagatgagc agggaaagcg
    10381 caaccaggta aaggtcgcgg cgctccttca cgtcaatccc gctggtaatt aagcagcagc
    10441 aggtcgcgcg gcaggttggt aaggctgtaa gcgtcatcct ggctgatgac catgtcatct
    10501 tcaatccgca cgccgcccaa ccctggcaaa taaatccccg gttccaccgt gaagaccatc
    10561 ccggggtcta gcaagatagt gttatccccg cggatatatg gcgcctcatg cgcttccatg
    10621 cccagcccgt gcccggtgcg gtgggtgaaa tactctccga acccggcatc agtgatgacg
    10681 ccgcgggcgg ccgtatccac cgcgctggct ggcacgcccg gacgggctgc cgcgcgtccg
    10741 gcggcgttgg cgtcccgtac gacggcgccg atgcgttcca actccgcgtc cactttgcct
    10801 accatgaagg tgcgggtgat atccgagaag tagccgtcca cgctggcgcc ccagtcaaac
    10861 aggagcaggt cgccttcccg cagcgttcgc tccccgggag aggcgtgtgg attagcgctg
    10921 ttctccccgg agctgacaat cggctggaag ggcagttcaa tatccgagcc ggcgcggtac
    10981 agcgcgataa ccagttccgc ggcgatttgc ttttcggtct gcccgggccg caccgttttc
    11041 agcgtctcca gaagcgcctt ctgggctatt tgggcagctt gctgcatttt gtccagctcg
    11101 ctttgattct taagcaggcg caggttttcg aacaccaggc tgccgtccac aagctcgcac
    11161 tccggcgcgg cgctctgcag gaaggtcagt tccaggtagc gcatgcctct tggttccagc
    11221 gcgaggcgtc ccttccgcag accaagcccc cgaaaggcat ccgagaaagc ttcctgccag
    11281 gtggcggggt tatccggata caggcagggc ttcagctcca ggcgcgcgcc cgctaatttt
    11341 aattgttcca actgcgggag aaccagcgcg gctgcgccgg accgcgcaac cagcagcaca
    11401 gtcgggcgct ccatcaggtg catctgcatg ccggtcaagt acgtaaaggt cgggccaggg
    11461 ttaatcgcgg ccgcgtctat gttgtgctgc cgcatcaggt caaggaatcg ctgtagtctg
    11521 tctgtcattg gctgctccgg aagattgttg gagatgatta taatgcagcg ctgatacccc
    11581 aggcggccgc cgcgcttcgg gcgggtgctt gcacaaagtt cggtttgacg cgcgttttgc
    11641 ggcagttata atcatcgtat gcaaccatct gaaagcaaag atttcgtatt tttcacgcgg
    11701 tttgcgtgca tgtgtccggg gcggggaagc taaaccgcct cgtctctcgc gttttttcca
    11761 gccagttgag agcagggcgg ccctcccccg caattctctg ctcatggtga tgcccggcat
    11821 cactccaagc tggctgtttt tatttaagca ttctctggag acttacaatg acaatctatc
    11881 aagaccttac ccaacttacc ggccgcaccc cgctcgtgcg cctgaaccgg ctcgttccag
    11941 ctggcggcgc ggaagtgctc gccaagctgg aattcttcaa ccccacccac agcgtcaaag
    12001 accgcatcgc ggtggcaatg gtggacaccg ccgagaacca ggggctgctc tccccggaat
    12061 ccatcatcct ggaagccaca tccggcaaca ccggcatcgg gctggcttgc accgcctccg
    12121 cgcgcggcta caaaagcgcc atcgtgatgc cggataccgt cagcatggag cgcaaactgc
    12181 tcctgcgcgc gcttggagct gaactaattc tgacccccgg cgcggaaggc atggccggcg
    12241 ccatggaaga ggctctgaac ttgcgcgcga aggacagccg ctattggctg gcagaccaat
    12301 tcaacaatcc cgccaacgtg gaggcgcacc gccgcaccac cgccgaggaa atctggcgcg
    12361 ataccgacgg caatatcgat atcctggtgg ccggtgtcgg cacaggcggc accatcaccg
    12421 gcacgggcag caaactgcgc gaatacaacc cggcgctgcg cattatcgcg gtggaaccag
    12481 ccgcctcgcc cgtgctttcc ggaggcaaaa aggggccgca tcccttgcag ggtatcggcg
    12541 cgggcttcgt cccttccatc ctggacctgc gtctgctgga tgaaattatc caggcggaag
    12601 cggaagacgc ttttttcacc gccaggagca tggcaacggt cgaaggtatc ccggtcggca
    12661 tctcctccgg ggcagcagtc tgggcagcgc tgcaagttgc caacagagcg gaaaacgcgg
    12721 gcaaacgcat cgcggtcatc attcccagct ttgccgagcg ctacctgagc accaacctgt
    12781 tcgcgtccat ccgggaggaa ctgaatgcaa gccgctgaac ctatccccta cccccctgct
    12841 gatgtgccgc gcgcgagaag cgaggaaaca ctgggacttt tcaaacggat ggccgaagac
    12901 atcaataccg cgctggaacg cgacccggct gcgcgctcag gctgggttgt gctcagttcc
    12961 tatgctggcg tgcatgccgt ctgggcgcac cgggtttcgc attggctgtg ggaacacagg
    13021 ctgtattggc tggcaaggtc aaacgcgcag atatcgcgct tcttcaccgg cgtcgagatt
    13081 catccggcag ccaaaatcgg ccggcgcttc tttatcgacc acggcatggg cgtggtcatc
    13141 ggcgaaactg ctgaaatcgg cgcggacgta acgatttacc acggagtcac cctgggcggc
    13201 gtgagcctgg agaaggtcaa gcgccacccg acgattgaag accatgtggt catcggcgcg
    13261 ggcgcgaaag tgttggggaa tatcacggtt ggagcacaca gccgcattgg cgcgaacgct
    13321 gtcgtagtca agccggtgcc gccgcactct gtggtggtgg gcgtgcccgg tcagattatc
    13381 cgccgcgcgc acgaagccgc tgagcctccg attgacctga aacacaccga cctacccgac
    13441 gcaatcgcgg actgcatcga ggacgtgaac gcgcgcctgg ccgccctgga agaaaagctg
    13501 gatttacaca gccgccccaa ggctccgtac taccagaaaa aagaaggcgt ctgggaaatt
    13561 agcgaaacag acgatttcac aatctgagcc gaacaaacaa gaccgacgga aagccggtca
    13621 tgttattttt catcggtact gtcgcgaaac tgaaggattt cccgcaggat tgccgcaagc
    13681 acgatcatta ccagaacccc tccggtaatc acgatgctct caacgtgcac caccggcggg
    13741 gaacttacga gcgctgtggc agtcagctgc gccgcgtctg gcgttgcctg aacagcgccg
    13801 ggtaaggtcg ggacgggcgt agggagggcg gg
//
LOCUS       DAOQAD010000045        13680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_4926_length_13680_cov_5.089174, whole genome shotgun sequence.
ACCESSION   DAOQAD010000045 DAOQAD010000000
VERSION     DAOQAD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13680
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4926_length_13680_cov_5.089174"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1154)
                     /gene="mltG"
                     /locus_tag="PKL60_05055"
     CDS             complement(15..1154)
                     /gene="mltG"
                     /locus_tag="PKL60_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNW95539.1"
                     /translation="MSVTKRRSGLPIFLLVLILILGAVLAYGVVLAPARAKTLFGPPD
                     ARLNLVQRSRSALALLDAREELLGSRPGAGKSGKFSISADESVRGICARLETEGYVPS
                     GDAICTYLTYSGFDRMVQAGTFTISENLSPLEIARKIADPAARDIAFTVFAGWRMEEI
                     AAAVDLSGFSFSGQEYLNSAMNPTSEARALLNLPDSATLEGYFLPDSYSFEPDIPLES
                     MISVMYTRFAAAVQDARFQEGLARQNLSLHQAVTLASVIERETRADEEKARIASVFYN
                     RLAADMRLETDPTVQYALGFDSASGTWWKSPLSLEDLAVDSPYNTYLYKGLPPGPISN
                     PTLITLIAAVAPEQTPFYYFRASCDGSGTHVFSITFEEHLNNACP"
     gene            complement(1151..1582)
                     /gene="ruvX"
                     /locus_tag="PKL60_05060"
     CDS             complement(1151..1582)
                     /gene="ruvX"
                     /locus_tag="PKL60_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNW95540.1"
                     /translation="MTKVLGVDPGDVRIGIAISDESGTLARPLSILRHVSRPEDAHRI
                     AEIAQEAEAGAIVVGMPLDAQGEVGPKARSSLRLIEALRAICQLPVIPWDESYSSQKA
                     AKIRIQAGVKRKTRAKPIDDLAAAVILQSYLDRQENVGELA"
     gene            1663..3243
                     /locus_tag="PKL60_05065"
     CDS             1663..3243
                     /locus_tag="PKL60_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNW95541.1"
                     /translation="MLIASHVCKAAIYPDAARVTRTASIPLEKGRTLLEFSDLPADLS
                     LESIRVRAAGVPGTKLEGFSSKLVYLEKIAKSEIQNLIEEVERLNQGIQRQQSRIAAV
                     NARIEHLDNLLKETRSFAFGLTNQKLTISQHFETIAAVSVEREKALTERSDFQLGLIE
                     LQKQFAAKKMELEQLNSQQKTASYQIDLPVVLEASGTLNLELSYLQPGCSWEPNYDFS
                     LRNGKLEILYTASVSQESGENWQDINVELSTSVPRAYAKLPEMKPWYLQKEFIMSLPQ
                     AAPNARMGRARKDVDEGETTMLDLSTTALSSAEATYEEANVDTLGAAARYTLTQKVSL
                     PSQPSPSRFVISNLTLDVEEDRLAVPRLSEEVFRRVKLVNTSKLVFLEGKAALYADDA
                     FIGFVAIPLIPQGGEHRLNFGVDERLEIKREIIKQSAAGKFLQEKRLRNYGYQITITN
                     PTEETISLVLQDQLPVSLHEDIKVRLDRAEPNPDSKDELNRMTWNLTLAPKSKKLVQF
                     QFVVEAPSETVIHGLPKN"
     gene            3296..4027
                     /locus_tag="PKL60_05070"
     CDS             3296..4027
                     /locus_tag="PKL60_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW95542.1"
                     /translation="MELAGKTALITGSGVRLGKAISRTLAENGCQLVLHYNSSAEGSE
                     EVRDYAASLGVKALTYQADLSEPEELRALMRFGTAEFGHLDILINNSAIYLPGKGIDT
                     TPELLEKQFRLNLFAPVLLIREFAAQLPEGLPGKIVNISDAKVFRHQPDHFAYRLTKA
                     AVNEMTLMFALELAPRITVNAIAPGVMLPLAGREGTDMQALAERRIPLKRVGSPEIIA
                     ENVLHILSQDFMTGTIVRVDGGEGI"
     gene            complement(4024..5427)
                     /locus_tag="PKL60_05075"
     CDS             complement(4024..5427)
                     /locus_tag="PKL60_05075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNW95543.1"
                     /translation="MKIVCFSINPIFPDRITGGASKHLMRLVSYLADQGHQVVLLAAE
                     ALGGQEVFSLGERILVKPVLPFHLPFPQPYLVSPADLALICELTADELRGADRFYIHD
                     GELLLPFLYSAVPTISSFRDNFYPESILGSFITQADAIIAVSRFSAETLQASAGRVLP
                     GIASRIHTVLNGMDPEIFHPADPSPILERFGLHPSNQRVLLHPHRPEPGKGLLETIQV
                     VDLLRNQYGYDTLRVLVPQWLEQMNGQAESNFHAQITAELTRRGLTENFIFHPWISQA
                     EMAEYYSAGELTFCLGNMAEAFGNVAYESLVCGTPSLAARVGVHRSQLPDDLLSKVDY
                     GDAQAAAAEADKLLQARTRVSGERLSRIRETFSLDRQLEAYAKIICETEKLPLPESKP
                     FHADDNSAYTLAPWCYASPKGIYHDYHSRYYELPGLQQLVESAEIITPQLRQRFEVPA
                     QLVLEWYKLGLLVPVRD"
     gene            5467..5826
                     /locus_tag="PKL60_05080"
     CDS             5467..5826
                     /locus_tag="PKL60_05080"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNW95544.1"
                     /translation="MDKIIIADLQTTGIIGVKHPERDKPQILFINLVLYRDLHLAGVT
                     DRIADTVSYSTIARAVSEQVSDTQFHTVEALAEHLSQYLLQTFPLEAVRVRVEKPKKV
                     SNTARVGVEIFRRKRAG"
     gene            complement(5823..6392)
                     /gene="folE"
                     /locus_tag="PKL60_05085"
     CDS             complement(5823..6392)
                     /gene="folE"
                     /locus_tag="PKL60_05085"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNW95545.1"
                     /translation="MDFDKIQRATTKILEGIGENPEREGLLKTPSRVAKSYGEILAGY
                     RMNPEELVNNALFTVDYDEMVIVRGIEFYSMCEHHMLPFYGIVHVAYIPSGKVIGLSK
                     IPRIVEMFSRRLQVQERMTIQIADFIQETLQPKGVAVVTEGKHLCMMMRGVKKQDASM
                     TTSHMIGAFREQLSTRMEFMNRISAELKL"
     gene            complement(6516..7073)
                     /gene="raiA"
                     /locus_tag="PKL60_05090"
     CDS             complement(6516..7073)
                     /gene="raiA"
                     /locus_tag="PKL60_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNW95546.1"
                     /translation="MTLKIDVFTKDLDLTDKIHDYATKKVSRLQRFLNEIGDCRVDLA
                     YAKNARNASDRFIAQITIRGKGFILRSEERADGIFAAIDATVDKMTRQIERFKGKRWD
                     HRPEGAVVETPEPVEEAAEETAPVIVRRKSFTLTPMDEAEALEQLQLLGHEDFFVFYN
                     ANTNKINVLYKRRDGTYGLIDPIVG"
     gene            complement(7101..7526)
                     /locus_tag="PKL60_05095"
     CDS             complement(7101..7526)
                     /locus_tag="PKL60_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNW95547.1"
                     /translation="MSRLVQAEDWELDMVVPVPLNEKRREERGYNQAAVLAFPLSLNL
                     KLPMRVRALARIRDTESQVHLNAAERKANVQGAFLADPLLVRGKRVLLVDDVCTTGAT
                     LNAAAEALCSAGCSDVRVLTLAKAIDHRQPVEITTPNKV"
     gene            complement(7864..8001)
                     /locus_tag="PKL60_05100"
     CDS             complement(7864..8001)
                     /locus_tag="PKL60_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95548.1"
                     /translation="MRSQSPKGWGPVEYVLLVILIFLVIFTILSLFWPAIQLFYNANL
                     K"
     gene            8149..8673
                     /locus_tag="PKL60_05105"
     CDS             8149..8673
                     /locus_tag="PKL60_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95549.1"
                     /translation="MSDIYDKVVGEQDFFKKLIAKIPGFSGYVERGDRRMSDKLLREQ
                     VALEFETLYQRVSGLQRDLISQGGLAYIDDLEAAAIKLRQFIDRVRTASYGYAGIFDA
                     VKIKEDDLVQVYQFDNQLLSSGETVSHAIDNVEASIGTDGLPAAIKNLTTAAQTCLET
                     FNQRSDLLKGIAAA"
     gene            8708..9763
                     /locus_tag="PKL60_05110"
     CDS             8708..9763
                     /locus_tag="PKL60_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNW95550.1"
                     /translation="MTRIFDVVEYPSEMADEIVHRFPETGIADLRFGSQVIVRESQNV
                     VFFRDGRALDVLGPGRHTITTANVPLLTNLLGQAFGGRTPFTAEVYYVSMREFADRKW
                     GTPQPIIVRNPGVGLGIALLQGFGTYSFQVKDPQQFVTQLVGAQGAYRTSDIETRLRT
                     MLLSKLQDVLGETTSKSSVLELIGLTEELGAAVRAKAQDDFLAIGLLLKSFYIGNLKP
                     SDKSAEELRQMGMLDMQTYTQLQAADAMRDAAQNPAGGAGLTAGIGAGMGIGSVLSNS
                     LAGMSQAGQTPAAAGSGAAAVPAMPDVMTPSEAAAFLKVSEEDVLAAITAGDLKARKI
                     GSAFRISKDALQEYLKG"
     gene            9855..11000
                     /gene="sucC"
                     /locus_tag="PKL60_05115"
     CDS             9855..11000
                     /gene="sucC"
                     /locus_tag="PKL60_05115"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNW95551.1"
                     /translation="MKLHEYQAKLFFAREGIPVPKSGLANTPIEARQVAAEIGYPVVL
                     KAQVLSGGRGKAGGVRLVRNDSEIESNANLILNSQIRGIPVQRLLVEKAVDYSGEFYL
                     GIVNDRSLAKPILILSSSGGIEIEEVALTAPERIEKAAINPLLGLQNYTVRALASGLG
                     IDYSLWKPLQHIAAALWKLFDRLDATLVEINPLAVTPEGSLMALDGKMTIDDNALYRQ
                     KDFLELSDQIVETPTEQEARKFCLNYIQLNGNIGCMVNGAGLAMATMDCIQQAGSEPA
                     NFLDIGGGATADKVAAALTIIMKDPKVKAVLINIFGGITRCDEVAKGLAAVIPEMPVG
                     LPIVVRLAGTNAEEGRKILENYPQLLQAETLMSAAKIAVRESMQVPA"
     gene            10997..11863
                     /gene="sucD"
                     /locus_tag="PKL60_05120"
     CDS             10997..11863
                     /gene="sucD"
                     /locus_tag="PKL60_05120"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNW95552.1"
                     /translation="MTILLNENAHILVQGITGREGLYHSERMQAYAKNVVAGTSPGHG
                     GEWVLGDKVPVFDTVRDAKESTQADTSVIFVPAYAAADAIMESIEAEIRLIVCISEGI
                     PIKDMLKVRALLEHSESRLIGPNSPGILSPGKVKAGIIPGSIAIPGRVGVVSRSGTLT
                     YEVVQVLKDANIGVSTCVGIGGDPIVGTNFVECLDLFESDPHTDQIILIGEIGGNEEE
                     LAAEFILNQVTKPVYAYIAGRTAPENRRMGHAGAIIEGKTGSARSKIEALLRAGVQLF
                     SYPEEIAEIIRI"
     gene            complement(11911..12147)
                     /locus_tag="PKL60_05125"
     CDS             complement(11911..12147)
                     /locus_tag="PKL60_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNW95553.1"
                     /translation="MEDTFKAVKEVIVEVLKIDGENITPETRFIEDLKADSMDQFFLI
                     DGFCEKFDINISDETARDIRSVKDAVAAIEALKK"
     gene            12226..13254
                     /gene="fni"
                     /locus_tag="PKL60_05130"
     CDS             12226..13254
                     /gene="fni"
                     /locus_tag="PKL60_05130"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNW95554.1"
                     /translation="MDATTSHRKDDHLAINLSKDVSSGASTGLESFSFLHQALPEINL
                     SEVDLSLSLFGKRLRAPILISSMTGGSPKAARYNQLLAAAAQEFGLAMGVGSQRAALE
                     NAELAASYDVRAYAPEALLFANLGAVQLNKGYGFEECKRAVNMIRADALILHLNPLQE
                     ALQPEGDTDFRGLLRRIEAVCEELPVPVIVKEVGWGINASLAKQLLESGVSAIDVAGA
                     GGTSWSQVEMYRQSDPWRVSLAADFRSWGIPTARCLQDIKAELPQALVFASGGLQNGL
                     DIAKTLALGATLGGMAGPVLRAADAGYTRLRELIKTLIAEIRICLFACGKRDLNSFDR
                     SVLLPPVE"
     gene            complement(13314..>13680)
                     /locus_tag="PKL60_05135"
     CDS             complement(13314..>13680)
                     /locus_tag="PKL60_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95555.1"
                     /translation="YGLPEVIEYLFRDNVIPNAKNLWVRLLAETGILGFSLFCAWLSS
                     HFHAAQRLENLRKDPEASAFGLVGKLFLIAVVMEGFSMDSFGLPYLWIALALLISAFR
                     INAAEAAAEKNDQAALPQD"
BASE COUNT         3226 a         3747 c         3693 g         3014 t
ORIGIN      
        1 cttcctccga gaaattaggg acaggcattg ttaagatgtt cttcaaatgt aatcgaaaag
       61 acgtgcgttc cgctgccgtc gcagctcgcc cggaaatagt aaaaaggcgt ctgctccggt
      121 gcaactgctg cgatgagagt gatcagcgtc ggatttgaaa tcggaccggg cggcagtccc
      181 ttatacaggt aggtattgta aggggaatcg accgccaaat cctcaagcga taacgggctt
      241 ttccaccagg tgcccgaggc gctgtcgaaa ccaagggcgt actgcaccgt cgggtcagtt
      301 tccaggcgca tatcagcggc cagacggtta tagaatactg aagcaatgcg ggctttctct
      361 tcgtccgccc gggtctcgcg ctcaataacc gaagccagtg tgaccgcctg atggagactc
      421 agattctgac gagccaaacc ttcctgaaag cgcgcgtctt gcactgcggc tgcaaagcgg
      481 gtgtacatca cggaaatcat gctttcaagc gggatatccg gttcaaaact atacgaatct
      541 ggcaggaaat atccctccag cgtggcagaa tccgggagat tcagcaaagc gcgggcttca
      601 gacgtgggat tcattgcgga gttcaggtat tcctgaccgc tgaagctgaa acccgatagg
      661 tccactgcag cagcgatttc ttccatccgc cagccggcga aaacagtaaa ggcgatatcg
      721 cgcgcggcag ggtcggctat cttgcgagca atctccagcg gactgaggtt ctcagaaatg
      781 gtgaaagttc cggcctggac catgcggtcg aaaccggaat aggtcaaata agtgcagatt
      841 gcatccccgg aagggacata tccttctgtt tccaggcgcg cgcaaattcc ccgtacggac
      901 tcatccgcag aaatagaaaa ctttccggat ttacccgctc ccgggcggct gcccagcagt
      961 tcctcgcgcg catccagcaa agccagcgcc gacctgctgc gctgcaccaa attcaggcgg
     1021 gcgtccggcg ggccaaagag ggtttttgcc ctggcaggcg ccaggaccac cccatacgca
     1081 agcacagcac ccaggatcag gatgagcaca agtaaaaaaa taggaagccc ggaacgccgc
     1141 ttcgttacgc tcatgccaac tctccgacat tctcttgtct atcaaggtaa gattgtaaaa
     1201 tcacagctgc agccaaatcg tcaatcggtt tcgcgcgagt tttgcgcttt acgcctgctt
     1261 gtatgcgtat ttttgctgct ttttgggagg aatagctctc gtcccatggg ataactggca
     1321 attggcaaat tgcccgcagc gcttcgatta acctcaggga tgagcgcgct ttaggtccga
     1381 cctcaccttg cgcgtccaag ggcatgccca ccacaatcgc gccggcttca gcttcttgcg
     1441 ctatttcagc aattcggtgg gcatcttccg gcctggaaac atgacggagg atgctgagcg
     1501 gtcgggcaag cgtaccgctt tcatcactga tcgcaatccc gatgcgtaca tctccgggat
     1561 caacgccaag cactttggtc atgcggcctc caggcgcttc ttgtaaagtt catcggaaaa
     1621 ttatacaata gtttgaaacg agccgaaaaa gggagaagga aaatgttgat tgcttcacat
     1681 gtgtgtaaag ctgccatcta tcctgatgca gccagagtga ccagaaccgc aagcatcccg
     1741 ctcgaaaaag gacgaacact attggaattt tcagatttgc ctgccgacct gagccttgaa
     1801 tcaatccgcg tgcgggctgc cggcgtcccc gggacgaagc tggaaggttt ctcctcgaag
     1861 ctggtctacc tggaaaaaat cgcgaaaagc gaaatccaga acctcatcga agaggttgaa
     1921 cgcctgaacc agggcatcca aaggcagcaa tcccgaatcg cggcagtgaa tgccaggatt
     1981 gaacacctgg ataatctgct aaaggaaacc cgcagctttg ccttcggcct caccaaccag
     2041 aagctgacaa tatcccagca ttttgaaacg attgcggcag tctcagtcga aagggagaaa
     2101 gccctcacgg aaagatctga ctttcagctc ggtttgattg agctgcagaa acagttcgcc
     2161 gcgaaaaaga tggaactgga acagttaaat tcccagcaga aaaccgcctc ctaccagatt
     2221 gacctgccgg tggtgttgga agccagcggt acgctgaacc ttgagctgag ctacctgcag
     2281 cccggctgct cctgggaacc aaactacgac ttttccctgc ggaatggcaa gctggagatt
     2341 ttgtatactg ccagcgtatc ccaggaaagc ggggaaaact ggcaggatat caatgtggaa
     2401 ctgagcacat ccgtcccccg cgcctatgcc aaactgccgg aaatgaagcc gtggtacctt
     2461 caaaaggaat ttatcatgtc gcttcctcaa gccgccccca atgcccgcat gggccgcgcg
     2521 aggaaagacg ttgatgaggg tgagacaaca atgctggatc tcagcacaac tgcattatcc
     2581 tcagccgaag cgacttatga agaagccaat gttgacacac ttggcgcagc agctcgctac
     2641 accctcacgc aaaaagtatc gcttcccagc cagccctccc cgtcccggtt tgtcatctcg
     2701 aacctcacgc tggatgtgga agaagaccgc ctggccgttc cgcgcctttc agaagaagtt
     2761 ttccgccgtg ttaagctggt gaacaccagc aaactggttt tcctggaagg gaaagcggcg
     2821 ctttacgcgg atgatgcttt catcggtttc gtcgcaatcc cgctaatccc tcagggaggc
     2881 gagcaccgcc tgaattttgg cgttgatgag cgtttggaaa tcaaacggga aatcatcaaa
     2941 cagtcggcgg caggcaagtt tctgcaggag aaacgcctga gaaattacgg ctatcaaatc
     3001 acgatcacga atccgacgga ggaaacgatt tctctcgtcc tgcaggatca actgcccgtc
     3061 tctctgcatg aagacattaa ggtccgcctg gatagagcgg agccgaaccc tgacagcaag
     3121 gacgaactga atcggatgac ctggaatctg acgctggcgc caaaatcgaa aaaactcgtc
     3181 caattccaat tcgtggtgga ggcgccttca gaaacggtaa tccatgggct tcccaagaat
     3241 tagtggcttc cattttttat gatcgggatt tccggaatca ggaaaggagc gcacagtgga
     3301 attagcagga aaaaccgcat taataaccgg cagcggcgtg cgcctgggga aggcgatttc
     3361 ccgcacgctg gctgaaaatg gctgccagct tgtgctgcat tacaacagct ccgctgaggg
     3421 ttcagaggaa gtcagagatt acgctgcttc gttgggagtc aaagcgctga cttatcaggc
     3481 ggatctgagc gagccggagg aattgcgcgc gcttatgcgt ttcggcaccg ccgaattcgg
     3541 ccatctggat attctaataa acaattccgc gatttacctg cctggcaaag ggatagacac
     3601 gacgccggag ctgctggaaa agcagttccg gctcaacctc tttgcgccgg tactcctgat
     3661 tcgtgagttc gccgcgcagc tgccggaggg tttacccggg aaaatcgtca acatttccga
     3721 tgccaaggtg ttccggcacc agccggacca tttcgcttat cgccttacca aagcggcggt
     3781 aaacgagatg acccttatgt tcgcgctgga acttgccccc cgaattacgg tcaacgcgat
     3841 tgctccgggc gtgatgctgc cgctggcagg tcgcgaaggt accgacatgc aggctctggc
     3901 tgagcggcgc attccgctca agcgcgtggg cagcccggag attatcgccg aaaacgtcct
     3961 gcacatcctt tcacaggatt ttatgactgg cacgattgtc cgcgtcgacg gcggggaagg
     4021 aatttaatcc cggacgggaa ccagcagccc gagcttgtac cattccagaa ccagctgtgc
     4081 gggtacctca aagcgctggc gcagttgcgg agtgattatt tcagcgctct ccaccaattg
     4141 ctgcagcccg ggcagctcgt agtacctgga atggtagtcg tggtagatgc ctttagggct
     4201 cgcgtaacac cagggcgcca gggtataggc gctattatcg tccgcgtgaa agggcttgct
     4261 ttccggtaaa ggaagctttt cggtttcaca aataattttc gcgtaagctt caagctgcct
     4321 gtctaacgaa aatgtctcgc ggattctgct gagccgctcc cctgaaaccc gcgtccttgc
     4381 ctgcaagagc ttgtccgcct cagccgcggc agcttgcgcg tcgccgtagt ccaccttact
     4441 gagcagatca tccggcagtt ggctgcggtg cacgcccacc ctggcggcaa gagacggcgt
     4501 gccgcacacc aaagattcat acgccacgtt cccaaaggct tccgccatgt tcccaaggca
     4561 aaacgtcagc tccccggcgg aataatactc ggccatctcg gcctggctta tccaggggtg
     4621 gaagatgaag ttttccgtga gtccgcgcct ggtcaattcc gcggtgattt gggcgtgaaa
     4681 attgctttcg gcctggccgt tcatctgctc cagccactgc ggcaccagca ccctgagagt
     4741 atcgtacccg tattggttcc ggagtaagtc aacgacctgg atggtttcga gcagcccctt
     4801 gcccggttcc ggccggtgcg gatgcaggag gacgcgctga ttggatggat gaagcccgaa
     4861 tctctcgagt atcgggctgg ggtcagccgg gtgaaaaatc tccgggtcca taccgttcaa
     4921 gacggtatga attcgcgagg caatccctgg caaaacccga cctgcggaag cttgaagcgt
     4981 ttccgcggag aacctggaaa cggcgatgat tgcatcagcc tgggtgatga aggagcccag
     5041 aatggattcc gggtagaaat tatcccgaaa agaactgatg gtggggaccg cactgtacag
     5101 aaatggaagc aggagctcgc cgtcgtggat gtaaaacctg tccgcgccgc gcagttcatc
     5161 cgcggtcaac tcgcaaatca gggccagatc cgccggcgag acgagataag gctgcggaaa
     5221 cggcagatgg aaaggcagga cgggtttgac caggattctt tcgcccaacg aaaaaacttc
     5281 ctgcccgcca agcgcttccg cggcaagcag gaccacctgg tgcccctggt ccgccaggta
     5341 ggaaaccaga cgcatcagat gcttggacgc gccgccggtg atgcggtcag gaaagattgg
     5401 gttgatagaa aagcagacaa ttttcacgcg attttccatt cgaagtacaa taaagcggag
     5461 gttgacatgg ataagatcat catcgctgac ttgcaaacca ccggtatcat cggggtcaaa
     5521 cacccggaac gcgacaaacc gcagatcctg ttcatcaact tagtcctgta tcgcgacctg
     5581 catctcgcag gcgtcaccga caggattgcc gatacagtca gttattcgac cattgcccgg
     5641 gcggttagcg aacaggtttc cgatacccag tttcacacgg tcgaggcgct tgcagagcat
     5701 ctgagtcaat acctgctcca aacttttccc ctggaagctg ttcgcgttcg cgtcgaaaag
     5761 cccaaaaagg tctcgaacac agcccgtgta ggggtcgaaa tcttccgccg gaaaagagct
     5821 ggctaaagtt tcagttccgc agatattctg ttcataaatt ccatccgcgt cgaaagctgt
     5881 tctctgaacg cgccaatcat atgcgacgtg gtcatgctgg catcctgttt ctttacgccg
     5941 cgcatcatca tgcaaagatg cttaccctcc gtcaccactg ccacgccctt gggctgtaag
     6001 gtctcctgga tgaaatccgc gatttggatg gtcatgcgct cctgcacctg caaacgacga
     6061 gagaacattt ctacaattct ggggattttc gagagcccga ttactttccc ggaagggata
     6121 tacgccacgt ggacaatgcc gtagaaaggc agcatgtgat gttcgcacat actgtaaaac
     6181 tcaattccgc gtacaatgac catctcgtcg taatccacgg tgaacaaggc attgttgaca
     6241 agctcctcag gattcattcg gtagccagcc aggatttccc cataggattt agccacgcgc
     6301 gagggagtct tcagcagacc ctcacgctcc ggattctccc cgatgccttc caaaattttc
     6361 gtggttgccc gctggatctt gtcaaaatcc atttcggttt cgcagaaaag atctgccgct
     6421 ttgaataaag gtttatcgca ttccataagt actcctgcat ctatcttaat caaaaaggca
     6481 gcccttcgaa agggctgcct aacctttgta tggggttaac ctacgattgg atcgattaag
     6541 ccgtaggttc catcgcggcg cttgtagagc acgttgatct tgttggtgtt ggcattgtaa
     6601 aacacaaaga aatcttcgtg cccaagcagc tgcagttgtt ccagcgcttc cgcctcgtcc
     6661 atgggagtaa gcgtaaagct ctttcggcgc acaatcactg gcgcggtttc ttccgcggct
     6721 tcctcaaccg gctcgggagt ttctaccacc gctccttcag ggcggtgatc ccagcgtttg
     6781 cccttaaagc gctcgatctg gcgggtcatt ttatcgaccg tcgcatcgat ggcggcaaaa
     6841 atcccgtcag cgcgttcttc agagcgcagg ataaacccct ttccccggat tgtgatttga
     6901 gcaataaagc ggtccgaagc gttcctggcg ttcttggcgt aagccaggtc cactctgcag
     6961 tccccgattt cattcaggaa cctttgcaat ctggatactt tctttgtcgc gtaatcgtga
     7021 attttatccg tgaggtctaa atctttcgta aaaacatcga ttttcagtgt cataggcacc
     7081 tcctctgggt tataaccaaa ttataccttg tttggagtcg taatttcaac tggttggcga
     7141 tggtctatgg cttttgccag cgtcagcacg cgcacatcgc tgcagcccgc gctgcaaagg
     7201 gcttccgcag cagcgttcag ggttgcccct gtggtgcaga cgtcatccac cagcagcacc
     7261 cttttccccc gcacgagaag agggtcagcc agaaaagcgc cctgaacatt ggctttcctc
     7321 tccgcggcgt tcaggtgaac ctgggattct gtatcccgta tccgcgccag agctctcacg
     7381 cgcatcggga gcttcaggtt gagagaaagt ggaaatgcca gcacggcagc ctggttatag
     7441 ccgcgctcct cacgccgttt ttcgttaagc ggaaccggca caaccatatc aagctcccaa
     7501 tcttcagcct ggaccaatct gctcattaat tcagatacca taagggataa acccaggtca
     7561 gccttatatt tgagatttac gacaatcttc tggatagtcc cctcgtagat agccaggctg
     7621 cgcagctccg taaaagcggg gggctgctga aggcagccct tgcacacccc acgacttcgt
     7681 aagcttctcc cgcatctgac acagctctcc cggctggtaa actggatttt ctgcagacaa
     7741 tcggggcaaa tctgataccc ttcagcgccg cagccaacgc agttcggcgg gaagacccaa
     7801 tcgaggcagt tccaaaatgc cttatagcca ctctgaagca gtgcgtactt atccgccatc
     7861 gttctatttc agattggcgt tgtagaaaag ttggatagct ggccaaaaaa gtgaaagaat
     7921 ggtaaagatg accagaaaaa tgaggatgac cagcagaaca tattccaccg gcccccaacc
     7981 tttaggggat tgtgaacgca tcagactctc cgaggggcat aaggtattga gttcaattat
     8041 aattacattc tcgagcaccc taacaccgct atataatctg ttgttttttc ctatggatta
     8101 ctgtatgata cagaagaatc gtcactattc ggtttaaaag gagcaataat gagtgatatt
     8161 tacgataaag tagtcggtga acaggatttc ttcaaaaagc tcatcgcgaa gattccaggt
     8221 tttagtggct acgtagaacg cggcgaccgc agaatgtccg ataaattact gcgcgagcag
     8281 gtggcgttgg aatttgaaac cctctaccag cgtgtttccg gccttcagcg ggatttgatt
     8341 tcccagggtg gtctggcata tattgacgac cttgaggcgg ctgcaatcaa gctgcgccaa
     8401 ttcattgaca gggtgcgcac cgcatcctat ggatatgccg gcattttcga cgctgtcaaa
     8461 atcaaggaag acgatttggt acaggtctat cagttcgata accagctgct cagctctggt
     8521 gagacagtct cccatgcgat tgacaacgtg gaagcttcca tcggcacaga cggactgcct
     8581 gccgcaatca agaaccttac gactgccgcg cagacttgcc tggagacctt taatcagcgg
     8641 tcggacctgc taaaaggcat cgcggctgcc tgacatttga tgtggcaatg gatttggagg
     8701 aatcaaaatg acaagaattt ttgatgttgt tgaatatcca agcgagatgg cggatgaaat
     8761 cgttcatcgc ttcccggaaa ccggcattgc tgacttgcgc tttggctcgc aggtcatcgt
     8821 ccgcgaatca caaaacgtag tgttcttccg cgacgggcgc gcgctggatg tgttaggccc
     8881 cggccggcac accatcacca ccgctaacgt cccactgctg accaatcttt tgggacaggc
     8941 ttttggcggc cgcacgcctt tcacggctga agtctattat gtttccatgc gggaatttgc
     9001 tgaccggaaa tggggcaccc cccaacctat catcgtgcgc aacccgggcg ttggtctggg
     9061 catcgcgctg ctgcagggct tcggtacgta tagtttccag gtaaaagacc cccagcagtt
     9121 cgtcacgcag ttggttggcg cgcagggcgc ttatcgcact tctgatattg aaacccgctt
     9181 gcgcaccatg ctgctttcca aactgcagga cgtgctcggc gaaaccactt ccaagagcag
     9241 cgtactcgaa ttgatcggcc tgaccgaaga gttgggcgcc gcggtccgcg cgaaagccca
     9301 ggacgatttc ctcgccatcg gcttgctgct caagtctttc tatatcggca atctaaagcc
     9361 ctccgataaa tccgctgagg aactgcgcca gatgggtatg ctcgacatgc agacctacac
     9421 ccagctgcaa gccgcggatg ccatgcgcga tgccgcccag aatcctgcgg gcggtgccgg
     9481 ccttactgct ggcatcggcg cgggcatggg cattggcagc gtgctcagca attcgctggc
     9541 gggcatgtcc caggccgggc agacccctgc cgctgccggc tcaggcgccg ccgcggtgcc
     9601 ggccatgccg gatgtgatga ccccctcaga agccgccgcc ttcctgaaag tctcggagga
     9661 agacgtgctg gctgccatta ccgctggtga tttgaaggcc cgcaagattg gcagcgcatt
     9721 cagaatcagc aaagacgcct tgcaggaata cttgaaaggc taattcaacc agaaaaggag
     9781 cacagcccgg gcatttcccg ggctgttttt atgctagttc gggtaaaata aaagaaaagc
     9841 tctactggag tgcaatgaaa cttcacgaat accaggcaaa attgtttttc gctcgtgaag
     9901 gaattcctgt accaaaatcc ggactggcga acaccccgat tgaagcccgg caggtggcag
     9961 ccgaaattgg ataccctgta gttcttaagg cccaggtgct ttccggtggg cgcggcaaag
    10021 ccggcggcgt cagacttgtg aggaacgaca gcgaaattga atcgaatgcc aacctgatct
    10081 tgaacagcca gattcgcgga ataccggtcc agcgcctttt agtggaaaaa gcggtggatt
    10141 attccgggga attctatctt ggtatcgtta acgaccgttc cctggccaag cccatcctga
    10201 ttctctcatc ctctggcggc atagaaatcg aagaggtcgc gctgacagct cctgaacgaa
    10261 ttgagaaagc ggccatcaat cctctgcttg ggctgcagaa ctacacagtc cgcgccctgg
    10321 catccggatt gggtattgat tactccctct ggaaaccact gcagcatatt gcggccgcac
    10381 tttggaagct ttttgaccgc ctggatgcca cgctggtgga aatcaatccg ctcgcggtca
    10441 cccctgaggg gtcgttgatg gcgttggacg ggaaaatgac tatcgatgac aatgcgctct
    10501 accgacagaa agattttctc gagttatccg atcaaattgt cgagacgcct accgagcagg
    10561 aagcccgcaa gttctgcctg aattacattc agttgaacgg aaacatcggc tgcatggtca
    10621 acggcgccgg gctggcaatg gcgacgatgg actgcatcca gcaggctggc agcgagccgg
    10681 ccaatttcct ggatattggc ggcggcgcta ccgccgacaa ggtggcggcc gcgctcacta
    10741 tcattatgaa ggaccctaaa gtgaaggccg tcctgattaa cattttcggg gggatcaccc
    10801 gctgcgatga agtggcgaaa gggctggcgg ctgtcatacc tgagatgccg gttggtctgc
    10861 ccatcgttgt gcgcctggcg ggcacaaacg ctgaagaggg tagaaaaatc ctggaaaact
    10921 acccccaact gctccaagca gaaacattaa tgtccgccgc gaaaatcgcc gtgcgggagt
    10981 ccatgcaggt gccggcatga caatcctatt gaatgagaac gcacacatcc tcgtccaggg
    11041 gattaccgga cgcgaaggac tgtatcacag cgaacgcatg caagcctacg cgaagaacgt
    11101 cgtcgcgggc acctcacccg ggcacggcgg cgaatgggtt ttgggggata aagtgccggt
    11161 attcgatacc gtccgggatg ccaaagagtc cacccaggcg gataccagcg tgatattcgt
    11221 gcccgcttac gctgccgcgg acgctatcat ggagtccatc gaagcggaaa tccgcctgat
    11281 tgtgtgtata tcggaaggaa tcccgatcaa agatatgctc aaagtccgcg ccctgctgga
    11341 acattcggaa agccggctga tcggaccaaa ctcccccgga attctctctc ccggaaaggt
    11401 caaagctggc attatccctg gttcgattgc gattccaggc cgcgtcggcg tcgtgtcccg
    11461 ctctggaacg ctgacctatg aggtggtaca ggttctcaag gatgccaaca ttggcgtttc
    11521 cacctgcgtg ggcatcggcg gcgacccgat tgtcgggact aattttgtcg agtgtctgga
    11581 tctgtttgaa tccgatccgc atacagacca gatcatcctg attggcgaaa tcggtgggaa
    11641 tgaagaagaa ctggcggctg agtttattct taatcaggtt accaagccgg tatacgcgta
    11701 cattgccggc agaaccgcgc ccgaaaaccg ccgcatgggg catgctggcg caatcatcga
    11761 aggcaaaaca ggctccgcaa ggagcaagat tgaggcgctg ctgcgcgccg gtgtgcaatt
    11821 gttttcttat ccggaagaaa tcgcagaaat tatccgaatt taagaaaaaa aaagaggagc
    11881 cgaaagctcc tctactgtaa tcagaaagaa ttacttcttg agtgcttcaa tcgcggcgac
    11941 cgcgtctttg actgaacgga tatcccgggc ggtttcgtcc gaaatattaa tatcgaactt
    12001 ctcgcagaag ccgtcaatca gaaagaactg atccatcgag tcagctttca aatcttcaat
    12061 gaaacgggtc tccggagtga tgttctcgcc gtcaattttc agtacttcaa cgataacttc
    12121 ttttacagcc ttgaatgtgt cttccatgtg cgcctctacc tcaaaggatt ttgtttattg
    12181 gtctgtcata ttataatgtc aataatataa cacttttatc aaactatgga tgcaactacc
    12241 tcccacagaa aagacgacca tctcgcaata aaccttagca aggacgtgtc ttcgggcgcg
    12301 agcacggggc ttgaatcctt cagtttcctg caccaggcct tgcctgagat taacctctcg
    12361 gaggttgatc tctccctgag cctgtttgga aagcgtctgc gcgcgccaat attgatttcc
    12421 tcgatgactg gcggcagccc gaaagcagcg cgttacaatc aacttttggc tgccgcggcg
    12481 caggaattcg gcctggccat gggggtcggc tcacagcggg ccgcgctgga aaatgctgaa
    12541 ttggccgcca gctacgatgt gcgcgcttac gcgccggagg cactactctt tgcaaatctt
    12601 ggcgctgtgc agttgaacaa aggttatgga ttcgaggaat gcaagcgggc agtcaacatg
    12661 atccgggcgg acgcgctgat cctgcacctg aaccccctgc aggaggctct gcagccggaa
    12721 ggcgacacgg atttccgcgg cttgctgcgg cgtatcgaag ccgtgtgtga ggaactgcct
    12781 gtgccggtta ttgtcaagga agtcggttgg gggattaacg cctcgcttgc caaacagctc
    12841 ctcgagtcag gcgtctctgc tattgatgtg gcaggagccg gcgggacttc ctggtcccag
    12901 gtggaaatgt accgccaaag cgacccgtgg cgcgtcagcc tggccgcgga ctttcgctcg
    12961 tggggcatac ctactgcccg ctgcctgcag gatatcaagg cagaattacc gcaagctctg
    13021 gtctttgctt ccggcggctt gcaaaatgga ttggacattg ccaaaacgct cgcgctcggg
    13081 gcaactttgg gtggtatggc tggtccggtt ctccgcgccg cggacgcggg atacacgcgc
    13141 ttgcgagagc tcatcaagac gctgatcgca gaaatccgga tttgtctgtt tgcctgcggc
    13201 aaacgcgacc tgaacagctt tgaccgctct gtcctactgc cgccggtcga atagttaagc
    13261 ctgaaaccct aaagccccgc gtgttcgact ttattttatc cggcgtgtgt aaactaatcc
    13321 tgaggaagcg ccgcctggtc gttcttttca gcagctgcct ctgcggcgtt gatgcggaac
    13381 gcgctgatga gcagcgccag ggcaatccac aggtacggca aaccaaagct gtccatactg
    13441 aaaccttcca ttaccaccgc tatcagaaac agctttccaa ccaggccaaa agcgctcgct
    13501 tccgggtcct tgcgcagatt ctcaagccgt tgggctgcgt gaaaatgcga gctcaaccag
    13561 gcgcaaaaca gcgaaaagcc aagaatcccc gtctcagcaa gcaggcgcac ccacaggttt
    13621 ttggcattgg ggatgacgtt atcccggaag aggtattcga tgacctcggg aaggccgtag
//
LOCUS       DAOQAD010000046        13281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_5134_length_13281_cov_6.619235, whole genome shotgun sequence.
ACCESSION   DAOQAD010000046 DAOQAD010000000
VERSION     DAOQAD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5134_length_13281_cov_6.619235"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..435)
                     /locus_tag="PKL60_05140"
     CDS             complement(76..435)
                     /locus_tag="PKL60_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95556.1"
                     /translation="MNFISHENTLLQCGSFNETKAYVVEYSLWLELTKEQRQRMVDHQ
                     FPVFIIHKGSRSTASLWRFSKAVLRAFRTQREEQVIPPMGCYPDKRIYKVWKFVERQP
                     QGWTAKLACAAPWEVGT"
     gene            complement(432..893)
                     /locus_tag="PKL60_05145"
     CDS             complement(432..893)
                     /locus_tag="PKL60_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW95557.1"
                     /translation="MEMCVPKISIVPCTYNSEKYLSKALDSVERQSSGNLARIINASY
                     SSDRIPAILRDYTARNAGRYPIRCIQSEAKGAATALNTDSEVASGELIHSLHSEDYSL
                     DEHALARRAALSQQSRIGVVDRQFSGANPRQALENPADPLSQAGCGARFRR"
     gene            1022..1912
                     /locus_tag="PKL60_05150"
     CDS             1022..1912
                     /locus_tag="PKL60_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNW95558.1"
                     /translation="MRASRLLLLPLLPVLVFTGCAPSPGAPTRTTPSETAPTRAALPT
                     TALNTPPAPEGMVFIPAGEFQMGCDPARNSGYSCPADELPIHTLTLKAFFIDRYEVTN
                     AGYNDCVLAGGCRPPSVLSSETREHCYDISVFGDFPVINVSWKDADAYCRWAGKRLPT
                     EAEWEKAARGGQNAAFPWGNDQPSCILANAFDPLSGHLCTGDTTKVGTHPDGASAFGV
                     EDMAGNVWEWVSDWYSETYYAESPAIDPGGPQGDTVKALRGGSWGSRPPSLRASGRSF
                     ELSFYNSSDIGFRCAMDSQP"
     gene            1992..2633
                     /locus_tag="PKL60_05155"
     CDS             1992..2633
                     /locus_tag="PKL60_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNW95559.1"
                     /translation="MPAENTPSQPDPVIRLFKSDFLEFFTHISPVAVLVVFLPLIGFL
                     LWRAASVNSTAWWNVPLAFLIGLAVWPLVEYLLHRFVFHFKPANPSERMQRFLFLMHG
                     VHHAQPREKTRLVMPPVVSLPMAALFYGLFWLVIGKLLGAPGYVDGIAGGFLFGYVLY
                     DMTHYALHHFSLKGAYFRKLRRHHMAHHFKTHDAKYGVSTWFWDDVFKTNPAE"
     gene            complement(2663..4201)
                     /locus_tag="PKL60_05160"
     CDS             complement(2663..4201)
                     /locus_tag="PKL60_05160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNW95560.1"
                     /translation="MSAKPKQTKASPIPAPNRLLELLIPLAGALAVMLPVAPAFAPGV
                     NDNSGVYLYAGWRILQGDLPYLQLWDHKPPLVFYINALGAALSPGSHWGVWALELLAL
                     MLAAYLGYKLIKAHFGSLAAALGTVLWLQALFFIFLGGNQTTDWTLPLQFGALLLFTQ
                     AESRAKPERAYLLIGILGGLAFLIKQTAVGIWLALGILLLAWALRRKPAAWRKLGWMA
                     AGVLAVVLLAGLYFLSKGALAEAWDAAFAYNFHFSASAAQSPADRLRNAVDLRYIAHL
                     PIFPIALLGVLAGAFFPRKKQEPLKRSLFALMLLDGALELGLILMTGDVSKYYFETLL
                     PALALFSAAAFSVLDQWLALSGGRKISRGAVGALAAALLVLGFGLETAERAGEYQQSL
                     TARQAAIRYVRENTSPEDSVLVWRDTRVNYFAQRESPTRYLVMRPLMDADASGQARAL
                     EFLDELLDEKPLLMIDTKLEGWAFLDFPGENAQIKEKVRLLMSFYEPEEMIDNWVVYR
                     LRGE"
     gene            4354..4737
                     /locus_tag="PKL60_05165"
     CDS             4354..4737
                     /locus_tag="PKL60_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNW95561.1"
                     /translation="MQLTHTALYVRDLEAMKDFYIRCFDAQASQKYHNRKTGLQTYFL
                     SFGAGARLELMTRPDLSERDGAAQTYGYAHIAFQVGSREAVDELTCTLAGVGCLVLSR
                     PRITGDGYYEASLQDPEGNLLELVA"
     gene            4753..5622
                     /locus_tag="PKL60_05170"
     CDS             4753..5622
                     /locus_tag="PKL60_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HNW95562.1"
                     /translation="MRSEGTMFSLITDCALKDPRVRLVLLSGSRVNPAAPRDDLQDFD
                     IVYFVADLDSYRASEAWLDTFGSRMMMQKPEEMGANPPEKPDLAYLMLFEDGNRIELS
                     LRPLSRIRETAEGEGFWRVLLDKDQLAASLPKPTAAQFQIQPPSAKTFEDCCNEFWWV
                     SPYVAKGLCRQEQLYAVWHMEAAIRKELLKMLAWLVGLREGWQVQPGKQYKYLQAHLD
                     PSDWKLLTTTCRLDKPANCWQALFAAQRLFRHASRQVGEQFGFAYPPYDGKLSPYIEA
                     LYQRWQDFSSRPR"
     gene            5673..7331
                     /gene="hutU"
                     /locus_tag="PKL60_05175"
     CDS             5673..7331
                     /gene="hutU"
                     /locus_tag="PKL60_05175"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNW95563.1"
                     /translation="MSEPRTVRAPRGTQLSCKNWLIEAAYRMLQNNLDPEVAEKPAEL
                     VVYGGRGQAARSWEAFDAILRSLRALEPDETLLVQSGKPVAVFKSHPDAPRVLIANSN
                     LVPHWATQEHFDALAAKGLIMYGQMTAGSWIYIGTQGILQGTYETLGSLAKQKGWPSL
                     KGKFVLTAGLGGMGGAQPLAVTMNEGVGLIVEVDRERAQRRLEIGYVDALTDDLEDAM
                     TMVEEAVKLGIPKSIGLIGNAAQIFPELVMRGVVPDVVTDQTPAHDIMSYVPAGMSVQ
                     EADALRKADPKRYAERSQESMARHVEAMLAFQKKGAEVFDYGNNLRQRAYDWGVRQAF
                     DFPGFVPAYIRPLFCEGKGPFRWVALSGDPEDIYRTDEAIAELFPEDLHLQRWIRMAR
                     EKVPFQGLPSRICWLGYGERARAGLRFNELVAQGVISAPIVIGRDHLDAGSVASPNRE
                     TEAMLDGTDAVSDWPILNALINAVNGATWVSFHHGGGVGIGFSQHAGQVIVADGTPEA
                     AVRLQRVLTADPGMGVVRHADAGYPQAIQFARAHGIQIPMLPEE"
     gene            7345..7731
                     /locus_tag="PKL60_05180"
     CDS             7345..7731
                     /locus_tag="PKL60_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95564.1"
                     /translation="MQKKGLRIFFFVLAILLGVGVGLAFGWLFMPAAAPKNASLEELR
                     ADYKTDLTLMVAESYAQNQDLPAALDSLAQIQPDDPYSLLVNTVNYARGVGYQESDLK
                     LIQALIEAIDPAAYREWLGGRGTDGN"
     gene            7721..8074
                     /locus_tag="PKL60_05185"
     CDS             7721..8074
                     /locus_tag="PKL60_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95565.1"
                     /translation="METDNHSHLYLITGLILGAALGLLISLVIAPAVHQVALPQELSP
                     QAKAGYRLEIARAYQAAPNPERALSRLALLNDPDPAEALIAQAQELLASGGSEDSARA
                     LISLAGLLQSPPPVP"
     gene            complement(8147..9559)
                     /locus_tag="PKL60_05190"
     CDS             complement(8147..9559)
                     /locus_tag="PKL60_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNW95566.1"
                     /translation="MKQFLRKLKAALPEKRKTRIVLGVLVALGIIGALVLGSFTNNFV
                     AGMSILNLPGVPVLSENTPGTGQTPGAEGTPAVATLQPILETQEAWDGKSRVNVLIMG
                     LDSRDLEETAPRTDSMILFTMDPVNLTAGMISIPRDLWVKIHGADYGKINTAYSIGEA
                     YKLPGGGPALAAKTVEDLLGVPIQYYAQLDFEAFIKFVDHIDGVKINVPERIQLDVVG
                     TKLSMWLDPGVVTLPGQWALAYVRARGTAGGDFDRAQRQQQVILAIRDRVLDFNMMPT
                     LISKSGEIYADLSTGIRTNLAPDEVIKLGIKVLDIPRESIQQAVIDGKYVNFGRSPDG
                     LEILRPIPDKIRELRDEIFYGVESSGTGSAQDVQARLQDEQARVSILNSSSSAGLAEK
                     TGEYLKGQGLNIVGQSDSGYQVYTRVTLYGSKPYTLKYLVDFLKISSPGSIIYAYDPN
                     ATVDIVVELGDDWAGANALP"
     gene            complement(9617..9838)
                     /locus_tag="PKL60_05195"
     CDS             complement(9617..9838)
                     /locus_tag="PKL60_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95567.1"
                     /translation="MIDQEFLNLLRCPVCVKEQKGTLELYRESWLICPDCGRKYPIWD
                     DIPVMLIDEGDKWAKTEKEALPVPPPQEG"
     gene            complement(9835..10071)
                     /locus_tag="PKL60_05200"
     CDS             complement(9835..10071)
                     /locus_tag="PKL60_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95568.1"
                     /translation="MSEQIVQRNEQVIKTEVKELVQVVNDDFRAPYLIQDFFANFPGA
                     LTRGQSPPYGKMDASKQPGAMKLLRERTKKPEDL"
     gene            complement(10505..12334)
                     /locus_tag="PKL60_05205"
     CDS             complement(10505..12334)
                     /locus_tag="PKL60_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNW95569.1"
                     /translation="METHGKRTQYKTETQVLFILLAGVLLAAMIAAALIFAYETRHAD
                     TIYPGVSVGSVDVSDLTTGQAVLKLKEQNDYSTQGQLLFAFDEMRWLYTPAQLGYALN
                     PYVSAEKAFAVGRDASLVRSLAAQLNSARQGVRIGPTATYDQSRAYAVLQEIASQVDK
                     PVVEAQITLNGTEVQVTAGQVGRSVDITATLALLDDFLLHERDGVIPLVVTEQQPEIM
                     DAQAAADLASEILSQAFSLQMPAGYANLGPWVIEPADLARLILIERGDDGAGAEYKIA
                     VNQQAINSYLVRLAPAVRTEPVNARFIFNDDTRQLELIQSAVVGQALNLEASVQTVLD
                     ALQSGAHSAQLVMERVDPQVKDDATAESLGITELVIAETSYFYGSDDARIQNISAAAS
                     SFHGLLIPPGATFSMASQLTDISLENGYAEALIIVGGETVKGVGGGVCQVSTTLFRTA
                     FFGGFPIVERHAHAYRVGYYEQRSNGGWDTDLAGLDATVYVPLVDLKFVNDTPYWLLM
                     ETYLRGYSLTWKFYSTSDGRSVEWDTTGVTNVQPPPEPLYRENPELPAGTIKQVDWAV
                     EGASVYVTRNVYRGGALYFNDSFETNYIPWQAIYEYGPGTDVP"
     gene            12504..13085
                     /locus_tag="PKL60_05210"
     CDS             12504..13085
                     /locus_tag="PKL60_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNW95570.1"
                     /translation="MRKQFRFLALLLIAGLVGGTFLALPANRLALAADSSAQAGQEAA
                     TATPGVPGFITSTPGADGSIKHIVRSGEFLITIAQAYGISLDELLALNNLTAESILQV
                     DQELIIRKATNPLPALTSGTVIAPTATLRPSLTPQPSRTPALTAVPTPTPTRGPGVFT
                     RVFSGRAAYVGIGIVVLILLGAALLIISSRRIH"
BASE COUNT         2615 a         4049 c         3880 g         2737 t
ORIGIN      
        1 gcaagcgcct cttaagcgtt aggttctggg ttcgagtccc agcagggtca tttttttatt
       61 gaacttcccc cagcattacg tccccacttc ccaaggagct gcgcaagcga gtttagctgt
      121 ccaaccctgc ggttgtcttt ccacgaactt ccagacctta tagatgcgct tgtccggata
      181 gcagcccatc gggggaatga cctgctcttc gcgctgggtg cggaaggcgc gcaagaccgc
      241 cttgctgaag cgccacaggc tggcggtgga gcggctgccc ttgtggatga tgaacactgg
      301 aaactggtgg tccaccatcc gctggcgttg ctccttggtg agctcaagcc acaagctgta
      361 ttccaccaca taggccttgg tctcgttaaa gctgccgcac tgcagcagag tgttctcgtg
      421 cgaaatgaaa ttcatcgtcg aaagcgcgct ccgcatcccg cctgagaaag agggtctgcg
      481 ggattttcta acgcctgtcg tggatttgca ccagaaaatt gccggtcaac cacaccaatc
      541 cgggattgtt gtgaaagggc cgcgcggcgc gccaatgcgt gctcatccag agaataatcc
      601 tccgaatgca gggagtggat aagctctccg ctggcgacct cgctgtcggt attcagcgcg
      661 gtcgccgcgc ctttcgcttc ggattggata cagcggatcg ggtagcgccc ggcgttgcgc
      721 gccgtatagt cgcgaaggat tgcgggtatg cggtcgctgg agtaggaagc gttgatgatg
      781 cgcgcgaggt tcccagagct ttgccgctcc accgaatcca atgccttgct caggtacttt
      841 tcgctgttgt aagtacaggg gacaatgctg attttcggga cgcacatttc catagggcgg
      901 gctctccaat caagcttgga tatgaccgtt attcgcgtgt ttgcgccgct cttttttttc
      961 ggcgtttcca ctatacgcta taattctgct gtcagtcaga tttgctgaaa gaaccaccgc
     1021 aatgagagcc tcacgcctgc ttcttttgcc gctgttgccc gtacttgtgt tcacgggctg
     1081 cgctcccagc ccgggtgcgc ctacccgcac aactccaagc gaaaccgctc ccacccgggc
     1141 agcgctccca accacagcgc tcaatacccc gccagcgccg gaggggatgg tcttcatccc
     1201 ggccggcgaa ttccagatgg gctgtgaccc ggcgagaaac agcggttatt cctgcccggc
     1261 ggatgaactt cctatccaca ccctgacgct gaaagctttt tttatcgacc gctatgaggt
     1321 gaccaacgca ggctataacg attgcgtgct tgcgggcggc tgccggcccc cctctgtcct
     1381 ttcgtccgaa acacgcgagc attgttatga catttccgtc ttcggggatt tcccggtgat
     1441 taacgtgagc tggaaggacg ccgacgcgta ctgccgttgg gcgggcaaac gcctgccgac
     1501 cgaagcggaa tgggagaagg cagcccgcgg cggccagaac gcggcttttc cctggggcaa
     1561 tgaccagccc agctgcatcc tggcgaacgc cttcgacccg ctttccgggc acttatgcac
     1621 tggggatacc acaaaggtcg gtactcatcc ggacggcgca tccgctttcg gcgtggaaga
     1681 catggccggt aatgtgtggg agtgggtgag cgactggtac tcggagacct actacgctga
     1741 atcccccgcg atagaccccg gcggaccgca gggagacacc gtaaaggcgc tgcggggagg
     1801 cagttggggc agccgtccgc cttccctgcg cgcgtccggg cgcagctttg aactcagctt
     1861 ttacaacagc tcggatatcg gtttccgctg cgcgatggac agccaaccct gaaagcgcgg
     1921 aaatttccac ctgcccgccg cgggggcagt gtaacattct atccataacc cggattactg
     1981 aggagaatta tatgcccgcc gagaacaccc catcccagcc agacccagtc atccgcctgt
     2041 tcaagtcgga ctttctcgag ttcttcacgc atatcagccc ggtggctgtg ctggtggtgt
     2101 tcctgccgtt gattggcttt ttgctctggc gcgcggcaag cgttaactcc actgcctggt
     2161 ggaatgttcc gctcgctttc ctgattggcc tcgcggtttg gccgctggtg gaatatctgc
     2221 tgcaccgctt tgtgttccac ttcaagccgg ctaaccccag cgaacggatg cagcgctttc
     2281 ttttcctgat gcacggcgtg caccacgccc aaccgcgcga gaaaacccgc ctggtgatgc
     2341 cgccggtggt cagcctgccc atggcggcgc tgttttacgg gctgttctgg ctggtgattg
     2401 gcaagctgct gggcgcgccc ggttatgtgg acggcattgc ggggggcttt ctgttcggtt
     2461 acgttctcta cgatatgacc cactacgcgc tgcaccactt cagtctgaaa ggcgcttatt
     2521 tccgcaagct gcgccggcat catatggcgc atcacttcaa gacgcacgat gcgaaatatg
     2581 gggtctcgac ctggttttgg gacgatgtgt tcaagaccaa cccggcggag tgaaactact
     2641 ccgcctgtgc ggatctcgta tttcactcgc cacgaagccg gtagaccacc caattatcaa
     2701 tcatctcttc cggctcgtag aaagacatta acagccgtac tttttccttt atctgggcgt
     2761 tttccccggg gaagtccaga aaagcccagc cctccagctt cgtgtcaatc atcagcagtg
     2821 gtttttcatc caggagctca tccaggaatt ccagcgcgcg cgcctgcccg gatgcgtccg
     2881 cgtccatcag cgggcgcatc accaggtagc gcgtagggct ttcgcgctgg gcaaagtagt
     2941 tcacgcgcgt gtcccgccag accaagacgc tatcctccgg gctggtgttc tcacggacgt
     3001 agcgaatggc agcctggcgg gcggtcaggc tttgctggta ctcccccgcc cgctcggctg
     3061 tctccagccc gaagcccaac accagcagcg cggctgccaa ggcgccaact gccccgcgcg
     3121 aaatcttcct gccgcctgag agcgccagcc actgatccaa caccgaaaaa gccgccgcac
     3181 tgaaaagcgc cagcgcggga agaagcgtct caaagtaata cttactcacg tcgccggtca
     3241 tcagaattaa gcccagctcc agcgctccgt ccagcagcat cagcgcgaac aggctgcgtt
     3301 tgagcggctc ctgttttttg cgcgggaaga aagcccccgc cagcacgccc aacagcgcga
     3361 tggggaaaat gggcaagtgc gcgatgtagc gcaggtcaac cgcgttgcgc agccggtccg
     3421 ccgggctttg ggcagccgag gcggaaaagt ggaagttata agcaaaggcg gcatcccagg
     3481 cttctgccag cgcccctttg ctgaggaaat acaatcccgc cagcagcacg actgccagca
     3541 cgccggccgc catccagccc agcttgcgcc aggctgcggg cttgcgccgc agcgcccagg
     3601 ccagcagcaa aatgccgagc gccagccaaa tacccacagc cgtttgcttg atcaggaacg
     3661 ccagcccgcc caggattccg atgagaaggt aggcgcgttc gggttttgcg cggctttcgg
     3721 cttgcgtgaa gagcagcagc gcgccaaact gcagcggcag ggtccaatca gtggtctgat
     3781 tgccacccaa aaagatgaaa aacagcgcct gaagccacaa gacggtgccc agcgcggctg
     3841 ccagcgagcc aaaatgggct ttgatgagct tataacccag atacgccgcg agcatcagcg
     3901 ccaaaagctc caacgcccag acgccccaat gcgagccggg cgaaagcgcc gcgcccaggg
     3961 cgttgatgta gaagaccagc gggggcttgt ggtcccaaag ctgcaggtag ggcaggtcgc
     4021 cctgcaggat gcgccagccg gcatagaggt aaacaccgga gttatcgttg acgccgggcg
     4081 caaaggcagg cgccaccggc agcatgaccg ccagcgcgcc cgccagcggg atgagcagtt
     4141 ccagcagtct attcggcgct gggatagggg atgctttggt ctgctttggt ttcgcgctca
     4201 ttcctgtgcc gccttccacg gctgtactaa gcctgcattg ctgaaaaacc gattacaatg
     4261 attttacaat gttcgcaagc gcataacaat agctctcggc gccgcgcccg ccccggcgcc
     4321 gcgcaacaac tcgtgtttcg caaaggagac cagatgcagc tcacccacac cgccctctac
     4381 gtccgcgacc tggaagcgat gaaggacttt tacatccgct gtttcgacgc gcaagccagc
     4441 caaaaatacc acaaccgtaa aaccggtctg caaacctact tcctcagttt cggcgcgggg
     4501 gcaaggctgg agctgatgac ccgcccggac ctgagcgaac gggatggggc tgcccagacc
     4561 tatggatacg ctcacatcgc ttttcaagtc ggctcgcgcg aggccgtgga cgagctgacc
     4621 tgcacgctgg ccggggttgg ctgcctggtg ctcagccgtc cgcgcatcac cggcgacggg
     4681 tattacgagg ccagcctgca ggacccggaa ggcaacctgt tggaattggt cgcctgagga
     4741 atagaggcgg agatgcgctc agaagggacc atgttctcgt taattacgga ctgcgcgctg
     4801 aaagacccgc gcgtgcggct ggtcctgctc tccggctcgc gcgtcaatcc cgctgccccg
     4861 cgcgacgacc tgcaggattt tgacatcgta tatttcgtgg ccgacctgga ttcttaccgc
     4921 gcaagcgaag cctggctgga cacatttggc agccgcatga tgatgcaaaa gccggaggag
     4981 atgggcgcga acccgcccga aaagccggac ctggcgtacc tgatgctctt tgaagacgga
     5041 aaccgcatcg agctcagcct ccgcccgctc tcccgaatac gggaaaccgc cgaaggcgaa
     5101 ggtttctggc gcgtgctgct ggataaagac cagttggccg ccagcctgcc caagccaacg
     5161 gccgcgcagt ttcaaatcca gccgcccagc gccaaaactt ttgaggactg ctgcaacgaa
     5221 ttctggtggg tcagccccta cgtggccaag ggcctgtgcc ggcaggagca gctctacgcc
     5281 gtctggcaca tggaagccgc catccgcaag gagctgctca aaatgcttgc ctggctggta
     5341 ggattgcgcg agggctggca ggtgcagccg ggcaagcagt acaagtactt gcaggcgcat
     5401 ctggacccct ccgattggaa gctgttgacg accacctgcc gcctggacaa acccgccaac
     5461 tgttggcagg cgctttttgc cgcgcaaagg ctcttccggc acgccagccg gcaagtcggg
     5521 gagcagttcg gctttgccta tcccccgtac gacggcaagc taagccccta tatcgaggcg
     5581 ttataccagc gctggcagga tttttccagc cgaccgcgct aatcggctga tgtataatca
     5641 ctttatcatt gtgtttcagg aggtctcacc cgatgtctga accaagaacc gtgcgcgccc
     5701 caagaggcac gcagctcagc tgcaagaact ggcttatcga agcggcatac cgcatgctcc
     5761 agaacaatct ggacccggag gtggcggaaa aacccgcgga actggttgtc tacggagggc
     5821 gcggtcaggc cgcccgctcc tgggaggctt ttgacgcgat tttgcgttcc ctgcgcgcgc
     5881 tggaaccgga tgaaactctg ctggtgcagt ccggcaagcc ggtggcggtg ttcaagtccc
     5941 acccggacgc gccgcgcgtg ttgattgcca actcgaatct ggtgccgcac tgggccacgc
     6001 aggaacattt tgacgcgctg gcggccaaag ggctgatcat gtacggtcag atgacggccg
     6061 gctcctggat ttacatcggc acgcagggca tcctgcaggg cacctacgaa acgctggggt
     6121 cgctcgcgaa acaaaaaggc tggccgtcgt taaaagggaa atttgtgctg accgccggct
     6181 tgggcggcat gggcggcgcg cagccgctgg ccgtcaccat gaacgaaggc gtcgggctga
     6241 tcgtggaagt cgaccgcgag cgcgcgcagc gcaggctgga aattggctac gtggacgcac
     6301 tgacggacga cctggaagac gccatgacca tggtggaaga ggcggtaaag ctgggcatcc
     6361 ccaaatccat cggtctgatt ggcaacgcgg cccaaatctt ccccgagctg gtaatgcgcg
     6421 gcgtcgtccc ggatgtggta accgaccaga ctccggcgca cgacatcatg agctacgttc
     6481 ccgccggcat gagcgtccag gaagctgacg cgctgcgcaa agccgacccc aaacgctacg
     6541 ccgagcgctc ccaggaatct atggcgcgcc acgtggaagc tatgctggct ttccagaaaa
     6601 aaggcgcgga agtttttgac tacggcaaca acctgcgcca gcgcgcctac gactggggcg
     6661 tcaggcaggc ttttgacttc ccgggtttcg tgcccgccta tatccgtccg ctcttctgcg
     6721 aggggaaagg acccttccgc tgggtggcgc tctccggcga cccggaggat atctaccgca
     6781 ccgacgaggc tatcgcggag cttttcccgg aagacctgca cctgcagcgc tggattcgca
     6841 tggcgcgcga aaaagtaccc ttccaggggc tgccctcccg catttgctgg ctgggctacg
     6901 gcgaacgcgc ccgcgccggc ctgcgcttta acgaactggt ggcgcagggc gtcatcagcg
     6961 cgccaatcgt catcgggcgc gaccatctgg atgccggctc ggtagcctcc ccgaaccgcg
     7021 aaaccgaagc tatgctggac ggcaccgacg cggtgagcga ctggccaatt ctgaacgcgc
     7081 tgataaacgc ggtgaacggc gccacctggg tctccttcca ccacggcggc ggcgtgggca
     7141 tcggcttcag ccagcatgcc gggcaggtta tcgtcgcgga cggcacgccc gaggcagccg
     7201 tccgcctgca gcgcgtgctc accgccgacc caggcatggg cgtggtgcgc cacgcggatg
     7261 cgggttaccc tcaagcgatt caattcgcgc gcgcgcacgg catacaaatc ccgatgctgc
     7321 cggaagaata agggcacaac gctcatgcag aaaaaagggc tgcgcatttt cttcttcgtt
     7381 ctggcaattt tgctcggggt cggtgtgggc ctggcattcg gctggctgtt tatgccggct
     7441 gccgcgccca aaaacgccag cctggaagag ctgcgcgcgg attacaaaac cgacctgacc
     7501 cttatggtcg ctgaaagcta cgcgcaaaac caggacctgc ccgccgcgct ggacagcctc
     7561 gcgcaaatcc agccagacga cccctactcg ctgctggtga acaccgtgaa ttacgcccgg
     7621 ggcgtcggtt accaggaaag cgacctgaag ctcatccagg cgctcatcga agcgatcgac
     7681 cccgcggcgt atcgggaatg gttgggaggg cgcggcactg atggaaactg acaaccactc
     7741 ccacctgtat ctgattaccg ggcttatcct cggcgcggca ttgggcttgc tcatctcatt
     7801 ggtcatcgct cccgcggtgc accaggtcgc gctgccgcag gagctcagcc cgcaggccaa
     7861 agccgggtac cggctcgaga ttgcgcgcgc gtatcaagcc gcgccaaacc cggaacgcgc
     7921 gctcagccgg ctcgcgctgt tgaatgaccc cgaccccgcc gaagcgctta tcgcgcaagc
     7981 gcaggaactg ctggccagcg gcggctcgga ggattccgcc cgcgcgctga tttctttggc
     8041 cggtttgctg cagagcccgc cgcccgtccc ataagccttg aagctttcct tcgaataaaa
     8101 aagctgacga tgtgtcagct ttttttgcgt ttctgttgtg aagcggtcag ggcagggcgt
     8161 tcgcgccagc ccaatcgtcg cccagttcca cgacaatatc cacggtggca ttcgggtcgt
     8221 aggcgtaaat gatgctgccg ggggagctaa tcttgaggaa atccaccagg tatttgagcg
     8281 tgtacggttt gctgccgtag agcgtgacgc gcgtgtagac ctgatagcct gagtcgctct
     8341 gtccaacgat attcaagccc tggcctttca ggtattctcc ggtcttttcc gccaggccgg
     8401 cgcttgaaga ggagttcagg atggatacgc gggcctgctc atcctgcaag cgcgcctgaa
     8461 cgtcctgagc ggaacccgtt ccgctgcttt ccacaccgta gaagatttca tcgcgcagtt
     8521 cgcggatttt gtccggaatt gggcgcagaa tttccaagcc gtcgggcgag cgcccgaagt
     8581 tcacgtattt gccgtcgatc accgcctgct ggattgactc gcgcgggata tccaacacct
     8641 tgatccccag tttaatgacc tcatccgggg ccaggttggt gcggatgccc gtcgaaaggt
     8701 cggcgtaaat ttctccggac ttggaaatca gggtcggcat catgttgaaa tccaggacgc
     8761 ggtcgcggat tgccaggata acctgctgct gacgctgggc gcggtcaaaa tccccgccgg
     8821 cagtgccgcg ggcgcgcacg taagccagcg cccattgccc gggcagcgtc accacgccgg
     8881 gatccaacca catagacagc ttggtgccga ctacatccag ctgaatgcgt tcggggacat
     8941 tgattttcac gccgtcgata tggtcaacga acttgatgaa tgcttcgaaa tccagttgag
     9001 cgtaatactg aatcggtaca cccagcaaat cttccacggt cttagccgcc agcgcgggcc
     9061 caccgcccgg cagcttgtaa gcctcgccga tgctgtaggc ggtgttgatc ttgccgtaat
     9121 ctgcgccgtg aattttcacc cataggtcgc gcgggatgga aatcatgccc gccgtgaggt
     9181 tcaccgggtc catggtaaac aaaatcatgg aatcggtgcg cggggcggtc tcttccaggt
     9241 ctctggagtc caggcccata atgagcacat tcacgcgcga ttttccgtcc caggcttcct
     9301 gggtctccaa aatcggctgc agggtagcaa cagccggcgt tccttccgcg cctggggttt
     9361 ggcctgtgcc cggagtgttt tcggaaagca cgggcacgcc cggcaggttc agaatgctca
     9421 tgcctgccac aaaattattc gtaaagctgc ccaacacaag cgcgccgatg atacccagcg
     9481 cgaccagtac gcccagaacg atgcgcgttt tgcgtttttc aggcagcgct gccttcagtt
     9541 ttcgtaaaaa ttgtttcata agagccttca aataaagcgc ctggtgactg accccaggcg
     9601 tttgaaatcg tgttccttaa ccttcctgcg ggggcggcac cggcagggct tctttttcgg
     9661 tcttcgccca cttgtcgccc tcgtcaatca gcatgaccgg aatgtcatcc cagatggggt
     9721 acttccggcc gcaatcaggg caaatcagcc agctttcgcg gtaaagttcc agcgtgcctt
     9781 tctgttcttt tacgcacaca gggcagcgca gcaagttcaa aaattcttgg tcgatcacag
     9841 gtcctccggt ttcttggtcc gttcccggag cagtttcatc gctccgggct gtttggacgc
     9901 atccatttta ccataaggcg ggctttgccc gcgcgtcaga gctccaggga aatttgcaaa
     9961 aaaatcctga ataagatacg gagctctgaa gtcatcattc actacctgta ccagttcctt
    10021 tacttcagtc tttattactt gttcgtttcg ctgaacaatc tgttctgaca tggttctttc
    10081 tcctttggtg ttttggtgat aaccaaatct taccaagcga ttgaggacca tgtccttttg
    10141 taaatgtgcg aaatttatgt taccttatcc caggttttgt tacaaaggat tgaccgtacc
    10201 ctgacacgca agggattatc agccttgctc ttcttcctcc ccttcccagc ccatgaaatt
    10261 tatccattat cctaattgtc gttgggacgg ggagttgtct gggattctat caaacgtttt
    10321 ccctgctcca gcggcttggc cggggaaaat agagctgctt gctgctggag aaggctcagg
    10381 gttagggcaa ccatgaaaca tgatagaaaa gaagaaatca ggggattcga ttcgaggaaa
    10441 acccgccttg acgatttcat ccgctcatca ggatgtttct atttagcctt gacgggtctg
    10501 agctctaggg cacgtcggtg ccggggccgt attcgtagat agcctgccag gggatgtagt
    10561 tcgtctcaaa gctgtcattg aagtacagcg cgccgccccg atagacattg cgcgtcacat
    10621 agaccgaagc gccttccacc gcccagtcca cctgcttaat tgtgccggcg ggcagttcgg
    10681 ggttctcccg gtaaagcggc tcgggcgggg gctgcacgtt ggtcacgcca gtggtatccc
    10741 attccacgct gcgcccgtcg ctggtggagt agaacttcca ggtcagggaa tagccgcgca
    10801 gataggtttc catcagcaac caataaggcg tgtcattgac gaacttaagg tccaccagcg
    10861 gcacgtagac ggtcgcgtcc aggccggcca ggtcggtatc ccacccgccg ttggagcgct
    10921 gctcatagta gcccacgcgg taggcgtgcg cgtgcctctc cacaatgggg aagccgccaa
    10981 agaaggccgt gcgaaagagc gtggtgctta cctgacacac gccgccgcca acgcctttca
    11041 ccgtttcgcc gccgacaata atcagggctt ccgcgtagcc gttttccagg ctgatatccg
    11101 tcagctggct ggccatcgag aaggtggcgc cgggcgggat gagcagcccg tgaaaactgc
    11161 tggcagccgc gctaatgttc tggatgcgag cgtcgtccga gccgtaaaag taggatgttt
    11221 cggcaatcac cagctcggtg atacccaggc tttccgccgt cgcgtcgtcc ttcacctgcg
    11281 ggtccacgcg ctccatcacc agttgggcgc tgtgcgcgcc gctttgcaag gcgtccagca
    11341 cggtttgtac gctggcttcc aggtttaagg cttgcccgac gaccgcggat tggataagtt
    11401 ccagctggcg ggtgtcgtca ttgaaaatga agcgcgcgtt caccggttcg gtgcgcaccg
    11461 cgggcgccag cctgaccagg tagctgttaa tcgcctgctg gttgaccgcg attttgtatt
    11521 ccgcgcccgc gccgtcatcg ccgcgctcga tcaggataag cctggccaaa tctgccggct
    11581 cgattaccca gggtcccagg ttagcatatc ctgcgggcat ctgcagcgag aacgcttggg
    11641 aaagaatttc actggcaagg tcggccgccg cctgggcatc cataatctcc ggctgctgtt
    11701 cggtcacgac cagagggatg acgccgtcgc gttcatgcag caaaaaatcg tccagtaagg
    11761 ccaaggtggc ggtgatatcc acgctgcgcc cgacctgccc ggctgtcacc tgcacttccg
    11821 tgccattgag cgtgatttgc gcttccacga ccggtttatc cacctggctg gctatctcct
    11881 gcaagacagc gtaggcccgg ctttggtcgt aagtggcggt ggggccgatg cggacgccct
    11941 ggcgggctga attcagttgg gctgccaggc tgcgcaccag gctcgcgtcc cgccctacgg
    12001 caaaggcttt ctccgcgctg acataggggt tcaacgcgta accaagctga gcgggtgtat
    12061 agagccagcg catttcatcg aaggcgaata acagttgccc ttgggtggaa tagtcgttct
    12121 gttccttcag cttcagcaca gcctgcccgg tcgtcaaatc cgagacgtcc acgcttccaa
    12181 cgctgacgcc cgggtagatc gtatcggcat gcctggtttc gtaggcaaat ataagcgccg
    12241 cagcgatcat cgcagccagc agcacgcccg ccagcaggat gaagagcacc tgagtttctg
    12301 ttttgtattg tgtgcgtttt ccgtgcgttt ccatatgctg tgattataac gacttccctc
    12361 gctgccgcag tatcttgaag agtccgggta caaggttgat tttttgctct ccttcaggac
    12421 ggaaagggcg cacttttgtt ccccaacaga acaggtttcc ggcggcaagg gtataatcag
    12481 ggtgctttat tatcctggaa cacatgcgga aacaattcag attcttggcg ctgctgctga
    12541 tcgcgggttt ggtcggaggg actttcttag ccctgcctgc aaaccgtttg gcgttggcgg
    12601 ctgattccag cgcccaagcc gggcaggaag ctgccacagc tacgcccggc gtgccgggat
    12661 ttatcacttc caccccgggc gcggacggca gtatcaagca tattgtcaga tcgggggaat
    12721 tcctgattac catcgcgcaa gcctacggca tcagcctgga tgagctgctt gcgctgaaca
    12781 acctcaccgc cgagagcatc ctgcaggtcg atcaggaatt gattatccgc aaggctacca
    12841 acccgctgcc cgcgctgacc agcggtaccg taatcgcccc cactgccacg ctgcgcccct
    12901 cgctcacccc gcagcccagc cgcacgcccg cgctgactgc cgtgcccact ccaaccccta
    12961 cacgcggacc cggcgtgttc acgcgcgttt tttcaggtcg ggcggcatac gttggcatcg
    13021 gcattgtcgt attgattctg ttgggcgcgg cgctgctgat tatcagctcc aggcgaatcc
    13081 actaaacgca gaatccacgc gcttgtcacc ctgaacgcct gtagtgggtt gggtggagcg
    13141 gcaccaagcc ttcatcagca atttcctttt cggagcgcaa cccaacacaa ttcgcttggt
    13201 gaagtagtag gttgggtgga gcgactccgg gcttgcatca gcaatttcct tttcggagcg
    13261 caacccaaca caattcgcct g
//
LOCUS       DAOQAD010000047        13241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_5162_length_13241_cov_5.370620, whole genome shotgun sequence.
ACCESSION   DAOQAD010000047 DAOQAD010000000
VERSION     DAOQAD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13241
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5162_length_13241_cov_5.370620"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PKL60_05215"
     CDS             <1..1096
                     /locus_tag="PKL60_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW95571.1"
                     /translation="QEDIRFYQQLAYRASAVLSAKEQYGRLQNEKTALSAALDSLPEP
                     VMVLDNAGSVVLGNAAARQLMETNGQHGTKPVPFSLAFPEAGAEPANGISAKPFVVTA
                     GEREFEVESLPLHPTGGMRGRLLIFNDTSSQKRLNAQKNEFVTNISHDLRSPLSQVRG
                     YLSLLENIGNLSEEQVKFIDRIRLSIENMSRLVSKVLSLEMLDSDGGVKFTSFDLKEM
                     IEEVLTLLELQATQRKVSIKTNFTGLRNPRFTADRLLLHQAVFNLVENAIKYSPIGGE
                     VLVSAAKDSAKLSLTVQDHGKGIAPLDQPKLFTRFFHVEEADSIEGSSRGLGLAIVKS
                     IADKHGGTIRVQSQLGEGSTFTLEIPVRKG"
     gene            1286..5638
                     /gene="rpoC"
                     /locus_tag="PKL60_05220"
     CDS             1286..5638
                     /gene="rpoC"
                     /locus_tag="PKL60_05220"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNW95572.1"
                     /translation="MKALRINLASPEKIRGWSYGEVLKPETINYRRLRPEKDGLFCEA
                     IFGPTRDYQCYCGKYKNKKYQNVVCDKCGVEVTKASVRRERMGHIELAAPVAHIWYTR
                     RVPSHLGLLLDISRRNLDRVLYFAQYIVTYVDEEARQKALKRLEDEISLSERELIEKV
                     NGQIAEAKKQRDRQLRELDGRVEAIENEYDEQVSSLFEPVIAEGQRVERELTEKVGKT
                     IHKPIVFSLENFEKVLVEANEEVASKHISRVQRIVKDRMEDLEKDLRNEKQIKLEELK
                     LQKEAIRAEVDLIMEGIRNQLEDQEAVTKTENAKLRDELYELQPFTFLSEARYRELKS
                     RWGQVFKADMGAEAFHDILHRLDLNALAEDLWQEVRTTRSKQKRKKATSRLKVVEAFR
                     RSSNLPEWMILTVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELG
                     APDVIVRNEKRMLQEAVDSLIDNSQRGRMLSRRGRRELRSLSDMLKGKKGRFRRNLLG
                     KRVDYSGRSVIVVGPNLKLYQCGLPKTMALELYRPFVIAGLVEEHHYAHNLKGAKKFI
                     ERNRPEVWEVLEEVIQRRPVLLNRAPTLHRLGIQAFEPILIEGSAIQLHPLVTTAFNA
                     DFDGDQMAVHLPLSDKAVSEARQYMLASKNLLKPASGEPIISPGKDMVLGVYYLTMLA
                     NRPKQGDGRAFANIDEVILAYQLGQVELHTRIKLVVESWYSDSDKPERLSEAEKRLQE
                     TTVGRAIFNDILPDKIRFINRTLDKGGIRDLIAEIHELLNDEETTDVADKIKDIGFRY
                     ATQSGSTLAVADIEVPPEKADIIREALLKVETAQKAYRRGLQTEHEQNQNFIKIWQDA
                     TEEVGKAVKAHLDPNGNLATMANSGATKGGFSPISQLAGMRGLMADPSGRIIPMPIRS
                     NFREGLTALEYFISTHGARKGLADTALRTADAGYLTRRLVDVAQDMIINNEDCGTEEG
                     IYINAKDDVAGQSFGERIYGRLLAESVVHPKTGEVLAGRNELIDYKLQRVILQAGVQT
                     IKVRSPLTCELLHGVCARCYGMDLARGKMVNLGAAVGIVAAQSIGEPGTQLTLRTFHT
                     GGVAAAGDITTGLPRVEELFEARVMPKGEAVISRIKGVVHLIQSPKNPDQRIIRVDHS
                     EMTSDTYEIPEGWQVQVQDEGEVSIGDVLASMEDATIGAQHAGRVRIEDNNVIVSYEN
                     RETEEYDIPPTARLTVKEGQIVEGGEPLTEGSLNPHTILHIKGREATELYLMTEIQKV
                     YRTQGQNINDKHFEVIIRKMLNKVQITRPGDSQYLPQDLVDRLELRKVNEDLIAKGLR
                     PARFINVLLGVSKASLNTDSFLSASSFQHTIKVLAGAAIASKEDLLYGLKENVIIGKL
                     IPAGTGFDPERFAGQNVEPQPGKEDIQVKQMNIFMEDDSSVLALNEEEDSAADDYSDA
                     DDDPELDDLREDEE"
     gene            5751..8198
                     /gene="gyrA"
                     /locus_tag="PKL60_05225"
     CDS             5751..8198
                     /gene="gyrA"
                     /locus_tag="PKL60_05225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNW95573.1"
                     /translation="MDSTSIKKININTEMQQSYLDYAMSVIVSRALPDARDGLKPVQR
                     RILYAMYDMGVRPDQPHRKSARIVGEVLGKYHPHGDSAVYEAMARMAQDFSERYPLVD
                     GQGNFGSIDGDPPAAMRYTEARLSEAAVEILKQFDMDTVEFTENFDGSLQEPLVLPSA
                     LPNMLVNGASGIAVGMATNIPPHNLGEVIDALTLLLENWDKIDDISTEEIMRFIKGPD
                     FPTGGLILQEETTETLAQIYGSGNGVIQIRARTRLEEMSRGRMRIIVTELPYMVNKSA
                     LIERIAELVRDGVVDGITDLRDESDRQGMRIVIDLNKNADPEKVLSVLYKKTAMHSTF
                     AINILALVGGSPHRLSLKQSLRVFAEHRLEVIRRRSEYELRKNEERRHILEALRVAIK
                     NIDLVIQIIRKSKSAEEARQNLMSKLSLDEIQATAILDLQLRRLASLERQKIEDEYQQ
                     VSEAIKNLKALLKSPQKMRQVEIDELKLLKSKYADPRRTQIIRLGEGEKVSELLTQTD
                     VLPLENFWIEASADGVLSRTEGDKPNRLGGENAPWSMLRANSHQTVFLVSSQGKAAAI
                     HSLAIPVQKNGEQGIAAHKLSPLNGNDVLQGIFSVPMDRDKLPETYVLTVSRQGMVKR
                     SALQELPGASANSFVLTKINEGDELFQVLLTNGKQDILMTTANGMSIRFHESEIRPMG
                     LIAAGVNGMKLKEGDYIVGVSLVDNKDSVALLTRKGLAKRVSAEDYPLQGRYGQGVIS
                     WKLAKDDQIVVQTAGRLSDKVICHFRKSASKVFTITSAVERTRAANGQGVFTLKAGDE
                     LIGATPLDDLSAYWEKA"
     gene            complement(8199..8774)
                     /locus_tag="PKL60_05230"
     CDS             complement(8199..8774)
                     /locus_tag="PKL60_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNW95574.1"
                     /translation="MNRDEALQFIRQYVKSESLIRHMLSVEAAMRFYARKLGENEEEW
                     AMAGLLHDFDWEIHPNAEEHPIAGAPLLREAGVSEDIIAAVLSHADHTGLPRDTTLKK
                     ALFACDEITGLITAVALVRPSRSLFDLEASSVRKKWKDKAFAAGTDRTMMELGAQEFG
                     VDLWEHVSNVISAMRAIAPDLDLVGSFRQAS"
     gene            complement(8786..9421)
                     /locus_tag="PKL60_05235"
     CDS             complement(8786..9421)
                     /locus_tag="PKL60_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNW95575.1"
                     /translation="MRRFLQVLVVCFCAGFILVGLLRLGMIFYAASKTVPISEVIPVP
                     VVIVPGAGLSADGTPSVPLRDRVQAAVDLYLAGKAQKILMSGDNSTEYYNEPESMRQY
                     ALSLGVPDEDIVLDYAGRRTYDTCYRAREIFGIQTAVITTQPYHLPRAIFLCDRLGIN
                     IQGVEVEQSRYVRTRYIFWQVREVFATLSAVWDIYIAKPLPILGQPEPIFP"
     gene            complement(9442..10494)
                     /locus_tag="PKL60_05240"
     CDS             complement(9442..10494)
                     /locus_tag="PKL60_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNW95576.1"
                     /translation="MSEYRKRYSFFLLVLCVLVSACAAQPAARPSALPAGVTPTAFLS
                     TSTPMPTFTPTSAPLGSPQNPIVMAVISAEADSVQISRLDSLSAQLSGFLGLSVAGKT
                     FPDYLSLEEALQKQEVQLAWLNPVEYLLASAKGLLNSQLVANHLGVTAYGVQFLGHKD
                     ANLTRYYDAGSNLATAPAATALAQFAGLTPCLTNEKSLPGYWVPLGYLAQNAVTTQSP
                     VLTRTYSAAIRALYIKGVCDFTATYAIASDPRTSSEVITDLPDVIDRLPIIWLSPAVI
                     PNLNFSVSPLLPLSVSAQITNYLLELSRTESGRQQLSELNAYEIARLEPQPDSAYDAL
                     RELLAVQNLRLTNLLP"
     gene            10581..11066
                     /locus_tag="PKL60_05245"
     CDS             10581..11066
                     /locus_tag="PKL60_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HNW95577.1"
                     /translation="MNMEVVQIQKPEGINFILGQSHFIKTVEDIHEALVTAVPGIRFG
                     LAFCEASHPRLVRWSGNDEIMLQLARENALKIGAGHCFILFLAEGFYPVNVLNTVKQV
                     PEVCRVFCATANPTEVIIAVTEQGRGILGVVDGQPPLGIEAEEDIAARKALLRRFGYK
                     L"
     gene            11083..11592
                     /locus_tag="PKL60_05250"
     CDS             11083..11592
                     /locus_tag="PKL60_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNW95578.1"
                     /translation="MDILTAIFTRRSVRRFTQEPVSDSDLETLLRAAMQAPSADNGQP
                     WHFVVLNERALMDQIPSIHPWSDMMKEAALGILVCGRIVEGEMGGYWQQDCAAAAENI
                     LLAAHGIGLGAVWLGICPEAQRMADFSALVGLPADVHPLALIAVGHPGRIAQAENRYD
                     PSKLHYNRW"
     gene            complement(11599..12198)
                     /gene="rdgB"
                     /locus_tag="PKL60_05255"
     CDS             complement(11599..12198)
                     /gene="rdgB"
                     /locus_tag="PKL60_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNW95579.1"
                     /translation="MKDLLLATTNPGKIQEITAILAGIPLRLLSPADFGLDISVVEDS
                     GSYTGNAHLKASAYARAAGIPALADDSGLEVEALGGEPGLRSHRYSPLTSATDRDRCL
                     YLLEKLKEAARPWKARFVCAVVVCGSSGEMLRAWGECPGEIIPEFRGENGFGYDPIFL
                     VAGSDKTMAELPNEQKNQLSHRARALQAALPALREFAGE"
     gene            12273..12764
                     /locus_tag="PKL60_05260"
     CDS             12273..12764
                     /locus_tag="PKL60_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNW95580.1"
                     /translation="MEKQHYSLRANVGFFFNKPIGFSRDFHAEFPEIYLEPDLNVLNL
                     AAVYRFSRTREGLLLQTELSGEIQAQCVRCLTESSVPVSTSFEELFYFPERTQEETDQ
                     LVPTDGYIDLADIFRDYLLLEIPINHICKPDCKGICVECGQNLNLADCSHTDSRILLT
                     DEE"
BASE COUNT         3165 a         3556 c         3763 g         2757 t
ORIGIN      
        1 gcaggaagat atccgcttct accaacagct ggcttaccgt gcttcggcag tgctgagcgc
       61 caaggagcag tacggccgcc ttcagaatga aaagactgct ctttccgccg cgctcgattc
      121 gcttcccgag cctgtcatgg tgttggataa cgccggcagc gtggttctgg gcaacgcggc
      181 agccaggcag ctgatggaga caaacggtca gcatggcaca aagccggtgc ctttcagcct
      241 cgcatttcct gaagccgggg ctgagcctgc caacggaatt agcgctaaac ctttcgtcgt
      301 cacagccggc gaacgggagt ttgaggttga gtcgctgcct ctccatccga ccggcggaat
      361 gcgcgggcgc ttgctgatct tcaatgacac ctcttcgcag aaacgcctca acgcgcaaaa
      421 gaacgagttt gtaaccaata tcagccacga tttgcgctcc ccactttcgc aagtacgcgg
      481 ctacctgagt ctgttggaga atatcgggaa cctgagcgag gaacaggtca agttcatcga
      541 ccgcatccga ttaagtattg aaaacatgtc cagattggta agcaaggtcc tcagcctgga
      601 gatgttagat tcagatggcg gggttaaatt caccagcttc gacctgaagg aaatgattga
      661 ggaagtcctg accctgctgg aattgcaggc aacgcagcga aaagtcagca tcaaaaccaa
      721 ctttaccggc ttaagaaacc cgcgtttcac cgcggatagg ctcctgctgc atcaggcggt
      781 tttcaatctg gtggagaacg cgattaagta cagccccatc gggggagagg tgctggtgag
      841 cgccgcgaag gacagcgcaa agctcagtct cacggttcag gaccacggca aaggcattgc
      901 ccccctggac caacccaagt tgtttacgcg cttcttccac gtcgaggagg cggacagcat
      961 cgaaggctcc agccggggtt tgggtcttgc gattgtgaaa tccatcgcgg acaagcatgg
     1021 tggtacaatc agggtacaaa gccaattggg agaaggcagc acatttactt tggaaatacc
     1081 tgtcaggaaa ggataaaggg gtggtagaag tctttagtat tggcgagaaa aacttgacag
     1141 cgacaactat tctttagtat aataataatt tgcatgaaat aacctaacac acgaaaaacg
     1201 cagaatctgg gaagaaaact ctcagtttct ttttcgtgtt gataccctcg ctcacgacat
     1261 ggaggctcgc gtggaaaata aacaaatgaa agcacttcgt atcaaccttg cttcccccga
     1321 aaaaattcgc ggatggtcct atggtgaagt tctgaaacct gagacgatta actaccgccg
     1381 tttgcgcccg gaaaaggacg gcttgttttg cgaagcgatt tttggcccca ccagggacta
     1441 tcagtgttac tgcggtaaat acaaaaacaa aaaataccaa aacgtcgtct gcgataaatg
     1501 cggcgttgaa gtgacaaaag cgagcgttcg gcgcgaacgc atgggtcaca ttgaacttgc
     1561 agcgcctgtc gcgcatatct ggtacacgcg cagagttccg tctcatctcg gcttgcttct
     1621 ggatatctcc cggcgcaatc tggatagagt cctgtacttc gcccaatata tcgtgaccta
     1681 tgtggatgaa gaggctcgcc agaaagctct gaagcggctc gaggacgaaa tcagcctgtc
     1741 cgaacgcgaa ttaattgaaa aagttaacgg gcagattgcc gaagctaaga aacagcgcga
     1801 ccgccaactg cgcgagctgg atggccgtgt ggaagccatc gaaaatgagt atgacgagca
     1861 ggtttccagc ttgtttgagc ctgtgattgc cgagggccag cgcgtggagc gcgaactgac
     1921 tgaaaaagtc ggcaagacca tccacaagcc gatcgtcttc tctctggaaa atttcgaaaa
     1981 agttttggtg gaagccaatg aggaagtcgc ctcaaagcat atttcccgcg tgcagagaat
     2041 cgtcaaggac cgcatggaag accttgagaa ggatttgcgg aatgagaagc aaatcaaact
     2101 tgaggaactg aaactccaga aagaagccat ccgcgcggaa gttgacctga ttatggaagg
     2161 catccgcaac cagcttgagg accaggaagc tgttaccaag accgaaaacg cgaagctgcg
     2221 cgacgagctc tacgaactgc agcctttcac tttcctcagt gaagcccgct atcgcgagct
     2281 caagtcacgc tgggggcagg tcttcaaggc cgatatgggc gcagaagctt tccacgacat
     2341 cctgcaccgc cttgatttga atgccctggc cgaggacctg tggcaggaag tgcgcacgac
     2401 ccgcagcaag caaaagcgga agaaagccac cagccgcttg aaagttgtcg aagctttccg
     2461 ccgttcgtcc aaccttcccg agtggatgat tctcacggtt ctgccggtta ttccgcccga
     2521 cctgcgcccg atggtccagc tcgacggcgg ccgctttgct acctctgact tgaacgacct
     2581 gtaccgccgc gtcatcaacc gcaacaaccg cctcaagcgc ttgttggaac ttggcgcgcc
     2641 ggatgttatc gtgcgcaacg agaagcgcat gctgcaggaa gccgtggatt ccctgataga
     2701 caattcccag cgcgggcgca tgctctccag gcgcggccgc cgtgaactgc gctccctgag
     2761 cgacatgctg aaaggcaaga aaggccgctt ccgccgaaac ctgttgggca aacgcgtgga
     2821 ttattccggg cgctctgtaa tcgtggtcgg tccgaatctg aagctatatc agtgcggtct
     2881 gccgaaaaca atggcgctgg aactgtacag acccttcgta atcgccggcc tggtggaaga
     2941 acaccactat gcccacaatc tgaaaggcgc gaagaagttt atcgagcgca accggccgga
     3001 agtctgggaa gtcttggaag aagtcattca gcggcgtccc gtgctgctta accgcgcccc
     3061 gaccctgcac agattgggca tccaggcgtt tgaacctatc ctgattgaag gcagcgcgat
     3121 tcaactgcat ccactggtga ccacagcgtt taatgctgat tttgacggcg accagatggc
     3181 tgtacatctg ccgctttcgg acaaagcggt ttcagaagcc cgtcagtata tgctggccag
     3241 caaaaatctg cttaaaccag ccagcggtga acctatcatt tctcctggaa aggatatggt
     3301 cctgggtgtg tattacctga ccatgcttgc caacagaccg aaacagggcg acggcagggc
     3361 ttttgccaat attgatgaag ttattctggc ctaccaactc gggcaggtgg aactgcacac
     3421 acgcattaaa ctggtggtgg agagctggta ttccgattcg gataaaccgg aacgcctttc
     3481 tgaggcggag aaacggctgc aagagacgac tgtgggtcgg gcaattttca atgacatact
     3541 gcctgacaaa atccgcttta tcaaccggac gctcgacaag ggcggtatcc gcgatttgat
     3601 tgcggaaatt cacgagcttc tgaatgacga agaaacaacc gacgtcgcgg acaaaatcaa
     3661 ggatatcggt ttccggtatg ccacccaaag cggttcgacg cttgccgtcg cggatatcga
     3721 ggtcccgcct gaaaaagccg acatcatcag ggaagcgctc ctgaaggtcg agacagctca
     3781 aaaagcttac cgccggggtc tgcagacgga acatgaacag aaccagaact tcatcaaaat
     3841 ctggcaggac gccaccgaag aagttggaaa agcggtcaaa gcgcatttgg accccaacgg
     3901 caaccttgcc accatggcga actccggcgc gaccaagggc ggtttcagcc cgatttccca
     3961 gttggccggc atgcgcggtt tgatggcgga tccttccggg cgcatcatcc caatgcctat
     4021 tcgctcgaac ttccgtgaag gcctgaccgc gttggaatac ttcatttcca cgcacggtgc
     4081 gcgcaaaggt ttggcggata ccgcgctgcg tacggctgac gctggttacc tgacccgccg
     4141 tctggtcgac gtcgcgcagg atatgattat caataacgaa gactgcggta cggaagaagg
     4201 catctatatc aacgcgaagg atgacgttgc cgggcagtca tttggcgaac gcatttacgg
     4261 ccgcttgtta gccgagagcg ttgttcatcc taaaacgggc gaggtgcttg ccgggcggaa
     4321 cgagctgatt gactacaaac tccagcgcgt catcttgcag gctggtgttc agaccatcaa
     4381 agtgcgttcc ccgctcacct gcgaactgct gcacggtgtg tgcgcccgct gttacggcat
     4441 ggacctggcg cgcggaaaaa tggtcaacct cggtgcggca gtcggtattg tggcagcgca
     4501 gtccattggc gaacctggaa cccagcttac cctgcgtacc ttccataccg gcggtgttgc
     4561 cgccgcaggc gatattacga ccggtctccc gcgcgttgaa gagcttttcg aggcgcgtgt
     4621 aatgccaaag ggcgaagctg ttatcagccg catcaagggt gttgtgcacc tgattcagtc
     4681 tcccaagaat cccgaccagc gcatcatccg cgtggaccac agcgaaatga cctccgacac
     4741 ctatgaaatt ccggaaggct ggcaggtcca ggttcaggat gagggtgaag tctccatcgg
     4801 cgatgtgctc gcgagcatgg aagacgcgac catcggcgcc cagcacgccg ggcgtgttcg
     4861 catcgaagac aacaacgtaa ttgtttccta cgagaaccgc gagaccgagg aatacgacat
     4921 ccctccgacc gccagactga cggtgaaaga aggccagatt gtggaaggcg gagaaccgtt
     4981 gaccgaaggt tcgctgaacc cgcataccat tctgcacatc aaaggccgcg aggcgaccga
     5041 actgtatctg atgactgaaa ttcagaaggt ctatcgcacc cagggtcaga atattaacga
     5101 caagcacttt gaagtgatta tccgcaagat gctcaacaaa gtgcaaatta cccgcccagg
     5161 cgactcacag tacctcccac aagacctggt tgaccggctg gaactgcgga aagttaatga
     5221 agatcttatc gccaaagggc tgcggccggc gcgctttatc aacgtcctgc tgggcgtgtc
     5281 gaaagcatcg ctaaacaccg actctttcct ctcggcctct tcgttccagc acaccatcaa
     5341 ggttttggct ggcgccgcga ttgcttccaa ggaagacttg ctctatggtc tgaaggagaa
     5401 cgtcatcatc ggcaagctga ttccggcggg cacaggcttt gatcccgagc gctttgcggg
     5461 gcagaacgtg gaaccccagc ctggtaaaga agacatccag gtgaagcaga tgaacatctt
     5521 catggaagac gacagttctg tactggcctt aaatgaggaa gaagattctg cggcggatga
     5581 ctattccgat gcggatgatg atccggagct tgacgacctt agggaagacg aagagtaaac
     5641 cggcagcatc aagaattacc cgacccggag cctgatacgc tccgggtttt tttatgctat
     5701 atttatgccg gatttgatac ccctggaatt ttcgatgaag cgagcttttt atggattcaa
     5761 cctctatcaa gaaaattaac atcaacaccg aaatgcagca gtcctacctg gactatgcta
     5821 tgagcgtgat tgtctcccgc gcgctgccag acgcccgcga cggactaaaa ccggtgcagc
     5881 ggcgcatcct gtacgcgatg tacgacatgg gtgttcgtcc agaccaaccg caccggaaat
     5941 cggccagaat cgttggcgag gtgctgggga aatatcaccc ccacggcgat tccgcggtat
     6001 acgaagcgat ggctcgcatg gcgcaggact tttccgagcg ctatccgctg gtggacggac
     6061 aaggtaactt cggaagcatt gacggggacc ccccggcagc tatgcgttat accgaagccc
     6121 gtctttccga agccgcggtg gaaatcctga agcagttcga catggacacg gtggagttca
     6181 ccgagaattt tgacggttcc ttacaggagc cgctcgtgct gccttcagcg cttcccaata
     6241 tgctcgtgaa cggcgcttcg ggcatcgcgg ttggcatggc cacgaatatc ccgccgcaca
     6301 acctgggcga ggttatcgac gcgctgacac tcttgctcga aaactgggac aaaattgatg
     6361 atatttccac ggaagaaatc atgcgattca tcaaaggacc ggactttccc accggcggat
     6421 taatccttca agaggaaacc accgaaacgc tggcgcaaat ttacggcagc ggaaacggtg
     6481 ttatccagat tcgcgcccga acacgcctgg aggaaatgtc ccgcggcagg atgcgcatca
     6541 tcgtaaccga gctgccgtac atggtcaata agtctgccct gattgaaaga atcgcggaac
     6601 ttgtgcgcga tggggtggtg gacggcatta ccgacctgcg ggacgaatcc gaccggcagg
     6661 gcatgcgcat cgtaatcgac ctgaacaaga atgccgaccc ggaaaaagtg ctcagcgtac
     6721 tctataaaaa gaccgcgatg cacagtacct tcgcgatcaa catcctggcg ctggtcgggg
     6781 ggagcccgca ccggctgtcc ctgaagcaga gcttgcgcgt atttgccgag caccgcctgg
     6841 aagtcatccg tcggcggagc gaatacgagc tgcgcaagaa cgaggaaagg cgccacattt
     6901 tggaggcgct gcgcgtagct attaagaaca ttgacctggt catccaaatc atccgcaaat
     6961 ccaagtcggc ggaggaagcc cgtcagaacc tgatgagcaa gctcagcctg gacgaaatcc
     7021 aggcgactgc tattttggac ctgcagctgc gcaggctggc gtcgctggaa cgtcagaaga
     7081 tcgaagatga ataccagcaa gtgagtgagg ccatcaagaa cctcaaagcg ctgttgaaat
     7141 ctccccaaaa gatgcgccag gtggaaattg acgagctgaa gcttctcaag tccaaatacg
     7201 ctgacccgcg ccggacgcag attatccgcc tgggtgaggg cgagaaggtg tcggaattgc
     7261 tgacccaaac ggatgtgctg ccgctggaaa acttctggat cgaagccagc gcggatggag
     7321 ttctctcccg cacggagggc gataaaccca accgattggg cggcgagaac gccccctgga
     7381 gcatgctacg cgcaaactct caccagacgg ttttcctggt ttcatcgcag ggtaaggctg
     7441 cagcaatcca tagtctcgcg attccggtgc agaaaaacgg cgaacagggc atcgcggcgc
     7501 ataaactttc cccgctcaat gggaatgacg ttttacaagg cattttcagc gtgccgatgg
     7561 accgcgataa gctgcccgaa acttacgtgc tgacagtctc ccgccagggc atggtcaagc
     7621 gttctgcgct gcaggaattg cccggcgcct cggcaaacag cttcgtcctg acgaagataa
     7681 acgaggggga tgaacttttc caggtgcttt tgaccaatgg aaaacaggat atcctgatga
     7741 ccactgccaa cgggatgagc atccgcttcc atgagtctga aattcgtccg atggggctga
     7801 ttgctgccgg tgtgaacggc atgaaattga aagaagggga ttacatcgta ggcgtcagcc
     7861 tggtggataa caaagacagc gttgcgcttc tgacgcgcaa aggtctggca aagcgggtga
     7921 gcgcggagga ctatccgctc cagggcagat acggacaggg cgtcatcagc tggaagctgg
     7981 caaaagacga ccagattgtg gttcagacag ccggcaggct ctcggacaaa gtcatttgcc
     8041 acttccgcaa gtcagccagt aaggtattca ccatcacgag cgcggtagaa cggacgcgcg
     8101 cagccaatgg tcaaggagta ttcacgctga aagccgggga cgagttgatt ggcgcgacgc
     8161 cgctggatga cctttcagct tactgggaga aagcctgatc aggatgcctg gcgaaagctg
     8221 ccgaccaggt ccaggtctgg tgcgattgcg cgcatcgcgc tgattacgtt gctgacgtgt
     8281 tcccagaggt caacgccaaa ttcctgcgcg cccaattcca tcatcgtccg gtccgttccg
     8341 gctgcgaagg ccttatcttt ccattttttg cggaccgaac ttgcttccag gtcaaatagc
     8401 gagcgggacg gccgaaccaa tgctacggcg gtaatcaggc ctgtgatttc atcgcaggca
     8461 aacagggctt tcttgagcgt ggtatcccgc ggcaggccgg tatggtcagc atgggagagc
     8521 accgccgcga tgatatcttc cgaaacgccg gcctcccgca gaaggggcgc gccagcgatt
     8581 ggatgttcct cagcattggg atggatttcc caatcaaaat cgtgcagcaa gcctgccata
     8641 gcccattcct cttcgttttc gcccaacttg cgcgcgtaaa aacgcatggc tgcttccacc
     8701 gagagcatat gccggatgag gctttcactt ttcacgtatt ggcggataaa ttgcaaggct
     8761 tcatcacgat tcatagcggc tccgtttatg ggaatatagg ttctggctgg cccaaaattg
     8821 gcaggggttt cgcgatatag atatcccaaa ctgccgagag cgtggcaaaa acttcgcgca
     8881 cctgccagaa aatgtagcgg gtgcggacgt agcgagactg ttccacttcc acgccctgaa
     8941 tgttaatccc caggcgatcg cacaggaata ttgcccgcgg caagtgataa ggctgggtcg
     9001 tgatgacagc cgtctggatg ccaaaaattt cacgcgcgcg gtagcaggta tcgtaggtgc
     9061 ggcggccggc ataatccagc acgatatctt cgtctggtac cccgagcgaa agggcatatt
     9121 ggcgcatgga ttcgggttcg ttgtaatatt cggtactgtt atctccgctc ataaggattt
     9181 tctgcgcctt gccagccagg tacaggtcca ccgcggcctg gacccggtcg cgcagcggca
     9241 cggagggcgt gccgtcagcg ctgagcccgg ccccgggcac aatcaccacc ggaaccggaa
     9301 tcacctccga aatcgggacg gttttgctgg cggcgtagaa aatcatgccc aggcgcagta
     9361 agcccaccaa aataaaaccc gcgcaaaaac aaaccacgag gacctgcaaa aacctccgca
     9421 tcgtgttgtt ccttaacctt ctcagggaag cagattggtc aggcgcaggt tctgaactgc
     9481 caggagttcg cgcagcgcgt cgtatgcgct gtccggctgc ggttccaggc gggcgatttc
     9541 gtaggcgttc aattcggaca actgctgtcg gccggattcg gtgcggctga gctcgagcaa
     9601 atagttggtg atttgcgcgc tcactgataa gggcagcagc gggctgactg aaaaattgag
     9661 gttggggatg actgccgggg aaagccagat gatcggcagg cggtcaatca catccggcaa
     9721 gtccgtgatg acctccgaag aagtgcgcgg atcgctggca atggcgtagg tggcggtgaa
     9781 gtcgcacacg cctttgatgt acagggcgcg aatggcggcg ctgtaggtcc tggtcagcac
     9841 cgggctttgg gtcgtcaccg cgttttgcgc caggtaaccc agcggcaccc aatacccggg
     9901 cagagatttc tcattggtca ggcagggcgt caggccggcg aactgggcca gcgcggtggc
     9961 ggctggcgcg gttgccaggt tgctgccagc gtcgtaataa cgggtcaggt tggcgtcttt
    10021 gtgaccaagg aactgcacgc cgtaagcagt cacgcccaga tgattggcca ccaattgcga
    10081 attgagcaag cctttcgcgg atgccagcag atactcgacc ggattcagcc aggcgagttg
    10141 aacctcctgc ttttgcaagg cttcctccag gctgaggtaa tccgggaatg ttttcccggc
    10201 tacggagagc cccaaaaaac cggaaagctg cgcgctcagg ctgtccaatc tgctaatctg
    10261 aacgctgtcc gcttctgcgc tgataacggc catgacgatc gggttttggg ggctgcccaa
    10321 cggcgcgctt gtgggggtaa aggtcggcat gggcgtggag gtggacagga aagcggtcgg
    10381 cgtaaccccg gcaggcagag cggagggtcg ggctgccggc tgggctgcgc aggcggaaac
    10441 cagcacgcac agcacgagca ggaagaatga atagcgcttg cgatattctg acacaccaag
    10501 attttaccct atctagcgta atccgcattc caaatgcgtg gtatgttaga atgcaaaaca
    10561 ataagcgcgg aggttctgtt atgaacatgg aagtggtaca gattcaaaag cctgaaggga
    10621 ttaactttat cctgggtcaa tcgcatttca tcaaaaccgt ggaagacatc cacgaagcgt
    10681 tggtcactgc cgtgcccggc atccgctttg ggctggcttt ttgcgaagcc tcgcatcccc
    10741 ggctggtacg gtggagtggt aacgacgaaa tcatgctgca gcttgcgcgg gaaaacgcgc
    10801 tgaaaatcgg cgcggggcac tgcttcatcc ttttcctggc ggagggattt tatccggtga
    10861 atgtgctcaa tactgtcaag caggtgcccg aggtttgccg cgttttctgc gcgactgcca
    10921 atccgaccga ggtaatcatc gcggtaacgg agcagggtcg cggaattctg ggagtggttg
    10981 acggtcagcc cccacttggc attgaagccg aagaagatat cgccgcgcgc aaagccttac
    11041 tgcggcgttt cggttacaag ctgtaatttt caaaggaaac gcatggatat tctcacggca
    11101 attttcaccc gaaggagcgt caggcgcttc acgcaggagc ctgtttctga cagcgacctg
    11161 gaaaccctgc tgcgcgcagc aatgcaggcg ccttcagcgg ataacgggca gccctggcac
    11221 tttgtcgtcc ttaacgagcg cgccttgatg gaccaaatcc cctccattca tccctggtct
    11281 gacatgatga aggaagccgc gctcggcatt cttgtgtgcg ggcgcattgt ggaaggcgaa
    11341 atgggcgggt actggcagca ggactgcgcc gcagcggcag aaaacatcct tttagctgcc
    11401 catgggattg gattgggtgc ggtttggctg gggatttgcc cggaggcgca gaggatggcc
    11461 gacttttctg ctctggtggg tttgccggcg gacgtgcatc ccctggcgct catcgcggtc
    11521 gggcatcccg ggaggattgc acaggctgag aaccgctacg acccgtccaa gttgcactac
    11581 aaccgctggt aaactggttt attcccctgc gaattcacgc agggcaggca gggctgcttg
    11641 taaagcccgc gcgcggtggc tgagctgatt tttctgttca ttgggcaatt cagccatcgt
    11701 tttgtctgag cccgccacca gaaagatggg atcgtatcca aaaccgtttt ccccccgaaa
    11761 ttcggggatg atttcgccag ggcattctcc ccacgcgcgc agcatctctc cggaagagcc
    11821 gcaaacgacc accgcgcaca caaagcgggc tttccacgga cgcgctgcct cttttagttt
    11881 ttccagaaga taaaggcagc ggtcgcggtc tgtggctgag gtcagcgggg aatagcggtg
    11941 ggaacgcaaa ccgggctctc cgcccagggc ttccacttcc aacccggaat catccgccag
    12001 cgccgggatg ccggcagcgc gcgcgtacgc agaggctttc aggtgggcat tgccagtata
    12061 gctgccgctg tcctccacaa cggaaatatc caaaccgaaa tctgccggag aaagtaatct
    12121 cagcggaata ccagccagga tagcggtaat ctcctgaatt ttgccaggat tagtggtcgc
    12181 aagcagcaag tctttcatcc gtctaagctt ctccagggca ggtcgcgcgg catgttataa
    12241 tgccgatatc agataggaat tgtactttgt caatggaaaa acaacactat tctttacgcg
    12301 caaacgtcgg atttttcttc aataaaccga ttggcttctc ccgtgatttt cacgcagaat
    12361 ttcctgaaat ctacctcgaa ccggacctga acgtcctgaa ccttgcggct gtttaccgct
    12421 tctcccgcac ccgggaaggt ctgctgctgc agaccgagct cagcggtgag atccaggctc
    12481 agtgcgtacg ctgcctgacc gaaagcagcg tgccggtgag cacaagcttt gaagagctgt
    12541 tctacttccc tgaacgcacc caggaagaaa ccgaccagtt ggtgcccacg gacggctaca
    12601 ttgacctagc cgacatcttc agggattatc tcctgctgga gataccaatc aaccatatct
    12661 gcaaaccaga ttgcaaaggc atatgcgtgg agtgcgggca gaacctgaac ctggctgact
    12721 gcagccatac tgacagccgc attctcctga cggacgaaga ataagccttt ccctggtccc
    12781 cataaatttg taatttcacg atggcctaaa atctcaaggc taaatagaaa tgtcctaaat
    12841 aggggattaa gtcatcaggg cgggttttcc ctcgaaacta agcctcttat ttcttttttt
    12901 tggattgttt cacggttgtc ttcactttga cccttccccg gcggcaagcg gctctatttt
    12961 gcctcgctca aggtgctgtg gaagggaaga atgtttgaca actccccgtc tcagcggttg
    13021 ttaattagga taaaaggtta tttttcgttc gctggaaaag ggaggaagag ggggaaaggc
    13081 tgctgggacc ttgtatggca ggggattctc attcctatgt aaaaaaagcc tggagaaatc
    13141 gaatgctgat atcctaatcc cagctcctaa tcttaatcag gacatttcta ttttggtttt
    13201 caagatatta atcctttgct cttaaacccg ggagccgaaa c
//
LOCUS       DAOQAD010000048        13210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_5183_length_13210_cov_6.856404, whole genome shotgun sequence.
ACCESSION   DAOQAD010000048 DAOQAD010000000
VERSION     DAOQAD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5183_length_13210_cov_6.856404"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..978)
                     /locus_tag="PKL60_05265"
     CDS             complement(292..978)
                     /locus_tag="PKL60_05265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNW95581.1"
                     /translation="MIPIRDEIKARRVPYVNYILIAINVVVFLFETMAGPRLEAVISE
                     FALVPTQITAGLDPGDMRSILTSMFLHASWMHLLGNMLYLWIFGDNVEDRIGHFWYLL
                     FYLAGGFVAAFAHWAVNPASSIPTVGASGAIAAVLGAYLILYPRARVYTFIPIGFFVR
                     LQLLPASAVLLLWFILQLFNGVLTLGAEDVGGTAFWAHIGGFLFGVLVGFLTKPRDVE
                     KPARQWSQIG"
     gene            complement(962..1669)
                     /locus_tag="PKL60_05270"
     CDS             complement(962..1669)
                     /locus_tag="PKL60_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNW95582.1"
                     /translation="MQRESIFKIIRFLVKALVNMRVEGYENVPESGPFIVVINHISRL
                     DTPFLMLSTTRQDTIGMVGDSYRKNAFFNWLLNKMGVIWVNRESYDFAAFREAADYLK
                     RGWIVGVAPEGTRSRNGKLKPGKPGAALLAKKTGVMMLPAAVTGSEEMADHLLHLKKM
                     EVCVRFGAPFRFDELDPNADNKVWLEEATEEMMCRIAALLPEERRGVYAGHPRVAQLL
                     SQNQAQAPEEERFDSHS"
     gene            complement(1701..2939)
                     /locus_tag="PKL60_05275"
     CDS             complement(1701..2939)
                     /locus_tag="PKL60_05275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNW95583.1"
                     /translation="MNRNAKTDWMSILQLVFSILVFFTFLGAAVLTAAAGSALTGIAG
                     LEGLNPGSQSITACAFAALALLNLPSAVTSMRRLTGARQHAPARAGSSQRKPALPGWL
                     PLLLFGGALAGMYYLPESLPRRELLVGLLAVLGAVLPAVWLLRVGSGSQWGSFANRHA
                     GLLSFSIGFTTYFILVLEMAVIFLLGAGVGLAAAANPSLMTRLAELWRVYLTSGADPE
                     ALRASLMEFARSPLVLALTFGLVALALPMLEEGFKTLGVWLLSDRKISPAEGYAAGLF
                     CGAGFALVEAFLNSAQMAGGNSAEWLAFLLLRFGGTLLHCFNGGLMGWALAKSWQDKR
                     RGRTLGVFLLALLAHALWNAAALADLFFDAQSGSGMTAYLPMILLGAVLLGCFLMFSR
                     RVHRSGSGIPADSPNGTSFY"
     gene            complement(2963..4294)
                     /locus_tag="PKL60_05280"
     CDS             complement(2963..4294)
                     /locus_tag="PKL60_05280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNW95584.1"
                     /translation="MKKLSGVILVLLIGALVLGACKVREFTATPEPTATAEATEAPAA
                     EATEAAEATAAPTEVVDTRLFDENGKMICTIYEGFFPALTAEQSQSLEVFPEISDADW
                     TRGPEDATLTIMEYSDFQCPACAAFYAELEKLMEKYPDDVRLVFRNFPLVSIHPNSQV
                     AAQAAEAAGLQGKYWEMYHDLFSKQGDWSGLAAEDFLSWLKTEAETLGLDADQFAADL
                     TSEAIVNKVASELEYGQSTIGLNSTPTILLNGRPWSYDWSASTLGMVIDVLKYEKGNL
                     ETECPPWVIDQSKSYTATIKTEKGDIVIQLFDDEAPITVNSFVYLAEKGFFDGVTFHR
                     VLHDFVAQAGDPSGTGISGSGYEFRDEIVSSLTYDEAGMVGMANAGANTNGSQFFITY
                     VPATQLTGKYTLFGKVIEGMDVLQSLAERDPQNNPDAPAGDKIISITIEVK"
     gene            complement(4324..4980)
                     /gene="tmk"
                     /locus_tag="PKL60_05285"
     CDS             complement(4324..4980)
                     /gene="tmk"
                     /locus_tag="PKL60_05285"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNW95585.1"
                     /translation="MFITLEGPEGSGKTSQMRELAAYLQGMGYEVLTTREPGGTDIGD
                     QIRQVLVRLENQGLHPRTEILLFLAARAQLVEQVIKPALSAGKVVLCDRYGDSTLAYQ
                     GYGHGLDLATLRSMLAFATDQLKPDLTLLLDLDVEAGLQRKRKEAEWNRLDAYEVAFH
                     HRVRAGYLELSAQEPSRWRVIDAGASKDAVQLAMREAVSRFITENTKEPLSAPANQGN
                     "
     gene            complement(4983..6611)
                     /locus_tag="PKL60_05290"
     CDS             complement(4983..6611)
                     /locus_tag="PKL60_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNW95586.1"
                     /translation="MTTTQKKITDDLDAMLSVLPPDIARAVQSRDNFDNLLEVILDLG
                     RLPTARFVDEEVPLSTQNVTQADIDYVTTRIGDFDADNRAGMERTLHRISAIRNRRGK
                     IVGLTCRVGRAVYGTIDIIADLVNSGQSILILGKPGVGKTTMLREAARILAETRRVVI
                     VDTSNEIGGDGDVPHPAVGAARRMQVPKPSLQHEVMIEAVENHNPEVIVIDEIGRELE
                     AQAARTIAERGVQLIGTAHGNSLENLLLNPTLSDLVGGIESVTLSDEEARRRGTQKTV
                     LERRAPPTFTVLVEIQSRQRLAVHRDVSASVDAQLRGYLAPPEERYWDEKGGIHIQSA
                     PQPAVPTVSQALKRGSIEKAPFQRGLNQDRTRVNPAPVIPPNAVYESAPSTKAPEMKN
                     IFAFGIARNRLLQRIRGMGVPARLVRTLEEADTFVTQRRYYRERLQPIVQAEEQGLPI
                     YVIKSNSDSQIEQFLADVFMVAVPNHVSSPYIEALEEADDAVQMVMNGEKYVDLKPAP
                     ADVRREQHQLAEKAELASESFGTEPRRFVRIFRD"
     gene            complement(6782..7525)
                     /locus_tag="PKL60_05295"
     CDS             complement(6782..7525)
                     /locus_tag="PKL60_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95587.1"
                     /translation="MWHAEGDAFSRLDYVACLPDASPDDLTCLLEALVRVSGSWGARY
                     LLAELPESSPCLPAFRRADFSIWSQARIYRYEGSFGQSEKAENSWRQWTDQDVRAMQA
                     LRQSAVPKLYQTIEPLSRKLTQGLAAYDLQGKLLTFADLAYGPTGIWLQPLLDPAADH
                     ASLLLSLTQAVPKRLGRPLYFSARSYQPLISEALERLPFSLERELALMVRHLTLKNKV
                     PAVGWQPGYEGEKIEGSVPVIQSRQTSVK"
     gene            complement(7708..8292)
                     /gene="pdxT"
                     /locus_tag="PKL60_05300"
     CDS             complement(7708..8292)
                     /gene="pdxT"
                     /locus_tag="PKL60_05300"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNW95588.1"
                     /translation="MKIGVLALQGDFLEHRRSLSSLGVESAEVRLPAQLDGLDGLIIP
                     GGESTTIGKLMVAYGFVDALKQFAREKAIWGTCAGAILLSQDAGRDQDLLQVMDIHIA
                     RNAFGRQVDSFETELPAPFLSPAEPPFHAVFIRAPIITHVYGRARALITLADGRVVAA
                     RQGKLLATSFHPELTPDTRFHRFFLELAQSPHAE"
     gene            complement(8310..9197)
                     /gene="pdxS"
                     /locus_tag="PKL60_05305"
     CDS             complement(8310..9197)
                     /gene="pdxS"
                     /locus_tag="PKL60_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNW95589.1"
                     /translation="MEQQVSGFAVKKGLAQMLKGGVIMDVVTPEHARIAEDAGAVAVM
                     ALERVPADIRASGGVARMSDPELIVRIMETVSIPVMAKCRIGHFVEAQILEALGVDYI
                     DESEVLTPADEAHHIYKHAFKVPFVCGCRNLGEALRRIGEGAAMIRTKGEAGTGDVVE
                     AVRHARQVLGDIRALQALPEEEVMAFAKNIGAPYELCLEVRATGRMPVVNFAAGGIAT
                     PADAALMMQLGVDGVFVGSGIFKSGDPAKRAAAIVKATTHYQDAKLLAEVSRNLGEPM
                     VGRQVSSIPEQDLIAKRGW"
     gene            complement(9260..9784)
                     /locus_tag="PKL60_05310"
     CDS             complement(9260..9784)
                     /locus_tag="PKL60_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNW95590.1"
                     /translation="MGLNGKKIAILVAEGVEDLEYSVVAMRLQEEGAEVLSAGMDLKP
                     LRGKNGLPIVPDTLIESLKADELFALVLPGGWAPDKLRRYAVVKDLIAKVHAQGKIIG
                     IICHGGLLAISAKIITGGHATGSLGIKDDLENAGAIWLDTPAFREGNLVWGRVVADIP
                     AFNKELVAALLEKA"
     gene            complement(9871..10644)
                     /locus_tag="PKL60_05315"
     CDS             complement(9871..10644)
                     /locus_tag="PKL60_05315"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNW95591.1"
                     /translation="MKKTRMGITVLLVLCAFLLAPLSRVSAQTEQPYRVSLRRDFGYG
                     SGMDIQGRMTLSLKGDESAVSRVTFLMDSQELFSTESAPYAFSFTTDAYPSGVHQLSA
                     RVETKDGKTYSTDALTYNFLSKDAASQSTRDIILPLLGLVVGIALVSGLFQYLSTRKK
                     PQSGAQSYNGFYGGAVCGKCGRPFARSLFGLNLVAGRLERCPHCGKWSLTRRASLAEL
                     EAAEASLRASAAADEATAQPRSDLQDMLDDSRYYDPNGK"
     gene            complement(10654..11448)
                     /locus_tag="PKL60_05320"
     CDS             complement(10654..11448)
                     /locus_tag="PKL60_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNW95592.1"
                     /translation="MKHIRTIINKEWAEVFKNRTVLYTTLLLPLVFAILPLIMLKSTA
                     GSIPDMGDAATTGLPEGFVQSCEGLSGGDCLQVYLVSEFLFLFMMMPVIIPINIAAYS
                     IVGEKTTRSLEPLLATPITTVELLAGKCLAAVLPAIGATYFSIGIFNLGLSLLKISPG
                     VISYVNSPIWLLALLALGPLLSLISTLFALFISSRVTDPRVAEQISAVLIVPVMGVML
                     AQMAGVVTLNLTMMLIAIAAVAALAFAMLHLGNAIFERENILTRWK"
     gene            complement(11451..12392)
                     /locus_tag="PKL60_05325"
     CDS             complement(11451..12392)
                     /locus_tag="PKL60_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95593.1"
                     /translation="MIITRGISKVFKIGKKQKQEFTAVDGLDLQVNEGEVFGFLGPNG
                     AGKTTTVRMLTCLIQPSAGQASVNGLVVGRDDTQIRRTVGLLTESPGMYERLSAEKNL
                     SIYAKLYDVPDVDAAVNKYLVMLDLWERRFDPVGSFSKGMRQKLAVARALLHEPKVLF
                     LDEPTTGLDPEAAKTVRDFLYELKNEGRTIFMTTHNLDEAERLCDRVGIFKQSLLAVD
                     TPEALRQRLYGRKVVFHLAAVNPEWVGLVAGQPGVKAAEAVENKLVVSLNEPERENPA
                     IVRALVQAGADIRFIGELRHSLEQVYLELLHSDAEGR"
     gene            complement(12861..>13210)
                     /locus_tag="PKL60_05330"
     CDS             complement(12861..>13210)
                     /locus_tag="PKL60_05330"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNW95594.1"
                     /translation="LDSGEATVTITVTPANDAPVAVGATYDLVENGSIVFNLDASDVD
                     GDELSFIVTVQPLHGSLECTGAVCTYTPMPGWYGQDSLSFKVNDGTLDSNEAVVTLRV
                     TALPRIYLPIAFK"
BASE COUNT         2720 a         4068 c         3716 g         2706 t
ORIGIN      
        1 gttgggttgc gctggcgctt cacccaacct actcacctgc cgttgggttg cgctgacgct
       61 ccacccaacc tactcacctg ctgttgggtt gcgctggcgc tccactcaac ctactcacct
      121 gccgttgggt tgcgctggcg ctccgcccaa cctattcacc tgccgttggg ttgcgctggc
      181 gcttcaccca acctactcac ctgccgttgg gttgcgctga cgctccaccc aacctactca
      241 cctgccgttg ggttgcgctg acgcttcacc caacctacct atccgccaaa cctacccgat
      301 ttgcgaccac tgccgcgccg gcttttccac gtccctgggc tttgtcagaa aacctaccag
      361 cacgccaaac aggaacccgc cgatgtgcgc ccaaaaagcc gtgccgccca catcctccgc
      421 gccaagcgtc agcacgccgt tgaaaagctg caggataaac cacagcagca gcactgccga
      481 agcgggcagc agctgcaagc ggacaaagaa accaatcggg atgaaggtgt acacgcgtgc
      541 gcgcggatac aaaatcaggt acgcgcctaa aaccgccgcg atggcgccgc tggctcccac
      601 tgtcgggatg ctggaagccg gattgaccgc ccaatgcgcg aaagccgcca caaatccgcc
      661 cgccaggtaa aagagcaggt accaaaaatg gcctatacgg tcttcgacgt tgtcaccaaa
      721 aatccacagg tagagcatgt tgccgagcaa atgcatccag ctggcgtgca ggaacatgct
      781 ggtcaggatg gaacgcatgt cgcccgggtc caggccggcc gtaatttgcg tgggcaccag
      841 cgcgaactcg gatatcacgg cttccaggcg gggaccggcc atggtctcaa aaagaaagac
      901 aaccacattg atggcaatga gaatgtaatt gacgtaaggc acgcgccggg ctttaatttc
      961 atcacgaatg ggaatcaaat cgttcctcct caggtgcttg cgcttgattt tggctcaaca
     1021 gctgcgccac acgcgggtgg ccggcataca cgccgcgccg ttcttccggc agcagggcgg
     1081 caatccggca catcatttct tccgtagctt cttccagcca gactttgtta tccgcgttgg
     1141 ggtcaagctc atcaaaacgg aaaggggcgc caaaacgcac gcatacttcc atcttcttca
     1201 ggtgcagcag atggtcggcc atttcttccg agccagtcac cgcggcgggg agcatcatca
     1261 cgccggtttt ctttgccagc agcgctgccc cgggttttcc gggcttcaat ttaccgttcc
     1321 tggaacgcgt cccttccgga gcgacgccta caatccagcc gcgcttgagg tagtccgccg
     1381 cttcgcggaa ggcggcaaaa tcgtacgatt cgcggttcac ccaaatgacg cccattttgt
     1441 tcagcagcca attgaagaag gcattcttgc ggtagctgtc gcccaccatg ccgatggtat
     1501 cctgccgggt ggtggagagc atcagaaacg gtgtgtccag gcgggatata tgattgatta
     1561 ccacgataaa agggccagac tccggcacgt tttcatagcc ttcaacgcgc atgttaacca
     1621 acgccttgac cagaaatcgg ataattttaa agatactttc gcgctgcata ctctcttgtc
     1681 acaattctaa tacgttcggg ctaatagaag gatgttccgt ttgggctgtc ggcaggtatg
     1741 cctgagccgg agcggtgcac gcgccggctg aacatcagga agcagcccag caggacggcc
     1801 ccgagcaaaa tcatcggcag gtaagcggtc attccacttc cactttgcgc gtcaaagaaa
     1861 aggtccgcaa gcgcggcggc gttccacagg gcatgcgcca gcagcgccag cagaaacacg
     1921 cccaacgtcc ttccgcggcg cttatcctgc caggattttg ccaacgccca gcccatcaat
     1981 ccgccgttga agcaatgcag cagcgtgccg ccaaagcgca acagcagaaa tgccagccat
     2041 tcggctgagt ttccgcctgc catctgcgcg ctgttcagaa atgcctccac cagggcaaag
     2101 cccgccccac agaacaaacc agcggcgtag ccctcagccg ggctgatttt gcggtcgctc
     2161 aacagccaga cgcccagggt cttaaagcct tcttccagca ttggcagcgc cagcgccacc
     2221 aggccgaatg tgagcgccag cacgagcggg ctgcgtgcga attccatcag gctggcacgc
     2281 agcgcctccg ggtccgcccc ggaagtcaga tacacgcgcc aaagttcagc cagcctggtc
     2341 atcaagctcg ggtttgcggc agccgccaaa cccacacccg ctccaaggag gaagatgact
     2401 gccatctcca acaccaggat aaagtacgta gtgaagccga tgctgaagct gagcaagccg
     2461 gcgtgccggt tggcaaagct gccccactgg ctgccgctgc cgacgcgcag cagccagacc
     2521 gccggcagaa ccgcgcctaa tacggccagc aaacctacca gcagctcgcg ccggggcaaa
     2581 ctttccggca ggtagtacat cccggccagc gctccgccaa aaagcagcag cggcagccaa
     2641 cccggcaggg cgggcttcct ttgcgaactg cctgctcgcg cgggcgcatg ctgccgggcg
     2701 ccagtcagcc ttctcatgga agtgaccgcg gaaggcaggt tcaacaaggc cagtgccgcg
     2761 aaggcgcagg ctgtgatgct ctggctgccc gggttcagcc cttccaaacc cgctattccg
     2821 gtcagcgcac tgccagcggc agccgttagg accgccgcgc ccaggaaagt aaagaagacc
     2881 aatatagaaa agactaactg gaggatactc atccagtcag tctttgcgtt cctgttcatt
     2941 tgctgagcct gaggcccaac gctcatttca cctcaatggt gatgctgatg attttatcgc
     3001 cggcaggcgc gtccgggttg ttctgtgggt cgcgctcggc caggctctgc aaaacgtcca
     3061 tgccctcaat cactttgcca aagagggtgt acttgccagt caactgcgtg gcgggcacat
     3121 aggtgatgaa gaactggctg ccgttggtgt tcgccccggc gtttgccatg ccgaccatgc
     3181 ctgcctcgtc gtaggtcagg ctgctgacaa tttcatcgcg gaactcatag cccgagccgg
     3241 aaatacctgt gccgctgggg tcgcctgcct gcgccacaaa gtcgtgcaat acgcggtgga
     3301 aggtcacgcc gtcgaagaaa cctttttccg ccagatacac gaaagaattg accgtgatcg
     3361 gagcttcgtc atcgaacagt tgaatgacga tatcgccttt ctcggtcttg atggtggcgg
     3421 tataagactt ggactggtca atcacccacg gggggcattc ggtttccagg ttgccctttt
     3481 cgtatttcag cacgtcaatc accatcccca gggtgctcgc gctccagtca taagaccagg
     3541 ggcgtccgtt cagcagaata gtaggcgtgg agttgagccc gatggtactc tgaccgtatt
     3601 ccaattcaga agcgactttg ttgacaatgg cctcgctggt caggtcagcc gcaaactggt
     3661 ccgcgtccaa acccagcgtt tccgcttcag ttttcagcca gcttaaaaag tcttccgcgg
     3721 ccagcccgga ccaatctcct tgtttggaga agagatcgtg gtacatctcc cagtatttgc
     3781 cttgcagtcc ggcagcctcg gcagcctggg cggctacctg cgagttgggg tggatgctga
     3841 ccaaagggaa gttgcggaac accaggcgga cgtcgtccgg gtatttttcc atcagctttt
     3901 ccagctcggc atagaaagca gcgcaagccg gacactgaaa atcgctgtat tccatgatgg
     3961 taagcgtggc gtcttccggc ccgcgcgtcc aatccgcgtc agaaatttcc gggaagactt
     4021 ccagcgactg ggattgttct gctgtaagcg ccgggaagaa gccctcataa atggtacaga
     4081 tcattttgcc gttctcatcg aacaagcgcg tatccactac ttcagtaggc gccgcagtag
     4141 cctcggccgc ttccgttgct tccgcggccg gcgcttcggt cgcctcagcc gtcgcggtcg
     4201 gttcaggggt tgctgtgaat tcacgtacct tacaagcgcc caaaaccagc gcgccaatca
     4261 acagtacgag gattactccg gatagttttt tcataggatt acctttcatc tgagctttcc
     4321 gcgctagttt ccctggttcg caggggcaga aagcggttct ttcgtattct cggtaatgaa
     4381 gcgcgaaaca gcctcccgca tggcaagctg aacggcatct ttagacgcgc ccgcgtcaat
     4441 gacgcgccaa cgcgaaggct cttgcgcgct aagttccaga tatccagcac gcacgcgatg
     4501 atgaaacgcg acttcatacg cgtccaggcg gttccattcc gcttctttgc gtttgcgctg
     4561 caggccggct tccacgtcca ggtccagcag gagcgtgagg tcgggtttta gctggtccgt
     4621 ggcgaaagcc aacatggaac gcagcgtggc caaatccaga ccgtgaccgt agccctgata
     4681 cgcgagcgtg gaatcgccgt agcggtcgca cagcaccact ttgcctgccg agagcgcggg
     4741 tttgatgacc tgttcgacca gttgggcgcg cgccgccagg aacaggagga tttctgtgcg
     4801 cggatgcaag ccctgatttt ccaggcgcac cagcacctgc cggatttggt cgccgatatc
     4861 ggtgccgccc ggttcgcgcg tggtgagcac ttcatagccc atgccttgca ggtaagctgc
     4921 cagttccctc atctggctgg tcttgccgct gccttccggt ccttccaggg tgataaacat
     4981 gctcaatctc tgaagatgcg cacgaagcgg cggggttccg tgccaaaaga ttctgacgcc
     5041 agttcagctt tttctgccag ctggtgctgt tcgcgccgga cgtccgccgg ggcgggcttc
     5101 aggtccacat acttctcgcc gttcatgacc atctggacag catcgtcggc ttcctccagc
     5161 gcttcgatgt atgggctgga cacgtggttc ggtacagcca ccataaatac gtccgccagg
     5221 aactgctcaa tctgactgtc agaattggat ttgattacgt aaatcggcag accctgttct
     5281 tccgcctgca caataggctg gaggcgttcg cgatagtagc gcctttgggt cacgaaggta
     5341 tccgcttcct ccagcgtgcg caccagtcgg gcaggcacgc ccataccgcg aatgcgctgc
     5401 aacaagcggt tgcgcgcgat gccaaaagca aagatatttt tcatctccgg ggctttggtg
     5461 ctgggcgcgc tttcgtacac cgcgttgggc gggataacag gcgccggatt cacgcgggtg
     5521 cggtcctggt tcaaaccgcg ttggaaaggc gctttctcga tgctgccgcg cttgagagct
     5581 tgcgaaaccg taggtacagc gggctggggc gcgctctgaa tatgaatacc acccttttca
     5641 tcccagtagc gttcttcggg cggcgccaaa taaccgcgca gctgcgcgtc cactgaagcg
     5701 gagacgtcgc ggtgcacagc caggcgctgg cgactctgga tttccaccag aacggtgaag
     5761 gtcgggggag cgcggcgttc caacacggtc ttttgcgtgc cgcgccggcg cgcctcttca
     5821 tcggaaagcg tgacagactc aatcccaccg accaggtcgg agagcgttgg gttcagcagc
     5881 aagttttcca gagagttgcc atgcgcggtg ccaatcagct gcacaccgcg ttccgcgatt
     5941 gtgcgcgcgg cttgcgcttc cagttcacgc ccgatttcgt caatcacgat gacctcgggg
     6001 ttgtggttct ccacggcttc aatcatgacc tcatgctgca gcgagggttt gggcacctgc
     6061 atacgtcggg cagctcccac cgcgggatgg ggcacgtcgc cgtcgccgcc aatttcattg
     6121 gaggtatcca ctatcaccac acgccgggtt tccgcaagga tccgcgcggc ttcgcgcagc
     6181 atggttgtct tcccaacgcc cggcttcccc aggatcagga tgctctggcc ggaattgacc
     6241 aggtccgcga tgatgtcgat cgttccgtac accgcccgac cgacgcggca ggtgagcccc
     6301 acaatctttc cacggcggtt gcggatggcg gaaatgcggt gcagggtgcg ttccattcca
     6361 gcgcggttgt ccgcgtcaaa atccccgatg cgggtcgtga cataatcaat atccgcctgg
     6421 gtgacattct gtgtggaaag gggaacttcc tcgtccacaa aacgggcagt tggcagcctc
     6481 ccgaggtcca ggatgacttc caggaggttg tcaaaattat ctctggactg tactgcgcgg
     6541 gcaatatcgg ggggcagcac agaaagcatt gcgtccagat cgtcagtaat tttcttttga
     6601 gtcgtcgtca tttcttaccc agatgcatcg aatggcacgc gaatacatcc aagcattctc
     6661 cgtgccattt tgattttaat cgattttcaa tttgccgcat tgttgaggaa agcttttgta
     6721 tatgaatatc ctgaggattg ttccgtgcga aagcgtctat tctgaagcaa ggagcgcccg
     6781 ctcatttcac gctggtttgg cgcgactgta tcaccggtac gctgccttcg atcttttcac
     6841 cctcatatcc gggctgccaa cccacagccg gaaccttatt cttcagcgtc aggtggcgca
     6901 ccatcagggc taactcgcgt tccaaagaaa aaggcagccg ttccagcgct tcagagatca
     6961 gcggctgata cgagcgtgcg ctgaagtaaa gcggccttcc cagacgcttg ggaacagctt
     7021 gcgtcagact gagaagcagc gaagcgtggt ccgctgcggg atccagcagg ggctgcagcc
     7081 agattccggt cgggccgtat gccaggtccg caaaagtaag cagcttgcct tgcaagtcgt
     7141 aagctgccag accctgggtc agcttgcggg agagcggttc tatcgtctgg taaagtttcg
     7201 gcacagcgct ttgccgcaac gcctgcattg cccggacgtc ctggtcagtc cattggcgcc
     7261 aggaattttc agccttttcg ctttgtccaa agctgccttc gtatcgatag atacgcgcct
     7321 gggaccaaat cgaaaaatcc gcgcggcgga aagccggcag gcagggagag ctttcaggca
     7381 gttccgccaa caggtagcgc gctccccagc tgccgctgac gcggaccaag gcctccaaca
     7441 ggcaagtcag gtcgtcaggg ctggcatccg ggaggcaggc aacatagtcc aggcggctaa
     7501 aagcgtcccc ttccgcgtgc cacacccgtc caaaacagcg cgggctgccg gcttgttccc
     7561 ataccgcgct gtacgaattg atcgtaatac cgcggttgga gagccagcgt atgggggaaa
     7621 gcggatgccc gcgcgtgatc tcgctgatgc tgtccgtgct ttgcgccagc tttttcgcgc
     7681 caaggagcgc gggaaaatca gaaggaccta ttctgcgtgc ggtgattgcg ccagctccag
     7741 aaaaaatcgg tgaaaacgcg tgtcgggggt cagttccgga tggaaggaag tcgccagcaa
     7801 tttaccctgg cgggccgcca ccacgcgtcc gtccgcaagc gtaatcagcg cgcgcgcccg
     7861 tccgtacaca tgcgtgatga tgggcgcgcg aataaagacc gcgtgaaaag gaggttcagc
     7921 cggcgaaagg aacggcgccg gcagctcggt ttcaaacgag tccacctgcc gcccgaaggc
     7981 gttgcgggca atgtggatgt ccatcacttg cagcaggtcc tggtcgcgcc cggcatcctg
     8041 cgagagcaga atggcgccgg cgcaggtgcc ccagatggct ttttcccgcg caaattgctt
     8101 cagcgcgtcc acgaacccat aagcgaccat cagcttgccg attgtggtag attccccgcc
     8161 ggggataatc aagccgtcca gcccgtccaa ctgggcaggc agacgcactt ccgcgctttc
     8221 cacgcccagg ctggataagc tccggcggtg ctcaagaaag tcgccttgca aggcgagtac
     8281 gccaattttc acgcgacgct ccgcgggaat taccaaccgc gctttgcgat caggtcctgt
     8341 tccggaatgc tggaaacttg ccgccctacc atcggttcac caaggttgcg gctcacttcc
     8401 gccagcagct tggcatcctg gtaatgggta gtggctttca caatcgcggc tgcgcgtttg
     8461 gcagggtcgc cggatttaaa aataccggag cccacaaaca cgccgtccac gcccagctgc
     8521 atcatcagcg cggcgtcggc cggggtggca atccctccgg cagcaaaatt cacgacgggc
     8581 atgcgcccag tcgcgcgcac ttccaggcag agctcatatg gcgcgccgat gttcttggcg
     8641 aaagccatca cctcttcctc gggcagcgcc tgcagagcgc ggatatcgcc cagaacctgg
     8701 cgggcatgcc gcaccgcttc gaccacgtca ccggtgccgg cttcgccttt ggtgcgaatc
     8761 atggcagcgc cttcgcctat ccgccgcagc gcctcgccca ggttgcggca gccgcacaca
     8821 aaaggcacct tgaacgcatg cttgtagata tggtgggctt catctgccgg ggtaagcacc
     8881 tcggactcat caatataatc cacgcccagc gcttccaaaa tttgagcttc cacaaaatga
     8941 ccaatgcggc attttgccat tactggaatg ctgaccgttt ccatgatgcg cacaattaac
     9001 tcagggtcgc tcatacgggc aaccccgccg ctggcgcgaa tatccgccgg gacgcgttcc
     9061 agcgccatca cagcgaccgc gccggcatct tccgcgatgc gcgcgtgttc cggcgtcacc
     9121 acatccatga taacgccgcc cttaagcatc tgtgccaggc ctttcttgac agcaaaacca
     9181 gaaacctgct gttccatgca acctccaaac ccaatagtat tctattggga ttttaccacg
     9241 cttggcatgt cttccgtgct taagccttct cgagcagcgc ggcgaccagt tccttattaa
     9301 acgcggggat gtccgccacc acgcgccccc acaccaggtt gccctcgcgg aaggcaggcg
     9361 tatccagcca gatcgctcca gcattctcca ggtcgtcctt gattcccaaa gaaccggtgg
     9421 catgcccgcc tgtaataatc tttgcggaga ttgccagcag cccgccgtga cagatgatgc
     9481 cgataatctt gccttgcgcg tgcaccttcg cgatcaggtc cttcacgacc gcgtaacggc
     9541 gcaatttatc cggcgcccaa ccgcccggca gcaccagcgc gaacagctcg tcagccttca
     9601 gagattcaat cagcgtgtcg ggcacaatcg ggaggccgtt cttgccgcgt aaaggcttca
     9661 aatccatccc cgcggaaagc acctcggcac cctcttcttg caggcgcatg gccaccacgg
     9721 agtactccag gtcttccacg ccttcggcta ccaaaattgc gattttcttt ccattcagtc
     9781 ccatcgaaag ctcctttttc tgtttctctg aagagagtct accaagcccg gcgcgttttt
     9841 accaggcgaa agcttcggca ggcagccggc ttatttcccg ttggggtcgt aatacctgga
     9901 gtcgtccagc atatcctgca aatcgctgcg cggctgagcc gttgcttcgt cggctgccgc
     9961 gctggcgcgt aaacttgcct ccgcggcttc cagttccgcc aggctggcgc ggcgcgtcag
    10021 gctccatttt ccgcagtgcg ggcagcgttc cagcctgcca gccaccaggt tcaacccgaa
    10081 cagcgagcgc gcgaaggggc gcccgcactt cccacagacc gctccaccgt agaagccgtt
    10141 gtaagactgc gcgcccgact gcggcttctt gcgggtagaa aggtactgaa agagcccgga
    10201 aaccaaggca atccccacaa ccagtccgag cagcggcagg ataatatcgc gtgtactctg
    10261 gctggccgca tcttttgaaa ggaaattgta ggtaagcgcg tcagtactgt atgttttccc
    10321 atctttcgtt tccacgcgcg cagagagctg atgtacgccg gagggatagg cgtcggtggt
    10381 aaaggagaag gcatacggcg cgctttccgt gctgaacagc tcctggctgt ccatcaaaaa
    10441 ggttacgcgg ctgacggcgc tctcatcacc tttcaggctg agcgtcatcc gcccctgaat
    10501 gtccatgccg ctgccgtagc caaaatcccg ccgcaagctg acgcggtatg gctgttccgt
    10561 ttgcgcgctg acccggctca agggtgccag caggaaagca cacaagacca atagaaccgt
    10621 gatacccatg cgtgtttttt tcatagacag tcctcacttc cagcgggtca ggatattctc
    10681 ccgctcaaaa atcgcattgc caaggtgcag catcgcgaag gcaagcgccg ccaccgccgc
    10741 gatggcaatc agcatcatcg tcaggttcaa agtcaccacc cccgccatct gcgccagcat
    10801 aactcccatc acaggcacaa tcaacaccgc ggaaatctgt tccgctacgc gcgggtcggt
    10861 gacccgggag gagatgaaca gggcaaacaa ggtggaaata agcgacaaca gcggtcccag
    10921 cgccaagagc gccagcagcc agatagggct gttgacatag ctgatgacac ccgggctgat
    10981 tttcagcagg cttaaaccca ggttgaaaat tccgatggag aagtatgtcg ccccgatagc
    11041 tggcagtacc gccgccaggc acttgccggc cagcagttcc acagtggtaa tcggcgtcgc
    11101 cagcagcggc tccaggctgc gcgtggtctt ttcgcccaca atactgtacg cggcgatgtt
    11161 gatggggata atcaccggca tcatcatgaa caggaacagg aattcgctca ccagatacac
    11221 ctgcagacag tcgccgccgc tgagaccctc acagctttgc acaaagcctt ccgggagacc
    11281 ggtcgttgcc gcgtccccca tatccggaat gctgcccgcg gtggatttga gcatgatgag
    11341 cggcaggatg gcaaacacca gcggcaggag cagagtggta tagagcacag tgcggttttt
    11401 aaagacttct gcccattctt tgttgattat tgtgcggatg tgtttcatgg ttatctccct
    11461 tccgcgtcag aatgaagcag ttccaggtag acctgttcca gggagtgccg cagctcgcca
    11521 ataaagcgga tatcagcgcc ggcttgcacc agggctcgca caatagcggg gttctcgcgc
    11581 tcaggttcgt tcaggctgac caccagtttg ttttccaccg cctcggcggc cttcaccccc
    11641 ggctgccccg ccaccaaacc aacccattcg gggttcacgg cagccagatg gaagacgact
    11701 ttccgcccgt acagccgctg gcgcagcgct tcgggagtat ccaccgccag caggctctgc
    11761 ttgaagatcc cgacccggtc gcacaggcgt tcggcttcat ccaggttgtg ggtggtcata
    11821 aagatggtgc ggccttcgtt tttcagctcg tagaggaagt cgcgcacggt tttggcggct
    11881 tcggggtcca ggccggtggt aggttcgtcc agaaagagga ccttgggttc gtgcagcagc
    11941 gcgcgcgcga cggccagttt ctggcgcatg cccttggaga agctgccgac ggggtcgaag
    12001 cgccgctccc acaagtccag catcaccagg tacttgttca cggcagcgtc cacatccggc
    12061 acgtcgtaga gtttggcgta aatgctcagg tttttttcgg ctgagagccg ttcgtacatg
    12121 cccggtgact cagtcagcag gcccaccgtg cgccggattt gggtatcgtc gcgccccaca
    12181 accaggccgt tcacgctggc ctgccccgcg ctgggttgta tcaggcaggt gagcatgcgc
    12241 accgtggtgg tttttccggc gccgttgggg cccaaaaagc cgaacacctc gccttcgttt
    12301 acctgcaggt ccagcccgtc tactgcggta aattcctgct tttgcttttt gccaatcttg
    12361 aagactttac taatccctcg ggtaattatc atgggtttcc tctgttttga atgaagaacg
    12421 cactgattat agtttatcct cgtgtacaga accgtagatt ttcacaggtg cgcgataact
    12481 gtgtagggac caatctggcc agtgcagcaa gactgatgag ggtcttgttc gtgtgtgctc
    12541 cgcttgcctg cggcggtcaa cggtaggggc gaagtttcct ggaatgggca ccaatctccc
    12601 tccagggcgc tcaatacaga tgcttcgccc gtacgcctga tactcaccat gcatggcccg
    12661 atgccgccga agtgtcggct tgaaaaacgc acgccgccca cgacaaatga tgaaaagacc
    12721 cccagccccg ctgcgggcac cttcccccga aggagggtcg aaagcgccgg gggcaaagct
    12781 tccccgcttc gtgcaccatg acagaccaca aaaaagaggc tgaccttacg gtcagcctct
    12841 catggataga acgaaacagc ttacttgaat gcgatgggca ggtagatgcg cggcagcgcg
    12901 gtcacccgca gggttaccac tgcttcgttg gaatccagcg tcccgtcgtt gactttaaag
    12961 ctcaggctgt cctggccata ccagccgggc atcggcgtat aggtgcacac cgcgcctgtg
    13021 cattccaggc tgccgtgcaa gggctggacc gtcacaataa agctcaactc gtctccgtcc
    13081 acgtcgctgg catccaggtt gaacacgatc gagccgttct caaccagatc ataagtcgcg
    13141 ccaacagcca ccggcgcgtc attggccggc gtaaccgtaa tcgtaacggt tgcttcaccg
    13201 gagtctaatt
//
LOCUS       DAOQAD010000049        12958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_5327_length_12958_cov_5.751453, whole genome shotgun sequence.
ACCESSION   DAOQAD010000049 DAOQAD010000000
VERSION     DAOQAD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12958
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5327_length_12958_cov_5.751453"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="PKL60_05335"
     CDS             complement(<1..1529)
                     /locus_tag="PKL60_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNW95595.1"
                     /translation="MATSAKTKSKTKKAPAKTPVKRSTRATTARKASSTPRKKTAPKT
                     VSRQAPARATSRKAQPAPRSITISPQRKVDITGIGLIVVGVLTLLSLVIGNAGVVSKW
                     WSDLLYKAAGWGAYAAPVILILAGAWFLLRNLEQFPDLSGERMIGIILAFFNALAWFQ
                     LISQRSGAPGASGGGAAGRFILDILSRGFGRPGAVILLIAWLLICLVLLVDFSMSDLT
                     LWLAERLKDKPARFARFIKSKAAALQAKRAGRSEQSFDEEPEQAETAPEPASPKRTAA
                     PSPQTTPGAAGGGHAWALPDPEEVLLPSPAQPPDQENDQDRARVIEETLRSFNTPSRV
                     VEIRRGPAVTMFGVEPDFVESRNGKKRVRVSNIVSLADDLALALKASRIRVQAPVPGR
                     GYIGIEVPNSRTSIVSMQELVESPGFRTNPNPLKFPLGKNVAGQPFTTDLADMPHLLI
                     AGTTGSGKSVCVNAMLASYLLTLTPDQIRFVLVDPKRVELTGYNGIPHLLAPVIVDAE
                     KV"
     gene            2058..3329
                     /gene="rho"
                     /locus_tag="PKL60_05340"
     CDS             2058..3329
                     /gene="rho"
                     /locus_tag="PKL60_05340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNW95596.1"
                     /translation="MDIFELDKKTLKELRHIAEDLNFPKPIRLKREGLIVKIAQVLAE
                     QEGLEMRGGILEIMNEGIGFLRSNYRIGPEDVYVSQAQLRRYDLRSGDLVIGHVRPPR
                     ESERHYGLLKVESINGLSPDEARKRVRFEALTPIFPNYRLNLEHDPKNLSTRMINLIA
                     PIGGGQRAMIVSPPKAGKTTILKDIANAIAINHPEVRQIMSLIGERPEEVTDMDRSVE
                     AEVIASTFDEPVNAHVRMAEISLDRAKRLVENGLDVVILMDSLTRLARAYNMVVNPSG
                     RTLSGGMDPSALYPPKRFFGAARNLEDGGSLTIIATAMVDTGSRLDDVVYEEFKGTGN
                     MELHLSRRLQERRIFPAIDIERSSTRREELLMDRETLQRSWLMRRMYLQMVANPPQGA
                     GMDTAVATEAILQRMEKSKTNAEFLEGLTDN"
     gene            3357..4451
                     /locus_tag="PKL60_05345"
     CDS             3357..4451
                     /locus_tag="PKL60_05345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNW95597.1"
                     /translation="MSEQNSSQKGGGLRQKSILSWIVTLMICAGLVSVLAWQPKIARS
                     MAESNLPQVSAEPESAESVEGAALQSTPQFSLPPTDEALIREPNPHTEIDTSLRTSAV
                     EYTVTEGDSVFGIAQKFDISPETLLWSNYDILSDNPHELSIGQVLKIPPTDGIWYKWT
                     SKDTLEKVAARFSVTVDDIKLYVGNNLDLSNPVIEAGQYVMVPGGSREYMQWVIPEIP
                     RGAAGVATSPFFQCDTSGMYYGGSGYFIWPSAVHTISGNNYWSGHLAIDIGAGMGSPI
                     YAADSGVVVYAGWMNGGYGNVVIIDHNNGYVTVYAHLSAIYVNCGSAASQGMQIGSAG
                     STGNSTGAHLHFEIRYMGGFINPLTVLPFG"
     gene            4466..4539
                     /locus_tag="PKL60_05350"
     tRNA            4466..4539
                     /locus_tag="PKL60_05350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4500..4502,aa:Arg,seq:cct)
     gene            complement(4646..5047)
                     /locus_tag="PKL60_05355"
     CDS             complement(4646..5047)
                     /locus_tag="PKL60_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95598.1"
                     /translation="MDNKITIIEGPTPDFNMIEDPREEGSASAWTVGVLEGPLLYRVA
                     TTSVRSFDSQALLERCTRAWKQNQTMFLEYRDEIGLRKEAQIIAAQALSVEEGDVLQL
                     WVRRDLEESEQSDDSDELDIGDDDLPDEPPF"
     gene            complement(5180..6211)
                     /locus_tag="PKL60_05360"
     CDS             complement(5180..6211)
                     /locus_tag="PKL60_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95599.1"
                     /translation="MKKHRLLIQAAALVLVLSLFLGVGSVQASTTPTFSISAVEKDVS
                     VTIVTKDFPANKTFKVLMGEIGTRAEGGIEVATFNSGEGGVFTAKFNIPAALKGRAQI
                     AIRTEGTDNPYYAYNWFWNDASGTWPEPYEPDEEIPDCGCGNIPTFSIVSVVKGEDVT
                     IHTYNFPRNVEFTVLMGKMWTRGINGIEVAKLNSGEGGSFEATFDIPEELADDERVSI
                     RLEGTGGYFAYNWFWNNTGSEAPVPPVEGYSGYPYFFIESVVKDSKVTIKAYNLPADY
                     EFKVLMGKMWTRGIGGIQVATLETGEGGTLTATYAIPSDLVGLERISIRLQGDYYYAY
                     NWFWNNTAY"
     gene            complement(6443..6859)
                     /locus_tag="PKL60_05365"
     CDS             complement(6443..6859)
                     /locus_tag="PKL60_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNW95600.1"
                     /translation="MTFYINPYGRRLRMQALANAMNEMERDFEPEVAFPMDVKATQEG
                     YELKAYLPGVSAENVDIQIVNEVVTISGEVKLERDKRTDYLLAELPVGRFHRVISLPT
                     PLDSSKVEASLENGILTLNIPKAEEAKPKSIKITQK"
     gene            6979..7686
                     /locus_tag="PKL60_05370"
     CDS             6979..7686
                     /locus_tag="PKL60_05370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNW95601.1"
                     /translation="MNQRGKIILGAALAGLVALAGFSLYRAYLGKRSAAVIAFIRAPE
                     KHADWKMSALARCGDAPFSFPSEGFVGYLWGDSFKPFQKHQGIDIFAGTAVGLTPVYA
                     PYDGYLTREEGWKSSLIIRVPDDPLHPGTQIWLYMTHLADPQGKGLISADFPPGTREA
                     PVRRGDLLGYQGNYSGNPDSPTGVHLHFSIVRDNGSGKYLNELEFTNTLDPSPYFNLP
                     LNGSSNKDVIPLCSDAE"
     gene            7811..8644
                     /locus_tag="PKL60_05375"
     CDS             7811..8644
                     /locus_tag="PKL60_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95602.1"
                     /translation="MQEIAPNVFVEHNSLGLVTGVIRGKAGSVLIDSPSRQDEMRSWR
                     SGTARLITGEPKFLVHLDTNYDRLLSIKGTDCVIVTHINSLAQEKAKPLASKLTEESQ
                     QSGEGHDAFVSPFRLIPPEIVFTQALSIYLDDTEILLEHHAGSNFAGIWAELPARKVI
                     FVGDTVLPDQPPFLAYCDLERWLEDLSSLSGRKYRNYQIVSARSGVLDQSQVEQASGH
                     IAAIHALLSPLIERKASLDEVLALIPIIMQRYEPNANTSDMYYNRLRWGLSMYYELNH
                     A"
     gene            8662..9723
                     /locus_tag="PKL60_05380"
     CDS             8662..9723
                     /locus_tag="PKL60_05380"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNW95603.1"
                     /translation="MNQDFGYAPLVELTRGAIVECVHFGAFAVVDVFGKLLCSAGDPD
                     LITFPRSSMKPFQVLPFVESGGPEYFDLSDEELAIMCASHSGTDSHVDVLRSIHQKAG
                     LQEADLQCGVHSPSDRATSEAMRLRGELPTPYRHNCSGKHSGMLAAARMRGLPKENYL
                     ELDHPVQQRIRAVMAEMSGVNPDALIPGIDGCSAPVYALPLRAFAWAAARLCDPHELP
                     AQRQSACRKITNAMRTYPVMVAGPGKLDTVLMQVLGDKLISKGGAEGYQMIGLLPDAE
                     KGRPGIGIAMKFSDGDPDRRATYSLVTAILRVFGFAERLDSEAFRPFADEVVRNWRGL
                     TAGEKRISALAKAALAGCC"
     gene            9734..10423
                     /gene="nth"
                     /locus_tag="PKL60_05385"
     CDS             9734..10423
                     /gene="nth"
                     /locus_tag="PKL60_05385"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNW95604.1"
                     /translation="MKAAVEVSAEHRERLRLVNERLNQFFGFPIWRNPLPAVDELVST
                     FLSQNTNDLNRDKAFEAIRQRYTSWDEVCEADPDEFMQVIRAAGLANQKGPNIQAALQ
                     RIRSERGVIDLEWLRGLPLEEAREWLLSFKGVGRKTAAIVLLFSLGMPAFPVDTHIYR
                     VSGRIGLRPQAMDVDSTHQYLEAAADPGQYYALHLNLIRLGREICQARKPACARCPLV
                     ELCQYPDKVLS"
     gene            complement(10425..11747)
                     /gene="miaB"
                     /locus_tag="PKL60_05390"
     CDS             complement(10425..11747)
                     /gene="miaB"
                     /locus_tag="PKL60_05390"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNW95605.1"
                     /translation="MRYYIWTEGCQMNTADSRRLSRSLENLGFVTAANVEDADVIVLN
                     TCVVRQSAEDKALGRLSSLKSLKQQNPQLVIGLMGCLVGVHGNLALQKRFPWVDVFSA
                     PSDPQPLLDFLTNRQQADRAQSWRMNIDRMLDEEFGVAVPGEQRAVSENLPVVLGCSH
                     ACAYCVIPLKRGRERSRDALRIVRDARTFAGQGVSEIVLLGQIVDRYGLDLPGSPRLP
                     QLLSSLSEIPQIKRLRFLTSHPNWMSDELIASVAELPKVMPHIEVPVQAGDDGVLLAM
                     RRGYTNQQYRELVAKLRERIPGVSIATDIIVGFPGETETAFQKTYELLADLRLDVAHL
                     ARYSPRPGTYSARELADDVPAEEKMRRFRLLENLQESILKELNQGLLGEVVPVLFEYR
                     VKGRWYGRTPTNRIVFVDSAEDLTGQERQVKITWTGPWSLVGSLEPSL"
     gene            11842..12171
                     /locus_tag="PKL60_05395"
     CDS             11842..12171
                     /locus_tag="PKL60_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNW95606.1"
                     /translation="MEFTKTDYKRCIVVKAVGRYDSATAGELEKVLTELIPTDKDIVL
                     DMSDISFVSSAGWWSIIRVQKELKKKRRDLVLVALNDNVRDSMDLIGILQYFTVYPNM
                     VEALGNL"
     gene            complement(12234..>12958)
                     /locus_tag="PKL60_05400"
     CDS             complement(12234..>12958)
                     /locus_tag="PKL60_05400"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HNW95607.1"
                     /translation="CPGSYTLNFQGTQAVSLLPIEGSASTFFWSNLGDESNMSLERSF
                     DLTTVSGRAELSFRTWYDLEKDYDYVFLSASTDGKNWQILNTRTCTTSNPSGNSFGCG
                     WNGSSQGWISESVDLSRFVGSLVSIRFDYVTDAAVNGKGIALDDFRLDAIDYTSDLEE
                     DGGGWQSAGFVRLGNTLPQSYALRLITLGDEPGVQTITLDEKNRAELSFTIGSGVEKV
                     VLVVSGTTPLTREKAVYELQIR"
BASE COUNT         2775 a         3574 c         3787 g         2822 t
ORIGIN      
        1 actttctccg cgtcaacgat taccggcgcc agcaagtgcg ggatgccgtt atagccggtc
       61 aattccacgc gcttggggtc caccaagaca aagcggattt ggtcaggagt gagcgtgagc
      121 agatatgagg ccaacatggc gtttacgcac accgactttc cggagccggt ggtgcctgcg
      181 atgagcaagt ggggcatgtc cgccaggtcc gtggtaaagg gttggccggc cacgtttttc
      241 cccagtggga atttgagagg attggggttg gtacgaaaac cggggctctc caccagttct
      301 tgcatggata caattgacgt gcggctgttt gggacttcga tcccgatata acccctgcca
      361 gggacaggcg cctgcacgcg gatgcgcgac gctttcaacg ccagcgcaag gtcgtctgcc
      421 aggctgacga tattgctgac ccgcacccgt tttttaccat tgcgcgactc gacaaaatcc
      481 ggttcgaccc cgaacattgt caccgccggc ccgcggcgga tttccaccac acgcgaaggt
      541 gtattgaaag agcgtagggt ctcttcaatc acgcgcgcgc ggtcctggtc attttcctgg
      601 tccggcggct gagccgggct gggcagcagc acttcttcgg ggtcgggcag cgcccaggca
      661 tgcccgccgc ctgcggcgcc tggggtggtc tggggtgagg gcgcagcagt ccgctttggg
      721 gaggcaggct ctggcgccgt ttctgcctgt tcgggttctt cgtcgaagct ttgctcagag
      781 cgtccagcgc gtttggcctg caatgccgcg gctttggatt taataaagcg ggcaaagcgc
      841 gccggtttat ctttcagacg ctccgccaac caaagggtca ggtcggacat ggaaaaatcg
      901 accaacagca ccagacagat cagcagccag gcgataagca gaattaccgc gccagggcgc
      961 ccaaacccgc gcgagaggat atccaggata aagcggcccg ccgcaccccc gcctgaagcg
     1021 ccaggcgcgc ccgacctttg gctgatcagc tggaaccagg ccagcgcgtt gaagaatgcc
     1081 agaattatgc caatcatgcg ttcgccggaa aggtccggga attgctccag gttgcgcagc
     1141 aggaaccagg cgcctgccaa aatcaggatg acgggcgccg cgtaggctcc ccagccggcg
     1201 gctttgtaga gcaggtccga ccaccatttg gaaacaacgc cagcattgcc aatcaccagg
     1261 ctgagaagcg tcaacacgcc gacgacaatc aatccgatgc cggtaatatc tactttccgt
     1321 tgcggggaga ttgtgatgga cctgggcgcg ggctgcgctt tgcggctggt agcccgggca
     1381 ggcgcttgcc ggctgaccgt tttcggggca gtctttttcc ggggagtgga ggaagccttg
     1441 cgcgccgtgg tggcgcgggt tgagcgtttc accggggtct ttgcgggtgc ttttttggtt
     1501 tttgatttgg ttttggcgct tgtcgccatg gttttcctcc gacggcaaat caggtaaatg
     1561 cgattatagc attaatgttc tcaagaggct tgcgcctttc atggtgccga tcgcgtcggg
     1621 tcgaaaggcg cagccagagc tgcgagaaag catgtccgta gggaggcttg cgcctccaac
     1681 gcttccgatc gattcgggtt gaaagacgct acaaggccca tgaaaaactg tagctgccga
     1741 tcatatcctt ctgaggaacg cagcccgacc tgcgacttgc gcgccgggta tcccagcggg
     1801 agcgggcgca gtgtacaagc gcttgacaaa agttgcgcgt ttttggaaag ctgttataat
     1861 caataaatac tcatcacgaa tatcctgaca agaaagttta ttggctcttc gttaacagag
     1921 cgaaatcccg tcttattact attgaaatga aatcatccca aaaattgata acccaattga
     1981 ccataaattt gctgtcggaa tagacgcgac ggaagtgtca agggcattat gttgtaaccc
     2041 acacattgaa ggtgaagatg gatatatttg aactcgataa aaaaaccctt aaggaactgc
     2101 ggcacatcgc ggaagatttg aactttccca agccaattcg cttgaaacgc gaagggctga
     2161 tcgtgaaaat cgcccaggta ctggcggaac aggaaggcct ggaaatgcgc ggcggcatcc
     2221 tggaaatcat gaatgaaggc atcggtttcc tgcgttccaa ttatcggatt ggtcctgagg
     2281 atgtttatgt gtcccaggcg cagctgcgcc gctacgatct gcgctcaggc gacctggtca
     2341 tcgggcatgt gcgccccccg cgtgagtcgg aacggcacta cggactcctg aaggttgagt
     2401 ccatcaacgg actctcgccg gatgaggcgc gcaaacgcgt gcgctttgaa gcgctgacgc
     2461 caatcttccc gaactaccgc ctcaatctgg agcatgaccc caagaatctt tccacgcgca
     2521 tgattaacct gatcgcgccg attggcggcg ggcagcgcgc catgattgtc tctccgccta
     2581 aagccggcaa gacgaccatc ctgaaggata ttgccaacgc aatcgcgatc aaccatcccg
     2641 aagttcgcca gattatgtct ctcatcggtg aacgccctga agaagtaacg gatatggacc
     2701 gctcggtgga agccgaagtg attgccagta cctttgacga accggtgaac gcgcatgtgc
     2761 gcatggcgga aatctctttg gaccgcgcca agcgcctggt tgaaaacggt ctggacgtgg
     2821 tcattctgat ggattcgctg acgcgcctgg cgcgcgcgta taatatggtg gtcaatccct
     2881 ccgggcgcac gctttccggc ggtatggacc cctccgcgct gtatccgccc aagcgtttct
     2941 ttggagccgc gcgcaatctg gaagacggcg gcagcctgac cattatcgcc acggcgatgg
     3001 tggataccgg ttcgcgtctg gatgatgtgg tttacgagga atttaagggc accggcaaca
     3061 tggaactgca cctctcccgc cgcttgcagg aacgccgcat tttccccgcg attgatattg
     3121 aaaggtcttc gacccggcgc gaggaactat tgatggaccg cgagactctt cagaggtcct
     3181 ggttgatgcg ccgtatgtac ctgcagatgg ttgccaaccc gccgcaaggc gcaggcatgg
     3241 atactgcggt agcgaccgag gcaatattgc agcgcatgga aaagtccaag accaacgcgg
     3301 aatttcttga aggtctgacc gataattaag cggttggaca gcacgaaaga ggaaggatga
     3361 gcgaacaaaa ctcaagtcaa aaagggggcg gcttacgcca gaagagcata ctctcctgga
     3421 tagtaacgct gatgatttgc gcagggttgg tttctgtgct ggcctggcag ccaaaaatcg
     3481 ccaggtccat ggcggaaagc aacctgccgc aggtcagcgc tgaaccagag agcgctgaaa
     3541 gcgtggaggg agctgcgctg caaagcacac cgcagttttc gcttccaccg actgacgagg
     3601 cgctgatccg ggagccaaac ccgcataccg agattgacac gagtctgcgc accagcgcgg
     3661 tggaatacac agtcaccgaa ggcgactcgg tttttggcat cgcgcagaaa tttgatatct
     3721 cccccgaaac gctgctttgg tctaattacg acatcctcag cgacaatccg catgaacttt
     3781 ccatcgggca ggtgctcaaa atccctccga ccgacgggat ctggtacaag tggacctcca
     3841 aagatacgct ggaaaaggtg gcagccaggt tttctgtaac agtggatgac atcaagctgt
     3901 atgtgggtaa caacctggac ctgagcaatc cggtgattga agccgggcag tatgtgatgg
     3961 tcccgggcgg ctcgcgtgaa tatatgcagt gggtcatccc tgaaattccg cgcggcgcag
     4021 ccggcgtggc aacttcgcct ttcttccagt gcgacacgag cgggatgtat tacggcggca
     4081 gcggctactt catctggcct tccgcggtcc atacaatttc cgggaacaac tattggtccg
     4141 ggcatctggc catcgatatc ggcgcgggga tgggctcgcc catttacgcg gcagacagcg
     4201 gcgtggtggt ctacgcgggt tggatgaacg gcggttatgg taatgtggtg attattgacc
     4261 acaacaacgg ctatgtgacc gtgtatgccc acctgagcgc gatttacgtc aactgcggca
     4321 gcgcggcttc gcagggcatg cagattggct cagccggcag caccggtaat tccaccggcg
     4381 cgcatctgca ctttgaaatc cgctacatgg gcggttttat caatccgctg acggtgctgc
     4441 cgttcggatg atataatcat cgcgcgctcc agtagctcaa atggacagag caaggcactc
     4501 ctaacgccta ggttgggggt tcaattccct cctggggcac agctataaag gaacttcggg
     4561 cagctttcgg gctgcccgaa ggctatatca aggggtatcc agcaaaaaca caaaaaagcc
     4621 tctcccaaag gagaggccga tggtcttaaa aaggaggctc gtcggggagg tcatcgtccc
     4681 cgatgtcaag ctcgtcggag tcatccgact gctcgctttc ttccagatcc ctgcggaccc
     4741 acagctgcag cacgtcgcct tcttccacgc taagggcttg cgctgcgata atctgggctt
     4801 cttttctcag cccgatttca tcccggtatt ccaggaacat cgtttggttc tgcttccagg
     4861 cgcgtgtgca gcgctccaag agcgcctggc tgtcgaacga gcgcacgctg gtggtcgcga
     4921 cccggtaaag cagcggtcct tccaacacgc cgaccgtcca ggcggaagcg ctgccttctt
     4981 cccgggggtc ctcgatcata ttgaaatcgg gcgtaggacc ttcgatgatt gtaattttgt
     5041 tgtccatgat tctccagccg ctatcatacc atagacccgc tgaaagcggg ggcgattctt
     5101 gacatggtac aaagtgagga gacagagaga aacgggctgg tcccttcaga gatcagcccg
     5161 ttcgcatttc cgctgctgct tagtaggccg tgttgttcca gaaccagttg taagcgtagt
     5221 agtaatcacc ctgaaggcga atcgagatgc gctcgagccc gactaaatcg gaagggatag
     5281 cgtaggtggc ggtcaacgtg ccgccttcgc cggtttccag cgtcgctacc tgaatgcctc
     5341 cgattccgcg ggtccacatt ttgcccatta acaccttgaa ctcataatcc gccggcaggt
     5401 tgtaggcttt gatggtgact ttgctgtcct tcaccaccga ttcgatgaag aagtaagggt
     5461 agccggaata gccttccacg gggggaacag gcgcctcgct gccagtatta ttccagaacc
     5521 agttgtaggc aaagtagccg ccggtgcctt ccagccggat ggacacgcgt tcgtcatccg
     5581 ctagttcttc agggatgtca aaggtggctt caaaactgcc gccttcgccg gaattcagtt
     5641 tggcgacttc gatgccattg atgccgcgcg tccacatctt gcccatcaat accgtgaatt
     5701 caacgttcct ggggaagttg taggtgtgga tggtgacgtc ctcacctttc acaacgctca
     5761 cgatcgagaa ggtcgggata ttcccacagc cgcagtcggg gatttcttca tccggctcat
     5821 atggttccgg ccaggttccg ctggcgtcgt tccagaacca gttgtaagcg taatagggat
     5881 tatccgtccc ctcagtgcga atcgcgattt gcgcccgtcc cttaagggcg gccgggatgt
     5941 tgaactttgc cgtaaacacg ccgccttcgc ctgagttgaa agtggctact tcaatgccgc
     6001 cttccgcgcg ggtgccaatt tcgcccatca gaactttaaa ggtcttgttg gccggaaaat
     6061 ctttggtcac gatggtcacg ctgacgtctt tttccacagc agagatggaa aaagtgggag
     6121 tcgtagaggc ctgcacgctg ccgaccccca ggaaaaggga gaggacgaga acgagtgctg
     6181 ctgcctggat aagaagacga tgctttttca ttttttctcc ttggactatg ctcgggggtg
     6241 cttgtgttcc gacccgaaat tagtttatca ctcggactca ggacgtttaa attgctggaa
     6301 aagttccaca tcccgcttaa tgcttaacgc attatagcat tttcgtaggc tgaatcaaat
     6361 atttgataca gattcggacg caaataaacg aaagagcgct tgcatattca tggcagcgct
     6421 ctttctggta tcgcaatcag gattactttt gtgtaatctt gatggatttg ggtttggctt
     6481 cttccgcttt ggggatgttc agggtcagga tgccgttttc caggctggct tcaaccttgc
     6541 tgctgtccaa gggcgtgggg agcgaaatca cgcggtggaa acgtccgacc ggcagttccg
     6601 ccagcagata atcagtgcgt ttgtcgcgtt ccagtttcac ttcgccgctg atcgtaacga
     6661 cttcgttcac aatctgaatg tccacattct cagcgctgac tcctggcaga taggctttca
     6721 gttcgtagcc ttcctgcgtg gctttgacgt ccatcgggaa agcgacctcg ggttcaaaat
     6781 cgcgctccat ttcgttcatg gcattggcaa gtgcctgcat ccgtaatctg cgtccgtagg
     6841 ggttgatata aaaggtcatt ttggtctcct tttcacttca atgttttacc tgtctgtact
     6901 ctacctgcgg gatatcagat tcttgcatac aatatgtaga taatttatta gaaacaagac
     6961 gcaaaggacg caaggcttat gaaccaacgt ggaaaaatca tcctcggagc ggcgcttgcc
     7021 ggtctggtcg cgctggcggg tttctcgctt tatcgcgcgt acctgggcaa acgcagcgcg
     7081 gctgtcattg cctttatccg cgcgcccgaa aagcacgcgg attggaaaat gagcgcgctt
     7141 gcccgctgcg gggatgcgcc cttcagcttc ccgagcgaag ggtttgtcgg ttacctgtgg
     7201 ggagacagct tcaaaccctt ccaaaaacac caggggatag acatttttgc ggggacggcc
     7261 gtcgggttga ctcccgtgta cgcgccttat gacgggtacc tgacgcgcga agagggctgg
     7321 aaaagcagcc tgattatccg cgtcccggat gacccgctcc atcctggcac gcaaatctgg
     7381 ctctatatga cgcatcttgc ggacccacag gggaagggct tgattagcgc tgactttccg
     7441 ccgggcacgc gggaagcgcc agtgcgcagg ggcgatttgc ttggttacca gggcaactac
     7501 tccggtaatc cagacagccc tacaggcgtg catttgcatt tttccatcgt gcgggataac
     7561 ggcagcggca aataccttaa tgaactcgag ttcacgaaca cgctggaccc ctctccttac
     7621 tttaacctgc ccttgaacgg aagcagcaac aaagatgtca tcccgctgtg ttccgacgct
     7681 gagtagtagc aaccacggag cgcaaaagcc cggcctgccg gagagccaga cgaagcttct
     7741 cttccggttt ggcgataagg ggattcttcg ctataatacc ggcagcaaaa agtgcaggag
     7801 gtctgtcagt atgcaggaaa tcgcccctaa tgtgtttgtc gaacacaact ccctcggttt
     7861 ggtgacaggg gtcatccggg gaaaagcggg ctccgttctc attgattccc ccagccggca
     7921 ggacgaaatg cgttcctggc gcagcgggac ggcccgcctc atcaccggag aaccgaaatt
     7981 tctggtgcac ctggatacca actacgaccg cttattgagt atcaagggca cagactgcgt
     8041 tatcgtgacg catatcaaca gcctggcaca ggagaaggcc aaacctttgg cttcaaagct
     8101 cacggaggag agccagcagt ccggggaagg tcacgatgcc tttgtcagcc cattccgcct
     8161 gattccgccc gaaattgttt tcactcaggc gctttcaatc tacctcgatg acaccgaaat
     8221 cctgctggaa caccacgcgg gttccaattt tgccgggatt tgggcggaac ttcccgccag
     8281 gaaggtcatt tttgtgggcg atactgttct cccggatcag ccgccattcc tggcgtactg
     8341 cgatttggag cgttggcttg aggacctgag ctctttatcc ggcagaaaat accgcaatta
     8401 ccagattgtt tccgcgcgca gcggggttct cgaccagagc caggttgagc aggcgagcgg
     8461 gcacatcgcc gcaatccatg cgctgctttc tccgttgata gagcgcaagg ccagtctgga
     8521 tgaggtgctc gcgcttatcc ccatcatcat gcagcgctac gaaccgaatg ccaacaccag
     8581 cgatatgtat tacaaccgcc tgcgctgggg gctgagcatg tactacgaac tcaaccacgc
     8641 ttagaacctg agggaatgcc tatgaatcaa gatttcgggt acgcaccgtt agtcgaactg
     8701 acccgcggcg ctattgtgga atgcgtccat tttggcgcat tcgcggtagt agatgtgttt
     8761 ggcaagctgc tctgttccgc gggcgaccct gacctgatta cgtttccgcg cagttccatg
     8821 aagccctttc aggtcttacc ttttgtggaa agcggaggac cggaatattt cgatctgtcc
     8881 gacgaggaac tggcaattat gtgtgcctcc cactccggga ccgactcgca tgtggacgta
     8941 ttgcgcagta tccatcaaaa agccggactg caagaagcgg atttgcagtg cggcgtgcat
     9001 tccccctctg accgggcgac gagcgaagcc atgcgcctgc gcggggaact gcccacaccc
     9061 taccgccaca attgctccgg caagcacagc ggcatgctgg ccgcagcgcg tatgcgcggg
     9121 ctgccaaaag aaaactacct ggagcttgac cacccagtgc agcagcgcat ccgcgcggtg
     9181 atggcggaga tgagcggagt gaacccggac gcgttaatcc ccgggatcga cggctgctcc
     9241 gcgccggtct atgccctgcc tctgcgcgca tttgcctggg cagcagcgcg cctttgcgac
     9301 ccgcatgagc tgcccgcgca gcggcagagc gcctgcagaa agataacaaa cgccatgcgt
     9361 acctatcctg tcatggtggc tggtcccggc aaactcgata ctgtgctgat gcaagttttg
     9421 ggggacaagc tcatttccaa gggaggagcg gaaggctatc agatgatcgg tttgctgccg
     9481 gatgctgaaa agggaagacc cggcatcggc atcgcgatga aattttcgga cggcgacccg
     9541 gatcgacgcg ccacctacag cctggtgacc gcgattttgc gtgttttcgg ttttgcggaa
     9601 cggctggaca gcgaagcctt caggcccttt gcggatgagg tagtgcgcaa ttggcgcgga
     9661 ttgacagccg gcgagaagcg catttccgct ctggccaagg cggcgctggc aggctgctgc
     9721 taagaaaggg cgcgtgaaag ctgccgtgga agtttccgca gagcaccgag agcggctgcg
     9781 cctggtgaac gaacgcctga accagttttt tggcttccca atttggcgga atccgcttcc
     9841 ggccgtggat gagctggtca gcacctttct ctcgcagaac acgaacgacc tgaaccgcga
     9901 taaagctttc gaagcaatcc ggcagcgcta taccagctgg gacgaagtgt gcgaggctga
     9961 cccggacgaa ttcatgcagg tcatcagggc ggcgggcctg gcaaaccaaa aaggtccgaa
    10021 cattcaggcc gccttgcagc gcatccggtc agagcgcggc gttattgacc tggagtggct
    10081 gcggggtctg ccattggaag aagcgcgcga atggctgctc agctttaagg gcgtggggcg
    10141 aaaaaccgcg gcaatcgtgc tgctgttttc cctggggatg ccggctttcc cggtggacac
    10201 gcatatctac agggtctcgg gcagaattgg cttgcggccg caggcaatgg acgtggacag
    10261 cacgcaccaa tacctggaag cggctgccga ccccgggcag tattatgccc tgcacttgaa
    10321 tctcatccgg ctgggcaggg aaatctgcca ggcgcgcaag cctgcctgcg cgcgctgccc
    10381 gctggtggag ctctgccagt atcccgataa ggtcctgagc tgaattagag gctgggttcc
    10441 aggctgccga ccagcgacca ggggcccgtc caggtgattt tcacctgccg ttcctgaccg
    10501 gtcaggtctt cagcggaatc cacgaacaca atccggttgg taggagtgcg cccgtaccag
    10561 cggcctttca cgcggtattc gaacaaaacc ggcacgactt ccccaagcaa tccctggttg
    10621 agctctttca ggatgctttc ctgcaggttt tccagcaggc ggaaacggcg catcttttct
    10681 tctgccggta cgtcgtcagc cagttcccgg gcagaatagg tgccgggacg gggggaatag
    10741 cgcgccagat gggcaacatc caagcgtaaa tccgccagga gctcgtaagt cttctgaaag
    10801 gctgtttcag tctcccctgg gaagccgaca atgatatcgg tagcgatgga cacacccgga
    10861 atgcgttcgc gcagttttgc caccagctcg cggtactgct ggttggtgta accgcgccgc
    10921 attgccagca gcacgccgtc atcgccggcc tgaacaggaa cctcgatatg cggcatcacc
    10981 ttgggcagtt ccgccacgct cgcaatcagt tcgtcgctca tccagttggg gtgcgacgtg
    11041 aggaagcgca ggcgtttgat ttgcgggatt tctgaaagcg aagataaaag ctgcggcagg
    11101 cgcgggctgc cgggcaggtc caagccgtaa cggtcgacga tttgtcctaa aagcacaatt
    11161 tcgctcacgc cctgaccggc gaaggtacgc gcgtcccgca caattctcag cgcgtcgcgg
    11221 ctgcgttcgc gcccgcgctt gagcgggatg acgcagtacg cgcaggcgtg tgaacagccg
    11281 agcaccaccg gcaggttttc ggaaaccgcg cgctgttcgc cggggacagc cacgccgaat
    11341 tcttcatcga gcattctgtc aatgttcatg cgccagcttt gagcgcggtc ggcttgctgc
    11401 cggtttgtga ggaagtccag cagcggttgc gggtcggaag gggcggaaaa gacatccacc
    11461 caggggaagc gtttttgcag cgcgaggttg ccgtgcacgc caaccaggca gcccatcagt
    11521 ccgatgacca gttggggatt ttgctgcttg agggatttca gggaactcag gcgcccaagc
    11581 gctttatctt cagcgctctg gcgcaccacg caggtgttca agacaatcac gtccgcgtct
    11641 tcgacattgg cagcggttac aaagcccagg ttttcgagcg aacgcgacag ccggcgggag
    11701 tccgcggtgt tcatttgaca gccttcagtc cagatgtaat atcgcataga cgcatattat
    11761 acaggtttgc cagaataccg gcgtttatag ctgttataat atttccattg gatacctcta
    11821 ttgcaaagcg taaggattgc tatggaattt acaaaaacgg attacaaacg ctgtattgtt
    11881 gtgaaagcag tcggccgcta tgacagcgcc accgccggag agctcgaaaa agttctgacc
    11941 gaactgatcc ccaccgataa agatattgtc ctggatatgt ccgatatcag ttttgtttcc
    12001 agcgcgggtt ggtggtcgat tattcgcgtg cagaaagaac tcaagaaaaa gcgccgggac
    12061 ctggtgttgg tggcgctcaa tgacaatgtg cgggattcca tggacctgat tggcatcctg
    12121 cagtatttta cggtctaccc caatatggtg gaagccctgg gaaatctcta aacccggctg
    12181 aaggaactta tccaaagagg ctggcagttc gtacctgcca gcctctcgtg tttctaccgg
    12241 atttgcagct cgtaaaccgc tttttcgcgc gtgagggggg tggtgccaga gaccaccagg
    12301 acgacctttt ccaccccgct gccaatggta aaactcaatt ccgcgcggtt tttctcatcc
    12361 agcgtgatgg tctgcacgcc gggttcatct cccagcgtta tcaggcgcag cgcgtaggat
    12421 tgcggcaggg tgttgcccag gcgcacaaaa ccggcgcttt gccagccgcc gccatcttct
    12481 tccaggtcgg aagtgtaatc gatggcgtcc agacggaagt cgtccagcgc tatgcctttg
    12541 ccgtttaccg cggcgtcggt tacataatcg aagcggatac ttaccagcga accgacaaaa
    12601 cgggacaggt ccacactttc gctgatccaa ccctgggagc tgccgttcca gccgcagccg
    12661 aacgagttcc cggaaggatt gctggtggtg caggtacggg tgttcagaat ctgccagttt
    12721 ttgccgtccg ttgaggcgga gaggaagacg tagtcgtagt ctttttccag gtcgtaccag
    12781 gtgcggaagc tcagctcagc gcggccgctc acggttgtga ggtcgaagga gcgctcgagg
    12841 gacatattgg attcgtctcc aagattggac cagaaaaagg tgctggcgct cccctcgatg
    12901 ggcaggaggc tgacagcctg cgtaccctgg aaattgaggg tgtagctgcc cgggcagc
//
LOCUS       DAOQAD010000050        12750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_5457_length_12750_cov_4.628358, whole genome shotgun sequence.
ACCESSION   DAOQAD010000050 DAOQAD010000000
VERSION     DAOQAD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12750
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5457_length_12750_cov_4.628358"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..637)
                     /locus_tag="PKL60_05405"
     CDS             complement(191..637)
                     /locus_tag="PKL60_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNW95608.1"
                     /translation="MISLLEVPGFLGTKASMLSDVSLILCLIALILFIVAGVQARQRK
                     FKQHCPTQTAALILTILAVVLFMSPVYTQKYESANAGLAPFLPNGLVAVHAGLGGLTI
                     LFGIYVTLVGYRVLRSKHKKLLMQIASILFMILNISGLIIYLNLYL"
     gene            775..856
                     /locus_tag="PKL60_05410"
     tRNA            775..856
                     /locus_tag="PKL60_05410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:809..811,aa:Leu,seq:tag)
     gene            886..2301
                     /gene="tig"
                     /locus_tag="PKL60_05415"
     CDS             886..2301
                     /gene="tig"
                     /locus_tag="PKL60_05415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNW95609.1"
                     /translation="MKLQKEFTDDQQVKVTAEFEPELLEQFMHKAARKISQSTRIPGF
                     RPGKAPYTMVLSHVGEASVREEALELLLDKVYPEILEEAQIEPQGPGNLDEVKSEDPL
                     VFVFTIPLEPEVELGPLEDLREAYSLPGISEQEVDDFILRARQNAGTIVPLETPAAEG
                     NLLYVTLEAVNLEPKEGENPDLIKAAPQQVLIPTEAEQRESEWPFQGFARALIGYNAG
                     ENAVITHDFPEDALDDELRGKKVRFTVNIQSVKALQLPEMDEEFLTELGDFKSVEELR
                     NTVLKRLETEATAEYEDKYYLDLVDRIRKEAKIKYPPQMLKEEEEQVLHRFEHELSHR
                     QLDLDVYLKMRKTTREEFIQQEVTPTAIDRLERSLVMEALSRKYDIKVTSEALEQEIS
                     ALIHDLMMSGELQEVQKELGEKKFANAVSMEAANHAMENAIRRQLRAIASPESLPAEP
                     AAELSGESALTEADVASEDAE"
     gene            2407..3012
                     /locus_tag="PKL60_05420"
     CDS             2407..3012
                     /locus_tag="PKL60_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNW95610.1"
                     /translation="MDTNFVKSVIPMVVESSGNGERAYDIYSLLLKERIIFVGSAIND
                     QVANLVVAQLLYLSREDPDKSIQMYINSPGGSIYSGLAILDTMQMIPNKISTVAVGVT
                     ASFGTVLLAGGAKGQRYALPHATIHMHQPLGGAEGQATDIEIMAHEIMRLKNALNSFF
                     ADATGQPIEVIKRDTDRDFYLTAEQAVSYGIIDKVLQPPVK"
     gene            3038..3835
                     /locus_tag="PKL60_05425"
     CDS             3038..3835
                     /locus_tag="PKL60_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNW95611.1"
                     /translation="MLTEKFPKLKGLILDMDGVLWHDTQAIGNLPALFAAISDLGLRY
                     VFATNNATKTLAEYQEKLKKFGLEVQQEQIITSAVTTAVYLQANYPSGTPIYVVGADS
                     LKQILQEHGFVICPVEEHQNAALVVVGMDIGLTYEKIRNAALLVRAGAGFIATNADAT
                     FPTPQGLFPGAGTMVAAIATASGREPQIIGKPATAMYEHAFRVLQLSPREVMGVGDRL
                     ETDIAGAQKAGCLAGLVLSGVSTRAQAQSWRPAPDRIATDLYELIHG"
     gene            3828..4871
                     /gene="rlmN"
                     /locus_tag="PKL60_05430"
     CDS             3828..4871
                     /gene="rlmN"
                     /locus_tag="PKL60_05430"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNW95612.1"
                     /translation="MAKALIHDLDFDAFSQVLTDLGQPSFRAKQLWNGLYQQLYTDFS
                     QFSNIPAGLRAALAEQYTLTPLTPRTSLRSKDQQTEKTLFGLPDELSVETVLMRYEER
                     NSLCVSTQVGCPMGCVFCATGQMGYQRGLSTGEILSQILFYMRLLQSEGKSLTNIVYM
                     GMGEPFLNYEATMASLRRLTDPSALNFGARRITVSTVGIIPKIERFSREGLQVNLAVS
                     LHAADNELRSQLVPANRLYPLKNLISACRAYTEETHRRVSFEYALINTVNDTPAAAVQ
                     LAGLLKGMLCHVNLIALNPSKHYPLQGSPREKVHAFAEALSERGIPVSVRLRRGIEIQ
                     AGCGQLASSQNPL"
     gene            4882..5790
                     /gene="ftsY"
                     /locus_tag="PKL60_05435"
     CDS             4882..5790
                     /gene="ftsY"
                     /locus_tag="PKL60_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNW95613.1"
                     /translation="MSDLLNKWRESLTKTRDIAFGKVSTFFGATQINEDSWDELEALL
                     IQADLGVETTISLINTCQEQVDRQGLTKTEQLYSLLKTELTSLLDQPEEPALSFQPTV
                     IMVAGVNGSGKTTTIAKLAARYKNMGKKVLLVAADTYRAAAVDQLEVWSKRVDVPIIT
                     AQPESDPGAVTYDGIKSALSRGSDVVLIDTAGRLHTRYNLMEEIKKVYRVAGKALPGA
                     PHYSWIVLDTTTGQNALQQAAAFAKAVQLNGALLAKLDTSAKGGMAFAIKSQLGLPVL
                     YAGLGERVEDLQLFNREAFVNGILEH"
     gene            5808..6891
                     /gene="prfB"
                     /locus_tag="PKL60_05440"
     CDS             join(5808..5873,5875..6891)
                     /gene="prfB"
                     /locus_tag="PKL60_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113918868.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNW95614.1"
                     /translation="MSDLANRIETIEAQCRSLQEHLDLAEKLQDLQALRTQSESSDFW
                     NNPDAAQQVMKRISDLTGVVEEWQALTAEIHDLKTLNEMDDGSLTGEISAQVAALEET
                     LARKETELLLSGRYDHGNALLAVHSGAGGTDSQDWAAMLERMYLRWAEQRGFKTEILD
                     RTVGEEAGIKSVTILVQGKLAYGFLKSEKGVHRLVRLSPFDAAHRRHTSFALVEVLPE
                     LEDNDEVIIRPEDINIEVYKSSGAGGQNVQKNMTAVRIVHLPTGLVVTCQNERSQTQN
                     RENALKVLRSRLFELQMQAHDEVVAELKGEFKKVEWGSQIRSYVLHPYQMVKDHRTEY
                     ETANTQAVLDGSIDGFIEAFLRMKKG"
     gene            complement(6888..7859)
                     /gene="pfkA"
                     /locus_tag="PKL60_05445"
     CDS             complement(6888..7859)
                     /gene="pfkA"
                     /locus_tag="PKL60_05445"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNW95615.1"
                     /translation="MTKRVAVLTSGGDAPGMNAAIRAVVRCGIRKGWQMYGVQHGYKG
                     LISGNINEMTTRDVGGIIQRGGTMLGSARCPEFLTVEGRKLAIRNMNQLGIDSLVVIG
                     GNGSQTGNYELHRMGFPVVGIASTIDNDLCGSDITIGVDTALNIALEAIDRLKVTASS
                     HQRAFILEVMGRKCGYIALMSGIAGGAEAITLPEIPTDPEELAQTLQSAYERGKSHAL
                     VVAAEGADYNAAKLTEFFNNNRDRLGFSVRSTILGHVQRGGTPSAYDRNLGSRFGLGA
                     IEALERGETGVLVGQIASDITTTPLEDVVGKTKGLDMELVRLAKMLD"
     gene            7946..8728
                     /locus_tag="PKL60_05450"
     CDS             7946..8728
                     /locus_tag="PKL60_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95616.1"
                     /translation="MTKDILVAIVSDDIYARNWMSLLLVRDWRTRVVSEITCHADFAR
                     LNEPGQPQPDLFLVDLDSYRDNPSMLTTLNELVGKRARILCVGTQVIPNVFLRLDAHL
                     FAGYLLKNEISSSLAWAITFAYEDKTVFTPGVMDFLQDDTYKMPANKMIIRGRSFPGL
                     TDRQEEIARLAIVFSIGRRDLADELKISDQWSYGMVSELYSRLGLEELFTGEIDPSHF
                     VWDDPVILEHLEEILDKLGDSKKARDLETLAFHLLTMPLIEA"
     gene            complement(8735..9385)
                     /locus_tag="PKL60_05455"
     CDS             complement(8735..9385)
                     /locus_tag="PKL60_05455"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNW95617.1"
                     /translation="MRILDISVGIDEKLPVWPGDPQPALSWVGRMTSGDIANISAMSM
                     GAHTGTHIDAPLHFLEDADSLDHIALSALIGEAQVLEVPEDVTLITADVLSAGEQIHC
                     ERVLLKTRNSRFWEGEGARFRQDFTALDVSAAEYLVQRGVCLVGIDYLSIAPYEDPAP
                     THTLLLQAKVVILECVNLSEAAAGRYQLVCLPMKLTGREAAPARAVLISDEMRVAI"
     assembly_gap    9687..9696
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9697..10672
                     /locus_tag="PKL60_05460"
     CDS             <9697..10672
                     /locus_tag="PKL60_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNW95618.1"
                     /translation="QLQLGALLADWLGMHHREGFGLESACSSGAAAFRAALMAIASGE
                     LSSALVVGVEKMTDSPLAEITSSLATAADADLEGDFGVSFVALNALIMQRYMHEYGWK
                     TSDFAAFSINAHANAVSNPNARFQKAISQVDYARAGMVCEPINLLDASPVSDGAAALY
                     LSAAPPAAGRKISVAASAAATDTISLQHRANPLRLSAAEESARKAYQQAGISPHEISL
                     FEYHDAFSIMAALSLEAAGFCEPGQGPRLANDGSIAVDGVIPVATMGGLKARGHPVGA
                     TGLYQLVEVALQLREQAGKTQVKAPRWGMAQNIGGSGSNIITHILHQE"
     gene            10747..>12750
                     /locus_tag="PKL60_05465"
     CDS             10747..>12750
                     /locus_tag="PKL60_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNW95619.1"
                     /translation="MMEFHVSRLSREKYGFDQLLFSLNGNVIFANFQAARKFAQQINA
                     LKSPGDAGYVSAGQINALGLIDEIFHLVVAEYYQEYGKDLRDALYTDISTRLGEEKLL
                     GTLSAFCSEFPPVQVFQGQLSLQDYLKGTTDGVANTKLALEELLMLWLTNQNPAAAVY
                     SELFDDENLRLKSAYEQVIDGVKVFFSKQPTFGPQQQDLITMLRTPAMMVPQSLSGQL
                     EFIRQHWGKLLGKYLYRLLGSLDFLKEEQRAIFAGPGPTMVPDYRSEGLGLSEIERFS
                     PDSDWMPRVVMIAKNSFVWLNQLSRQYQRPIERLDQIPDETLDRLASWGFTGLWLIGL
                     WERSDASREIKQRCGNPDAVSSAYSLARYQIAERLGGEEAYRNLSYRAGLRGIRLASD
                     MVPNHMGIDSDWVFEHPDWFISQDYSPFPGYTFNGPDLSKNPAVSINLEDHYYSRSDA
                     AVVFKRYDHGNGQTKYIYHGNDGTSMPWNDTAQLNYLDPSVREAVIQTILEVARKFPI
                     IRFDAAMTLTKKHYQRLWFPQPGTGGDIPSRAEHAMTKEAFDQAMPEEFWREVVERVA
                     KEVPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAYMNMLRNEENDKYRQLIKNTLVYDP
                     QILKRYVNFMNNPDEKTAVEQFGKGDKYFGICTVMSTMPGLPMY"
BASE COUNT         3040 a         3549 c         3432 g         2719 t
ORIGIN      
        1 gtgccaatgc agaattttgc ggtcctggta ttggagggtt tttaaagcaa ggcaaataaa
       61 agattttctt tctaaacctg aagaagcttt gaaaagatca tatgagcgcg aagtcataaa
      121 gaattctcct gaagcaatgc cttcttcttc aggagaataa tccattaagg ttcggttaag
      181 gtgatggtga tcagaggtag agattcaagt aaatgatgag accgctgata tttaaaatca
      241 tgaacaggat gctggcgatc tgcataagca gcttcttatg cttcgatcgg agaactctat
      301 aaccaaccag cgtaacataa attccaaata atatggtaag gccgcctaaa ccagcatgca
      361 ctgccactaa tccattcggt aagaatggag caaggccggc gttcgcagac tcatattttt
      421 gggtatagac tggcgacata aataaaacga ccgcaagaat agtcaggatt aaggcggcag
      481 tttgcgtggg acaatgctgc ttaaatttac gctgtctggc ttgaacgccg gctacaatga
      541 acaatattaa cgcgatcaag cacaggatca atgaaacatc tgataacatt gatgcctttg
      601 ttcctaaaaa ccctggaacc tctaatagtg agatcattta tcctccaaat acagatttat
      661 tttgaataga tatcaataat atcacgcaaa ctctggagtc tccacggtta acccaggttt
      721 tcctcagttt tggtttgact cgatatcttt cattgtatat aattgcttgt tcgaggggaa
      781 gtgctggaat tggcagacag gcatgactta ggatcatgtg cggcaacgcg tgagagttcg
      841 agcctctctt tccccacagc attcataaac agtgagaggt atattttgaa attacaaaaa
      901 gaatttacgg acgaccagca ggtcaaagtc accgcggaat ttgaaccgga gcttctcgag
      961 cagttcatgc ataaagccgc ccgcaaaatt tcccagagca cccgcatccc tggtttccgc
     1021 ccaggcaagg cgccctacac aatggtcctc agccacgtcg gcgaagcgag cgtcagagag
     1081 gaagcgctgg aactgctcct ggacaaggtt tatccggaaa tcctggaaga agcgcaaatt
     1141 gaacctcagg gtccaggcaa tctggacgaa gtcaaaagcg aggatcccct ggttttcgtg
     1201 tttaccatcc cgctggaacc tgaagtagaa cttggcccgc tggaagacct gcgcgaagcg
     1261 tactctctcc cgggaatcag tgaacaggaa gttgacgact tcattctgcg cgcgcggcag
     1321 aacgccggca caatcgtccc gttggaaact ccggctgcgg aaggaaacct gctgtatgtg
     1381 accctggaag ccgtcaacct tgaaccgaag gaaggcgaaa atcccgacct tattaaggcg
     1441 gctccgcagc aagtgctcat ccccactgaa gctgaacaac gcgagagcga atggcctttc
     1501 cagggtttcg cccgcgctct catcggctac aatgctggcg agaatgccgt catcacacac
     1561 gattttccgg aagatgccct ggatgatgag ctgcgcggca agaaggttcg cttcacagtc
     1621 aatatccaat ctgtaaaagc attgcagctg ccagaaatgg acgaggaatt cctcaccgaa
     1681 cttggcgact tcaaatctgt cgaggagctc cgcaacacgg tattgaaacg cctggaaacc
     1741 gaagcaactg cagaatatga agataagtat tatctcgatt tggtggatcg catccgcaag
     1801 gaagcgaaaa tcaaataccc ccctcagatg ctgaaggagg aagaagagca ggttctgcac
     1861 cgtttcgagc atgagctctc ccatcgccag cttgacctcg atgtttatct gaagatgcgc
     1921 aagacaacgc gagaagaatt catacagcag gaagtcaccc ccaccgcgat tgatcggtta
     1981 gaacgctccc tcgtgatgga ggccttgagc aggaaatacg atattaaagt gacctctgaa
     2041 gccctggaac aagaaatttc ggcccttata catgacctga tgatgagcgg cgagctgcag
     2101 gaggtacaga aagagctggg cgagaaaaaa ttcgccaatg ctgtctcgat ggaagctgct
     2161 aaccacgcta tggaaaacgc cattcgcagg cagctgcgcg caatcgccag cccggaatcc
     2221 cttccggctg aacccgcagc ggaactcagc ggagaaagcg cgctgacaga agcagacgta
     2281 gcatctgaag atgcagagta attacccaaa atgggcgcct gaacatccag gcgccctctt
     2341 tctataacaa atatctaata gttgtcagct aaaatcttca aaatatctat gaagaaaagg
     2401 aaacctatgg atacaaactt cgtaaaatca gtgattccga tggtcgtgga atcttccggc
     2461 aacggggaac gcgcctatga tatttactcg ctcctgctca aggaacgcat catcttcgtc
     2521 ggttccgcca tcaacgacca ggtagccaat ctggtcgtcg cccagctgct gtatctcagc
     2581 cgcgaggacc ccgacaagtc aatccaaatg tacatcaact cccccggcgg ctcgatttac
     2641 tccggtctgg cgattctcga cacgatgcaa atgatcccga acaagatcag tacggttgcg
     2701 gtcggcgtta cagccagctt cgggaccgta ctgctggccg gcggcgcaaa gggtcagcgc
     2761 tacgcgctcc cgcatgcgac catacacatg catcagcctt taggcggtgc ggagggccag
     2821 gcgacggata tcgagattat ggcgcacgaa atcatgcggc tgaagaacgc cctaaacagt
     2881 ttttttgcgg atgcaaccgg tcagcctatc gaggttatca agcgcgatac tgaccgcgac
     2941 ttctacctga ccgcggaaca ggctgtcagt tacggcatca tcgataaagt gcttcaacct
     3001 ccggttaaat aatttactct gccttgtcgg aggacgaatg ctgacagaaa agttccccaa
     3061 actcaagggg cttatcctgg acatggacgg tgtcctctgg cacgataccc aagccatcgg
     3121 aaatctgccc gctttatttg cggcgatttc cgatttgggc ttgcgttatg ttttcgcgac
     3181 gaacaacgcc acgaaaacgc tggcggaata ccaggaaaag ctgaagaaat tcgggctgga
     3241 agtgcagcag gagcaaatca tcacctctgc tgtgaccacc gcagtgtatc tgcaggcgaa
     3301 ttacccgtct ggtacgccca tctacgtggt gggcgcggat tccctcaagc aaattctgca
     3361 ggaacatggc tttgttatct gcccggtcga ggagcaccaa aacgctgcac tcgttgtggt
     3421 cgggatggac atcgggttga cgtatgaaaa gatccgcaac gccgcactgc tggtaagggc
     3481 aggcgccggc ttcatcgcca ccaacgcgga tgccaccttc cccaccccac agggtttgtt
     3541 tcccggcgcc gggacgatgg tggcagcgat tgccactgct tccggccggg agccgcaaat
     3601 tatcggcaaa cctgccaccg cgatgtacga gcatgccttc cgcgtcctgc agttgagccc
     3661 ccgggaagtg atgggggtcg gcgaccgtct ggaaaccgat attgccggcg cgcagaaagc
     3721 aggctgcctg gcaggcctgg tcctttcggg ggtttccacg cgcgcccagg cgcaatcctg
     3781 gcggcccgcg ccggatagga ttgccacaga cctttatgag ctgattcatg gctaaagcgc
     3841 ttatccacga cctggatttc gatgcctttt ctcaggttct taccgatctt gggcagccat
     3901 ctttccgcgc caaacagctc tggaacggcc tttaccagca gctttatacg gatttttctc
     3961 agttttccaa catcccggcc ggcctgcgcg ccgcgctcgc ggaacaatac acgcttaccc
     4021 ctttgacccc gcgcaccagc ttgcgctcca aagaccaaca gacggaaaaa actttgtttg
     4081 gtctgcccga cgagctgagc gtcgaaactg tgctcatgcg ctacgaagag cgcaattcgc
     4141 tgtgcgtttc cacccaggtt ggctgcccga tgggctgcgt tttttgcgcg acgggacaga
     4201 tggggtacca acgcggttta tccaccggtg aaattctctc ccaaatcctg ttctatatgc
     4261 gccttttgca gtccgagggg aaaagcctga ccaacatcgt ctacatgggc atgggagaac
     4321 ctttcctgaa ttacgaagcg accatggcgt ctctgcgcag gttaacagac ccaagcgcgc
     4381 tcaactttgg agcacggcgc atcacggtca gcacagtagg catcattccg aaaatcgaga
     4441 gattcagccg cgaagggctg caggtgaatt tggcggtcag cctgcacgct gcggataacg
     4501 agctgcgcag ccagcttgta cccgccaatc ggctctatcc gctgaagaac ctgatttccg
     4561 cctgccgcgc ctacacggaa gaaacccatc ggcgcgtgag ttttgaatac gcattgatta
     4621 acaccgtgaa cgatacgcca gcagcggcag tccaactggc gggtttgctc aaaggcatgc
     4681 tttgccacgt caacctgatc gcgttgaacc ccagcaagca ttatccgctg cagggctccc
     4741 cgcgcgaaaa ggtgcatgct ttcgcggagg cgctttctga acgcggaatt cctgtgtcgg
     4801 ttcgcctgcg ccgcggcatt gaaatccaag ccggctgcgg tcaactggca agcagccaaa
     4861 acccgttata atactcagcc tatgagcgat ttactgaata aatggcgtga aagcctgacg
     4921 aaaacccgcg acatcgcctt tgggaaggtt tccactttct ttggcgctac ccaaatcaat
     4981 gaggacagct gggacgagct ggaagccctg ctcattcagg cggacctggg tgtggaaact
     5041 accatctcct tgattaatac ctgccaggag caggtagacc ggcagggctt gaccaagacg
     5101 gaacaacttt acagcttgtt gaaaaccgaa ctgaccagcc tgttggacca gccggaggaa
     5161 ccagcgctaa gcttccagcc tacggtcatc atggtagccg gcgtaaatgg atccgggaaa
     5221 acgacaacca ttgccaagct ggcagcccgc tacaagaaca tgggcaaaaa agtgctgctg
     5281 gtcgccgcag atacctaccg tgccgccgca gtggaccagc ttgaggtttg gagcaaacgc
     5341 gtggacgtgc ccatcatcac agcccagccc gagagtgacc cgggagccgt gacctatgat
     5401 gggattaaat ccgccctctc gcgcggcagc gacgtggtgc tgattgacac agccggccgc
     5461 ctgcacaccc gctacaacct gatggaagaa atcaagaagg tctatcgcgt ggcaggcaaa
     5521 gcgctgcccg gcgcgccgca ctattcctgg attgtgttgg acaccactac cggccagaac
     5581 gcgctccaac aggcagcggc ttttgccaaa gccgtgcagt tgaacggagc tctcctggcc
     5641 aagctggaca catccgccaa aggcgggatg gcgtttgcca tcaaaagtca attgggcttg
     5701 cccgttctgt atgcaggact gggagagcgc gtggaagatt tacagctttt caacagggaa
     5761 gccttcgtga acggcatcct tgagcattaa ctttccggag aaaaaccatg tctgaccttg
     5821 ccaaccgcat agaaactatc gaagcgcagt gccgctccct ccaggagcat ctttgacctc
     5881 gcggaaaagc tacaggacct gcaggccctc cggactcaat ctgaaagctc cgatttctgg
     5941 aataaccccg atgccgctca acaggtgatg aagcgcatct ccgaccttac cggtgtcgta
     6001 gaggaatggc aggcgctgac cgcggaaatc catgacctca aaacgctcaa tgagatggac
     6061 gacggctcgc tcaccgggga gattagcgcg caggtcgctg ccttggaaga gaccctggca
     6121 agaaaggaaa cagagctgct gctttctggc aggtacgatc acggcaacgc tctgctggcc
     6181 gtccactccg gcgcgggcgg caccgattcc caggattggg ctgccatgct cgagcgcatg
     6241 tatctgcgct gggcggaaca acgcggtttt aagactgaaa tcctcgaccg caccgtcggg
     6301 gaagaagccg ggatcaaatc ggtgaccatc ctggttcagg gcaagctggc ttacggattc
     6361 ctgaaatctg aaaaaggcgt ccaccggctt gtgcgcctct ccccgttcga cgcagcacac
     6421 cgccggcata cctccttcgc attggtggaa gtgctgccgg agcttgagga caatgatgaa
     6481 gtgattatcc gtccggaaga tatcaatatc gaggtctaca aatcctccgg cgctggcggg
     6541 cagaacgtac agaaaaacat gaccgcggtg cgcattgtgc atttgccgac cggattggtt
     6601 gtaacctgcc agaacgaacg ctcacagacc cagaaccgcg aaaacgcgct caaggtgctg
     6661 cgctcccggt tgttcgagct gcagatgcag gcacacgatg aggttgtggc ggagcttaag
     6721 ggtgaattca aaaaggtcga gtggggcagc cagatccgtt cttacgtgct ccatccctac
     6781 caaatggtca aagaccaccg cacggaatac gaaaccgcca atactcaagc agtactcgac
     6841 gggagtattg acggttttat tgaagcgttt ctgaggatga aaaagggtta atcgagcatc
     6901 ttcgccagac gcaccagttc catatccaga cctttggttt tcccgaccac gtcctcaagc
     6961 ggagtggtcg taatatctga agcaatctga ccaaccagaa ctccggtttc gccgcgttcc
     7021 agggcttcga tggcgcccag gccgaagcgg ctgcctaaat tccgatcata agcgctgggt
     7081 gtgccgccgc gctgaacatg cccgagaatg gtgctgcgca cgctgaatcc caggcggtca
     7141 cgattgttgt taaagaattc agtgagtttg gcagcattat agtcagcccc ttcagcggct
     7201 acgaccaacg cgtgcgattt accccgctcg tacgctgatt gcagagtctg cgccaattct
     7261 tccgggtcgg tggggatttc cggcagggtg atggcttccg ccccgccagc aatgccggac
     7321 atcagggcga tatagccgca cttccgcccc atgacctcca ggatgaatgc gcgttggtgg
     7381 gaggaggcgg tcactttcag gcggtcaata gcctccaggg cgatattcag ggcggtatcc
     7441 acgccgatgg tgatatctga gccgcacaag tcgttatcga tggtggaggc gattccaacc
     7501 accggaaaac ccatccggtg cagctcgtaa ttcccggttt gggaaccgtt cccgccgatg
     7561 acgaccagcg aatcgatgcc gagttggttc atgtttctga tagccagttt acggccttct
     7621 acggtcagaa actcggggca gcgcgcgctg ccaagcatcg tcccaccgcg ctgaataatc
     7681 ccgcctacgt ctcgtgtggt catctcgtta atattcccgg aaatcagtcc tttatatcca
     7741 tgctgtacgc cgtacatctg ccagcccttc cggatcccac agcgcacaac ggcgcggata
     7801 gccgcgttca ttccaggggc gtcgcctccg cttgtaagta ctgcaaccct tttcgtcata
     7861 tgtcacctct ttactttgga ccgataatta cttttcgtaa ttataattca tgtcaatcgt
     7921 agaaattgga aggaatccgg tcaaaatgac taaagatatt ttggtcgcta tcgtcagtga
     7981 tgatatttac gcgcggaatt ggatgtctct gctgctggtg cgcgattggc gaacccgggt
     8041 ggtttccgaa atcacatgcc atgccgactt tgccaggtta aatgaacctg gtcaacccca
     8101 gcctgacctt ttcctggtgg atttggactc ttatcgggat aaccccagta tgctgaccac
     8161 gctgaatgaa ctggtcggta agcgcgccag aattttgtgc gtaggtacgc aggtgattcc
     8221 caatgtattt ctgcgcctgg atgcgcacct ttttgccggc taccttctca agaatgaaat
     8281 ctcgtcatcg ctggcctggg ctatcacttt cgcctatgag gacaagactg tatttacccc
     8341 cggcgtgatg gacttcctgc aggatgacac ttacaaaatg cccgcgaaca aaatgattat
     8401 ccgcggccgc agctttccag gcctcacgga ccgtcaggaa gagattgcgc ggctcgcgat
     8461 tgtgttctcg attggtcggc gggatctggc ggatgaattg aaaatttcgg accaatggag
     8521 ctacggcatg gtaagcgagc tttacagccg ccttggactg gaggaactct tcaccggtga
     8581 gatcgaccct tcccactttg tctgggatga cccggtaatc cttgagcacc tggaggaaat
     8641 cctggataag ttgggagact ccaaaaaggc gcgcgacctg gaaaccctgg cgtttcacct
     8701 tttaactatg cccttgattg aagcttaaga ataattatat ggctacgcgc atttcatcgg
     8761 aaataagcac agcccgggcc ggggcggctt cgcgtcctgt caatttcatc ggcaggcaga
     8821 ccaattgata ccggcccgcc gccgcttcgc tcaaattgac gcactccagg ataaccactt
     8881 tcgcctgcaa gagcagtgta tgcgttggcg ccgggtcctc ataaggggca atggagagat
     8941 aatcaatccc taccaggcac actccgcgct gcacaagata ctctgccgct gaaacatcaa
     9001 gcgcagtgaa atcctgccgg aaacgagcgc cttcaccctc ccagaagcgg ctgttcctgg
     9061 ttttcaacag cacgcgttcg cagtggattt gctctcccgc gcttaaaaca tctgcggtaa
     9121 tcagggtcac atcctcagga acttcgagca cctgagcctc ccctatcagc gcggacaacg
     9181 caatgtggtc caggctgtca gcatcctcga gaaaatgcag cggtgcatcg atgtgcgtgc
     9241 cagtatgcgc gcccatgctc atcgcgctga tattcgcgat atccccagag gtcatacgtc
     9301 cgacccagct gagcgcgggc tgcgggtcac ccggccagac aggcaatttt tcatcaattc
     9361 caacgctgat atcgagaatt ctcatgcgtc ctccaatgct ccaatggatg ctgttttctg
     9421 gaattataca gacagaaacg ggttttgaga gttttcgaga ggaaatgggt atagaatctg
     9481 cgctgaaaac cagaatatca agaggtcgag atgcaaaaga tttatatcgc cggcgtcggg
     9541 cagacacctg tccgggagaa ttgggaaaag tcgctcaaag aactggcagg tgacgccgcg
     9601 ctcctggcgc tggaggatgc caggctcgag tatccagagg gaatcttcgt gggaaacatg
     9661 atgtccggca gcgctaatcg ccagctnnnn nnnnnnccag cttcaactgg gcgcgttgtt
     9721 ggccgattgg ctgggaatgc atcaccgcga gggcttcggt cttgagtctg cctgcagttc
     9781 cggcgcggca gctttccggg cggctttgat ggccatcgca tccggtgagc tctcctccgc
     9841 gctggttgtg ggcgtggaga agatgacgga ctcccccctg gcggaaatta ccagcagcct
     9901 cgctacagcc gccgacgcgg acctggaggg ggattttggc gtgtcttttg tcgccttgaa
     9961 cgctttgatt atgcagcggt acatgcacga atacggctgg aaaacctccg atttcgcggc
    10021 cttctccatc aacgcgcacg ccaatgcggt ctccaacccc aatgcccgct tccaaaaggc
    10081 tatcagccag gtggattatg cacgcgccgg catggtatgc gagcctatta acctgctgga
    10141 tgcatccccg gtcagcgacg gagccgcggc tctgtatctt tcggctgccc cacccgctgc
    10201 cggcagaaaa atcagcgtgg ctgcctccgc cgccgcaacc gatacgattt ccctgcagca
    10261 ccgcgcaaat ccgctgcgcc tgtccgctgc tgaggaatcc gcccggaaag cttaccagca
    10321 ggctggcatc tccccacacg aaatcagctt gtttgaatat cacgacgcct tttcaatcat
    10381 ggcagccctt tcgctggagg cagcgggttt ttgcgagcct ggacaaggtc cgcgccttgc
    10441 caacgacggc agcatcgcgg tcgacggcgt aattccggtg gcaaccatgg gggggctcaa
    10501 agcccgcggt cacccggtcg gcgcaacggg gctctaccag cttgtggaag ttgccctcca
    10561 gctgcgtgaa caggcgggga agacccaggt gaaagctccc cgttggggga tggcgcagaa
    10621 tatcggcggg tcgggttcaa atatcattac ccacatcctg catcaggaat gatacaattt
    10681 cccgggtaaa caaattgccg cagcacttca gcggcgcctt gcgcgttcaa caaaggagag
    10741 atttccatga tggaattcca tgtttcacgc ttgtcccgtg agaagtacgg ctttgaccag
    10801 ctgctttttt cgctgaatgg caacgtcatt ttcgcgaatt ttcaggcagc ccgcaagttt
    10861 gcgcagcaaa ttaacgcgtt gaaaagccct ggcgatgccg gttatgtgtc agcgggtcaa
    10921 atcaacgcgc tgggtctgat tgacgagatt ttccaccttg tcgtcgccga gtattatcag
    10981 gaatacggca aagacctgcg cgacgcgctg tacacggata tctcaacccg gctgggcgag
    11041 gagaaactgc tcggtacact cagcgctttt tgcagcgagt tcccgccagt ccaggttttt
    11101 cagggtcagc tcagcctgca ggactacctc aagggtacga ctgacggcgt cgcgaacaca
    11161 aaactcgcgt tggaagagct gctgatgctc tggctgacca accagaaccc tgccgcggcg
    11221 gtttattcag aacttttcga cgatgaaaac ctgcgtctta aaagcgctta cgaacaggtg
    11281 attgacggcg taaaagtttt tttcagcaag cagccgacct tcggccccca gcagcaggac
    11341 cttatcacca tgctgcgcac gccggccatg atggtgccgc agtcgctcag cggccagctg
    11401 gaattcatcc gccagcattg gggcaagctg ctcgggaaat acctgtaccg cctcttaggc
    11461 agcctggact tcctgaaaga ggaacagcgc gcgatttttg ccggccccgg ccctacaatg
    11521 gtgccggatt accgttcaga agggcttggc ctttccgaga tcgaacgctt tagccccgac
    11581 agcgactgga tgccgcgggt tgtcatgatc gccaagaaca gctttgtgtg gttgaaccag
    11641 ctttccaggc agtaccagcg cccgattgag cgcctggacc aaatccccga tgaaacgctg
    11701 gacagactgg catcctgggg cttcaccggc ctatggctga taggcttgtg ggaacgcagt
    11761 gacgcctccc gtgaaatcaa gcagcgctgc ggcaatccag acgctgtttc ctcggcttat
    11821 tctctggcac gctatcaaat tgcagagcgc ctgggaggag aagaagccta ccgcaacctg
    11881 agctaccgcg cgggtttgcg cggtatacgc ctggcaagcg atatggtgcc caaccatatg
    11941 gggattgatt cagactgggt ttttgaacat cctgactggt ttatttctca ggactacagc
    12001 cccttccccg ggtacacctt caacgggcct gacctctcga agaaccccgc ggtcagcata
    12061 aacctggaag accactacta ctcccgcagt gacgcggcag ttgtgttcaa gcgctacgat
    12121 cacgggaacg gacaaacaaa atacatttat cacggcaacg acggcaccag catgccctgg
    12181 aacgacaccg ctcagttgaa ttatctcgat ccgagcgtgc gcgaagcggt catccaaacc
    12241 atcctggaag ttgcgcggaa attccccatc atccgctttg acgcggcgat gaccctgacc
    12301 aaaaaacatt accagcgcct gtggttcccg cagccaggca ccggcggcga tatcccctcg
    12361 cgcgcggaac acgccatgac caaagaggca ttcgaccagg ctatgcccga ggagttctgg
    12421 cgtgaagtgg tggaaagggt cgccaaagag gtcccggata cgctgctgct ggcagaggcg
    12481 ttctggctga tggaaggtta ctttgtgcgc acgctcggga tgcatcgcgt ttacaacagc
    12541 gcgtacatga acatgctgcg caacgaggaa aacgacaaat atcgccagct tatcaagaac
    12601 acgctcgtct acgacccgca aattctgaag cgttatgtaa atttcatgaa taaccccgac
    12661 gagaagaccg ctgtggaaca gttcgggaaa ggggataaat acttcggcat ttgcaccgtg
    12721 atgtccacga tgcccggtct gccgatgtac
//
LOCUS       DAOQAD010000051        12394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_5708_length_12394_cov_5.178215, whole genome shotgun sequence.
ACCESSION   DAOQAD010000051 DAOQAD010000000
VERSION     DAOQAD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12394
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5708_length_12394_cov_5.178215"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /locus_tag="PKL60_05470"
     CDS             <1..1743
                     /locus_tag="PKL60_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNW95620.1"
                     /translation="RAAGIFPAFSAGNSGSSCGTIGSPGDYQESFASANHMSGRTINS
                     LSSRGPSTYGHEPYTKPNISAPGTSICSSIPGSSWSCGYSGTSMASPHSAGAVALLWS
                     CNSALVGQIDQTFQLLQNSADASIDGGNCSAPPDGQGNYTYGYGYLNVLAAGTLTCGT
                     VEYGTLQGHVYDGYGSPIEGATVTAVTSATSASDGSYSMSVPVGTYSVTASKYGYESQ
                     TVPGVVISEGITTTQDFSLTYIGAWEEGTAPCFDWTRYDVEYFPATGLIYALGGRSDT
                     STIGDIYSYNPVTGTCADTGANMPVPISNYTVNLVNNGTADVLCTFGGRNSAGSSVLN
                     VQCYNPLTNTATSVATLPSAWTGFTPGAQVVYNNKVYIFGGFNSLASPYETARTERFD
                     PTTNTFTQLGNLSLARGYLYAAEVDGKIYAFGGTVFDGTNLNAQTRAEVMAAPEGAGT
                     WSDAAVADLPAAYDEGQTYGFDSGFDIPSVAGKIIFAGGGQWPGETADVWTYDTATNT
                     YDTSFPDLQHARRNHGGVFVPIETAGMDDGLPGLWVFGGRLGADTPPYQGVEFFPLAY
                     PPVVYKEYLPILVK"
     gene            2147..8410
                     /locus_tag="PKL60_05475"
     CDS             2147..8410
                     /locus_tag="PKL60_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNW95621.1"
                     /translation="MHKKSTYRVLALLLAVGMLFSMLNTTTVRAENTEPQAGIPVAPE
                     VLTEMEAKGTASYWIEFTAKTDLSPAYNMGWSERGWYVYNTLSQAAKTSQARVAAYLE
                     NSRVEYQSYWIKNTILVKSSNLTTLNGLLGFSEISAITPRQTFILYEPDTSAAVIDNG
                     VKAVEPNLTHVNADDVWAMGIDGTGLVVANVDTGVRFTHQALVNQYRGNLGGGSFDHN
                     YNWFNPDDLTDNAPRDGNGHGTHTMGTMVGDDGGSNQIGIAPGAKWMACAGCPDGSCT
                     DSALLGCGQFIAAPTDLTGGNADPDMRPNAVNNSWGDCSQTYDSWYASPIAAWQAAGV
                     YPIFSNGNASNCGYSAPPGLNTVGNPARSGNVTGVGSSGEQNGQYATHSNWGPTDNAD
                     TVNPTGGFVYMKPQVLAPGVSIRSSTPGSDTEYQDGWSGTSMSAPHVTGLVALIWQAA
                     PCLVGDYATTENIIEETAVNITYADGSTDTPTDWPNYATGWGEIDALAAVTQASGLCA
                     MGTLEGVVTEDGTTPIEGAQILADNGSGYTKKIYSAADGSYSSGLPEGTYTLTASKYG
                     YDTVSVSDVDVTEDVTTTQDFVLEPLGTSLVSGVVYDAGVEGLGAHGYPLYAAIHVTT
                     GSFDQTIYSDPFTGYYEIELVSDTEHSFTTTPLAPGYEEKVEAVTPSGANYTHDIPLV
                     VEGEACAAPGYKPDYDFFYSFESSDEGFTPGGTTSFAWGDFTSGPGEGHSGTKGIATN
                     PGGNYNPSELGWMASPVLDMTGYGTNSIAIQWWDWKDIESASYDWARLDVTKDGGATW
                     DVVWGPVGGVSDTSYSQQTVLLDPSYNVANFQFRFYFKSDSSVQYEGWYVDDVGVVAI
                     PVPPPTTVFSSNFDSDNGGFTVSGTTTWAWGAPTSGPGAAHSAPNVWATNLAGNYNSS
                     ESGWITSPVIDLSAYAGLAPTLSFWNWMDTESTSFDWGVLEVTKDGGVSWQDVSGKIG
                     DQLSWAKKVYQLDSSYAVSNFQFRFYFRSDSSVNYAGWYIDDVVVTVAEPVSVSAPCT
                     VIPGGAVAGFVYNDNDGSPLVGADVYSAEVATQTISIPEDPANEGLYWAFQPFTEEGA
                     QGTQSLTGSNVVFDPAAGGDAGYTPGVPATLCFTAHSYSPDWEYVYDLWTRFPTDWTV
                     TDVYVQGTPYCAYGAFSDFSWSFETSPYEVDIEHQRSQFNYPDENCVATYCMEVTPGS
                     SAGNVSWYWDGDNWGNAPHHPCSSDVYTPASMVAVPCDEWVNPQASVPLLGSFEAHDF
                     TAEMALFGSQTETVNVMEDFITRQDFSLGTGELEFDPESFEVTMMMGDPVHEETLTIG
                     NNGSSPAMFELVEKDSGFVPPLSIPAFTEVLPEDTRTISMGKAPEAAKTVGLEQAPGS
                     LTDILAGAPAFAIDIYPGYNLVNIPDTDVPGVWNIVGSMGSHDFFAGDFVGGDFTTLY
                     AVDYNTNGLYAINTATGAQTLIGPTAPPAGQTFSGLTGTPDGTMYGLVTSCSASSLVT
                     VDIVTGATTLLGALPGIGCGIDLAYNTADDMIYIVELLTSNLMKVDPATLAVTTVGSL
                     GVAPNYAQGMDFEEESGVLYWAAYISSGELRVIDTTTGASTLVGAFPSGAETDCLAFP
                     TGGVSDVPWLSEDPVSGTVPAGGEQEVTISFDPTGAGLSQPGDYLAELKVKHDTPYTY
                     PNIPVILHLFAPDTFGTVNGTVSGLEACDVNPEALEGATVNFWQGGAIVYTTSTNASG
                     YYTYTVPAGTYDIEFVADGYVSIMESDVAVVGGSTLTIDKALRLLAPCLSVLPTELEQ
                     TQLADVVTTQTLTIVNSGAAAGIFELQEIPVIQQEALGPTYTYRPELDGPALDRSKSG
                     PKNSANAPTYQPKDILLDEGFESGFPPADWAVQSQSPWTWELSDYSPHSGLYNAHVLY
                     DQDLNEQDEWLVTPEFNLNEGTLSFWSMGSIYWCRDTYDNCDLNIWLVVDDIGGGDDI
                     FIRAADSDWTASWTWANTTIDLGSYIPGGPVRIGFQYAGLDGAEIGLDDVTIDGTEGG
                     DVPWLSEDPIAGTVPADGSQVITITYDSTGLAEGDYFATLRVKNPPAVAINIPVTLHI
                     TSVLQLYIPLLFK"
     gene            8525..10099
                     /locus_tag="PKL60_05480"
     CDS             8525..10099
                     /locus_tag="PKL60_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNW95622.1"
                     /translation="MMKHLPLYGSILIIALLFVSCSAPAAPQASPTAAPDAQNCLPDV
                     WVAPEVPKRIRDNFVLPASLQWAQNRGPCSVTLGLESLRAPDEVVFAQILWEYALVAP
                     SWSFREGAGSADLKAAWGGKARGSMMQIKQIIVYAEDYNSLLGLLGEPAEDGSLSEII
                     SLAPNEKISLSDQLGASVWGIVPLDRIQADYRVLAIDEVSPLQADFDRQEYALRGIYS
                     LHASPETAARLGDAGIAALTRGLAGEEADAQNSQTQAATETPAQPESRQTPTVSVGER
                     VRQKDGAVEALIPAGVFTSGCIREHNGGYDCPDDELPSRRVNLDAFYIDKYEVTNRQY
                     ALCAAAGACPEPVYRYSASRVSYYGNPAYDDYPAANISWTEAAAYCAWAGGRLPTEAE
                     WEKAARGTDLRAFPWGDSNPSCAKANAKDELLGSNCVGDTLPVGSLAEGASPYGVMDM
                     AGNVWEWVQDWYVLDENGLRIVVDPFAQDLGFHKVVKGGSWDYSWSRLRIAYNSDHEA
                     DAHKISFGFRCAAPVD"
     gene            complement(10261..>12394)
                     /locus_tag="PKL60_05485"
     CDS             complement(10261..>12394)
                     /locus_tag="PKL60_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW95623.1"
                     /translation="TSQNPEATFQALEKFGRDLTALARQGKLDPVIGRDEEIRRTIEI
                     LSRRTKNNPALIGDPGVGKTAIVEGLAQRIVKRDVPEGLKKKQVVQLDMAALVAGAKY
                     RGEFEERLKAALKEVTDAEGAIILFIDELHTVIGAGAAEGAMDAGNILKPMLARGELH
                     LIGATTTSEYRKYIEKDAALERRFQPVMVLEPSVEDTISILRGLKSRYEVHHGVRITD
                     PAVIAAATLSSRYITDRKLPDKAIDLIDEAAAHLRAEIDSKPQALDEVDRQIMQLEIE
                     RQALQKEKDQASRDRLAKIEEDLTDLREKSSILNLRWSTEKDAISNLRTLKAQLEETE
                     QEIEQAERDANLEKAARLRYETLHQLNEKIQTAEAKLKEIQQNGALLKEEVDSEEIAE
                     VVAKWTGIPVTNLLEGEMQKLLHMEDRLHQRVVGQDEAVRAVSNAVRRSRSGMQDPNR
                     PIGSFIFLGPTGVGKTELARALAEFLFVDQNAIVRVDMSEYQEKHTISRLFGAPPGYV
                     GYEEGGQLTEAVRKRPYSVVLFDEIEKAHPEVFNALLQVLDDGRMTDGQGRTVDFRNT
                     LIIMTSNLGSQLWSDPEKARHVTRDQVTEILKNFFRPEFLNRVDEIVIFKPLEKEDLE
                     KIVGIQLRHVAELLAARGYTLKSTPAASAWLAEIGFEPEFGARPLKRAIQREVQDPLA
                     LKILSGEFHEGDTIILDVGPDGLIFRNS"
BASE COUNT         2513 a         3571 c         3590 g         2720 t
ORIGIN      
        1 cgggctgccg gtatcttccc ggctttctcg gcgggcaaca gcggatccag ctgcggcaca
       61 attggcagcc cgggcgatta ccaggagagc ttcgccagcg ccaaccacat gagcggcagg
      121 acaatcaaca gcctatccag ccgcgggcct tccacgtatg gacatgagcc ctatacaaaa
      181 cccaatattt ccgcgcccgg caccagcatc tgctcatcca ttcccgggag cagctggagc
      241 tgcggctaca gcggcacatc catggcttcg ccacacagcg cgggcgccgt ggcgctgctc
      301 tggtcgtgca acagcgctct ggtagggcaa attgatcaga ctttccagct tttgcagaac
      361 agcgcggacg cgtccatcga tgggggcaac tgcagtgctc ctcctgatgg gcagggcaac
      421 tacacctacg gttacggcta cctgaatgtc ctcgcggcgg gcacgcttac ctgcggcacc
      481 gtggaatacg gcactctgca aggtcatgta tatgatggct acggcagccc gattgagggt
      541 gcgaccgtga ccgccgtgac ctcagcgaca tccgcctcgg atggctcgta ttctatgagc
      601 gtcccggtcg ggacctattc cgtcacggcc agcaagtacg gttacgaatc gcaaaccgta
      661 ccgggcgtgg tcatttccga aggcatcacc accacgcagg actttagttt gacttacata
      721 ggcgcatggg aggaagggac ggcgccctgc tttgactgga cgcgctatga cgtcgaatac
      781 ttccccgcca cgggcttgat ttacgcgctg ggcggccgct ccgacaccag cactatcggc
      841 gatatctaca gctacaaccc ggtcaccggc acatgcgctg ataccggcgc gaatatgcca
      901 gtgcctatct ccaactacac ggtgaacctg gtgaacaacg ggacagccga tgtcttgtgc
      961 acctttggcg ggcgaaactc tgctggttcc agtgtactta acgtgcagtg ctacaacccg
     1021 ctcaccaaca ccgccacatc ggttgcgacc ctgccaagcg cctggacggg tttcactccc
     1081 ggcgcgcagg tggtctacaa caacaaggtc tacatttttg ggggtttcaa ctctctcgct
     1141 tccccctatg agaccgcacg gacggagcgc tttgacccga ccacaaacac ttttacccag
     1201 cttggcaacc tgagcctggc gcgcggttac ctgtacgctg cggaggtaga cggcaagatt
     1261 tatgcgtttg gaggaacagt ctttgatggc acaaacctga acgcgcaaac ccgcgccgag
     1321 gtgatggcgg ctccggaagg ggctggcacc tggagtgatg ccgcggtagc ggatttgccc
     1381 gcggcctacg atgaaggcca aacctacggt tttgacagtg gcttcgatat ccccagcgtg
     1441 gccggaaaga ttatctttgc cggcggcggg cagtggcctg gcgagactgc ggatgtgtgg
     1501 acttacgaca ctgcgaccaa cacctacgat accagcttcc cggatttgca gcacgcgcgc
     1561 cgcaaccacg gcggcgtctt tgtgccgatt gagacagctg gcatggatga tggtctgccc
     1621 ggtttgtggg tgtttggcgg ccgtttgggc gcggataccc cgccctatca gggcgtcgaa
     1681 ttcttcccgc tggcttaccc gccggtcgtt tacaaagagt atctcccgat actggtaaag
     1741 taagccgaag tttcgtttct gagcctgcgc tcctggaagc ttttttcagg agcgcttttt
     1801 tgcgtgtgag cggccgggct gtttcgccct ctccagtcag gatataaccg ggaaaaagcc
     1861 ctgaaaaact gataaacccc cttgtcgcca cccgaagtgg tgatacaatg ctatctgata
     1921 aattattaat tttacattcc atgtattaat agtgttgtaa gttgcgcgca aagttcagct
     1981 taccgttcct tcaggctcca atctttttat ctccaaaatc agttgttcac tttctttacc
     2041 aaggaggtgc cgaacataga ataagtaatc ctggtttccg cttacctgtt tcgtttgtgt
     2101 tttcccgtcg ttttaagctt gttttcaata ctggaaggag caagaaatgc acaaaaagtc
     2161 aacttacaga gtgttagccc tgttgctggc tgttggcatg ctcttcagca tgctcaacac
     2221 caccaccgtc agggcggaaa acaccgagcc gcaggcagga attcctgtgg ctccggaagt
     2281 gttgacggag atggaagcca aaggcacagc cagctactgg attgagttca ctgcaaagac
     2341 ggacctttcc ccggcgtata acatgggctg gtctgaacgg ggttggtacg tctacaacac
     2401 cctttcccag gcggccaaaa cttcccaggc acgcgtggcg gcttatctgg agaattccag
     2461 ggtggaatat caatcctact ggattaaaaa caccatcctg gtcaagagct ccaatctgac
     2521 caccctcaac ggcctgctcg gtttctccga aatcagcgcc attacccccc gacaaacttt
     2581 tatcctctat gaacccgaca ccagcgccgc ggttattgat aacggcgtga aagccgttga
     2641 acccaacctg acacatgtaa atgcggatga cgtctgggcg atgggtatcg acggcacagg
     2701 gctcgtggtg gccaatgtcg acactggcgt gcgttttaca caccaggcac ttgtcaatca
     2761 gtaccgcggc aatcttggcg gcggcagttt tgaccacaac tataactggt tcaaccccga
     2821 tgacctgacg gataacgctc cgcgcgatgg taacggtcat ggcacgcaca ccatgggcac
     2881 catggtgggc gatgacggcg gctcaaacca aatcggtatt gcgcccggag ccaagtggat
     2941 ggcctgcgcg ggctgcccgg acggcagctg tacggattct gctttgctgg gctgtgggca
     3001 gttcatcgcc gccccaaccg acttgacagg cggtaacgcg gacccggaca tgcgtccgaa
     3061 cgctgtgaac aactcctggg gcgactgcag tcagacctat gactcctggt acgcaagccc
     3121 catcgctgcc tggcaggctg cgggtgttta tccgatattt tccaatggca acgccagcaa
     3181 ctgcgggtat tcagcccctc ccggattgaa tacagttggt aatcctgccc gctccggcaa
     3241 cgttaccggc gtcggttcgt ccggcgaaca aaacggacag tacgccaccc actccaactg
     3301 gggtccgacc gataacgcgg acaccgtcaa cccgaccggt ggcttcgtct acatgaagcc
     3361 ccaggtgctc gcgcccggag tcagcatccg ctcctccaca cccggcagcg ataccgaata
     3421 ccaggacggt tggtcaggca cgtccatgtc ggctccgcac gtgaccggtc tggtcgcgct
     3481 gatctggcag gcagcgccgt gcctggttgg tgattatgcc accactgaaa atatcatcga
     3541 agaaacagcg gtgaatatca cctatgcgga cggttctacc gacaccccga cggattggcc
     3601 gaactacgcg acgggctggg gcgagattga cgccctggcg gcagtcacgc aagcctccgg
     3661 attgtgtgcg atgggaacgc tcgaaggcgt ggtgaccgaa gatggtacga ccccgatcga
     3721 aggcgcgcaa attctcgccg acaacggttc gggttatacc aagaaaatct acagcgcggc
     3781 agacggcagc tacagctccg gactgccgga aggcacctac accttaaccg ccagcaagta
     3841 tggttatgac acggtcagtg tgtccgatgt ggatgttacg gaagatgtga ccaccacgca
     3901 ggattttgtt ttggagcctc tgggcaccag tctggtttct ggcgttgtat atgacgcggg
     3961 cgtggagggg ctcggcgcac acggttatcc gctgtacgcg gcgattcatg tcacaaccgg
     4021 cagctttgac caaacgattt acagcgatcc gttcaccggt tactatgaaa ttgagctggt
     4081 ctcggatacg gaacacagct tcacgactac gccgctggct ccgggatatg aagaaaaagt
     4141 tgaggctgtt accccaagcg gcgcaaatta cacgcacgac attccgctcg tcgtggaagg
     4201 cgaggcctgc gcggctcccg gatacaagcc ggattacgac ttcttctact cctttgagag
     4261 cagcgacgaa ggtttcaccc ccggcgggac gacttccttt gcctggggcg actttaccag
     4321 cggtcctggc gagggacaca gcggcaccaa aggtatcgcc accaacccgg gcggcaatta
     4381 caaccccagc gaacttggct ggatggcttc cccggtattg gatatgactg gctatggcac
     4441 taactccatc gccatccaat ggtgggattg gaaagacatt gagagcgcga gctacgactg
     4501 ggcgcgtctg gatgtaacca aggacggcgg cgcgacctgg gatgtcgtct gggggcctgt
     4561 tggcggcgtt tctgatacgt cgtacagcca gcaaacggtg ttgctcgacc caagctacaa
     4621 cgtcgcgaac ttccagttcc gcttctactt caagagcgat agctcggtgc agtacgaagg
     4681 ctggtatgtg gatgacgtcg gcgttgtagc gattcccgtg cctccgccca caactgtctt
     4741 ctcctcgaat tttgactcgg ataacggcgg atttacggtt agcggcacga cgacctgggc
     4801 atggggagct ccgacttccg gacctggtgc ggcgcattcc gccccgaacg tgtgggcaac
     4861 caacctggca ggcaactata actccagcga atccgggtgg attacatccc ctgtgattga
     4921 cctgagcgct tatgccgggc ttgccccgac cctttccttc tggaactgga tggacaccga
     4981 aagtacgagc tttgactggg gcgttctgga agtcaccaag gacggcggcg ttagctggca
     5041 ggatgtctcc gggaagattg gggatcagct ctcctgggct aaaaaggtct atcaattaga
     5101 ttcctcttac gcagtcagca atttccagtt ccgcttctac ttccgcagcg acagctctgt
     5161 gaattatgcc ggctggtaca tcgacgatgt ggtggtgacc gtcgcggagc ctgtgtctgt
     5221 ttccgcgccc tgcacggtca ttcccggcgg tgcggtggct ggctttgtat ataatgacaa
     5281 cgacggcagc ccgctggtag gcgcggacgt gtacagcgct gaagtggcga cccaaaccat
     5341 ttccatccct gaggaccctg ccaacgaagg cttgtactgg gcattccagc cctttaccga
     5401 agagggcgcg cagggcacgc aatctctgac aggctcgaat gtggtctttg acccggcggc
     5461 cggcggggat gccggataca cacccggcgt tccggcaacg ctgtgcttta cagcgcattc
     5521 ttactcaccg gattgggaat atgtctatga cctttggacg cgcttcccga cggattggac
     5581 ggtaaccgat gtttacgtcc agggcacgcc gtattgtgcg tatggtgcat tcagcgactt
     5641 cagttggagt tttgaaacct caccctatga agtggatatt gaacatcaac gaagtcagtt
     5701 taattaccct gacgagaact gcgtcgcgac ctactgtatg gaagtcacgc caggctcttc
     5761 agccggaaat gtctcgtggt actgggacgg tgacaattgg gggaatgcgc cccaccatcc
     5821 ttgcagcagc gatgtttaca ctccggcttc aatggtggcc gtgccgtgcg atgagtgggt
     5881 aaatccgcag gcatccgtgc cgctgctggg ctcgttcgaa gcgcacgact tcacggcgga
     5941 gatggcgctc tttggcagcc agaccgaaac cgtgaacgtc atggaagact ttatcacccg
     6001 gcaggacttc tccctcggca ccggcgagct ggagtttgac cctgagtcct tcgaagttac
     6061 gatgatgatg ggcgacccgg ttcacgaaga aacgctgacc atcgggaaca acggctcctc
     6121 tcccgcgatg ttcgagctgg tggagaaaga ttccgggttc gtcccgccgc tttccatccc
     6181 agcctttacg gaagtgctgc ccgaggatac ccgcacgatt tcgatgggca aagcgcctga
     6241 agccgccaaa actgtcgggc tcgaacaagc gccaggcagc ctgacggata ttctggccgg
     6301 cgctcccgcg ttcgcgattg atatttaccc tggctacaac ctggtcaata tccccgacac
     6361 ggatgtgcct ggcgtctgga atatcgtcgg aagcatggga agccatgact tcttcgccgg
     6421 cgattttgtt ggcggagatt tcacgacgct ctatgctgtc gactacaaca ccaacggact
     6481 gtacgcaatc aatacggcca ctggtgcgca aaccttgata ggcccgaccg ccccgcctgc
     6541 cggccaaacc ttctccggcc ttaccggcac gcctgacggt acgatgtacg gcctggtaac
     6601 cagctgctcg gcatccagcc tggtcaccgt ggatatcgtc accggcgcca ccacgctttt
     6661 gggcgccctg cccggcattg gctgcggcat cgacctggcg tacaacacgg ctgatgacat
     6721 gatttacatc gtggaattgc tcacatccaa cctgatgaaa gtcgatcccg ccacgctggc
     6781 ggtgaccacc gtaggctcgc ttggggttgc ccctaattat gcccagggca tggactttga
     6841 agaagagtcc ggcgtgcttt actgggcggc ttacataagc tctggcgaac tgcgcgtgat
     6901 cgacacaacc acgggcgcta gcactctggt cggagccttc ccctccggcg cggaaactga
     6961 ctgcctggcc ttcccgaccg gcggcgtctc cgacgtgccg tggctttccg aagacccggt
     7021 aagcggtacg gttcccgctg gcggcgagca ggaggtcacc atcagcttcg acccgaccgg
     7081 cgccggcctg agccaacccg gcgattacct ggctgagctt aaagtcaagc acgacacacc
     7141 ctacacctat cctaatattc cggtgatctt gcatctgttt gcccccgata ctttcggcac
     7201 tgtcaatggt actgtcagcg gcctggaagc ctgcgatgtg aacccggaag ctttggaagg
     7261 cgcgacggtg aacttctggc agggcggcgc gattgtgtac accaccagca cgaacgccag
     7321 cggttactac acgtacaccg tgccagcggg aacctatgat atcgagtttg tggctgacgg
     7381 ctacgtgagc atcatggaat cggatgtcgc ggttgtgggc ggcagcacgc ttacgattga
     7441 taaggctctg cgcctgttgg cgccttgcct gagcgtcctg ccgaccgaac tcgaacagac
     7501 acaactggcg gacgtggtca ccacccagac gctcaccatt gtgaactccg gcgccgctgc
     7561 gggtattttt gaattgcaag aaatccctgt aattcaacag gaagctcttg gcccaacgta
     7621 cacttaccga cccgaactcg atggacctgc tctcgaccgg tccaagagcg gtcctaaaaa
     7681 cagcgcgaac gctccgacct atcaacccaa agacattctg ctggatgaag gcttcgaaag
     7741 cggcttcccg cctgctgatt gggccgtgca aagtcagtcg ccttggacct gggagttaag
     7801 cgattatagc ccgcattcgg gcctctataa cgcccacgtt ctgtatgatc aagatttgaa
     7861 cgagcaggat gaatggctcg tgacccctga attcaacctc aacgagggaa cgcttagctt
     7921 ctggtccatg ggcagcattt attggtgccg ggatacctac gacaactgtg atttgaacat
     7981 ctggttggtt gttgatgata ttggcggtgg agatgacatc ttcatccgag cagccgacag
     8041 cgactggacc gcaagctgga cctgggcgaa cacgactatt gaccttggtt cttacattcc
     8101 tggcggaccg gtgcgtatcg gcttccaata tgctggtctg gacggcgctg aaatcggatt
     8161 ggatgacgtc accatcgatg gaacagaagg tggggacgtg ccgtggctgt ctgaagaccc
     8221 gattgctggc acagtgccgg cagatggatc tcaggttatc accatcacct acgactccac
     8281 cggtctggcg gaaggcgatt actttgccac cttgcgtgtc aagaatccac cagctgtggc
     8341 cattaacatt ccggtgacgc tgcatatcac cagtgttctc caactctaca tcccgctgct
     8401 cttcaagtaa gccgcgcgtg taggattatt gtccaaagta gtttagcaaa atcatccggc
     8461 agagctttct gccggatgat ttctttcatg aacttcatga taaaatcaag ctccaatggg
     8521 gctgatgatg aaacaccttc ctctctatgg ttcaatcctg atcatcgcgc tgcttttcgt
     8581 gagctgctcc gcgccggccg cgccccaggc cagcccgacc gccgcgcctg acgcgcaaaa
     8641 ttgcctgccg gatgtgtggg tagcgccgga agtccccaag cgcatccggg ataactttgt
     8701 gctgccggcc agcctgcagt gggcgcaaaa ccgggggccg tgcagcgtca cgctcggcct
     8761 ggagagcctc agagcgcccg acgaggtcgt cttcgcgcaa atcctgtggg agtatgccct
     8821 ggtcgcgcct tcctggtcct tccgcgaggg ggcgggcagc gcggacttga aggcagcctg
     8881 ggggggcaaa gcgcgtggaa gcatgatgca aatcaagcag attatcgtgt acgcggaaga
     8941 ttacaacagc ttactcggtc tgttgggcga gcccgctgaa gacgggagtt tgagtgagat
     9001 tatcagcctg gctcctaacg aaaaaatcag cctctcagat cagcttggag cctcggtttg
     9061 gggaattgtg cccttagacc ggattcaagc cgattatcgc gtgctggcga ttgatgaagt
     9121 atcgccttta caagcggatt ttgaccgaca ggaatacgct ctgcggggca tttacagcct
     9181 gcatgccagc cccgaaacag ccgcccgcct gggggatgcg ggtatcgccg cgctgacgcg
     9241 cgggttggca ggcgaagagg cagacgcgca aaactctcag actcaagccg ccacggaaac
     9301 gcccgcccag ccggaaagtc ggcagactcc gaccgtgagc gttggtgaac gcgtccggca
     9361 gaaggatggg gcggtggaag cgctcatccc agccggtgtg ttcacttccg gctgcatcag
     9421 agagcataac ggcggctatg actgcccgga tgacgagctg cccagccgca gggttaatct
     9481 ggacgcgttt tatatcgata agtacgaggt taccaaccgc cagtacgcgc tgtgcgcggc
     9541 tgcgggggca tgccccgagc cggtttaccg ctattccgcc agccgggttt cgtactacgg
     9601 caatccggcg tatgacgatt acccggctgc caatatatcc tggacggaag ccgcagctta
     9661 ctgcgcctgg gccggcggca gactgcccac cgaagccgag tgggagaagg ctgcccgcgg
     9721 cacagatttg cgcgctttcc cgtggggtga cagcaatccc agctgcgcga aagccaacgc
     9781 gaaagacgaa cttttgggct caaactgcgt gggggatact ttgccggtcg ggagcctggc
     9841 ggagggggcc tcgccgtatg gcgtgatgga catggcgggg aatgtgtggg agtgggtgca
     9901 ggattggtac gtgctggacg agaacgggct gcgcatcgtg gtggatcctt tcgctcagga
     9961 tttaggcttc cacaaggtcg tcaagggcgg gtcctgggat tattcctgga gccggctgcg
    10021 gattgcttac aacagcgacc acgaggcgga cgcgcataaa atcagttttg gtttccgctg
    10081 cgccgcgccg gtggattgag acgcagggga ttgggcggtt atttgggcgg gattgcgtcc
    10141 gcggctccct gaatttcgaa tcacattgga actgcagaga ttagcctgag tttctatcaa
    10201 gcttaaactg gcggcgcgcc tctggagatt ttggcgcgcc gcgtttcatg ctggttaggt
    10261 ttaggaattc cgaaagatca atccgtctgg tcccacgtcc aggatgatgg tgtctccttc
    10321 gtggaattcg ccggaaagaa ttttgagggc gagtggatcc tgcacttccc gctggatggc
    10381 gcgtttgagc ggtcgggcgc caaattccgg ttcaaagcct atctctgcca gccaggccga
    10441 cgctgcggga gtgcttttga gagtgtaccc gcgcgctgcg agcagctccg ccacatgccg
    10501 gagttggatg ccgacaatct tttccaggtc ctctttttca agcggcttga agatgacaat
    10561 ttcatccacg cgattcagga actccggccg gaaaaagttt ttcaggattt cggtgacctg
    10621 gtcgcgggtg acatgcctgg ctttttccgg gtctgaccac aactgagaac ccagattgct
    10681 ggtcatgata atcagggtgt tgcggaagtc cacggtgcgg ccctgtccgt cggtcatacg
    10741 gccgtcgtcc agcacctgta aaagcgcgtt gaatacctcc ggatgcgctt tttcgatttc
    10801 gtcgaacaaa acgacgctat agggccgctt gcgcactgct tcggtaagct gcccgccttc
    10861 ttcgtatcca acgtagccag gcggcgcgcc aaacaggcgg ctgatggtgt gcttttcctg
    10921 atactcgctc atgtccactc ggacgatggc gttttgatcg acgaaaagga actctgccaa
    10981 agcgcgggca agctcagttt tgcctacacc ggtgggtcca aggaaaatga aggaaccaat
    11041 cgggcggtta gggtcttgca tgccagagcg tgagcggcgc acggcgttgg aaacagcccg
    11101 tactgcttca tcctgcccaa ccacgcgttg atgtaaccgg tcctccatgt gcagcagctt
    11161 ctgcatttcg ccctctaaca ggttcgtcac ggggatgccc gtccacttcg ccacgacctc
    11221 cgcaatttct tcggaatcaa cctcttcttt gagcagggcg ccgttttgct gaatttcttt
    11281 cagttttgct tcagctgtct ggattttttc gttcaactgg tgcagggttt cgtaacgcag
    11341 acgcgccgct ttttccaggt tggcatcgcg ctcggcctgt tctatttcct gttcggtttc
    11401 ctccagctgc gccttaagtg tgcgcaaatt ggaaatcgcg tctttttcgg tgctccagcg
    11461 caaattcaga atgctggatt tttcgcgcag gtctgtcagg tcttcttcga tctttgccaa
    11521 tcgatccctg gaagcctggt ctttttcttt ttgcagcgcc tgacgttcaa tttccagctg
    11581 catgatctgc cggtccactt catccagcgc ctgcggctta gagtctatct ctgcgcgcag
    11641 gtgggccgcc gcttcgtcaa tcaggtcaat ggctttatcc ggtaattttc tatcggtaat
    11701 atagcggctg gagagtgtgg cagcggcaat gacggcgggg tcggtgatgc gaaccccatg
    11761 atgcacttca taacgggatt taagaccccg caggatgctg attgtgtctt ctacggaagg
    11821 ttccagcacc atcacaggtt gaaaacggcg ttccagggcg gcatcctttt caatgtattt
    11881 gcggtattca ctggttgtgg tagcgccaat caagtgcaat tcgccccggg cgagcatggg
    11941 cttgaggatg ttccccgcgt ccatcgcgcc ttcggcagcg ccggcgccaa tgacggtatg
    12001 cagttcgtcg atgaagagga tgatggcgcc ttcggcgtcg gtcacttcct taagggcagc
    12061 tttcagccgc tcttcaaact caccgcggta cttggcgccg gcgaccagcg cggccatgtc
    12121 cagctggacc acctgcttct tttttaagcc ctccggcacg tcgcgtttga caatccgttg
    12181 ggcaagccct tccacaatgg cggtcttccc tacgcctggg tcgccgatca gggcagggtt
    12241 gttcttggtg cggcgggaga gaatctcgat tgtccgccgg atttcctcgt cgcggccgat
    12301 gacggggtcg agttttccct gacgcgcaag cgcggtcagg tcgcggccga acttttccag
    12361 cgcctgaaat gtggcttccg ggttttggct ggtt
//
LOCUS       DAOQAD010000052        12341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_5749_length_12341_cov_6.395328, whole genome shotgun sequence.
ACCESSION   DAOQAD010000052 DAOQAD010000000
VERSION     DAOQAD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12341
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5749_length_12341_cov_6.395328"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..947
                     /locus_tag="PKL60_05490"
     CDS             186..947
                     /locus_tag="PKL60_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95624.1"
                     /translation="MDGIITLDLLALAIVILALLCLGLALLTQRQHPYRNLRKNEALL
                     DAEKLMHEAAEKGLRLTIGLGAGQQTSAPAPANLAGLPVLRLMSRRSVFNDQPAQALA
                     GDGALAALAQLIVRGKYQNAFATELFKPDFAVLAGASPYAYLAGLLPEVNYRANAGVF
                     LFGSQRPEIILAASLAEQKDLPLVAGTDDLSAQAALFATNARLCLGEDALHAGAALET
                     DPGAAASVRAQDWLRVLAVLGLLVGAALKLLGVWQ"
     gene            938..1558
                     /locus_tag="PKL60_05495"
     CDS             938..1558
                     /locus_tag="PKL60_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95625.1"
                     /translation="MAVKKTVPVLVAAAAALFILAALFLGEPLAGWLGIVLNWAVITA
                     SAALLVAIAALILTHLRNVLGGKRGFLFSLILISAFLASFIGALVLGVDNGEYLRWVA
                     AVQLPLESSLLGLAALVMTAAALQIFRNRGWSLLTISFGISALVFLVIALGLLQALNI
                     PLLTAVLNYLQRLPLIGARGLLIGIGLGALLMGLRVVLGLERPWGE"
     gene            1559..3394
                     /locus_tag="PKL60_05500"
     CDS             1559..3394
                     /locus_tag="PKL60_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNW95626.1"
                     /translation="MSAELILCPHCGKENLPQATRCVYCGEDLEELFRIEGVEDNTAL
                     QDEPGISTADLVEALRQADSQKDEKTGAADRNGQNEAADSGGETESEPPAGEDEQTPE
                     WLARIRKRAQQEEDAAGELTRKGSSLDTARSEDHQGRVDQEFASWIARIRENARRENL
                     LTPKPAADSAESEGGVPEWLRKVRELQPEAEEEEPLAEEPAPEEAELKEGQNDAAEQV
                     RAEEGEPNKETSPDENVWESASAPEEALETEPEPEPVREHEEAAEEAQTPAETPEAEE
                     SAPAPKTPELEPVLEELGLAAASAAVELEEKPASSAELGADLLLLRRQKENAQILMNL
                     IGEEGRGLPPAKTQRVSGRGWYRFILGIFLLLVLAAVLVFVPEAKPGQVENPAPAAAL
                     KEALGRLASGDKVLVVLEYQAADSAELEQITAPVLKALQTRGVEMQFLTTHPNGLWLA
                     DALMRKADIPAHETGFLAGGKLGVLAAALNPDGESALVPAGVEKLENYALILLLEDSQ
                     MDIQNWLEQAGPWLGADKIGVLASSQNAALLLPYYDAGQLAGFAAGTTEGRVLAQALD
                     RTDLPSAPSRAFQAGLLVMIAVILLGVLSKVEADSGARRPKEPEA"
     gene            3391..4074
                     /locus_tag="PKL60_05505"
     CDS             3391..4074
                     /locus_tag="PKL60_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95627.1"
                     /translation="MNAMDIVLGIIGIALSVMIFSYLLGDNFFFRVALYILVGVSSGY
                     AAAVLLTKVILPRLIEPLKQSGTDAFWLALVPLVLCLLLSLMLIPRLVKAGTLPLAFL
                     AGAGAAVAIAGIPRGTLAPQLLSVVNRFSPELLRENAGADWGGILEALFILLGVVSVL
                     FYFHHRLSRKSAGAQRPPLVEGISSVGQLFLGITLGMLFVGFYSAALTALIARMVWLK
                     EFILALLTG"
     gene            4088..5785
                     /locus_tag="PKL60_05510"
     CDS             4088..5785
                     /locus_tag="PKL60_05510"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNW95628.1"
                     /translation="MEKRRTLSVDIGSQNTRAWLFEQREEGYALVCSAVAETSAASGQ
                     DLRGGVNRALEALQEKSGLRLLDLQQKLIRAEEGVGACGLSLSAGKPIRTVLVGISDT
                     YSLAALRRLVSLFYTDVVLEIDLQSDLNATAQLEKLIAANADLFVCAGGTNQGAIRPV
                     RAALDNLRIVYQSLPRLIRPQLVYAGNEALTDYAREELEAGEDFHRARNIQARTDAES
                     LAAAWPAMLGAFRRLRRQQLPGLAELEQDWECEALPSAFAASRIVRLIDRMNPGGKGA
                     MALDVGCNSTIVLAARGEDFIGSISHPPISEQVGKETSRYSTLPIDAQTAAAYMLNKK
                     LHQAFLPATLEDLAIEQAWTRVRLHYALRRTRELFPWFGYDPELGLLDPYEPVLLSGE
                     SLVRVPAPQQTLLMALDGLQPHGITTFAMDKDQLLAGLGSLAEKEALLPVQVIDSGVF
                     SNLGTAICADASERSSRRVLTLEIDRGESARETLEIRKGEIKRVETTPPGPTRIYLSP
                     NELTDVGMGFPGLGGWVTVPPSEVGTVIDARGRPLELPEEMTRRSEAFYNWLWELGG"
     gene            5787..6842
                     /locus_tag="PKL60_05515"
     CDS             5787..6842
                     /locus_tag="PKL60_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95629.1"
                     /translation="MSTEILAIACDQQILRSYTLPFTGSVLVRTGQEVEAGQPVAEIQ
                     MPERYQVFDAINGLKINPKNIDRHIERLVGEPVKAGDVIAQKSGLISRLFRASQDGMV
                     VAIRDGRITLALGEKKLQVLATFPGTVVEIIPERGAVVAVQGLLLQGVWGNGLNSSGE
                     LTRLEPTESAAGKKQTTADLQGKIAALPHIADEAAFTALLAHKPAGLVLGSVMPELLP
                     ALAEAPLPVMVLVGYGEYEIDTLSTAMLRQMEGQMVSLNANEADASEQSRPDLILPGK
                     AQVSPGLFHEELQLEPGRHVRLLGKPYTGSIGEIVELPAEPEKFASGLVMRAVVVRRE
                     DELIVRVPRENVELILE"
     gene            complement(6846..8030)
                     /locus_tag="PKL60_05520"
     CDS             complement(6846..8030)
                     /locus_tag="PKL60_05520"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNW95630.1"
                     /translation="MNLPTSQTSPDGFNSLPPNQKRRLRLIIRSASAAEIDSFLHDDL
                     QPKTALNPLYFVMLALSALLLAAALAANSPLLLLAALLCAPFNSPLMGLTLSAVIPSW
                     GLMLRSFAALLFTAAVYFGAGWLAALLPGRLTEGDGLPFLHLLQHGWLEWTALVVGSV
                     LLAAMLIRQRETPRLASALITALLFFPLGLAGWALQAGFPYASAGLSVLSGAYAAVSL
                     LCALITFWAAGLPPRRGAGWVLFVLAIGLSLLALFGFLSTKHHLTQTIALNSTPPPGG
                     TPTQPGVALPPTQTPLPPTPTIPTPTSAVQNPVVTALPSITPTPTLVSALIRSDTGVI
                     VRSEPGSTASVVTYLNDLTELVLLGERVEKDGILWEKVRLENGKTGWVSARFVITATP
                     AK"
     gene            8190..9257
                     /locus_tag="PKL60_05525"
     CDS             8190..9257
                     /locus_tag="PKL60_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95631.1"
                     /translation="MNTRKISRALLLLLLGAALLAGCVRTTPPEPTVTATNTVIPAPT
                     LIIADTATPEPTPEPLPPITANVVLWAPANADFSQAQTLPSDLAAYAGANGLVFEQRG
                     QLSAEELGSAVRLVVALAPAAEISALAAAAPAVQFLAVGVPDVQTGANLHALSSSSAT
                     LEERAFLAGYLLGMVIPDYRVGVISQAGTEDGTKTNDSFAVGARYFCGLCNSRFGPIL
                     FYPKGAQISDPANPGDWQAAADLLLADSVKGVFIQPEVSSPELVNYLQAAGVQMVGVS
                     GQAGYNESPLWLGLLGSDLSADAVNLAARLLQGGQVGALKTGITLTHINADVITEGKQ
                     RLFDVTVQDVQSGFVRVTPVQ"
     gene            9466..9537
                     /locus_tag="PKL60_05530"
     tRNA            9466..9537
                     /locus_tag="PKL60_05530"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9498..9500,aa:Thr,seq:tgt)
     gene            9621..9703
                     /locus_tag="PKL60_05535"
     tRNA            9621..9703
                     /locus_tag="PKL60_05535"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9655..9657,aa:Tyr,seq:gta)
     gene            9740..9812
                     /locus_tag="PKL60_05540"
     tRNA            9740..9812
                     /locus_tag="PKL60_05540"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9773..9775,aa:Thr,seq:ggt)
     gene            9859..11064
                     /gene="tuf"
                     /locus_tag="PKL60_05545"
     CDS             9859..11064
                     /gene="tuf"
                     /locus_tag="PKL60_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNW95632.1"
                     /translation="MAKQKFDRSKPHMNVGTMGHIDHGKTTLTAAITKVSALRGFGDF
                     RAFDSIDNAPEEKSRGITINISHVEYSTPKRHYAHVDMPGHRDYIKNMITGAAQVDGA
                     ILVVSAPDGPMPQTYEHVLLAHQVEVPAIIVFLNKVDMMDDPELLELVEMEVRELLTK
                     NHFPGDEVPIIHGSAKLALESTSTDPNAPEYKPIIELLDAVDNYFQDPVRELDKPFMM
                     PVEDVFSIKGRGTVVTGRIERGQVKLNDPVEIVGLREKSMNSVVTGVEMFHKQLDTGM
                     AGDNAGLLLRGIDRDDVERGMVIAKPGSITPHTTFECSAYILKREEGGRHSAFFTGYR
                     PQFYIRTMDVTGDVTLPEGVEMVLPGDNVNGMKVKLITKVALEQGSEFAIREGGLTVG
                     AGKITKIIE"
     gene            11142..11291
                     /gene="rpmG"
                     /locus_tag="PKL60_05550"
     CDS             11142..11291
                     /gene="rpmG"
                     /locus_tag="PKL60_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNW95633.1"
                     /translation="MRPVINLACTECKERNYTSVKNRRNDPGRLELKKYCPRCRMHRV
                     HREVK"
     gene            11326..11398
                     /locus_tag="PKL60_05555"
     tRNA            11326..11398
                     /locus_tag="PKL60_05555"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11359..11361,aa:Trp,seq:cca)
     gene            11459..11674
                     /gene="secE"
                     /locus_tag="PKL60_05560"
     CDS             11459..11674
                     /gene="secE"
                     /locus_tag="PKL60_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNW95634.1"
                     /translation="MAQIETKPNFFQKIKRFWRETVGELRKVTWPTPQEAWALTKLVL
                     VVTLIMAALLGFMDFVFSQLIKLLVAG"
     gene            11723..>12341
                     /gene="nusG"
                     /locus_tag="PKL60_05565"
     CDS             11723..>12341
                     /gene="nusG"
                     /locus_tag="PKL60_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNW95635.1"
                     /translation="MEDKKPIEGLPAEEQTATPDEGASARPAEGASGAGKPAASGQDD
                     ARQWFVVHCYSGYENKVRKNLEQRIETMNMKDKIFDVVIPTQEEIEVRDGKRRTVERH
                     VFPGYVLVNMILEEESWFVVRNTPGVTGFVGMGDEPTPLRPEEVAQILRRMEDESPTY
                     KVTYHAGDRVRIVDGPFNDFRGTVAEVNMERSKIRVMVNFFGRETP"
BASE COUNT         2544 a         3524 c         3879 g         2394 t
ORIGIN      
        1 attcttcact caaggtgaat atgctatact ttatctatgg gagttgggct gtttgatgca
       61 cacctgaaag ttcaacaacc ggatacgcgg tctctgccgg cttgctcaat acttgcttcc
      121 ggccagctca aaaccgcggg aatttttccc cttttgggac taacaatgct caggggcctt
      181 tcagcatgga cgggataata acgcttgacc tgctcgcgct cgcaatcgtc attctggcgc
      241 tgctttgcct cgggttagcc ctccttaccc aacgccagca cccctaccgc aatttacgca
      301 aaaacgaagc gctgctggac gccgaaaaac tgatgcacga agccgccgag aaaggcctgc
      361 gcctcacaat tggcctgggc gccggccagc agacaagcgc gcccgcgcct gccaatctgg
      421 ccgggctgcc cgtgctgcgc ctgatgtcca ggcgttcggt ctttaacgac caacccgcgc
      481 aggcgctggc gggtgacggc gcgttagccg cgctcgccca gctcatcgtg cgcggaaaat
      541 atcagaacgc gttcgcaacg gaactcttca agcccgattt tgccgtgctg gccggcgcaa
      601 gcccctatgc ctacctggcc ggcctgctgc ccgaggtcaa ttaccgcgcc aacgcgggcg
      661 ttttcctgtt tggcagccag cgacctgaaa ttattctggc cgccagcctg gcggagcaaa
      721 aagacttgcc gctggtggct ggcacggacg acctctcagc gcaggcggcg ctgtttgcca
      781 ccaacgcgcg cctctgcctg ggggaggacg cgctgcacgc tggcgcggcg ctggaaactg
      841 accccggcgc ggctgcttcc gttcgcgcgc aggattggct gcgggtattg gccgtgctcg
      901 gattgctggt gggagccgcc ctgaaactgc tgggggtatg gcagtgaaaa agacggttcc
      961 agtcctcgtt gccgccgccg cggccttgtt catccttgcg gcgcttttcc tgggtgagcc
     1021 tttggccggc tggctgggga ttgtcctgaa ttgggcggtc atcactgctt ccgccgcgct
     1081 gctggtcgcc attgctgctc tcattctgac tcatttacgc aatgttctcg gcggaaaacg
     1141 cggctttctt ttcagcctca tcctcatcag cgccttcctg gccagcttta tcggcgcgct
     1201 ggtgctgggt gtggataacg gcgaatattt acgctgggtg gcggccgtcc agctgccatt
     1261 ggaatcaagc ttgctcgggt tggcggcgct ggtgatgaca gccgccgcgc tgcaaatctt
     1321 ccgcaaccgc ggctggtccc tgctgacgat ttcctttggc atcagcgcgc ttgtttttct
     1381 cgtgattgct ctcgggctgc tgcaggcgct gaatatcccc ttgctgaccg cggttcttaa
     1441 ttatctgcag cgcctgcccc tcatcggagc gcgcggtttg ctgattggca tcggcttggg
     1501 cgcgctgctg atgggtttgc gcgtggtgct tgggctggaa aggccctggg gtgaatagat
     1561 gagcgcggag ctgatcctct gtccgcactg cgggaaagaa aacctgcccc aggccacgcg
     1621 ctgcgtatac tgcggtgaag acctggaaga gctgttccga atcgaaggcg tggaagataa
     1681 taccgcgctg caggatgaac cgggtatcag cacggctgac ctggtagaag ctctcaggca
     1741 ggcagattcc caaaaggacg aaaaaaccgg agcggcagac cggaacggtc aaaacgaagc
     1801 tgcggacagc ggaggcgaaa ccgaatcaga accccctgcc ggcgaggatg aacaaacccc
     1861 ggaatggctg gcgcgcatcc gcaaacgcgc ccagcaggaa gaagacgcgg ccggcgagct
     1921 tacccgcaaa ggcagctctt tggataccgc ccgcagtgaa gaccatcagg gacgcgtgga
     1981 tcaggaattc gccagctgga tcgcgcgcat ccgcgagaat gcccgccggg aaaacctgct
     2041 gacgccgaaa cccgcggcag acagcgctga aagtgaaggc ggcgtgccgg aatggctgcg
     2101 caaagtgcgc gaactgcagc cggaagcgga agaggaagag ccgctcgcgg aagagcctgc
     2161 tccggaagaa gccgaactga aagaagggca aaacgacgcg gcggaacagg tgagggctga
     2221 agaaggcgag ccaaataaag aaaccagccc ggacgagaac gtttgggaaa gcgcgtccgc
     2281 gcctgaggaa gctcttgaga ctgagccaga acctgaacca gttcgggagc atgaggaagc
     2341 cgctgaggaa gcgcaaacac ccgcggaaac ccctgaagcg gaagaaagcg cgccagcccc
     2401 caaaacgccg gagcttgagc cggtgctgga agaattgggc ttggctgccg caagcgcggc
     2461 ggtggaactg gaagaaaagc cggcctcgag cgcagagctt ggcgcggatt tgctgctgct
     2521 gcgccggcaa aaagaaaacg cgcaaattct gatgaacctg attggcgaag aaggccgcgg
     2581 gctgcctccg gccaaaaccc agcgggtcag cggcaggggt tggtaccgct ttatcctggg
     2641 catatttctg ctgctggtgc tggcggctgt gctggtattt gtgcccgaag caaaacccgg
     2701 acaagtcgaa aacccggctc cggctgcggc cctgaaagaa gcgctgggca ggctggcaag
     2761 cggggataaa gtgctggtcg tgctggaata tcaggcggct gacagcgcgg aattggaaca
     2821 gataaccgcg ccggtgctga aggctttgca aacgcgcggg gtggaaatgc aattcctgac
     2881 cacgcacccc aacggtttgt ggctggcaga cgcgctgatg cgcaaggcgg atatccccgc
     2941 gcatgagaca ggttttttgg ctggcggaaa gctgggtgtg ctggcagccg ccttaaaccc
     3001 ggacggtgag agcgctctgg tgcccgcggg cgtagaaaaa ctggagaatt acgcgctcat
     3061 cctgcttttg gaagattcgc agatggatat ccagaattgg cttgagcagg ccgggccctg
     3121 gctgggcgct gacaaaatcg gcgtgttggc cagcagccag aatgccgctt tgctgcttcc
     3181 ttattatgac gccggccaac tggcaggctt cgccgcgggc acaacggaag gccgcgttct
     3241 ggcgcaggcc ttggaccgga cggacctgcc gagcgcgccc tcgcgcgcgt tccaggcggg
     3301 cttgttggtg atgattgccg tgattttgct gggcgtcctt tccaaagtcg aggcggactc
     3361 gggcgcgcgc cgcccgaagg agccggaagc atgaacgcga tggacattgt gctgggcatc
     3421 attggcatcg cgctttccgt gatgattttc agctacctgc tgggcgataa tttcttcttc
     3481 cgcgtcgcgc tgtatatcct ggttggggtt tcctccggtt acgcggctgc cgtgctgctc
     3541 acgaaagtta tcctcccgcg gctgattgaa ccgctgaagc aaagcggcac agacgcgttc
     3601 tggctggcgc tggtgccgct ggtgctgtgc ctgctgctct cgctgatgct gataccccgc
     3661 ctggtcaaag ccggcacgct tcccctggcg tttttggctg gcgctggcgc tgccgtcgcg
     3721 attgccggca tacctcgcgg aacattagcg ccgcagctgc tctccgtcgt gaaccgcttc
     3781 agccccgaat tgctgcgcga gaatgccggc gcggattggg gcggaattct ggaagcgctg
     3841 ttcattctgc tgggcgtcgt ttccgtgctg ttctacttcc accaccgcct gagccgaaaa
     3901 agcgccggcg ctcaaaggcc gccgctggtg gaaggcattt ccagcgtggg gcaactcttc
     3961 ctgggaatca cccttggcat gctctttgtc ggcttttaca gcgcggcgct gaccgcatta
     4021 attgccagga tggtctggct gaaagagttc atcctggctc tcctgaccgg gtagagggat
     4081 gctggctatg gaaaagcgca gaaccctgag tgtggatatt ggctcgcaga atacgcgtgc
     4141 gtggcttttc gagcagcgcg aagagggcta cgcgctcgtt tgcagcgctg ttgccgaaac
     4201 tagcgcagct tccgggcagg acttgcgcgg gggcgtgaat cgcgcgctcg aagcgctgca
     4261 ggaaaaatcc ggtctgcgcc tgctcgacct gcagcaaaag ctaatccgcg ctgaggaagg
     4321 cgtcggcgcc tgcgggctca gcctgtcggc aggcaaaccc atccgcacgg tgctggtagg
     4381 catatcggac acctactccc tggcagcgct gcgccggctt gtcagcctgt tctacacgga
     4441 cgtggtcttg gaaattgacc tgcagtcgga cttgaacgcc accgcccagc ttgaaaagct
     4501 catcgccgcg aatgccgacc tgtttgtatg cgccggaggc accaaccagg gcgcaatccg
     4561 gccggtgcgg gcggcgctgg acaatttgcg cattgtctac caaagcctgc cccgccttat
     4621 ccggccgcag ttggtctacg ctggcaacga agctctgaca gattatgcgc gcgaagaact
     4681 ggaggccggt gaggacttcc accgcgcgcg aaacatccag gctcgcacgg acgcagaatc
     4741 gctcgcggca gcctggccgg caatgttggg cgcgttccgc cgcctgcgcc ggcagcagct
     4801 gcctgggctg gccgaactgg agcaggactg ggaatgcgaa gcgctgccca gcgcttttgc
     4861 tgccagccgc atcgtgcgct tgattgaccg catgaacccc gggggcaaag gcgccatggc
     4921 gctggacgtg ggctgtaatt ccaccatcgt gctcgcggcg cgcggagaag actttatcgg
     4981 ctccatcagc catccgccca tctcggaaca ggttggcaaa gagaccagcc gctattccac
     5041 gctgcccatc gacgcgcaaa cggccgcagc ctacatgctc aacaagaagc tgcaccaggc
     5101 attcctgccc gcgacgctgg aagacctggc gattgagcag gcctggacgc gcgtgcgttt
     5161 gcattacgcc ttgcgccgca cgcgggagct gtttccctgg tttggatacg accctgagct
     5221 gggtttgctg gacccttacg aaccggtcct gctttccggt gagagcctgg tgcgcgtacc
     5281 tgcccctcag cagacgctgc tgatggcgct ggacggcttg cagccgcacg ggattaccac
     5341 tttcgcaatg gataaagacc aattactagc gggtttgggc agcctggcgg agaaagaagc
     5401 gctgctgcct gtgcaggtca tcgattcggg tgttttttcc aatctgggca cggctatctg
     5461 cgcggacgcc tcagagcgct cctccaggcg ggttttaacc ctggaaattg accgcggtga
     5521 gagcgctcgc gagacgctgg aaatccgcaa gggcgaaatc aagcgcgtgg aaacaacgcc
     5581 ccccggcccg acgcgcattt acctttcccc gaatgagctt acggatgtag gcatgggttt
     5641 tcccggcttg ggcggctggg tgacggtgcc gccttccgag gtgggcacgg taattgacgc
     5701 gcgcggaagg ccgctggagc tgccggagga gatgaccagg cgtagcgagg ctttctacaa
     5761 ttggctgtgg gagttaggcg gctagcatga gcactgaaat ccttgcgatt gcctgcgacc
     5821 aacagattct gcgcagctac accctgcctt ttacggggag cgtgttggtg cgcaccggcc
     5881 aggaagtaga agccggccag cctgtagcgg aaattcagat gccggagcgc taccaggtct
     5941 ttgatgccat caacgggttg aagattaatc cgaagaatat cgaccgccac atcgaacggc
     6001 ttgtgggcga accggtgaag gccggcgatg tgattgccca gaagtccggg ctgatttccc
     6061 gccttttccg cgcttcgcaa gacggcatgg ttgtggccat ccgggacggc aggattaccc
     6121 tggccctggg cgaaaagaag ctgcaagtgc tggcaacttt cccgggaaca gtggtcgaaa
     6181 tcattccgga acgcggcgcg gtggtggctg tgcaaggctt gctgctgcag ggcgtgtggg
     6241 gcaacgggct gaacagctcc ggagagctga cgcgcctgga acccacggag tccgccgccg
     6301 gtaaaaaaca gaccactgcc gacctgcaag gcaaaatcgc ggcgctgccg catatcgcgg
     6361 atgaagcggc ttttaccgcc ctgcttgctc acaagccggc gggcctggtt ttgggctcag
     6421 tgatgcccga gctgctgccc gccctcgcag aagcgccgct gccagtgatg gtgctggtcg
     6481 ggtacgggga gtatgaaatt gacacgctca gcacagccat gctgcggcag atggaagggc
     6541 agatggtttc cctgaacgcg aatgaggcag atgcctccga gcaaagccga ccggacctga
     6601 ttttacccgg caaggcgcag gtctctccgg gtttattcca cgaggagctg cagctggaac
     6661 cgggcaggca cgtgcggctg ctgggcaagc cctataccgg cagcatcggc gagattgtgg
     6721 aactccccgc agaacccgaa aagtttgcca gcgggctggt gatgcgcgcg gtcgttgtgc
     6781 ggcgcgagga cgagctgatt gtgcgcgtcc cgcgtgaaaa tgtggaactg attttggaat
     6841 aggccttatt tggctggcgt ggctgtgatt acaaagcgcg ccgaaaccca cccggttttt
     6901 ccgttctcca gacgcacttt ttcccagagg atcccgtcct tttctactcg ttcccccaga
     6961 agcaccagtt ctgtcaggtc gttcagataa gtaacgacgc tggctgtgct gccgggttcg
     7021 gagcgcacga ttacgcctgt gtccgagcgg atcagggcgc tgacgagtgt cggggtcggg
     7081 gtaatgctgg gcagagctgt caccaccggg ttttggaccg cggaggtggg ggtaggtatt
     7141 gtgggggtag gcggcaaagg cgtttgggtg ggcggcagcg ctacgccggg ttgggtgggg
     7201 gtgccgcctg gcggcggggt gctgttgagg gcaatggttt gcgtcaggtg gtgcttggtg
     7261 gaaaggaagc caaacaaagc cagcaggcta agtccaatcg cgaggacgaa aaggacccaa
     7321 cctgccccgc ggcgcggtgg aagaccggca gcccagaatg taatcagggc gcacaagagg
     7381 gagactgccg cgtaagcgcc ggagagaacc gacaacccgg cgctggcgta tggaaaaccc
     7441 gcctgcagcg cccaacccgc cagccccagc gggaaaaaca acaaggctgt gatgagagcg
     7501 gaagccaggc gcggcgtttc gcgctggcgg atgagcatgg cggccagcag aacgctgccg
     7561 accacgagcg cggtccattc cagccagccg tgctgcagca gatgcaggaa gggcagtcca
     7621 tccccttccg ttaaccggcc aggcagcagc gcggcgagcc agccggcgcc aaaatacact
     7681 gcggccgtga acagcagcgc ggcgaaagag cgcagcatca gcccccacga agggatgacc
     7741 gcgctgagcg tcaggcccat cagcgggctg ttgaagggcg cgcacagcag tgcggccagc
     7801 aaaagcaagg gggaattggc agcaagcgcg gcagccagaa gcagcgcgct gagcgccagc
     7861 atcacaaagt acagcggatt caaagcggtt ttaggctgaa ggtcgtcgtg taagaagctg
     7921 tcgatttcgg ctgcgctcgc gctgcggatt atcaggcgca agcggcgttt ttggttggga
     7981 ggcaggctgt taaacccgtc cggactggtt tggctggttg gcaaattcat gggaatccgg
     8041 tctcctcgct tgagatgaag ctgcttgtgc cgctgatact gcctgacaaa gattataccg
     8101 cctttgagcg tgctttcctt tctgatgcaa aattcatttc gtaaacactt ggatgtgaca
     8161 aacgcccggc atgcggtaaa attggcggca tgaacacccg caaaatatcc agagcattgc
     8221 tgctcctgct gcttggcgcg gcgctgttgg cgggctgcgt gcgaacgacg ccgcccgaac
     8281 caaccgtgac agctaccaat accgtaatcc ctgccccgac gctgattatc gcggatactg
     8341 ccacgccgga acctacgccc gagcccctgc cgccaatcac ggcgaatgtg gtgctctggg
     8401 cgccggccaa tgccgatttc tcgcaggcgc agacgctgcc atctgacctg gcagcctacg
     8461 cgggcgccaa cggattggtc ttcgaacagc gcgggcagct cagcgcagaa gagctgggaa
     8521 gcgctgtgcg cctggtggtc gcgcttgccc cggcagctga aatcagcgcg ttggctgcgg
     8581 ccgcgcccgc cgtgcagttc ctggcggtcg gcgtgccgga tgtgcagacc ggcgcaaacc
     8641 tgcacgcgct gagcagctcc tccgccacgc tggaagagcg cgcgtttttg gccggctacc
     8701 tgttgggcat ggttatcccg gattaccgcg tgggcgtcat cagccaggcg ggcacggaag
     8761 acggtacgaa aaccaacgat tcttttgcgg tgggcgcgcg ttatttttgc gggctgtgca
     8821 attcgcgctt tggacccatc ctgttctatc ccaagggcgc ccagataagc gaccccgcca
     8881 acccggggga ctggcaggcg gctgcggatt tgctcctggc ggattccgtc aaaggcgtat
     8941 tcatccaacc cgaagtaagc tcacctgaat tggtgaacta cctccaggcg gccggcgtac
     9001 agatggtcgg ggtgagcggg caggcaggct ataacgaaag cccgctgtgg ctgggcttgc
     9061 tcggctcgga tttgagcgcg gacgcggtga atctggccgc gcgcctgctg cagggcgggc
     9121 aggtgggcgc gctgaaaacc ggcatcacgc tcacgcatat caacgcagac gtaattacgg
     9181 aaggcaaaca gcgcttgttc gatgttaccg tgcaggatgt gcagtccggc tttgtgcggg
     9241 tgacgcctgt ccagtagaaa aattccggcg gttccacagc gccgcggcct aaaagctgta
     9301 gaaacagggc ctggtcggtt tgaatctgct tgacgaaagg gtttatctct gatagactca
     9361 cgctttgctg gaatggcagg cgcccgaatc ggtcttgcag tttaacaagg catttgatat
     9421 acttgttcag ctaaaagccg atatttaata atttaataga agggcgccgg cgtagctcaa
     9481 tggtagagca accgccttgt aagcggtagg ttatgggttc gattcccatc gtcggctcag
     9541 attaaccgtt agcgattgag attgatgctg atcaacctcg atctccagcg ggtaaagagc
     9601 taagcgcccg gcagcaataa ggacggttac ccaagtggac aacggggact gactgtaaat
     9661 cagttggcag aggccttcgg aggttcgaat ccttcaccgt ccatctcaga tcacaggaat
     9721 gtgacctgaa tttacggctg ccctcatagc tcagttggta gagcgcgttc ttggtaagaa
     9781 cgaggtcatg ggttcgaatc ccattgaggg ctttacgccg agatttgaaa acggaatgta
     9841 tttattaagg agataagcat ggcaaaacaa aaattcgacc ggtctaaacc ccacatgaac
     9901 gtgggcacca tgggccacat tgaccatggt aagaccacac tgacagccgc gattaccaaa
     9961 gtttccgccc tgcgcggttt tggtgatttc cgcgcgttcg attccatcga caacgccccg
    10021 gaagaaaagt cccgcggtat taccatcaac atctcgcatg ttgaatactc gacccccaag
    10081 cgtcactacg cccacgtcga tatgcccggc caccgcgact atatcaagaa catgattacc
    10141 ggcgctgccc aggttgacgg cgcgattttg gttgtttccg ctccggacgg cccgatgccc
    10201 caaacttacg aacacgtttt gctggcgcac caggtggaag tccccgctat catcgtcttc
    10261 ctgaacaagg ttgacatgat ggacgaccct gaactgctgg aactggttga gatggaagtc
    10321 cgtgaactgc tgaccaagaa ccacttccct ggcgacgaag tcccgattat tcacggctcc
    10381 gccaaactgg ctctggaaag cacttccacc gacccgaatg ccccggaata caaaccgatc
    10441 atcgagctgc tcgatgctgt cgacaactac ttccaggatc ctgtccgcga gttggacaag
    10501 cccttcatga tgcccgtgga agacgtcttc tcgattaaag gccgcggcac tgtggttacc
    10561 ggacgtatcg aacgcggtca ggtcaagctg aacgaccccg ttgaaatcgt tggtttgcgc
    10621 gagaagagca tgaactccgt ggtcaccggc gtggaaatgt tccacaagca gctggatacg
    10681 ggcatggctg gcgacaacgc tggtttgctc ctgcgcggta tcgaccgtga tgatgtggaa
    10741 cgcggcatgg tcattgccaa acccggcagc atcacccccc acaccacctt cgagtgctcc
    10801 gcatacattc tgaagcgcga agaaggcgga cgccacagtg cgttcttcac cggctaccgc
    10861 ccgcagttct acatccgcac catggatgtg actggcgacg tgaccctgcc tgaaggcgtt
    10921 gagatggttc tgcccggtga taacgtgaac ggcatgaaag tcaaactgat caccaaggtt
    10981 gctctggaac agggttccga attcgctatc cgcgaaggcg gcctgaccgt cggcgctggc
    11041 aagatcacga agattattga gtagaatatt ctgatggagg aaacatggct gcctccaaaa
    11101 ggcagccgtg ttaaatagat atggcgtcaa aaaagaaaga tatcagacca gtcatcaacc
    11161 tggcatgcac tgagtgcaaa gaacgcaatt acacttccgt caagaaccgc cgcaacgacc
    11221 ctgggcgtct tgaactgaag aagtactgcc cgcgctgccg catgcaccgc gtgcaccgtg
    11281 aggtgaaata gcgcgcgtct gtcgtaagac gggcagcgct tgcttaggtc agtagctcaa
    11341 ttggcagagc accgctctcc aaaagcgggg gttgtgggtt caattcctac ctgacctgtt
    11401 gagaaggttt caacgccgta caagttgcgg cgttgagttt tcaaccagga gagatgttat
    11461 ggcacaaatt gaaacgaaac caaatttctt tcagaagatt aaacgcttct ggcgtgagac
    11521 tgtcggcgag ctgcggaagg tgacctggcc taccccccag gaagcctggg cgctgaccaa
    11581 actcgtttta gttgtgaccc ttatcatggc tgccttgctg ggttttatgg attttgtgtt
    11641 cagtcagctc atcaaactgc tggtagcagg ataaaacgtt gcaggaaagc ccacgagggc
    11701 tgtggatatg gataacgcag aaatggaaga caagaagcca atcgaaggct tgcccgctga
    11761 agagcagacc gcgacccctg atgaaggggc ttctgcgcgt cccgctgagg gtgcttccgg
    11821 cgcgggaaaa cccgctgcaa gcggccagga tgacgcgcgc cagtggttcg tagtccactg
    11881 ctattccggc tatgagaata aggtgcgcaa aaacctggaa cagcgcatcg agaccatgaa
    11941 catgaaggac aaaatcttcg acgtggtcat ccccacgcag gaagagatcg aagtccgcga
    12001 cggcaagcgc cgcacggtgg agcgccacgt cttccccggg tacgtcctgg tgaatatgat
    12061 cctggaagaa gaatcctggt ttgtggtgcg caacactccc ggcgtcaccg gctttgtggg
    12121 catgggcgac gaacccaccc ccttgcgccc ggaagaggtc gcgcagattt tgcgccgcat
    12181 ggaagacgaa tcgcccacct ataaggtcac ttatcacgcc ggggaccggg tgcgcatcgt
    12241 ggatgggccc ttcaacgatt tccgtggaac ggtggcggaa gtgaatatgg aacgctcgaa
    12301 gattcgcgtc atggtgaact tcttcgggcg cgaaacccct g
//
LOCUS       DAOQAD010000053        12309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_5771_length_12309_cov_5.960503, whole genome shotgun sequence.
ACCESSION   DAOQAD010000053 DAOQAD010000000
VERSION     DAOQAD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5771_length_12309_cov_5.960503"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..879)
                     /gene="deoC"
                     /locus_tag="PKL60_05570"
     CDS             complement(49..879)
                     /gene="deoC"
                     /locus_tag="PKL60_05570"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNW95636.1"
                     /translation="MTKKTSDKAYDKSGSYEGFRVQTHFDKRGRLKEIGVENEMAAAA
                     PVPAIAALIDHTLLKPESTAEQILQLCEEATKYHFGAVCVNSCWVGLAAQKLAGTPVK
                     VGSTIGFPWGTASTAAKVAETRQAIADGAREVDMVLAYGKLKSCDYAAAAEDIREVVR
                     LAHKHDVLVKVILETSMLTQDEKIIACLLAKEASADFVKTSTGFQGGGATAEDIALMR
                     AVVGPDLGVKASGGVRSYADAKKMIAAGATRIGTSAGIKIVQEAAGEAAGTRAGEGEY
                     "
     gene            complement(1051..1377)
                     /locus_tag="PKL60_05575"
     CDS             complement(1051..1377)
                     /locus_tag="PKL60_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95637.1"
                     /translation="MPQKVILVSCGTGVVTAETTALRIRKIAAERKWQVEVRVVEFRK
                     LAQAAAGADVIVQIAPHDSTDYGIPKLSGLPFLTGFELDKTVQELEEILNPPDKPKPE
                     PTENGL"
     gene            complement(1424..1924)
                     /locus_tag="PKL60_05580"
     CDS             complement(1424..1924)
                     /locus_tag="PKL60_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNW95638.1"
                     /translation="MPPSIEPASTEPEMLRPELVFVGLQVGSREEALSFLSRRMLEAG
                     LVSPGFPAALLEREKAFPTGLQFAEAALALPHTETRYVLQPALAVAALAEPVEFLAMG
                     APEQSLPVRVIFLPAVLHPDWQVRWLSRLVALFQTPGFLTGIIQSPNRDRLYQSLRAA
                     FRNAPL"
     gene            complement(2090..2860)
                     /locus_tag="PKL60_05585"
     CDS             complement(2090..2860)
                     /locus_tag="PKL60_05585"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNW95639.1"
                     /translation="MGAFGSIDLNSKKPYYLQVKELIQTSIECGSFKPDEKLPSETEL
                     CSELRVSRTVIRQALTELEQLGLIRKRKGKGSFVSERKTVLAASLFLPDFLWDDDGER
                     EKISIKILTRQTIPAPARVALCLNMTEGEPILQFQRMYYHANVPIILVQIYLPTRIAS
                     QLSDEDLLLSMRDLDKVPAEIRFDHADQYLEIAYALKDEAELLQIPIGMPLIKVTTIS
                     YNAANTRIGVDITLMRADRIRLRGTIFKHSGQNRRQSA"
     gene            3141..4112
                     /gene="mvaD"
                     /locus_tag="PKL60_05590"
     CDS             3141..4112
                     /gene="mvaD"
                     /locus_tag="PKL60_05590"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNW95640.1"
                     /translation="MIATAIAHPNIAFIKYWGNRDARLRLPLTGSFSMTLAGLETRTR
                     VQFDADFPVDRFALNGEEQQNENLARVSGFLDRVRELAGISLFARVESGNNFPTGAGV
                     ASSASAFAALALAASSAAGLELTEPALSALARRGSGSACRSVPGGFAEWLPGSSDADS
                     YAVQVAPPEYWDLVDLIVILHSGHKAIGSTEGHALAGTSPLNALRVAGAPERLIECRQ
                     ALLGRDFERLARVVEADSHWMHAVMRTSSPPLFYWTPDTETVLWHVLTWRKQGRALCA
                     TVDAGPNVHVITTGAEKDWAEARLRALPGVLDVLTAKPGGGAKIVRI"
     gene            complement(4145..4999)
                     /locus_tag="PKL60_05595"
     CDS             complement(4145..4999)
                     /locus_tag="PKL60_05595"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HNW95641.1"
                     /translation="MDLKSAKTISEQIELLQERGIIIDDFDLASEFLECNNYYRLNIY
                     FHKLMDQPNHFTEGTAFSQIVNIYENDKWFRSELLLLLEPIEIKVRTQIAYYLGLAYG
                     PDAFYNEKIYKSIQRQGEIMGTFSKEIQRNPEDPVVKHHFEKYEGKFPIWVIVEYLSF
                     NTLSKYYKNLAESNKKIIAKNSFDLNEHLLGQWMHVLSVLRNICAHYGYLFRREYTLR
                     PIIARAFKWNSKANYQLFAQLLVTKRLSEPQTWKRFIVSLENKIPAGKDFSTHDYGFP
                     KNWQSFLS"
     gene            complement(5198..5956)
                     /locus_tag="PKL60_05600"
     CDS             complement(5198..5956)
                     /locus_tag="PKL60_05600"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNW95642.1"
                     /translation="MSFNILCANCGDGINAWEQRKTLVLDRILAFTPDLLGLQECRAD
                     GQAPYLIENLPGYTFFGVPRGGEGTTALEMAPIMLRKELFELLDVGDFWLSDQPGEPG
                     SRSWGGQFPRTVSWARLRVRENGQELLFANTHFDLVPAARRHSTELIRAWLEAQTAGC
                     PVILTGDFNAHKRSRVYQTLTRDGFLTDAWRQANLCARDQATFHGFGNPKARTCIDWV
                     LVSPSLHVLSASVDTYMQAGRYPSDHYPLRVEIE"
     gene            complement(6292..7539)
                     /locus_tag="PKL60_05605"
     CDS             complement(6292..7539)
                     /locus_tag="PKL60_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNW95643.1"
                     /translation="MDLVQEIKTKVEQNKSDMIQFLRDICAIPSTDSLIGPVGERIQA
                     EMNKLGYEEVRFDKMGNTFGRIGSGPRVLLYDSHIDTVGVGDISNWEWDPFVGKVENG
                     NFYARGACDEKGSTPGMIYGLAIARDLGLLDGYTVYYFGNMEEWCDGIAPNAFVEVDP
                     GVRPDFVVIGEPTRMNVYRGHKGRIELKIVATGRSAHAATHYLGDNAVYKMHALISEI
                     QELDRRMRFGMFSHPVLGHASITVTDAFARTNSVNAVPNQFTIFIDHRVTLSESRETA
                     LKTVRGLIADYMHDEITVEELVYDTPSYTGFSFPLDKFFPAWFAEESHPFVQAGQRAI
                     KDLWGVSRPSGTWDFSTNGTYWAGKANIPSIGFGPGNEVDAHTIGEHVPLQDVVDAAA
                     FYALLPKNLSDIIDYYRQFAGMK"
     gene            complement(7824..8807)
                     /locus_tag="PKL60_05610"
     CDS             complement(7824..8807)
                     /locus_tag="PKL60_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNW95644.1"
                     /translation="MMQTFLHGRDLIGDLDFSKEEVETVLDVAWDLKRKRALNEPHPY
                     LRDKVLAMLFFFSSTRTRGSFEAGISQLGGHAGFIESKSTQIAHGDTPKEIGEIFGRY
                     YDGIAIRHCDWEVGNAYINDVARASRVPVLNMQCDIYHPFQCLADLMTIMEKKGRDLR
                     KKKIVVSWAYAASYLKPISVPQSLILQMPRFGLDLTLSYPEEFRLMPEIEQQARDEAK
                     KAGTSFEIVHDMDEGMKGADIIYAKSWGPLLTTADGAEGKRIQDSYKSWLMDERRLAL
                     AKEDAIYMHPLPADRNIEVTDAVLDGAHSVVFDEAENRLHAQKAVMALTMS"
     gene            complement(8847..9800)
                     /gene="pyrB"
                     /locus_tag="PKL60_05615"
     CDS             complement(8847..9800)
                     /gene="pyrB"
                     /locus_tag="PKL60_05615"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNW95645.1"
                     /translation="MRSFCGRDILSLKDFERNEFFRVFEVAEELEPIARSRRNSDLLA
                     EKTLVTAFYQPSTRTRLAHEAAMHRLGGHVTGFSDAKMTRAGDFYQESIKDTVKMLEY
                     YGDVIVMRHFQQGAPHEAAKWASIPIINGGDGWGEHPTQILTDLYTVLREKGRIDGLK
                     WLAVGDMRMRTMHSLAYALTQFDCPITFVAPPEMALTPEFKEELKTYSLNFKEAEHVE
                     QVIAEADVILVEPVVQPDYTKHRDVRGEEVGLTPDNYKITRELLTNKAKSDAILLHSL
                     PRMDEIPTDVDITRWSRYWQEAFNGVVMRMALLALVLGKME"
     gene            10140..11180
                     /locus_tag="PKL60_05620"
     CDS             10140..11180
                     /locus_tag="PKL60_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNW95646.1"
                     /translation="MDLLSSIPNPSFMNKPGMNWGILGTARIAGNSVIPAIKANKHNT
                     VYAVASRDLTRAADFVRENGVPKAYGSYEELLSDANIQAVYIPLPNSEHGRWAIRAME
                     AGKHVLVEKPFAMNADEAQLMISTALEKSVVLMEAFMYRYHPRLAQMLEIVRKGTLGK
                     LRMLQTTMSFPLTNPDDIRLNAALGGGALYDLGCYCVDLQRILVGREPRRVQALGYQG
                     NTGVDLQVSASFDFGDQVYAQFDVGFNAARQQHIRIVGAEGVLDVEDPFGAKPEVSPA
                     YLTRDGETKKLTYKRDNAYEKMIEHFYNVVVSREAPLFPLANSVSNLVVIDALFRSLN
                     DGGRLVSLSPIN"
     gene            11196..11723
                     /locus_tag="PKL60_05625"
     CDS             11196..11723
                     /locus_tag="PKL60_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HNW95647.1"
                     /translation="MSDKKHSDTSLAARLGGYYLPTLLIIGLVLILTTGLKNPVGEIQ
                     NSLPHEDMLLKVVGYSALFCEVAAVFVIVTGTAHAIVSYTRRLFDRSLIDQIEGSEAI
                     RLRLGHKLSLSLEFAMASDILRLAVSPSFSSIIILFAIVLLRVLLTYFLEHDIEVIRE
                     SEMLSELQELREKDK"
     gene            complement(11797..>12309)
                     /locus_tag="PKL60_05630"
     CDS             complement(11797..>12309)
                     /locus_tag="PKL60_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNW95648.1"
                     /translation="TNTATHTPTITETPTITPTYTPSAPFEYVVQEDDNCAVIAEKYD
                     VDVEVLLYLNNLDSSCIINVGQTILIPAPGQELPTATPLPTDIRRGTVIEYKVRSGDT
                     LEALAIRFNSTVDKILYETNRWRRTQGLTDLKDSSNLFIGDILKIPVNLVTPVPTATA
                     TRTMTPSPTR"
BASE COUNT         2605 a         3344 c         3456 g         2904 t
ORIGIN      
        1 gcgctgcaat catctcaatt ctcaacaagt ggaaggcacc cgcccaggct aatattcgcc
       61 ctccccggcc cgcgttccag cagcttcccc agcggcctcc tgcacaatct tgatcccggc
      121 gctggtgccg atgcgcgtcg ccccggcggc aatcatcttc ttcgcatccg cgtagctgcg
      181 caccccgccc gaagccttca cccccaaatc cggcccgacc accgcgcgca tcagggcgat
      241 atcctcagcg gtcgcgccgc cgccctggaa gcccgtagag gtcttgacga aatccgcgct
      301 ggcttctttg gccagcaaac acgcgatgat cttttcgtcc tgggttagca tgctggtctc
      361 cagaatgacc ttgacgagca cgtcgtgctt gtgcgccagg cgcaccacct cgcggatatc
      421 ctcggctgcc gcggcataat cgcaggattt cagtttgcca taggccagca ccatatccac
      481 ctcgcgcgcg ccgtcggcaa ttgcctggcg ggtctcggcg accttcgcgg cggtggaggc
      541 agtgccccag gggaagccaa tcgttgaccc cactttcacc ggcgttccgg ccagcttttg
      601 ggcagccagc cccacccagc aggaattcac gcacaccgcg ccgaagtggt actttgtcgc
      661 ttcctcgcag agctgtaaaa tctgctcggc agtggattcg ggcttgagca gggtgtggtc
      721 aatcagcgcg gcgatggctg ggacaggggc ggccgcggcc atttcgtttt ccaccccaat
      781 ttctttcagg cgcccgcgtt tgtcgaagtg agtctggact ctgaacccct catagcttcc
      841 gcttttatca taggctttat cgctggtctt tttggtcatt ttttcctctt tttagtttga
      901 gtgcaatctc caccgcggga agaccgcgct cccaatcaca agccgaatca ggagtcgagc
      961 gcacgaatcc aatcatctcc aggcaaccaa ctgccgccgg ggcaaggctc aggcggacaa
     1021 taaaaacccg actgctgtgc tccacgctga ttatagcccg ttttcagtgg gctctggttt
     1081 gggtttgtcc ggcgggttca ggatttcttc cagctcttga acggttttat ccagctcgaa
     1141 gccggtcagg aagggcagcc cactcaattt ggggatgccg taatcggtgc tgtcgtgcgg
     1201 agcaatctgc acgatgacat ccgcgccggc agcagcttgc gccaactttc ggaattcgac
     1261 cacccggact tccacctgcc atttgcgctc ggcggcaatt ttacggattc gcagcgcggt
     1321 tgtttcagcc gttaccacgc ctgttccgca ggagaccaaa ataacttttt ggggcatcgt
     1381 tcacctccgt gagttttctt tgaatcctat caaaaatcag cgtttagagg ggagcgtttc
     1441 taaaggctgc gcgcagagat tgatagagac gatcccggtt aggggattgg atgattccgg
     1501 tcaaaaaacc aggggtctgg aaaagagcga ccagccttga gagccaacgc acctgccaat
     1561 ccgggtggag gacagcgggc aggaagataa cccgcacggg caggctctgc tccggcgcgc
     1621 ccattgccag gaattccact ggctctgcca gcgctgccac agccaacgcg ggctggagca
     1681 cgtaacgcgt ttcggtgtgc ggcagagcca gcgccgcctc ggcgaactgc agaccggtgg
     1741 ggaaggcttt ctcccgctcc agaagcgccg caggaaagcc gggcgagacc agccccgcct
     1801 ccagcatgcg ccgtgacaga aacgagagcg cttcttcccg gcttccaacc tgcagcccga
     1861 cgaacaccaa ctccgggcgg agcatctcag gttccgtgct tgcgggttcg atagagggcg
     1921 gcatcttaat ttctactccc gttgagatgg aaggcactga aaaaactcat ttcgaaggta
     1981 agaataatcc gcttgattct ttgtgtcaat tggcacggaa gcgaacctat cccatcggaa
     2041 caaggcggaa tgcagggaaa aaggcctgaa cagcaggctg ccgccgctgc tacgcggatt
     2101 ggcggcggtt ctggccgctg tgtttgaaga tggtgccgcg caggcggatc cggtctgcgc
     2161 gcattaaagt aatatcgaca ccaatccggg tgttcgctgc gttataactg atggtggtaa
     2221 ctttgattaa gggcattccg atcgggattt gcagcagctc tgcctcgtct ttcagcgcgt
     2281 aggcaatttc caggtactga tcggcatggt caaagcgaat ttcggccggg accttatcca
     2341 ggtcccgcat cgacagcagc aagtcctcat ccgaaagctg gctggcaatg cgcgtcggca
     2401 ggtagatttg caccagaatt attggcacat tagcgtggta gtacatgcgc tggaactgca
     2461 ggatcggttc gccttccgtc atattcagac acagcgcaac acgcgcgggg gcgggtatcg
     2521 tttgcctggt gagtattttg atgctgattt tttccctttc accatcatca tcccacagaa
     2581 agtccggcag gaagagggaa gcggccagga cagtcttcct ctcagagacg aacgagccct
     2641 tccctttgcg cttgcggatc aggccgagct gctccagttc ggtcagcgcc tggcggatga
     2701 ctgtgcggct gactcttagc tcgctgcaga gctcggtttc gctgggcagc ttctcatcgg
     2761 gcttgaatga gccgcactcg atgctcgttt gaatcagttc ctttacttga aggtaatagg
     2821 gtttcttgct attcaggtca atgcttccaa atgcccccac attatctcgc tttcattggg
     2881 tttcaattaa gtttcgatta tgttcgtccg gtagattata ccttaaaacc cggcgattcg
     2941 gataaaaaac cataaaaaaa ccatacaatt cctgcctcat cccctttcat tttagaaaac
     3001 ttatgagcgc cggtttccac gggaatacca gccgcccgag tagcgccagc agctgaagcg
     3061 cggcgctgcc tttacccggg aaaacgcgcg cccacggagg atgaaaaaag gtaaaatcgt
     3121 attattccaa agaggtcccc atgattgcta ccgctattgc ccacccgaac atcgctttta
     3181 tcaaatactg gggcaaccgc gacgcgcgcc tgcgcctgcc cctgaccggt tccttctcga
     3241 tgaccctcgc tggccttgaa acccgcacgc gcgtgcagtt tgatgccgat tttccggtgg
     3301 accgcttcgc gttaaacggg gaggagcagc agaatgaaaa cctggcgcgg gtttccggct
     3361 ttttggaccg tgtgcgggag cttgccggca tttcgctctt cgcgcgcgtg gagagcggga
     3421 acaacttccc gaccggcgcc ggggttgctt cttccgcctc ggcgtttgcc gcgctggcgc
     3481 tggcagccag cagcgcggct gggttggagc tgacagaacc cgcgctctca gccttggcgc
     3541 ggcgcggctc aggctcggct tgccgctcgg tgcccggcgg atttgccgaa tggctgcccg
     3601 gaagcagcga cgcggacagc tatgctgtgc aagtcgcccc accggagtat tgggacctgg
     3661 tggacctgat tgttatcttg cactcggggc ataaggcgat tggctcgacg gaagggcacg
     3721 cgctggctgg cacctccccg ctgaatgccc tgcgcgtagc cggcgcccct gagcgcttga
     3781 ttgagtgccg tcaggcgctg ctggggcggg attttgagcg cctggcgcgc gtggtggaag
     3841 cagattcgca ttggatgcac gcggtgatgc gcaccagttc cccgccgctt ttttactgga
     3901 cgccggacac ggaaacggtg ctgtggcatg tgctgacctg gcgcaagcag gggcgcgcgc
     3961 tgtgcgccac agtggatgcc ggacccaacg tgcatgtcat cacaacgggt gcagaaaaag
     4021 attgggcgga agcccggctg cgcgccctgc cgggagtgct ggatgtgttg actgcgaagc
     4081 ctggcggcgg ggcgaaaatt gttcgcatat aaatgattaa gtagttactg taaaattagt
     4141 aaaattaaga taaaaagctt tgccaatttt tgggaaatcc ataatcatga gtagaaaaat
     4201 cttttccagc tggtatctta ttttcgagac taacaatgaa cctcttccag gtttgtggtt
     4261 ctgataatcg ttttgttaca agaagctgcg caaataattg atagtttgcc tttgaattcc
     4321 atttgaaagc tcgcgctatt ataggcctta atgtatattc ccttcgaaac aaataaccgt
     4381 aatgtgcgca tatgttccta agaactgaaa gcacgtgcat ccattgtccc aaaagatgct
     4441 catttaaatc aaaagaattt tttgcaataa tttttttatt gctttctgct aaatttttat
     4501 agtattttga taaagtattg aatgataaat actcgacaat cacccagatt ggaaatttcc
     4561 cctcgtattt ttcaaaatgg tgttttacaa caggatcctc ggggtttcgc tgtatttctt
     4621 tggaaaaagt acccattatt tctccttgcc tctgaatact tttgtaaatt ttttcattgt
     4681 aaaaagcatc tggtccatat gctaggccta agtaatatgc aatttgagtt cgaaccttaa
     4741 tttcaatcgg ttcaagtaac aataagagct ccgaacgaaa ccacttatcg ttttcgtaaa
     4801 tattaacaat ctgagaaaat gcggtccctt ctgtaaaatg gtttggttga tccattagtt
     4861 tatgaaaata aatattcaac cggtaataat tattacattc taggaattca gatgctaaat
     4921 caaaatcatc aatgattatt cctcgctctt gtaataattc aatttgttct gaaattgtct
     4981 tagctgattt caaatccata aatctacctt attaaaaaat aatgacccac ctcggtgcgc
     5041 ataatacatt ctctttcgag tttataatag aggcgtggtg ggtactgttg aatttagtat
     5101 acatcttcag tttatagaag tcaagattaa tttattccac aatcatataa cctattccac
     5161 aatcatataa cctagaatga tttctaagtt aattttatca ttctatctcc acccgcagcg
     5221 gatagtgatc tgatgggtag cggccggctt gcatgtaagt atctaccgag gcggaaagca
     5281 catgcaagct tggcgatacc agcacccaat caatgcaggt ccgggctttg gggttgccaa
     5341 aaccgtgaaa ggtggcctga tcgcgggcgc agaggttggc ctggcgccag gcatctgtga
     5401 ggaagccgtc tctggtaagg gtctgataga cccgtgagcg cttatgagca ttgaagtccc
     5461 cagtaagaat gacgggacag ccggcagttt gggcttccag ccaggcgcga atcaattcgg
     5521 tggagtggcg tctggcggct gggaccaggt caaagtgggt atttgcgaaa agcagttcct
     5581 gcccgttttc tcttacgcgt aaacgagccc aactgactgt gcgcgggaac tggccacccc
     5641 agctgcggct gccgggttcg ccgggctggt cgctcagcca gaaatcaccg acatccagca
     5701 gctcaaaaag ctctttgcgc aacatgatgg gcgccatctc aagcgccgtg gtgccctcac
     5761 cgccgcgcgg cacaccaaaa aaggtgtatc caggcaggtt ttcaatcagg tacggagctt
     5821 gaccatccgc acggcactcc tgcaggccga gcaaatcggg cgtaaacgcg agaatgcggt
     5881 cgagaaccag agttttgcgc tgttcccaag cgtttatccc atcgccacag ttagcgcaaa
     5941 ggatattgaa actcattacg cgcagcatgc cctgattatg ccatgaagcc gctggttata
     6001 tacaaataat actgcggtaa tctgcggctt caataactat ggatacactc acccaggaga
     6061 ggcagataga ttacggcaag gccagccgct atcgatccct gtttcggaca catcctgcct
     6121 tccgtcccgg cggtaattgc cgatgaagtg gatctcgcgc ggatagtgtt cccgaatgac
     6181 atgctcgtac cacgaaattt cgtcatccac ctcgccagcg tgcacgcgtc cgcctgctga
     6241 gcgggtgttg ttgcagtcga tccaatccgg gtcccattcg caaagtgcgg cttatttcat
     6301 gcccgcgaac tgcctgtagt aatcgataat gtcgctcagg ttctttggca gtagcgcgta
     6361 aaacgcggcg gcgtccacca catcctgcag cgggacgtgt tcgccaattg tgtgcgcgtc
     6421 cacctcgttg ccggggccga aaccgatgga gggaatgttg gctttacccg cccagtaggt
     6481 gccgttggtg gaaaaatccc aggtgccgga ggggcggctg acgccccaga ggtccttgat
     6541 ggcgcgctgc ccggcctgca cgaaaggatg cgactcttcc gcgaaccagg ccgggaagaa
     6601 tttgtccagc gggaagctga agccggtata ggagggcgtg tcgtagacca gctcctcgac
     6661 cgtgatttca tcatgcatgt agtcggctat cagcccccgg acggttttca aagctgtttc
     6721 gcgggattcg ctcagggtaa cgcggtggtc gataaaaatg gtgaactggt tggggacggc
     6781 attcacggaa ttcgtgcgcg cgaacgcgtc cgtgacggtg atcgaggcat ggccaagcac
     6841 cgggtggctg aacatgccaa agcgcatgcg ccggtccagc tcctggattt cggagatgag
     6901 agcgtgcatc ttgtagaccg cgttgtcccc caggtagtgc gtggcggcat gcgccgaacg
     6961 cccggtggcg acaattttca gctcgatgcg gcctttatgt ccgcggtaga cgttcatcct
     7021 ggtcggttcg ccgatgacga cgaaatcagg cctgacgccg gggtcaacct cgacgaaggc
     7081 gtttggcgcg atgccgtcgc accattcttc catgttgccg aaataataga cggtatagcc
     7141 gtccagcagg ccgaggtcgc gggcgatcgc caggccgtaa atcatgccgg gggtgctgcc
     7201 tttttcgtcg caggcgccgc gggcgtagaa gttgccgttt tcgaccttac cgacgaacgg
     7261 atcccactcc caattgctga tatcgccgac gccgactgtg tcgatgtgcg agtcgtacaa
     7321 cagaacgcgc ggaccgctgc cgatgcgccc aaaggtattg cccattttgt cgaagcgcac
     7381 ctcttcatag ccaagcttgt tcatctctgc ttgaatacgt tccccgacgg gaccgatcag
     7441 cgagtccgtg gaggggatgg cgcaaatatc gcgcaggaac tggatcatat cgcttttgtt
     7501 ctgttctact ttggttttta tttcctgaac aagatccatg agtgcagccg ctcctttata
     7561 ttcctcaacc ccgggcacgc gcctgaagga ggtgagtttc ccaatcattg atgaaagcag
     7621 cccagcctgg aggctcctgg ctgcagggac tatgataaca tacattcccc gcggggcagt
     7681 tcccgggctg aaatcgctcc tttcaggata gacacgtgcg ccatccagct ggcaccaatc
     7741 ccctggcagt tgataggttc ctgcttctgc agtgtaaaaa aaaggcgggt tttcgcccgc
     7801 cccaaactat tttctatccg gattcaagac atggtcagcg ccatgaccgc cttttgggcg
     7861 tgcaggcggt tttcggcttc gtcgaacacc accgagtgcg ctccgtccag caccgcgtcg
     7921 gttacttcga tattgcggtc agcgggcagg gggtgcatgt agatggcgtc ttccttcgcc
     7981 agagccaggc ggcgttcgtc catcagccag gatttgtagc tgtcctggat gcgtttgcct
     8041 tcagcgccgt ccgcggtggt cagcagcggc ccccaggact tggcgtagat gatatccgcg
     8101 cctttcatgc cctcgtccat atcgtgcacg atttcaaagg aggtgccggc ctttttggct
     8161 tcgtcgcgcg cctgctgctc aatttccggc atcaggcgga attcctccgg ataggaaagg
     8221 gtcaggtcca gcccgaaacg cggcatttgc aggatgagcg actgcggcac ggagatgggt
     8281 ttgaggtacg aagccgcata cgcccaggag accacaatct tctttttgcg caggtcgcgg
     8341 ccttttttct ccataatggt catcaggtct gccaggcact ggaaggggtg ataaatatcg
     8401 cactgcatgt tgaggacggg gacgcgcgag gccctggcga cgtcattgat gtaggcgttg
     8461 ccgacttccc agtcgcagtg gcggatggcg atgccgtcgt aatagcggcc aaagatttcc
     8521 ccgatttcct tgggggtatc cccgtgggcg atttgcgtgg acttgctctc gatgaaaccc
     8581 gcgtgcccgc ctaactggga aatgccggct tcaaaggagc cgcgcgtgcg cgtgctgctg
     8641 aagaagaaca gcattgccag cactttatcg cgcaggtatg ggtggggttc gttcagggcg
     8701 cgcttgcgct tcaggtccca ggcgacgtcc agaacagtct caacttcctc tttgctgaaa
     8761 tcaagatcgc caatcaggtc tctaccgtgt aaaaaagtct gcatcattct ctccttggaa
     8821 aaatggtatc gtgtgcggtt tcgggtttat tccatcttgc ccaacaccaa tgctaacaga
     8881 gccatgcgca tcacgacccc gttgaaggct tcctgccagt aacgcgacca acgcgtgatg
     8941 tccacgtccg tcggaatttc gtccatgcgc ggcagggagt gcagaaggat ggcatcggat
     9001 ttcgccttgt tggtcaacag ctcgcgggtt attttatagt tatcaggagt taaccccact
     9061 tcttcgccgc gaacatcgcg gtgcttggtg taatcgggct gcacgaccgg ctcgaccagg
     9121 ataacatccg cctcggcgat gacctgctcc acatgttcgg cttccttgaa attcaggctg
     9181 taagttttga gctcttcttt gaattccggt gtcagcgcca tttccggcgg ggcgacgaag
     9241 gtgatagggc agtcgaactg ggtgagcgcg tacgccaggg agtgcatggt gcgcatgcgc
     9301 atatccccga ctgccagcca cttgaggccg tcgatgcggc ctttttcgcg caagacggtg
     9361 tacaggtcgg tcaggatctg ggtcgggtgt tcgccccagc cgtcaccgcc gttgataatc
     9421 gggatggagg cccatttggc ggcttcgtgc ggcgcgcctt gctggaaatg gcgcatgaca
     9481 atcacatcgc cgtagtattc cagcatttta accgtatcct tgatggactc ctggtaaaaa
     9541 tcgccggctc gcgtcatctt ggcgtccgaa aagccggtga catgcccgcc caggcggtgc
     9601 atggccgcct cgtgcgccag gcgcgtgcgc gtgctgggct ggtagaaggc ggtgaccagc
     9661 gttttttctg ccagcaaatc cgaattgcgc cgcgagcggg cgataggttc cagctcttca
     9721 gcgacttcaa agaccctgaa aaattcattt cgttcaaaat ctttcagcga caaaatatcc
     9781 cgaccacaaa aactacgcat aatttctcct ttaattcggt tttctatcta gattagcggg
     9841 tgagaacagt tcagaactcc agatgggaaa ataccaggcg ggaattcggg cggctgcccg
     9901 gcaggcacag gcggggaaag cacgacctgc agtctgacct ggcggtttcg catccatagc
     9961 atcaattata aatctcatcc gggtggctga caattttcag cattcattcc tgcaaaattc
    10021 agcgctgaat tggatggaaa acgggagact tgcgcgaaaa tctgcgcttg ggatttcagc
    10081 gggcagggta taatgatttt gcgttgatct gttgaatttc tattcaaaag aggtggcaaa
    10141 tggatttgtt atcttcaatt cccaatcctt cgttcatgaa caaacccggg atgaattggg
    10201 gcatccttgg cacagcccgc attgctggca attctgtcat ccccgccatc aaggcgaaca
    10261 agcataatac cgtttacgcc gtcgccagcc gggacctgac gcgcgcggca gactttgtgc
    10321 gcgagaatgg ggttcccaaa gcctacggct cttatgagga actgctcagc gatgcgaaca
    10381 tccaggcagt gtacatcccg ctgcccaata gtgagcacgg ccgctgggca atccgcgcga
    10441 tggaagccgg caaacacgtg ctggtggaaa agcctttcgc gatgaatgcc gacgaagctc
    10501 agctgatgat cagcacagcg ctggagaaat ccgtggtgct gatggaagcg tttatgtacc
    10561 gctatcaccc gcggctggcg cagatgctcg aaattgtgcg caagggcacg cttggtaagc
    10621 tgcggatgct tcagaccacg atgagttttc cgctgacaaa ccccgatgac atccgcctga
    10681 atgccgctct gggcggcggc gcgctctatg accttggctg ttactgcgtg gatttgcagc
    10741 gcatcctggt ggggcgcgaa ccccgccgcg ttcaagcgct cggctaccag gggaacaccg
    10801 gcgtggacct gcaggtctcg gccagcttcg attttgggga tcaggtgtat gcccaatttg
    10861 atgtcggctt caacgcagcc cggcagcagc atatccgcat cgttggcgcc gagggcgtgt
    10921 tggatgtgga ggatccgttt ggagccaaac cggaagtcag tccggcttac ctgaccagag
    10981 atggcgaaac caaaaaactt acctacaaac gcgataacgc ttatgaaaag atgatagaac
    11041 acttttacaa tgtggtcgta agccgcgaag cccccctgtt tccactggca aactctgtca
    11101 gcaatttggt tgtcattgac gcgctgttcc gatccctgaa tgacggcggc cgattggttt
    11161 ccttatcccc cattaactga tccatgaggt aaagtatgtc cgacaagaaa cattccgata
    11221 catctctggc agccaggctg ggcggctact acctgcccac cttgctgatc atcggcttag
    11281 tcctcatcct caccaccggt ctgaaaaatc cagtcggcga aatccaaaac agcttgccgc
    11341 atgaagacat gctgctaaaa gtagttggct attcggctct gttttgcgag gttgccgctg
    11401 tctttgtaat cgtgaccggg acggcgcatg ccattgtcag ctatacccgg cgattattcg
    11461 atcgctcgct gattgatcaa attgaggggt ctgaagccat tcgtctgcgt cttggccaca
    11521 agctcagcct cagcctcgaa tttgccatgg cttccgacat cctgcgtttg gcggtatcgc
    11581 cctcgttttc ctctatcatc atcctcttcg cgattgttct gctgcgcgtg ttgctgactt
    11641 atttcctgga gcatgatatc gaagtgatcc gcgagagcga gatgctttct gaattacagg
    11701 aattgcgcga aaaggacaag tagccggcat tctttcagag ctgtaaaaaa aaaggatacg
    11761 cggagcgtat ccttttcatt tcaaatgcgc gcgggcttac cgggtcggtg agggcgtcat
    11821 cgtgcgggtc gcggtcgcgg tcgggacggg ggtgaccagg ttcacaggga tcttgaggat
    11881 atcgccgatg aagaggttgg agctgtcttt caggtcggtg agaccctgag tgcggcgcca
    11941 gcggttggtt tcgtacagga ttttgtccac ggtggagttg aagcggattg ccagggcttc
    12001 cagggtgtct ccggagcgga ctttgtattc gatgaccgtg ccgcggcgga tatcggttgg
    12061 cagcggggtg gcggtgggga gctcctgccc gggcgcgggg atcagaatgg tctgcccgac
    12121 gttgatgatg caggagctgt ccaggttgtt caggtagagc agcacttcca cgtccacatc
    12181 gtacttttcg gcgattaccg cgcagttatc gtcctcctgc acgacgtatt cgaacggcgc
    12241 ggagggggtg taggtggggg tgattgtggg cgtttcggtg atcgtcggcg tatgcgtcgc
    12301 ggtgttggt
//
LOCUS       DAOQAD010000054        12091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_5924_length_12091_cov_6.038800, whole genome shotgun sequence.
ACCESSION   DAOQAD010000054 DAOQAD010000000
VERSION     DAOQAD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5924_length_12091_cov_6.038800"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKL60_05635"
     CDS             complement(<1..229)
                     /locus_tag="PKL60_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95649.1"
                     /translation="MTQPNYPAMQGPVCPLPLQPGGQIVIGHGSGGGMTRDLIVNVFQ
                     KYLGNAILNAGNDSALLSGGAALVFNRSDDIT"
     gene            complement(226..1323)
                     /gene="hypD"
                     /locus_tag="PKL60_05640"
     CDS             complement(226..1323)
                     /gene="hypD"
                     /locus_tag="PKL60_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNW95650.1"
                     /translation="MSLAATEGFRSAGLTRQLAGEIRRLVTRPWNIMEICGGQTHAIL
                     QYGLDQLLPPEITLIHGPGCPVCVTSLDAIENALQIARQPEVVFTSFGDMLRVPGAHA
                     NLFSARAEGADVRVVYSPLDAVKLAQENPQKQVVFFAIGFETTVPSIAASILHAERNR
                     INNFSILPANVLVPPAMHAILKSPDNRVNAFLAAGHVCAVMGYWEYEPIAAQYRTPIA
                     VTGFEPVDLMRGILMCVRQLESARFEVENAYSRAVTRAGNRAAQDMISQVFEPVDRQW
                     RGIGVIPLSGLGLRPDYSDFNALKRFRLVQQAVSESPLCIAGQVLRGNARPPDCPAFR
                     KECTPASPLGAPMVSSEGACAAYYRYQRVSA"
     gene            complement(1320..1583)
                     /locus_tag="PKL60_05645"
     CDS             complement(1320..1583)
                     /locus_tag="PKL60_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNW95651.1"
                     /translation="MMCLGIPGMITRLYDLDGTAMAMVDFGGVQQEVCVATVPEAQVG
                     QYVIVHAGFALNLLSEEEALETMRLLKEIKEFNQAESKGKTEE"
     gene            complement(1595..3886)
                     /gene="hypF"
                     /locus_tag="PKL60_05650"
     CDS             complement(1595..3886)
                     /gene="hypF"
                     /locus_tag="PKL60_05650"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNW95652.1"
                     /translation="MKLQNWRFHITGIVQGVGFRPTVYLLAQEHQLSGWVVNTARGVE
                     IEIHGSEEDCAAFTESLRNNPPALSKVDSFAVFPAPLQAYAEFTIRESENRAEDFLPV
                     SPDVAICADCRAELFNPKDRRYRYPFINCTHCGPRFSIVRQIPYDRANTSMAGFPLCP
                     DCAAEYHNPSDRRFHAQPVACPVCGPQIWYEENGARIQSGEQALQAARAALQDGKILA
                     VKGLGGYHLACDAHNALAVQTLRERKHRTGKPFALMAFSLEVVEKYVQLSDQERALLT
                     SPQAPIVLSETTAAGKELAKIAAPDQVRLGFMLPYTPLHLLLLEPAPGFPDALVMTSG
                     NLSEEPLAYRDEEAHQRLAPLADAFLTHNRPIHMRVDDSVVTALRGQVYFARRARGYA
                     PGQLHIPPAAQPVLGTGTQLKNTFCLARDQYAFVSHFIGDLENQETLESFEQAVGHYE
                     KLFRIHPEALACDLHPDYLATRYARARSQAESLPLIAVQHHHAHLAACLAENAWESRE
                     PAIGLIFDGTGYGTDGSIWGGEILVGGCASFERRFHLLPVRLPGGDLAVRKPARTALS
                     WLTAAGISAEEYKLPPIQALSTEERSVVRAQLESGYNAPLTSSMGRLFDAAASILGLC
                     HEISYEAQAAIALESAADPDEAGAYPFTLQDGIIDPREMLKELVRDLQGGLGVPRIAA
                     RFHNSITRLSVQACQLIREETGIFHVAISGGVWQNMRLMRALLPALEAEGFVALYHHA
                     LPTNDACVSLGQVMVALQALRES"
     gene            complement(3897..4559)
                     /gene="hypB"
                     /locus_tag="PKL60_05655"
     CDS             complement(3897..4559)
                     /gene="hypB"
                     /locus_tag="PKL60_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNW95653.1"
                     /translation="MTQRVEIVRRIMDSNQRVAAGNRALLDEHSVYAMNVMASPGAGK
                     TSFIMETLRRLPPGVRPGVIEGDTAPVTIDSDKISALGIPAVQINTGGDCHLDAVMVE
                     LALQKLDLEALNLVIIENVGNLICPAGFDLGTHANIVIASIPEGDDKPYKYPNIYRGL
                     DVLIINKTDLLPYIDFRMEYFSQGVEILNPGLKIFPVSCKTGEGFEAWMDWLTRTLPP
                     KA"
     gene            complement(4559..4909)
                     /gene="hypA"
                     /locus_tag="PKL60_05660"
     CDS             complement(4559..4909)
                     /gene="hypA"
                     /locus_tag="PKL60_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNW95654.1"
                     /translation="MHELAVTESILEIANRAALDNCASRVTDIYLTIGQLSSIVDDSV
                     QFYWDHISQGTPSAGAVLHFQRVPARLRCKSCGNEYELNEELMPCPNCGSIALEIMSG
                     EELLVDHIEVLKEE"
     gene            complement(5415..6176)
                     /gene="fdhD"
                     /locus_tag="PKL60_05665"
     CDS             complement(5415..6176)
                     /gene="fdhD"
                     /locus_tag="PKL60_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNW95655.1"
                     /translation="MTEPYQEKEFWQFSRASGKLLPARKPIVSEHAAALYVNDEPWLT
                     FICSPTDLDALALGFLYGENVIKDLGEVSSIQVGDGGSRIMVKLSRTAEKPTHFHRTS
                     TGYGLESSLRLPHLPTDFRITVEDVLALYLQFMDQQELHDQVGGFHSAGLSDGLRVPI
                     MVEDLGRHNCVDKLTGLFLLQNRPFTPNLLLLSGRISSEMVFKTFALGVPVIVSRTSP
                     TALAVETAWEAGITLIGYLRSAQFDVYSHPERLSL"
     gene            complement(6173..6706)
                     /locus_tag="PKL60_05670"
     CDS             complement(6173..6706)
                     /locus_tag="PKL60_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95656.1"
                     /translation="MNPPKKTLLIAYGNRDRQDDGAGWHILTDIAREFGAAAPELPGD
                     WVDAAEGALRLWYLYQLLPEISEDLTAYERVVFIDAHNSPQLPDLVFERTGPAWEHSA
                     FTHHMSVGELLSITTALFSACPECWLLSVRGNAFEFSQELSAPTKELAFSAAQMLIAF
                     LRGDGFSPQHPERKPSE"
     gene            complement(6769..8193)
                     /locus_tag="PKL60_05675"
     CDS             complement(6769..8193)
                     /locus_tag="PKL60_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNW95657.1"
                     /translation="MVAQKITIEPVTRIEGHAKVTIHMNDDGSVEHAYFHVNEFRGFE
                     KFCEGRLVAEMQQITPRICGICPVSHHLAAAKAGDVVLGCPPPRPASLLRELMHMGQI
                     IQSHGMHFFELAGPDLILGFDHDPATRNVVGLIQADPQLTLRAVRLRKFGQAIISELG
                     ERSVHPNFAIPGGVNRQFTQAGRDRLLAQVDEQIETIKIGIQIIHAWIEAHQTEIDEF
                     AVFPTGYLGLVTPRNSLELYDGDIRLTGRDGKELEKFDPQDYLDYIAEHVESWSYLKF
                     PYYKKLGYPEGVYRVGPLGRLNNSDQIDTPLANEEFKHFKALNGGKPVENTLHYHYAR
                     LIETLFAAEHARILLEDPDILSSDILNTRQQPQEQGVGIIEAPRGTLFHHYWSNGSGQ
                     LTKVNLIVATGNNNYAMSRAVDEVAKRYVHGPDITEGMLNRVEHAIRAHDPCLSCSTH
                     AVGSMPLEVSVFNKQGELVRVIRR"
     gene            complement(8202..8744)
                     /locus_tag="PKL60_05680"
     CDS             complement(8202..8744)
                     /locus_tag="PKL60_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNW95658.1"
                     /translation="MAKPKVASDWLAGCAGCHMSFLDIDERIVALTELVDLRATPITD
                     LKEPDESGVDVGILEGGISNTYNEEVAKKMRARCKILVALGDCAVFGGVPAMRNFEGA
                     EAALKRAYVETESTDESGKIPDDPELSTPVHVRPVSDVVKVDLFLPGCAPSADAIFYI
                     LSELAQGRMPEVSGEFNDWH"
     gene            complement(8780..10735)
                     /locus_tag="PKL60_05685"
     CDS             complement(8780..10735)
                     /locus_tag="PKL60_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNW95659.1"
                     /translation="MVTLTIDGIQVEVPDGTTVLEAAEKVGIKVPRLCHHPSLKPYGG
                     CRLCMVEVEGARILQPSCTLPTSNNMVVRTNTPKVQEARKFVLSMLFSERNHFCMYCQ
                     ATDGDCELQCAAYQQSLTHWPLTPNYSPYAVDASHKYIFVDNNRCILCRRCVRACGDL
                     VGNFTLGFEERGANSFLVADYGVPLGESTCISCGACVQICPTGALMDRRSAYQGRESD
                     LTPVVSVCSECSIGCQRNVLTRNNRLVRIDGVWNSEPGEGLLCEKGRYMPLESKAERL
                     SVPEILRDGKRVAVTWEAALTEAAQHLRAAADEAALFASAKLPIESLAALKSAFTGGL
                     RSANLLLSDDNEAALASVKLAEELHGPFEAKLEALKHVDVALLIGSDLENDNQVAGFF
                     LKRQVLENSRIISITTRQKPLIARSALRIQQKKGSSFDLVNGLVSAWHSLLHEGDKFA
                     EENIAHLEEKTGIKSAEILRAVTMLKDAQHPVVVLGGEFASLEHYDALVRLVLFCRQN
                     NFPVLIIKEQANSLAAAQLGYTTAADGKKVKAAFVALGDAEPTEALLKHCAGIPLLVV
                     SAAHPSALTERAAVVLPCAAWAEEGGSYLSTEGRLQQKLAALDAPESVKTSAAVADAL
                     VQALNARGGQDWKTALKQQPSPVDLDF"
     gene            complement(10779..>12091)
                     /gene="nuoF"
                     /locus_tag="PKL60_05690"
     CDS             complement(10779..>12091)
                     /gene="nuoF"
                     /locus_tag="PKL60_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNW95660.1"
                     /translation="IAWVAARKGTLPAETRIVLKNVGIIDPNSIEDYIANDGYQGLGN
                     ALSMTPEQVIALIKESGLQGRGGAGFPTGLKWGFVRQARADKKYVICNADESEPGTFK
                     DRVLIEGDPHQIIEAMLIAAYAVGATEGYVYLRGEYQLAIQRMETAIAQARSSGLLGN
                     FILGSSLSFNLHLHTGAGAYICGEETALIESIEGKRGEPRSRPPYPTTEGLWGKPSLV
                     NNVETLANVPSILRNGAAWYRKFGTPSSPGTKVYTLLGNLNVTGLIEAPMGITLREMI
                     TIYGKGIKDGHFKLAQTGGSGGSIIPASLQDTPMDFASFAKAGVVLGSGALLICNDSV
                     CVVDLARVLLNFFRKESCGKCSPCRICTQKAYEILTNISTGHGSLQDLDALREISVQL
                     NELSTCGLGQTAAVPLRDTLNHFRDEVEAHIRLKVCPAGVCSMS"
BASE COUNT         2450 a         3433 c         3635 g         2573 t
ORIGIN      
        1 aggtgatgtc gtccgaacgg ttgaagacca gggccgcgcc gccgctcagg agcgcgctgt
       61 cattgccggc gttgagaatc gcgttgccca ggtacttctg aaacacgttc acgatcaggt
      121 cgcgggtcat tccgccgccg ctgccgtgac cgatgacaat ctgtccgccg ggttggagcg
      181 ggagcgggca aacaggaccc tgcatagcgg ggtaatttgg ctgggtcatg cactcacccg
      241 ctgatagcgg tagtaggccg cgcaggcgcc ttccgaagaa accatcggag cgccaagcgg
      301 ggaggcgggt gtgcattcct tccgaaatgc cgggcagtca ggcggcctgg cattcccgcg
      361 cagaacctga ccggcaatac acagcggcga ttccgagacg gcctgctgga caaggcggaa
      421 gcgtttgagc gcgttgaaat ccgagtaatc cgggcgcagt cccaggccgg aaagcgggat
      481 gacgccgatg ccgcgccatt ggcggtccac cggttcaaaa acctgactaa tcatgtcctg
      541 ggcggcgcgg ttgcctgccc gggtaactgc cctggaatag gcgttctcga cctcaaaacg
      601 ggcgctttcc agctgtctca cgcacatcag gatgccgcgc atcaggtcca ccggttcaaa
      661 gcctgtcacg gcgataggcg tgcggtattg agccgcgatg ggttcatatt cccaataccc
      721 catcaccgcg cagacatgac cggcagcgag aaaggcgttc acgcggttat ccgggctttt
      781 caagatggca tgcatggctg gcggcaccaa aacgttcgcg ggcaggatgc tgaaattgtt
      841 gattcggttg cgctcagcgt ggaggatgct ggcggcgatg gacggaactg tggtctcaaa
      901 accaatcgcg aagaatacca cctgtttttg cggattttcc tgggccagtt tcaccgcgtc
      961 cagcggcgaa tagaccacgc gcacgtccgc gccttccgcg cgagcgctga agaggttggc
     1021 gtgcgcgccg ggtacgcgca gcatgtcacc aaatgaggtg aagacgacct cgggctggcg
     1081 ggcaatctgc agcgcgtttt caatcgcgtc gagcgaggtg acacacaccg ggcagccggg
     1141 gccgtgaatt aaagtgattt ccggaggtaa taactggtcc agtccgtact gtaaaatcgc
     1201 gtgggtctgc ccgccgcaaa tctccatgat gttccatgga cgcgtgacga gcctgcggat
     1261 ttctccggcg agctgccggg tgagcccggc gcttcggaat ccttcagtag cagccaggct
     1321 cattcctcgg ttttcccttt gctctcagcc tgattgaatt ctttaatttc tttgaggagg
     1381 cgcatggttt ccaacgcttc ttcttcagaa agcaagttca acgcgaagcc ggcatgcacg
     1441 atgacatact gcccgacctg cgcctcgggc acggtcgcca cgcaaacctc ctgctgtacg
     1501 ccgccgaagt cgaccatcgc catggcggtt ccgtccaagt catacaggcg ggtaatcatt
     1561 cccggtattc ccaaacacat catgcaatcc tcaattaact ttcacgcagc gcttgcaggg
     1621 ccaccatgac ctgcccgagc gacacacacg cgtcattggt tggcagggca tgatgataaa
     1681 gcgcaacgaa gccttccgct tccagcgcgg gcagcagcgc gcgcatcaat ctcatattct
     1741 gccacactcc gccggaaatt gccacgtgga aaattccagt ctcttcacgg ataagctggc
     1801 aggcttgcac ggacagcctg gtgatgctgt tgtggaagcg ggcggcaatc cgcgggacgc
     1861 ccaagccgcc ttgcaggtcc cggacgagct ccttcagcat ctccctgggg tcgatgatgc
     1921 cgtcctgcag ggtaaacgga taggcaccgg cttcgtccgg gtcagccgcg ctctccagag
     1981 cgatggctgc ctgggcttcg taactaattt cgtggcacaa gcccaaaatt gacgccgccg
     2041 cgtcgaagag cctgcccatg ctgctggtca ggggggcatt atagccgctt tccagctgcg
     2101 cccgcacgac cgagcgttct tctgtgctga gcgcctgaat tggtggcagc ttgtattctt
     2161 ccgcggagat gcccgcggcg gtcagccagc tcagcgccgt gcgggcgggt ttgcggactg
     2221 ccaggtcccc tccgggaagg cgtacgggca gcaggtggaa gcgccgttcg aaactggcgc
     2281 agccccccac caaaatctcc ccgccccaaa tgctgccgtc tgtgccgtag ccggtgccgt
     2341 cgaaaatcaa cccgatggca ggctcgcggc tttcccaggc gttttccgcc aggcaggcag
     2401 ccaggtgtgc gtggtggtgc tgaaccgcga tgagcggtaa gctttctgcc tggctgcggg
     2461 cccgcgcgta gcgggtggca agataatccg ggtgcaggtc gcacgccagc gcctcgggat
     2521 ggatgcggaa cagtttttca tagtgcccga ccgcctgttc gaacgattcg agcgtctcct
     2581 ggttttccag gtctccgatg aagtggctga caaaggcgta ttggtccctt gccaggcaga
     2641 aagtattctt gagctgcgtg cccgtgccta aaaccggctg cgctgccggc ggaatatgca
     2701 gctggccggg cgcgtagccg cgtgcgcggc gcgcgaagta aacctggccg cgcagcgccg
     2761 ttaccacaga gtcatccacg cgcatatgaa tcgggcggtt gtgcgtcagg aaggcatccg
     2821 cgaggggagc aagcctttgg tgggcttcct cgtcccggta tgccagcggt tcttcgctca
     2881 ggttcccgct ggtcatcacc agggcgtccg gaaagcccgg cgcgggttcc agcagcagca
     2941 ggtgcagcgg cgtatacggg agcataaagc ctaaccggac ctggtccggc gccgcgattt
     3001 tggcgagttc cttaccggca gccgtagttt cggacaggac aattggcgcc tgaggggagg
     3061 tcaacagcgc gcgttcctgg tcggagagtt ggacgtattt ttcaacaacc tcaagcgaaa
     3121 acgccatcag ggcaaacggt ttcccggtgc ggtgtttccg ttcgcgtaag gtttgcacag
     3181 ccagcgcatt gtgcgcgtcg catgccaggt ggtagccgcc cagcccctta accgccagga
     3241 ttttgccatc ctggagtgca gcgcgggcag cctggagggc ttgttcgccg ctttggatgc
     3301 gtgctccgtt ttcctcatac cagatttggg gtccgcatac cgggcaagcc accggttggg
     3361 cgtgaaagcg tctgtcggac gggttgtgat actccgccgc gcagtcgggg cagagcggaa
     3421 agccggccat gctggtgttg gcgcggtcgt aggggatttg gcgcacgatg ctgaagcgcg
     3481 gcccgcagtg agtacagttg ataaaggggt agcggtagcg gcggtctttg ggattgaaca
     3541 gctcggctct gcaatccgcg caaatggcga catccggcga aaccggcagg aagtcttccg
     3601 cccggttttc actttcccgg atggtaaatt ctgcgtacgc ttgcagcggg gcaggaaaga
     3661 cggcaaaact gtccaccttc gacagagcag gcggattgtt cctcaggctt tcggtgaagg
     3721 ctgcgcagtc ttcttcagag ccgtggattt caatttccac accccgggca gtgttgacca
     3781 cccagccact cagctggtgc tcctgcgcca gcaggtaaac tgtgggccga aaacccacgc
     3841 cctgcacgat gccggtgatg tggaaacgcc aattttgaag cttcacaggt ttacggtcag
     3901 gcctttggtg gcagcgtcct cgtcagccaa tccatccagg cctcaaagcc ttcgcctgtc
     3961 ttgcaagaca ccgggaaaat cttgagcccc gggttgagga tttcgacgcc ttgcgagaag
     4021 tattccatgc ggaagtctat gtaaggcagc aggtcggtct tgttgatgat gagcacgtcc
     4081 agaccgcggt agatattcgg gtatttgtag ggcttatcgt cgccctcggg gatggaggcg
     4141 atgacgatat tagcatgcgt tcccaggtca aagccggcag ggcaaatcag attgccgacg
     4201 ttttcgataa tgaccaggtt cagcgcttcc aggtccagtt tctgcaatgc caattccacc
     4261 atcacggcat ccaggtggca gtcgccgccg gtgttgattt gcacggctgg aatgcccaga
     4321 gcgctgattt tatccgagtc aatcgtaacg ggggctgtgt cgccctcaat cacgccgggg
     4381 cgaacgccgg ggggcagacg gcggagggtt tccatgatga agctggtttt ccccgcgccg
     4441 ggggaggcca tgacattcat tgcatacacc gaatgctcat ccagcagcgc gcggtttcct
     4501 gccgcgacac gctgattgga gtccattatc cgacggacaa tttctacgcg ttgggtcatt
     4561 attcctcttt caacacctca atatggtcaa caagcagctc ttcgccgctc ataatttcga
     4621 gcgcgatgct cccgcagttg gggcagggca tcagttcttc attaagttcg tattcatttc
     4681 cgcaagattt acagcgcagc cgggcaggca cgcgctgaaa atgcagtaca gctccggcac
     4741 tgggtgtgcc ctgactgata tggtcccagt agaattggac ggaatcgtct acgatggaag
     4801 acagctgccc gattgtcagg taaatatccg tcacccgact ggcgcaatta tccaacgcag
     4861 cccggttggc aatttcaaga atgctttccg tcacagcgag ctcgtgcata cgcgccatta
     4921 taacccaagg gcagccgctg ggggttcaga ctctcaggct gtaagcgcaa aggaatcata
     4981 tcctgaaata gcaaaaaaga cacgtcctca ttaagatcag gagctgtgaa atcattattc
     5041 ccttactcct atttttttca aaggaatgat gctgccctga cgcataagga tttagcagcc
     5101 ttgccctctt ccttcccttt caagcggacg aaaacaaagc gaatatcctg atgaacgagc
     5161 gcagggaagg ggagctgatc tttgaacgaa ggcacagccc tctatcaaac tttttccttt
     5221 ccccagcggc ttgagcgagg caaaatagag ccgctttgcg ttgggggagg ggtcagggtt
     5281 agggcaagcg ggaaacagga tcgaaaagaa gaaataagaa tgttcgtttc gaggaaaaac
     5341 ccgtcctggc gatttcatcc actccaggac aattctactt agccttggca ttcagacact
     5401 caggctcaag gtcttcacag gctcaggcgc tccggatggc tgtacacgtc gaactgggcg
     5461 ctgcgcaggt agccaattaa ggtgatgccg gcttcccagg cggtctcaac tgccagagct
     5521 gtgggggatg tgcgcgagac aatcacgggc acaccaagcg cgaaagtctt gaacaccatc
     5581 tccgagctga ttctgccgct gagcagcagc agattcggcg tgaagggtct gttttgcagc
     5641 aggaacaagc cggtgagctt atccacgcag ttatgccggc ccagatcttc caccatgata
     5701 ggcacgcgca agccgtcgct cagacctgca ctgtgaaaac cgcccacctg gtcgtgcagt
     5761 tcctgctggt ccatgaattg gaggtacaga gccagcacat cttcgacagt gatccggaaa
     5821 tccgtgggca aatgcggcag cctcaagctg ctttccagcc catagccagt ggaggtgcgg
     5881 tggaaatgag ttggtttttc cgctgtgcgg ctcagtttga ccataatcct gctgccgccg
     5941 tcgccaacct ggatggagct gacctcccca aggtccttaa tgacattctc gccatacagg
     6001 aaaccaaggg cgagagcgtc caggtcagta ggggagcaaa tgaaagtcag ccagggctcg
     6061 tcgttgacat acagcgcggc tgcgtgttcg ctgacgatcg gtttgcgcgc cggcagaagt
     6121 ttgccgctgg cacgcgagaa ttgccagaat tctttttcct gatagggttc agtcattcgg
     6181 agggcttcct ttctggatgt tgaggactaa aaccatcccc acgcaggaac gcgataagca
     6241 tctgcgcggc agaaaacgca agttctttgg ttggcgcaga gagctcttgg gaaaactcaa
     6301 aggcatttcc ccgcacggaa agcagccagc attccgggca ggcgctaaat agagccgtgg
     6361 tgatggacag cagttcgccg acggacatgt gatgcgtgaa agcggagtgc tcccacgctg
     6421 gtccagtcct ttcaaagacc aggtccggca gttgggggga attatgcgcg tcgatgaaca
     6481 ccacccgctc gtacgcagtc aagtcttctg atatctcagg cagtaattgg tagagatacc
     6541 acagccgcag cgcgccctcg gcggcatcca cccaatcccc agggagttca ggcgccgcgg
     6601 cgccaaattc gcgcgcgata tccgtcagga tatgccagcc ggcgccgtca tcctgcctgt
     6661 cgcggttacc gtaggcaatg agcaaggttt ttttgggtgg gttcataggc agaaaaaact
     6721 cccctcctat gataccaaag aaggggagct ttgtatctga atgaaagatt agcgccggat
     6781 tacgcgcaca agctcgccct gcttattaaa gacgctgacc tccagcggca tgctgccaac
     6841 ggcgtgcgtg gagcaggaaa ggcaggggtc gtgcgcgcgg atagcgtgtt caacccggtt
     6901 cagcatacct tccgtgatat cggggccgtg cacgtatcgc ttggcgactt cgtccaccgc
     6961 gcggctcatg gcgtagttgt tattgccggt ggcgacaatc aggttgacct tggtcagctg
     7021 tccgctgccg ttgctccagt agtgatggaa cagggttcca cgcggcgctt cgataatccc
     7081 gacgccctgc tcctgcggct gctggcgggt gttcaggatg tcggagctga gaatatccgg
     7141 atcctcaagc aaaatgcgcg cgtgttctgc ggcaaacagg gtctcaatca ggcgcgcgta
     7201 gtgataatgc aaggtgttct caaccggttt gccgccattt aaggccttga aatgcttgaa
     7261 ttcttcgttc gcgagcggcg tgtcaatctg gtcagaattg ttcaggcgac ccagggggcc
     7321 gacgcggtag acgccttccg ggtagccaag tttcttgtag tacgggaact tcagataaga
     7381 ccaggattcc acatgttcgg cgatgtaatc cagataatcc tgcgggtcaa acttttcgag
     7441 ctctttgccg tcccgtccgg tcaggcggat gtctccgtcg tagagttcca ggctgttgcg
     7501 aggggtcaca agccccagat agccggtcgg gaagaccgcg aattcgtcga tttcggtctg
     7561 atgtgcttcg atccaggcat ggataatctg gatgccgatt ttgatggtct caatttgctc
     7621 gtccacctgc gccagcaggc ggtcgcgccc ggcttgtgtg aattgacggt tgacgccgcc
     7681 ggggatagca aaattcgggt ggacgctgcg ttcgcccagc tctgagatga ttgcctggcc
     7741 gaacttgcgc aggcgcacag ccctgagcgt cagctgagga tccgcctgga tgagaccaac
     7801 gacattgcgg gtggcggggt catggtcaaa gcccaggata aggtcaggac cagccaattc
     7861 aaaaaagtgc atcccgtggc tctggataat ctgacccatg tgcatgagtt cacgcaggag
     7921 ggaggcgggc cggggaggcg ggcagccaag cacgacatcg ccggctttgg cagccgcgag
     7981 gtggtgtgat accgggcaaa tgccgcaaat gcgcggggta atttgctgca tttccgcaac
     8041 caggcgtcct tcacaaaatt tctcgaaacc gcgaaattcg ttcacgtgga aataggcatg
     8101 ttccaccgag ccatcatcat tcatgtgaat ggtgaccttg gcgtgacctt cgatgcgggt
     8161 tacaggttca atcgtgattt tttgggcaac catgcggcct cctaatgcca atcgttaaac
     8221 tcaccactga cttcgggcat cctgccctgg gcgagctctg agagaatgta gaaaattgca
     8281 tcagcgctgg gagcgcagcc aggcaggaag aggtccactt tgaccacatc gcttaccgga
     8341 cggacgtgca ccggcgtgga gagttccggg tcatccggga ttttgccgct ctcgtccgtg
     8401 ctttcggttt cgacgtacgc gcgtttcagg gctgcttccg cgccttcaaa gttgcgcatg
     8461 gcgggcactc cgccgaatac cgcgcaatcc ccaagggcga caaggatctt acagcgggcg
     8521 cgcatcttct tcgcgacttc ttcgttgtaa gtgttactga tgccgccttc cagaatgcct
     8581 acgtccacgc ctgattcatc gggttccttc aggtcggtga ttggtgtcgc gcgcaggtca
     8641 accagttcgg taagggctac gatgcgttcg tcaatgtcaa gaaacgacat atggcaaccg
     8701 gcacatcctg ccagccagtc tgatgctact ttgggtttag ccattttcaa tacctctcaa
     8761 ttattccagt ggtcatcatc taaaaatcca ggtcaaccgg gctgggctgc tgtttgaggg
     8821 cagttttcca atcctgcccg ccgcgggcgt tcagcgcctg cacgagcgca tctgcgacag
     8881 ccgcagaggt cttcacggat tcaggagcgt cgagcgcggc gagtttctgc tgcaggcggc
     8941 cttccgtgct gaggtagctg ccgccttctt ccgcccaggc ggcacaggga agcactaccg
     9001 cggctctttc ggtcagcgcg gaggggtgtg cggcagaaac cacaaggagc ggaatgccgg
     9061 cgcagtgttt caggagtgct tcggtcggtt ctgcatcacc cagggcaaca aaagccgctt
     9121 tcactttctt tccatccgcg gctgtggtgt agcccagttg ggccgcagcc aggctgttcg
     9181 cctgttcttt gatgatcaga accgggaagt tgttctgccg gcagaacagc accaaacgca
     9241 ccagcgcgtc gtaatgttcc aggctggcaa attcaccacc cagcaccacc acaggatgct
     9301 gtgcgtcttt gagcatggta acagcgcgca ggatttcagc gctcttgatg ccggtctttt
     9361 cttccaggtg cgcgatgttt tcctccgcga atttatcgcc ctcgtgcaga agcgagtgcc
     9421 aggcagaaac aagcccgtta acgaggtcaa aggagctgcc ttttttctgc tggattctca
     9481 gtgcagaacg ggcgataagc ggcttttgcc gggttgtaat ggagataatc ctgctgtttt
     9541 ccagtacctg ccgcttgagg aagaagcctg ccacctggtt gtcgttttcc aggtcgctgc
     9601 caatcaacag agccacgtcc acatgcttca gcgcctccag tttggcttcg aagggaccgt
     9661 ggagttcctc cgccagtttc acgcttgcaa gggcggcttc gttgtcatcg ctgaggagca
     9721 ggtttgcgct gcgcagcccg ccggtgaagg cggatttcag ggcagccagg ctttcaatcg
     9781 gaagtttcgc cgaggcaaat aacgctgctt catccgcggc agcccgcagg tgctgcgctg
     9841 cttcagtgag ggctgcctcc caggtgacgg caacgcgctt accatcccgc aggatttccg
     9901 gaacggataa cctttccgct ttggattcaa ggggcatgta gcgacctttt tcacacagca
     9961 gcccttcgcc tggttcggag ttccataccc cgtcaatccg caccaggcgg ttattccggg
    10021 tgaggacatt gcgctggcag ccaatactgc actccgaaca aacagagacg accggtgtga
    10081 ggtcgctctc gcggccctgg taggcgctgc ggcggtccat gagcgcgccg gtggggcaaa
    10141 tctggacgca ggcgccgcag gagatacagg tgctctcccc caggggtacg ccgtaatcgg
    10201 caaccaggaa gctgttggcg ccgcgttctt caaagccgag cgtaaaattc ccgaccaggt
    10261 cgccgcaggc gcgcacacag cgccggcaaa ggatgcagcg gttgttatcc acgaagatat
    10321 acttgtgcga ggcatccacg gcgtagggcg agtagtttgg cgtcagcggc caatgggtga
    10381 ggctttgttg gtaagcggca cattgcagtt cgcagtctcc atcggtcgcc tggcagtaca
    10441 tgcagaaatg gttgcgttcg ctgaagagca tggagagcac gaatttacgg gcttcctgca
    10501 ctttaggcgt gttcgtgcgc acaaccatgt tgttgctggt tggcagggtg cagcttggct
    10561 gcagaatgcg cgcgccttcc acttccacca tgcacaaacg gcagccgccg taaggcttga
    10621 ggctgggatg gtggcataaa cggggtactt tgatgccaac tttctctgcc gcttcgagga
    10681 ctgtcgtccc gtctggaact tctacctgaa tgccgtctat cgtgagtgtt accataaact
    10741 tgaactccat ctcttgcaat gataatttcc cgaacaggtc agctcataga gcagacaccc
    10801 gccggacaga ccttaagtcg gatatgcgct tccacctcat ccctgaagtg gttaagagtg
    10861 tcccgcagcg gaacggcagc agtctgaccg agcccgcagg tggaaagttc gttcagttgg
    10921 acgctaatct cgcgcaaggc atccaagtct tgcaggctgc cgtgccccgt ggagatgttc
    10981 gtcagaatct cataggcttt ctgcgtgcag atgcggcagg gcgagcactt gccgcaggat
    11041 tccttgcgga aaaagttcag caggacccgc gccaggtcga ccacgcatac gctgtcgtta
    11101 cagatgagca gcgcgccgga gccgagcacg acgccggctt tggcgaagga agcgaagtcc
    11161 atcggcgtat cttgcaggct ggcagggatg atggatccgc cggagccgcc agtctgggct
    11221 aacttaaagt gcccatcctt gatgcctttg ccgtaaatcg taatcatttc ccgcagggtg
    11281 atgcccatcg gcgcttcgat cagaccggtg acattcaggt tgcccaaaag cgtgtaaact
    11341 ttggtgcccg ggctggaagg cgtgccgaat ttgcggtacc aggccgcgcc gttgcggagg
    11401 atggacggaa cattggccag ggtctccacg ttgttgacca ggctgggttt gccccacaaa
    11461 ccttcggtcg tcgggtaggg cggcctggag cgcggctcac cgcgtttgcc ttcaatggac
    11521 tcaattaagg cggtttcttc gccgcagata tatgccccgg cgccggtgtg caggtgcaga
    11581 ttgaacgaga gcgaggaacc cagaatgaaa ttgcccaata gaccggagct gcgcgcctgg
    11641 gcgatagcgg tttccatgcg ctggatagcc aattggtatt cgccgcgcag ataaacataa
    11701 ccttcggttg cccccaccgc gtacgcggca atcagcatgg cttcgataat ctggtgcggg
    11761 tcgccttcga tgagcacgcg gtccttgaat gtgcccggct cgctttcatc cgcgttgcag
    11821 atgacatact ttttgtccgc gcgcgcctgg cgcacaaaac cccacttaag accggtcggg
    11881 aagcccgcgc cgccgcggcc ttgcagaccg gactccttga taagggcgat gacctgttcg
    11941 ggggtcatgc tcagggcatt gcccagaccc tggtaaccgt cattggcgat gtaatcctca
    12001 atgctgttcg ggtcgataat gccgacattc ttgagaacga tgcgtgtctc ggcgggaagc
    12061 gtccctttcc gggcagcaac ccaggcaatc c
//
LOCUS       DAOQAD010000055        12046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_5957_length_12046_cov_6.547411, whole genome shotgun sequence.
ACCESSION   DAOQAD010000055 DAOQAD010000000
VERSION     DAOQAD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12046
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5957_length_12046_cov_6.547411"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PKL60_05695"
     CDS             <1..400
                     /locus_tag="PKL60_05695"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNW95661.1"
                     /translation="PGSPTQAAQPTAASGTKCYQMDFLGDVNYPPDTAVKAGEKFIKK
                     WKIKNTGTCTWSTDFDVVLVWGDKIGKNGDVTYAIKPGDTYTVSVEITAPATAGTYIG
                     YWMMTDNQGARFGYGPGGDYSLAVKIQVVK"
     gene            926..2170
                     /locus_tag="PKL60_05700"
     CDS             926..2170
                     /locus_tag="PKL60_05700"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNW95662.1"
                     /translation="MKTKKNSLGFWAVALMLVSALALSACNAPFAPTQDLDDAVQRAM
                     QTLQAQATLEAFQTLVAQMTPTAPATAAAPTQGVPGQTQALPTNTSVPPTSVPPTVVI
                     TPIPPTATRIPPTPTRIPPTPTPKPCNRIDFRGDVTIPDGAKIIGGTSFVKTWRLKND
                     GSCTWTTAYDLAFVDGDRMGAPSYVDMPKQVKPGESIDLSVSMIAPGTPGVYTAHFML
                     VDQNGNRFGPKSDGKGTFWAKIESIRGQGVVFNFAAEACRATWTSNNSSEALKCPGKT
                     SDISSGYVIAQAEPIREDGGKENEPGLITRPDNTASGFIQGRFPEIAIQNGDKFRATI
                     QCEGGMEDCDIYFTLQAQAGSEPLMELGTWHEVFDGHWNPVTVDLSSFAGRNVTLYLI
                     VRNGSTAADNSGLWLNPIIYRP"
     gene            2315..3076
                     /gene="tpiA"
                     /locus_tag="PKL60_05705"
     CDS             2315..3076
                     /gene="tpiA"
                     /locus_tag="PKL60_05705"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNW95663.1"
                     /translation="MKSRKPMVAGNWKMNKTLPESRDLIRAILPLVEGVDGVDIVVCP
                     PFVSLGVSKRETEGTAIKVGAQNMHWEASGAYTGEVAAGMLVDLCQYVIIGHSERRAM
                     FGETDATVNKKVHAALKAGLKPIMCVGETLTENQAGQTAAVVEGMVRGGLQDVTPEQA
                     QNIIIAYEPVWAIGTGLAATPEGANSVHKDVIRPVLRELFGAEVGDALRILYGGSVTD
                     KNAAELFSQSDIDGGLVGGASLKPDPFAAIVKAAL"
     misc_binding    3195..3416
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3467..4498
                     /gene="pheS"
                     /locus_tag="PKL60_05710"
     CDS             3467..4498
                     /gene="pheS"
                     /locus_tag="PKL60_05710"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNW95664.1"
                     /translation="MPVLEQLEHEKTTALSELESARSEAELEAWRLAHLGKSSPVMLA
                     FSALGSASKEERPLIGKSANAVKLALESAYAAKKEALRQLALTSSLEAERLDVTLPGR
                     ALRRGRLHPLNQTLREICRVFGDMGFQVYRSPEVETDDYNFTFLNMPPYHPARDMWDT
                     FYTEKEGVLLRTHTSPGQIHIMRERAPEAIRAILPGTTMRYEQLTARSEIEFVQVELL
                     VVGKGISFADMKGTLNDFAARTFGKDARTRLRPSHFPFTEPSAEMDVECFVCGGKGCG
                     VCKGSGWLEILGCGMVHPTVLEYGGYDPHVFSGFAAGMGIDRSTLMRYRVDDIRHFRN
                     NDVRFLEQF"
     gene            4518..7055
                     /gene="pheT"
                     /locus_tag="PKL60_05715"
     CDS             4518..7055
                     /gene="pheT"
                     /locus_tag="PKL60_05715"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNW95665.1"
                     /translation="MKAPLSWIKDYVNLDGLSIEEIARRLTMTGLEVDGILLVGLPKP
                     EGEKHEFKYEGLSWDPQKFVVARVDEVMPHPNADKLVLCRLNDGASEMIILTGAPNLY
                     PYKGIGPLAEPLKVAYAREGAQLYDGHQPGQVLTRLKRAVIRGIESFSMICSEKELGI
                     SDEHDGVIILDADAPTGMPLADYMGDAVFDISILPNMVRDASIIGIARELAAVTKREL
                     REPQGLKLGRGTRLGELVSLEVRDPELNPRFMLGMIEDANPKPSPYWVQRRLSLAGMR
                     PIDALVDATNYTMLDTGEPLHAFDYDLLVQRAGGSKPAVITRTAAPGEKLTTLDGNTL
                     TLDESMELVCDSAGPLSLAGVMGGSETGIHAGSSRVLLEAASWNFINIRKTISKVRIA
                     SEAGWRFSRGVHPALTEYALSLCLRRMAEWAGGKPVDGVLDAYTRLQVDPLVCLSEGE
                     IVSALGASIPLEEAADILTRLGFGCRVEEGQLLAQTPATRLDIGEGIIGKANLIEEIS
                     RVYGYDRLPAARLSSELPAQVGNPRLDMEERMRDILVSAGLQDTVAYRQTSPEREARL
                     RPENTPDPTLEYVRIKNPITPDRTVLRRSSLATMLELLEHNHKFRPGLAMFELGPVFL
                     PVEGQLLPHEALRLTIGMTGAREYPTWQTKKPEQMDFFDLKGVIEALLKGLHVANISF
                     RPAQHPSYHPGKCAEVWAGEQKLGVLGELHPLVREHYEFDAGAVLAAEFDADLLVDLS
                     QTLFDHAPISNYPSMLEDIALIVPEETPAAALEASILRAGGKLLVNARLFDIYRGEKL
                     GEGKKSMAYQLTYQAADRTLTDKDAETIRNRIVRALAREFGAVLRSQ"
     assembly_gap    7397..7496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7581..8384)
                     /locus_tag="PKL60_05720"
     CDS             complement(7581..8384)
                     /locus_tag="PKL60_05720"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNW95666.1"
                     /translation="MISQQPFHTVYFSHGGGPLPILGDPGHFAMVRFMSALAPRLIKP
                     EAILVISAHWEEKQPTLLGAENPPMFYDYYGFPEEAYRIRYPAPGSPELAARVREVLA
                     EASIPAAINPTRGFDHGLFIPLSLMYPQADIPAIQLSLVRGLDPEQHLRLGEALRGLD
                     DGKVLFIGSGFSFHNLQAFSWNPGTAPDPANDAFQEWLIETCSRQADYAVTRAALRNW
                     ESAPYARYCHPREEHLMPLLVCAGLAGKSGEVIFDDLILGKRGIALGWE"
     gene            8616..8969
                     /locus_tag="PKL60_05725"
     CDS             8616..8969
                     /locus_tag="PKL60_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNW95667.1"
                     /translation="MNGTFKVIFHLDDYDSDRIDMTFANIANLFDDMQDLDLEVELLV
                     NGPAVQAFTNDNTHVLERVQQLAALGVETLLCRNALRVHSILPKDLPGEVKVVPSGVG
                     WLVRRQAEDWSYIRP"
     gene            9255..11597
                     /locus_tag="PKL60_05730"
     CDS             9255..11597
                     /locus_tag="PKL60_05730"
                     /EC_number="7.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasma-membrane proton-efflux P-type ATPase"
                     /protein_id="HNW95668.1"
                     /translation="MTASEVESTNPSTGLTAAEAAERLIKFGLNTISEAKQNQLLMFL
                     KKFWAPVPWMLEITIVLELYLGKHTEAIVIGALLVFNAILSFAQESRAHNALSLLRQK
                     LSVQVTTRRDGQWRRIQAENLVPGDIVHLRMGDIIPGDAILIDGLVEIDQSALTGESL
                     PVECGAGQAVYAGAVIKRGETTCEITSTGASTKFGKTAELVREAKTVSHLQDIIFTIV
                     KYLVIADVILVALVIAYALYAHIALQMVLPFALIILVASVPVALPATFTLATALGAAN
                     LAKQGVLVARLSAIEEAAAMTILASDKTGTITENRLKLSAVHAYPPFHVEDVIRFGAQ
                     ASRAATQDPLDLAIIEGAHDRQIGLVGERLDYTPFDPATKRSEALIAKPDGNTLRILK
                     GAPLTIAALAGGSDINADVAKLALSGNRVLAVAAGPDTGPFQLVGLLGLQDTPRPDSK
                     SLIHELNNLGVRVVMVTGDDALTAKAVATQVGIPGEPCPVEKLLGIHQEGAPDCNIFA
                     GVFPEDKFRLVQGLQKSGQIVGMTGDGVNDAPALKQAEVGIAVASATDVAKAAASIVL
                     TKDGLSNILDAIEASRRIYQRMLTYTLNKIIKTFQISLFLSLGFVLAREFVVTPILII
                     LLLFANDFVTMSLATDNVNYSLKPDRWKILPLVAASFGLALPVLLLSFGFFYVANYVL
                     RLPLNQLQTLMFVMLVFTGQANVYLVRERHHFWNSRPSRWMLLGTLADIILVLIFASQ
                     GILMAAIPLSYVVISLLVVAAYIPCADWIKLLVFKLTKID"
BASE COUNT         2625 a         3517 c         3340 g         2464 t
ORIGIN      
        1 cccgggctcc ccaacccagg ctgcccaacc caccgccgct tcgggcacca agtgttacca
       61 gatggatttt ctgggcgacg tcaactatcc gcctgacaca gctgtcaaag ctggcgaaaa
      121 atttatcaaa aagtggaaaa ttaagaacac cgggacctgc acctggtcta ctgattttga
      181 cgttgtgctc gtctggggcg ataaaatcgg caagaacggc gatgtaacct atgccatcaa
      241 acccggagat acgtataccg tgtccgtcga aattaccgcg cccgccactg ccggcaccta
      301 catcggttat tggatgatga ccgacaatca gggcgcccgc tttgggtacg gtcccggcgg
      361 ggattactcg ctggcagtta agatccaggt ggtcaagtaa acctgctttt gttgtgtacc
      421 attatcaagc cgcccatcag gcggcttttt tttatctttt ctcaggcagc gtcccaggaa
      481 atgcacgatc gacgggagcg cagtttgtgg gttggattgc gcttctccgt cgctgcaccg
      541 aacctaccgc tgaaatctgt cattccgaac gaagcgaaga atctgttggt tgaaaaacaa
      601 gatccttcgc tggctctcag gatgacaggc agatcgcttc gctgcgcacg cgctgacgaa
      661 cggaaagaca ggtgaatgcc gcacctgctc gcgctgaatt ggaatctgtc attctgaacg
      721 atggcaagaa tctgttggtt gaaaaacgag atccttcgct ggctctcagg atgacaggca
      781 gatcgcttcg ctgcgctcgc gctgacgaac ggaagcgccg tccaaatcaa tccaaaccaa
      841 tttgcgccag gctgcggaac aaaacatcgg cccagttcgt cataaaatag gtgtatgatg
      901 ttattaagct ttaaaggagc gtaagatgaa aacgaaaaag aactcactgg gattttgggc
      961 agtcgctctg atgcttgtca gcgctctggc gctctccgcc tgcaacgcgc ctttcgcccc
     1021 gacccaggac ctcgacgacg cggttcagcg cgctatgcaa accctgcaag ctcaagctac
     1081 cctggaagcc tttcagaccc tggtcgccca gatgacccca accgcgccag ccaccgcagc
     1141 ggctcccacg caaggcgttc ccggtcagac ccaggcgctc ccaacgaaca cctccgtgcc
     1201 cccgacttcc gtccctccca cggttgtgat tacccctata ccgccaacgg ctacgcgcat
     1261 tccccccacc cctacccgca tccctcccac ccctaccccc aagccctgca accgcatcga
     1321 cttccgcggt gatgtgacca tccccgacgg agcgaagatc atcggcggga ccagcttcgt
     1381 caaaacctgg cgcctgaaga acgacggctc ctgcacctgg accacagcct acgacctggc
     1441 tttcgtcgac ggcgaccgca tgggcgctcc atcctatgtg gacatgccca agcaggtgaa
     1501 acccggcgaa tccatcgacc tgagcgtctc gatgattgcc cccggaacac ccggcgtata
     1561 cactgcccac tttatgctcg tagaccagaa cggcaaccgc tttggcccca aatcggacgg
     1621 taaggggact ttctgggcta agattgaatc catccgcgga caaggcgttg tgttcaactt
     1681 tgccgcggaa gcctgccgcg ccacctggac ctccaacaat agtagtgaag ccttgaaatg
     1741 ccccggcaag accagcgata tcagctctgg ttacgtcatt gcccaggccg aacctatccg
     1801 cgaggacggc ggcaaggaaa acgaacccgg actgattacc cgcccggata acaccgccag
     1861 cggctttatc cagggtcgct tcccggaaat cgcgattcag aatggagata agttcagagc
     1921 caccattcaa tgcgaaggcg gcatggaaga ttgcgacatt tactttaccc tccaggccca
     1981 ggcaggcagc gaacccttga tggaactcgg cacctggcat gaagtctttg acgggcattg
     2041 gaacccggtt acggtggacc tgtcctcctt cgccggccga aacgtcacgc tctacctgat
     2101 tgtgcgcaac ggctccacgg ccgcggataa cagcggtctg tggctgaacc ccatcattta
     2161 caggccgtaa cgcgcggcga aaaacaagca acacaggctg cccggctggg cagcctgttc
     2221 ttttttccag gctctacaag gagaaaaccg gcttctggca agctttgcgt ggtaaaatca
     2281 aaccaatcaa tttcccctca agaaaggaca ccccatgaaa tcaagaaaac ctatggttgc
     2341 cggtaattgg aagatgaaca agaccctacc tgaatcgcgc gacctcatcc gcgccatctt
     2401 gcccttagtg gaaggggtcg acggggttga tattgtcgtt tgcccgccct ttgtaagcct
     2461 tggggtttcc aagcgtgaaa cggaaggcac tgcaatcaag gttggcgctc agaatatgca
     2521 ctgggaagcc agcggagcct acaccggcga agtcgcggct ggcatgttgg tggatttgtg
     2581 ccagtacgtc atcatcgggc actccgagcg ccgggctatg ttcggcgaaa cggacgcgac
     2641 cgtgaacaag aaggtccatg ccgcgctgaa agccgggttg aagccgatta tgtgcgtggg
     2701 tgaaaccctg accgagaatc aggctggcca aaccgccgcg gtagtggaag gcatggtgcg
     2761 cggcgggctg caagatgtga cgcccgaaca agcccaaaac atcatcatcg cctacgaacc
     2821 tgtctgggcc attggcaccg gcctggctgc caccccggaa ggcgcgaaca gcgtgcataa
     2881 ggacgtcatc cgcccggtcc tgcgcgagct tttcggcgcc gaggtcgggg acgcgctgcg
     2941 catcctctac ggcggttcgg tcacggataa gaacgccgcg gaactcttct cccaatccga
     3001 tatcgacggc ggcttggtcg gcggcgcttc gctcaaaccc gatccctttg ccgcgattgt
     3061 aaaagccgcg ctctgatatt gcgcttcgaa cctcttcggg cagcctttgg gctgcccgat
     3121 tactacttcc gaccttccca ttccccttaa gaaaccgcca acttgacaag cctctccgaa
     3181 aacttataat taccctatca actgtgaacg aggaaagtag cccatccaga tccgacaggg
     3241 acgtggcgcc acagactgga agcggtaccc ggcaaaaggt gggggaagtt ccctcgggag
     3301 ttgtgcgggt gaactgccag tagtccgcac cgggctttcc cgttacagaa accttgagcg
     3361 ggctgcctcg agcagccaac aagggtggta ccgcggcaga tttgcccgtc ccttgaccgg
     3421 gcgggctttt ttattgcctg ccgcactatt tttatcgagg tgaaaaatgc ctgtgctcga
     3481 acaattggaa cacgaaaaaa ccacagccct ttccgaactg gaaagcgccc gcagcgaagc
     3541 cgagctggaa gcctggcgcc tggcgcacct gggtaagtcc tccccggtaa tgctggcctt
     3601 ttccgcgctg gggagcgcgt ccaaggagga gcgcccgctc atcgggaaaa gcgctaacgc
     3661 agtcaagctc gctctggaga gcgcctacgc cgctaaaaaa gaagccctgc gccagctcgc
     3721 cctcacaagc tcgttggaag cagagcgcct ggacgttacg ctgcccggcc gcgcgctgcg
     3781 ccggggcagg ctgcacccgc tcaaccagac cctgcgggag atttgccgcg tgttcgggga
     3841 tatgggcttt caggtctacc gttcgccgga agtggaaacg gacgactaca atttcacttt
     3901 cctgaacatg cccccgtacc atcccgcccg ggacatgtgg gatacgtttt acacggaaaa
     3961 ggaaggcgtt ctcctgcgca cacacacttc cccgggtcag atccacatca tgcgcgaacg
     4021 cgccccggaa gccatccgcg caattctgcc cggcacgacc atgcgttacg agcagctcac
     4081 cgcgcgctct gaaattgagt tcgtgcaggt agagctgctg gtagttggca agggcattag
     4141 cttcgcggat atgaaaggca ccctgaacga ttttgccgcg cgcaccttcg gtaaggacgc
     4201 ccgcacgcgc ctgcgcccct cgcacttccc tttcaccgag ccaagcgccg aaatggatgt
     4261 ggaatgcttt gtgtgcggcg gcaagggctg cggagtatgc aagggcagcg gctggctgga
     4321 aatcctgggc tgcggcatgg tgcaccccac cgtgctggaa tacggcggct atgacccgca
     4381 tgtcttctcc ggcttcgccg ccggtatggg catcgaccgg tctacgctga tgcgctaccg
     4441 cgtggatgat atccgccact tccgcaacaa cgatgtgcgc ttcctggaac agttctaagc
     4501 ccggaggagg aggaaaaatg aaagcaccct taagttggat taaagattac gtcaacctgg
     4561 acgggctcag catcgaagag attgcccgcc gcctcaccat gaccggcctg gaagtggacg
     4621 gcattctgtt agtcggtctg ccaaaaccgg aaggcgaaaa acacgaattc aagtacgagg
     4681 gcctctcctg ggacccgcag aagttcgtgg tcgcgcgtgt ggacgaggtg atgccgcacc
     4741 ccaacgcgga caagctggtc ttatgccgcc tgaacgacgg cgccagcgaa atgatcatcc
     4801 tgaccggcgc tcccaacctc tatccgtaca aaggcatcgg tccgctggcg gaacccctga
     4861 aagtcgctta cgcgcgcgaa ggcgcccaac tctacgacgg gcatcagccc gggcaggtgc
     4921 tcaccagatt gaagcgcgca gtcattcgcg ggattgagtc cttctccatg atttgctcgg
     4981 aaaaagaact gggcatctcg gacgaacacg acggcgtcat cattctggat gctgacgcgc
     5041 cgacaggcat gcccctggcg gactacatgg gcgacgcggt cttcgacata tccatcctgc
     5101 ccaatatggt gcgggatgcg tccatcatcg gcattgcgcg cgaactggcc gcggtgacca
     5161 aacgcgaact gcgcgaaccg caaggtttga agcttggccg cggtacccgc ctgggcgagc
     5221 ttgtcagcct ggaagtgcgc gaccccgagc ttaatccgcg ttttatgctt ggaatgattg
     5281 aagacgccaa ccccaaaccc agtccatact gggtccagcg ccgcttaagc ctggcgggca
     5341 tgcgcccgat agacgcgttg gtggacgcca ccaactacac tatgttggac acgggcgaac
     5401 ccctgcacgc ttttgactac gacctgctcg ttcaacgcgc cggcggcagc aaacccgccg
     5461 tgatcacgcg caccgctgcc ccgggcgaga agctgaccac cttggacggc aatacgctca
     5521 cactggacga gagcatggaa ctggtatgcg acagcgctgg tccgctttct ttggcaggcg
     5581 tgatgggcgg cagcgaaacg ggcatccacg ccggcagttc gcgcgtcctg ctggaagctg
     5641 cctcctggaa tttcatcaac atccgcaaga cgatcagcaa ggtgcggatt gccagcgaag
     5701 ccggctggcg cttcagccgc ggagttcacc ccgctctgac ggaatacgcc ctgagcctgt
     5761 gcctgaggcg catggccgag tgggctggtg ggaaacccgt ggacggcgtt ttggacgcct
     5821 acacccgctt gcaggtggac ccgctggtgt gcttgagcga aggggaaatc gtttccgcgc
     5881 tcggcgcctc cattccgctg gaagaagccg cggatatcct cacccgcctt ggctttggct
     5941 gccgggttga ggagggacag ctgctcgcgc aaacccctgc cacgcgcctg gatatcggcg
     6001 aaggcattat tggcaaagcc aacctcatcg aggaaatcag ccgcgtttac ggctacgacc
     6061 gcctgcccgc tgcccgtctt tcctccgagc tgcccgccca ggtgggcaac ccccgcctgg
     6121 acatggaaga gcgcatgcgg gacatcctcg tgagcgccgg cctgcaagat acagtcgcct
     6181 acaggcagac ctcgcccgaa cgcgaagcgc gcctgaggcc agaaaacacg cccgacccca
     6241 cgctggagta cgtgcgcatc aagaacccca tcactcccga ccgcaccgtg ctgcgccgca
     6301 gcagcctggc gaccatgctg gagctgttgg aacacaacca caaattccgc cccgggctgg
     6361 cgatgttcga actggggccg gttttcctgc ccgttgaagg tcagctcttg ccgcacgaag
     6421 ccctgcgcct gaccatcggc atgaccggcg cgcgcgaata tcctacctgg cagacgaaaa
     6481 agcctgagca aatggatttc tttgacctca aaggtgtaat agaagccctc ctgaagggtt
     6541 tgcacgtagc gaacattagc ttccggccgg cgcagcaccc cagctaccac ccgggaaaat
     6601 gcgcggaagt gtgggcgggc gaacagaaac tgggcgtgct gggcgagctg catccgctcg
     6661 tacgcgagca ttacgagttc gacgccggcg cggtgctggc cgccgaattc gacgccgatc
     6721 tgctcgtcga cctcagccaa acattgttcg accatgcgcc tatctccaac tacccatcca
     6781 tgctggaaga catcgcgctg atcgttccgg aagaaacccc cgcggcagcc ctggaagctt
     6841 cgatcctgcg ggcaggcggg aaactgctgg taaacgcgcg tttatttgat atctaccgcg
     6901 gcgaaaagct gggcgagggc aaaaagagca tggcttatca gctcacctac caggcggcgg
     6961 accgcacgct caccgacaag gacgctgaga caatccgcaa tagaatcgtg cgggcgctgg
     7021 ccagggaatt cggcgcggtg ctgcgcagcc agtaggccgg agggcggggg ccagcattgg
     7081 gatatgccgc ttcggcatta cgacatctcg attcgtcatt gcgatattcc gattcgtcat
     7141 tgtgaaccgt tttgccgtga tttagtgagc ctttatatta ctgacaaaca ggcaaaacgg
     7201 tgtggcaatc tgcctgaatg ccagtcgata agccaacggg gagatcgcca cgctgacgct
     7261 cgcgatgacg aacaggaata gatgcttaca aagttcactt cgtgccgctg cgctccagga
     7321 cgacgcctga ttcgtcattg cgaaccgttt tgccgtgatt tagtgagcct ttatattact
     7381 gacaaacagg caaaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaac
     7501 aggaatagat gcttacaaag ttcacttcgt gccgctgcgc tccaggacga caaacaaaac
     7561 ccttattcgt cccgaaaacg ctactcccaa cccagagcta tcccgcgctt cccgagaatc
     7621 aggtcgtcaa aaatcacctc accggatttt cctgccaagc cggcgcacac cagcagcggc
     7681 atcaaatgct cctcgcgcgg gtggcagtag cgcgcgtaag gggcgctttc ccagttccgc
     7741 agggctgccc ttgtcacggc gtaatccgcc tggcgagagc aggtttcgat cagccattcc
     7801 tggaaggcat cgttggcagg gtcgggcgcg gtccccgggt tccacgagaa agcctgcaaa
     7861 ttatggaaag agaaacccga gccgatgaac agcacttttc cgtcatccag gccgcgcaaa
     7921 gcctctccga gccgcaaatg ctgctccggg tccagcccgc gaactagtga aagctggatg
     7981 gccgggatat ccgcctgcgg gtacatcagg ctgaggggga tgaacaaacc gtggtcaaag
     8041 ccgcgcgtgg ggttgatggc cgccggaata cttgcctctg ccagaacctc acggacgcgg
     8101 gcggcaagct ctgggctgcc cggcgcaggg tagcggatgc ggtaagcctc ttccgggaaa
     8161 ccgtagtaat cgtagaacat cggcggattt tccgcgccga gcaacgtcgg ctgcttttcc
     8221 tcccaatgcg cgctgatgac gagaatcgct tcgggtttga tgagcctggg ggccagcgcg
     8281 gacatgaacc tgaccatcgc gaagtgaccc ggatcgccca aaatgggcag cggaccgccg
     8341 ccgtggctga aataaacggt gtgaaagggc tgctgagata tcatcgaatc ctctttctgg
     8401 tttggttttg cggcgtgtaa ttgcctaaga ttatagtagt taaaacagcc ctctgccgcg
     8461 ctgtaggacc ttcctttcca gtgcccgggt catgaaaaaa ccaagaacag cgggctttgc
     8521 tcatatttga aattgaggct gctggcacag cggaaaatcc atgccgactg ccccgccttc
     8581 ctgtagtaga atccgaccaa cggcgcggag gaaaaatgaa cgggacattc aaggttatct
     8641 ttcatctgga cgattacgac agcgatcgca ttgatatgac atttgccaat attgcgaatc
     8701 tattcgatga catgcaggat ctggacctgg aagtggaact gctggtgaac gggcctgcgg
     8761 tgcaagcctt cacgaatgac aacacgcatg ttctcgagcg cgtgcagcag ttggcggcgc
     8821 tgggtgtgga gactctgctg tgcagaaacg cgctgagggt tcacagcatc ctgcctaaag
     8881 acctgcccgg cgaggtgaag gtggtaccct cgggggtcgg gtggttggtg cgccggcagg
     8941 cggaagactg gagttatatc cggccgtaag cgggattatt ggttctgcca ggtaaactgc
     9001 cggcgacccc tcggatttgt tgcctgagca ataaatattg tcctgcactt tctgctgctg
     9061 gcgcggtaac agccagggct gggcgagcac catttacaaa aataagattt gccactacat
     9121 aaattcttgc gggaaaagtc gttagtttaa aaaccgcgcg cgattgcggg caatatcggc
     9181 agggttcatg gaaatccgct tttcccaagt gaaactagag cgacctcaat tatccgtatt
     9241 acaatttatc cattatgact gcctcagaag ttgaatccac aaacccttct accgggctga
     9301 ctgctgccga agcggcagaa cgattgataa aatttggctt aaacacaatc tctgaagcca
     9361 aacaaaatca actcttgatg tttttaaaga agttttgggc gccggttccc tggatgctcg
     9421 agattaccat tgtgctcgag ctgtaccttg gaaagcatac cgaagcgatc gtgattgggg
     9481 cgttgctcgt tttcaatgcc attctcagct ttgcgcaaga aagccgcgcc cataatgcgc
     9541 tttctcttct gcggcaaaag ttgtcggtac aggttaccac gcgccgcgac ggacaatggc
     9601 gaaggatcca ggctgagaat ctcgtaccgg gagatatcgt ccatctgcgc atgggtgaca
     9661 tcattcccgg cgatgccatt ctcattgatg ggttggtcga gatagaccag tcggccctga
     9721 ccggtgaatc gctgccggtg gagtgcggcg cgggtcaggc ggtgtacgca ggcgcggtta
     9781 tcaagcgcgg cgaaacaact tgcgagatca ccagcacagg agcaagcacc aaattcggga
     9841 agaccgccga attggtgcgc gaggcaaaaa cagtaagtca tcttcaggat attattttta
     9901 ccatcgtgaa atacctggtg attgccgacg tcatactcgt cgcgttggtg atcgcttatg
     9961 ccctgtatgc tcacatcgcc ctgcagatgg tgctgccctt tgcgctcatc attctggttg
    10021 cgtccgtgcc ggtagcgctg cccgccacgt tcacccttgc caccgccctc ggcgcggcaa
    10081 acctggcaaa gcagggcgtg cttgtcgctc gcctttccgc catcgaagaa gccgcagcga
    10141 tgaccatact agccagcgat aagacaggta ccatcaccga gaaccgcctc aaactgagcg
    10201 ctgttcatgc ctacccccct tttcacgtgg aagatgttat tcggtttgga gcccaggctt
    10261 ccagggctgc cacacaagat ccgcttgacc ttgctatcat cgaaggggcg catgaccgtc
    10321 aaattgggct ggtcggagaa cgactcgact acaccccgtt cgatcccgcg acgaaaagat
    10381 ctgaggcgtt gatcgcgaag ccggacggga acacactaag gatcttgaag ggtgctccgc
    10441 tgacgattgc cgccctcgcg ggcggatcgg acattaacgc agacgttgcg aaacttgccc
    10501 tttctgggaa tcgcgtcctg gcagtcgcgg cgggccctga taccggcccc ttccaactgg
    10561 tcggcctgct tggattgcag gatacccctc gcccggattc aaaaagcctg atccatgagc
    10621 ttaataacct gggcgttcga gtggtaatgg tgaccggtga cgatgcgctc accgctaaag
    10681 cggtggccac gcaagtgggg atccccgggg agccgtgccc cgtggaaaag ctgctcggca
    10741 ttcaccagga aggcgctccg gattgcaata tttttgctgg ggtgtttccg gaagacaaat
    10801 tccgcctcgt gcagggattg cagaagtctg gtcaaatcgt gggtatgacc ggcgatggtg
    10861 tgaacgacgc gccagcactg aaacaagcgg aagtgggcat cgcggtcgcg agcgccaccg
    10921 atgtggccaa agccgccgcg agcatagtgc tgaccaaaga tggattaagt aatatcctgg
    10981 acgcaatcga ggccagccgc cggatttacc agcgcatgtt gacctacacc cttaacaaaa
    11041 ttattaagac attccagatt tcactattcc tgagcctcgg atttgtcctg gcccgcgaat
    11101 tcgtagtcac ccccatactg attatcctgc tgctgtttgc gaatgatttt gtcaccatgt
    11161 ctcttgccac tgacaacgtc aattattctc tcaaaccgga tcgctggaaa atcctacccc
    11221 tggttgcagc ttcattcggg cttgcgctgc cggtgttact gttatcattc ggattctttt
    11281 atgtcgccaa ctacgtcctg cgcctcccac tcaatcaact gcaaaccctg atgttcgtga
    11341 tgctggtctt caccgggcag gccaatgtct accttgtgcg ggaacgtcat cacttctgga
    11401 attcccgacc cagccgttgg atgctgcttg gcacgctggc tgacattatt cttgtgctca
    11461 tttttgcctc ccagggtatt ctgatggcgg ctatcccatt atcctatgtc gtcatcagtt
    11521 tgctggtagt ggctgcctat attccatgtg cggattggat taagcttttg gtcttcaagc
    11581 tcaccaaaat tgattaatgc catttggacc tatattcttg cgtagccctt tgatttcaat
    11641 gcgcgtttag gttaaattta tggggtcaat ctccacgcgg cgcattgaga gattcattag
    11701 cttttgcggc taagggtgat agagggattt tattcctttt cggcatgcgt aatcagaccc
    11761 ttggtattgc ccaatgcgag cacaactaat gccgtttacc cgctttcggt atcctaagca
    11821 aaaattccaa caggcccacc ctttttttga gtaaattgtc gggtcagggc cagtcgtttt
    11881 gctttttacg ctgttgaagg atttcgcgat cagttgggct ggcaagctga tgagtttacc
    11941 tccgcaataa agcgtgttat ccaaaaaagg atgctgctcc ggcttttggt atcattcctt
    12001 gccggctctg ctggcactat gctcgatggt ataccatccc agggag
//
LOCUS       DAOQAD010000056        11479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_6419_length_11479_cov_6.899772, whole genome shotgun sequence.
ACCESSION   DAOQAD010000056 DAOQAD010000000
VERSION     DAOQAD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11479
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6419_length_11479_cov_6.899772"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1187)
                     /locus_tag="PKL60_05735"
     CDS             complement(225..1187)
                     /locus_tag="PKL60_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95669.1"
                     /translation="MSLEIILRSGLSTGTILLFAAIGEILTELAGIQNLGVEGMMLLG
                     AMAAFKTSLATGSPWLGLLAGIAAGGLLSLAHAVVTIHFQADQVVSGLSLTFLGTGLA
                     LVLGEGLTGQNAPLVPSLTVPLLSRIPLLGRIFFSDLNLLVYLGYLLIPISWWYINKT
                     RPGMHLRAVGEKPAAADSLGINVYGVRYLYTFVGGCLAGLAGAAISIAISPGWYSNLT
                     TSGQGWIAVALVIFAQWNPLRAALGGYLFGMLRRFMLDLQGPMLLFGLRNPFFYNHNL
                     VFFLQMMPFLLTIIALVWGSASARRQRIGAPGALGLPYIRGERS"
     gene            complement(1184..2293)
                     /locus_tag="PKL60_05740"
     CDS             complement(1184..2293)
                     /locus_tag="PKL60_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95670.1"
                     /translation="MSAPYRLRIQKRTEEVPRWLPLATSVGAIILAFLISGIILLLIG
                     GDPIKTFGYFFNAAFGSWGALSDVVVKATPLLLIGLGCGLAFRMRLWNIGAEGQFYIG
                     AFFASLVPLVPLVNMETTPKFVILLMMALLGMLGGGLYGFIPGWLKAKFNVSEIISTL
                     MLNYVAVFWNNYWIYSVFSDAGFQMTPMFPRSAWLPRLADYAGEVKAFSGITLHLGVV
                     FGLAAAVIIWIIITRSRWGYEINLIGDNPQAARYAGIDITRNVILVFMLSGALAGLAG
                     MSEVSGVVHRLQERISTNYGFSGVIVAWLAKLNPFATILVSFLFGALIVAGREIQPSG
                     LYFFIQGTILFLVISSDVLLRYKIELVRAAPEAIQ"
     gene            complement(2290..3843)
                     /locus_tag="PKL60_05745"
     CDS             complement(2290..3843)
                     /locus_tag="PKL60_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95671.1"
                     /translation="MTNTPELRPQPEEPMNAVEMRDIVLRFPGVLANDHVNFTLRRGE
                     IHALLGENGAGKSTLMNILAGLYKPESGTIKIHGENVVLNSPRDAINRGVGMVHQHFM
                     LVPTQTVTENILLGLDEPGFVMNLPRYDQKVADLAEKYGLKVPPTAKIWQLSVGEQQR
                     VEILKTLYRGAEILILDEPTAVLAPQEIQELMDTLRLMVQQGKSIVFISHKLNEVKQV
                     ADRLTVLRKGKVTADGINLEDVDKETLAELMVGRKVIFQLNKKPHTPGELVLDVQNVS
                     ALNNRGLPALKNISFQLRQGEILGIAGIAGNGQSELAEVITGLRPCQGKILVNCTDVA
                     NQSPKRAIDQGTAHIPEDRTGVGTAPGMALTGNMIMKSYDRAPISQKWRISYTAAREF
                     ASTLKEKYDIQAPSLTTQVNKLSGGNLQKVILAREISCGPKLIVAVQPTRGLDVGAIE
                     GIQNLLLEQRENDSAILLISEELDELLELSDRIAVIFEGELAGEFPTEEADLKTIGLM
                     MTGEYRRQE"
     gene            complement(3964..5202)
                     /locus_tag="PKL60_05750"
     CDS             complement(3964..5202)
                     /locus_tag="PKL60_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNW95672.1"
                     /translation="MKKGIWLFNLFLVLALVLTACAKTPAETEAPAVDCRAQTTSEAV
                     GSYQVPAPIEGCYNVAFVYVGPHDDGGWSQSHDVGRLYVEQNLEGVHTAYVENVAEGA
                     DAEQVTRSLARKGFDVIFTTSFGFMDSSETVAGEFPDVDIIHISGYKANGANFGNLMG
                     AMEDIKYLAGMLAGSRATLDGNPKLGYMATFPIPEELRLGNAFALGVQKTCPECTIDV
                     RFINTWHDPILEQEGAKSLFDAGAQVVMTGADTPAPALAAPAGKWGITYDYKGNCTLD
                     TCLTSMYWNWGVIYADIVEKSRAGTWKGGWEYFDGDSGGLGLYGFMEGETMMPGVAEL
                     PEADLQLVRETLDKMLKGEFTRFDVFKGPITDNQGNLILPDGVSMEQLDLDGFKQFGS
                     ECKTCMYWWNENISAELPDL"
     gene            complement(5513..5845)
                     /locus_tag="PKL60_05755"
     CDS             complement(5513..5845)
                     /locus_tag="PKL60_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95673.1"
                     /translation="MKKRVLLVISIVVILMGVMALIPGLKLGTEPAWHAAIKIVVGLV
                     GLWVSLKKTGKTKTALLVIGILLAVMGLVDVVGLLNLGTEPVWHSIVKIVIGAVAIYL
                     GATNLKKE"
     gene            complement(5971..6639)
                     /locus_tag="PKL60_05760"
     CDS             complement(5971..6639)
                     /locus_tag="PKL60_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95674.1"
                     /translation="MPNAWPGQFVMAWLPGIGEKPFSISGNSPLSLTVCDVGPVSHAL
                     CQIKAGDQLWVRGPLGQGFTLSRQAHILVGGGYGAAPLHFLAKQALALGHEVTACLGA
                     KTRADLIMVEQFEELGCPVLIATEDGSAGVQGLVSLPLLHALGQNPRAGVYACGPNGM
                     LLAVHRLCQSVQVAVQLSFEALIRCGIGLCGSCELSEETCQLLGIPSGFLVCHDGPVV
                     KTGS"
     gene            complement(6726..7655)
                     /locus_tag="PKL60_05765"
     CDS             complement(6726..7655)
                     /locus_tag="PKL60_05765"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNW95675.1"
                     /translation="MTQARLSCGFLGLTLRNPLVLASGILGTSPSLLERAAREGAGAV
                     TTKSCGPEPRAGHPNPVALAWDGGVINAVGLTNPGAQEEVPLLRAAKQRLAALGAPLF
                     ASIFAPTTELFGEVARVIAAAEPDLIEVNISCPNVASEFGTPFSAAAGSAAAVTRAVR
                     KAVSLPISIKLAPNVANIGQIARAVADEGADAVTAINTMPAMLIDAAAGKPVLYNRSG
                     GLSGPALKPIALRCVYEIARTVSLPIIGTGGVSTGTDAAEMLLAGASLVGIGSAVWQD
                     GPAAFARIGAELRTFMQAAGYETVEALRGRAAA"
     gene            complement(7652..9046)
                     /gene="pyrF"
                     /locus_tag="PKL60_05770"
     CDS             complement(7652..9046)
                     /gene="pyrF"
                     /locus_tag="PKL60_05770"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNW95676.1"
                     /translation="MNFIEKLEAAVEKNGSLLMVGLDPQEEFLPGSGDVCTRLTDWGK
                     GIIEQTSDLVCGYKPNMAFYEQYGPAGLRALQETIQAVPTDLPIVLDAKRGDIGSTAQ
                     AYARAAYEQFHADAVTLSPYLGQDSIKPFLADPEKFAFILCQTSNPSANEIQGFGTPA
                     LYEYIARVSRNWGTAGQVGLVIGATQPEALQRVRAICPEAWFLAPGVGAQGADLKTAL
                     TAGLRADKKGILVPVSRAVIAAAAPRKAAEALREQINAAIVEITPTPLDPFRALVNGL
                     FSSGCVKFGSFTLASGKQSPVYLDLRRLVSFPDVLDMAVEAYIDRLISLEYDCLAGVP
                     YAALPIAAIAASRLKRPMVYPRKEAKTHGTSQMVEGVFQPGQRTVLLEDVITSGGSIL
                     TAAETLSAAGLSVRDAVVLVDREQGGTDALARQGIQAHAVLSMRQILTRLQAQDLIDQ
                     STYQEVSRYLDGEA"
     gene            complement(9112..10107)
                     /gene="pyrB"
                     /locus_tag="PKL60_05775"
     CDS             complement(9112..10107)
                     /gene="pyrB"
                     /locus_tag="PKL60_05775"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNW95677.1"
                     /translation="MMTENFEPLFKLDEYQREAKHKSVFYQQDILSVNQFTKEDMDYL
                     FQRADEMLAMCRRWGTCDLLKDYTLACVFYEPSTRTSSSFISAMVRLGGRVIPITEGI
                     QYSSVSKGESLIDSMLTLEQYCDLIVLRHPEIGSSATAAKYVKVPIINAGDGAGEHPT
                     QALLDLYTIKSELGTLDGLKVAMVGDLRYGRTVHSLTRLLSQYDVSLRFVSPDTLRLP
                     LELMNDLIDKGVKVRETNKVADVIENADVLYVTRIQKERFSDLAQYEEVKNYFEITPE
                     LMEQAKQKMIVMHPLPRVGEIHYKVDRDPRAAYFRQVQNGLYIRMALLAAVLGQA"
     gene            complement(10131..11168)
                     /locus_tag="PKL60_05780"
     CDS             complement(10131..11168)
                     /locus_tag="PKL60_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNW95678.1"
                     /translation="MILPGLVDVHVHLRSPGGEHKEDFRTGSAAALAGGFTTLLAMPN
                     TQPPLVSYDIWRVAQTQANSQSLCDVFLFAGASAEHLDQLPLLSGHAPALKIYLDQTY
                     GPLRVRGLDALAGVMAGWQADKPICLHAEAESVAVGIALAALYGKHIHFCHVSRQAEI
                     ELIARAKERGLPVTCEVTPHHLFLTEKDAVRLGPYGDMRPTLAAPSDQAALWEHINST
                     IDCIASDHAPHTRAEKERPEGAPPGVPGLESTLPLMLTAVSQGRLSAARLEELLARNP
                     RRIFKLPPQPDTWVEVDPGIESVFPERPLYTKCGWSPFAGMRLRGRITSVVLRGQEVV
                     RDGIVLNYQKP"
BASE COUNT         2449 a         3331 c         3284 g         2415 t
ORIGIN      
        1 aaaacggttc gcaatgacga atcttccccg agcgccagcg cggcgatctc cctgccagca
       61 ataaggccgc cgcacccagc ctaccaactg cgcccagcct gacattccaa atttgtcacc
      121 ctgagcgcag tgaaggatcc tggtttgtcc ccgggaaaaa aagaatcggt ctgtggaacc
      181 aaaacgagga ggcagcacgc ctcctcgttc cctaaaccgt aatttcagct ccgctccccg
      241 cgaatatacg ggaggccgag cgcgcccggc gcaccgatgc gctgcctgcg cgcggacgcg
      301 gaaccccaca ccagcgcgat aatcgtcagc aggaacggca tcatctgcag gaagaaaacc
      361 aggttatggt tgtagaagaa aggattgcgc agcccgaata agagcatcgg accctgcaag
      421 tcgagcataa agcggcgcag cataccaaac aagtagccgc ccaaggccgc ccgcagcggg
      481 ttccactgcg cgaaaatgac cagcgccaca gcaatccagc cttgtcctga agtggtcaga
      541 ttgctgtacc agccagggga gatggcgatg ctgattgctg cgccagccaa accggccagg
      601 cagccgccca caaaggtata gaggtagcgc acgccgtaga cgttgatgcc caggctgtct
      661 gccgcggcag gcttttcccc caccgcgcgc aagtgcatcc ccgggcgggt cttattgata
      721 taccaccacg atatgggtat cagcaaataa ccgagatata ccaatagatt caaatctgaa
      781 aagaaaattc gtcccaagag cggaatcctg gaaagcaggg gaaccgtcag gctggggacg
      841 agcggcgcgt tctgcccggt taggccctcg cccaatacca gcgccaaacc ggtgcccaga
      901 aaggtgagcg atagaccgct gaccacctgg tcggcctgga agtgaatggt gacgacggca
      961 tgcgccaggc tcagaagccc gcccgcggcg atgcccgcca gcaagcccaa ccagggactg
     1021 cccgtcgcca gggaggtctt aaaggctgcc attgccccga gcagcatcat gccttccacg
     1081 cccaggttct gtatcccggc aagttcggtc agaatttccc cgattgccgc gaacagcaga
     1141 atagttcctg tggagaggcc ggatctgagg atgatttcca ggctcactgt attgcctccg
     1201 gagccgcacg taccagctca attttataac gcaggagaac atcgctgctg attaccagaa
     1261 agagaatagt tccctggatg aagaagtaca gaccggatgg ttggatttcg cgtccggcga
     1321 caatcagcgc cccaaacagg aaggaaacca ggatggtggc gaatggattg agcttggcca
     1381 gccaggccac aatcacgccg gagaagccgt agttggtcga gatgcgttcc tgcaggcggt
     1441 ggaccacgcc gctgacctcg gacatgccgg ccaacccggc cagcgcgccg gagagcatga
     1501 agaccaggat gacgttgcgc gtaatgtcga tgccggcata gcgggcagcc tgcggattgt
     1561 caccaatcag gttgatttcg taaccccagc ggctgcgcgt gataataatc cagatgatga
     1621 ccgcggctgc caacccaaag actaccccaa ggtgcagcgt gattccggag aatgctttca
     1681 cttcgccggc gtagtccgca aggcgcggca gccaggcaga tctcgggaac attggcgtca
     1741 tctgaaagcc ggcgtcgctg aagacgctgt aaatccaata gttgttccag aatacggcca
     1801 cgtagttcaa catcagtgtg gaaataattt cgcttacgtt gaattttgct ttcaaccaac
     1861 ccgggatgaa gccgtacaag ccgccgccga gcatcccaag cagcgccatc atcagcagaa
     1921 tcacaaactt gggcgtcgtt tccatattca ccagcggcac cagggggacc aggctggcaa
     1981 aaaacgcgcc tatatagaat tgaccttctg cgccgatatt ccacaggcgc atgcggaatg
     2041 ccaatccgca gcccagacca atcagcagca gcggcgtggc tttgaccacc acatccgaga
     2101 gagcacccca actgccgaaa gccgcgttga aaaagtaccc gaaggtcttg attgggtccc
     2161 cgccaatcaa cagaagaata attccgctga tgagaaaagc caggattatc gcgccgaccg
     2221 aggtcgccag aggcagccag cgcgggactt cctctgtgcg tttttggatg cgcaggcggt
     2281 atggcgcgct cattcctgcc tcctgtattc acctgtcatc atcagaccaa tggtctttag
     2341 gtctgcttcc tccgtaggga attcccctgc caattcgcct tcaaagatga ccgcaatgcg
     2401 gtcagaaagc tccagcagtt catccagttc ctccgagatc agcaggatgg ccgaatcatt
     2461 ttcgcgctgc tccaggagaa ggttttgaat gccttcaatc gctcccacgt ccagcccgcg
     2521 cgtcggctgc accgccacaa tcagtttggg tccgcaggaa atttcgcggg caaggatcac
     2581 tttttgcaag ttccctcccg aaagtttgtt gacttgcgtg gtgagactgg gcgcctgaat
     2641 atcgtatttt tctttgagag tactggcaaa ttcccgggcg gcggtatagc tgatccgcca
     2701 tttctgactg attggggcgc ggtcgtagga tttcataatc atgttgccgg taagtgccat
     2761 cccaggcgcg gttcccacgc cggtgcggtc ttccgggata tgcgccgtgc cctggtcaat
     2821 cgcccgcttg ggggattgat tggctacgtc ggtgcagttc accagaattt ttccctgaca
     2881 gggacgcagc ccggtgatta cttccgccag ttcactttgt ccgttgccgg caattccggc
     2941 aattccaaga atttccccct gccgaagctg gaaggagatg ttctttagcg ctggcagacc
     3001 gcggttattg agagcggaaa cgttctgtac gtcgagaacg agctcccccg gagtgtgggg
     3061 ctttttgttt aactggaaaa tgactttccg gccgaccatc agttcagcca gggtttcttt
     3121 atccacgtcc tcaaggttta tcccgtctgc ggttaccttg cctttgcgca gcacggtcag
     3181 ccggtctgcc acctgtttta cttcgttcaa cttatggctg ataaacacga tggatttccc
     3241 ttgctggacc atcaaacgca gcgtgtccat cagttcctga atttcttgcg gggcaagcac
     3301 ggccgtcggt tcgtccagaa tcaggatttc ggctccgcgg tacagggtct taaggatctc
     3361 cacgcgctgc tgctcaccta ccgaaagctg ccagatcttg gctgtcggcg gcactttgag
     3421 cccatatttt tcagccaaat ccgccacttt ttggtcataa cgcggcagat tcatgacgaa
     3481 gccgggttca tccaggccaa gcagaatatt ctcggtcacg gtttgcgtgg ggacgagcat
     3541 gaaatgttgg tgaaccatgc caacgccgcg gttgatagcg tcccgagggg aattcagcac
     3601 cacgttctcc ccgtgaattt tgatcgtgcc ggattcgggt ttgtaaagcc cggcaaggat
     3661 gttcatcagc gtgcttttcc cagcgccgtt ctcccccagg agggcgtgaa tttctccgcg
     3721 cctgagggtg aaattgacgt ggtcgttggc aagcacgcct ggaaagcgca gtacaatatc
     3781 gcgcatttcc accgcattca tgggttcctc aggttgtggg cgtagctcag gtgtgtttgt
     3841 catgcgttct ttcagggtaa aatgctctgc aagcctcttg ccagttcaag gctcatactg
     3901 attcacaatt caagttccag gggatgaccg atgggtcatc ccccgggatt atctcgtcag
     3961 attctacagg tcaggcagtt ccgcggagat attctcgttc caccagtaca tgcaggtctt
     4021 gcattcgctg ccaaactgtt tgaacccgtc caggtccagt tgttccatac tgacgccatc
     4081 ggggaggatc aggttgccct ggttatccgt gattgggcct ttgaagacgt caaagcgagt
     4141 gaattcaccc ttgagcatct tatccagggt ttcgcgcacc agctgcaggt cagcttccgg
     4201 aagctccgcc acaccgggca tcatcgtttc gccttccata aagccgtaca gaccaagccc
     4261 gccggagtcg ccgtcgaagt attcccagcc gcccttccag gtgccggcgc gggatttctc
     4321 tacgatatcg gcataaatca caccccagtt ccagtacata gaggtcaggc aggtatccag
     4381 tgtgcagttg cccttgtagt cgtacgtgat gccccatttt cctgcggggg ctgccagggc
     4441 aggggcgggg gtgtcggcac ccgtcatgac aacctgagcg ccagcatcaa agagcgattt
     4501 cgcgccttcc tgctcgagga tcgggtcgtg ccaggtgttg atgaagcgca cgtcaatggt
     4561 gcattccgga caggtcttct ggacccccag cgcgaaggcg ttgcccaggc ggagttcctc
     4621 ggggatgggg aaggttgcca tataacccag cttggggttt ccgtccagtg tggcgcggga
     4681 gccagccagc atgccagcca ggtacttgat atcttccatg gcgcccatca aattgccaaa
     4741 attcgcgcca ttggctttgt aaccgctgat gtggatgata tccacatccg ggaactcgcc
     4801 ggcaaccgtt tcagaggagt ccatgaaacc aaacgaggtg gtgaaaatca catcgaagcc
     4861 tttgcgtgcc agggagcggg taacctgctc ggcgtcggcg ccttccgcca cgttttcaac
     4921 gtacgcggtg tgcacgcctt ccagattctg ctccacgtaa aggcggccaa catcatgcga
     4981 ctgcgaccac ccaccatcgt cgtgcggtcc aacatacacg aacgcgacgt tgtagcagcc
     5041 ttcaatcggc gcagggacct ggtaggaacc tacggcttcg gaggtggtct gggcgcggca
     5101 gtccacagcg ggagcttccg tttcagccgg ggtcttggcg caggcggtca gcacaagcgc
     5161 gaggaccaag aataagttga acagccaaat accttttttc atcttttctc ctgtttagat
     5221 tgtttgaacg gaatggaaaa atgcaattgc gaaagacttg ctcctttcgg ctaacaaaac
     5281 gacggctgct taattgtact gcaatcaaga caaaaactca gaaactgcgg gggaatttcc
     5341 tggaattatt tcctggtata tccgtcttat cgggaataag aaaaggtatt tggagagagt
     5401 ctgatgatgt cgcatactga acagcgcaac gaaaaacagc aggtgtaaac ctgctgtttt
     5461 ctgaattctg acaaccccgc cccagccatg gaacggggtt gccgttcagg tcctattctt
     5521 ttttcaggtt ggtcgccccg aggtagattg cgactgcgcc gatcacgatc ttgacgatgg
     5581 agtgccagac cggttccgta cccaggttta gcaggccaac gacatccacg agacccatga
     5641 ctgccaacag aataccgatg accagcaggg cggtcttggt cttaccggtt ttctttaggg
     5701 agacccatag accgaccaga ccgacgacga ttttgatggc agcatgccag gcgggttcgg
     5761 tgcccagctt aaggccgggg atgagagcca tgacgcccat caagattaca acaatactga
     5821 ttacaagcaa tactcttttt ttcataatag ttctcctgta tgaatggtgg tttgagcttt
     5881 ttaggctccc aaggatagaa aatattgttt catgggtatg atgaatacga ttattttcgg
     5941 caactattct tgagtagaac tttcaagaat tcaacttccc gtttttacca ccggtccgtc
     6001 gtggcatacc agaaaaccgg atggaattcc caggagctgg caggtctctt cgcttagctc
     6061 gcacgagccg cataagccaa tcccacagcg gatgagcgct tcaaaagata gctgcaccgc
     6121 aacctgaacg ctttgacaaa gccggtgcac agccagcagc ataccattcg gaccgcaggc
     6181 gtacacgcca gcccggggat tctgccctaa agcatgcaac agcggcagac tgaccaagcc
     6241 ctgtacgccc gcgctgccgt cttcagtggc aatgagaacc ggacagccca attcttcaaa
     6301 ctgttcgacc atgattaagt cagcccgggt cttcgcgccc aggcaggcgg tcacttcatg
     6361 ccccagcgcg agggcttgct ttgccaggaa gtgcagcggc gcggcgccgt agcccccgcc
     6421 aaccagaatg tgcgcttgcc tggataaggt aaagccctgc cccaaaggac cgcgcaccca
     6481 cagctggtct ccagccttta tctggcagag ggcatgactt accggaccga cgtcgcacac
     6541 ggtcagggac agcggggaat ttccgctgat gctgaagggc ttttcgccga tacccggcag
     6601 ccaggccatc acaaattgcc cgggccaggc gtttggcaag ccggcatcta atattaaagt
     6661 ctgggtacgc gcattttcct ggcgaaccga ttgcacccga aaaactgaaa agagcccttt
     6721 ctggctcatg ctgccgctct cccacgcagc gcctcgaccg tttcgtatcc ggctgcctgc
     6781 ataaaggtcc gcagttcggc gccaatccgg gcaaacgctg ccggcccgtc ctgccagaca
     6841 gccgaaccaa tcccaaccag gctggccccc gccaaaagca tctcggcggc atcggtccct
     6901 gtggaaactc cgcctgtgcc gatgatcggc aagctgaccg tccgggcgat ttcatacacg
     6961 cagcgcagcg cgatgggttt cagcgccggc ccggagagcc cgccgctgcg gttgtacagg
     7021 acgggtttgc ccgctgctgc gtcaatcagc atcgcaggca tggtgttaat cgcggtgacg
     7081 gcgtccgcgc cttcatccgc aactgctctg gcgatttgac cgatgttggc cacgttaggc
     7141 gccagtttga tgctgatggg caggctgacc gctttcctca ccgcgcgggt gacggcggcc
     7201 gcgctcccgg ctgcggctga gaagggagtg ccaaactcac tggcgacgtt tgggcaggag
     7261 atgttaacct caatcaggtc tggttccgcc gcggcgataa cccgcgcgac ctcgccaaac
     7321 agctccgtgg tgggcgcaaa gatgctggca aaaagcggcg cgcccagggc agccaggcgc
     7381 tgtttggctg cccgcagcag aggaacttcc tcttgcgcgc ctgggttggt cagtccgacc
     7441 gcgttgataa ccccgccgtc ccatgccagg gcgaccgggt tgggatggcc cgcccggggt
     7501 tctggtccgc agcttttggt cgtgactgcc cccgcgcctt cccgggccgc cctttccagc
     7561 aggctggggc tggtgcccag gataccggat gccaacacca gcgggttgcg caaggtcagc
     7621 ccgaggaatc cgcaggagag ccgggcttgg gtcatgcttc cccgtccagg taacggctca
     7681 cttcctgata ggtactttgg tcaatcaagt cttgtgcctg caggcgtgtg aggatctggc
     7741 gcatactaag cactgcgtgc gcctgtattc cctgccgggc aagggcgtcc gtgccgcctt
     7801 gctcgcggtc caccagcacc accgcgtccc gcacgctcaa acccgcggcg ctcagggtct
     7861 ccgcggctgt caggatgctg ccgccgctgg taatgacatc ttccaacagc accgtgcgct
     7921 gccccggttg aaacacgcct tccaccattt gcgaggtgcc gtgggttttg gcttccttcc
     7981 gtggataaac catcgggcgt ttcaagcggg aggcggcaat cgcggcgatg ggcagcgcag
     8041 cgtagggcac gccagccagg cagtcgtact caagggatat cagccggtct atgtaggctt
     8101 ccaccgccat atccagcacg tccgggaaag aaaccaggcg gcgcaaatcc aggtagaccg
     8161 gcgattgttt gccggatgcc agggtgaaac tgccaaattt tacgcagccg ctggaaaata
     8221 atccgttcac gagcgcgcgg aatgggtcaa gcggggtggg ggtaatttct acgatggccg
     8281 cgttgatttg ctcgcgcagc gcttccgcgg ctttgcgcgg cgcagcggct gcaattacgg
     8341 cgcgggagac aggcacgaga atgcctttct tatccgcgcg cagtccggca gtcagggcgg
     8401 tcttaagatc cgcgccttgt gccccaaccc ccggcgccag gaaccacgcc tccgggcaaa
     8461 tcgcgcgcac gcgctgcaga gcttcgggct gggtggctcc gatgaccaga ccgacctgcc
     8521 cggccgtgcc ccagttccgg ctgacacgtg cgatgtattc atacaaagcc ggcgtgccga
     8581 agccttgaat ctcgttggcg gaggggttgg aggtttggca gaggataaag gcgaactttt
     8641 cggggtccgc aaggaagggc ttgatgctgt cctgaccgag ataaggactg agcgtgaccg
     8701 cgtcggcgtg gaattgttcg taagcggcgc gggcgtaggc ttgtgcggtg gagccgatat
     8761 ctccgcgctt ggcgtccagc acgattggaa ggtcggtcgg tacggcttgg atggtttcct
     8821 gcagcgcgcg cagcccggcg gggccgtatt gttcgtagaa ggccatgttg ggcttatatc
     8881 cgcacaccag gtcgctggtc tgctcaatta tccccttgcc ccaatcggtc agacgggtac
     8941 aaacgtcccc gcttccgggc aggaattcct cctgcgggtc caacccaacc atgagcaagc
     9001 tgccgttctt ttcgaccgct gcctctaatt tctcaataaa gttcatcgcg tctcctgtag
     9061 tgctgttcac cgactctgcg ccgtctgcgg ggctttgcct gtcttttcgg gctatgcctg
     9121 gccaagcacg gcagccagca gcgccatgcg gatgtagagg ccgttttgaa cctggcggaa
     9181 gtacgccgcc cgcgggtcgc ggtccacttt gtagtgaatc tcgccgacgc gcggcagagg
     9241 gtgcatcacg atcatctttt gcttggcctg ttccatcagc tcgggtgtaa tttcaaagta
     9301 gtttttgacc tcctcgtact gcgccaggtc gctgaagcgt tccttctgga tgcgggtaac
     9361 gtacagtaca tccgcgtttt cgatgacatc cgcgactttg ttggtctcgc gaactttcac
     9421 gcccttatca atcaggtcgt tcatcaattc cagaggcagg cgcaaggtgt cgggggaaac
     9481 aaagcgcagc gagacgtcgt attgggaaag caggcgcgtc aggctgtgga cggtgcgccc
     9541 gtagcgcaaa tcaccaacca tagccacttt caacccatcc aaggtgccga gttcgctctt
     9601 gatagtgtac aggtccagca gcgcctgggt ggggtgctcg ccggcgccgt cacccgcatt
     9661 gatgattgga accttcacat acttcgccgc ggtggcggaa gagccgattt cgggatggcg
     9721 caggacgatg aggtcgcagt actgctccaa cgtgagcatg ctgtcgatca gcgattcgcc
     9781 tttggagacg gaggagtact gaatcccttc cgtaatcggg attacccggc cgcccaaccg
     9841 caccatcgcg gagatgaagg aggaactggt gcgcgtgctg ggttcgtaga acacacacgc
     9901 cagagtgtaa tccttgagca ggtcgcaggt gccccagcgg cggcacatgg ccagcatttc
     9961 gtccgcccgc tggaacaaat aatccatatc ctctttggtg aattgattca cggagaggat
    10021 gtcttgctgg taaaacacgc ttttgtgttt ggcttcgcgt tggtattcat ccagcttgaa
    10081 aagaggttcg aagttttcag tcatcatgtt gtcagctcca aaagtgaaaa ttaaggtttt
    10141 tgataattta aaacgatgcc atcgcggacg acttcctgtc cgcgcagtac cacactcgta
    10201 atcctaccgc gcaggcgcat gccggcaaaa ggggaccaac cgcatttggt gtaaagcggg
    10261 cgctccggga aaacgctctc aatccccggg tccacctcca cccaggtgtc gggctgcggc
    10321 ggaagcttaa aaatacggcg cgggttgcgc gccagcagct cttccaggcg ggcggcgctc
    10381 aggcggcctt gcgataccgc cgtcagcatc aggggcagcg tggattccaa gcccgggacg
    10441 cccggcggag cgccttccgg gcgctctttt tccgcgcggg tgtgcggggc gtgatcgctg
    10501 gcgatacagt caatcgtgct gttaatatgc tcccagagcg cagcctggtc gctgggggca
    10561 gccagggtag ggcgcatatc cccgtacggc ccaagccgga cggcgtcttt ctcggtcagg
    10621 aacaaatggt gcggggtgac ctcgcaagtg actggcaggc cgcgttcttt cgcgcgggca
    10681 atcagttcaa tttccgcctg gcgcgatacg tgacaaaaat ggatgtgttt cccatacagc
    10741 gctgccagcg cgatgccgac cgcgacggat tctgcttctg cgtgcagaca gatgggcttg
    10801 tcggcctgcc agccggccat cacgcctgcc agtgcgtcca agccgcgcac ccgcagcggt
    10861 ccgtaggtct ggtccaggta gatttttaga gctggagcat gcccggaaag cagggggagc
    10921 tggtccagat gttccgctga agcgcccgcg aataaaaaaa catcgcacag actttgcgaa
    10981 tttgcttggg tctgtgcgac gcgccaaata tcataggaga ctaaaggcgg ctgcgtgttg
    11041 ggcattgcca acagggtggt gaaaccgccg gccagcgcgg cggcgctgcc ggtgcggaaa
    11101 tcctctttgt gctcaccgcc cggactgcgc aggtgaacat gcacgtcaac caaacccggt
    11161 aagatcatca gaccacctgt aagtaaacta aactattata cggaaacagg cgcatttccg
    11221 tcaagttttt ttatgtgtaa gcgcaaagga attgtaagcg caacatgata atgtcctaaa
    11281 gtagcaaaat agaaatgtcc tcattaagat taagagctat gcaaacctca tttgattccc
    11341 ccaggttttt ttacaaagga ataaacctac cctgatacac aaaggaattg cagcctttct
    11401 actcttactc ccctcccagc gaatgaaaat taactgatga tcctactgaa cgatcgctgg
    11461 gacggtgagt tgaaacgaa
//
LOCUS       DAOQAD010000057        11444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_6465_length_11444_cov_5.631574, whole genome shotgun sequence.
ACCESSION   DAOQAD010000057 DAOQAD010000000
VERSION     DAOQAD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6465_length_11444_cov_5.631574"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKL60_05785"
     CDS             complement(<1..230)
                     /locus_tag="PKL60_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNW95679.1"
                     /translation="MPEFPEVETIRRGLLTGDASTPPLLGQRAASVSILWERSIASPS
                     PRELAERISGKTVRGIERRGKFLLIHLEEEFL"
     gene            complement(255..1244)
                     /locus_tag="PKL60_05790"
     CDS             complement(255..1244)
                     /locus_tag="PKL60_05790"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNW95680.1"
                     /translation="MTSPNMRHDLLYGNIRLFAGTSTPELAQEISDYLGTPLCGRDVI
                     EFTNENLFVKLHASVRGQDCYIIQTTSIPVHRNLMELLIMTQTLRLDSAGRITVVVPY
                     MCYGRSDRKDQPRVPITARLVADMIQVAGADRYITMDLHAGQIQGFFSIPGDVLSGYH
                     IIKPYLMDLLPNMNRPVMLTADLGFAKRGRRYAQALNIPLAFIEKRRVDNEDHTEALS
                     IIGQVSGRDVILIDDEIDTGGSICEAVNLAKQYGAGEIYVVFVHPVLSANAVERLSNL
                     PVRQFITTNTIPISAEAKALFGDRLTILSIGSLLGEVIKRANEGSSVGAMFNE"
     gene            complement(1245..1826)
                     /locus_tag="PKL60_05795"
     CDS             complement(1245..1826)
                     /locus_tag="PKL60_05795"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNW95681.1"
                     /translation="MPSITSERLRLRAAEKADIPMFIRWISDPEVTEHLLLRLPMSLA
                     EEEIWFENMIARPAAEHVYVIEARIPPSEAGAEAQWLPIGNTSFISILEIDRCAEIGI
                     MLGEKSWWNKGYGTETMRTMLRHGFETLNLHRIWLQVYANNQRGMRAYEKAGFRREGI
                     YREGHYQAGKYHDIILMSILRQEWDSQYLVKGE"
     gene            complement(1836..3155)
                     /gene="aspS"
                     /locus_tag="PKL60_05800"
     CDS             complement(1836..3155)
                     /gene="aspS"
                     /locus_tag="PKL60_05800"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HNW95682.1"
                     /translation="MEKTPIRDLKAHIGEVVKLQGWLQTLRDQKSMQFLILRDRTGLV
                     QITNWKKGSPELAALVSTLSTEAALSVTGKVVENEVVKLGGIEVQLESLTVENNAENP
                     LPFDPFAETLPDQDYRLDWRFLDLRRERNRLIFEVETTLEAAMREYWLENGFIEMHSP
                     KLMGAPSESGAELFSLKYFETTAYLAQSPQFYKQMAMAAGYEKVFEIGPVFRADPSFT
                     SRHMTEFTGVDFEISWIESHEDVMSFSERWLAHTYKRVQEKHGEAIKNVFGVDLQVPE
                     VPFPRLTMAEAYQILKDQGYKLPPERKGDLDPGAERALGAYIKEKTGSDFVFVKDWPF
                     SVRPFYHMVYEDNPALTKSFDLLGRGLEITTGAQREHRYEVLKNQALDKGLTLPPIQN
                     YLNYFRYGCPPHGGLGLGLSRLLMVMLDLPNIREAVYLFRGPNRLEP"
     gene            complement(3188..4198)
                     /gene="meaB"
                     /locus_tag="PKL60_05805"
     CDS             complement(3188..4198)
                     /gene="meaB"
                     /locus_tag="PKL60_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNW95683.1"
                     /translation="MTDFSVEAVRKGDKLALSRVLSRVEADDEDGRAALDALFAFTGH
                     AHKIGVTGAPGSGKSSLVNALAHYLRRTFPDSRVAIIAVDPTSPFTGGAILGDRVRMP
                     DLQNDTGVFIRSMATRGALGGLAEATLALSQVFDAAGYDTVLIETVGAGQAEVDIVNL
                     AHTTIVVDTPGMGDDIQTIKAGILEIADILVVNKAELPGADQTYRNLVSMIEMGYRGS
                     GGINGGHHRLSEAPEAQTPDHAGWLPPVLRTTAITAQGIPELEQAIQRHRAYSKASGS
                     WHTKESSAIQQTIDKLITDELIGNWKKKLPSGLYETTLQAVSARKMSPRAAARALLAY
                     NS"
     gene            complement(4202..4606)
                     /locus_tag="PKL60_05810"
     CDS             complement(4202..4606)
                     /locus_tag="PKL60_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNW95684.1"
                     /translation="MPEKKIRVLIAKPGLDGHDRGAKVIARALRDDGMEVIYTGLRQT
                     PQMIAEAALQEDVDVVGLSILSGAHNALVPQVLEKLKENGQDDVFVFLGGIIPERDIP
                     GLLAEGVTKVYGPGTDTRVVCQDIRAALAERA"
     gene            complement(4694..5224)
                     /locus_tag="PKL60_05815"
     CDS             complement(4694..5224)
                     /locus_tag="PKL60_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HNW95685.1"
                     /translation="MTLQTISPKELLVKPYDLIERQTLVLTSGSFAQRNFNAMVIGWG
                     FLGCMWNRPCVIVPVRPTRYTYNFIENAPDFTLCAFPEAYKEDVLFLGTHSGRNEDKL
                     SRTRLTPQASQAVSAPSFAEAELVIECKKIYRADLDPSCFLDPSIGRHYPLKDFHRMY
                     YGEMVAVRAEPGFLAA"
     gene            5325..6281
                     /locus_tag="PKL60_05820"
     CDS             5325..6281
                     /locus_tag="PKL60_05820"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNW95686.1"
                     /translation="MKKDTKINWNLIIRWAGTLLSIGILIYLFRQIGAEETWAAVQKI
                     SGRRIAEVLVLVFVSRLATWGRWHTLLQVQALRVRWQDSLRLTFAGLFASNVLPTTIG
                     GDVVRLAGAVRLGIDAALAAASLVADRLVGMTGMALALPFAIPALSSYLKAQASSGAA
                     QTTGAAALFPAFFRKIQQAIKKVFSNFRFWLKHPLILAQALGFTFIHMLATFLIVQIL
                     VQGMGESISFTKTAGLWSLTYFISLIPVSINGLGLQELTITNLFSALGGLQPSTSISL
                     AVILRVVWMIGSLPGAFFIGGILGGKRPSEAGGEKQNGNTQD"
     gene            6389..8203
                     /locus_tag="PKL60_05825"
     CDS             6389..8203
                     /locus_tag="PKL60_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95687.1"
                     /translation="MPVLALGVFLMQRLIQTPEKLKHKVPRWLAYGISALLQIGLLAV
                     LLTQDFQLIHSTIGRLFLSVLAAGGAALLFLTTEKPGSPQRSFITALLVFGALYRAAA
                     FLPELQPGPFALGWSEGSRFYNASLFLSGRIYGQKLPLPVLHPSRYLLQAFPFLLGIK
                     SILAHRVWQVLLWLGMSAWGAALFARRLGKDQGISFGLLTIWAALFFFQGAVYYHLMG
                     CVILVLLGYRKDKIWRTLAFVILASLWAGISRVNWMPLPGLLAGALYLLDEPLGQKKW
                     LKYLAPVLLWAVLGFASAWLAQQGYIRISGEDPALFNSAFSSALIWNRLLPNPTFGLG
                     ILPAILLVCLPGMGLLAQRVFARQAPALHWLRWLGLAGILGAFFLGGLLVSLKIGGGG
                     DLHNLDGFLVFWVLIAGSLLAGAYGAETPQNAPAPGIRPAFSWLILAALIPLYFALSH
                     SAVWGFTPAADQRVEVARMQAALDLLKDEPGEVLFISERQLLSFNEIQGVRVVPEYEK
                     VFLMEMAMGNNQPYLQAFYADLTGRRFKAIITDTISTRIQGDGKSFGVENDAWVQKVL
                     EPMLQTYEPVLSWNNASAHLLIPQGQDALKQALLELQP"
     gene            complement(8210..9172)
                     /locus_tag="PKL60_05830"
     CDS             complement(8210..9172)
                     /locus_tag="PKL60_05830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW95688.1"
                     /translation="MKLSLVVPVYNEEENLPELLPRIYEAVAPLEMDWDVTLVDDGSK
                     DRSAAVLRQMAADDPEHVRAVILNRNYGQTSAITAGIDHSDGDIVILLDADLQNDPAD
                     IPLLLEELDKGYDVVSGWRKDRQDNKLTRTIPSRIANGLISSVTGVHLHDYGCTLKAY
                     RREILDKINLYGEMHRFIPVYAAEAGGKISEVVVHHHPRIHGKANYGLERTFKVILDL
                     ITIKFLLSYAKKPIYLFGGGGLSLMAGSFVVLLWLAIRRIAVGTPVLGSPWFQISVMS
                     FILGFISILLGLIAELLMRTYYESQDKRTYDIREIIAGGKQQEK"
     gene            9330..10373
                     /gene="ruvB"
                     /locus_tag="PKL60_05835"
     CDS             9330..10373
                     /gene="ruvB"
                     /locus_tag="PKL60_05835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012914214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNW95689.1"
                     /translation="MSAKDNDSILAGRRQPDDPADIALRPTSLGELIGQEQVKQNLQI
                     QISAAKQRGEALEHVLFYGPPGLGKTTLAHILASEMGVHIKVTSGPAVERAGDLAAIL
                     TNLHAGDILFIDEVHRLGRNVEEILYPAMEDFALDIIIGKGPAAKSVRLKLPRFTVVG
                     ATTRLALLTAPLRARFGAVYRLDYYDEAALAQIIRRGADLLHVPFDQDGILEIARRSR
                     GTPRIALRILRRVRDFAEVKADGCITADVAVESLEMMNIDGMGLDELDRRVLLCIIEK
                     YSGGPVGLNTVAASVSEEADTINDVVEPYLMQLGFLERTGQGRVATPLAHAHLGKPYN
                     GSREQPTLFNQGG"
     gene            10389..11408
                     /gene="queA"
                     /locus_tag="PKL60_05840"
     CDS             10389..11408
                     /gene="queA"
                     /locus_tag="PKL60_05840"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNW95690.1"
                     /translation="MRTDDFDYLLPPEFIAQTPVEPRDHSRLLVLERSSGRIRHDAFY
                     NIGSYLNAGDLLVVNRTRVIPARLFARKIPGGGRVEVLLLRRIDDRNWECLVGGKGLR
                     EGALIRIENGPGARVAAVLEGSKRVVCFDEAIEPFYASAGHVPLPPYIHNELVDPERY
                     QTVYSQTPGSAAAPTAGLHFTRELLAELAAAGIQIAEITLHVGLDTFAPVTEADPGTH
                     KIHQEWCELSAETAGRINAVKADGGKVVAVGTTSVRTLESAAANSGDGRVAAFCGQTG
                     LFILPGYPFRAVDAMITNFHLPKSTLIMLVSAFAGREQTLHVYEIAKNEGYRFYSFGD
                     AMLIL"
BASE COUNT         2221 a         3230 c         3426 g         2567 t
ORIGIN      
        1 atcaggaatt cctcttccag atgaatcagc agaaacttac cccgacgctc aatcccgcgg
       61 acggtcttcc cggaaattcg ctcggcaagc tcccgggggg atggggacgc gatgcttctc
      121 tcccacagga tagaaacaga cgccgcgcgc tgccccagca aaggcggcgt ggacgcatcc
      181 cccgtaagca gtccccggcg aatggtttca acctccggga attctggcac agcgtgaggt
      241 cctggtttga gctcttactc gttaaacatc gcgcctacgc tggagccttc gttggcgcgc
      301 ttaatgacct cgccaagcag ggatccgatg ctcaggattg tgagccggtc gccgaaaagc
      361 gcttttgcct cagcagatat cgggatggta ttggtggtga tgaactgcct gacaggcagg
      421 ttggacagcc tttccaccgc gttggcggaa agcaccggat gaacaaaaac cacgtatatt
      481 tctccggcgc cgtactgctt ggccagattg acggcttcgc agatggaacc gccggtatcg
      541 atttcgtcat caatcaggat gacatcccgg ccggaaacct ggccaatgat ggaaagggct
      601 tcggtatggt cctcgttatc cacgcggcgt ttttcaatga atgccagcgg aatattcagg
      661 gcttgggcgt agcgccgccc gcgcttggcg aagccgaggt cagctgtcag catgaccggc
      721 cgattcatgt tgggcagcaa gtccatgagg tagggcttga tgatgtgata accggagagg
      781 acgtccccag ggatggaaaa gaaaccctgg atttggccgg catgcaggtc catcgtgatg
      841 tagcggtcag cgccggcgac ctggatcata tccgccacca ggcgcgccgt aatcggcacg
      901 cgcggctggt ctttgcggtc ggagcggccg taacacatgt aagggacgac cacggtaatg
      961 cggccggcgg agtccagacg caaggtttgg gtcatgatga gcagttccat gagattgcgg
     1021 tgcacgggga tggaagtcgt ctgaataata tagcagtcct gaccgcggac ggatgcgtgc
     1081 agcttcacga acaaattttc gttggtgaat tcgatgacat ctcttccgca tagtggggtt
     1141 ccgaggtagt ccgaaatttc ctgcgcgagt tccggggtag acgtgcctgc aaacagacga
     1201 atatttccat acaaaaggtc gtgccgcata ttcggggaag tcatttattc acctttcact
     1261 aagtattgcg aatcccattc ctgacgcaga atgctcatca ggattatgtc gtgatactta
     1321 cctgcctgat aatggccttc ccggtagatt ccctcccggc ggaaacctgc cttttcatac
     1381 gcccgcatgc cgcgctgatt gttcgcgtaa acttgcagcc agatgcggtg caggttcagc
     1441 gtctcaaaac cgtggcgcag catcgtgcgc atggtttccg tgccgtaacc tttgttccac
     1501 caggactttt cacccagcat gataccgatt tccgcgcagc ggtcaatttc cagaatggaa
     1561 atgaaggacg tattcccgat cggcagccac tgggcttcag cgccggcttc tgacggtggg
     1621 attcttgctt caatcacgta gacatgttcg gcagccgggc gtgcaatcat gttttcaaac
     1681 cagatttctt cttccgccaa agacatcggc agccgcagaa gcaaatgctc ggtgacttcc
     1741 gggtcgctaa tccagcggat gaacataggg atatccgctt tttccgccgc gcgcaggcgt
     1801 aatctttcgc tggtaatcga gggcatcgtg aactcctatg gttccaggcg gttaggacct
     1861 ctgaacagat agacagcttc gcgaatgttg ggcaaatcca acataaccat cagcaggcgg
     1921 ctgagaccca gcccaagccc gccgtgggga gggcagccgt agcggaagta attcaggtag
     1981 ttctggatcg gaggcaaagt cagacccttg tccaaggcct gattcttcag cacttcgtag
     2041 cggtgctcgc gctgcgctcc ggtggtaatt tcaagtccgc gcccgagcaa gtcaaaactt
     2101 ttggtcagcg cggggttgtc ttcgtagacc atatggtaaa acggtcggac agagaagggc
     2161 caatctttga cgaagacgaa gtcgctgccg gttttttctt taatgtaagc gcccagggcg
     2221 cgctcagcgc cggggtccag gtcgcccttg cgttccggag ggagcttgta gccctggtcc
     2281 ttgagaatct ggtaggcttc cgccatggtc aggcgcggaa aaggaacttc gggcacctgc
     2341 aggtcgactc cgaacacgtt ttttattgct tcgccgtgct tctcctgcac gcgtttgtac
     2401 gtgtgcgcca gccagcgttc ggagaaggac atcacatcct cgtggctctc aatccaggaa
     2461 atttcaaagt ccacgccggt gaattcggtc atgtggcggg aagtaaatga tgggtcggcg
     2521 cgaaaaaccg gaccaatctc gaagaccttc tcgtagccgg ctgccatggc catctgcttg
     2581 tagaactgag gcgactgcgc gaggtaggcg gtcgtctcga aatacttcag gctgaagagt
     2641 tcagcgccgc tttcgctggg cgcgcccata agcttgggag aatgcatttc gatgaagccg
     2701 ttctccagcc aatattcgcg catggcggct tcgagggtgg tttctacttc aaaaatcagg
     2761 cggttgcgct cgcgccgcag gtccagaaaa cgccaatcca agcggtaatc ctgatccggc
     2821 agcgtctccg cgaaggggtc aaagggcagg ggattctccg cgttgttctc gaccgtcagg
     2881 ctttcgagct gaacttcaat cccgcccagt ttgacgactt cgttttccac gaccttacct
     2941 gtcacgctca gtgcggcttc tgtgctgagg gtggaaacca aagctgccag ctcagggctg
     3001 ccttttttcc agttggttat ctggacgaga cccgtccggt cacgcagaat gagaaactgc
     3061 atgctcttct ggtcgcgtaa ggtttgcagc cagccttgca gcttgacgac ttctccgata
     3121 tgcgctttca ggtctctgat cggtgttttt tccatgaatt tgctccaata aaaacaaagt
     3181 ttccttatta tgaattatac gccaggagcg cgcgcgccgc ggcacgcggc gacattttcc
     3241 gtgcgctgac ggcttgcagc gtggtttcat aaagccctga cggcagcttc ttcttccagt
     3301 ttccgataag ctcatcggta attaatttgt cgatggtctg ttggatggcg ctgctttctt
     3361 tggtatgcca ggagccagac gccttcgagt atgcccggtg ccgctggatg gcctgttcca
     3421 gctcggggat accctgcgcg gtaatcgcgg tcgttctcag gacgggcggc agccagcctg
     3481 catggtcggg cgtctgtgct tccggcgctt cggagagccg atggtgcccg ccgttaatgc
     3541 cgccggaacc gcggtagccc atttctatca tggagacgag attgcggtag gtctggtccg
     3601 cgccgggtag ttccgctttg ttcacgacca ggatgtcagc gatttccagg atgccggctt
     3661 tgatggtctg gatgtcatcc cccatgccgg gcgtatccac cacgatggtc gtatgggcga
     3721 ggttgacgat atccacttcc gcctgccccg cgccgaccgt ttcgataagc acagtatcat
     3781 atccggcggc gtcgaacacc tggctgagcg caagcgtggc ttcggccagg ccgcccagcg
     3841 cgccgcgcgt ggccatggag cggataaaga ccccggtgtc gttctgcaag tccggcatgc
     3901 gcacgcggtc tcccaggatg gcgccgccgg tgaaggggct ggtcgggtcc accgcgataa
     3961 tcgcgacgcg cgaatccggg aaagtacggc gcaggtagtg cgccagcgcg ttcaccaggc
     4021 tgcttttccc ggagcccggc gcgccggtga cgccaatttt gtgcgcgtgg cctgtaaagg
     4081 caaacagcgc gtccaaggca gcgcgtccgt cttcgtcatc cgcttccacg cggcttaata
     4141 cacgagaaag ggcaagttta tcgccctttc tgacagcttc cacggaaaaa tcggtcatgg
     4201 ttcaggcgcg ctctgccagc gccgcgcgaa tatcctggca gacgacacgg gtatccgtgc
     4261 cgggtccgta cactttggtg acgccctcag cgagcaggcc gggaatatcg cgttccggga
     4321 tgataccgcc caggaaaacg aagacgtcat cctggccgtt ttctttcagt ttctcaagaa
     4381 cctgggggac gagagcgttg tgcgcgccgg agagaatgga aagcccgacc acgtccacgt
     4441 cttcctgcag ggcggcttcg gcaatcatct ggggagtctg gcgcagcccg gtgtagataa
     4501 cttccattcc gtcgtcccgc agcgcccggg caatcacctt cgcgccgcgg tcgtgaccgt
     4561 ccaggccggg tttggcgata agcacacgta tcttcttttc aggcatccct ttacctccgc
     4621 gttgatactg acattatact gccaaaaagg cttgcgaccg ggctggttaa tcagcagggc
     4681 gggctgccgc gcttcaggct gcgagaaacc cgggctcagc gcgaacagcc accatttccc
     4741 cgtagtacat acggtggaag tctttcagcg gataatggcg cccgatgctg gggtccaaaa
     4801 agcagcttgg atccaggtcc gcccggtaaa tctttttgca ctcaatcacc aattccgcct
     4861 cggcaaagct gggcgcgctg accgcctggg aagcctgcgg ggtgagcctg gtgcggctga
     4921 gtttatcttc attccggccg gagtgcgtgc ccaggaaaag cacgtcctct ttgtatgcct
     4981 ccggaaaggc gcagagcgta aagtccggcg cgttctcgat gaagttgtag gtgtagcgcg
     5041 tggggcggac cggcacaatc acgcagggtc tgttccacat gcagccgaga aacccccagc
     5101 caatgaccat cgcgttgaag ttccgctgag cgaagctgcc gctggtgagg acaagtgtct
     5161 gacgctcaat caggtcatag ggttttacga ggagttcttt tggcgagatt gtctgaaggg
     5221 tcatgttttc ctccgctgtg gggctggttg gcgcaatttt atcgcatttg gcgggcagca
     5281 ccccggctgc agtataatac tcgcggaatt aaacccacat cgctatgaaa aaagacacga
     5341 aaatcaactg gaacctcatc atccgctggg ctggcacgct tttgtcgatc ggcatcctga
     5401 tttacctgtt caggcagatt ggggcggagg aaacctgggc ggctgtgcaa aaaatcagcg
     5461 ggcggcggat tgcggaggtg ctggtattgg tgttcgtctc gcgcttggcg acctggggcc
     5521 gctggcacac gcttttacag gtgcaagcgc tgcgcgtgcg ctggcaggac agtttgcgcc
     5581 tgaccttcgc gggtttgttt gcttctaatg tgctgccgac caccatcggc ggcgacgttg
     5641 tgcgtttggc cggggcggtg cggctcggaa tcgacgctgc gctggcggcg gcttcactgg
     5701 ttgccgaccg tttggtgggt atgaccggta tggcgctggc gctgcccttc gccatccccg
     5761 cgctttcaag ctacttgaag gctcaggctt cctccggcgc agctcaaacc accggggcgg
     5821 cggctttgtt ccccgctttt ttccggaaaa tccagcaagc cataaaaaaa gttttctcga
     5881 attttcgctt ttggctcaag cacccgctta tcctggctca ggcgttgggc ttcaccttca
     5941 tccacatgct ggcgaccttt ctcatcgtcc aaattctggt gcagggaatg ggcgaaagta
     6001 tctctttcac aaagacagcc gggctgtgga gcctgaccta tttcatttcg ctaatcccgg
     6061 tctcgattaa tgggctcggg ctgcaggaac tgaccattac caacctgttc tcggctctgg
     6121 gcggactgca gccatccacc agcatcagcc tggctgtcat cctgcgcgtg gtttggatga
     6181 ttggcagcct gcctggcgct ttcttcatcg gcggcatcct ggggggcaaa cgtccttctg
     6241 aagcaggagg ggaaaaacaa aatggaaaca cgcaggatta aggctttcct gaaccagttc
     6301 ctggcggtca gcctgggttt ggcggcgata ttcctggtcc agaatcgggc tgaacgcggg
     6361 cgatcttact gggcgggcag ccgcgcttat gccggtgttg gcgcttgggg ttttcctgat
     6421 gcagcggctc atccagactc ctgagaagtt aaagcacaaa gttccgcgct ggttggcgta
     6481 cggcatcagc gcgctgctgc aaattggttt gctggcagtt ttgctcacgc aggactttca
     6541 gcttatccat tccacgattg gcaggctgtt tttgtcagtc ctggcagctg gcggtgcggc
     6601 gctgctgttt ctcacgacgg aaaagcccgg ctccccacag cgcagcttca tcaccgcgct
     6661 gctggttttc ggagcgctct accgcgcagc cgccttcctg cctgagcttc aaccgggtcc
     6721 gttcgccttg ggttggtccg agggcagccg cttttacaac gcctcattgt tcttgtccgg
     6781 gcgcatttat gggcagaaac tgccgctgcc ggttttgcat ccttcccgct acctgctgca
     6841 agcctttcct ttcctgcttg ggatcaagtc catcctggcg caccgcgtat ggcaggtact
     6901 gctctggctt gggatgagcg cctggggggc agccctcttc gccaggcgtc tgggaaagga
     6961 tcaaggcatc tcgttcggcc tgctgacgat ttgggcagct ttgttttttt tccagggcgc
     7021 ggtttattac cacctgatgg gttgcgtcat cctggtgctg cttggctacc gcaaagacaa
     7081 aatctggcgc acacttgctt ttgtaattct ggcttcgctc tgggcgggca tcagccgcgt
     7141 gaactggatg ccgttaccag gcttgctggc aggcgcgctg tatctgttgg atgaaccgct
     7201 cgggcagaaa aaatggctga aatatctcgc gccggttctt ttgtgggctg tgttgggctt
     7261 cgcttccgcc tggctggcgc agcagggcta tatccgcatt tccggggagg accccgcgct
     7321 gtttaattcc gccttcagtt ccgcgctcat ctggaaccgc ttgctgccaa acccgacttt
     7381 cgggctgggc attcttccgg caattctgtt ggtttgtttg cccgggatgg gcttgctggc
     7441 tcagcgcgtg tttgccaggc aagcgccggc tctgcattgg ctgcgctggc tggggctggc
     7501 ggggattttg ggagcgttct tcctgggcgg cctgttggtc agcctgaaaa ttggcggcgg
     7561 gggagacctg cacaatctgg acggcttcct ggtcttttgg gtgctaatcg caggtagttt
     7621 gctggcaggc gcttatgggg cggaaacccc tcaaaacgcg ccggctccag gcataaggcc
     7681 tgctttttcc tggttgattc tcgcggcatt gattccgctc tacttcgcgc tttcacactc
     7741 tgctgtgtgg ggcttcacac ccgcggccga ccaacgggtg gaagtggcgc gcatgcaggc
     7801 tgccctggac ctgttgaaag acgaacctgg cgaggtgtta ttcatttccg aacgccagct
     7861 gctcagtttt aacgaaatcc agggcgtgcg cgtggtgcct gagtatgaaa aagttttcct
     7921 gatggaaatg gcgatgggca acaaccagcc ttacctgcag gctttttacg ccgatttgac
     7981 aggtcggcgt tttaaggcga ttatcaccga cacgatttcg acccgcatcc aaggggacgg
     8041 caagtctttt ggcgtggaaa acgatgcctg ggtacaaaag gttttggagc cgatgctgca
     8101 aacctacgaa ccagtgctga gctggaacaa cgccagcgcg cacctgctga tacctcaggg
     8161 gcaggacgcg ctcaaacagg cgctgctcga actgcagccg taaacctttc tacttctcct
     8221 gctgcttgcc cccggcgatg atttcgcgga tatcgtaggt gcgtttgtct tgcgattcgt
     8281 agtaggtgcg catcagcagc tcggcaatca aacccagcag gatggaaatg aatccaagga
     8341 tgaaggacat cacgctgatt tgaaaccagg gcgaaccgag cactggcgtt ccgaccgcga
     8401 tgcggcgaat tgccagccat aacagcacga caaagctgcc tgccatcaga ctcaggccgc
     8461 cgccgccgaa cagataaatc ggcttcttgg cgtagctgag cagaaatttt atggtgatta
     8521 agtccaggat gaccttaaaa gtgcgctcca gaccgtaatt ggctttgccg tggatgcgcg
     8581 ggtggtggtg cacgaccacc tcgctgattt ttccgccggc ttccgcagcg tacaccggga
     8641 taaagcggtg catttcgccg taaaggttga ttttatccag gatttcgcgc cggtacgcct
     8701 tgagcgtgca gccataatca tgcaggtgca cgccggtcac gctggaaatc aggccgttgg
     8761 caattctgga ggggatggtg cgggtaagtt tgttgtcctg ccggtctttg cgccagccgc
     8821 tgaccacgtc ataacctttg tccagctctt ccagcagcag cgggatatcg gcggggtcgt
     8881 tttgcaggtc ggcatccagc aggatgacaa tatctccgtc cgaatggtca atgccggcgg
     8941 taatggcgga ggtctgtccg tagttgcggt tgagaatgac cgcgcgcacg tgctccgggt
     9001 cgtccgcagc catctgcctc aggaccgcgg cgctcctgtc cttgctgccg tcgtccacca
     9061 aggtgacgtc ccagtccatt tccagcggcg caaccgcctc gtaaatgcgc gggagcagtt
     9121 cgggcaggtt ttcctcctcg ttatacacgg gcacgaccag cgataatttc attcagcctc
     9181 caaagcaggg gaagacccca gcatatcatc tattttccca attatactca tccttgccgt
     9241 acccgcatcc agcgcagaca gcgaccatcc ggagctaaag ccgcctgtcc gggcagcttt
     9301 gagagcagaa aacgggtata attccggcaa tgagcgcgaa agataatgac agcatactcg
     9361 cggggcgcag acagccggat gaccctgctg atatcgcact gcgtccgacg agcctggggg
     9421 agctgattgg tcaggaacag gtgaaacaaa acctgcaaat ccagattagc gccgccaaac
     9481 agcgcggcga agccttggag cacgtcttgt tttacggccc gcccgggctc gggaaaacca
     9541 cgctagcgca tattctggcc tcagaaatgg gcgttcatat caaagtgact tccggccctg
     9601 cggtggaacg cgctggcgac ctggcggcta tcctgaccaa cctgcacgcc ggcgatatcc
     9661 tcttcatcga cgaagtgcac cgcctggggc gcaacgtgga agaaatcctt tacccggcca
     9721 tggaagactt cgcgctggat atcatcatcg ggaaaggtcc cgcggccaaa tccgtgcgcc
     9781 tgaagctgcc gcgcttcacc gtggtcggcg caactacgcg tctggcgctg ctgaccgctc
     9841 cgctgcgcgc gcgttttggc gcggtttacc gtctggacta ttatgacgag gcagcgctcg
     9901 cgcaaattat ccggcgcggc gcggacctgc tgcatgtgcc cttcgaccag gacggcatcc
     9961 tggaaatcgc gcgccgctcg cgcgggacac cgagaatcgc gctgcgcatc ctgcgtcgcg
    10021 tgcgggattt tgcggaggtc aaagctgacg gctgcatcac agcagatgtt gcggtggaga
    10081 gcctggaaat gatgaacatc gacgggatgg gtctggacga gctggaccgg cgcgtattgc
    10141 tgtgcatcat tgagaaatac agcggggggc cggtagggtt gaacactgtg gcggcttcgg
    10201 tcagcgaaga agccgatacg atcaatgatg tggtggagcc ctaccttatg cagctcggtt
    10261 tcctggaacg cacggggcag ggcagggtgg caactccgct ggcgcacgcc catcttggca
    10321 agccctacaa cggttcccgc gagcagccga ccctgtttaa tcagggcggc tgatttccct
    10381 ggtagaaaat gagaactgac gattttgact atcttctgcc gcctgagttc atcgctcaga
    10441 cgccggttga accgcgcgac cattcgcgct tgctggtgct ggaacgctcg tccggaagga
    10501 tccgccacga cgccttctat aacatcggca gctacctgaa tgccggcgac ttgctggtcg
    10561 taaaccgcac ccgggtcatc ccggcgcggc tgtttgcccg gaaaatcccg ggcggcgggc
    10621 gggtggaggt gctgctgctg cgccggattg atgatcgaaa ctgggaatgc cttgtggggg
    10681 gcaaaggctt gcgcgaaggc gcgctcatcc gcatcgagaa tggaccgggc gcgcgcgtgg
    10741 ccgcggtatt ggaaggctcc aagcgcgtgg tctgttttga cgaggcgata gagccttttt
    10801 acgcatcggc ggggcatgta ccgctgccgc cctacattca caatgagttg gtggacccgg
    10861 aacgctatca gactgtctac agccagacgc ccggttcggc ggcagcgcct accgccgggc
    10921 tgcacttcac ccgggaattg cttgcggagc ttgccgcagc cggcatacaa atcgcggaaa
    10981 tcaccctgca cgtcgggttg gataccttcg cgccggtgac ggaagcagac cccggcacac
    11041 ataaaatcca ccaggaatgg tgtgaacttt cagcggaaac cgccgggaga atcaacgcag
    11101 tgaaggcaga cgggggaaaa gtggtcgcgg tcggaacgac ttcagtgcgc actttggaga
    11161 gcgcggcggc aaactcgggg gatggcaggg tagcagcgtt ttgtggtcaa accggattgt
    11221 tcatcctacc tggctaccca ttcagggcgg tggacgcgat gattaccaat tttcatctgc
    11281 ctaaatccac gctcatcatg ctcgtcagcg cttttgccgg gcgcgagcaa acccttcatg
    11341 tttatgaaat cgcgaaaaat gagggctatc gcttttattc tttcggcgac gcgatgctca
    11401 tcctctaacg ttaattcaat tcttacgcct ggcggttaaa ctat
//
LOCUS       DAOQAD010000058        11310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_6561_length_11310_cov_7.345624, whole genome shotgun sequence.
ACCESSION   DAOQAD010000058 DAOQAD010000000
VERSION     DAOQAD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11310
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6561_length_11310_cov_7.345624"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..1161)
                     /locus_tag="PKL60_05845"
     CDS             complement(241..1161)
                     /locus_tag="PKL60_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNW95691.1"
                     /translation="MAKVVAVNISAETGVIKTTVESAVLVEKFGIEGDAHAGNWHRQV
                     SLLDQASVDKMTALGAEGLKPGIFAENITTEGIALHTLPVGTRLRVGEAELEVTQIGK
                     ECHAHCQIYQQVGMCIMPTEGIFTVVIRGGEIRGGDPIEILNGVRAAVITISDKGAAG
                     LREDKSGPALVEALHGAALVTEQLIIPDEIELIKAELTRLADGGSLDVIFTTGGTGLA
                     PRDVTPEATLAVVERLVPGIPEAMRAESRKITDRAMLSRAQAGVRGHTLIINLPGSPK
                     AAVECLQVFLPALAHAVETLRGEAYECARP"
     gene            complement(1164..2111)
                     /gene="moaA"
                     /locus_tag="PKL60_05850"
     CDS             complement(1164..2111)
                     /gene="moaA"
                     /locus_tag="PKL60_05850"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNW95692.1"
                     /translation="MIDQYQREITYLRLSLTERCQLRCTYCRAEEGACPKAEELSAAD
                     FARIVRVMARLGINKVRLTGGEPLLRKDVLDIVSRVAAAAELKDLSMTTNAQMLPGKA
                     AALKAAGLRRLNISLDSLRPDVFREMTGGDLRLVLNGIDEALEAGLVPLKINIVLVAG
                     MNGGEVDDFIALTRERPLDVRFIELMPIGDQGRNDGRRISNQDLLRARPYLQALPPRY
                     PGQPSTDYQVPGYLGRVGFISPISHRFCEDCNRIRVMSDGMLRPCLGVDRQISLKNAL
                     RGGDDELLELIRQVIFEKPIGHAFQSCSASERDMSRIGG"
     gene            complement(2104..2595)
                     /gene="moaC"
                     /locus_tag="PKL60_05855"
     CDS             complement(2104..2595)
                     /gene="moaC"
                     /locus_tag="PKL60_05855"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNW95693.1"
                     /translation="MELSHLDKNGHARMVDVSAKEDTAREALASALVRMLPQTLALIQ
                     TGEMPKGDVFACARVAGILAAKRTPELIPLCHPIPITLVSVDFEVMGQDTLRVTARVK
                     TSSRTGVEMEALTAVSVAALTVYDMCKAVDRGMQILQIQLEQKSGGKSGDFLREDIIP
                     AND"
     gene            complement(2586..4502)
                     /locus_tag="PKL60_05860"
     CDS             complement(2586..4502)
                     /locus_tag="PKL60_05860"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HNW95694.1"
                     /translation="MAFEYLTNIPLEEAREMYLSALRQAGLSYKTEIIPTNSALGRVS
                     AHAVYARISSPHYNACAMDGIALDAKRTFGASETTPARLNADDFVWVNTGDPLPEGCD
                     AVVMVEDVVQEGDGAVLYAAALPWQHVRQIGEDISAGDMILPSFSVLSPAALGALLES
                     GVLNVEVAARPRAGIIPTGNELVAPTEDPQAGSIIESNSAVFAGMLETWGCVPKIYPG
                     VRDDPALIEAALRTALAECDLVVLNAGSSAGRKDFSTDAIRQVGEVVAHGVAIKPGKP
                     AILAHARTDSGQVVPVLGLPGYPVSGILVMEELFKPVVESLTCRPSQKPDFIQAAISR
                     RLTSSLKYLEFVRTRLGNVNGKTVAVPLNRGAGVVSSFVKADGIIHIPQNTEGYEAGE
                     LVNVQLTRSRAEIDRTLVITGSHDPLLDEVADILKRLDRDSSVASSHVGSMGGITAVK
                     RGEAHLGGIHLLDEASGTYNIPYLRKYFPQGGVVLVECVQRTQGLMVPKGNPLGIREF
                     ADIAGKRYVNRQKGSGTRILCDFLARQNAIDADSVWGYEREEYTHTAVAAAIAAGTAD
                     AGLGILSAARIYNLDFVPVCEEQYDLLVAESAFHLEMVQRLLEVLRGKEFARRLAVMG
                     GYSLKTPGEIRPWN"
     gene            complement(4505..5713)
                     /locus_tag="PKL60_05865"
     CDS             complement(4505..5713)
                     /locus_tag="PKL60_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNW95695.1"
                     /translation="MLTVITIAEAQRLIARSFGSLKMPAEAVCLAEAGGRALAQDVLA
                     GEFVPDFDRATVDGYAVRSRDVFGCSESIPALLNKIGAAQMGAHTDLNLAAGECVYVP
                     TGAEIPAGADCVIMLEDVEVLEDGLVAVYKASPPGANLIFRGDDLRPGEVVLRAGTRL
                     SPAHVGALAAMGLNEVPVRRFPRAAVISTGDELVPAGEALPPGKIRDVNAPMLLQAVQ
                     DAGGRADFLGIIPDRIEAVRAALISALVGHDLVLISGGTSVGEKDAVPLVLAELGELL
                     AHGLAVKPGKPTLFGSAGGKPVFGLPGNPVAAFFMFHLLARPLMASMLGTELDERRVW
                     APVARAVPSNHGREEYVPVMLQEGQAHPIPSKSGLITTLARADGFLAIPRDKEGLPAG
                     ELVEVTLLKR"
     gene            complement(5799..6719)
                     /locus_tag="PKL60_05870"
     CDS             complement(5799..6719)
                     /locus_tag="PKL60_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95696.1"
                     /translation="MEHESAAQAYELYHDESARNGYWHGMFLVPTRQKEALAAYLEAA
                     RAEVGYPHKISFKKINRPGAKRDLAEAWLSIALGFMRSESKHIRYFYYSGARRERERA
                     FRVLDDQAGGAKFILFRERDNHEDMLHYPDETSKVETSFRFGFKGGLHYLFSPENPVC
                     ITKIHFDGYLHHGRHIDRQRVVERLSGLRSYCGVGSQPDLIDDRASDPAMQDAQPYAD
                     CELLQLTDLLIGSFRAAFGYYSNPSQWLLARYARTLIDKYRQGPPRMRKSRWNRAFCM
                     SQCYLEDGAWRFETINLMEDEGVSQLPLLE"
     gene            6932..8554
                     /locus_tag="PKL60_05875"
     CDS             6932..8554
                     /locus_tag="PKL60_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNW95697.1"
                     /translation="MKTVIAGALGECVHVAGVMNFLNLAEIAGWRTVFLGPAVPVEEF
                     LRAAREEGADLVGASYRLTPETGERLLGEFAEAADELRAQGVRFVFGGTPPVAQRARM
                     LGFFERAFDGSEAPEEVLAFLRGESAEPGSPTYYPQTTLERIAWKTPYPLLRHHFGLP
                     TMAATLEGIAQIAEARALDVISLGTDQDAQENFFHPERQNPRAKGAGGVPVRSAEDYR
                     ALFRASRRGNYPLLRTYSGTDDFIRLAELYMETINNAWCAVPLFWFNQLDGRGPVSLE
                     DSIRLHQELIRWYGQRGVPVELNEPHHWGMRDAPDVIFLVSAYLSAYNAKVFGARDYI
                     AQMMFNSPPGASDAMDLAKMLAILEMSAALEDENFRIFRQTRTGLLSYPLESNAARAH
                     LSASIYLQMALQPHIVHIVGHTEADHAATAADVIEASLMARQAVANALKGQPDMLADP
                     KVQARKEHLITEARLTLTAISNLGQLGSDPLSDPAVLGKAVRLGILDAPHLRGSKIAR
                     GEIRTRVIQGGCEAVRSDGSPLPEAERLSTYL"
     gene            8568..9671
                     /locus_tag="PKL60_05880"
     CDS             8568..9671
                     /locus_tag="PKL60_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HNW95698.1"
                     /translation="MNDNLKYTVIGAGNGGKAMAAHMALMGKEVTLFNRTFANIEVIA
                     ERGGIDLENPGGISGFGRLAKVTDKIGEAVADADVIMVVVPSSGHRDIAAAAAPYLRD
                     GQIVLLHPGRTCGAIEFVEVLRQSGCKTKPIVAEAETFIYASRSEGPSHAKIFRIKES
                     VPLAALPATKNRAVLDAIHHVYPQYIDGGNVLKTGLNNMGAVFHPTITLLNAGWIETT
                     HGDFEFYIDGVSPSVAKLLETVDRERCTVASALGIRARNGLEWLALAYDARGENLHEA
                     IHNQSGYYGIKAPPTLNHRYLFEEIPMSLVPIAALGQRYGVSVRGIDAIIRLACIIHN
                     TDYWRKGRTLDKLGIQDLSIEELTHYVETGERE"
     gene            9917..11062
                     /locus_tag="PKL60_05885"
     CDS             9917..11062
                     /locus_tag="PKL60_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNW95699.1"
                     /translation="MNTVQVGTVRLLPAGYQAFIPTIFPPKAMLDFPKDILMKNSDAT
                     LKVGELKGITRLLPDIDFFLWMYLRKDAASSSQIEGTVATMIDAIEAEANINSDLPED
                     VDDILHYLTALHFGIQQIEDGKLPLSLRLIKDLHYQLLHEARTSHLANPGEFRTSQNW
                     IGGTTLNNARFVPPPPAYLSNALGDLEIFMHAEDAIPMLLKAGLLHAQFETIHPFLDG
                     NGRTGRLLITLFLWNKHYLEKPVLYLSSYFHAHRQEYYDRLEGYHQGKVFEWLDFYLD
                     GIMQTATEAIETVGKITALREKDMRLIQALGKRSAELITKLLPRLFAQPIINVQTIQD
                     WTGYTRDGAYKMVTRLVGLGILVPRFTDKTYGQSFMYKNYLDIFTES"
BASE COUNT         2301 a         3482 c         3164 g         2363 t
ORIGIN      
        1 gtcgggttgc gctcctgcgt cgctgcaccc gacctacagc tgcacccgac ctacagctgc
       61 acccgacctc cagcattcgt catcgcgagg aacgaagcga tctccccgtc agcctcttgg
      121 gagattgctt cagtcgctgg ctccttcgca atgacgaagt acgcaatgtc gggttgcgct
      181 cctgcgccgc cgcacacacc ctagctcttt ttcaacctga acccgaccct caagtccctt
      241 tcacggtctg gcgcactcgt aggcttcgcc gcgcagagtt tccaccgcgt gcgcaagcgc
      301 cggcagaaat acctgcaggc actccaccgc cgccttgggg ctgccgggca ggttgataat
      361 cagcgtgtgc ccgcgcaccc ccgcctgtgc tctggaaagc atcgcccggt cggtgatctt
      421 gcggctctcc gcgcgcattg cctccgggat gcccggcacg agcctttcca ccaccgccag
      481 ggtggcttcg ggtgtcacat cccgcggcgc caatccggtc ccgccggtgg taaaaattac
      541 atccaggctg cccccatccg ccagacgcgt caattctgct ttgatcagct caatttcgtc
      601 cgggataatg agttgctcgg ttacaagcgc tgccccgtgt aaagcctcca ccaaagccgg
      661 cccgctctta tcctcgcgca agcccgccgc gcctttgtcg ctgatggtga taaccgctgc
      721 gcgcactccg ttcaggattt caatcggatc tccaccccgg atttcccctc cgcggatgac
      781 caccgtaaag attccctccg tcggcataat gcacatgccc acctgctggt aaatctggca
      841 gtgcgcatgg cactccttgc caatctgcgt gacttccagc tctgcttccc ctacgcgcag
      901 gcgcgtgcca accggcagcg tgtgcagcgc aatgccttcg gtggtgatat tttcggcaaa
      961 aatgcccggc ttcagccctt ccgccccaag cgcggtcatc ttgtccacgc tggcctggtc
     1021 caacaggctc acctggcggt gccagttacc ggcatgcgcg tctccctcta tcccaaactt
     1081 ttccaccaga acagcgcttt ccaccgtcgt cttaatgacg cctgtctccg cgctgatatt
     1141 caccgcgaca acttttgcca aacttatcct cctatcctgc tcatgtcccg ttcggaagcc
     1201 gaacaacttt gaaacgcgtg accaataggt ttctcaaata tcacctggcg gatgagctcc
     1261 agaagttcat cgtcaccccc gcgcagggcg tttttcaagg aaatctgacg gtccaccccc
     1321 aggcaggggc gcagcatacc gtcgctcatc acgcggatgc ggttgcagtc ctcgcaaaac
     1381 cgatgcgaga ttgggctgat aaagcctacc cgacccaggt agccgggcac ttgataatcc
     1441 gttgaaggct gcccggggta acgcgggggc agcgcttgca ggtacggacg cgcccgcagc
     1501 aaatcctggt tggagatgcg ccgcccgtca tttctgccct ggtcgccaat gggcatgagc
     1561 tcgataaacc gcacgtccag cggtcgttcg cgcgtgagcg cgatgaagtc atcaacctcc
     1621 ccgccgttca tgcccgccac gaggacgata ttaatcttca gcggcacaag ccccgcttcg
     1681 agcgcttcgt caatgccgtt cagcaccagc cgcaaatccc cgccggtcat ttcccggaac
     1741 acgtccggcc ggagcgagtc caggctgata ttcagccggc gtaagccggc agccttgagg
     1801 gcggccgcct tgcccggcag catctgggcg ttggtggtca tagaaaggtc tttcagttcc
     1861 gccgccgcag ccacccggga aacgatatcc agaacgtctt tgcgcagcag gggttctccg
     1921 cctgtcaacc gcactttgtt gatccccagg cgtgccatca cgcgcacaat tcgcgcgaag
     1981 tccgcggcgc tcagctcctc tgctttgggg caggcgcctt cctccgcgcg gcagtaggtg
     2041 cagcgcagct ggcagcgttc ggtcagggaa agccgcaggt aggtgatttc ccgctgatat
     2101 tgatcaatca tttgcaggga ttatatcctc tcgcaggaaa tcaccggatt ttccgccgct
     2161 tttttgctcc aattggattt gcaggatttg catgccgcgg tccacggctt tacacatatc
     2221 gtacacggtc agcgccgcaa cgctgaccgc cgtgagcgct tccatttcca caccagtcct
     2281 ggagcttgtt tttacccggg ctgtaacgcg caacgtatcc tgccccatca cttcaaaatc
     2341 cacgctgacc aaagtaatcg ggatggggtg gcagagcggg ataagctcag gcgtgcgttt
     2401 ggcggccaat attccggcca cgcgcgcgca tgcgaaaaca tcgcccttgg gcatttcccc
     2461 ggtctggatg agcgccaggg tttgcggcag catgcgcacc agcgcgctgg caagcgcctc
     2521 gcgtgctgta tcttctttcg ccgaaacgtc caccatgcgt gcgtgtccgt ttttatccag
     2581 atggctcagt tccatggtct gatttctccc ggggtcttga gcgaataacc gcccatcacg
     2641 gcaagtctgc gcgcgaattc tttcccgcgc aaaacttcca gcaagcgctg caccatttcc
     2701 agatggaagg cgctctccgc gacgagcaaa tcgtactgtt cttcgcacac cggcacaaag
     2761 tccaggttgt agatgcgcgc ggcggacaag attccaagcc cggcatcggc tgtccctgcc
     2821 gcgatggccg ccgcgaccgc ggtgtgggta tattcctcgc gctcgtagcc ccacacgctg
     2881 tccgcatcga tggcattctg ccgcgccaaa aagtcgcaca ggatgcgcgt tccgctgcct
     2941 ttctggcggt tgacgtagcg tttcccggcg atatccgcga attcacggat acccagcgga
     3001 ttgcccttgg gaaccatcaa accctgcgtg cgttggacgc attccaccag taccacgccg
     3061 ccctgtggga agtatttgcg caaatagggg atgttgtaag tgccgctggc ctcatccagc
     3121 aggtgaatgc cgcccaggtg ggcttcaccg cgtttgaccg cggtaatccc gcccatgctg
     3181 cccacatgcg aggacgcgac agaactgtcc cgatccaggc gcttaaggat atccgcgacc
     3241 tcatccagca gcgggtcatg gctgccggtg atgaccaggg tgcggtctat ctcggcgcgg
     3301 ctgcgggtca gctgtacgtt caccagctcg ccggcctcat agccttcagt gttttggggg
     3361 atatggataa tcccatcggc ttttacgaag gaactcacca cgccggcgcc ccggttcagc
     3421 ggcaccgcca cggttttgcc gttcacgttc cccaggcgcg tgcgcacaaa ctccaggtac
     3481 ttaagcgagg aggtcagccg gcgcgagatg gcagcctgga tgaaatcggg tttttgagaa
     3541 ggccggcagg tcaggctttc cactaccggc ttgaatagtt cttccatcac caggatgccg
     3601 gaaacaggat agcccggcaa accaagcacg ggcacgacct gcccgctatc cgtccgcgcg
     3661 tgcgccaaaa tcgccggctt gccgggtttg atggccacgc cgtgcgcaac cacctcgcct
     3721 acctgacgga ttgcgtcggt ggaaaagtct tttctgcccg cggaggagcc ggcattcagt
     3781 accaccaggt cgcactccgc cagggcggtg cgtagagccg cttcaatcag cgcggggtcg
     3841 tcccgcacgc ccggatagat tttgggcaca cacccccagg tctccagcat gccagcgaag
     3901 acggcagaat tggactcgat gatgctgccg gcttgcgggt cttcagtcgg ggcgaccagc
     3961 tcattgccgg tcgggatgat tccggcacgc gggcgagccg ccacctccac attcagtacg
     4021 ccggattcca gcagcgcgcc cagcgccgcc ggggaaagca cgctgaatga cggcagaatc
     4081 atatcgccgg cgctgatatc ctcgccaatc tggcgcacgt gctgccaggg cagcgccgcc
     4141 gcgtagagca ccgcgccgtc cccttcctgc actacgtctt ccaccatcac caccgcgtcg
     4201 cagccctcgg gcagcgggtc gccggtattc acccacacaa agtcatccgc gttcaagcgc
     4261 gccggcgtgg tttcagaagc cccaaacgtg cgcttcgcgt ccagggcgat gccgtccatc
     4321 gcgcaggcat tatagtgcgg agagctgatg cgcgcgtaga ctgcgtgcgc gctcacgcgg
     4381 cccaaggcgg agttggtcgg gattatctcg gttttgtagc tcagccctgc ctgccgcagc
     4441 gcgctcaggt acatttcgcg cgcttcttcc agggggatat tggtcagata ttcgaatgcc
     4501 atatttacct cttcaacagg gttacttcca ccagctcgcc ggctggcagg ccttccttgt
     4561 cgcgcgggat ggcgagaaag ccgtccgcgc gcgccagggt ggttatcagc ccggacttgc
     4621 ttgggattgg atgcgcctgg ccctcttgca gcatcacagg cacatattct tcgcgcccgt
     4681 ggttggaggg caccgcgcgc gcgacaggcg cccacacgcg ccgttcgtcc agttccgtgc
     4741 ccagcatgga cgccatgagc ggacgcgcca gcaaatgaaa cataaagaaa gccgccaccg
     4801 ggttgcctgg caagccaaaa acgggttttc ccccggcgct gccaaacagc gtcggtttgc
     4861 ccggcttgac tgccagcccg tgcgccagca gttcgcccag ctcagccaac accagcggta
     4921 ccgcgtcctt ctccccgacg gatgtgccgc cggaaatcag caccaggtcg tgcccaacaa
     4981 gcgcggatat cagcgcggca cgcactgcct cgatgcggtc gggaataatg ccaagaaagt
     5041 ccgcccgccc gccggcatcc tgcacagcct gaagaagcat cggggcgttc acatcgcgga
     5101 ttttgcccgg cggcaaggcc tcccccgcgg gcacgagctc atcaccggtg gaaatgaccg
     5161 ctgcacgcgg aaaacgccgc acaggaacct cgttcagacc catcgctgcc agcgctccga
     5221 catgcgcggg gctcaggcgc gtacccgccc gcaggacgac ctcccccggg cgcaggtcat
     5281 ccccgcgaaa aatcaggttc gcgccgggcg gggaggcttt gtagaccgcc accaaaccgt
     5341 cctccaacac ttcgacatct tccagcataa tcacgcagtc cgccccagcc gggatttctg
     5401 cgccggtggg aacgtagacg cactcccccg ccgccagatt caggtcggtg tgcgcgccca
     5461 tctgcgcggc gccaattttg ttaagcaggg cggggatgga ctcggagcag ccgaaaacgt
     5521 ctctgctgcg cacggcgtaa ccgtccacag tcgcgcggtc gaaatccggg acaaattctc
     5581 cggccagtac atcctgcgcc agggctctgc cgcccgcctc ggccaggcaa acagcttccg
     5641 caggcatttt taagctccca aagctgcgcg cgatcaggcg ttgggcttcg gcaatcgtaa
     5701 tgacagtgag catcgcgttt tcattgctcc ggaacgtaat attttgataa tcttaccaga
     5761 tgcagcgccg gccggcgctg tgcgtacagg cgcacaggct attccaacag cgggagttgg
     5821 ctgacccctt catcttccat caggttgatg gtctcgaagc gccaggcgcc gtcttccagg
     5881 tagcattggc tcatgcagaa ggcgcggttc cagcggctct tgcgcatacg cggcggaccc
     5941 tgccggtatt tatcgattag cgttctggcg tagcgcgcca gcagccactg agaagggttg
     6001 ctgtaataac cgaatgccgc ccgaaatgag cctatcagca ggtccgtcag ctgcaggagc
     6061 tcgcagtccg cgtaaggctg cgcgtcctgc atagcggggt cgctggcgcg gtcgtcaatc
     6121 agatctggtt ggctgccgac tccgcagtag ctgcgcaagc ccgaaaggcg ttccactacg
     6181 cgttggcggt cgatgtgccg tccatggtgc aggtaaccgt caaaatgaat tttcgtgatg
     6241 cacactggat tttccgggct gaagaggtag tgtagcccgc ctttgaagcc gaagcgaaaa
     6301 gaggtttcca ctttgctggt ctcatccggg tagtgcagca tgtcttcgtg gttatcgcgt
     6361 tcgcggaaga ggatgaactt ggcgccgccc gcctggtcgt ccagcacgcg gaaggctctt
     6421 tcccgctccc tccgcgcgcc gctgtaataa aagtagcgga tgtgcttgct ttccgagcgc
     6481 atgaaaccca gggcgatgct cagccaggct tccgccaggt cgcgcttggc gcccgggcgg
     6541 ttaatcttct tgaaactgat tttgtgcggg taaccgacct ccgcacgcgc ggcttccaga
     6601 taagctgcca gggcttcctt ctggcgggtc ggaaccagga acatcccgtg ccaatacccg
     6661 tttcgggcgg actcatcatg atacagttcg tacgcttgcg cagcactttc gtgttccatt
     6721 tctgttatca ggattgtacc ccacccggtc ggaatgggac cgcgcggcat taagtttcta
     6781 cgtatttagg tttcggaaag ccttctttca atttcgggta caattaatta tcgctttctt
     6841 agctaacccg gcatgggttg tctttatctt ttctttgttc accatttggt gagtgaccga
     6901 cagcggacaa aactaaacag gaggttgctt catgaaaacc gtaattgcgg gcgccctggg
     6961 ggaatgcgtt cacgttgccg gcgtgatgaa ttttctgaac ctggcggaaa tcgcgggctg
     7021 gcgcacagtc tttctcgggc cggctgtgcc ggtggaagaa ttcctgcgcg ccgcaaggga
     7081 agaaggcgcg gacctggttg gcgcatccta ccgcctgacg cccgaaacag gcgagcgtct
     7141 gttgggtgaa tttgccgaag ccgcggatga actccgcgcg cagggcgtgc gctttgtgtt
     7201 cggcggcact ccgccggtgg cgcagcgcgc gcgcatgctg ggcttttttg agcgcgcgtt
     7261 cgacggcagc gaagctcccg aagaggtgct tgcctttctg cgcggggaga gcgccgaacc
     7321 gggcagcccc acgtattacc cccagaccac cctggaacgc attgcctgga aaacccccta
     7381 ccccctgctg cgccaccact ttggcctgcc taccatggca gctaccctgg agggcatcgc
     7441 ccaaatcgcg gaagcgcgcg ccttggatgt catttcgctc ggcaccgacc aggacgcgca
     7501 ggaaaatttt ttccacccgg agcgccaaaa cccgcgcgcc aaaggcgccg gcggcgtgcc
     7561 cgtgcgcagc gcggaagact accgcgcgct ctttcgggcc tcgcgccgcg ggaattaccc
     7621 gctcctgcgc acctattccg gcacggacga ttttatccgc ctggcagagc tctacatgga
     7681 aaccatcaac aacgcctggt gcgccgtccc gctcttctgg ttcaatcagc tcgacgggcg
     7741 cggtccggtc agcctggagg actctatccg cctgcaccag gagcttatcc gttggtacgg
     7801 ccagcgcggc gtgccagtgg aactgaacga accgcaccac tggggcatgc gcgacgcgcc
     7861 ggacgtgatc tttcttgtct ccgcctacct ttccgcctat aacgccaaag tttttggcgc
     7921 gcgcgattac atcgcccaga tgatgttcaa cagcccgccc ggcgcctccg acgcgatgga
     7981 cctggcaaaa atgctggcta ttctggaaat gagcgcagcg ctggaagacg aaaacttccg
     8041 catcttcaga cagacgcgca ccggcctgct cagctacccg ctggaaagta acgccgcccg
     8101 tgctcatctc tccgccagca tctacctgca gatggcgctc cagccgcaca ttgtgcacat
     8161 tgtcgggcat accgaagccg accacgccgc caccgccgct gatgttatcg aagccagcct
     8221 gatggcgcgc caggctgtcg ccaacgccct caaagggcag cccgatatgc tggcggaccc
     8281 gaaggtgcag gcgcgcaaag agcatctcat caccgaagcc cggctcacgc tcacggcaat
     8341 cagcaatttg gggcagctcg gcagcgaccc gctgagcgac cctgccgtac tgggtaaggc
     8401 cgtacgcctg ggcattctgg acgcgccgca cctgcgcggc agcaaaatcg cgcgcgggga
     8461 aatcagaacg cgcgtgattc agggcggctg cgaagccgtc cggtccgacg gcagccccct
     8521 tcccgaagcg gagcgccttt ccacctacct atgaaacgga gtaacgcatg aacgataacc
     8581 tgaaatacac agtcattgga gccggcaacg gcggcaaagc catggcggcg catatggcgt
     8641 tgatggggaa ggaggtgacg ctctttaacc gcactttcgc caacatcgaa gtcatcgccg
     8701 agcgcggcgg gattgacctg gaaaatccgg gcggcatcag cggtttcggc cggctggcaa
     8761 aggtcaccga taaaatcggg gaggcagttg cggacgcaga tgtaatcatg gtggtcgtgc
     8821 cgtcctccgg ccatcgggac attgccgccg cggccgcccc ctacctgcgg gacgggcaga
     8881 ttgtgctgct gcaccccggg cgcacctgcg gcgcgatcga attcgtcgag gttctgcgcc
     8941 aatccggatg caaaaccaag cctatcgtag ccgaggccga aactttcatc tacgccagcc
     9001 gttccgaagg gcccagccac gcaaaaatct tccgcatcaa ggaatccgtg ccgctggcgg
     9061 ccctgcccgc taccaaaaac cgcgcggtgc tggacgccat tcaccacgtc tatccccagt
     9121 acattgacgg cggcaacgtg ctcaagaccg ggctgaacaa catgggcgcg gtcttccacc
     9181 ccaccatcac cctgctgaac gccggctgga ttgaaaccac ccacggcgac tttgagtttt
     9241 atatcgacgg tgtttccccc tcggtcgcca aactgctgga gacggtggat cgcgaacgct
     9301 gcaccgtagc ttccgcgctg gggattcgcg cgcgcaacgg gttggaatgg ctggcgttgg
     9361 cctacgacgc ccgcggagag aacctgcacg aagccattca caaccagtcc ggctactacg
     9421 gcatcaaggc gccgcctacg ctgaatcacc gctacctgtt cgaggagatt ccgatgagcc
     9481 tggtgccgat tgcggcgctg gggcagcgct atggggtgtc tgtgcgcggg attgacgcga
     9541 ttatccgcct ggcgtgcatc attcacaaca cggattactg gcgcaaaggc cgcacgctgg
     9601 ataaactggg cattcaggac ctgagcattg aggaactgac gcactacgtg gaaaccggcg
     9661 agcgggaata agagataaag ttcgtatcca tcttgcaaaa gatgtaaact gaatggctta
     9721 gtttgtatac atttgggaaa agatgtatac aaagtaggcc agtttgtata catttgggaa
     9781 aagatgtata caaacaaagc gtgtttgtat acattaggta aaagatgtat actaaacttt
     9841 gttgtttgta tacattccga taagttgttt ggaataaaat gttttgtaca aattaatagt
     9901 agatggacat gagctagtga ataccgttca agttgggaca gtaagattat tgcctgctgg
     9961 ctaccaagcg ttcataccaa ctatttttcc acctaaagcg atgcttgatt ttccaaaaga
    10021 cattttaatg aaaaattctg atgctacttt aaaggtggga gagttaaaag gaattacacg
    10081 cttacttcct gatattgatt ttttcctttg gatgtactta cgcaaagacg cggcttcttc
    10141 tagtcaaata gaaggtactg ttgcaaccat gatagatgct atcgaagctg aagctaatat
    10201 caattcggat ttgcccgaag atgtagatga tattttgcat tatcttactg cattgcattt
    10261 tggtattcag cagatcgagg atggaaaact accgctctct ttacgattga ttaaagacct
    10321 gcattatcaa ttgctccacg aagcgcgtac cagtcatctc gccaaccccg gagaatttcg
    10381 gacttcacaa aactggattg gtggaacaac attaaataat gcacgctttg ttccacctcc
    10441 gccagcttat cttagtaatg ccctagggga tttagagata tttatgcatg cagaagatgc
    10501 cattcccatg ctcttaaagg caggattgtt acacgcacaa ttcgaaacaa tccatccttt
    10561 tcttgatggg aatggccgca ctggccgttt attgattacg ctctttttat ggaacaaaca
    10621 ttatcttgaa aaaccagtgc tttatttatc ctcttacttc catgcgcatc ggcaggaata
    10681 ttatgaccgc ctcgaaggat atcatcaggg aaaagtgttt gaatggctcg acttttacct
    10741 ggatggcata atgcaaacag caacagaggc gattgagaca gtcggtaaaa tcaccgctct
    10801 tcgagagaaa gatatgcgcc tcattcaggc cttgggtaaa cgttctgctg aactaattac
    10861 aaaacttctt ccaagacttt ttgctcagcc gatcattaat gtacaaacga tccaggattg
    10921 gactggttat acccgcgatg gggcttataa aatggttaca cgtctggttg gcttgggaat
    10981 tttagtccct cgctttaccg ataaaacata tggtcaaagt tttatgtata aaaactacct
    11041 ggatatcttt acagagagtt aatttcaaac tttatctgta ggtcgggtgc ggcggcgcag
    11101 gagcgcaacc cgacattgcg tgattcgtca ttgcgaagga gccagcgact gaagcaatct
    11161 cccaagaggc tgacggggag atcgcttcgt tcctcgcgat gacgaatgct ggaggtcggg
    11221 tggagttgta ggtcgggtgc agcgacgcag gagcacaacc cgacattgtg tgattcgtca
    11281 ttgcgaagga gccagcgact gaagcaatct
//
LOCUS       DAOQAD010000059        10917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_6914_length_10917_cov_4.912263, whole genome shotgun sequence.
ACCESSION   DAOQAD010000059 DAOQAD010000000
VERSION     DAOQAD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6914_length_10917_cov_4.912263"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PKL60_05890"
     CDS             <1..316
                     /locus_tag="PKL60_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNW95700.1"
                     /translation="RPPACPRGRPLLADALTSVAAILALLNATLLPLLKFLSCGLIIL
                     TLGLFSLVLNAAVFLLSSNIAQNVFHVGFEVKGFWPALLGSLIVSVITILFSREKRKE
                     SD"
     gene            506..790
                     /gene="rpsF"
                     /locus_tag="PKL60_05895"
     CDS             506..790
                     /gene="rpsF"
                     /locus_tag="PKL60_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNW95701.1"
                     /translation="MHTYELVYILQADLDEATLNGLTETVENLIKNANGEILKVDRWG
                     KRKLAYPIRKMTEGYYVLVSFKLDPAEVANLRRTLGYNEQILRSIVVRPE"
     gene            803..1078
                     /gene="rpsR"
                     /locus_tag="PKL60_05900"
     CDS             803..1078
                     /gene="rpsR"
                     /locus_tag="PKL60_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNW95702.1"
                     /translation="MSEYRSNNGGGNRHYVSRPKFCQFCADKNLAIDYKNVELMTRFI
                     NETGKIRPRRQTGTCARHQREVAKAIKNARHIALIPFEGDIYYDQNA"
     gene            1091..2461
                     /locus_tag="PKL60_05905"
     CDS             1091..2461
                     /locus_tag="PKL60_05905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNW95703.1"
                     /translation="MANNRIPEKQVVTKKHLAKQERERRETRTILIATLAIAAIVVGL
                     LGYVLVDNYIVKPSTVLATVGEQKITVRDFQPVVKFTRLNMLNQASNYYYYYQMLGSY
                     GSSFLTTAQNLITSLNSPSVIGEQVLNTMIEDILIEEEAAKRGITVSDAEVAEAMRAA
                     FDFYPEGTPTPTITPTLVNTPTLSLTQIAIIQPTDTPTPLPTSTATPEGWVPTNTATP
                     TLEPTATTEPVPAGSTATNVPTETKVPTITPTATAYTTKVYGGVMKDYYQNLSTYGIS
                     KDVVERSFRTQLLREKLLEDVTADLEPKQEQVWARHILVATEDEAKSVLEALNNGEDW
                     NTLAATYSTDTSNKDNGGDLGWFGRGTMVQAFEDAAFALEPGEISEPVKSDFGYHIIQ
                     CVGHAVNDLDASAFSTLKQTTFTDFLSALRTSRDDIVINERWVDFTPSTPAVTSQLYN
                     AIFGAQ"
     gene            complement(2889..6503)
                     /gene="smc"
                     /locus_tag="PKL60_05910"
     CDS             complement(2889..6503)
                     /gene="smc"
                     /locus_tag="PKL60_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNW95704.1"
                     /translation="MSQIKLASLEMHGYKTFARHTNLEFPARTTAIVGPNGSGKSNVA
                     DAIRWVLGEQSYSLLRAKKTEDMIYIGSDQRAQAGMASVSITFNNETNWLPIDYSEVV
                     LTRRAYRDGTNEYLLNNQRVRLKDFHELLAKTGLSDRTYTIIGQGLVDMALSIKPDER
                     SKLFEEAAGIGLYRSRREEALKRLESTQRNLERALDIVDEIKPRLRSLERQAARVGEY
                     KKVQENLQQKLRDWYGYHWFKAQQDIAQLKAELGSAEDASAAALALLHGNQAASGALK
                     ENISAQRELVNQLHQKLGNLHDRIQSANRDLAVNEEREQALGRAQAQISDDLAKLEET
                     ISSAEETIQTLRKEIETETQNLAQLQEKRAGVENELAQERNKKKELDDQQNKLQTDLL
                     SLEKQTLVLRSRKSEISSRLDSLLRSLQSNQESLQTIKQEEQAAFDSQSGIEKQILSL
                     EQEQQTIERQRKANQEKQLSIKRALEENSAQLNRYNLEKNKLTNQLQLLRQAQDSLAG
                     YSDGAKSLMKNSRVRQYGKTITDLATKLDVPEQYEQALTAALGEAVDLLVLPDDTLDK
                     DFLSGITDVVEGKVALIGLKGLPKRERQRAPSGDGILGTAADLVRSPGEMSALVQHLL
                     SQFLVVDSLERAFGLPDKLRESYDLVTLSGQVLLRSGVAVLGKQKSASKVSFVRTRNE
                     LEQALSVLEQTLTNALTKEQSLRQQLMDADQETAKNQNGQRALEKQIGELRRELNSAA
                     VNLDKAQNRRRWVEKQLEDNQQEVTRLQAEQNSLTGNEAGMRAESENLTEALESLKQS
                     MGEQSLDTLEHQFQYLQMECRVQEGALAHARDTLRAQEQRLLADKERMTLLNQRGSAN
                     AEELAVLRQKKSELNEVLQKLNEEVESIRANELAQIQAQLKGFESQAQVLSEEESALQ
                     KDLSFKERQVTHIQLELGRKLERLDSLRTHIEDDFGLIELEYRNEFSVSTPLPFPDLV
                     IETLAEIPVLPAGIEEDMRDLKNHIRRIGVVNFEAENEYAEVRERYANLTNQIADLQA
                     AILDIQRMITELNDVMQRAFLNTFKAVSVEFSKMFARLFNGGSARLILSDESSPIEGG
                     IEIEARLPGKREQGLVLLSGGERSLTAVALVFALLKISPTPFCVLDEVDAMLDESNVG
                     RFIELLKDLSSETQFLLITHNRNSVQVADVIYGVTMGKDGASQVISLRLDEVDESYVK
                     "
     gene            complement(6500..7204)
                     /gene="rnc"
                     /locus_tag="PKL60_05915"
     CDS             complement(6500..7204)
                     /gene="rnc"
                     /locus_tag="PKL60_05915"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNW95705.1"
                     /translation="MLDADASAKFYSALPGQFKDNFLLTRALTHRSYLNENRSVLEDN
                     ERLEFLGDSILGYVVAEWLYNHFPEKKEGVLTKLRSALVHTQQLADFARKINLGTVLL
                     LGHGEEQAGGRDRNAILCDAFEALIAAIYLSTDIATVKEFIYPFIEAEIQTILTNHSE
                     EDVKSLLQEWAQAQGFPSPVYLLTGEFGPDHEKVFSVKVLINDEEIAEGMGGSKQLAE
                     KSAAAAAITKIGIKEA"
     gene            complement(7298..8389)
                     /locus_tag="PKL60_05920"
     CDS             complement(7298..8389)
                     /locus_tag="PKL60_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNW95706.1"
                     /translation="MTTITCTSCALPYPEEGLPHLCPRCGGVFGIDSIDFPGEPGVEP
                     GMPGIWRYRASFSLPASAKAVYLGEGGTPLVEREIRGKRVAFKLENLNPSGSFKDRGT
                     AVLTSFMLSRSISEVVEDSSGNAGASLAAYSSAVGIKSRIFVPETASGPKVQQMVASG
                     ARVVRIPGPREAAHQAALADAQANKLPYASHALLPMGIGGFATIAYELAAQLGRMPGS
                     LIAPVGHGSLFLGLLLGARALCRQDPKVNLPVMVGVQPANCAPLAARWENRAFAGCQG
                     TSLAEGTQIEKPVRGEQILKLLRPGMDVMLAVAEEDLRLAYTELAEMGFYVEPTSAMV
                     WSALKKLIENLPEPIVLVFSGSGLKFRQS"
     gene            8470..9234
                     /locus_tag="PKL60_05925"
     CDS             8470..9234
                     /locus_tag="PKL60_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNW95707.1"
                     /translation="MRQTTSYEIETELYSGPLDLLLDLIQKAELDITKLALAQVTDQF
                     LAYIEAHRDLEPDYISEFLVIAAKLVQIKSEALLPRPPAREDDEEDPGEALAYQLQIY
                     KKIKGAAHWLGQRMDAKLRGYLHVPRAFDVHAQIDLTGVEVADLIAALEALLQQNAAS
                     EPESVISIPKLTLKRKLEEIISRLQVLPSTSFQQLLGAERTRLNMIVVFLAILELTKQ
                     DLVITEQSDLFADIRVTALAGALNEGGSEINIEDEN"
     gene            9300..9731
                     /locus_tag="PKL60_05930"
     CDS             9300..9731
                     /locus_tag="PKL60_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95708.1"
                     /translation="MSDTPNNEAPLQLEAGNTQTKKSNPWLIVGIVLVVLAVTAGIVV
                     LAGQSSEVTTKVRDIFIILLALEMFAIGTALIILIIQVAALTNLLQNEVKPILNSTTD
                     TVNTLKGTVKFLSNNVSEPVIKLNQSLASLNRLLELLKLRK"
     gene            9760..10086
                     /locus_tag="PKL60_05935"
     CDS             9760..10086
                     /locus_tag="PKL60_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNW95709.1"
                     /translation="MSESNNFGSFVMGFVVGALSGAIASLLMAPQTGDETRQLIKDKT
                     IELKEKGGSTFEETKRKAEEAYKEAVTKAQELGTTAAEKVSETVKKARKTVEVPPIET
                     PEAPAE"
     gene            10225..10298
                     /locus_tag="PKL60_05940"
     tRNA            10225..10298
                     /locus_tag="PKL60_05940"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10259..10261,aa:Asp,seq:gtc)
     gene            10498..>10917
                     /locus_tag="PKL60_05945"
     CDS             10498..>10917
                     /locus_tag="PKL60_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95710.1"
                     /translation="MFAPAKPDFHPLANPLAVLTSGNVRFTILKSRLIRIEYAPNGQF
                     EDRPSQVFWHREQALPEFSARQTDEWLIIETEHLHLRYKAGEELNYRYLQITLKETGI
                     TWHYGDPDHTNLGGTARTLDRANGRIPLGQGLVSRSGW"
BASE COUNT         2485 a         3125 c         2723 g         2584 t
ORIGIN      
        1 gcggccaccg gcctgcccga ggggacgccc gttgctggcg gatgcgctga catctgtggc
       61 agcgattctg gcgctgctga acgccacgct gctgccgctg ctgaagttcc tctcctgcgg
      121 cctgatcatc ctgacgcttg gcttgttcag cctggttttg aatgccgctg tctttttgct
      181 gtcatccaac atcgcccaaa atgtatttca cgtgggcttt gaagtgaaag gcttctggcc
      241 tgccctgctc ggctcgctga ttgtcagcgt gattaccatc ctgttcagcc gcgaaaaacg
      301 caaagagtca gattaacccc tccctttcga aaggtaccgg cacccgccgg cacctttttt
      361 gatttcagcc acgcgtttgc ttgcgaaaat tccagacttg tgataaactg ccttccgtta
      421 aattcaggaa aaacctgaaa ccttccactc ctgcccgcca acggcgcaca gcaggagcca
      481 acccgaggaa aggagcctca tacttatgca tacttacgag ttagtttata ttctccaggc
      541 agacctggac gaagccacat taaatgggct taccgaaaca gttgaaaacc tcatcaagaa
      601 cgcgaacggc gaaatcctta aagtggaccg ctggggcaag cgcaaactgg cctatcccat
      661 ccgcaagatg accgaagggt attacgtgct ggtatccttc aagctcgatc cggccgaagt
      721 cgcgaatctt cgccgcactt taggttacaa cgaacagatt ttgcggtcta ttgttgtcag
      781 accagaatag aaaggaatag aaatgtccga ataccgatcc aacaacggcg gcggcaaccg
      841 ccattatgtt tccagaccaa aattctgcca gttttgtgca gataagaacc tggcaattga
      901 ctacaagaac gtcgagctta tgacccgctt tatcaatgag accggcaaga tccgtccccg
      961 ccggcagact ggcacctgcg ccaggcacca gcgcgaagtt gcgaaagcaa ttaagaacgc
     1021 ccgccacatc gccttaattc cttttgaagg tgacatctat tacgatcaga acgcttagag
     1081 gtgattcttt atggcgaata accgcattcc agaaaagcag gttgtgacaa aaaaacacct
     1141 tgcaaaacag gaaagggaac gtcgcgagac cagaacaatt ctgatcgcta cgctcgctat
     1201 cgccgccatc gtggtgggtt tgctcggtta cgtattggtc gacaattaca tcgtaaaacc
     1261 atccacagtg cttgccactg tcggagagca aaaaataact gtccgtgatt ttcagccggt
     1321 ggtgaagttc acccggttga acatgttgaa ccaggccagt aattactatt actactacca
     1381 gatgcttggc agctacggct ccagctttct gaccaccgcg cagaacctca taacgtctct
     1441 gaacagtccg tctgtaatcg gtgagcaagt gctaaacacc atgattgaag acatcctgat
     1501 tgaggaagaa gccgccaaac gcggcatcac cgtcagcgat gcggaagttg cggaagcaat
     1561 gcgagctgcc ttcgatttct atcccgaagg cacacccacc ccgaccatca cacccacttt
     1621 agtgaatacc cccacgctgt cgctgacaca aatcgccatc attcagccga ctgatacccc
     1681 taccccgctg ccgacctcaa ccgcgacacc tgagggttgg gtgccgacca acacggctac
     1741 gccgactctg gaaccaactg ccacaaccga gcccgttcca gccggctcga ccgcaaccaa
     1801 cgttccgacg gaaaccaaag tgccgacgat tacgccgact gcaaccgcgt acacgaccaa
     1861 agtgtacggt ggtgtgatga aagactatta tcagaacctg agcacgtacg gcatctcgaa
     1921 ggatgttgtc gaacgttcct tccgtacgca attgctgcgc gagaagctgc tggaagatgt
     1981 cacagcggac ctggaaccca aacaggagca ggtgtgggcg cgccatattc tggtagccac
     2041 cgaggacgaa gcgaaatcag tcctggaagc ccttaataac ggcgaagact ggaataccct
     2101 ggcggccacc tattccaccg acacctctaa caaagacaac ggcggtgatt tgggctggtt
     2161 cggacgcggc acaatggtgc aggcttttga ggatgccgcc tttgctttgg aacccgggga
     2221 aatcagcgag cctgtaaaat ctgattttgg ttaccatatc attcagtgtg tcggacacgc
     2281 tgtcaacgac ctggatgcct ctgctttctc gacattaaag cagaccacct ttactgactt
     2341 cttatctgct ctgcgcacat cccgcgatga catcgtgatt aacgagcgct gggtggactt
     2401 cacccccagc accccggcag taacgtccca gctttacaac gcaatttttg gcgctcagta
     2461 gacccaaacc aaccatcaag gggctgtcct aaaagtaaga cagccccgga tgatttccca
     2521 ctccgtgccc gtttttatgg gcgtctgcgg atttgcgctg gcaaatcgac gcctaaacca
     2581 ggcactttct cccactatgg acagcctctt ttgttttcct tagttggata agtctcctct
     2641 cggatcgcat tatcgctcaa aaaccctatg aattacgcgc agcgcggaga gagtcgattg
     2701 ctaaagcccg acggctaaaa ccaatccccg ccgcaaaaaa agccgtaatc gactttctca
     2761 ggggtctatt accgtctaag gccgcttggt ttgcggcgaa ccttgtcttc aacggcaggg
     2821 ggaatgcttc aacagaacct gcccaactca acgattaccg aaaaccgtac aacaaaaata
     2881 ctccccgact atttgacgta actctcgtcc acctcgtcca ggcgcaggct gatcacctgg
     2941 ctagcgccgt ccttgcccat tgtcacgccg taaatcacat ccgcgacctg gacggagtta
     3001 cggttgtgcg tgatcagcaa aaactgggtc tcgctggaaa gatcttttag cagctcgata
     3061 aacctgccca cgttggattc atccagcatg gcgtccactt catcgagcac acagaaaggg
     3121 gtgggagaga ttttcaacag ggcaaagacc agcgccaccg cggtcaggct gcgttccccg
     3181 cccgataaca gcacaagacc ctgctcgcgt ttccccggca ggcgcgcttc gatttcaatt
     3241 ccgccttcga taggcgagct ttcgtccgat aggatcaggc gcgcggagcc gccgttaaac
     3301 aatcgtgcga acatcttgct gaattcgacg cttacagcct taaaggtatt caagaacgcg
     3361 cgctgcatca cgtcgttcag ctctgtaatc atgcgctgga tgtccagtat cgcagcttgc
     3421 aaatccgcga tttggttcgt caggttggca tatctttctc tgacttccgc atattcattc
     3481 tcagcctcaa aatttactac tccgatgcgg cggatgtggt tcttgaggtc gcgcatatct
     3541 tcctcaatcc ccgcgggtaa aaccggtatt tctgccagcg tttcaatcac caaatccgga
     3601 aaaggcagcg gtgttgaaac cgaaaactca ttccggtatt ccaattcaat cagcccgaaa
     3661 tcatcctcaa tgtgtgtacg cagcgaatcc agcctctcca gcttgcgccc gagctccagc
     3721 tgaatatgcg tgacctggcg ttctttgaaa gataagtcct tttgcagtgc gctttcttcc
     3781 tcactcagaa cctgggcctg gctttcaaag cctttgagtt gggcttggat ttgtgccagt
     3841 tcattggcac ggatagattc cacttcctcg ttcagctttt gcagaacttc gttcagctca
     3901 gacttcttct gcctcaaaac agcaagttct tccgcgttcg cgctgccgcg ttgattaagc
     3961 agtgtcatgc gttctttatc cgcaagcagg cgctgctcct gggctctcaa agtatcccgg
     4021 gcatgcgcca acgccccttc ctggacccgg cattccatct ggagatattg gaattggtgc
     4081 tccaatgtat ccaggctttg ctcgcccatg ctttgtttca aggactcgag cgcttccgtc
     4141 aaattctctg attccgcgcg catgccagcc tcgttcccgg tcaggctgtt ttgctcggct
     4201 tgcagacggg tgacttcctg ttggttgtcc tccaactgtt tttccaccca tctgcgccgg
     4261 ttctgggcct tatccaggtt gaccgcggcg ctgttcagct cgcggcgcag ctcgcctatt
     4321 tgcttttcca gtgcgcgctg cccgttctga ttttttgcag tctcctggtc agcgtccatc
     4381 agctgctgtc gcagactctg ctcttttgtg agcgcattgg tgagcgtctg ttccaggacg
     4441 gacagcgctt gttccagttc attacgcgtg cgtacaaaac ttaccttgct ggcgctcttt
     4501 tgtttcccga gcacagcaac gccgctgcgt agcagaacct ggccacttaa cgtcaccagg
     4561 tcgtagcttt cccgcagctt atcgggcaaa ccaaaagccc gttccaggct gtccacaacc
     4621 agaaattgac tcaggaggtg ctgcaccaga gcggacattt cccccgggct gcgcaccagg
     4681 tccgccgccg tccccagaat tccatctccg gaaggtgcgc gctgcctttc ccttttcggc
     4741 agacccttaa gaccgataag cgctactttc ccctcaacga cgtccgtgat gccgcttaag
     4801 aagtctttgt ccagggtgtc atccggcagt acaagcaagt ccacagcttc gcccagcgcg
     4861 gcggtaagcg cttgctcgta ctgctcggga acgtccagtt tcgttgccag gtcggtaatc
     4921 gtttttccgt actgcctgac ccgcgaattt ttcatcagcg atttcgcgcc gtccgaatag
     4981 cctgccaggc tgtcctgcgc ctgacgcaag agctggagct ggttcgtcag tttatttttt
     5041 tccaggttat agcggttcag ttgggcggag ttttcctcca atgcccgttt aatggacagc
     5101 tgcttttcct gattcgcttt gcgttgacgc tcaatcgtct gctgttcctg ctccagcgac
     5161 aaaatttgct tctcgatacc gctttgagag tcaaaggcag cctgttcttc ctgcttaatc
     5221 gtctggaggc tttcctgatt gctttgcagg ctgcgcagta aggagtcaag gcggctggag
     5281 atttcgcttt tccggctgcg cagcaccagc gtctgttttt ccaggctcaa caaatcggtt
     5341 tgcagcttgt tttgttggtc gtccagttct ttcttcttgt tccgctcttg cgccagctca
     5401 ttctccaccc ctgcgcgctt ttcctgcagc tgtgccaggt tttgcgtttc ggtttcaatt
     5461 tcttttctga gcgtctggat agtttcttca gcggaggaga tggtttcttc aagctttgcc
     5521 aggtcatcac tgatttgtgc ctgcgcccgc ccaagcgcct gctcgcgttc ctcattcact
     5581 gccaggtcgc ggtttgcaga ttgaattcgg tcatgcaggt ttccaagctt ctggtgcagt
     5641 tggtttacaa gctccctctg tgcggaaata ttttctttca gcgcgccgga agcagcctga
     5701 ttcccgtgca aaagggcgag tgcggcagca gaagcatcct cagcgctacc cagttcagcc
     5761 ttgagctggg cgatgtcctg ctgagctttg aaccaatggt atccgtacca atcacgcagc
     5821 ttttgctgca gattttcctg taccttttta tattccccca cgcgcgccgc ctgtctttcc
     5881 agactgcgca gacgaggttt gatttcatcg acaatatcga gcgcgcgttc aaggttacgc
     5941 tgggtgcttt ccaggcgctt cagggcttct tcccttcggg aacggtataa gccaatgccg
     6001 gctgcttcct cgaacaactt gctgcgttcg tcgggcttga tcgagagcgc catgtccacc
     6061 agcccctgac cgataatggt gtaggttcgg tcgctcaggc cggttttggc caatagttcg
     6121 tgaaaatcct ttagccgcac gcgctgatta ttcagcaggt attcgttcgt cccgtcgcgg
     6181 taagccctgc gggtgaggac aacctcgctg taatcaatgg gcagccagtt cgtctcgtta
     6241 ttaaacgtaa tggaaacaga agccattcca gcctgcgcgc gttggtccga cccgatataa
     6301 atcatatcct cagttttctt ggcgcgcagg agcgagtaag actgctcgcc cagcacccaa
     6361 cggattgcat ccgccacgtt ggatttaccg gagccattcg gaccgacaat cgcagttgtg
     6421 cgcgcaggga attccaggtt agtatgccgc gcgaaggtct tgtacccgtg catttcaagc
     6481 gaggcaagtt tgatttggct catgcttctt tgattccgat tttcgtgatt gctgcagccg
     6541 cggctgattt ttccgcgagc tgtttacttc cccccatgcc ctcagcgatt tcctcgtcat
     6601 ttatcagcac ttttactgaa aagaccttct catggtccgg tccaaactca ccggtgagca
     6661 ggtataccgg ggaaggaaat ccttgcgcct gcgcccattc ctgcagcagg cttttaacgt
     6721 cctcttcgga gtgattcgtc aggattgtct ggatttcagc ttcaatgaag ggatagatga
     6781 attctttgac ggtcgcgata tccgtgctaa ggtaaatggc ggctatcagc gcttcaaacg
     6841 cgtcgcataa gatcgcgttc cggtcgcgtc cgccggcctg ctcttcgccg tgcccaagca
     6901 gcagcaccgt gcccaggtta attttccggg caaaatcggc taactgctgc gtatgcacca
     6961 gggcggagcg cagtttggtg agcacaccct cttttttctc agggaaatgg ttatacaacc
     7021 actccgccac gacgtatcca aggatggaat cccccagaaa ttccagccgc tcattatctt
     7081 ccaggacaga gcggttttca ttcaggtatg agcggtgagt gagcgcgcgg gttaataaaa
     7141 agttgtcttt aaactgccct ggcaaggcgc tgtaaaactt tgcagaagca tcggcatcaa
     7201 gcatgcgttt cctgttctga actgcctgat cgtctgccat caatcgttca actgcaaaga
     7261 tgcttttgat ggcaaacgat ccaacaggtc gtgagtatta tgattgtctg aattttaacc
     7321 cagaaccgga aaaaaccaaa acgattggtt cgggaagatt ttcgatcaac ttcttcaacg
     7381 cggaccaaac catcgcggac gtaggttcca cgtaaaaacc catttccgcc agctctgtgt
     7441 aagcgagacg caagtcttcc tcagcgaccg ccaacatgac gtccattccc gggcgcagca
     7501 acttcaaaat ctgttctccg cgcacgggtt tctcgatttg agtcccttca gccagagaag
     7561 tcccctgaca gcctgcaaat gctctatttt cccagcgggc tgccaatggc gcgcagttag
     7621 ccggctgtac gcctaccata accggtaagt tcacctttgg gtcttgccgg cagagcgccc
     7681 gggcgcccag cagcaaaccc aggaagagac tgccgtgccc caccggcgcg ataagcgacc
     7741 ccggcatccg cccgagctga gctgccagtt cataagcaat ggtggcaaat ccaccaatcc
     7801 ccatcgggag gagtgcgtgg ctggcgtagg ggagtttatt tgcttgcgca tcggcaagcg
     7861 cggcttgatg ggcagcttcc cggggaccgg gaatccgtac tacccgcgcc ccgctggcaa
     7921 ccatctgctg aactttgggt ccggatgcgg tttcgggtac gaagatacgg cttttgattc
     7981 ccacagcgga ggaataagct gccagagatg ctccggcatt tccggaagaa tcttccacga
     8041 cttcgctgat gcttctggaa agcatgaaac tggtaagcac tgccgtcccg cggtctttaa
     8101 acgagccgct tgggttcagg ttttcgagtt tgaaggcgac ccgcttccca cggatttccc
     8161 gctctacaag gggtgtgccg ccttcaccca gataaaccgc tttcgcactc gctggcaggc
     8221 taaaactggc tctgtagcgc cagatgccag gcattcccgg ctccacaccg ggctcaccgg
     8281 ggaaatcaat gctgtcaatt ccaaaaacgc cgccgcagcg cgggcacaga tgcggcagcc
     8341 cctcctcagg gtaaggcaaa gcgcaggaag tacaggtgat ggtggtcaca gacttagtat
     8401 cgcttatttt tgtgaattca gcaatagccc tccggcttca gctataatta ctgcatgacc
     8461 aagagttggt tgagacaaac cacaagctat gaaattgaaa ccgagctgta ttccggaccc
     8521 ctcgatctgc tgctggacct gattcagaag gcggaactgg acatcaccaa gctggcgctg
     8581 gcacaggtta ccgaccagtt cctggcctac attgaggcac accgcgacct ggaaccagat
     8641 tatatttcgg aattccttgt catcgccgcg aaactggtgc agataaaatc cgaagcgctc
     8701 ctgccgaggc cgcctgcccg ggaagacgac gaagaagacc ctggtgaagc cttagcttac
     8761 cagctgcaaa tctacaagaa gatcaaaggt gcagctcatt ggcttggaca aaggatggac
     8821 gccaaactgc gggggtacct gcacgtcccg cgcgcttttg acgtacacgc gcaaattgac
     8881 ctcacaggcg ttgaggtagc ggatctgatt gccgcgctcg aggcgctcct gcaacaaaat
     8941 gccgcttccg aaccggaaag tgtcatcagc atccccaaat taacgctcaa gcggaaactc
     9001 gaggagataa tcagccgtct gcaagtgctg cccagcacgt ctttccagca gctcctgggc
     9061 gcggaacgca cccgcctgaa tatgattgtg gttttcctgg ccatcctgga gcttaccaag
     9121 caggacctgg tcattactga gcagtccgac ctcttcgcgg acatccgcgt aaccgcgctg
     9181 gcaggcgcgc tgaatgaggg cggcagcgag ataaatatcg aggacgaaaa ctgaacaaag
     9241 cttttaccct ttcacagaat gcttcgggca tggtttataa tcaatgagga aggataatta
     9301 tgagcgacac accgaacaat gaggcacctc tgcagctgga agctggcaac acacagacga
     9361 aaaagagcaa cccatggcta attgttggga ttgtccttgt agttctggct gtcaccgcag
     9421 gcatcgtcgt gcttgccggg cagagcagcg aggtcacaac caaagtgcgc gatattttca
     9481 ttatcctgct cgcgctggaa atgtttgcaa ttggcactgc cctgattatc ctcatcattc
     9541 aggtggctgc gcttaccaat ctgctgcaaa atgaagtcaa gccgattctc aattcgacca
     9601 ccgacacggt gaacactttg aaaggcacag tcaagttctt aagcaacaac gtgtccgaac
     9661 cggtcatcaa actaaatcaa agtctggcaa gtttgaacag actgcttgaa ttacttaaat
     9721 tacgcaagta accattacca aacgaaacag gagaatacca tgtcagagtc taacaatttt
     9781 ggatcctttg tgatggggtt tgttgtcggc gcgctctcgg gcgcaattgc ctcgctatta
     9841 atggcgcccc agaccgggga cgaaaccagg cagctaatta aagacaaaac gatcgagttg
     9901 aaagaaaaag gcggcagcac cttcgaagaa accaagagga aagccgaaga agcttataaa
     9961 gaggctgtca ccaaagcaca ggaattgggc accacagcgg cagaaaaagt atccgaaacg
    10021 gtaaagaaag ccaggaaaac cgttgaggtc ccacccattg agacccccga agcgccagca
    10081 gagtaaaagg cagcgcttct cagcagcgaa aaaagcggct ggttttgtgc atcgcgatgt
    10141 ttttttgcgc gggcaataag gcatttttcc aggccattct gcttgactta tgccatttgc
    10201 cgtgataaac tagctttcgc ttttgggggc gtggtgtagt ggtcgaacat gtggccctgt
    10261 caaggccaag atcgcgggtt caaatcccgt cgctcccgca ccgcagaggt catccatccg
    10321 gatgacctct ttttttccga taaatcaaaa ccggctgcct ttcacatact tcagcgatgg
    10381 ggaacgaacg cttgtcctcc tcattcccaa gcgagaatcc ctaatccact ctgcagtccc
    10441 gtctgagcta aattccggta caatagactt tacgttccaa atagtctgtg aggtctgatg
    10501 ttcgcccctg caaaacccga ttttcatccg cttgccaacc ctcttgccgt gcttacatcc
    10561 ggaaacgtgc gctttaccat cttgaaaagc cgcctgattc gcattgaata cgcgccgaat
    10621 ggtcaattcg aagaccgccc cagtcaggtt ttctggcacc gcgagcaagc cctcccggaa
    10681 ttttccgccc ggcagactga tgaatggctg ataatcgaaa cagaacattt gcacttgcgc
    10741 tacaaagcgg gcgaggaatt gaactaccgc tacctgcaaa tcaccctgaa agaaaccgga
    10801 attacctggc attatgggga ccccgaccat accaatctgg gcggcacagc ccgcacgctc
    10861 gaccgtgcca acggcaggat acccctgggt caaggcctgg tgtcgcgctc cggctgg
//
LOCUS       DAOQAD010000060        10767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_7038_length_10767_cov_6.504574, whole genome shotgun sequence.
ACCESSION   DAOQAD010000060 DAOQAD010000000
VERSION     DAOQAD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7038_length_10767_cov_6.504574"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..3035)
                     /locus_tag="PKL60_05950"
     CDS             complement(183..3035)
                     /locus_tag="PKL60_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNW95711.1"
                     /translation="MSENLTNAPGGEPKSVSVSWHALNALEVLSALNVPADAGLTTEE
                     AEKRLAQYGPNELQEKKRTTFLQMLWAQINSFVIWLLIGAALISALLGDYVEAGAILL
                     IVILNAIMGIIQESRAEQSLAALKKLAAPEAQVLRDGHRVSLPAPKLVPGDIVFIEAG
                     NYIPADVRLLEAVNLSVEEASLTGESVPANKNAEGALEADIPIGDRKNSAFMGTLVTY
                     GRGKAVVTSTGMNTQLGLIASMLQTVEEEQTPLMKRLDQLGKTLGIGAIVVCALVFAI
                     GVARLLLGPEHLSFSNPNFVNELVNLFMIAVSLAIAAVPEGLPAVVTISLAGGMREMV
                     SRHALIRKLSSVETLGSVTVICSDKTGTLTQNQMTVTRLWTDGKTFSITGSGYDPVGD
                     FLLDDAKVDLQDYPAARTALWIGTLNNDAQLEVTGESEGKITYRMVGDPTEGSILVAA
                     HKAGASARALNEAYPRLQEIPFDSDRKRMVTINGLRNPSVEDISPFAQSDRNKWHVIA
                     VKGAPDVVLKLCAYKLRLDNSYEPLTDASRQEILAANDAMTRSALRVLGVAYRLVPEM
                     PTDTSPEALEQELVFIGLIGMIDPARSEVKPALEEARTAGIRTVMITGDYPNTAKAIA
                     EEIGLLQPGHRVMTGAEIDDISAEDMREVARVTDVYARVSPEHKMKIVDALRRNNEVV
                     AMTGDGVNDAPAIKLADIGVAMGITGTDVAKETADMVLTDDNYASIVSAVEQGRIIYS
                     NIRKFVYYLVSCNMAEIMTIFLGTLFGWPPILTAIQLLWLNLVTDGAPALALGSEKGD
                     PDIMLFPPRPTNEPIINKFMLRGIIVQTIAITASTLGAFALGNSIDIGLGETMAFVTL
                     SVSELLRAYTARSEYYPLTKIGIFSNKGMNIAVLASLALIAAVVYVPFLQPIFNTSPI
                     DFGHWLMILPLILLPSIAAEVMKAVQFKRITKANTI"
     gene            3206..4225
                     /gene="holB"
                     /locus_tag="PKL60_05955"
     CDS             3206..4225
                     /gene="holB"
                     /locus_tag="PKL60_05955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNW95712.1"
                     /translation="MGWNICGHETAVAFLTEHTRPEKLRHAYLITGPQGVGRRTLALA
                     FIKALNCQNPPAPGEFCDSCQACRQINARSFADLAVLAPDEGHQDLRIDQIRAVQQSL
                     ALAPYQAKYRVVLIQDFQRATAAASNALLKSLEEPPARAVLVLTADAEENLLPTIVSR
                     CQIVRLRPLPMEKAGAWLEEKLQVPTERAALLAHLSSGRVGAALRFEQDPELLRGYED
                     ALDQLEELLCSSRRQRLQYVEKLQKGKGAQRELAGQLVSAWLSFWRDALIVRSGADMP
                     LVNQERQGFLREAAGQMEFQRIEAVLEQHEKALAQLEAYVNPRLTLECLLLTLPILKA
                     AWGRQ"
     gene            4222..5403
                     /gene="chrA"
                     /locus_tag="PKL60_05960"
     CDS             4222..5403
                     /gene="chrA"
                     /locus_tag="PKL60_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNW95713.1"
                     /translation="MRFAKPAIQPEQKAKLKDLIRVFLQIGATSFGSPTAHIAMMESE
                     IVRKRKWLTGEHFLELLAATNLVPGPNATELALHIGYLQAGFPGLLAAGASFILPSFI
                     ATLTLAWAYVRYGALPQADAIFYALNPLVLAIVINTLWNLGKGCLKSWQTWLIFGLAL
                     TGSLLGVSELIVLFGAGLLGYVLLDLIKNPPKAPAALLAFFPFPLLFLRFFAQASAWL
                     QSRTVQLFLYFLRTGAVLFGSALVLFPLIREDVVNRFGWMSQQQLIDAIAVGQMTPGP
                     VTSAVTFIGYNVGGVPGAFASTIGVFLPSFIIVAAMGPLMKKLADSKAAKAVLGGVNA
                     GVIALIFTIALTMAKNAVVDVWTGLLLAAGLAGLFLLKLTPYWLVLAGFLVGLAKVLF
                     F"
     gene            5432..7612
                     /locus_tag="PKL60_05965"
     CDS             5432..7612
                     /locus_tag="PKL60_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNW95714.1"
                     /translation="MNDLLELLNPQQQSAVAAPAGPTLVLAGPGSGKTRVLTYRIAYL
                     IGVLHVPAYHILAVTFTNKAAKEMAARVEKILGAAAAGIWLGTFHSVCGRILRREADL
                     LPLSKDFVIFDADDQVTLMKEVIREAGVDEKLFRAESVLDAISRAKNDLISPEDYPIQ
                     NFRDEKVRQFYLRYQQLLVRNNAMDFDDMLVFTYQLLSTYPQTRSTWAHKFEHILVDE
                     FQDTNLSQYAILRSLAQEHGNLYVVGDEDQSIYRWRGADYRNIQRFQSDFKNAQTILL
                     EQNYRSTQRILDGAMAVIDRNANRTRKKLFTEQRQGEKIKLFIAADDKEEAEYVVQAV
                     KDGIRTQKQNPSSFAVMYRTNAQSRLLEESFRREGLSYRLVGAQRFYGRREVKDVISF
                     LRLIHNPRDEISLRRVINLPPRGIGGKSLEKLEEYARAADLTLGEAVARLAEPAGRAA
                     LEQLGKEGLRLEQFGKKLLFWRQYAAGNPPASLFDRVLEDTGYQEYIHDRSEEGQDRW
                     ENVMELRQVILEYDGLPGFLEAMALVADQDTLPETLDAPTLLTLHAAKGLEFENVFIV
                     GLDEMILPHSRSRDDPEALAEERRLLYVGITRAKKTLTLTRAQRRRSPYGSYEETQAS
                     RFLDDLPEDLLQGSAKGLPSRLNIQYQSAYSRWETPAYRAAPRQDTPQNTKYKAGMLV
                     RHKTYGRGVVKASRLEHGDETVEVVFEGFGLKALVASLAKLEIL"
     gene            7769..9259
                     /locus_tag="PKL60_05970"
     CDS             7769..9259
                     /locus_tag="PKL60_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95715.1"
                     /translation="MKTRRALLLAAVGVLLLGLACRYTAPTPAAWAPSSSAELDARTR
                     SARLTASALEETRSSPNSPTATSTPLPRPTTAEDGPWLIFPDAAGEHLFALDRDSGTL
                     TRIPLPMLTDPLDIIGGRAPDGARVLLRAGTPDNLGELGLYELVSPWEDPRMLTSLLS
                     YELQEDILQNNGIQAQMALQAVQQPHPVSWSADGKAAVFPAALDGPSADLYLYHPGDS
                     ALVRLNQRFQQDFSPLWSPQQDWIVFQEVNSYATTNAWKLSLVGALSMPNGAVTHYLH
                     VPRDTGYWERYVGWMSALYLLTCTPTDNGAVDLRLSNVSAGKPRVIFDGAFAEAAVNP
                     QNGELAVVVRAENNNPRYPAGIYYSSNNGTTFTALLAGEYRNLEYSSENGLFTASNAV
                     GVVQFDSSGSVFILAGEERLGISPDGHWLIGWGGNGARLYSPQGFELQTLTEQSVSAL
                     AWQEDAGGFFLLEEDGLYYLQFPLLQARLVTQDVWRGDERAFVWLR"
     gene            9332..10021
                     /locus_tag="PKL60_05975"
     CDS             9332..10021
                     /locus_tag="PKL60_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNW95716.1"
                     /translation="MSTIFTWYGHATIGLDCDGIKLVVDPFFSGNPAAAVKAQQVRAD
                     YLLISHGHSDHVGDALDIARRTGATVVANAEISSWFSGKGVKTHAQHIGGGFRYPFGY
                     LKLTQALHGSALPDGSNGGNPAGFLLSTNAGEKIYLAGDTGLFGDMRLIGEEGLDLAA
                     LPIGDNYTMGPDDALRAVKLLQPKVVIPIHYNTFPLIQQDVQAWKKRVEAETGARVEA
                     LKPGGTYTLGA"
     gene            10022..10642
                     /locus_tag="PKL60_05980"
     CDS             10022..10642
                     /locus_tag="PKL60_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNW95717.1"
                     /translation="MTQKSEHPNPKPGRIPPGQLLTKRFPVLHYGPVPSFDPGTWTFR
                     VWGEVEKPLALTWEEFCALPRTALTMDLHCVTTWSKLDTHWEGVSLATLRELGLVVPT
                     AGARYVIQHAEFGFTANLPIEVAEADNFLLATHYEGQPLTPEHGCPLRGVTGAIPGRD
                     DLKDVYLWKGAKWLRGLEFSAHDRPGFWERAGYHNDADIWLEQRTA"
BASE COUNT         2154 a         3143 c         3287 g         2183 t
ORIGIN      
        1 cctacatgct gcacccaacc aacacctgca cccagcctag cacttgttgg gtttcactcc
       61 tgcgtcgctg cacccaacct acaaatctga gtcgcaagcg gctgaatgct aacgctgtac
      121 atcgctgaat gcaggcaaaa aaagaacctc cccgtaggga ggctcctctt ctgacacaac
      181 aattagatcg tatttgcctt ggtaatccgc ttgaactgga cggctttcat cacttccgcc
      241 gcaatggagg gcaggagaat cagcggaagg atcatcagcc agtgaccaaa gtcgatcggg
      301 ctggtattga aaattggctg caggaagggt acatagacta ccgctgcgat taacgcgagt
      361 gaagccagca ccgcgatgtt catgcctttg ttgctgaaga tgccaatctt ggtcagcggg
      421 taatactcgg aacgcgctgt gtacgcgcgc agcagttcgc tgacagaaag cgtcacgaaa
      481 gccatggtct ctcccagtcc gatgtcgatg ctgttcccga gggcgaacgc gcccaacgtg
      541 gatgcagtaa ttgcgatggt ttggacgata atcccgcgca gcatgaactt gttgataatc
      601 ggctcgttgg tcgggcgcgg ggggaagagc atgatatccg ggtcgccctt ctccgaaccc
      661 aaagccagcg ccggcgcgcc gtccgtcacc aggttcagcc acaacagttg gatggcggtc
      721 agaatcggcg gccagccgaa cagggtgccc aggaagatgg tcataatttc ggccatatta
      781 caggacacga ggtaatagac aaacttgcgg atgttgctgt atatgatgcg gccttgctcc
      841 accgcggaaa cgatagacgc gtaattgtca tcggtcagca ccatatccgc ggtttccttg
      901 gccacatccg tgccggtgat gcccatggcg acgccaatat ccgccaattt gatcgccggc
      961 gcgtcgttca ctccgtcgcc agtcatcgcg accacttcgt tgttgcggcg cagcgcgtcc
     1021 acgattttca tcttgtgctc ggggctcacg cgcgcgtaca catcggtcac ccgggctact
     1081 tcgcgcatgt cttccgcgct gatgtcgtct atttcggcgc cagtcatcac cctatgccca
     1141 ggctgcagca ggccaatttc ttcggcaatc gccttggcgg tgttggggta atccccggta
     1201 atcatcacgg tgcggatacc agcggtgcgc gcttcctcga gagctggttt gacctcggag
     1261 cgggccgggt ctatcatgcc aatcagacca atgaagacca gttcctgttc aagcgcttcc
     1321 gggctggtat cggtgggcat ttcgggcacg agccggtaag ccacgcccaa aacgcgcagg
     1381 gcgctgcggg tcatcgcatc gttggctgcc aggatttcct gccgggaagc gtcggtcagc
     1441 ggctcatagc tgttatccag gcgcaattta tacgcgcaca acttcagcac cacgtccgga
     1501 gcgcctttga cggcaatgac atgccatttg ttccggtcgg actgtgcgaa gggtgaaata
     1561 tcttcgacgc ttggattgcg cagcccgttg atcgtgacca tgcgtttccg gtcggaatca
     1621 aagggaatct cctgcaggcg cgggtaagcc tcgttgagcg cgcgggcgct ggctccggcc
     1681 ttgtgggcag ccaccagaat ggaaccctca gtggggtcgc caaccatacg gtaggtgatt
     1741 ttcccttcgc tctcgccggt aacttccagc tgggcgtcgt tattcagcgt gccaatccac
     1801 agcgcggtgc gggcggctgg gtaatcctgc aggtccacct tcgcgtcgtc cagcagaaaa
     1861 tcgccaaccg ggtcgtaacc gctgccggta atcgagaagg tttttccgtc tgtccataga
     1921 cgggtgaccg tcatttggtt ctgggtgagc gtgccggttt tatccgagca gatgaccgtg
     1981 acagaaccga gggtttcgac cgaggagagc ttgcggatga gcgcgtggcg gctgaccatt
     2041 tcgcgcatgc cgccagccag gctgatggta accaccgccg gaagaccttc aggcactgcc
     2101 gcgatggcga gcgagactgc aatcatgaac aggttgacca gttcgttgac gaaattcggg
     2161 ttgctgaagg aaaggtgttc gggacccagc aacagccggg caacgccgat ggcgaacacc
     2221 agcgcgcaga caacaatcgc gccgatgccc agagttttcc cgagttggtc caggcgcttc
     2281 atcaatgggg tttgttcttc ttccacggtt tgcagcatgc tggcaatcag cccgagctgg
     2341 gtgttcatac cggtgcttgt gaccacagcc ttgccgcggc cgtaggtgac cagcgtgccc
     2401 atgaaagcgg agtttttccg gtcgccaatc gggatatcgg cttcaagcgc gccttccgca
     2461 ttcttattgg caggcacgga ctcaccggtc agagaagctt cctccacgct caggtttacg
     2521 gcttccaaca ggcggacatc ggctgggatg tagttgccgg cttcgatgaa aacaatatca
     2581 ccgggcacca gcttgggggc tggcaaagac acccggtgcc catcgcgcaa gacttgtgct
     2641 tcgggagccg ccagcttttt cagcgcggcc agggattgtt ccgcgcggga ttcctggatg
     2701 atgcccataa tcgcgttcag gatgacaatc agaaggattg cgccggcttc cacgtaatcg
     2761 cccaacaagg ctgaaatcag cgctgcgcca atcagcagcc agatgacaaa gctgttaatc
     2821 tgcgcccaga gcatttgcag aaaggtcgtg cgcttcttct cctgcagctc gtttgggccg
     2881 tactgcgcca ggcgtttttc ggcttcttcc gtcgtcaggc cggcatccgc cgggacgttt
     2941 agggcgctga gcacttccag cgcgttcagg gcgtgccagg aaacgctgac agattttggc
     3001 tctccacccg gagcgttcgt taaattttca ctcatcgcta ttcctccata aaacatatgc
     3061 ccgcgggcaa ctccaccatt atattctatc tgagggcaaa cttcacgacc tgatattttc
     3121 ttaggaattt atcaggctta cccgcgcggc tggagaattt gcccgggcgg tgcacgggta
     3181 gagcgggctg tgttaaaatc atgccatggg atggaatatt tgcggccatg aaaccgcggt
     3241 cgcattttta acggaacaca cccggccgga aaaactgcgc cacgcctacc tgattaccgg
     3301 tccgcagggc gtcggcaggc gcaccctggc tctggcgttc atcaaagcgc ttaactgcca
     3361 gaatccaccc gcgccggggg agttttgcga ttcctgccag gcatgccggc agataaacgc
     3421 ccggagtttc gcggaccttg cggttctggc gccggatgaa ggccatcagg acctgcgcat
     3481 cgaccaaatc cgcgcggtgc agcagtccct ggcgctcgcg ccttaccagg caaagtaccg
     3541 cgtggtgctc atccaggatt ttcaacgcgc gactgccgcg gcttccaacg cgctgctcaa
     3601 atcgcttgag gaaccgcccg cccgggctgt tttggtactt accgctgacg ctgaagaaaa
     3661 cctgctgccg accattgtgt cgcgctgtca gattgtgcgc ctgcggccgc tcccgatgga
     3721 aaaagctggc gcctggctgg aagaaaagct gcaggtgcca acggaacgcg ccgcgctgct
     3781 ggcgcattta tcctccggca gggtaggagc ggcgctgcgc tttgagcagg accccgagct
     3841 tttgcgcggg tacgaagacg cgctggacca actggaagag ctgctctgct ccagccgacg
     3901 gcagcgcctg caatacgtgg aaaagctgca aaagggcaag ggcgctcagc gcgagctggc
     3961 aggtcaattg gtctccgcct ggctgagctt ttggagggac gcgctgattg tgcgcagcgg
     4021 tgccgatatg cctttggtga accaggaacg gcagggcttt ctgcgggaag ccgccggaca
     4081 gatggagttt cagcgcatcg aggcggtttt agaacagcac gagaaagctc tggcgcaatt
     4141 ggaggcgtac gtcaatccgc gcctgacgct tgagtgtctg ctcctgacgc tcccaatctt
     4201 aaaagcggca tggggaagac aatgagattt gcaaaacctg ccatacagcc cgaacagaaa
     4261 gccaaactga aagacttaat ccgggtcttt ctgcaaattg gcgccaccag ttttggctcc
     4321 cccaccgcgc acatcgcgat gatggaaagt gaaattgtgc ggaaacggaa gtggctgacg
     4381 ggcgaacatt ttttggagct gttggccgcc acaaacctgg tgcccgggcc gaacgcgacg
     4441 gaattggcgc tgcacattgg ttacctgcaa gccggcttcc ctggtttgtt ggcagccggc
     4501 gccagtttca ttctgccttc tttcatcgcc acgctgacac tggcctgggc atacgtccgc
     4561 tacggcgcgc tgccgcaggc ggacgcgatt ttttacgccc tgaacccgct ggtcctggcg
     4621 attgtgataa acacgctctg gaacctgggc aaaggctgcc tgaagagctg gcagacctgg
     4681 ctgattttcg gactggcgct gacgggctct ctgctgggcg tgagcgagct gattgtcctg
     4741 ttcggggcgg gtctgcttgg gtacgtgctg ctggacctca taaaaaaccc tcccaaagcg
     4801 cccgcggcgc tgctggcatt ctttcctttc ccgctgctct tcctgcgttt ctttgcgcag
     4861 gcatccgcct ggctgcaatc ccggacagtc cagctgtttc tgtactttct gcggacaggc
     4921 gcggtactgt ttggctcggc gctggtgctg ttcccgctca tacgggaaga tgtggttaac
     4981 cgcttcgggt ggatgagcca gcagcagctc atcgacgcca tcgcagtcgg gcaaatgacg
     5041 cccggaccgg tcacctcggc ggtcactttc atcgggtaca acgtgggcgg cgttcccggc
     5101 gcgtttgcct ccaccattgg cgtgtttttg ccttctttca tcatcgtggc ggcaatggga
     5161 ccgctgatga aaaagctggc cgattccaag gctgccaagg ctgttttggg aggggtgaac
     5221 gctggcgtga tagccttgat tttcaccatc gcgcttacca tggccaaaaa cgcggtcgtg
     5281 gacgtctgga ccgggctgct gctggcagcg gggctggcgg gcttgttcct gctcaagctg
     5341 accccgtatt ggctggtact ggcgggcttt ctggtcgggc tggcaaaggt gctgttcttt
     5401 tagggtggga ctgcggtaga atcctgtgtc tatgaacgac ttacttgaac tgctcaaccc
     5461 ccagcagcag agcgccgtgg ctgcccctgc cgggccgacg ttggtgctgg ctggtccggg
     5521 ctccggcaag acgcgcgtgc tgacctaccg catcgcctat ctgattggcg tgctgcatgt
     5581 gccggcgtac catatcctcg cggtcacctt taccaacaag gctgccaaag aaatggcggc
     5641 gcgcgtggaa aaaatcctgg gcgcggcagc cgcgggcatc tggctgggca cctttcattc
     5701 cgtttgcgga cgtatattgc gcagggaagc cgacttgctg ccgctgagca aggattttgt
     5761 catcttcgac gcagacgacc aggtgaccct gatgaaagag gtcatccgcg aagccggggt
     5821 ggacgagaaa ttgttccggg cagaatccgt actggacgca atatcccgcg cgaaaaacga
     5881 cctgattagc cccgaagact atcccatcca gaattttcgg gacgaaaaag tgcgtcagtt
     5941 ttacctgcgc taccaacagc tgctggtccg caataatgcc atggactttg acgacatgct
     6001 ggtcttcacc taccagctcc tgagcaccta tccgcagacg cgcagcacct gggcgcataa
     6061 attcgagcac attctggtgg acgaatttca ggataccaac ctctcccagt atgccatttt
     6121 gcgcagcctg gcgcaggagc acggcaatct gtatgtggtt ggcgatgaag accagtctat
     6181 ctaccgctgg cgcggcgcgg attatcgcaa catccagcgc tttcaaagcg acttcaagaa
     6241 cgcccaaacc attttgctgg aacaaaacta ccgctcgacc cagcgcatcc tggacggagc
     6301 catggcggtg attgaccgca acgccaaccg cacgcgcaaa aagctgttta ccgagcaaag
     6361 gcagggcgag aaaatcaagc tgttcatcgc cgcggatgac aaagaggaag ccgaatacgt
     6421 cgtgcaggca gtgaaggacg gaatccgcac ccagaagcag aaccccagct cctttgccgt
     6481 gatgtaccgc accaacgctc agtcgcgcct gctggaggaa tccttccggc gcgagggcct
     6541 cagctaccga ttggtcggcg ctcagcgctt ttacggacgg cgcgaggtga aggatgtgat
     6601 ttccttcctg cgtctgattc acaatcccag ggatgaaatc agcttgcggc gggttatcaa
     6661 cctgcccccg cgcggcattg gcggcaagag cctggaaaag ctggaagaat acgcccgcgc
     6721 ggctgacctg accctgggcg aagccgtcgc gcgccttgct gagccggcag gccgagccgc
     6781 gctggagcag cttggcaagg aaggtctgcg cctggaacag ttcggcaaaa agctgctgtt
     6841 ctggcggcag tacgcggccg ggaacccgcc cgcgtcgctt ttcgaccgcg ttttggaaga
     6901 taccggctac caggaataca tccacgaccg cagcgaagaa gggcaggacc gctgggagaa
     6961 cgtgatggaa ctgcgccagg tcatcctgga gtacgacggg ctgcccggct ttttggaagc
     7021 gatggcgttg gtggccgacc aggacacact gcctgaaacg ctggacgccc cgactttgct
     7081 gaccctgcac gctgccaagg gtttggagtt cgaaaacgta ttcattgtcg ggctggatga
     7141 gatgatattg ccgcacagcc gctcgcgcga cgaccccgaa gcgctggccg aggaacgccg
     7201 cctgctgtac gtgggcatta cccgcgccaa gaaaacgctg accctcaccc gcgcgcagcg
     7261 ccggcgcagc ccctacggca gctatgagga aacccaggcc tcccgctttt tggacgactt
     7321 gcccgaggat ttgctgcaag gcagcgccaa agggctgccc agccggctga acatccaata
     7381 ccaaagcgcc tattcgcgct gggagacgcc cgcctaccgc gccgcgccgc gccaggatac
     7441 gccgcagaac acaaaataca aggccggcat gctggtacgc cacaagacct acgggcgcgg
     7501 ggtggtgaaa gccagccggc ttgagcacgg cgatgagacc gtggaagtgg tgtttgaggg
     7561 cttcggcctg aaagcgctgg tggcctcgct ggcaaagttg gaaatcctct gactttaacc
     7621 gcgcagatcc tgaataaaaa ttcacgctct cctttcggag ggcttttttt atctactccc
     7681 ctggagaaac tctccgggct cgcaaaccca aaccaaaaaa accttttgcg cgcttttacc
     7741 gcgccacatt ttcgggtacg attaaggcat gaaaaccagg cgcgcgcttc tccttgcggc
     7801 ggtcggcgtg cttctgcttg ggctggcctg ccgctatacc gccccgacgc cggcggcatg
     7861 ggctcccagt tctagtgcag agcttgatgc ccgcacgcgc tccgctcgct taaccgcgag
     7921 cgccctggaa gaaacccgct cctcgccgaa ttccccaacc gcgaccagca cgccccttcc
     7981 gcgcccgacc accgcggaag acggaccctg gctgatattc ccggacgcgg caggcgagca
     8041 tttgttcgcg ctggaccggg acagcggcac gctcaccagg attccgctgc cgatgctgac
     8101 cgacccgctg gatatcatcg ggggcagggc gccggacgga gcgcgggtgc tcttacgcgc
     8161 cggcacgccg gataacctgg gtgagctggg cttatatgag cttgtcagcc cctgggaaga
     8221 cccgcgcatg cttaccagcc tgctttccta cgaacttcag gaggatatcc tgcaaaacaa
     8281 cggcatccag gcgcaaatgg ctttgcaggc agtgcagcag ccgcacccgg tcagctggtc
     8341 cgcagatgga aaggcggcag ttttcccggc cgcgctggat gggccctcgg ctgatttgta
     8401 cctataccat cctggagatt ccgctctggt gcgcttgaac cagcgctttc aacaggattt
     8461 ttccccgctg tggtccccgc agcaggattg gattgtcttc caggaggtca acagctacgc
     8521 cacaaccaac gcgtggaagc tctcgctggt cggggcgctg agcatgccaa acggggcggt
     8581 gacgcattac ctgcacgtcc cgcgcgatac cgggtattgg gagcgctacg ttggctggat
     8641 gagcgcgctt tacctgctga cctgcacccc cacggacaac ggcgccgtgg atttgcggct
     8701 gagcaatgtt tcggctggca aaccgcgcgt gattttcgac ggcgctttcg cggaggcagc
     8761 cgttaacccc caaaatgggg agctggcggt agtcgtgcgg gcagagaaca acaaccccag
     8821 gtatccggcg ggcatctatt attcctccaa caacgggacg actttcacgg cgctgctggc
     8881 gggggagtac agaaacctcg agtacagttc tgaaaacggg ctgttcacgg cttccaacgc
     8941 ggtcggggtt gtgcagttcg atagcagcgg cagtgtgttc attttggcgg gtgaggagcg
     9001 cctgggaatt tccccggacg ggcattggct gattggctgg ggcgggaacg gcgcgcgcct
     9061 gtattcgccg caaggttttg aactgcagac tctgacagaa cagagcgtca gcgcgttggc
     9121 atggcaggag gatgccgggg gttttttcct gctggaagaa gacgggctgt attatcttca
     9181 gtttccgctg ctgcaggcgc gtttggtcac acaggatgtg tggcgcgggg atgagagagc
     9241 ctttgtctgg ctgcgttgag ctccggcgaa gccgaacctt ggcgggtgag gcacgtatgg
     9301 caaagtataa tcgcatggaa aaaggagaag tatgagcacc atctttacct ggtatggaca
     9361 cgcaaccatc ggtctggact gtgatggtat caaattggtg gtggacccgt ttttcagtgg
     9421 gaaccctgcc gcggccgtga aggcgcagca ggtgcgggcg gattatctgc tcatctcgca
     9481 tgggcacagc gaccatgttg gcgacgcgct ggatatcgcg cggcgcactg gcgcgaccgt
     9541 ggtggccaac gcggaaatca gcagctggtt ttccggcaag ggcgtgaaga cgcatgccca
     9601 acacatcggc ggaggttttc gttatccgtt tggttacctc aaacttaccc aagcgctgca
     9661 cggctctgcg ctgccggacg gaagcaacgg agggaacccg gccggcttct tgttgagcac
     9721 caacgccggc gagaaaattt acctggccgg ggacacaggc ttgtttggcg atatgcgcct
     9781 gattggcgaa gaagggctgg acctggccgc tctcccgatt ggcgataact acaccatggg
     9841 tccggatgat gcgctgcggg cggtgaagct gctgcagcct aaagtcgtaa ttcctatcca
     9901 ttacaacacc tttccgctca ttcagcagga tgtgcaagcc tggaaaaagc gcgtggaagc
     9961 cgaaaccgga gcgcgcgtgg aagcgctgaa acccggcggg acctatacct taggagcata
    10021 gatgacacag aagagcgaac atcccaaccc aaaacccgga cgcattcccc cggggcagct
    10081 gctgaccaaa cgctttccgg tgctgcacta cggacccgtc ccgagctttg acccgggaac
    10141 ctggaccttt cgggtctggg gcgaggtgga gaagccgctg gcgctcacct gggaagagtt
    10201 ttgcgcgctg ccgcgcacag cgctgaccat ggacctgcac tgcgtgacta cctggagcaa
    10261 gctggatacg cattgggaag gcgtatcgct tgcgactctg cgggagcttg ggctggtggt
    10321 gccgacagcc ggggcgcgct atgtgataca gcacgcggag ttcgggttca ccgccaatct
    10381 gccgattgag gtggctgagg cagataactt tttgctggcg acccattacg aagggcagcc
    10441 gcttacaccg gaacacggct gccctttacg cggcgtgact ggcgcaattc ccgggcgcga
    10501 tgatctcaag gatgtttacc tgtggaaggg cgccaaatgg ctgcgcggtt tggagttcag
    10561 cgcgcacgac cggccgggct tttgggagcg ggctggatat cacaacgacg cggatatctg
    10621 gttggagcag cgcacggcgt agagaaggcg ggctgcattc ctcgtcctcg cgagcaaagc
    10681 gaagcggcct cccggccaaa gctgtcatcc tgaatgctgt gaattcgtca agcgtcgtag
    10741 gttgggtgga gcgccagcgc aacccga
//
LOCUS       DAOQAD010000061        10742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_7074_length_10742_cov_6.055394, whole genome shotgun sequence.
ACCESSION   DAOQAD010000061 DAOQAD010000000
VERSION     DAOQAD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10742
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7074_length_10742_cov_6.055394"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(461..1321)
                     /locus_tag="PKL60_05985"
     CDS             complement(461..1321)
                     /locus_tag="PKL60_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95718.1"
                     /translation="MKNIKIWMLGVSVFLVALLSLLVFWRVNADRGQQLAEQLSQSLQ
                     SERLPLQSVRVANQSPLTLEITLEYSSQDGPERKADILRGRHAVQWHCTQLNREQFQL
                     NSYIVLIQNPSGQQLSWEQNFLYPSDAEDSPEQYSLKLSEANDYLREAFLAGSARVER
                     FALIEEQKDGVPVYTLMIQMIEPDLATLNRELPMAQVERLIRDVNAQKLFPVDICWLE
                     IKGPNGETWVDYLYDYSLSAMNWWIADGVSTEWFPHPAPLPNQEPTATPAARPYPLGT
                     AQPTASSPYP"
     gene            complement(2337..2708)
                     /locus_tag="PKL60_05990"
     CDS             complement(2337..2708)
                     /locus_tag="PKL60_05990"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HNW95719.1"
                     /translation="MAEEVNFICTACPQGCSLTVLMENGEVLGVTGNSCKKGLDYANA
                     ETSDPRRMVATTVKIHNGFHPLLPVYTERPVPKPMIPQVLAEIRKVEVEAPVVMKSVV
                     LENVLGTGINVLASRSMPAKG"
     gene            complement(2717..3994)
                     /locus_tag="PKL60_05995"
     CDS             complement(2717..3994)
                     /locus_tag="PKL60_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW95720.1"
                     /translation="MLAKQIYDVVVVGSGPGGVAAAIEAKKAGADDVLIIERDTELGG
                     ILLQCIHNGFGLELLKEDLPGPGYAQHFIEEMLSLHVEVLLNTMVMDITPQRHLFLSS
                     KEHGYVEIEARAIVLAMGCRERTRAQIRIPGYRPNGIFTAGTAQRWVNVEGYMPGKEV
                     VILGSGDIGMIMARRLTFEGAHVERVLEIQSYLSGLSRNLVQCLQDYNIPLQLNHTIK
                     RILGKNRVEAIEAVQVDEKWNFVPGTEEVIPCDTLLLSVGLIPENELSRKAGVKLDPI
                     TSGPYVDETFQTNVPGIFAAGNVVHVYDLVDWVSEAGFSAGKSAAKYAIALRNAEARY
                     TPLVAGKNVRYVVPHKLDKSTLEKEDIRLQMRVTRPIEQPVWVEVHDGENLITRKAER
                     YVRPGEMVTIVLKAKNYADVAKSSKLTIDINPR"
     gene            complement(3996..5480)
                     /locus_tag="PKL60_06000"
     CDS             complement(3996..5480)
                     /locus_tag="PKL60_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNW95721.1"
                     /translation="MQEKYDYIIIGGGVVGCMLARWLSRYEGKVLLIEKEADVGMGCS
                     SANTAIVHAGYDPLPGSLKARMNVKGNAMWDTLAGELNFAFERRGDYVVAIGDDELPL
                     VHKLMDQGKQNGVPGMLMIDGPEMRAREKHINPETSGALWASTGGICDPFQVTVAAGE
                     NAVMNGAKLLLNTAFQDFIMEGKRIIGVRTNRGDFLGRWVINSAGIYSDEVMHKAGVR
                     PEFIIKPRKGEYYIFDRADVSIDNVLFPVPSHKGKGILVTTTLHENTIAGPNGTIIDD
                     KEDTSTTKEGLAEVADGALKLVPSLNLRYSIANFVGLRPMGNGPCKTPGVVYNNDYII
                     EIPEEVQGLVNLGGIESPGLTSAPAIAEEVVDMLRDAGEKFVAKKDWDPIRPGRPRFR
                     NMSHKDRQLLVEMDPRFGQVICRCENITEGEIIAEIHAPIPARTYDAIKRRTWLGTGR
                     CQGGFDMTRVVTILSRELGISPLEVTKRGTGSEYLFRQTKQVEG"
     gene            6456..6791
                     /locus_tag="PKL60_06005"
     CDS             6456..6791
                     /locus_tag="PKL60_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95722.1"
                     /translation="MAPSQVSPSAWSAASQQLGEPTGSTQRLLISRRSNPDSKQRAGR
                     IFSVQSGALDFSLIAGGGHILARGNVQQETIAGRTPDLIDASASIWEILQARVGNAAH
                     QDSHQAEQN"
     gene            7202..7996
                     /locus_tag="PKL60_06010"
     CDS             7202..7996
                     /locus_tag="PKL60_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HNW95723.1"
                     /translation="MENRSFKEFIGEVFGTFILILLGDGVVANVGLAPRLAGTAYNWN
                     TITIGWAFAVIIAVYVSAGVSGAHLNPAVTLGLAVRRGFPWKKVLPYIVAQMIGAFLG
                     AAAVYLVYRDGLVAAGMPNVWSTGPGSVYDTVFFGADKAGAAGTSYSMLTAIIAEVFG
                     TFMLTWGIAASGDLKNNGLKDNLGGLIVGFTVLAVGLSLGGPSGYSINPARDLGPRLL
                     GALVGTKGLFDGLYWLIPPVLVPAIAGPIGFWLYDVLVSKNLPKKD"
     gene            8071..9072
                     /gene="dhaK"
                     /locus_tag="PKL60_06015"
     CDS             8071..9072
                     /gene="dhaK"
                     /locus_tag="PKL60_06015"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HNW95724.1"
                     /translation="MKKLINKPEDVVLEELQGIELAHPDIVKVFYDPNYIVRADAPVK
                     GKVGLVSGGGSGHEPMHGGFVGMGMLDAACPGAVFTSPTPDQMLEATKAVNGGAGVLH
                     IVKNYTGDVMNFEMAADLAKEEGIEVQSVVVDDDVAVKDSTWTAGRRGVGLTVLMEKI
                     VGAAAEEGRSLKEVADLARKVNAQGRSMGMALTSCVVPHVGKPSFDLPEDEMEIGIGI
                     HGEPGRTRMKLQPADKVTEMLLTPIVEDLPFKAGDEVLLFVNSMGGTPLIELYIIYRK
                     AVQMLEAKGIKVTRHLIGPYITSLEMAGASITLLKLDADLTKLWDAPVKTAGMRWGI"
     gene            9095..9769
                     /gene="dhaL"
                     /locus_tag="PKL60_06020"
     CDS             9095..9769
                     /gene="dhaL"
                     /locus_tag="PKL60_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HNW95725.1"
                     /translation="MDVPLQNLSYEVNVAISRENVLEWIRTFSQVIDENNVYLTDLDR
                     EIGDADHGANMNRGFKAVMTKLPSMETSDITGILKGVGMTLLSSVGGASGPLYSTVFM
                     QLSKSFAGKEQLELADWCAGWEAAEQGVQMRGKAIPGDKTMVDAMQPALEALKEAVAQ
                     NLPIGVGLRNSADAAHQGMLATIPLVARKGRASYLGERSANHQDPGATSTYFLLDSAA
                     KTWADC"
     gene            9812..>10742
                     /locus_tag="PKL60_06025"
     CDS             9812..>10742
                     /locus_tag="PKL60_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNW95726.1"
                     /translation="MAKYVGAVDQGTTSTRFMIFDHKGAVVGYDQKEHEQIYPKPGWV
                     EHNPLEIWQATQEVIKGALDKLHIDIKEIAAVGVTNQRETAVVWEKATGKPVYNAIVW
                     QDTRTDQICNELGKVGGQDKYRPKVGLPLATYFSGMKVKWILDNVPGVREKAEKGEIL
                     FGNIDTWVIWNLTGGPKGGVHVTDVTNASRTFMMNLETLDWDPDMLKDLSVPRAMLPA
                     IKPSSEVYGYCQNSGLDGIPVAGDLGDQQAALFGQTCYDPGEAKNTYGTGCFMLMNTG
                     TKPIQSKNGLLTTLGYKIGKEPAVYALEGSIAIT"
BASE COUNT         2364 a         3042 c         2903 g         2433 t
ORIGIN      
        1 agcgactgaa gcaatctccc cctatgccgg ctgggagact gcttcgtgcc tcgcggtaac
       61 gcgtgttgtc attctgggac gcaggcgaag aatccgtccc ttcggaaaac aagatccttc
      121 gcaggctcag gatgacaaat acgtatacag ggtcagggtg acaggcattt gctttcacct
      181 cgctcggcta agatgccagc acggagatcg cttcgttcct caaggggacg ctcgattcgt
      241 cattgcgatg gagcgccagc gactgaagca atctccccct atgccggctg ggagactgct
      301 tcgtgcctcg cgttgaggaa atattgtcag tctgggccgc aggcgaagat tccgatcttt
      361 acagccagat ccttcgcagg ctcaggatga caagcttaca aggatggcaa acattcaccc
      421 ccccttcacg cgttaccggc atgcacgcga acgcaaaggc tcaaggatac gggctgctcg
      481 cggtcggctg cgcggtgcca agcggatacg gcctggcagc cggcgttgca gtcggttcct
      541 ggtttggcag cggcgcggga tgcgggaacc actccgtgct gactccgtcc gctatccacc
      601 aattcatcgc ggacaggctg taatcataaa ggtaatccac ccacgtttcg ccgttcggcc
      661 ccttgatttc caaccagcag atatccaccg ggaagagctt ctgcgcgttg acatccctaa
      721 tcaggcgctc aacctgtgcc ataggcagct cgcggttgag tgtcgcaagg tcaggctcga
      781 tcatctggat catgagggtg taaacaggca cgccgtcttt ttgttcctcg atcagtgcga
      841 agcgctccac ccgcgcagaa cccgccagaa aagcctcgcg caggtaatca ttggcttcag
      901 aaagtttcag gctgtactgc tcagggctgt cttcagcgtc gcttgggtag aggaagtttt
      961 gctcccagct gagctgctga ccagaggggt tctgaataag cacgatgtag ctgttgagct
     1021 ggaattgctc ccggttgagc tgcgtgcaat gccactgcac cgcgtgccgg ccccgaagga
     1081 tatccgcttt gcgctccggg ccgtcctgcg agctgtattc aagggtgatt tccagggtta
     1141 gaggtgactg gttcgcgacg cgcacgctct gcaaaggcag cctttcagac tgcagagatt
     1201 ggctgagctg ttccgcaagc tgctgacccc ggtctgcgtt cacgcgccag aagaccagca
     1261 aggagagcaa tgctacaagg aagaccgata ctccaagcat ccagattttt atatttttca
     1321 tagccaattt ctccatttag tttgaaaaaa taacatgaag aaactgctga tatgtcagct
     1381 cgcgatatgt caggtaggtg gattcatcct caatcgctat taagtaagga aattgttcga
     1441 accaattccc ttggtcgaac aagtaatagt tattacttga atctcgatat gatgcgtttt
     1501 caaattgcgt gaataattcg attcgatggt cttcatgaat ctcggacatt acctcaataa
     1561 cttcgggagc atacacaatt tctcgacacc caatacctag gttattgata tatgcacacc
     1621 aaacatcatc tcctacatta atgattttgt attcgacctt tgtgccccaa tcctgattac
     1681 cttgatggta aatctcgcaa ttggtaatag gtgagtggta ttccacgtat ttctgaggga
     1741 gttgaaagct cgcatagtat cgccagccag cttgaatcca atatggcaat ggaagtgata
     1801 tccaatttga ttgaccatta ttgaaaacat taattgggta accgggcgtg tagatatcga
     1861 cctttccacc atatgctatg taataggaat ttccaatgta aaaagctgat gatgctgttg
     1921 attggggtaa taagagaata acgaacaaaa tcgtacatga agtccaattt tcataatatc
     1981 cctcctaaat ttttttctct aatagtagtg tacaacatat ccatccaaaa accaaggaaa
     2041 atgaagaaat ataaaaaggt aaaggatgct ttctccgaac gttatagaat ttaccaggaa
     2101 cgggaattga gtttaagagg ctaattgtgc ttatagctta tggtatgcag gttgggttac
     2161 cctccggaca atcattcact acttcttgca tggtatcgtt ccatccaagc taccactacc
     2221 cggtctgaag gctggcaagc acatagatcc ttctaaggct caggacgacc atctgcctca
     2281 ccccaacaaa agagcggagc cgcgcgcggc tccgcttcct cacatccaaa cacacactac
     2341 cccttggcgg gcatgctgcg gctggcaagc acattaatcc ccgtgcccag cacattctcc
     2401 agtacgaccg acttcatcac cacaggtgcc tcaacctcca ccttgcgaat ctccgccaat
     2461 acctgcggga tcatcggctt gggcacaggc ctttcggtgt aaaccggcag cagcgggtgg
     2521 aatccattgt gaattttcac cgtcgtcgcc accatgcggc gggggtccga ggtttcggca
     2581 ttggcgtaat ccaggccctt cttgcaggaa ttgccagtca ctcccaacac ttccccgttt
     2641 tccatcaata cggtcagact gcagccctgc ggacaggcgg tgcaaatgaa attaacttct
     2701 tcagccattt ttcctcctaa cgcgggttga tatcaatggt cagcttgctg gatttggcaa
     2761 cgtccgcgta gttcttggcc ttcaggacga tggtcaccat ctcaccaggg cggacgtaac
     2821 gctccgcttt gcgcgtgatc aggttctcgc cgtcatgcac ttccacccac accggctgtt
     2881 caatcgggcg ggtgacgcgc atctgcaggc ggatatcttc cttttccagc gtgctcttat
     2941 ccagcttatg cggtacaaca tagcgcacat tcttgccggc gacaagcggc gtgtagcggg
     3001 cttcggcgtt gcgcagcgcg atggcgtact tcgccgcgct cttgccagcc gagaagccgg
     3061 cctcggacac ccagtccacc aggtcgtaga cgtgcaccac gttgccggcc gcgaagatgc
     3121 cgggcacatt ggtttgaaag gtctcatcca cgtacggccc gctggtaatc gggtccagtt
     3181 taacaccggc cttgcgtgaa agctcgtttt ccggaatgag gcctaccgaa agcagcaggg
     3241 tgtcgcaggg gataacttcc tccgtgccgg gcacaaagtt ccatttttcg tccacctgca
     3301 ccgcttcgat ggcttccacg cggttcttgc ccagaatgcg cttgatggtg tggttaagtt
     3361 gcaaggggat gttatagtcc tgcaggcact gcaccaggtt tcggctgagg ccggagaggt
     3421 acgactggat ttccagcacg cgttccacat gcgcgccttc aaaggtcagg cggcgggcca
     3481 taatcatgcc gatatcgccc gagccgagga tgacaacctc tttgccgggc atatagcctt
     3541 ccacgttcac ccaacgctgc gcggtgccgg cggtaaagat gccgttcggg cggtatcccg
     3601 ggatgcggat ctgggcgcgg gtgcgttcgc ggcagcccat cgccaaaaca atcgcgcggg
     3661 cttcaatttc cacatacccg tgttctttgc tggaaaggaa caggtggcgt tggggcgtga
     3721 tatccatcac catcgtgttc agcagcactt ccacgtgcag gctcagcatt tcctcaatga
     3781 aatgttgggc gtagccagga ccgggcaggt cctctttcaa cagctccaga ccaaaaccat
     3841 tatggatgca ctgcagcaga atgccgccca gttcggtatc gcgttcgatg attaacacat
     3901 catccgcgcc ggctttcttg gcttcaatcg ccgccgcaac gccgcccggg ccgctgccaa
     3961 ccacaacaac atcgtagatt tgtttcgcta acatctcagc cctccacctg ttttgtctgt
     4021 cggaataaat attcggaacc ggtgccgcgc ttggtcactt ccaggggaga gatgccgagt
     4081 tcgcgggaaa ggatggtcac cacgcgggtc atgtcaaaac cgccctggca gcggccggtg
     4141 cccagccagg tgcgccgctt gatggcgtcg taagtccgcg cggggattgg cgcgtgaatt
     4201 tcggcgataa tttcgccctc agtgatgttc tcgcagcggc agataacctg cccaaagcgg
     4261 gggtccatct ctaccaacag ttggcggtcc ttatgggaca tattgcggaa gcgcggccga
     4321 ccgggtcgga tggggtccca atcctttttg gccacaaact tctcgccggc gtcgcgcagc
     4381 atgtccacca cctcttcggc gatagccggc gctgaggtca gcccggggga ttcgatgccg
     4441 cccaaattca ccagaccctg cacttcttcc gggatttcga tgatgtaatc gttgttatac
     4501 accacgccgg gcgtcttgca ggggccgttg cccatcggac gcaggcccac gaagttggca
     4561 atggaatagc gcagattcag cgagggaacc agtttgagcg cgccgtccgc cacctctgcc
     4621 agcccttctt tggtggtgga ggtgtcttcc ttgtcatcga taatcgtgcc gttcggtccg
     4681 gcgatggtgt tctcgtgcag tgtggtggtc accaggatgc ccttgccctt gtgggagggc
     4741 accgggaaga gcacattgtc gatggacaca tccgcgcggt cgaaaatgta gtactcgccc
     4801 ttgcgcggct tgatgatgaa ttccgggcgc acgccggctt tgtgcatgac ctcatcggaa
     4861 tagatgcccg cggagttgat gacccaacgt ccgaggaaat ccccgcggtt ggtgcgtacg
     4921 ccgataatgc gttttccttc catgatgaaa tcctggaaag cggtgttcag cagcagcttc
     4981 gcgccgttca tcacggcatt ttcgcccgcg gcaaccgtaa cctggaaggg gtcgcagata
     5041 ccgccggtgg aagcccagag cgcgccgctg gtctcagggt tgatgtgctt ttcacgcgcg
     5101 cgcatttccg gaccgtcaat catcagcatc ccgggcacgc cgttctgctt gccctggtcc
     5161 atgagcttgt gcaccagcgg cagctcgtcg tctccgattg ccactacata gtcgccgcgg
     5221 cgctcaaagg cgaagttgag ttcaccagcc agcgtgtccc acatggcgtt gcctttcacg
     5281 ttcatgcggg ctttcaggga gccgggcagc gggtcgtaac cagcgtggac aatggctgtg
     5341 ttggcgctcg agcagcccat accgacatcg gcttccttct cgatgagcag caccttccct
     5401 tcgtaccggg aaagccagcg ggcgagcata catccaacca cacccccgcc gatgataatg
     5461 taatcatatt tttcctgcac acttacctcc aattaaggtt gttcaaaaaa tgcacgtcat
     5521 taaaaaaagc agctggtttg ctttcccgtg gaacgcagcc agcggcatga ttccctttct
     5581 ggtaaagaac aggcgctcaa tccaagcaat ccgcattgga aagctgcctg agctaaaggt
     5641 aaagaaaaag tgtgcgctgc gggcactcaa catttgaccg gaaaactgtc ggacgtccaa
     5701 ttgcctttgc attcttgagt cgaatcccga aaacccgctg ttttctttgg tgagttggcg
     5761 tgattatatc agcatattgc cgttttgcgg cattatcttc attatctatc cacatccacc
     5821 ataaatttca attttcttta atcccatgca ttctttcagg cacattaacc ggaacgaggg
     5881 aatctgcgaa gaaatcatta ccctcgccgg gtctgtcttc cttgccgcgc tgaattttct
     5941 gaccgccgga ctgtacctcg ctgaccggat gaacaggctg ctgtacctcc gggccagctg
     6001 ctgcgcggcc tgctctgcgt tttgccaggc atccaactct cccaagcctt tccctcggtc
     6061 ttgaggctgc gcgccgcttc gcagcgcccg gacaatcaga ctcatgtcgg ccagaggaaa
     6121 gagctgggga gcgctgccgg atttctgctg cgctgcccgc caggcgtggg cttccccagg
     6181 cgccgggacg cagccagccc cgcgctggaa aacgcggagc ccgcctattc ttccgaccgg
     6241 atggacacgc tttcgcgcga gtacccaatc gcccccggga gggtggttca actggctttc
     6301 caacggcggc ggtatgccgg tcgccgctgc ttgtgcggtc ccgctgcata tccccgcctt
     6361 cggctccgtt gccggcgcgg atgtttgggc ctgggggaac agcttgccca cgcaggtagt
     6421 tcccgctttc cacttccacg aggatgctga ccctgttggc gccttcccag gtcagtccgt
     6481 ctgcctggtc cgccgcttct caacagctgg gtgagccaac cggcagcaca caacggctgc
     6541 tcatcagccg ccgctccaat cccgacagca aacagcgcgc agggagaata ttttcagtac
     6601 agagcggcgc actggatttc agcctcatcg ctggtggcgg gcatatctta gcgcgcggga
     6661 atgtgcagca ggaaaccatt gccggcagga ctccggatct gattgatgcc agcgcatcca
     6721 tttgggaaat cctccaggcg cgcgtgggaa acgcggcaca tcaggattcg caccaggctg
     6781 agcagaactg atatgcaatt ggtacaaaaa acccgcaaat tctacgaaat ttgtataacc
     6841 cttttgactc tgcctgttca atatgctata ctgagagcaa ttcggaaaac tccagccgcc
     6901 catttcctgg gtgttatagt ttactggaag ttgtgttgtg gatcgcagtg gtaggaggaa
     6961 tgcgggcagg accagcttgc aatttaaata gaccgcgttt tacatgtcgg cctcgtggag
     7021 ttgtctctca ccccataatc tccacacaga atgatccata gtctatcaac ttcgtttaag
     7081 taatttggag taatttttat gaaaggagga gccaggaata agacaaaacc gatagcgaac
     7141 gttcaatccc aaactttaaa gatcattttt ttaacatatt cgaaaaggag attttgtaaa
     7201 gatggaaaat agatcgttca aagagtttat tggtgaggtg tttggtacgt ttatcctcat
     7261 tctgcttggt gacggtgtcg ttgccaatgt tggtttggct ccccgccttg caggtaccgc
     7321 ctacaactgg aacaccatta ccatcggctg ggccttcgcg gttatcattg ccgtgtacgt
     7381 ttccgctggt gtctccggcg ctcacctgaa tcctgccgtg accctcggtc tggcagtgcg
     7441 ccgcggtttc ccgtggaaaa aggtcctccc ctatatcgtt gctcagatga ttggcgcttt
     7501 cctcggcgct gccgctgtgt acttagtgta ccgcgacggc ctcgtggctg ctggtatgcc
     7561 caatgtgtgg tcaaccggcc ccggctctgt gtacgataca gtcttctttg gcgctgacaa
     7621 ggccggcgct gcgggtacct cctacagcat gctgaccgcc atcatcgcgg aagttttcgg
     7681 caccttcatg ctcacctggg gtattgctgc ctccggcgac ctgaagaaca acggcctgaa
     7741 ggacaacctg ggcggcctca tcgtgggctt cacagtgctt gcggttggtc tgtccctcgg
     7801 cggccccagc ggctattcca tcaatcctgc ccgtgacctc ggcccccgtc tgttgggcgc
     7861 cctggttggc accaagggcc tctttgacgg cttgtactgg ctgatcccgc cggtgctcgt
     7921 gcccgcaatc gccggcccca tcggcttctg gctgtacgac gtgctcgttt ccaagaacct
     7981 gcccaagaaa gactaattcc tgatttcagt aaacagccgc tgcatctgca tgcttacctg
     8041 aacttttagc ttagaaagga gactctgctt atgaagaaat taattaacaa gcccgaagat
     8101 gtcgttttgg aagagcttca gggtattgag ctggctcacc ctgacatcgt aaaggttttc
     8161 tacgatccaa attacatcgt ccgtgccgac gcccccgtga agggaaaagt aggccttgtc
     8221 tccggcggag gcagcggcca cgaacccatg cacggcggtt ttgtgggaat gggaatgctt
     8281 gacgccgcct gcccgggcgc cgtcttcacc agccctaccc ccgatcaaat gctcgaagcc
     8341 accaaggccg tcaacggcgg tgctggcgtc ctgcacattg tcaagaacta taccggcgat
     8401 gtgatgaact ttgaaatggc tgccgacctg gcaaaggaag aaggcatcga agtgcagtcc
     8461 gtcgtggtgg atgacgatgt ggctgtaaag gacagcacct ggactgccgg acgccgcggc
     8521 gttggtctga ctgtgctgat ggaaaagatt gttggcgccg ccgccgaaga aggccgctct
     8581 cttaaggaag ttgccgacct ggcgcgcaaa gtcaacgctc aaggccgcag catgggcatg
     8641 gcgctcactt cctgtgttgt cccgcatgtt ggcaaaccct cctttgacct gcctgaagat
     8701 gagatggaaa ttggcatcgg catccacggc gagcctggac gcacccgcat gaagctgcag
     8761 cccgccgaca aggtgaccga aatgctgctc accccgatcg tcgaggacct gcctttcaag
     8821 gctggcgatg aagttttgct gtttgtcaac agcatgggcg gcacccccct gatcgaactc
     8881 tacatcattt accgcaaagc ggtgcagatg ctggaggcga agggcattaa agtaacccgc
     8941 cacctgatcg gtccctatat caccagcctg gaaatggctg gagcctccat caccctgctg
     9001 aaactggacg ctgacctgac caaactctgg gatgcgcctg tgaagactgc cgggatgcgc
     9061 tggggaattt agtaagttgt ccaatggttc tggagtggat gtcccactcc agaacctatc
     9121 ctatgaggtt aatgtggcca tatcaagaga gaatgtttta gagtggattc ggactttttc
     9181 ccaggtcata gacgaaaaca atgtgtatct gaccgacctc gaccgtgaaa tcggggacgc
     9241 ggatcacggc gccaatatga accggggatt taaagcagtc atgaccaaat tgccatccat
     9301 ggaaacaagc gacatcaccg gcatcctgaa gggtgtgggg atgacgctgc tgtcctcagt
     9361 aggcggcgcg agcggtcctt tgtattcaac ggtttttatg cagctcagca aaagctttgc
     9421 gggcaaagaa cagttggagc ttgcggactg gtgcgcgggc tgggaagcgg ctgaacaagg
     9481 cgtgcagatg cgcggaaaag cgatccccgg ggataaaacc atggtggatg cgatgcagcc
     9541 cgctttggaa gcgctgaagg aagccgtggc gcaaaacctg ccaatcgggg tggggctgcg
     9601 caattccgcg gatgccgccc atcagggtat gctggcaacc attccgctgg tcgcgcgcaa
     9661 agggcgcgcc agttatttag gcgaacgcag cgcgaaccat caggatcccg gcgcgacttc
     9721 cacatatttt ttgcttgatt ccgccgcaaa aacatgggca gattgttaat gcccgtaaac
     9781 tgtcaaacta attagcaaaa aaggagaaat aatggcaaag tacgtaggag cagttgatca
     9841 gggtactacc agcacccgct ttatgatttt tgatcacaaa ggcgcagtgg taggctatga
     9901 tcagaaagaa cacgaacaga tttacccgaa acccggttgg gttgagcaca atcccctcga
     9961 gatctggcag gctacccagg aagttatcaa aggcgctctg gacaagctcc acatcgacat
    10021 caaggagatc gcagcagtcg gcgtcaccaa ccagcgcgaa accgccgttg tctgggaaaa
    10081 agccaccggc aaacccgttt acaacgccat cgtctggcaa gacacccgca ccgaccagat
    10141 ctgcaacgaa ctcggaaaag ttggcggtca ggacaagtac cgccccaagg ttggtctgcc
    10201 tctggcgacc tacttctccg gtatgaaagt caagtggatc ctggacaacg ttcccggcgt
    10261 ccgcgaaaaa gctgagaagg gcgaaatcct cttcggcaac atcgatacct gggtgatctg
    10321 gaacctcacc ggtggcccca agggcggtgt gcacgtgacc gatgttacca acgcctcccg
    10381 aaccttcatg atgaatctgg aaaccctcga ctgggatccc gatatgctga aggacctcag
    10441 cgtcccgcgc gccatgctcc cggcaatcaa accctcctcc gaagtgtatg gctactgcca
    10501 gaactccggt ttggacggca tcccggtagc aggcgacctg ggcgaccagc aagccgcgct
    10561 ctttggccag acctgctatg acccaggcga agccaagaac acctacggaa ccggctgctt
    10621 catgctcatg aacaccggca ccaaaccgat ccagtccaag aatggtctgc tgactactct
    10681 gggatataag attggcaagg aacccgctgt gtacgccctg gaaggctcca tcgccatcac
    10741 cg
//
LOCUS       DAOQAD010000062        10683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_7142_length_10683_cov_4.967350, whole genome shotgun sequence.
ACCESSION   DAOQAD010000062 DAOQAD010000000
VERSION     DAOQAD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10683
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7142_length_10683_cov_4.967350"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKL60_06030"
     CDS             complement(<1..212)
                     /locus_tag="PKL60_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95727.1"
                     /translation="MGTRDGFQGGLAEGVAAGFGDVRVLRRDVQRLWQFEALLARHQP
                     ALLVTMLNRNNEQILLAQGFDRFVNVL"
     gene            150..416
                     /locus_tag="PKL60_06035"
     CDS             150..416
                     /locus_tag="PKL60_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNW95728.1"
                     /translation="MPKTCCNAFRKTALEAISRAQEVNPVIVTGFLAEGCVLSTVRQA
                     RDLDLTVIILRNRIDSGSEGRRKFVEKIRDVFSDRALKKMLEQF"
     gene            complement(422..1480)
                     /gene="mnmA"
                     /locus_tag="PKL60_06040"
     CDS             complement(422..1480)
                     /gene="mnmA"
                     /locus_tag="PKL60_06040"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNW95729.1"
                     /translation="MKKVLVALSGGVDSSVAAALLVEQGYEVVGATMRLWSASGAEQE
                     NRCCTPDSRSLAARLADKLSIPFTILDETETFRKIVVQQFLDGYARGETPNPCVTCNR
                     FLKWGALWDYAKSIGADYLATGHYARVLAGPEGLFELYKGKDPGKDQTYFLSMLTQEQ
                     LAHTLFPLGELLKTDVRGIAHARGLPAADQPESQDLCFLGERDYRDFLAEYAPETVQP
                     GPITDRSGKLLGQHQGLAFYTIGQRKGLPAAAQALYVWQKDIPNNRLVVCPAEELGAS
                     TLRVEQLNWISGAAPTDEFQAEVKIRFRAIPANGLVIPQPNGSALVRFQKPIRDITPG
                     QLAVFYLGEQVLGGGWIA"
     gene            complement(1585..2256)
                     /locus_tag="PKL60_06045"
     CDS             complement(1585..2256)
                     /locus_tag="PKL60_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95730.1"
                     /translation="MISVRNVLVQAGGRVILDIPLLDFEEGKRYGLIGENGSGKTTLL
                     RVLAGTLQPTHGEVSRQNPDGLGYMPQSPYAFSFSVLRNVEMALENAPDRREKAACAL
                     EAVGMQNFLTARGNKLSGGEAQRMAVARMIAKPRQLLLLDEPTSSTDIRGTDSIENTL
                     LDYTRSTRCTLIFSTHSPAQALRLAEEVIFLDQGRILEKGSAREVLETPQTESARLFL
                     QHWRI"
     gene            complement(2253..2939)
                     /locus_tag="PKL60_06050"
     CDS             complement(2253..2939)
                     /locus_tag="PKL60_06050"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95731.1"
                     /translation="MDVIREIFGLLFGQDPELRQIIGVTLRMSTLSTLISALLGIPLG
                     VIIGSTDFKAKRFINRVLHTLMGLPPVVAGLIVFLFLSRSGPLGTLKLLFTVTAMVIA
                     QIVLITPIMTGLTANIVSVKAPLIHETAAGIGLRPFQRMLYIIYECRVQFISTVLTGF
                     GRAIAEVGAVQMVGGNVQFKTRVMTTAIMMQTNMGHFEFAIALGVVLLGIAFLINSLV
                     SRIQESAGLA"
     gene            complement(3047..3865)
                     /locus_tag="PKL60_06055"
     CDS             complement(3047..3865)
                     /locus_tag="PKL60_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNW95732.1"
                     /translation="MKKLCVVLIMGVLILSLTACATKIENPVIRLSTTTSVNDSGLMA
                     YLQPVFEKDTGYTLEITSAGTGAAIEKGRTGDADVLLVHAKASEEEFINEGFGEVRVP
                     FMYNFFVIVGPEGDPAGVKGSTTAAEAFKKIADAGATFVSRGDDSGTHKAELKIWKSL
                     ELDPTGQPWYVSVGDSMGKTLTVGNEMQGYVFTDKATYLAHDNTLSLLLEETDDMKNT
                     YSLIAISPQRFADTNYAGAQAFIEWMTSEKAADLIAKYGIEEYGEQLFFILATK"
     regulatory      complement(3857..4005)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     assembly_gap    4306..4405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4686..7142)
                     /gene="leuS"
                     /locus_tag="PKL60_06060"
     CDS             complement(4686..7142)
                     /gene="leuS"
                     /locus_tag="PKL60_06060"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNW95733.1"
                     /translation="MIDPTSIPPYKPEEIEPKWQARWAADKLYETADHPEKQKFYALT
                     MLPYPSGDLHIGHWYAMTPSDARARFKRMQGYNVFFPIGFDAFGLPAENAAIKRRIHP
                     KTWTYANIARMRGQLRSMGNMFDWNSEIISSDPKYYRWTQWFFLQFFKHNLAYQKEAP
                     VDFCPNCNTTLAREQVQGEDRHCERCGTPVIRKDLRQWLFRITNYAEELLDFSDIDWP
                     DTIKTLQTKWIGKSKGAFVTFTSEQGDPIEVFTTRPDTLWGVTFMVLAPEHPLVEKLT
                     TAEQAEIVRAYQEQAKRQSDIQREAVDRTKTGVFTGAYALNPVSGERVPVWTADYVLM
                     GYGSGAIMAVPAHDQRDFEFARQYGLPIRVVIQPEGSPEQDPAEMTAALEAHGAMVHS
                     GPLSGTPEEKTMEAAIAYIERLGVGKGAVTYRLHDWLISRQRYWGAPIPIIYCPHCGT
                     LPVPEEQLPVLLPDDVEWLPTGESPLKLHPTWRFTTCPVCGGPAERETDTMDTFMCSS
                     WYFMRYLSPDYDTYPFDPALYRKWMPVDTYTGGSEHATMHLMYVRFFHKACRDCGITI
                     GPEPMLQLRNQGMVLGEDGEKMSKSRGNVIAPDDLVSRYGADTVRSYLTFFARWEVGG
                     PWNSSGIEGNSRWLRRVWAMLLEPAQTDASPETLRALRRKTHQVLRAITYDYENFEFN
                     TIVSSLMELLNEMGRAKNAGAWGSPEWNECADIYLRMLAPLCPHIAEELWERLGKPYS
                     IHTQSWPVVDEAAARDEEITLVVQVNGKLRDRILVPPGITDAEAQEKALASEAVRAAL
                     EGKPVRKVIIVPGRLVNIVA"
     assembly_gap    7726..7825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7846..10062
                     /locus_tag="PKL60_06065"
     CDS             7846..10062
                     /locus_tag="PKL60_06065"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HNW95734.1"
                     /translation="MTEEPKNDDEMTQKIYHSSMHHANMEHEHHMHEMTGMDHDHSKM
                     PASHDAHAGHEMNMHEMDMGGHGDHNMPMEHGGHEDHSAHVDHSGHEQMFRRKFWVSL
                     ILSLPVLLFSQGFQALLGFSMPVFPCSRWITPIFAVIIFVYGGLPFIKMAVPELRNRQ
                     PGMMTLISLAIIVAFVYSVASLFLANQMDFFWELATLIVIMLLGHWIEMRSIRQASGA
                     LNALAKLMPDSAERIDANGQTTKVVVSDLKSGDRLLVRPGSSIPADGVVEDGESAVNE
                     SMITGESKPVHKHPGEKVVGGSINGDGSLRIRVTATGDQTALAGIMRLVQQAQMSKSR
                     TQLLADRAAGWLFYIALGTAVITAIAWTIGVGFNVTVIERVATVLVIACPHALGLAIP
                     LVVAITTAMGANNGILVRNRLALEAAREINTVIFDKTGTLTEGRFGVVGIETLDGIKP
                     EDALALTAAVEGDSEHTIAQGIRRSAQQKGLQLPVVQDFEAIKGRGIHAKMSGHDVYV
                     GGPRLLEMLTITLPESLSIFDQAASAKGQSVVHLVQDGKPLASLALADVVRPESHEAI
                     RRLHEMGVQVAILTGDSQAVAKAVAGELGIDTYFAEVLPENKDQKVIELQKQGRRVAM
                     VGDGVNDAPALTRADVGIAIGSGTDVAVESAGIILVKSNPLDVVKVIELSRASYRKMR
                     QNLIWAAGYNVVAIPLAAGVLAPWGILLSPAVGAILMSLSTVIVAINAQLLRTKHL"
     gene            complement(10354..>10683)
                     /locus_tag="PKL60_06070"
     CDS             complement(10354..>10683)
                     /locus_tag="PKL60_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95735.1"
                     /translation="PKGMTYFEFVADRIDAAKTVEPSRCGWGMMLSLATLGPIYSIVY
                     TEVGIHPDGALARGTAPDPDIPKDVAHAKWYEVPGIWWNTVERLSWTMVGKQAAFGCK
                     FRKVDGL"
BASE COUNT         2218 a         2833 c         3139 g         2293 t
ORIGIN      
        1 agtacattaa cgaagcgatc aaatccttgc gcgagaagaa tctgctcatt attgcgattc
       61 agcatcgtga cgagcagggc aggctggtgc cgggctagga gggcttcgaa ctgccacaaa
      121 cgctgaacat cacgcctgag gacgcgcaca tccccaaaac ctgctgcaac gccttccgca
      181 agaccgccct ggaagccatc tcgcgtgccc aagaggtgaa tcctgtcatc gtgaccggct
      241 tcctggcgga aggctgcgtg ctttccaccg tgcgccaggc gcgtgacctg gacctcaccg
      301 ttatcatcct gcgcaatagg attgactctg gctccgaagg gcgccgcaaa ttcgtggaaa
      361 agatccgcga tgttttcagc gaccgagcac taaagaagat gctggagcag ttctgacttg
      421 gttaagcaat ccaaccgccg cccaatacct gttcccccag gtaaaaaaca gccagctgcc
      481 caggggtgat atcccttatg ggcttctgaa agcgtaccaa cgcgctgccg ttcggctgcg
      541 ggatgacaag cccattggcg ggaatagcgc ggaagcggat ttttacttcc gcctggaatt
      601 catctgtcgg ggcggcgccg gatatccaat tcagctgttc aacgcgcaga gtgctggcgc
      661 ccaattcttc cgccgggcaa acgaccagac gattgttcgg gatatctttt tgccatacgt
      721 acagcgcttg cgccgcggcg ggcaggcctt tccgctgacc gatagtatag aacgccaagc
      781 cctggtgctg ccccagcagt ttaccgctgc ggtcggtgat gggaccgggc tggacagttt
      841 ccggcgcgta ctccgccagg aaatcgcgat aatcccgctc gcccaaaaag cacaggtcct
      901 ggctttcggg ctggtctgcc gcgggcagcc cgcgcgcgtg ggcgatgccg cgcacatccg
      961 tcttcagcag ctccccaagc ggaaagagcg tgtgggcaag ctgttcctgg gtgagcatgc
     1021 tgaggaaata ggtctggtct ttgcctgggt ccttgccttt atagagttca aacagcccct
     1081 ccggcccggc aagcacgcgg gcgtagtgcc cggtggcgag gtaatccgcg ccgatggatt
     1141 tggcgtagtc ccacagcgcg ccccacttga ggaagcggtt gcaggtgacg caggggttgg
     1201 gggtttcacc ccgcgcatag ccgtccagga actgctgcac cacgattttg cggaaagttt
     1261 ccgtttcatc caaaatggta aaggggatgg agagtttatc cgccaacctg gctgccagac
     1321 tgcgtgaatc tggtgtgcag cagcggtttt cctgctcagc gcccgaggcg ctccacaggc
     1381 gcatggtcgc gccaacgact tcataaccct gctcgacgag cagagctgcc gcgaccgaac
     1441 tatccacgcc gccgctgaga gcgaccaaaa cttttttcat acgattttcc cattttttct
     1501 tgttgcgcgg caggtagccg cgctgttcca ttataaacgg gcggcgggag agagggaaaa
     1561 ttaggattgt acgcggggat gagcttagat gcgccagtgc tgcaggaaca ggcgcgcgct
     1621 ttccgtttgg ggggtttcaa gcacttcgcg ggctgaacct ttttccagta tcctgccctg
     1681 atccaggaaa atgacttcct ccgccaggcg cagtgcctgc gcgggcgaat gagtggaaaa
     1741 aatcagggtg cagcgcgtgc tgcgcgtgta atccagcagg gtgttttcaa tggaatccgt
     1801 gccgcggata tccgtggagg aggtcggttc gtccagcagg agcagttggc gcggcttggc
     1861 aatcatgcgc gctaccgcca tgcgctgggc ttccccgccg gagagcttat tcccgcgcgc
     1921 cgtgaggaag ttttgcattc ccaccgcctc aagcgcgcaa gcagcttttt cgcgccggtc
     1981 cggggcgttt tccagcgcca tttccacgtt gcgcagcaca gaaaagctga acgcgtaggg
     2041 ggactgcggc atgtagccca gcccgtccgg gttttggcgg ctcacttccc catgcgtggg
     2101 ttggagcgtg ccggccagca cgcgcaggag cgtggtcttg ccgctgccgt tctcgccaat
     2161 cagaccgtag cgcttgccct cctcaaaatc gagcaggggt atatccagaa tgacgcgccc
     2221 gccagcctgc accaaaacgt tccgcacgct gatcatgcca accccgcgct ttcctggatg
     2281 cggctgacca aagagttaat cagaaacgca attcccagca gcacaacccc cagcgcgata
     2341 gcgaactcaa agtgccccat attggtttgc atcataattg cggtggtcat cacgcgtgtt
     2401 ttgaactgca cattgccgcc caccatttgc accgcgccca cttctgcgat agcgcggcca
     2461 aaaccggtga gcacggtgga gatgaactgc acccgacatt catagatgat gtagagcatg
     2521 cgctgaaacg gccgcagccc gatgccggcg gcggtctcgt gtatcaaggg ggctttgacc
     2581 gagacaatat tcgcggtcaa gccggtcatg atgggcgtga tgagcacaat ctgggcgatg
     2641 accatggccg tgacggtgaa gagcagtttg agcgtaccca gggggccgct gcgggagaga
     2701 aacaggaaga caatcagccc ggccacgacc ggcggaaggc ccatcagcgt gtgcaaaacg
     2761 cggttgatga agcgtttggc tttgaaatcc gtactgccaa tgatgacccc gagcggaata
     2821 cccaacagcg ccgagataag cgtgctgagc gtggacattc gcagagtgac gccgataatc
     2881 tggcgcagct ccgggtcctg accgaatagc agtccgaaaa tctctctgat gacatccatt
     2941 tgtttgcggg cgcctgcggc ctttgcatag cagaaaaggc tggcaggcgg ggggtgccga
     3001 ggcgcggcac gggggagagc cgcgcctcag aacgcttaat agcggcttat tttgtggcca
     3061 ggatgaagaa cagctgctcg ccgtattctt cgataccgta cttcgcgatc aaatcggcgg
     3121 ctttttcaga ggtcatccat tcaatgaaag cctgggcgcc ggcgtaattg gtatccgcga
     3181 agcgctgggg ggagatggca atcagggaat aggtattctt catgtcgtcg gtttcttcca
     3241 gcagcagact gagggtgtta tcgtgcgcca gatacgtggc tttgtcggta aagacgtagc
     3301 cctgcatctc gttgccgacc gtcagggtct tgcccatgct gtcgcccacg ctgacgtacc
     3361 agggctgacc ggtggggtca agctcgaggg acttccagat tttcagctcg gctttatggg
     3421 tgccgctgtc gtcgccgcgg gagacgaagg tggcgccggc atcagcgatt ttcttgaacg
     3481 cttctgccgc agtagtggag cctttgacgc cggccgggtc gccttccgga ccgacgataa
     3541 cgaagaagtt atacatgaag ggcacgcgga cttcgccgaa accctcattg ataaattctt
     3601 cctcggaagc tttggcgtgc acaaggagta catcggcatc gccggtgcgc cccttttcga
     3661 tggcggcgcc tgtaccggct gaagtgattt cgagcgtgta gcccgtatcc ttctcaaaaa
     3721 ccggctgcag ataagccatc agaccggaat cgttgaccga ggtggtcgtc gagagccgga
     3781 tgacaggatt ctcaattttg gtagcgcagg ctgtcaagct gagaatcaat acccccatga
     3841 ttaaaacaac gcacaacttc ttcatatcat tctcctttcc aaatgggagg cgctgccgtt
     3901 tccagcaaca cccttgagcc cggggcgctt gggattttcc ccggacttcc gctcagctac
     3961 ccatgtcatt gatttaggcg tactgagtcg aagaaccagc attattatac ccggcgcgcg
     4021 tgaagcgtgt gtgataaaga gtacaaaaag ggttttagga ttattactct actgaggtgg
     4081 cgtaggggtc ttttgagctt cccccggggg ctgctggcgt gccagaggca aagctggcga
     4141 gcgcggtgag actgatgaag atacaagaag cagttcgtca tcgcgagaag cgaagcggcg
     4201 cggcgatctg cccgttgaat tcctggctct caggcagact gccgcgcctc ctactttttg
     4261 ggtctgtctt catgtcagtc cggcagcggg ggctcgcagc gacggnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnncgcga gaagcgaagc ggcgcggcga tctgcccgtt
     4441 gaaattctgg ctctcaggca gactgccgcg ccccctactt tttgggtctg tcttcatatc
     4501 attccggcag cgggggctcg cagcgacggg aagatcacgc cctgcgggcg ccctccccct
     4561 ggagaatatt acacgagcct ttgccgaccg aacacaaaaa accacggcgc cccatcttgc
     4621 tttatccgcc tgaaggcggt attagcagca ggatgaaacg ccgcgaaatc gctcgtccag
     4681 aaaactcagg cgacgatgtt caccagccgg ccgggcacga taatcacctt gcgcaccggc
     4741 ttgccttcca gcgcggcgcg cacagcttcg cttgccagcg ctttctcctg cgcctccgcg
     4801 tcagtaatgc ctggtggaac caggatgcgg tctcgcagct tgccgttcac ctgcacgacc
     4861 agcgtgattt cctcgtcgcg cgcggcagct tcgtccacca caggccagct ttgcgtgtgg
     4921 atggagtagg gcttgcccag gcgctcccat agctcctcgg cgatgtgcgg gcagagcggc
     4981 gccagcatgc gcaggtagat atccgcgcac tcattccatt ccgggctgcc ccaggcgccg
     5041 gcgtttttgg cgcgccccat ttcgttcagc agctccatca agctggagac aatcgtgttg
     5101 aattcgaagt tctcgtagtc atacgtgata gcgcgcagca cctggtgggt cttgcgccgc
     5161 agcgcgcgca gcgtttccgg gctggcatcc gtctgtgcgg gctccagcag catcgcccac
     5221 acgcgccgca gccagcgcga attgccttcg ataccgctgc tgttccaagg tccgccaact
     5281 tcccagcgcg cgaagaaggt caggtacgag cgcacagtat ctgcgccgta gcgcgagacc
     5341 aggtcgtccg gagcgatgac gttgccgcgg gatttggaca tcttttcgcc gtcttcgccc
     5401 agcaccatgc cctggttgcg cagctgcagc atcggctcgg gaccgatggt gatgccgcaa
     5461 tcgcggcagg ctttatggaa gaagcgcaca tacatcaggt gcatggtggc gtgttcgctg
     5521 ccgccggtgt aggtatccac tggcatccac ttgcggtaga gcgccgggtc aaagggatag
     5581 gtgtcgtaat ccgggctgag gtagcgcatg aaataccagc tggagcacat gaaggtgtcc
     5641 atggtgtcgg tttcgcgctc agccgggccg ccgcagaccg ggcaggtggt gaagcgccag
     5701 gtggggtgca gcttcagggg gctctcgccg gtgggcagcc attccacgtc atccggcagg
     5761 agcacgggca gctgctcttc cgggacgggc agggttccgc agtgcgggca gtagataatc
     5821 gggatgggcg cgccccaata gcgctggcgg gaaatcagcc agtcgtgcag gcggtaggtg
     5881 accgcgccct tgccaacgcc cagtctctcg atataggcaa tggccgcttc catcgttttt
     5941 tcctccgggg tgccgctgag cgggccggaa tggaccatcg cgccgtgcgc ttccagcgcg
     6001 gcggtcattt cggcggggtc ctgctcgggg ctgccttcgg gctggatgac cacgcggatg
     6061 ggcaggccgt attggcgcgc gaattcaaag tcgcgctggt cgtgggcggg cacggccatg
     6121 atggcgccgc tgccgtagcc catcagaacg taatcggcgg tccagaccgg cacgcgttcc
     6181 ccactgaccg ggttgagcgc gtacgcgccg gtgaagacgc cagttttggt gcggtcgacg
     6241 gcctcgcgct gaatatcgga ttggcgtttg gcttgttcct gataagcgcg cacaatttct
     6301 gcctgttcgg cagtggtgag tttctccacc agggggtgtt ccggcgccag caccataaag
     6361 gtcacgcccc acaaagtgtc gggtcgggtg gtgaagacct caatcgggtc gccttgttcg
     6421 gaggtaaagg ttacaaaggc gcctttggat ttgccaatcc acttggtctg gagagttttg
     6481 atggtatccg gccaatcgat gtcggagaag tccagcagct cctcggcgta attggtgatg
     6541 cggaagagcc attggcgcag gtctttgcgg atgacgggcg tgccgcagcg ttcgcagtga
     6601 cggtcttcgc cctgcacctg ctcgcgcgcg agggtggtgt tgcagttggg gcagaagtcc
     6661 accggcgctt ctttttggta ggccagattg tgcttgaaaa actgcaggaa gaaccactgc
     6721 gtccagcggt aatatttggg gtcggagctg atgatttcgc tgttccagtc gaacatattc
     6781 cccatggaac gcagctgacc gcgcatgcgc gcgatgttgg cgtaggtcca ggttttcgga
     6841 tggatgcggc gcttgatggc ggcgttttca gcgggcaggc cgaacgcgtc gaagccgatg
     6901 gggaagaaaa cgttgtagcc ctgcatccgt ttgaagcgtg cgcgcgcgtc ggagggcgtc
     6961 atggcatacc agtggccgat gtgcaggtcc cctgaagggt aagggagcat agtcagggcg
     7021 tagaatttct gcttttccgg gtggtcggcg gtttcgtaaa gtttatccgc tgcccagcgg
     7081 gcctgccatt tgggctcgat ctcctcaggt ttgtaaggcg gaatgctggt tgggtcaatc
     7141 atgctttagc tcctttaaat aaaaaatccc tgcgtgcagg gacgaagttt ccttcgcggt
     7201 accaccctgc ttcccggggt atccgggcag cttgggtttg cgataacggg caagaccgtc
     7261 tgcagctact catttcaccg cggccacccc tcggttgagg aaacaggcga gttgagcctt
     7321 gggcgctccg cgggcgcgtg ccggaattgc agtcttgtct ccggctctct ggcggacggc
     7381 ttagtactcc tgttatggtg tttcgagggt gattttatca caaaagagag aagtatagga
     7441 aaatttgacg ggagtgtaaa gcatttgaga aatgggagta taagaaaaga tagataatgg
     7501 tggggctaaa tgaaaagcat tacaaatcaa ttatgccaaa atattcatat cataaggatg
     7561 aatttcatct ataagaatcg cttttattag gttagaccga gaatccagcc aaacgtcggc
     7621 ggtttttgtg atttagaaat gatatgatga gtactactgc cttgttttat gttgctgtat
     7681 gaggtgaaaa atgacagaag aaccaaaaaa tgacgatgaa atgacnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnngttgc tgtatgaggt gaaaaatgac agaagaacca
     7861 aaaaatgacg atgaaatgac acaaaaaata tatcattcct ccatgcatca tgcaaacatg
     7921 gaacacgaac atcatatgca tgaaatgacc gggatggacc atgaccacag taagatgcct
     7981 gccagtcatg acgctcatgc cggtcatgaa atgaatatgc acgagatgga catgggaggg
     8041 catggagatc ataacatgcc catggaacat ggtggacatg aagaccacag cgcccacgtg
     8101 gatcattccg ggcatgagca gatgttccga cgaaaatttt gggtttcgct gatactttct
     8161 ctgccggttc tgcttttcag ccagggtttc caggccttgt tgggattttc aatgcctgtg
     8221 ttcccatgtt cgcgctggat cacgcccatc tttgcagtga tcatctttgt ttatggtggg
     8281 ttgcccttca ttaagatggc agtccctgaa ttacgcaacc gccagccggg catgatgacc
     8341 ttgatttccc tggccatcat cgtcgcattt gtttatagtg tggccagcct gttcctggcg
     8401 aaccaaatgg atttcttttg ggagcttgcg acactgatcg tcataatgct tctgggacat
     8461 tggatcgaga tgcgttccat ccgccaggca tccggcgcac tgaacgccct ggccaagctg
     8521 atgccagact ctgccgagcg cattgatgcg aatggtcaaa caacgaaagt ggtcgtgtct
     8581 gatctaaaat ccggagaccg gttgcttgtt cgtccgggat ccagtattcc ggcagatggc
     8641 gtagtggagg atggcgagtc agcagtcaac gaatccatga tcactggcga atcgaaaccg
     8701 gttcacaagc acccaggtga aaaggttgtg ggaggttcga tcaatggaga cggaagtttg
     8761 cgtatacgtg tgacggcaac cggtgaccag acggcgcttg ccggaatcat gcgcctggta
     8821 cagcaggctc agatgtcaaa aagccgcact caactgttgg ctgaccgagc tgccgggtgg
     8881 ctgttctaca ttgcactggg caccgccgtg atcactgcca tcgcctggac gattggggtc
     8941 ggcttcaacg taacggtgat cgaacgtgtg gctaccgtgc tggtgatcgc ctgcccgcat
     9001 gcgctgggtc ttgccatccc gctggtggta gccatcacca cagcaatggg tgccaacaat
     9061 ggcatcctgg tgcgcaatag attggcgctt gaagcggcgc gtgagatcaa caccgttatc
     9121 tttgacaaaa ccggaacact gacagaaggt cgttttgggg tggtggggat cgaaacactg
     9181 gatgggatca aaccggagga tgctctggcg ttgacagcag ccgtggaagg tgattctgag
     9241 cataccatcg cccagggcat ccgccgctct gcgcagcaga agggtcttca actgcctgta
     9301 gtacaggatt ttgaagcgat caaaggccgt ggtatccacg ccaaaatgag tgggcatgat
     9361 gtctacgtcg gcggaccgcg actactggaa atgctgacca tcacgctgcc cgaatcccta
     9421 tccatttttg accaggccgc aagcgccaaa gggcagagtg tggtccatct tgtacaggat
     9481 ggcaagccgc tggcgtccct ggcattggct gacgtggtcc gtccggagag ccatgaagcc
     9541 atccgtcggc tgcatgaaat gggcgtgcaa gtggccatac tgaccggtga cagccaggca
     9601 gtggccaagg cggtggctgg tgaactgggt atcgatacct attttgctga ggtgctgccg
     9661 gaaaacaaag accagaaggt gatcgaactg cagaagcagg gacggcgagt ggcgatggtg
     9721 ggcgacggtg tgaatgatgc tccagctttg acgcgtgcgg atgtgggcat tgccatcggc
     9781 agcggcacgg atgtggccgt ggagagcgcc gggatcatcc tggtgaaatc caacccgttg
     9841 gatgtggtca aggtgatcga actgagccgg gccagctacc gcaagatgcg ccagaatctg
     9901 atctgggcgg caggttacaa cgtggtcgcc attccactgg cagccggagt gctagccccc
     9961 tggggtatct tactttcacc cgctgttggc gccatcttga tgtcactgag cacggtcatc
    10021 gtggccatca atgcccagtt gctgcgcact aagcatttat gattagcgta aagatggcgt
    10081 tttgggaatt aaccccataa atccgctgat gtacctatgc ccttgataat ttccgtaagg
    10141 tagttatcaa gggcataaaa taaatcgcat gtcggtatta aacagcctaa aatctttctg
    10201 aaggttggga ttaagtgatt ggataaataa ataaattctt cactaatttt gggtatcgac
    10261 gctatttatg atggtcgcgc actctgatac ttgattcaaa gtacaatgat taaaaaagtt
    10321 accaggcttt tggacaagta tcagttgtca tcatcataaa ccatctactt tcctaaactt
    10381 acatccgaag gctgcctgtt tccccaccat ggtccaggat aatctttcaa cggtattcca
    10441 ccagattcca ggcacttcat accacttagc atgtgccaca tcctttggaa tatctggatc
    10501 tggtgcggta cctcgcgcaa gagctccatc cggatgaatg ccaacttcag tgtaaacaat
    10561 tgaataaatt gggcctaaag tggctaaaga caacatcatc ccccacccgc accgggaagg
    10621 ttctactgtt tttgcggcat ctatccggtc agcaacgaat tcgaagtagg tcatcccttt
    10681 tgg
//
LOCUS       DAOQAD010000063        10644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_7178_length_10644_cov_6.016527, whole genome shotgun sequence.
ACCESSION   DAOQAD010000063 DAOQAD010000000
VERSION     DAOQAD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10644
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7178_length_10644_cov_6.016527"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKL60_06075"
     CDS             complement(<1..530)
                     /locus_tag="PKL60_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNW95736.1"
                     /translation="MTKNKIKIILVLLAVFVFSSACMLTNAAREAVSQLQQSPTATAT
                     QQEQATALPPAGSTTIIEPEFANLEGTLEGLYQQVNPGVVSIRVSSVNDEALGSGFVI
                     DKQGHIVTNYHVVENADTVEVDFPSGLKVYGEVIGTDLDSDLAVIKVEVPEEELTPLT
                     LGDSDTALVGQTVVAIG"
     gene            complement(709..2637)
                     /locus_tag="PKL60_06080"
     CDS             complement(709..2637)
                     /locus_tag="PKL60_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNW95737.1"
                     /translation="MTSFKQQPGLRRFIPTSLKLLQPVPADIDIAQAAVLKPIAQVAE
                     EAGLLPEEIELYGDYKAKIKLDVLERLKDIPDGKYIDVTAITPTPLGEGKTTTTVGLS
                     QALGAHLGQRVFTCIRQPSQGPTFGIKGGAAGGGYSQVIPMEDFNLHLTGDIHAITAA
                     NNLLAAAIDTRMFHESSQSDRALFDRLCPMDKHGKRHFSVGMQARLRKLGITETDPEK
                     LTNEERARFARLDIDPATVTWRRGVDVNDRMLRGITIGQGPEEKGFELHTNYYITVAS
                     ELMAILALSTSLGDMRARIGRIVIGQSRSQEPITADDLGVAGALTVLMKDTIKPNLMQ
                     TLEGTPVLVHAGPFANIAHGNSSIIADMVALKLADYVVTESGFGADMGMEKFFDIKCR
                     YSGSIPNAVVLVATVRALKMHGGGPKVTAGTPLAAEYTNENLDLVRAGADNLLAHCRI
                     AAKFGIPVVVAINRFPSDTDAELELVRQIAVREEGVVDALVCDHYSRGGAGAVELGKA
                     VMKAANLPSRFEFLYPLEASIKDKIETIAREVYGADGVDYSEQAEARVKEYTRLGYDK
                     LPICMAKTHLSLSHDPALKGVPKGFRIPIRDISASIGAGFLYPLLGTMSTMPGLPTRP
                     AYYDIDIDFETGKILGLS"
     gene            complement(2651..4027)
                     /locus_tag="PKL60_06085"
     CDS             complement(2651..4027)
                     /locus_tag="PKL60_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95738.1"
                     /translation="MPARVRAALSLLLVLVLAAACAPAASPTPAPLSSTPSLPPNTPQ
                     PGEPTWTPSPVPFNPTATINFEAPNPVQISELQHVQLENHLVAAAKFQNTLEDAALRD
                     VQYEVLVLDANGSRLAQEFGAIPWLLPRQTSALVREFDLLAGAQAASVELRFTGGAPD
                     PKLKLRQPFTLADPAFIVDEASAQLTGWLSNADELTYTEVSINAIAYNAKGEIVGGGS
                     GFMEFVPPEDTIGVSVPATVTEAPTRVEIYPSLSAYSASLPGGRWWENIKVEDWSFVT
                     TSSGELAGGAVLTNITDRVLTGTFYILTVSDEQGRVCLVAKDFINMILPGETLNFSPG
                     VLRFPKTVSPSKVDLLIVPGEFGEYALAYNPLSTSQALLDTQGESPLVRVNVLNNLNA
                     GISTVLVNVLLKDPRGKIVGGGQILTEHVPPNGSLRIAVPVAFLGDPETLTVTTSATL
                     PVSAIIGQ"
     gene            4410..4583
                     /locus_tag="PKL60_06090"
     CDS             4410..4583
                     /locus_tag="PKL60_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95739.1"
                     /translation="MKILYIAGRWDPQFQTEYSGNDFGAYQALRSEPGIQLALAGPFD
                     YPPGWLERGLEKI"
     gene            4620..5540
                     /locus_tag="PKL60_06095"
     CDS             4620..5540
                     /locus_tag="PKL60_06095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNW95740.1"
                     /translation="MSYPDKCAREIEKKIAETKPDLIFSRYSAPLAKLQLGTPLVYMC
                     DSIVPFTRELAAEFSRPAYRVMEQWERAVIGKARRVITYSQANADLIVSEYGKAAEKI
                     TVLPIPAHVPRELRPDPSDDHKALDTPLRLLFVGKRAYLRGVDRALETARQLNAEGIP
                     AELRVVGMNGADEEHIRYMGVYDKEDPAALRAYFANFAWAQLLLHPSRFHAAGIVISE
                     AAAFGVPALTNAVGGLATSVLDGQTGLVLPAGSPPADYCRAIKELMREPLRYQEFRAN
                     ARRRFEAELDWEKAGQRFVGIIRAAAGEGE"
     gene            complement(5712..6041)
                     /locus_tag="PKL60_06100"
     CDS             complement(5712..6041)
                     /locus_tag="PKL60_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95741.1"
                     /translation="MIVKAWNNGERSSDGNGYGVKLSVRDRDRYFIPSWKTVSIELEG
                     NPDSIAVNVDKPSFWNKTCRELIHKEIGKWLIKNGLSDWPPYHPPALELQVINERKFF
                     LKLPTSK"
     gene            complement(6269..6352)
                     /locus_tag="PKL60_06105"
     tRNA            complement(6269..6352)
                     /locus_tag="PKL60_06105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6316..6318),aa:Leu,seq:taa)
     gene            6325..7989
                     /locus_tag="PKL60_06110"
     CDS             6325..7989
                     /locus_tag="PKL60_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HNW95742.1"
                     /translation="MRLPIPPPPRLQLPYFTIIAAPQARLRYNSGMQLSPLAWFLSFL
                     PVLVVIALMLGLRLSGARASLAAWLSAVLISLLAFGGTWQLLAWAQLKALWMALDVLY
                     IVWGALLLYHLVQEAGAIQALSARLPAISADRGLQVLLVSWLMVSFLQGMGGFGVPMA
                     VCAPILVGMGYPPVTAVVMTALGHAWAVNFGSMSTAFTSLLVVTGLPGALLAPFSALL
                     LGLAILPAGALVTLVGTGWKGLRRSFPALLVISLVMASGQYLLAVNGLWPLAATGSAV
                     LGILTLLLLTRLPRYRKAEAPAPLPANKPAGQDGFWWRVSPYLLLMLIGFAVVLIKPL
                     NAFLSQANLQPKFPELSTALGWVSPAEVGRPFHFFSHPGSILLYTCLISAGIYASKGL
                     LSPGAPARILGAVRASAGDVTLGVIAMVGVAAVMTASGMTNLLARGLSQSIPRAVYPA
                     VAPFIGALGAFLTGSNNNSNVLFGVLQMDTARLLGLNVPLILAAQTAGGSLGSVLSPA
                     KVIVGCSTVGLTNQEALVMRKLVPMELIPVGVVALAALLISALPGG"
     gene            8205..9788
                     /locus_tag="PKL60_06115"
     CDS             8205..9788
                     /locus_tag="PKL60_06115"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNW95743.1"
                     /translation="MSAARGRNLQTLFKRMPAFAASTLPDVRIRGVSMDSRTVRPGWM
                     FAAIQGENANGYDYIPQALANGAAAVMGTLPAPAGLNAPYLQIAGDIHQAVAYLAAAL
                     HDFPARKLRMLGVTGTDGKTTTTSMVYHILRSAGIRAGMISTVSAQIGEHVLDTGFHV
                     TTPEAPDIQGYLAEMVRAGLSHCVLETTSHGLAQGRVAACDFDAAVITNVTHEHLDYH
                     GSYENYLRAKGMLFESLSQTPRKRSGNLRAAVLNKDDQSYAYLKRVSPARQVSYSMLN
                     EADLWADEIDNQPTELAFTCHFGEGKALRVRTPMTGLYNVSNSLAALGLCVNLLGVPA
                     ETAVQALASLPGVPGRMERIDLGQPFNAIVDFAHTPNALSRALETARGFTQGRLIAVF
                     GSAGLRDREKRKMMPAVSIQQADLTYLTAEDPRTESLDAILQDMADSAVEAGGREGET
                     FWRVPDRGEAIRAALQAARPGDLVIVCGKGHEQSMCFGKTEYAWDDRQAVRSALAELL
                     GVPGPAMPWLPTSKERPGA"
     gene            complement(9867..10316)
                     /locus_tag="PKL60_06120"
     CDS             complement(9867..10316)
                     /locus_tag="PKL60_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95744.1"
                     /translation="MNNYKRPSPFDPRGTKIAFLVLSALMNIAAGLAFFSLVDWLMLT
                     YGDLVSGIDTTLMLGMFLISLMIGFVMSQIAADSRAVTYGVYGGLAGMVLSALRIWNS
                     SLVLAALVGLVCVLGGYNGGMLGEGVRRMRAKQRKKIQRGEGNARLK"
     gene            complement(10388..>10644)
                     /locus_tag="PKL60_06125"
     CDS             complement(10388..>10644)
                     /locus_tag="PKL60_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95745.1"
                     /translation="HARGPVCAENLIWREFLQRWGKILGKPKPILTLPKALESLGAWF
                     ITLTQRMLCREGAPELVPFVDLQTRLAFLDPDYSAREPGM"
BASE COUNT         1981 a         3280 c         3189 g         2194 t
ORIGIN      
        1 ccaatcgcga ccaccgtctg accaaccaat gcggtgtcgg aatcgcccaa agtcagaggg
       61 gtcagttctt cttctggtac ctccacctta atgaccgcca ggtcagagtc caaatcggtc
      121 ccaatgacct cgccgtatac tttcagaccg gatggaaaat caacttccac tgtatccgcg
      181 ttctcgacca cgtggtagtt ggtgacaata tgcccctgct tgtcaatcac gaaaccggag
      241 cccagtgcct catcgttgac cgagctgaca cggatggaca ccacgcccgg attgacttgc
      301 tggtacagtc cttccagcgt gccctccagg ttggcaaact caggttcaat aatggtcgtg
      361 ctgccagcgg gaggcagcgc cgtggcttgt tcctgctgtg ttgcggttgc tgtcggggac
      421 tgctggagtt gcgataccgc ctcccgggcg gcattggtga gcatacaggc agagctgaaa
      481 acaaacactg ccagaagcac gaggatgatt tttatcttgt ttttagtcat ttagcacctg
      541 caattctggt tatgataaga aaccagtttt attgtagcga ttttcaccgg ccggcgtcaa
      601 gccgcagcgt tctattttta tcaaattcat agcaagcctg aagacctttt tcaggaaaac
      661 aaaacgccgg caggcaaatc cagccggcgt tccgtggtaa gcgggagttt aactcaagcc
      721 aaggattttg ccggtttcga aatcaatgtc aatatcatag tacgccggcc gggtgggcaa
      781 gccgggcatc gtgctcatcg ttcccagcag cgggtaaagg aagcccgccc caatggacgc
      841 gctgatgtcc cggataggga tgcggaagcc cttgggcacg cccttgagag cggggtcgtg
      901 gctgagcgag aggtgggtct tggccatgca gatgggcagc ttgtcgtagc ccaggcgggt
      961 atattctttc acgcgcgcct cggcttgctc gctgtaatcc accccatccg cgccgtacac
     1021 ctcgcgcgcg atggtctcaa tcttgtcttt aatcgaagct tccagtggat aaaggaattc
     1081 gaatctggaa ggcaaattgg cggctttcat cacggctttg cccaattcca ctgccccggc
     1141 cccgccccgg gaataatggt cgcataccag cgcgtccacc acaccttctt cccgtacggc
     1201 aatctggcgc accagttcca gctcggcatc ggtatccgag gggaagcggt tgatggcgac
     1261 gaccaccggg atgccaaact tggcggcgat gcggcagtgg gccagcagat tatccgcgcc
     1321 ggcgcgcacc aaatcaaggt tctcattggt gtattccgcg gccaacggcg tgccggcggt
     1381 cactttgggg ccgccgccgt gcatcttgag cgcccgcact gtcgccacca gcaccactgc
     1441 gttcgggatg ctgccggagt agcggcattt gatatcaaaa aacttctcca tgcccatgtc
     1501 cgcgccaaag ccggattcgg tgaccacgta gtccgccaat ttcagggcga ccatatcggc
     1561 aatgatggag ctgttcccgt gcgctatgtt ggcgaaaggc ccggcatgca ccagcacagg
     1621 cgtgccttcc agcgtctgca tcaggttagg cttgatggtg tctttcatca gcacggtcag
     1681 cgcgccggcc acccccaggt cgtccgcggt gataggctcc tggctgcggc tctgcccgat
     1741 gacgattctg ccgatgcgcg cgcgcatatc ccccagactg gtggaaagcg ccaaaatcgc
     1801 catcagttcg ctcgcgacgg taatatagta gttggtgtgc agctcaaaac ctttttcttc
     1861 cggcccctgc ccaatcgtaa tcccgcgcag catgcggtcg ttcacgtcca cgccgcgccg
     1921 ccaggtgacc gtggcggggt caatgtccag ccgcgcgaag cgcgcccgtt cctcattggt
     1981 cagcttttcg gggtcagttt ctgtaatccc cagcttccgc aggcgggctt gcatcccgac
     2041 cgaaaaatgg cgcttcccgt gcttatccat cgggcacagg cggtcaaaca gcgcccggtc
     2101 gctctggctg gactcgtgga acatgcgcgt gtcgattgcc gcggcgagca ggttgttggc
     2161 cgccgtaatg gcgtggatat cgccggtgag gtgcaggttg aaatcttcca tcgggatgac
     2221 ctgtgaatac ccgccgccgg cagccccgcc cttgatgcca aaggtgggac cctgtgatgg
     2281 ctgccggatg caggtgaaca cgcgctgccc caggtgcgcg cccagcgcct ggctgaggcc
     2341 tacagtcgtg gtggttttgc cctcgcccag gggcgtcggg gtgatggcgg ttacatcgat
     2401 gtattttccg tccggtatgt ccttcaggcg ctccagcacg tccagtttta ttttggcctt
     2461 gtaatcccca tacagctcga tttcttcggg cagcaggccg gcttcttcag ccacctgcgc
     2521 gatgggcttc agcaccgccg cctgcgcgat gtcaatatcc gcggggacag gctgcagcag
     2581 cttcaggctc gtgggaataa aacggcgcaa tccaggttgt tgtttgaatg aagtcacttt
     2641 gctcctttgt ttactgcccg atgattgccg acaccggcaa ggtggcagat gtggttacag
     2701 tcagggtttc ggggtccccc aggaaggcca cgggcacggc tatgcgcagc gagccgttgg
     2761 gcggcacgtg ttcggtcagg atttgcccgc cgccgacgat tttccctcgc gggtctttta
     2821 gcagcacatt caccagcact gtcgaaatcc cggcgttcag gttattcaac acgttcacgc
     2881 gcacgagcgg gctttcgcct tgcgtgtcca acagcgcctg cgaggtggaa agcggattat
     2941 acgccagcgc gtactctcca aattcccccg gcacgatcag caggtccacc ttgctcgggg
     3001 aaacggtctt cgggaagcgc agcaccccag gtgagaagtt tagcgtttct cccggcagaa
     3061 tcatgttgat aaagtccttc gccaccaggc aaacccgtcc ttgctcgtcg gaaactgtca
     3121 gaatgtagaa cgtgccggtc aatacgcggt cggtaatatt cgtcagcacg gcgccgccgg
     3181 ccagctctcc ggaactcgtg gtcacgaagc tccagtcctc caccttgatg ttttcccacc
     3241 acctgccccc cggcagcgag gcggaatagg cggaaagcga ggggtaaatc tccacgcgtg
     3301 tgggggcttc cgtcacggtg gcaggcacgc tcaccccgat agtgtcttcg ggcggaacga
     3361 actccataaa tccgctcccg ccgcccacaa tctcgccttt ggcattatag gcgatggcgt
     3421 tgatggagac ttccgtgtag gtcagctcgt ccgcgttgct cagccagccg gtcagctgcg
     3481 cggaagcttc gtccacgatg aaagccgggt cggcgagcgt gaaaggctgc cgcagcttca
     3541 gctttgggtc cggggcgccg ccagtaaagc gcagctccac ggaggcagct tgggcgcctg
     3601 ccagcaagtc aaattcgcgc acgagagcgc tggtctggcg cggcagaagc caggggatcg
     3661 ccccaaactc ctgcgccagg cggctgccgt tggcatccag cacgagcact tcgtactgaa
     3721 catcccggag ggcagcgtct tccagcgtgt tctggaactt ggcagccgcg accaggtggt
     3781 tctccaactg cacgtgctgc agctcgctga tttgcaccgg attgggcgcc tcaaagttaa
     3841 tcgtcgcggt cgggttgaag ggcacgggcg aaggcgtcca ggtcggttcg ccgggttggg
     3901 gcgtgttggg cggcaggctg ggcgtggagc tgaggggtgc gggcgtgggg ctggctgcgg
     3961 gcgcgcaggc ggcagccagc accaggacga gcagcagtga gagcgcggcg cggacacggg
     4021 cgggcatcag atggacctcc cggatgccca aaaagacaac gcgaacaatg ctcctccgaa
     4081 aattccagag gtcgttgcag agattaaatg caggctgggt gttttcatcg ttgcttggct
     4141 ttcccgtgag caaggcgcgc gcaaacaagc gccgaacctg ctgcaatttt actattgttt
     4201 tccagttccc ggatttccgt atcggcagat aagaccggta ctgccgggaa aactgccgac
     4261 acctgggatt agcgtatggc tatggatttg ctgcatgaaa acggggacta ttggcaagct
     4321 gtgctaaaat gcagtggaaa cttttcgaag tgacaggatt aggattctga agaccacgca
     4381 tgaaggctta aaatggagag ggtgggtaaa tgaaaattct atatatcgcc ggacgatggg
     4441 atccgcaatt tcagaccgag tacagcggca acgattttgg cgcgtaccag gccttgcgca
     4501 gcgaacccgg catccagctt gccctggcag ggccgttcga ctatcctccc ggctggcttg
     4561 aacgcggcct ggaaaaaatc taacggaagc tgaccggcaa gcgcctgctc aagttcccct
     4621 tgtcctaccc tgacaaatgc gcgcgggaaa tcgagaagaa aatcgcggaa acgaaaccgg
     4681 acctgatctt ctcgcgctac agcgccccgc tggcaaagct gcagctgggc acgccgctgg
     4741 tgtacatgtg cgattccatc gtccccttca cccgggagct ggccgccgag ttctccaggc
     4801 ccgcctaccg cgtcatggag cagtgggagc gcgccgtcat cgggaaagcc cggcgcgtca
     4861 tcacctacag ccaggccaac gccgacctga tcgtctccga gtacggcaag gcggccgaga
     4921 agattacggt cctgcccatc cccgcgcacg tgcccaggga actgcgcccg gacccatccg
     4981 atgaccataa agcgctggac acgccgctgc gcctgctctt cgtcgggaag cgcgcctacc
     5041 tgaggggcgt ggaccgcgcc ctggagaccg cgcgccagct gaacgcggag ggcatccccg
     5101 cggagctgcg cgtggtgggg atgaacggcg cggacgagga gcacatccgg tatatgggcg
     5161 tgtacgacaa agaagacccc gcggctttac gggcgtactt tgccaacttt gcctgggcgc
     5221 agctgctttt gcacccttcc cgcttccacg cggccgggat tgtcatcagc gaggcggcgg
     5281 cttttggcgt gccggcgctt accaacgcgg tcggcgggct ggccacttcg gttttggatg
     5341 ggcagaccgg gctggtgctg ccggcgggca gccccccagc ggattactgc cgggcgatca
     5401 aggagctgat gcgggagcct ttgaggtatc aggaatttcg ggcaaacgcg cgccggcgtt
     5461 ttgaggccga gctggattgg gagaaggcgg ggcagcgctt cgtcgggatt atccgcgcgg
     5521 cggctggtga gggggagtaa ggaaagagtg actggggtga attcgagctc tctaatcacc
     5581 gaatcaacga cgccactgca atacactcaa gctccataca ctggctattt cagacaatta
     5641 gtttctgaca aaattgggca actttaacgt cgaccaaacc gtagggtact ttgacaacta
     5701 acttcactga cctatttgga ggtcggaagc tttaaaaaga attttcgctc attaattact
     5761 tgaagttcga gcgcaggagg atggtaaggg ggccaatcgc ttaatccatt tttgatgagc
     5821 catttaccaa tctccttgtg gattaattct cgacaggttt tattccagaa gcttggttta
     5881 tcgacgttga cggcaataga atccggattg ccttcaagct caattgagac ggttttccaa
     5941 ctcggaataa aatatctatc tctatcacga acagagagtt ttacaccata tccattacca
     6001 tcagagcttc gctctccatt gttccatgct tttactatca ttaaatcctc ctctaatagt
     6061 ccatgaagtt ttgcattctc ttggtgcttg tcagtctgct gaccgagtaa tattgttaga
     6121 gcaggttact aaattcattt gattgccgtc acttgctaac gccaggtatt taagcaatca
     6181 atacttacca caatccctta aacaccattt ttcccaccta aagactaaac aaaaaccgag
     6241 ccccagctcc ttctttcatc cccattcata gggcgggtgg gggtcgaacc cacacagtgt
     6301 ctccaccgac ggattttaag tccgttgcgt ctgcctattc cgccaccgcc ccgtcttcaa
     6361 ctcccctatt ttaccatcat tgccgccccg caagcccgcc tgcgctataa ttccggcatg
     6421 cagctctccc ccctcgcatg gttcctctcc ttcctgccgg tgctggtcgt catagccctc
     6481 atgctcggcc tgcgcctcag cggcgcgcgc gccagcctgg ccgcctggct cagcgccgtc
     6541 ctcatcagcc tgctggcttt cgggggaacc tggcagctgc tcgcctgggc gcagctaaag
     6601 gcgctctgga tggcgctgga cgtgctctat atcgtgtggg gcgcgctcct gctctaccac
     6661 ctggtacagg aggccggcgc catccaggcc ctcagcgcgc gcctgcccgc catttccgcc
     6721 gaccgcgggc tgcaggtgct gctggtctcc tggctgatgg tgtcctttct gcaggggatg
     6781 ggcggcttcg gcgtccccat ggcggtctgc gcgcccattc tggtcgggat gggctacccg
     6841 ccggtcaccg cggtggtgat gacggcgctc gggcacgcct gggcggtgaa cttcggctcg
     6901 atgtccaccg cgttcacgtc cctgctggtg gtcacgggct tgcccggagc gctgctcgcg
     6961 cctttttccg cgctgcttct gggcctggcc atcctgcccg ccggcgcgct ggtgacgctg
     7021 gttggcacgg gctggaaagg cttgcggcgc agcttccccg cgctgcttgt gatttccctg
     7081 gtgatggctt cagggcagta cctgctggcg gtcaacggct tgtggccgct ggcggccacc
     7141 ggctcggctg tgctcgggat cctcacgctg ctgctgctca cgcgtctgcc gcgctaccgc
     7201 aaggccgaag cgcccgcacc cctgcccgcc aacaagcctg ccgggcagga cggcttttgg
     7261 tggcgcgtct cgccttacct gctgctgatg ctgattggtt tcgcggtggt gctcatcaag
     7321 ccgctgaacg ctttcctgag ccaggccaac cttcagccaa aattcccaga gctcagcacc
     7381 gcgctgggtt gggtcagccc cgcggaagtg ggacggccgt ttcacttttt ttcgcacccg
     7441 ggctccatcc tgctctacac ctgcctgatt tccgccggca tctacgccag caaaggcttg
     7501 ctctcgcccg gcgcgcctgc ccggattttg ggggcggtac gcgcgagcgc cggggatgtc
     7561 acgctggggg tcatcgcgat ggtcggcgtg gctgcggtga tgaccgccag cggaatgacc
     7621 aacctgctgg cgcgcggact gagccagagc attccccgcg cggtctaccc ggccgtcgcc
     7681 ccgttcatcg gcgcgctggg cgccttcctg accgggtcga acaataattc caatgtgctc
     7741 tttggcgtgc tgcagatgga taccgcccgc ctgctgggct tgaacgtccc gttgattctc
     7801 gccgcgcaga cggccggcgg ctcgctgggc agcgtgctat cccccgcgaa ggtgattgtg
     7861 ggctgcagca cggtgggcct gaccaaccag gaagcgctgg tgatgcgcaa gctggtgcct
     7921 atggaactca tcccggtcgg ggtggtcgcg ctggcagcgc tgctgatttc ggcgctgccc
     7981 ggcggctgac gctgccgccc gcctttcgtt tcgaagctgc ggacatgccg cccgcttctt
     8041 tctcaaaact ccgcgcccgg ttcgcatgtt tatggcgcgc gtgccgcggc tgagcgcgtc
     8101 aggtccggag cgctttttga actgtaaaga aaaggcacga ccacaagttg gcaccggcgg
     8161 atgcaggttt tccggttttc gggtacactc ttcgcgaggc aacaatgagc gctgcacgcg
     8221 gtagaaatct ccagacgctt tttaagcgca tgcccgcctt cgcggcatcc actcttcctg
     8281 atgtccgcat tcgcggggtc agcatggatt cgcgcacagt ccgcccgggc tggatgttcg
     8341 ctgccatcca aggagagaat gccaacggtt acgactacat cccgcaggcg cttgcgaacg
     8401 gcgcggcggc ggtgatgggc acgctgcccg ctccggccgg cttgaacgcc ccctacctgc
     8461 aaatcgcggg tgatatccac caggcggttg cttatctggc cgccgccctg cacgattttc
     8521 cggcgcgcaa gctgcgtatg ctcggcgtga cgggcacgga cggcaaaacc accacgactt
     8581 ccatggttta tcacatcctg cgcagcgccg gcatccgcgc cggcatgatt tccacggtca
     8641 gcgcgcaaat cggcgagcac gttttggata ccggcttcca cgtcaccacg cccgaagcgc
     8701 ccgatatcca gggctacctg gccgagatgg tgcgcgccgg gctctcgcac tgcgttttgg
     8761 aaacgacctc ccacgggttg gcgcaggggc gcgtggccgc ctgcgatttt gacgccgctg
     8821 tgattaccaa cgtcacgcac gaacacctgg actaccacgg ctcgtacgag aactacctgc
     8881 gcgccaaggg catgctcttc gaaagccttt ctcaaacgcc gcgcaaacgc agcgggaacc
     8941 tgcgcgccgc cgtgttgaac aaagacgacc agtcctacgc ttacctcaaa cgcgtttcgc
     9001 cggcgcgcca ggtcagctac agcatgctga acgaagctga tttgtgggcg gatgaaatcg
     9061 ataaccagcc caccgagctg gccttcacct gccacttcgg ggagggaaag gcgctgcgcg
     9121 tacgcacgcc gatgaccggg ctctacaatg tgtccaacag tctggcggcg ctgggtttgt
     9181 gcgtgaactt gctgggggtg ccggcggaaa ctgccgtgca agccctggcg agcctgcccg
     9241 gagtgcccgg gcgcatggag cgcatcgacc tggggcagcc cttcaatgcg attgtagatt
     9301 tcgcgcacac ccccaacgcg ctctcgcgcg cgctggaaac cgcgcgcgga ttcacacaag
     9361 ggcggctgat tgcggtgttt ggctcggccg gcctgcgaga ccgcgaaaag cgcaaaatga
     9421 tgccggcagt ctccatccag caggccgacc tgacttacct gaccgcggaa gacccgcgta
     9481 cggaatcgct ggacgccatc ctgcaagaca tggccgactc ggctgtggaa gccggcgggc
     9541 gcgagggcga aacattttgg cgcgtgcccg accgcggcga ggctatccgt gccgcgctgc
     9601 aggctgctcg cccgggcgac ctggtgattg tgtgcggcaa agggcacgag cagtccatgt
     9661 gctttgggaa gaccgaatac gcctgggacg accggcaggc tgtgcgctct gcactggcgg
     9721 aactgttggg cgtgcccgga cccgcaatgc cctggctgcc aaccagcaaa gaaagacccg
     9781 gggcttagcc gggtttcaaa tcacgcgggg gggttttttt ccgttcattt tcagcgtttg
     9841 tcaggatcct gccgcgcatt ccccgctcat ttcagccgcg cgttcccctc gcctcgctgg
     9901 atttttttcc tctgcttcgc gcgcatgcgg cgcacgccct cgcccagcat gccgccgtta
     9961 tacccgccta aaacgcacac caagcccacc agcgccgcca gcaccaggga gctgttccag
    10021 atgcgcagcg ccgagagtac catcccggct aatcctccgt acacgccgta agtcaccgcc
    10081 ctgctgtccg ccgcgatttg gctcatgacg aagcctatca tcagcgagat taggaacatc
    10141 cccagcatca gggtggtatc gatgccgctt accaggtcgc cgtaagtgag catcagccag
    10201 tctaccaggc tgaagaatgc cagacccgcg gcgatgttca tgagcgcgga aagcaccagg
    10261 aaggcaattt tcgtgccgcg tggatcaaat ggagaagggc gtttgtagtt attcatcttt
    10321 tttcctgcag tcagaccgtg aagcccgcac catgttcaca aaaaaagcgg gttccaggtc
    10381 cgtgtgttca catccctggc tcccgggcgg aatagtccgg gtccagaaac gccaggcggg
    10441 tctgcaagtc cacaaaaggc acaagttccg gggcgccttc cctgcacagc atgcgctgcg
    10501 taagggtgat aaaccaggcg cctaagctct ccagcgcttt gggcagcgtg aggataggtt
    10561 tgggcttgcc cagaatcttg ccccaacgct gcaaaaattc gcgccagatc aggttctccg
    10621 cgcaaaccgg gccgcgagca tggg
//
LOCUS       DAOQAD010000064        10442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_7397_length_10442_cov_4.898720, whole genome shotgun sequence.
ACCESSION   DAOQAD010000064 DAOQAD010000000
VERSION     DAOQAD010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10442
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7397_length_10442_cov_4.898720"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PKL60_06130"
     CDS             complement(<1..529)
                     /locus_tag="PKL60_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95746.1"
                     /translation="MRRTSVLLVAIVLIASLVLGACAKATEAPVVTEAPTEAPVVTEA
                     PTEAPTEAPTEVDPWADVVPAKEIVWWHQHTGEAREAAIAKIVSDFNSTNEYGITVTA
                     EYAGSYGDIFNKMLPILNTPDVPDVVVGYQNQVATYQLANAMFDMNELINHPKYGLTK
                     EEQDDFFPGFFAQDVL"
     gene            complement(624..1496)
                     /locus_tag="PKL60_06135"
     CDS             complement(624..1496)
                     /locus_tag="PKL60_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNW95747.1"
                     /translation="MQKPKKEIKENHAGRILGKVLLYLILLIGAAIFIFPFFWMISNS
                     LMTLGETITRQLVPKVPQWVNYKEAWDQANFSKYFMNSVLVTLGTLAGLLFTSVLAGY
                     AFGRIDFKGKDILFGLFLATMMIPESVTLIPNYLLIRGTIIPLPGGTWINTYWALTVP
                     FMANAFSIFLLRQFFAQIPDELWDAARMDGCDHLRFLIQIVMPISKAPIMTVLLFGFT
                     ASWNAFQWPLLVTTRDTWRPLMVGLYGFVQEAGPYTHWLMAGAVITIIPILLVYFLTQ
                     KQFTQGIATTGLKG"
     gene            complement(1507..2667)
                     /locus_tag="PKL60_06140"
     CDS             complement(1507..2667)
                     /locus_tag="PKL60_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNW95748.1"
                     /translation="MAENTAVRQTRRKTLKLIKLQEQLTGYLFILPALIIILIFGIFP
                     IGYSIYMSLFNWQVTKGPFIGDKNYLLIFGSWWNLLVLLAGLGLIILGSIVWGKGFQS
                     LSNGQKLAHIAGSLVLILGGVIFVTGWGMMYETGNKDFLNSLIVTVYYALGTVPAEII
                     LGLVLAYILYQDIVGKEAFRIIYFLPYITPSISTAVVFQIIFSSRETSLANSLIGFFG
                     IDPLKWRFEPRPVLKLLGFNVSGLAGGPSLALVTIIIFGIWSFVGYNTVIFLSGLGNI
                     PKEIYEAAEIDGGSKWDRFWHITIPMISPVTFYLTLIGFIGTFKAFNHLYVMRTPSAQ
                     NTVITTSLLVFDRFYKANQYGVATAQAIVLFIVILGLTLAQNKIMGERVFYG"
     gene            complement(2786..3823)
                     /locus_tag="PKL60_06145"
     CDS             complement(2786..3823)
                     /locus_tag="PKL60_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95749.1"
                     /translation="MPGDVSPELSSKEDAPTAAAETTVPTETAAAEKPAEADVRVIKL
                     PAPYDDARMEFSGMAWLGDKLVLLPQYPRRLGEDGSGFIVTIQKEDILAYLGGESMEP
                     IETGLIAFDDGGLSESLEGFEGFEALAFLGEKVFLTIETSGGSPMMGYLVSGVVDEER
                     QSIRLEAEGIVPLVPQTDFRNASDEALVVANEQLFTFFEDNGSAVNPYSYAHVFDTAL
                     SPQPQTDFPAIEYRVTDATEAQADGSFWVMNYFYPGDAHLKAEVDPIAQRFGEGLSHR
                     RSDRVERILRLQLENGQIRMADEAPLYLSLTPDGSSRNWEGLVRLDELGFLAVTDSFP
                     ETILGFIPDLR"
     gene            complement(3925..5784)
                     /locus_tag="PKL60_06150"
     CDS             complement(3925..5784)
                     /locus_tag="PKL60_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNW95750.1"
                     /translation="MKKQRSSILSFLCLVFILSGCLRPNSLQAPGAPSADAAAAVDSR
                     LPISAVQGAQHRSPYDGKKLSGVEGIVTALTGSGFYLQDPHPDDDERTSEALFVSASS
                     FGNLKAGDLVRIVEGKVREYNPAGLGENSLTRTEIFQVTYTILSSGNSLPEPVVLGEG
                     GRRIPDSVIEDDVNGYASKGGEFDPARDGLDFYESLEGMRVQVNAALAVSSTSSYKEV
                     AVVADMGKNAGLLSPRSVLILRKDDANPERILLDDTFINMPAIRTGARFTEPIIGIID
                     YSFGNFKLQPTQKLRFEQGNNPPEAVTYELSEDELAFATYNVANFNPMVSPERLEALA
                     EQIVFILRSPDILALQEIQDDDGELDSAQTSAAGNINRLLQTVQQVGGPVYKALSIDP
                     LRNADGGATGGNIRVVLLYREDRGLSLAAAPAGDASSANQVLKKDGKPVLSLNPGRVS
                     PQAYAFRDSRKPLSAQFSFQGRDIFVIACHFNSKGEDGPLYGDQQPPQLVSERQRMEQ
                     AREINEFVAQIIGINPEAYVVVLGDLNDFPWSPPIMKLQDGALANLITLLPEQDRYTY
                     NHEGNGQVLDQILVSLPCLSLVSHFDNVHINSEALPDARVSDHDPVYAVLKVE"
     gene            5867..6562
                     /locus_tag="PKL60_06155"
     CDS             5867..6562
                     /locus_tag="PKL60_06155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNW95751.1"
                     /translation="MEIQIAIAKVDRYSSPEQGDQVEMVERPNGGASVILGEGKLNGQ
                     RSKFVARKAVHRVLSLLLNGIHDGAASRAVLSALKTEYHGEAEFSLNVLSCDLESETI
                     VLTKNNDLPVIMIRKSEGSYTSYEGRDLVDPQEPNVYQFPIENDFIIIMFSDGVATAG
                     WDCSQPLEWNSLMETVLDEQELTVQELADQILNQAVAQDLGQPHDDMSVVVMQVGRTT
                     GKPIRRMSLTLPI"
     gene            6578..7609
                     /locus_tag="PKL60_06160"
     CDS             6578..7609
                     /locus_tag="PKL60_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNW95752.1"
                     /translation="MREEVVLITGANGEIGHGLIRQISAMNCPIVAIDVNPLDKPLRS
                     MVAKWIVGDILDQILLGRLVVEHQIRTIYHLASILSTKAEYNPETAHRINVEGTLNLL
                     RLAVEQSQWQGNSVKFIYPSSIAAYGMDSLDCKARLGKVKEDQYTFPTTMYGCNKLYC
                     EHLGRYYSKYYRQLAADSASGPSVDFRCLRFPGLISAETIPTGGTSDYGPEMLHAAAK
                     GTPYNCFVRPDTRLPFMVMPDAIKSLITLENAGHEKLTRFVYNVTSFNPTAQEIFEIT
                     TRAFPGARIGFEVDQRRQKIVDSWPENVDDSAARQDWGWQPDYDQERAFEEYLIPAIR
                     NQYTELANP"
     gene            7665..7889
                     /locus_tag="PKL60_06165"
     CDS             7665..7889
                     /locus_tag="PKL60_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNW95753.1"
                     /translation="MPIYEYQCRQCGAHFELLRSYNQSDSPAECPHCSGSETRRELSR
                     VNAFSGGKSLTDSGHSCGGCSGGSCSTCGH"
     gene            7892..8752
                     /locus_tag="PKL60_06170"
     CDS             7892..8752
                     /locus_tag="PKL60_06170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW95754.1"
                     /translation="MKNLNGKLALITGGSSGIGLALASKLFASGMSVAILARGQQRLD
                     EALAIIEKSRLNPAQKLTAISANVADAAGIAQVLREFVAGYGLPDLVINCAGVAKSGY
                     AEATPLEDFRWMMDIDYHGTVNVVQTLLPEFIARGSGHIVTISSLAGVVGVFGYTAYG
                     GAKFAVRGYTDALRAEMKSRGIQVSCVFPPDTDTPQLSYENQFKPYETHVIAGSDKPL
                     SAEKVADSIVRGIQKDKYAIVPGLEAKVLFFLVTHLGGLVYPVLDLLVKSASKKKAAL
                     QVAQTEKRGR"
     gene            8762..>10442
                     /locus_tag="PKL60_06175"
     CDS             8762..>10442
                     /locus_tag="PKL60_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNW95755.1"
                     /translation="MEIDELLNLWKRDPEFIRNTTCWQVLPPTGAVYEALPNNLPEQL
                     RCILVNRGITRLFSHQAQSLAHLSAGEHVVVSTGTASGKTLCYDIPVIETLLQNPDAR
                     ALYLFPTKALAQDQFRLLNDLCASPGGNLKPLPAIYDGDTPSNHRAEIRRKAEILITN
                     PDMLHQGILPHHTIWRQFFGGLRFVVIDEMHTYRGVFGSHVANVIRRLKRVAAFYQAY
                     PQFILTSATIGNPEELAEKLIESPVALVDQDGSPHGRRHFLVYNPPLVNEQLGLRASS
                     LQTGISFTRKLLSNSIQTLVFARSRRSVEMILTYLYDAVPGETRARIRGYRSGYLKSE
                     RREIEAGFKDGSIKAVVATSALELGIDIGSLESVILVGYPGSIATTRQRAGRAGRRQQ
                     ASLAMFIASPEAMDQYLANHPEYLTEKSPENALIDPNNYAILLQHLQCAAFELPFLEN
                     DRFGSLPPELLDSFLKILTQSGVLHLQNGRYFWIADQFAAGSVSLRSSTPNVITLRAA
                     DGDTTHVIGEIDAASAKWLVHPEAIYLHEADSYEVKALDLENGSCLLEPVQS"
BASE COUNT         2425 a         2949 c         2805 g         2263 t
ORIGIN      
        1 agagcacgtc ctgggcaaag aaaccaggga agaagtcatc ctgttcttct ttggtcagac
       61 cgtacttggg gtgattgatc agttcgttca tatcgaacat ggcattcgcc agttgataag
      121 tcgcgacctg attctgatag ccaaccacca catcagggac atcgggggtg ttcaggatgg
      181 ggagcatctt gttgaagatg tcgccatagc tgccggcata ttcagcggtg acggtgatac
      241 cgtattcatt ggtactgttg aagtcggaaa caatcttggc aatcgcggct tcgcgggctt
      301 caccagtgtg ttgatgccac caaacaattt ccttggcggg aacaacgtcc gcccagggat
      361 caacttccgt cggagcttcg gtaggagctt cggtcggggc ttcggtcacg acgggagcct
      421 cggtcggggc ttcggtcacg acgggagctt ctgttgcttt ggcgcaagcg cccagcacca
      481 gtgaggcaat caggacgatc gccactaaca aaacactcgt tcttctcatt tttcaaaacc
      541 tccattttga acctgattaa tttgagcgtt acgatatcga aggttgagag aataccttcg
      601 gaatatccaa agttgattat atcctaacct tttaaacccg ttgtggcaat tccttgagta
      661 aattgtttct gagtcaggaa gtacacaagc aggatcggaa taatcgtaat caccgcccca
      721 gccatcaacc aatgggtata gggaccggct tcctgcacaa atccgtacaa gccaaccatg
      781 agcggtcgcc aggtatcccg cgtggtaacc agcagcggcc actggaatgc gttccacgaa
      841 gccgtgaaac caaagaggag cacggtcatg atgggcgcct tgctgattgg catcacgatt
      901 tggatgagaa aacgcagatg gtcgcagccg tccatgcggg ccgcgtccca caactcatcc
      961 ggaatctgcg cgaaaaattg cctcagcagg aaaatactga acgcgtttgc catgaacggc
     1021 actgtgagcg cccagtaggt gttaatccag gtgccgcccg gtaaagggat aatcgtgccg
     1081 cggatgagca ggtaattcgg gatgagcgtc acggactccg gaatcatcat ggtcgccagg
     1141 aagaggccga acaggatgtc tttgccctta aagtctatcc ggccaaaggc ataacccgcc
     1201 agaaccgagg tgaaaagcaa gccagctaag gttcccagtg tcaccaaaac actgttcatg
     1261 aagtacttgg aaaaattagc ctgatcccag gcttccttgt aattcaccca ctggggaact
     1321 ttggggacca gttggcgcgt aatcgtctcg ccgagggtca tcagggaatt ggaaatcatc
     1381 cagaaaaatg ggaagataaa tatggccgcg cctatcaaca ggatgagata caggaggacc
     1441 ttcccgagga ttctgccggc gtggttttcc tttatttctt ttttgggttt ttgcattccc
     1501 gcagaattat ccatagaaaa cccgctctcc cataatcttg ttctgagcca aagtgagccc
     1561 aaggatgacg atgaaaagca cgatggcttg cgcggtggct accccgtact ggtttgcttt
     1621 gtagaagcgg tcaaatacca gcaagctggt ggtaatcacc gtattctgcg cgctgggcgt
     1681 gcgcatgacg taaaggtggt tgaaggcttt gaaagtaccg atgaaaccaa tgagcgtcag
     1741 gtaaaaagtc accggtgaaa tcatcgggat ggtaatgtgc cagaagcggt cccatttgct
     1801 gccgccgtca atttcggctg cctcgtaaat ttccttgggg atgtttccaa gccccgagag
     1861 gaagatgacg gtgttgtagc ccacgaacga ccagatgccg aaaatgatga tggtaaccag
     1921 cgccaggctg ggtcctccag ccagtccgct aacgttgaaa cccagcagct taagcacggg
     1981 tctgggctca aagcgccatt tcaaagggtc tatgccaaaa aagcctatca agctgttggc
     2041 cagcgaggtc tcccggctgg agaagatgat ttggaaaacg accgcggtac tgatggaagg
     2101 ggtgatatac ggcaggaagt agataatccg gaaagcctcc ttgccgacga tatcctgata
     2161 gaggatgtac gccagtacaa gtccaagaat aatctccgca ggaaccgtcc ccagcgcgta
     2221 atacacggtc acaatcaggg agttgaggaa atccttgttc ccggtttcgt acatcattcc
     2281 ccatccggtc acaaaaatca cgccgccgag gatgaggacg agcgaacccg cgatgtgggc
     2341 cagcttctga ccgttactga gagactggaa gccttttccc cacacaatcg aaccgagaat
     2401 aatcaacccc aaacctgcca gtaataccag tagattccac caggaaccaa agatgagcag
     2461 gtaattctta tcgccgatga agggcccctt ggtgacctgc cagttaaaca gactcatgta
     2521 aatggaataa ccaatcggga atatgccaaa aatcaaaatg atgatcagag cggggaggat
     2581 aaaaagataa ccggtgagtt gttcctgcag cttgataagc ttcagggtct tccggcgggt
     2641 ctggcgcact gcggtattct cagccaacga aacctccaac agccattgtg cattcggggt
     2701 tgattgccaa catcctcttt attttacagg ttttttttta tctgtgggtt ataaaaaggt
     2761 taacattggc ctttccctgc gaattctatc ttaaatccgg gatgaaaccc agaattgtct
     2821 ctggaaaact atccgtgaca gccaggaacc ccagttcgtc cagccgtacc aggccttccc
     2881 agttgcggga acttccatcc ggggtcagac tcaggtaaag cggcgcttcg tctgccatgc
     2941 ggatctgtcc gttttccaat tgcaagcgga gaatgcgttc gacgcgatcg gagcgtctgt
     3001 gcgacaagcc ttccccgaac ctttgggcaa tcgggtcgac ctccgccttt agatgcgcat
     3061 cgcccgggta aaaataattc ataacccaaa agctgccgtc cgcctgcgct tcggtcgcgt
     3121 ccgtgacccg atattcaatc gccggaaaat cagtttgagg ctgtgggctg agagcggtgt
     3181 cgaaaacatg tgcgtaagaa tacgggttga ctgcgctgcc gttgtcctcg aaaaaggtga
     3241 agagctgttc attcgcgacc acaagcgctt catcgctggc attccggaaa tccgtctgcg
     3301 gtaccaacgg aacaataccc tccgcctcca ggcggatgga ttggcgttcc tcatccacca
     3361 ctccggaaac aaggtagccc atcatcgggc tgccgccgct cgtctcaatc gtcaagaaga
     3421 ctttttctcc caggaacgca agcgcctcga aaccctcaaa cccttccagg ctttcagaaa
     3481 gtccaccgtc atcaaaggca ataagcccgg tttcgatggg ttccatgctc tccccgccca
     3541 ggtacgcgag gatgtcttcc ttttggatgg tcacgatgaa gccgctgccg tcctctccca
     3601 ggcggcgagg gtattgcggc agaagcacca gtttgtctcc cagccaggcc atcccggaaa
     3661 attccatccg ggcatcgtca tatggcgcgg gcagctttat gacccgcaca tctgcttcgg
     3721 ccggtttctc cgcagcagca gtctccgtcg gaactgtggt ttcggctgct gccgttggag
     3781 cgtcttcctt actgcttaat tccggagaaa catcacccgg caaggggaat tccgccttat
     3841 ttgtcggata gctacacgca ttcgtggaac atatcaagag tattatgcca aatatgctaa
     3901 tcaaccaggc tgacttgctt ttcatcactc gaccttaagc acagcatata ccgggtcatg
     3961 gtcgctcacc cgcgcgtcgg gcagcgcttc agaattgatg tgcacattgt caaagtgact
     4021 gaccaggctt aagcaaggaa ggctgaccag gatttggtcc aacacctggc cgttgccctc
     4081 atggttgtat gtgtagcggt cttgctccgg aagcagtgtg atcaggtttg ccaacgcgcc
     4141 atcctgaagc ttcataatcg gcgggctcca cgggaaatca ttcaggtcgc ccagtactac
     4201 gacatacgcc tctggattga taccgataat ttgcgctaca aattcgttga tctcgcgcgc
     4261 ctgttccatg cgctggcgtt cgctgacgag ctgcggcggc tgttggtcgc catagagcgg
     4321 gccgtcctcg cctttggaat tgaagtggca ggcgatgacg aaaatatccc ggccctggaa
     4381 gctgaattgc gcgctgagcg gtttgcggct gtcccggaag gcatatgctt gcgggctgac
     4441 ccggcccgga ttgagtgaga gcacaggctt tccgtctttc ttcagaacct gattggcgct
     4501 gctggcatcc ccagccgggg cggcagccag gcttaaccct ctgtcctccc tgtagagcag
     4561 cacaacccgg atattgcctc cggtcgctcc gccgtccgcg ttcctcagcg ggtcaattga
     4621 gagcgctttg taaaccggcc caccaacctg ttgtactgtc tggagcagcc tgttgatgtt
     4681 tccggcagcg gaagtctgcg cggaatcaag ctcgccgtca tcatcctgga tttcctgcag
     4741 ggccaggatg tccggactgc gcagaataaa aacaatttgt tccgccagcg cttccagcct
     4801 ttcaggcgag accatcgggt taaaattggc cacgttatac gtcgcgaaag ccaattcatc
     4861 ctctgagagc tcgtatgtaa ctgcctccgg cgggttattt ccctgctcaa aacgcaattt
     4921 ttgggtgggc tgcagcttga aattgccgaa actgtaatcg atgatcccga taataggctc
     4981 tgtaaaccgc gcgccggtgc gaatggcggg catgttgatg aatgtgtcgt caagaaggat
     5041 gcgttccggg ttggcgtcgt cttttcgcag gatgagcaca ctcctggggg ataataggcc
     5101 ggcatttttt cccatgtccg ctacaacagc gacctcttta taagaagagg tggaagaaac
     5161 tgccagggca gcgttcacct gaacgcgcat gccctccagg ctttcgtaaa aatccaaacc
     5221 gtcccgggca gggtcaaatt cgccgccctt actggcgtaa ccattcacat catcctcgat
     5281 aacactatcc ggaattctac ggcctccctc acccaacacc acgggttccg gcagcgaatt
     5341 gccgctggag agaattgtgt acgtaacctg gaatatttcc gtcctcgtca gggaattctc
     5401 gcccagcccg gctggattat actcacgcac tttgccttcc acaatccgca ccaaatcgcc
     5461 tgccttaaga tttccaaaag agctcgctga gacaaacagc gcctcggaag tgcgctcatc
     5521 gtcatcaggg tgcgggtctt gcaggtaaaa accgctgcct gtgagagcgg tgacaatgcc
     5581 ttccacgcca gagagctttt ttccgtcata aggggagcgg tgctgcgctc cctggacagc
     5641 gctgatgggc agtctgctgt ccacggctgc cgcggcgtcg gcagaagggg cgcccggcgc
     5701 ttggagtgag tttggccgca agcagcctga gagaatgaaa accaggcata aaaaggacag
     5761 gattgaactt ctctgttttt tcatcggcaa ccttagctgc tattgtaccc gttctgaacg
     5821 tccagtttgt aattttcgcg cgcccggaat gggttagaat taccgcatgg aaattcaaat
     5881 cgcgatcgca aaagtagacc ggtactcctc accagaacag ggcgatcaag tcgaaatggt
     5941 ggaaagaccc aacggcggcg cctcagtcat cctcggcgag gggaagttga atggtcaacg
     6001 gtcaaagttc gtggcgcgga aagcagtaca ccgcgtgctc tcgctcctgc tgaacggtat
     6061 tcacgatggc gcggcatccc gcgcggtact ctcagcgcta aaaacggaat accacggcga
     6121 ggcggagttc agcctgaacg tcctttcctg cgacctggaa agcgagacca tcgtcctcac
     6181 caaaaataac gacctgcccg taatcatgat tcgcaagagc gaaggcagct acaccagcta
     6241 tgaagggcgc gaccttgtgg acccgcagga gcccaatgtg tatcagttcc caatcgagaa
     6301 cgatttcatc atcatcatgt tctcggacgg cgttgccaca gccggttggg actgcagcca
     6361 accgctggag tggaactcgt tgatggagac cgtactggat gagcaagagc ttacggttca
     6421 ggagcttgcc gatcaaatcc tgaaccaggc ggtagcgcag gatttgggac agccgcatga
     6481 cgatatgtcc gtggttgtca tgcaggtcgg gagaactaca ggaaaaccca tacggcgaat
     6541 gtcactcacc ctgccaattt aaaccaagga gagcagtatg cgagaggaag ttgttctgat
     6601 taccggagcg aacggagaaa tcggccacgg cctgatccgc cagatttccg ccatgaattg
     6661 tccgattgtc gccatcgacg tcaatccgct ggataagccg ctgcgctcca tggtcgcgaa
     6721 atggattgtg ggcgatatcc tggaccaaat cctgttgggt cggctggtcg tcgagcatca
     6781 gattcggacg atttaccacc ttgcttccat tctctctacc aaagccgaat acaacccgga
     6841 aaccgcccac cgcatcaatg tggaaggaac cctgaatctg ctgcgcctgg cggtcgaaca
     6901 atcgcagtgg cagggcaatt cagtcaagtt catttacccc agctctattg ccgcctacgg
     6961 aatggactcg ctggactgca aagcccggtt gggcaaggtc aaagaggatc aatacacttt
     7021 cccgaccacc atgtacgggt gcaacaaact gtactgcgag caccttgggc gttactacag
     7081 caagtattac cgccagttag ccgcggactc tgcttccggc ccgtcggtgg atttccgctg
     7141 cctgcgcttt ccgggcctca tcagcgcgga gaccatcccc accggcggaa ccagcgatta
     7201 cggccctgaa atgctgcacg cggctgccaa gggcacgcct tacaactgct ttgtccgccc
     7261 ggatacccgc ctgccgttca tggtgatgcc ggacgcgatt aaatccctga ttactctcga
     7321 gaatgccggg cacgaaaaac tcacccggtt tgtctacaac gtcaccagtt tcaatccaac
     7381 cgcgcaggaa attttcgaaa taacgacccg ggctttcccc ggcgcgcgca tcgggtttga
     7441 agtagaccag cgcaggcaaa aaatcgtcga ttcctggccg gaaaacgtag acgacagcgc
     7501 tgcccgccag gattggggct ggcagcccga ttacgaccag gaacgcgcct tcgaagaata
     7561 tctgataccc gccatccgca atcagtatac cgagcttgcc aacccgtaag atccggttta
     7621 ctattctgac catccagcat ccgagccaaa gttgaggaac gcttatgcca atctatgaat
     7681 atcagtgccg gcaatgcggc gcacattttg aactccttcg ttcatataac caatctgatt
     7741 ccccggcgga gtgcccgcac tgctccggct cggagacccg gcgcgaactt tccagagtga
     7801 acgctttcag cggcggcaaa agcctgaccg actccggcca ttcctgcggc ggctgctctg
     7861 gcggcagctg cagcacctgc ggacattagc catgaaaaac ctgaacggaa aacttgcctt
     7921 aattaccggc ggctccagcg gcattgggct ggcgcttgcc agcaaactgt tcgccagcgg
     7981 aatgtcggta gccatcctcg cgcgcgggca gcagcggctg gatgaagcgc tcgcaattat
     8041 tgagaaatcc cggctgaacc ccgctcagaa gcttacggcc atttctgcaa acgtcgcgga
     8101 cgcagcagga atcgcacagg ttttacggga attcgttgcc ggttatggac tgcccgacct
     8161 ggtcatcaac tgcgcgggcg tcgccaaatc cggctacgcg gaagcgaccc cgcttgaaga
     8221 cttccgctgg atgatggaca tcgattatca cggcacggtg aacgtggtgc agaccctcct
     8281 tccagagttt atcgcgcgcg gaagcgggca tatcgtaaca atttcctcgc tggcaggggt
     8341 tgtaggggtg tttggatata ctgcctacgg cggcgccaag ttcgcggtca gaggttacac
     8401 cgacgcgctt cgcgcggaaa tgaaatcgcg cggcatccag gtttcgtgcg tctttccgcc
     8461 ggacacagac accccgcagc tctcctatga aaatcaattc aaaccctacg aaacgcatgt
     8521 cattgccggt tcggataaac cgctctccgc ggaaaaggtc gccgatagta ttgtgcgcgg
     8581 tatccaaaag gacaaatatg caatcgttcc gggattggaa gccaaggttc tattcttcct
     8641 ggtaacgcat ttgggagggc tggtttaccc tgtgctcgat ctgctggtga agagcgcgtc
     8701 aaaaaagaaa gcggcgcttc aggttgctca aacggaaaag cgcggtcggt agactggcag
     8761 catggaaata gatgaactcc taaacctgtg gaagagagac ccggagttta tccgcaatac
     8821 cacctgttgg caggttctcc cccccaccgg agcagtttac gaagctctgc ccaacaatct
     8881 tccggagcag ctgcgctgca tcctggtcaa tcggggtatt acgcgcctgt tcagccatca
     8941 agcccagtcg ctcgcccatt tatccgcggg cgaacacgtg gtcgtcagca ccggcacagc
     9001 cagcgggaaa accctttgtt atgacatccc cgtcatcgag acgcttctgc agaaccctga
     9061 cgcgcgcgcc ctttacctgt tccccaccaa agcgctggcg caagaccagt tccgcctgct
     9121 gaacgacctg tgcgcctcgc ccggggggaa cctgaaaccc ctccccgcca tttacgatgg
     9181 ggacacgcct tccaatcacc gggctgaaat ccggcgcaag gctgaaatcc tcatcaccaa
     9241 ccctgatatg ctgcatcagg gcatcctgcc gcatcacacc atctggcgcc agtttttcgg
     9301 ggggctcagg ttcgtggtga ttgacgaaat gcatacctac cgcggggttt ttggctcgca
     9361 tgttgccaac gtcatccgcc ggctcaaacg agttgctgcg ttttatcagg cttacccgca
     9421 gttcatcctc acctcggcta ctatcggtaa cccggaagaa ctggccgaaa agctgattga
     9481 aagcccggtc gcattggtcg atcaggacgg ctctccccac ggccgccggc acttcctcgt
     9541 gtacaacccg ccgctggtca acgaacagtt gggtttgcgc gccagcagcc tgcaaacggg
     9601 tatttccttt acccgcaagc tgttgagcaa cagcatccaa acgctggtct ttgcccgttc
     9661 ccgccgcagc gtcgaaatga tattgacgta cttgtacgat gccgtgccag gggaaacgcg
     9721 cgcacgcatc cgtggatacc gctcgggata tctcaagagc gagcggcgcg aaatcgaagc
     9781 cggcttcaag gacggttcca ttaaagctgt ggtcgctacc tccgcgctgg agctcggaat
     9841 cgacatcggc agcttggaat cggtcatcct ggtggggtac ccgggttcca tcgccaccac
     9901 gcgccagcgt gccggcaggg cagggcgccg acagcaggct tcgctggcga tgtttattgc
     9961 ttccccggaa gcaatggacc aatacctggc caaccacccc gaatacctca cagaaaaatc
    10021 cccggaaaac gccctgattg acccgaataa ctatgccatt ctgttgcagc atctgcagtg
    10081 tgccgccttc gagctgccct tcctggaaaa cgaccgcttt ggcagcctgc cgccagaact
    10141 gttggattca tttctgaaaa ttctgacgca atccggagta ctccaccttc agaacggccg
    10201 ctatttctgg attgcggacc aatttgctgc cggcagcgtt tctttgcgca gcagcacgcc
    10261 caacgtaatt accttacgcg ctgcggacgg ggataccacg catgtcatcg gggaaattga
    10321 cgctgccagc gccaaatggt tggtgcatcc ggaggcaatt tacctgcacg aagcggattc
    10381 ctacgaggta aaagcgctgg acctggagaa cggcagttgc ttactcgagc cggtgcagtc
    10441 gg
//
LOCUS       DAOQAD010000065        10242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_7623_length_10242_cov_5.015019, whole genome shotgun sequence.
ACCESSION   DAOQAD010000065 DAOQAD010000000
VERSION     DAOQAD010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7623_length_10242_cov_5.015019"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3353)
                     /locus_tag="PKL60_06180"
     CDS             complement(<1..>3353)
                     /locus_tag="PKL60_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNW95756.1"
                     /translation="VSDSAAAQFAETVPVPVNEESEAENAVTMLASVPDAAAAQSAET
                     VPLPMNEADQAENAQTVPLSVPDSAEAQPAEAPAQPGSPETKREATQPLEIAKTAAPE
                     PTAWFSEIASYDEKVGSAPKAPAPKPAQPRGDFFTGDWVRPKPAQTAPTGTWNTKPTG
                     AWIPPAAPQDNIPTIPPAVSVPPLASGGQPLPIKKVTETDPGATRLSPSAFSQRQASK
                     AQGAQPAPANGRQAPMDKAQTRRSPVQAKPSQAAPAKKKAAKALLPVLFVILALIFVG
                     MLFVGVQYFRIASTLPSVAELRSRASQFETTRILDRRGNILYEINDPNMGKRTYVPLD
                     KISPYLIAATIATEDKEYYNHPGFDLVALVRALWTNYTSGEIVSGGSTITQQLARMLL
                     LQEERFEQTYDRKAREIILAAEITRRYSKEEVLELYLNEIFYGNLSYGIEAAAETYFN
                     TPASDLQLWQASFLAGLPQSPAIYDIYNNRDATLTRNKAVLVLTYELSNEKNCIYIGE
                     NLEKVCVSANDALNAADTLEAYPFTQNTNSMRFPHWVVYIQSLLESQYDSQTIYHSGF
                     TIYTTLDPELQDLAQRAVQEQLATMTANNATNGALVAMDPNTGEILAMVGSADFYNDA
                     ISGQVNMALTETRQPGSSIKPITYLAAFEKDWTPSTLIWDVPGSFPPSGREDDPSAPY
                     EPVNYDGRFHGPVTVRSALANSYNIPAVKTLQYVGIYDDPNTNSEDGFIAMAKRLGIT
                     SLTREDYGLSLTLGGGEVSLLQLTGAYSVLANEGKRVAPVAITKVVDYTGKVVFEYSA
                     PEGDQVVRAAHAYLITSILSDANARAPMFGRDSILNLPFAAAAKTGTTNDYRDNWTLG
                     YTPDLVVGVWVGNADYSPMVNTTGISGAAPIWARVMEDGINLLKGGAATPFTQPQDVV
                     ERTICSASGTEPSDYCPSQRTEIFASDQLPPSKEHDLWAKVQIDGWTGLRASAACSDF
                     AREELVVNVSDKDAVRWLKTDPGKNWAQDNGFPDPFVILPERECRADDPRAVIDMVTL
                     TDGRTITENPFKIVGVIDATANFKEFKIDWGEGEKPGEWNTLVDWENTPVRSAEEIYS
                     WDLTGVTAEIVTVRIRLRSTVDTQVEKRFT"
     assembly_gap    3354..3453
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3454..3891)
                     /locus_tag="PKL60_06185"
     CDS             complement(<3454..3891)
                     /locus_tag="PKL60_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95757.1"
                     /translation="MRKNDADPDDTGRNGFGELNSFQDFDNPDRTPPSAEQDTQETTV
                     PGIPAADLEAQARTEKLKPGADSSESSTEDAESLTEDLPEDAAEPSPIKAGQAENAAT
                     VPVPALDSAAAQNAETLPIPIHPEAELESAPTVLVSVSDSAAAQ"
     gene            4054..6405
                     /locus_tag="PKL60_06190"
     CDS             4054..6405
                     /locus_tag="PKL60_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95758.1"
                     /translation="MSAGPFFSSDPILLTLAADQRFSTLNALVDAVWQIHLKPIGPLS
                     LETSFGLQAERFRIFPVFLVNNKAIAEINAYLSPPRVDFIYANYASLSCSPAEGTQAR
                     CEFWAAESDTLLTRFTLTNTSPAALKLGVQSAAELCPREGGPGMNPTALQYQPYLLGR
                     SGSLFVAVAHAQAAQVVSSPYPALQRSEELAPGESLSVQQRCCAGSEQARVEKKLFQA
                     YPENWDAQIARLSLRHQASLLRVSTPFADWDAVFLSAQNQAFQLLARSGDDQEALDDL
                     NFRSPEYSYAQYTNGKDPSAVLLPPFNALSLYQLIQALLPVQAQTAAWIFARRLASSP
                     NCSRLPVNAREVLPFPCLCALALSIFLQTGDRDFLAAVYPRLCQSTLAWFDDDHDLDQ
                     DGLPEWLTFSQTGWHDHPSFNFLNPHGLFTWITTAESIGLSCLLKRELDALEQMARVL
                     EDEETAAQAAIRKRKLNAGLEQIRKELPEASTWDRDTHLSTPQKLLYEGAFADIPAGL
                     VFEPPSRVNLKLILSAFTNKPALIRLRGTDADGKQMEERIEAGEILRLADSLALTSNQ
                     VFARLESVSAQGLDEKTWTQLYTADLTLRDIGWYLAWQPAALPADDEPDEQNADESLF
                     QAFPHGLPEIISETAGGNAGLWVNPAWNTLVLEHLIQIGAKKEASALFANLMRGAAGV
                     LKREHAHFEAWRAADNQPVGSRGAAAGLIPLHAFLELAGVRILAADKVQITGEYPFEW
                     PLTLRYQGLEITREGKNSSITMPDGTLFHHFGSQPKTFQSERD"
     gene            complement(6436..8223)
                     /gene="glmS"
                     /locus_tag="PKL60_06195"
     CDS             complement(6436..8223)
                     /gene="glmS"
                     /locus_tag="PKL60_06195"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNW95759.1"
                     /translation="MCGIFGIVTQNEQNLGPTLVEAARRLTYRGYDSVGAAVISGSQI
                     DLRKDKGRVDEVAEKYRFAEMNGQRGIAQLRWATFGVPSQVNAQPHLDSDGDLVGAHN
                     GNVVNNVELRQEFTAEGMTVRSQNDGESCVHAVERYIDRGYGFVDAIRAAYADLQGDY
                     AFVIGRINDDKLYAIKKGSGLVVGIGEGFTCVSSDLPSILPLTRKVLRLNDGDVVCLS
                     WDRVALYSVADGSLIAREPEIITEEMSAVQKGGYAHFMLKEIHEQPQVARELLHLLNR
                     HEDLDRILEAIRGARNLYLIGCGTSYHACLAGSVYFARLAGRAAVPVLAPQFIPQYAP
                     TVGPQDVGIFVSQSGETKDVLNALEQAQKRGMTCFSMANVIGSTLTKITPGWLPLTCG
                     YEISVPATKTFTNQVISFLFLAMMLRGGGAESLNGLPELMEQAIRWTDPQARALAEDI
                     NGWNDLYSLGYGSTYAMALEGALKLKEITYAHCEGMLSTEFKHGPLSAVTDGFPVLFA
                     VGPEDVPLIVSGINEVTCRGGRAIAIGEEDSRLNANASDVITIPPSNPLFAPLLAVIP
                     LQLLSYYMAVGRGYDPDFPRNLSKTLTVD"
     gene            8289..9143
                     /locus_tag="PKL60_06200"
     CDS             8289..9143
                     /locus_tag="PKL60_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95760.1"
                     /translation="MESHPNCRCTSTAITKSWEELGAEFGFDFSAAEKAGPSFAEVAD
                     KYGVTPEQRRAYANRKGSGEAYFKNLDPVEQQSVLGKTRWQAWSTGKLSFDNMIQKTS
                     NPVWGVTQKLSSLSSLGLEENKLFEEFKSDQSKTVHNAAVESIKINKVKGGSSVSLIY
                     GDMAGKPYYSVSIFPECGLSIPGKEIDEEGLQSYIREHMDLLNRRNIVIGTWYNERDG
                     RTYIDISAIISDENIAIELGKRYNQIAIFNLEDAREVFTGGTGENISSLPSIEKRLKE
                     LKYGNPIN"
     gene            9622..10110
                     /locus_tag="PKL60_06205"
     CDS             9622..10110
                     /locus_tag="PKL60_06205"
                     /inference="COORDINATES: protein motif:HMM:NF021602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HNW95761.1"
                     /translation="MEKTLDRLFQMSRRRRSNQSKQLTTQNNNRPSRNIERGAEIIHQ
                     EAHFFNGPIPPAEELEKYSDIIPDGANRIMLMAEQQAQHRIELENKVINNDISNSRLG
                     MIFGFILQLVAIVGSVIIAITQSMWEGMLMAIGVIVIGIVNLRLAKQDREKELSRHKD
                     VQ"
BASE COUNT         2122 a         2845 c         2974 g         2201 t
ORIGIN      
        1 gtgaacctct tttccacctg cgtgtccacc gtgctgcgca gccggatgcg aacggtcaca
       61 atttccgctg tgaccccagt caaatcccag gaatagattt cttccgcgga gcgcaccggg
      121 gtgttttccc agtccaccag ggtgttccat tccccgggct tctcgccttc gccccagtca
      181 atcttgaact ccttgaaatt cgcggtagcg tcaatgacgc cgacaatctt gaagggattc
      241 tcggtgatgg tgcgtccgtc tgtgagggtg accatatcga tgaccgcgcg ggggtcgtct
      301 gcccggcatt ctctttccgg caggatgaca aaggggtccg ggaagccgtt gtcttgcgcc
      361 cagtttttcc ccgggtcggt cttcagccag cggactgcgt ctttgtcgct gacgttgacc
      421 accaattcct ccctggcgaa gtcggagcag gccgcggaag cccgcaagcc ggtccaacca
      481 tcaatctgca ccttcgccca caggtcgtgc tccttggagg gcggcagctg gtccgacgcg
      541 aagatttccg tgcgctggct ggggcagtaa tccgagggtt cggtgccgga agcggaacag
      601 atggtgcgtt ccacgacgtc ttgcggctgg gtgaacggcg tggcagcgcc gcccttgagc
      661 aggttgatgc cgtcttccat cacgcgcgcc caaatcggcg cggcgccgct aatgccggtc
      721 gtgttcacca tcggggagta atccgcgttg ccgacccaca cccccaccac caggtcgggg
      781 gtatagccga gggtccagtt gtcgcgatag tcattggtgg tgccggtttt tgccgccgcc
      841 gcgaagggca ggttcaggat ggaatcccgc ccgaacattg gcgcgcgcgc gttggcatct
      901 gaaaggatgg aagtaatcag gtaagcatgc gccgcgcgca ccacctgatc gccttcgggc
      961 gcgctgtact caaacactac ttttccggtg tagtccacca ccttggtaat cgccacggga
     1021 gccacgcgct tgccctcgtt cgccagcacc gagtacgcgc cggtcaattg cagcaggctc
     1081 acctcaccgc cgcccaacgt gagggaaaga ccgtaatcct cgcgggtcag gctggtgatc
     1141 cccaggcgtt ttgccatggc gataaagccg tcctcgctgt tggtgttcgg gtcgtcgtag
     1201 attcccacgt actgcagcgt ttttaccgcg gggatgttat aggaatttgc cagcgccgag
     1261 cgtacggtca ccggtccgtg aaaacggccg tcgtagttga ccggttcgta aggcgcactg
     1321 gggtcgtctt cccgccctga agggggaaag gaacccggaa cgtcccaaat caaggtcgaa
     1381 ggggtccagt ctttttcaaa ggcagccagg taggtgatgg gcttgatgga cgagcccggt
     1441 tggcgcgtct cggtcagcgc catattcacc tgcccgctga tggcatcgtt gtaaaaatcc
     1501 gcggagccca ccatcgccag aatttcccct gtgttcgggt ccatcgcgac cagcgcgccg
     1561 ttggtggcgt tgttcgcggt catggtcgcc agttgttcct gcaccgcgcg ctgggcaagg
     1621 tcctgcagct ccgggtcgag cgtggtgtaa atagtgaacc cggaatggta aatcgtctgg
     1681 gaatcatatt ggctttccaa cagagactgg atgtacacaa cccagtgggg gaagcgcatg
     1741 ctgttggtat tctgagtgaa gggataggct tccagcgtat ccgccgcgtt cagcgcgtcg
     1801 ttggcgctga cacatacttt ttccaggttt tcgccgatgt agatgcagtt tttctcattg
     1861 ctcaattcgt aagtcagcac cagcacggct ttgttgcgcg taagagtggc gtcgcggttg
     1921 ttgtagatgt cataaatcgc aggggactgc ggcagcccag ccaggaagga cgcctgccaa
     1981 agctgtaagt ccgaggcagg ggtgttgaag taggtttccg ccgcggcttc gataccatac
     2041 gagaggttgc cgtagaaaat ctcgttcaga taaagttcca gcacctcttc cttgctgtaa
     2101 cgacgcgtta tttcagccgc caggatgatt tcacgcgcct tgcggtcgta tgtttgttca
     2161 aagcgttctt cttgcagcag cagcatgcgc gccagctgtt gggtgatggt ggacccgccg
     2221 gaaacaattt ccccggaggt gtaattcgtc cacagcgccc ggacgagcgc caccaggtca
     2281 aagccgggat ggttatagta ttccttatct tcggtcgcga tggtggcggc aatcagatag
     2341 ggcgagattt tatcaagcgg cacataggtg cgcttgccca tgttggggtc gttgatttca
     2401 tacagaatgt tgccgcgcct gtccaggatg cgcgtggttt caaattgcga ggcgcgcgaa
     2461 cgcagttccg ctacgctggg cagcgtggag gcaatgcgga aatactgcac gcccacgaac
     2521 agcatcccca caaaaattag agccagaata acgaagagta ccggcaggag cgctttggca
     2581 gctttctttt tagccggcgc tgcctggctg ggtttagcct gaacggggga gcgcctggtt
     2641 tgagccttgt ccattggggc ttggcgccca ttggcgggcg ccggctgagc gccttgggct
     2701 ttgctcgcct gccgctgcga aaaggcggag ggcgaaagcc gggttgcgcc ggggtctgtc
     2761 tcggtcacct tttttatagg aagcggctgc ccgccggagg ccaacggtgg cacagaaacg
     2821 gcaggcggga tggtcgggat attgtcctgc ggggcggcgg gtggaatcca ggcgccggtg
     2881 ggtttggtgt tccaggtgcc cgttggcgca gtttgggcgg gcttgggccg tacccaatcc
     2941 cccgtgaaga aatccccgcg cggctgcgca ggcttgggag cgggggcttt tggcgcgctg
     3001 ccgaccttct cgtcatagga agcgatttcc gagaaccagg cggtaggctc cggcgcggca
     3061 gttttcgcga tttcgagcgg ttgggtggct tcccgtttcg tttcagggct tcccggctgg
     3121 gctggcgctt ccgcgggctg cgcttcagcg gaatccggaa cggataaggg tacggtttgg
     3181 gcgttttccg cctggtccgc ctcgttcatg ggtaggggaa cagtctcggc agactgcgcg
     3241 gcagccgcat ccggcacaga cgccagcatg gtcacggcgt tttccgcttc gctttcctcg
     3301 ttgacaggta ccggtacagt ttcggcaaac tgcgcggcgg ccgaatccga tacnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctgcgcg gcggccgaat ccgatacaga
     3481 caccaacaca gtcggtgcgg actcaagttc cgcttccgga tgaatgggta taggaagtgt
     3541 ttccgcgttc tgagcggcag ctgaatccag cgcgggcaca ggcacggtcg cagcgttttc
     3601 cgcttgtcct gctttaattg ggcttggttc agcggcatct tcaggcaggt cttcagtcaa
     3661 gctctcggca tcctctgtgg acgattcaga actgtccgcg ccgggcttta gcttttcggt
     3721 tcgcgcctgc gcttccaggt ccgccgctgg gatacccgga acggtggtct cctgggtatc
     3781 ctgttctgcg gaaggtgggg tgcggtccgg attgtcaaag tcttgaaagg aattgagctc
     3841 gccaaaacca tttcgcccgg tatcatccgg gtctgcgtcg ttcttcctca tagggtcttc
     3901 tttcatggcg ttattgtagc ataaaggcat tttacccatt gacgccggca gcccccggta
     3961 tgttcccggc tgcagcctat acgggcagcc cgggttttca cgagcccgcc gtggaagcgc
     4021 ggagattgcc cgggtagtgc taaaataatg cgcatgagcg ccggtccctt cttttcctca
     4081 gatcccatac tgctgactct ggcagcggac cagcgctttt ccacgctaaa cgcgttggtg
     4141 gatgcggtct ggcaaatcca tctgaaaccg attggaccgc tttcgttgga aaccagtttc
     4201 gggctgcagg cggaacgctt ccgcattttc ccggtcttcc ttgtgaataa caaagctatt
     4261 gcagaaatca acgcgtacct ctccccaccg cgggtagact tcatctatgc caattacgcc
     4321 agcctcagct gttccccggc tgaaggcacg caggcccgct gtgaattctg ggctgcggaa
     4381 agtgacacgc ttcttacacg cttcacactc acgaacacca gtccggctgc gctgaagctt
     4441 ggcgttcaat ccgcggcaga actttgcccg cgggaaggcg gccctggcat gaaccccacc
     4501 gcgctccaat accagcccta cctcctggga cgcagcggca gcttgttcgt cgcggtcgcg
     4561 cacgctcagg ctgcacaagt tgtatccagc ccttatccgg cgctgcagcg cagcgaggaa
     4621 ctggctcccg gagagtctct ttcggtgcag cagcgctgct gcgcgggttc tgagcaggcg
     4681 cgggtggaaa aaaagctgtt tcaggcttac ccggagaact gggacgcgca gattgcgcgg
     4741 ctctcgctgc gccatcaggc aagcctgctg cgcgtgagca ctcccttcgc ggattgggac
     4801 gcggttttcc tctccgccca aaaccaggct tttcagctgt tggcgcgctc cggggacgac
     4861 caggaagcgc tggatgattt gaatttccgc agccccgaat acagctacgc gcagtacacg
     4921 aacggcaaag acccctcagc cgttttgctg ccgccattca acgctctttc actctaccag
     4981 ctcattcagg cgctgctgcc ggttcaggcg cagaccgccg cctggatttt tgcccgccgg
     5041 ctggcctcca gcccaaactg ctcccggctg ccggtgaacg cccgcgaagt gctgccgttc
     5101 ccctgcctgt gcgccctcgc cctcagtatc ttcctgcaga ctggcgaccg ggacttcctt
     5161 gcggctgtct acccgcggct ctgccaatcc acccttgcct ggtttgatga cgaccacgac
     5221 ctggatcagg acggactgcc ggaatggctg acgttctcgc agaccggctg gcatgaccac
     5281 cccagtttta atttcctcaa cccccacggg ctttttacct ggattaccac ggccgaaagc
     5341 atcggcctga gctgcctgct gaaacgggag ctggacgccc tggaacagat ggcgcgcgtc
     5401 ctggaggatg aggaaacagc cgcgcaagcc gccatccgca agcgtaagct gaacgcgggg
     5461 ttggagcaga ttcgcaagga actgcccgaa gcttccacct gggaccgtga tacccacctt
     5521 tccaccccgc aaaagctcct ttatgaaggc gccttcgcgg atattcccgc cgggctggtg
     5581 tttgagccgc ccagccgcgt gaacctcaaa ctcatcctga gcgcgttcac gaataaaccc
     5641 gctttgattc gtttgcgcgg aaccgacgcc gatgggaagc agatggaaga acgcatcgaa
     5701 gccggcgaaa ttttacgcct ggcggacagc ctggcgctca ccagcaatca ggttttcgcc
     5761 cggctggaaa gcgtgtccgc tcaaggactg gacgaaaaga cctggaccca gctctatacg
     5821 gctgacctta cgctcaggga tatcggctgg tatttagcct ggcagcccgc cgccctcccc
     5881 gcggatgacg aaccggatga gcagaatgcg gacgaaagct tatttcaggc ctttccgcac
     5941 gggctccctg agataatctc tgaaactgcc ggaggaaatg ccggtctgtg ggtcaaccct
     6001 gcctggaaca ccctggtgct ggagcatctt attcaaattg gcgcaaaaaa agaagctagc
     6061 gcgctcttcg ccaatctgat gcgcggcgcg gcgggcgtat taaaacggga acacgcgcat
     6121 tttgaagcct ggagagccgc ggataaccag ccagtgggca gccgcggcgc ggcagccggg
     6181 ctcatccccc tgcacgcatt cctcgaactg gcgggcgtgc gcattttggc ggcggacaaa
     6241 gtgcagatta ccggcgagta cccctttgag tggccgctga ccctgcgtta ccagggtttg
     6301 gaaatcaccc gcgagggcaa aaacagcagc atcaccatgc cagacggcac gctcttccat
     6361 cattttggca gccagccgaa aacatttcaa tccgaaagag actgaaattt catccaagcc
     6421 tggcctggcg gggaattaat ccacggtcag cgtcttgctc aagttgcgcg ggaaatccgg
     6481 gtcgtagccg cgcccgaccg ccatgtaata cgaaagtaac tgcagcggaa tcaccgccag
     6541 cagcggcgcg aagagtggat tcgagggcgg gatggtaatc acatcgctgg cattcgcgtt
     6601 cagacggctg tcttcctcgc caatcgcgat tgcccgccca ccccggcagg tgacctcgtt
     6661 tatcccgctc acgatcaacg gaacatcctc cggaccaacc gcaaagagca ccgggaaccc
     6721 gtccgtgacc gccgagagcg gcccgtgttt gaattctgtg ctaagcatcc cctcgcagtg
     6781 ggcgtaggta atttccttca gtttcagggc gccttccaac gccatcgcgt aggtgctgcc
     6841 gtagcccaga ctatataggt cgttccagcc gttgatgtct tccgccagcg cccgcgcctg
     6901 cgggtctgtc cagcggatgg cctgttccat cagctccggc agcccgttca gggattccgc
     6961 cccgccgccc cgcagcatca tcgcgaggaa caggaagctg atgacctggt tggtgaaggt
     7021 cttggtcgcc ggcacgctga tttcgtagcc gcaggtcagc ggcagccagc cgggggtgat
     7081 tttggtgagc gtggagccaa tcacattcgc catgctaaag caggtcatcc cccgcttttg
     7141 tgcctgttcc agcgcgttca agacgtcttt cgtctccccg gactgactga cgaaaatgcc
     7201 cacgtcctgc ggtccgacgg tgggggcata ctgcgggatg aactgcggcg cgagcaccgg
     7261 tacggctgcc cggccagcca gccgcgcgaa atacaccgaa cccgccaggc aggcgtggta
     7321 actggtgccg cagccgatca ggtagaggtt gcgtgccccg cggattgcct ccaggatgcg
     7381 gtccaggtct tcgtggcggt tcagcaggtg cagcagctcg cgcgccacct gcggctgttc
     7441 gtggatttcc ttgagcataa agtgcgcgta tccgcctttt tgcacggcgg acatctcttc
     7501 cgtgataatt tccggttccc gcgcaatcag gctgccgtcc gcgacggaat acaaggccac
     7561 cctatcccag ctcaggcaaa ccacatcccc atcgttcaag cgcagcactt tgcgggtgag
     7621 cggcagaatc gaaggcaggt cggaggatac gcaggtgaag ccctcgccga tgccgaccac
     7681 cagcccggag cccttcttga tggcgtagag cttgtcgtcg ttaatgcgcc cgatgacgaa
     7741 ggcgtaatca ccctgcaggt cggcatacgc cgcgcggatg gcatccacga agccgtagcc
     7801 gcggtcgatg tagcgttcca ccgcgtgcac gcaggactcg ccgtcgtttt gggagcgtac
     7861 ggtcatgcct tccgccgtga attcctgccg aagctcgacg ttgttcacca cattgccgtt
     7921 gtgcgcgccg accaggtcgc cgtccgaatc aagatggggc tgggcgttca cctgggaagg
     7981 cacgccgaag gtcgcccaac gcagctgcgc gatgccgcgc tggccgttca tttccgcgaa
     8041 gcggtacttc tcggccacct cgtccacgcg gcccttatcc ttgcgcaggt caatctggct
     8101 gccgctgatg accgccgcgc cgaccgaatc gtatccgcgg taggtcagcc ggcgggcagc
     8161 ctccaccagg gtgggcccca ggttttgttc attttgggtc acaatgccga aaattccgca
     8221 catatttgtc ctttttgcag ttagtctgtc gaggctgaat tatagcggaa aattccagag
     8281 tattcgtgat ggagtcacat ccgaactgcc gctgcacgag cacggcgatt accaagagct
     8341 gggaggagct gggcgctgag ttcgggtttg acttcagcgc ggctgaaaaa gcgggtccgt
     8401 ctttcgcgga agtggcggac aagtacgggg ttacgccgga gcagcggcgg gcgtacgcga
     8461 accggaaggg gagcggggag gcgtacttca agaatctgga tccagtagaa caacagagcg
     8521 tcttagggaa gacaagatgg caggcgtgga gtactggaaa actttcattc gataatatga
     8581 tccagaaaac ttctaatcct gtttggggag taactcaaaa actctcatca ttaagttcat
     8641 tagggctcga agaaaataag ttgtttgagg agttcaaaag tgatcagtca aaaactgtac
     8701 ataatgcagc agtcgaatca atcaagatta ataaagtaaa aggtggatct tctgtcagcc
     8761 tgatctacgg cgacatggcg ggaaagccat attactctgt gtcaatattt ccagagtgcg
     8821 gtctctcaat cccaggaaaa gaaatagacg aagaaggcct gcagagttac atacgagaac
     8881 acatggatct tttgaacagg aggaatatcg taattgggac gtggtataat gagcgagatg
     8941 gaagaaccta tattgatatt tctgcgataa tttcagatga gaatattgcg attgagttgg
     9001 gaaagagata caatcagata gcaattttca atctggaaga cgccagggaa gtttttactg
     9061 gcggaacagg cgagaatatc agttcattgc catcgattga gaaacggtta aaagagctga
     9121 aatatggtaa tccgatcaac tgaagtattt gatttcaatg gtcccttcgg gagcgattac
     9181 ggtttgctta tgtcaaaggt ttttgaggct acgcttgatg gtggcaaaac acactttaac
     9241 aagcatttca gctgcgtggt aaaagcacaa aacaagcacc agtatgacct gtttctacat
     9301 atggtcaagg tgggtgacaa ggtagagatt agcacggctt caaaaggtta cacaacaccc
     9361 actcttgagt ccttccggcg cgtctaaaca cctgagtagt attagacgga aacaaaaaca
     9421 aaaaatggaa caactggata ttgcttttcc agagatcggg tgaaaatatg actgatgacg
     9481 gttttagact ttacgcaagg ccttcgttct tcgagggatt ggcccgaata tgggatccgg
     9541 ctggaatact gaaccaatat aaccgatcaa agaccgctaa ggatgcggac cggaaagcca
     9601 tagctcaaga ctgggcagtc gttggaaaag acattagatc ggttgtttca gatgtcaaga
     9661 cgaagaagat ccaatcaaag taaacaacta actacgcaaa ataacaaccg cccttcaaga
     9721 aatattgaac gaggggcgga aattattcat caagaagcgc atttttttaa tggccctatc
     9781 ccgccagctg aagaactcga aaagtatagt gatattatcc ctgacggtgc aaatcgcatt
     9841 atgttgatgg ctgaacaaca agcacagcac cgaattgagt tggagaataa ggtaataaat
     9901 aatgacatat ccaattcaag gttgggaatg attttcggat tcatccttca gttggtggca
     9961 atcgttggaa gtgtaattat cgcaataact caatctatgt gggagggaat gctgatggcg
    10021 attggggtaa ttgtgattgg tattgtgaac ttacgcttag caaagcagga ccgcgagaaa
    10081 gagctaagca gacataaaga tgttcaataa ctctcagccc gcctcttccc ccaggggcgg
    10141 tgtgctttaa ggggtgaaag caccaaattc agacaatctt tgcggacaca atctttgcgg
    10201 atatcttgca atattagatt ctttgtgcta aactactaat aa
//
LOCUS       DAOQAD010000066        10019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_7874_length_10019_cov_6.198615, whole genome shotgun sequence.
ACCESSION   DAOQAD010000066 DAOQAD010000000
VERSION     DAOQAD010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10019
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7874_length_10019_cov_6.198615"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..558)
                     /locus_tag="PKL60_06210"
     CDS             complement(43..558)
                     /locus_tag="PKL60_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNW95762.1"
                     /translation="MSVTSTSKYAYIECDPNLCVGCQLCEYLCSFSKTGEYNSFRSRI
                     RTIRVDEVLISAIACRTCENAPCVIVCPRDAMTQDPDKGIIRVDSTKCDGCAWCVEAC
                     DFGAISINPATALAEICDQCETDPEGPQCVKWCPKEALTLTTAEQRTQRARKKLIAET
                     LDIPTKAVEPK"
     gene            complement(570..2456)
                     /locus_tag="PKL60_06215"
     CDS             complement(570..2456)
                     /locus_tag="PKL60_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNW95763.1"
                     /translation="MYGYSGKILHIDLRTHKTRIEEKPEEWYKLWIGGVSMASRLLWE
                     NIVPGCDPLDDGNPICFANGIFTGTPVPVGGKFGMASKSPLTGFIGDSLSGSWFSIAL
                     KRAGWDGVVLHNSCENWTHLFIDDDRVQFFDATYLLGKGTFETEEAIREKMGDEQIRS
                     CTIGICGEHLVRYANVTNDGRQLGRTGHGMIWGHKKLKAFSVRGTKGVKVFDPATLNK
                     LSYDISKAAQGKDTEKYRIYGTATGLMSQNKAGVLPTRNFSEGVFEHAEEVSGEYLDK
                     HHKVKVIACAQCPIACEQMSIVKTGPFAGAMTGIEYESLFANSSNLGISDIRAAIKLI
                     TISDHDGMDSISMGVTIGFAMECFERGVLTEKDFACPSHPEGFTLNFGDGDSAVKLAE
                     MIRDREGIGDLFAEGVRIASKKLDEERGSESWKWAMHTKGLEPGGYDSRSLKTFALGL
                     AVGTRGGCHNRSAAYDPDMKGETNRLTVDETRGRVAAESEEYAAVYDTLPLCKFIRRC
                     FTGKADRSGAWPAIANLINATTGWNYGYDDVDLIGIRAHTIKKSFNIREGWTQADDDL
                     PWRWKNEAMHSGPGAGVYVTEEELAYLKKIYYEAKGWTPEGLIPKELLIKLGMPDVAE
                     EIGV"
     gene            complement(2797..5505)
                     /locus_tag="PKL60_06220"
     CDS             complement(2797..5505)
                     /locus_tag="PKL60_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNW95764.1"
                     /translation="MIPYRVPKNDEAMIVRRGKFLTLVQTAMNKRELQFVRQACLIWL
                     ASYPGDLLVNYIYAAILAELGDVEMAAGNLEKVIAYDPEFLEAMNLLSQLVNGEAIDV
                     EYHSSLAYLQRNPAPVPPVTRWFAPLWNARRAFEAGDQEAAEKAILQALAHNPSLPLP
                     AILHMRIIHKNGNMTLLDTLAGIYGNRWPDCIQIKLLSALADIQQGEDARGVEKLHWS
                     AAHDISGQAAIRLLGADHAFKPIWPDDLKVYFDLPIPASIAVELGWNALATPGANIAP
                     SASGEATVSAQPAASQTAAAGSLPATQKISDFANTNPVDYSEVPPEAFLLSKSPNFGD
                     EKHRKARNETVDSLNEIQAEFDKIAKTMRKSELTAADARFPNYVLMTSRSALTNKYGA
                     NTANVILDAMQDLSIKITALPGWNSVVFVPDDPRIANDLGLTPSLANDAWKFKLALAD
                     LDKSLAAKGEMIGTLLIIGGNDIVPFHQLPNPTDDADNYVPSDNPYATVDDNYFIPQW
                     PVGRIPDEAGSDPVYLIEQLRYLNNEYALKLKAKTLISGTIFENWLINLRESLNATMQ
                     NFRRSEQLGYCAEVWKIPSTEVFNVIDRGDRLKSSPPVEASSFVTTQKSNPKFAYFNL
                     HGLKDSPEWYGQSDLTRRLNSPEYPVALIPESFSSNAPDIVLSEACYGANILDKTAKD
                     ALALKLLAAGSRAFVGSTVIAYGSVNKSLVGADLLAHSFWTHVQNTVPVGYALMRAKL
                     TLASQMTQAQGYLDGEDQKTILSFVLYGDPLASKESLRNIPKPLLRPSKTLVIKTISD
                     SHEEMVVSPEEMPDEILENVKKVISAYLPGLDNATVAINPQLTNFSLDTAGVKDRKAR
                     KHYLEGSERYVVTLRKSVEIKQIPHDHYARMTFDQKGDIIKLSLSK"
     gene            complement(5502..>10019)
                     /locus_tag="PKL60_06225"
     CDS             complement(5502..>10019)
                     /locus_tag="PKL60_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNW95765.1"
                     /translation="EALKAIHNAFEQFSASQAQLLRELAFALENSDPEEARKTWEQVL
                     NLAPQAAVYKEEYAEFLLAHGETEYALDLLNQVPASAQKTLLLLRYPELRAGMELNNE
                     DLRKLPGKTVSLSRFQREPDSLKAARYAFENKNYALADELIGKALLTNPNDLETLKLA
                     GQIKKHTADLDAAIQASELVALYEPDNITTKKELASLFLQSRQPEKALEIYQELISAN
                     PEPSRSDLLTYAEVAIKAEKPEIAIPICESFLSRDTLDGEALVTLCAAHIRKGDEASA
                     ISLLEQTSAIAPEKPASWLALAEIWTSLGRTEMAMESLRKAKAALPANPGILLALGKL
                     YTENERPTEAVGVLKQAFQLDQNDPEIRKSLAKAYLTHGYLNEAWSVIGPLEADYTSD
                     PDLALTLGKVMLALGDYQATKPVLRFAWQSSRSEEALKTYANLLLQAPAHSGGFSAGD
                     TKELNQLMEAVHEKARPESGSFDFKVLEADIQYAQGNMESAYKAYLELLDQPEGKTPR
                     TYFHLQHQIGQCALKSGHPEISLSALQEAVLVNPDNLEVRHTLSEAFAAAGMPEESLA
                     AAKATLQLAPTELDNVLWYSSFCSKNGNEREAVQILKDTLYLLPDEQALYLALARAYL
                     AMGSTAETKETLNRMLEREDISTEEYVNVANIYLHMNDSAEAARIIRLAIANNPQPDF
                     LEARKLAYTVLRLGDGAAALQLLAELEKTLGSHPCFPILKSDVLAGNKQFLPALQNLD
                     SLLKEIEFSGSDLSFEATFGAETDASIPDYNWAGVLYRAAQLERASGDLAAAQKHAGQ
                     AFALQADKSEYILLKSELAFALRKTEKLDSTLELLENNPLSAAHQEMTRLLAVNALIE
                     RDPDKTHLLLDHFLARQSPSAVFHAARAFLALTEGKADRTEEELRSAEALMDEAYARS
                     GLEAFSIPAHFAWVWQALAVATAAFEAQAWDLADKCFGYALAEIKINPVVNQQLAEYL
                     VEKARVSSNARALRIVQHLPHPFSAEKPDEDIHQEQISIAGRYIPAAEMLPTLKIGQA
                     LFSGRWNDGEELKQLVRNSRQAAQALSVQLDSDTIKNICAAFPGDFEVAFQEAVLLLY
                     KNPAACADKAESLLRKHPDKPGLYTMLAIAHQAEPEKAAEAIEKALEIWPDEPDWHAI
                     AGSFHEQCGAFDKAAKHLEDAIHILPKSAQYWQMLGDIKVLEKDYHAARDYFTKAHEL
                     FPGDPEALFSLAVINQQLGEHQAAIQCLREVETLDPGKTVYAESIAESLFALQDYQAA
                     QDQANRVLQTNAHSSRALQVKAKALMKQRQYEEARRVLQAAREMVSDPIPFDLLAIEL
                     DAGMLRKSGLGAALALAESNPENPLVLNTLAGYYMEAAMQTQAEETLHHSLEIDGQNP
                     QTLVMLGRIARRRGNLDQALAYLTRALAVNPSLIEAYLELGQTYQDRRDNTRALETYH
                     RAIEMVEKDPRAYIQAAAAYKDSKDYRNAESMLRQAAQFAPNDPVIRRQLASIVALNL
                     VNNLQEAPKRR"
BASE COUNT         1928 a         2718 c         2921 g         2452 t
ORIGIN      
        1 caggcgccgg ctgtgcttgc tgggggttgt tgggctcggc gctcatttag gttccactgc
       61 tttggtaggg atgtccaacg tctccgcgat gagctttttt cgggcgcgct gcgtacgctg
      121 ctccgcggtg gtcagagtga gcgcttcttt ggggcaccat ttgacgcatt gcgggccttc
      181 cgggtcggtt tcgcactggt cgcaaatttc agccagggct gtggcagggt tgattgaaat
      241 cgcgccgaaa tcacaggctt cgacgcacca ggcacagccg tcgcatttgg tggagtccac
      301 gcgaataatc cctttgtccg ggtcctgagt catcgcgtcg cgcggacata caatgacaca
      361 aggcgcattt tcacaggtgc ggcaggcgat agcgctgatg agcacttcat ccacccggat
      421 cgtgcggatg cggctgcgga agctgttgta ttcgccggtc ttgctgaacg aacacagata
      481 ctcgcacaat tggcagccga cacacaaatt gggatcacat tcgatatagg cgtattttga
      541 tgtactggtt actgacatcg attttctcct tatactccaa tttcctcagc gacatcaggc
      601 atgcccagtt taatcaggag ttcttttgga atcagtcctt ccggggtcca acctttagcc
      661 tcataataga tcttcttcag atacgcgagt tcttcctcgg tgacatacac gcctgcgccg
      721 gggcctgaat gcatggcttc attcttccag cgccatggca ggtcgtcatc agcctgggtc
      781 cagccttcgc ggatgttgaa agattttttg atggtgtggg cgcggatacc aatcagatcc
      841 acgtcatcat acccataatt ccagccggtg gtggcattga tgagattggc aatcgcgggc
      901 caggcgccag atcgatctgc tttgccggta aaacagcgcc ggatgaattt gcacaagggc
      961 agggtatcgt aaacggccgc gtattcttcc gattcagcgg caacccgacc gcgggtttca
     1021 tcgacagtca ggcggttggt ttcgcctttc atgtcagggt cataggctgc ggagcggtta
     1081 tggcagcctc cgcgcgtgcc aactgccagc cccagcgcga aagtcttcag cgaacgggag
     1141 tcgtatcccc ccggttccag gcctttggtg tgcatagccc atttccagct ttcactgcct
     1201 ctttcttcat ccagcttttt ggaggcgatg cgtacgcctt cggcgaataa atcgccgatc
     1261 ccttcgcggt cgcgaatcat ttccgccagc ttgaccgcgg agtcaccgtc cccaaaatta
     1321 agcgtgaaac cttccgggtg agagggacag gcaaaatctt tctcggtcag aacacccctt
     1381 tcaaaacatt ccatcgcgaa accgatggtg acacccatgc tgatagagtc cataccgtca
     1441 tggtcggaga tggtaatcag tttgattgcg gcgcgaatat cggaaatccc cagattggaa
     1501 ctgttcgcga acaggctttc gtactctatg cccgtcatgg cgcccgcgaa agggccggtc
     1561 ttgacgatgg acatctgttc acaggcaatc gggcactggg cgcaggcaat caccttaacc
     1621 ttatgatgct tgtccaggta ttccccgctg acttcctcgg cgtgttcaaa gacgccttca
     1681 gagaagttgc gcgtagggag aacacccgcc ttgttttggc tcatgagccc tgtggcggtg
     1741 ccgtaaatgc gatatttctc cgtgtctttc ccttgcgcgg ccttcgagat atcataggaa
     1801 agtttgttta gtgtcgccgg gtcaaatacc ttaacccctt ttgttccccg cacggaaaaa
     1861 gccttgagtt tcttgtgtcc ccaaatcatt ccatgaccgg tgcggccgag ctgtctgccg
     1921 tcattggtaa cgttggcata ccgaaccagg tgctcaccac aaatcccgat agtacaggaa
     1981 cgtatctgct catctcccat cttctcgcgg atggcttctt ccgtttcgaa ggtgccttta
     2041 ccaagcagat aagtagcatc aaaaaattgc acgcggtcat cgtcgataaa gagatgcgtc
     2101 caattctcgc agctgttgtg cagcactacg ccgtcccagc ctgctcgttt gagcgcgatg
     2161 gagaaccagc tgcctgagag gctgtcgcct ataaaaccgg tcaacgggga tttggaagcc
     2221 ataccgaact tgccaccaac aggaacgggc gtgcctgtga aaatcccatt ggcaaaacaa
     2281 atcgggttgc cgtcgtccaa cgggtcgcaa cccgggacga tattttccca taacagacgg
     2341 gaagccatgg aaacgccgcc aatccagagc ttgtaccact cttccggctt ttcttcgata
     2401 cgggttttat gggttctcag gtcgatgtgc agaatttttc cactgtaacc gtacatttta
     2461 actccctttc gtagccgcat ccatcccacg cggatagaac ggcctcaaac atacttcaca
     2521 cgcaattgta atcaaaaatt ggatatcaaa cgtcaattcg aacaaaccct ttttttgtga
     2581 tttctgaatt tattatacac ttagtcgtaa aatctgtggg gattctcccc tgtttctgaa
     2641 ggaggtaacc tatggttttt tctgaaagtc cggccggcga gagactctgc gcagcgaaag
     2701 aagtcttttg gaaataactg aagcccccgg gtgcgagggg cttcagagcg gaggggaaaa
     2761 ctggatgcta cgccagagtt ttctgcaggg aagagcttat ttcgagaggc tcagtttgat
     2821 gatatcccct ttctggtcga aggtcatgcg ggcgtaatgg tcatgcggga tttgcttgat
     2881 ttccacgctc ttgcgtaatg taaccacgta gcgctcgctg ccttccaggt aatgcttgcg
     2941 ggccttgcgg tctttcacgc cggcagtatc cagcgagaaa ttggtcaact gcgggttaat
     3001 cgccacggtg gcgttatcca ggccgggcag gtacgcgctg atgacctttt tgacattctc
     3061 cagaatttcg tccggcattt cttcagggga aacaaccatt tcctcgtggg aatcgctgat
     3121 ggtcttgatg accagcgtct tggaagggcg cagcagcggt ttgggaatgt tccgcaggct
     3181 ttccttgctt gccagcgggt cgccatagag gacaaaggac aggatggtct tctggtcttc
     3241 accgtcaaga tatccctggg cttgagtcat ctggctggca agcgtcagct tggcgcgcat
     3301 cagcgcgtag ccgacgggaa ctgtattctg cacatgcgtc cagaagctgt gcgccagaag
     3361 gtcggcgccg accagggact tgttgacaga cccataggca attaccgtgc tgccgacgaa
     3421 cgcgcggctg cctgcggcca gcaattttaa cgctaatgcg tctttggctg tcttgtcgag
     3481 gatgttggcg ccgtagcacg cttcgctcag gacgatatcc ggcgcgttac tgctaaagct
     3541 ttcgggaatg agcgcgacag ggtactcagg gctgttgagc cgacgcgtta aatcgctctg
     3601 cccgtaccac tcgggggaat cctttagccc gtgcaggtta aagtacgcga actttggatt
     3661 cgatttctgt gtcgtcacaa agctcgaggc ctcgaccggc gggctggatt tgagcctgtc
     3721 cccacggtca atcacattaa agacttccgt actggggatt ttccacactt ctgcgcagta
     3781 accaagctgt tcagagcgtc tgaagttttg catggtcgcg tttagtgact cgcgcagatt
     3841 gatgagccag ttttcaaata tcgtcccgga aatcaatgtc ttggctttca gtttgagcgc
     3901 gtattcattg ttgaggtaac gcagctgctc aatcagatac accgggtcgc tgccagcttc
     3961 gtccgggatg cgtccgaccg gccactgggg gatgaaataa ttgtcgtcta cggtggcata
     4021 tggattatcc gagggcacat aattatccgc gtcatcggta gggttgggga gctggtggaa
     4081 ggggacgatg tcattcccgc cgataatcag cagggtgcca atcatctcgc ctttagccgc
     4141 caggctttta tccaggtctg ccagcgccag cttgaacttc caggcatcgt ttgccaggct
     4201 gggagtcaga ccgaggtcgt tggcaatgcg ggggtcatcc ggtacaaaca caacactgtt
     4261 ccagccaggg agggcggtaa ttttgattga gaggtcctgc atcgcatcca ggatgacgtt
     4321 ggcagtattg gcgccgtact tgttggtaag ggcagagcgg gaggtcatca ggacataatt
     4381 cgggaagcgc gcgtccgcgg cggtcaattc agacttgcgc atggttttgg cgattttatc
     4441 gaattccgcc tgaatttcgt tcaaggagtc gacggtctca ttccgggctt tcctgtgctt
     4501 ctcgtcgcca aagttcgggg atttgctgag caggaaagct tcgggcggga cttcggagta
     4561 gtcgaccggg ttggtgttcg caaaatccga tatcttttgg gttgccggca agctgccagc
     4621 cgcggcggtc tgtgaagcgg caggctgcgc cgaaacggtg gcttcgcctg aagctgaagg
     4681 cgcaatattt gcgccgggcg tggcgagcgc gttccagcca agttccaccg cgatggatgc
     4741 cgggataggc aggtcgaagt agactttcag gtcatccggc caaattggtt tgaaagcatg
     4801 gtcggcgcca agcaggcgaa tggcagcctg cccgctgata tcgtgggctg cgctccaatg
     4861 cagtttttca acgccgcgcg cgtcctcgcc ctgctggata tccgccagag cggaaagcag
     4921 cttgatttgg atgcagtccg gccagcggtt tccgtagata cctgccagag tatccagcag
     4981 ggtcatgttc ccgtttttgt ggatgatgcg catgtggaga atggcgggaa gcggcaggct
     5041 cgggttgtgc gcgagcgctt gcaggatggc tttttcggct gcttcctggt cgccagcttc
     5101 aaaggcgcgg cgggcgttcc ataacggcgc gaaccatctc gttacgggcg gcacgggcgc
     5161 ggggttgcgc tgcaggtagg ccagcgatga gtggtactct acgtcaatcg cttccccatt
     5221 taccaattgg ctgagcaggt tcattgcctc caggaattcg ggatcgtagg cgatgacttt
     5281 ttcgaggttg cctgcagcca tctccacatc ccccaattcg gccaaaatag cggcatagat
     5341 gtaattcacc agcaggtcgc ccggatagct tgccagccag atcaggcagg cctgacggac
     5401 aaattgcagt tcgcgcttgt tcatcgcggt ctgcacaagc gtcaggaatt tgccgcgcct
     5461 gacaatcatg gcttcgtcat tttttggtac gcgataaggt atcatcttcg ttttggtgcc
     5521 tcctgcaagt tgttcaccaa attcagggca acaatggatg caagctgccg gcggatgacg
     5581 gggtcattcg gggcaaactg cgctgcctgg cgcagcatgc tttcagcgtt gcggtagtct
     5641 ttgctgtctt tgtatgccgc agcagcctgg atgtacgccc gcgggtcttt ctccaccatt
     5701 tcgatggctc tgtggtaagt ctccagggcc cgggtgttat cccggcggtc ctgataggtt
     5761 tgcccaagct ccaggtaggc ttcaatcagg ctggggttca ctgccagcgc gcgggtcagg
     5821 tacgccaggg cctggtccag gttgccgcgc ctgcgcgcga tgcgcccaag catcacaagc
     5881 gtctgcggat tttgcccgtc gatttccagg ctgtggtgca gggtttcttc agcctgagtt
     5941 tgcatagctg cttccatgta atagccggcc agggtattga gcaccagcgg gttctccggg
     6001 ttggattcgg ccagcgccag cgcggcgccg agccccgatt tgcgcagcat gcccgcgtcc
     6061 aattcgatgg caagcaggtc aaagggaatc gggtcgctga ccatttcccg ggcggcttgc
     6121 aagacgcgcc tggcttcttc gtattggcgc tgtttcatca aggcttttgc cttcacctgc
     6181 agcgcgcggc tgctgtgcgc gttggtctgc aaaacccggt ttgcctggtc ttgcgccgcc
     6241 tggtagtctt gcagagcgaa cagggattcg gcgatgcttt cagcatagac ggtttttccg
     6301 gggtccaagg tctcaacctc gcgcaggcat tgaatggcag cctgatgctc accgagctgc
     6361 tggttaatga ccgccagtga gaacagcgct tcggggtccc ccgggaagag ctcatgagcc
     6421 ttggtgaagt aatcccgcgc cgcgtggtaa tctttttcca gaactttgat gtcgcccagc
     6481 atctgccagt actgcgcgga cttgggcagg atgtggatgg catcttcaag gtgtttggct
     6541 gctttgtcaa acgcgccgca ttgctcgtgg aagctgcccg ctatcgcgtg ccagtccggt
     6601 tcatccggcc atatctccag cgctttttca atcgcttcag ccgctttttc aggttccgcc
     6661 tggtgggcta tggccagcat tgtatacagc ccgggcttgt ccgggtgctt cctgagcagg
     6721 ctttcagcct tatccgcgca ggctgccggg tttttataca gcagcaggac agcttcctga
     6781 aaagccacct caaaatcccc cgggaaagcc gcgcagatgt tcttgattgt gtcgctgtcg
     6841 agctgaacag agagagcctg ggcagcctgg cggctgttcc tgacgagctg tttcagctcc
     6901 tcgccgtcgt tccagcgccc gctgaaaagc gcctgaccga ttttgagcgt aggcagcatc
     6961 tctgctgccg gaatgtaccg gccggctatg gaaatctgtt cctggtgaat gtcctcgtct
     7021 ggcttttccg cgctgaacgg gtggggcaga tgctgaacaa ttcgtaaagc gcgcgcgttg
     7081 ctgctgaccc tggctttctc taccaggtat tccgccagct gctggttcac gaccgggtta
     7141 atcttgattt ccgccagcgc gtacccgaag catttatccg ccaggtccca ggcttgggcc
     7201 tcaaatgccg cggttgcgac tgcaagcgcc tgccataccc aggcgaaatg cgccgggatg
     7261 ctgaaagcct ccaaacccga gcgggcataa gcttcgtcca tcagcgcttc agcggagcga
     7321 agctcttcct ccgtccggtc ggctttgcct tcggtaagcg ccaggaaggc ccgcgccgca
     7381 tggaaaactg ctgaggggct ctgccgtgca aggaaatggt ccagaagaag gtgggtttta
     7441 tccgggtcgc gctcaatcag cgcgttcaca gccaaaaggc gggtcatttc ctgatgcgcc
     7501 gcagaaagcg ggttattttc cagcagctca agcgtggaat ccagtttttc tgtttttcgc
     7561 agcgcgaagg ccagctcgga cttcagcagg atgtattcgc ttttgtccgc ttggagcgcg
     7621 aaggcttggc cggcatgttt ctgtgccgcg gccaggtcgc cagaggcgcg ttccagttgg
     7681 gcggcgcggt acaagacgcc cgcccagttg taatccggga tactcgcgtc tgtttcggcg
     7741 ccaaaagtgg cttcaaagga taggtcggag cccgaaaatt cgatttcctt tagcaggctg
     7801 tcaaggttct gcagtgctgg cagaaattgc ttattgcctg ccaggacgtc tgacttgagg
     7861 atcgggaaac aggggtggct gcccaacgtt ttttccagtt ccgccaacag ctgcagcgcg
     7921 gctgcgccgt cccccaggcg gaggactgtg tacgcaagct tgcgggcttc gaggaaatcc
     7981 ggctgaggat tattcgcaat agccaggcga atgatgcggg ccgcctcggc ggagtcgttc
     8041 atgtgtaaat agatattcgc gacgttgaca tactcttcag tgctgatgtc ttcgcgttcc
     8101 agcatgcggt tgagcgtctc ttttgtctcc gcggtggaac ccatcgcgag gtaagctctc
     8161 gccagcgcca gatagagcgc ctgttcatcc ggcaacagat acagcgtgtc tttgaggatc
     8221 tgcaccgctt ccctttcatt tccattctta ctgcagaagc tgctgtacca cagcacatta
     8281 tcgagctccg tcggagcgag ctgcagggtg gctttggcgg ctgccaggct ttcctcgggc
     8341 atccccgcgg cagcaaaggc ttccgagagg gtatggcgca cctccagatt gtcggggttt
     8401 accaggaccg cttcctgaag cgcggaaagg ctgatttccg ggtgccctga cttgagagcg
     8461 cactgcccaa tctggtgctg cagatggaaa taggttcgcg gggttttgcc ttcgggttgg
     8521 tccaacagtt caagataggc tttgtaggca gattccatat taccctgggc gtactgaatg
     8581 tctgcttcga gcactttgaa atcgaaggag ccgctttccg gtctggcttt ttcgtgaacg
     8641 gcttccataa gctggtttaa ctctttcgtg tccccggcgg aaaaacctcc agaatgcgct
     8701 ggggcttgca gcagcaggtt ggcataagtc ttcagcgctt cctccgagcg gctggactgc
     8761 cacgcgaaac gcaagaccgg ctttgtcgcc tggtaatctc ctaatgcgag catcactttg
     8821 ccaagcgtca gcgccaggtc gggatccgag gtgtagtcag cttccaatgg accgatgact
     8881 gaccaggctt cgttcaggta gccgtgcgtc aggtatgcct tggcaagcga tttgcgtatc
     8941 tccgggtcgt tctggtcgag ttgaaaggct tgcttgagga cgccgaccgc ttcggttggc
     9001 cgttcgtttt cggtatagag cttgccaagc gccagcagaa tgcctggatt agcgggtaag
     9061 gcagctttgg ctttccgcaa ggattccatt gccatttccg tcctgcccag gctcgtccag
     9121 atttccgcca gggcgagcca gctggcaggc ttttcaggcg caatcgcgga ggtctgctcc
     9181 aacaggctga ttgcgctggc ttcatcgccc ttgcggatgt gcgcagcgca caatgtcacc
     9241 agcgcttcgc catccagggt gtcgcggctg aggaagctct cacaaatcgg gatggcaatt
     9301 tcgggttttt ccgcttttat tgcgacctcc gcgtaggtca gcaggtcgga gcgggaaggc
     9361 tccggatttg cggaaatcag ctcctggtaa atttccagag ctttttccgg ctggcgggac
     9421 tgcaagaaaa ggctggcaag ctcttttttg gtcgttatgt tatccggttc atagagcgcc
     9481 accaattcag aggcctggat ggccgcgtca aggtctgcgg tgtgtttctt aatttgacct
     9541 gccagtttga gagtttcgag gtcgttcggg ttggtcagca gcgctttgcc aatcagctca
     9601 tcggcaagcg cgtaattctt gttttcgaaa gcgtagcggg cggctttcag gctgtccggt
     9661 tcccgctgaa agcgggaaag cgagaccgtt ttccccggga gcttgcgcag gtcttcgttg
     9721 ttaagctcca tgccggcgcg cagctccggg tatcgtaaga gcagcaaggt cttttgcgcg
     9781 gaggcaggca cttgatttag caaatccagg gcgtattccg tttccccgtg agcgagcaag
     9841 aactcagcgt actcctcctt atagacagcg gcttgcggcg caagattgag gacttgctcc
     9901 caggttttcc gggcttcttc cgggtcactg ttttccaacg caaaggccag ttcacgcaag
     9961 agctgagctt gactggcgct gaactgttcg aaggcgttgt ggatagcctt gagggcttc
//
LOCUS       DAOQAD010000067         9947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_7960_length_9947_cov_6.182471, whole genome shotgun sequence.
ACCESSION   DAOQAD010000067 DAOQAD010000000
VERSION     DAOQAD010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7960_length_9947_cov_6.182471"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="PKL60_06230"
     CDS             <1..1659
                     /locus_tag="PKL60_06230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNW95766.1"
                     /translation="ARPEALAPLLNHLVDDLNALILKSATIDAFLYGLITTNSFDKQA
                     EQMLSRFEIARVPFQNLLVRLRAWFGRLGQALPAALNSVGSARAHAFMLLEEAEQSRY
                     QMSEPEELLANELTLSGGSAWDKLQGTLTSQKTVPFELDGKVQDLPMPALINLRSHPS
                     AEVRQRAYETEQHVWEELKEPLAACLNGVKGETITLDRHRGRRDSLHASLDMARIDQA
                     TLDAMLAAIQASLPSFRRYFQAKARLLGMEKLPWWSLLAPLGESNTAYSFEEARDVIL
                     THFGSFSAELKEFARTAFEQRWIDAEQRPGKRGGAYCMEIPALKESRVLCNFDGSLDQ
                     VMTIAHELGHGFHNYCAFKADKTPLQTRTPMTLAETASIMCETIVFNAILASVKGTQD
                     ELPILESNLSGCAEVIVDIYSRFLFEQEVFTRRQKSTLSADEISEIMLDAQRTSYGNG
                     LDENVLHKYMWTWKPHYYSPSLAFYNYPYAFGLLFATGLYAIFQERGEAFVPEYMQLL
                     ASTGEASAADLAARFGIDIRDQAFWAASLRVIARRIERYIELSL"
     gene            complement(1775..2179)
                     /locus_tag="PKL60_06235"
     CDS             complement(1775..2179)
                     /locus_tag="PKL60_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNW95767.1"
                     /translation="MQTLSVYHLVKSEDLNHHRTLFAGRGAEWLVEAGFIAAANLLPP
                     EFVLCVKIHGMEFRRPVRPGEIVCFTSRVVLTGRSRVIVYIRADVKGEQAVDGFITFV
                     YVDEHGKPRPHGIVIEADTPEEKALQERAKNL"
     gene            complement(2185..4014)
                     /locus_tag="PKL60_06240"
     CDS             complement(2185..4014)
                     /locus_tag="PKL60_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNW95768.1"
                     /translation="MNRFTRFFRVLAALSTAVMLCSACLGAPAQQPAHSASLAAPAQA
                     IEMGRSFDLRLTFANAGGEEALVREIRVPAAILQAFRYLGSLPAVTLERMADGSGVLR
                     TQLVIPPQSSADFVLRFQAIFSGVYEENFSVLFGDELLQLPARLQTRGSIPAGWLPGP
                     ASARGQTAATEGLPEAALVRVKALVNVEGTLVEAWSASGVFISRDGLILTSARAVVGS
                     RAYPVADLIVALQSAPGTAPVDAYLASIVQVDEARDAALIKPRSDLLGNRIPPGSLAL
                     PAAALGESSTVLPGEALTLLGYPSSPDAPAGELSAAVTSLSPEEAGMQLETIMTNLPP
                     GGDYFGWAAFNSRGELVGLASAPDGTSEQACGALADTNRDDIIDEQDACLPAGGNLQT
                     LSSVQALGDMFEEAWMGQVGLRRVSVSGFPFEMQGQVVFRTDFSDPLPAWPAGEQNGA
                     RSAYDEEAFTLTLNAPRSLFFTTLDYAYEDILLRADARVLAGSGDGDFGFICGYRDET
                     HFTALEVSEDAYFSIWKRAGEETSVLVDWTYADALAADGSLRLSAQCGAGALRLAANG
                     LLLAEITDPGFFPGPVGILAGTSAGGTLKIAFDNLEILIPGAE"
     gene            4152..5423
                     /gene="xseA"
                     /locus_tag="PKL60_06245"
     CDS             4152..5423
                     /gene="xseA"
                     /locus_tag="PKL60_06245"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNW95769.1"
                     /translation="MTRTFLLVILTRMDAFDFNAALIFSVTSLNNYLRELLETDEVLQ
                     DLWVRGEISNFSQPRSGHLYFTLKDSEAQIRCVMWKNSALRLRFSPADGMQVEGHGAM
                     SIYPAQGQVQLYLDALRPAGEGALYQEFLRLKARLEAEGLFDPEHKRPLPSLPRHIGV
                     VTSPTGAALQDILQTLSRRLPILRVTIAPTPVQGAEAPPGIVAALRHLNQLEDLDLII
                     LARGGGSIEDLWAFNDESVARAIYNSRVPVISGVGHETDFTIADFVADMRAPTPTGAA
                     ELATPITAQELSLSLRAASDQLDARLNELLSSARQSLALAQAELRRSSPRAFLQNALQ
                     RLDETQARLERLGHNQLEARNLRLASSVSRLNALSPQAVLRRGYAIITDQHSGVLISR
                     ASQTQPGQELLLRLQDGLIPAQVTAKPTGAE"
     gene            5425..5655
                     /gene="xseB"
                     /locus_tag="PKL60_06250"
     CDS             5425..5655
                     /gene="xseB"
                     /locus_tag="PKL60_06250"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNW95770.1"
                     /translation="MPASPENQPSFEAAFADLQAVIDQLENTELPLEEALRLYEEGKR
                     LSMLCSSILESAQLRVSALNETNSTATASERD"
     gene            5657..6124
                     /locus_tag="PKL60_06255"
     CDS             5657..6124
                     /locus_tag="PKL60_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNW95771.1"
                     /translation="MRFWDLFKSPPALAGAFALLLAQLLKVPFGYWRTREINWSLLLD
                     SGGMPSSHSALISSVTSAIGFYVGWGSPLFALAFALSIIVVYDAAGVRRQAGLQAARI
                     NLIVREVIQTGKIPEKEWETLRESIGHSPAEAFAGVLLGFVVAVVIRLIMPPA"
     gene            complement(6219..7889)
                     /gene="murJ"
                     /locus_tag="PKL60_06260"
     CDS             complement(6219..7889)
                     /gene="murJ"
                     /locus_tag="PKL60_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNW95772.1"
                     /translation="MNETPVQEAESSLPGKRVEEKPLKNEAGAPADGAESASRQIARA
                     TGIVMLAFILSSLVGVVQQVVITNAFGTSAALDSFNAANRITELLFNLVAGGALGSAF
                     IPMFTGFLTRGDKRGAWRLASGVVNMVFLVLIAVSILCWIFAPWIVKNGLYALTADSN
                     IGQLDVTVRLLRIMLPTVVVFGISGLVMGMLNAHRSFFIPALAPAIYSLGIIFGTVAL
                     PKSWGVDRLAVGVLLGACGHLLLQLPSLLRLPERSYQISVGMHDKDVRQVLKLMLPRL
                     VGAGVVQLNFVANTIIALSLGEGAASAVALAFMLMLMPQRAIAQSAGIASLPTLSEQA
                     ELGQYQQMRGTIAKLLRGILLLALPASAGLILLRVPLVRVLYERGEFGPESTRLVAWA
                     LLWYSAGLVGHSLVEVLSRAFYALHDTRTPVIIGVGAMSLNVALSFLLTSWFRSIGWM
                     AHGGLALANSAATGLESLLLLALLRKRLKGVEGRHILRGTAAALAGSAVMSLAVYGWL
                     TVVPDSGGLLALLGGMGIGVLVYGLALKLLRVPELDSILALVKRKLRV"
     gene            complement(7903..9381)
                     /gene="mazG"
                     /locus_tag="PKL60_06265"
     CDS             complement(7903..9381)
                     /gene="mazG"
                     /locus_tag="PKL60_06265"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNW95773.1"
                     /translation="MMTPSITIVGLGPAGAELLTREAWDWLSQTDELYLRTSLHPCAA
                     ELPGTLRVHSFDALYEKHEALVDVLDEIVRTVLELGRRAGGVSYAVPGSPWVAEETCA
                     RILEQAKAAGIPVRVINGMSFLEPVCAALGIDPGNQVVMIDALQLAEKNVPTFPPSCG
                     ALIQQLGGGLDASEIKLTLMSAYPDDYPVRLVHAAGTPQQSVEDLPLYALDHSKKLGV
                     ISTLYVPPRENGYSFEDLQEIIARLRAPDGCPWDREQTHLSLRPYLLQETYEVLEALD
                     REEPDELREELGDLLLQILLHAQIATENEDFRMSDVIQAISSKLIRRHPHVFGEVQVH
                     SVDNVLQNWEKIKAKERKDNGNSHKTGMLDGIPLALPALTQADQIQRRAKQVGFDWNS
                     IEPVIAKVREELEELNEAETSAERRAEAGDLLFAAVNLLRWLDIDPETALRECNLRFR
                     KRFAYIEAEAAKAGKSVDGLSFEEMDALWEDAKKAFAARNED"
     gene            complement(9445..9699)
                     /locus_tag="PKL60_06270"
     CDS             complement(9445..9699)
                     /locus_tag="PKL60_06270"
                     /inference="COORDINATES: protein motif:HMM:NF025156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HNW95774.1"
                     /translation="MDKWEYRTVFVSYAQIRGSKGLFKMEKDWVVNYSDGSQTVGFEK
                     ILNKYGELGWELVNVVVEHSLGEGSASTDQYRLFFKKRII"
BASE COUNT         2047 a         3303 c         2712 g         1885 t
ORIGIN      
        1 gcccgcccgg aagccctggc tccgctcctc aatcacctgg tggacgacct gaacgcgctc
       61 atcctgaaaa gcgccaccat cgacgccttc ctctacggcc tcatcaccac aaactccttc
      121 gacaaacaag ccgagcagat gctttcgcgc ttcgaaatcg cgcgtgtgcc cttccaaaac
      181 ctgctggtgc gcttgcgcgc ctggttcggc cggctcggtc aagccctccc cgccgccctg
      241 aacagcgttg gcagcgcccg cgcacacgcc tttatgctgc tggaagaagc cgaacaaagc
      301 cgctatcaaa tgtcggagcc tgaagagctg ctcgccaacg agttaaccct ttcgggcggc
      361 agcgcctggg ataaactgca aggcacgctc acctcccaaa aaaccgtgcc cttcgagctg
      421 gacggcaaag tgcaagacct gccgatgccc gcgctcatca acctgcgcag ccacccttcc
      481 gccgaagtgc gccagcgcgc ctacgagacg gaacaacacg tctgggaaga acttaaagaa
      541 cccctggctg cctgtctgaa cggcgtcaag ggcgaaacca tcacgctgga caggcaccgc
      601 ggccggcgcg acagcctgca tgcctccctg gatatggcac gcattgacca ggcaacgctg
      661 gatgcgatgc tggccgccat tcaggcctcc ctccccagct tccgccgcta cttccaggcc
      721 aaagcccgcc tgttgggcat ggaaaagctg ccctggtgga gcctgctggc cccgctgggc
      781 gagagcaata ccgcgtacag cttcgaagaa gcccgggatg tcatcctgac ccacttcgga
      841 agcttttcag cggaactcaa ggaattcgcc cgcactgctt tcgaacagcg ctggattgac
      901 gcggagcagc ggcccggcaa acgcggcggc gcttactgta tggaaatccc cgccctcaag
      961 gaaagccgcg tgctgtgcaa ttttgacggc tctctggacc aggtcatgac gatcgcgcac
     1021 gaactgggcc acggcttcca caactactgc gccttcaaag ccgacaaaac cccgctgcaa
     1081 acgcgcaccc cgatgaccct ggcggaaact gcctccatca tgtgtgaaac tattgttttc
     1141 aacgcgattc tggccagcgt aaagggtacg caggacgagc tgcccatcct ggagagcaat
     1201 ctttccggct gcgccgaggt gattgtggat atctattcac gcttcctctt cgagcaggaa
     1261 gtcttcaccc gccggcagaa atccacactc tctgctgatg aaatctccga aattatgctg
     1321 gacgcgcagc gcacaagcta cgggaacggc ctggacgaaa atgtcctgca caaatatatg
     1381 tggacctgga aaccgcacta ctacagccct tccctggcat tctacaatta cccctacgcc
     1441 tttggcctgc tctttgccac cggcctgtat gccattttcc aggagcgcgg cgaggcgttc
     1501 gtgccggaat acatgcagct gctcgccagc accggcgaag cttccgctgc ggacctggct
     1561 gcccgctttg gcatcgacat ccgcgaccag gccttctggg ctgcttcctt gcgcgtcatc
     1621 gcccgccgca tcgaacgcta catcgagctc agcctctaac ttctgccaaa gcaaagccgc
     1681 ggttggcttc ccaccgcggc ttttttgttg cctcagctgc agaactggca gacggcctga
     1741 agtcggcgca gcgcccgctg agcgcctgcc ggcattacag gttcttcgcg cgctcctgca
     1801 gcgctttctc ctccggggta tccgcttcaa tcacaatccc gtgcgggcgc ggtttgccgt
     1861 gctcatccac gtacacaaag gtaatgaagc cgtccaccgc ctgctcgcct ttgacatccg
     1921 cgcggatata cacaatcacc cggctgcgtc cggtcagcac tacgcggctg gtaaagcaca
     1981 caatctcccc cgggcggaca ggtcttctga actccatccc gtggattttg acgcacagca
     2041 caaactcagg gggcagcagg ttggccgccg cgataaagcc ggcttccacc agccattctg
     2101 cgccgcgccc ggcaaaaagc gtgcggtggt ggttcaggtc ttcgctcttg accaaatggt
     2161 agacactcaa agtctgcatg cccattattc agctcctgga ataagaattt caagattatc
     2221 aaacgcaatt ttcaatgttc ctccggcaga agtgccagca agaattccga ccggacctgg
     2281 gaaaaaccca gggtccgtaa tctcggccag cagcagaccg ttcgctgcca gcctcaatgc
     2341 tccggcgccg cactgcgcgc tcaggcgcag gcttccgtcg gcagccaggg catccgcgta
     2401 ggtccaatcg accaacacgc tggtctcctc acctgcccgc ttccagatag aaaagtaagc
     2461 gtcctcgctg acttccagcg cggtgaagtg cgtctcgtcc cggtatccgc agataaagcc
     2521 gaaatcgccg tctccgctgc cagccaacac gcgcgcatca gcgcggagaa ggatgtcttc
     2581 gtaagcgtaa tcgagcgttg taaaaaacag actgcggggc gcattcaaag tgagcgtgaa
     2641 agcctcctca tcataagcgc ttcgcgcgcc gttctgttct cccgcgggcc aggctggcag
     2701 agggtccgaa aaatccgtcc gaaatactac ctgcccctgc atctcaaaag ggaaaccgct
     2761 aaccgaaacg cgccgcagcc ccacctgccc catccaggct tcctcaaaca tatcccccag
     2821 cgcctgcacg gagctgaggg tctgaaggtt cccgcccgcg ggcaggcagg cgtcctgctc
     2881 atcaatgatg tcatcgcggt tggtatccgc cagcgcgccg caggcctgtt cactggtccc
     2941 atccggcgcg gaagccagcc ctaccagctc gccgcgcgag ttaaaagcag cccaaccaaa
     3001 ataatctccg cccggcggca gattggtcat gatggtttca agctgcatgc ccgcctcttc
     3061 aggggagagg ctcgtcacgg cggcgctcag ctcacccgcc ggcgcatccg ggctggaagg
     3121 atatcccagc agcgtgagcg cctcacccgg cagcacggtc gagctttccc ccagcgcagc
     3181 cgcggggagc gcaagcgaac cgggcggaat cctgttcccc aacaggtctg aacgcggttt
     3241 gataagcgct gcatccctgg cctcatccac ctgcacaatg ctggccaaat acgcgtccac
     3301 tggcgcggta cccggcgcgc tttgcaaggc aacaatcagg tccgcgaccg gataagcgcg
     3361 gctgcccact accgcccgcg cgctggtcag gatgagcccg tcccggctga tgaacactcc
     3421 gctggcagac caggcctcca ccagggtgcc ctccacattc accaacgcct tcacgcgtac
     3481 cagggctgct tcgggaagcc cctctgtcgc agccgtttgc ccgcgcgcgg aggcaggtcc
     3541 gggcagccag ccggcgggga tgctgcccct ggtttgcaag cgcgctggca gctgcagaag
     3601 ttcatcgcca aacaaaaccg aaaaattctc ttcatacacg ccggagaaaa tcgcctgaaa
     3661 gcgcagtaca aagtccgcgc tgctttgagg tgggatgacc agctgcgtcc gcagcacgcc
     3721 cgagccgtcc gccatgcgtt ccaaggtaac cgccggcagg ctgcccaggt acctgaaagc
     3781 ctgcaggatg gccgccggta cgcggatttc gcgcaccagc gcctcctctc caccagcgtt
     3841 cgcgaacgtc aggcgcaggt caaagctgcg tcccatttcg atagcctgtg ccggggcagc
     3901 aagcgacgcg ctgtgcgcgg gctgctgcgc aggcgcgccc aggcaggcag agcacagcat
     3961 gactgccgtg ctgagcgccg cgaggacgcg aaaaaagcgg gtgaatcgat tcaaaatagc
     4021 ggctcctggc tggcacgcgt gccaacctgg gcataattat aacgccgcag ccccagccct
     4081 tcactgccgg cgttcccgtc cttgcagcca ggcttttatc agataaagcc ggccaaaata
     4141 tggtttgcga attgacaagg accttccttt tggttatcct tacccgtatg gacgcgttcg
     4201 atttcaacgc agcattgatt ttttccgtta cctccctcaa taactacctg cgcgaactcc
     4261 tggaaacaga tgaagttttg caggacctgt gggtgcgcgg cgagatttcc aacttttccc
     4321 agccgcgttc cggtcacctc tacttcacgc tcaaagacag cgaagcgcaa attcgctgcg
     4381 tgatgtggaa aaattccgct ctgcgcctgc gtttttcgcc cgccgacggg atgcaggtcg
     4441 aaggtcacgg cgccatgagc atctatcccg ctcaggggca ggtgcagctc tacctggacg
     4501 cgctccgacc ggccggcgaa ggcgcgctct atcaggagtt tttgcgcctg aaagcccgcc
     4561 tggaagcgga aggcttgttc gaccctgagc ataagcgccc tctgcccagc ctcccgcgcc
     4621 atatcggcgt ggtcacctcc cccacaggcg ccgccctgca ggacattctg cagactcttt
     4681 ccaggcgtct gcccatcctg cgtgtcacca tcgcccctac tccggtgcaa ggcgcggaag
     4741 ccccacccgg aatcgttgcc gcgctgaggc atctcaacca acttgaggac ctggacctta
     4801 tcattctggc ccgcggcggc ggctccatcg aggacctgtg ggcgttcaac gacgaaagcg
     4861 tcgcgcgcgc catctataat tcacgcgtac cggtcatctc cggtgtcggt cacgaaactg
     4921 atttcacaat cgcggatttc gtcgcggaca tgcgcgcccc gacgcccacc ggcgcagccg
     4981 agcttgccac gcccatcacc gcccaggaac tctccctgag cctgcgtgcc gcttccgacc
     5041 agctcgacgc gcgcctgaac gagcttctaa gcagcgcccg acagtccctg gctttagccc
     5101 aggcagaact gcgtcggagt tctccgcgcg ccttcctcca aaacgcgctg cagcgcctgg
     5161 acgaaacgca ggcacggctt gaaaggctcg gacacaatca attggaagcc cgaaaccttc
     5221 gcctggcaag ttccgtcagc cgactgaacg cactcagccc ccaggccgtt ttacgcagag
     5281 gctacgccat catcaccgac cagcacagcg gcgtgctcat cagccgcgca tcccagaccc
     5341 agcccgggca agagcttctc ctgcgcctgc aggacggcct tattccggcg caggtgaccg
     5401 cgaaacccac aggagcagaa tagtatgcca gcaagccctg aaaaccaacc cagcttcgaa
     5461 gccgccttcg cggacctgca ggccgtcatt gaccagctcg agaacaccga actgccgctc
     5521 gaggaagccc tgcgtctgta cgaagaaggc aaacgcctct caatgttatg ctcgtctatc
     5581 ctcgagagcg cgcagctgcg cgtctccgcc ttgaacgaaa ccaattccac agcgaccgcc
     5641 agcgaaagag actgaaatgc gcttctggga tttgttcaaa agcccgccgg cattggcagg
     5701 cgcgttcgcg ctgctcctgg cgcagctact caaggtgccc ttcggttatt ggcgcaccag
     5761 ggaaatcaac tggtcgctgc ttttggattc aggcggcatg ccctcctcgc actccgctct
     5821 gatttcttcg gttacttccg ccatcggctt ctacgtcggc tggggcagcc cgcttttcgc
     5881 gctggcattt gccttgagca tcatcgtggt gtacgacgcg gcgggcgtgc gccggcaggc
     5941 cggcttgcaa gccgcccgca tcaacctgat tgtgcgtgag gtcattcaga ccggtaaaat
     6001 ccccgaaaaa gaatgggaaa ccctgcgcga gagcatcggg cactcgcctg cggaagcttt
     6061 tgcgggggtg ttgctgggct ttgtggtcgc ggttgtcatc cggctcatca tgccgcccgc
     6121 gtaaatttcg aattcgcttc ctgccatccc cgacaaaaat accgggtaaa cctcacccgg
     6181 caggaatcgc aagcctctcc aacggcgcca ggctgcgttt acacgcgcag tttgcgcttc
     6241 accagcgcga ggatggaatc caactccggc acgcgcagca gcttaagagc gagcccgtac
     6301 accagcacgc caattcccat cccgcccagc agcgcaagca agccgccgga atcgggcact
     6361 accgtcaacc agccgtagac ggccagactc atcactgcgg aacctgccag tgcggctgct
     6421 gtgccacgca aaatatgccg gccttccacg cccttcaatc ttttccgcag taacgccaac
     6481 aaaagcaggc tttccaaacc ggtcgccgcg gaattagcca gcgccagccc gccgtgcgcc
     6541 atccacccga tgctgcggaa ccaggatgtc agcaagaaag acagcgcgac attcaggctc
     6601 atcgcgccca cgccgataat aacgggcgtg cgggtgtcgt gcagcgcgta aaaagcacga
     6661 ctgagcactt ccaccagact gtgcccgacc aaacctgctg aataccacag cagcgcccag
     6721 gccaccaggc gggtggactc ggggccgaac tctccgcgct cgtacaacac gcgcaccagc
     6781 gggacccgca gcaaaatcag gcccgcgctg gccggcagcg ccagcaaaag tatgccgcgc
     6841 agcagcttcg cgatggttcc ccgcatctgt tggtactgcc ccagctccgc ctgctcggag
     6901 agcgtgggca acgaagcaat ccccgccgac tgcgcgatgg cgcgctgcgg catcagcatc
     6961 agcatgaacg ccagcgcgac ggcgctcgcg gcgccttcac ccagactgag ggcgatgatg
     7021 gtattcgcta cgaaattgag ctgcaccacc cccgccccaa ccaggcgcgg cagcatcagc
     7081 ttcaagacct gccggacatc tttgtcgtgc atcccaaccg agatctggta gcttcgttcg
     7141 ggcagccgca agagcgaagg cagctgcagc agcagatgcc cgcaggcgcc caagagcacc
     7201 cccacagcca gtctgtccac accccagctc tttggaagcg cgacggtgcc aaaaatgatg
     7261 cccagcgaat aaatcgccgg cgccagagcg ggaataaaga aggaacggtg ggcgttcaac
     7321 atacccatca ccagcccgct gatcccaaaa actaccacgg tcgggagcat gatgcgcagc
     7381 aagcggaccg tgacgtccaa ttgaccgata ttcgaatccg cggtcagggc gtacaaaccg
     7441 ttcttcacaa tccacggggc aaaaatccag cagagaatcg agacggcgat gagcaccagg
     7501 aagaccatat ttaccacgcc ggatgccaga cgccaggctc cgcgtttatc tccgcgcgtg
     7561 aggaaaccgg tgaacatcgg gatgaatgcc gaacccagcg cgccgcccgc caccaggtta
     7621 aacagcagtt cggtaatgcg attcgcggca ttaaaagaat ccagcgccgc gctggtgcca
     7681 aaagcattgg ttatcacaac ctgctgtact acccctacca ggctgctgag aatgaacgcg
     7741 agcatgacga tgccggtggc gcgtgcgatt tgtcggctgg cgctttccgc cccatccgcc
     7801 ggcgcgcccg cctcattctt caggggtttt tcttccaccc gttttcccgg cagcgaagat
     7861 tccgcttcct gtaccggggt ttcgttcatg ccgctgttca tctcagtctt cgttccgggc
     7921 ggcaaaagcc tttttcgcgt cttcccagag cgcgtccatc tcttcgaagc tgaggccatc
     7981 cacgcttttg ccggctttgg cggcctccgc ttcgatatac gcgaaacgct tgcggaagcg
     8041 gaggttgcat tcgcgcagag ccgtttccgg gtcgatatcc agccagcgga gaaggttcac
     8101 cgccgcgaaa agcaaatccc cggcttcagc ccggcgttcg gcgctagtct cagcttcgtt
     8161 cagctcttcc aactcctcgc gcaccttggc aatcactggt tcgatgctgt tccagtcaaa
     8221 accaacctgt ttggcgcgcc gttgaatctg gtctgcctgg gtcagcgccg gcagcgccag
     8281 cggaatgccg tccagcatgc cggttttgtg gctgttgccg ttatctttgc gctccttggc
     8341 tttgattttt tcccaattct gcagcacgtt atcgaccgaa tggacctgca cctccccaaa
     8401 cacatgtgga tgccggcgaa tcaacttgga gctgatagcc tgaattacat cgctcatccg
     8461 gaagtcttca ttttccgtcg cgatttgggc gtgcagcagg atttgcagaa gcaaatcgcc
     8521 cagctcctcg cgcagttcgt ccggctcctc gcggtccagc gcctcgagta cttcataagt
     8581 ctcctgcagc aggtacggac gcagagagag atgggtctgt tcgcggtccc aggggcagcc
     8641 gtcgggcgcg cgtaagcgcg cgatgatttc ctgcaggtct tcaaaggaat acccgttttc
     8701 gcgcggcggc acgtaaaggg tggaaatgac gcccagtttc ttggaatggt caagcgcata
     8761 aagcggcagg tcttccacgc tttgctgggg cgtgccagcg gcatgcacca gccggacggg
     8821 gtaatcatcc gggtacgcgc tcatcaacgt cagcttgatt tcgctcgcgt ccagcccgcc
     8881 gcccagttgt tggatgagcg cgccgcagga gggcgggaag gtgggcacgt ttttctccgc
     8941 cagctgcagg gcgtcaatca tcaccacctg gttgcccggg tcgatgccca gcgccgcgca
     9001 caccggctca aggaagctca tcccattgat gacgcgcacg ggaatccctg ccgctttagc
     9061 ctgctccagg atacgcgcgc aggtttcttc cgccacccag gggctgccgg ggacggcgta
     9121 gctcacgccg cctgcgcgcc tgcccagttc cagcacagtc cgcacgattt cgtccaaaac
     9181 gtccaccagg gcttcgtgct tctcgtagag cgcgtcaaag ctgtgtaccc tcagcgttcc
     9241 gggcaattcc gcggcgcagg ggtgcaggct ggtgcgcagg taaagttcgt ccgtctgcga
     9301 gagccagtcc caggcttcgc gggtcagcag ttccgcgcct gccgggccga gcccgacgat
     9361 agtaatggat ggggtcatca tgaagaaacc atttccggat gctgtagctt atcctactgt
     9421 gcttagttgt ttttgtatca tgtattaaat aatacgtttt ttgaagaaaa gacgatattg
     9481 atccgtggaa gcagacccct cacctaagga gtgctcaaca acgacattta ccagttccca
     9541 gcccagttca ccgtatttgt tcaatatttt ctcaaaaccc acggtttggc tgccatcgga
     9601 atagttcaca acccaatcct tttccatttt gaaaagccct ttggaacccc tgatctgcgc
     9661 atacgaaaca aaaacagttc ggtactccca tttatccatt ctggcaaacc ctcccaaagc
     9721 tctgcgtctc acaaactaga aaaggctgcc cattattaac ggacagcctc ttgattgtaa
     9781 cgcacttcag tttccgcgaa cacgaaaact atagatcttc tcagcaagtc aaacgtgtgg
     9841 gagacccaaa agtcgctccc acggtcgaat cctggtgatg attaacgctt ggtgtaggtc
     9901 ggggccttgc gggcgcgctt gagacctggc ttcttgcgtt ccttaac
//
LOCUS       DAOQAD010000068         9870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_8045_length_9870_cov_5.598574, whole genome shotgun sequence.
ACCESSION   DAOQAD010000068 DAOQAD010000000
VERSION     DAOQAD010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8045_length_9870_cov_5.598574"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..683)
                     /locus_tag="PKL60_06275"
     CDS             complement(144..683)
                     /locus_tag="PKL60_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95775.1"
                     /translation="MSKGIGKVVAGIITVVIGGTAFAISKTDVIKNFSEETGMTQQEA
                     QQYIEAISEEELISFDQLGEDHITDGQDALNLASEIDCVKYQYEWESDTLSCEEGLAQ
                     YIEFGESSIALGESYKVLGSDSASNADIEAAIRQIDRFNECLRSEIIVTLMGQANADE
                     VIKSNLYNKALLQTALESN"
     gene            959..1882
                     /locus_tag="PKL60_06280"
     CDS             959..1882
                     /locus_tag="PKL60_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNW95776.1"
                     /translation="MELPGRILRILPVILLLPLLAACASELKTPPPQLPLTLPTATPL
                     PQPTVHFSANTPPPESPPGAEPSPTPTQLTISYTHGWMVSDQLSGEPLEETMPFWVYL
                     PPGYGQNPDRRYPTLYLLHGLNFDESQWKNLGLAAEMDALIAAEKIPPFIVILPRVPN
                     ISTYPSNYNARIFADLLIPYVDGRYQTLPERGCRAIGGLSRGAAWALRIGMQDGELFS
                     RIGLHSLSMSGEEINTWVSELKALLPQERPELYMDAGSSDEDQDSAQFLSYQLNRESI
                     PHRFILVEGEHSTDYWSEHLAEYLLWYSSGW"
     gene            2014..2400
                     /locus_tag="PKL60_06285"
     CDS             2014..2400
                     /locus_tag="PKL60_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNW95777.1"
                     /translation="MYQKLILIGNLGRDPEMRFTADGKPVTSFSMATSRKYKEKEETT
                     WFRITVFGSQAESCNQYLKKGSKVLVEGRLNPDTNGSPKVYQRKDGGWGASYEVTAQS
                     VRFLSSNRSEDVGNEEDVPGDEDVPF"
     gene            2409..2726
                     /locus_tag="PKL60_06290"
     CDS             2409..2726
                     /locus_tag="PKL60_06290"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNW95778.1"
                     /translation="MPEMKPISLRLPADLKPLFANLVRAAHTPGEFILDFSAVLPGQE
                     FSQVDARIILSPLGAKLLQQVLNENIHRYESNFGEIRLPNPPHSLADDLFSQAKPNPP
                     EGG"
     gene            2728..3369
                     /locus_tag="PKL60_06295"
     CDS             2728..3369
                     /locus_tag="PKL60_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HNW95779.1"
                     /translation="MSELDSISDQIRLEFDRKSARRDAALAQTRQLTRHASLAIRAIH
                     REDEAEAERELQSARELARALRAGLTDDPDLYFAGYTQDALKEFVEAELTVALILNHS
                     LPTPADLNAEYPAYLAGLAETLGELRRRCLDLLRPGYSSEVERLLMWMDDIFTQLVTM
                     DYPDAITEGLRRRTDLARGIIERTRADITLSFRQSELEQALTRLSEQLNDETD"
     gene            3369..3596
                     /locus_tag="PKL60_06300"
     CDS             3369..3596
                     /locus_tag="PKL60_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95780.1"
                     /translation="MRVLRASELGSYLYCARAWWYQRQGVPSENQAELKTGTNFHQQH
                     ARSVLLSFLLRAAALFLFLLACALLILKAAR"
     gene            3593..4117
                     /locus_tag="PKL60_06305"
     CDS             3593..4117
                     /locus_tag="PKL60_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HNW95781.1"
                     /translation="MRDWLWIILLLLASALLFLWSARKHRQTGLPTGKIVYADTDAWQ
                     ALPAPLFDPTLRLTGKPDYAVDIGSGVRVPVEVKSAPAPIAPYDSHVMQLAAYCLLLE
                     RETGRRPPYGLLRYRDKTFQVEYTPALEESLLSLIASIRAAEKCPDGPDRSHAQAARC
                     RACGYRQHCTQALG"
     gene            4268..4367
                     /gene="ffs"
                     /locus_tag="PKL60_06310"
     ncRNA           4268..4367
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKL60_06310"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4383..5288
                     /locus_tag="PKL60_06315"
     CDS             4383..5288
                     /locus_tag="PKL60_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNW95782.1"
                     /translation="MNTKSTETPPVCSYEGSDYQRTFWDSGERAYEDAVEQIALRRLL
                     VKPGKLLLELGAGAGRNTPRYTGFERVVLLDYSRTQLLQARQRLGADPRYLFVAADIY
                     KLPFVDGLFDGATMIRTLHHMAQPGLALASVRRVLAENARFLLEFANKRNLKAVLRYL
                     SGRQEWNPFSPEQVEFAELNFDFHPKTIRRLLKENRFSIEKQLNVSQFRVAWLKRHVP
                     LKALVALDSALQWTGAFAQYAPSIWTLAASTGASPRPAQGSFFTCPVCGSALSEVGQS
                     QSCPQCAHYWSYADGIHEFRINPEA"
     gene            5260..6873
                     /locus_tag="PKL60_06320"
     CDS             5260..6873
                     /locus_tag="PKL60_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95783.1"
                     /translation="MSSGLTRKPESPPPARRSLLLLLAGLGFLSGCNPPSSALNPATA
                     TLISARQTEAAGLYPSPTPLFGSSPAPYQTPAFQTTPEPLPPQAPVFTPTAAPDMPIP
                     EGMRLYYSQSGDTLAAVASHFLARPWELQQPYALDEQELLPVGTPILVPDNFAVEPPY
                     AQPALPDADILFSQGSGSLDLEGWIEQGAGYLAGYHQTISGERMSAAQIIQLVAVENS
                     MNPRLLLALVEYRSGWLFSFPQELEQYQYPIGYYAPSVSGLFDELTVTARLLGQGYYG
                     WRLGTLDHLTFRDDSQAPIHPALNAGSAGIQNLFAALYTPDLFWDSLYGPQGFLALYT
                     RLFGDPWARAALQGPLMPAGISQPALSLPFLPGLTWSLTSGPHIAWQAGTPRGALDLA
                     PRTGQRGCYLSSTWASASAKGLVVRSQRGALALDLDGDGDEGTGWVLVYLHLAGEERV
                     PLGSWVEADAPLGHPSCEGGVSTGTHLHFARKYNGEWIPAEGPLPLILDGWQAYAGAS
                     GYGGWLIRDGETVLSSATRDPISEIRRDP"
     gene            complement(6875..8182)
                     /locus_tag="PKL60_06325"
     CDS             complement(6875..8182)
                     /locus_tag="PKL60_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW95784.1"
                     /translation="MSVSKTFAGGRVRSALRTYLARVKAFSPNARKYLLGIMLYGAAI
                     GIFQLLFNFYVLSLGYNEAVLGNLVTARSATTLIVALPVGYLADRIGKKLAFVIGQGA
                     VGAAILLMILFPSLPMFLLMNIVIGFAQSLAAVAMGPFLMENSGEVERTYLFSFSSGL
                     RMTAVSIGEWVGGFLPTWIGKDLGALPTSAQAYSGALALVTIGVAFSLIPVFLIRQQE
                     RTRSDRASFAPLSYMRRNPGKLTRLILPLLLTSIGAGMIMPFMNVFFRNVHQLSDASI
                     GVLFAWGSLAMGIGLLLAPALAERFGKIEVVVATQGLSIPFLALLGFSPLFPVAAAAY
                     YLRLVLMNMSGPVYTTFTMEHVEPESRAMVASLSTMASNFGWAFSPTLSGYFQVNYGF
                     GPSFGVTILFYIVSVVMYYLWFGKKSKPAVVAEGAPATVEIRQ"
     gene            complement(8184..9242)
                     /locus_tag="PKL60_06330"
     CDS             complement(8184..9242)
                     /locus_tag="PKL60_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNW95785.1"
                     /translation="MPAPTFSKLHWDLGTGYDLFISQIAVYEPDRFGLRASWAAGVRS
                     RLTAEDRQTLDNFWQINFLPMPFIFSAPAPKDTAAVLRTLEELPEGKRLEALTLHHET
                     SAEVKEIFCGTTPRRKWTPAEKETISADYARHGLNLTPTLLDLVYSVWAQREQFGQQY
                     LHALKAYAESFFFEEEQRILPALRQGLSHAQMRAGTLPLIPMLEELSSGVRWAEITAA
                     QKLYLAPSFWGAPFLFTEELNNKTLLVVFGSRPDDMALIPGDIVPDNLLRGLKALAEP
                     TRLRILRTLAHSPQTAAHLSRILRLRPPTVNHHLMELRMAGMVHVIISSEGERFYATR
                     FEGFESTQDLLKRFVSEE"
     gene            9455..>9870
                     /locus_tag="PKL60_06335"
     CDS             9455..>9870
                     /locus_tag="PKL60_06335"
                     /inference="COORDINATES: protein motif:HMM:NF022635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HNW95786.1"
                     /translation="MPNILDYVDWRGDLEFQRDPLNEVDNLVFSVLSYMDFAGAVPTT
                     EEAGSISLADATEAIKQMPEQVTAPITSAFFSRLPALLEKCAGCARYREARLSHYINK
                     VSYDEAEQFSALVFSVDEKTHFVAFSGTDDTLIGWK"
BASE COUNT         2052 a         3138 c         2742 g         1938 t
ORIGIN      
        1 ttttcgtcag cttgagcgcg gaggtctgcc tgcggatgtt gggtttcgct cccgctccgc
       61 tgcacccggt ctccatcctg ggaggccgtc agtcataacc gccggttcgg tcaaaaaacc
      121 agcggaggaa agcgctctcc gccttaattg ctttccagcg cagtttggag taacgctttg
      181 ttatacaaat tggatttgat cacctcatcg gcgtttgcct gacccatcaa tgtcacaata
      241 atctcggaac gcaggcattc attaaacctg tcaatttgcc tgatcgctgc ttcaatatcc
      301 gcattggaag ctgaatctga gcctaacacc ttatagcttt ccccaagcgc gatgctgctt
      361 tctccaaact cgatatattg tgccaaccct tcctcacagg acagcgtgtc agattcccat
      421 tcatattggt acttaacgca atcaatttca gaagctaagt tgagagcatc ttgtccgtca
      481 gtgatatgat cctcgcccag ctggtcgaaa gaaatcagct cctcctcgga tatggcttca
      541 atatactgtt gagcctcttg ttgggtcatg ccagtttctt ctgaaaagtt tttaatgaca
      601 tcagttttgc taatcgcaaa ggcggttcca ccaataacga ctgtgatgat ccccgcaaca
      661 acctttccta ttccctttga catttaccct cccacgatat caatttatgg tacatcgaaa
      721 aaatcccaaa cgtaaatata tcacgagaaa aacaggatga cagaaattca tcgcgaattc
      781 cccttttaaa tattccccca cagaatgaac attgacaaca gtcatagacc gggctgcgcc
      841 ctcaccccga cgctttcggc ggctcgaaaa gcgcaagccg cccctacaac cgccagatcg
      901 gtcagggcac cgacaggagc aagcaccctg cccgtgcctc aggagtataa tccctcgcat
      961 ggaactcccc gggcggattc tccgcattct cccagttatc ctcctgctcc ccctgctcgc
     1021 cgcctgcgca tccgaactca aaaccccgcc gccccagctt cccctcaccc tgccgaccgc
     1081 caccccactg ccacaaccga cggttcattt ctccgccaac acgccgcccc ccgaatcccc
     1141 gcccggcgcc gaaccatccc ccacacccac ccagctcaca atctcctaca cgcacggctg
     1201 gatggtctca gaccagcttt ccggcgagcc gctcgaggaa accatgccgt tctgggtcta
     1261 cctgccgccg ggctatgggc agaaccccga tcggcgctac cctacgcttt acctgctgca
     1321 cggcttgaac ttcgatgaga gccaatggaa gaacctcggc cttgccgcgg agatggacgc
     1381 gctgattgcc gcggaaaaaa tcccgccttt catcgtcatc ctgccgcgcg tcccgaacat
     1441 ctccacctac ccctccaact ataacgcgcg cattttcgcc gatttgctga tcccctacgt
     1501 ggacgggcgc taccagaccc tgccggaacg cggctgccgc gcgattggcg ggctatcccg
     1561 cggggctgcc tgggcgctgc gcattggcat gcaagatggt gagctcttca gccgcattgg
     1621 cttgcacagc ctgtccatga gcggtgagga aataaacacc tgggtgagcg agctcaaagc
     1681 gctccttccg caggaacgcc ccgagctcta tatggacgcc ggcagcagcg acgaagacca
     1741 ggattccgcg cagttcctca gctaccagct caaccgcgag agcattccgc accgcttcat
     1801 cctggtggag ggcgagcaca gcacggatta ctggtcggag catttggccg aatacctgct
     1861 ctggtacagc agcggctggt gaggcccgtt tgtgtacatt cagggatttc atccagaatc
     1921 tgcagtaaaa ttgaagcaac aaggaagcaa tttgttcctg ataactgaag actcaaacct
     1981 tacacctgaa cgcgaacaag aaaggaacac cccatgtacc aaaagttaat cctgataggc
     2041 aacctcggca gagaccctga aatgcgcttc accgcggatg gcaaacccgt tacctccttc
     2101 agcatggcta ccagccgcaa atataaagaa aaagaggaaa ccacctggtt ccgcatcacc
     2161 gtattcggct ctcaggcaga atcctgcaat caatacctga aaaaaggttc caaggtcttg
     2221 gtggaaggcc gtctgaaccc agacaccaac ggttccccca aggtttacca gaggaaagac
     2281 ggcggctggg gtgccagcta cgaagtgact gcccaaagcg tgcgcttcct cagctccaac
     2341 cgcagcgaag acgtcggtaa tgaagaggat gtccccgggg atgaggacgt cccattctag
     2401 gagttccgat gcccgaaatg aaacctatca gcctgcgcct gccggctgac ctgaagccgc
     2461 tgtttgccaa cctggtgcgt gccgcgcaca cccccggcga gttcatcctg gatttctccg
     2521 cggtgctgcc cggccaggaa ttcagccagg tggacgcgcg catcatcctc tcccctttgg
     2581 gcgccaaact gctccagcag gtcctgaacg aaaatatcca ccgctacgag agcaacttcg
     2641 gcgaaatccg gcttcctaac ccgccgcatt ccctggcaga cgacctgttc agccaggcca
     2701 aacccaaccc accggaaggc ggctgagatg agcgagctgg atagcatttc tgaccagatt
     2761 cgactggagt tcgaccggaa gtccgcccga cgggatgccg ccctggctca gacgcgccaa
     2821 ctgacccgcc atgcctcgct ggctatccgc gccatccacc gcgaagacga agccgaagct
     2881 gaacgcgagc tgcaaagcgc gcgcgaactg gcgcgtgcgc tgcgcgcggg ccttactgac
     2941 gacccggacc tgtactttgc gggctatacg caggacgcgc tcaaggagtt cgtcgaagcc
     3001 gaactgaccg tcgcgctcat cctgaaccac agcctgccta ctccggcgga cctcaacgcg
     3061 gaatacccgg cctacctggc cggtctggcg gaaacgctgg gcgaactgcg ccggcgctgt
     3121 ctggacctgc tgcgcccggg ctactccagc gaggtggaac gcctgctgat gtggatggat
     3181 gacatcttca cccagctggt caccatggat tatccggacg cgattaccga aggtttgcgc
     3241 cgccgtactg accttgcccg cggcattatc gaacgcaccc gcgcggatat taccctcagc
     3301 ttcagacaga gcgagctgga acaggcgctc acccggctct ctgaacagct gaatgacgaa
     3361 acagactgat gcgcgtcctg cgcgcttcgg aactgggcag ttatctttac tgcgcccgcg
     3421 cctggtggta ccagcgccag ggcgtgccgt ctgaaaacca ggcggaactt aaaaccggaa
     3481 cgaattttca tcagcagcac gcccgcagcg tgctgctgtc tttcctcctg agggctgcgg
     3541 cgctgtttct tttccttctg gcttgcgccc tgctcatcct gaaggcggcg cggtgagaga
     3601 ctggctgtgg atcattctgc tcttgctcgc gtccgcgctg ctgtttttgt ggtccgcgcg
     3661 caaacaccgc caaaccggtc tgcccaccgg caaaattgtc tacgcggaca cggacgcctg
     3721 gcaagccctg cccgccccgc tctttgaccc caccctgcgc ctgaccggca aacccgatta
     3781 tgccgtggat atcggcagcg gcgtgcgcgt ccctgtggaa gtgaaaagcg cgccggctcc
     3841 gatcgcgccc tacgacagcc acgtgatgca gctggcggcc tactgcctgc tgttggaacg
     3901 cgaaaccggc cgccgcccgc cttacggatt gctgcgttac cgcgacaaaa ccttccaggt
     3961 ggaatacacc cctgccctgg aagaaagcct gttgagcctc atcgccagca tccgcgcggc
     4021 ggaaaaatgc ccggacggtc ccgaccgttc gcacgcgcag gccgcgcgct gccgggcgtg
     4081 cggataccgc cagcactgca cgcaagcgct gggctgagcc ccaaaagccc ccgccagcca
     4141 tttcgctcat cattattagt ttgtccactt tctgtcaccc ctatggtgac attttaactt
     4201 tggcgtaact ctgctcccct gctaagttga cagccttgct tgtctcggtt atgcttattt
     4261 ggcgttcggg cgggaccggc gcggcggagc cctgcgaacc ccgtcaggtc cgagaggaag
     4321 cagcggtaag gaggtcgctt cgggtgttgc cggcaaacct gcccgaccgc ttttcattca
     4381 ccatgaacac gaaatccacc gaaaccccac ccgtttgttc ctacgaaggc tctgattatc
     4441 agcgcacatt ttgggatagc ggcgaacgcg cctatgagga cgcggtcgaa caaatcgcgc
     4501 tcaggcgcct gctcgtcaaa ccgggcaagc tgctgctgga gttgggcgcc ggcgcggggc
     4561 gcaacacccc gcgctacacc ggcttcgaac gcgtcgtcct gctggattac tcgcgcaccc
     4621 agctgctgca ggcgcgccag cgcctgggcg cagacccgcg ttacctgttc gttgccgccg
     4681 atatctacaa gctgcccttt gtggacggtc tgttcgacgg cgccacgatg atccgcacgc
     4741 tgcaccacat ggcacagccc ggcctggcgc tcgcgagcgt ccggcgcgtg ttggctgaaa
     4801 acgcccggtt cctgctggaa tttgccaaca aacgcaacct caaggccgtc ctgcgctatc
     4861 tcagcggcag gcaggaatgg aacccctttt cgcctgagca ggttgaattt gctgagctta
     4921 acttcgactt tcaccccaaa acgatccgcc gcctgctgaa agaaaaccgc ttcagcatcg
     4981 aaaaacagct gaatgtttcc cagtttcggg tagcctggct caagcggcac gtcccactca
     5041 aggccctggt agcgctggat tctgccttgc agtggaccgg cgccttcgcg caatacgccc
     5101 ccagcatctg gacgcttgct gcaagcacag gcgcttcacc gcgccccgcg caaggcagct
     5161 tttttacctg ccctgtttgc ggaagcgcac tgtctgaagt tgggcagtcc caaagctgcc
     5221 cgcagtgcgc tcactattgg tcttacgcgg acggcattca tgagttccgg attaacccgg
     5281 aagcctgaga gccccccgcc cgcgcgccgg agcctgctgc ttttgttggc agggctcgga
     5341 ttcctcagcg gctgcaaccc gccttcttct gcgcttaacc ccgccaccgc cactctgatt
     5401 tcagcacgcc agactgaagc ggcaggcctt tatcccagtc caaccccgct tttcgggtca
     5461 tcccccgcgc cttaccagac gccagccttc caaacgacgc ccgagcccct tccgccgcaa
     5521 gcgcctgtgt ttaccccgac cgcagctccg gacatgccca tccccgaggg catgcgcctg
     5581 tactactccc agtccgggga tactttggcc gcggtggcca gccatttcct cgcgcgccct
     5641 tgggaacttc agcagcccta cgcgctggat gaacaggagc tgctgccggt gggcacgccc
     5701 atcctcgtcc cggataattt cgcggtagag cctccttacg cgcagcccgc cctgccggat
     5761 gccgacatcc tgttttccca gggcagcggc agcctggacc tggaaggctg gattgaacag
     5821 ggcgcgggat atctggcggg ctaccaccaa accatctccg gggagcgcat gtccgccgcg
     5881 cagattatcc agctggtagc agtcgaaaat tcgatgaacc cgcgcctgct gctggcgctg
     5941 gtggaatacc gttcgggctg gctgttttcc ttcccgcagg agcttgagca atatcaatac
     6001 ccgattgggt attacgcccc cagtgtctcg ggcttgttcg acgagctgac ggtgacggcg
     6061 cgcctgcttg gtcagggcta ttacggctgg cgcctgggca cgctggacca tctgactttc
     6121 cgggacgaca gccaggcgcc catccacccg gctttgaatg ccggcagcgc gggcatccaa
     6181 aacctgttcg ccgccctcta tacccccgac cttttctggg attcgctcta cggtccgcaa
     6241 ggatttttgg cgctctacac ccgcctcttc ggggacccct gggcgcgcgc ggccttgcag
     6301 ggccctttaa tgccagccgg catcagccag cccgcgctca gcctgccatt cttgcccggg
     6361 ctcacctgga gcctcacctc agggccgcat atcgcctggc aggccggcac cccgcgcggt
     6421 gcgttggatc ttgccccgcg tacaggccag cgcggctgct atctctcctc cacctgggca
     6481 agcgcttctg ccaagggtct ggtggtgcgc agccagcgcg gcgcgctggc gttggacctg
     6541 gacggagacg gcgatgaggg caccggctgg gtgctggtct acctgcacct ggccggggag
     6601 gaaagggtgc cgctcggcag ctgggtggaa gccgatgcgc cgcttgggca cccatcctgc
     6661 gagggcggcg tttcgacagg cacgcacctg cattttgccc gcaaatacaa cggcgaatgg
     6721 attccggcgg aaggccccct gccgcttatc ctggacggct ggcaggctta tgccggcgca
     6781 tccggctacg gcggttggtt gattcgggac ggcgaaacag tgctctccag cgcgacgcgg
     6841 gaccccattt ctgaaatccg gcgtgatccg taaattattg ccgaatttcc acggttgctg
     6901 gcgcgccttc cgccaccacc gccggcttac tttttttccc aaaccagagg taatacatca
     6961 cgaccgaaac gatataaaac aaaatagtca ccccgaagga cggaccaaag ccgtagttca
     7021 cctggaaata gccgctgagc gtggggctga aagcccagcc gaaattgctc gccatggtgg
     7081 aaaggctggc caccatcgcg cgcgattcag gctccacatg ctccatcgta aaggtggtgt
     7141 agaccggacc gctcatgttc attagcacca ggcgcaaata atatgctgcc gccgccacag
     7201 ggaagagcgg cgaaaacccc agcagcgcca ggaacggaat ggacaggcct tgcgttgcca
     7261 ccaccacctc aatcttcccg aagcgctccg ccaaagccgg cgccaggagc aaaccgatgc
     7321 ccatcgccag ggagccccag gcaaacaaaa cgccgatgga tgcgtctgaa agctggtgta
     7381 cgttgcggaa gaacacgttc atgaaaggca tgatcatgcc cgccccgatg gaggtcagta
     7441 gcaaaggcag aatcaggcgg gtgagcttgc ctggatttcg gcgcatatac gagagcggag
     7501 cgaaagacgc gcggtcggag cgcgttcgtt cttgctgacg gataagaaag accggaatga
     7561 gtgaaaatgc caccccgatt gtgaccagcg ccaacgcgcc gctgtaagct tgcgcgctgg
     7621 ttggcagagc gcccaggtcc tttccaatcc atgtaggcaa aaaaccgccc acccactccc
     7681 caatcgaaac ggctgtcatg cgcaggccgg aactgaaact gaacaagtag gtacgttcca
     7741 cttcgccgct gttttccatc aggaaaggcc ccatcgcgac ggctgccagc gactgggcga
     7801 agccaatcac aatgttcatc agcaggaaca tgggcagcga ggggaataga atcatcagca
     7861 ggatagccgc gcctaccgcg ccttgcccta tcacaaaagc aagcttcttc ccgatgcggt
     7921 cagccagata cccgactggc aaagccacaa tcaaggtggt cgcgctgcgg gcggtcacaa
     7981 ggttcccaag caccgcctcg ttgtagccca ggctgagcac atagaaattg aacaggagct
     8041 gaaaaatccc gatagcagcc ccatagagca tgatgccaag caggtatttg cgcgcgttcg
     8101 ggctgaacgc ttttacccgc gcgagataag tgcgcaacgc ggagcggacc cgcccgccgg
     8161 caaaggtttt ggatacgctc atgctattct tcgctgacaa agcgtttcag caagtcctga
     8221 gtgctttcaa agccctcaaa acgggtggcg taaaagcgct cgccttcgga agagatgatg
     8281 acgtgcacca tgccagccat gcgcagttcc atcaggtggt ggttcaccgt gggcggacgc
     8341 agccgcaaaa tgcgcgaaag gtgcgcagcc gtttgggggc tgtgcgcgag cgtgcgcagg
     8401 atgcgcagcc gggtaggctc cgccagcgct tttagcccgc gcagcaggtt atccggcacg
     8461 atatcccccg ggattaaggc catatcgtcg ggtctggagc caaacacgac caggagggtt
     8521 ttattgttca gctcttcagt gaacaggaac ggcgcgcccc agaaggaggg cgcgaggtag
     8581 agcttttgcg cggcggtaat ctcagcccaa cgcacgccgg aggaaagctc ttccagcatc
     8641 ggaatcagcg ggagggtgcc ggcgcgcatt tgagcatgcg aaagcccctg ccgcagggcg
     8701 ggcagaatgc gctgttcttc ctcaaagaag aaggactccg cgtaagcctt cagcgcgtgc
     8761 aggtattgct gcccgaattg ctcgcgctgc gcccaaacgc tgtaaaccag gtccaagagg
     8821 gtgggtgtca ggttgaggcc gtgtctggcg taatccgcgc tgatggtttc cttttctgct
     8881 ggggtccatt tcctgcgggg ggtcgttccg cagaagattt cttttacctc cgcagaggtt
     8941 tcatggtgca gggtcagcgc ttccaggcgc ttcccctccg gcagttcctc cagggtacgc
     9001 agcaccgcgg ctgtatcctt tggagccggc gcggaaaaga tgaaaggcat tggcaagaaa
     9061 ttaatctgcc agaagttatc aagggtctgg cggtcttcgg cagtgagcct ggagcgaacg
     9121 ccggctgccc aggaggcgcg cagcccgaag cggtccggct cgtagacagc gatctggctg
     9181 ataaacaggt catagcctgt gcccaggtcc caatgcagtt tggaaaaagt tggtgctggc
     9241 atcgtaacgt ttcctgaata gataattatc tcaattatta gccaaatgtc taataagtca
     9301 agagtcggtt ttccgcaagc gtgtcaagag tcggttttcc gcaagcgggc ctgccctgaa
     9361 cctcttgagg aaagaagcaa gcttaccgca aggcggtttt ttgaggacag cgaaaccaaa
     9421 atcatgtaca atttaagtgc gaacgggagc gagaatgcca aatattctgg actatgtgga
     9481 ctggcgcggg gatctggagt tccagaggga cccgctgaac gaggtggaca acctggtttt
     9541 ttcggtgctg tcctatatgg attttgccgg cgcggtgcca accaccgaag aagccggctc
     9601 gatttcgttg gcggacgcga ccgaagcaat caagcagatg cccgaacaag tcaccgctcc
     9661 catcaccagc gcgttctttt cgcgcctgcc tgcccttttg gagaaatgtg ccggctgcgc
     9721 gcgctaccgg gaagcgcgcc tgtcccacta cattaacaag gtttcctacg acgaggctga
     9781 acaattttcc gcgctggttt tttccgtgga tgagaaaacg catttcgtcg cgttcagcgg
     9841 caccgatgac accctgattg gctggaagga
//
LOCUS       DAOQAD010000069         9425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_8582_length_9425_cov_5.632337, whole genome shotgun sequence.
ACCESSION   DAOQAD010000069 DAOQAD010000000
VERSION     DAOQAD010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8582_length_9425_cov_5.632337"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PKL60_06340"
     CDS             complement(<1..497)
                     /locus_tag="PKL60_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95787.1"
                     /translation="MQTDGEVFQAIISDLEGNAVLLPGNLRARMLSWREIGGCERAEL
                     VLENAPGGYWQGSELIGKSVRVYNRSGSLVWWGYVQSAARNDGEYRLSRSLADLANRV
                     SVRYSELQPQGESFGVKKQTEWALDARSAAHYGQKDLLLERGLMAESAALQLRDQTLA
                     RLAEPA"
     gene            complement(505..1842)
                     /locus_tag="PKL60_06345"
     CDS             complement(505..1842)
                     /locus_tag="PKL60_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95788.1"
                     /translation="MSRLDLGNLADPEESAWYCINEGPMDPGLLGTQYDPTAGPGATR
                     TETLRVGLRGGPLQLRAVIRRLEQLVELVRLKSEQGLGNAIYLRAYPPEGGSPVYSQL
                     LHAELRTLPGALAYEEGGAMAVDLVITRLNWFDGAEQALTLANSGGSGYSASLLNLDD
                     SLAAGNDNYFFVDTSGLQTDLPAPIRLQITNTGSAALGQLFVGAFHERASTEKPVMVL
                     EGEAAPEATGVSASAASGGAFGRFNISGGNWQTFASWELDTITLALLGGRLFLPLLRF
                     QSPLSLDGARFRALLSTNAPVFCSPAAIAPGGARFLALSPLQLPFGSLSPRYAPAGLQ
                     LSLEAFLPAVGAQTLELDDLLLLPQDNFAWFEAAAGIGADERLMDDSFTRESYTVLDG
                     QQLRGHQRVGDWFGLPAGARSWFYCFQVDANGAALPQSSVGVRAWYRTRRQIL"
     gene            complement(1839..2279)
                     /locus_tag="PKL60_06350"
     CDS             complement(1839..2279)
                     /locus_tag="PKL60_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95789.1"
                     /translation="MPTLSELCLHLNQQFLDPQNLNIPADTQTEAVREALAGMNAFLG
                     RQYTLAGLDGALESSLPEDCVPVLVCGAAAFALDFSLRKRLSGFASALGREDTLRLWC
                     AHLSRQFDAGLDRLRALSLQSEAGLPFGAWNWNESPHWRQESEL"
     gene            complement(2266..2469)
                     /locus_tag="PKL60_06355"
     CDS             complement(2266..2469)
                     /locus_tag="PKL60_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95790.1"
                     /translation="MDRLDIRMVAEALAGPDLLGCAVKENGVLVVVDSNGKKRTFYPM
                     DYQHLLHPAGQPTPAKGARHANA"
     gene            complement(2547..2741)
                     /locus_tag="PKL60_06360"
     CDS             complement(2547..2741)
                     /locus_tag="PKL60_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95791.1"
                     /translation="MPKWKVLLSSRKFWAALMGLVVMVVKAWKPDFPLEAGELAGILS
                     VLVAYILGTALEDGLAGHKE"
     gene            complement(2772..4754)
                     /locus_tag="PKL60_06365"
     CDS             complement(2772..4754)
                     /locus_tag="PKL60_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95792.1"
                     /translation="MPEITDPKTAEPAEKPWEQRQRLALEAAPITPEKFEILAITAGS
                     ANGWEFPPEVLRESLQLWEGVNCFVDHDWTSRSVRDIAGVLRKPAWDQEAQGIRAELH
                     AFGPSAQLLARVGREVLEIKDAPAVRVGFSADVLFNGRGKRVEKILRIFSVDLVYNPA
                     RGGMFLRAMNSLGLKPALKGDLLMQTELRENIPAAELSETQQETGPDLQTQLAQLRAE
                     REQMSAKLLDASLAVSSLPAPMTERIRQQFQDRSFAPAELQAAIREARALLSELDRGK
                     TVQGPARIEGMLDPAERLQAAVDDLFGAPRAKALENASVPRLSGIRELYLTLTGDYEL
                     HGGYYPQRAQLAGTSDFSGLVKNALNKLVANTWDELGRAGYDWWKQVTVQEHFSSLHD
                     ITGTLIGTVGDLPAVAEGGNYTELAIGDSPETASFTKYGGYIPLTLELIDRDETRKLR
                     SYARELATAGMRKISKLVAAIFSSNGGVGPTMADTGALFNVTAVTTAGGHANLGTSAL
                     SANAWDAACRAVYKQPMLIKNSAALRGTGPALAINPKFILIPRALQKTAMELCTGALV
                     RESGYVYDNVLKGSAVPVVVPDWSDDSDWAAVCDPRVVPAIFVGERFGLAPEIFVAGD
                     ELSPSVFSNDEHRLKVRHYLAVWVNDFRPLYKSNVA"
     gene            complement(4831..6129)
                     /locus_tag="PKL60_06370"
     CDS             complement(4831..6129)
                     /locus_tag="PKL60_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95793.1"
                     /translation="MGIFRRTNRTSTIEAQDQQADAGELRAGLSLVENDNSFLVGASA
                     LGDQPRDRHAWDRLQVMDDCLDAWRFNPLARRIIELTTQYVTGGGMRVNCAESRTGEF
                     LLAFWRHPLNRMDARLVELSDELARSGNLFLLLSTDPGGMSYLRVVPSADIERITSQP
                     NDVEQELTFHTRPDLNDISLCYPAYDPLQDSPEMPPVMLHYAVNRPAGAQWGESDLAP
                     VLRWLSRYASWLEDRVRLNRFRNAFMYVVKARFATENARAARQSQLAANPPASGSVLV
                     TDESEEWSVIAPRLEALDASTDGLAIKKMIAAGVGLPLHFLAEPESSTRTTAEASDGP
                     TLRRFEQRQRTLRWILEDVLRAALRRAARADSSLNPEAVFQVQAGDISARDNGELAEA
                     GSKAAVVAQQLYAQGLIDKAELLRLVYRFLGEGGEDVKMP"
     gene            complement(6116..6928)
                     /locus_tag="PKL60_06375"
     CDS             complement(6116..6928)
                     /locus_tag="PKL60_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNW95794.1"
                     /translation="MDDCNVNGQFAFGIDLSVYNSSADGKRRPDFERIAAHQPRVEFA
                     ALRTGQSWGYRDPAFAFYVQEARRIGLCVLPYHVVFPAESAARQMDAFLGILEGEDLE
                     HLRLVLDMELEHGQTRRRITDTLNACLEILDEETGRYPIVYSRASWVDQYLEVSDLPE
                     LDWWLAQYYARRAYPGFTPEYPCPPRLPKGISSWLIHQTAERAPAIGGSGWYMDYNRW
                     AGKSEDLLRYFGRQIETVPQLCPLDGKPCPRGTAQNQITHKLKRKEDVYGNL"
     gene            complement(6928..8580)
                     /locus_tag="PKL60_06380"
     CDS             complement(6928..8580)
                     /locus_tag="PKL60_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95795.1"
                     /translation="MDSALSPGGLIHSPESLPRNKTQPAGAVLSGAYANPEPQSRAAF
                     LRSLRSLGVYSRVLGGVRLRAYQDAAGETIARSVLEKLGLSIVVMFPRQSGKNMLQAQ
                     LEVYLLTRFALCGAEMVKFSPTYQPQSLNAMRRLETALQANWLTRGSWKKSAGNHYRF
                     GNAHLTFLSAAPESNVVGATASTLLQLDEAQDIGIEKYDKQIAPMAASTNATRVFWGT
                     AWTTHTLLARELRLARQAEARDGIRRVFCLGAEAVRAEVPAYGLFVDEQVARLGRTHP
                     MVRSQYFGEEIDAEIGLFTPARTGLMQGSQAVQDAPEPGKTYALLVDLAGEDEQLRAN
                     PAGEGDIALSNPGRDSTAITIVVVDIRARAGDLTGKPVYRVARRELWTGEKHSTQYAR
                     LLALVDRWQPRKVVVDASGVGAGVASFLADRLGERVVQLNFNRQVKSRLGWGFLAVVD
                     TGRFQDYLPADPHSQQGQLHALFYRQLQAVAYEVSLGPERNIRWGVPEAARDPMDGSK
                     LHDDLVLSAAMTSVLDEQSWGEAHEPLLIQAADPLREMDGAF"
     gene            complement(8676..9152)
                     /locus_tag="PKL60_06385"
     CDS             complement(8676..9152)
                     /locus_tag="PKL60_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95796.1"
                     /translation="MNHKNPRQTTHPSDSGPACSSGAGRLPVGSEADEQIRALLGDAR
                     AQDADFYLDHFSPGERRELAAAARKTLDAEVVMLRTAMKRFFQAVRSDENPETVERLG
                     DALNLLGLSCTRLGNVLKINQALQGAAKQAGKDDLLEKLMDELALVSAEEEQKDAV"
BASE COUNT         1772 a         3133 c         2713 g         1807 t
ORIGIN      
        1 gctggttcgg ccaggcgggc aagcgtctgg tcgcgcagct gcagcgcggc gctttccgcc
       61 atcaagccgc gctccaggag caaatccttc tgcccatagt gggcagcgct gcgcgcgtcc
      121 agagcccact ccgtttgttt cttgacgcca aaactctccc cctgcggttg gagttcggaa
      181 taacgcaccg agacgcggtt ggccagatcc gccagcgagc gcgagagccg atactcccca
      241 tcattgcgcg ctgcgctctg gacgtagccc caccacacca ggctgccgga gcggttgtac
      301 acgcgcaccg actttccaat cagctcggat ccctgccagt agccgcccgg tgcgttttcc
      361 aaaaccagtt ccgcgcgttc gcagccgcca atctcccgcc aggaaagcat ccgcgcccgc
      421 aaattgcccg gcagcagcac agcattccct tccaaatctg aaatgattgc ctgaaacact
      481 tcgccgtcag tttgcatccg cacctcacag aatctgcctg cgggtgcggt accaggcccg
      541 caccccaacg gaagactgcg gcaaagctgc cccattcgcg tccacctgaa agcagtaaaa
      601 ccaggaacgc gcgccggcag gcagcccaaa ccaatctcca acgcgttgat gcccgcgcag
      661 ctgctgccca tccaagaccg tgtagctttc acgtgtaaag gaatcatcca tcagccgctc
      721 gtctgcgcca atccccgcgg ccgcctcaaa ccaggcaaaa ttatcctgag gcagcaggag
      781 caggtcgtcg agctccagcg tttgtgctcc cacagccggt aaaaacgcct ccagactcag
      841 ctgcagcccg gcaggcgcgt agcgcgggct gagactgccg aagggcagct gcagcggcga
      901 aagcgccaaa aagcgcgccc cgcctggcgc gattgcagcc gggctgcaga acaccggagc
      961 gttcgtgctg agcagcgccc gaaaacgcgc cccgtccagg ctcaacggcg actgaaagcg
     1021 cagaaggggc aaaaaaagcc gcccgcccag aagcgccagg gttatggtat cgagctccca
     1081 gctggcgaaa gtctgccagt tgccgccgga gatgttgaaa cgcccgaagg ccccacccga
     1141 ggccgcggac gcgctgacac cagtagcttc cggcgcggct tccccttcca acaccatgac
     1201 cggcttttcg gtgcttgccc gctcgtgaaa cgctcccaca aaaagctgcc cgagcgctgc
     1261 gctgccggta ttggtgattt gcaggcggat gggtgcgggc aggtcagttt gcagcccgct
     1321 cgtgtccaca aaaaagtagt tgtcgttgcc ggctgccagg ctgtcgtcca ggttcagcag
     1381 gctggcgctg tagccgctcc cgccgctgtt tgccagggta agcgcctgct ccgcgccatc
     1441 gaaccagttc agccgggtga taaccaaatc caccgccatt gccccgccct cttcatacgc
     1501 caacgcgccc ggaagagtgc gcagctcggc atgcagcagc tgggaataga ccggactgcc
     1561 gccctcaggc gggtaagcgc gcagataaat ggcgttcccc aagccttgct cgcttttcaa
     1621 ccgcacaagt tccaccagct gctccaacct ccggatgact gccctcagct gcaaggggcc
     1681 gccgcgcagg ccgacccgca gcgtttcggt tcgggtcgcg cccgggccgg ctgtcgggtc
     1741 gtactgcgtc ccaagcagac cggggtccat cgggccctca ttgatgcaat accaggcgct
     1801 ttcctccggg tccgccaggt tgcccaaatc cagccggctc atagttcgct ctcctggcgc
     1861 caatgcgggc tctcattcca gttccaggcg ccaaaaggca ggccggcctc actttgcagg
     1921 ctcagcgcgc gcaagcggtc cagcccggca tcaaactggc gtgaaaggtg cgcgcaccac
     1981 aagcgcagcg tatcctcgcg cccgagcgcg ctggcaaaac cgctcaggcg cttgcgcagg
     2041 ctgaaatcca gcgcgaaggc agccgccccg cacaccaaaa ccggtacaca gtcctccgga
     2101 aggctgcttt ccagcgcgcc gtccaagccc gccagggtgt actgcctgcc caaaaaggca
     2161 ttcatgcccg ccagagcctc gcgcacagct tcggtttgcg tgtccgcagg gatgttcagg
     2221 ttctgggggt ccaaaaactg ctggttcaag tgcaggcaca gctcgctaag cgttggcatg
     2281 cctggctccc ttcgccgggg tcggctgtcc cgcaggatgc agcaggtgct ggtaatccat
     2341 cgggtaaaaa gtgcgttttt ttccattgct gtccaccacc accagcacgc cgttttcctt
     2401 caccgcgcag ccaagcaggt cgggccctgc cagggcttcc gccaccattc tgatgtccaa
     2461 cctatccatt tttccattct cctcacaagt gtccattttc ccgcgggtgg gcgggcgccc
     2521 ggaaaccagg cgcccgcttg taagcgctac tccttatgcc cggccaggcc gtcttccagg
     2581 gcggtgccca gaatataggc taccagcacg gaaaggatgc ccgccaactc tcccgcctcc
     2641 aacgggaaat ccggcttcca ggctttcacc accatcacga ccaaacccat cagggctgcc
     2701 caaaacttcc gtgagctcaa caggactttc catttcggca tctttttctc cttttttctg
     2761 ctgaaatttt cttacgcgac gttcgactta tacagcggtc taaagtcgtt cacccacacg
     2821 gccaggtagt ggcgcacttt caggcggtgt tcatcattgc tgaaaaccga aggcgaaagc
     2881 tcgtcgcccg ccacgaaaat ctcgggcgcc aacccaaagc gctcccccac gaaaatcgcc
     2941 ggcaccacgc gcgggtcgca caccgccgcc caatcgctgt catccgacca atccggcacg
     3001 accaccggca ccgcgctgcc cttcagcaca ttgtcgtaca cgtagccgct ttcccgcacc
     3061 aaagcgccag tgcacagctc catggcagtc ttctgcagag cgcgcgggat gaggatgaac
     3121 ttggggttga ttgccagcgc cggacccgtg ccgcgcaaag cggcgctgtt cttgatcagc
     3181 atcggctgtt tgtacaccgc gcggcaagcc gcatcccagg cgttcgcgct cagggcgctg
     3241 gtgcccaggt tggcatgacc gcccgccgtg gtgacggctg tcacgttgaa cagcgcgccg
     3301 gtatccgcca tagtcgggcc gacgccgccg ttgctgctga aaatcgccgc gacgagcttg
     3361 gaaatcttgc gcatgccggc agtcgccagt tcgcgcgcat agctgcgcag tttgcgggtc
     3421 tcgtcgcggt caatcagctc caatgtcaac ggaatgtacc cgccgtattt ggtgaagctg
     3481 gcagtttccg gcgagtcccc aatggccagt tcggtgtaat tgccgccttc tgcgacggcg
     3541 ggcaaatccc caaccgtacc aatcagcgtg ccggtgatgt catgcaggct ggaaaagtgt
     3601 tcctgcacgg ttacctgctt ccaccagtcg taacctgccc gcccgagctc gtcccaggta
     3661 ttcgcaacca gcttgttcag cgcgttcttc accaggccgc tgaaatctga agtgcccgcc
     3721 aactgagcgc gctgcgggta ataacctccg tgcagctcgt agtctccagt cagggtcagg
     3781 taaagttcgc gaatgccgct caggcgcggg accgaggcgt tttccagggc tttcgcgcgc
     3841 ggggcgccaa agaggtcgtc cacggcagct tgcaagcgtt cggcggggtc caacatgcct
     3901 tcgatacgcg cgggtccctg gacggttttg ccgcggtcca gttcgcttag cagagcgcgc
     3961 gcctcgcgga tggctgcctg taattccgcc ggcgcgaagc tgcggtcctg aaactgctgg
     4021 cgaatgcgtt cggtcatcgg cgcaggcagg ctgctgaccg ccaggctggc gtccagcagt
     4081 ttcgcgctca tctgctcacg ctccgcgcgc agctgcgcca gctgagtttg caggtcgggt
     4141 ccggtttcct gctgggtttc gctcaattca gccgcgggta tgttttctct caattcggtt
     4201 tgcattaaca aatctccttt caaagccggt tttaagccga gtgaattcat cgcgcgcagg
     4261 aacattcccc cacgcgcggg gttataaaca aggtccacgg aaaaaatcct gaggattttc
     4321 tcaacacgtt ttccgcgccc gttgaacaac acgtccgccg agaaacctac acgaaccgcg
     4381 ggggcgtcct tgatttccag cacctcacgc cccacgcgcg ccagcagctg ggcggaaggg
     4441 ccgaaagcgt gcagttcagc gcggatgccc tgcgcttcct ggtcccaggc gggtttgcgc
     4501 aaaacgccgg cgatatcccg caccgaacgc gaggtccagt cgtggtccac aaagcagttc
     4561 acgccttccc acagctgcaa agactcgcgc agtacctccg gtgggaactc ccagccgtta
     4621 gcgctgccgg cggtaatcgc caggatttcg aacttttccg gggtgatggg cgcggcttcc
     4681 aaagccagcc tttggcgctg ctcccaaggc ttttcagcgg gttcagccgt ttttgggtca
     4741 gtaatttcag gcaatttttt cctttctcca gatatcaggc ttagtttttt gccgcgaatt
     4801 gccgtaccat gcgcgcggct gccgatgcat ttacggcatt tttacatctt cccctccttc
     4861 tccaagaaaa cgatagacca ggcgcagcag ctccgcttta tcgatgagac cctgggcgta
     4921 aagttgctgc gccaccaccg cggctttact tccggcttcc gccagctctc cgttatctcg
     4981 cgcggagata tcgccggcct gtacctgaaa aaccgcctcg gggttcaggc tcgagtccgc
     5041 tcgggccgcc cgccgcagcg ctgcccgcag cacgtcctcc aaaatccaac gcaaagtgcg
     5101 ctgcctttgt tcaaagcgcc gcagagttgg tccgtcagaa gcttccgcgg tcgtgcgcgt
     5161 gctggattcc ggctcagcca gaaagtgcag cggcagcccc acgcccgcgg caatcatctt
     5221 cttgatggcc aagccatccg tggaggcgtc cagcgcttcc aggcgcgggg caatcaccga
     5281 ccattcctca ctctcgtccg ttaccagcac ggagccggaa gcgggcggat tggcagccag
     5341 ctgagattgt cttgccgccc gcgcgttttc cgtggcaaag cgggccttga ccacgtacat
     5401 aaaggcgttg cggaagcgat tcaggcgcac gcggtcctcc aaccacgagg catagcgcga
     5461 aagccagcgc agcactggcg ccaggtcgga ttcgccccac tgcgcacccg ccggccgatt
     5521 cacggcgtaa tgcagcatca cgggcggcat ctccgggctg tcttgcagtg ggtcgtaggc
     5581 cgggtagcag agcgagatat cgttgagatc cgggcgggtg tggaaggtca gttcctgttc
     5641 cacatcgttg ggctgcgaag tgatgcgctc aatatccgcg gaaggcacca ccctcaggta
     5701 agacatcccg ccaggatcgg tggagagcag caggaacagg ttgccgctgc gcgccagctc
     5761 atccgaaagc tccaccaggc gcgcgtccat gcggttcagc gggtgccgcc agaacgcgag
     5821 cagaaactcc cccgtgcggc tctccgcaca gttcacgcgc attccgcctc cggtcacata
     5881 ttgggtggtc agctcgataa tccggcgcgc aagcgggtta aagcgccagg cgtccaggca
     5941 gtcgtccatc acctgaagcc ggtcccaggc atgtcggtcg cgcggttggt cgcccaaagc
     6001 gctggcgcct accaaaaagc tgttgtcgtt ttccaccagg ctcaaaccgg cgcgcagctc
     6061 cccggcgtcg gcttgctggt cctgtgcttc gattgtgctc gtcctgttcg tgcgcctaaa
     6121 gattcccata cacgtcctct ttccttttca gcttgtgggt tatttggttt tgcgctgtcc
     6181 cgcgcgggca gggtttcccg tccagtgggc aaagctgcgg cacggtctca atttgcctgc
     6241 caaagtaccg cagcaggtct tcgcttttcc cggcccagcg gttgtaatcc atgtaccagc
     6301 ccgaaccgcc gatggctggc gcgcgttctg cagtctgatg aatcagccag ctcgaaattc
     6361 ctttgggcag gcgcggcggg cagggatact cgggcgtaaa gcccgggtaa gcgcgccggg
     6421 cgtaatactg cgccagccac caatccagtt caggcaaatc cgagacctcc agatactggt
     6481 ccacccaaga cgcgcgcgag tacacaatcg gatagcggcc ggtctcttcg tccaggattt
     6541 ccaggcaggc gttcagcgtg tcggtaatcc tgcggcgggt ctgcccatgt tccagctcca
     6601 tgtccaaaac caggcgcaga tgttccaggt cttccccttc cagaatgcca aggaaagcgt
     6661 ccatctgccg ggcagcagac tcagcaggga agaccacgtg atacggcaaa acgcataaac
     6721 cgatgcgccg cgcttcctgc acataaaacg cgaaagccgg gtcgcgatat ccccaggact
     6781 gccccgtccg cagcgcggca aactccacgc gcggctggtg cgcggcaatc cgctcaaaat
     6841 ccggtctgcg cttgccgtct gcgctgctgt tgtagaccga aaggtcaatt ccaaaggcaa
     6901 attgcccgtt gacattgcaa tcgtccatca gaaggctcca tccatctcac gcaaagggtc
     6961 cgccgcctgg atgagcagcg gctcatgcgc ttctccccaa ctctgctcgt ccaacacgga
     7021 ggtcatcgca gccgaaagca ccaggtcgtc gtgcagtttg ctgccgtcca tggggtcgcg
     7081 cgcggcttcc ggcacgcccc agcggatatt gcgctccgga cccaggctga cctcatacgc
     7141 cacagcctgc agttgccggt aaaagagcgc gtgcagctgc ccctgctggc tgtgtgggtc
     7201 cgcgggcagg tagtcctgaa agcgcccggt atccacgacc gccagaaagc cccaacccag
     7261 gcgggacttt acctggcggt tgaagttgag ctgaacgacg cgctcaccca ggcggtccgc
     7321 cagaaaagag gccacccccg cgccgacgcc gctggcatcc acgaccactt tacgcggctg
     7381 ccagcgatcc accagcgcca gcaagcgcgc gtactgcgta ctgtgttttt cgccggtcca
     7441 aagctcccgg cgcgccaccc gatagacggg ttttccggtc aggtcgcccg cgcgagcgcg
     7501 gatgtccacc accacgatgg tgatggcggt ggaatctctg ccggggttgg aaagcgcaat
     7561 atccccctct ccagccggat tggcgcgcag ctgctcatcc tcccccgcca ggtccaccag
     7621 cagcgcgtaa gtcttcccgg gttcgggcgc gtcttgcact gcctgactgc cttgcatcag
     7681 ccctgtgcgc gccggcgtga agagcccgat ttccgcgtca atttcctcgc caaagtactg
     7741 gctgcgcacc atcgggtggg tcctgcccag gcgcgccacc tgttcatcca caaataggcc
     7801 gtaagccggc acttctgcgc gcaccgcctc tgcccccagg caaaacaccc ggcggatgcc
     7861 gtcgcgtgct tccgcctgcc ttgccaggcg cagctcgcgc gccagcagcg tgtgcgtcgt
     7921 ccacgcagtt ccccaaaaca cgcgcgtggc attcgtggaa gccgccatcg gcgcgatttg
     7981 tttgtcatac ttttcgatgc cgatatcctg cgcttcgtcc agctgcagca gcgtggaagc
     8041 cgttgccccg accacgttgg actccggcgc agcggacaga aaggtcaggt gcgcgttgcc
     8101 aaagcggtaa tgattcccgg cgcttttctt ccaagagccg cgggtcagcc aatttgcctg
     8161 cagggcagtc tccagccggc gcatggcgtt caggctctgc ggctggtaag tcggcgaaaa
     8221 tttgaccatc tccgcgccgc agagcgcgaa gcgcgtcagg aggtacactt ccaactgtgc
     8281 ctgcagcatg ttcttgccag attggcgcgg gaacatcacc acgatggaaa gcccgagctt
     8341 ttccaatacc gaacgcgcga tggtctcccc cgcggcatcc tgataagcgc gcaagcgcac
     8401 acctcccaaa acccggctgt agacccccag gctgcgcagg gagcgcagga aggctgcccg
     8461 ggattgaggt tccggattgg cataagctcc ggaaagaaca gcccctgccg gctgcgtttt
     8521 gtttcgcggc aggctctccg gagaatgaat caagccgcca ggcgacaaag cgctatccat
     8581 gtctgccgcc tccgggttga aggccctgac cgaagcggcg ttgaatgggc tcctgagcgc
     8641 gcggcctgcc agcttccggt tttccagatt ggcgattaaa ctgcatcttt ttgctcctct
     8701 tctgctgata ccagcgcgag ttcatccatc agtttttcca gcaaatcgtc ctttccggcc
     8761 tgtttggccg ctccttgcag tgcctggttg atcttgagca cgttccccag ccgcgtgcag
     8821 ctgaggccca gcagattcag cgcgtcgccc agccgctcga cagtctcggg gttttcgtcg
     8881 gagcgcaccg cctgaaaaaa acgcttcatc gcggtccgca gcatcacgac ctcggcatcc
     8941 aacgtttttc gggcggcggc tgccagttcc ctgcgttccc ccgggctgaa gtggtccagg
     9001 taaaaatcag catcttgcgc gcgcgcatcg cccagcagcg cacggatttg ttcgtccgcc
     9061 tccgagccca ctggcagcct gcctgcccct gaggagcagg caggcccgga gtcagacgga
     9121 tgcgtagttt gcctgggatt cttgtggttc atcgtacgct gcctcctaaa tagaacttat
     9181 gttctattta agccatatct tacaacaaaa aaaccgcttg aaaagaggtc aaaagcggac
     9241 agttttatta aatttgtccg attccggaca atctttaaca attgtggtga gtcggattag
     9301 tcataggtca ggtggagcgg cagtcgtagg tcgggtggag cggcaatcgt aggtcggatg
     9361 aagcggcagc cgtaggtcgg gtggagcggc agcgcaaccc gacaaaacat ggtcgtagtc
     9421 gtagg
//
LOCUS       DAOQAD010000070         9400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_8614_length_9400_cov_5.331621, whole genome shotgun sequence.
ACCESSION   DAOQAD010000070 DAOQAD010000000
VERSION     DAOQAD010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8614_length_9400_cov_5.331621"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(857..4081)
                     /locus_tag="PKL60_06390"
     CDS             complement(857..4081)
                     /locus_tag="PKL60_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNW95797.1"
                     /translation="MSNRSLIILSRLAPPAERSNVLPRERVTALLRGSLEYPATILQA
                     GTGYGKSTSLLTFIGQLDFPVFWFSVSADDRDPTLFLMNLYTAFDQHGFNMGAEALRI
                     LNTPDSEPTDGLIALVNAIANQIPERCLLILDDMQYVDQSPTILNLVDWFIDHLPRSL
                     HVILSTRRVLDFPSIHRWRAKGDVLELGKRDLTFTPEEVKTLFSSQYHLELNEEEVEQ
                     LLQRTEGWVIGLQMVWQSLKGQPNGTVLEMLKEESESRKNLFAYLAEEVLESQAPEYQ
                     NFLLKTSVLSFLDTETCDFLMDSQNSIDILNELYRSGLFLEKLRPGVYRYHHMFREFL
                     ESHLSRNPELERELHRKIGSYYAAHQYWESAIGHMLKVGDYPQINHILENVGRDFILT
                     NRHESVRYWIEQFPEAQRAQYPYVNFLAGEVNRYNGVFEMALEDYRTAQRTYQKAGNQ
                     WGVSLALRGQAQVYLDTIRPINANQLLRRALALLDPAENRQEVAELLTQMAENQVNQG
                     EMEQAELSLQRSREFLRSDSEENDFIQARLYLRTGRIQEGIQLLQKLEPAYELESVSE
                     SMQMSRPQRFHREASLLLSLFYTFSGELDKALRYAQRGIEIGQHYRSVFVRSVGFMRL
                     GHALQLSRTFDDDETEYMKVVGLYEESMRAVDIARIHVEPLWGMCRLLGYSGRLDEAQ
                     KVAEDALSIANSSGDIWIGMLVRLSLGASLAISGAYEAANVELSIVESIAARAGDELS
                     RSAALLWLAYCALHQGYESSLWLYLEQGLALIHQHQYQFLLTKPTMLGSDNPIVFLPL
                     LLQAQRQGLQPELTRQILEEIGAANLEYHPGYTLRIKTFGGFAVWRGPKLLESADWKR
                     EKSRQLLQVLVGNWGKWMSRDQLTLVLWPDAEPATAYNNLKVTLSSLNQALEPDRPEG
                     ARPAFVLRRGSQYMLSPNIGIILDASRFEELCRSSDPNALESAARLYQGRYFENEAAE
                     EFFIAEGQYYHEHYLQVMDQLIQGAMNAGDWEKALNFSRTVLSRDALWEPAYRSMMLI
                     HHRMGNPGSVLQIFHQCQQTLNREIGSGVSAETQALLNELLGRT"
     gene            complement(4279..4500)
                     /locus_tag="PKL60_06395"
     CDS             complement(4279..4500)
                     /locus_tag="PKL60_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95798.1"
                     /translation="MAYTFLSKVGDLLKDPYAVQVIERYVPGITKNPLIMLAKGKTLQ
                     SLLDMPQAKSAGLTNEMVTKVLAEINQKK"
     gene            4920..5663
                     /locus_tag="PKL60_06400"
     CDS             4920..5663
                     /locus_tag="PKL60_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95799.1"
                     /translation="MSAKRVWILVASVMILVGLTVVTVFANPFSQTSSEDGAHYEEWI
                     GTADTVIGDDPDEDGYTGPVVSSPGIAVPSEGESDLETLPAEPASEGEFRPDELQPEA
                     PEAEPQWSGFYYDFSAGATMRPRASLTNWEYWGGGCLYTTKGYDLLTLPLNLPEGVRI
                     DYLRLYYYDSSTAGNLVAYITIYDGGGGIADLISVVSSGSAGYGYVVSDYVGQVVDNS
                     TYAYVLNVGTGVNNETNRLCGLRVAYRLP"
     gene            5701..6282
                     /locus_tag="PKL60_06405"
     CDS             5701..6282
                     /locus_tag="PKL60_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95800.1"
                     /translation="MKKVLRSTCRLGWRIVLVGILLAGLLNAVPIGTVSAALISYEIF
                     SLQIHLPEGARIEYLRLYFYDTNTSHNSIAWITTYDGAGGYTDVVSTQSSGSSGYGQS
                     VSAYFEHIVDNETETYVLNWLPSVSDASMRLMGMRVAYKLPDGAGGWVGYYYLHVAGC
                     TFTPRSSTMEWRYPLAGGVYATSWDHFLPFLRK"
     gene            6613..7059
                     /locus_tag="PKL60_06410"
     CDS             6613..7059
                     /locus_tag="PKL60_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNW95801.1"
                     /translation="MSNPKKHQKSRRKTLPAWAWLLIFAAAAGLVYLLISPGNSNDVK
                     LPREVTVAQAAALREEGAFVLDVREPSEWEQGHIEGATLIPLGQLLQRSGELPGDQTI
                     VVVCRSGNRSAQARDILETAGFTNVTSMSGGMNDWQAEGYPLVPGP"
     gene            complement(7486..>9400)
                     /locus_tag="PKL60_06415"
     CDS             complement(7486..>9400)
                     /locus_tag="PKL60_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNW95802.1"
                     /translation="AASLEKLLPRLESAFAEQIAADPQAWLAFKQRLEEHFPALFRLY
                     HHLYAARYDFFYHLEALLSELARAAFARPADLRALDAEREENPLWFQSNTMLGGVCYV
                     DLFAGNLDGIRAKIPYFKELGLTYLHLMPLFRMPEGENDGGYAISSFREVHPPLGTMA
                     QLAQLSRALREEGISLVLDLIINHTADEHEWAERAKAGEARYEAMYGIYPDRTIPDLY
                     ERNLREIFPDEHPGAFTWFENLRKWVWTTFHSYQWDLDYANPDVFNHMAGEMLFLANQ
                     GVEIFRLDAVAFIWKELGTACENLPEAHIILQGFNALTRIVAPSMLFKSEAIVHPDEV
                     VRYISPEECQLSYNPLVMALLWNTMATRNVDLLQQALHSRFAIHPRTAWVNYVRCHDD
                     IGWTFDDADAARLGINGSDHRRFLNEFYRGRFPGSFARGLPFQENPKTGDCRISGTCA
                     SLAGLEKALAEEGPAEVELAVRRIALLYDIVMTLGGIPLIYLGDEIGVLNDYSYLDNP
                     AHKDDSRWVHRPAANAQSYSRRHDPASLEGRVYANMRAAIAFRKDHPELAGGALEALE
                     SGSSAVLMYARSCGGSRRLVIMANFSEQEQMVGTWAAQQNNLGAKKRLFGSAQPLENG
                     AVRLPPYDFSVFG"
BASE COUNT         1994 a         2536 c         2701 g         2169 t
ORIGIN      
        1 gaaaattaac tgatgatcct actgaacgat cgctgggacg gtgagttgaa acgaatttcc
       61 ctttcccagc ggcttgaccg aaccaaaata gatccgctca gggttagggc aagcgggaaa
      121 aatgatagaa aagaagaaat acggggcttc gtttcgagaa aaaaaacccg tcctgaaaac
      181 ttcatccact ccttagggat atccatttac cttcataaag ttattcttca gaaacaatag
      241 caagtatggt tagcgtctct gtaaatggtg taaatttact gcgtggaatc tttaaggttc
      301 aagggtgtca atgaacaggc gccagagtaa atggcattac ctcagccacc atcaaggatt
      361 gcggctcatt gaaaaatcaa tcttcgctgc attcagaatg acaaaaccgc agatgcttca
      421 atgatcttca gatgatgaag tattcctgcc aatcatccgg ggcaggggca aagcttcccg
      481 atgcgcgaat acagaaaact tacgcagctt cgtggagatg tcaaatatca cgaaaccaga
      541 attggttctc agactgcgta gtttcgccgg cgcttttttg gggaagtggg tcgggtattc
      601 ctgcttcgca gcactacttc aacccgaaaa aaacggcagc tgcttgacac gcaatgcttc
      661 tccggggaga ggctggcggc tgcggcgaga cggttgaggg tctccattct cagttggttt
      721 tttcaccagg tgggatgcct ttgatatccg ctgatccagg cgaatgctcc caacctgacg
      781 cggggctctg catcatgaaa ggtgcaaaat tcggacgcct gcccttgcaa ccaactcgct
      841 gtggggtgtt tcagtttcac gtccgaccca gcagttcgtt cagcagggct tgcgtctccg
      901 cgctgacccc gctgccaatc tcccggttca gcgtttgctg gcactggtga aaaatctgca
      961 gcacgctgcc tggattgccc atgcggtggt gaatcagcat catactgcgg taagcaggtt
     1021 cccacagcgc gtctctgctg agcacagttc tgctgaagtt caatgctttt tcccaatccc
     1081 cggcgttcat cgcgccttgg atgagctggt ccatcacctg caaataatgc tcgtggtaat
     1141 actgaccctc tgcgatgaaa aactcttcgg ctgcttcgtt ctcaaagtag cgcccctggt
     1201 agaggcgcgc cgcggattcc agggcgtttg ggtcggagga gcggcacagt tcttcgaagc
     1261 gtgatgcgtc gaggatgatg ccgatgttag ggctcagcat atactggctg ccgcgccgca
     1321 gcacaaaggc gggcctggcg ccttcaggcc tatccggttc aagcgcctgg ttcaggctgc
     1381 tgagggtaac tttcaggttg ttgtaagctg tagcgggctc cgcatccggc cacagcacca
     1441 gggtaagttg gtcgcggctc atccatttcc cccaattccc gaccagcact tgtaagagct
     1501 gacgggactt ctcccgcttc cagtccgccg attccagcag ctttgggccg cgccaaacgg
     1561 caaacccgcc aaaagttttt atgcgcaggg tgtagcccgg gtggtactcc aggttcgccg
     1621 cgccaatttc ctccaggatt tgtcgggtaa gctcgggctg cagcccctgc cgctgtgcct
     1681 gcagcagcag cggcagaaaa acaatcggat tatcactgcc gagcatggtc ggtttggtca
     1741 gcaagaattg atattggtgc tgatgaatca gcgccaggcc ttgttcaagg tagagccaca
     1801 aagagctttc gtaaccctgg tggagcgcgc agtacgccag ccacagcagc gccgcggagc
     1861 gtgagagttc atccccggcg cgggcggcga tggattccac aatcgaaagc tcgacgttgg
     1921 cggcttcata cgcccccgag attgccaggc tggcgcccag tgagagccgc accagcatcc
     1981 caatccagat atctccggag ctgttcgcga tcgaaagcgc atcctcagca actttttggg
     2041 cctcatccaa ccggccggaa tacccgagca ggcggcacat gccccacaag ggttccacgt
     2101 gaatccgggc gatatccaca gcccgcatgg attcttcgta cagcccaacg accttcatgt
     2161 attctgtttc gtcgtcatca aacgtacggc tgagctgcag tgcgtgcccg aggcgcataa
     2221 aaccaacgct gcgcacaaag acggagcggt aatgctgccc gatttcaatc ccgcgttggg
     2281 cgtatctcaa agctttgtcc agctcgccgc tgaaggtata gaacagggag agcagcagcg
     2341 aagcttcgcg gtgaaagcgc tgcggccggc tcatctgcat gctctcgctc acgctttcca
     2401 gctcgtaagc tggctccaac ttttgcagga gctgaatgcc ctcttgtatg cggccggtgc
     2461 gcagatacaa gcgcgcctgg ataaagtcgt tttcctccga gtcgctgcgc aaaaactccc
     2521 tggaccgctg caggctcagc tcggcctgct ccatttcacc ctgattgacc tgattttccg
     2581 ccatctgcgt cagaagttca gcgacttcct ggcggttctc cgcaggatcg agcagcgcga
     2641 gagcgcggcg cagaagttgg ttggcattaa tcggccggat ggtatccaga taaacctggg
     2701 cctgaccgcg cagcgccagg ctgacgcccc actggttgcc agctttctgg tatgtgcgct
     2761 gagcggtgcg gtaatcttcc agcgccatct caaacacgcc gttatagcgg tttacttccc
     2821 cggcaaggaa attcacgtag ggatactgcg cccgttgggc ttccggaaac tgctcaatcc
     2881 agtaacgcac gctttcgtgg cggttggtga ggataaaatc cctcccgaca ttttccagaa
     2941 tatggttaat ctgcgggtag tcgccgacct tcaacatatg cccgatggcg ctttcccaat
     3001 actgatgcgc tgcgtagtag ctgccaattt ttcggtgtaa ttcccgttca agctcaggat
     3061 tcctgctcaa atggctttcc agaaactccc ggaacatatg gtggtagcgg tagacccccg
     3121 ggcgcagctt ttccaggaac aggcccgagc gataaagctc gttcagaatg tcgatgctgt
     3181 tctggctgtc catcaggaag tcgcaggttt ctgtgtccag gaaggaaagc accgaggtct
     3241 tgagtaaaaa gttctgatat tccggagcct ggctttccag gacttcttcc gccaggtaag
     3301 cgaacagatt cttacgcgat tcgctctcct ctttcagcat ctccagtacg gtcccgttcg
     3361 gctggccttt cagcgactgc cagaccatct ggaggccgat cacccagccc tccgtgcgtt
     3421 ggagcagctg ctcgacttct tcctcgttca gctccagatg atattgggag ctgaaaagcg
     3481 tttttacttc ttcaggcgta aaggtcaggt cgcgcttgcc aagctcgagc acgtctccct
     3541 tggcgcgcca gcggtggatg gaagggaagt caagcacgcg gcgggttgaa agaatcacat
     3601 gcagggaacg cggcaaatgg tcaatgaacc aatctaccag gttcaggatc gtcggggact
     3661 gatccacgta ctgcatatca tccaatatca gcaaacagcg ctcagggatt tggttggcaa
     3721 tggcatttac aagcgcaatc agcccgtccg ttggctcaga atcgggcgtg ttgaggatgc
     3781 gcagcgcttc cgctcccatg ttgaagccgt gctggtcgaa ggcggtgtaa aggttcatca
     3841 ggaacaaggt cgggtcgcgg tcgtccgcgg agacagaaaa ccaaaaaacc ggaaaatcga
     3901 gctgtccgat gaaggtcagc agggaggtac ttttgccgta gccggtgcca gcctggagaa
     3961 tcgtggcggg gtattccaga gaaccgcgca gcaaagcggt aacgcgctcc ctggggagga
     4021 cattagagcg ctcggcaggc ggagccagcc ggctgaggat aatcagactg cggttgctca
     4081 tgttcttatt ttaccagtct gaccggatcc aagcctgaga tgcagtggtt gtttccttga
     4141 tcgggcgatt gaggtggcgc tatctgaaga ggactgccag cctgtgcttt tggccgtatt
     4201 tccggcagag atggggataa aaagctgccg gcctgtgttc cactgccggc agcctgtctt
     4261 ctaacgctct tcgtccgctt acttcttctg gttgatttcc gccaggactt tcgtgaccat
     4321 ttcgttggtc agcccggcgc tcttggcctg cggcatgtcc aggagggact gcagggtttt
     4381 gccttttgcc agcatgatga gcggattctt cgtaatcccg gggacgtaac gttcgatgac
     4441 ctggaccgcg taggggtctt tcaacaaatc gccgactttg gataggaatg tgtatgccat
     4501 ttttctcctt tgcattcact ttgttgggga attgtaacgc attacccgga tacaaggtaa
     4561 agctgcataa gaccctgaaa gcccgccagc tcgacgccag gccgattttt tgccatatgc
     4621 cgggggtggc tgcgcggata accatttact cgactgccgg ataatgcccg gcattccaat
     4681 gcaaaaaact attaatgaat ttgtcccatt gggttgatca gcctattgac attgtctgta
     4741 aattgttcta ctatgtaaat agccaatttt tttctttttt agtccgtctg tcgatttcat
     4801 ccaggttaat cacgaatatg ttttgaaagc accgatgctg tgtgctttgt ttagcgtttg
     4861 taatcgctgt tccgcgaaaa aagagttctt gattcattca ctaacctttt ggaggaaaaa
     4921 tgtccgcaaa acgtgtttgg attttggtag cgtccgtgat gatcctggtt ggattgacgg
     4981 tcgtcacggt ctttgcgaac ccgttttcac aaacttcaag tgaggacggg gcgcattatg
     5041 aagagtggat cggaacagcc gataccgtca tcggcgacga tcccgatgag gatgggtata
     5101 cgggcccggt ggtaagcagc ccgggaatcg cagtcccatc ggaaggcgag agcgacctgg
     5161 agacgttacc tgcagaaccg gcgagcgaag gtgaatttcg ccctgatgag ttgcagcccg
     5221 aggctcctga agcggaaccg cagtggtctg ggttctacta cgatttttcg gctggcgcga
     5281 ccatgcgccc gcgcgccagt ttgaccaatt gggaatactg gggcggtggg tgtttataca
     5341 ctactaaagg atatgatctg ctaacgctgc cactaaacct gcctgagggt gtccggattg
     5401 attatctgcg tctctattac tatgatagtt ccactgctgg caaccttgtg gcctatatca
     5461 ctatctatga cggcggtggc ggtattgctg atttgatcag cgtagtttct tccggttcag
     5521 caggttatgg ttacgtggtc agtgattacg tggggcaagt ggtggataac tctacctatg
     5581 cctatgttct gaatgttggt accggtgtaa ataatgaaac taatcggctg tgcggtctgc
     5641 gcgtcgcata taggctgccc tagttccggt tcgcataact caaaaatgat gaggtgaacc
     5701 atgaaaaagg ttttgcgcag tacatgtcga ttgggatggc ggatcgtgct ggtcggaatt
     5761 ttgttggcag ggctgctgaa tgctgtaccc attggcacag tcagcgccgc attaatctcc
     5821 tatgagatct tctcgttgca aatccacctt ccggaaggtg cgcggattga gtacttacgc
     5881 ctctattttt acgataccaa caccagccat aatagtatcg cctggataac cacatatgac
     5941 ggtgcgggcg gttataccga tgtggtaagt actcagtcga gcgggtcctc aggatatggc
     6001 cagtcagtta gtgcctattt cgagcatatt gtcgataatg aaacggaaac ctatgtgctt
     6061 aattggcttc cctcggtgtc ggacgcaagc atgcgtttga tgggcatgcg cgtcgcgtat
     6121 aagcttccag acggagccgg aggttgggtg ggatattact acctgcatgt ggctggctgc
     6181 acctttaccc cgcgctccag caccatggag tggcgctatc cattggctgg cggggtttat
     6241 gccaccagct gggatcactt cctgcccttc ctgaggaagt aagcgccaga ggcaggagtt
     6301 aagccagaag tcatattccg ccggctaagg tttgccagag ttataggatc ttgagagcag
     6361 cggttcgcac ctggaccgct gcttagtcaa tttccggctc tggcgtatca gcagcggcag
     6421 cattttccgc ttatcagcgc gggccggatt ttgtgtgtgc cgcgcatgac tcccaagcct
     6481 gcgcctgggg cgcggtcaag gcgatgctga ttttcccgtt gctcctgccc gagcggcgcg
     6541 gcggcgtaat cgggcgcgca atggaacgcg gcacgaaaag tgctgcgcga tttacccgga
     6601 agaagatgaa tagtgagcaa cccgaagaaa caccaaaaat cccgccgaaa aaccttgcct
     6661 gcctgggcct ggctgctgat atttgcggct gccgccgggc tcgtttatct tttaatatcg
     6721 cctggcaatt ccaacgacgt gaaactcccg cgcgaggtca ctgtcgctca ggcggcagcg
     6781 ctgcgggaag agggagcctt cgttttggac gtgcgtgagc cgagcgagtg ggagcagggg
     6841 catattgaag gcgcgacttt gattcccctg ggtcagcttt tgcagcgttc cggggagctg
     6901 cccggcgacc aaaccatcgt ggtggtttgc cgttccggga accgcagcgc gcaggccaga
     6961 gatatcctgg aaacagccgg attcacgaac gttacgtcca tgagcggggg aatgaatgac
     7021 tggcaggcag agggctaccc gcttgtccct ggaccgtagg ttggttttta gcaaagtaga
     7081 ggcgggacac tcattgggtt tcccgctttt tactccgacc atcggcatgg tcgtttggcg
     7141 cggcatgctg gtggagtgca gcgacgcagg agcctaaccc aacattgata attagcaggt
     7201 cgtgtcaggc tgcactctac aaatcatcat gctgatagaa gcccgctgcc gctccaccca
     7261 tacaaacgct tacctatctg cctttacaat gttatctggt gaggcgcagc atcccggata
     7321 agcgcagctg gtgcgtaagg ttctgccagg ggcgtttcgc ctctgtgctt gatgccagcc
     7381 agaagggtca ggttgtgctt ctcacctgac ccggcggtcg agcagctcgg attgcgaggc
     7441 aatagcttcc agcggcgcct tgtcccgcgg cgggcaagct ttccgctacc caaacaccga
     7501 aaaatcatag gggggcagcc gcacggcgcc gttttcaagc ggctgcgcgc tgccgaacag
     7561 gcgttttttg gcgccgagat tgttttgctg ggcggcccac gtgcctacca tctgctcttg
     7621 ttcgctgaaa ttcgccataa tgaccagacg gcgcgagcca ccacacgagc gggcgtacat
     7681 caacacagcg ctgctgccgc tttccagcgc ttccagcgct ccgcctgcca attcggggtg
     7741 gtccttgcgg aaggcaatcg ccgcgcgcat gttggcgtat acacggcctt ccaggctggc
     7801 cgggtcgtgc cggcgggaat aactctgtgc gttcgcggcc gggcggtgca cccagcggct
     7861 gtcatccttg tgcgcggggt tgtccaggta gctgtagtcg ttcaacacgc cgatttcgtc
     7921 tcccaggtag atcagcggaa tgcccccgag cgtcattacg atgtcataca gcagtgcaat
     7981 ccggcggaca gccagttcca cctcggccgg gccttcttcc gccagggctt tttccaaccc
     8041 cgccaacgaa gcgcaggtgc cagagatgcg gcagtcgcct gttttggggt tctcctgaaa
     8101 gggcagtccg cgagcgaagc tgccggggaa gcgcccgcga taaaactcat tcaaaaagcg
     8161 ccggtggtcg ctgccgttaa ttcccaaccg ggcggcatcg gcgtcgtcga aggtccagcc
     8221 gatatcatca tggcagcgca cgtaattgac ccaggcggtg cggggatgga tggcaaaacg
     8281 gctgtgcagc gcctgttgga gtaaatctac attgcgcgta gccattgtgt tccacagcag
     8341 cgccatgacc agcggattgt aggaaagttg gcattcctcc ggcgatatgt agcgcacaac
     8401 ctcatccggg tggacaatcg cctcggattt aaacagcatg gacggcgcga cgatgcgagt
     8461 gagggcatta aatccctgca ggattatgtg cgcttcgggc aagttttcgc aagcagtccc
     8521 caattccttc cagatgaagg cgaccgcgtc cagccggaag atttccacgc cctggtttgc
     8581 cagaaagagc atctcgcccg ccatatggtt gaacacatcc gggttagcgt agtccaggtc
     8641 ccactggtac gagtgaaaag tagtccagac ccactttcgc aggttctcaa accaggtaaa
     8701 cgccccgggg tgttcgtccg ggaagatttc gcgcaggttg cgttcgtaca aatccgggat
     8761 agtccggtcc gggtaaatgc cgtacattgc ttcgtagcgg gcttcgccag ctttggcgcg
     8821 ttctgcccat tcgtgctcgt cagcggtatg gttgataatc aggtcgagta ccaggctgat
     8881 gccttcttcc cgcagcgcac gggaaagttg tgccaactgc gccatcgtgc caagcggggg
     8941 atgcacttcc cggaagctgc tgatcgcata gccgccatca ttttcacctt caggcattcg
     9001 gaaaagcggc atcaggtgca gataggtgag cccaagctcc ttgaagtagg gaattttcgc
     9061 gcggattcca tccagattgc cggcaaagag atccacataa cacacgccgc caagcatggt
     9121 gttggactgg aaccagagcg gattttcttc gcgttccgcg tccagcgcgc gcaggtctgc
     9181 cgggcgtgcg aatgccgcgc gagccagttc agaaagaagg gcttccagat gatagaaaaa
     9241 gtcgtagcgc gcggcgtaca gatggtggta gagccggaaa agcgccggga agtgctcctc
     9301 gaggcgctgc ttgaaagcca gccaggcttg cgggtccgcc gcgatttgtt cagcgaaagc
     9361 cgattcgagg cgcggcaaga gcttttccag gctggcagcg
//
LOCUS       DAOQAD010000071         9369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_8654_length_9369_cov_5.297402, whole genome shotgun sequence.
ACCESSION   DAOQAD010000071 DAOQAD010000000
VERSION     DAOQAD010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8654_length_9369_cov_5.297402"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PKL60_06420"
     CDS             <1..642
                     /locus_tag="PKL60_06420"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HNW95803.1"
                     /translation="QLEEEFLIFHLRMSGDLSMRFLPPEQPFQPAAHDRLILTFASGW
                     ALVFNDTRKFGRAWLTMDPRSVIGSLGPEPLDEALTPEIFHAMLRVRSRQLKPLLMDQ
                     RFLAGLGNIYTDEALFCAGLHPLRLSSSLTQSEAAALLAAIRQNLLQGILEHGSSIDW
                     VYRGGSFQNHFQVYQRSGQSCLRCGAPIQKITVGQRGTHFCPVCQPKGQQENV"
     gene            635..1408
                     /locus_tag="PKL60_06425"
     CDS             635..1408
                     /locus_tag="PKL60_06425"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW95804.1"
                     /translation="MYDKFASQYDFFVNWKSRLAYEMPFIEEQLALLGGLPEQHRLMD
                     SACGTGQHAIALARLGYQVSGTDLFPQMVNLANTNALAADVNIRFKTAGFGSIAAAFP
                     GESFDAVFCLGNSLPHVNSLNDLQHTLEDFHACMAPGALLLLQQRNFDAVMRAKNRFM
                     DPQTHISGDEEWLFFRFYDFEPDGKIQFSILTLTRKPGAPWTSALTSTFLLPILHADL
                     KKALENVGFSDLRFYGNMKAEEFDPATSGDLIATARKNT"
     gene            complement(1452..2468)
                     /locus_tag="PKL60_06430"
     CDS             complement(1452..2468)
                     /locus_tag="PKL60_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNW95805.1"
                     /translation="MILVTGAGGHLGNVLVRELVARNEKVRAMVLPGEDLTSLEGLDV
                     EIFEGNILDKARLFEACEGVDLVFHLASVVAITEDKVDLMRQVNVEGTRNVIDAVRQA
                     GVRRMVYTSSIHALQRPDPEVTIDESLQFDPENPAGPYDRTKAQATLLVLNAASQGLD
                     AVVVCPTGVIGPYDFKRSELGEMMLSWMKKGPSLSVQGKFDFVDVRDVALGHILAAEK
                     GKSGETYLLSGEQLQISVMRSLVQQAAGIDSAEIKFPARLAAFVAPLAEFFYRLTRTK
                     PKFTRYSIETLRSNSKISSYKAQRDLGYRPRRLYDTIKDTVDWWKINVHKTISALRTK
                     PQKF"
     gene            complement(2473..3060)
                     /locus_tag="PKL60_06435"
     CDS             complement(2473..3060)
                     /locus_tag="PKL60_06435"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNW95806.1"
                     /translation="MARLQKSREKILEMAGRLFSEQGYDATSVHEICMKAGVSKGAFY
                     HYFSTKENLFLILMEDWLERMKQVLVGEGDKRAAFPESLLEMAGRSGEIFLTTQNHFS
                     VMIAFWKEALRDPNVWARSLQPCEDLLELIQEQVTVGQQAGFVRGNLDPGAAAHLLSA
                     CTLGYLLQAALCPEQKDWPALARSGFELVVNELRS"
     gene            3348..3911
                     /gene="rpiB"
                     /locus_tag="PKL60_06440"
     CDS             3348..3911
                     /gene="rpiB"
                     /locus_tag="PKL60_06440"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNW95807.1"
                     /translation="MKLAIGCDHSGLELKSTLMNYLDSRLIPYEDFGTFSTESCDYPR
                     IAEKVCKAVLSGQYDRGILICGTGIGMSIAANKFAGIRAAACSEPYSAILAKRHNNAN
                     VLCLGARVVASGLATLIAEGWLNASYEGERHQRRLDMITRLEQRNAVPPYTSSASAGA
                     AADLEPHSQKPASDQEDIGMEQGAQPL"
     gene            4005..4685
                     /locus_tag="PKL60_06445"
     CDS             4005..4685
                     /locus_tag="PKL60_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNW95808.1"
                     /translation="MRYRIFGDKLPAVTIEFESGESIFTQAGGMTWMTDNFTMETNMK
                     GGMMKGLGRMLTGESLFMATYTARAPKAELTLASSFPGNILPLELDGSKTWIAQKSAF
                     LCATPGVELSMFVSKFKAGLFGGEGFLLQKLTGRGIAFLEIDGSMVEKDLQPGERIKV
                     DTGNVAAFESTVKYDVETVKGFTNILFGGEGLFLTVLEGPGKIYLQTVNVSGFAARLR
                     PFLPNKTS"
     gene            complement(4794..6425)
                     /locus_tag="PKL60_06450"
     CDS             complement(4794..6425)
                     /locus_tag="PKL60_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNW95809.1"
                     /translation="MDNAQDWHKKRTYTSEELELAKAALEDIRAGAELQKALRRHPLP
                     DGQGFLAKHTLVAAYRELVRSGAWQEDAELLARIRMKPVRTLSGVTTITVLTKPFPCP
                     GECIFCPSETDMPQSYLPDEPGARRGVENEFDPYRQVSSRLKALREVGHPTDKIELLI
                     LGGSWSAYPREYREWFTRRCFEALNEENPQDDQPEAKLVDAQQRNETGAHRCVGLVLE
                     TRPDLITREELIFNRRLGATKLQIGIQSMDENVLALNRRGHSAAQAIEAARLIRAAGY
                     KLVAHWMPNLLGATLESDRADFARLWETGSIQPDELKIYPCQLLRSAELYRYWERGDY
                     QPYTEAELISLLADIKPGVPRYCRINRVIRDIPSPNVVEGNRNTSLRQDVARELERRG
                     TPCACIRCREVRRGQVDVGALRLNDLVYGTASTEEHFLSFDTREDKLAGFLRLSLPLE
                     TNALRLADLEGAAIIREVHVYGQSIEVGTGLPGAAQHVGLGSRLLLEAERISREGGYA
                     NLAVISAVGTRRYYAARGFEPRELYMVKPLVSASA"
     gene            6488..7225
                     /locus_tag="PKL60_06455"
     CDS             6488..7225
                     /locus_tag="PKL60_06455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW95810.1"
                     /translation="MRPQTRQKLLEINRLFYERSAATFSATRRKVQPGVARLLKTLPS
                     RADILDLGCGNGNFAIALAKSAWSGVYLGIDASEALIADARAELERARPGGSFSFLTG
                     DLSGENWPSRIPLQNFPIITCFAALHHIPDKNYRAALFQRTTGLLAPEGQLLLSVWQL
                     FNDPRMSAHVLPWNAVEIDEGDLETGDYLIDWRAGGHIDQRYVHVFTPEELRALGFAA
                     GLELQDEFSSDGKSGKLALYQIWRKPA"
     gene            complement(7232..8044)
                     /locus_tag="PKL60_06460"
     CDS             complement(7232..8044)
                     /locus_tag="PKL60_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNW95811.1"
                     /translation="MLEFFSSLSQILQVLLATAFTYAMTTAGAAGVFFGKEINVKLMN
                     GMMGFAAGVMIAASYFSLLAPAIELSGRLNTPVWVPAVIGFLLGGGFLYGVDKLLPHL
                     HPGEKDPEGIKTSWQRSVLLVFAITLHNIPEGLAVGVAFGAVHAGLSGATLSSAIALA
                     LGIGIQNFPEGMAVSMPLRRENLSRGKAFFWGQLSGLVEPIAAVIGAFAVLTMRDMLP
                     YALAFAAGAMIYVVAEELIPEAKNSAVDHSDVPTIGVMLGFATMMLLDIALG"
     gene            8090..9142
                     /locus_tag="PKL60_06465"
     CDS             8090..9142
                     /locus_tag="PKL60_06465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW95812.1"
                     /translation="MNIPPKAPDFPAAADEALRKLLQACLDARRPLITEGSKSALRLF
                     QGFYEGLPGLVVDLYAGTLVITNHSRDSESLESAVRTALAFYRQQIPWIHCGLVKRRA
                     VRDTALQRGEVAFGGEPAAEIEEDGVRYALNLRLNQDSSFYLDTRLLRTFLRENSGGK
                     SVLNCFAYTGALGIAALAGGARSVLQTDMNADALRLARRSFELNRFAGSMNLLPLDFF
                     KAVSRLKTSKALFDCVILDPPLFSSTEAGTVSLTENWLTLVNKVRPLVADGGRLIAIN
                     NALYLPGAELAASLEEITQSGYVELEARIPVPQDVCGYPETIRNAPPVPPAPFNHPTK
                     INVLRVRRKDGAKANR"
BASE COUNT         2022 a         2766 c         2596 g         1985 t
ORIGIN      
        1 cagctggaag aggaattcct gatttttcac ctgcgcatga gtggagacct gagcatgcgt
       61 ttcttgccgc cggagcagcc tttccaaccc gcagcccacg accggcttat cctgaccttt
      121 gcgtccggat gggcgctcgt gttcaacgat acccgcaaat tcgggagagc ctggctcacc
      181 atggacccgc gcagcgtcat tggctcttta ggtccggaac ctctggacga agcgcttaca
      241 ccagaaatct tccatgccat gctccgggtg cgctccaggc agcttaagcc cctgctgatg
      301 gatcagcgct ttctggcagg gttgggcaac atttatacgg atgaagcttt gttctgcgcc
      361 gggctgcacc cgctgcggtt aagcagctct ctcacacaat cggaagctgc tgcgctgctg
      421 gcagccatcc gccagaatct gctgcagggt atcctggagc acggctccag cattgattgg
      481 gtttaccgcg gcggcagctt ccagaaccat ttccaggtat accagcgcag cggccagagc
      541 tgtttacgct gcggagcgcc tatccaaaaa ataactgttg gtcagcgcgg gacgcatttt
      601 tgcccggtct gccaaccgaa aggacagcaa gaaaatgtat gataaatttg cctcccaata
      661 cgattttttc gtgaactgga aatcccgtct ggcgtatgaa atgcccttca tcgaggaaca
      721 gctcgcgctc ttgggcggat tgccggagca gcacagactg atggacagcg cttgcgggac
      781 cggtcaacac gcgattgccc tggcgcgcct gggataccag gtctcgggga cggacctgtt
      841 cccccagatg gtgaatttgg cgaacaccaa cgccctcgct gcggatgtga atatccgctt
      901 taagactgcc ggatttggca gtatcgcagc cgcgttcccc ggggaaagtt tcgacgcggt
      961 cttttgcctc ggcaactcct tgccgcatgt gaactcgctg aacgacctgc agcacaccct
     1021 ggaagatttc cacgcctgta tggctcccgg cgcgctgctc ctgctgcagc agcggaactt
     1081 cgatgcggtc atgcgcgcaa aaaaccgctt tatggacccg caaacccaca tctccggcga
     1141 cgaagaatgg ttattcttcc gtttctacga ctttgagcca gacggaaaaa tccaattctc
     1201 catcctgacc ctgacccgga aacccggcgc cccctggaca agcgcgctta cttctacctt
     1261 cctgcttcca attctccacg cggacctcaa gaaggctctg gaaaacgtcg gtttcagcga
     1321 tttgcgtttc tacgggaata tgaaagccga ggaattcgac cctgccacca gcggggattt
     1381 aatcgcgacg gcgcggaaga atacttaatc cgtgcagccg gcatccgcag gctccgaatt
     1441 tcgcaagacc actaaaactt ctggggtttc gtccgcaaag ccgatatggt tttgtggacg
     1501 ttaattttcc accaatccac cgtatccttg atggtgtcat aaagccggcg gggtcgatat
     1561 cccaggtccc gctgcgcttt gtaggacgaa attttggaat tgctgcgcag agtctcgatg
     1621 gaatagcgcg taaacttggg tttcgtgcgc gtaagcctgt aaaaaaactc cgcaagcggc
     1681 gccacaaacg cggccagccg cgcggggaac ttgatttccg cgctatcgat tccggcggct
     1741 tgctgtacca aactgcgcat gacgctgatt tggagctgct cgcctgaaag caggtaagtc
     1801 tcgccgcttt tccctttttc ggcggccagg atatgcccca acgcgacgtc gcgcacgtcc
     1861 acaaagtcga acttgccttg caccgagagc gacgggcctt ttttcatcca ggagagcatc
     1921 atctcgccca gttcggagcg cttaaaatcg tagggtccaa tcaccccggt tgggcatact
     1981 actacagcat ccaatccctg gctggccgcg ttcagcacca gcaaagttgc ctgtgcttta
     2041 gtacggtcat aaggcccggc ggggttttcc gggtcgaatt gcaggctttc atcaatagtt
     2101 acctccgggt ctgggcgctg caaggcgtgg atggagctgg tgtacaccat ccggcgcacg
     2161 ccagcctggc gcaccgcgtc gatcacgttg cgcgtgccct ccacgttcac ctggcgcatc
     2221 aaatcgacct tatcttcggt gatcgccacc accgaggcaa gatggaaaac aagatctacc
     2281 ccctcgcacg cttcgaaaag gcgggcttta tccagaatgt tgccctcaaa gatttccaca
     2341 tccaaccctt cgagggaggt aaggtcttct cccggaagga ccatcgcgcg cactttctca
     2401 ttccgcgcaa ccaactcacg tacgagcacg ttgccaaggt gtccgccggc gccagttacc
     2461 aaaatcatag gttcaactcc ttagttcatt aacaaccaat tcgaatccac tccgcgcgag
     2521 agcaggccag tccttttgct ccgggcacaa cgctgcctgg agcaaatacc ccagcgtgca
     2581 ggctgacaag agatgcgccg cggctccagg atcaagattg ccgcggacaa aacctgcttg
     2641 ctggccgacc gttacctgct cctgaatcag ctctaacaaa tcctcgcagg gctgcaggct
     2701 tcgcgcccag acattcgggt cgcgcagggc ttctttccag aaagcaatca taactgaaaa
     2761 atgattttgc gtggttagaa agatttcacc tgaccttcca gccatctcca gaaggctctc
     2821 cgggaaggcc gcgcgtttat cgccctcccc gaccagcacc tgtttcatcc gctccaacca
     2881 atcctccatc aggattagaa aaagattctc ttttgtagag aaatagtggt aaaacgcgcc
     2941 tttgctgacc cccgctttca tacaaatctc gtgaacgctg gtcgcgtcat agccttgttc
     3001 tgaaaacagg cgcccagcca tctcgaggat tttttccctt gacttctgta gtcttgccat
     3061 tttgccacga cctttccaaa catcagatga ttgttgaacg ccgtaggact ggagaatgtg
     3121 tcagtatgct gccaaagtaa aaaaacctat taaagttctg aaccttttta aaacaattta
     3181 tcaatggatt gacagaatat tttcttggat agattcatta tacggttttt tttacagaaa
     3241 agaacagctt ttttgaaaga aaaaacgatt cttccacagg aatcaagccc gggttttggg
     3301 tataatcaac cgagggacga acaaaaccag agaagaagag gacgcgtatg aaactcgcca
     3361 ttggttgtga ccactccggg ttggaattga aaagcaccct gatgaattac ctggacagcc
     3421 gcctgatccc ctacgaagat tttggcacat tctcaacgga aagctgcgat tacccgcgca
     3481 tcgccgagaa agtatgtaaa gctgtgttat ccggtcaata tgaccgcggc atcctcatct
     3541 gcggtaccgg catcgggatg tccatcgcgg cgaataaatt cgcgggcatc cgcgcggctg
     3601 cttgcagcga gccgtattcc gccattcttg ccaaaaggca caacaatgcc aacgtgctct
     3661 gcctcggagc aagggtggtt gccagcggcc ttgccacgct gattgctgaa gggtggctga
     3721 acgcgagcta cgaaggcgaa cgccaccagc gccgtctgga tatgattacc cgcctcgaac
     3781 agcgcaatgc cgtcccgccc tacacctcct ctgcatccgc tggagccgcc gcggatcttg
     3841 agccgcacag tcagaaaccc gcatcggatc aagaagatat cgggatggaa cagggtgcgc
     3901 agccgcttta atccagtttt ccatagagct tttacagcgc aaagacaaag gtttgcgata
     3961 tacttttcct gttaccccac aagttcaaca aaaatggaga cactatgaga taccgtatat
     4021 ttggagacaa acttcccgct gtaaccattg aattcgaatc cggcgagagc atcttcacac
     4081 aggccggggg catgacctgg atgactgata acttcaccat ggagaccaac atgaaaggcg
     4141 gcatgatgaa gggattgggc agaatgctga ccggtgagtc gctcttcatg gccacctaca
     4201 ccgcccgcgc gcctaaagcc gaactgacgc tggcatcttc cttccccggc aacattttgc
     4261 ctctggaact ggacggttcc aagacctgga tcgcgcaaaa atccgccttc ctgtgcgcca
     4321 cacccggcgt ggagctctca atgtttgtga gtaagttcaa agccggcctg tttggcggtg
     4381 agggcttcct cctgcagaag cttaccggcc gtgggatcgc gttccttgaa attgacggca
     4441 gcatggtgga aaaagacctg cagccaggcg aacgaattaa ggtggataca ggcaacgtgg
     4501 cagctttcga aagcaccgta aagtacgatg ttgagaccgt caaaggtttc accaatatcc
     4561 tctttggcgg cgaaggcttg ttcctgaccg tgctggaagg tcctggtaaa atctacctgc
     4621 aaactgtgaa tgtatccggt tttgccgcgc gtctgcgacc tttcttacca aacaagactt
     4681 cctaaacccc cgatgactga aaacaggctg tttgtcatac gacagcctgt ttttttgttt
     4741 ttatttcccg ggaatttagt gagcgcaaag ataagagact gccggtttgt ggattacgcg
     4801 gacgcggaga ctaacggctt gaccatataa agttcccttg gttcgaaacc gcgcgcggcg
     4861 taatagcggc gcgtaccgac cgcggaaatc acagccaggt tggcatagcc gccttcccgg
     4921 gagatgcgtt ccgcttccag cagcaggcgg ctgcccaaac caacatgctg cgcagccccc
     4981 ggcagcccgg tgcccacttc aatggactgc ccgtatacgt gcacttcacg gattattgcc
     5041 gcgccttcca ggtcagccag gcgcagcgcg ttggtttcca aaggcagcga aagccgcaag
     5101 aaaccggcca gtttatcctc gcgggtatcg aaggacagga agtgctcctc ggtgctcgcg
     5161 gttccgtaaa ccaggtcatt caggcgcagc gctcccacat ccacctggcc gcggcgcact
     5221 tcccggcagc ggatgcacgc gcagggcgtt ccccggcgct caagctcccg ggccacgtcc
     5281 tgccgcaggc tggtgttgcg gttgccctcc acgacgttcg gcgaaggaat atcccggatg
     5341 acccggttga tacggcagta gcgcggcact ccgggtttga tatccgccag caggctgata
     5401 agttctgctt ctgtgtaggg ctgatagtcc ccccgctccc aatagcggta aagctcggcg
     5461 ctgcgcagaa gttggcaggg gtaaatcttc aattcatcgg gctggatgct gcccgtttcc
     5521 cacaagcgcg cgaagtccgc ccggtcgctc tcgagcgttg cgccgagcag atttggcatc
     5581 caatgcgcca ccagcttata cccggcagca cggatcaggc gtgccgcttc aattgcctgt
     5641 gccgcgctgt gcccgcgccg attgagcgcc agcacgtttt catccatgct ctggatgccg
     5701 atttgcagtt tggtcgcgcc cagccgacgg ttgaaaatca actcctcacg cgtaatcagg
     5761 tcgggccgcg tctcgagcac caatccgacg cagcggtgcg cgcccgtctc attgcgctgc
     5821 tgagcgtcca ccagcttcgc ttcgggctgg tcatcttgcg ggttttcttc attgagcgct
     5881 tcgaaacagc ggcgtgtgaa ccattcgcgg tattcccgcg ggtaggcgct ccaactgccg
     5941 cccagaataa gcagctcaat cttatcggtg gggtgcccga cttcgcgcag cgctttcagg
     6001 cgggaggaaa cctgacggta ggggtcgaat tcgttttcca cccctcggcg cgcgcccggc
     6061 tcatccggca aataggactg cggcatatcc gtttcggatg ggcagaagat gcactccccc
     6121 gggcaaggga aaggtttggt gagcacggtg atggtggtga cgccggagag cgtgcgcacg
     6181 ggcttcatcc tgatgcgcgc gagcagctcc gcgtcctcct gccatgcccc cgagcggacc
     6241 agttcgcggt aggcagctac cagggtatgc tttgccagaa aaccctgccc gtctggcagc
     6301 ggatgtcggc gcagcgcctt ctgcagctca gcgcccgcgc ggatatcctc cagcgcggct
     6361 ttcgccagct ccaactcctc gcttgtgtag gttcgctttt tgtgccaatc ctgcgcgtta
     6421 tccatgtgct tattttacct gttcccaggc gcggtatttt acctgccgaa cctgttatac
     6481 tctctttatg cgcccccaaa cccgccagaa actgctggaa attaatcgtc tgttctatga
     6541 acgcagcgcc gccaccttct ccgcgacgcg cagaaaagtc cagccggggg ttgcccgctt
     6601 gctaaaaacc ctcccaagcc gggcggacat cctggacctg ggctgcggca acggtaactt
     6661 tgccattgca ctcgcgaaaa gcgcttggtc gggcgtttat ctgggcatcg acgcctccga
     6721 agcgcttatc gcggacgcgc gcgcggagct cgaacgagcc cggccaggag gcagtttttc
     6781 attcctgact ggcgacctga gcggtgaaaa ttggccctcc aggataccgc tgcaaaattt
     6841 ccctattatc acctgtttcg cggcgctgca ccatatcccc gacaaaaatt accgcgcggc
     6901 cttattccag cgcacgacag gcttgcttgc gccggaaggg cagctgctgc tctcggtctg
     6961 gcagcttttc aacgatccgc gcatgagcgc gcacgtcctg ccctggaacg ctgtggaaat
     7021 cgacgaggga gacctggaaa ccggggatta tctgatagac tggcgcgcag gcgggcatat
     7081 cgaccagcgc tatgtgcacg tgtttacccc ggaggagctg cgcgcgctcg gctttgccgc
     7141 cgggctggaa ctgcaagacg agttctcttc ggatgggaag agcgggaagc tggcgcttta
     7201 tcagatctgg cgcaagccgg cctgatggtc cttagcccag cgcgatatcc agcagcatca
     7261 tcgtggcaaa tcccagcatc acgccgatcg taggcacatc ggagtggtca acggcgctgt
     7321 ttttcgcttc cggaatgagc tcttccgcca ctacataaat cattgccccg gccgcgaagg
     7381 ccagcgcgta cggcagcata tcgcgcatgg tgaggaccgc aaaggccccg atgaccgcag
     7441 caatcggttc caccaggccg gaaagctgac cccagaagaa agctttgccg cggctgagat
     7501 tttcccggcg cagcggcatg gaaaccgcca tcccttccgg gaaattctgg atgccgatgc
     7561 ccaacgccag cgcgatagcg gaactgagcg ttgccccgct cagaccggca tgcaccgccc
     7621 cgaaggccac gcccaccgcc aagccttccg ggatgttgtg cagggtaatc gcgaacacga
     7681 gcaagacgct gcgctgccag gaggttttga tgccttccgg gtccttctca cccgggtgaa
     7741 gatgcggcag cagtttatcc acgccgtaca aaaagccccc acccaggagg aaaccgatta
     7801 cagccggcac ccacaccggc gtattcagcc gtcccgaaag ctcgattgcg ggcgccagca
     7861 gtgaaaagta actggcagca atcatcacgc cagccgcgaa acccatcatg ccgttcatca
     7921 gtttgacatt gatttctttg ccgaaaaaaa cgcctgccgc gccagcggtg gtcatcgcgt
     7981 aggtaaaagc agtcgccagc agcacttgta aaatctgaga gagggaagag aagaattcga
     8041 gcatacgcca cctgaaaatg ataagattga actggagcaa ttgtacctca tgaacatccc
     8101 tcccaaagcg cctgattttc ctgccgcggc agatgaggct ctgcgaaagc tgctgcaagc
     8161 ctgcctggat gcccgccgtc ctttgattac tgagggcagc aaaagcgcgc tgcggttgtt
     8221 tcaggggttt tatgaggggc tgcccggcct tgtggtggat ttgtacgccg ggacgctggt
     8281 cattaccaat cactcccgcg attctgaaag cctggaaagc gcagttcgga cagcgctcgc
     8341 gttttacagg cagcagatac cgtggattca ctgcgggctg gtgaagcgcc gcgcagtcag
     8401 ggatacagct ctgcagcgcg gagaggttgc attcgggggg gagcccgccg cggaaatcga
     8461 agaggacggc gtgcgctacg cgctgaacct gcgcctgaac caggacagca gcttttacct
     8521 cgatacgcgc ctcctgcgga cttttctgcg cgaaaactca ggcgggaagt ccgtgttgaa
     8581 ctgctttgcc tacactggcg cgttggggat tgccgccctg gcaggcggcg cgcgttcggt
     8641 tttgcagacg gacatgaacg cggacgcgct gcggctggca aggcgttcct ttgaattgaa
     8701 tcgatttgcg ggcagtatga acctgctgcc gctggatttt ttcaaggcgg tcagcaggct
     8761 gaaaaccagc aaagcgctgt ttgactgcgt tattctcgac ccgccgttat tttccagcac
     8821 ggaagccggc acggtcagcc tgacggagaa ttggctgacg ctggtgaaca aggtgcgccc
     8881 gttggtcgcg gacggcgggc ggctgatcgc gatcaacaac gcgctctacc tgcccggcgc
     8941 ggagttggct gccagtctgg aggagataac ccagtccggg tatgtggaat tggaagcgcg
     9001 catcccggtg ccgcaggacg tatgcggtta tcccgagaca atccgcaacg cgccgcccgt
     9061 cccgcctgct ccgttcaacc acccgaccaa gatcaacgtg ctgcgcgtac ggcgcaagga
     9121 cggcgcgaag gcgaacaggt gaaacccggt aagagcgggc gattagtccg gcgagccgcg
     9181 gcgaggaata aacagcggcg cgagaaatga gctatctatt ttctggtctg gacagccaga
     9241 attggatttg acaagtattt ttccttatgc taaaatctgt gccacttcag ccaacgagct
     9301 gagagaatga cgagcgcggg tgtagttcaa ttggtagaac gtctccttgc caaggagaag
     9361 gtcgtgggt
//
LOCUS       DAOQAD010000072         9266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_8788_length_9266_cov_5.937683, whole genome shotgun sequence.
ACCESSION   DAOQAD010000072 DAOQAD010000000
VERSION     DAOQAD010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8788_length_9266_cov_5.937683"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1884
                     /locus_tag="PKL60_06470"
     CDS             196..1884
                     /locus_tag="PKL60_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW95813.1"
                     /translation="MKRKTVFTVFGLLIVFSMLLSACAKTGTKAPAATTKDTFIFGRG
                     ADSVQLDPSIVTDGESFRVTGQVLDSLYKFDKGTTNPVPALAECTANEDATVWSCKLL
                     EGVKFHDGTDFNADAVIFNFERWRFTTHPNHYASQVFEYYEAMWGGFDDASIITEVKK
                     IDDHNVQFILSSPMAPFLANLAMDMFAISSPTAIQKAGEAYGTPTGGCVGTGPYKFVS
                     WEEGTEIKLVANEDYWGEKPKVKNIIVRVIPDDSARFLALQAGDIDALEQAVVEDLAT
                     AEADPNLQILTRPGLNTSYLAFNFKIKEFQDVRVREAVAHAIDIEGLIKNFFGKYGTV
                     AKNLLPVGMLGYNDSIDYWTYDPELSKSLLADAGFPNGLSEVTIAEDVQDADGNVVYT
                     AGQVIPLRLYYMPVTRFYFPAAKEVGEGIAANLTAAGINVELYLEGDWPTYLGSRRNG
                     LLMGLYLLGWGGDNGDPDNFTGYFFGSGTEPIQREGWYQNAELAAILQRAVTLPDPAE
                     RAKLYSQAEQIMHDEVMRIWLGHNNTPLILSAKVKDYIPQPVGAENFEYVYFSD"
     gene            1978..3030
                     /locus_tag="PKL60_06475"
     CDS             1978..3030
                     /locus_tag="PKL60_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95814.1"
                     /translation="MAKYILRRLISVIPTLIGVTLVIFLFQRLIPGDPAVAMLGEHAT
                     EENVARIREQFGLNRPAFLDRDALVEGDLPGFFDSQYVRYFNRLLHFDLGDSIHRRIP
                     VAETLKERFPATLELALLSMFFALLIGIPVGITSASRRNSWLDGTTMIGSLLGVSIPI
                     FWLGIMEIMLFSVFLKWLPSGSRLSPGMSITPITNLLLLDSLLTGNFAGFWDALKHII
                     MPAVSLATIPTAIITRMTRSSMLDVLQEDYIRTASAKGLTERVVLYRHALKNAFLPVI
                     TIIGIQAGSLLAGAVLTETIFSWPGIGKWVYDSILGRDYPIVQGVTLLIAVIFLVVNL
                     LVDISYALLDPRISYD"
     gene            3039..4271
                     /locus_tag="PKL60_06480"
     CDS             3039..4271
                     /locus_tag="PKL60_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95815.1"
                     /translation="MSTQMPEVEVLTDFAVREHRSLWLDAWRRMKASRTAMLGLVVVV
                     VFLLSAVGAHLFWNYDAKTDLDYSAKLLPPNLVSSTENPNVHIFGTDKLGRDIFTRVI
                     HGGWNSLRVGLVAVGISLIIGCLLGLFSGFFESFEMNRIEKLVLMSGLGLALGLLPAW
                     IASQWYQTLLFGGLGAAAVFVEKKLAQSKLRPVLFAAAGALLGGLSGLLLAPLVGLVC
                     GVVGLLIGLLLNKPVNGKAFSSITMRVMDILLSFPSYLLAIAIVAFLGPGLEKGMIAI
                     GVVGIPVYARLARSAVLSVSQKEYVLASQSVGEGHGRILFKHVLPNILSPIIVQATMG
                     LASAILSAAALGFLGLGAIPPEPEWGAMLGDSYKFLSSGAWWAVLFPGLAIVFSVLGF
                     NLLGDGLRDALDPKMRTK"
     gene            4288..5304
                     /locus_tag="PKL60_06485"
     CDS             4288..5304
                     /locus_tag="PKL60_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95816.1"
                     /translation="METLMRIKDLHTRFHTQDGTVNAVNGISYAMQAGEVLGVVGESG
                     CGKSVHALSILRLIQIPPGEIEAGEIWFAGRDLLKLDDSGIRKVRGREIAMVFQDPMS
                     SLNPVYTVGFQISEALMLHKGMNKNQARERACELLNLVGIPEARKRLDNYPHQFSGGM
                     RQRAMIAMALSCEPKLLIADEPTTALDVTIQAQIIELVIRLQEELGMAVMWITHDLGI
                     VAEMAQNINVMYAGFLIERGNVHEIYHQTRHPYTLGLLGSLPSIDEAPGTRLISIPGL
                     PPDLIGLPQGCPFYARCAFRADHCRLERPELEEAGSSGHSVACWRWREIDAETLRKSA
                     GGAQ"
     gene            5301..6281
                     /locus_tag="PKL60_06490"
     CDS             5301..6281
                     /locus_tag="PKL60_06490"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNW95817.1"
                     /translation="MSAEQKSLVQVRNLKKYFPVSRQLFSTQRRFIKAVDDVSFDVLE
                     GETLGLVGETGCGKTTVARTILQLYRPTSGQVLFDGVDLASLQENDLRKMRSRMQMIF
                     QDPYASLNPRMTVGAIIAAPLDVHTRLDQKEKRDRVQELLKLVGLNPDFVNRYPHEFS
                     GGQRQRIGIARALALNPDLVICDEPISSLDVSIQAQVVNLLEELQNRLGLTYIFVAHD
                     LSMVRHISDRIIVMYLGKVMELADRNEIYLHPLHPYTRALMSAVPIPDPDKARNRKRI
                     ILKGDIPSPLNPPQGCNFNTRCPIASGRCFQEDPEYREISPEHWVACHFA"
     gene            6290..7342
                     /locus_tag="PKL60_06495"
     CDS             6290..7342
                     /locus_tag="PKL60_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNW95818.1"
                     /translation="MWNADIPAEGPCLSWQVVRLQLRTPFRLSYGATQERTAHWIRLR
                     GDAGWGEGTIPPYYPVREEDLQAYWNRAALRPEPFPERIEDIAAWIDPDGPATARAAL
                     DIAFHDSLSRKIGLPLNAALGLPKPEGKASSFTISVDTPENMAAEARRLSKLPILKLK
                     LAGDEGDIPRLEAVRAARPEARLFADANAGWSLEQALAYLPYLQKVGLEMLEQPLGAA
                     EIEGLGKIQAETSIAIVADESLQKPEDLRRLAKAGVRGVNIKLMKVGGVGPALELIRQ
                     ARTAGMKIMLGCMIETSIGLSAAAHLAGLADWLDLDASLLVANDPFEGMSFGEDAVIA
                     VPQRPGIGARVRGSAE"
     gene            7544..7906
                     /locus_tag="PKL60_06500"
     CDS             7544..7906
                     /locus_tag="PKL60_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95819.1"
                     /translation="MADFVEVKLFEVIPEKADAFEALCPEMTAWQRNCPGCQRMLLMK
                     REYTIDNPEKDPRKLKKIVKSVKYFAVWEFDTREDYAAACHAFFARYERPLGRVLIHP
                     FDIHLGYALTDAGWGTEP"
     gene            complement(7903..8733)
                     /locus_tag="PKL60_06505"
     CDS             complement(7903..8733)
                     /locus_tag="PKL60_06505"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HNW95820.1"
                     /translation="MIELPEAQTLAAQISQHLSGKTIINSTAAHSPHKFAWYYGDPAE
                     YPAKLNGGRVLSARGLGMFVEIALSTSTLLFNDGVNLRLHPQAGPIPVKHQLLLTFDD
                     GSSLSASVAMYGGVVCWANHELYENSYYATALAKPSPLTGAFDEACFRALLAPDAVRK
                     LPLKAALATEQRIPGLGNGCLQDILWQARLHPRKKLSTLTNEELHQLFLSLKSTFREM
                     AQAGGRSTEKDLFGQPGGYPVNLCAATNGKPCPRCGSPIMKEAYLGGSVYICPVCQKL
                     "
     gene            complement(8883..>9266)
                     /locus_tag="PKL60_06510"
     CDS             complement(8883..>9266)
                     /locus_tag="PKL60_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95821.1"
                     /translation="RTPEHREAVSRGDLCLATTYSPDTPFSVGTAMGRNKLIYTLADY
                     AIVVASDAEKGGTWAGATEALRLKWIPVFVLDHPDMPEGNKLLIQKGALQFPHPFPEH
                     YQNLKAWLEAQSTKIKPYSSQLSLF"
BASE COUNT         1922 a         2531 c         2781 g         2032 t
ORIGIN      
        1 ttgccggttt ccggtaaaaa accacatgaa aacgtagaaa atctcggtaa agactattga
       61 cgttttggtt tttcgtgtaa aattgattat tgttccccta tgcgttgaaa tggaggtcgc
      121 ctgccaacag atgacaaatc cgatccttga cccatattct tgttctgcaa attagttacg
      181 atgaggagaa agaaaatgaa acgcaagaca gtctttacag tgttcgggct tttgatagtc
      241 ttcagcatgc tcctttcggc gtgcgccaag acaggaacca aagccccagc ggctaccacc
      301 aaggatacgt tcatttttgg tcggggcgcc gattccgttc aattggatcc cagtatcgtg
      361 accgacggcg aatctttccg cgtgaccggg caggttctgg attccctgta caagtttgac
      421 aagggcacca cgaatccggt tcccgcgctg gcggaatgca ccgccaacga agacgcgacc
      481 gtctggagct gcaagctgct ggaaggcgtc aagttccatg atggcaccga tttcaacgcg
      541 gacgctgtca tcttcaactt cgaacgctgg cgctttacga cccatcccaa ccactatgcc
      601 agccaggttt tcgaatacta tgaagccatg tggggcggtt tcgacgatgc cagcatcatt
      661 accgaagtca agaagattga cgatcacaac gtgcaattca tcctgagcag cccgatggcg
      721 ccgttcctgg cgaaccttgc catggatatg ttcgcgatct ccagcccaac cgctattcag
      781 aaagccggag aagcctacgg cacgcctacc ggcggatgcg tgggcaccgg gccatacaag
      841 tttgtttcct gggaagaagg caccgagatt aagttggttg ccaatgaaga ttactggggc
      901 gaaaagccca aagtgaagaa catcatcgtc cgcgttatcc cggatgactc cgcgcgcttc
      961 ctggctctcc aggctggcga tattgacgcg ctggaacagg cggttgtgga agatctcgcc
     1021 accgctgaag cggatcccaa cctgcaaatc ctgacccgcc ccggtctgaa caccagctac
     1081 ctggctttca acttcaagat caaggaattc caggacgttc gcgttcgtga agctgtggcg
     1141 catgccatcg acatcgaggg cttaatcaag aacttcttcg gcaagtacgg caccgttgcg
     1201 aagaacttgc tgccggtggg catgctcggc tacaacgatt cgattgatta ctggacctac
     1261 gaccccgagc tttccaagag cctgcttgcc gatgccggct tcccgaatgg tctcagcgaa
     1321 gttacgattg ctgaagatgt tcaggacgcc gatggcaatg tggtctacac cgccgggcag
     1381 gttatcccgc tgcggctgta ctacatgccc gtgacccgct tctacttccc ggctgccaag
     1441 gaagttggcg aaggaattgc cgcgaacctg acggctgccg gaattaatgt ggaactctac
     1501 ctggaaggcg actggccgac ctacctcggc tcccgcagga acggcctgct gatgggtctg
     1561 tacctgctcg gctggggcgg cgataacggc gaccccgaca acttcacagg ttacttcttc
     1621 ggcagcggca cggaaccgat ccagcgcgaa ggctggtacc agaacgctga attggctgct
     1681 attctgcaga gagccgtaac gcttccggat cctgccgaac gcgcgaagct gtattctcag
     1741 gctgagcaaa tcatgcacga cgaggtgatg cgcatctggc tggggcataa caacacgccg
     1801 ctaattctct ccgccaaggt gaaggattac attccgcagc ctgtcggcgc cgagaacttc
     1861 gagtacgtgt atttcagcga ctaaccatta atgcgggcct gattggtctg cagctaagca
     1921 tccatatggg ggcggttccc aaaccgcccc ctttcacaaa atacaagcga ggtcagcttg
     1981 gcaaagtata ttttgcgaag gttaatctcc gtcatcccga ccctgatagg cgtgaccctg
     2041 gttattttct tgtttcagcg cttaatcccg ggggatccgg ccgtcgccat gcttggcgaa
     2101 cacgctaccg aagaaaacgt ggccagaatt cgggaacaat tcgggttgaa ccgccctgcc
     2161 tttctggacc gggatgcgct ggtggaaggc gacctgcctg gtttctttga cagtcagtac
     2221 gtgcgctatt ttaatcgtct gctgcacttt gacctggggg actccattca ccgccggatt
     2281 ccagtcgccg aaacgctcaa ggaacgcttc ccggccacgc tggagcttgc cttgctctcg
     2341 atgtttttcg cgctcctgat cggtattccg gtcgggatca cctccgcctc ccgccgcaac
     2401 tcctggctgg atgggacgac catgataggt tcccttctgg gggtttccat ccccattttc
     2461 tggttgggca tcatggagat tatgttattc tcggtatttt tgaagtggct tccatctgga
     2521 agccggctaa gccccggcat gagtatcacg cctatcacaa acctgctcct gctcgacagc
     2581 ctgctgaccg gtaacttcgc cggcttttgg gacgcgctca agcatattat tatgccagcg
     2641 gtttccctgg caaccattcc gaccgccatt atcacgcgta tgacgcgctc cagcatgctg
     2701 gacgtccttc aggaagacta tatccgcacc gcctcggcaa agggccttac ggaacgggta
     2761 gtgctttatc ggcacgcgct caagaatgca tttctgccgg tcatcacaat tatcggcatt
     2821 caggcaggct ctttgctggc tggagccgtg ctgaccgaaa cgattttttc ctggccaggc
     2881 atcggcaaat gggtctatga ctccatattg ggtcgggatt acccgattgt gcagggcgta
     2941 acgcttctga ttgctgttat tttcctggtt gtcaatttat tggtcgacat ttcctacgcg
     3001 cttctcgacc cgcgtatcag ttacgattag gattgactat gagcacgcaa atgcctgaag
     3061 ttgaagtcct gaccgatttt gctgtgcgtg agcaccgctc cctttggctg gatgcctggc
     3121 gccgcatgaa agccagccgg actgccatgc ttgggttggt ggtcgtggtg gtgtttctgt
     3181 tatcagccgt cggcgcgcat ttattctgga attatgatgc caaaactgac ctcgattaca
     3241 gcgcaaaact cttgccgccc aatctggtca gcagcacgga aaacccaaac gtgcacattt
     3301 tcgggaccga taagcttggc cgggatattt tcacccgcgt gattcacggc ggttggaatt
     3361 ccctgcgcgt agggctggtg gcggtgggta tctctctgat tatcggctgc ctgcttggtt
     3421 tgttttccgg gttctttgag agctttgaga tgaatcgcat agaaaagctg gtcctgatga
     3481 gcgggctggg cctggcttta gggcttttgc ccgcctggat tgccagccaa tggtaccaaa
     3541 ccctcctgtt cggagggctc ggcgcggcag cggtgtttgt ggagaagaag cttgcgcaga
     3601 gtaagttgcg cccggtcttg ttcgctgctg cgggagcgct gttaggcggg ctcagcggac
     3661 tgctgctcgc gccgttggtg ggactggtgt gcggggtggt tgggctgctc atcggtctgc
     3721 tgctgaacaa acccgtgaat gggaaggctt tctcgagcat caccatgcgc gtgatggata
     3781 ttctgctttc cttcccgagc tacctgctgg cgattgccat tgtggcgttc cttgggcctg
     3841 ggctggagaa aggcatgatt gccattggcg tggtcggcat tccggtctac gcccgcctgg
     3901 cgcgttcggc ggtgctttcc gtcagtcaga aggaatacgt gctggcttcg caatcggtag
     3961 gcgaggggca cggacgcatc cttttcaagc acgtgctgcc caatatcctc tcgccgatta
     4021 tcgtccaggc gacgatgggc ctggcttcag ccatcctttc cgcggcggcg ttgggcttcc
     4081 tcggtctggg cgccatccca ccggagccgg aatggggcgc gatgctgggg gacagctaca
     4141 agttcctctc cagcggcgcc tggtgggcgg tgctcttccc cggcctggcg attgtgttca
     4201 gcgtgctggg gttcaacctg ttaggcgacg gtctgcgcga cgcgttggac ccgaaaatgc
     4261 gcaccaagtg atgggggagg ggatgctatg gaaactttaa tgcgaatcaa agacctgcac
     4321 acccgttttc acacccagga cggtacggtt aacgcggtca acgggatttc ttatgccatg
     4381 caagccggcg aagttctggg cgtggtcggc gaaagcggct gcggcaagag cgtgcacgcg
     4441 ctttcaatcc tgcggctcat ccaaattccg cccggcgaga ttgaggccgg ggaaatctgg
     4501 tttgcaggca gggacctgct caaactggac gacagcggga ttcgcaaggt gcgcgggcgc
     4561 gaaattgcca tggtctttca ggacccgatg tcctcgctga atccagtcta caccgtgggg
     4621 tttcagattt ccgaagcgct tatgctgcac aagggcatga ataagaatca agcccgcgag
     4681 cgcgcatgcg aactcctcaa cttggtgggc atcccggagg cgcgcaaacg cctggacaac
     4741 tatccccacc aattttcggg cggcatgcgt cagcgcgcca tgattgcgat ggcgctttcc
     4801 tgcgagccca aactgctcat cgcggacgaa cccactaccg cgctggatgt cacaattcag
     4861 gcacagatta tcgagctggt gattcgcctg caggaagaat tgggcatggc ggtgatgtgg
     4921 attacgcatg acctggggat tgtggcggag atggcgcaaa acatcaacgt catgtacgcc
     4981 ggcttcctta tcgagcgcgg caatgtgcac gagatttacc atcaaacacg ccacccctac
     5041 acgctgggat tgcttggctc gctgcccagc atcgatgaag ctccgggcac ccgattaatc
     5101 tccattcccg gcctgcctcc ggacctgatt ggcttaccgc aaggctgccc tttctatgcg
     5161 cgctgcgcgt tccgggcgga tcactgcagg ctggaaaggc ctgaactgga agaagctggc
     5221 agctccgggc atagcgtggc gtgctggcgc tggcgggaaa tcgacgcgga gacgctgcgg
     5281 aaatctgccg gaggtgcaca atgagcgctg aacaaaaaag cctggttcag gtgcgaaacc
     5341 tgaaaaagta cttccccgtt tcacgccagc tcttcagcac ccaacggcgc ttcatcaaag
     5401 ctgtggacga tgtgagcttt gacgtcctgg aaggcgaaac cctcgggctc gtcggggaga
     5461 ccggctgcgg aaaaacgacc gtggcgcgca cgattttgca gctctaccgc ccaacctccg
     5521 ggcaggtgct gtttgatggg gtggatctgg ccagtctgca ggaaaacgac ctgcgcaaaa
     5581 tgcgcagccg catgcagatg atttttcaag acccctacgc ctcgctcaac ccgcgcatga
     5641 ccgtgggagc tattatcgcc gctccgctgg atgtgcatac caggctggac cagaaggaaa
     5701 agcgggatcg cgtgcaggaa ctgctcaaac tggtcggtct gaacccggat ttcgtcaacc
     5761 gctatccgca cgaattttct ggcggtcagc gccaacgcat cggcatcgcg cgcgccttgg
     5821 cgctcaaccc cgacctggtc atctgcgatg agccgatttc ctcgctggac gtcagcatcc
     5881 aggcgcaggt ggtcaacttg ctggaggaat tgcagaaccg gctgggctta acctacattt
     5941 ttgtggcgca tgatttgagt atggtgcggc acatcagcga ccggattatc gtgatgtacc
     6001 tgggtaaggt gatggagctg gcggaccgga acgaaatcta tctgcatccc cttcatccat
     6061 atacgcgcgc gctaatgtcc gctgtaccga tcccggaccc ggataaagcc cgcaaccgca
     6121 agcgaatcat cctcaaaggc gacatcccca gccccctgaa tccaccgcag ggatgcaact
     6181 ttaacacacg ctgtccgatt gcctcagggc gatgcttcca ggaagatcct gagtaccgcg
     6241 aaatcagccc cgaacattgg gtggcttgcc actttgcatg aggtaagctg tgtggaatgc
     6301 tgacattccc gcggaaggtc cctgcctgag ctggcaagtc gtcaggctgc agctgcgcac
     6361 cccgttcagg ctttcctacg gcgccacgca ggaacgcact gcccattgga tccggctgcg
     6421 cggagacgcg ggttgggggg agggcaccat cccgccttat tatccggtgc gggaagagga
     6481 tttacaggcg tactggaacc gcgccgcttt gcgcccggaa ccattcccgg agaggataga
     6541 ggacatcgcc gcatggatag accctgacgg gccggctacc gcccgcgccg cgctggatat
     6601 cgctttccat gattccctca gccgcaagat tgggctgccg ctcaacgccg cgcttgggct
     6661 gcccaaaccc gaggggaaag ccagcagctt caccatttcg gtggatacgc cggaaaacat
     6721 ggcggctgaa gcccggcgcc tgagcaagct gccgattctg aagttgaagc tggcaggcga
     6781 tgaaggggat atcccgcgcc tggaagccgt gcgcgcggcc aggcccgaag cgcgcctgtt
     6841 cgcggacgcc aatgccggct ggagcctgga gcaggcgctt gcgtatctgc cgtacttgca
     6901 gaaagtgggg ctggaaatgc tcgagcagcc gctgggcgct gcggagattg agggtttggg
     6961 gaaaatccag gcagaaactt ccatcgcgat tgtggcggat gaatcccttc agaagccgga
     7021 agacctgcgc cgtttggcaa aggccggcgt gcgcggggtg aatatcaaac tgatgaaagt
     7081 cggcggggtc ggccccgcgc tggaattgat caggcaggca cgcactgccg gaatgaagat
     7141 tatgctgggc tgcatgattg agacctcaat cgggctaagc gccgcggcgc atttggccgg
     7201 gctggctgat tggctggacc tggatgccag cctgctggtg gcgaatgatc cattcgaggg
     7261 aatgagcttc ggggaggatg ctgtcatcgc agtgccgcag cgccctggga ttggcgcgcg
     7321 agtgcgcggt tccgcggaat aagggcgcga gccttcctga gcgggtaaaa actccggtga
     7381 gagctcaaga tgattcctgg agattcggaa gagcaataga taagctgaca aagcccggaa
     7441 taaagcaaag gtcaggctat ttttgtgcgg gcagggggag gagcatggac ttctcgacct
     7501 gacaggctta tagtaagagc aaaagtgaga tcagagtgca ggaatggctg attttgttga
     7561 ggtaaaactt tttgaagtga tcccggagaa ggcggacgcg tttgaggcgc tctgcccgga
     7621 aatgacggcg tggcagcgga actgcccagg ctgccagcgc atgttgttga tgaagcgcga
     7681 gtacacgatt gataacccgg agaaagaccc gcgtaagctg aagaaaatcg ttaaatcggt
     7741 caagtatttc gcggtttggg agtttgacac gcgcgaggat tacgctgcgg cgtgccacgc
     7801 gttcttcgcg cggtacgaac gcccgcttgg aagagtactc atccatccct tcgatatcca
     7861 cctgggctac gcgctgacag acgccggctg gggaacggaa ccctaaagct tctggcaaac
     7921 ggggcagatg tagacgctgc cgcccaggta ggcttctttc atgattgggg agccgcagcg
     7981 ggggcagggc ttgccgttcg tcgccgcgca taaattcacc ggatagccgc cgggctgccc
     8041 aaagaggtcc ttttctgtgc tgcggccgcc ggcttgggcc atttccctaa aggttgattt
     8101 caagctaaga aaaagttgat gcagttcctc attcgtgaga gtgctgagct ttttacgcgg
     8161 gtgcaggcgc gcctgccaga ggatatcctg caagcagccg ttaccgagcc ccgggatgcg
     8221 ctgctcggtg gccagggcgg ctttcagcgg cagcttacgg acagcatcgg gcgcgagcag
     8281 cgcgcggaag caggcttcgt cgaaagcccc ggtcagcggc gagggctttg ccagggcggt
     8341 cgcgtaataa ctgttctcgt agagctcgtg atttgcccag cagactacgc cgccgtacat
     8401 cgccacggaa gcgctcaggc tggagccgtc gtcaaacgtg agcaggagct gatgcttgac
     8461 agggatgggg ccggcttgtg ggtgcaggcg caggttcacg ccgtcgttga aaaggagggt
     8521 gctggtgctg agcgcgatct ccacgaacat gcccagcccg cgggcggaga ggacgcggcc
     8581 gccgttcagc ttggcggggt attccgccgg atcgccgtaa taccaggcga atttgtgcgg
     8641 gctgtgggcg gcggtggagt tgattatagt tttaccgcta agatgttggg agatttgagc
     8701 ggcgagggtt tgggcttcag gaagttcaat catgggcggc cttgggatga gtggtatgcg
     8761 tcggaaattc gagtatccgg cagcggaaaa cgtttttgat gaattggtag gctggccagt
     8821 agatcagcaa aacatgcaga catgattagc tcttttctga gcggaaacag aaggttgaaa
     8881 tatcaaaaaa gtgatagttg gctggaataa ggtttgattt ttgttgattg cgcctccaac
     8941 caggctttta agttctggta atgttctggg aaaggatggg gaaactgcaa ggcacctttt
     9001 tgaatgagta gtttgtttcc ttctggcata tcgggatgat ccagcacaaa caccggaatc
     9061 catttcaatc tgagcgcctc ggtcgcgccg gcccaggtac cgcccttttc cgcgtcactt
     9121 gccacaacga tggcgtaatc tgcaagggtg taaatcaatt tattcctacc cattgctgtt
     9181 ccgacagaaa acggagtatc cggagaatat gtggtcgcca gacataaatc accacgactt
     9241 actgcctcac gatgttctgg agtccg
//
LOCUS       DAOQAD010000073         9102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_9016_length_9102_cov_5.705869, whole genome shotgun sequence.
ACCESSION   DAOQAD010000073 DAOQAD010000000
VERSION     DAOQAD010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9102
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9016_length_9102_cov_5.705869"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..704
                     /gene="rnpB"
                     /locus_tag="PKL60_06515"
     ncRNA           343..704
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKL60_06515"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            730..1689
                     /locus_tag="PKL60_06520"
     CDS             730..1689
                     /locus_tag="PKL60_06520"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNW95822.1"
                     /translation="MNFQRAANTQKRFPALILWIAAVGLLLGACSSQPDNLVIIVIPS
                     TGTILPLHQETQIISQVIARRGWSRLELYANGELVRVDSATADTMYSAILKQPWIPTA
                     EGPVLISVIAVDARERRIASAEAAVLIGSPSPTALSASPTPSATPTPAPLLSPTPCTP
                     QAALLQDVSIPPGTLLEPGQSFTKTWRVQNTGTCAWSGYQLVFISGSLLGGKSPTRVS
                     DLAPGAAVNLSLELSAPRYQGDYAGTWRLQTDQGVIFGPELSFSIRIPQPTPTRTLTP
                     TASATWTPTLTPTPTPTPTNTPTPSPSPTPSGTVTPTPPSSEP"
     gene            complement(1968..2537)
                     /locus_tag="PKL60_06525"
     CDS             complement(1968..2537)
                     /locus_tag="PKL60_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNW95823.1"
                     /translation="MFRDLVLKSRSYRRFDESVRIPRETLVELVDLARHAPCSANLQG
                     LKFSLITEKAQTDHVFPLVKFAGYLKNWGGPAEGERPAAYIVVLGDERIKRQYDLDTG
                     IAAQTMMLGAVEKGFGGCMMSSVKREDLAALLELPAYLEIVLVLALGKPAEMVVIEPV
                     GADGDLKYYRDEQGVHHVPKRALEDLIYD"
     gene            complement(2530..2985)
                     /locus_tag="PKL60_06530"
     CDS             complement(2530..2985)
                     /locus_tag="PKL60_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95824.1"
                     /translation="MKSNPESKDRTRNWIARALIAVVLTLNLQAALQYMITPQAFSAG
                     FELSGTPGATAVAGVGILYLMWQVPYVFALTNPISHKISLIEALLMQAIGLLGESWLL
                     STIDPNHAALRSSIVRYIIFDAGGLVLLACAYGAAHCRRLLFKKGETHV"
     gene            complement(3004..4209)
                     /locus_tag="PKL60_06535"
     CDS             complement(3004..4209)
                     /locus_tag="PKL60_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNW95825.1"
                     /translation="MHLTRLALTNFRIFSRLDLQLPRKVILLAGSNAQGKTTILEAAA
                     YLANFSSFYAATDRQLLNFNLQPEPVLVGRIVGEYQTRLKTHTLEVRFIQEYNGNPNA
                     PRFRKEVLLDGVKKKLGEVYGQFNAVSFLPQMSRILEGSPSDRRQYLDEALSQAQQGY
                     ARHCADYAKALNQRNALLKILSERGGDTRQLEAWDEMLASHGAELMHARIHLLKELEE
                     QAGPIHQQLTRGTEILRLQYKPSYEPLPAPKGQLSLPVQTAVDRSPLSALEIEAGLRK
                     ALHAAYREDISRGMTTLGPHRDEFRFIGSGIDLGDYGSRGQARTALLSLKFAEVQWMR
                     TKTGEWPVLLLDEIMAELDPERRADLLAVLTQVEQALLTSTDPDMFPPEFTHNHEIWH
                     VRDGMVYQN"
     gene            complement(4209..4718)
                     /gene="def"
                     /locus_tag="PKL60_06540"
     CDS             complement(4209..4718)
                     /gene="def"
                     /locus_tag="PKL60_06540"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNW95826.1"
                     /translation="MAVREIVKFPVPSLRLKAKPVQVFDKELQRLIDDMFETMQAAPG
                     VGLAAPQIGESLRVVVVEYTDDEGENAKPKKYVLVNPEFVHKSEETVTDVEACLSVPG
                     LAGEVERHEAVTIRAKNRFGKPIRLKASGWLARIFQHEIDHLDGVLYIDRATQVFQPT
                     AEELEQVKD"
     gene            complement(4756..6420)
                     /locus_tag="PKL60_06545"
     CDS             complement(4756..6420)
                     /locus_tag="PKL60_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNW95827.1"
                     /translation="MPAENTALKELLYAHPFFSLVDEELLRALSSGAELITLEAGSLL
                     YSAGTNANAAFLLAAGKIERGDPENPAENRIILEGELAGDEPLDGLENYTCSARTLTD
                     CSIWQIPAQQLREALLSSPRALRARAVLWQSRRLAERIRMSWLEKDEQALFITRKHPF
                     FLLMSFLLPVLGFAVLVAAGVLFGAARPDLTVFAMAAGFVFCALWLAWNHHNWANDYY
                     IITNHRMVWVEKVSGFYESRQEAPLGMVTSVGVQSTQPGRILGYADVVVRTIIGNLRF
                     NRVADSDVIEHLVEVFWRRRQSEQAAFEEEKMRAALRQKLAGTGATQPVQPKEGESLP
                     LESDLPLLREKSFFAWLFGDFLKVRYEIGGVTTYRKHWFVLLGKALLPTLVLACGIVL
                     LAATLTWNFKLLPYNLSVFLGVLFVIAALIWLVYNYADWRNDIYRLTPDQVIDIDRKP
                     LGRESRRTAPLEKILAVEYERRGLIPMLLNFGTVFIRVSTEVLTFDNVYQPSKVQEDI
                     FRRMQAAAAAARERDIDEERERVARWFSVYHAQTQDTTKRLNPPPL"
     gene            complement(6518..8986)
                     /locus_tag="PKL60_06550"
     CDS             complement(6518..8986)
                     /locus_tag="PKL60_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW95828.1"
                     /translation="MMRFDKFSERAQDVAARAAEIIQRYGHTQIDTEHIMLALIEQPE
                     GMVSQILDMLKLDAGQLADSIDHLLRTTPKANIFGGASGQIFMTPRVKLAVDLAKNEA
                     SRFGDEYISTEHLFLAILAERNTALARILENHGVIREKVMDAILEIRGGSKRSETHSE
                     TQYKTLDKFSRNLTQAAREGKLDPIYGREEEISRLIQILCRRTKNNPVLIGEAGVGKT
                     AIVEGLAQKIANNDVPEILEDKRVVALDLGAMIAGTRFRGEFEERLKAAIDEIQKSNG
                     EVILFIDELHTVVGAGAAQGAMDASNMLKPALARGDLQCIGATTLDEYHKFIEKDAAL
                     ERRFAPIFVEEPSIDDTILMLRSLRDRYEAHHKVTISDEALSAAAKLSSRYMTERKLP
                     DKAIDLMDEAAAKLRVALYSMPEALRDQKREIERLAAEEEQASVERDYERAVKMKADR
                     LRLEQEFNAQREKWEVEQKLDEVVDVNDIADVIHQWTGIPLTQLMESEAQKLLHMEDR
                     LHERIIGQDEAIKAISDAIRRARSGMKDPRRPSGSFIFIGPSGVGKTELAKALAEFLF
                     DDEDALVRIDMSEYREQHTVSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVVLFDEIEK
                     AHPEVWNALLQILDDGRLTDGQGHIVDFRNTVLIMTSNLGTEFVKKGGALGFVDSNDG
                     EDIKSSHEKIQKALKSAFRPEFLNRIDDIIMFSPLTHEDVMGIVTLQLAELQGRLDEY
                     GVKMEVSSAARSWLADQGYDPLMGARPLKRAMQKFLESPLATQLLASGFQAGDVVLVD
                     KDPEGGLTFAKKEAANPAAAPLEA"
BASE COUNT         1835 a         2813 c         2456 g         1998 t
ORIGIN      
        1 aacatatcga acttaacaac tgcgaatcgc aagaagtaga gccaatgatt atggtgactg
       61 cgtcggaaca aaacgacagg agaagacccc ctacctgccc gggtcaggaa accacgatta
      121 acgaaaaatc gttatccact gtgagagata catgggggta tggcttcagc cgtaaatatg
      181 ctctccgcct tatctcacgt cgggctgcat ccttcgaccc gacatttcag gaggctagaa
      241 aagcgcatgc tgccctgcag cgtcccgtct gaaacccggg ttcccacgcc aagtctggct
      301 ctgattgact tttccccccc tttccgctaa cataatattg ctcaggtcag tcgggtgtgc
      361 gcgccgccgt tcgcggcggt gaggaaagtc cgtacacctc agagcaggaa gccaggtaat
      421 ccctggagag gcgcaagctt ctggatcggg ccacagaaac ataccgcacg cgcgagcgtg
      481 tcagggtgaa aaggcggtgt aagagaccac cggcgcgggc agtaatgccg gcggccaggc
      541 aacccccttc cggtgcaagg tcaaacaggg gaaaagcatc ctcggatgcc agcgctggct
      601 cggcgctgtt gatccccggg tagaccgcat gagcagtctg gtgacagact gtcgagatag
      661 atgcgcatcc gcgacagaat acggcttaca ggctgacctg agtgccttgc ccgccttcct
      721 gaggaagtta tgaattttca acgcgccgcg aacactcaaa aacgatttcc cgcccttatc
      781 ctctggattg ccgctgttgg cttgctgctc ggcgcctgtt cctctcaacc ggataacctg
      841 gtcataatcg tcattccctc caccggcacg atccttccgc tgcatcagga gacgcaaatc
      901 atctctcagg tcatcgcccg ccgcggctgg tcccgacttg agttgtacgc aaatggtgag
      961 ttggtgcgcg tggatagcgc caccgcagat accatgtact cagcaatcct caaacagccc
     1021 tggatcccga cggcggaagg acccgtcctc atcagcgtga ttgcggtcga cgcgcgcgaa
     1081 aggcgcattg ccagcgcgga agcagctgtt ctaatcggtt cacccagccc gacggccctg
     1141 tccgccagcc ctaccccaag cgctacgccc acccccgcgc cgctgctcag cccaacgccg
     1201 tgcacccccc aggcggcgct gctgcaagac gtcagcatcc ctccgggtac gctgctggag
     1261 cctgggcagt cctttaccaa gacctggcgg gtgcagaaca ccggcacctg cgcctggagc
     1321 ggatatcagc tggtcttcat cagcggcagc ctgctcggag gcaaatctcc aacccgtgtc
     1381 agcgacctgg cccccggcgc ggcagtcaat ctctcgctgg aactcagcgc cccgcgttat
     1441 cagggcgact acgcgggcac atggcggctg caaacggatc aaggcgttat ttttggcccc
     1501 gaactcagtt tcagcatccg catcccgcag cctaccccca cccgcacgct taccccgacg
     1561 gcttccgcca cctggacgcc aacgctgacg cctacgccca ccccgacccc gaccaacact
     1621 cctacgcctt cccccagccc cacgcccagc ggaaccgtca cgcccacccc gcccagctcg
     1681 gagccgtaag ttccgtcagc cctccgaaga aggaaacgaa atgggctgtc cacaagccgg
     1741 acggcccttt ttgatttccc gctccgtgcc cgtttttatg gtcgtttgcg gatttgcgct
     1801 atcaaaacga aggctaagcc aggcacgttc tcccactttg ggctgtccaa aaaggaaaac
     1861 cctttctttt tggacaacct gccttttcgg catgcaagat acctgcttat ccagctcagc
     1921 cgcgccgcct gccgcccggg aaagtcccgg gagtgtggaa attcctctta atcgtaaatc
     1981 aggtcttcga gcgcgcgctt gggcacatgg tggacgccct gttcatcccg gtaatacttg
     2041 agatcgccat cagcgcccac gggttcaatc acgaccatct ccgcgggttt tcccagcgcc
     2101 aacaccagca caatctccag gtaagcgggc agttccagca aggccgccag gtcctcacgc
     2161 ttcacggagc tcatcatgca accgccaaaa cctttctcga ccgcgcccag catcatcgtt
     2221 tgcgcggcaa tgccggtgtc caggtcatac tgccgtttta tgcgttcgtc ccccagcacc
     2281 acaatgtagg ctgccggcct ttccccctca gcgggtccgc cccaattctt caggtagccg
     2341 gcaaacttaa ccagcggaaa gacatggtca gtctgggctt tctctgtaat caggctgaat
     2401 ttgagcccct gcaaattggc ggaacaggga gcgtgcctcg ccaggtcaac caattccacc
     2461 agcgtctcgc gcgggatgcg gacggactcg tcaaagcggc ggtaactgcg gcttttcaga
     2521 accaaatctc taaacatggg tttcaccttt cttgaataag agacggcggc agtgcgccgc
     2581 gccatatgcg caggcgagca gtactaatcc ccccgcgtcg aagataatgt aacgcacgat
     2641 gcttgagcgc agcgctgcat ggttcgggtc aatcgtgctc aagagccagc tttcacccag
     2701 taagccgata gcctgcatca ggagcgcctc aatcagggaa atcttgtgtg aaatcgggtt
     2761 ggtcagggca aagacgtagg gcacctgcca catgaggtac agaatcccga cacccgccac
     2821 cgcagtcgca cccggagtgc cgctcagctc aaaccctgcg ctgaacgcct gcggggttat
     2881 catatattgc aaggctgcct gtaagtttag cgtcagcacc actgcaatca gcgcccgggc
     2941 aatccaattc ctcgtcctat cctttgattc cgggttcgat ttcatcttgt gcctgccttg
     3001 ggtttagttc tggtaaacca tgccgtcgcg cacatgccaa atttcatggt tgtgcgtgaa
     3061 ttcaggcggg aacatgtccg ggtcggtcga agtcaaaaga gcctgttcca cctgagtaag
     3121 gacagccagc aaatccgcgc gccgttcagg gtccaattcc gccataattt catcgagcag
     3181 aagcaccggc cattcgccag tcttggtccg catccactgc acttccgcga acttgagcga
     3241 gagcagcgcg gtgcgcgcct gccccctgga gccgtaatcg cccaggtcga ttccgctgcc
     3301 aatgaagcgg aactcatccc ggtgcggacc cagcgtggtc atgccgcggc tgatatcctc
     3361 gcggtaagcc gcgtgcagcg ctttgcgcaa gccggcctca atctccagcg cgctgagcgg
     3421 cgaacggtcc acagcggtct gcaccggcag agagagctga cctttgggcg cgggtaaggg
     3481 ttcgtaagaa ggcttgtact gcagccgtaa tatttctgta ccgcgggtaa gctgctggtg
     3541 aatcggacca gcctgttctt ccagctcctt cagcaagtgg atgcgcgcgt gcatcagctc
     3601 cgcgccgtgg ctcgccagca tttcatccca ggcttccaac tggcgggtgt cgccgccgcg
     3661 ttccgaaaga atcttgagca gggcgttgcg ctggttcaag gccttggcgt aatccgcgca
     3721 gtggcgggca taaccttgct gcgcttgcga aagcgcttcg tccaggtact gccgccggtc
     3781 ggagggcgag ccctccagga tgcgcgacat ctgcggcaga aagctgaccg cgttgaattg
     3841 cccatacact tcgcccagct tctttttcac gccgtccagc agcacttctt tgcggaacct
     3901 cggcgcgttg ggattgccgt tgtattcctg gatgaagcgg acttcgagag tatgggtttt
     3961 tagtctggtc tgatattcgc ccacgatgcg gcccaccaaa acaggctcgg gctgcagatt
     4021 gaaattcagg agctgccggt cagtcgcggc ataaaaagag gagaaattcg ccaggtaggc
     4081 tgctgcttcg aggattgtcg tttttccctg ggcattcgag ccagccaaaa gaatgacctt
     4141 ccggggaagc tgtaagtcca gccgggaaaa aatcctgaaa ttagtcagcg cgagccgggt
     4201 gagatgcatt aatccttgac ttgctccagc tcttccgctg tgggctggaa cacctgcgtc
     4261 gcgcggtcaa tgtagagcac gccgtccagg tggtcaatct catgctggaa aatgcgcgcc
     4321 agccatccgc tggctttcag gcggatgggc ttcccaaagc ggttcttcgc ccggatggtt
     4381 actgcctcat ggcgttcaac ctcacctgcc agacccggca cggagaggca ggcctccaca
     4441 tccgtgacgg tttcctcgga tttatgcaca aattccgggt taaccagaac atattttttg
     4501 ggcttcgcgt tctcgccttc gtcgtccgtg tattccacga ccaccacgcg cagggactcg
     4561 ccaatttgcg gagcggccaa accgacgccc ggtgcagcct gcatggtctc gaacatgtcg
     4621 tcgatcagtc tttgcagctc tttatcaaaa acctggacag gtttcgcctt caaccgaagt
     4681 gaaggcacag ggaattttac aatctcgcgt acagccactt tatctccatt cgaattcttt
     4741 cggactaatt ctaccttaga gaggtggcgg gttcaatctt tttgtagtgt cttgggtttg
     4801 ggcgtggtaa acgctgaacc agcgcgcgac gcgctcgcgt tcctcgtcga tatctcgttc
     4861 gcgcgcggcg gcagccgcgg cctgcatcct gcggaagata tcttcctgaa cttttgaggg
     4921 ttggtacacg ttgtcaaaag tcagcacttc ggtggacacc cggatgaaga cggtgccaaa
     4981 attcagcagc atcgggatga gcccccggcg ttcgtattcc actgccagga ttttttccag
     5041 cggcgcggtg cgcctggatt cacgcccaag cggtttccgg tcaatatcaa tcacctggtc
     5101 gggggttagg cggtaaatat cgttgcgcca gtctgcgtag ttgtacacca gccagatgag
     5161 cgcggcaatc acgaacagca cgcccagaaa aaccgacaga ttgtacggca gcagcttgaa
     5221 attccaggtg agtgtagctg ccaagagcac aattccacat gccaaaacaa gcgtcggcag
     5281 cagcgccttc cccagcagca cgaaccagtg cttgcggtac gtggtcaccc cgcctatctc
     5341 gtagcgcacc ttcaggaaat ccccgaacaa ccaggcaaaa aagcttttct cgcgcaagag
     5401 cggcagatca ctttccagag gaaggctttc cccttccttg ggttgaaccg gctgtgtggc
     5461 tccagttcct gccagctttt ggcgcagcgc cgcccgcatt ttttcttctt caaaggctgc
     5521 ctgttcagac tgtctgcgcc gccagaacac ttccaccaga tgctcaatca catccgagtc
     5581 tgccacccgg ttgaaacgca agttgccgat aattgtacgc accaccacat ccgcgtatcc
     5641 caggattctg cccggctgag tgctttgcac cccaaccgaa gtcaccattc ccaggggtgc
     5701 ttcctggcgc gactcgtaga accccgatac tttttccacc caaaccatcc ggtggttagt
     5761 gatgatatag taatcattag cccagttgtg atggttccaa gccagccaga gcgcgcagaa
     5821 gacaaaaccc gccgccatgg caaacaccgt caagtccggt ctggctgccc caaacaaaac
     5881 cccggccgcc accagcacgg caaaacccag cacaggcagc agaaagctca tcagcagaaa
     5941 gaatggatgc ttgcgggtga tgaaaagcgc ctgctcatct ttttcgagcc agctcatgcg
     6001 gattctttcg gccaggcgcc ggctttgcca cagcaccgcg cgggcgcgta gcgccctggg
     6061 agagctgagg agcgcttcgc gcagctgctg cgcgggaatt tgccagatgg agcagtcggt
     6121 cagcgttcgg gcgctgcagg tgtagttttc cagcccgtcc aaaggttcgt cgcctgccag
     6181 ttccccttcc agaataatcc ggttctcagc agggttttcc gggtcaccgc gttcaatttt
     6241 ccccgcggcc aggagaaaag cggcattcgc gtttgtgccg gcgctgtaca gtaagcttcc
     6301 cgcttccaat gtgataagct ccgccccgga agaaagcgcg cgcagcagct cctcatccac
     6361 gaggctgaag aacggatgcg cgtacagcaa ttctttcaat gcggtgtttt cagcgggcat
     6421 tttcgattat ccacgcagca gtctcatcca cagccctgcc gcattttctg aaattcggct
     6481 gcccgctttc acgggcagcc ttctcatcta tcgttattta cgcctcaagc ggagcagccg
     6541 cagggttagc cgcttccttt ttagcaaagg tcagccctcc ttcggggtct ttatccacaa
     6601 ggacgacgtc ccccgcttga aaaccgctgg ccaggagttg ggttgccaat gggctttcca
     6661 ggaatttctg catcgccctt ttcagcggtc tcgcgcccat gagcgggtca taaccttggt
     6721 ccgccagcca cgagcgcgcg gcgctgctca cttccatctt cacgccgtat tcatccaggc
     6781 ggccctggag ctccgccaac tgcagggtga cgattcccat cacgtcttcg tgcgtcagcg
     6841 gcgagaacat gatgatgtca tcgatgcggt tcaggaattc cggccggaag gcgctcttca
     6901 aggccttttg aatcttctcg tgcgacgatt tgatatcctc gccgtcgttg gaatccacga
     6961 aacccaacgc gccgcccttt ttcacaaact cggtgcccag gttgctggtc ataatcaaca
     7021 ccgtgttgcg gaagtccacg atgtgcccct gtccatcggt caggcggccg tcgtccagta
     7081 tctgcagcag cgcgttccag acttccggat gggctttctc gatttcatcg aagagcacca
     7141 cgcggtatgg tctgcgccgc acagcttcgg tcaattgccc gccttcttcg tatccgacgt
     7201 agccgggagg cgcgccgaac aagcgcgaaa cggtgtgttg ttcgcggtat tcactcatat
     7261 ctatgcgcac caaagcgtct tcatcgtcaa acaggaattc cgccagcgcc ttagccagtt
     7321 cagtctttcc cacgccggaa ggcccgatga agataaacga gccgcttggc cggcgcgggt
     7381 ctttcatgcc cgagcgcgcc cgccggatgg catccgaaat tgccttgatt gcttcgtcct
     7441 gtccgatgat acgttcgtgc agacggtctt ccatgtgcag cagcttctgg gcttcgcttt
     7501 ccatcagttg ggtcagcgga atgcctgtcc attggtggat gacgtcggca atgtcgttca
     7561 cgtccaccac ttcatccagt ttttgttcga cctcccactt ttcgcgctgg gcattgaact
     7621 cctgctccag gcgcagccgg tcggctttca ttttcaccgc gcgttcatag tcgcgttcca
     7681 cgctggcctg ctcttcctcc gccgccaggc gttctatctc ccgcttctgg tcgcgcagcg
     7741 cctcaggcat ggagtacaag gcgacgcgca gcttggcagc ggcttcgtcc atcaagtcga
     7801 tggccttatc cggcagtttc cgttcagtca tgtaacggga cgagagcttg gccgcggcgc
     7861 tgagggcttc atctgagatt gtcaccttat ggtgcgcctc atagcggtcg cgcagactgc
     7921 gcagcatcag gatggtatcg tcgatgctgg gctcctccac aaaaatcggc gcaaagcggc
     7981 gttccagcgc ggcatccttc tcgatgaact tatggtattc atccagggtg gtcgcgccga
     8041 tgcactgcag gtcgccccgt gccagggcag gcttgagcat attggaagca tccatagcgc
     8101 cttgcgcagc gccggctccg actacggtgt gcagttcgtc aataaacagg atgacttctc
     8161 cgtttgattt ttgaatctcg tcgatggcag cttttaaacg ctcctcaaac tcgccgcgga
     8221 agcgggtgcc ggcaatcatc gcgcccaagt cgagcgcgac cacgcgttta tcttccagga
     8281 tttccggcac gtcgttgttc gcaatttttt gcgccaggcc ttccacaatg gcggttttcc
     8341 caacgccagc ttcgcctatc agcacggggt tgttcttggt gcggcggcag aggatctgga
     8401 taagacggga aatttcctct tcgcgtccgt aaatcggatc cagcttgcct tcccgggctg
     8461 cctgggtaag gttgcgcgag aatttgtcca gcgttttgta ctgggtctcg ctgtgggttt
     8521 cggaacgctt actccctccg cggatttcca ggatggcgtc catcactttc tcgcgaatta
     8581 cgccgtggtt ctccagaatg cgcgccaggg cggtgtttcg ttccgccagg atggcaagaa
     8641 acaggtgctc cgtcgagata tattcatccc caaagcggct ggcttcattt ttggcaaggt
     8701 caaccgccaa tttcacgcgc ggggtcatga aaatctggcc cgaagctccg ccaaagatgt
     8761 tggccttggg ggtcgtccgc aaaaggtggt cgatgctatc ggctaattgc cccgcgtcca
     8821 gcttgagcat gtccagaatc tgagagacca tgccttccgg ctgctcaatc agcgccagca
     8881 tgatatgctc cgtatctatc tgcgtgtgtc cataacgctg gatgatttcc gcggcgcgcg
     8941 cggccacatc ctgcgctctt tcagaaaatt tatcaaaacg catcataggt atatgtcctt
     9001 aaatgttatt ttccgtacaa gttctgttgc aatacttgtt ccagcatata gtttaaccgc
     9061 caggcgtaag aattgaatta acgttagagg atgagcatcg cg
//
LOCUS       DAOQAD010000074         9010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_9157_length_9010_cov_5.540369, whole genome shotgun sequence.
ACCESSION   DAOQAD010000074 DAOQAD010000000
VERSION     DAOQAD010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9157_length_9010_cov_5.540369"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKL60_06555"
     CDS             complement(<1..288)
                     /locus_tag="PKL60_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95829.1"
                     /translation="MQNISVMTVQYWHHSFEFVLDSIEKCGLKSLELWAGDPHYSFDD
                     YTDRASASRKIARMRKQIEDRGMKVVMLTPEQLNYPINLAGRAEEYRQKSIR"
     gene            541..765
                     /locus_tag="PKL60_06560"
     CDS             541..765
                     /locus_tag="PKL60_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95830.1"
                     /translation="MTENKKHVPWYLWPFWLIYQLTLGILKFTGRLVGAILGLVVLIL
                     GALLCATLIGAILGVPLIILGLMMMVGSIF"
     gene            complement(786..1835)
                     /locus_tag="PKL60_06565"
     CDS             complement(786..1835)
                     /locus_tag="PKL60_06565"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HNW95831.1"
                     /translation="MEKIENPQDPENMLGYIDALAEDLEKAWQLGFSLPLPAFDHIER
                     VVIAGMGGSAIGGDLLSAYLAPSCRVPIFSHRNYGLPGWAQGQGTLVVCSSHSGNTEE
                     TLSSFDAALERGCQVMALCTGGSLRARAEENGKPVWVFEHHGQPRTAVPVSFGILLAL
                     LCRLCLAQVPESELQEAFTVLREGKAQFSMPSELSANPAKRLAGQLLERNVAVFAAGE
                     LEVVARRWKTQINELAKAWAVFEGLPESNHNTLAGLESPQSLLERTSALFLRSGLDHP
                     RNALRLTATQQAFLMAGASVDSVYARGAGRLAQMWSLLQFGDYVAYYLALAYGVDPTP
                     VDALTRLKHSLAAVQ"
     gene            complement(1876..2643)
                     /locus_tag="PKL60_06570"
     CDS             complement(1876..2643)
                     /locus_tag="PKL60_06570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW95832.1"
                     /translation="MTDYVDFNSQMWDQWSEAGNTWTIPVTHEDFVRAQNGNLNIYLT
                     PQKPVPQEWYRGLGKKVLGLASGGGQQGALLSAGGFEVTIFDNSRRQLAAEKRVADRE
                     GYRIGLVKGDMTRIFPFEDESFDWIINPVSNCYVEDLSNMWSEAFRVLRKGGALMTGF
                     TNPIVYMFADEDMDLNARTPLACKFKLPYNGRHLEAAGKIISMDEGYQFSHSLETQLG
                     GQTRAGFLIKAVYEDTDDSCRLSKYCSLYLADLAVKL"
     gene            complement(2662..3390)
                     /locus_tag="PKL60_06575"
     CDS             complement(2662..3390)
                     /locus_tag="PKL60_06575"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNW95833.1"
                     /translation="MVSLKKLRLLAACLLCAALALVPAACAPAGAEQSALPTAESLAQ
                     TLAAEATQLFQLTLVAFQNATPTARLTPAPSAEPTRIPTIARSPTPAQTSTASAGCNR
                     AAAGVPFDVTIPDESPMQPGQRFIKTWRLVNNGTCKWTRLYTLVFFSGNPLGALQSQL
                     LTAEVQPGAQVELSVEMFAPLEMGFYQSNWMLMAPDGELFGLGPNGDAPFWARIQVME
                     KFTDTPAPTSIATETPTPTPTPTP"
     gene            3810..7391
                     /locus_tag="PKL60_06580"
     CDS             3810..7391
                     /locus_tag="PKL60_06580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNW95834.1"
                     /translation="MTAETDAATSSKPADRWLDKPVVPWWPALTIEKLIIILLICAVF
                     LTRFYNLGARTMAHDEVNHVVPSYSIESYVYDPVTHGPLQFHLLALSFFLFGDSDFSA
                     RVPAALFGVAVVLFTIFAWRRYLGRVGALISAFLFVISPYILFYSRYTRNEIFIVFWG
                     LVLLWLFLRYLEGGESKYLLWITLITALHYADKATSYIFSAEALIFLAVLFLVESLRK
                     PWRERQYRSNFLIMTLVALLAVLAGAALYLLSKGAAAGSMLLPFAIIGAVLLAALVFM
                     IFWLIRGLGWKEIRLTRSFDLVVLQLLLILPLLTALPIRLLGFDPLDYSSAGILRSVA
                     VFLALSLVSMLLGLMWNRKVWLRALLIFWSIFIVFYTTFFTHGEGFFKGLVAALGYWM
                     SQQAVQRGTQPLYYYALVQIPVYEYLPALGMLLAVVIAALRGLFSSQPGSPFGRPMSA
                     ALAHSSESPASSSAPSSMTPEEQAELPADMGGEAQADAAGDGSLSIDTDLSLSPFTAE
                     DETRASQPAQADLPTVRQAWYERVFSLPPESHKTAAWVPTVALLIYWSLMSLLTFSYA
                     GERMPWLTTHISMPMILASGWALGYLVEHTDWEAVRRKKGWLVALLSLVFLVAFSSML
                     GALLGSVPPFSGKDLVQLQATSTFLLAAVGALASLAGLVFLLKDWQPRTFLKTLLLTC
                     FGVLAVLTFRTAYRAAYINYDNAKEYLVYAHSTRDMKDVLEQVETISKRLYGDKSIAV
                     AYDNDALYPFWWYLRDYPNRVWFGDKITKDLRNSPVILVGSANFAKIEPLVRDDYIMY
                     EYKRMWWPMEAYRNQSFGSLWQMLKNPAMRSALWEIWFNRDYTQYAAATGDGSLTLAT
                     WSPSDLLRMYIRKDVAAQIWEYGVEPVEAAPKVDPYAGSTISLDPVKVISSAGNLTFS
                     APRGLAVAPDGSLFAADSRNHRIVHLDANGLYLNSWGGYANVLEGPAPQGLFNEPWGV
                     AVGPDGLVYVTDTWNHRVQVFTPEGEFLRMWSEFLVDGVLDSFWGPRGIAVDARSRVY
                     VTDTGKQRVVVFDSQGNYLTQFGSRGMGIGQLDEPVGIAVDAAGKVYVADTWNNRVQV
                     FSPNADGTVFTSVLTWDVDAWTTDSLDNKPFLALSPDDQVILTDPDLGRVIRFDNQGV
                     FLQLWGGYQNSFLMGIISGVAVGMDGSVWVSDALNNTLLQFVPPAVP"
     gene            complement(7615..7968)
                     /gene="rplT"
                     /locus_tag="PKL60_06585"
     CDS             complement(7615..7968)
                     /gene="rplT"
                     /locus_tag="PKL60_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNW95835.1"
                     /translation="MARVKSGPYRHRRHQRRIAHNSGYRGTRNHLFKRANEAYMKSQW
                     YAYNDRRNRKRDLRRLWIVRINAAARQNGITYSRLISALRQSEIILNRKVLADLAVRD
                     PKAFSQVVSKATSKA"
     gene            complement(8058..8294)
                     /locus_tag="PKL60_06590"
     CDS             complement(8058..8294)
                     /locus_tag="PKL60_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNW95836.1"
                     /translation="MPRKQSSGKYKLKTHKATSKRFRTTGTGTVVRTKIGKGHLRRNT
                     SKRTKALLSETIAVKSSGLVKHIKRLAPYMSKKD"
     gene            complement(8396..8947)
                     /gene="infC"
                     /locus_tag="PKL60_06595"
     CDS             complement(8396..8947)
                     /gene="infC"
                     /locus_tag="PKL60_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNW95837.1"
                     /translation="MLFVKVYQGEEKISSKQEYRINERIRVEKVRLVDENGENVGIVS
                     TSEALRRADDAGLDLVEVAPNADPPVCRIMNFSKFLYDKEKKEREARKAQTKIEVKEI
                     RLRPKTGDHHRSFKVDDARGWLKNGMKVRVTIRFRGREITYPELALEDLREIAEDLKD
                     VSEIEQAPNMEGRTMFMLLSPKK"
BASE COUNT         1738 a         2599 c         2523 g         2150 t
ORIGIN      
        1 gcggatgctc ttctggcggt attcctcagc acgcccggcc aggttaatgg ggtaattcag
       61 ttgttcaggg gtcagcataa ccactttcat tccgcggtct tcaatctgtt tgcgcattct
      121 ggcgattttc cggctggcgc tcgcccggtc ggtataatca tcaaaactgt aatgcgggtc
      181 acctgcccaa agttcgaggg acttgagtcc gcatttttcg attgaatcca gcacgaattc
      241 aaaagaatgg tgccaatatt gcaccgtcat tacggaaatg ttctgcatgc taatccggga
      301 catcattctc cattcgtcaa gccgtaaata gataagttat agataatgtt ataccatatt
      361 aacagataag attcaagaaa ttttaagatt cacaaggcta atccccgctg tatcttctcg
      421 cttaggcttt gtaaaagtaa atcgcccaca atgcacagat tatcataggc gccagctcta
      481 caggtcagat atattcatag ccaagggaac cggagttcac cggttcaaat cggagagctg
      541 atgacagaaa acaaaaaaca cgtgccctgg tacctctggc ctttctggct gatttaccag
      601 cttacgctcg gaatcctcaa gttcaccgga cgacttgtgg gcgcaatcct tgggttggtg
      661 gtcctgattc tgggcgcgct gctgtgcgcc acgctcatag gcgccatcct gggcgtgccg
      721 ctgataattc tgggcctgat gatgatggtg ggctcaatct tctgagcacg ctccattcca
      781 tcagattact ggaccgctgc caggctgtgc ttcagcctgg tcagcgcgtc caccggcgtc
      841 gggtccacgc cgtaggcgag cgcgagataa taagcaacat aatccccgaa ctgaagcagg
      901 ctccacattt gcgccaggcg gcctgccccc cgggcataca cgctgtccac gctggcgcca
      961 gccatcagga aggcttgctg cgtagcggtc agtcttaacg cgttgcgcgg atggtccagc
     1021 ccggagcgta ggaacagcgc ggaagtcctt tccagcaagc tctgcgggga ttccagccca
     1081 gccagggtgt tgtgattgga ttcaggcagc ccctcgaaaa ccgcccaggc ttttgccagt
     1141 tcattgattt gggttttcca gcggcgcgca accacctcca attcccctgc cgcgaaaacg
     1201 gccacattcc gctccagcaa ttgtcccgcc aggcgtttgg cggggttggc gctcagctcg
     1261 gatggcatgc tgaactgcgc cttaccctcg cgcagcaccg tgaacgcttc ttgcagctca
     1321 ctctcaggca cctgtgccag gcaaagccgg caaagcagcg ccagcaagat gccaaaggag
     1381 accgggaccg ccgtgcgggg ctgtccatga tgctcaaaaa cccagacagg cttcccgttt
     1441 tcctccgcgc gcgcgcgcaa actgccgccg gtgcacagcg ccatcacctg gcagccgcgc
     1501 tccagcgccg cgtcaaagga ggagagcgtc tcttccgtgt tgccggaatg tgacgaacaa
     1561 acgaccagcg tgccctggcc ctgcgcccag cccggcaagc cgtaattgcg gtgggagaaa
     1621 attggcaccc ggcaggacgg cgccagatac gcgctgagca ggtcgccgcc aatcgcggaa
     1681 ccccccatgc ccgcgatgac gacccgctcg atatggtcga acgccggcag cggcaggctg
     1741 aatccgagct gccaggcttt ttccaggtct tccgccagcg cgtcgatgta tccaagcatg
     1801 ttttcggggt cctgcgggtt ttctattttc tccattaggg atgctcctgt tttttgactt
     1861 aatctcaaac gaaaatcaaa gtttgactgc caggtccgcg agataaaggc tgcagtattt
     1921 gctcagccgg cagctgtcgt ccgtgtcctc gtacactgct tttatcaaaa agcccgcccg
     1981 ggtttggccg ccaagctggg tttccaggct gtggctgaat tggtagccct catccatgct
     2041 gatgattttc ccggcggctt ccaggtgtct gccgttatag ggcagtttga atttgcatgc
     2101 caacggggtg cgggcgttca agtccatgtc ctcatccgcg aacatataaa cgattggatt
     2161 tgtaaagccg gtcatcagcg ccccgccttt gcgcaacacg cgaaaagcct cgctccacat
     2221 attgctcagg tcctcgacgt aacaattgga aaccgggttg ataatccaat cgaaactttc
     2281 atcctcgaaa ggaaatattc tggtcatatc acccttcacc agcccaatcc ggtagccttc
     2341 gcggtctgcc acacgctttt cagctgccag ctgtcggcgg gaattatcga aaattgtgac
     2401 ctcaaacccg cctgctgaaa gcagcgcccc ttgctggcct ccgccgctgg ccagccccag
     2461 cactttcttg cccagtccgc ggtaccattc ctgggggact ggcttttgcg gggtcaggta
     2521 aatgttgagg ttcccgtttt gggctcgcac aaagtcttca tgggtaaccg gaatcgtcca
     2581 ggtgttgcct gcttcactcc attggtccca catttgtgaa ttgaaatcca cgtaatcggt
     2641 cattgctgct ctcctgtaaa ctcagggggt tggggttgga gttggtgttg gggtctcagt
     2701 cgcgatcgag gttggcgcgg gcgtgtcggt aaatttttcc atcacctgaa tgcgcgccca
     2761 aaagggggca tccccgttgg gacccagacc aaagagctcg ccgtccggag ccatcagcat
     2821 ccagttgctt tggtaaaatc ccatttccaa cggcgcgaac atctctacgc tcagttccac
     2881 ctgcgcgcca ggctgcactt cggcagtcag cagctgcgat tgcagcgctc ccagcgggtt
     2941 gccggaaaag aaaaccagcg tatacaggcg ggtccacttg caggtgccgt tgttcaccag
     3001 ccgccaggtc ttgataaagc gctgacccgg ctgcatgggg ctttcgtcgg ggattgttac
     3061 gtcaaacggc acgccggctg ccgcccggtt gcagccagcg cttgcagtgg acgtttgggc
     3121 gggcgtcggg ctgcgcgcga ttgtcggaat ccgggtcggc tcggcactgg gcgcaggagt
     3181 caggcgggcg gttggcgtgg cgttctgaaa agccaccagg gtaagctgaa acagctgcgt
     3241 cgcttcggca gccagcgttt gcgccaggga ttctgccgtg ggtagagcgc tttgctcagc
     3301 ccccgcgggc gcgcacgccg ctggaaccaa ggcaagcgcc gcacataaca gacaggcggc
     3361 cagtaatctt aattttttta aagagaccat ctcaggttaa ttttactcta cttggcagtt
     3421 aagggcttct gcgccgcaag cgcgcggccg tcctccttcc ttccgggtgg attctgacag
     3481 cacggctggg taacgagccc ggctcaggcc gaagcgttca atagagcaac agaaaatcgg
     3541 gacaggattt gccttatcct ttgctgttcg cggttctccg tctatcagga aacttgagca
     3601 gttccaaagc aacggcttta tcggctgcct caacagccgg caagggaaaa tggaattgta
     3661 cccggccacc agccgtcctt tacagcaaac cccgctgtgc aggcgaaaca ttcaccagtt
     3721 ttgctctgtt ctccgcgctt gccttgcctc cacaaatgga gtaaagtata gtttcacgga
     3781 gaaatgttcc tcagaacgga gacccattta tgactgctga gacggatgcc gcgacctcgt
     3841 ccaaacccgc cgaccgctgg ctggacaaac ctgttgtgcc ctggtggccg gcgctgacca
     3901 tcgaaaaatt aataataatc ctattgattt gcgcggtttt cctcacgcgt ttctacaatc
     3961 ttggcgcgcg caccatggca catgatgaag ttaatcatgt ggttccgtcc tattccatcg
     4021 aatcgtatgt ctacgacccg gttacccatg gtccgcttca attccacttg ctggcattgt
     4081 ctttcttcct gttcggagat tccgatttca gcgcgcgggt acccgcggcg ctgtttggcg
     4141 tggcggtggt tcttttcacg atttttgcct ggcggcgcta cctcggtcgg gttggcgcgc
     4201 tcatcagcgc ctttctattc gtcatctcgc cgtatatcct gttctacagc cgctataccc
     4261 gcaacgagat atttattgtc ttttggggtt tggtgctgct ttggctgttc ctgcgctatc
     4321 tggaaggcgg cgagagcaag tacctgctct ggattacgct catcaccgcg ctgcactacg
     4381 cagataaagc cacctcttac atcttcagcg cggaagcgct cattttcctg gcggttctct
     4441 ttctagtcga atccctgcgt aagccctggc gggagcgcca ataccgctcg aatttcctca
     4501 tcatgaccct ggtcgccttg ctggcggtgc tggcaggtgc ggcgctgtat ctgctgagca
     4561 agggcgcggc agctggcagc atgctgctcc ccttcgcgat tatcggcgcg gtgctgctgg
     4621 cagctcttgt ttttatgatt ttctggctga ttcgcggcct gggttggaaa gaaatccgcc
     4681 ttacccgcag ctttgacctg gtggttttgc agctgctgct tatcctgccg ctgctgaccg
     4741 ccctgcccat caggctgctc ggttttgacc cgcttgacta ctcgtccgcc ggcatcctgc
     4801 gctccgtggc ggttttcctg gcgctcagcc tggtgtccat gctgctcggg ctaatgtgga
     4861 accgcaaggt ctggctgcgc gcgctgctga ttttctggag cattttcatt gttttctaca
     4921 ccaccttctt cacccacggc gaaggcttct ttaagggtct ggtagccgcg ctgggttatt
     4981 ggatgtccca acaggcggtg cagcgcggca cacagccgct gtattactac gccctggtgc
     5041 aaattccggt atacgaatac ctgcccgcgc tgggtatgct gctcgcggtg gtcatcgccg
     5101 cgctgcgggg gctgttcagc tcccagcccg gcagtccgtt cggccgcccc atgtctgctg
     5161 cccttgcaca ctcgtctgag agcccggcga gctccagcgc tccatcatcg atgacgccag
     5221 aggaacaggc ggagctacct gcagacatgg gaggcgaagc ccaggctgat gctgcgggag
     5281 atggctcact ttccatcgac acggatttat cgctaagtcc gtttacagca gaggatgaga
     5341 cgcgcgcgag ccaacctgcc caagccgacc tgcccaccgt gcggcaggcc tggtacgagc
     5401 gcgtgttcag tctgccgcct gagtcacaca aaacggctgc ctgggtcccg accgtcgcgc
     5461 tgctaatcta ctggtccttg atgagcttgc tgaccttctc ctacgcaggc gaacgcatgc
     5521 cctggttgac cacgcacatc agcatgccga tgattcttgc cagcggctgg gctttgggat
     5581 acctggtaga gcacaccgat tgggaagctg tccggcgcaa aaaaggctgg ctggtcgcgc
     5641 tgctcagcct ggtgttcctg gtcgctttca gcagcatgct gggcgcgctg ctcgggtctg
     5701 taccgccttt ttcggggaaa gacctggtgc agctgcaagc caccagcaca ttcctgttgg
     5761 ccgcagtggg cgctcttgcc agcctggcgg gcctggtttt cctgttgaaa gactggcagc
     5821 cgcgcacctt tttgaaaaca ctgctgctca cctgctttgg agtcttggct gtgctcacct
     5881 tccgtaccgc gtaccgtgcc gcttacatca attatgacaa cgccaaggaa tatctggtct
     5941 atgcgcactc cacgcgcgat atgaaggatg tgctggaaca ggtggagacg atttccaaac
     6001 gcctgtacgg ggataagtcc atcgcggtcg cctacgataa cgacgcgctc tatcccttct
     6061 ggtggtacct gcgcgactac cccaacaggg tctggtttgg cgataaaatt accaaggact
     6121 tgcgcaactc gcctgtaatc ctggtgggca gcgccaattt tgccaagatt gagccgctgg
     6181 tgcgggacga ctacatcatg tacgaataca agcgcatgtg gtggccgatg gaagcctacc
     6241 gcaaccagag ctttggctcg ctgtggcaaa tgctgaaaaa tccggccatg cgttcggcgc
     6301 tgtgggaaat ttggttcaac cgcgattaca cacagtatgc cgcggcgacc ggcgacggaa
     6361 gcctgaccct ggcgacctgg tcgccttcgg acttactgcg catgtacatc cgcaaggatg
     6421 tggctgcgca aatctgggaa tacggggttg aaccggtgga agccgccccg aaagtggacc
     6481 cgtacgcggg cagcactatc agcctggacc ctgtaaaggt tatcagttct gccggcaacc
     6541 tgactttctc cgcgccgcgc gggttggctg tcgcgccaga cggcagcctg ttcgcagcgg
     6601 attcgcgcaa tcaccgcatc gtgcatctgg acgcgaacgg cctgtacctc aactcctggg
     6661 gcggatacgc caatgtgctg gaaggacccg ccccgcaggg cttgttcaat gagccctggg
     6721 gcgtggcggt tggaccggac ggattggtat atgtaaccga cacatggaac caccgcgtgc
     6781 aggttttcac tcctgagggc gagttcctgc gcatgtggag cgagtttctg gtggacggcg
     6841 tgctggactc cttttggggt ccgcgcggca ttgccgtgga cgcccggagc cgggtctatg
     6901 tgaccgatac cggcaagcag cgggtggttg tttttgacag ccagggcaat tatctcaccc
     6961 agtttggcag ccgcggcatg ggcatcgggc agttggacga gccggttggc atcgccgtgg
     7021 atgccgctgg caaagtgtac gtggcagata cctggaataa ccgcgtgcag gtcttctccc
     7081 cgaacgcaga cggcactgtc ttcaccagcg tgctcacctg ggatgtggac gcctggacga
     7141 ctgactcgct ggacaacaag cccttcctgg ctctgagccc ggatgaccag gtaattctca
     7201 ctgacccgga ccttgggcgg gtaatccgtt ttgacaacca gggcgttttc ctgcaattgt
     7261 ggggcggcta ccagaactcc ttcctgatgg ggatcatcag cggcgttgct gtcgggatgg
     7321 acggcagtgt gtgggtctcc gatgccttga acaataccct gctccagttt gtgccgcccg
     7381 cggttccgta agtccgcaag cattcttgaa gaaaaagagg cagttcggag ataaactgcc
     7441 tcttttcgtt tttgccagaa ccagctctgc cgcggatact tttccgtccg gaatacaatt
     7501 cgtttgctgt tggagctaag atgcccttgg agaagagcgg aatagaaggg gaacctggtg
     7561 aaacaaacaa cccctgagcg tgtcaggggc tgtatgttct cggtttgaaa aaactcacgc
     7621 cttagaggta gccttgctta cgacttgcga gaaagctttg gggtcccgta cggccaggtc
     7681 agccagaacc ttgcggttca gaatgatttc tgactgccgg agcgcgctga tcagccggct
     7741 gtaggtgatg ccgttctggc gggctgccgc gttgatccgc acaatccaga gcctgcgcaa
     7801 atcgcgtttg cgattgcgtc ggtcgttgta agcgtaccat tggcttttca tgtacgcttc
     7861 attggcgcgc ttgaacagat gatttcgggt tccccgataa ccgctattgt gggctatccg
     7921 gcgctggtgg cgtcggtggc ggtatggtcc actctttact cttgccattt tgtaatctcc
     7981 tttgcggtcc cctgggcgtc agatttgcat ccagttgcat acacccagca gccgcgctaa
     8041 aacgaatcga caggacctta gtcctttttg ctcatgtagg gcgccaaacg cttgatgtgc
     8101 ttgacgagac cgctggattt tacagcgata gtctcggaaa gcagagcttt cgtacgcttg
     8161 gaggtattgc ggcgcagatg acctttcccg attttggtgc gcacaacagt accggttcca
     8221 gtggtacgga agcgttttga cgtggcctta tgagtcttca acttgtactt tccactgctt
     8281 tgttttctgg gcacgttcaa actcctctaa gaaaatgcaa cggctgtgaa ccgaccgcag
     8341 ccgttggtat taataccgac ctatcattat aacagattaa cgcagagtca agaaactact
     8401 ttttcgggct gagcagcatg aacattgtgc gcccttccat attcggcgcc tgttcaatct
     8461 cgctcacatc cttcaggtcc tcggcgattt cgcgcaagtc ttccagcgcc aattcggggt
     8521 aggtaatctc ccgcccgcgg aagcggatgg tgacgcggac tttcatgccg tttttcagcc
     8581 aaccgcgcgc gtcatccacc ttaaagctgc ggtgatgatc gccggttttc gggcgcagcc
     8641 ggatttcctt gacctcgatt ttggtctggg ctttacgggc ttcacgttcc ttcttttctt
     8701 tatcgtagag gaacttgctg aaattcatga tccggcacac tggcggatcg gcattgggag
     8761 cgacttccac caggtccaga ccggcgtcgt ctgccctgcg gagtgcttcg ctggtggaga
     8821 caatgccgac gttttcgccg ttttcatcca ccaggcggac tttctcaacc ctgatgcgtt
     8881 cattgattct gtattcttgt ttactactaa tcttctcttc tccttggtat accttaacga
     8941 ataacatatc atctgatggt atgttcaacg ggtaatatta ccatatacgc gcggtaatcg
     9001 tcaactggag
//
LOCUS       DAOQAD010000075         8772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_9502_length_8772_cov_5.697602, whole genome shotgun sequence.
ACCESSION   DAOQAD010000075 DAOQAD010000000
VERSION     DAOQAD010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9502_length_8772_cov_5.697602"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PKL60_06600"
     CDS             <1..498
                     /locus_tag="PKL60_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNW95838.1"
                     /translation="QTSERLLTLMEDDDAEEVRTLLGYPETSSGGIMTTEFAWVPYTY
                     NVEQALTHLRSSEEALEDEFMYYVYLLDEQERLKGVVSLRDLVTAPLDQPLSKWIDDD
                     IAQVTPLTPQEEAAYLVAKYDLMALPVVEPESKKMIGIVTVDDALDTVLPTAWKKKLP
                     RFSGK"
     gene            511..1764
                     /locus_tag="PKL60_06605"
     CDS             511..1764
                     /locus_tag="PKL60_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNW95839.1"
                     /translation="MKRQFKFPFWLRRIFLFFTIIGPGLITASADNDAPGIATYSISG
                     AKYGYGFLWIIVLITVGEVLVQEMAARMGAVTGKGTADLIRERFGVRTSAFAMACLLL
                     ANIGTTLAQFAGVAAVGELFGISRFLTVPLAGLLIGLIILRGNYKNIETVLLILCLSA
                     FSYVITVFVIKPDWGIILKEALTPTIKLESDYLLAVLAVIGTTITPWGIFYLQASIAD
                     KGTDLKDYNHTRLDVVFGSVWGNIISAFIIITTAATLYPKGILVNSAEEAAMALSPLA
                     GSFSSILFSVGMLGASLLAVSVLPLSTTYAMCEAFGFERGLNRPVKEAPVFYSIFVGI
                     MVISMLFVLIPGIPLFRIMLLSQSVNAILLPVILLLVLSLANDRNIMGEWTNKRFANV
                     LGYALTGLIFLATIALLVGMFLPAA"
     gene            complement(1767..2777)
                     /locus_tag="PKL60_06610"
     CDS             complement(1767..2777)
                     /locus_tag="PKL60_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95840.1"
                     /translation="MTRLVLSACDLQIGFQPSRVSRKVVASGLNLDLHGGEFVCLAGP
                     NGVGKSTLLRTLTRLQPALGGEVRLSGRPLREYSSKELAGLVSVVLTGQVEVNAMRVR
                     ELVGLGRFPHTGIFDSRSQEDERAVENALALTDVSQLADRFVQELSDGERQRVMIARA
                     LAQEPLLLVLDEPTAFLDLPGRISIMHLLRDLARGRGQAVLTSTHDLDLAMRSADRIW
                     LMNRGGSIAQGSPEDLVLSGQFGHTFSQANLRYNMSTGQFEEEHAGARPVVLRAEGLA
                     GAWTAHALRRAGFEVLVEGAEYLPLIEVEGQGGANCWRLTWQGVSRELSSIYALLSAL
                     LE"
     gene            complement(2774..3853)
                     /locus_tag="PKL60_06615"
     CDS             complement(2774..3853)
                     /locus_tag="PKL60_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNW95841.1"
                     /translation="MRPRSDLTPAASKPLHKGWIIALFVLILLAAFVLSLSLGSVSIP
                     MRQVFGILSGKPAFKESWKTIVLDFRLTKALTAVLTGAALSVAGLQMQTLFRNPLADP
                     FILGINSGASLGVALAVLAIGTTGSILLAGISTAGDLGLALAASIGAGLVLGLILLVG
                     RRVKSPITLLILGLMFGYAASALTSLLIYFSLPERIQAYTVWSYGSFSGVTWSQLRVM
                     APVILAGLVLTVFLPKPLNALLLGEEYARSMGVNTRLTRVLILLSASILAGTVTAFCG
                     PIGFIGVAVPHLCRNLLRSADHKLLLPAVGILGAALALLADAIAQLPGSQYVLPINVV
                     TSLFGAPFVVWVIIQQRKGRTSFSV"
     gene            complement(3861..5027)
                     /locus_tag="PKL60_06620"
     CDS             complement(3861..5027)
                     /locus_tag="PKL60_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW95842.1"
                     /translation="MKKTTRILLLLLVLVLTACSAISAGEAPAKESGQTELRVFPIRF
                     AQHFTLEYADGYKKLTVTQPWAGATEALTYLLVPRGQPAPADAGNALVIETPVRTFVS
                     MSTTYYPFLEKIGKLDALVGVDDATYAYNPQVRARAADGSLALVGGGAGGGSVNVEKL
                     LELNPDLIMTSASGSPDLDAHPKLLEAGLPVVINADYLEQSPLGRAEWGIFIAAFFDL
                     EEAASQDFDALVQRYEAAKKLTQGLEDKVSVFTNTDYQGTWYVPGGESYAALLLKDAG
                     ADYIFADQPGYGAAPLAFEAVFDAAKDADYWLNVGFAADLDSLLAMDARYAEFKAFAQ
                     GSVYNYNARVTETGGSDYFESGVANPDVILKDLIRIFYPDLLPGYELYYYQQLS"
     regulatory      complement(5120..5256)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5686..6336
                     /locus_tag="PKL60_06625"
     CDS             5686..6336
                     /locus_tag="PKL60_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95843.1"
                     /translation="MKKSSLKSLPEIELARFQSLQKGNDCTLHAISAALELLCGVRLA
                     PRELAAEVNRLWWRGRFYRVLPGWAVTPPMQARIVNYLARTRCLPVRARLLHTSPEIL
                     RNLPGDDNLAALATIYWRRGRVPAIYRGATAWNYNQSKSTAGHTMLFAAYDPEHTNPG
                     GLQTPWGFINSWADANTELFWMEDEPFRRAWGVGLPIIGRNATVLISLDPQPADTK"
     gene            6333..8072
                     /locus_tag="PKL60_06630"
     CDS             6333..8072
                     /locus_tag="PKL60_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95844.1"
                     /translation="MKAYSLLFSLAKRYARSIILTILTMLGLVAAQLIIPWLIRTLIS
                     TLSASSLPGNALETITRLSAIALLVFVARGFMQFVRSYQAHIAGWGVVADSRKLVYQQ
                     LQKLSLRYYEDKQTGQLMSRVINDTELFERMIAHALPDVFVNAVTLIGVTAILFSVSW
                     QLTLLTLIPIPLIALTLIVYAKLVRPAFRYRQSELGELNAALNDSITGVREIKAFAHE
                     DLAMVEVGTRIENYLRSNLKALKIMAIFTPILDFAAGIGQLIVIFFGSRLALAGSLQI
                     ADLVAFFLYLDSFYQPVRNLNNAWEAVQESMAGFERVAEILNETPDITEPEQPVPLPQ
                     PVRGALRFENVNFHYTPQETILKDITLDIPAGSTLALVGPTGVGKSTLASLIPRFYDV
                     SGGRITLDGVDIRCLDTKELRSQISMVLQDVVLFHGSVRENILFGNPQASEEEMLTAA
                     KIANVQEFVSAMPDGYDTMIGERGVKLSGGQKQRISIARALLKNSPILILDEATSSVD
                     TETEMLIQEALEHLIQGRTTIIIAHRLTTVRNADQIAVLEGSTITEIGTHADLLAREG
                     LYHRLYSIQQRLN"
     gene            complement(8123..>8772)
                     /locus_tag="PKL60_06635"
     CDS             complement(8123..>8772)
                     /locus_tag="PKL60_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNW95845.1"
                     /translation="PMPTSPYLIPAAPAESEILVVNSRFIASLSPAFSVEEARAFHKS
                     IRQRFPDATHHVPAFVIGHGNSVITHCSDDGEPSGTAGRPALAVLQGSGLGDAAVVVT
                     RYFGGTLLGTGGLVKAYGDAVREVLKLVRRAIKVPTTTIGVQLPYPLFEQVQRLAAAH
                     GGEQLESQFLADVTLILRFRDEELDAFLQQLTNLSAGQIQPVVLERNPETIFPLP"
BASE COUNT         1829 a         2703 c         2477 g         1763 t
ORIGIN      
        1 cagaccagtg agcgcctgct gaccctgatg gaagacgatg atgccgaaga agtccgcacc
       61 ctgttgggct acccagaaac ctcatcgggc ggcatcatga ctacagagtt tgcctgggtg
      121 ccctacacct acaatgtgga acaggcgctg acgcatctgc gctcttccga agaagccctg
      181 gaagatgagt tcatgtacta cgtctacctg ctggatgagc aggaaaggct gaagggtgtc
      241 gtcagcctgc gggatttggt gaccgctccg ctggaccagc cgctttccaa atggattgac
      301 gatgacatcg cgcaggtaac ccccctcacc ccgcaggaag aagccgccta tctggttgcg
      361 aagtacgacc tgatggcgct gcctgtggtg gagccggaaa gcaaaaagat gatcggcatt
      421 gtcaccgtgg atgacgcgct ggatacggta ttaccgaccg cgtggaaaaa gaaactgcca
      481 agattttctg gaaaataggc cgggggaatc atgaagcgac aattcaagtt tcctttctgg
      541 ctgcgcagga tcttcctgtt tttcaccatc atcggcccgg gtctgattac cgccagcgcg
      601 gataacgacg cgccgggcat cgccacctat tccatctccg gcgcaaaata cggctacggc
      661 tttttgtgga tcattgtcct gatcacagtc ggcgaggtgt tggtgcagga aatggcggcc
      721 cgcatgggcg ccgtgaccgg caaaggcacg gcagacctca tccgcgagcg ttttggcgtg
      781 cgcacttctg ccttcgcgat ggcatgcttg ctgctggcga acatcggcac caccctggcg
      841 cagttcgccg gcgtagccgc ggtgggcgag ctttttggca tcagccgctt ccttaccgtt
      901 ccgctggccg gcctcctgat cgggctcatc attctgcgcg ggaactacaa gaacatcgag
      961 acagttttgc tcattctttg cctttccgcc ttcagttacg ttatcacggt cttcgtcatc
     1021 aaacccgatt ggggcatcat cctcaaagaa gccctgaccc ccacgattaa gcttgaatcg
     1081 gactacctgc tggcggtgct ggctgtcatt ggcaccacca tcaccccctg gggcatcttt
     1141 tacctgcagg cctccatcgc ggataagggc acggacctga aagattacaa ccacacccgc
     1201 ttggacgtcg tgtttggttc ggtttggggc aatatcatct ctgcttttat cataattaca
     1261 acagcggcaa cgctgtaccc aaagggcatc ctggtaaaca gcgcggaaga agccgccatg
     1321 gcgctcagcc cgctggccgg ttccttttcc agcatcctct tttcggtcgg gatgttgggc
     1381 gcttcgctgc tggcggtctc ggtgctgccg ctttccacca cttacgccat gtgtgaagcc
     1441 tttggcttcg agcgcgggct caaccgtccg gtgaaggaag ctccggtgtt ctattccatc
     1501 ttcgtgggga tcatggtaat cagcatgctt ttcgtcctga ttcctggtat tcccttgttc
     1561 cgaatcatgc tcctatccca gtctgttaat gccatcctgc tgccggtgat tctgctgctg
     1621 gtgctttccc tggcgaatga ccggaacatc atgggcgaat ggacgaacaa acgcttcgca
     1681 aacgtattgg ggtacgcgct aaccgggcta atcttcttgg ccacaattgc gctgctggtg
     1741 ggtatgtttt taccagctgc ctgagcttat tccagcagag cgctgagcag cgcgtaaatg
     1801 gaactgagct ccctggatac tccctgccag gtaagccgcc agcaattcgc tcccccctga
     1861 ccttccacct cgattaaggg caggtactct gctccttcta ccaacacctc aaagcccgcc
     1921 ctgcggagtg cgtgcgcggt ccacgcgcca gccagcccct cggcgcgcag cacgacgggt
     1981 cttgctcccg cgtgttcttc ctcaaactgc cccgtggaca tgttgtaacg cagattggcc
     2041 tggctgaaag tgtgcccaaa ttgtccgcta agcaccaggt cttccggcga gccctgcgcg
     2101 atacttccgc cgcggttcat cagccagatg cggtccgcgc tgcgcatggc cagatccaaa
     2161 tcgtgcgtgg aggtgagcac agcctgcccc cgtccgcgcg ccaggtcccg cagcaaatgc
     2221 atgatggaaa tccggccggg taaatccagg aaagcggtag gctcatcgag cacaagcagc
     2281 agcggttcct gcgccagcgc ccgggcaatc atcacgcgct gcctttcgcc gtcgctgagt
     2341 tcctgcacaa aacggtcggc aagctggctt acgtccgtca gcgccagcgc gttctcgaca
     2401 gcccgctcgt cctcctgcga acggctgtca aaaatcccgg tgtgcggaaa gcgccccaag
     2461 cccaccagct cccgcacacg catggcgttc acctcgacct gcccggtcag gaccacgctg
     2521 accaggccgg cgagctcttt gctgctgtac tcacgcagcg gccggccgct caggcgcact
     2581 tcgccgccca aagccggctg cagacgggtc agcgtccgca gcaaggtaga tttcccgacc
     2641 ccgtttggcc ctgccaggca cacaaactcg cccccgtgca ggtcaaggtt taagccgctg
     2701 gcaaccacct tgcggctgac gcgagagggc tggaaaccta tctgcaaatc gcatgcactg
     2761 aggacgagtc tggtcatacg gaaaacgagg tccttccctt gcgctgctgg ataatgaccc
     2821 acaccacaaa tggcgcgcca aacagagagg tcacgacatt aatcggaagc acatactgac
     2881 tgccgggcag ttgggcgatc gcgtccgcga gtagcgccag cgccgcgccg agtatcccga
     2941 ccgcgggcag cagcagctta tggtccgcgc tgcgcagcaa attccggcac aaatgcggca
     3001 ccgccacgcc gatgaacccg attggaccgc agaaggcggt gaccgtgcct gccaggatgg
     3061 acgcgctgag caggatcaag actctggtga ggcgcgtgtt cacccccatg ctgcgcgcgt
     3121 actcctcgcc cagaagcagc gcgttcagag gtttgggtag aaagactgtc agcacgagcc
     3181 cggcaaggat caccggcgcc atcacccgca gttgactcca agtgaccccg ctgaacgaac
     3241 cgtaggacca gacggtatag gcctgaatcc tttccggcag gctgaaataa atcaggaggc
     3301 tggtcagcgc gctggcggca taaccgaaca tcaagcccaa aatcagaagg gtgatggggc
     3361 tcttgacgcg ccgtccgacc aacaaaatca agcccaacac cagcccggcg ccaatggaag
     3421 ctgccagcgc cagccccagg tccccagccg tgctgatgcc cgccagcaaa atgctgccgg
     3481 ttgtgccgat tgccagcacc gccagggcta cccccaggct cgcgccggag ttgatgccga
     3541 ggataaaagg gtccgccagc gggttgcgaa acagggtttg catttgcaga ccagcgaccg
     3601 agagtgcggc gccagtcagc accgccgtaa gcgcttttgt caggcggaag tccagcacaa
     3661 tggtcttcca gctctctttg aaggccggtt ttcctgatag gatgccaaag acctgccgca
     3721 ttgggatgga cactgaacca aggctcaggc tcagcacaaa cgctgcaagc agaatcagca
     3781 cgaacagcgc gatgatccag cccttatgca gaggcttgct ggcagcgggg gtcaggtccg
     3841 atcgagggcg catttcagtt tcagctcaac tgctggtaat agtaaagttc atacccgggc
     3901 agcaggtccg gatagaaaat gcggatgaga tccttcagga tgacatccgg gtttgctaca
     3961 ccgctctcaa aataatccga gccgcccgtt tccgtcacgc gcgcgttgta gttatatacg
     4021 ctgccctgcg cgaaggcttt gaattcggca tagcgggcat ccatcgcgag cagactgtcc
     4081 aggtcagcgg caaagccgac gttcaaccag tagtcggcgt ctttggcggc gtcaaaaacc
     4141 gcctcaaatg ccagcggcgc ggcgccgtaa ccgggctggt ccgcgaaaat atagtcagcg
     4201 ccggcgtcct tcagcagcag cgccgcgtaa ctttcgccgc cggggacgta ccaggtgccc
     4261 tgataatcgg tattggtaaa gacgctgacc ttatcttcca ggccctgggt aagctttttg
     4321 gccgcttcgt agcgctgcac cagcgcgtca aagtcctgtg aggcggcttc ctcaaggtca
     4381 aagaaggcgg cgataaaaat gccccattcc gctctgccca acgggctttg ctccaggtaa
     4441 tcggcattga tgaccaccgg caggccggcc tccagcagct tcgggtgcgc gtccaaatcc
     4501 ggcgaaccgc tggcgctggt cataatcagg tccgggttca gttccagcag cttttccaca
     4561 ttcacgctgc ctccacccgc gccgcccccg accaaagcga ggctgccgtc cgcggccctc
     4621 gcccgcactt gcgggttgta ggcataagtc gcatcgtcca cccccacaag cgcgtccagc
     4681 ttcccgattt tctccaggaa gggatagtac gtggtggaca tggacacaaa agtgcgcacc
     4741 ggcgtttcga taaccaacgc gtttcccgcg tctgcgggcg cgggctggcc gcgcggaacc
     4801 agcaggtagg tcaaggcctc agtggcgcct gcccagggtt gggtaacggt cagctttttg
     4861 tagccgtcgg catactccag cgtgaaatgc tgcgcgaaac ggatggggaa aacgcgcaat
     4921 tcggtttggc cgctctcttt agcaggcgct tctccggctg atatggcgga acaagccgtg
     4981 agaacaagaa cgagcagcaa aagcaggatt cgggttgttt ttttcattgc cagctccaaa
     5041 taaaacaccc aactcgaatt aaggttgggt tagggttggt gtggaaaaaa catacccgcc
     5101 ttcttaatcc gagaaggtca taaagggatt tgattgaggc aggcttcctg gcttgtcttg
     5161 cgacttacag ttgcggtaca gcgctggact tacaccagct tacaccttta caccctggct
     5221 tccgggcatc gggttacctc gaattggctt gtgaatgtgc ctttgaaaca taccatgaag
     5281 gcgcggaact gtcaaaatcc gggtatatgc tggcggcggc ggatgcggaa accagccctg
     5341 gcagtccagg atcgctcgcc ttctctgcct cgtccgcata atcaggtgag cttcctgctg
     5401 gttattccgc gattggttct gcgagctcag ggccgccaat ctccagattt cgcccgctgc
     5461 ccaaaacaaa ccatactgag gtcctgccga aaaaacaccc aggcaggttc gctgctgagc
     5521 gcctaaaagc tcacgggcgt ggagcgaggc cctaaggatt atcttttgga ccttaaggca
     5581 gcgctgctgc tctcttggta atccatttgc agtccgcatg aaagaggtac tccgaaaaaa
     5641 tgtaaatcca gcccccaccc gcgcattcca atgtaaaatt ggcatgtgaa aaaaagctct
     5701 ctaaaatccc tgcccgaaat tgaactggcc cgctttcaat ccctgcaaaa aggcaacgac
     5761 tgcaccctgc acgccatttc cgccgcgttg gagctgttgt gcggtgtgcg cctggctccg
     5821 cgggaattag ccgcggaagt taaccgcctg tggtggcgcg gcaggtttta ccgggtgctg
     5881 cccggttggg cggtcacgcc gcccatgcaa gccagaatcg tgaattatct cgcgcgaacg
     5941 cgctgcttgc cggtgcgcgc gcgcttgctg cacacttccc cggaaatctt gcgaaacctg
     6001 cccggggacg ataacctggc cgcgctcgcg accatttact ggcgccgcgg acgcgtgccg
     6061 gcgatctacc gcggggcgac cgcctggaac tacaaccaga gcaagtcgac cgccgggcac
     6121 acgatgttat ttgccgctta cgacccggag cacaccaacc caggcggcct gcagaccccc
     6181 tggggcttta tcaattcctg ggcagatgcc aacacagagc ttttctggat ggaagatgaa
     6241 cccttccggc gtgcctgggg cgtcgggctg cccattatcg gccgcaacgc taccgtatta
     6301 atctccctcg atccacaacc agccgacacg aaatgaaagc atattccctc ctctttagcc
     6361 tggccaaacg gtacgcgcgc tctattatcc tgacaatcct caccatgctc gggctcgtcg
     6421 cggcgcagct catcatcccc tggctaattc gtacgctcat cagcacattg agcgcctcca
     6481 gcctgcccgg aaacgccctg gaaaccatca cccgcctgag cgccattgct ttgctggttt
     6541 ttgtcgcgcg cggtttcatg cagtttgtgc gctcctatca ggcgcacatc gccggttggg
     6601 gcgtggtggc agactcgcgc aagctggtct accagcagct gcaaaagctt tccctgcgct
     6661 actacgaaga caaacaaacc ggccagctca tgtcgcgcgt gattaacgac accgagctgt
     6721 ttgaacgcat gatcgcgcat gctttgccgg atgtgttcgt gaacgccgta acgctgattg
     6781 gggtcactgc catccttttc agcgtcagct ggcagctgac cttgttgacg ctcatcccca
     6841 ttcccctcat cgcgctgacc ctgatcgtct acgcgaaact ggtccgcccc gctttccgct
     6901 accgccaaag tgaactgggg gaattaaacg cggcgctgaa cgacagcatt acaggcgtcc
     6961 gggaaattaa agctttcgcg catgaagacc tcgccatggt cgaagtaggc acgcgcatcg
     7021 agaattacct gcgctccaat ctcaaagcgc tgaaaatcat ggcgatcttc acgcccatcc
     7081 tggactttgc cgccggcatc gggcagctga tcgtcatctt ctttggcagc cgcctggcgc
     7141 tggcgggctc cctgcagatt gccgacctgg ttgccttctt cctgtatctg gacagcttct
     7201 accagccggt gcgcaacctg aacaacgcct gggaagccgt gcaggaatcg atggctggct
     7261 ttgaacgcgt ggcggagatt ttgaatgaaa ccccggatat taccgagcct gaacagccag
     7321 tccccctgcc ccaacccgtg cgcggcgcgc tgcgctttga gaacgtgaac ttccattaca
     7381 ccccccagga aaccattctg aaagacatca cgttggatat cccggccggc agcactctcg
     7441 ccctggtagg cccaaccggc gtgggaaaat ccaccctggc aagcttgata ccgcgctttt
     7501 atgatgtgag cggaggccgc ataacgctgg acggcgtgga tatccgctgc ctggacacga
     7561 aggagctgcg cagtcaaatc agcatggtgc tgcaggacgt ggtcctcttt cacggcagcg
     7621 tccgcgagaa tatcctgttt ggcaacccac aggcgagcga agaagaaatg ctcaccgccg
     7681 caaaaatcgc caatgtgcag gaatttgtct ctgccatgcc ggatgggtac gatacgatga
     7741 ttggcgaacg cggggtaaaa ctctccggcg gacaaaaaca gcgcatctcg attgcccggg
     7801 cgctgctcaa gaacagccca atcctgattc tggacgaagc cacttcctcg gtggataccg
     7861 aaacggaaat gctcatccag gaagctttgg aacacctgat ccagggcaga accaccatca
     7921 tcatcgccca caggctcacg accgtgcgga atgccgacca aatcgccgtg ctggaaggca
     7981 gtaccattac cgaaattgga acccacgccg acttgcttgc ccgggaaggc ctttaccacc
     8041 ggctttatag tatccaacag cgcctgaatt aaggtcggcg ctgagattgg ggcttgtcag
     8101 atcgtcgtga gggagtacca atctacggaa gcgggaaaat ggtctcagga ttgcgttcca
     8161 atacaacggg ctggatttgc ccggcgctca ggttggtcag ttgttgtaag aaagcatcca
     8221 gttcttcatc gcgaaatcgc agtatcagcg tcacatctgc cagaaactga ctttccagct
     8281 gctcgccgcc gtgcgcggct gccaggcgct ggacttgttc gaacagcgga tagggaagct
     8341 gaacaccaat cgtggtggtc gggactttaa tagcccggcg caccagcttg agcacctcgc
     8401 gaaccgcgtc cccgtaagct ttgaccagac cgccggtgcc cagcagcgtc ccgccaaagt
     8461 aacgcgtcac caccacggcg gcatccccca gcccactgcc ctgcagaaca gccagcgccg
     8521 gcctgccggc tgtgccggaa ggctcgccgt catccgagca gtgtgtgatg acgctgttgc
     8581 cgtgcccgat tacgaaggcg ggcacgtggt gcgtggcatc tgggaagcgc tgacggatgc
     8641 tcttgtggaa agcgcgcgct tcctcgacgc tgaaggccgg gctcaggctg gcgatgaagc
     8701 gggaattcac cacgaggatc tcgctctcgg ccggcgcggc agggatgagg taagggcttg
     8761 tgggcatggg ct
//
LOCUS       DAOQAD010000076         8734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_9567_length_8734_cov_5.884088, whole genome shotgun sequence.
ACCESSION   DAOQAD010000076 DAOQAD010000000
VERSION     DAOQAD010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9567_length_8734_cov_5.884088"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(414..3971)
                     /locus_tag="PKL60_06640"
     CDS             complement(414..3971)
                     /locus_tag="PKL60_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNW95846.1"
                     /translation="MPRFIRFFLIFGLLTLLFSLVVPGSAAPRNASNPSVRVLRSSAE
                     GVSFEVVLSEDALTFESLVVEGVEYTSVAFADAALTSVPGQPELPFLSEMLGAPFGAE
                     VQLSISASAPVRVALDTPLLPAPRQNVPTNWEAIAATAPLAPQVKESRDPDPSIYASA
                     SPFPGELAAVRNDGTIRQQRVVSIGIFPVQYQPAENALLLYETVRVNISFGGVDIDSL
                     SAARTESNVYEQLLSESLINYTQAKAMRRAVQPETTPFSAAQLNAAGGKNWTPPSPGW
                     RISTGAEGFYRISAGELSAVGVPVESIPPENYHMFHLGEEIAIQVSPQDDILFYAEAL
                     KSKYTADNIYWLSYDGTPGLRMQTRDAAPGSEELASSFERAEHYEIDANYRSRSPAVD
                     DYDHYFWKYVLRSGTSYVDWVRPFNLNRWTGGEISLNLPLIGYNDALEVNPDHHVGIL
                     VNATQVYEGTWDAFTALEADLTVPAELLIQGTNTLTVTALDTGYAADYFLIDWLDLAY
                     SGDFQVSGNVLKFSQSSAGDWRFELTGFTSNDALVFDISNPLSPAELTGGTYTGEGPY
                     ALQFHDEVLDTAEYLALTPDKLLTIKSIISDTSSNLAAPTRQADYLVISHANFLAAAQ
                     PLAGLREADGLTTVIIDVQDVYDEFSYGIIDPAAIRSFIAFAYAQWQAPAPTYVLLVG
                     DGTYDPKNNEGNGRTSFLPPYLAFVDSTLGETAADNRYVSIVGADAMPDLMLGRLSVN
                     EASEVTAYLDKLMSYAANPPSGEMLRRILAITSRYEVNAQFPVISDELLRDEFPGEPY
                     FAQKVYWKWTHNDLAQARADIQSGINEGRLLVNYIGHGYYAGWGDTNEYLFLASDISS
                     LQNQDKYPFVIAMTCMDGLYTYPSPINSGYEAMAEISTRTAQKGSIASWSPTGWGMVA
                     GHDLLNRGALHAIFETGTSELGGITQAGLMRVWSSGENLDLLETYLLFGDPAIRLPRA
                     FTAVPDLYTGTEDTQLVVDADSGVLSNDINPEGAELIASLVEDALHGALTLNADGSFS
                     YSPDLDYCGDDSFSYKITHGSLDSNTVQVTLHVIGVNDHAPVASEQDVTAYLNTPVPI
                     TLEYFDSDECGGISTCMLDTARANGAKFSYTFEIVTAPGHGTLSGTGPIFEYTPTTNY
                     VGPDAFTFKVNDGLFDSNVAQITITVLADHNIFLPILVK"
     gene            complement(4039..4893)
                     /locus_tag="PKL60_06645"
     CDS             complement(4039..4893)
                     /locus_tag="PKL60_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95847.1"
                     /translation="MRICIAGFCIHIQEGKYHLSVEGLPCHYKFLSTLEQPSSADVLS
                     LRFAEDPTEIPNRPANPLCQNEIWELWFDEAGNYVFTQPAQAPQRWVLIKPDFTEGVI
                     YGDFSALAGAPFYPLEYLDIVLYSNWLALRGDLILHASGVILRDEAVIFLGNSGAGKS
                     TLAADLKTAHNLTILGEDQIILRKIGGVFTAFGTPWHEAPERCDSRGAPLGKLFFLDR
                     AAEQTLTPLDGFEASVRIMTTAFIPYYLPARVQAILDTLSSLPAQARAFLLSYQRGSD
                     VLELIHSA"
     gene            complement(5175..6287)
                     /locus_tag="PKL60_06650"
     CDS             complement(5175..6287)
                     /locus_tag="PKL60_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNW95848.1"
                     /translation="MMDCLNHSDLDMDDWGELLLQPLHGQRFPLAAEFEITERCNLGC
                     VHCFINQPAGSSASIQREMSTDQVRQVLDQIAEAGCLHLLLTGGEPLLRPDFAEIYLY
                     ARQKGMLVMLFTNATMMSPAVIETLKQMPPILVEVSVYGATKETYEAVTRVPGSWERF
                     TNGLKLLQESGLPLALKSVVLSKNKSDLPAIKKMAQDMGVNFRYDGSVFPRLDGSDAP
                     YWDRLDIEEMLALDREDPERMHAWTLQYELSQKLGLRSEKYVYSCGAGKRSFHIDCQG
                     RLAMCMMARTPSYNILELGFPRAWEELGKETQLERTLQVPCLDCGASGICIQCPGWSQ
                     LVYGDNESVVGFICKLNKAREHEICYNKKVIEEHIL"
     gene            complement(6287..6577)
                     /locus_tag="PKL60_06655"
     CDS             complement(6287..6577)
                     /locus_tag="PKL60_06655"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNW95849.1"
                     /translation="MKDQDELFIRNPDFVFRRIINETILVPVRRNLAEIDCIYTLNEL
                     GALLWEKLSAPASKTELCQVILDTYDTSEEAACADLNHFLDELQSIGALQKV"
     gene            complement(6594..7100)
                     /locus_tag="PKL60_06660"
     CDS             complement(6594..7100)
                     /locus_tag="PKL60_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HNW95850.1"
                     /translation="MMSNSALLRGYQCPPDVFAELCVSLLQSGTSIRFIAQGDSMLPS
                     LRSGDTLICEPVEGRQLRVGDVVFFITADGQGIFHRIIRTRRQNASPFFLVQADNALK
                     PDGWKSAGEILGRVNHITRASGTKEDMGTFKMSLLNRWAAFRSRSKLNRFRRLRAWEH
                     GFIQALGR"
     gene            complement(7222..>8734)
                     /locus_tag="PKL60_06665"
     CDS             complement(7222..>8734)
                     /locus_tag="PKL60_06665"
                     /inference="COORDINATES: protein motif:HMM:NF038131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor K domain-containing protein"
                     /protein_id="HNW95851.1"
                     /translation="DVIGVINNVNYGPGGEDERYVRNAVVCGGDVDGDGTFAEWTRTT
                     TIVWTGLGSHTSTCGTNAPNLYISEYVFNVRTFFPEEIAVEVFNKTGADVSTTGWALL
                     MFTADNDFDIIPLTSGRTIANNDVWVMVNDAAADQTTQEDQTFANNDAYRTVVLVKNY
                     ALPVVNGTDFEWPDLVQDDVTTDENQVRYGRPVGDTDCPSGAARFVAQSGFGFEGIES
                     TTLVPTENQPFPIGRFCHYNNPIYSAVNKLETVPLYMNVNDITCPAGWTLDEPGNDLQ
                     FQYQVNLDETTNTTDPCTYGTSSPQWPGGSSSPKVSGDTGPNRNGCADMVNFSNVAGA
                     QSFTCRYDATHAQDYTVSILGFTPTQAGGSCPDVPVGTVTFNRIYTAETAKNCYCVYA
                     AFTRGQITPVVLKYLSAEAAENGILVSWETATEVNNLGFNVFRAELVDGERVRLNPEL
                     ILSEVGPGGMTGATYEFLDETAAVGVTYYYWLEDVPLEAGVTPGIYGPISAVR"
BASE COUNT         1942 a         2363 c         2489 g         1940 t
ORIGIN      
        1 gtgtaatacc cgctgacaga tgcgacggtc tgggggttag cgattggcgc tgctgtcaaa
       61 cttgcggcat tggcggttgt ggcaaaactc ccaagcaacg cgggaggaag tgccagacac
      121 aacaccaaaa tataaataat atattgctta agtttcatgt ttattccttt caaaactatt
      181 tcgatcaagt aagttgggtg gtttttgcgc ttgcgcaaga gagtacagag taaatttcac
      241 tgtaaatgct gcagattata aacagtttat caatttaatc aaggtaatat tacaaaaata
      301 ggcagatttg caaccttcga caagcagaaa ttggaatgcc gccaattttt ggctttaaaa
      361 agcgctgccc acggaatatc accgtgggca gcgctttttt tctgaaggaa aacttacttt
      421 actagaattg ggaggaaaat attatgatct gccaaaactg tgattgtgat ttgggcaaca
      481 ttgctgtcga acaaaccgtc attgactttg aaggtgaaag catccggtcc gacgtaattt
      541 gtcgtgggag tatattcaaa aattggtcct gtgccgctta aggtaccatg cccaggcgcc
      601 gtcacaattt cgaaggtgta actgaattta gcgccattcg cccgcgctgt atcgagcatg
      661 caggttgaaa tccctccgca ctcgtctgaa tcgaagtact ccagcgtgat tgggaccggg
      721 gtgttgaggt atgccgtcac atcctgctca gaagcgaccg gcgcatggtc gttcacgccg
      781 ataacatgca aagtgacctg aacggtgttg gaatccagcg acccatgggt aatcttgtag
      841 ctgaagctgt cgtcaccgca ataatccagg tctggtgaat aactaaagga accgtccgcg
      901 ttcaaggtca gcgcgccatg cagggcgtcc tcaacaaggc tggcgataag ctcagcgcct
      961 tcaggattga tgtcgttgct aagcacgccg ctatccgcat ccaccacaag ctgcgtgtcc
     1021 tctgtgccag tgtagaggtc cggcactgca gtgaaagcgc gcggcagccg gatggccggg
     1081 tcgccgaaca gaaggtaggt ttccaacagg tccaggtttt cgccgctgct ccagacgcgc
     1141 atcaggccag cttgcgtgat accgcccagc tcggaggtac cggtttcaaa gatggcgtgg
     1201 agtgccccgc ggttcaataa gtcatgcccc gcaaccatgc cccatccggt tggagaccaa
     1261 ctggcgatcg agcctttttg cgccgtccgg gtgctaattt ccgccatagc ctcatagccg
     1321 gagttgattg gactcggata cgtgtacagg ccgtccatgc aagtcatggc tatgacaaag
     1381 gggtacttgt cctggttctg cagagaactg atatctgagg caaggaacag atactcgttt
     1441 gtgtctcccc aaccggcata gtacccatgc ccgatgtagt ttaccaacag gcggccttcg
     1501 ttgatgccgc tctgaatgtc cgcgcgcgcc tgagcgaggt cgttgtgggt ccatttccag
     1561 taaactttct gggcaaagta cggttcgcct gggaattcat cccgcaggag ttcgtctgaa
     1621 atgaccggga attgggcgtt gacttcatag cggctggtaa ttgccaggat gcggcgcagc
     1681 atctctccag agggagggtt ggcggcgtag ctcatgagct tatcgagata cgctgtgacc
     1741 tcagaggcct cattcacgga taatctgccg agcatcaaat caggcatggc gtccgctccg
     1801 acaatcgaga cgtaacggtt atccgcagcg gtttcgccaa gagttgaatc cacaaatgcc
     1861 agatagggcg ggaggaaact tgtgcggccg ttgccctcgt tatttttcgg gtcataagtt
     1921 ccgtcgccca ccagaagaac ataagttggg gctggcgctt gccactgcgc gtaggcaaag
     1981 gcgataaaac ttcgaatcgc ggctgggtcg atgattccgt aggaaaattc atcataaaca
     2041 tcctgcacgt cgatgatcac agtagtcaat ccgtcagcct cacggagtcc ggctaacggc
     2101 tgcgcggctg ccaggaaatt ggcatgcgag ataacgagat aatctgcctg ccgggtcggg
     2161 gcagcaagat ttgaggatgt gtctgagatg atgctcttaa tcgtcaacag cttatccggc
     2221 gtgagagcga gatattccgc cgtatccaaa acttcatcgt ggaactgcag cgcatatgga
     2281 ccctcgccgg tataggtacc gcctgttagt tccgccggcg agagggggtt gctaatatcg
     2341 aagactaagg cgtcgttgct cgtaaagccc gtcagttcaa accgccagtc gcccgcggat
     2401 gattgcgaga acttcaaaac attgccgctc acctggaaat caccggaata ggcaaggtcc
     2461 agccagtcga tcaagaaata atcagctgcg taaccagtat ccagcgcggt cactgtcagg
     2521 gtgttggtgc cctgaatcag cagttcggca ggcactgtca ggtctgcctc gagggctgta
     2581 aaagcatccc aggttccctc gtatacctgg gttgcattga ccagaatgcc cacatggtgg
     2641 tcgggattga cttccaaggc atcgttgtag ccaatcaagg gcaggttgag actgatctcc
     2701 ccgccagtcc accggttaag gttaaacggg cgcacccagt ctacgtagga cgtaccgctg
     2761 cgaagcacat atttccagaa gtaatgatcg taatcatcca cagcgggcga gcgcgaccgg
     2821 taattggcat cgatttcgta gtgctccgca cgctcgaatg agctggcgag ttcctcgctg
     2881 cccggggcgg cgtcgcgcgt ttgcatacgc agccccgggg tgccgtcgta gctcagccaa
     2941 tagatattgt ctgcggtgta cttgcttttc agtgcttcag cgtagaaaag gatgtcatcc
     3001 tgcgggctga cctggatggc aatttcctcc ccaaggtgga acatgtggta gttttccggc
     3061 gggatgcttt ctacgggcac acccaccgcg ctcagttccc ccgcgctaat ccggtaaaaa
     3121 ccttccgcgc cggtgctgat gcgccagccc gggctaggtg gggtccaatt tttgccgcca
     3181 gccgcgttga gctgcgcagc gctgaatggc gtggtttcgg gctgaacggc gcggcgcatg
     3241 gctttggctt gcgtgtagtt aatcaggctt tcggaaagca gctgttcgta gacattggat
     3301 tccgttcggg cggctgacag cgaatcaata tccacaccgc caaagcttat gttaacgcgc
     3361 acggtctcgt acagcagcag cgcgttttcg gccggctggt actgtacagg aaatatgccg
     3421 atgctgacca cgcgctgctg gcgtatagtt ccgtcattcc gaaccgccgc caattcgcca
     3481 gggaaggggc tggctgaggc ataaatcgag gggtcaggat cgcggctttc cttcacctgc
     3541 ggcgccagag gggcagtcgc ggcaatcgcc tcccagttcg tcggcacgtt ttggcgcggc
     3601 gcgggcagga ggggggtatc cagcgcaacg cgcaccggcg cgctggcgct gatgctcagc
     3661 tgcacttctg cgccgaaggg cgctcccagc atctcgctca ggaaaggcag ttcgggctgc
     3721 cccggcacac tcgtcagggc cgcgtctgcg aatgcaacgc tggtgtactc aactccttcc
     3781 acgacaagcg attcgaaggt cagagcgtcc tcggagagca cgacctcgaa gctcactcct
     3841 tccgccgagg agcgaagcac acgcaccgag gggttcgagg cattgcgtgg ggcggctgag
     3901 cctggaacaa ccagggagaa aagcagggtt aggagcccga aaataagaaa gaaacggata
     3961 aacctgggca tagtacttct cctgagttct gcaagttgga aaagctgatg ataacgatta
     4021 gcgggtcaaa ctggccgttt atgcgctgtg gattagctcc agtacatccg atccgcgctg
     4081 gtaagagagc aggaacgcgc gcgcttgcgc cggcagggac gaaagcgtat ccaggatggc
     4141 ctgcacgcgc gcgggcagat aatagggaat aaaggcggtg gtcatgatcc gcacggaggc
     4201 ctcaaaccca tccaggggcg tcagggtttg ctccgctgcg cggtcgagga aaaacagctt
     4261 gccaagcggc gcgccgcgcg agtcgcagcg ctcaggagct tcatgccagg gcgtgccaaa
     4321 agcagtaaat acgccgccaa tttttcgcag gatgatttgg tcttccccca ggatggtcag
     4381 attgtgcgcg gttttcaggt ccgctgccag agtggacttg cccgcgccgg aattgccgag
     4441 aaaaatcacg gcttcgtccc gcaggataac cccggaggcg tgtaggatga ggtctccgcg
     4501 cagcgccaac caattggaat agaggacaat gtcgagatat tcgagcggat aaaaaggcgc
     4561 gcccgcaagc gcgctgaaat cgccgtaaat cacgccttcc gtgaaatccg gtttgataag
     4621 cacccaacgc tgcggagcct gcgcgggttg cgtaaaaacg tagttcccgg cttcgtcaaa
     4681 ccacagctcc cagatttcat tttggcagag cgggttggcc ggtctgttcg ggatttcggt
     4741 cgggtcttca gcgaagcgca gactcaggac atccgcagag ctgggctgct ccagcgtgga
     4801 taaaaacttg tagtggcagg gcaagccctc cactgacagg tggtatttac cctcctgaat
     4861 atggatgcaa aacccggcga tacaaatgcg catatgctgt gattacgttc cagaagagat
     4921 tggattagct gtaagaatca aatgagtacc cgcacgtgca cacgtcaagc gcgaatactc
     4981 atgggaccat aaaaacacgc ttgctaatta agtgggctaa acctgtctgg tagagcagcc
     5041 gccaacctga gcagtgcagc ccaactcgtt cttagggaat aaattcgggc tgctgtgaca
     5101 gaagcccaaa atcgcgttct ttggtttcag gataaccttg ctgacagcag gcggtgtata
     5161 tggcttcttg ttcatcataa aatatgctcc tcaatgacct tcttattata acatatttcg
     5221 tgctcgcggg ctttattaag tttgcagata aagccaacta cactttcgtt atccccataa
     5281 accagctggg accagccggg gcattgaata caaatcccgg aagcgccgca gtccaggcaa
     5341 ggcacctgca gcgtgcgctc cagttgggtt tccttcccga gctcttccca ggcccgggga
     5401 aagcccaatt ccagtatgtt gtacgaaggc gtacgcgcca tcatgcacat tgccagccgt
     5461 ccctggcagt caatatggaa ggagcgcttg ccggcgccgc agctgtacac atatttctcg
     5521 ctgcgcagtc ccagcttctg gctgagctca tattgcagcg tccaggcgtg catacgctcc
     5581 gggtcctccc ggtccagcgc aagcatttcc tcgatatcca ggcggtccca atagggggcg
     5641 tccgaaccgt ccagacgggg gaaaactgac ccgtcgtagc ggaagtttac acccatgtcc
     5701 tgcgccattt tcttgatcgc cggcaagtcc gacttgtttt tggagagcac cacggatttg
     5761 agcgccagcg gcagaccgct ctcctgcaaa agcttcaatc cgtttgtgaa gcgctcccag
     5821 gaaccaggta cgcgcgtgac tgcctcgtag gtttctttgg tcgcgccgta aacactgact
     5881 tcaaccagta ttggcggcat ctgcttgagt gtctcaatca cagccgggct catcatggtg
     5941 gcattggtaa atagcatcac aagcatgccc ttttggcggg cgtacaggta aatttcggcg
     6001 aaatccgggc gcagaagcgg ctctccaccg gtgagcagca agtgcaggca gcccgcttca
     6061 gcgatttgat cgagcacttg cctgacctga tctgtgctca tttcccgctg gatggaagcg
     6121 ctgcttcctg ccggttggtt gatgaaacag tgcacgcagc ccaggttaca gcgctcggtg
     6181 atttcaaatt ccgcggcaag cggaaagcgt tgtccgtgca gcggctggag gaggagttct
     6241 ccccagtcgt ccatatccag atcagagtga tttaagcagt ccatcatcag actttctgca
     6301 gcgcgccgat cgactgcagc tcatccaaaa agtgattcag gtccgcgcag gcagcttctt
     6361 cactggtgtc gtaggtatcc agaatcacct ggcaaagttc agttttcgag gctggggcgc
     6421 taagtttttc ccacaggagc gcgccgagct cgttgagcgt gtagatacag tctatttcgg
     6481 cgaggttgcg gcgaaccggc acaagaatgg tttcgttgat aatccgccga aaaacaaaat
     6541 ccgggttgcg aatgaaaagt tcgtcctggt ctttcaaaat atctccgtgt taattatctg
     6601 ccaagggcct gaatgaaacc atgctcccat gcgcgcaggc gccggaagcg gttaagtttg
     6661 cttcgcgagc ggaaagccgc ccacctgttc aacaggctca ttttaaaggt gcccatatcc
     6721 tctttagtgc cgcttgcccg ggtgatatgg ttcactctgc ccaggatttc cccggcgctt
     6781 ttccatccgt ccggttttaa ggcgttatcc gcctgcacca ggaagaacgg gctcgcattt
     6841 tgtcttcgcg tacgaatgat cctatgaaaa ataccctgac catccgcagt aatgaagaag
     6901 acgacgtccc cgactctcag ctggcggcct tcaacgggtt cgcaaatgag tgtatccccg
     6961 ctgcgcaggc tgggcagcat gctgtcccct tgggcgatga aacggatgga agtaccggat
     7021 tggagcagac tgacgcacag ctccgcgaaa acatccggag ggcattgata tccccgcagg
     7081 agtgcgctgt tgctcatcat tcccctttaa tcgcccgcca gacagcacgc gctgagattg
     7141 gaaacagccg cgccgccacc cgaccgccag aaatgcggtt agtcagtggc gcggctgatc
     7201 gcgcgtgcgc agcgttgagg gctacctcac cgcgctgata ggtccgtaaa taccgggtgt
     7261 cacgcctgct tccagcggga cgtcttccag ccagtagtag taagtcacgc cgaccgcggc
     7321 ggtctcatcc aggaactcat aggtcgcgcc ggtcatcccg ccggggccaa cctcgctcag
     7381 gatcagttca ggattaagcc tgacgcgttc gccgtctacc agttcggcgc ggaacacgtt
     7441 gaagccgaga ttgttgactt cggtcgccgt ctcccaggag accaggatgc cgttctcggc
     7501 cgcttcggca gagaggtact tcagcacaac cggggtaatt tgccccctcg tgaaagccgc
     7561 gtacacgcag taacagttct tggcagtttc ggcggtgtaa atgcggttga acgtaacggt
     7621 gccgaccggg acatccgggc aggagccgcc tgcctgcgta ggtgtgaagc cgaggatgga
     7681 cactgtgtaa tcctgggcgt gggtggcatc gtagcggcag gtgaaggatt gcgcgccggc
     7741 aacgttgctg aagttaacca tatccgcgca gccgttgcgg ttggggccgg tatccccgct
     7801 aaccttgggg ctgctgctgc cgcccggcca ctgcggcgag cttgtgccgt aagtgcaggg
     7861 atcggtggtg ttagtggttt catccagatt gacctggtat tggaactgga ggtcgttacc
     7921 cggttcatcc agcgtccatc cagctgggca ggtgatatca ttcacattca tataaagagg
     7981 aactgtttcg agcttgttta cggctgaata gatcgggttg ttatagtggc agaaacgccc
     8041 gattgggaaa ggctggtttt cggttgggac taaagtggtg ctttcgatac cttcaaagcc
     8101 gaaaccgctt tgcgccacaa aacgcgctgc gccacttggg caatctgtgt cccctactgg
     8161 cctgccgtag cgcacctggt tctcatcggt cgtaacatcg tcctggacta aatctggcca
     8221 ttcaaagtcc gttccattga ccacagggag cgcgtaattc ttgacgagca cgaccgtgcg
     8281 gtaagcgtcg ttattggcga aggtctgatc ctcttgagtg gtttgatcgg ctgccgcatc
     8341 attgaccatc acccagacgt cgttattggc gatcgtccgg cccgaggtga gcggaatgat
     8401 gtcgaaatca ttatccgcgg tgaacatcag cagcgcccaa ccagtggtgg atacatcagc
     8461 accggttttg ttgaatacct ccacagcaat ttcctctgga aaaaaagtac gtacgttaaa
     8521 tacatattca gagatgtaaa ggtttggcgc gttggtgcca caagtgctgg tgtgggaacc
     8581 taagcctgtc cagacaattg tggtcgtgcg ggtccattca gcaaaggttc catcgccgtc
     8641 gacatccccg ccgcacacta cagcgttacg cacgtagcgc tcatcctcgc cgcccgggcc
     8701 gtaattcaca ttattgatca cgccgatgac atcc
//
LOCUS       DAOQAD010000077         8728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_9574_length_8728_cov_5.522195, whole genome shotgun sequence.
ACCESSION   DAOQAD010000077 DAOQAD010000000
VERSION     DAOQAD010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9574_length_8728_cov_5.522195"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKL60_06670"
     CDS             <1..856
                     /locus_tag="PKL60_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNW95852.1"
                     /translation="VQTAPAVRVGLGEELGFDAAPSTPEELQAQMLPAAAEKLLDKDD
                     TNNGAGGLWISDSAQAPLAWYQAFGGQITAKENALSFNNSAFQDAITFLKGAYAQDAA
                     WVGLQKTPYDYFARRQALAYEGTLDDLLAQEGTIARSGSSDTFVTLPYPTADGSGSIS
                     LETLSVGIRAGSNAEQLGAWLFARWLLSAPAQEVLVSVSGFWPAAGDPGNIAPDYTRQ
                     HPAWASALRTDTRLQLAPERENWAFSRLVLQDALRRAYQLDLEYIPTVLETLDATLAE
                     LAGAGSGD"
     gene            846..1289
                     /gene="rnhA"
                     /locus_tag="PKL60_06675"
     CDS             846..1289
                     /gene="rnhA"
                     /locus_tag="PKL60_06675"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNW95853.1"
                     /translation="MATKLPEVTVYCDGACSGNPGPGGWAAVLLFGQGEERVLKGGEK
                     DTTNNRMELTAALQALRALREPCAVTFVTDSSYLMNGATIWLPGWKQRNWKRKEGALK
                     NIDLWQALDRELQRHQVRWEWVRGHSGNRCNERADREARSEIPNA"
     gene            1405..2850
                     /locus_tag="PKL60_06680"
     CDS             1405..2850
                     /locus_tag="PKL60_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HNW95854.1"
                     /translation="MNTYINPLDQSYLNFTRARTAHWDAIAARSPKKINWSAYYHRRL
                     KHVYRHIIPSGASVLDLGCGKGDLLASVQPAYGVGIDFSAEMVRAAQAAYPDLHFIQA
                     DAHALPALDRQFDYVILSDLVNDVWDVQQVLNQLRPLCSPRTRVVLNFYNRGWEAALK
                     LAQALGTAKPLLDQNWLTQTDMKNLLNLGDFEVVRRWTEVLFPFNIPLISPFLNRVAA
                     RLWPLKHFCMTNMMIARPRCSLKAQSASVSVVVAARNEAGNIQKIFERVPEMGSGTEI
                     VFVEGHSKDDTWEVIEQTIKESPRKAVLLKQPGKGKGDAVRAGFQAASGDMLMILDAD
                     MTVPPEDLPRFYDALVQNRGEFINGVRLVYPMEEEAMRFLNSLGNKFFSVTFSWLLGQ
                     SIKDTLCGTKVLWRADYEKIAQNRAFFGDFDPFGDFDLIFGAAKQNLKLVDMPIRYRE
                     RTYGTTNIQRWRHGVLLLRMLWVAAVRLKFV"
     gene            complement(2851..4851)
                     /locus_tag="PKL60_06685"
     CDS             complement(2851..4851)
                     /locus_tag="PKL60_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95855.1"
                     /translation="MEKFIPLLSVLLWVFLWSAGGFLILINTIKTQRHETTLFGLGLG
                     LVLQVWLANFTGRLLPPVQAFWLSAGLVFALGLVLTIILKSWKDARQNLYFPPGQWLA
                     FFVLLYAFFMIGRGLAIFDDYQNLPVTSQLASGVIPPLFPLNPTLSFDYHYLLLLNAA
                     QWMRVGGFLPWTALDFARALCFSLAVLYAYSLGERLTRSKAGAWLTAIFTAFAGGMRW
                     VLLFLPGRLLSAISERVTMIGSGLATDSSLKFALSKPWAIEGGGPFPFPFAYGSGFHT
                     PAVMEHDGTGLMGLVIALLIILFFERWKRKGGLAVVAALLAAQALTDEIWFVFFFFAL
                     IVVLIVQLFRPGKTPRSQIWLSLLVLGLIPLVFALLQGGVLTGAAAGIITQLAGGPAA
                     VQSQYFSLGFHLRFPPAFISSHLGTLSLTNWAQALAAIAEVGPIFLLLPLFILWGLKG
                     FRANRLIYPILALGAAASFLTIFMEYEGAAGISATKRLTLYSSVLLTLFTVPILWYWL
                     RRKKQVLRFLAIGLCALSMLGGVVYFAVESAAAQVPQESYFLDKLDTAFYQKYWNALE
                     PGAMVFDLVPSRAATVFARPLQSNQSWYAELPEYSALASSMDPQAIRAAGYQYIYLGS
                     SEWDDFSAAVQAGLSAPCVHVVDEVNGANGTFRRLLDISECP"
     gene            complement(5171..7591)
                     /locus_tag="PKL60_06690"
     CDS             complement(5171..7591)
                     /locus_tag="PKL60_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNW95856.1"
                     /translation="MPDKQQVTLPILGMTCANCVAAVERGLSKVQGVDTAVVNLSSER
                     ASVSYDPQKTDVPALVEQIRQSGYDVALGEASLGVIGLSDPADGARLEKALNTVEGVL
                     FSQANLASERLVVRYVPTIISQSEIRQAVLRAGFRLAEERTLTDDPEAAARQKETAHQ
                     KRLLIIALIFTVPLFILSMGRDFGLLPMAVAHAPWMDWLFLALATPVQFYVGASYYKN
                     GYKSLRNKSANMDVLVALGTSAAYFYSIFVLLGAVPGHGYFETSAVIITLVRLGKFLE
                     ARAKGGASEAIRKLLFLQPKKATVIRDGMETEIPADEVLIGDVIVVRPGEKIPVDGSV
                     LEGSSSVDESMLTGESLPVEKSAGSPVYGATLNKNGRLVFEATKIGRDTVLSQIIQLV
                     ENAQASKAPIQKLADKISAVFVPIVIAIAALTFLIWYFLVPLPANSEISLLARALINT
                     VAVLVIACPCAMGLATPTAVMAGTGRGAELGILVKTSESLEIAGQIDTIILDKTGTLT
                     RGQPAVTEIVPLAEGWTEEQALALAASVEAGSEHPLGEAVTAAAKARALTLQSVERFR
                     SSSGKGVSAEVDRKKVSVGSRRYLEGLQVDTSRAESVQAELEQQARTVVLLAADGELL
                     GAFGIMDTLKENSVSAVAELHKLGLKVAMLTGDNARTARAVASQAGIDEVYADLLPRD
                     KAGQVQALQASGHKVAMVGDGINDAPALAQADVGIAIGTGTDVAIAAAPIVSISGDLQ
                     AVPLAVRLSRKTLKTIRQNLFWAFFYNVILIPAAAAGFLNPMLAAGAMSFSSIFVITN
                     SLRLKRFK"
     gene            complement(7592..7798)
                     /locus_tag="PKL60_06695"
     CDS             complement(7592..7798)
                     /locus_tag="PKL60_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNW95857.1"
                     /translation="MTSKTYFIPNIHCAHCVMHIKNRLNEIEGVESVEGNVQTREVEV
                     EFSSPATEEAILQALAEINYPAEV"
     gene            7877..8674
                     /locus_tag="PKL60_06700"
     CDS             7877..8674
                     /locus_tag="PKL60_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNW95858.1"
                     /translation="MDFLREAQGFILDMDGTFFLGGQLLPGALEFLALLNRLELPFSF
                     LTNNTSRSRADYVKKLVGLGVGEEDARVFTAGDAVIRYLRKHFDGQGVCLIGTRSLRE
                     DFRSAGVRLEYRKPAAVVLGYDTELTYAKLVRLCTFVRQGLPYFATHPDVNCPTPNGP
                     IPDIGATMALVEKSTGRLPDKVFGKPDRNLIEQLAGSLNLRPQSLVMVGDRLYTDIAM
                     GTTAGVGTVLVLSGETKREDLPGAAFQPDFVCEDLAELGRMLEKVRG"
BASE COUNT         1804 a         2528 c         2695 g         1701 t
ORIGIN      
        1 tgttcagaca gctcctgctg ttcgggttgg tttgggcgag gaactgggtt ttgacgccgc
       61 gccgagcacc ccggaggaac tgcaagcgca gatgctgcct gccgcggctg aaaagctgct
      121 ggataaggac gacaccaaca acggcgcggg cggtttatgg atttccgatt ctgcgcaagc
      181 gccgctggcg tggtaccagg cctttggcgg ccagataacc gcgaaagaaa atgcgctcag
      241 cttcaacaac agcgcgttcc aggatgccat cactttcctc aagggcgcgt acgcgcagga
      301 cgccgcctgg gtcgggctgc agaaaacgcc ttacgactac ttcgcgcgcc gacaggcttt
      361 agcctatgaa ggcacgctgg acgacttgct cgcgcaggaa gggactatcg cgcgctcggg
      421 cagcagcgac acgttcgtca cactcccgta tcccactgcg gacggcagcg gcagcatatc
      481 gcttgagacg ctttcggtag gaatccgcgc cggcagcaat gcagagcagc tgggggcctg
      541 gctctttgcg cgctggctcc tgagcgcgcc agcccaggag gttctggtaa gcgtcagcgg
      601 cttttggccg gcggccggag accccgggaa tatcgctccg gactataccc gccaacatcc
      661 ggcctgggcc agcgccctgc gaaccgacac gcgcctgcag ttggctccag aacgcgaaaa
      721 ttgggctttc tcccggcttg tcctgcagga tgcactgcgc agagcgtatc aacttgatct
      781 cgagtatatt cccacggttt tggaaaccct ggacgcgacg ctggcggaac tggcaggagc
      841 gggcagtggc gactaagctg cccgaagtca cggtgtactg cgacggagct tgcagcggca
      901 accccgggcc gggcggttgg gcggcggtgc tcctgttcgg gcagggcgaa gagcgcgtgc
      961 tgaagggcgg cgagaaagac acgaccaaca accgcatgga actgacagcc gcgctgcaag
     1021 cgctgcgcgc gctccgtgaa ccctgcgctg taacgttcgt aaccgattcc agctatctga
     1081 tgaacggcgc caccatctgg ctgccgggct ggaagcagcg caactggaag cgcaaggaag
     1141 gggccctgaa aaatatcgac ttgtggcagg ctcttgaccg cgaattgcag cgccaccagg
     1201 tgcgttggga atgggtgcgc gggcactcgg gcaaccgctg caacgaacgc gcggacaggg
     1261 aagcgcgaag cgaaataccc aacgcttagc tttctgccgg atttagcctg ctggcctcat
     1321 tcaggtttcg gtaagctctg ctttttgtta tactttgggg gtaatcaggg aaatcaatct
     1381 gcgaaaagcg gccgagttga gcgcgtgaac acctacatca atccattgga tcagtcttat
     1441 ctgaatttca cccgggcgcg gactgcgcat tgggacgcca tcgctgcaag gtccccgaaa
     1501 aaaatcaact ggagtgcgta ttatcaccgc cggctcaagc acgtttaccg gcatatcatt
     1561 ccgtccggcg cttcggtgtt ggacctgggc tgcggcaaag gcgatttgct cgcctcggtt
     1621 cagcctgctt atggtgtcgg aattgacttt tccgcggaaa tggtgcgcgc cgcgcaggca
     1681 gcttatcctg atttgcattt catccaggca gacgcgcacg cactgcccgc tttggaccgg
     1741 caatttgatt acgttatcct ttcagacctg gtcaatgatg tctgggatgt gcagcaggtg
     1801 cttaatcaac tgcggccctt gtgcagtccg cgcacgcgcg tggtgctgaa cttttacaac
     1861 cgcggctggg aagcggcgct caaactggcc caagcgctgg gcaccgctaa gcctctgctg
     1921 gaccagaact ggctgacgca aactgacatg aaaaacctgc tcaaccttgg ggattttgag
     1981 gtcgtgcggc gctggacgga agtgctgttc ccctttaata ttccgctaat cagcccattc
     2041 ctcaaccggg tagccgcgcg cctgtggcct ctaaagcact tctgcatgac caatatgatg
     2101 attgccagac cgcgatgcag cctgaaagcg cagtcggctt ctgtctccgt ggtcgttgcc
     2161 gcgcgtaatg aagccggtaa tatccagaaa atcttcgagc gcgtgcccga aatgggcagc
     2221 ggaaccgaaa tcgtgtttgt ggaaggacat tcaaaggatg acacctggga agtcatcgag
     2281 cagacaataa aggaatcgcc gcgcaaggct gtcctgctga agcagcccgg caaaggcaaa
     2341 ggcgatgccg tccgggcggg ttttcaagcc gccagtgggg atatgctcat gatcttggat
     2401 gctgatatga ccgtgccgcc tgaggacctg ccgcgctttt acgacgcgtt ggtgcagaat
     2461 cgcggcgagt tcatcaacgg agtgcgcctg gtttacccga tggaggagga agccatgcgc
     2521 tttctcaaca gcctggggaa taaattcttc agcgtgactt tttcctggct gctcgggcag
     2581 agcatcaagg ataccttgtg cggtaccaag gtgctctggc gcgcggacta cgaaaaaatc
     2641 gcgcagaacc gggcgttctt tggcgacttt gacccctttg gggatttcga tttgattttc
     2701 ggcgcggcga aacagaacct gaagctggtg gacatgccta ttcgctaccg cgaacgcacc
     2761 tacggtacga ccaatatcca gcgctggcgg cacggagtgc tgctgctgcg catgctgtgg
     2821 gtggcagctg tgcgcctgaa gttcgtgtga ttacggacac tccgaaatat ccagcaggcg
     2881 tcggaaagtt ccgttggcgc cattcacctc atccacgaca tgcacgcacg gcgctgaaag
     2941 ccccgcctgt accgccgccg agaagtcgtc ccattcgctg ctgcccagat aaatgtactg
     3001 gtagcctgcc gcccggatag cctgagggtc catgctgctg gcgagcgcgc tgtattcggg
     3061 cagctcggcg taccagctct ggttggactg cagcgggcgg gcgaagaccg tcgccgcccg
     3121 ggagggaacc aggtcaaaga ccatcgcacc gggctcgagc gcgttccaat atttctgata
     3181 gaaggctgtg tccagcttat ccaggaagta actctcttgc ggcacctgcg ccgcggcaga
     3241 ctccaccgcg aagtacacca ctccgcccag catgctgagc gcgcacaagc caatcgcgag
     3301 gaatcgcagg acttgctttt tgcgccgcag ccaataccac aggatgggca cagtgaagag
     3361 cgtgagcaaa acggaagaat agagcgtcag gcgttttgtg gcggagatgc cggctgcgcc
     3421 ctcgtattcc ataaaaatcg tcagaaagct ggcggcggcg cccagcgcga gaatcggata
     3481 aatcagccgg ttggcgcgga agcccttgag cccccagagg ataaagagcg gcaggagcag
     3541 aaaaataggc ccgacttcag caatggcggc cagcgcctga gcccagttgg tcagcgagag
     3601 cgtgcccaga tgggaggaga tgaacgccgg cggaaagcgc aggtgaaaac ccagactgaa
     3661 atattggctt tgaacagccg ccgggccgcc cgcaagctgc gtgattattc cagccgcggc
     3721 gcccgtgagc accccgcctt gcagcagcgc gaacacaagc ggtatcaacc ccaggaccag
     3781 cagcgaaagc caaatctggc tgcgcggggt tttccccgga cggaaaagct gcacaatcag
     3841 gaccacaata agcgcgaaga agaaaaacac gaaccagatt tcatcggtaa gggcttgcgc
     3901 tgccagcaga gcggccacca ccgccagccc gcctttgcgc ttccaacgct cgaaaaacag
     3961 gatgatcagc agcgcgatta cgagccccat caagccggtg ccgtcgtgct ccatgaccgc
     4021 gggcgtgtgg aaaccgctcc catacgcgaa gggaaagggg aaagggccgc cgccttcaat
     4081 cgcccagggc ttggagagcg cgaatttcag gctgctgtcc gtcgccaggc ctgagccaat
     4141 catcgtcacg cgttcggaaa tcgcggagag cagccttccg ggcaggaaca acagcaccca
     4201 gcgcatccca cctgcgaagg ctgtaaagat tgcagtgagc cacgcgcccg ctttgctgcg
     4261 ggtcaggcgt tcgcccagcg agtaggcgta cagaaccgcc aggctgaagc acagcgcgcg
     4321 ggcaaagtcc aaagcggtcc aaggcaaaaa gccgcccacg cgcatccatt gggcggcatt
     4381 cagcaggagc aggtagtggt agtcaaagct gagggtgggg ttcagcggaa aaagcggcgg
     4441 gatgacaccg gaagcgagct gcgaggtgac cggcaggttc tggtaatcgt cgaagatggc
     4501 cagcccgcgt ccaatcatga agaaggcgta taagagcacg aaaaaagcca gccactgccc
     4561 aggcgggaaa tacagatttt ggcgtgcgtc cttccaggat ttcaggatga tggtcaggac
     4621 cagccctagc gcaaagacga gccccgcgct aagccaaaag gcctggacag gcggcagcag
     4681 cctgcccgta aagttggcca gccatacctg cagcaccaga cccagaccca gcccgaacag
     4741 cgtggtttcg tgccgttggg ttttaatggt attgatgaga atcaggaatc cgccggcgga
     4801 ccaaaggaaa acccagagca ggacggataa aagcggaata aacttttcca tagcagacgc
     4861 ggcctcccgg ggtagcgtgg tttatgctga ttatactttg ccctcaggat ctgttgagct
     4921 tctcgcagag ctgtcagtgg gttttatccg gtcagcttcc ggcctgaaca aggcagagtt
     4981 agcgtgtgtc ttttgggatt agcgcccaag gcggctgcgt acggaaaaaa gtcgggcgtg
     5041 ctgacccgac tctatgctga acctgttctg ctttttgacg cggcatgaaa aggaatggca
     5101 gcagcatttg cctgcttttc atgccgggcg gagtgcgtac gtaacgcgta tagcgccccc
     5161 gccccgatgc ttacttgaag cgcttgaggc gcaggctgtt ggtaatcacg aaaatgctgc
     5221 taaacgacat cgctccggct gccagcatag ggttgagaaa gcccgcggct gccgccggaa
     5281 tgaggatgac gttgtagaaa aacgcccaga acaggttctg cctgatggtc ttgagcgtct
     5341 tgcgggaaag gcgcactgcc aatggaacag cctgcaaatc gccgctgatg gagacgatag
     5401 gcgcggccgc gatggccaca tctgttccgg tgccaatggc gatgcccacg tccgcctgcg
     5461 ccagcgccgg cgcgtcgttg atgccgtcgc caaccatagc gaccttatgc ccgctggcct
     5521 gcagcgcttg cacctgcccg gctttatccc tggggagcag gtcggcgtag acctcgtcga
     5581 ttccggcctg gctggcgact gcccgggcgg ttcgggcgtt atcgccggtg agcatggcta
     5641 cttttaggcc cagcttgtgt agctccgcca ccgcgctgac cgaattttct ttcagcgtgt
     5701 ccataatccc gaaggctccc agcaattcgc cgtcggctgc caacagcacg accgtgcggg
     5761 cttgctgttc gagctccgcc tggacgcttt ccgcgcggct ggtatccacc tgtaagcctt
     5821 ccaggtagcg ccggcttccg accgagacct tttttctgtc cacttccgcc gagacgccct
     5881 tgccggaaga agacctgaag cgctccacgc tctgcaatgt gagcgcgcgc gctttggcgg
     5941 cggcggtgac agcctcgcca agcgggtgct cgctgccggc ttccacggaa gctgccagag
     6001 ccaaggcctg ttcttccgtc cacccctcgg ccaggggcac aatttcggtc acagccggct
     6061 gcccacgcgt gagcgtgccg gttttatcca ggatgatggt gtcaatctgg ccggcaattt
     6121 ccaggctctc gctggttttc accagaatgc caagttccgc gccgcgtccg gtgcccgcca
     6181 tcacggcggt cggggttgcc agacccatgg cgcacgggca ggcaatcacc agtaccgcga
     6241 cggtattgat gagcgcgcgc gccagcaggg aaatttcaga attcgccggc agcggcacca
     6301 gaaaatacca tatcaggaaa gtcagcgcgg cgatggcgat gactatcggg acaaaaaccg
     6361 cggatatttt atccgcaagc ttctggatgg gggccttgct ggcctgggca ttctccacca
     6421 gctgaataat ctgcgaaagc acggtatcgc ggccaatttt ggttgcttca aacaccagcc
     6481 tgccgttttt gttcagggtc gcgccataaa ccggggagcc ggcgctcttt tccaccggca
     6541 gggactcgcc ggtgagcatg gattcatcta cactggaact gccttccagc acgctgccgt
     6601 ccaccgggat tttctcgccc gggcgcacca caatcacatc gccaatcagc acctcgtcag
     6661 ccggaatttc cgtttccatc ccatcgcgga tgacggtggc tttcttgggc tgcagaaaga
     6721 gcagcttgcg gatggcttcg ctggcgccgc ctttggcgcg ggcttccagg aacttgccca
     6781 ggcgcaccag cgtgataatg accgcggaag tttcaaaata gccgtgcccg ggcaccgcgc
     6841 cgagcagcac aaaaatggaa tagaaatacg ccgcggaggt gcccagcgcc accagcacgt
     6901 ccatatttgc ggatttgttg cgcagggatt tgtaaccgtt cttgtaatag ctggcgccga
     6961 cataaaactg caccggcgtg gccagcgcta agaaaagcca gtccatccag ggggcgtgcg
     7021 ccaccgccat cggcagcagc ccaaagtccc ggcccatcga taggatgaaa agcgggacgg
     7081 taaagatgag cgcgataatc agcaaacgct tctggtgggc ggtctctttc tggcgggcag
     7141 cggcttcggg gtcgtctgtc agcgtgcgtt cttcagccag gcgaaagcca gcccgcagga
     7201 ctgcctggcg gatttcagac tggctgatga tggtgggaac gtaacgcaca accaggcgtt
     7261 cgctggccag gtttgcctgg ctaaaaagca caccttcgac tgtgttcagc gctttttcca
     7321 ggcgcgcgcc gtcggcgggg tctgaaaggc cgatgacgcc caaggaggct tcgcccaatg
     7381 cgacgtcgta gccgctttgg cggatttgtt ccaccagcgc ggggacgtcc gtcttttgcg
     7441 ggtcgtagct gaccgaggcg cgttcggagc ttaagttcac gaccgctgta tccacgcctt
     7501 gcactttgga caagccgcgt tccacagcgg cgacgcagtt cgcgcaggtc atccccagaa
     7561 ttggcagagt cacctgctgc ttatcgggca tttagacctc agccgggtaa ttgatttccg
     7621 caagcgcctg caggatggct tcctccgtcg cggggctgct gaattccact tccacttcgc
     7681 gggtttggac gttgccctcc acgctctcga cgccttcgat ttcgttcagg cggtttttta
     7741 tatgcatcac gcagtgcgcg cagtgaatat tcgggataaa atacgtcttg ctggtcattt
     7801 tgcttccttt ctaatttcag atagaaccag tatcatttta gtcaggtgca agggtatttc
     7861 aaacaagagt ctatacatgg attttttacg cgaggcacaa ggctttattc tcgacatgga
     7921 cggcaccttc tttttaggcg ggcagctgct gccgggcgcg ttggaatttc tggcgctgct
     7981 gaaccgcctt gagctcccct tttccttcct gaccaacaac acttcccgca gtcgggctga
     8041 ttatgtgaaa aaattggtgg ggctcggcgt gggggaggag gacgcgcgcg tgtttaccgc
     8101 cggggatgcc gtcatccgct acttgcgcaa gcattttgac gggcaaggcg tctgcctgat
     8161 tgggacgcgc agcctgcggg aagacttccg ctcggcgggc gtgcggctgg agtaccgcaa
     8221 gccggcggcc gtcgttttag gttacgacac ggagttgacc tacgccaagc tggtgcgatt
     8281 gtgtacgttt gtgcggcagg ggctgcctta cttcgccacg catcccgacg tgaactgccc
     8341 gacgccgaac ggacccatcc cggatatcgg cgcgacgatg gcacttgtgg agaagtccac
     8401 cgggcgcctg ccggataagg tctttgggaa gcccgaccgg aatttgattg agcagctggc
     8461 cggcagcctg aacttgaggc cgcaaagcct ggtgatggtc ggggacaggc tctataccga
     8521 tatcgcgatg ggcacgaccg cgggggtggg gacggtgctg gtgctcagcg gggagacgaa
     8581 gcgggaggat ttgcccggtg cggctttcca gccggatttt gtgtgcgaag acctggccga
     8641 gctggggcgg atgctggaga aggtgcgggg atagaggaag cttgattggt ggaacagcgg
     8701 attgaaaaat ctaagccgcc cgagtacg
//
LOCUS       DAOQAD010000078         8419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_10078_length_8419_cov_5.675036, whole genome shotgun sequence.
ACCESSION   DAOQAD010000078 DAOQAD010000000
VERSION     DAOQAD010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10078_length_8419_cov_5.675036"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKL60_06705"
     CDS             complement(<1..302)
                     /locus_tag="PKL60_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95859.1"
                     /translation="MLDEYNYCNQFPPLVGSLPRVNQLRDKIAFQSKEFGLALFAPAS
                     TINTKKIDSAQAQGGSSNTFRQIAAPPLCKKRSRGFHTTGARNDARFVIASRFAIE"
     gene            326..1471
                     /locus_tag="PKL60_06710"
     CDS             326..1471
                     /locus_tag="PKL60_06710"
                     /inference="COORDINATES: protein motif:HMM:NF025035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HNW95860.1"
                     /translation="MSTRDLDKAKRNKADEFYTQMPDIEAELRHYKEHFRGKVVLCNC
                     DDPYESNFFKYFAMNFNYLGLKKLIATSYSDSPVAYGQLPLFEIQTLKTRPSAEKPAC
                     RIEITEVPDENADGAVDLSDVENLLKSRGNVFSFLEGDGDFRSQECVELLKEADIVVT
                     NPPFSLFREYVAQLVEYDKKFIIIGNKNSITYKEIFQLIKNGKLWLGYRNINQDMWFV
                     VPEHYSYEKIVDGLRVKHIMGCWFTNLDTTKRHESLTLYKRYTPEEYPHYDNYDAINV
                     DKVAEIPCDYDGAMGVPITFLDKYNPDQFVIIGITKTWFGGATKIYPNQTQVNADGKK
                     SVVSKLNDGATLKLNKPPLMQTYYIVDNEVFIQLYARIVIKLKDGKA"
     gene            1468..2550
                     /locus_tag="PKL60_06715"
     CDS             1468..2550
                     /locus_tag="PKL60_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNW95861.1"
                     /translation="MKIELHEISIREVTQSYVDNAEEGVVGYGGRLNIRPKYQREYIY
                     DAAKRDAVLETIRKGFPLNVMYWMRNADGSFEMLDGQQRTISFCQYVNNDFSIENRAF
                     HNLTPPEKEQILNYALMIYICEGDEKERLDWFRVINIAGLKLTEQEMRNAVYTGPWLT
                     HAKSIFSKSGCAAYGLANRYVNGSPIRQEILETALDWISKGNIEAYMGAHQHDPNANE
                     LWTYFRNVIDWVELTFPVYRREMKGLDWGGLYDAYKDALLDTGKLEQEIQSLMMDDDV
                     TNKRGIYAYVLSRNQKTLSIRSFSESQKRAAYERQNGICRSCGQRFEFEEMEGDHITP
                     WHLGGKTLPENCQMLCKDCNRRKAGI"
     gene            2553..3536
                     /locus_tag="PKL60_06720"
     CDS             2553..3536
                     /locus_tag="PKL60_06720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNW95862.1"
                     /translation="MLEKPNKKRLFLFLALAFGLAWLVALVIYLQGGLAQSPVLIPEL
                     NLTLAAALLAGGVMMAPALANLLTRLLTREGKADLWLKPARFANPWRNWLLAWALPAV
                     LTVLGAALFFLLFPSNFDRGLESLRAALSAAAPDQNVSAWTYLLLNTLQALLLAPILN
                     ALFCFGEEFGWRAYLLPKLLPLGERKAALITGVIWGLWHAPVIAMGHNYGLDYPGRPW
                     LGMLAMVLFCAAVGTLLGWLTLKEGSVWPAVIAHGALNGIAGLGLVAVKGAPSVLLGP
                     APTGILGGLFFTLFALGLLLKPGALEPGKEKWHPEPIILPQEPAPKPPEKE"
     gene            complement(3710..4606)
                     /locus_tag="PKL60_06725"
     CDS             complement(3710..4606)
                     /locus_tag="PKL60_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNW95863.1"
                     /translation="MESRVFKQFFQKIEFTPRALRDYLLILLGALVQALSLRLFLVPA
                     SLVSGGLSGAGQLLYYTLGWPIGLVVLLGNLPLFFLGWRYLGGPRFASRTILAIVSFS
                     VFTDLLIYLTGNSALTDDPVLNTVFGGLLLGLGLGIVYLGKGTSGGTDILGRILNRRL
                     GISISMAYMITDSLVVLLAGFVFGWEKALYGLMMIYLSGVAADLASEGANITKAGFII
                     TLKPDAVISAIHAELERGVTMIPVVGGYTRQERSMLYCVVTAAEVMRLKTIVHEADPD
                     AFMVIGQVSEALGEGFKPLLES"
     gene            4974..5678
                     /gene="lexA"
                     /locus_tag="PKL60_06730"
     CDS             4974..5678
                     /gene="lexA"
                     /locus_tag="PKL60_06730"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNW95864.1"
                     /translation="MARRKSGLSPRHKKILAFIEEYQQDKGYPPSIREIGDHTQISST
                     SVVKYYLSQLEEMGYIARQSNVSRGINLIKNATGSLLQNARNTAQALEQLVTIPLVGN
                     IVAGEPMPVPPSDFSYYDPDSGIDIARSLLPAADPLDQLYALRVHGDSMIDAMVNDGD
                     IVVMKKVDQARNGEMVAIWLADRDETTLKYFYQENGRVRLQPANPSMQPIIIDDPSIV
                     EVQGKVVLVIRQLANA"
     gene            complement(5863..6243)
                     /gene="rplL"
                     /locus_tag="PKL60_06735"
     CDS             complement(5863..6243)
                     /gene="rplL"
                     /locus_tag="PKL60_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNW95865.1"
                     /translation="MAELAKLVEELSQLSVLEASELVKLLEEKWGVSAAAPVAAAAAG
                     PAAAAVEEEVEEKTEFTVVIKSAGAKKIEVIKVIRQLTSLGLVEAKTLAETPDAKVLE
                     AVSKEVANDAKAKLEAAGAEVVLL"
     gene            complement(6312..6842)
                     /gene="rplJ"
                     /locus_tag="PKL60_06740"
     CDS             complement(6312..6842)
                     /gene="rplJ"
                     /locus_tag="PKL60_06740"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNW95866.1"
                     /translation="MAFSKQQKEKIVEDYRTWLDKSKAVFVLSYSKMRMPEVNTARAK
                     LREADGQLHVVKNRLFKRVLDERGYQYDAKFWEGNNVVVFAFEDAPSVAKALAEVTKG
                     NEVLAVRGGYLDKAALSVKQVTSLADLPTLPVMRAMLLGTLLAPAGKLVRTIAEPARG
                     LAAVIKANSEKQPTAA"
     misc_feature    complement(6861..7010)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(7051..7773)
                     /gene="rplA"
                     /locus_tag="PKL60_06745"
     CDS             complement(7051..7773)
                     /gene="rplA"
                     /locus_tag="PKL60_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNW95867.1"
                     /translation="MPKHGKKFENALKNIDLEKTYEPKEALKLVKENSFARFDETVEI
                     HLRTGLDPRQADQQIRDVVVLPNGLGKTIRVLVFAQGEAANAARAAGADFVAETDEEI
                     NRVAQGYTDFDVAVGTMDMMGKVGRLGRVLGPRNLMPNPKAGTVVQPADIAGAIKNAK
                     AGRVEFRLDKTANIHVPLGKVSFSVEALEENMTALMSAVRKARPSGAKGLFVRKVTLS
                     STMGPGVKVDPNTAMAMESAKL"
     gene            complement(7830..8258)
                     /gene="rplK"
                     /locus_tag="PKL60_06750"
     CDS             complement(7830..8258)
                     /gene="rplK"
                     /locus_tag="PKL60_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNW95868.1"
                     /translation="MAKKVKAVIRLQITAGKANPAPPIGPALAPHGVNIMAFCKEYNA
                     RTSNRTGEIVPAEITVFMDGSFKFELKSSPAPVLLKKAAGIAKGSATPHTAKVGSVTR
                     AQLREIAEAKFKDMNAVDIEGAMRQLEGTARNMGITVVEK"
BASE COUNT         1867 a         2404 c         2368 g         1780 t
ORIGIN      
        1 aattcgatgg caaaacggct cgcaatgacg aatcgggcgt cgttgcgagc ccccgttgta
       61 tggaatccac gagacctttt tttacacaaa gggggcgcgg caatctgcct gaaagtattg
      121 gaactgccgc cttgagcctg cgcagagtct atctttttcg tatttatggt agaggccggc
      181 gcgaacagag caagcccaaa ttccttactc tggaaagcaa tcttatccct caactgattt
      241 acccgcggca ggctgcccac aagcggcggg aattgattac aatagttgta ttcatcaagc
      301 atgcggggca ggagcgcggg gcaggatgtc gacaagagac ctggataaag ccaagagaaa
      361 taaagcggac gagttttaca cgcagatgcc ggatatcgag gcggaactgc gccattacaa
      421 ggagcacttc cgcggcaagg tggtcttatg caattgcgac gacccctacg agagcaattt
      481 tttcaagtac ttcgcgatga acttcaacta cctggggctc aaaaagctca tcgccaccag
      541 ctattccgat tcgccggtgg catacgggca gctgccgctc ttcgaaatcc agacactcaa
      601 aacccgcccg agcgcggaaa aacctgcctg ccggattgaa atcacggaag tgccggatga
      661 gaacgcggac ggcgccgtgg acctgagcga cgtggaaaac ctgctgaaga gccgcgggaa
      721 cgttttctcg ttcctggagg gcgacggcga cttccgctcc caggagtgcg tggagctgct
      781 gaaggaagcc gatatcgtcg tcaccaaccc gccgttctcg ctcttccgcg agtacgtggc
      841 gcagttggtg gaatatgaca agaagttcat tatcattgga aataaaaact caataaccta
      901 caaagagatt ttccaattaa ttaaaaatgg taagttatgg cttgggtata gaaatatcaa
      961 ccaggatatg tggtttgttg ttccagaaca ttattcttat gaaaaaattg ttgatggcct
     1021 ccgagtgaag catattatgg gatgttggtt taccaacttg gatacgacta aacgccacga
     1081 gagcctgacc ctgtataagc gttacacccc cgaggagtac ccgcattatg acaattacga
     1141 tgccatcaac gtggataagg ttgccgaaat tccctgcgat tacgacggcg caatgggcgt
     1201 cccgataacc ttcctggata aatacaatcc tgaccagttt gtgattattg ggattactaa
     1261 aacatggttt ggaggggcaa cgaaaatcta tcccaatcaa acacaagtta atgccgatgg
     1321 caaaaaaagt gtcgtttcca aactgaatga cggtgctacc ttaaaactta ataaaccacc
     1381 tttaatgcaa acctactaca tcgttgataa tgaggttttt attcaacttt atgcaagaat
     1441 cgtcattaaa ctgaaagatg gcaaagcatg aaaatcgaac tgcacgaaat cagcatccgc
     1501 gaggtgaccc agagctacgt ggataacgcc gaagagggcg tggtcggcta cggcggcagg
     1561 ctcaatatcc gccccaaata ccagcgcgag tacatttacg acgccgccaa gcgcgacgcc
     1621 gtcttggaga ccatccgcaa aggcttcccc ttgaacgtga tgtattggat gcgcaacgcc
     1681 gacggcagct tcgaaatgct ggacgggcag cagcgcacca tcagcttctg ccaatacgtc
     1741 aacaacgact tctctatcga aaaccgggcc ttccacaacc tgaccccgcc ggagaaggaa
     1801 caaatcctca actacgcgct gatgatttac atctgcgagg gggatgagaa agaacggctg
     1861 gactggttcc gcgtcatcaa catcgccggg ctgaagctga ccgagcagga aatgcgcaac
     1921 gccgtctaca ccggcccctg gctcacgcac gccaaatcca tcttcagcaa gagcggctgc
     1981 gcggcatacg ggctggcgaa ccgatacgtg aacggctcgc ctatccgcca ggaaattctg
     2041 gagaccgcgc tggattggat ttccaagggg aatatcgaag cctatatggg cgcgcaccag
     2101 cacgacccca acgccaatga attgtggacc tacttccgca atgtgattga ctgggtggag
     2161 ctcactttcc cggtctaccg cagggaaatg aaaggcttgg attggggcgg tctgtatgac
     2221 gcctataagg atgcgctcct tgacacgggc aaattggagc aggaaattca aagtttgatg
     2281 atggatgatg atgttaccaa caagcgcggc atctatgcct atgtgttgag ccgcaatcag
     2341 aagacattga gtatccgctc tttttccgaa agccaaaagc gggcagctta tgaaaggcaa
     2401 aacgggattt gccgcagctg cggtcagcgc tttgaattcg aagagatgga aggggaccat
     2461 atcaccccct ggcacctggg cggcaagacc ctgccggaga actgccagat gctgtgtaag
     2521 gactgcaacc gcaggaaagc tggtatctga ctttgctcga gaaaccgaac aaaaaacgcc
     2581 tgttcctgtt cctcgccctg gcgttcgggc ttgcctggct ggttgcgctg gtcatctatc
     2641 tgcagggcgg cctggcgcaa agccccgtgc tcatccccga actgaacctg accttggcag
     2701 ccgcgctcct ggccggcggc gtgatgatgg cgcccgctct ggcgaacctg ctcacgcgcc
     2761 tgctcacgcg cgaaggcaag gctgacctgt ggctcaagcc cgcccgcttc gcgaacccct
     2821 ggcgcaactg gctgctggcc tgggcgctgc cggctgtcct caccgtcctg ggcgccgcgc
     2881 tgttcttcct gcttttccct tccaacttcg accgcgggct ggaatctttg cgggctgcgc
     2941 tttccgctgc cgcgccggac cagaacgtct cagcctggac ttatctgctg ctgaacaccc
     3001 tccaggcgct gctgctcgcg cctatcctga acgcgctctt ttgcttcggg gaagagttcg
     3061 ggtggcgcgc ttacctgctg cccaaactgc ttccattggg cgagcgcaag gcggcgctga
     3121 tcaccggcgt gatctggggt ctgtggcacg cgccggtcat cgcgatgggg cataattacg
     3181 gcctggacta cccgggccgc ccctggctgg gtatgctggc gatggtcctc ttttgcgccg
     3241 cggtcggcac gctgttgggc tggctgacgc ttaaggaagg cagcgtgtgg ccggctgtca
     3301 tcgcgcatgg ggccctcaac ggcattgccg ggctgggttt ggtcgcggtc aaaggcgcgc
     3361 cttccgtgct gctggggccg gcgcccaccg gaatccttgg cgggctgttt tttacgctct
     3421 tcgcgcttgg gctgctgctc aagcctgggg ctctcgagcc gggcaaagaa aaatggcatc
     3481 cggagcccat catcctgccg caagaacccg cgccaaagcc gccagagaag gaatagacga
     3541 aaaagcgcca gcctgaacag gcgggcgccg gcagatattg ttggtggatg ccctgcggcg
     3601 tgaatgcggg cgcggcgggt tttctctgtt tgccctcatg gaataaaccg ggaaaggctg
     3661 tccaatccct tttggagaac ccctgccgtt tctcagggaa agagcgggtt cagctttcca
     3721 ggaggggctt aaacccttcg cccagcgcct cgctcacctg cccgatgacc ataaacgcgt
     3781 cggggtccgc ctcgtgaacg atggttttca ggcgcatcac ctccgcggcg gtcaccacgc
     3841 aatagagcat ggagcgttcc tggcgggtat aaccgccgac caccggaatc atcgtgaccc
     3901 cgcgctccag ctccgcgtga atggcagaga tgaccgcgtc gggcttgagc gtgatgatga
     3961 agccggcttt ggtgatgttg gcgccttccg aagccaggtc cgcggccacg ccggagaggt
     4021 aaatcatcat caagccgtag agcgcttttt cccagccgaa gacaaagccc gccagcagaa
     4081 ccaccagcga gtcggtaatc atatacgcca tggagatgga gatgcccagg cgccggttca
     4141 gaatcctgcc caggatgtcc gtcccgccgc tggtgccctt tccgaggtag acaatgccca
     4201 gcccgaggcc gagcagcagg ccgccaaaga ccgtgttgag caccgggtcg tcggtcaggg
     4261 cggaattacc ggtcaggtag atgagcaggt ccgtgaagac ggagaagctg acgatggcca
     4321 ggatggtgcg ggaggcaaag cgcgggccgc ccaggtagcg ccaaccgagg aaaaagagcg
     4381 gcaggttgcc cagcagcacc accagaccaa tcggccagcc gagcgtgtaa tacaacagct
     4441 gaccggcgcc gctgaggccg ccgctgacca ggctggccgg caccagaaac aggcgcaggc
     4501 tgagcgcctg gaccaaggcg ccgagcagga tgagcagata atcgcgcagg gcgcgcgggg
     4561 tgaattcgat tttttgaaag aactgcttga acacgcggct ttccaccttt cctgaaaatc
     4621 gggttgacca aggcaatatt atacgccaaa gccgtttgcg tttccggcgg gttttaaggg
     4681 ctctcttgat tcgacgaaaa ccggaccatc agcaggcatc gggcgcaggg gcgaagcatc
     4741 ttcggcaaat cctgcgatgg aggggaatta tcctctcagg atgcttcgcc cttatgataa
     4801 gctgaagtca cccaaaggcg gccttcatca gccaaattgc ggtgataatc accggcaatt
     4861 gtcgggttgg caggcaccaa ctgcgctcgt ccaaaccctt gacagcgaac atttgttctg
     4921 ttattatcat tctcagaccg ctcccccaac aagagcggag aaagcgagac gaaatggcgc
     4981 gacgaaaaag cggtttaagc ccaagacata aaaaaatcct cgcttttatc gaggaatacc
     5041 agcaggataa aggctacccc ccctctatcc gggagattgg cgatcacacc cagatcagct
     5101 ccacttcagt ggtcaagtac tacctgagcc aattggagga gatgggctac atcgcccgcc
     5161 aaagcaacgt ctcacgcggc attaacctca tcaaaaacgc caccggcagc ctgctgcaaa
     5221 acgccagaaa taccgcgcag gcgctcgagc agctcgtcac catcccgctg gtcggcaaca
     5281 ttgtggctgg cgaaccaatg cccgtgcccc cttccgattt ctcctattac gacccggact
     5341 ccggcatcga catcgcgcgc agcctgctgc ccgctgccga cccgctggac cagctctacg
     5401 cgctgcgcgt gcacggggac tcgatgatcg acgccatggt caacgacggc gacattgtcg
     5461 tgatgaaaaa ggtcgatcag gcccgcaacg gcgagatggt tgccatctgg ctggcggacc
     5521 gcgacgagac caccctcaag tatttctacc aggaaaacgg gcgcgtccgg ctgcagccag
     5581 ccaacccttc catgcagccc atcatcatcg acgaccccag catcgtggaa gtgcagggca
     5641 aagtggtgct ggtcatccgc cagcttgcaa acgcctgaac ctgaaagcgc ttaacaaaaa
     5701 aggctgtcca aagagaaagg gtttttcttt ttggacagcc caagtgagag gtcccgcctg
     5761 atttcggcgc cgtttcgaga gcgcaaataa acaggcgcgg agtgggaaaa ccaaaggggc
     5821 tgcccgactt tggggacagc tcctttgttg tctgcgcggg aattagagca ggacgacttc
     5881 ggcgccagcc gcttcgagct tggccttggc gtcgttcgca acttccttgc tgacggcttc
     5941 gagcactttg gcatcggggg tctcggccag ggttttggct tcgaccaggc ccaggctggt
     6001 gagctggcgg atgaccttga taacttcgat cttctttgcg ccggcgctct tgatcacaac
     6061 agtgaattca gtcttctctt cgacttcttc ttcaacagca gcggcggcag ggccggcggc
     6121 ggcagcggcc actggcgcgg cggcggaaac gccccatttt tcttccaaca acttgaccag
     6181 ttcagaggct tccaacacgg aaagttggct cagttcctct acgagtttag ctaattcagc
     6241 catttcaata tactcctatc attaagttaa ttcttctaat cgatatcccg tcagggtcga
     6301 atgttcaaac gtcaggcagc cgtgggctgc ttttccgaat tggctttgat taccgcagcg
     6361 agaccgcggg cgggctcggc gatagtgcgg acgagtttgc ctgcaggcgc aagcagagta
     6421 cccagcagca tcgcgcgcat cacaggcagg gtcggcaggt ctgccaacga agtaacctgt
     6481 ttgacagaga gggcagcctt atccaggtag ccgccgcgca cagccagcac ctcattgcct
     6541 ttggtaactt cggcaagtgc cttggcgacg gagggagcat cttcgaatgc gaagacgacc
     6601 acgttgttgc cttcccaaaa cttcgcatca tactgatacc cgcgttcatc cagtacgcgt
     6661 ttgaacaaac ggttcttgac tacatgcagt tgaccatccg cctcgcgcag cttcgcgcgg
     6721 gcagtgttca cctcgggcat acgcattttg gaataggaaa gcacgaaaac ggccttgctt
     6781 ttatccaacc aggtacggta atcctctaca atcttttctt tttgctgttt agaaaacgcc
     6841 aatgttgttt cacctccttt cggtgaaaat aaaaatctct ttccgttttt cccgcggaaa
     6901 gagattcaaa tactctcgtg atgtacgaac gttcagtatc caatctccac ctcggcggga
     6961 aattaagccg taacggcacc agctttcttc agtgaagcaa actattcagt tgttttaccc
     7021 ggtcagtata ccacaacccg gggaaagctg ctaaagcttt gcgctctcca tcgccatggc
     7081 ggtgttcggg tccaccttca cgccggggcc catggtcgaa gacagggtaa ccttacgaac
     7141 gaacaaacct ttcgcgccgg atgggcgcgc tttgcgcact gcgctcatca gggcggtcat
     7201 gttttcttcc agagcttcca cgctgaaaga aaccttgccg agcgggacgt ggatattcgc
     7261 ggtcttatcc aggcggaatt ccacgcggcc ggccttggcg ttcttgatag cgccggcgat
     7321 atcagcgggc tgcacaaccg tgccagcttt cgggttcggc atcaggttgc gaggaccgag
     7381 cacgcggcca agacggccaa ctttgcccat catatccatc gtacccacag ccacatcgaa
     7441 gtcggtgtaa ccctgggcga cgcggttgat ttcctcatcg gtttcggcga cgaaatccgc
     7501 tccggctgcg cgggctgcgt tggcagcttc accctgggcg aagaccaaaa cccggatggt
     7561 tttcccgaga ccgttcggca gaacgactac gtcgcggatc tgctgatcgg cctggcgggg
     7621 gtccagaccc gtgcgcaggt ggatttccac cgtctcatca aacctggcaa agctgttttc
     7681 cttgaccagc ttcagggctt ccttcggctc gtaggtcttt tccaggtcga tattctttaa
     7741 tgcgttctca aattttttgc cgtgtttagg catgcttact cctccgtggt gctggcgggt
     7801 tcgttggaac cctcccacgt tcactctggt tatttttcga cgacagtgat gcccatattg
     7861 cgggcggtgc cttcaagctg ccgcatggcg ccttcgatat cgacggcgtt catgtccttg
     7921 aacttggctt cggcaatttc gcgcagctgc gcgcgggtga cgctgccgac cttcgcggtg
     7981 tgcggggtgg cggaaccctt ggcgatgccg gcggctttct tgagcagcac gggggctggt
     8041 gaggacttca gctcaaactt gaacgaaccg tccatgaata cagtgatttc cgccggtaca
     8101 atttcgccgg tgcggttgga cgtgcgtgcg ttgtattcct tgcagaacgc catgatattg
     8161 acgccgtgcg gggccaacgc ggggccgata ggcggagcgg ggttagcctt tcctgccgtg
     8221 atctggagcc tgattactgc ttttaccttt tttgccacaa caattctcct ctgtggtgct
     8281 gacgggccgg cacgcggtcc ctcccacgaa attccggacg gagtccggtt tatgcctttt
     8341 ctacctgcag gaaatccaat tccacagggg tttcgcgccc gaagaagttc accatgacgc
     8401 gaatcttcga gcgttccat
//
LOCUS       DAOQAD010000079         8285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_10300_length_8285_cov_5.063183, whole genome shotgun sequence.
ACCESSION   DAOQAD010000079 DAOQAD010000000
VERSION     DAOQAD010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10300_length_8285_cov_5.063183"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PKL60_06755"
     CDS             complement(<1..563)
                     /locus_tag="PKL60_06755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW95869.1"
                     /translation="MTPAASYDVIIAGAGSIGTPGAYYLAQAGLRVLVLEALPSVGQA
                     SNKHAIGGVRATHSDASKIYLCAESIKILSSWQETHGDDIEWRTGGYCFVAYEQEVKN
                     TLQDLLACQKSYGLKIDWLDAEGIRSIVPDIQPDGLLGGTFSPEDGSASPMKTNFAFY
                     THALAAGAEFHFNEPVIAVGTAQGCIQS"
     gene            complement(560..2677)
                     /locus_tag="PKL60_06760"
     CDS             complement(560..2677)
                     /locus_tag="PKL60_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNW95870.1"
                     /translation="MTDYRIKTHPILPIKPVPYLSFYWNNQECFAKTDEMISSALFAN
                     GIHTFGHHPKDNSPQGLFCANGQCSQCMVIADGLPVKACMTAVKPGMRVFPTEGLPVL
                     PDIPLEPIHTAPTLRISVEALIIGGGPAGLSAAQELGKAGVSVLLVDDKDKLGGKLVL
                     QTHRFFGSSEAVFAGTRGIDIAKKLEAQARAYPDVEIWENSTALAIFEDHSVGVLRAG
                     SQYVLVQPRVLLVAAGARERSLAFPGNTLPGVYGAGAFQTLVNRDLVRPCENLFIVGG
                     GNVGLIAGYHAIQAGIHVAGLVEAAPACGGYRVHLDKLVRSGVPVFTSHTVLEAAGEE
                     RVEKIRIAQVDANFHPIPSTAREFACDTILIAVGLDPVNEFIRKAEEVGLPVFSAGDA
                     EEIAEASAAIFSGRIAGAHIADWLGKSLAPIPDEWVRLEGILKSKPGALREEDLPVEH
                     AGVFPVLHCNQEIPCDPCTTICPKHLIRIDAQDIRKLPQFLRGEAEECIGCLRCVSIC
                     PGLAITLVDFRKRAGTALVSLPYEQEAAALRPGSLIMVTDTSGTALTSLPVHSIRKLA
                     GFSGTHIISVEAPADLAPRIAGLRLIPTSEALEGFHQSDDFPAETADEAYVCRCERVT
                     AGEIRQLIRSGIRDINQIKAVLRVSMGACGGKTCLNMVKRMYQQEGIPLSEVSEPPIR
                     PVFVEMPVSLLANVSRNSDGGAQ"
     gene            complement(2736..3716)
                     /locus_tag="PKL60_06765"
     CDS             complement(2736..3716)
                     /locus_tag="PKL60_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNW95871.1"
                     /translation="MESTETTVTQALNQYLATVRLSRSANTARTYTNAMNLFSQVLTE
                     HKLDLETMPVSALSEEPVIWVIRALKDMAPSTEQCYLTAVVGFYEFLAGENLAAINLP
                     RIRLLIKQRARKPGIRLPQFPKSSIEKVLEYAAGLNLLETKEPNEHLINLRDRAFLLT
                     LADTGLRVHEACNLRRGDIDWDEYRAVIIGKGNREAVLRFSRRSLLAIKDYLRLRAEL
                     DGGSGKALAALPVFARHDKGSGKKVKPISTTTGREIVNQRVGEAIGPEAVGSITPHSF
                     RHYFVTTVLIASGNLKLAQELARHQNIAVTQRYSHLSDAELDKKYLEIFG"
     gene            complement(4321..4734)
                     /locus_tag="PKL60_06770"
     CDS             complement(4321..4734)
                     /locus_tag="PKL60_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95872.1"
                     /translation="MTTIKGSESGATARNRISKSIVLAIRLLMRQKSPDASSRDLAAY
                     VVLALEKISESVETSAIAWEKRDYWLKADRFRQEWAWCEHSAAGLAKALYADDWAGAA
                     QQLADIGQRFGAIEVSENNRLGEPWKGAWETFSRR"
     gene            4871..5437
                     /locus_tag="PKL60_06775"
     CDS             4871..5437
                     /locus_tag="PKL60_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95873.1"
                     /translation="MRTDAAILPFSLPLPPQLLLVIGSHAMSEKMLELVAALALKQRV
                     TLLDCGNRSNMYAVARALRPFTSDPVGVMNRIRLSRAFTCYQVLAMLEAVSAAPPSEP
                     LVILDLLATFLDEDVKLKDCQRLLARSIACLKRLSQSAPVLVSLRPIPPISAARECLM
                     EELRASANAAWEEPVPLPADFGLQPALF"
     gene            5448..5858
                     /locus_tag="PKL60_06780"
     CDS             5448..5858
                     /locus_tag="PKL60_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95874.1"
                     /translation="MGRTIASITQTWQQEEAALKRFQRALRKEDQLLLDELLVLSRLH
                     LAEASYASNLYPLDMYLIAILLELYKQLKALQKELTPEGADPGIKLDSGKEARFLTLL
                     NETLAILAPEAPPAAELPEEASEPRAYTAFEEAP"
     gene            5855..8083
                     /locus_tag="PKL60_06785"
     CDS             5855..8083
                     /locus_tag="PKL60_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNW95875.1"
                     /translation="MNSPNFRGWLLDLYLNQRDGLSLWFISATDDRRVCFTQAFPVAF
                     YAAGAREELRRLWKRLRKESCVCALERQLKQDVFAANPVEVLRIDVDTPAHQAELFRQ
                     LEEEFQELTWYDSDVTISTRYISRHGAFPLALCELSADAQNRIQSIQVLNSRWDLMPE
                     LPPLRVMELTPDADPAHGPARALTLRTDRFSQTLPLSPEGGYLSVLNAALLDYDPDIL
                     VTDWGDSWLLPFLLKQMEEKRVPLALNRDASRPVRWQKEITYFSYGHIIYRPEEAHLF
                     GRCHIDRKSAMMWSDYSLAGTLEMARVTTLPIEKAARVSPGQGISAMQVITALQKDIL
                     VPYQKRQVEEFKSGMQLIQSDRGGMIYQPKLGLHSDVGEVDFVSMYPAVIIRGNISPE
                     VPLPDVLTPASEELGVVPLTLKPIYEKRVALKTKTRSYPPDHPMAKTLKARSSALKWL
                     LVVCFGFLGYKNARYGRIEAHEAVTKGGREVLLRAKEVAEEEGFEVLQMYVDALWLKK
                     KGCSKPEHFEEVMRKIVDRTGLAIALDGVLRWVAFLPSKTDARIPIANRYFGVFQSGE
                     IKVRGLEARRRDTPRWIAQVQTEMLTCLGQAKNRAELPEALRSAFRLYQDALEALKAG
                     RVPLDLLVVNGKVSYALDAYKSATAAVRAARQLEAAGLQIRPGQRVRFLFMQGEPDVR
                     AWELGGKLDPQALNISRYMELLAKAGGSVLLPFGIDPDVLMEWTLKGGVQLPLDAFKN
                     AL"
BASE COUNT         1740 a         2424 c         2417 g         1704 t
ORIGIN      
        1 gattggatgc agccttgcgc tgtcccaacc gcgatgaccg gctcattaaa atgaaattcg
       61 gcgccggcag ccagggcgtg ggtatagaat gcgaagttgg ttttcatcgg cgaggcagaa
      121 ccatcctccg gggagaacgt gccgcccaaa agcccgtccg gctggatatc cggaacaatg
      181 cttcgaatcc cttccgcgtc aagccaatca atcttgagac cataactttt ctgacaggcc
      241 agcaaatcct gcagggtgtt cttcacttcc tgttcatagg cgacaaaaca atagccgccg
      301 gtgcgccatt cgatatcatc cccgtgagtc tcttgccagg aagatagaat ttttatgctc
      361 tctgcgcata ggtatatttt tgaggcgtca gaatgggtgg cgcgcacacc gccaatagcg
      421 tgcttattcg acgcctgtcc aacgctgggc agcgcttcta acaccaggac gcgaagaccc
      481 gcctgtgcca ggtaataggc gccaggggtg ccgatggaac cggcgcctgc tatgatgaca
      541 tcgtaagagg ctgcgggggt cactgggcac cgccgtcaga atttcgggaa acattggcaa
      601 gcagcgagac aggcatttcc acgaaaacgg ggcgtatcgg tggctcgctg acctcggaga
      661 gggggatgcc ttcctgctgg tacatgcgtt tgaccatgtt caggcaggtt ttcccgccgc
      721 aagcgcccat actaacacgc agcacggctt tgatctggtt gatatcgcgt atcccggagc
      781 ggataagctg gcggatttcc cccgcagtta ccctttcaca gcggcataca taggcctcgt
      841 ccgccgtctc tgccgggaag tcatccgact ggtgaaatcc ctccagcgct tccgaggtcg
      901 ggatgaggcg taagcccgcg atgcgagggg ccaagtccgc gggagcttcc acgctgatga
      961 tgtgcgtacc ggaaaaacca gccagcttgc gaatagaatg cacgggcagg gatgtcagcg
     1021 cagtcccgct ggtatccgtg accataatca gactgccagg tcggagcgcc gccgcttctt
     1081 gctcgtaggg taaggacacc agagcggtgc cagcacgctt gcggaaatct accagcgtga
     1141 ttgccaggcc cgggcaaatc gaaacacagc gcaaacagcc aatgcattct tccgcttcac
     1201 cgcgtaaaaa ctgcggcagt ttccggatgt cctgcgcgtc aattcggatc agatgtttgg
     1261 gacagatggt tgtgcacggg tcgcagggga tttcctggtt gcagtgcagc accgggaata
     1321 cgcccgcatg ctcgactggc aggtcttctt cgcgcagagc gcccggcttg gattttagga
     1381 tgccttccag ccggacccac tcatccggga tcggcgcgag gcttttcccc agccaatccg
     1441 cgatatgtgc gccggcaatc cgaccggaaa aaatcgcggc ggaggcctca gcgatttcct
     1501 cggcgtcgcc agcagaaaac acaggcaacc caacctcttc cgccttgcgg ataaactcgt
     1561 tcaccgggtc aagcccgacc gcgatcagga tcgtatcgca ggcgaactca cgcgcggtgg
     1621 atggaatcgg gtggaaattc gcgtcgacct gagcgatgcg gattttctcg acgcgttcct
     1681 cgcccgcggc ttccagaaca gtgtgcgagg taaaaacagg cacgcctgaa cgcaccagct
     1741 tatccaaatg gacgcggtaa cccccgcaag caggggctgc ttctaccagc ccagccacgt
     1801 gaatgcccgc ctggatggcg tgataaccgg cgatgagtcc cacgtttccg ccgcccacga
     1861 tgaacagatt ctcgcagggg cggacgaggt cgcgattgac cagtgtctgg aaagcgcccg
     1921 cgccgtagac tcccgggagg gtattgcctg ggaatgccag actgcgttca cgcgcgccgg
     1981 cagcgacgag cagcacccgc ggctgcacta atacgtattg gctgcccgcc cgcaggacgc
     2041 caacactatg gtcttcaaaa attgccaggg cagtgctgtt ctcccagatt tccacgtctg
     2101 gataggcgcg cgcctgggct tccagctttt tggcgatatc aattccgcgc gtgccggcaa
     2161 aaacagcctc ggaggaacca aaaaagcggt gagtttgcaa aacaagcttc ccacctaact
     2221 tgtctttatc atccaccagt aaaactgaaa cgccggcttt gcccagctct tgcgccgcgg
     2281 agagcccggc cgggccgccg ccgatgatta gagcctccac gcttatccgg agtgtcggcg
     2341 cggtgtggat tggttccagc ggtatatccg gcaaaaccgg caagccttca gttggaaaca
     2401 cgcgcatgcc gggttttacc gcggtcatac aggccttcac aggcagcccg tccgcgataa
     2461 ccatgcactg cgagcactgc ccgttagcgc aaaaaaggcc ttgcggggaa ttatctttgg
     2521 gatggtgacc gaaggtatgg attccgttcg cgaaaagcgc ggaggaaatc atttcatcgg
     2581 ttttcgcgaa gcattcctgg ttgttccaat aaaacgaaag gtaaggtact ggcttaatcg
     2641 gtaagatagg gtgagttttt atccgataat cggtcatagt tccaattgcc aaactccata
     2701 tgtgattgtc ggcaactatg ataccactaa gatgcttaac caaagatttc caggtatttt
     2761 ttgtcaagtt ccgcgtcgct caggtggctg tagcgctggg taacggcaat gttttggtgg
     2821 cgggcaagct cctgcgccag tttcagattt ccggacgcaa ttaataccgt ggtcacaaaa
     2881 taatggcgaa aagaatgcgg cgtgatggag cctaccgcct ccggtccaat ggcttccccc
     2941 acgcgctggt tcacaatttc gcgtccggtg gtggtggaaa ttggtttgac cttttttccg
     3001 ctgcccttgt cgtgcctggc aaacacaggc agggctgcca gagccttgcc gctgccgccg
     3061 tccaactcgg ctcgcaggcg caaataatcc ttgattgcca gcaggctgcg ccgggaaaag
     3121 cgcaggaccg cttcgcggtt gcctttcccg atgatgactg cccggtattc atcccagtca
     3181 atatccccgc ggcgcagatt gcaggcttca tgcacgcgca ggccggtatc cgccaaagtg
     3241 agtaaaaagg cccggtcgcg caggttaatc agatgctcat ttggctcttt ggtttcaagc
     3301 aggttcaagc cggccgcgta ttccagcacc ttttctatcg atgatttggg gaattggggc
     3361 agacggatgc cgggtttgcg ggcgcgctgc ttgatgagca ggcgaatgcg cggcaaattg
     3421 atggcagcca ggttttcgcc tgccaggaac tcgtaaaaac ccacaacggc cgtcaggtag
     3481 cactgctcgg tagagggcgc catgtctttg agcgcccgga tgacccagat gaccggctct
     3541 tcgctgagcg cgctgactgg catggtttcc aggtctagtt tatgctccgt caggacctgg
     3601 ctgaagaggt tcatcgcgtt ggtgtaggtg cgggcggtgt tcgcgctgcg ggagaggcgg
     3661 actgtggcga ggtattggtt tagggcctga gtgactgttg tttctgtgga ttccataatg
     3721 gcattatacc gctttccata tattggttgc gataaatgca taaagaatac atattatccc
     3781 attaattatt cctatattgc gtatagtata gtggtagttt tttaccaggt catcccgatc
     3841 tttgcaaagc acttacgctc gaatttccag cggaacacat gccacattcg gttcggggcc
     3901 tgcacggcag cctgtttgct tgccgcgagg cgacagggag atagcaaata tccaacagga
     3961 ccgcttacgg caatcctcct caggagagac gcggttccga acgatgcaaa gcaaaaaagg
     4021 gtctcagttg ggaaatcgag ttgggcggga gacacaccgg ctttcgcggt caaggacacg
     4081 atactgtaat agcaccgtgc ggttcatttt gagttgcttt cggtacggag tatgtgccgc
     4141 gtttgctttc ccctgaaaca cagatcagcg cggaatatcg accgcaagcg ctaacttggt
     4201 agcctgggtt gagaacgata agcttacgat aatgatactt atcataacca taaaattgaa
     4261 tctcagaaaa atcgctcccc tgcctctgcc ctggaacagg aatttagacg ctctgccggt
     4321 ttaacggcgc gagaacgttt cccaggcgcc tttccacggt tcacccaacc ggttgttctc
     4381 gcttacttct atcgcgccga agcgctgccc gatatcggcc aactgctggg cggcgcctgc
     4441 ccagtcatcc gcgtataacg cctttgccaa cccggccgcg ctgtgctcac accaggccca
     4501 ctcctggcgg aagcggtccg ccttgagcca gtagtcgcgt ttttcccagg caatcgcgct
     4561 cgtttccaca gattcgctga ttttttcaag cgccaggacc acgtacgccg ccaaatcccg
     4621 gctggacgcg tccggcgatt tctgccgcat caacagccgg atagccaaca caatggattt
     4681 actgatgcgg ttgcgcgcgg tcgcgccgct ttcactgcct ttgatcgttg tcattcttcc
     4741 tcgtcacaga taatttcaat ccccaattat acccaccctg ccctacccac gccgccagaa
     4801 aaaggataga attagcacag ctgttctgtt aatctcttga catagaacaa atattctata
     4861 taatagaggt atgagaacag atgccgcgat actccccttt tcactgcccc tgccgcccca
     4921 gctgctgctg gtgattggca gccacgcgat gtccgaaaaa atgttggaac tggtggccgc
     4981 cctggccctg aagcagcgcg tgaccttgct ggactgcggc aaccgatcca atatgtacgc
     5041 ggttgcccgg gctctccggc ctttcaccag cgatccggtc ggcgtgatga acagaatccg
     5101 cctctcgcgc gcctttacct gctaccaggt gctggcgatg ctggaagcgg tttccgccgc
     5161 cccgccgagc gaaccattgg tgattctgga tctgctggca acctttctgg acgaagacgt
     5221 gaagctgaaa gactgccagc gcctgcttgc ccgcagcatt gcctgcctga agcgcctcag
     5281 ccagtccgcg ccggtgctgg tgagcctgcg gcccattccg cccatatccg cggcgcgtga
     5341 atgcctgatg gaagagctgc gcgccagcgc caacgctgcc tgggaagaac ccgtcccact
     5401 gccggccgat tttgggctgc agccggcgct gttctagaaa ggaacccatg ggccgcacaa
     5461 ttgcttccat cacccaaacc tggcagcagg aagaagccgc cctaaaacgc tttcagcgcg
     5521 cgctgcgcaa agaggaccag ctgctgctgg atgaactgct ggtgctctcc cgcctgcacc
     5581 tggccgaagc ctcgtacgcg tccaatctct acccgctgga tatgtatctg attgccatcc
     5641 tgctggagct gtacaagcag ctgaaagcgc tccaaaagga gcttaccccg gaaggcgcgg
     5701 accccggcat aaagctggac agcggcaaag aagcgcgctt tctcacgctg ctcaacgaga
     5761 cgctggcgat tttggcgccg gaagccccgc ccgccgcgga actgccggaa gaagcgtccg
     5821 aaccgcgcgc gtacaccgcg tttgaagagg cgccgtgaac agcccgaatt tccgcggctg
     5881 gctgctcgac ctgtacctga accagcggga cggcctgagc ctgtggttca tctccgcaac
     5941 ggacgaccgg cgggtgtgct tcacacaggc cttcccggtg gcgttttacg ccgcgggagc
     6001 acgcgaggag ctacgccggc tgtggaaaag gctgcgtaag gagagctgcg tgtgcgcgct
     6061 ggagcgccag ctaaagcagg atgtgttcgc cgcgaacccc gtggaagtgc tgcgcattga
     6121 tgtggacacc ccggcgcatc aggccgaatt gttccgccag ttggaggagg agtttcagga
     6181 actgacctgg tacgattcgg acgtaaccat cagcacgcgc tacatcagcc ggcacggcgc
     6241 attcccgctg gcactgtgcg agctcagcgc cgacgcgcaa aaccgcatcc agtccatcca
     6301 ggtgctcaac agccgttggg acttgatgcc ggagctcccg cccctgcgcg tgatggaact
     6361 gacgccggac gcggaccccg cgcacggccc ggcccgcgcg ctcaccctgc gcacggaccg
     6421 cttcagccag acgctgccgc tcagcccgga gggcgggtac ttatccgttc tgaacgccgc
     6481 gctgctggac tacgacccgg atattctcgt taccgactgg ggggacagtt ggctgctgcc
     6541 ttttttgctc aagcagatgg aggagaagag agtgcccctg gcgctgaacc gggacgccag
     6601 ccggccggtg cgctggcaga aagaaatcac ctacttttcc tacgggcaca ttatttaccg
     6661 cccggaagaa gcgcacctgt tcgggcgctg ccatattgac cgcaaatccg ccatgatgtg
     6721 gtcggattac agcctggccg gcacgctgga aatggcgcgt gtgaccaccc tgccaatcga
     6781 gaaagccgcg cgcgtctcgc ccggtcaggg tatttccgcc atgcaggtca tcaccgcgct
     6841 gcaaaaggac atcctggtgc cttaccagaa acgccaggtg gaggaattca agagcggcat
     6901 gcagctcatt caatccgacc gcggcgggat gatttaccag cccaaactgg ggctgcacag
     6961 cgatgtgggc gaagtggatt ttgtttccat gtaccccgct gttattatcc gcggcaacat
     7021 ctcgccggaa gttcccctgc cggatgtgct gacgcccgcc agcgaagaat tgggcgtggt
     7081 cccgctcacg ctcaaaccca tttacgaaaa acgcgtagct ctgaagacaa agacgcgctc
     7141 ctacccgccc gaccatccca tggcgaaaac gctcaaagcg cgctcatccg ccctcaagtg
     7201 gctgctggtg gtttgttttg gctttcttgg ctacaaaaac gcccgctacg gacgcatcga
     7261 agcgcacgaa gcggttacca agggcggtcg cgaggtgctt ctgcgtgcca aagaggtggc
     7321 ggaggaagaa ggttttgaag tgctgcagat gtatgtggac gcgctctggc tcaaaaagaa
     7381 gggctgcagt aagcccgagc actttgagga ggtcatgcgc aagattgtcg accgcaccgg
     7441 gctggcaatc gcgctggacg gtgttttgcg ttgggtcgcg tttctgccgt ccaaaacaga
     7501 tgcgcgcatc ccaatcgcga accgctattt tggcgtgttc cagagtgggg aaatcaaagt
     7561 gcgcggattg gaagcccgcc gacgggatac cccgcgctgg attgcacagg tgcaaactga
     7621 aatgctcacc tgcctggggc aggcaaaaaa ccgtgctgag ctgcccgaag cgctccgctc
     7681 ggcttttcgg ctttaccagg atgcgctgga agcgctgaaa gccggccgcg ttccgctgga
     7741 cttgctggtg gttaatggca aggtcagcta tgccctggac gcctacaaaa gcgcgaccgc
     7801 ggccgtgcgc gcagccaggc aattggaagc cgccggcctg caaatccgac ccgggcagcg
     7861 cgtgcgtttt ctttttatgc agggcgaacc ggacgtgcgc gcctgggaac tgggcggtaa
     7921 gctggacccc caagcgctga acatctccag gtacatggag ctgctggcaa aagccggcgg
     7981 aagtgtgctg ctgccctttg gcatcgaccc ggatgtcttg atggaatgga cgctgaaggg
     8041 cggcgtgcag ctgccgctgg atgccttcaa gaatgcactg taggagttgg aacgctgaaa
     8101 tcagccatcc cgcgccggga gctgcttgag cgcgggagcg ctgcgtcccg ccttgccccc
     8161 agcgtcgctg ttaggagagg aacgtttgca ggagaaaata gctcctgtta tgcggataac
     8221 ccaagcagca gtgccaatgc agaattttgc ggtcctggta ttggagggtt tttaaagcaa
     8281 ggcaa
//
LOCUS       DAOQAD010000080         8212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_10423_length_8212_cov_5.193331, whole genome shotgun sequence.
ACCESSION   DAOQAD010000080 DAOQAD010000000
VERSION     DAOQAD010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10423_length_8212_cov_5.193331"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..1290)
                     /locus_tag="PKL60_06790"
     CDS             complement(289..1290)
                     /locus_tag="PKL60_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95876.1"
                     /translation="MVWIEFLISAAIITFVSIQLAKYGDVIGLRTKLGGAFAGTLLLA
                     TATSLPEVITTISAVRTGIPQLAAGNLFGSNMFNILLLAILDLAHYRRRISRKNAEKH
                     ALSGSLAVLMIVLAIFFIIAQLPLQIKVGNFVVGADAMVMIAAYFLAMSLLRKQSRIS
                     SIPVRPEDIPEDVPPLRTAILWFVSAIVVLVFVTPWMVDASGRIAEITGLGATFIGST
                     LVAVVTSLPELITTISAAKIGADDMAIGNLFGSNMFNMFGLGIADFFFFGGRFFAVID
                     PSFLLIGVIGLLMTVFALIATLARLERRILFLEIDSLILILAYFGGLALLYARGIAP"
     gene            complement(1292..1633)
                     /locus_tag="PKL60_06795"
     CDS             complement(1292..1633)
                     /locus_tag="PKL60_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95877.1"
                     /translation="MFNILFVLDDPDKLPELLEAWEEAGISGVTILESTGMYRLKRRL
                     FPMRYLPVVYDQEESHLTLMAIVDDEQLIQACLRATEKVIGSLEEPNTGIFAAWPLTV
                     VKGIPKKGAKP"
     assembly_gap    2198..2297
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2567..7714
                     /locus_tag="PKL60_06800"
     CDS             2567..7714
                     /locus_tag="PKL60_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNW95878.1"
                     /translation="MKTIRLPLALLLALSLLLSPAASLAQSSPSAEPASPPSALNLAG
                     LNWCVAGEINGWNNSSDPMHDDGTHGDMLALDGVYSLMFTIASAGTYQWKAVECGNWG
                     NAHPAANSWVTTSAANQVVTFMLDTNDYSANAGKPLLPTANIVNVSGESLPASFTAVG
                     DFQGWNNTDPNTLLTDMGKGYYRLVYTIPSAGSYIGKVVLTGSWNGFGADGRSTDAAN
                     ISFTTTTADQQVVFLLDSFTGRLLIEPVEAGIAAWCAAGAFNGWSASADPLYDDGTHG
                     DLLGGDGIFTADVTFPEAGRIEWKVTNCTWDISYPTTGNSWLTTSSAAQVVKLSFDTN
                     DHSADAGLDMLPVVNILQAWDSTNSFTAVGDFQGWNNTNPDTALTALGHDLYRLDALF
                     VTAGNYVFKVVTTGSWDAIGLDNRSVNANVIPFTTNNDADLVPFLLDYRSGRVGAFPP
                     PAGEPPLPGHDNEVWWDYLGHDSRDTLYSVPGGPVPTGTEITLRMRAAQGDLTAAKLR
                     LYNDRTNVQSILPMTVAYSDGTYDWWEYTIPASSDPTVYWYRFIAIDGTDTDYYEDDA
                     ARTGGWGQAFDESQDYSYQLTVYAADFQTPDWIKDAIVYQVFPDRFRDGDPGNNKPAG
                     TFFYDEPGGTVYRSLTDEWNTPICDPRETGDCAGTYSKNFYGGDLAGLISKLDYLQDL
                     GVNTIYLNPIFESPSNHGYDTSDYMSINPMFGEPAEFFALVTALNNRGMHLILDGVFN
                     HVSSDSIYFDRYGNFDEVGACESISSPYRAWFYFTDVPAGTGPCVGSDGTPGGATYES
                     WWGYDSLPKLNSSNPEVRALIWEYESDALTTVAGYYMQYADGWRLDVGGDVDPGTIND
                     PTNDYWEGFRNTVKIVNPDGYIAGEEWGIANSWLLGGEWDAVMNYQFSSAVLSLWRDS
                     TFTDNDHTAGSSAGELAPLSMSQFDERILNLQERYAPEALAAMLNLFGSHDTSRALFM
                     LDHNTYLNDPSLYQDPDYDWSDAINRLKGAVAVQMTMPGAPTIYYGDEVGLVGPMAYA
                     GGKWEDDPYNRQPFPWLDASGTPFYTRLQTQESQDEIYNYYVDLTSERNTHPALRTGD
                     YRTLLVENTLKLYAYGRNLPGEDSAVILINRGSGAQSTDLDVVGYLPIGTTYLDVLSD
                     SVYTVNSEGKLPAVSAPANGAAVLVRTNEQVAPPEPVELSAVTDGTDVTLSWTASDGA
                     SNYLVYRSYLRGGGYELRDITSNTSYTDSEQISGQRVYYIVIARNLPNGLQSENSNEV
                     SALPAYIIDWANLQWPFSISHTLSSTPTENIYGRVYIAGVTSEPGPTPGLRAQVGFGP
                     NESDPRGNSAWTWFEASFNGQAGNNDEFVGSLLPEAVGEYDYVYRYSTVGAESWVYAY
                     DGGLVGDPYTPANAGDLTVNPSSDLTPPSEPVLAVEDWSAAHIALAWTAATDDIAVYA
                     YDLYRSTDGVNFTRLARILDPVLTYDDESVETGETYSYKIQAVDTSFNFGGFSNVVSQ
                     TAEAKLVAVTFEVTVPDYTPGTVYLTRFINADGTIGDWNPAASALTQSSSTLWTGTFD
                     ILDGTQMDFKFTRGSWETVIKGAAHEELPNLTLSVDYGEDGTQLYAYTVLNWRDPIVT
                     DVSPADGSLGVSVDSVIITNWSQPMPADACFTVSGPLGAIAGSCTYDSGYQAITFTPT
                     EYLLPQKTYTVTASGLVDEHGDVQQVAFTSTFKTGGELLIYMPLIFK"
     gene            7979..>8212
                     /locus_tag="PKL60_06805"
     CDS             7979..>8212
                     /locus_tag="PKL60_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95879.1"
                     /translation="MPAQEIFVNRVFVTLSAAKGRSVRQYLTGISLPTAAGSRVRLNL
                     DKLNKKATLRRHFLNEKSHGDAPASDRREWDCLA"
BASE COUNT         1807 a         2506 c         2197 g         1602 t
ORIGIN      
        1 gtgtgttctt gtcattctgc tgccctgaag ggaacgtgcc gaagcgaaga atcacctcag
       61 ctaaataaaa gcccttcagc tgcgctcagg atgacaaccc ttttgtcatt ccgctgccct
      121 gaattgccca tgccgcagcg aagcatctcc tcacattcaa accggggttc ttccccctcg
      181 ctcaggcgga atcgaatcct agacgggcag cggactgctg tttggaagtg atgacaagca
      241 aagatctgcg ggcacagctg ttctttgaaa tgcagggcaa tcccgcgctc acggcgctat
      301 cccccgtgcg tacaacagcg ccaggcctcc gaaatatgcc aggatcagga tgagtgaatc
      361 aatttccagg aagagaatcc gccgctccag acgcgcaagc gtggcgatca gcgcgaaaac
      421 agtcattaac aggccaatca cgccaatcag caggaatgaa ggatcaatca cggcaaagaa
      481 acgcccgcca aaaaagaaaa aatccgctat acccagcccg aacatattaa acatgttgct
      541 gccaaacaga ttgccaatcg ccatatcgtc cgcgcctatt tttgcggccg aaattgttgt
      601 gatcagttcc ggcagggagg tcactactgc cacgagtgtc gaaccgatga aagtcgcgcc
      661 cagaccggtg atttccgcga tgcgcccgct ggcatccacc atccagggcg taacaaacac
      721 cagcacaacg attgcgctta caaaccataa aatcgcagtc cttagcgggg gaacatcctc
      781 cgggatgtct tcaggtctca ccgggatgga ggagatacgg ctctgtttgc gcagcaggga
      841 catagccaga aagtaagccg cgatcatcac cattgcgtcc gctccgacga cgaagtttcc
      901 aacctttatc tgcaagggca gctgtgcgat gatgaagaaa atcgccagca cgatcatcag
      961 caccgccagg cttccggaaa gagcatgttt ttccgcgttt ttgcggctga tgcgtctgcg
     1021 gtagtgcgcc aaatccaaga tcgccagcaa gaggatattg aacatgttac tgccaaacag
     1081 gttgccggct gccagctgcg ggatgcctgt gcgcacagcc gaaattgttg taatcacctc
     1141 cgggagagaa gtagcagtcg ccaaaagcag cgtgcccgcg aaagccccgc ccagtttcgt
     1201 gcgcagcccg atgacgtcgc cgtattttgc gagctgaatg gaaacaaaag tgataatcgc
     1261 ggcgctgatg aggaactcta tccagaccat ttcagggctt tgctcccttc ttggggattc
     1321 ctttcacgac cgtcagcggc caggccgcaa atatgccagt gttgggctct tccaggctgc
     1381 cgataacctt ctcggtagcg cgcaggcagg cctggatcag ctgttcgtca tcgacgattg
     1441 ccatcagcgt cagatggctt tcctcttggt catagactac cggcaggtag cgcatcggga
     1501 acagccggcg cttaaggcgg tacataccgg tgctctccag gatggtcacc ccgctgatac
     1561 cggcttcttc ccaggcttcc agcagctccg gcagcttatc ggggtcatcc agcacaaata
     1621 atatgttgaa cataggcact cctcaggttt taggcggcgg ttttccttca ggttggtaat
     1681 ggtgaagata atcaagatgt tgccggcaga tttgccgtaa tactatgaac cgccacataa
     1741 ctattttacc ccaggcaggc gggtcggaaa atggcgctgt agaatgagga cctcgcaaat
     1801 ttttaccggc ggcaaatgaa gagatggagc gcccagttat tccaccgccc tgaagggcac
     1861 gcgccgcagc gaagaaaata ctgattaaac ggtcgtaggt cagcgcggga gtgaattgtc
     1921 attctgatgc cctgaagggt acgtggcgaa gcgaagaatc tcctcacgaa aaaagccaga
     1981 cccttcgact gcgctcaggc tgataacatt tttatcattc cgctgccctg aagggtgcgt
     2041 ggcgaagcga agaatcttcc cttaacaatc cagacccttc ggctgcgctc aggctgtcta
     2101 tgctggtcat tctgatgccc tgaagggtac gtggcagaac gaaagaatct tctcacggaa
     2161 aaaaccgaga ccttcgactg cgctcaggct gataacannn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnaag aatcttctca cggaaaaaac cgagaccttc gactgcgctc
     2341 aggctgataa catttttgtc attccgctgc cctgaattgc ccatcctgca gcaaagaatc
     2401 tccaaccgca tccaccaaag cctttctgct tcggcctgta cccctaaggg ctcgagacca
     2461 cagacaggaa taaagccctc cccgccatct ggctaaatca tcttaatttt tgttcacata
     2521 tcaactataa tgatgattac tcaccaatat attcagaaag ggagatatga aaacgatacg
     2581 tttgcctctt gctttgcttc tggcgctatc actcttgctc agcccggcag cctcgctggc
     2641 tcagagcagc ccgtccgcag aaccagcctc accgccctca gccctcaacc tggccgggct
     2701 caactggtgc gtcgcaggcg aaataaacgg atggaataat tcctccgatc cgatgcacga
     2761 cgacggcacc cacggcgaca tgcttgccct ggacggcgtc tattccctca tgttcaccat
     2821 cgcctcggct ggcacctacc agtggaaagc ggtggaatgc ggcaattggg gcaacgccca
     2881 cccagccgca aactcctggg tcaccaccag cgctgccaac caggtcgtga ccttcatgct
     2941 ggacacgaac gattacagcg cgaacgcggg caaaccctta ctgcccacag ccaacatcgt
     3001 gaacgtctcc ggcgagagcc tcccggccag cttcaccgct gtgggcgact tccagggctg
     3061 gaataacacc gaccccaaca cgctcctgac ggatatgggc aaaggctatt accgcctggt
     3121 ttacaccatt ccaagcgccg gcagctacat cggtaaagtg gtcctgaccg gcagctggaa
     3181 cggatttggc gcggacgggc gctcgaccga cgccgccaac atcagcttca ccaccaccac
     3241 agccgaccaa caggtcgttt ttctgctcga cagcttcacg gggcggctgc tcattgaacc
     3301 cgtcgaagca ggaatcgccg cctggtgcgc cgccggcgcg tttaatggct ggtccgccag
     3361 cgcggacccc ctttacgatg atggcacgca tggcgacctc ctggggggtg acggcatttt
     3421 cacagccgat gtcaccttcc cggaggctgg tcgtatcgaa tggaaagtga ccaactgcac
     3481 ctgggacatt tcctacccga cgaccggaaa ttcctggctt actaccagct cggcggctca
     3541 ggtggtcaag ctgagcttcg ataccaatga ccacagcgcg gatgcaggtc tggacatgct
     3601 gccggttgtc aacatcctgc aagcctggga ttccaccaac agcttcactg cggtgggcga
     3661 ctttcagggt tggaataaca ccaatccgga tactgccctc acagccttag ggcacgacct
     3721 ttaccgcctg gacgcgctct tcgtcaccgc tggaaattat gttttcaaag ttgtcacaac
     3781 cggctcatgg gatgcaatcg ggctggacaa ccgctcggtg aacgcaaacg ttatcccgtt
     3841 cacgaccaat aacgatgcgg acctcgtgcc cttcctgctg gactaccgca gcgggcgggt
     3901 cggcgctttc ccaccgcctg ccggtgaacc cccgctgccg gggcatgaca acgaagtctg
     3961 gtgggactat ctgggtcacg acagccgcga cacgctttac agcgtccctg gcgggccggt
     4021 acccacgggc acggaaatca ccctgcgcat gcgcgccgct cagggcgacc tgaccgccgc
     4081 caagctgcgc ctctataatg accgcaccaa tgtgcagtcc atcctgccga tgacggtggc
     4141 ctactccgac ggcacctacg actggtggga atacaccatc cccgccagct ccgacccgac
     4201 cgtttactgg taccgcttca tcgccattga cggcacggat accgactatt atgaagatga
     4261 tgctgcccgc acaggcggct ggggccaggc ttttgacgag agccaggact attcttacca
     4321 gcttaccgtc tacgccgcag acttccagac ccccgattgg atcaaggatg cgatcgttta
     4381 ccaggtcttc cccgaccgct tccgcgatgg cgacccaggc aacaacaaac ccgccggcac
     4441 cttcttctac gatgagccag gcggtacggt ctaccgctcg ctgactgatg agtggaacac
     4501 ccccatttgc gacccgcgtg aaacgggcga ctgcgccggt acctacagca agaacttcta
     4561 cggcggcgac ctggccggtt tgatatccaa gctggactac ctgcaggacc tgggcgtcaa
     4621 taccatttac ctcaacccga tttttgagtc gccttccaac cacggctacg acaccagcga
     4681 ttacatgtcc atcaacccga tgtttggcga gccggcggag ttcttcgccc tggtcaccgc
     4741 gctgaataac cgcggcatgc acctgatcct ggacggcgtg ttcaaccacg tctcttccga
     4801 cagcatttac tttgaccgct acggcaactt tgacgaagtc ggcgcgtgtg agagcatcag
     4861 ctccccgtac cgcgcctggt tctacttcac cgacgtcccg gccggcaccg gcccctgcgt
     4921 gggttcagac ggcacccccg gcggggcgac ttacgaatcc tggtggggct acgacagcct
     4981 tcccaagctc aactccagca atcccgaagt gcgcgcgctg atttgggaat acgaaagcga
     5041 cgcgctcacc accgtggccg gctactatat gcagtatgcc gacggttggc gcctggacgt
     5101 gggcggagac gtggatcccg gcacgattaa cgacccaacc aacgactatt gggaaggttt
     5161 ccgcaacaca gtcaagatcg tgaacccgga cggatatatc gccggcgagg aatggggcat
     5221 tgccaacagc tggctgctgg gcggcgagtg ggacgcggtc atgaactacc agttcagttc
     5281 agccgtcctt tcgctttggc gcgacagcac ctttactgat aacgaccaca ccgccggttc
     5341 ttccgccggt gagctggcgc ccctcagcat gagccagttc gacgagcgca tcctgaacct
     5401 gcaggaacgc tacgcccccg aagcgctggc cgccatgctc aatctcttcg gcagccacga
     5461 taccagccgc gcgctcttca tgctcgacca caacacctat ctaaacgacc ccagcctcta
     5521 tcaggatccg gattacgact ggagcgacgc aatcaacagg ctaaagggcg cggtagcggt
     5581 gcagatgacc atgcccggcg cgccgaccat ctattacggc gatgaagtcg gcttggtggg
     5641 acccatggct tacgccggcg gaaaatggga ggacgaccct tacaaccgcc agcctttccc
     5701 gtggttggac gccagcggaa cgccgttcta tacgcgcctg cagacccagg aaagccagga
     5761 cgagatttac aattactacg tcgaccttac cagcgagcgc aacacgcacc ccgcgctgcg
     5821 caccggggat taccgcaccc tcctggtaga aaacacgctg aaactctacg cttatgggcg
     5881 caatttgccg ggcgaggact cggcggttat actcattaac cgcggcagcg gggcccaaag
     5941 cactgacctg gacgtcgtag gctacctgcc gattggcaca acctatctgg acgtgctttc
     6001 cgactccgtc tacaccgtta acagcgaagg gaaactcccc gcggtgagcg cgcctgccaa
     6061 cggcgccgcg gtgctggtgc ggacgaacga acaggttgct ccacccgaac cggtggaact
     6121 gagcgcggtc acggacggca ctgacgtcac cctgagctgg acggcttcgg acggcgcgag
     6181 caactacctg gtgtaccggt cttacctgcg cggcggcggt tacgaactgc gcgatataac
     6241 ctccaatacc agctacacgg attctgaaca gattagcggt cagcgcgtct attacatcgt
     6301 catcgcgcgg aacctcccga atgggctgca gagcgagaat tccaacgagg tcagcgcgct
     6361 tccggcctac atcatcgact gggcgaacct gcagtggccg ttcagcatca gccacaccct
     6421 cagcagcacc ccgaccgaga acatctacgg acgggtctac atcgccggcg tcacttccga
     6481 gcccggcccg acccccgggc tgcgcgccca ggttggtttt ggccctaacg aatccgaccc
     6541 gcgcggaaac tccgcctgga cctggttcga ggcaagcttt aacgggcaag ccgggaataa
     6601 cgacgagttc gtgggttcac tccttccaga agcagttgga gagtacgatt acgtctatcg
     6661 ctattccacg gtcggcgcag aaagctgggt ttacgcttat gatggcggac tggtgggcga
     6721 cccctacacg cccgcgaatg ccggcgacct gaccgtcaat ccctcctcag acctgacccc
     6781 accctctgag cctgtgctgg ctgtggagga ctggagcgcc gcgcacattg ccctggcctg
     6841 gacggcggca acagacgaca tcgctgttta tgcttatgac ctctaccgct ctaccgacgg
     6901 ggtgaacttc accaggcttg ccagaattct ggaccccgtt ctcacttacg atgatgagag
     6961 cgtcgaaacg ggggagacct attcctacaa aatccaggct gtcgatacca gcttcaactt
     7021 cggcggcttt tccaacgtcg tcagccagac tgcggaagcc aagctggtgg ctgtcacctt
     7081 tgaggtgacc gtgccggact acactcccgg caccgtctac ctgacccgct ttatcaatgc
     7141 ggatggaacg attggcgatt ggaacccggc tgccagcgcc ctcactcagt ccagcagcac
     7201 gctttggacc ggcactttcg acatcctgga cggcacgcaa atggatttca aattcacgcg
     7261 cggcagctgg gaaacggtca tcaaaggcgc tgcgcatgaa gaactgccca atctaacgct
     7321 gagcgtggat tacggcgagg acggcacgca gctttatgcg tataccgtcc tgaactggcg
     7381 cgacccgatt gtcacggacg tgagccccgc ggatgggagc ctgggggtta gcgtggacag
     7441 cgttatcatc acaaattgga gccaacccat gcccgcagac gcctgcttta ctgtttcggg
     7501 tccgcttggc gcgattgccg gaagttgcac gtatgacagc ggataccagg caatcacctt
     7561 cacgcccacc gagtatctcc ttccgcagaa gacctacacg gtcaccgcca gcgggttggt
     7621 ggatgagcat ggggacgtcc agcaggtagc gttcacgtcc accttcaaaa ccggcggcga
     7681 attgctgatc tacatgccgt tgattttcaa gtaactatca ggaatacgaa aacgcctggc
     7741 ggttctcacc gccaggcgtt tttatttgac aggccttgtt tttttgtcga attgagcaca
     7801 gcgaaggggc tgaattgtgt tttcccggcc tcaaggacgg ggaaagctga cgaccgcggg
     7861 gctccgagtg tgaagcgaac aatgcctgtc accctgagcg cagcgaaggg gctggcctgt
     7921 acttgctctt gcagggtctc cggagtatag gcatgcattt caggtacgag gtacttccat
     7981 gcccgctcag gagatcttcg tcaacagagt ttttgtcacc ttgagcgcag cgaagggacg
     8041 ttcggttcgg caatatctca cgggcatctc cctgcccacg gccgcaggga gtcgggtccg
     8101 cctcaacctt gacaagttaa acaaaaaagc caccttacgg cggcatttct taaatgaaaa
     8161 gtctcacggg gatgcacctg cctccgaccg tagggagtgg gactgccttg ca
//
LOCUS       DAOQAD010000081         8179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_10496_length_8179_cov_5.197070, whole genome shotgun sequence.
ACCESSION   DAOQAD010000081 DAOQAD010000000
VERSION     DAOQAD010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10496_length_8179_cov_5.197070"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="PKL60_06810"
     CDS             complement(<1..1746)
                     /locus_tag="PKL60_06810"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNW95880.1"
                     /translation="MPPHLQPLTKPELLRQLRAARAAFDITVNAVPPERFEETLPGGE
                     WSTKDTLTHLTFYERQLAERLWEQLERRPHTVVDTDFMTWQEQNPIIRERHKDDPVEK
                     TLQDSHAAFESLWNALSTLPEEELFRPQIWEGVPEPVFIPDVLHNDVTDHYPEHIENI
                     RRLFPGLSERSSAMPPTKTNKPFGTWLSPITPELIGGSARFSDVQWAPGSDRLVWCGS
                     LSGKASLLTQVGLDAPSELSAGFNPSGSIGYGGGEFCAGVGGAVFAEKDGRLYFAPYG
                     PGSPQALTPAFGRCASPKLSPAQDRVLFVHSYEERDVLALVRLDGKSWPRVFASGADF
                     YMQPTWSPDGKAAAWVEWDHPDMPWDGTRLMFAVLDESGGAPAEAHLLDGGPETPIFQ
                     PEFSPDGRYLAYLRNPGEWDQLVLFDLQTGEKRILLDGRSLLPPAWVQGSRVLAWAPE
                     SSALYFLENQQAKVYLRKVDIRSGESQAVDIRAFTNLEQLCVSASGALALIAQSPSLP
                     ARIIILQAGQMRIAARSRADDLHPEDLPQAQEVDWLSSDGARVYGTYYAPANRNFGAE
                     GLPPMVVYIHGGPTSA"
     gene            1824..1895
                     /locus_tag="PKL60_06815"
     tRNA            1824..1895
                     /locus_tag="PKL60_06815"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1856..1858,aa:Lys,seq:ttt)
     gene            complement(2855..3907)
                     /locus_tag="PKL60_06820"
     CDS             complement(2855..3907)
                     /locus_tag="PKL60_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNW95881.1"
                     /translation="MQKTYTRSVWFALLAAALYALSAPLSKLLLNEMPPAMLAALLYL
                     GAGIGMFFVGMLKGADTNTQQRWELSRADSPYIIGMILLDIAAPLLLMNGLVRTSPAN
                     ASLLNNFEIVATTLIALLWFGEFVSRRLWAAIVLITIASILLTLQDSGSLQFSSGSLL
                     VLAACLCWGLENNLTRKLSFKNPLQVVVMKGLGSGSGAFIIAFLLHQTHWRLPAVLGG
                     LALGFVAYGLSIFFYISAQRQLGAARTSAYYAAAPFFGVALSFLIFRTPPSPLFLTAL
                     AVMLLGAYLTITEKHIHLHTHPAETHTHAHNHTDGHHTHTHEPMPEGEHSHEHTHEAL
                     EHEHSHLPDLHHLHKH"
     assembly_gap    4092..4191
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4192..5800
                     /locus_tag="PKL60_06825"
     CDS             <4192..5800
                     /locus_tag="PKL60_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95882.1"
                     /translation="WGRIEAKGASALRRHLMNMVSRHQRKPYLILIPGVLLALMLKAC
                     LPMENGDISGRPLVLQNFSAELTGKGTHGSVVCVRYPGRRADLESLGLQASLSRGGEM
                     IEAGIPAETLEGDTCFELTDAKYVSVSSNPEGGALRIPLDPLTLTLDALSPNGQPIEN
                     ASQTLSVGPYVQFPFLRWIFPEGVRPACIADGHTRSGVFYPAWDIIPEPSESCPTIVG
                     TPLLAPVDGTAYVWLLPNEDSSRRFDTVNALLFYSEDTGFAVDLTHAADLIFDGSRWT
                     MLKLLNGQKVSAGALVGMIGPKDHASSIPHIHLQVFIPPELPTGRAKDNETLYKASTS
                     ISTANVDAIKQGLFLDPGLKETLLSLASQSLRCEDYPWGELVVPQQLALNVDGQGEDW
                     AAYQPALSDKSGDSAAGETLDLRALYSAVDENYLYMMLEAGPQPEGKWAVDFFVDLKA
                     KNACGSSERSIKVWSDQPDGFTVGSVDNCPDSSAQTYPALFVWGEVLEVRIPLAYLNN
                     PDELRALSVKGILVDAEGGMSYPDYMR"
     assembly_gap    5961..6060
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6209..7192)
                     /locus_tag="PKL60_06830"
     CDS             complement(6209..7192)
                     /locus_tag="PKL60_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95883.1"
                     /translation="MGKFLESEKIEQIAWKATSPTISEQARGEGFFRGKFRPFCLPQQ
                     YAEENLFPPIREEALEFFREKRISWHQGSAGKPSSHLCDSQVCGVNFLFPFANRPDEL
                     ARLLKPIFPNIQRMLPVEDKRYVSFEWIGEENYLEERVQRDGVRTRGANFTSLDAIVM
                     FEDVNCKRQVVLIEWKYTESYSGNYLGIADSGTDRRQIYQHLFDNPACVVDLNKLPDL
                     DSLYYEPFYQFMRQQFLAAKMEEARELGADHVRLLHIAPRINLDFTKITSPKLIGLGK
                     SATEVWGNLVRVGENFISVKTEDLFLNFASAEMEDWNAYIRTRYRWVAHKE"
     gene            complement(7199..7882)
                     /locus_tag="PKL60_06835"
     CDS             complement(7199..7882)
                     /locus_tag="PKL60_06835"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW95884.1"
                     /translation="MNKEQHKRIRLKLLPLERYIGVDRKDPIRFYRLPVIGRLYQRRI
                     ELSLEQLSGGDSILEIGFGSGVSFPNLAEMYRHIHGLDLTADVTPIAQMFAGMGISAD
                     LRNGSVLEMPYADAQFDAVLLISILEHLQPADQAQAFAEILRVLKPGGQLVYGVPVER
                     PLMVFLFRLLGTDIRTHHFSTEEDVRQAAEKAFEEGHISPLRGPLGLFGQVYQVGNFT
                     KGAKVSKET"
     gene            complement(7917..>8179)
                     /locus_tag="PKL60_06840"
     CDS             complement(7917..>8179)
                     /locus_tag="PKL60_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95885.1"
                     /translation="DDVNRMIKARAEREVQTMLRELGLGGVDELKGLVQAKRQAEDAE
                     KSELQKANERAAQLEKQLADVAAKQKVLLTQIEYNHADRIYS"
BASE COUNT         1644 a         2236 c         2361 g         1738 t
ORIGIN      
        1 cgccgaagtt gggccgccgt ggatgtagac caccattggc ggcaaaccct ccgcgccgaa
       61 attgcggttg gcgggggcgt agtaggtgcc gtagacgcgc gctccgtcgg aagacagcca
      121 gtccacttcc tgcgcctgcg gcaggtcctc cgggtgcagg tcgtcggctc ggctgcgggc
      181 agcgatgcgc atctgacccg cttgcaggat gatgatgcgg gcgggcaggc tgggggactg
      241 ggcaatcagg gcaagcgcgc cgctggcgga aacgcagagc tgctccagat tagtgaatgc
      301 acggatgtct acagcctggc tctcgccgct gcggatgtcc actttccgca ggtacacttt
      361 ggcctgttgg ttttccagga aatacagggc gctgctctcg ggcgcccatg ccaaaacacg
      421 cgaaccctgc acccaggctg gcggcagcag ggaacgcccg tccaggagga tgcgtttctc
      481 gccggtttgc aggtcgaata aaaccagttg gtcccattcg cccgggttgc gcaggtaggc
      541 aagataacgt ccatccgggg aaaactcggg ttggaagatc ggggtttcgg ggccgccgtc
      601 caagagatga gcttcggcag gcgcgccgcc gctctcgtcc aacaccgcga acatcaggcg
      661 cgttccgtcc cagggcatgt cggggtggtc ccattccacc caggcggcgg ctttgccgtc
      721 cgggctccag gtcggctgca tataaaaatc tgcgccagaa gcaaacacgc gcggccagct
      781 tttcccatcc agccggacga gcgccagaac gtcgcgttct tcgtagctgt gcacgaaaag
      841 gacgcggtcc tgcgctgggg aaagctttgg gctggcgcac ctgccaaacg cgggcgtgag
      901 cgcttgcggg ctgccggggc cgtagggggc gaaatagaga cggccgtctt tttccgcaaa
      961 gactgccccg cccacgccgg cacaaaactc gccgccgccg tatccgatac tgccggaggg
     1021 gttgaagccg gcgctgagct ctgagggagc atccaacccg acttgcgtga gcaggctggc
     1081 tttcccgctg agggaaccgc accagaccag gcgatctgag ccgggcgccc attgcacgtc
     1141 ggaaaagcgc gcgctgccgc cgatcaattc aggcgtgatg ggtgaaagcc aggtgccaaa
     1201 gggtttgttc gttttagtag ggggcatggc gctgcttctt tcgctgaggc cggggaaaag
     1261 ccggcggatg ttttcgatat gttccgggta atggtcagtc acatcattgt gcaggacgtc
     1321 cgggatgaag acgggttccg gaacgccttc ccagatttgc gggcggaaga gctcctcttc
     1381 gggtagggtg gagagtgcat tccacaaact ctcgaacgcc gcgtgggaat cctgcaaggt
     1441 cttttcgacc gggtcgtctt tgtggcgttc gcggatgatg gggttttgct cctgccaggt
     1501 catgaagtcg gtatccacaa ctgtatgcgg gcggcgttcg agctgctccc ataagcgctc
     1561 ggccagctgg cgttcgtaga aagtgaggtg ggtgagcgtg tctttcgtgg accattcgcc
     1621 gccggggagg gtctcttcga agcgttcggg agggacagcg ttgacggtta tgtcaaaagc
     1681 ggcgcgggct gcgcgcagtt ggcggagcaa ttcgggtttg gtgaggggct gtaggtgtgg
     1741 cggcatcgtg tggtaatgat aacaccagag caggaaaaat cggttgactt agggggatgg
     1801 gcgtgctaaa ataatgccct gccgggcgcg tagctcaatg gtagagcagt ggccttttaa
     1861 gccattggtt cagggttcga gcccctgcgc gctcactgcc aatatttccc ccgtcagaga
     1921 tgcccccgga agcgggggtc tttgattcat cccttgcagg tgaaattgga aatcgaagcc
     1981 gctgtgctgc gttccccgct gttcctggcg cgccgggggc agattaaagg aaccagactg
     2041 gttttcctga ttggaaccca gaacctgctc aggtcttagc gcgtatgttt cagagcgcag
     2101 atttcccttc ctgcatcgcg cttttccgct cgcgaaatcg gctctctgct gcctgcaggg
     2161 agacgcgttg cgctgcggct atcgccgcag ccgaaaggga ttcagcgggc agaggcgcgc
     2221 cgccttgcgc tctccccata aaaagaacca ggtaatcaat ttttcaaaca aaccttgagg
     2281 catttgcctg aagtccattc tcaatcagca tatctttggc tcttcctttc agaatgagtt
     2341 ctcaggagta gtaaagcctg cagcatccca gccgccgtga gagttgccat cctcgctgct
     2401 gccgctgcct gcggcagcgt ctcagcatct ccggacgctg acgaatgatc catccgcatc
     2461 ccctatgtca aacccggcgc aaacggggat tgtaattgct gggcagctgc ctgcgacctg
     2521 caaaccgcgc tctcgcgctc ggcagccggc gatcaaatcc gggcggcggc aggcaggtct
     2581 aaacccaggg ccgcacgcaa tacttcccgg cggcttactg aggacgtgca aagcgcctga
     2641 gcaccggttc ccctgagttt cgctgttctc ctgaccgaac gaggcgcttg agcgcagatg
     2701 attaaagctg tcaacaaaga cgggaacatg tttggttcgg gcaattaatc cacaggcacc
     2761 agaaggtccg gggccttgag ggccctgacg aaaaatttct gaacccggcg tctctttgag
     2821 catggagacc gcctgtagtg gcaaccagcg cgactcaatg cttgtgcagg tggtgcaggt
     2881 ccgggaggtg gctgtgctca tgttccaggg cctcgtgggt atgctcatgg ctgtgctcac
     2941 cttccggcat gggttcgtgc gtgtgcgtgt ggtgcccatc cgtgtggttg tgggcgtggg
     3001 tatgcgtctc cgccgggtgc gtgtgcaggt gaatatgttt ctctgtgatg gtgaggtagg
     3061 ctcccagcag cataaccgcg agcgcagtca ggaagagtgg ggagggcggc gtgcggaaaa
     3121 tcaggaaaga gagcgcgacg ccgaagaatg gagccgccgc ataataagcg ctggtcctgg
     3181 ccgcccccag ctggcgctga gcgctgatat aaaagaagat gcttaaccca taagcgacaa
     3241 agcctaaggc cagccccccg agcacagctg gaaggcgcca gtgcgtctgg tgaagcagga
     3301 acgcgataat gaatgctcca gagcccgatc ccaggccttt catcacaacc acctgcaagg
     3361 ggttcttgaa agaaagtttg cgagtcagat tgttttccag accccaacag aggcaggctg
     3421 ccagcactaa caacgagccg ctcgaaaact gcaggctgcc cgaatcctgc aaggtcagca
     3481 gaatgctggc aatcgtaatc aggacgatag cagcccataa tcggcgggag acgaattcgc
     3541 caaaccagag aagcgcaatc agggtggtgg cgacaatctc aaaattgttt aacagagagg
     3601 cgttggccgg gctcgtccgc accagaccgt tcatcaggag caaaggggcg gcaatatcca
     3661 ggaggatcat accgatgatg taaggcgagt ccgcgcggct cagctcccat cgttgttgtg
     3721 tattggtgtc cgctccttta agcatgccca caaagaacat cccaatccca gcccccagat
     3781 agagcagagc agccagcatg gctggcggca tttcgtttaa aagtagtttg gacaacggcg
     3841 cgctgagggc gtaaagcgct gcagccagga gagcgaacca tacagaacgc gtataggtct
     3901 tctgcatctt ttcctttatt cccattcagt aagcccgcag gactgcgctt ggggcgggtc
     3961 aaaaaccctg ctgcgcgcct ggcggtatcg gcttgaggag cagctgccag agcaagtctg
     4021 gactgctatc gaacttccga agcttaagta taattgcagt atcgagcaat ggaaactgct
     4081 tgggttctac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttggggcag
     4201 aatcgaagca aaaggagcct ctgcgctccg gcggcatcta atgaatatgg tcagcagaca
     4261 ccaacgaaag ccttacctta tcctgattcc cggcgtgctc ctggcgctca tgctgaaagc
     4321 ttgtcttccc atggaaaatg gggatatctc cggaagaccc cttgtgctgc agaatttttc
     4381 cgcggagttg accggcaaag gcacccacgg cagcgtcgtg tgcgtgcggt accccggcag
     4441 gcgcgcggat ttggaaagtt tgggcctgca ggcttcttta agccgcgggg gagaaatgat
     4501 tgaagccgga attcctgcgg agacgctgga aggggatact tgttttgagc tgaccgatgc
     4561 gaaatacgtt tctgtgagca gcaacccgga aggcggggcg ctccgcattc ccctggaccc
     4621 gctaacgctt actctcgatg ccctttcgcc caacggccag cccatcgaga acgccagcca
     4681 gacgctctcc gtgggtccat atgtgcagtt cccattcctg cgttggatat tccctgaagg
     4741 agtgaggcct gcctgcatcg cggacgggca cacgcgcagc ggtgtattct atcccgcctg
     4801 ggatattatc cccgagccgt cagaaagctg cccgaccatt gtgggcacgc cgcttttggc
     4861 tccggttgac ggaacagcct atgtctggct gctcccgaac gaagactcct cccggcgctt
     4921 cgacacggtc aacgctctcc tgttctactc cgaagacacc ggctttgcgg tcgatctgac
     4981 gcacgcggcg gatcttatct tcgacggcag ccgctggacg atgcttaaac tgctgaacgg
     5041 gcaaaaggtc tctgctggcg cgctggttgg catgattggt ccgaaggacc acgcctcgag
     5101 tatcccgcat atccacttgc aggtgttcat cccgcccgag ctgcccacag gccgggcgaa
     5161 agacaacgag accctctata aggcttctac gtccatcagc acagccaacg tggatgcgat
     5221 caaacagggg ctctttctgg acccgggttt gaaggaaacg ctgctctcgc tggccagcca
     5281 gtctctgcgc tgcgaggatt atccctgggg agaattggtg gtgccacagc agttggcgct
     5341 gaacgtggat gggcaagggg aggattgggc tgcttaccaa cccgcgttga gcgacaagag
     5401 cggggattct gccgcggggg aaacgctgga tttgcgcgcg ctctacagcg cggtggatga
     5461 aaactacctg tatatgatgc tcgaggccgg tccgcagccg gaaggaaagt gggctgttga
     5521 tttcttcgtc gacctgaagg cgaaaaatgc ctgcggatcc agtgagcggt cgatcaaggt
     5581 ttggtcggac cagccggacg ggtttacggt gggcagcgtg gataactgcc cggattccag
     5641 cgcgcagact tacccggcgc tgtttgtctg gggggaggtt ttggaagtgc gtattcccct
     5701 cgcgtacctc aacaatccgg acgaactgcg cgctctctcg gtgaagggca ttctggtgga
     5761 tgcggagggt gggatgagct acccggacta catgcgctga gcttggtttc ccggaggagg
     5821 gtttgagttt ttcacgccga ttgtgtccgg atgatgaagt cctgcagcgc ttctaacgcg
     5881 accgggacga ttagcggggg taacgaagga tttttctcat aggtctcagg aatggaatta
     5941 ccggaaggtg ggagacctga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 gtttggagac ctgaaggtca agctccgcgg gcggcctgga gaccggcgcg aactttgttt
     6121 ggagacctga aggtcaagct ccgcgcgtag tctggagacc ggcgcgatct aagaaataat
     6181 ctgaaaattt tctttattgc gggataactt attccttatg agccacccat cgatatcttg
     6241 tacggatata ggcattccaa tcttccatct cggcactcgc aaaattgagg aatagatcct
     6301 ctgttttcac gctgataaaa ttttccccca ctctgaccag gtttccccaa acctctgttg
     6361 cactcttccc caggccgatt aactttggag acgtgatctt cgtaaaatcc aggtttattc
     6421 ttggcgcgat atggagcagc ctgacatggt ccgcccctaa ctcacgcgcc tcttccattt
     6481 ttgcagccag gaattgttgg cgcatgaatt gataaaaagg ctcatagtag agcgagtcca
     6541 ggtcgggtag tttattgaga tctacaacac aagcaggatt atcgaatagg tgttggtaaa
     6601 tctgtcttcg gtcagtgcca ctatccgcaa tccccaggta atttccagaa taggattccg
     6661 tatacttcca ttcaatcagt acgacctgcc ttttacaatt tacgtcctca aacatgacaa
     6721 tcgcgtccag gctggtgaaa tttgctcccc tggttcgcac tccatctcgt tgaacacgct
     6781 cttccagata attttcctca cctatccatt cgaaagatac gtatcgctta tcctcaactg
     6841 gcagcattct ttggatattc gggaaaattg gcttgagcaa tctcgccagt tcatccggtc
     6901 tgtttgcgaa tggaaacagg aagttcacac cacacacctg ggaatcacac aaatggctgc
     6961 ttggtttacc cgcgcttccc tggtgccagc ttattctttt ctctctgaaa aattcaaggg
     7021 cctcctcccg aattggagga aaaaggttct cttcagcgta ttgctgaggg agacagaaag
     7081 gtctgaattt tcctctgaaa aaaccttcgc ctcgcgcttg ttctgaaatc gtgggcgacg
     7141 ttgctttcca ggcgatttgt tcgatttttt cactttcaag aaatttcccc atatgctctc
     7201 acgtttcctt tgatactttc gcgcccttcg tgaaattccc gacctggtag acctgcccga
     7261 acagtcccag cggtccgcgc agcggggaga tgtgcccctc ttcgaatgcc ttttccgccg
     7321 cctgccgtac gtcctcttcc gtggagaaat ggtgggtgcg gatatccgtc cccagcagcc
     7381 tgaacaggaa caccatcagc ggcctttcca ccggcacgcc gtagaccaac tgcccgccgg
     7441 gcttgagcac gcgcaggatt tcggcgaagg cttgggcctg gtctgccggc tgcaggtgtt
     7501 cgaggatgct gatgagtaag accgcgtcga actgggcatc cgcgtagggc atctccagca
     7561 cgctgccatt acgcaggtca gcgctaatcc ccatacccgc aaacatctgc gcgattggcg
     7621 taacgtccgc ggtcaggtcc aggccgtgga tgtgccggta catttccgcc aggttgggga
     7681 agctgacccc ggagccaaaa ccaatctcga ggatggaatc cccgcccgac aactgctcca
     7741 gggaaagctc aatccggcgc tggtacagcc tgccgatgac cggcagccga taaaagcgga
     7801 tggggtcttt gcggtctacg ccgatgtaac gctcgagggg cagcagtttc agacggattc
     7861 gcttgtgctg ttctttattc atataccact ccgtttcgaa gccatgacgg taagaattac
     7921 gaataaattc tatcagcatg attgtattct atctgggtca gcaaaacctt ctgcttcgct
     7981 gccacgtccg ccagctgctt ctccaattgc gccgcgcgct cattggcctt ctgcagctca
     8041 gacttctcag cgtcctcagc ctggcgcttc gcctgaacaa gccctttcag ctcgtccacg
     8101 ccgcccagcc caagttcgcg cagcatcgtt tgaacctcgc gctctgcccg cgccttgatc
     8161 atccggttca cgtcatcct
//
LOCUS       DAOQAD010000082         8135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_10572_length_8135_cov_5.660520, whole genome shotgun sequence.
ACCESSION   DAOQAD010000082 DAOQAD010000000
VERSION     DAOQAD010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10572_length_8135_cov_5.660520"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="PKL60_06845"
     CDS             complement(<1..1357)
                     /locus_tag="PKL60_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNW95886.1"
                     /translation="MDLFDYSLANQLSQQAPLAARMRPKTLDDIIGQDHIIGPGTLLR
                     RAIEADRLFSSIIFYGPPGTGKTTIAQVISLLTQAEFVSLSAVLAGVADLREVIKDAA
                     ERRRLYRKRTILFVDEVHRWNKAQQDALLPHVESGLLTLIGATTQNPYFDVIKALVSR
                     SRIFELKPLTEADVLTLLRRALADPINGYGGRKIRADEDALLHLARTAGGDARSALNA
                     LELAVETTPLQDAELRITLDVAQESIQKRAVIYDKTDDAHYDTISAFIKSVRGSDPDA
                     ALYWLAKMLHAGEDPRFILRRLIILASEDIGLADPRAILVASAAAQNFEYIGLPEGVY
                     PIVEATLYLATAPKSNSAGAYFKALEEIEKNGAGPVPIHLMDPSRDAKGFGHGAGYDY
                     PHNHPGHWTPQQYLPDSVKGTRFYEPSEEGYEQEVAERVRKWREAQARALGGEGNGGP
                     EA"
     gene            1604..3265
                     /locus_tag="PKL60_06850"
     CDS             1604..3265
                     /locus_tag="PKL60_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNW95887.1"
                     /translation="MPRRFSSLMFISILLCAGLLAAAPAQPARADTYTVTNSSSSGAG
                     SLRQAILDANNHAGQDIIGFAIPTSDPGYSSALGVWFLRLSFVLPMLEDPAGVVIDGS
                     TQPGSSPDLPGIIIEHTTAVPVGVSLLKIISQDNTIKYLGFFNGRGDGVIIDGDQNVL
                     MHNQIFTSAGYGVHLLAGAEENSIDGNKICGHALDGIRLQSAHHNVINNNSVGEQPLY
                     LPSVGRNLGNGISLIQSDSNTISGNLISNNTLDGLLLSESDENQILQNTIGLNLSRTS
                     ELGNGGYGILLEQSAGNLVDETWISGNDKDGIRLTGANAAGNILQRNSIGASYSGPIP
                     NAQHGIGLYNGAHDNTIGSGSDGDLNNLILYNGWSGVAVVDSALGSNVIGNNFIYNHE
                     FYGIHINNSVSNALVANTIAGNGGIGDCAGVRIENSSGTEGAADINLIWDNSIRNNHG
                     KGIELVGGANNGIVAPVITSASCKNVLGIACPGCWVQVFSDDYDEGRVLEGVAVANSF
                     GVFSLSSSFTGPHLTAVAVDGPGNSSEFSLPALGCFRHWLPILMK"
     gene            complement(3435..4463)
                     /locus_tag="PKL60_06855"
     CDS             complement(3435..4463)
                     /locus_tag="PKL60_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNW95888.1"
                     /translation="MDENKTLEILSTVPLTEAQQARIKALGKHIHLTVSAGKSFADLP
                     KEAWEKAEVLFADGKQLPAREQVPALKWIQISLAGVDRALKQSLAADPGIVLTSASGV
                     MISQMGEYVLMALLMLGHKLPEAIALQKEHKWGNNASRSLQPLELRGSTVGIVGYGSI
                     GREVARLLYPLGVNVLAAKRDLMKLEDSGYTPVGLGDPMGHYFKRLYPIEGLKGMLKD
                     CDFVVLTLPLTPETKHLFSTEMFDAMKPGAYLVNVSRGDLIDENALAQAVRAGKVGGA
                     VLDVFSSEPLPAESPLWSLPEVIITPHISGASTHLVDDVVDLFVENLKRYQENQPLYN
                     VVDTGSGY"
     gene            complement(4463..5227)
                     /locus_tag="PKL60_06860"
     CDS             complement(4463..5227)
                     /locus_tag="PKL60_06860"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNW95889.1"
                     /translation="MPEPSYNAAELMIINAARLLKDGDQVFVGVGIPNLACNLARRTH
                     APNLQMIYEAGVIGARPNRLPLSIGDPTLVSGALSVCSMFEVFSLYLQRGNIDVGFLG
                     GAQIDQFGNINATVIGDYQRPKVRLPGSGGSMEIAAWANRCYIITPHQLRRFPARVDF
                     HTSAGFLEGGDARAKTGVRGAGPQAVVTNLGILKPDANGELVLAALHPGCNFEQAQAN
                     TGWALKQAETIEITPAPAEEELRILREELDPQRIYI"
     gene            complement(5229..6131)
                     /locus_tag="PKL60_06865"
     CDS             complement(5229..6131)
                     /locus_tag="PKL60_06865"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNW95890.1"
                     /translation="MSKLTSLSEAVRQHVHDGDLVYAAGFTHLIPFAAGHEIIRQGRK
                     NLVLARATPDLIYDQMVAAGCARKVIFSYSGNPGVGSLKWLRDGMQNGSLEWEEYSHF
                     GMISRLQAGASGLPFMPMRQTGALDLEKANPLYRRVQDPYGGEDVVVVPALCPDVAIV
                     HVQRADSEGNAHIWGILGEQREAAFAARRVILTAEEIVDADVIRSDPNRTLIPSLVVS
                     AVCHVPWCAHPSYTQGYYDRDNDLYLRWEEISKDEAGMQAYLDEWVYGVKDRAEYLSK
                     LGEETRERLKPGLRLSAPVNYGSY"
     gene            complement(6133..6390)
                     /locus_tag="PKL60_06870"
     CDS             complement(6133..6390)
                     /locus_tag="PKL60_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95891.1"
                     /translation="MKTLLRSFGRILTLVGLANLAWLLVNWWTGKAIGQLQILGLLVL
                     ALGLTLLRVHPQEKPAKAALSPSQREEEKHKHKSWIESGKN"
     gene            complement(6415..8001)
                     /locus_tag="PKL60_06875"
     CDS             complement(6415..8001)
                     /locus_tag="PKL60_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNW95892.1"
                     /translation="MTEATSFKLTYSTMFNPPEELHTRFAQGLEKVKTGLGREYPMLI
                     NGQERFSADKHYAHSPINTEMHLATFQKGTAQDAADAVAAAKAAFPAWSRMNWEERVY
                     LLRKAAENIDARIYEISAAMALEVGKNRMESLGEVAEAADLIRYACAQMEANGGFIRQ
                     MNSDPLSGFVSTNQSVLRPYGVWLVISPFNFPASLTCGPAGAALVSGNTVVMKPASDT
                     SWSPALLVDCMRQAGIPDGVFNFVTGPGSTLGEALINNPDVAGITFTGSYEVGMNIYC
                     NFGHSRYVRPTILELGGKNPVLVSRLADVEDAALGSVRSAFGLQGQKCSACSRVFIEE
                     PLYEAVTKRMVELTEKLSIGDPTELQTYMGPVINKKSYTDYQRFMEDLSAAGKVLVGG
                     KTLTEGALAKGYFCAPTIVADVPLSHPLWKQEMFVPIVMVSPVKDLQEGIALANDVQY
                     GLTAGFYGSPSESEWFFDHIEAGVVYTNRPQGGTTGAWPGFQPFGGWKGSGSSGKNSG
                     GLYYVPLYMREQIRNRVRRL"
BASE COUNT         1533 a         2398 c         2522 g         1682 t
ORIGIN      
        1 aagcttctgg ccctccattc ccctctcccc ccagcgccct ggcctgcgcc tcgcgccact
       61 tgcgcacccg ctccgccact tcctgttcgt acccctcttc tgatggttcg taaaaacgcg
      121 tgcctttcac gctgtccggc aggtactgct gcggggtcca gtgcccgggg tggttgtgcg
      181 gataatcgta gcccgcgccg tgcccaaagc ctttcgcgtc ccggctgggg tccatcaggt
      241 ggatgggcac cggaccggcg ccgttcttct caatctcttc cagcgcctta aaatacgcgc
      301 cggcggagtt ggatttgggc gcagtggcca ggtagagcgt ggcttccacg atcgggtaca
      361 cgccctcggg cagcccgatg tactcaaagt tctgcgcggc ggcgctggca accaggatgg
      421 cgcgcgggtc cgccaggccg atatcctcgc tggccaggat gatgagccgc cgcaggatga
      481 agcgcgggtc ctcgccggcg tgcagcatct tcgccagcca atacagcgct gcgtccgggt
      541 cggagccgcg cacggacttg atgaaggcgg agatggtatc gtagtgcgcg tcgtcggtct
      601 tgtcgtaaat caccgcacgc ttctggatgg attcctgggc gacgtccagg gtgatgcgca
      661 gctcagcgtc ttgcagcggg gtggtctcca ctgccagttc gagcgcgttc agtgcgctgc
      721 gcgcgtctcc tccggcggtg cgcgccaggt gcagcagggc atcttcgtca gcgcggatct
      781 tgcgtccgcc gtagccgttg atgggatccg ccagcgcgcg ccgcagcaag gtcaggacgt
      841 ccgcttccgt caggggcttc agttcaaaga tgcgcgagcg gctgaccagc gctttgatga
      901 cgtcgaagta ggggttctgc gtggtcgcgc caattagggt cagcaggccg gattccacgt
      961 gcgggaggag ggcgtcttgc tgagctttgt tccagcggtg aacctcatcc acaaacagga
     1021 tggtgcgctt gcggtacagc cggcggcgct ccgcggcatc cttgatgacc tcccgcaggt
     1081 ctgccacgcc ggccaacacc gcgctcaggc tgacgaactc tgcctgggtg agcagggaga
     1141 tgacctgcgc gatggttgtc ttgccggtgc cgggcggacc gtagaagatg atggacgaaa
     1201 acaggcggtc agcctcaatc gcgcggcgca ggagcgtgcc gggcccgatg atgtggtcct
     1261 gcccgatgat gtcgtcgagc gttttggggc gcatgcgagc agccaggggc gcctgctggg
     1321 aaagctggtt ggcgagggag tagtcaaaaa ggtccatggg atgattctat catagctatt
     1381 atgttctagg gttcatgggg aagctgccag aaaaacaagg ttttctgaag ccggcagagc
     1441 cttgtcccac gcaggaacag gcaagggctg gcaagtattc tatttttgtt ccgtgaaccg
     1501 gcacttgggc ggattccttt ggaaatattc cgaaaattcc ctatactgta gataaggtcg
     1561 tgcttcttcc agaatacttt cacaaccaat caggaggtat ctcatgccaa gacgtttctc
     1621 ctccctgatg ttcatcagca tcctgctctg cgcgggtttg ctcgcggctg ctcccgcgca
     1681 acctgccagg gcagacacgt acaccgttac caattccagc agctccgggg cggggtcttt
     1741 acgccaggct atcctggacg cgaacaatca tgccggtcag gatattatcg gctttgccat
     1801 cccgaccagc gaccccggat attcctccgc gttgggggtc tggttcctcc gcctgagctt
     1861 tgttcttccg atgctggagg acccagccgg cgtggtgatt gacgggtcga cccaacccgg
     1921 cagcagcccc gacctgcccg ggattatcat cgagcatacc accgccgtcc cggtaggggt
     1981 cagcctgctc aagattatct cgcaagataa cactataaaa tacctgggct tttttaacgg
     2041 ccgcggcgac ggcgtcatta tcgatgggga tcagaacgtg ctcatgcaca accaaatctt
     2101 tacctccgca ggctacgggg tccatttgct cgcgggcgcc gaagaaaata gcattgatgg
     2161 caataagatt tgcgggcacg ccctggacgg catccgcctg cagtccgcgc accacaacgt
     2221 gataaacaac aattcggtcg gggagcagcc gctttacctt cccagcgtcg ggaggaatct
     2281 cgggaatggc atcagcctga tccagagcga cagcaacacc atcagtggaa atctgatcag
     2341 caataacacg ctggacgggc tgcttttgtc ggaatccgat gaaaaccaaa tcctccagaa
     2401 caccatcggc ctgaatctgt cccggacaag cgagctgggc aacggcggct acggcattct
     2461 gttggaacag agcgccggca atctggtgga tgaaacctgg atttcgggca acgacaagga
     2521 cggcatccgc ctgacgggcg cgaacgcggc cgggaacatt ctccaacgca attccatcgg
     2581 tgcaagttat tctggcccta tccccaatgc ccagcacggg attgggcttt acaacggcgc
     2641 gcatgacaac accatcggca gcgggtcgga tggagatctc aataatctca tcctctacaa
     2701 cggttggagc ggggttgctg tggtggattc tgctctgggc agcaatgtta tcgggaacaa
     2761 ttttatctat aaccatgaat tctacggcat ccacatcaac aacagcgtaa gcaacgcgct
     2821 tgtcgcgaat accatcgccg gtaacggcgg cattggggat tgcgcgggcg tgcggataga
     2881 aaacagcagc ggcaccgaag gcgcggcgga tatcaatctt atctgggata acagcatccg
     2941 caacaatcac ggcaaaggta tcgagctggt cggcggcgca aacaacggga tagtcgctcc
     3001 tgtcatcacc agcgccagtt gtaaaaatgt tttaggtata gcctgtccgg gctgctgggt
     3061 gcaggttttt tcggatgatt atgacgaagg ccgcgtgttg gagggagtcg cagtcgcaaa
     3121 cagctttggc gttttctcgc tctccagcag ttttaccggg ccgcacttga cggctgtggc
     3181 ggtggacgga cccgggaatt ccagcgagtt ttcgctgccg gcgctcggct gcttccggca
     3241 ttggctgcca atcctgatga agtaggcagt tcctgcagcc cgcagctgta cgctgggacg
     3301 aagtccggga ttgaccctgg ggcaggcgtc ggggaactgg ttaatcctgc cgtaaacccg
     3361 tccctgagtt accgcctcaa ataaaaaagc ccgcgcgaat ccggcgcggg cctgtgcatc
     3421 gccttgaatg atggtcaata tccgctgccg gtatccacga cgttgtaaag cggctgattc
     3481 tcctggtagc gcttcaggtt ctctacgaac agatccacaa catcatctac caggtgggtg
     3541 gaggctccgg aaatgtgcgg ggtaatgatg acctcgggta agctccacag ggggctttcc
     3601 gcgggcagcg gttccgacga gaacacatcc aacaccgcgc cgcccacttt acccgcccgg
     3661 acggcctgcg ccagggcgtt ttcatcaatc aaatccccgc gggaaacgtt cacgagatac
     3721 gcgcccggtt tcatggcgtc gaacatctcg gtggagaaca ggtgcttggt ctctggcgtc
     3781 agcggcaggg taagcaccac gaaatcgcag tctttcagca tgcccttcag cccctcaatc
     3841 gggtaaagac gcttaaaata gtgccccatc ggatctccca ggccgaccgg cgtgtagccg
     3901 ctgtcttcca gcttcatcag gtcgcgtttg gctgccaaaa cgttcacgcc caaggggtat
     3961 aacagacgcg ccacctccct gccgatagac ccgtaaccca cgatgccgac tgtgctgccg
     4021 cgcagctcca gcggctgcag ggacctggag gcattgttgc cccatttatg ctccttttgc
     4081 agggcgatag cttcgggcag cttgtgcccc agcatcagca gcgccatgag cacatattcg
     4141 cccatctggc tgatcatgac cccgctggca gaggtgagca cgatgcccgg gtcagcagcc
     4201 agcgactgct tcagcgcccg gtctaccccg gccagtgaga tttggatcca tttaagcgcg
     4261 ggcacctgtt cgcgcgcggg cagctgtttg ccatccgcga agagcacctc ggctttttcc
     4321 caggcttctt tgggcagatc cgcgaaagat ttcccggcgc tgacggtcag gtgaatgtgt
     4381 ttacccagcg ctttgatgcg ggcttgttgg gcttccgtga ggggcacggt gcttaaaatt
     4441 tccagtgttt tgttctcatc catcaaatat atatcctttg cgggtccagt tcttcccgta
     4501 aaatgcgcag ttcttcctcc gcgggcgcgg gggtaatttc aatggtttcc gcttgcttca
     4561 acgcccagcc ggtgttggcc tgcgcctgtt caaagttgca gcccggatgc agggccgcca
     4621 aaaccagttc gccgttcgcg tcgggcttga ggatgcccag gttcgtaaca accgcctgcg
     4681 gaccagcgcc gcgcacgccc gttttcgcac gggcgtcccc gccttccaga aaaccagccg
     4741 aggtgtgaaa atccacccgg gcggggaagc gccggagttg atgcggggtg atgatgtagc
     4801 agcgattggc ccaggcggcg atttccattg aaccgccgga gcccggcagc cgcactttgg
     4861 ggcgctggta atccccgatg accgtggcgt tgatgttgcc gaactggtca atctgcgcgc
     4921 cgcccaaaaa gcccacgtcg atattaccgc gctgcagata gagcgaaaac acttcgaaca
     4981 tcgaacagac cgatagcgca ccgctcacca gggtcgggtc gccgatggaa aggggcaggc
     5041 ggtttggcct ggcgccgatg accccggcct cgtaaatcat ctgcaggttg ggggcgtgcg
     5101 tgcggcgggc aaggttgcag gccaggttcg ggatgccgac gccgacaaag acctggtcgc
     5161 cgtccttgag caggcgggcg gcgttgataa tcatcagttc ggcggcgttg tatgacggtt
     5221 ccggcatctc agtagctccc gtaattgacc ggcgcgctca ggcgcaggcc cggcttcagg
     5281 cgttcgcgcg tttcctcgcc cagctttgaa aggtattccg cccggtcctt cacgccgtaa
     5341 acccattcat ccaggtaggc ttgcatgcca gcctcatcct tgctgatttc ctcccagcgc
     5401 aggtacaggt cgttatcacg gtcgtagtag ccttgggtgt aagaggggtg cgcgcaccag
     5461 ggcacatggc agaccgcgct gactaccaat gaggggatca gcgtgcggtt agggtcggag
     5521 cggatgacgt ccgcgtccac gatctcttcc gcggtcagga tgacgcgcct ggcggcgaag
     5581 gcagcctcgc gctgttcgcc caggatgccc cagatgtgcg cgttgccttc gctgtccgcg
     5641 cgctgcacat gcacgatggc gacgtccggg caaagcgccg gcactaccac cacatcttca
     5701 ccgccgtagg ggtcctgaac gcggcggtag agcggattgg ctttctcaag gtccagagcg
     5761 ccggtctgac gcatgggcat gaagggcagc ccgctggcgc ccgcctgcag gcggctgatc
     5821 atcccgaaat gggagtattc ctcccattcc aggctgccgt tttgcatgcc gtcgcgcagc
     5881 cacttgagcg agcccacgcc cgggttgccg gagtacgaaa agatgacctt gcgggcgcag
     5941 cccgcggcca ccatctggtc gtaaatcagg tcaggggtgg cacgcgccag gacgaggttt
     6001 ttccttccct ggcgaatgat ttcgtgcccg gcggcaaaag ggatgaggtg cgtaaagccg
     6061 gcggcataca caaggtcccc gtcgtgcacg tgctgtcgga cggcttcgct cagactggtc
     6121 agtttggaca tgctaatttt tcccgctttc aatccaggat ttgtgtttgt gcttttcctc
     6181 ctcgcgttgg gagggggaaa gagccgcttt ggcaggcttt tcctgcgggt ggacgcgcag
     6241 cagggtgagc cccaaagcca acaccagcag ccccagaatt tggagctgac cgatggcttt
     6301 gccggtccac cagttcacca gcagccaggc aaggtttgcc agcccgacca gggtcaggat
     6361 gcgtccaaag ctgcggagaa gggttttcat gcgcgcccgc gggtcggatt gggtttacag
     6421 gcggcgtacg cggttgcgga tttgctcgcg catgtacagc ggtacgtaat acagcccgcc
     6481 gctgttcttg ccgctggagc ccgagccttt ccagccgccg aagggctgga agccgggcca
     6541 ggcgccggtg gtgccgccct gggggcggtt ggtatagact acgccggctt cgatatggtc
     6601 aaagaaccat tcggactcgg atgggctgcc gtagaagccg gcggtgagcc cgtactggac
     6661 gtcattggcc agcgcgatgc cttcctgcag gtctttgacc gggctgacca tcacaattgg
     6721 cacgaacatt tcctgcttcc aaagtggatg cgagagcggc acgtctgcca caatggtggg
     6781 ggcgcagaag tagcctttgg caagcgcgcc ttccgtcagg gttttgcccc cgacgagcac
     6841 tttgcctgcc gcgctcaggt cttccataaa gcgctggtag tcggtgtagc tcttcttgtt
     6901 gatgaccgga cccatatacg tctgcagctc ggtcggatcc ccgatagaga gcttttcggt
     6961 gagttccacc atgcgtttgg tcacagcctc gtagagcggc tcttcgataa agacgcgcga
     7021 acaggccgaa cacttctgcc cctgcagccc gaaggcggag cgcacactgc ccagggcggc
     7081 atcttccaca tctgcgaggc gggaaacaag cacggggttc ttgccgccca gctccaggat
     7141 ggtcgggcgc acgtagcgcg agtgcccgaa gttgcagtaa atattcatcc caacctcgta
     7201 cgagccagtg aaggtgatgc cggccacgtc cggattgtta atcagcgctt cgcccagcgt
     7261 ggagccggga ccagtgacga aattgaacac gccgtcggga atgcccgcct ggcgcatgca
     7321 gtccacgagc agcgcaggcg accagctggt atcgctggca ggtttcatca cgacggtatt
     7381 gcctgatacc aatgccgcgc cggcaggccc acaggtcagg gaggccggaa aattgaaggg
     7441 gctgattacc aaccacacgc cgtaggggcg caggaccgat tggttggtgg agacgaaacc
     7501 cgagagcggg tcgctgttca tctggcggat aaagccgccg ttcgcctcca tctgcgcgca
     7561 ggcatagcgg atgaggtctg ccgcttcggc gacctctccc agcgattcca tgcggttttt
     7621 gcccacctcc agcgccattg ccgcggaaat ttcgtagatg cgcgcgtcga tattttcagc
     7681 ggccttacgc agcaggtaca cgcgctcttc ccagttcatg cggctccagg cggggaaggc
     7741 ggctttggcg gccgcaaccg cgtcggcggc atcctgggct gtgccttttt ggaaggtggc
     7801 cagatgcatt tcggtgttga tgggggagtg ggcgtagtgc ttatccgccg agaagcgctc
     7861 ctgcccgtta atgagcatcg ggtattcgcg cccgagcccg gttttgactt tttcgagtcc
     7921 ctgcgcgaag cgcgtatgca gttcttctgg gggattgaac atcgtgctgt aggtgagttt
     7981 gaaggacgtc gcttccgtca taggtcagaa tcctccggga atatgagatt gttttggtga
     8041 ttgtatcata gatggactca tcggctggcg aagtgtagag cgggggtccg aagcttgcgc
     8101 ggagtgcttt tcgtaggtcg ggttgcgcat tacaa
//
LOCUS       DAOQAD010000083         7898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_11021_length_7898_cov_5.574525, whole genome shotgun sequence.
ACCESSION   DAOQAD010000083 DAOQAD010000000
VERSION     DAOQAD010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11021_length_7898_cov_5.574525"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(354..1136)
                     /locus_tag="PKL60_06880"
     CDS             complement(354..1136)
                     /locus_tag="PKL60_06880"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNW95893.1"
                     /translation="MRFAVNYSVPMEKLLREGAVDCGLLKCPEWEGVISAARPLRPVH
                     VHFEIAVGSGGFGHLDFDLIRSLLASTETPHLNCHLMGSPELDPGSPADQRKLIGNWV
                     AEMEYLKQQVPGVKLAAENLPYIMEYYGSKIGSSADLIARAIREADAGLLLDLSHARI
                     SCHYLGMDLKEYVARLPMDRLSELHITGLQYYCGYLNDHFAMRPEDWEAAEWARAQIR
                     AGAWREPEIAAFEYGGVGDVFGWRTEERVLRQQVPRLAELFA"
     gene            complement(1149..2978)
                     /locus_tag="PKL60_06885"
     CDS             complement(1149..2978)
                     /locus_tag="PKL60_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95894.1"
                     /translation="MSTYYDENELDDNVPVKTNVLKRLVNTLRPHWKTLLGGVLAIVV
                     VSLLDAYFTILSKRIIDEGIIPQNKAAILHFFTIYGAIVLIQAVGAFLFIFLAGLQGE
                     RVRYDLRKQLFNHLQELSLSYFSKTPLGWIMSRVTSDTEKMAELLTWGIIDTTYALVS
                     IIVSSVFMFTINWQLALIVMFSLPVMAYAALKFRVRIYHHYRLSRKANSKMTASLNEN
                     ITGVRVVKALRREDRNLQDFKVLSSAMYRSSYRAAYLSALFQPTIQMISAISLGLILW
                     RGGIKVELGLITIGGLQAFVSYIMMILWPIQDLARVYADMQNAVASSERVFSLLDTQP
                     GITNRPAAREIPSLAGDIAFEGVSFRYEDDEPVIRNLSFHIPYGQSVALVGPTGGGKT
                     TIVNLLCRFYEPTEGVIRIAGVDYMDLSLQTIQSRIGVVLQTPHLFSGSIRENIRYGK
                     LDATDEEIEDAAKLAGAHNFITHFEHGYDQNVGEGGNLLSVGQKQLISIARAILSTPD
                     IFVMDEATSSVDTLTEALIQNGMEKLMTGRTSFIIAHRLSTIKNANRILVIRDGQIAE
                     SGNHNELMLLKGHYYRLYTQQFRYELETKLDPYGEVPNATDAI"
     gene            complement(2962..4791)
                     /locus_tag="PKL60_06890"
     CDS             complement(2962..4791)
                     /locus_tag="PKL60_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95895.1"
                     /translation="MMKKQQKKYPALDPQAVVASKNGLTALWRLMSGYHGHYLLSALM
                     LAISATARAGIYIFLGWFVDHILKEGRFGSELLLSAAGFVCLILVQATSGFLSNQLAA
                     QTAESSTRRLRDLLFDHIQRLSFAYHAEAKTGDLLERASSDVDTLRRFISDQAIGVAR
                     VIAIFIINFTAIMQINARLALVSIIIIPVILVVSILFFRKLSKAYEAYQEQEAVISTN
                     LQENLSGVRVVKAFARQAYEIEKFDANNFEKYKRGRKLNLMHSLFWPVSDILCAAQMI
                     GSNIMAAFMAIDGVITLGNYMSFTGLLGWLIWPIRNLGRLIVDTSRALVSLKRVTDVL
                     RVAQEPLLEGTHVPEQGVAGSIRFEHVSFAYEKDRPVLEDISFECPAGQIVALLGSTG
                     SGKTSLVNLLPRFYDVTEGKILLDGVDLRDYPRHYLRSQIGIVEQEPFLFSCSIRDNI
                     TYGITREVSQEEIEQAAREAAIHDVIMEFANGYDTLVGERGVTLSGGQKQRVAIARTL
                     LINPRILILDDSTSSVDMETEVQIRQALQALMQNRTTFIIAHRIQSIMSADLILVFDQ
                     GKIVQSGTHDELIQQPGIYRSIYEIQTRIDNALQEEIDRVHVL"
     gene            complement(4939..5715)
                     /locus_tag="PKL60_06895"
     CDS             complement(4939..5715)
                     /locus_tag="PKL60_06895"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNW95896.1"
                     /translation="MRVTESMKNAHLDGDSFLLEGGSTGAVLFHGLTATTAEVRLLGE
                     HLHKLGYTVSAPLLPGHGTHPDELNRVKWMAWYETAEAAFLEMRVRCQKVFIGGESMG
                     ALLALLLASRYEQVSGIVLAAPALILRNLKAAYLLQYFMKHLDKHQTEDFLPWKGYNV
                     YPVKSLVQLQKLQNVVRKELPAVTQPLLVFMGAKDKTVSPEVGKTIISRVASTDKRLV
                     NMPESPHVMLLAQERESIFAQIDEFIRELSETDRETRASA"
     gene            complement(5799..6629)
                     /locus_tag="PKL60_06900"
     CDS             complement(5799..6629)
                     /locus_tag="PKL60_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95897.1"
                     /translation="MKKTLTIVTVLLLLSACNVPNASEPTQDIIGTAVAVALTNAPTS
                     TPVPPTAALPTAQPIPPTETATSIPSATPTETPTPTPTLNPEDPALQYGSPDKVEDFS
                     SATGAWNYEDDWSNWNVNDGYLNIYSKGTPYWHSWYTTGTPIQNFYLEAELSMPNCSG
                     KDRIGLAFRLTEGSRFYFMGLTCDGTWGFNRYTASNASETILTYQSSDKIRPANENNR
                     IGVLANGNSFAFYINGALVGSTTDDTYPAAGTTGFVTMSAGTVNFTTRVDVLRYWLLP
                     "
     gene            6927..7184
                     /locus_tag="PKL60_06905"
     CDS             6927..7184
                     /locus_tag="PKL60_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95898.1"
                     /translation="MIQIKSLSVTTLKFLVLGDILADQATHISVCTSFPGGIGMGRIE
                     PLEAGKGASVKHHAKNETGGLVSGKNIHSSGHFYFALTISP"
     gene            7295..>7898
                     /locus_tag="PKL60_06910"
     CDS             7295..>7898
                     /locus_tag="PKL60_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNW95899.1"
                     /translation="MNFKVVSEAREKGTYPYFIPLDKNEGTEVLYEGRRIIMCGSNNY
                     LGLTTHPKVRKAAMDAIAEYGTSCTGSRFLNGNMTLIEDLERELAEWVGKEAALVFST
                     GMQVNLGTISALVGKSDIVILDKEDHASIVDGALLSGGKIERFRHNDMEHLERVLKSL
                     PEDKGKLLVVDGLFSMEGDIAPLPEIVPLCERYGVRLMRAD"
BASE COUNT         1738 a         2075 c         2320 g         1765 t
ORIGIN      
        1 tccgtcgctg gcgctccttc gcaatgacga atccgctgtg ctcgcgggct tgtgaagcga
       61 tctcccaggt ggcctgactt agacttacag gtcagcataa gaccgacagg cagattgctt
      121 ccgtcgctgg cgctccttcg caatgacgaa tccgctgtgc tcgcgggctt gtgaggcgat
      181 cacccaggtg gcctgacttt aggcttgcca atcggcatag gattgacagg cggatcgcac
      241 aaggggggat ttgctaggcc aggccgtttt gcctgactgt ccacatttgt tggtgtgaaa
      301 tctgaaaaga aaacggctcg caacaacgat ttgcacgtca ttgcgcccag ctctcacgca
      361 aacaactctg ccaggcgcgg cacttgctgc cggagcaccc gttcttccgt gcgccagccg
      421 aacacgtcgc caacgccgcc gtactcaaaa gcggcgattt ccggctcgcg ccaggcgccg
      481 gcgcgaattt gcgcccgcgc ccattccgcg gcttcccaat cctcagggcg catcgcaaag
      541 tggtcgttca gatagccgca gtaatattgc agtccggtga tatgcagctc tgagagcctg
      601 tccataggca ggcgcgccac atactcctta aggtccatgc cgaggtaatg gcagctgatg
      661 cgcgcgtgcg aaaggtccaa gagcaggccg gcatccgctt ccctgattgc ccgggcaatc
      721 aggtccgcgg acgaaccaat cttactgccg tagtattcca tgatataggg caggttttcg
      781 gctgccagct tgacgccagg gacctgctgc ttaagatatt ccatctccgc aacccagttt
      841 ccaatcaact tgcgttggtc tgccgggctg ccagggtcca gctctggaga tcccatcaga
      901 tggcagttga ggtgcggagt ctccgtgctg gcaagcaacg agcggataag gtcaaagtcc
      961 aggtgcccga agccgccgct gccgacggca atttcgaagt ggacgtgcac cggccgcagg
     1021 ggtctggcgg cgctgatgac gccttcccat tcagggcact taagcagccc gcagtccacc
     1081 gcgccttcgc gcagcagctt ctccatcggg accgagtaat tgaccgcgaa acgcaccttc
     1141 aggctccctc agatagcatc cgtcgcgttt ggaacctcgc cgtacgggtc aagtttcgtc
     1201 tccagttcgt agcggaactg ctgggtatag agccggtagt agtgcccttt caaaagcatc
     1261 agctcattgt ggttgccgct ttccgcgatt tggccgtcgc ggatgacgag gatgcggttc
     1321 gcgtttttaa tcgtagagag gcggtgcgcg atgatgaagc tcgtgcgccc ggtcatcagc
     1381 ttttccatcc cgttttggat gagcgcttcg gtgagcgtat ccaccgaact ggtggcctcg
     1441 tccatgacga agatatccgg ggtggagagg atggcacggg cgatgctgat gagctgtttt
     1501 tgcccgacgg agagcaggtt gccgccttca cccacgttct ggtcataacc gtgttcgaaa
     1561 tgcgtgatga agttgtgcgc gccagccagc tttgccgcgt cttcaatctc ttcatccgtt
     1621 gcgtccagtt tgccgtaacg gatattctcg cgaatgctgc cggaaaagag gtgtggggtt
     1681 tgcagaacca cgccaatgcg ggattgaatg gtctgcaggg acaggtccat gtaatccacg
     1741 ccggcaatcc ggatgacgcc ttccgttggt tcataaaagc ggcacagcag gttcacgatg
     1801 gtcgttttcc cgccgccggt cgggccaacc agggctactg actgaccgta tgggatgtgg
     1861 aagctgaggt tgcggatgac gggttcatcg tcttcgtaac ggaaggacac tccttcgaaa
     1921 gcgatatccc ccgccagcga tggaatttct ctggcagccg ggcggttggt gatgcccggt
     1981 tgggtgtcca atagcgaaaa tacgcgctcc gaggaggcca ccgcgttttg catatccgcg
     2041 tacacgcgcg caaggtcttg aattggccag agaatcatca tgatatagga aacaaaggct
     2101 tgcagaccgc caatcgtaat gaggccaagc tccactttga tgccgccgcg ccacaggatt
     2161 aagcccaggg aaattgcgct gatcatctga atggtcggct ggaaaagcgc cgagagatag
     2221 gccgcgcggt agctggatcg gtacatggca gaggagagca ctttgaaatc ttgcaggttg
     2281 cggtcctcgc ggcgcagggc tttgaccacg cgcacgccgg tgatgttctc gttcaggctg
     2341 gcggtcattt tcgagtttgc tttgcgcgac aggcggtagt gatgataaat gcgcacgcgg
     2401 aacttcaagg cggcgtaagc catcaccggc agcgaaaaca tcacaatcaa cgccaactgc
     2461 cagttaatcg tgaacatgaa taccgaggaa acgatgatgc tcaccaaagc gtaggtggta
     2521 tcgataatgc cccacgtgag cagttcggcc attttttccg tatccgaggt cacgcgcgac
     2581 atgatccacc cgagcggggt cttgctgaag taggacagcg agagctcttg caggtggtta
     2641 aacagctgtt tgcgcaggtc gtagcgcacg cgttctccct gcaagccagc caggaagatg
     2701 aaaaggaatg cgccgacggc ctggatcagt acaatcgcgc cgtagatagt gaagaagtgc
     2761 aggatggcgg ccttattctg cgggataatg ccctcatcga tgatgcgttt gctgagaatg
     2821 gtgaagtacg catccaacag cgaaacgacc acaatcgcaa gtaccccgcc aagcaaggtt
     2881 ttccaatgcg ggcgcagtgt attgaccaac cgctttagca cattggtttt tactggaaca
     2941 ttgtcatcca gttcgttttc gtcataatac gtggacacgg tcaatttcct cttgcagagc
     3001 gttgtctatg cgcgtctgaa tctcataaat gctgcggtaa atgcccggct gttgaatcag
     3061 ctcgtcatgc gtgccggact gcacaatttt tccctggtca aagaccagga taaggtcggc
     3121 gctcatgatg ctctggatgc ggtgcgcgat gataaaggtc gtacggtttt gcataagcgc
     3181 ctgaagcgcc tggcggattt gcacctcggt ttccatatcc acggaggagg tcgagtcatc
     3241 caggattagg atgcgcgggt ttatcagcag cgtgcgggcg attgccacgc gctgcttctg
     3301 cccgccggaa agcgtcacgc cgcgttcgcc cactaacgtg tcgtaaccgt tggcgaattc
     3361 catgatgaca tcgtgaatgg ctgcttcgcg ggcggcctgc tcaatttctt cctggctgac
     3421 ctcacgcgtg atcccgtagg tgatgttgtc gcggatggag caggagaaga ggaatggttc
     3481 ctgttcaaca atgccaatct gagagcgcag gtaatggcgg gggtaatcgc gcaagtcaac
     3541 accgtcaagc aggattttgc cttccgtcac gtcgtaaaag cgcggcagca aatttaccag
     3601 cgaggttttg ccggagccgg tggaaccaag cagcgcaaca atctgtcccg ccggacattc
     3661 gaaggagata tcctccagca ccgggcggtc tttttcgtag gcgaagctga catgctcgaa
     3721 gcggatactg cctgcgacgc cttgttcggg tacgtgcgta ccctcaagca gcggttcctg
     3781 agccacgcgc agcacatcgg ttacacgttt gagggaaaca agcgcgcggg aagtatccac
     3841 gatcaacctg ccgaggttgc ggataggcca gatcaaccag ccgagcagac cggtgaagga
     3901 catgtaattg ccgagcgtga tgaccccgtc aatcgccatg aaggcagcca tgatattgct
     3961 gcctatcatt tgcgccgcgc agagaatgtc ggagaccggc cagaagagcg agtgcatcag
     4021 gttcagcttc ctgccgcgtt tgtacttttc gaagttattc gcgtcgaact tttcgatttc
     4081 gtatgcctgg cgggcgaacg ctttgaccac ccgcaccccg ctcaggtttt cctgcaggtt
     4141 ggtcgagatg acggcctctt gctcctggta ggcttcgtag gctttggaaa gcttccggaa
     4201 aaacaggatg gaaacgacca ggatgaccgg aatgatgatg atagaaacca aagccaagcg
     4261 cgcgttgatc tgcataatcg cggtaaagtt gatgataaaa atggcgataa cgcgcgctac
     4321 gccgatggcc tggtcgctga taaagcggcg caaagtatcc acgtcggagg aggcgcgttc
     4381 gagcaggtcg ccggtttttg cttccgcgtg gtaggcgaag gaaagccgct ggatatggtc
     4441 gaagagcagg tcgcgcagcc ggcgggtgct gctttcagcg gtctgtgcgg ccagctgatt
     4501 gctgaggaag ccggaagtgg cttgcacgag aatcaggcac acaaagccgg ccgcggagag
     4561 gagcagttcg ctcccgaaac ggccctcttt gaggatgtga tccacgaacc agcccaagaa
     4621 gatgtagata cccgcgcggg cagtagcgga aattgccagc atcaaggcgg agagcagata
     4681 gtgcccatga tagccggaca tcagccgcca aagcgccgtc agaccgtttt tactggcaac
     4741 gaccgcttgc gggtcaagag ccgggtattt tttctgttgt ttcttcatca tgcttttccc
     4801 atgtactgca gtcgacgata ctcttgaaat gcagacaaaa tggcagtata cacgcgcacg
     4861 ctgagagcgt caattgactt tgcaaaaaag ggcagtcggt gatggggatt tatgtgtttg
     4921 gctgctgtcc gcgggctttc acgctgaagc ccgcgtttct cggtcggttt cgctgagctc
     4981 gcggatgaac tcgtcaatct gcgcaaagat actctcgcgt tcctgagcca gcagcatcac
     5041 gtgcggggat tccggcatgt ttaccaagcg tttatccgtg gacgcgaccc gactgatgat
     5101 ggttttacca acctccggtg aaacggtttt gtccttcgcg cccataaaca ccagcagcgg
     5161 ctgggtcacc gccggaagct ctttccgcac aacattctgg agcttctgga gctgcaccag
     5221 gctcttgacc ggatagacgt tgtaaccttt ccagggcaga aagtcttccg tctggtgctt
     5281 atccaaatgc ttcataaagt attggagcag gtaggcagct ttcaggttgc ggaggatgag
     5341 cgcgggcgct gccaggacga tgcccgagac ttgttcgtag cggctggcaa gcagcagggc
     5401 aaggagagcc cccatggatt ctccgccgat gaagaccttc tgacagcgca cccgcatttc
     5461 cagaaaagcc gcttcagccg tttcatacca tgccatccat tttacgcggt tcagttcatc
     5521 cgggtgcgtg ccgtggccgg gcagcagcgg cgccgagacg gtatatccca gcttatgcag
     5581 gtgttcgccc aacaggcgca cctccgctgt agtagccgtc aggccgtgaa agagtactgc
     5641 gcctgtgctg ccgccctcca gcagaaaact atctccgtcc agatgcgcgt ttttcatgga
     5701 ttcggtaact ctcatgctgc agccccttca aagtgatatt aaaaggaaag gcgtaagtga
     5761 ttcatcctta cgacttccgg ataagcgctg ccgaaaaatc agggcagcag ccagtagcgc
     5821 aagacatcca cccgggttgt gaagttcact gtacccgcgg acatcgtgac aaagcccgtc
     5881 gtgccggctg cggggtaggt atcgtcagtg gtggaaccaa ccagcgcgcc gttgatataa
     5941 aaggcgaagc tgttcccgtt ggcgagcacg ccgatgcggt tattctcatt cgccggacga
     6001 atcttgtcgg agctttggta ggtgaggatg gtctccgagg cattggaggc cgtgtagcgg
     6061 ttgaaacccc aggtgccgtc gcaggtgagg cccataaagt agaagcggct gccttccgtc
     6121 aagcggaaag ccagaccgat gcggtctttg ccggaacagt tgggcatgct caattccgct
     6181 tccaggtaaa agttctgtat cggcgtaccg gtggtgtacc agctgtgcca gtagggcgtg
     6241 cccttggagt agatattcag ataaccatcg tttacgttcc agttcgacca gtcgtcctcg
     6301 taattccatg ctccggtagc gctggagaaa tcctccacct tatccgggct gccgtactgc
     6361 agcgccgggt cttccgggtt cagcgtgggt gtgggggtag gggtttcggt gggtgtcgcg
     6421 cttgggatgc ttgtggcggt ttcggttggg gggatgggct gtgcggtagg cagggcggct
     6481 gtgggcggca ctggcgtgct ggttggcgcg ttggtcagag cgactgccac ggcagtaccg
     6541 atgatgtcct gcgtaggctc ggatgcgttt ggcacgttgc aggcgctgag gagcaagagc
     6601 acggtaacaa tcgttaaagt ttttttcatg tccgttcctt tgtaaaaaaa cctgacctgt
     6661 ctgatgcggt atatccacaa taagtatagc atggatgcat gcaggccttt ttgggtttta
     6721 acggtgcaag agcaaaggga aaatatcctg aaacagcaaa atcgaagggc cctcattcag
     6781 attaagagct tcgaaatcag cattcgattc ctccaagttc ttttcccact tggattcaac
     6841 tcgcaaatgc aacagactat tcaaagaata cctcaaaaga taactgaaaa tcaaggcatt
     6901 ttccaggacg aaaaatcaag cggttcatga tccagatcaa atcactttct gtaacgacct
     6961 taaaatttct ggtccttggg gatatattgg cggatcaggc cactcatatt tctgtttgca
     7021 cctctttccc aggcggcata ggaatgggca gaatagagcc gcttgaagct gggaaaggag
     7081 caagcgtgaa acatcacgca aaaaatgaaa cagggggctt ggtttcgggg aaaaacatcc
     7141 actcatcagg acatttctat ttcgccttga ctatttcgcc ttgactattt cgccttgacg
     7201 ctgtgtttta gcgggatggg ttataatcgc cgtagtcttg tttcaattat catctgattt
     7261 gaaagcgtgt gaaatggata tctttacgaa gtgtatgaac tttaaggtcg taagcgaagc
     7321 ccgggaaaaa ggcacttatc catattttat tcccctggat aaaaacgaag gcacggaagt
     7381 actgtacgaa ggccgccgga ttatcatgtg cggttccaac aactacctgg gtctgaccac
     7441 gcaccccaaa gtgcgcaagg cggcgatgga cgccatcgcg gaatatggca ccagctgcac
     7501 cgggtcgcgt tttctgaacg gcaacatgac cctgattgaa gatttggagc gggaactggc
     7561 ggaatgggtg ggcaaggaag ccgccctggt gttctccacc ggcatgcagg tgaaccttgg
     7621 gaccatcagc gcgttggtcg gcaagagtga tattgtcatt ctcgataaag aagaccacgc
     7681 cagcattgtt gatggcgctt tattgagcgg cggcaaaatt gaacgcttcc gccacaatga
     7741 tatggagcat ctggagcgcg tgttgaagag cctgccagaa gataaaggca agctgctggt
     7801 ggtggacggc ttattcagca tggaaggcga tatcgcccct ctgccggaaa ttgtgccgtt
     7861 atgcgaacgc tacggcgtgc gcctgatgcg cgccgacc
//
LOCUS       DAOQAD010000084         7822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_11179_length_7822_cov_6.215913, whole genome shotgun sequence.
ACCESSION   DAOQAD010000084 DAOQAD010000000
VERSION     DAOQAD010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11179_length_7822_cov_6.215913"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PKL60_06915"
     CDS             complement(<1..819)
                     /locus_tag="PKL60_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95900.1"
                     /translation="MSIARFLNLVKARFDSELAKAVISAFRAEPLVWKALTDEGLLNA
                     FEEFSGEDPKLWQPGLLAVFTLSPKLAAQNLLDQEVKIPTALNTKATKTLDTIRLTGL
                     EPSTLEEAALLALALRNYHALNNGWEDVSSFLSAPRGNLDLWRSAFACLPALLPQLPQ
                     LAQDLAAKTPLAQTGKVCALLLHMVDCTPTLENDRFQLLIDLFGTSSLDFQRECLQQL
                     KAVESPEFLGRLAASFLAAFPQEPPAEKENFKAPSFEKVIELRKIAFLKQAAGQP"
     gene            complement(848..1180)
                     /locus_tag="PKL60_06920"
     CDS             complement(848..1180)
                     /locus_tag="PKL60_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95901.1"
                     /translation="MISTTNQVVQLISYILFGLGVLCLILSIVILARQAMGKNIQTIA
                     NQTTKLAEKGITDGVSGLVGNASLLISSLNDLSRSNTGIGIFFVFLSLVLLAAAYFFI
                     KSFIGIVP"
     gene            complement(1242..2426)
                     /locus_tag="PKL60_06925"
     CDS             complement(1242..2426)
                     /locus_tag="PKL60_06925"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNW95902.1"
                     /translation="MTATVLVVDDEETARLVVSEFLRSKGYEVLSAGTLGEARQFINK
                     GQADIIILDVRLPDGYGPNLLTETARMVNRPQIIIITAHGEISMAVEAMKNGAQDFIE
                     KPLDLLELEKSVRRAGETVAMHRELNHLRAAQAGQEEFIVGETPAMKQIIDHARRTAE
                     SGISALITGPTGTGKEVLAHYIHKIGVRSGKPFVDINCPAIPSTMFESELFGHEAGAF
                     TGATHKKVGLMEVADDGILFLDEIASMPMDLQAKLLRAIEERSFRRLGGNILINVDVQ
                     ILAASNRNLKEMIANGSFREDLYYRLKVSDLDLPPLSARKQDIPDLVGYFLRKFTLRS
                     GSQITDVTPRAMKALMDYSWPGNIRELSNVMERAVIFCDDPVIDLNHLSSDVLSAVEP
                     GT"
     gene            complement(2433..4364)
                     /locus_tag="PKL60_06930"
     CDS             complement(2433..4364)
                     /locus_tag="PKL60_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW95903.1"
                     /translation="MANPISPQLPFDPAEDDLQALDFALILELLPSAALAYNRSSDVI
                     ISVNQKLQDLSSFEPLALTGKSLSALISLRLDTNPTGKEARLVQLKTAGGSKIQANLR
                     IRSLSLTNQIVALIFTPPASQAETEIKEGSFTFVESLLRVQAQDSTSAALSAAAEIVH
                     GALHAQAAAAYLLRKAENQLMRVRLNTDLQSAQFPEKLPLASAPALQPLVWKSNQKPS
                     SILEELALVKGFHYLLVLPMVSRNEMLGQIIAAGFGSPPDEENLRQLALLAAGTAGTL
                     HHLERVENAQRTVQRTRQVVQLEHAISDNMEEGLIILTPDLCVAEMNPSAEMMLGYAS
                     SEVFLQKADMVLIGNETLSALYKSAQQGISTKAGSNLRLNTRTGKDFLAQVLCIPVMT
                     DGKLSSIVLILHDLSQSEQIRVHTQHLEQRAYLGEVSAAFAHEAKNPINSIMTGLQYI
                     GMTMKSSDPHYDLVSRLQTDCLRLTHLMDSTLTFSKPMEYHFAPVDMAEMLPSILERW
                     APRMTHLNIRYNFDANPAHPLVKADYRALEQVFVNLVSNAVQAMEKTGGTLNIRVVSA
                     ADHLVPAQYDVIVADSGPGIPDDIKEYIFEPFVTTNASGTGLGLAISKQIVSAHKGTI
                     NLESYPGGTMFHVLLPKAE"
     gene            complement(4368..5780)
                     /locus_tag="PKL60_06935"
     CDS             complement(4368..5780)
                     /locus_tag="PKL60_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95904.1"
                     /translation="MLQTHYQQPLTAPSAHLAQTMTLLSLNSGELLQKVESELSTNPA
                     LELVEERRCPMCKRLLPATGACPICSQPTNLLSDEPIVFVSAPEDFYSSRTLSADEAP
                     EEPFASEMVSLPEYVLRQIAAELAVEDRSLAAYLLSHLDEDGFLTTTILDVARYHHRL
                     PEDIKALIERLKRCDPIGVCSTNPKEALLVQLEVLSESVEIPPFTREIVSDHLPKLSQ
                     HHYAEIARALGTSNIKVQTAAAFISENLNPFPARANWGDLQSPAASGVNVYHRPDILI
                     YHLNENPNNPLVVEIIHPIRGTLRVNPLFKSSVKQVEGEKLEEWKEDIEKASLLVKCI
                     QQRSNALRLLLEKLVVLQKDYVLSGEKEMQPLTRAEIAEILNVHESTISRAVAGKSVQ
                     LPNRRIVPLSIFFDRSLSQRTLIKEMIASEKRPLSDSEIQENLKAHGVNIARRTVAKY
                     RSMEGILPAHLRHNHSAATG"
     gene            5995..7170
                     /locus_tag="PKL60_06940"
     CDS             5995..7170
                     /locus_tag="PKL60_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW95905.1"
                     /translation="MKIRPAHTVENIKPYFFASLNKTLAELKARGVDIIRLDMGSPDL
                     PPAPFIIERLIESARDPKKHGYAPMGGSNEFLKAVTDYYKTRFEVSLNPKTEALALLG
                     SKEGIFNINHTLLDPGDLVLMPDPYYPVYLAGAQLADSRVYFMPLLKENEFLPDLKAI
                     PTEIAREAKMMWLNYPNNPTGAVAGCDFFAEAVKFARDNEIVIVHDAPYTDLTFDGYR
                     AHSILEVPGAKDVAIEFNSLSKTYNMAGWRIGMAVGNAEVIRLLASYKSQIDNSIFTP
                     IMDAGALALTGDQSWLVERNLIYQERRDVIYDGLVEAGFEVEKPKAALYLWARLPGKF
                     GDPMQFCADVLNETGVSLTPGVIYGPSGKDYIRISIITPTPQIKDAMKRLVDWVKAN"
     gene            7176..>7822
                     /locus_tag="PKL60_06945"
     CDS             7176..>7822
                     /locus_tag="PKL60_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNW95906.1"
                     /translation="MYTTRNDHIVQPEPETAIMVGAQVGAESAQGLSLEDSLAELELL
                     ADTAGIAVLATLTQNLDTINPRTFIGSGKVEELKLLAEELGVGVVLFDEELSPRHQRE
                     LEELLGKNLKLIDRSALILDIFSQHARTREGMLQVELAQLEYRLPRLTHAWTHLARQA
                     GGGGGRTGGAGGVGLRGPGETQLEVDRREIKAKIARLREELEKVREHRLRHREHRR"
BASE COUNT         1642 a         2076 c         2340 g         1764 t
ORIGIN      
        1 cggttgaccc gcggcttgtt tcaggaaggc gattttgcgc aattcgatta ccttctcaaa
       61 cgaaggcgct ttgaagtttt ctttttcggc gggcggctct tgcgggaagg ctgccagaaa
      121 actagctgcc aggcgcccga gaaattccgg gctttccacc gctttcagct gttggaggca
      181 ttcgcgctga aagtccaggg aagaggtacc gaacaagtcg atcaggagtt ggaagcggtc
      241 attctcaagc gtgggcgtgc agtccaccat atgcaggagc agcgcacaga cttttccggt
      301 ctgcgccagc ggggttttgg cggccagatc ctgcgcaagc tgtgggagct gcggcagaag
      361 cgcgggcaaa caggcaaacg cggagcgcca aaggtcgaga ttgccgcgcg gggcgctcag
      421 gaagctggaa acgtcctccc acccattgtt gagcgcgtgg taattccgca gcgcgagcgc
      481 tagcagtgcg gcttcttcca gtgtgctggg ttccaggccg gtcaatcgaa tggtatcgag
      541 cgtttttgtg gcttttgtgt tgagcgcggt gggaatttta acttcctgat ccagcaggtt
      601 ttgggctgcc agcttggggc tgagggtaaa caccgccagc agacctggct gccacagctt
      661 ggggtcctct ccggaaaatt cctcgaaagc gttcaacagc ccctcatcag tcagcgcttt
      721 ccataccagc ggctcagcgc ggaacgcact gatcaccgcc ttagccaact ccgaatcgaa
      781 acgggctttt accaggttaa gaaaacgcgc gatgctcatg cttccatcct tttccgggaa
      841 acgaggatca ggggacgatg ccgatgaagg atttgataaa gaaatacgcc gccgccagga
      901 gtaccaggct caggaagacg aaaaagatgc cgatgccggt gttggaacgg gagagatcgt
      961 tcagcgaact gatgagcagg gaagcgttcc ctaccagccc ggacacgccg tcggtgatgc
     1021 ctttttcagc caattttgtg gtttggttgg caatcgtctg gatgtttttt cccatcgcct
     1081 gcctggcgag aatgacaatg ctaagaataa gacaaagtac tcccaggcca aacaggatgt
     1141 aggaaatgag ctgtacgacc tgatttgttg tagaaatcat agaagcctcc tgacaagatt
     1201 acttgtaaga ggcttcgttg caattagcgt gccagaacaa atcatgtgcc gggttccacg
     1261 gcgctcagta catcggacga gaggtggttc aagtctatga cggggtcgtc gcagaagatt
     1321 accgcgcgtt ccatcacatt gcttagctcg cggatgttgc ccggccagga gtaatccatg
     1381 agggctttca tcgcgcgggg ggtcacatcc gtgatttgac tgcctgaacg cagggtgaat
     1441 ttgcgtaaaa agtaaccgac taaatccggg atatcctgct tgcgggcgga aaggggagga
     1501 agatccaggt cggagacctt taggcggtag tacaggtctt cgcggaagct tccgttggcg
     1561 atcatttcct tcaggttgcg gttcgaagct gccaaaatct ggacgtccac gttaatcagg
     1621 atgttgccgc ccagacgcct gaaagagcgc tcttcaatcg cgcgcagcag ttttgcctgc
     1681 aaatccatcg gcatggacgc aatttcgtcc aggaataata tgccgtcgtc cgccacttcc
     1741 atcaggccga cttttttgtg ggtggcgccc gtgaaagcgc cggcttcgtg cccgaacagc
     1801 tcggattcga acatagtgct ggggatagcc gggcagttga tatccacgaa gggcttgccg
     1861 gaacgcacgc caatcttgtg gatataatgc gccagcactt ctttgccggt gccggttggg
     1921 ccggtaatca acgccgaaat accgctttca gcagtccggc gggcatggtc gatgatttgc
     1981 ttcattgccg gggtttctcc gacgatgaat tcttcctggc cggcctgggc cgcacgcaaa
     2041 tggttcagtt ccctgtgcat cgcgacggtt tctccagccc gacgcaccga tttttccaat
     2101 tccaacaggt ccagcggttt ttcgatgaag tcctgtgctc cgttcttcat cgcttccact
     2161 gccatgctaa tctcgccgtg ggcggtgatg atgatgattt gtgggcggtt gaccatgcgc
     2221 gcggtttcgg tcagcaggtt ggggccgtag ccatcgggca ggcgcacgtc caggatgatg
     2281 atatccgcct ggcctttatt gatgaactgg cgggcttccc ccagggtgcc agctgagagg
     2341 acttcgtagc ccttggaacg taaaaactca gaaaccacca atctggcggt ttcctcatca
     2401 tccactacaa gaacggttgc tgtcatctca ccttattccg ctttcgggag gagcacatgg
     2461 aacattgttc cgcccgggta gctttccaga tttattgtgc ctttgtgcgc gctgacgatc
     2521 tgcttgctaa ttgccaggcc aaggccggtt ccactggcat tcgtggtaac aaacggctca
     2581 aagatatatt ccttgatatc atccgggatc ccgggtccgg aatctgccac gattacgtca
     2641 tactgcgctg gtaccaggtg atctgctgcg cttaccacac ggatattcaa agtgccgcct
     2701 gttttttcca tcgcctgcac tgcgttgctg accagattaa caaaaacctg ttcaagcgcg
     2761 cgataatccg cttttaccag ggggtgcgcg gggttcgcgt caaaattata cctgatattc
     2821 aggtgtgtca tgcgcggcgc ccagcgctcc aggatgctgg gcagcatttc tgccatgtcc
     2881 accggcgcaa aatgatattc catgggtttt gaaaaagtca gcgtcgagtc catcaagtgg
     2941 gtaaggcgca agcagtcggt ttgcaagcgc gaaacaaggt cgtaatgcgg gtcgctggat
     3001 ttcatggtca tcccgatgta ttgcaagccg gtcatgatgc tgttaatcgg attcttggct
     3061 tcatgcgcga atgcggcgga aacctcaccg agataggcgc gctgttccaa gtgttgggtg
     3121 tgcacccgga tttgctcgct ttgactgaga tcgtgcagaa tcagcactat actgctgagt
     3181 tttccgtcgg tcatcaccgg aatgcacagt acttgcgcca gaaaatcttt accggtgcgc
     3241 gtattgagcc tgagattgct gcccgctttg gtagagatgc cctgctgggc ggatttgtac
     3301 agcgcggaga gcgtttcgtt gccaattaag accatgtctg ccttctgcaa aaagacttcg
     3361 ctgctggcgt agcccagcat catttccgcg ctggggttca tttcagcgac gcacaggtcc
     3421 ggcgtgagga taatcaaacc ctcttccatg ttatcgctga tggcgtgttc caattggacg
     3481 acctgcctgg tgcgctggac ggtgcgctgg gcgttttcca ctcgctccag gtggtggagc
     3541 gtgccggcgg tgccggctgc caataaggca agctggcgca ggttttcttc atccggcggg
     3601 ctgccaaacc ccgcggcgat tatctgaccc aacatctcgt tccggcttac catgggcaga
     3661 actaacaggt aatggaagcc tttaaccaaa gcgagctctt ccaggatgga agacggcttt
     3721 tggttcgatt tccagacgag cggctgtagg gcgggggcgc ttgccagcgg cagcttttcc
     3781 gggaactgcg cggactgcag gtctgtgttc aggcgcacgc gcatgagctg gttctcggct
     3841 ttgcgcagca ggtaggctgc ggcagcctgg gcatgcagtg cgccgtgcac aatttctgcg
     3901 gccgcgctga gcgctgcaga ggtagagtcc tgcgcctgga cgcgcaaaag ggattcgaca
     3961 aaggtaaagc tgccctcctt gatttcggtt tccgcctgag aagccggcgg cgtgaagatc
     4021 agcgccacga tctgattggt caggctgagc gagcggatgc gcaggttcgc ctgaatcttg
     4081 ctgccgccgg cggttttcaa ttggactaag cgggcttcct tgccggtcgg gtttgtatcc
     4141 agcctgagcg aaatcaaggc gctgagggat ttccctgtca gcgccagcgg ttcaaacgag
     4201 cttaggtctt gcagcttctg attgaccgag atgattacgt cgctgctgcg gttgtaggcc
     4261 agcgcggcag aagggagcag ttccagaatc agcgcaaaat caagcgcctg caggtcatct
     4321 tccgcagggt cgaaaggcag ctggggagaa atcgggttcg ccaagtgtca tcctgtcgcg
     4381 gctgaatgat tatgccgcag atgagccggc aggatgcctt ccatcgagcg gtactttgcc
     4441 accgtccggc gggcaatatt cacgccgtgc gctttcaggt tttcctgaat ttcagaatcg
     4501 ctgagtgggc gcttttctga ggcaatcatt tccttgatga gcgtgcgctg ggagaggcta
     4561 cggtcaaaga atatcgaaag cggaacgatg cgcctgttcg gaagctgaac ggacttgccc
     4621 gcgacggcgc gcgagatggt ggattcgtgc acattcagaa tttcggcaat ctccgcgcgg
     4681 gtaaggggct gcatttcctt ttcgccggag agcacgtaat ctttctgcaa taccaccagc
     4741 ttttcgagca gcaaacgcaa ggcgttggag cgctgttgga tgcatttgac caataatgag
     4801 gctttttcga tatcttcctt ccactcctcg agcttttcgc cttccacctg tttcacggag
     4861 gacttgaaga gtggattcac gcgcaaggtg ccgcggattg ggtggattat ttcgacgacc
     4921 aacgggttgt tcgggttttc attcaggtgg tagatcagaa tgtccgggcg gtggtaaacg
     4981 ttcacgccgg atgcggcagg ggattgcagg tctccccaat tcgcgcgcgc agggaacggg
     5041 ttgagatttt cgctgatgaa tgccgctgct gtctggactt tgatgtttga ggtcccgagc
     5101 gcgcgggcaa tttcggcgta atgatgctgg ctcagttttg ggagatggtc ggagacgatt
     5161 tcgcgcgtga agggcggaat ttccacagat tcggacagca cttccagttg gaccaggaga
     5221 gcttctttcg ggttcgtcga acagacgcca attggatcgc agcgcttgag ccgctcaatc
     5281 agggctttaa tatcttcggg caggcggtgg tggtagcggg ctacgtccag gatggttgtg
     5341 gtcaagaaac cgtcttcatc caggtggctg agcagatagg ccgccagcga gcggtcctcc
     5401 actgccagct cggctgcgat ttgccgcagg acgtactctg gcaggctgac catctccgaa
     5461 gcgaaaggct cttccggcgc ttcatcagcg ctcagcgtgc gggaagagta gaaatcctcc
     5521 ggagcggaga cgaacacaat cggttcatcc gagaggagat tggtcggctg actgcaaatc
     5581 gggcaggcgc cggtggccgg gaggagacgc ttgcacatcg ggcagcggcg ctcctcaact
     5641 aactcgagcg cgggattggt cgataattcg gactcgacct tctgcaggag ctctcctgaa
     5701 ttcaagctga gcagcgtcat ggtttgtgcc agatgggcag agggggcggt gagcggctgt
     5761 tggtaatggg tctgaagcac gatattctcc tctccaggaa cagtataatc ttcaacctgg
     5821 aaagttgcaa gaagtgtacc aaacgggttt actggttttc ggaagctttt atctggcgcg
     5881 gcctgagctc aggcttcccg ggactgctta cttgccgccg gatggcagga aggcctgccg
     5941 ctttctttcc gccaaggtta taatggaaag aaaaaaggag ctttcttacc cgaaatgaag
     6001 attcgcccgg cacacaccgt cgaaaacatc aaaccatact tttttgcttc gctgaacaag
     6061 acccttgctg agcttaaggc caggggcgtc gatatcatcc gtctggatat gggttcgcct
     6121 gacctgccgc ctgctccctt tattattgaa cgcctgattg aatcagcgcg ggatccgaaa
     6181 aaacatggct atgctccgat gggcggttca aacgaattcc tgaaggcagt aactgactac
     6241 tataaaaccc gattcgaagt ctcactgaac ccgaaaaccg aagcgctggc gctgctcggt
     6301 tcaaaagagg gcatttttaa cattaatcac actctgttgg accccggcga tctcgtgctc
     6361 atgccggatc cttactatcc ggtttacctt gccggggcgc agttggcgga cagccgcgtc
     6421 tactttatgc cgctgctaaa agaaaacgag ttcctgcctg acctgaaggc catccctaca
     6481 gagattgccc gggaagcgaa gatgatgtgg ctgaattacc cgaacaatcc caccggcgcg
     6541 gtggcgggct gcgatttctt tgctgaggcg gtcaagttcg cgcgggataa cgaaattgtt
     6601 atcgtgcacg acgcgcccta taccgacctg acatttgatg gataccgggc gcattccatc
     6661 ctggaagtcc ccggcgcgaa agacgtcgcg attgagttca attcgctctc gaaaacctat
     6721 aacatggccg gctggcgcat cggcatggcg gtgggcaacg cggaagttat ccgtctgctg
     6781 gcatcttaca agagccaaat cgataactcc atctttaccc ctatcatgga cgcgggcgcg
     6841 ctcgcgttga ctggcgacca atcctggctg gtggagcgca acctgattta ccaggaacgc
     6901 cgcgatgtga tttacgatgg gctggtggaa gccggctttg aggtggaaaa accgaaggcc
     6961 gcgctgtatc tgtgggcccg gctgcccggg aaattcgggg acccaatgca attttgcgcg
     7021 gacgtcctga acgaaactgg cgtgagcctg accccgggcg tcatttacgg tcccagcggc
     7081 aaggattaca tccgcatttc cattatcact ccgaccccgc agataaaaga cgcaatgaag
     7141 cgcctggtcg attgggtgaa agccaactga gggctatgta cacaacccgg aacgaccata
     7201 tcgttcaacc cgaacccgaa actgccatca tggtcggcgc gcaagttggt gcggagtccg
     7261 cccaaggctt gagcctggag gattccctgg cggaactgga actgctggct gatacggccg
     7321 gcattgcagt gctggcgacg ctgacccaga accttgatac catcaatccg cgcaccttca
     7381 ttggcagcgg caaggtggag gagctgaaac tgctggcgga agaattgggc gtgggggtgg
     7441 ttttgtttga tgaagaactc tccccccgcc atcagcggga gctggaagag ctgctgggca
     7501 aaaacctgaa gcttatcgac cgtagcgcgc tgattcttga cattttctcc caacacgcgc
     7561 gcaccaggga aggcatgctg caggtggaac tggcgcagtt ggaataccgg ctgccccggc
     7621 ttacccacgc ctggacgcat ctggcgcgcc aggcgggcgg cggcggcggg cgcaccggcg
     7681 gcgctggagg ggtcggctta cgcggcccag gcgaaacgca attggaagtg gaccgccgcg
     7741 aaattaaggc caaaatcgcc cggctgcgcg aagagctgga aaaagtgcgc gaacaccgtc
     7801 tgcgccaccg cgaacaccgg cg
//
LOCUS       DAOQAD010000085         7736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_11355_length_7736_cov_5.676605, whole genome shotgun sequence.
ACCESSION   DAOQAD010000085 DAOQAD010000000
VERSION     DAOQAD010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11355_length_7736_cov_5.676605"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKL60_06950"
     CDS             <1..190
                     /locus_tag="PKL60_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95907.1"
                     /translation="VEKRFTLHLAVPTPTPTPTEMPTETPTEIPTVTPTPTPPLETPE
                     PPPTPTPGETLPPSDENP"
     gene            complement(215..2464)
                     /locus_tag="PKL60_06955"
     CDS             complement(215..2464)
                     /locus_tag="PKL60_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95908.1"
                     /translation="MSTTPRFDPLQAPFIAGSDRPQTGLPLDRFLPPYYPGMVSNWVN
                     SRAEPGAWILDPFGNDPFAALELARSGYRVLVAANNPIAAFILEVMASAPGVEEIGDA
                     VLALADLRMSEGARLEDYLIAFYQLPCPECGGLAEVTSFVWGEEGPEPVSMQWSCPRC
                     RKNGELPGSAEAYQRLKALPSYTLHRAKALELTASIDDQLRPVMEEVLRFYSPRALIL
                     LQILLNKINHPGFSERQRKLLKALFLTTADQMNQLWTYPLGRNRPRQLIRPPAWQESN
                     LWQAFLRSRSVWQTNQAPVTLRTWPDLPPQKGGISLFRGRLRELDPQPDPRLMSLVYA
                     SLPRRNQAYWNLSGLWAGWLWGKEGPTPLRHSLLRQRYDWTWHSQALVKVLSKLPMLV
                     QKQTPILLQIGELDGLFLIAGILAGQESGLRLLTCASDGEQDTLQCVWAIGANAAGLN
                     AKNLRLAAKEAAQAHLAERGEPASYLSLLTQIVVSTHQQGLLGAQAGKTLNELQEELE
                     ACLADTDAFVRFNPGIAPDTGQYWLRQPVIDHAPLAERLETALLEHLRANPSPSASAV
                     RRSANLSCPGLLTPEGELVDAVMSAYADRVPGSQPGWQLRVQEELQTREKDLSEIDSI
                     LRILAARLDYSIQSSKEALHWVGRTGIVAHSFFPVLTTCVTPLLHLFSRMPGQKYFLI
                     PASRSNLVSYRLKRDPNFQLLCGESWHFVKFRQIRNLQLDALLTRESFAQRVLEDPPE
                     FQRAQLSLF"
     gene            complement(2486..4093)
                     /locus_tag="PKL60_06960"
     CDS             complement(2486..4093)
                     /locus_tag="PKL60_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNW95909.1"
                     /translation="MSAQRTWWRDGVIYQIYPRSFQDTNADGLGDLQGIIQRLDYLKE
                     LGIDGIWLSPINPSPDVDFGYDVSDYHGIDPKFGTLQDFDRLLQEAHQRDIHIILDLV
                     LNHTSDQHQWFQESLKSKDNPYHDWYIWRDPAPDGDYPNNWLAIFGGPGWEYNSELNQ
                     YYYHTFCPEQPDLNFRNPDVRQAVLEVFRFWLERGVDGFRLDVFNAYYKHRELRDNPR
                     RKGFYLRPYDRFEHVNDCSQPEMFPLLQDIRRLVDQYPDRYVVGETFLASPVQARMYI
                     APDLLHAGFDYAYARSTWSASSFGNAIAYWDALHGEEDWPNYFLNNHDTPRSAARYVT
                     GEEDARLKLLACMHLTVRGTPYLYYGEEIGMRDTRYKRKEIQDPVGKRKWLSVQGRDG
                     CRSPMQWDASMNAGFSPARPWLRVNPDYISRNVEAQQQEPQSLLSFYRALIHLRRENP
                     VLHAGSLELLPLENESVLVYRRSTPSRQALVALNFSNRPQQITLPALEIENQTWQLLF
                     GGNGPAAPELDSGSLCLPPYGTAILLS"
     gene            4676..6586
                     /gene="nusA"
                     /locus_tag="PKL60_06965"
     CDS             4676..6586
                     /gene="nusA"
                     /locus_tag="PKL60_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNW95910.1"
                     /translation="MKSEFALAFNEVLEEKQLSREIIMGALETAMVAAYRKAVGASAA
                     QSVSVDIDMDKGSVKIFAEKEVADSIIDSRTEVLLDEARKVDPNAQIGDLVMVESTPE
                     DFGRIAASAARQAIQQKIRDAEKSQQFEFFSRQVGEIISGVLQAVNRVQATIGLDLKA
                     EGTMLRKDMIPGERFRLHERIRAYVYEVKDTPKGLQIMLSHTHRNFLRRLLENEVPEI
                     YHGIVEIRSIAREPGQRAKVAVSATQMGIDPVGACVGQKGVRIQAIVNELHGEKIDVI
                     EWKSDPAAYIEKAISPAKPVTGVYLSLANSENKTATVVVPEDQLSLAIGKEGQNARLA
                     AKLTGWRIDILSVPEAASRALVKLRDDPSLKDLAVQEAETTARVAEILRKKEEGKAPT
                     AEEVTLMARFVDRVEKRGEADRKQEDAAAQAHLKALRASIDPRAFEMPILDVPLKEHM
                     LAILQGAGMESFGDLMLQVKLDPDKILSLNGIGPKAMEEIQTLVQTYQFPTLETVPEP
                     AAVETPAEPEAVPEAALEGGAEAAVSEAVVEAVVVEAPTVEAPEEPAVVEESGGEEKS
                     FEELFKLESLRREEKARDVVTEDEESGDLKQGKKGKGKKKKGYTVEYDPDKEEDIVRY
                     KHKNDGEWDEDW"
     gene            6619..6948
                     /locus_tag="PKL60_06970"
     CDS             6619..6948
                     /locus_tag="PKL60_06970"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNW95911.1"
                     /translation="MAAKSKRKPKHVPQRTCVGCREVMPKRNLIRLVRTEQGVLVDPT
                     GKLAGRGAYLHDVKSCWVKGLKGPLANALRTGLTEQDMVALSAYMDTLPTETEKSGVS
                     ADSESVR"
     gene            7038..>7736
                     /locus_tag="PKL60_06975"
     CDS             7038..>7736
                     /locus_tag="PKL60_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HNW95912.1"
                     /translation="MSNDGKKDVTLPYSLSVRDLAAQLNASPVQVIKILMSNGVMASI
                     NQTVDFDTAAVVATELGFTPQLQKEEVPVEETGEIPLWRRVIMDEDPSKLENRPPVIT
                     ILGHVDHGKTTLLDAVRHTNVAGGEAGGITQHIGAYQVTHKNRLITFLDTPGHAAFSS
                     MRARGAKGADIVILVVAADDGVQPQTKEAVNHAKAAQVPIVVALNKIDKPDANPDLVK
                     RQLADIGLQPDEWEG"
BASE COUNT         1736 a         2112 c         2398 g         1490 t
ORIGIN      
        1 ggtggaaaag aggttcacgc tccacctggc cgtcccgaca cccacaccca cgccgaccga
       61 aatgccgacc gaaacaccga ccgagattcc gaccgtgacg ccgacgccga ctcctccgct
      121 ggaaacgccc gaaccgcccc cgacgccgac gcccggggag acgctgccgc cttcggatga
      181 aaatccgtag gggtttcgaa gaacttttcc gggattagaa caaactgagt tgggctcgct
      241 ggaactccgg cgggtcttcg aggacgcgct gagcgaaact ctcgcgcgtg agcagcgcgt
      301 ccaactgtaa attacggatt tgacggaatt tcacgaaatg ccagctttct ccgcacagca
      361 gctggaagtt gggatcccgc ttcaggcggt aagagaccag gttggagcgg ctggcaggga
      421 tgaggaagta tttttgcccg ggcatgcggc tgaagaggtg cagcaggggg gtcacgcagg
      481 tcgtcagtac agggaagaag ctgtgcgcca cgatgcccgt cctgccaacc caatggagag
      541 cttccttact gctttgaatg ctgtaatcca gtcgggcagc cagaatccgc aggatgctgt
      601 ctatttcaga caggtctttc tcgcgggtct gcagttcctc ctgaacgcgc agctgccagc
      661 cgggttggct gcccggcacg cggtcggcgt aagctgacat gaccgcgtcc accagttcgc
      721 cctccggggt aagcaggccg gggcagctca ggttggctga ccgccgcacc gcggatgctg
      781 aagggctggg attggcgcgc aggtgctcga gcagggctgt ttccaggcgt tccgccaggg
      841 gcgcgtggtc aatcacgggc tgccgcagcc agtattggcc ggtatccggg gcgatgccgg
      901 ggttgaaacg cacaaaggcg tccgtatccg ccaggcaagc ttccaattct tcctggagtt
      961 cgttcagcgt tttcccggct tgggcgccaa gcaaaccctg ttggtgcgtt gagaccacaa
     1021 tttgggtgag caaggaaagg tagctggcag gttcgccgcg ttctgcaagg tgcgcctggg
     1081 cagcctcttt ggcagccagg cgcaggtttt ttgcgttcag cccggcggcg ttggcgccga
     1141 ttgcccagac acactgcaag gtgtcttgct caccgtcgga agcgcaggtg agcaggcgca
     1201 gcccggactc ctgccccgcc aggatgccgg caatcaagaa aagcccgtcc agctcgccga
     1261 tttggagcag gattggggtc tgtttttgca ccaacatggg aagtttgctc aggactttca
     1321 ccagcgcctg ggaatgccag gtccaatcgt agcgctggcg cagcaggctg tggcgcagcg
     1381 gcgtaggacc ttccttgccc cacagccagc ccgcccaaag accgctcagg ttccaataag
     1441 cctggttgcg gcggggtaag gaggcataca ccagggacat caggcgggga tcaggctgcg
     1501 ggtcgagctc gcgcaagcgc ccgcggaaga ggctgatgcc gcctttttgc gggggcaaat
     1561 ccggccaggt ccgcaaagtc acgggcgcct gattcgtctg ccaaactgag cgggagcgca
     1621 aaaaggcctg ccacaggttg ctctcctgcc aggcgggcgg acggatgagc tggcgcgggc
     1681 ggttgcgccc gagcgggtag gtccacagct gattcatctg gtcggcggtc gtcaggaaga
     1741 gcgctttgag cagctttcgc tggcgctccg aaaaacccgg atggtttatt ttgttaagga
     1801 gtatctgcag caggatcagc gcgcgcggag aataaaagcg cagcacctct tccatgacgg
     1861 gcctcagctg gtcgtcgatg ctggcagtca gttccaaagc tttggcgcgg tgcaaggtgt
     1921 acgagggcag cgccttcaga cgctggtagg cttccgcgct gccagggagt tcgccgttct
     1981 tgcggcagcg cgggcagctc cactgcatgc tgacaggttc ggggccttct tccccccaca
     2041 caaaactggt aacttccgcc agccccccgc actccgggca gggcagctga taaaaagcga
     2101 tcaggtaatc ctccaggcgg gccccctcgc tcatccgcag gtctgccagg gcaagcactg
     2161 catcgccgat ttcttcgacg ccgggcgcgg atgccatgac ttccagaatg aaggccgcga
     2221 tggggttgtt cgcggcgacc agcacgcggt agccggagcg ggctaattcc agcgcggcga
     2281 aggggtcatt gccaaagggg tccaaaatcc aggcgccggg ctccgcgcgg gaattaaccc
     2341 agttggaaac catacctgga tagtagggtg gtaagaatct gtccaggggg aggccggttt
     2401 gggggcggtc gctcccggcg atgaaggggg cttgcagcgg atcaaatcgt ggggttgtgc
     2461 tcacttgccg cctctccccg caagcctaac tgagcaggat tgcggtgccg tagggcggca
     2521 agcacaagct gccggaatcg agctccggcg cagcaggtcc attgccgcca aaaaggagct
     2581 gccaggtctg attttcaatt tccaacgcgg gcagagtgat ttgctgcgga cggttggaaa
     2641 agttgagcgc cacaagcgcc tgccgggatg gggtgctgcg ccgataaacc aacacggatt
     2701 cgttttcgag cggcagcaat tccaggctgc cagcgtgcag cacggggttc tcccgccgta
     2761 agtggatgag cgcgcggtag aaagagagca gcgactgcgg ttcctgctgc tgggcttcca
     2821 cgttcctgct gatgtaatcc ggattcaccc tcagccaggg tcgggcaggg ctgaagccgg
     2881 cattcatgct cgcgtcccac tgcatggggg aacggcagcc atcgcgcccc tgcacgctga
     2941 gccatttgcg cttcccgacc gggtcctgga tttctttgcg tttgtagcgc gtgtcccgca
     3001 tgccgatttc ttcgccgtag tagaggtagg gagtaccgcg tacggtcagg tgcatgcaag
     3061 ccagcagctt caggcgggcg tcctcctcgc cggtcacgta acgcgcggcg ctgcgggggg
     3121 tatcgtggtt gttcaggaag taattcggcc agtcctcctc gccgtgcagc gcgtcccaat
     3181 aggcgatggc gttgccaaat gaggaagcgc tccaggtgct gcgggcgtag gcgtagtcga
     3241 agccggcgtg cagcaggtcg ggggcaatgt acatgcgcgc ctgaacgggg ctggccagaa
     3301 atgtttcgcc gaccacgtag cggtccggat attggtccac caggcggcgg atatcctgaa
     3361 gcagcgggaa catctccggc tgcgagcagt cgttgacatg ttcgaagcgg tcgtaggggc
     3421 gcagataaaa accctttcgg cggggattat cacgcagttc gcggtgtttg tagtaggcat
     3481 tgaagacgtc caggcgaaaa ccgtccacgc cgcgttccag ccagaagcga aacacctcca
     3541 ggactgcctg gcgcacgtcc ggattgcgga agttcaggtc gggctgctcc gggcaaaagg
     3601 tatggtagta gtactggttc agttcgctgt tgtattccca gcccgggccg ccgaaaatcg
     3661 ccagccaatt attcgggtaa tccccgtcag gcgccgggtc gcgccagatg taccagtcgt
     3721 ggtatgggtt gtccttggat ttaagcgatt cctgaaacca ctggtgctgg tcggaggtat
     3781 ggttcagcac cagatccaga atgatatgaa tatcccgctg gtgcgcttcc tgaaggaggc
     3841 ggtcaaaatc ctgcaacgtg ccgaatttgg gatcaatccc gtgataatcg gacacatcat
     3901 agccgaagtc cacgtcgggg gagggattga tcggcgagag ccagatgccg tcgatgccca
     3961 gttccttgag atagtccagg cgctgaatga tgccttgcag atcgccaagc ccatctgcgt
     4021 tggtatcctg aaaagaacgg gggtaaatct gatagataac gccgtcccgc caccaagtac
     4081 gttgtgcact catgttatcc tccggagttc catgtacatt atacatgcaa taacgcgggc
     4141 tttgagagcc tgtaatgcaa aggctgaggg caagggcaat gaatcattgg aatttcagga
     4201 aaatttcgtt ttgacagtct caagctcaat aaggcgacac aggtacccga acgcggacct
     4261 ttccccagcg gcaagggttt ttttgtggcg cgatcaatcc gctggggaaa gggaaaaacg
     4321 tttgatggag ggcgcggatc tctaacaaat atcaactccc cgtcccagcg aaagtttatc
     4381 aggatcatag gataaatttc gtccgcgtgg aaagaaagta agcggggaaa agctgttcgt
     4441 ccgcttgttc gtcaaaatag gatggtccct ttgtaaaacc actggggttt ggtaagttct
     4501 ttcttgagct tttatcaacc ccttgagagc ttttcttgca tttaatcgaa aacatggtac
     4561 actatgtgca ttattcaatg cgatactttt ctggtctgaa aagtgggagc ttcccacttt
     4621 tctttttatc aggaaggtat gcccacgaaa gaacggaata ttggagtaag aagagatgaa
     4681 aagcgagttt gccttagcct tcaacgaggt tctggaagaa aagcagcttt cgcgcgaaat
     4741 tattatgggc gcgctcgaaa ctgcgatggt cgcggcgtat cgtaaagccg tcggcgcttc
     4801 cgctgcccaa agcgtttcag tagatatcga tatggacaaa gggtcggtaa aaatctttgc
     4861 tgaaaaagaa gtcgctgact cgattattga ctcgcgcact gaagtgcttc tggatgaagc
     4921 ccgcaaggtg gacccaaatg cgcagattgg cgacctggtg atggtggaat ccacgccgga
     4981 agacttcggc cgcattgccg cctctgccgc ccgtcaggca atccagcaga aaattcgcga
     5041 cgcggaaaag agccagcaat ttgagttctt cagccgccag gtcggcgaga ttatcagcgg
     5101 cgtgttgcag gcggttaacc gcgttcaggc cacgattggg cttgacctga aagccgaagg
     5161 cactatgctg cgtaaagaca tgatccccgg tgagcgtttc cgcttgcacg aacgcattcg
     5221 cgcgtacgtc tacgaagtga aagacacccc caagggtctg cagattatgc tttcgcacac
     5281 gcaccgcaat ttcctgcgcc gtttgctcga gaatgaagtg cctgaaattt atcacggcat
     5341 tgtggaaatc cgttctattg cccgcgaacc ggggcagcgc gccaaggtgg ctgtctccgc
     5401 gacccaaatg ggcatcgacc cggtcggcgc ttgcgttggt caaaaaggtg tgcgcattca
     5461 ggcgattgtc aacgagctgc acggtgagaa aattgacgtg atcgagtgga aaagcgaccc
     5521 ggcagcttat atcgaaaaag ccatcagccc ggccaaacca gtcaccggcg tgtatctgag
     5581 cctggctaat tccgaaaaca agaccgccac ggtcgttgtc ccggaagacc aactgagcct
     5641 ggccattggt aaagaagggc agaacgcgcg cctggcggca aaattgaccg gctggcggat
     5701 tgacatcctg agcgttcccg aggcggcttc acgcgcgctg gtaaaactgc gcgacgaccc
     5761 ctcgctcaag gatttggccg tgcaggaagc tgaaaccaca gcgcgcgttg cggaaatcct
     5821 caggaagaaa gaggaaggca aagcgccgac cgccgaggaa gtcacgctga tggctcgttt
     5881 cgtcgaccgc gtggaaaaac ggggcgaagc ggaccgaaaa caggaagacg ccgcggccca
     5941 ggcgcacctc aaggcgctgc gcgcctccat cgacccgcgc gctttcgaaa tgcccatcct
     6001 ggacgtgccc ttgaaggaac acatgctggc aatcctgcag ggagctggca tggaaagctt
     6061 tggcgacctg atgctgcagg tgaagctcga cccggacaaa atcctctcgc tcaacggcat
     6121 cggaccgaaa gctatggagg aaattcagac gctggtgcag acttaccagt tccccacact
     6181 cgagaccgtt cccgagcccg cggctgtgga aacccccgcc gaaccggaag cggtcccgga
     6241 ggctgccctc gaaggcggcg ctgaagcggc tgtgagcgaa gcagtggtgg aagctgtggt
     6301 tgtggaagcc ccgacggtcg aagcgcctga ggagccggca gttgtggaag aatccggcgg
     6361 cgaggaaaag agcttcgaag aactcttcaa gttggaatcc ctgcgccgcg aggaaaaagc
     6421 ccgcgacgtt gtgaccgagg acgaagaatc cggcgacctg aaacaaggta agaagggcaa
     6481 aggcaagaag aaaaagggtt atactgtgga atacgaccct gataaggaag aagacatcgt
     6541 ccgttataag cataaaaatg atggcgaatg ggacgaagat tggtaaacaa gaacgagaat
     6601 taacccaagc agatcaaggt ggcagctaaa agcaaacgca aaccaaagca tgtcccgcag
     6661 cgcacctgcg taggatgccg tgaggtaatg ccaaaacgta acctgattcg tttggtacgc
     6721 acggaacaag gtgtgttggt ggacccgacg ggtaagctgg ccggcagggg agcatatctt
     6781 cacgatgtga aatcctgctg ggtaaaagga ctgaaaggtc cgcttgccaa tgcgctgcgg
     6841 accgggctta ctgagcagga tatggtcgca ttgagcgcct atatggatac cctgccaacg
     6901 gagacagaaa aatcaggcgt ctccgctgac agcgaaagcg ttcgttaagg attatcctat
     6961 gtaacgcttt tccctgtcag gctcagaaaa ccagatccaa cggcgccagc gacctgaatt
     7021 aacaatggag gtggattatg agcaatgatg ggaaaaaaga cgttacactc ccctatagtt
     7081 taagtgtccg tgatttggcc gcgcagttaa atgccagccc ggtccaggtg attaaaatcc
     7141 tgatgagcaa cggtgtgatg gccagcatca accagaccgt ggactttgac acggccgcgg
     7201 tggttgccac agaattgggc ttcaccccac agctgcagaa ggaagaagtg ccggttgagg
     7261 aaaccggtga aattccgctc tggcggcgcg tcatcatgga tgaagacccc agcaagctgg
     7321 aaaaccgacc ccctgtcatt accatcctgg gccacgtgga ccacggcaag accaccctgt
     7381 tggatgcagt gcgtcacacg aatgtggccg gcggcgaagc cggcggcatt acccagcata
     7441 ttggcgcgta tcaggtgaca cacaagaacc gtttgattac cttcctggat acccccgggc
     7501 atgcggcgtt ttcgtccatg cgcgcgcgcg gcgcaaaggg cgcggatatc gtaatccttg
     7561 tggtggctgc cgatgacggc gttcagcccc agaccaaaga agcggttaac cacgccaaag
     7621 ccgcccaggt tccgattgtg gtcgcgttga acaaaatcga taaaccggat gccaaccccg
     7681 acctggttaa gcgccagctc gcggacatcg gtctgcagcc ggatgagtgg gaaggc
//
LOCUS       DAOQAD010000086         7723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_11384_length_7723_cov_5.574074, whole genome shotgun sequence.
ACCESSION   DAOQAD010000086 DAOQAD010000000
VERSION     DAOQAD010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11384_length_7723_cov_5.574074"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PKL60_06980"
     CDS             complement(<1..938)
                     /locus_tag="PKL60_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNW95913.1"
                     /translation="MAAPQKSTIDSRLESILFRVEKPGRYVGGEHNQVRKSWHSIETH
                     VALVFPDIYDIGQPNLGLAILYDVLNQQPDVLAERAYAPWVDMETQLRESGLPLFSLE
                     SKTPLNKFDILGFTLPYEQIYTNVLNILDLSGLPLLSSERGAEDPLVIAGGQACYNPE
                     PMADFIDAFAIGEGEDLILDITRAYQEWKRWHGPREELLKALARIPGVYVPGFYEVTY
                     LDDGSIAHVSPRMQGAALPVQKRIVAKLPPATTRFLVPNVETVHDRISVEIMRGCTRG
                     CRFCHAGMVNRPVRERAPDEILQAMQAGLSATGCEQ"
     gene            complement(938..1723)
                     /locus_tag="PKL60_06985"
     CDS             complement(938..1723)
                     /locus_tag="PKL60_06985"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNW95914.1"
                     /translation="MKIVRFETKYTPPAYGWIDEDKVGLIQGDPYGEFRRYDPEFSLS
                     QVKLLVPCLPSKILAVGRNYEEHIREMNQTPPEIPLLFLKPPSTLIRSGDPIVLPPQS
                     ERVEHEAELAVVIKKKSRWLRPEAVREAIFGYTIANDVTARDLQQRDGQWTRAKGFDT
                     FCPLGPWIETEFNPADALITCRVNEILRQMASTRDMLFNIEQLILYASSIMTLLPGDI
                     ILTGTPAGVGRLSPGDVVSIFIDGIGSLVNPVAAGKAYEGKDS"
     gene            1748..1821
                     /locus_tag="PKL60_06990"
     tRNA            1748..1821
                     /locus_tag="PKL60_06990"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1782..1784,aa:Val,seq:tac)
     gene            complement(1894..2709)
                     /locus_tag="PKL60_06995"
     CDS             complement(1894..2709)
                     /locus_tag="PKL60_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNW95915.1"
                     /translation="MTSLLPEKFTFTQSNLQAYVNCKYQFYLRYIEHFLWPAPATTDM
                     LSFERDRQAGSRFHQLVHQLLLGLPASDLTLSAENDPDPRVGHWFDLFKRTIFKDLSG
                     KLFPEYTLTANLEGLILSAKYDLLRQTGEVYTIYDWKSSRRKPSKMWLEAQMQSKVFP
                     LVLSLQPALIADSAERHLRMVYWEVTEPEQPVVLEYSRAKLNAYQSEISALVSRIKAG
                     APQDFQKTADVQRCRTCIYRSFCNRPSEPASLEEYLSSEYLALEEDTELLSEG"
     gene            complement(2690..4936)
                     /locus_tag="PKL60_07000"
     CDS             complement(2690..4936)
                     /locus_tag="PKL60_07000"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNW95916.1"
                     /translation="MPDQRLRDSQKRILSYRAGKMGISAVPGSGKTWTLSRLAVKLVA
                     EMELQPDQEILIVTFSNSAADVFANRISSLLLERGMLSSLGYRVRTLHGLANDIIRER
                     PELAGLPNEFQILSEADTDSILSSCTATWYRNNADILDDFLRDDLEPRKLDQIRQKDL
                     PDLVKQTASAFIKEAKDHQLTPTKLQQLLSASYLKTDPLMQMGLEIYRDYHSALQYRA
                     AVDFSDLIRLAYQCLQEDPSLTELLRYRWPYILEDEAQDSSLLQEKILRTIVGTDGNW
                     VRVGDPNQAIYQSFTTANPNLLKSFLKEAGVRSEPLPESGRSCLEIIELANFFNDWTR
                     TQHPVADAREALTLPLIEPTRPDDPQPNPSPLPPAVEVDHRQVSPDLELEFLAKSTRD
                     WLERYPDTTVAVLALLNKRVSEIIKALKEHNIPVTDALTAMPQNTRATAGLVSRLLSC
                     VIRPEDPAALALAYQASRREERADEESWMLVKHCSDLIKKCEHIEDYLYPTGSTDWLR
                     QLEAGGTSAQVLSELSSFRDVARKWHLAAHLQPDQFILASVMDLELDNLELASVHNMA
                     LLLRELQDLHPDWTLAALNAELLAIAKNERRIARLSAEGEGFDPEAYRGQVVVATLHK
                     AKGLEWDKVYISSANTYDFPTGKEEERQHGEKHFLRGRMNMQAELVAQLRALAEGIFV
                     DASNKSAQRADSVRDRLRLLYVGITRARRSLTISFNTGLDKKQSEALAVRAMRAYLER
                     KKNDQLTP"
     gene            complement(4929..7022)
                     /locus_tag="PKL60_07005"
     CDS             complement(4929..7022)
                     /locus_tag="PKL60_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95917.1"
                     /translation="MKLSSEQAQLVALPLEKSLFLRGTAGTGKTTAALHRLRRLLTER
                     ADENLLILLPQRVMGSSLRRGLEESAAYNPIRVNLTTMNSIARRMVGLFWPLIGSRAG
                     FARPYAPPQFLTLETAQYSMGEIVNPMLAAGAFSSVSLPKHRLFSQILDSVNKSALIG
                     FPLGEICARLSSAWTGSPAQLNVYQDVQTAASAFREFCLENNLLDYSLQVELFRHFVW
                     EEELCQRFLKSSYQHLVYDNIEEDPPYVHDIISEWLPGFQSALLIFDENAGYRRILGA
                     DPASALRLEQHTLRTAVFHTQQPTAKMQALKRAFQGKPRNKQPIKQADLMQALHLPEK
                     PARFFPEMLTQSAEALSSLIQAGTPAGQIAVLAPYLSNAMGFAIQQELSAAGIPSRLL
                     RPSTPLLENPVIQQLITLTQLAHPTWTPAPDPNRITSALSASISGLDLVRAQLIRSEL
                     YLTGGQDFALLPFSAASVQLSGRISDELVLRYDTLRLWLQKAAQDEPLDLFLSRLFGE
                     LMTQDGFGFYQNLSAAQATAVLMESFRKFRLSLGPAQLGGQNQVNRDFTRSILDGVIS
                     AQYLRDWDLEDDGRVLITPALTFLSSGRTVDYQVWLSVGSAGWYERLEQPLTQPYVLS
                     REWEPGRKWTTEEEKDVSLALLDSVLEGLLSRCHKGVILGMSEFGQSGQEEKGLLLMK
                     IQGLLRQAAKDAGNA"
     gene            7210..>7723
                     /locus_tag="PKL60_07010"
     CDS             7210..>7723
                     /locus_tag="PKL60_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNW95918.1"
                     /translation="MNSSLKPFFNPQGVAILGASSKPNKLSYGILENLLKYGYQGGIY
                     PVNPNAQEILGVKVYPELAQVPDPVDLAMIVLPVGMILDTMQECADRGIKAVIIITGG
                     FREVGGEGAEIERKTLEFAREHGMRVVGPNCVGTMDIRNGLNATFIKGMPSRGPIAFI
                     SQSGAICGGVV"
BASE COUNT         1623 a         2073 c         2268 g         1759 t
ORIGIN      
        1 atttgttcgc agcctgtagc ggaaagcccg gcctgcattg cctgcaggat ttcgtccggt
       61 gcgcgttcgc gtacaggccg gtttaccatg ccagcatggc aaaagcggca gccgcgcgta
      121 cagccgcgca taatttccac ggatatgcgg tcgtgtacgg tttccacgtt tgggaccaga
      181 aagcgcgtgg tggccggcgg gagtttggca acaatgcgtt tttgaactgg cagcgcggcg
      241 ccttgcatcc gcggggatac atgggcaatg ctgccgtcgt ccaaatatgt cacttcataa
      301 aagccgggga cgtacacgcc tggaatgcga gctaaagctt ttagcaattc ttcccgagga
      361 ccgtgccagc gcttccactc ctgataggct cgcgtgatgt ccaggatcag atcttcgcct
      421 tcaccgatcg cgaacgcgtc aatgaaatcg gccatcggtt ccgggttgta acaggcttgt
      481 ccgccggcga tcaccagggg atcttccgcg ccgcgctcgc tggagagcag gggaagaccg
      541 gagaggtcca gaatattgag cacattcgta tagatttgct catatgggag ggtaaaaccg
      601 aggatgtcga acttattcag aggcgttttg gattccaggg agaacaacgg cagtccgctt
      661 tcccgcagtt gggtttccat gtccacccag ggggcgtagg cgcgttccgc cagaacgtcc
      721 ggctgctggt tgaggacgtc gtagagaatt gccagaccga ggttaggctg accgatgtca
      781 tagatatccg gaaaaaccag cgcgacatgg gtctcgatgc tgtgccagga ttttcgcacc
      841 tgattgtgct caccgccaac gtagcggccg ggtttttcca cccgaaagag gatgctctcc
      901 aggcggctgt caatggtaga tttttgtggt gcggccatca ggagtccttt ccctcgtagg
      961 ctttgccggc cgcgaccgga tttaccaggc tgccgatgcc gtcgatgaag atggacacga
     1021 catccccggg gctcagcctg ccgacgccgg cgggcgtgcc cgtgaggatg atatctccgg
     1081 ggagcagggt catgatggag gatgcgtaca ggatgagctg ttcgatgttg aagagcatgt
     1141 cgcgcgtgga agccatctgg cggagaatct catttacccg gcaggtaatc agcgcgtcgg
     1201 ctgggttgaa ttccgtttca atccagggtc ctaatggaca aaaagtgtcg aaacctttgg
     1261 cacgggtcca ttggccgtcc cgctgctgca ggtcgcgagc ggtgacgtcg ttcgcaatgg
     1321 tgtagccaaa gattgcttcc cggacagctt ccgggcgcag ccagcgcgat tttttcttga
     1381 ttacgaccgc gagttccgct tcgtgttcca cgcgttccga ttgtggcggc aggacgattg
     1441 gatcgccgct gcggatgagc gtggaaggcg gtttgaggaa gagcagcggg atttccggcg
     1501 gggtttggtt catctcgcgg atatgttctt cgtagttgcg ccctacagca aggattttgg
     1561 acggcagaca agggaccagc aatttgacct gtgagaggga aaactccggg tcatagcggc
     1621 ggaattcacc gtaggggtcg ccctggatga ggccgacttt atcctcatcg atccagccgt
     1681 atgcgggcgg ggtatatttt gtttcaaagc gcacgatttt cattttttgt gtataatttc
     1741 ttttctcggg cggatagctc agttggttag agcgcatctt ttacacagat gaggtcgcag
     1801 gttcgagccc tgttctgccc atatttttgt ttaacctcgt tattatacca acaccgggca
     1861 agacctgccc gtaaagctgc tcaggcaccg aaattatccc tcactcagaa gttctgtgtc
     1921 ttcctccagc gcaagatatt cgctggaaag gtattcttcc aggctggcgg gctccgaagg
     1981 gcggttgcaa aaactgcggt aaatgcaagt gcggcagcgc tggacgtccg cggtcttttg
     2041 aaagtcttgc ggcgcgccgg cttttatcct ggaaacgagc gcactaatct cggattggta
     2101 tgcattcagc tttgcgcggc tgtattccag cacaacaggc tgttcaggct ctgtaacttc
     2161 ccaatacacc atacgcaaat gccgctcagc gctgtcggca attagcgcag gctgtaaact
     2221 caggaccaga ggaaacactt tgctctgcat ttgtgcttcc agccacattt tggagggttt
     2281 cctccgcgag cttttccagt catagatcgt gtagacctcg ccagtctgcc gcagaaggtc
     2341 gtacttggcg ctgaggatca atccctccag gttggctgtc agcgtgtatt cagggaagag
     2401 ctttccagag aggtctttga agattgtgcg cttaaacagg tcaaaccaat gtccgacgcg
     2461 agggtccgga tcattttccg cgctcaaggt caaatcgctg gcaggcaaac ccagcagcag
     2521 ctggtgcacc agctggtgga agcgcgaacc tgcctgtcgg tcgcgttcga aagagagcat
     2581 atccgttgta gctggagccg gccaaagaaa atgctcgata taccgcaagt agaactgata
     2641 tttgcagttc acatatgctt gcaggttgga ttgcgtgaag gtgaactttt cagggagtaa
     2701 gctggtcatt cttcttcctc tcaaggtatg cacgcatcgc tcgaactgcc agcgcttcgc
     2761 tttgcttctt atccagaccg gtgttgaatg agatggtcag ggaacgcctt gcgcgcgtaa
     2821 tgccgacata taacaaacgc agacggtcgc gcacgctatc cgcgcgctgc gcggatttat
     2881 tgctggcatc cacaaagata ccctccgcca acgcgcgcag ttgggcgacc aattccgcct
     2941 gcatattcat cctgccgcgc aagaagtgtt tctcgccgtg ctggcgttct tcttccttgc
     3001 ccgtcgggaa atcataggtg ttggcagagc tgatgtagac cttatcccat tccaacccct
     3061 tggctttgtg gagggtcgca acgacaacct gcccccggta agcctcggga tcaaagcctt
     3121 caccctcggc tgaaagccgt gcaattctgc gttcgttttt cgcgatagcg agaagttctg
     3181 cattcaacgc ggcgagcgtc cagtccggat ggagatcctg cagctcccgc agcaacagcg
     3241 ccatgttgtg tacggaagcc aattcaaggt tgtcgagctc caggtccatg acggatgcta
     3301 agatgaactg gtccggctgc aggtgcgcgg caagatgcca cttgcgggcg acgtcccgaa
     3361 agctactcag ctctgaaaga acttgcgctg aagtaccgcc tgcttcaagc tggcgcagcc
     3421 aatctgtgct tccggtcgga tagaggtaat cttcaatgtg ctcacacttt ttgatgaggt
     3481 ctgaacaatg cttcaccagc atccagctct cttcatccgc gcgctcttcc cgccgggatg
     3541 cctgataagc cagggcaagc gcggctgggt cctccgggcg aatgacgcag gaaagcaagc
     3601 ggctcacaag cccagccgta gcccgcgtgt tttgcggcat ggcagtcagc gcatcggtca
     3661 cggggatgtt gtgttccttg agcgctttga taatttcact cacgcgtttg ttcagcaagg
     3721 cgagtactgc cacagtcgtg tccgggtaac gctccagcca atcccgggta gatttggcga
     3781 ggaattccag ttccaggtcc ggagaaacct gacgatggtc cacttcaacc gcaggcggga
     3841 gcggactggg gtttggctgc gggtcatcag gcctggttgg ctcaatcaac ggaagggtta
     3901 atgcttcgcg cgcgtccgca accggatgct gcgtgcgggt ccagtcatta aaaaagttgg
     3961 cgagctcaat aatctccagg caggaacggc cggattccgg cagcggttcg ctgcgcacac
     4021 ccgcttcctt gaggaaggat ttcagcaggt tggggtttgc ggtggtaaag ctctggtaaa
     4081 tggcttgatt cgggtcgcca acccgcaccc agttgccatc cgtgccaaca attgtccgaa
     4141 ggattttctc ctgcagaagg ctcgaatcct gagcctcgtc ttccaggatg tacggccagc
     4201 ggtagcgcag taattcggtc aggctcggat cttcctgcag gcattggtat gccagccgga
     4261 tgaggtcgga aaagtcgaca gccgcgcggt actgcagcgc gctgtggtag tcgcggtaaa
     4321 tttccagtcc catctgcatt aacggatccg tcttgaggta agaagccgag agcagctgct
     4381 ggagcttagt tggggtgagc tgatgatctt tcgcctcctt gatgaacgcc gaagccgttt
     4441 gcttgaccaa atccggcaaa tccttttgcc tgatttggtc cagtttcctg ggctccaggt
     4501 catcgcgaag aaagtcatcc aagatatccg cgttgttcct ataccaggtc gcggtacagc
     4561 tgctcaggat cgaatcggtg tccgcttcgc ttaggatttg aaattcattt ggcaatcccg
     4621 ccagctctgg ccgctcccgg atgatatcgt ttgccaggcc gtgcaatgtg cgcacccggt
     4681 atcccaggct ggagagcatc ccgcgttcca gcagcaggct gctaatccgg ttggcgaaaa
     4741 catctgctgc agaattggaa aaagtaacaa tgaggatttc ctggtcgggc tggagctcca
     4801 tttccgccac aagcttcacc gccagacggg agagcgtcca ggtcttaccg ctgccgggaa
     4861 ccgcggagat tcccattttc ccggcacggt agctgaggat gcgtttctgg gagtcgcgca
     4921 gccgctggtc aggcattgcc ggcgtccttt gctgcctggc ggagcaagcc ttgaatcttc
     4981 atcaggagca agcctttttc ttcctgcccg ctctgaccaa attcgctcat tcccagaatg
     5041 acgcctttgt ggcagcggct gagtaagcct tcgagaacgc tgtccaggag cgcaaggctt
     5101 acgtctttct cttcttcggt tgtccatttc ctgccgggct cccattcccg gctgagcacg
     5161 tagggctgcg tgagaggctg ttcaaggcgc tcataccaac ccgcgctgcc gacgcttaac
     5221 caaacctggt aatccacggt tctgccggaa ctgagaaagg taagcgcggg cgtgattaat
     5281 acgcgtccgt catcctccag atcccagtca cgcaagtatt gcgcggaaat cacgccgtcg
     5341 agaatgctgc gggtaaaatc ccggttcacc tgattttgcc cgcccaattg agccgggccc
     5401 agtgaaagcc tgaatttccg gaatgactcc atcagaacag cggtggcttg tgccgcgctg
     5461 aggttctggt aaaagccaaa accatcctgt gtcatcagct caccgaacaa acggctgagg
     5521 aaaaggtcca aaggttcatc ctgcgcggct ttttgcagcc acagccgcag cgtgtcgtaa
     5581 cgcagcacaa gttcatccga aatccttccg ctgagctgga cggaagcggc tgaaaatgga
     5641 agcagcgcaa agtcttgccc acctgtcaga tataattcgg agcggataag ctgcgcgcgc
     5701 acgaggtcca ggccggagat actggcggat aacgcgctgg taatcctgtt cgggtccgga
     5761 gctggcgtcc aggtcgggtg ggcgagctgc gtcagcgtaa tcagctgctg gatgaccgga
     5821 ttttccagca atggagtgga aggccgaagc agccgtgagg gaatgccggc agcgctgagc
     5881 tcctgttgaa tggcaaagcc catcgcgttg gataggtaag gcgccagcac cgcaatctga
     5941 ccggctggtg tgcccgcctg tatcaagctg ctgagcgctt cagcgctttg tgttaacatt
     6001 tcagggaaaa aacgcgcggg tttctccggc agatgcaggg cttgcatcaa gtcggcctgt
     6061 tttatcggct gcttattcct cggctttccc tgaaaagcac gtttgagtgc ctgcatcttc
     6121 gccgttggct gctgcgtgtg aaagactgct gtccgcagag tgtgttgttc caggcgcagc
     6181 gctgaggcgg ggtcagcgcc aaggatgcgg cggtagcctg cgttctcgtc gaatatcagc
     6241 agcgcgctct gaaagcctgg caaccattcc gagatgatat cgtgcacgta aggcggatct
     6301 tcctcgatgt tgtcatagac caggtgttgg tagctgcttt tcaagaagcg ctggcaaagt
     6361 tcttcctccc agacaaaatg gcggaacagc tccacctgga gagagtaatc gagcaggttg
     6421 ttctccagac aaaattcgcg gaacgcgctg gcggcggtct ggacgtcctg ataaacattc
     6481 agctgcgccg gactgccggt ccaggcggaa gaaagccgcg cgcaaatttc cccgaggggg
     6541 aagccaatca gcgcggattt gtttacgctg tcgagaatct ggctgaaaag gcggtgtttt
     6601 ggaagagaaa cagagctgaa cgcgccggcg gcgagcatgg ggtttacgat ttcgcccatg
     6661 gaatactggg cagtttccag cgtcaggaac tgaggcggag catatggacg agcgaatcca
     6721 gcgcggctgc caattagcgg ccaaaacaat cctaccatgc ggcgcgcgat gctgttcatg
     6781 gtcgtcaggt taaccctgat gggattgtag gcggcgctct cttccaatcc acgccgaagg
     6841 ctgctgccca tgacgcgttg gggcaagagg atcagcaggt tttcatccgc gcgctcggtc
     6901 agaagccgcc tgaggcggtg caaggcggcc gtggtcttgc ctgtgccggc tgttcctcgc
     6961 aggaagaggc tcttttccaa tggaagcgcc accaattgcg cctgttcaga cgacagtttc
     7021 atcctatatc gactctccga attctccaga gaaatggaat gcgattctcc caagcgttct
     7081 atacacaatc ccaattataa ggcaggataa aacttgagag ttcgcagcga ttgaggaaat
     7141 gccctgggct tcaaaacggc ttggtataat tcaggcaagc tcaccacata ccggaaagga
     7201 aatctttcga tgaattcttc cctgaaaccg ttttttaacc ctcagggcgt cgcgattttg
     7261 ggcgcttcca gcaaaccgaa taaactcagt tacggcattc tggagaacct gctcaagtac
     7321 ggatatcaag gcggaatcta tccggtgaat cccaacgcgc aagaaatcct gggtgtcaag
     7381 gtctacccgg aactggcaca ggtgcctgac cctgtagatt tggcgatgat tgtattgcca
     7441 gtgggcatga ttctggatac gatgcaggaa tgcgccgacc gcgggattaa ggcggtcatc
     7501 atcatcacgg gcggtttccg tgaggtgggc ggcgaagggg cagaaattga acgaaaaacc
     7561 ctggaattcg cccgcgaaca tggtatgcgc gtcgttggtc ccaactgcgt gggtacaatg
     7621 gacatacgca acggcttgaa tgccaccttc atcaagggca tgccttcgcg cggaccgatt
     7681 gcgtttatct cccagtccgg tgcaatttgt ggtggggtgg tgg
//
LOCUS       DAOQAD010000087         7688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_11458_length_7688_cov_6.026726, whole genome shotgun sequence.
ACCESSION   DAOQAD010000087 DAOQAD010000000
VERSION     DAOQAD010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11458_length_7688_cov_6.026726"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1029
                     /locus_tag="PKL60_07015"
     CDS             190..1029
                     /locus_tag="PKL60_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HNW95919.1"
                     /translation="MIFHITTTTEWETALKTGGYTPQPFTRDGFIHCSDLYQVEEVAN
                     RFYRDLPELIILGIDPDLTGIPLVYENLEGKTMRFPHLYGSPLPVESVKAVISLIRDD
                     KGDWRLPPAMRRSKPPLMNELPMGLPGKLYRSVMPGSRMFDPEDQVMNLYAQNGIQTV
                     VVLNSELDMREHALRDPRERYAQAGLDQIHAPVPDFSAPSAGTWNAALQQVAALLRAG
                     TIVAVHCHAGIGRTGMFCACLAQEVLGLSPQESITWVRGCIPGAVETEFQKQFVLEYP
                     LTR"
     gene            complement(1215..2177)
                     /locus_tag="PKL60_07020"
     CDS             complement(1215..2177)
                     /locus_tag="PKL60_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95920.1"
                     /translation="MKNIWSSVVVLLAAAFMLGACMGSQPLSFEDVQTQAAIFAGATL
                     TRIALVTQAAPTNTNPAPPPDNTALPPSATPMPPTAEPATATALPPTATALPPTETRV
                     PTVGITYITQSATPRPVVYDPIRLSFAEGTTNISLESSAAYNSVRRFVFWAAKNQLVK
                     ISLISANKVVLGVSGANGAVLLPISEGKTSYQGYLKANGDWYIDAAANPQEVEFTVSL
                     EIPERLSFPAGAYGLTASGKVPANSTHEYLVWANKGQRLKVSVQPGDKAVLAITHVDG
                     TELLSSSAGAASFNDLLPKAGDYIVRVVNRTGSSLTFSLPMEIR"
     gene            2391..2978
                     /locus_tag="PKL60_07025"
     CDS             2391..2978
                     /locus_tag="PKL60_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNW95921.1"
                     /translation="MAKTLLLMRHAKSSWHDKDLPDYQRPLKKRGAEDAAEIAEVLRE
                     NELVPQIILSSPALRASQTAEIVAKVCNLEQKINYVNSFYMGEVREFVEQLQQLDDGL
                     ERVMIIAHNPGLEAFMQLLDGKIEALPTAALAYISLDLHKWSDITKDSTGELIGYWDP
                     ENLDKQAQESEMGKNKKDKKEKKEKKEKKEKKDKK"
     gene            complement(3121..3525)
                     /locus_tag="PKL60_07030"
     CDS             complement(3121..3525)
                     /locus_tag="PKL60_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95922.1"
                     /translation="MRQQLTPQWSIDLPEGFQRRREDAHIVFWTVGITIGVVVFSYSG
                     AKEQAALLANLRAKAQAEELEIIEEQEGALIRFGTLQVEAITPKHTRLALHAFTTAEN
                     GCAQTSFYLDNAADLQHALKIWQSVQFRPAEQ"
     gene            complement(3550..5775)
                     /locus_tag="PKL60_07035"
     CDS             complement(3550..5775)
                     /locus_tag="PKL60_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNW95923.1"
                     /translation="MAKRKLKRQLNLLQVIMLGTAGTIAAEIFVLTGHAAGIAGPKAV
                     LALVLGGVLTLSIALNYCELATTYPVTGGAMTYVGQGFGNGLLMFLVGSLDCLSSTFY
                     AALSAIGFAISLKVFFPVLPIIPVALAVIVVVAAMHVRGVTKVGNFQIVLGAFLLIVF
                     AFYIIRGLTDPNGFSPSTFSSGHVVFAGQNTFQIIARMLSAIALTYNAYVGFEVIADD
                     AEEITKPNRNIPLGILMSLGIATLVYSLVSLVTIGTIPFRQLAGSTTALTDAAARFWP
                     NGGVQIMAAAGIVATLTSVNSAMLSATREAFTLSRERVWPRVFSRLSRWRTPYTAIIL
                     IALISALVAAIGVVDFLSYISSAGYMFVLFWASLAMLRLRRDQPNIERPFKVPFYPLT
                     PYLAAASGVLIIAFADPKALLFLAAVLVGLSLIFWVARSYNTRAELQQKLELEEGGGR
                     ILVAAVHQDTAMSLTRLAARLVDQQEDTSICVFSVKKTPFNLSEEQVPAFVEEQKAMK
                     RQLLAVTAPIAQSRNVPLYVKSKAARHVEDAVLDELARHREISMVLLGYPKDESKVHT
                     PNNILKEIVMTAQRDVGVLRDRGLERGIRRILVPVGNGPNARLALRLARDLATPEPIP
                     VTALRLILHYDDAELVEDQQAQLHDIVELELGEVPPYVELKAETVSSVLEGILEESKR
                     GNYDLMIIGAAEEVLSPDQIFGTLNDSLLEEAECSILVVRQYQPTGTIWLHKQLKKIE
                     E"
     gene            5909..7171
                     /gene="glgC"
                     /locus_tag="PKL60_07040"
     CDS             5909..7171
                     /gene="glgC"
                     /locus_tag="PKL60_07040"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNW95924.1"
                     /translation="MSVLTLILAGGAGSRLSILGEKRAKPAVPFAGKYRIIDFPLSNA
                     VNSDLYRLAVLTQFRPHSLMQHLGIGEPWDLNRRPPNGLQIWQPFRGRSQQDWYRGTA
                     DALYQNRSFIQDANSDTLLILSGDHIYKQDYRDLLRFHQSKNADLTVAVMNVSPQDVS
                     RFGIMSVDSAQKITKFAEKPKKSDSTLASMGIYVFNTRFLLEQLELDAAQPNSAHDFG
                     KNIIPHIVEMGRAFAYPFVGYWVDVGTIQAYWETSMALLEEEPALDLYDTDWIIHTRS
                     EERPPVKIMPPGTLEKSMASNGCNICGTVINSVLSPGVIVEKGAVVRNSVIMNDTIIR
                     AGASVANCVLDKHIEVGPAAQLGYGKDSIPNQLEPSNLYTGITIVGKSAVIPAKAVIG
                     KNCRIDPDTGPSDYETLDIPSGGSILKK"
     gene            7182..>7688
                     /locus_tag="PKL60_07045"
     CDS             7182..>7688
                     /locus_tag="PKL60_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95925.1"
                     /translation="MSTDLQQTPAWQALEAHYAQVKDLHMRALFAQEPDRFERFSIRF
                     KDILFDYSKNRVTDETIRLLLNLAEDAQLGAQIEAMFSGEKINNTEGRAVLHTALRNQ
                     SDAPVMLDGADVMPEIRRVLAKMRRFSESVRSGEWRGFTGKPITDIVNIGIGGSDLGP
                     KMVCAALKP"
BASE COUNT         1714 a         2317 c         2079 g         1578 t
ORIGIN      
        1 ttcctgccat ccgctcagcg cttgcaccag tatatcacga caatcctgcc gcgcgatgtt
       61 aatgtttttg tatatgttaa tgttttgtat atgtcttgca gtcccttgcc tgaaaaaagg
      121 taaaataagg catctggatg cgggcggccg ccggcatcta tgaaaacatc acttaggaga
      181 aggggttcga tgatttttca catcacaacc acaacagaat gggaaaccgc cctcaaaacc
      241 ggcgggtaca cgccccagcc cttcactcgc gacggcttca tccattgctc cgacctttat
      301 caggtcgagg aagttgccaa ccgcttttat cgggatttgc cggaattgat catcctgggt
      361 attgaccccg acctgacggg catcccgctg gtctacgaaa acctggaagg caaaacgatg
      421 cgcttccccc acctctacgg cagcccactg cccgtggaat cggtcaaagc cgtgatttcg
      481 ctgatccgcg atgacaaagg cgattggcgc ctgccgcccg caatgcggcg ttcaaaaccg
      541 ccgctgatga acgaactgcc tatgggtctc cccggaaaac tctaccgttc ggtaatgccc
      601 ggcagccgca tgttcgaccc tgaagatcag gtgatgaacc tctatgcgca aaacggcatt
      661 cagacggtcg tggtgctcaa ctccgaactc gatatgcgcg agcacgccct gcgcgacccg
      721 cgcgagcgtt acgcccaggc cggtctggac caaattcacg cccccgtccc ggatttctcc
      781 gctccgtccg ccggcacctg gaacgccgcc ctgcagcaag tggcagcgct cttgcgcgcg
      841 ggtacgattg tcgccgtgca ctgccacgcc ggcatcgggc gcaccggcat gttctgcgcc
      901 tgcctcgccc aggaagtgct ggggctcagc ccgcaggaat ccatcacatg ggtgcgcggc
      961 tgcatccccg gcgcggttga gaccgaattt caaaagcagt ttgtgctgga gtaccccctg
     1021 acccgctgaa aatgaggctg tacaaaaaga agtggctttc ctttttggac agcccaaagt
     1081 gggagaacgt gcctggttta tccctcgttt tggtagcgta aatccgccaa agaccataaa
     1141 aacggacacg gagtgggaaa tcaaaaaggg ctgtccgact tttggaacag ccccttcaac
     1201 catgcctgtg tatcttatcg gatttccatc ggcagcgaga aggtcaagct gctgccagtg
     1261 cggttcacca cccggacgat atagtccccg gctttgggca gcagatcatt gaagctggct
     1321 gcccccgcgc tggaggaaag cagctccgtt ccatcgacgt gagtaatcgc gaggaccgct
     1381 ttatccccag gctgaaccga gactttcagg cgctgccctt tattcgccca caccaggtat
     1441 tcatgcgtgc tgtttgccgg cacctttcca gaagcggtca ggccgtacgc gcccgcgggg
     1501 aagctcagcc tttccggaat ttccagagat accgtgaact caacttcctg cggattggca
     1561 gccgcgtcga tgtaccagtc cccgttcgcc ttcaggtaac cctgatagct ggttttccct
     1621 tcggatatcg gcaacagcac cgcgccgttc gccccgctga cgcccagcac gactttattc
     1681 gcgctgatca gtgagatttt gaccagctgg ttcttcgccg cccagaacac aaagcggcgc
     1741 acggaattgt aggctgcgct gctttcaagg ctgatgttcg tcgtcccttc ggcaaagctc
     1801 aggcggattg ggtcgtacac caccggccga ggcgtagcgg attgcgtgat atacgtgatt
     1861 cccacggtcg ggacgcgcgt ttcggtcggc ggcagcgctg ttgctgtcgg cggcagcgcg
     1921 gtcgcggtcg ctggctcggc agtgggcggc atgggcgtag cgcttggggg tagcgctgta
     1981 ttatccggcg gaggggctgg attcgtgttg gtcggcgcgg cctgcgtcac caacgcgatg
     2041 cgcgtcagcg ttgccccggc aaagattgca gcctgggttt gcacgtcctc aaaactgagc
     2101 ggctgcgagc ccatgcaggc ccccagcata aaagccgccg ccagcagtac aacaacacta
     2161 ctccagatat ttttcatcct gcctcctgac acttccataa cctgacttcg ttgcaagaag
     2221 ctttccagtt ccccggaaaa agcctgcatt ggaatccagc cgtgctgtgc ccaaatgcgc
     2281 agatcgacac ggaaaaatgc tggaattctt tggacaatcc tgaattaatc gtaatcctta
     2341 ttcgcgttta tgttatcatt tgttttgaca aaccaagatc ggagcgaacg atggcaaaaa
     2401 ccttgttgct gatgagacac gcgaaatcaa gctggcacga taaagacctg cccgattacc
     2461 aacgcccgct caagaaacgc ggcgcggaag atgcggctga aatcgccgaa gttctgcgtg
     2521 agaacgaact ggtcccgcaa atcattttgt cctcccccgc gctgcgcgcc agccagaccg
     2581 ccgagattgt tgccaaggtt tgcaatcttg agcagaaaat caactatgtc aacagttttt
     2641 acatggggga agtgcgggaa tttgttgaac agctccaaca actggatgat gggctggaaa
     2701 gggtgatgat tatcgcccac aatccgggtc tggaagcctt tatgcagctg ctggacggca
     2761 agattgaagc cctccctact gcagcgctcg cttacatcag tcttgacctg cataaatggt
     2821 cggatattac gaaagacagc accggtgaac tgatcgggta ttgggatccg gaaaatctcg
     2881 acaagcaagc tcaggagagc gaaatgggca agaataagaa agataagaaa gaaaagaaag
     2941 agaagaaaga aaagaaagag aagaaggaca agaagtaagt acgtcctttc tatccggcaa
     3001 ttttcttttc cgcaagtttt gacaggcgct aatctgtcag gcagatcaat gcgccctgtt
     3061 tcagattaag aacttcccaa atcccctcat atccccccgc gcgaaaagcg tttatcctga
     3121 ctactgctct gccggcctga actggacgct ttgccagatt ttcagcgcat gctgtaagtc
     3181 cgcggcattg tccaggtaaa atgaagtttg cgcgcagccg ttttcagcgg tggtaaaggc
     3241 gtgcagcgcc agccgggtat gtttgggcgt gatagcttcc acctgcaggg ttccaaaacg
     3301 aatcagcgct ccctcctgct cctcgatgat ttccaattcc tccgcctggg ctttggcgcg
     3361 caggtttgcc aacagcgccg cctgctcctt tgcgccgctg tacgagaaca cgaccacacc
     3421 gatagtgatg cccacggtcc agaacacaat atgcgcgtct tcccggcggc gttgaaagcc
     3481 ttccggcagg tcgatgctcc attgaggggt tagctgctgt ctcatagagc aattctatca
     3541 gaccacgcct tattcctcaa ttttcttcag ctgtttgtgc agccagatgg tgccggtggg
     3601 ttggtactgg cgcacgacca agatggagca ctcagcctct tccagcaggc tgtcgttcag
     3661 cgtgccaaag atttggtccg ggctcaacac ttcttccgcc gcgccgataa tcatcaggtc
     3721 ataattgccg cgcttgcttt cctccagaat cccttccaat accgaagaaa cggtttccgc
     3781 cttcaattcc acgtaaggcg gcacttcacc cagctcaagc tccacgatgt catgcagctg
     3841 cgcctgctgg tcttccacca gctcggcgtc gtcatagtgc aggatcaggc gcaacgccgt
     3901 gaccggaatc ggttccgggg tagccagatc ccgcgccagg cgcagcgcca ggcgcgcgtt
     3961 agggccgttc cccaccggta ccaaaatccg ccggatcccg cgctccagcc cgcggtcccg
     4021 cagaactccc acgtcgcgct gggcggtcat gacgatttct ttcaaaatat tgttgggcgt
     4081 atggaccttg ctctcgtcct tggggtagcc caggagcacc atggaaattt cccggtgccg
     4141 ggctaactcg tcaagtacgg cgtcttcaac atgccgggcg gccttgctct tgacgtacag
     4201 cggcacattc cggctctggg cgatcggcgc ggtcaccgcc aacagctggc gcttcatcgc
     4261 tttttgctct tccacaaagg ccggcacttg ctcctctgac aggttaaaag gcgtcttttt
     4321 gacgctgaaa acgcagatgc tggtatcctc ctgctggtcc accaaccggg cggccaggcg
     4381 cgtcaggctc atcgcggtat cctggtgcac agccgccacc aaaatcctcc cgccgccttc
     4441 ctcgagttcc agtttttgct gcagttccgc ccgcgtattg taggagcgcg ccacccaaaa
     4501 gataagcgac aaacccacca gtaccgcagc caggaatagc agcgctttcg ggtcggcaaa
     4561 agcgatgatg agtacgcccg aagcagccgc gaggtacggt gtgagcgggt agaagggtac
     4621 cttaaaaggg cgctcgatat tgggttggtc acggcgcaag cgcagcatgg ccaggctggc
     4681 ccagaagagc acgaacatgt atcccgcgct ggaaatgtaa ctcaagaaat ccaccacccc
     4741 gatagcggct accagcgcgc tgatcagcgc gatcagaatg atggcggtat aaggcgtacg
     4801 ccagcggctg agccttgaaa acacgcgcgg ccacacgcgt tcacggctga gcgtgaaggc
     4861 ttcgcgcgtg gccgagagca tggcggaatt gacggaagtc agcgtagcta cgatgccggc
     4921 cgccgccatg atctggactc ctccgttcgg ccagaacctg gccgctgcgt cggtcagggc
     4981 ggtcgtagaa cccgccagct gccggaaagg tattgtgccg atggtaacca gcgagactag
     5041 cgagtaaacc agcgtggcaa tccccaggct cattaaaatg cccagcggta tgttgcgatt
     5101 gggcttggtg atctcttccg cgtcgtcggc gataacctcg aaaccgacat aggcgttgta
     5161 ggtcagcgcg atagccgaaa gcatacgtgc gatgatctga aaagtgtttt gcccggcaaa
     5221 taccacatgc ccggagctaa aggtcgacgg gctgaagccg ttcgggtcgg tcagcccacg
     5281 gatgatgtaa aacgcaaaga caatgagcag gaaagcaccc aggacaatct ggaagttccc
     5341 caccttcgtg acgccgcgga cgtgcatagc cgccacgacc acaattaccg cgagcgccac
     5401 cgggatgatt ggcaaaaccg ggaaaaagac cttcagcgat atcgcgaaac caatcgccga
     5461 aagcgccgcg taaaaagtgc tggacaggca gtccaatgaa ccgaccagga acataagcag
     5521 cccgttgcca aagccctgac cgacataggt catcgctccg ccggtcacgg gataggtggt
     5581 cgcgagttca cagtagttta gcgcgatgct gagcgtgagc acgccgccaa gaaccagtgc
     5641 cagcaccgcc ttcggccccg caataccagc cgcgtgcccg gtcagaacaa agatttccgc
     5701 cgcgatcgtt cccgcggtgc ccagcatgat cacctggagt aggtttaact gccgcttgag
     5761 ctttcttttt gccatcgcat ataccccgga acgtttgttg tgccttttat tgattttatc
     5821 ctgactttgt ttattgcgcc gtacccctga ggctgttatg attaacctgc tgcgattaac
     5881 ccagatttgt ctcaaccgga ggagcactat gtctgttctg accctcatcc ttgccggcgg
     5941 agccggcagc cgcctgagta ttctcggaga aaaacgcgcg aaaccagccg tacccttcgc
     6001 cggtaagtac cgcatcattg attttcccct ctccaacgcc gtcaattccg atctgtaccg
     6061 cctggccgtc ctgacccaat tccgcccgca ctccctgatg cagcatctcg gcatcggcga
     6121 accctgggac ctgaaccgcc gcccgcccaa cggtctccaa atctggcagc ctttccgcgg
     6181 gcgctcccag caggattggt accgcggcac agccgatgcc ctctaccaga accgcagttt
     6241 catccaggat gccaacagcg acaccctgct catcctctcc ggcgaccata tctacaaaca
     6301 agactaccgc gacctgctgc gcttccatca atccaaaaat gccgacttga ccgtggcggt
     6361 gatgaacgtc agtccgcagg atgtcagccg tttcggtatc atgagcgtgg attccgccca
     6421 gaaaatcaca aaattcgcag agaagcccaa gaagtccgac agcaccctgg cctccatggg
     6481 catctacgtc tttaacacca gattcctgct cgaacagctc gagctggacg ccgcccagcc
     6541 caattccgcc catgattttg gtaagaacat catcccccac atcgtggaaa tggggcgcgc
     6601 ctttgcctat ccctttgtcg gctactgggt ggacgtcggc accattcaag cctactggga
     6661 aacgagcatg gcgcttctgg aagaggaacc cgccctcgat ctatacgata cggattggat
     6721 tatccataca cgctccgagg agcgcccgcc cgtaaagatt atgccgcccg gaacgcttga
     6781 aaaaagcatg gcgtccaacg gctgcaatat ctgcggcacg gttatcaaca gcgtgctttc
     6841 tcccggcgtc atcgtggaaa aaggcgctgt cgtgcgtaac agcgtcatca tgaacgacac
     6901 catcatccgc gcgggcgctt cggtggccaa ttgcgtgctg gataagcaca ttgaagttgg
     6961 ccccgcggcc cagctcggtt acggcaaaga ttccatcccg aatcaattgg agccgagcaa
     7021 cctgtataca ggcatcacca ttgtcggcaa atccgccgtc atccccgcca aggccgttat
     7081 cggcaagaac tgccgcatcg acccggacac cgggccttcg gattacgaga cgctcgacat
     7141 ccccagtggt ggcagcatcc tgaagaaatg acgaggtgaa gatgagcact gacctgcagc
     7201 aaacacccgc ctggcaagcg ctggaagcac actatgcgca agtgaaagac ctgcacatgc
     7261 gcgcgctttt cgcgcaggaa cctgaccgct tcgagcgctt ttccatccgg ttcaaagaca
     7321 tcctctttga ctattccaag aaccgcgtta ccgatgagac catacgcctg cttctgaacc
     7381 tggcggagga cgcgcagctt ggcgcgcaaa tcgaagccat gttcagcggc gagaaaatca
     7441 acaataccga gggacgcgcc gtcctgcata ccgctctgcg caatcaatcc gatgctcccg
     7501 ttatgctgga cggcgcggac gttatgccgg aaatccggcg ggtgctggca aagatgcgcc
     7561 gcttcagcga aagcgttcgc tccggggaat ggcgcggctt taccggcaaa ccgattacgg
     7621 atatcgtcaa tatcggcatc ggaggttcgg acctgggacc caagatggtc tgcgcagcgc
     7681 tgaaaccc
//
LOCUS       DAOQAD010000088         7442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_12030_length_7442_cov_6.568837, whole genome shotgun sequence.
ACCESSION   DAOQAD010000088 DAOQAD010000000
VERSION     DAOQAD010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12030_length_7442_cov_6.568837"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKL60_07050"
     CDS             <1..327
                     /locus_tag="PKL60_07050"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. The beta-hairpin of the
                     Uvr-B subunit is inserted between the strands, where it
                     probes for the presence of a lesion; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HNW95926.1"
                     /translation="PEAYIPRRDLYIEKETDINEDIERLRLSATTSLMSRQDVIIVAS
                     VSAIYGLGDPEAYGKSVLTIQKGKIYRRNALLRQLVDIQYQRNDFELKPGVFRLRGDT
                     LEILPA"
     gene            341..1756
                     /locus_tag="PKL60_07055"
     CDS             341..1756
                     /locus_tag="PKL60_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNW95927.1"
                     /translation="MRISYFGDEVEAITRLNRLTGEVSAELDQVEIYPAKHYITEEHR
                     MEKTLADIEAELEERLKYFRSEGRVVEAQRLEQRTRFDLEMLREVGSCAGIENYSRHI
                     DQRAPGTPPWTLLDFMPSRYMLVLDESHMMVPQIRGMYNGDRSRKEILVEYGFRLPSA
                     LDNRPLKFPEFERMMGTTLYTSATPGPYEMGKAGQVVEQIIRPTGLLDPQISVRPSQG
                     QIDDLIGELRKRVEKGERALVTTLTKRMAEDMANYLMEMGFKVHYLHSEVETLDRVGI
                     LRDLRTGVFDVVVGINLLREGLDLPEVSLVAILDADKEGFLRSSTALIQNFGRAARHI
                     NGEVILYADKMTDAMKSAIDETNRRRARQEAYNIEHHISPVGITKAVRDITDQLASSA
                     GVEHTESRSARELAARMETREMEKMIREMEKQMRAAAAELQFERAAMLRDQVYELRAL
                     LAENSKLPPWKQQNLLAGRED"
     gene            complement(1760..2059)
                     /locus_tag="PKL60_07060"
     CDS             complement(1760..2059)
                     /locus_tag="PKL60_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNW95928.1"
                     /translation="MDANPEANQRLHVLVEGHVQGVGFRYFTLEQARRLGLSGWVRNL
                     PGGAVEAEAEGPREDLLAFLEALAEGPSGSVVRRIRPEWLPANGGDSSFRVRIYE"
     gene            complement(2086..3219)
                     /locus_tag="PKL60_07065"
     CDS             complement(2086..3219)
                     /locus_tag="PKL60_07065"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNW95929.1"
                     /translation="MQKENTIAEVIQALLDTEQAFPTKLLYAFSDLEPRECTQLVEVW
                     EQIPIQRRRALLEDLSEMAEQDNLMLFEELGRIALEDDDPQVKISALDLLFQAEDTRL
                     LPRFLSLLKENESADVRAAAANVLGPYVCMGEFERLQAEQLKRIENALLQAYREDASE
                     LVRRRALESLGYSSRDEVPELLRSACTRAETAWLESALFAMGKSADEQWKDIVLAHLM
                     DENLPVRLQAVHAAGELALTEARASLLRVLEREFEVADLRREAVWALSQIGGEGVENA
                     LERLLERLEDDEDVELVEEALDQLNFTEDKELFSLLDMSLDEASGGEGILGLAEDEEE
                     TWEGHGYNPEEWNAYTSEEGFGLDLDEDEEDLDDDEFDDDDLL"
     gene            complement(3232..4308)
                     /locus_tag="PKL60_07070"
     CDS             complement(3232..4308)
                     /locus_tag="PKL60_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNW95930.1"
                     /translation="MTTFLTILQFVFVFGLLVFFHELGHFLMSKSLGIQIEEFGFGYP
                     PRLVKLFTWKGTDVTLNWIPFGGFVRPKGEGDETVEGGMAAAPAWKRFLVLVSGAGMN
                     FLIGILVLVLMFAAIGSPVQNQALITDIAPNSPAANAALQVGDVITAVNGTQLTNLEE
                     MSKLIQENLGKPLSFTLLREGESLSVELTPRVNPPEGEGSVGIWYTNAYAPLPFHKAL
                     GSAFQTFGYQTRQTLLLPYNLITGKVSGEDARLVGIKGIFDIFSNASELDQTSAVAAA
                     TPLPVFRLSVISTVSIALGLTNLLPIPALDGGQILFLLPEFLFKKRIPQQVANTINSI
                     FFLLLVGLMIFITVQDFLNPIIQP"
     gene            4443..7238
                     /locus_tag="PKL60_07075"
     CDS             4443..7238
                     /locus_tag="PKL60_07075"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNW95931.1"
                     /translation="MALIVALDLETTGLDPAQDAVIEIGAVKFSEKRVEAEYSTLINP
                     RKPISSFITNLTGITNSMVLNAPLLINTLPELVDFIGEAPILGQNIGFDMAFLQKAGV
                     LRANPTLDTYELASVLMPSAPRYNLGSLASQLGVLQPATHRALDDARATHAVFVELMA
                     QLERLPIDLVAEILRLSEGLNWKGELPFRWTLQKLTRQGIRPKKGSAESGALFASAKP
                     VQAKPLNPAEKPSPLDSETLAAALEPGGEFDRHFPEFEHRSQQVQMLRAVADAFSHQQ
                     HLLVEAGTGTGKSLAYLIPAAAWALQNGERVVISTNTIALQDQLVNKDIPDMIAALDL
                     NINVAVLKGRNNYLCPRRLNLLRKRQPENVDELRVLAKVLVWLQSSDSGDRNEINLNG
                     PVERTVWSRLSAEDEGCKLETCLKRTGGRCPYYRARTAADSAHILIVNHALLLADAAA
                     EGRVLPDYHYLIVDEAHHLESATTDAMSFRLRAPDVTRLVRELGSAEQGLFGRLLALG
                     QTQLKPSDLAAVTKAVEQATDQAFRLDNAINAYFRALESVMEELREGKPASQYAQQER
                     ITESKRKAPFWFEVEVRWEGAQVCLNDLLSTIKTIRESVRGLADSEDEEAEDLLGILG
                     TMTQNLLEIEAQVDGLTMKPDPNRIYWVEYDPLQRRLTLQAAPLEVGRLMEKTLWHTK
                     DSVILTSATLTTHGEFDYLRRRLNAEDANELTVGSPFDYENSALVYLARDVPEPKEVY
                     AFQKATEEAVVRLAKATGGRMLALFTSYSQLQKTSRAIEGPLAKADITIYEQGEGASA
                     SALLDVFKETPRAVLLGTRSFWEGVDVPGEALSVLVITKLPFDVPSDPIIEARSESFD
                     DPFNEYMLPEAILRFRQGFGRLIRTQTDRGVVAILDKRILSKQYGRLFMESLPGCTVM
                     EGFLADLPEKAARWLGA"
BASE COUNT         1549 a         2338 c         2192 g         1363 t
ORIGIN      
        1 cccgaagcct acatcccccg ccgcgacctc tacatcgaaa aagagaccga catcaacgag
       61 gatatcgagc gcctgcgcct ctccgccacc accagcctga tgtcccgcca ggacgtcatc
      121 atcgtggctt cggtctccgc catctacggc ctgggcgacc cggaagccta cggcaagagc
      181 gtgctcacca tccaaaaggg caaaatctac cgccgcaacg cgctcctcag gcagctggtg
      241 gatatccagt accagcgcaa cgactttgag ctcaagccgg gcgtgttccg cctgcgcggc
      301 gacacgctcg aaatcctgcc ggcctagcga gcgcgaagcg gtgcgcatca gctacttcgg
      361 cgacgaggtg gaagccatca cgcgcctgaa ccgcctgacc ggcgaggtga gcgccgaact
      421 ggaccaggtg gaaatctacc ccgccaaaca ctacatcacc gaagaacacc gcatggaaaa
      481 aaccctggcg gatatcgaag ccgagctgga agagcgcctg aaatacttcc gctccgaggg
      541 gcgcgtggtg gaagcccagc gcctggaaca gcgcacccgc tttgacctcg aaatgctgcg
      601 cgaggtcggg tcctgcgccg gcatcgaaaa ctactcccgc cacatcgacc agcgcgcccc
      661 gggtacccca ccctggaccc tgctggactt catgccctcg cgctatatgc tcgtgctgga
      721 cgaatcccac atgatggtgc cccaaatccg cggcatgtac aacggcgacc gctcccgcaa
      781 ggaaatcctg gtcgagtacg gcttccgcct gccttccgcg ttggataacc gcccgctaaa
      841 attcccggaa ttcgagagaa tgatgggcac cacactctat accagcgcca cccccggccc
      901 ctacgagatg ggcaaggccg ggcaggtggt cgagcaaatc atccgcccga ccggcctgct
      961 ggacccgcaa atcagcgtgc gccctagcca ggggcagatt gacgacctga ttggcgagct
     1021 gcgcaagcgc gtggagaaag gcgagcgcgc gctcgtcacc acgctcacca agcgtatggc
     1081 cgaagatatg gccaactacc tgatggaaat gggctttaag gtgcactacc tccactccga
     1141 ggtggagacg ctcgaccggg tcggcatcct gcgcgacctg cgcacgggcg tgttcgatgt
     1201 ggtcgtcggc atcaacctgc tgcgcgaggg gctggacctg cccgaggtct cgctggtggc
     1261 tatcctggac gcggataagg agggcttttt gcgttcctcc accgcgctta tccagaactt
     1321 cgggcgcgcg gcgcggcaca tcaacggcga ggtcatcctc tacgcggata agatgaccga
     1381 cgcgatgaaa agcgcgattg acgagaccaa ccggcggcgc gccaggcagg aagcctacaa
     1441 tatcgagcat cacatctccc cggtcggcat caccaaagcc gtgcgcgata tcaccgacca
     1501 gctggcaagc agcgcgggcg tagaacacac ggaaagccgc agcgcgcgcg agctggccgc
     1561 ccgcatggaa acccgggaaa tggaaaagat gatccgcgag atggaaaaac aaatgcgcgc
     1621 tgctgctgcc gagctgcagt tcgagcgcgc cgccatgctg cgcgaccagg tttacgagct
     1681 gcgcgcgctg ctggcggaga attccaagct gcccccctgg aagcagcaga acctgctggc
     1741 aggccgggaa gactgagctc tactcgtaaa tccgcacacg aaaacttgaa tccccaccgt
     1801 tggctggcag ccactccggc cggattcgcc gcacgaccga gccggaaggt ccttccgcca
     1861 gggcttccag gaacgcaagc aaatcctcgc gcgggccttc ggcttcggct tccaccgcgc
     1921 cgccgggcag gttgcgcacc cagccggaaa gtcccagccg gcgggcttgc tccaatgtga
     1981 agtagcggaa accgaccccc tgcacgtgcc cttccacgag cacatgcaag cgttggtttg
     2041 cttcagggtt tgcgtccacg gcgcggcctt tgctaatcct gccgtttaga gcaggtcgtc
     2101 atcgtcaaat tcgtcatcgt ccaggtcttc ctcgtcttca tcgaggtcca acccgaagcc
     2161 ttcttcgctc gtgtaggcat tccattcctc ggggttgtag ccgtgacctt cccaggtttc
     2221 ctcttcatcc tctgccagac ccaaaatgcc ttccccgcct gaagcctcgt ccaggctcat
     2281 atcgagcaga ctgaaaagtt ctttgtcctc ggtgaagtta agctggtcca gggcttcttc
     2341 caccaactcg acatcctcat catcttccag gcgttccagc aggcgctcaa gcgcgttttc
     2401 cacgccctcg ccgccaattt gggagagcgc ccagacggcc tcgcggcgca ggtcggcaac
     2461 ctcgaactcg cgttctaata cacgcaggag ggaagcgcgc gcttctgtga gcgccagctc
     2521 gccggcggcg tgcacagctt gcagccgcac cggcaggttt tcatccatca gatgtgctaa
     2581 tacgatatct ttccactgtt catccgcgga tttgcccatg gcaaagagcg cgctttccag
     2641 ccaggccgtt tccgcgcggg tacaagccga acgcagcagc tcgggcactt catcgcggct
     2701 ggagtagcca agggattcga gcgcccgccg gcggacaagt tcggaggcgt cttcgcggta
     2761 agcctgcagc agcgcgtttt caatgcgctt gagctgttcg gcctggaggc gctcaaactc
     2821 gcccatgcac acgtatgggc ccaacacgtt ggcggcagcc gcgcgcacgt ccgcgctctc
     2881 gttttctttc agcaggctca ggaacctggg cagcagccgg gtatcttccg cctgaaagag
     2941 caggtcgagt gcggaaatct tcacctgggg gtcgtcatct tccagcgcga tgcgcccaag
     3001 ctcttcaaag agcatcaggt tgtcttgctc ggccatttcg ctcaggtcct ccagcagcgc
     3061 gcgccggcgt tgaattggga tttgctccca aacttcaaca agctgcgtgc attcgcgcgg
     3121 ctccaggtcc gagaaggcgt acagcagttt ggtcggaaaa gcctgttcgg tatccagcag
     3181 ggcctggatg acttcggcga tggtgttttc tttctgcata tgggctccag tttaaggttg
     3241 gatgattgga ttaaggaagt cctgcaccgt gatgaaaatc atcagaccaa ccagaagcag
     3301 gaagaatatc gagttaatcg tgttggcaac ttgctgcggg atgcgcttct tgaacagaaa
     3361 ctccggaagc aggaacagga tttgcccgcc gtccaacgcg gggatgggca gcagattggt
     3421 gagcccgagc gcgatggaca cggtgctgat gaccgagagg cggaagaccg gcagcggcgt
     3481 ggcagccgct acggcgctgg tctggtccag ttcgctggcg ttgctgaaga tgtcgaagat
     3541 gcccttaatc ccgaccagac gcgcgtcctc cccggagacc ttgccggtaa tcaggttgta
     3601 gggcagcagc agggtttgcc tggtctggta cccgaaggtt tggaaagcgc ttcctaaagc
     3661 tttatggaag ggcagcgggg cgtaggcgtt ggtgtaccag atgccgaccg agccctcgcc
     3721 ttccggcggg ttcacgcgcg gggtcagttc cacgctcagg ctctcgcctt cgcgcagcaa
     3781 ggtaaagctg agcggcttgc caaggttctc ctggatgagt ttgctcatct cctccaggtt
     3841 cgtaagctgg gtgccgttga ccgcggtaat cacgtcgccg acttgcagag cggcgttcgc
     3901 ggcgggggag tttggggcaa tatcagtgat gagcgcctgg ttctgtaccg ggctgccaat
     3961 ggcggcaaac atcagcacca agaccaggat gccaatcaaa aagttcattc cggcgccgga
     4021 aaccagcacg aggaagcgct tccaggccgg cgcggcggcc atgccgcctt ccacggtctc
     4081 atcgccttcg cctttgggac gcacaaaacc gccaaacgga atccaattca gcgtcacgtc
     4141 ggtacccttc caggtgaaaa gtttcaccag gcgcggcggg taaccgaagc caaattcttc
     4201 aatctggatg cccagcgact tggacatcag gaaatgccca agctcatgaa aaaacactag
     4261 cagcccaaac acaaacacga actgcagaat ggtcaaaaaa gttgtcatca atgtaatgct
     4321 ccttcttact cggtattata cccagattag tgctccggct ctccgccgca aagcctggtg
     4381 tacaataccg cgcgggccgc gcgcaaatcc agcgggcacc cttatgaagt ataattttgc
     4441 gcatggctct catcgtcgcc ctcgatttgg aaaccaccgg cctggacccg gcgcaggacg
     4501 ctgtgataga gattggggca gttaagttca gcgaaaaacg cgtggaagct gagtactcca
     4561 cgcttatcaa cccccgcaag cccatttcca gcttcattac caacctgacc ggcatcacca
     4621 acagcatggt gctgaacgcg ccgcttttga ttaacacgct gcccgaactg gtggatttca
     4681 tcggggaagc cccgattctc ggtcagaata tcggcttcga catggccttt ctgcaaaaag
     4741 caggcgtgct gcgcgccaac cccaccctgg atacctatga gctggcatcc gtcctgatgc
     4801 ccagcgcacc acgttataac ctcggttcgc tggccagcca gctgggcgtg ctgcagcccg
     4861 ccacccaccg cgccctggat gacgcgcgcg ccacgcacgc agtcttcgtg gagctgatgg
     4921 cgcagttgga acgcctgccg attgacctcg tcgcggaaat tctgcgcctg agcgaaggtt
     4981 tgaattggaa aggcgagctc cccttccgtt ggacgctgca gaaactcacc cgccagggca
     5041 tccgtccgaa aaaaggcagc gcggaatcag gcgctttgtt tgcctccgcc aagcccgtcc
     5101 aggctaaacc gctcaacccg gcggaaaagc caagtccgct cgattcggaa acgctggcag
     5161 ctgccctgga acccggcgga gaatttgacc gccacttccc ggaatttgag caccgctcgc
     5221 agcaggttca aatgctgcgc gccgtcgcgg atgccttttc ccaccagcag cacctgctgg
     5281 tggaagccgg aacgggcaca ggcaagtccc tggcgtacct catcccggca gctgcctggg
     5341 cgctgcagaa cggagagcgc gtggtcatct ccaccaacac catcgccctg caggaccaac
     5401 tggtgaataa agatattccg gacatgattg ccgcgttgga cctgaacatc aacgtggccg
     5461 tgctgaaagg acgcaacaat tacctctgcc cgcgccgcct gaacctgctg cgcaaacgtc
     5521 agcccgaaaa cgtggacgag ctgcgcgtgc ttgcgaaggt gctggtatgg ctgcagtcca
     5581 gcgacagcgg cgaccgcaat gagattaatc tgaacggacc cgtagaacgc acggtttggt
     5641 cgcgcctgtc agccgaggat gaaggttgca agctggaaac ctgcctgaag cgcacgggtg
     5701 gacgctgccc ctactaccgc gcgcgcaccg ccgcggattc cgcgcacatc ctgattgtca
     5761 accacgccct gctgctggcg gatgccgcgg cggaaggacg ggtattgccg gattaccatt
     5821 acctcatcgt ggatgaagcc caccacctgg aatccgcgac gacggacgcg atgtctttcc
     5881 gcttacgggc gccagacgtg acccgcctgg tgcgggagct tggcagcgct gaacaaggcc
     5941 tgttcgggcg cttgttagcg ctgggccaaa cgcagctgaa gccttccgac ctggcagccg
     6001 taacgaaagc ggtggagcaa gccaccgacc aggcgttccg gctggataac gccatcaacg
     6061 cttacttccg cgcgcttgaa agtgtgatgg aagagctgcg cgagggcaag ccagccagcc
     6121 agtacgccca gcaggaacgc atcacggaaa gcaagcgcaa ggcgcccttc tggttcgagg
     6181 tggaagtgcg ctgggagggc gcgcaggtct gcctaaacga cctcttgagc accatcaaga
     6241 ccatccgcga gagcgtgcgc gggctggcgg actcggaaga tgaagaagcc gaagacctgc
     6301 tcgggattct tggcacaatg acccaaaact tgctcgagat cgaggcccag gtggacggcc
     6361 tgaccatgaa gcccgacccc aataggattt attgggtgga gtacgaccca ctccagcgcc
     6421 gtctgaccct gcaggcagcc ccgcttgaag ttggcaggct gatggaaaaa acactctggc
     6481 acaccaaaga cagcgtaatt ctgacttcgg cgacgctgac cacgcatggg gaatttgatt
     6541 acctgcggcg ccggctgaac gcggaggacg ccaacgaact cacggtcggt tcgccttttg
     6601 attacgaaaa ctcggcgctg gtttacctgg cgcgcgatgt gccggagccc aaggaagtct
     6661 acgctttcca gaaagcgacc gaagaagccg ttgtccgcct ggccaaagcc accggcgggc
     6721 gaatgctggc gctctttacg tcctactccc aactgcagaa gacctcccgc gcgattgagg
     6781 gaccgctggc gaaagcggat atcaccatct atgagcaggg agagggcgct tctgcttcag
     6841 ccctgctgga cgtgttcaag gagaccccgc gcgcggtgct gctgggtacg cgctcttttt
     6901 gggagggtgt agacgtgccc ggcgaagcgc tttcggtgtt ggtgattacc aaactgcctt
     6961 tcgacgtccc ctccgacccg attatcgagg cgcgctccga gagttttgac gacccgttca
     7021 acgagtacat gctgccggaa gcgattttgc gcttccgcca gggctttggg cgcctgattc
     7081 gcacccaaac cgaccgcggt gtggtcgcga tactggacaa acgcatcctg agcaagcagt
     7141 acgggcgcct gtttatggag tccctgcccg gctgcacggt gatggaaggt tttttggccg
     7201 acctgccgga gaaagctgcc cgctggctgg gagcgtgagc tgtcgtcccg ccccaattcg
     7261 tcattgcacc ctaattcgtc attagcccgc ggtactgaaa ttatccaggc accaatcaat
     7321 ccagaagggg gcgcggcaat ctgcctgtat gtttgttcac aggcagaccg aggtgcccgt
     7381 ccatttcatg gcgggcacag gcgccgccgc gctccgctcg cgatgacgaa cgctgagccg
     7441 ca
//
LOCUS       DAOQAD010000089         7437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_12043_length_7437_cov_6.645895, whole genome shotgun sequence.
ACCESSION   DAOQAD010000089 DAOQAD010000000
VERSION     DAOQAD010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12043_length_7437_cov_6.645895"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1507
                     /locus_tag="PKL60_07080"
     CDS             188..1507
                     /locus_tag="PKL60_07080"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNW95932.1"
                     /translation="MDFDHFAFHPQIKAGISAADYADPTPIQEKTIPVILAGKDVLGL
                     ARTGTGKTAAFVLPILQQLMRGPRGKLRALILAPTRELAEQTHTFIKLLGRQTGLRSI
                     TIYGGVGQKPQKDALKEGVDIVVACPGRLLDLKNQGALDLSRIQVLVLDEADQMFDMG
                     FLPSIRRIIEALPQKRQTLLFSATMPEEIRKLANQILHNPVTIEISITEPLHSITHAV
                     YLAEPARKEEMLLHLLKEAGEGSVLVFIRTKHRAKKLAFQLQQAGISATSLQGNLSQS
                     QRDSAMRAFRDGKVQVLVATDIAARGIDVSRVSHVINYDLPDTPEAYTHRTGRTGRME
                     RLGTALSLVSQEDLDMLRTIERLMGKKLELRTMDGFETSFNINSPALKVVRQTNRKPE
                     GVKASRLTDANLNARRKAGRESGISAERSQKSQRRVYNKNRYSSYET"
     gene            1645..3099
                     /locus_tag="PKL60_07085"
     CDS             1645..3099
                     /locus_tag="PKL60_07085"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNW95933.1"
                     /translation="MKQELKKNIDSLMAQGATYVDARWYPVEESNSLLMWNGNLKDLN
                     ASSQSGLGVRVLYRGAWGFSAASSLEDTAAIFSKALDNARTAAERVDFPVRLAEKDAV
                     RASFVSPNRIDPFTVPLTDKLEFLRQMDARLNQPGVAQRVAAVDFVRRQIVFLDSEGS
                     EIEKTIIDVYPFFQVMGVDKDGEVQTRKYTSTRLGNTRGWESLDPEYFSGTAERLVRE
                     LNQLLVAENCPKDTRSVILLPGIMFLQTHETIGHPLELDRILGYELAFAGGSFVRLED
                     FGKLRYGSEKLTVRADATLENSPGSFGFDDDGVPAQNNLLIDKGILVGAISGRQMVEE
                     ANARARRQIFSGSSGANRATAFYRVPIERMTNINVDPGTDGDLNDIVRGTERGVILDG
                     DKSWSIGSNREQFHFATDIGWLVEDGEVKGVLKNTTYKGDTLKFYNSLSAVGDERTWQ
                     VMYVDNCGKGQPNQIMQLGHGVPVCRFDHVTVGE"
     gene            3102..4487
                     /locus_tag="PKL60_07090"
     CDS             3102..4487
                     /locus_tag="PKL60_07090"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNW95934.1"
                     /translation="MQHNVIALLQELRAYALEKGIRAAIFYHEEESYLMRFANSAISL
                     NTNEHLVRLQIDVYDGNKRAEIMLLADPGQIEPLKRGIDKAAEMAALAEPLSYTPSLP
                     VYTETVVDMQAYDPFLAVTTNAERLEFFNTLARGLETEELKLSGVFSNGITTTAQIST
                     ESEPSQYFACTDAAITAVLSHQRLKWEVNALQSAQAKTDLDAERLHAELAYLVRQYSE
                     QPAVQLPLGKYTVVFGAAAVGDILEMLNWFGPNGGMMKRGYSFLSEAQLGQPVFSPLF
                     TLTDDPLCLPTYPVSRDLMGKRRERFPLFEEGVLKAFFWNQDDADEFGAQATGHTLYH
                     NNLVLSGGEKSVGSLEALAALPREEDILYIPYLHYMNIVNPTAGILTGSSRFGALLLR
                     KDGSVAVPFNVRITQSFADLFGERLAWLSREQIAHNVSNSYGMRNPSALLVPRFLCVR
                     GIEISHSNSSY"
     gene            5162..>7437
                     /locus_tag="PKL60_07095"
     CDS             5162..>7437
                     /locus_tag="PKL60_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNW95935.1"
                     /translation="MRSKTIRILALIIVLAMVASPVSGLKLNTVAAQGGLPGRSDLVP
                     TGNTPVEKYSAVDAADVVDKTEVSRYIVLFEGASLSRAKGSAGINSIEGRNYLDSLAV
                     NRQTMLAKAESLLGRSIQVRYIYDVVLNGVSVELSAQEAEDLQSLPGIRKVLKDQTYT
                     IDTEVGPEWIGAGDIWDGAAVPDGIGSEGEGVLVGIIDTGINFDHPSFSDTPADGFEY
                     EWTGDYLGVCAPDGDPEYADACNDKIVGAWSYTTDAPTDFVTPEDAEGHGSHTASTVA
                     GNFVDVNFYGAEVTISGVAPHAQIISYDVCYPNEANGACEGEDAIAATQQAILDGVDV
                     INYSISGGTYPYSDGVELAFLDATAAGITVSTSAGNSGPTAATVAHRSPWLLSTAATT
                     HNRKFSSEVNFSNPLYQGITTLAGEIPFTTDVVDSEVKFAGEDGNPLGCLDPGYTTPN
                     FYDGAIALIKRGTCNFSEKIIAAEAAGASGVLIFTNANPPGAMSVSGTSIPGVMLNIP
                     GTVGDALAAWVASETDETVSIAGYALDDAFADIMADFSSRGPNTTFDVLKPDIAAPGV
                     EILAAVADGVIEPDGETELALYGGTSMASPHDAGSAALLTALHPDWTPAEIKSALMLT
                     AYDGLVKEDKITAADPFDIGAGRIQLELAGLTGLVMDESIDNYEAADPDLGGDVRTLN
                     TASVYDSACVGECSWTRTFTSVADLPATYTVSAPDWITVAPASFTINPGATQEITITA
                     DVSSYAPNEWVFANIEFLT"
BASE COUNT         1648 a         2176 c         2119 g         1494 t
ORIGIN      
        1 cgtgtggggt tagttgaaca gaaaatagac tttcaacgga ccaggcaaag cctggttttc
       61 tattgtacat ggaccgctcc actgattatg ggacaattgc gcagcagggc gagggagcgg
      121 gcgcgttgag tattgacgcg tacagagcct gatgctgtca agcatgctgc gcgtaaagga
      181 atatttcgtg gattttgatc attttgcttt tcacccgcaa atcaaagcgg gcatttctgc
      241 ggcagattat gccgacccaa cccccattca ggaaaaaacc atcccggtca ttctggcggg
      301 caaggatgtt ttaggcctcg cccggactgg aaccggaaag acggccgcct ttgtgctgcc
      361 catcctgcag cagctcatgc gcggtccgcg cgggaaactg cgggctctta ttctcgcgcc
      421 aacgcgtgaa ctggcggaac agacacatac cttcataaaa ttgcttggcc gccaaaccgg
      481 cctgcgcagc atcaccatct acggcggggt ggggcaaaag ccgcagaaag acgcccttaa
      541 agagggtgtg gacattgtgg ttgcttgccc cggccgcttg ctggacctga aaaaccaggg
      601 cgcgctggac ctgagccgca tccaggttct cgttctggat gaggccgacc agatgtttga
      661 catgggcttt ttgccttcca tccgccgcat catcgaagcg ctgccccaaa aacgccagac
      721 cctgctgttc tctgccacca tgccggaaga aatccgcaag cttgccaacc aaatcctcca
      781 taacccggtg accatcgaaa tcagtatcac tgagccgttg cattccatca cccatgcggt
      841 ttatctggcc gaacccgccc gcaaggagga gatgctcctg cacctgctca aagaggcggg
      901 cgagggcagc gtgctggtct tcatccgcac caaacaccgc gcgaaaaagc tggctttcca
      961 gctgcagcag gccggcatct ccgccacctc gctgcagggc aacctttcgc aatcccaacg
     1021 cgacagcgcc atgcgcgcct tccgggacgg aaaagtgcag gttctggtcg ccaccgatat
     1081 tgccgcacgc gggattgacg tttcgcgcgt ctcgcatgtg attaactacg acctgccgga
     1141 taccccggaa gcctataccc accgcaccgg ccggaccgga cgcatggagc gcctgggaac
     1201 cgcgctttct ctcgtctccc aggaagacct ggacatgctg cgcacgatag aacgcctgat
     1261 gggaaaaaag ctggaactgc gcacaatgga cgggtttgag acaagcttca atatcaacag
     1321 cccggcgctg aaggttgtca ggcagacgaa cagaaagccg gagggcgtga aggcctcgcg
     1381 cctgacggac gcgaacctca acgcgcgccg aaaagccggc cgagaaagcg ggatatccgc
     1441 ggaacgttcg cagaaaagcc agcgcagggt ctacaataaa aaccgctatt catcgtatga
     1501 gacatgagcc gtccccctcg gggcctggaa gcaagtttgc catctgcgcg cgaatttgag
     1561 ggcattcagg acttcctccg gtataatcag cctatgtttc gccagggcga tggaaacctg
     1621 gtgaaccgat aaaggagaag ggctatgaag caggaactta aaaagaacat cgacagtttg
     1681 atggcgcagg gagctaccta cgtggatgcg cgctggtacc ccgttgaaga gagcaacagc
     1741 ctgctgatgt ggaacggcaa cctcaaggac cttaacgcgt cttcccagag cgggctgggg
     1801 gtgcgggtgc tgtatcgcgg cgcctgggga ttttccgccg cgtccagcct ggaagacacc
     1861 gccgcgattt tctcgaaagc gctggataac gcccgcacgg cagccgaaag ggtggacttc
     1921 ccggtgcgcc tggcggagaa ggacgctgtg cgggcttcgt ttgtcagccc gaaccggatc
     1981 gaccctttca cagtcccgct gacggacaag ctggaattcc tgcggcagat ggatgccagg
     2041 ctgaaccagc cgggagtggc tcagcgcgtg gcggcagtgg attttgtcag gcgccagatt
     2101 gtattcctgg actcggaagg cagtgaaatc gaaaaaacga ttatcgatgt atacccattc
     2161 tttcaggtga tgggcgttga taaagacggg gaagtgcaga cccgcaagta cacatccacc
     2221 cgcctgggga atacccgcgg ttgggaatcg ctggacccgg agtatttcag cggcacggcc
     2281 gaacgcctgg tgcgcgagct taaccaattg ctcgtggcgg aaaactgccc caaagacacc
     2341 cgctcagtaa tcctgctgcc gggcatcatg ttcttgcaga ctcacgagac catcggccac
     2401 cccctggagc tcgaccgcat cctcgggtat gagctggctt tcgccggcgg ttctttcgtg
     2461 cgcctggaag actttggcaa gctgcgttac ggctccgaga agttgactgt gcgcgcggac
     2521 gctaccctgg aaaattcccc cggcagtttc ggctttgatg acgacggcgt cccggcacaa
     2581 aacaacctgc tgattgacaa aggcatcctg gtgggcgcga ttagcggcag gcagatggtg
     2641 gaagaagcca atgccagagc ccgccgacag attttcagcg gcagcagcgg cgccaaccgc
     2701 gccacggctt tctaccgcgt cccgattgag cgcatgacca acattaacgt ggaccccggc
     2761 acggacggcg acctcaacga tattgtgcgc ggcaccgaac gcggcgttat cctagacggc
     2821 gataagagct ggtccattgg ctccaaccgc gaacagttcc actttgccac cgatatcggc
     2881 tggctggtgg aagatgggga agtgaagggc gtgctgaaga acaccaccta caaaggtgac
     2941 accctcaagt tctacaattc actttccgcg gtcggcgacg agcgcacctg gcaggttatg
     3001 tacgtggata attgcggcaa ggggcagccc aaccagatta tgcagctggg gcacggcgtg
     3061 ccggtctgcc gttttgatca tgtaacggta ggagaataac catgcagcac aatgtcatcg
     3121 ctttactgca ggaactgcgc gcgtacgctc tggagaaagg gatccgcgcc gccatttttt
     3181 accatgaaga agaaagctac ctgatgcgct tcgccaattc agcaatttct ctgaacacaa
     3241 acgagcatct ggtccggctg caaatcgatg tatatgacgg aaacaaacgc gccgaaatca
     3301 tgctgctggc cgaccccggc caaattgagc cgctgaagcg cggaattgac aaagccgccg
     3361 agatggcagc cctggccgaa ccgctgtcct acacgcccag cctccccgtc tacacggaga
     3421 ccgtggtcga catgcaggct tacgacccgt tcctggcagt caccaccaac gctgagcgcc
     3481 tggagttttt caacacactt gcccgcggac tggaaactga agagctcaag ctttccggag
     3541 tcttttcgaa cggcattacc accacagccc aaatcagcac agaaagcgaa cccagccagt
     3601 actttgcctg caccgacgct gccatcaccg cggtgctctc gcaccagagg ctgaaatggg
     3661 aagtaaacgc gctgcaatcc gcccaagcca agactgacct ggacgcggaa cgtctgcacg
     3721 cggaactggc ctacctggtg cggcagtaca gcgagcagcc ggccgtccaa ctgccgctgg
     3781 gcaaatacac cgtcgtgttc ggcgcggcag ctgtggggga tatcctggaa atgctgaact
     3841 ggttcggacc caacggcggg atgatgaagc gcggctattc tttcctgagt gaagctcagc
     3901 tgggacagcc agttttctcc ccgctcttta cgctgacgga cgacccgctc tgtctgccga
     3961 cctatccggt ctcgcgcgac ctgatgggca aacgccgtga acgcttcccg ctctttgaag
     4021 aaggggtctt gaaggccttc ttctggaacc aggacgacgc ggacgagttc ggcgcccagg
     4081 cgaccggcca cacgctctac cacaacaacc tggtgctctc gggcggcgaa aaatcggtcg
     4141 ggagcctgga agcgctggca gccctgccgc gtgaggaaga tatcctctat atcccttatc
     4201 ttcattatat gaatattgtc aacccgacag ccggcatcct tacgggcagt tcgcgctttg
     4261 gggcgctgct gctgcgcaag gacggcagcg tggcggtgcc tttcaacgtg cgcatcacgc
     4321 agagcttcgc agacctgttc ggggagcgcc tcgcgtggct ctcgcgcgag cagattgcgc
     4381 ataacgtctc gaacagttac gggatgcgca acccatccgc gctgctcgtt ccgcgctttt
     4441 tgtgcgtccg gggaatcgaa atatcgcatt ccaacagctc atactaaggt tgtcgggtcc
     4501 gaacagcgcc agaaagccct ggaaatgctt ttattgcagg gaaagtgtcc ggggaaagtg
     4561 tcaaggctaa atagaaatgt cctatagctg gcaagttaga aatgtcctga ttcgtggagg
     4621 tagccgtcag gaagggtttt tcctcgaaac gaagccccgt atttcttctt tgcgatcatg
     4681 tttcacggtt gcccccgaac cccgagcctt ccccagcggc aagcggctct gttttgcctc
     4741 agccaagctg ctgggaaggc gaggaagagg ggaaaagcgg ctcccttgtg atcattcctt
     4801 cggaagaaaa cttggaggta acgaatgagg ttttcatagc tcctaatttt aatcaggaca
     4861 tttctatttt gctactttag gacattatca tgttgctctt acacattttt gcggggaaag
     4921 gcttgttttt tctttcaggt ggtatactat tcgcatacac aatacgcaat tgtgttttct
     4981 gtcgcaggta ctgacactat tcgcaaaagt aagttacata ttacggaagg aggagcaata
     5041 tttgccgtaa atcgaatcgc ttccaagctc tcagcccaca ggttagtgcc tttttacttg
     5101 gctggtctga gaaattcctg tctctgtcac aaatccaaac taaacccaat ggagtgaact
     5161 gatgagatca aaaactatcc gaatcctcgc attaatcatt gttctggcga tggtcgcctc
     5221 tcccgtcagc ggcctgaagc taaataccgt cgcagcccag ggtggcctgc ccgggcgcag
     5281 cgatctggtc cccaccggca atacgccggt tgagaaatat tcagcggtcg acgcggctga
     5341 tgtggtcgac aaaaccgaag tctcgcgcta cattgtgctt ttcgaaggcg cctcgctcag
     5401 cagagccaag ggcagcgcgg gcatcaacag cattgaaggc cggaactacc tggacagcct
     5461 ggcagtcaat cgccagacga tgctggcgaa ggcggaaagc ctgctggggc gttccatcca
     5521 ggtccgttat atctacgacg tggtcttgaa cggcgtctcc gttgagctga gcgcgcagga
     5581 agccgaagac ctgcaaagcc tgcccggcat ccgcaaagtc ctgaaagacc agacctacac
     5641 catcgacacc gaagtgggtc cggaatggat tggcgcgggc gacatttggg acggcgcggc
     5701 tgttcctgac ggtattggca gcgaaggcga aggcgtactg gtcggcatca tcgataccgg
     5761 catcaacttt gaccatcctt ccttcagcga tacgcctgcc gacggctttg aatatgaatg
     5821 gaccggcgat tacctgggcg tctgcgcccc cgacggcgat ccagagtatg ccgatgcctg
     5881 taacgacaaa attgtcggcg cctggagcta tactaccgac gctcccaccg acttcgtgac
     5941 cccggaagac gcggaaggcc acggctccca caccgccagc accgttgccg gcaattttgt
     6001 ggatgtgaat ttctacggtg ctgaagtcac catttccggc gtcgcgccgc acgcgcagat
     6061 tatttcctat gatgtctgct acccaaatga ggcgaatggc gcatgcgaag gtgaagacgc
     6121 cattgcagct acccagcagg caattttgga cggcgtggat gtgatcaact actctatctc
     6181 tggcggcaca tatccgtaca gtgatggcgt ggaactggca tttctggatg ccactgccgc
     6241 aggcattacc gtttccacgt ccgctggcaa cagcggcccg accgccgcaa ccgtggcgca
     6301 ccgctccccc tggctgctca gcactgctgc tacgacccac aaccgcaagt tcagcagcga
     6361 ggtaaatttc tccaatccgc tgtatcaggg tattaccacg cttgccggtg aaatcccctt
     6421 tacgaccgat gttgtggaca gcgaggtaaa atttgccggt gaagacggca acccgttggg
     6481 ttgtttggac ccgggttaca ccaccccaaa tttctacgat ggagcgatcg ctctcatcaa
     6541 gcgcggaacc tgtaacttct cagaaaagat tatcgcggct gaagccgccg gcgcctcagg
     6601 cgtgttgatt ttcacgaacg ctaacccacc cggcgcgatg agcgtctccg gtacaagtat
     6661 ccccggcgtg atgctgaaca tccccggcac cgttggcgac gcgcttgccg cctgggttgc
     6721 cagcgagacg gatgaaaccg tcagcatcgc ggggtatgcg ctggatgatg cttttgctga
     6781 tattatggcg gacttcagct cccgcggtcc caatacgacc tttgacgtgc tgaaaccgga
     6841 tatcgccgcg ccaggcgttg aaattctggc cgcggtcgcg gacggggtta ttgaacccga
     6901 tggcgaaact gagctcgccc tgtatggggg cacctcgatg gccagcccgc acgatgccgg
     6961 ctctgccgcg ctcctgaccg cgctgcaccc cgattggacc cccgcggaaa tcaagtccgc
     7021 gctgatgctc acggcatatg acggtttggt gaaagaagac aaaatcaccg ccgccgaccc
     7081 gtttgatatt ggcgccggcc gcatccagct ggaactggct ggcctgaccg gtctggtgat
     7141 ggatgaatcc atcgataact acgaagctgc cgacccggac ctgggcggcg atgtgcgcac
     7201 ccttaacacc gccagcgtgt acgacagcgc ttgcgtgggc gaatgcagct ggacgcgcac
     7261 cttcaccagc gtggcggacc tgcccgcgac ctatacggtc agcgctccgg actggatcac
     7321 tgttgctcct gccagcttta cgatcaaccc cggcgcgacg caggaaatta ccatcaccgc
     7381 ggatgtttcc tcgtacgcgc ccaatgagtg ggtgtttgcg aacattgaat tcctgac
//
LOCUS       DAOQAD010000090         7225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_12557_length_7225_cov_6.172245, whole genome shotgun sequence.
ACCESSION   DAOQAD010000090 DAOQAD010000000
VERSION     DAOQAD010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12557_length_7225_cov_6.172245"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..563
                     /locus_tag="PKL60_07100"
     CDS             195..563
                     /locus_tag="PKL60_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNW95936.1"
                     /translation="MPIRIKRIYEGYNPQDGWRVLVDRIWPRGVSKAAARLDDWRKDL
                     APSTELRTWFCHKVELFDEFSKRYRAELDENASAQTGIQQILEKSRSGQVTLLYSAKD
                     TEHNQAVVLSAYLNEKASRE"
     gene            738..1010
                     /gene="rpsO"
                     /locus_tag="PKL60_07105"
     CDS             738..1010
                     /gene="rpsO"
                     /locus_tag="PKL60_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNW95937.1"
                     /translation="MSLNQTEKTEIIKKYERQPGDTGSPEVQIALLTTRITQLTNHLR
                     ANRHDESSRRGLLKLVGQRRRLLAYLRQKDYDRFLAITESLNIRKK"
     gene            1204..3450
                     /locus_tag="PKL60_07110"
     CDS             1204..3450
                     /locus_tag="PKL60_07110"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNW95938.1"
                     /translation="MKPQAIYFKTLLGGKELTFETGRLARQAGGAVTLRQGDSIILAA
                     ATMGGVREGTDFFPLTVDYEERMYAGGRIPGSFFRREGKPSTDATLIARLTDRPLRPL
                     FPEGMRNEVQVVLMSLSADTENQLDMLAINAASAALMISDIPFGGPVGAVRIGCVDGK
                     LVVNPSISEMEISTLDLRVAGTREAILMVECGAQEVSEDLMVEALQLAHQEIQGVVEL
                     QERMAREVGKQKKEVELKVLDPELISQARASVETRLKAILDQPYSKENMYGGTAELRK
                     TYLEEAAAGAELTPEQSAAFGAAFDAAEKAIVRDRILYEGKRPDNRSINEIREITCEV
                     DISPRAHGSGLFTRGETQVLTLATLGTPKEAQELDNLVETESKRYMHHYNFPPYSTGE
                     VKRLGGQSRREIGHGALAERALVPVIPDEKSFPYTLRLVSEVLASNGSSSMASVCGST
                     LALMDTGVPIKAPVSGVAMGLIKSDERYVILTDILGAEDHLGDMDFKVAGTREGITAL
                     QMDIKITGITPGIMAEALERARAARFSILDIMLETIAVPNPNLKPHAPRIETVKVPVD
                     KIGAIIGPGGKNIRALQEETHTKIDIDDDGTVYIASTDGVGFEQAKERILSMVEVPEI
                     GRIYTGKCVRVTDFGAFIEILPGIDGMVHISQLASERVNKVEDIVQLGDELSVMVTDI
                     SPEGKIRLSRQALLEGWSVEEAREHDNANRKKSSSGGSGSGGSRGGSRGSDRGDRGGD
                     RGSFNRRR"
     gene            complement(3554..4615)
                     /locus_tag="PKL60_07115"
     CDS             complement(3554..4615)
                     /locus_tag="PKL60_07115"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95939.1"
                     /translation="MRNFRVAVLANLKKNAPQLEGASFDKWDDLDSEFTVEAICEAIR
                     SKGNEAIFLEADESIIDTVREYKPDICFNISEGHYGDSREAQIPAILEKLQIPYTGSK
                     VLCLALTLDKSMTKRILAWHGLPTPEFQSFERLDEPLDEGMTFPLFVKPSREGTGMGI
                     SYDSIVHNEDELRKQLEFILRTYHQSALVEKFIEGREITVGMVGNLIGPVARRIPVNE
                     DAQRIQAGLHFMPPMEVDLKPYLLTDGVYANRLKTALAADLTYLCPAPLDEEMVDELN
                     WLAAAVFRVTGALDVARVDFRLDVNDNFKPYILEINPLPGLTPVISDLVIEAAAAGID
                     HTELVNMILYTALDRYKML"
     gene            complement(4734..6050)
                     /locus_tag="PKL60_07120"
     CDS             complement(4734..6050)
                     /locus_tag="PKL60_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW95940.1"
                     /translation="MDNERLHKLQVRAWTMYDWANSAFATSVMGAILPNFFGAYIATE
                     GSLALWGYTVAIGSLIAALLSPVLGAIADFRGSKKKFLALFAGLGILSTALLFFVDKP
                     EEWLLCCILYILGTIGFAGSLVFYDSLLPHVAHEDEIDQVSSRGYAMGYIGGGILLLV
                     NVVMIFLGPKLTPNLGERGATMLWMRLSLASVALWWAVFSIPLFRHVQEPARMAEVGE
                     LGKNVWKVSITRFTTAFKEIQKFKDLFWFLLTFLVYANGIGTIITMAAAFATDLGFST
                     ITILGTFLMVQFVAAPFAILFGKLAKKMGNKKAITISLLIYSVVAIAGYFMTKEWHFY
                     VLGFGVAMVQGGSQALSRSLVGKLMPKSKSAEFYGFFSISEKFNTVIGPATMAVISQL
                     TGSGRWGIVSLVVFFGAGILMLRGVNVERGIARAEEEDAKMIAVES"
     gene            complement(6095..7117)
                     /locus_tag="PKL60_07125"
     CDS             complement(6095..7117)
                     /locus_tag="PKL60_07125"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNW95941.1"
                     /translation="MSESSAPEPAFRIGSIPVYGRLILSPMDGISDPPFRWITRRLGS
                     ALSISEFINTLDYANQKHYQVSRLSFRAAERPFGVQLLDNDPFRMADCAAEIYSEYQP
                     DFFDVNLGCCTASVTSRGAGAGMLRTPAKVAQAFQELSKAVPVPLTAKLRLGWDEDSL
                     NYRQIAEIAVENGACVIALHGRTAKMAYTGKARWQPIAELKASLPVPVIGNGDVTSPT
                     RAHQIFAQTGCDAAMVGRAAKANPFIFSWRDRSEVSPEEIYGLIRYQLEAMQAFHPAG
                     ALLPFRKFLKAYLEPYALPKEQLRALLTSGEEAELLRLIDQVFPAQGVMDLAGAEQDF
                     RNRMHE"
BASE COUNT         1617 a         2104 c         2083 g         1421 t
ORIGIN      
        1 ttgattaaca tatcgggaga tcgcttggtg cctcgcgatg acgaatgaaa tgctcctgct
       61 gatctccgag accatgctaa aacgaaagcc ttcccatccc gtccaagctt attcacgctg
      121 tgtctcgcta ttacgcctcc gctgcgataa actcaaatca gcaggccttt ttcatcgcct
      181 taaggagcgc caaaatgccg atcaggatta agagaatcta tgagggctac aacccgcagg
      241 acggttggcg tgtcttggta gaccgcatct ggccgcgcgg cgtctccaaa gccgcggccc
      301 gcctggatga ctggcgtaag gacctggcgc ccagcacaga gctgcgcacc tggttctgtc
      361 ataaggtgga gcttttcgat gaattcagca aacgctaccg cgctgagctg gacgaaaacg
      421 cttccgcgca aaccggcatc cagcaaatcc tggaaaaaag ccgcagcggg caggtgactt
      481 tgctctactc ggccaaagac accgagcaca accaggccgt tgtgctgtcc gcctacctga
      541 atgaaaaagc atccagggag tgagcttgct gttcctccca atcagccgcc cggccgccca
      601 cccagtcctg cacgagccca gcccgccagg cttgcgtttt tcacttttct ggtataatct
      661 caaactgtga ctggtccggt gagcggttcg cgctgcttgc caggagactt gcataaattc
      721 tttcaggagc agacgtcatg tctttaaacc aaaccgaaaa aaccgaaatc atcaagaagt
      781 acgaacgcca acccggtgat accggctcgc ctgaggtgca aattgccctc ctgaccaccc
      841 gcatcaccca gcttaccaac catctgcgcg ccaaccgcca tgatgaaagc tcgcgccgcg
      901 ggctgctcaa actggtcggt cagcgccgcc gtttgttggc ctatcttcgc cagaaggatt
      961 acgatcgttt cctcgccatc accgaatcac tcaacattcg gaaaaagtag tcagctaccg
     1021 ttttcccacg gcgacccaac cgcgcagctt ggtccgcagg cagtacagcc tgcttgcgga
     1081 cctgaactga cgcggttatt attggattaa acaaactatg ccagtcgggt attgaaaggt
     1141 gatcatcaga tatgacaatc agctttcagt ccctgtactg gcgcaaccag tcgaaaggac
     1201 actatgaaac ctcaagctat ttattttaag accctcctgg gcggcaaaga actgaccttt
     1261 gaaaccggca ggcttgcccg ccaggccggc ggcgcggtca ctttgcgtca gggcgattcc
     1321 atcatcctgg ccgcggccac catgggcggc gtgcgtgaag gcacggattt cttcccgctg
     1381 accgtagact acgaagagcg tatgtacgcc ggcggacgca tccccggctc tttcttccgc
     1441 cgcgaaggca aacccagcac ggacgcgacc ttgatcgccc gcctgacgga ccgcccgctg
     1501 cgcccgctct tccccgaagg catgcgcaac gaagtgcagg tcgttttgat gtcgctctcc
     1561 gcggacaccg aaaaccagct ggatatgctg gccattaacg ccgcttcggc cgcgctgatg
     1621 atttccgaca tcccctttgg cggaccggtc ggcgcggtgc gcattggctg cgtggacggc
     1681 aaattggtcg tcaacccctc gatttccgag atggaaatct cgacgctcga tttgcgcgtt
     1741 gccggcaccc gcgaagccat cctgatggtg gaatgcggcg cgcaggaagt cagcgaagac
     1801 ctgatggtgg aagcgctgca gcttgcccac caggaaattc agggcgtcgt ggaactgcag
     1861 gaacgcatgg cgcgcgaagt tggcaagcag aaaaaggaag tggaactgaa agttctggac
     1921 cccgaactga ttagccaggc ccgcgcaagc gttgaaaccc gcctgaaagc catcctggac
     1981 cagccttaca gcaaagaaaa catgtacggc ggcaccgccg aactgcgcaa gacctacctg
     2041 gaagaagctg ccgctggcgc ggagctgacc cccgagcaga gcgccgcttt cggcgccgcc
     2101 tttgacgcag ccgaaaaagc catcgtgcgc gacagaatcc tgtacgaagg caaacgtccc
     2161 gataaccgca gcatcaacga gattcgcgaa atcacctgcg aagtggatat cagcccgcgc
     2221 gcgcacggct ccggcctgtt cacccgcggc gagacccagg tgctcaccct ggcgaccctg
     2281 ggcacgccca aggaagccca ggaactggac aacctggtgg aaaccgaatc caagcgctac
     2341 atgcaccact acaacttccc gccctacagc accggcgaag tgaaacgcct cggcgggcag
     2401 tcccgccgtg aaatcgggca cggagctctg gcggaacgcg ccctcgtgcc ggtcattccg
     2461 gatgagaaga gcttccccta cacgctgcgc ctggtctcgg aagtgctcgc ttccaatggc
     2521 tcgtcttcga tggcgtctgt atgcggttcc accctggcgc tgatggatac cggcgtgccg
     2581 attaaggcgc ctgtttccgg cgtcgccatg ggcttgatta aatccgacga gcgctatgtc
     2641 atcctgaccg acatcctcgg agcggaagac cacctgggcg acatggactt caaggttgct
     2701 ggcacgcgtg agggcatcac cgcgctgcaa atggacatca aaatcaccgg tatcaccccc
     2761 gggattatgg cagaagccct ggaacgcgcc cgcgcagccc gcttcagcat cctggatatt
     2821 atgctcgaga ccattgcggt cccgaatccg aacctgaaac cgcacgctcc gcgcatcgag
     2881 actgtcaagg tccccgtgga caaaatcggc gcgattatcg gccctggcgg caagaatatc
     2941 cgcgcgctgc aggaagaaac ccatactaag attgatatcg acgatgacgg cacagtgtac
     3001 attgcctcta cggacggcgt gggctttgaa caggccaagg aacgcatcct cagtatggtg
     3061 gaagtgccgg aaatcggcag gatttatacc ggcaagtgcg tacgcgtcac cgattttggc
     3121 gctttcattg aaattctgcc cggaatcgac ggcatggtgc atatctccca actcgccagc
     3181 gaacgcgtga acaaagttga agatatcgtc cagctgggcg atgaactttc tgtgatggtt
     3241 accgacatca gcccggaagg gaaaatccgc ctcagccgtc aggccctcct ggaaggctgg
     3301 agcgtggaag aagcccgcga acacgataac gccaaccgca agaagagctc ttccggcggg
     3361 tctggttccg gcggcagccg cggcggaagc cggggcagcg accggggaga ccggggcggc
     3421 gaccgcggaa gcttcaaccg cagacgctaa gctttacttg ctcaaaacga tactggctgc
     3481 catttggcag ccagtattta ttttgtccaa gcaagcccgt cagcgcgcca ggcggttgcg
     3541 ccgcggaaag aggctacagc attttgtagc ggtcgagcgc ggtatacaga atcatgttca
     3601 ccaattcggt atggtcaata ccggccgcgg cggcttctat caccaggtcg gaaatgaccg
     3661 gcgtgaggcc gggcagcgga ttgatttcga ggatgtaggg cttgaagttg tcgttgacgt
     3721 ccaggcggaa atccacgcgc gccacgtcca gcgcgccagt cacgcggaaa accgccgccg
     3781 ccagccagtt caactcatcc accatttctt cgtcaagggg cgcagggcaa aggtaggtca
     3841 ggtcggctgc cagggcggtc ttcaggcggt tcgcgtaaac cccatccgta agcaggtagg
     3901 gcttcaggtc cacctccata ggcggcataa agtgcaggcc ggcctggatg cgctgcgcgt
     3961 cttcgttgac cgggatgcgc cgggcaaccg ggccaatcag gttcccgacc atgccaactg
     4021 taatttcgcg cccctcgatg aacttctcga ccaacgcaga ctggtggtag gttcgaagga
     4081 tgaactccag ctgtttgcgc agttcatcct cgttatgcac gatggagtca tagctgatgc
     4141 ccatacccgt gccttcgcgg ctgggcttta cgaaaagtgg gaaagtcatg ccctcgtcca
     4201 acggctcgtc caggcgttca aaggattgaa actccggtgt gggcagcccg tgccaggcca
     4261 gaatgcgctt ggtcatcgat ttatccaggg tcagggccag gcagagcacc ttggaaccag
     4321 tataggggat ttgcagcttt tccaggatgg cggggatttg cgcttcgcgc gagtccccgt
     4381 agtgcccttc gctgatgttg aagcaaatat cgggtttata ctcccggaca gtatcaatga
     4441 tgctctcgtc cgcttccagg aatatggctt cgttgccttt actgcggatt gcttcgcaaa
     4501 ttgcctcaac agtgaattca gaatcgaggt catcccactt gtcaaaagaa gcgccctcaa
     4561 gctgaggggc gtttttcttc aggtttgcca gaacagcaac acgaaaattt ctcatcgaga
     4621 ataaacaact ccgaataaag taatgatatg aacgccgcaa ttataacatc gcagcgcaca
     4681 aaaaaagcgc ggtctaccca gggatttcag gcagaccgcg cggaaaatga tacttacgat
     4741 tcaaccgcaa tcattttggc atcttcttct tccgcccggg caatgccgcg ctccacgttc
     4801 acgccgcgca gcatcaggat ccctgcccca aagaagacga cgagcgagac gatcccccag
     4861 cggccgctgc cggtaagttg gctgatgacc gccatggtag cgggtccgat gaccgtatta
     4921 aatttctccg agatgctgaa aaagccgtaa aattctgcgc tcttgctctt gggcatcaat
     4981 ttgcccacca gcgagcggct gagggcctga ctgccgccct gcaccatggc taccccaaag
     5041 cccaacacgt agaaatgcca ttccttggtc atgaagtacc cggcaatcgc gacgacagag
     5101 taaatcaaca ggctgatggt gatggccttt ttattgccca tctttttggc aagtttgccg
     5161 aacaaaatcg cgaatggcgc ggcgacaaac tgcaccatca ggaaggtgcc cagaatggtg
     5221 atggtgctga agcccaggtc cgtggcgaaa gccgccgcca tggtgataat ggtgccgatg
     5281 ccgttggcgt acaccaggaa agtgagcagg aaccagaata aatctttgaa tttttggatt
     5341 tccttgaatg ctgtcgtaaa tcgggtgata ctgaccttcc agacattctt cccaagctca
     5401 ccgacttccg ccatgcgcgc aggttcctgc acgtggcgga agaggggtat ggagaagact
     5461 gcccaccaaa gcgccacgct ggccaggctc agacgcatcc acaacatggt agcgccgcgt
     5521 tcgcccaggt tcggtgtaag ttttggtccg aggaaaatca tgaccacgtt caccagcagc
     5581 aggatgccgc cgccgatgta acccatagcg tacccgcgcg aagaaacctg atcgatttca
     5641 tcttcatgcg ccacgtgcgg gaggagtgag tcgtagaata caagtgagcc agcaaagccg
     5701 atagtgccca ggatgtacag tatgcagcac agcagccatt cctccggctt gtcgacaaag
     5761 aagagcagcg cggtgctgag gatgcctaag cccgcgaata aagcaaggaa tttcttcttg
     5821 gagccgcgga agtccgcgat ggcgcccagc accgggctga gcagcgccgc tatcaggctg
     5881 ccaatcgcga ccgtgtatcc ccacagcgct aaagaacctt cggtggcgat gtacgcccca
     5941 aagaagttgg gcaggatggc acccatgaca gaagtggcaa aagcggaatt cgcccagtcg
     6001 tacattgtcc aggctctgac ctgaagtttg tgtaaacgct cgttatccat gctggctcct
     6061 tatcgtagaa tgtctaattt taccggttaa ttcatcattc gtgcatgcga tttcgaaaat
     6121 cctgttctgc gccggccaaa tccatcacgc cttgcgcagg aaaaacctgg tcgatgagcc
     6181 tcagcagctc agcttcttct cccgatgtca gcagcgcccg caattgttct ttaggcagcg
     6241 cgtagggttc gaggtaggct ttaaggaact tgcggaaagg cagcagcgcg ccagcaggat
     6301 ggaaagcttg catcgcttcc agttggtagc ggatgagccc gtagatctcc tcagggctga
     6361 cctcgctccg gtcgcgccag ctgaaaatga aggggtttgc ttttgccgcg cgcccgacca
     6421 tggcggcgtc gcagccggtt tgcgcgaaga tttgatgcgc ccgggtgggg ctggttacgt
     6481 cgccgttgcc aatcaccggc acgggcaggc tggccttcag ctccgcaatc ggctgccagc
     6541 gggccttgcc ggtgtaggcc attttcgcgg tgcgcccgtg cagtgcaatc acgcaggcgc
     6601 cgttctcgac ggcaatttca gcaatctggc ggtagttgag tgagtcctca tcccagccga
     6661 ggcgcagctt ggcggtgagc gggacgggca cggctttgct cagctcctgg aaggcttgcg
     6721 ccactttggc gggcgtgcgc agcatgccgg cgcctgcccc gcgtgaggtt acgctggcgg
     6781 tgcagcagcc caggttgacg tcgaagaagt cgggttggta ttcagaatag atttcggcgg
     6841 cgcaatccgc catgcggaag gggtcgttat ccagcagctg cacgccaaag gggcgctcgg
     6901 cagcgcggaa actgagacgg ctgacctggt agtgtttctg attggcgtaa tccagggtgt
     6961 tgatgaattc ggagatggag agcgcggaac ccaggcggcg ggtaatccag cggaagggcg
     7021 ggtcagaaat gccgtccatt ggggagagga tcagacggcc gtagaccggt atgctgccga
     7081 tgcggaaagc aggttcgggc gcggagcttt cagacatgcg cagcagagag gcgtggaaac
     7141 agcccgctcg agttgcatcg agcagacgtt gagtacgtat cgtggcggag tgggtttggc
     7201 acaataattc cctggtcaga tgatg
//
LOCUS       DAOQAD010000091         7222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_12564_length_7222_cov_7.316032, whole genome shotgun sequence.
ACCESSION   DAOQAD010000091 DAOQAD010000000
VERSION     DAOQAD010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12564_length_7222_cov_7.316032"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..687)
                     /locus_tag="PKL60_07130"
     CDS             complement(208..687)
                     /locus_tag="PKL60_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95942.1"
                     /translation="MKKTMTTLAIVSLVLGVAIFGGVSAVSAQKAETGVLADALAEPT
                     VYRRGWAGNGGAGANCDGTCDGSQDPTQLQQRLQDGSGENCTNPEGCTLQQNHFGWDE
                     DGSGKQFQAGNQGAESCPNGGTCAMDQTQTRQQLRDGSCGAEPGTGAGTRTRGGRSN"
     gene            928..1614
                     /locus_tag="PKL60_07135"
     CDS             928..1614
                     /locus_tag="PKL60_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNW95943.1"
                     /translation="MSLILIVEDEKELNKVLQAYLTRAGYRALSAYTGNEGLRLYEQE
                     KPDLVLLDLNLPGRDGLDLAREMRRTRDVPIIMATARVDEIDRLIGLELGADDYISKP
                     YSPREVVARVKAVLRRGAREPKAKTVLTLGKLSLDTDAHSAALAGKPLELTPTEFGML
                     QLLASQPGRVFTRLQLLEAAQGVSYEGYERSVDVHVKNLRRKLKEADPTGQYIETVFG
                     VGYRASRMES"
     gene            1616..3115
                     /locus_tag="PKL60_07140"
     CDS             1616..3115
                     /locus_tag="PKL60_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW95944.1"
                     /translation="MRTRLLIAFLIVIIAALGTVLVVSNFSANTQVRGYLRWSMNANS
                     MVFAEELKSYYQEHGSWSGVERMFTESGGDSSMLGEGANQTGAGQTQGQGAGQTQGQG
                     AGQTQGQGAGQTQGQSAGQTQGQTAGGALTVVRRNGILADENGKVIYANEPARIGTYL
                     TGLERRAATPLEQEGALIGYLFVPSALLNLPANFEAQLMEKVRQAILTAALVSILAAL
                     LLALVLTNLFVKPILELTDNAESIAAGDFSRRVNVKSYPEAESLGKTLNTMAASLQTS
                     EQKRQALTADIAHELRNPLAVQQAQLEALEDGVFPLDLNALKPIREQNALLSRLVDDL
                     RVLALADAGKLSLNKRSTDLRALCQTALQGFEAAFAARDLQVETHCESALPLVNADPD
                     RILQIFHNLLMNAQRYTRQGTKIDISIRQEGAFARVDVRDHGSGIDPEDLPHIFDRFY
                     RSDPGRERESGGTGLGLAIARNLAEAHGGSLQAENHPQGGALFSLRLPL"
     gene            complement(3124..4272)
                     /locus_tag="PKL60_07145"
     CDS             complement(3124..4272)
                     /locus_tag="PKL60_07145"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HNW95945.1"
                     /translation="MSARGTMFDLLKCLGYELKNEQDRSRFIDLATSRLSVLKFGPWE
                     YLSYRSMQGPEVYFGKGAQNEDGSFNAHFHSFTSQDLLLTERIPDPESAPDGLFKALT
                     ASGESLDFEEQNSRGEGQKSTRDSMPLVFKPPNYHWYDHLTLPARARVQLAGFPNEVL
                     LSPPGGAFPMTGKESERQMRSYASPIGVQLVEKGLYHPVLEFGGIISALSVYANELSK
                     KLVYVATVQTVGLNLDLLIPAGMTDLTPAPGMLLKTTCWLTGRIKEVWPAEPQADAPS
                     WVFLLQTRVAGTFFHDLGAKALAIGFWDYLTLQREPENPHDPNAVAILTPDGDMLGYI
                     PRICNQEIAKLLDKGETMHARLIYKFLQDATQQYIIRVYTRSSVRLEN"
     gene            4908..6065
                     /locus_tag="PKL60_07150"
     CDS             4908..6065
                     /locus_tag="PKL60_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNW95946.1"
                     /translation="MKKKTLIVILVLAAAVLIGGGLFLANRWNKPMAEAINLPSPTPS
                     AQSSTPDAQAASVVPTQTSAAATELPTTAFTATAPKPLCGGPDVLYIIAAGIDTDDPN
                     YTYGLSDVMRVIRVDFVTPKVTVLTLPRDLKVELVDIIESKAAELPNAKLNQAYFYGS
                     PGMSYYTGTGGGMGLLARVLAHNYDLYVDNYAAVNMVAFEEIIDAMGGVDVYLEEPID
                     GRPLGKDEVNPYKQGQGYFPKGWNHLTGFDALSFVRIRDRYGEVARTDHQTLLICGIQ
                     DKLTSPEIITSLPKMVLSVLDDIQTDLTLAQIRQLICLLPKLSRENLQFVRFPDEWMV
                     QGRADIARLGNTFVWDIPVEKIVEFINAFETDTIPVVVEEGGTTCPVNPPK"
     gene            complement(6082..7023)
                     /gene="msrP"
                     /locus_tag="PKL60_07155"
     CDS             complement(6082..7023)
                     /gene="msrP"
                     /locus_tag="PKL60_07155"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNW95947.1"
                     /translation="MNPSQTMTQAAETPKPSGDPITPQRLYLTRREFLKAAGIVSASA
                     FLAACLGKPQESAPVNPALVDEQTKRELAENYNNYYEFSLSKTSVAEAARDFRLRPWQ
                     VEVDGLVKNPKTYSLEDILARFPQEERVYRMRCVEGWSMVLPWQGFPLSKLLDEVEPL
                     PEAGYVGFLSVLRPEEMPGQVSLDFPWPYLEGLRLDEARHDLTILASGLYGEALSPQN
                     GGPLRLVVPWKYGFKSLKAVVRITLQADQPVTFWNAIAPQEYGFYANVNPSVDHPRWT
                     QSEERRIGETSARPTLLFNGYDEVAPLYKGMDLVVNF"
BASE COUNT         1495 a         2032 c         2159 g         1536 t
ORIGIN      
        1 cggttcactt gcttgccgtc ctcaagcgta acgaccagat agtaaggcgc gcgcccgaag
       61 tgctggctga tgctcttgcc gttctctgtt atcaaagcaa tcttcatcat attctccatt
      121 tcggccgcaa ttatagtcca ttccaataca aaaccagcac gcgcagggtt gcgcgtgctg
      181 gttgatctta tagaacaggc tgattgctta gttgctccgg cccccacggg tccgggttcc
      241 ggcgccagtg ccgggttccg cgccgcagct gccgtcgcgc agctgttggc gggtctgggt
      301 ttggtccatc gcgcaggtgc cgccgttcgg gcaggattcc gcgccttggt tgccggcctg
      361 gaactgtttg ccagagccgt cttcatccca accaaagtgg ttctgttgaa gcgtgcagcc
      421 ttcggggttt gtgcagtttt cgccgctgcc gtcctgcagg cgctgctgta attgggtcgg
      481 gtcctggctg ccatcgcacg tgccgtcgca gttagcgcca gcaccgccgt ttccagccca
      541 gcctctgcga tagaccgttg gctccgccaa cgcatccgcc aagacgccgg tctcagcttt
      601 ctgcgcggag acggcgctca cccctccaaa aattgccaca cccagtacca ggctcacgat
      661 tgcgagggtt gtcatggtct ttttcatagt tcactccttc atataggttc ggctggaagt
      721 tatccgccgt tgatggtcaa actatatcct ggcgttgttt gcagggggtg aaggggatgt
      781 gaagaactga tgaagattgg ctctttaggg gcgcgcggta caggctgggc gatgaaaaaa
      841 acgggaagat tgggtacact atccctatag aatatctacc agccatctca ccccatgggc
      901 ggctgccagt ttcaggattt gaatacaatg tccctgatcc ttattgtcga agatgaaaaa
      961 gaactgaaca aagtactgca agcttatctt acgcgagccg gctaccgcgc cctaagcgct
     1021 tataccggga atgaggggct gcgcctgtac gagcaggaaa aacccgacct ggtgctgctg
     1081 gacctgaacc tgccgggtcg ggatgggctg gaccttgccc gggaaatgcg caggacccgg
     1141 gacgtcccga ttatcatggc gaccgcccgg gtggatgaga ttgaccggct aatcggcttg
     1201 gaactgggcg cggatgatta catctccaaa ccctactccc cgcgtgaggt agtcgcgcgc
     1261 gtgaaggctg ttttgcgccg cggagcgcgg gagccgaaag ccaaaaccgt cctgacgctc
     1321 gggaagctga gcctggatac cgacgcgcac agcgccgcgc tggctggcaa gccgctggag
     1381 ctgactccaa ccgaatttgg tatgctgcag ctgctggcct cccaaccggg ccgggttttt
     1441 acgcggctgc agctcttgga ggcagcccag ggcgtcagtt acgaaggcta cgagcgcagc
     1501 gtggacgtgc acgtcaagaa cctgcgccga aaactaaaag aagcggaccc caccgggcag
     1561 tatatcgaga cagtttttgg ggttggttac cgcgccagcc ggatggagag ttaggatgcg
     1621 cacgcgcctg ctgattgctt ttctgattgt gattatcgcc gcgctgggga cggtattggt
     1681 ggtttccaac ttttctgcca acacacaggt gcggggatat cttcgctgga gcatgaacgc
     1741 gaacagcatg gtattcgcgg aagagctcaa gagctactac caggaacacg gctcgtggtc
     1801 cggtgtggag aggatgttca cggaatcggg cggagatagc agcatgctgg gggagggcgc
     1861 caatcagaca ggcgccggcc agacgcaggg ccagggcgcc ggtcagacgc agggccaggg
     1921 cgccggccag acgcagggcc agggcgccgg ccagacgcaa ggccagagcg ctggtcagac
     1981 gcagggccaa acggcggggg gcgcacttac agtggtccgt agaaacggga ttctcgcgga
     2041 tgaaaatggg aaagtgattt acgcgaacga gcccgcccgg atcgggactt atcttacagg
     2101 gcttgagcgc cgcgctgcca caccgctgga acaggagggc gcgctgattg gttacctgtt
     2161 cgtacccagc gcgctcttga acctgccggc gaactttgaa gcccaactga tggagaaagt
     2221 ccggcaggcg attctgacgg ctgcgttagt ttccatcctg gccgcgcttt tgctggcgct
     2281 tgtgctgacg aatttgttcg tcaaacccat cctggaattg acagacaacg cggaaagcat
     2341 tgctgccggg gattttagcc ggcgtgtaaa cgtcaaaagc tatcctgaag cggagagtct
     2401 tggcaaaacc ttgaatacaa tggcagcctc gctgcaaact tccgagcaaa aacgccaggc
     2461 gcttaccgct gatatcgccc acgaactgcg caacccgctg gctgtgcagc aggcgcagct
     2521 ggaagctctg gaggacggcg tgtttccgct cgacctcaac gctttgaagc ctattcgaga
     2581 gcagaacgcg ctgctgagcc gcctggtgga cgacttgcgc gtgcttgcgc ttgcggacgc
     2641 gggcaaactg agcctgaata aacgctccac ggacctgcgc gcgctttgtc aaacagcgct
     2701 gcagggattc gaagctgcct ttgccgccag ggacctgcag gtggagaccc actgcgagag
     2761 cgcgctgccg ctggtgaacg ctgacccgga cagaatcctg cagattttcc acaacctgct
     2821 gatgaacgcg cagcgctaca cccggcaggg cacgaaaatt gatatcagca tccggcagga
     2881 aggcgcgttc gcgcgagtgg atgtgcgcga tcacggcagc ggcatcgacc cggaggacct
     2941 gccgcatata tttgaccgct tctatcgctc ggaccccggc cgcgaacggg aaagcggcgg
     3001 cacgggcttg ggtctggcga ttgcccgcaa cctggctgag gcgcacggcg gcagcttgca
     3061 agcagaaaac cacccgcaag gcggcgcgct cttcagcctg cgcctgcccc tttgaaccgc
     3121 ggattagttt tccaaacgga cggaactgcg cgtgtagacc cggatgatgt actgctgggt
     3181 tgcatcctgc aggaacttat aaatcaggcg ggcgtgcatt gtttcgcctt tgtccaacag
     3241 tttggcaatc tcctggttgc agatgcgggg gatgtagccg agcatgtccc cgtcaggggt
     3301 caggattgcc accgcatttg gatcgtgcgg gttttccggc tccctttgaa gcgtgaggta
     3361 atcccagaag ccgatggcga gggctttcgc gcccaggtca tgaaagaacg tgcccgcaac
     3421 ccgcgtttgc agcaagaaca cccaggaggg cgcgtccgct tgcggttccg caggccagac
     3481 ctctttgatt cggccggtga gccagcaggt ggttttcagc agcattccgg gggcgggcgt
     3541 caggtcggtc atgccggcgg gaatcaacaa atccaggttc aagccaacag tttggacggt
     3601 cgccacgtag accagttttt tgctgagctc gttggcgtaa acactgaggg cgctgatgat
     3661 gccgccaaat tcgaggacgg ggtggtacag gcctttttct acaagttgga cgccgattgg
     3721 cgaagcgtaa gagcgcatct ggcgctcgct ctctttgccc gtcatcggga aagccccgcc
     3781 cggcgggctg agcaggactt cattcggaaa gcccgccagc tgcacccggg cgcgcgccgg
     3841 cagcgtcaga tggtcatacc aatgataatt gggaggctta aacaccagcg gcatgctgtc
     3901 gcgcgtggat ttttggcctt cgcccctgct gttttgctcc tcaaagtcga ggctctcacc
     3961 gctggcggtc agcgccttga acagtccgtc gggcgctgat tcggggtcgg ggatgcgctc
     4021 ggtcaggagc aaatcctgcg aggtaaagga atgaaagtgg gcgttgaagc tgccgtcctc
     4081 gttctgcgct cccttcccga aatagacttc cggaccttgc atactgcggt aggaaaggta
     4141 ttcccagggt ccgaatttga gcacggaaag ccggctggtc gccaggtcga taaagcgcga
     4201 ccggtcctgc tcatttttga gttcgtagcc cagacatttc agcaaatcaa acatcgtccc
     4261 tctggcgctc attatacatt acgctccgga gagttttcag cgcaactggc gttccacggg
     4321 gcggaggaag gccgttggaa agaccctcat taacctcctg gcgttggttc gcttctccag
     4381 ggagaagctc agcattccgg ccgcgctcag ggaagaggcc cgtccagtca gggcgaaatg
     4441 gaaacgtcct gatcagtgga tgaagtcgtc gggacggatt tttccccgaa actaagcccc
     4501 tgatttcttc cttccgatca tgtttcaggc ttgccctacc ccagacccat ccccagcagc
     4561 aaacggtttt ttttgcctcc gtcaagccgc tggagaaggg aaacacgatt gattgaatca
     4621 atgatcaact ccccgtccca gcgattgttc agcaggagaa caaaataaat tactttcggt
     4681 gaagaagggg aggaagaggg gaaaggctgc tctaccattg tgtgtcagga tatgatcctt
     4741 cctttggtaa aacttgcgga aatcgaataa tgatttcctg gctcctgatc ataatcagga
     4801 catttctgtt ttgctatttc aggataagcc tgtcaaagag cgtcttgaga atttacgcta
     4861 taatacacag cgattgttct cactaaggca gtctgtggaa tatggatatg aaaaaaaaga
     4921 ctttgattgt gattcttgtc cttgccgccg cggtgctgat tggcggcggg ttattcctgg
     4981 caaaccgctg gaataaacct atggcagaag ccattaatct gccttccccc acaccctccg
     5041 cgcagagcag cacccccgac gcgcaagccg caagcgttgt tcctacccaa acaagcgcgg
     5101 ctgccaccga actgccaacc acggctttta cggcgaccgc cccaaagccc ttgtgcggcg
     5161 gaccggatgt gttgtatatc atcgcggctg ggattgacac cgacgacccc aactacactt
     5221 acggtctctc ggatgttatg cgcgttatcc gcgtggattt tgttaccccc aaggtgactg
     5281 tcctcaccct cccgcgcgac ctgaaagtgg aacttgtcga cattatcgaa tccaaagcgg
     5341 ctgagttgcc gaatgccaag ctgaaccagg cttattttta cggcagcccg ggcatgagct
     5401 attacaccgg caccggcggc ggtatggggc tgctggcgcg cgttctggcg cacaactacg
     5461 acctgtacgt ggataactat gccgctgtaa atatggttgc cttcgaggaa atcattgacg
     5521 cgatgggcgg cgtggacgtg tatctggaag aacccatcga cggcagaccg ctgggaaagg
     5581 atgaagttaa cccctacaaa caaggccagg gttactttcc caagggttgg aaccacctga
     5641 ccggtttcga cgccctcagt tttgtgcgca ttcgcgaccg ctacggcgaa gtggcgcgca
     5701 ctgaccacca gaccctgttg atttgcggca tccaggataa gctgaccagt ccggaaatta
     5761 ttaccagctt gcccaaaatg gtgctttcgg tgctggatga tattcagacc gacctgaccc
     5821 tggctcaaat ccgtcagctc atctgtctgc tgcccaagct ctcacgcgag aacctgcagt
     5881 tcgtgcgctt tccggatgag tggatggtgc aagggcgcgc ggatatcgcg cggcttggca
     5941 atacctttgt ctgggatatc ccggtggaaa aaatcgtgga gtttatcaat gccttcgaga
     6001 ctgataccat ccccgtggtg gttgaagaag gtgggacgac ctgcccggtg aatccgccaa
     6061 agtaatcagc gaagcttact cctaaaaatt cacgacgagg tccatgccct tgtaaagcgg
     6121 tgcgacctcg tcgtatccgt taaagagcag cgttggtctg gcgctggtct cgccgatacg
     6181 gcgctcttcg ctctgcgtcc agcgcggatg gtccacggaa gggttgacgt tggcgtaaaa
     6241 gccgtattcc tgcggggcta tcgcgttcca gaatgtgacc ggttggtcag cttgtaaggt
     6301 gatgcgcacc acagccttca ggcttttgaa gccgtacttc cagggaacca ccagacgcag
     6361 cgggccgccg ttctggggcg agagcgcttc gccataaagg ccgcttgcca ggatggtcag
     6421 gtcgtggcgg gcttcatcca ggcgcagccc ttccaggtaa ggccagggaa aatcaagact
     6481 gacctgaccg ggcatctcct ccgggcgcag taccgacaga aaacccacgt acccggcttc
     6541 cggcaggggc tccacttcat ccaacagctt ggacagggga aaaccctgcc agggcagcac
     6601 catcgaccaa ccttccacgc agcgcatgcg gtaaacgcgt tcttcctgcg gaaagcgggc
     6661 gaggatatcc tcgaggctgt acgttttggg gttcttcacc agtccatcga cctcaacctg
     6721 ccaggggcgt aacctgaaat cccgcgccgc ttccgccacg gaagttttgg agaggctgaa
     6781 ttcgtaataa ttgttgtaat tttccgccag ctcgcgtttt gtctgttcat ccaccagggc
     6841 agggttgaca ggcgcgcttt cctgcggctt tcccaggcag gcggccagaa aggcgctcgc
     6901 gctgacgatg cccgccgctt tcaggaactc ccggcgggtg agatagagcc tttgcggcgt
     6961 gatggggtcg ccagaaggtt tgggggtttc tgctgcctgg gtcatggtct gactcggatt
     7021 cacctttctt ttcgatattt tataaaatcc gccgcggaat aacaataaaa agcttattca
     7081 aagcaggatt tcaatgcggg ggcggctttc agccgaagcc gcgctttgat ggttgggcgg
     7141 ttagaggagc catctgcttc ctgcctcctt tggacctgcg cataggcgtc aatccgaagg
     7201 gaagtgccgg cggattgcct gg
//
LOCUS       DAOQAD010000092         6960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_13234_length_6960_cov_5.455467, whole genome shotgun sequence.
ACCESSION   DAOQAD010000092 DAOQAD010000000
VERSION     DAOQAD010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13234_length_6960_cov_5.455467"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="PKL60_07160"
     CDS             complement(<1..1210)
                     /locus_tag="PKL60_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95948.1"
                     /translation="MAPLPAAIWILGAVLTGGALFGLWKSLRPPLNPGKKEIRWLALL
                     AILLLPCNYFARFTPFRGFFISFDGSQFPAQTSLLALIPAFTALGFVGPPAAVALAVV
                     SGLSQWLLFGQDFSVVLQFAVLTVLFITLSRRTEMRASGQSRYKPLINALLAYLLALP
                     VMLVVRLVVSLSYGQNDLMTILEQFVLVSAVLLPSALVGGAACELLQRWLGSEWNPMD
                     YLAEWEVHSPIKQTIAQIERLTLGRYDRAINFEVHNPAEAKLAKALDDLRENLRLRND
                     TQTRLLSLDPSHYSREGYDLVLSSILRAALGRDASTARLVLLSPGFGDQQVEMRLRLG
                     QGEQTRVYAYLDAMILDKLGSQDQLILSDLKVDQYFGLSSGMPYPQSIAALRLKSEDV
                     TQGILWVGFEQ"
     gene            complement(1313..2014)
                     /gene="cmk"
                     /locus_tag="PKL60_07165"
     CDS             complement(1313..2014)
                     /gene="cmk"
                     /locus_tag="PKL60_07165"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNW95949.1"
                     /translation="MATEAPQIIAIDGPAASGKSTVGAALAEKLGYLCLDTGIMYRAV
                     TWLALAKGLDPQDETAITRLAETIEIDVQPGSVSDGRGFDVLINHEDHTWDIRLSEVN
                     QHVSLISSYPGVRAAMTLQQRKIASRGKIVMLGRDIGTVVLPDAGLKIYLDASLKVRA
                     RRRYEEEKARGSSITYQQVVASLRRRDKIDSSRKYAPLKIADDAVVINTEALSAAQVV
                     ERILALIESPAAPVP"
     gene            complement(2029..3750)
                     /locus_tag="PKL60_07170"
     CDS             complement(2029..3750)
                     /locus_tag="PKL60_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95950.1"
                     /translation="MPEEIFVGGSKRWYADARLIILAIFFAGLCLRLNQLGSVPFSSE
                     EAQNACSAFSVAKGVPVGTAASPAYEGLTSLTFFLLRASPFFARLWPALAGSSLALAP
                     LFFRRRVGSLQAVLLAAAFAFDPVLFTLSRLAGGAIFLIVGLIWALIFFVARKPVGLG
                     IALALAWLSGPWLWIILLVSGVGFGLFLLAGKQADLSGLTNACPFRTKAFRETAAVSF
                     AVSLFLLASAFLLNPAGLSGIAGGLARLWPSSHEMTKTALTLPIYRLIAYSLPVLILA
                     FLASLSAWREKERWGQLVSLLAGITLLAILLFRNQGTAGYALLQFLLWLQAVRTLEKQ
                     VQLEAGRRDIALGAFLLGIALCGYLALSAKNLVNPTQAGLTFGPGAIAFVLGLLLLVF
                     VFFLVMMGWSLQTARKGILSAVVLSLLAFSLSLTFLALKPSDPYSALVWSESNVWVNA
                     DAQSLLLREIEKSGKLEAQVSRVVITDPQLADLRWDFRDYGRVEVLSVLPENYPPEIL
                     ITAAPDQQKAADSYRGIQMFSPGRVPWEKFTPVEILRAAVSKSLPAESFSYYLWIRQD
                     LMTGALP"
     gene            complement(3753..6434)
                     /locus_tag="PKL60_07175"
     CDS             complement(3753..6434)
                     /locus_tag="PKL60_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNW95951.1"
                     /translation="MNLVDLLKWYALAMILGWLNLPLTRRLFKALPSRGFALSRPIGL
                     LLWGYSFWLLTSLKLLNNDLAGQLTALLLLAVLNLALLGRGGIKELADWVRRNRKLIL
                     CVEGLFLFAFALWAFVRAANPAILGTEKPMEMAFINAILRSPSFPPNDPWLSGYAISY
                     YYFGYVLAAMLIRVTGSLSAVGYNLVAALWFALTAAAAYGLLFDLLAARHKNSAKKPV
                     WIYTLSLLAPALLLIVSNWHGILDVMHARGLFYTTAADGAFSSTFWDWLKLKEISGVN
                     PDFSWFPLRWGGVQWWGASRVVQDFQLNGNALEVIDEFPFFSYLLSDIHPHVLGMPFV
                     LAAVGQALNAILGGWEGKTRIWRWALPWSLPEMLLAVLSLGGIAFLNTWDFPFYLALI
                     AAALVYRRWLAYGWSGKRIIEFLLLCFGMAVLAIAAYLPFYLSFSSQAGGILPSLAFF
                     TLGRHFWIMFGPLLVPILLGLGWQLQRNREKHGFLTAFWITCALFAALFVFSWGIAFM
                     STRLPELGQFILSLQGPAVGQNLLSGALIARLKAPGTGITLFLLLFLGLSALIKPGSS
                     SSMEESPVRELPERTVDVFVIFLVLLGGLLTLAPEFVYLRDQFGTRMNTIFKFYFQAW
                     ILWSAAGAYFTARFFEARGSARAWERLIPALMLLLGTAAAFLGLYRADDTFLRFNQNL
                     GAFGSAPLDFIIAGIFVLGFLWAFVELLTGRKTAALAALCVCAFAAGLVYPVVSTWNK
                     LGGSPSLEQLTLDGSQFYRDTWADQMQAVDWLQQQPLGVMVEAVADTGGSYTTYASVS
                     TFSGMPTVLGWIGHVAQWRGGYEEMGTRQEDVRVLYSTHDWDEARAVLDKYNIAYVYV
                     GELEARTYALDEAKFAEHLSLGYDSANARVYLYAPEK"
     gene            complement(6431..>6960)
                     /locus_tag="PKL60_07180"
     CDS             complement(6431..>6960)
                     /locus_tag="PKL60_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95952.1"
                     /translation="PLLGTTDKINDRMNPAAPKTLDGMEYMQSSNYYDMGVDMPLEDD
                     YFCIRWMQDHVSGSPVIVEAQAYEYRWGNRYTIYTGLPGVVGWNYHQRQQRAILQNNA
                     VQNRVDEVNQFYLTNDLDFVREFLEKYQVSYIIVGRLEKAFYPGGGLEKFEAQDGILW
                     DQVFQYDSTLIYKVR"
BASE COUNT         1725 a         2057 c         1873 g         1305 t
ORIGIN      
        1 tctgttcaaa accaacccac aggataccct gagtgacgtc ttcgcttttc aggcgcagcg
       61 cggcgatgga ctgcgggtac ggcatgccgg aagaaaggcc aaagtactgg tccaccttca
      121 ggtcgctcaa aatcagttga tcctggcttc cgagcttatc gagaatcatc gcgtccaggt
      181 acgcgtatac ccgggtttgt tcgccctgac caaggcgcag gcgcatttca acctgctgat
      241 cgccaaatcc cggactaagc aaaaccaatc gcgcagtgga agcatccctg cccaaggcgg
      301 cgcgcaggat ggaagagagc accaggtcat atccctcgcg ggaataatgc gaggggtcca
      361 gagaaagcag gcgggtttgc gtgtcattgc gcaggcgcag attttcgcgc aggtcatcca
      421 gcgcttttgc gagttttgct tcagccgggt tatgaacttc gaagttaatc gcccggtcgt
      481 agcgccctaa agtcagccgc tcaatttgcg cgatagtttg ctttatcggg ctgtgtactt
      541 cccactccgc caggtaatcc atcgggttcc attcgctgcc cagccagcgc tgcagcaatt
      601 cgcaggcagc gccgccaacc agcgcggacg gaagcagcac agcgctgacg agcacaaact
      661 gctccagaat ggtcatcaaa tcattctgac catagctcaa ggagaccacc agacgcacaa
      721 ccagcatcac cgggagcgcc agcaggtacg caagcagcgc gttaatcagg ggcttgtacc
      781 tgctttgccc gctggcgcgc atctccgtcc gccgtgagag cgtgatgaac agcaccgtca
      841 ggacggcgaa ctgcagtacc acgctgaaat cctgaccgaa taagagccat tgcgaaagcc
      901 ctgaaactac tgccagcgcc acggcagccg gcggacccac aaagccgagc gctgtgaaag
      961 ccgggatgag cgcgagcagg gaggtctgtg cagggaattg acttccgtca aaacttatga
     1021 aaaagccacg aaagggtgta aatcgcgcga aataattgca tggaagcagc aaaattgcca
     1081 gcagcgccag ccagcggatt tctttctttc cgggattaag aggaggtctc aggctcttcc
     1141 aaagtccaaa gagggcgccg cctgtaagga cagcgccaag tatccatatc gccgcaggca
     1201 gcggtgccaa tggagacggc gctaatagaa ctgctgatac gtgaaaaaaa acggtcatat
     1261 cccaaggaat attatagcac ctcccccagc agaaaaaacc ggtaaacccg ggttagggaa
     1321 caggcgctgc cggcgattct atcagtgcga ggatgcgttc aacaacctgc gcagcgctta
     1381 gagcttctgt gttaatgacc accgcgtcat ccgcaatttt caacggagca tatttccgcg
     1441 aggaatcgat tttgtcccgc ctgcgcagtg aagccaccac ttgttggtag gtaatcgagc
     1501 tgccgcgggc cttttcctcc tcatacctcc gcctggcgcg gactttcaga gaggcatcca
     1561 ggtaaatttt tagtccggca tccggtaaga ccaccgtgcc gatatccctg cccagcataa
     1621 cgatcttgcc cctggaggca attttccgtt gttgcaaggt catcgccgcc cgtactcccg
     1681 gataggagga aatgagcgaa acgtgctgat tgacctccga aaggcggata tcccaggtgt
     1741 ggtcctcgtg attgatgagc acgtcaaacc cgcggccgtc tgagacactg cccggctgca
     1801 cgtcaatttc gatggtctcg gccagccgcg tgatcgcagt ctcatcctgc ggatcaaggc
     1861 ccttggctaa cgccaaccag gtgaccgcgc ggtacatgat gcccgtatcc aggcaaagat
     1921 aacccagctt ttctgccagc gccgcaccta ccgtggactt tccgctcgcg gctggaccgt
     1981 caatcgcgat gatttgtggg gcttctgtag ccatctcttt tcctggcatt atggtaaagc
     2041 tcctgtcatc agatcctgac ggatccagag ataataactg aaactttcag cgggcaggct
     2101 cttgcttaca gccgctctga gaatttccac cggtgtaaat ttttcccatg gtactcggcc
     2161 ggggctaaac atttggattc cgcggtaact atccgccgcc ttctgttgat cgggtgcagc
     2221 cgtaatcagg atttctggcg ggtaattttc agggagtacg gataaaacct ctacccggcc
     2281 gtaatcccgg aaatcccaac gtaaatccgc cagctgcggg tccgtgatga caactctcga
     2341 aacctgtgct tccagtttgc cggacttttc gatttccctg agcagtaaac tttgcgcgtc
     2401 cgcattcacc cacacgttag actctgacca gaccagggcg gaataggggt cggatggttt
     2461 taacgccaga aaagtgagac tgaggctgaa cgcgaggagg ctgagcacta cagcgctgag
     2521 aatccctttg cgcgcggtct gcaaggacca acccatcata accaggaaaa acacgaaaac
     2581 taacagcagt aaacccagta cgaaagcaat cgcgccgggg ccgaaagtta atccggcttg
     2641 cgtcggattt accaggttct tcgcgctaag cgccagatat ccgcagagcg ctatccccag
     2701 caagaatgcg cccaaggcaa tatctcgtcg tcccgcttcc agttggacct gcttttcgag
     2761 cgttcgcacc gcctgcagcc ataataaaaa ctgcagcagc gcgtatccgg ctgttccctg
     2821 gtttcggaaa agcaaaatcg caaggagcgt gattcccgcg agcaggctga caagctgccc
     2881 ccatcgctct ttctctctcc aggcggaaag tgaagcaaga aaagccagga ttaagaccgg
     2941 taagctatac gcaatcagcc tgtatattgg gagtgttagc gcagttttgg tcatttcatg
     3001 gctggatggc cataagcgag ccaatccgcc ggcaatccca ctgagacccg caggatttag
     3061 cagaaacgcg ctggccagga ggaagaggct gacagcaaaa ctcaccgcgg cagtttcccg
     3121 aaaagctttg gtccggaaag ggcaggcgtt tgtaaggccg cttaggtctg cctgcttgcc
     3181 tgccaacagg aaaagcccaa aacctacgcc actaaccagc aggattatcc agagccaggg
     3241 cccgcttaac caggccagcg ccagagcaat ccccaacccg acaggcttgc gcgcgacaaa
     3301 gaagatgagc gcccagatta acccgacgat cagaaagatg gctcccccag ccagtcgtga
     3361 gagcgtgaac aagacagggt caaaggcgaa tgccgcagct aacaaaactg cctggagcga
     3421 acctacccga cggcggaaaa agagcggcgc aagcgccagg ctggagcccg ccagcgcggg
     3481 ccacagacgc gcgaagaacg gcgaagcccg cagcaggaaa aaggtcaggg aggtcaagcc
     3541 ctcgtaagcc gggctggcgg ctgtgccaac gggaacacct ttcgccacag agaacgcaga
     3601 gcaggcgttt tgcgcttctt cagagctgaa ggggacgctg cccagctggt ttaggcgcag
     3661 gcaaagaccg gcaaagaaaa ttgccagaat tatcagacgc gcgtccgcgt accagcgttt
     3721 tgacccgccg acaaaaattt cttcaggcat gcttatttct ccggtgcgta aaggtatacg
     3781 cgcgcattcg cggaatcata accgaggctg aggtgttcgg caaattttgc ctcgtccaag
     3841 gcgtaagtgc gggcttccaa ttctccaacg taaacatagg caatattata cttgtcgagc
     3901 accgccctgg cttcgtccca gtcgtgcgtg ctgtacaaaa cccgaacgtc ttcctgtcgg
     3961 gtgcccatct cctcatatcc gccgcgccac tgcgccacat gccctatcca tcccagaacg
     4021 gtgggcatcc cggaaaaagt ggaaacggat gcgtaagtcg tatagctgcc gccggtatcc
     4081 gctaccgctt ccaccatcac ccccaggggc tgctgctgca accaatccac agcctgcatc
     4141 tggtccgccc aggtatcccg gtagaactgc gagccgtcca gcgtgagctg ctccaaggaa
     4201 ggcgagccgc ccaacttatt ccaggtggac acgaccggat acaccagccc agccgcgaac
     4261 gcgcatacgc acaatgccgc caacgcagcc gttttgcggc ctgtgaggag ctccacaaac
     4321 gcccataaga atcccagaac gaagattcca gcgataataa aatctagcgg cgcgcttccg
     4381 aaagcgccca ggttttgatt gaagcgcagg aaggtatcat ctgcccgata cagccccaaa
     4441 aaggcggctg ccgtgccgag caggagcatc agcgccggga ttaaccgctc ccaggccctt
     4501 gcgctgccgc gcgcttcaaa aaaccgtgcc gtgaaatacg ccccagcagc cgaccacagg
     4561 atccaggcct ggaaatagaa cttgaaaatg gtgttcatgc gcgtcccgaa ctggtcgcgc
     4621 agatacacga actccggcgc aagcgtcagc agcccgccca gcagcaccag gaagataaca
     4681 aatacatcca cagtccgttc cggcagctca cgaaccggac tttcctccat cgaagatgag
     4741 cttccgggct ttatcagcgc gctcaagccc agaaaaagca gcagaaacag cgtgatgccg
     4801 gtgccgggcg ctttcagtcg ggcaattaaa gccccagaca gcaggttttg ccctacggcg
     4861 gggccttgca gcgagagaat aaactggccg agttctggca agcgggtgga catgaatgct
     4921 atcccccagg agaacacgaa cagcgccgcg aatagcgcgc aggttatcca gaatgccgtc
     4981 agaaacccgt gcttttctct attacgctgc agctgccagc caagacccaa caggattggg
     5041 accagcagcg gaccaaacat gatccagaaa tgtcttccca gcgtaaagaa cgccaggctg
     5101 ggcaaaattc cgcccgcctg ggaggagaaa ctgaggtaaa agggcagata cgccgcgatt
     5161 gccaggacgg ccatgccaaa acaaagcaat aaaaactcga tgatgcgttt tccgctccag
     5221 ccgtacgcca accaacgccg ataaaccagc gcagccgcaa tcaacgccag atagaatgga
     5281 aagtcccagg tattcaggaa agctatcccg cccaggctga gcaccgccag cagcatttcg
     5341 ggcagactcc acggcagcgc ccagcgccaa attcgggtct ttccttccca gccgcccagg
     5401 atggcgttga gcgcctgccc aacggccgcc aacacgaaag gcatgcccaa cacatgcggg
     5461 tggatatccg aaagcaggta cgaaaagaac gggaattcgt cgatgacttc cagcgcatta
     5521 ccgttcagct gaaaatcttg tacgacgcgg ctggcgcccc accactgcac cccgccccaa
     5581 cgcagcggaa accaggaaaa atccgggttg acaccgctga tttccttcaa tttcagccaa
     5641 tcccaaaaag tggagctgaa agcgccgtcc gctgcagtag tatagaacaa gccgcgggcg
     5701 tgcatgacgt ccaggattcc gtgccagttg gagacaatca acagcagtgc cggcgccaac
     5761 aggctgaggg tataaatcca gacgggcttt ttcgcgctgt ttttgtggcg tgcagccaga
     5821 aggtcgaata gcagtccata ggccgcggcg gcggtcagcg cgaaccacag cgccgccacc
     5881 aggttgtacc caaccgctga taaagaaccc gtcacgcgga tgagcatggc cgccagaacg
     5941 tatccgaagt aataatatga gatagcatac cctgaaagcc aggggtcgtt gggtggaaaa
     6001 gacggggaac gcaagatggc gttgatgaat gccatttcca tcggtttctc agtcccgaga
     6061 atcgcgggat tggcagcgcg cacgaatgcc cacagcgcga aagcgaacag aaacagccct
     6121 tcaacgcata gaatgagttt ccggtttctg cgcacccagt ctgccagttc ctttattcct
     6181 ccgcgcccca gcaaagccaa attaagcacg gcaagcagaa gcagagccgt cagctgaccg
     6241 gccaggtcgt tgttcaacag cttcagcgag gtcagcagcc aaaagctgta tccccacaag
     6301 agcagtccta tcgggcggct gagcgcgaac ccgcggctgg gcagcgcctt gaacaagcgc
     6361 cgggtcaagg gcaggttcag ccaaccgagt atcatcgcca atgcatacca tttgagcaag
     6421 tcgaccaagt tcacctcacc ttataaatta gcgtgctgtc gtattgaaaa acctgatccc
     6481 aaaggatgcc gtcttgcgct tcgaattttt ccagtccgcc gccggggtag aaagcttttt
     6541 ccaaccggcc aacgatgatg taactgacct gatatttttc cagaaattct ctcacaaaat
     6601 caaggtcatt agtgagataa aactggttga cctcatccac gcggttctgt accgcgttat
     6661 tctgcaaaat tgcgcgctgc tggcgttggt ggtaattcca cccgaccacc ccgggcaggc
     6721 cggtatagat cgtataacgg ttgccccagc ggtattcgta cgcctgggct tccacgataa
     6781 ccggcgaccc gctgacatga tcctgcatcc agcggatgca gaagtaatca tcctccagcg
     6841 gcatgtccac gcccatatcg taatagttgg agctctgcat gtactccatt ccgtccagcg
     6901 ttttcggagc ggctgggttc atgcggtcgt ttatcttatc cgtggtgccc agcagcggga
//
LOCUS       DAOQAD010000093         6889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_13431_length_6889_cov_5.536435, whole genome shotgun sequence.
ACCESSION   DAOQAD010000093 DAOQAD010000000
VERSION     DAOQAD010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13431_length_6889_cov_5.536435"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1327
                     /locus_tag="PKL60_07185"
     CDS             107..1327
                     /locus_tag="PKL60_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNW95953.1"
                     /translation="MHTSWDHRYAHRTAGMTSSFIRELLKVTAQPDVISFGGGFPAAE
                     LFPLDRVKAACDKVLTENGAKALQYSQTEGYAPLRELIAGNVARYGIKVSADNVLITT
                     GSQQVLDFIGRIFINRGDRVLTESPTYLGALQAWNAYGAVYETVISDNDGLSTDDLEK
                     VIGSHIKFMYVLPNFQNPAGVTLSLERRQKLVELADRFCVPIVEDDPYGQLRFEGEHL
                     PPVVVMDDALRDKANLTYRGNVIYTSTVSKILAPGLRIGWVIAPNEVIKKMVQAKQGA
                     DLQCSTFDQYLAYELLQGDWMKDHIQMLRRVYKERRDAMLQAFEDFMPEGTTWTRPQG
                     GLFLWLRLPEGCNATELFPIAVEEKVAYVPGEPFYPNGGPLNTMRLNFSACNPETIRV
                     GVERLSRMVKRCIK"
     gene            complement(1499..3211)
                     /locus_tag="PKL60_07190"
     CDS             complement(1499..3211)
                     /locus_tag="PKL60_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNW95954.1"
                     /translation="MEHNEFYLSDKARETVELSLPDGRIITGRRGAALMEFLRVLEKS
                     QEAMIVGAIVDGNLRELTFPIRRDALVTPLTLRDADGARIYRRSLTFLMEVAFKQCFP
                     GGVLTIDHSVSSGGYYCQVENVPDFSRADLNRLEAAMRELVRQDVAFIRETVPLQEAI
                     DYFLRAGEDDKVRLLRYRKRNDLVLYRVSETRDYHHGYMVPSSGYLKWFGLEMLNGAF
                     ILRFPRRHAPSELLPMASYPTLLKTFRDYGDLLKKLGIPSVGALNDAIEAGHIDEVIL
                     VSEALHEMQISEIAEAIDKRKDSVGVILIAGPSSSGKTTFSKRLSIQLLARGITPFAM
                     EMDNFFVDREKTPLGKDGKPDFESLHAMNLERLYADVRGLMDGKEVQLPRFDFKTGRS
                     LEGERAKLNKGDIIILEGIHGLNPGLMPHLKRENTFRVYVSCLTQLNLDRHNRISTTD
                     TRMLRRILRDARDRGYSALDTIQRWESVRQGEKVHIFPYQEHADAIFNSALAYELTTL
                     KPLVEPLLRQVPFGTPEYIEAKRLLKFLEWFLPTGTEYVPGNSILREFIGGSSLTEFS
                     LWKK"
     gene            complement(3468..5162)
                     /locus_tag="PKL60_07195"
     CDS             complement(3468..5162)
                     /locus_tag="PKL60_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNW95955.1"
                     /translation="MKQHKFVSGVLAALLFLLAACQPAPTAAPALTPPAESTTSAAPA
                     LTPTAESTPEELSGWWNDVVFYEIFVRSFKDSDGDGIGDFQGIIQQLDYLNDGDPATN
                     TDLGVGALWLMPINPSPSYHGYDVTDYKAVNPDYGTLDDFRQLLEACHARGIRVIMDF
                     VVNHTSTQHPWFKAALAGDPKYKDYYVWSDSDPSSLGPWGQRAWYRASNGKFYYAVFW
                     DGMPDLNYHNPAVTEEIQSASAFWLDLGVDGFRVDAARYLFEEGAALQDTKETIQWFQ
                     DWRAFTKPLNPQAYTVGEVWTDLQITAKYGHPKGLDSLFMFDLAEDIKGAAYSGDASL
                     ALKSYLDVLAYFPDLQFSTFLSNHDQQRPMSYFGGKESKARVAAFIYLTGPGTPFVYY
                     GEEIGMTGSKPDENLRTPMQWSTEPDSAFTSGIPWEPVNKGWEETNVETQAANPDSLL
                     NWYKGLIQLRNQHQALRGGVYLPLTSSCRRVYAVLRQSEDDILLTLASTGIPSSENCT
                     IALEGSPLSGSYQAETLWGEPLFDEISFGADGSLKDFLVAPVLNGGQTLIVRLSQP"
     gene            complement(5326..6006)
                     /locus_tag="PKL60_07200"
     CDS             complement(5326..6006)
                     /locus_tag="PKL60_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNW95956.1"
                     /translation="MCGRFSMAIDSDDLQTALALGQMPDDWKRRYNIAPSQPVAALRD
                     PESRQVDWLQWGLVPFWAKDPAIGSSLINARAESVFEKPSFKYAFRKRRCLILADGFY
                     EWQKTGAKGSRPFYFSMRSGEPFAFAGLWETWQPKDQPGEALSTCTIITTAANETVAP
                     VHERMPVLLRKENMWDWLLETSETALVSMLRPAPADWLRAWEVSRAVNSPNQEGPECA
                     APLQSSNG"
     gene            6530..>6889
                     /locus_tag="PKL60_07205"
     CDS             6530..>6889
                     /locus_tag="PKL60_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95957.1"
                     /translation="MRNWFSRLIAVIIILGALSPAALTSAEAQTPPPPPEPERRARCL
                     PGQPCEDDNGERFMLPTEETLAEPAAIGDTDDYGYTITSTTYSWIDATGGTNTGRCRQ
                     RRSTAWQPSMLPRNRYAT"
BASE COUNT         1398 a         2054 c         1996 g         1441 t
ORIGIN      
        1 cgccgaactc accatcggcg ataaggtcgt gcttcccggc gcagccacga ttatcagcag
       61 gtttttgaaa gtctgagtaa tcacaaaaaa aggagagtga aacaatatgc atacatcatg
      121 ggaccatcga tacgctcacc gcaccgctgg gatgaccagc tctttcatcc gtgagttgtt
      181 gaaggtcacc gcccagccgg atgtcatatc ctttggcggc gggtttccgg cagcggaact
      241 ctttccgctg gacagggtca aggcagcctg tgacaaagtg ctcacggaga acggcgccaa
      301 agccctgcag tacagccaga ccgagggcta cgcgccgctg cgcgaactca tcgcgggaaa
      361 cgtcgcccgg tacggcatta aagtaagcgc ggacaacgtc cttatcacca ccggctcaca
      421 acaagtgctc gacttcatcg ggcgaatctt tatcaaccgc ggcgaccgcg tgttgaccga
      481 gtccccgacc tatttgggcg ctttgcaggc ctggaatgct tacggcgcgg tatacgagac
      541 ggtaatctcg gataatgacg gcctgtccac cgatgacctg gaaaaggtga ttggcagcca
      601 tatcaagttc atgtacgtcc tgccgaactt tcaaaacccc gccggcgtta cgctttcgtt
      661 ggaacgccgt cagaagttgg ttgaactcgc ggatcgcttc tgcgtcccga ttgtggaaga
      721 cgacccctac ggacagctgc gatttgaagg agaacacctc ccgcctgtgg tcgtgatgga
      781 cgacgcgctg cgcgataaag ccaacctgac ctaccgcggc aacgtcatct acaccagcac
      841 ggtttcgaaa atccttgccc ccggactgcg catcggctgg gtcatcgctc ccaacgaggt
      901 catcaagaaa atggtgcagg ccaagcaagg tgccgacctg cagtgctcca ccttcgacca
      961 gtacctggcg tacgaactgc tgcagggaga ctggatgaaa gaccacatcc agatgctgcg
     1021 ccgtgtgtac aaagaacggc gcgacgcgat gctgcaggct ttcgaggact tcatgcccga
     1081 aggcaccacc tggacccgcc cgcaaggtgg tctgttcctg tggctgcggc tgcctgaagg
     1141 ctgcaacgcc acggaactct tccccattgc ggtggaagag aaagtagctt atgttcccgg
     1201 cgagcctttc taccccaacg gcggccccct gaacaccatg cgcttgaatt tttccgcttg
     1261 caacccggaa accattcggg ttggcgtgga acgcctctcc cgcatggtca agcgctgcat
     1321 aaagtagacc gggttaaaca caaaccagcg gatttctggg ggaatagatc cgctggttgt
     1381 tttattaagc tggaatttta tccttcccta tttgcaagtt actggtaaat agagcatctt
     1441 gcaaacaacg tctgttcctt gccaataaat aggcgccccg gattttttaa tcttgttcct
     1501 acttcttcca cagcgaaaat tcagtcaggc tcgaaccgcc gataaattcc cgcaggatgg
     1561 agttgcccgg tacgtattcc gtgccggtgg gcaggaacca ttccagaaac ttgagcaggc
     1621 gtttggcttc gatgtactcg ggcgtgccaa agggtacctg gcgcagcagc ggttccacca
     1681 gcggctttag agtggtcaat tcgtatgcca gcgccgaatt gaaaatcgcg tcggcatgtt
     1741 cctggtaagg gaaaatgtgc accttctcgc cttgccgcac ggattcccaa cgttggatgg
     1801 tgtccaacgc ggaatagccg cggtcgcgcg cgtcccgcag gatgcggcgc agcatgcgcg
     1861 tgtccgtggt ggaaatgcgg ttgtgccggt ccaggttcag ctgggtcagg caggagacat
     1921 acacccggaa ggtattctcg cgtttgaggt gcggcatcag cccggggttc agcccatgga
     1981 tgccttccaa gatgataata tcgcctttgt tcagtttggc gcgttcacct tccaggctgc
     2041 gcccggtctt gaagtcaaaa cgcggcagct gcacttcctt gccatccatc aagccgcgca
     2101 cgtctgcgta taagcgctca agattcatcg cgtgcaagga ctcaaaatca ggcttgccgt
     2161 ccttgcccag cggggttttt tcgcggtcta cgaagaaatt atccatctcc atcgcaaagg
     2221 gcgtgatgcc gcgcgccagc aattgaatgc tcagacgctt cgagaaggtc gtcttgcccg
     2281 aggaagacgg accagcgatc aggatgaccc cgacgctgtc cttgcgtttg tcaatcgcct
     2341 cggcgatttc cgaaatctgc atctcgtgca gcgcttctga taccagaatg acctcatcga
     2401 tatgccccgc ctcaatcgcg tcgttcaagg ctcccacgga tggaatcccg agctttttca
     2461 gcaagtcgcc gtaatcgcgg aaagttttca gcagggtcgg gtagctggcc atcggcagca
     2521 gctcggaagg ggcatgccgg cgcgggaagc gcaaaataaa cgccccgttc agcatttcca
     2581 gcccgaacca cttgaggtag ccggaggaag gcaccatata gccgtggtgg tagtcgcgtg
     2641 tctcgctcac gcggtagagc accaggtcgt tgcgtttgcg gtaccgcagc agccgcacct
     2701 tatcatcctc gccggccctc agaaaataat caatcgcttc ctgcaagggg acagtctcgc
     2761 gtataaaagc gacgtcctgc cgcaccagtt cgcgcatagc ggcttccagc cggttcaggt
     2821 ccgccctgga gaaatccggc acgttttcca cctggcaata gtagccgccg gaggaaacgg
     2881 aatggtcaat cgtcaggacg ccgcctggga agcactgctt gaaggctact tccatcagga
     2941 aggtgagcga gcgccgataa atgcgcgcgc cgtccgcgtc cctcagcgtc agcggagtga
     3001 ccagtgcgtc gcgtcggatc gggaaggtca gctcgcgcaa gttgccgtcc acaattgcac
     3061 ccacaatcat ggcttcctgc gatttttcca gcacgcgcaa gaactccata agcgccgcgc
     3121 cgcgcctgcc agtgataatt cgcccgtcgg gcaggctgag ctctaccgtc tcccgcgctt
     3181 tatccgaaag ataaaattca ttgtgttcca tcagctgctc ccgcaaaggt cagcctgcct
     3241 tcaggcagcg ccgccttgtt cttcttctgt tatggttttt ttgcgcgcaa gccgctgcca
     3301 gtctgggagg gcgcgcgtat gcatttatga ttatatccgc cggcgcagcc ccgcatcttt
     3361 ctgcttatcc ctgaagagaa agcgcgaaga tgccgggcgg gtcaaatcaa catattctgt
     3421 gccgccgcgc aaagcgcaca cagctgcctg cgaatgcgcg gccgagctca gggttggctc
     3481 aggcgcacga ttaaggtctg tcctccgttc aataccggcg ccacaaggaa gtcttttaag
     3541 gagccgtccg cgccaaagct gatttcatcg aacagcggct ctccccacag ggtttcggcc
     3601 tggtagcttc ccgaaagcgg gctgccttcc agggcgatgg tgcagttttc cgagctggga
     3661 atgcctgtgc tggccagtgt cagcaaaata tcatcttccg actggcgcag caccgcgtaa
     3721 acccgcctac agctggaggt cagcggcagg taaaccccgc cgcgcaaggc ctggtgctga
     3781 ttgcgcagtt gaatcagacc cttgtaccag ttgagcaggg agtctggatt ggcagcttgc
     3841 gtctcaacgt tggtttcctc ccaacctttg ttcacgggtt cccagggtat tccgctggtg
     3901 aaagcgctgt caggctctgt gctccactgc atgggcgtgc gcaggttctc gtccggcttg
     3961 ctgccggtca tcccgatttc ctcgccgtaa tacacaaacg gcgtgccggg accggtcagg
     4021 tagataaagg cggccacgcg ggctttgctt tccttgccgc cgaaatacga catcgggcgc
     4081 tgctggtcgt gattggagag gaaggtgctg aactgcaggt cgggaaagta cgcgagcacg
     4141 tccaggtagg atttgagcgc caaagaagca tcgccagaat acgccgcgcc tttgatatct
     4201 tccgccaggt cgaacataaa caggctgtcc aggcccttgg ggtggccgta tttggcagta
     4261 atctgcaaat ccgtccaaac ctccccgacg gtgtacgcct gggggttgag aggcttggta
     4321 aacgcgcgcc agtcctggaa ccactggatc gtttccttgg tgtcttgcag cgccgcgcct
     4381 tcctcgaaca ggtagcgcgc cgcgtccacg cggaagccgt ctacgcccag gtccaaccag
     4441 aacgcgctgg cgctctggat ttcttcggtc acggccgggt tgtggtagtt caggtcgggc
     4501 atcccgtccc agaagaccgc gtagtagaac ttgccgttcg aggcgcggta ccaggcgcgc
     4561 tgcccccacg gccccagcga gctagggtcc gaatccgacc agacgtagta gtctttatat
     4621 ttcgggtcgc cagcaagcgc ggccttgaac caggggtgtt gggtggaggt atggttcact
     4681 acgaaatcca taattacgcg gattccgcgc gcgtggcagg cttccagcag ctgccggaag
     4741 tcatccagcg tgccatagtc cgggttgacc gctttgtagt cggtcacgtc atagccgtgg
     4801 taggaaggcg aggggttgat gggcatcagc cacagcgcgc caacccccag gtcagtgttg
     4861 gtggcggggt cgccgtcgtt caggtagtcc aactgctgga tgatgccctg aaaatcgcca
     4921 atgccgtcgc cgtcgctgtc cttgaaggag cgcacaaaga tttcgtaaaa gaccacgtcg
     4981 ttccaccagc ctgaaagctc ttcgggcgtg ctttccgcgg tcggggtcag cgctggcgcg
     5041 gcggaggtcg tgctttccgc gggcggggtc agcgctggcg cggcggtcgg cgcgggctgg
     5101 caggcagcca gcaggaagag cagtgccgcc aatactccgc ttacgaactt atgctgtttc
     5161 atgcgcgcct tccttattta tcggatgaga gctatatttt acttgaaatg gccgggccct
     5221 ggacgagcct ggaaagaacc attttttaag cctgtttgct taaagaattt cgggttcttt
     5281 ctgcgcgccc tgctgaaaag cgcgctcggc taaagcgcga aaggattacc cgttactgct
     5341 ttgcagcggc gcggcgcatt caggtccctc ctggttgggg ctgttcactg cgcggctcac
     5401 ctcccatgcg cgcagccagt ccgccggcgc cggccgcagc atggaaacaa gcgcggtctc
     5461 gctggtctcc agcagccagt cccacatatt ctccttgcgc aggaggacgg gcatgcgttc
     5521 atgcaccgga gcgaccgttt cattcgcggc ggtggtgatg atcgtgcagg tggagagcgc
     5581 ctcgcccggt tggtctttgg gctgccaggt ttcccacagg ccggcgaagg caaagggttc
     5641 gcccgagcgc atggaaaagt agaacggccg cgagcctttg gcgccagtct tctgccattc
     5701 gtaaaagccg tccgccagga taaggcagcg gcgcttgcgg aaagcgtact tgaaagaggg
     5761 tttctcgaag accgactcgg cgcgcgcgtt gatcaggctg ctgccgatgg cggggtcttt
     5821 ggcccagaaa ggcaccaggc cccactgcag ccagtccacc tgccggcttt ccggatcgcg
     5881 cagcgcggcg acgggctgcg agggggcgat gttgtagcgc cgcttccagt cgtcgggcat
     5941 ttgcccgagc gccagggcgg tctggaggtc gtcggaatca atggccatgc taaagcgtcc
     6001 gcacatgccg ggtcctttcg taatgccggg ttatttcttc cattatacgc ccgcatgaat
     6061 ggtgtcagtt cgtaggccgg cttgcgcttg taaagccgct gcaagatttg tcactgcgag
     6121 cccccgctgc cgacccaaaa catgctgagc atcacaatag ggggcgcggc agtctgcatt
     6181 tccgctgtat aaccctcgaa cagatagagt ttcgggcaga ttgccgcagt cgctggcgct
     6241 ccttcgcaat gacgaatcat tctagctctc accggtccag caaagcaggc agatttaggg
     6301 cgaagcaacc tgtagatgat gctcacgctt accctaaacc tcattctgga tgcttcgccc
     6361 ctacacccac gcggcgtcag gtcagaatcc ctttccggac gaggaaaaat cccttgtttt
     6421 cgccaaaact cttacaccaa ttcccgcatt cgtgctatca ttgacacagt gcctatgtct
     6481 gggtttagac aggctttcaa ctcatctaac aattgaataa ggagtggata tgcgtaattg
     6541 gttcagcaga ttgattgcag tcatcatcat cctcggagcg ctctctccgg ccgccctcac
     6601 ttcggccgaa gcccagacgc ccccaccccc accggaacct gagcgccgcg cgcgctgcct
     6661 gccgggccag ccctgtgagg acgacaacgg cgagcgcttc atgctcccca ccgaagaaac
     6721 gctggcagag cctgccgcca tcggcgacac cgacgattac ggctacacca tcaccagcac
     6781 gacctattcc tggattgacg ccactggcgg cacaaacacg gggaggtgtc ggcagcgcag
     6841 atcgaccgcg tggcagccgt cgatgctgcc acgcaaccgc tacgctaca
//
LOCUS       DAOQAD010000094         6808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_13628_length_6808_cov_5.462313, whole genome shotgun sequence.
ACCESSION   DAOQAD010000094 DAOQAD010000000
VERSION     DAOQAD010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13628_length_6808_cov_5.462313"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(448..1494)
                     /gene="queG"
                     /locus_tag="PKL60_07210"
     CDS             complement(448..1494)
                     /gene="queG"
                     /locus_tag="PKL60_07210"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNW95958.1"
                     /translation="MPLEAEIKAEALKLGFVVCGIARLAQPETYPAFQGWIEAGCHAG
                     MVYLARPESLNARADPASLLPESRSVICVGLPYPAPMPPAEDAPNGRGLIAAYALQPD
                     YHKVVRDKLELLGARLSELAQRAVKQRACVDSAPMLEKDFARQAGLGWVGRNSLLSAP
                     GYGSYLYLGELLTDLELTPDSALPGDPCRNCNLCVDACPTHALRPNRSVDARRCLSYL
                     TIEHRGAIPAELRPTLGRRVFGCDTCQSVCPLNQGGLGAASAQALISASPDLLEEFSL
                     DAAAWKARYKGIPVLRAKYAGYRRNLAVAMANDQTPGAEQALRDALEEELPPDLRETF
                     LWALEKLNRAEAGV"
     gene            complement(1517..2134)
                     /locus_tag="PKL60_07215"
     CDS             complement(1517..2134)
                     /locus_tag="PKL60_07215"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNW95959.1"
                     /translation="MNKKAISSVFFSAVMISMLLLSACSKSTPTPSPDQIMTSIAQTV
                     EVDFTRTAIARPTDTPTPLPTPTYTPVPPTATFPPTNTPAAGATATAVPGGRDAGVWV
                     SSNPADNSNITAGLPFDVVLTLMNTGTTTWTSAYAIKFISGDRLGASETISMPYEVPP
                     SKTVTLTMKFTAPSTAGKVRGDWAIVNAAGTSFSSFYFEYNIVTP"
     gene            complement(3047..4285)
                     /locus_tag="PKL60_07220"
     CDS             complement(3047..4285)
                     /locus_tag="PKL60_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95960.1"
                     /translation="MNKTWSVFRHEFLTTISRRSFLLTLILVPLIPALVLWILGSLTE
                     SQSQSLTQVFTGSQTNSALPYGAVDHSGLVMEAPDWVTGGSIVLIPSAEEARQQLQEG
                     KLKGYFEISQDYLQSGKVTLVREDVNPLTGFEDTAAMENLLTYNLLGRDQDMYLKYTN
                     PVSFVLEEVNPAIAETRDTEDPLNFFVPYGVTLFFYMIVFITSSMLLNTVGKDKDNRV
                     MEVLLSSVKPTNLFAGKLAGLGLASLLQLALWFGAALVLLRVTGQTFSLPIAINLPAS
                     LYAYGILFFIVGFIFYGSLMAGIGAMAPNLREGSQSTFFVALPLILTLVAINSLIQKP
                     HGGLATLLSLLPPTAPVAMMTRLTIGGVPGWQIGLALGLLALTDFFLVKGVSNLFRSQ
                     NLLTGSKFSFKLFLRELVRR"
     gene            complement(4282..5490)
                     /locus_tag="PKL60_07225"
     CDS             complement(4282..5490)
                     /locus_tag="PKL60_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95961.1"
                     /translation="MSKTFEVAKYEYTHHVARPRFWIALLSVPVGIVLVTLFAVAVSL
                     SSMSKLPVGYVDEAKLITIPFTAPEKPSLFEPVIPMIPFSSEEEARASAERGEIQAYF
                     VIPEGYESTFQIKYSFNEPLTSTIRGQIRSLLSQNLLAGMDIPNQARLEAGSLYTLVS
                     LDGTRSTGENEISKILVPLVVGLLFVIVVISSGGYLLQAVVEEKENRTIEIMVTSVSP
                     NELMAGKIIGNLSVGLTQILFWVLLAAVFVLALGERIPFLRDLQISWPNLLLSLLLLL
                     PCFVFTAGLMAILGATVTDSQEAAQVTGLVTFPILIPYYFFSVLMMHPNGAFARALSY
                     FPLSAPVAMTLRMAFTQVPIWELALVSAMLVLFSVLVIWLAGKAFRMGMLQYSRRIKL
                     SQLLKKAGAQ"
     gene            complement(5474..6391)
                     /locus_tag="PKL60_07230"
     CDS             complement(5474..6391)
                     /locus_tag="PKL60_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNW95962.1"
                     /translation="MQSIHVEHISKRFGDTQAVKDISFEVAPGEIFGLLGPNGSGKTT
                     SIRIILDIYQPDTGSVSILGGPMNEAKKNQIGYLPEERGLYQDILVDKCLEYLATLKG
                     MSPQQIKQKLPAYLEEFDLSAHKKKKVKELSKGMQQKAQLIATLIHDPQVIIIDEPFS
                     SLDPVNTQLVKDILKQQRDAGKTIIMCTHQMNQVEQLCDKIVLVDKGEVLLYGGLQQI
                     YESFATDDVRINTSMELPPAIPGVRKIERLNTMTKLEPQPGVKPQDILNTLVSQGIQL
                     NAFEIAVPSLDEIFIKVVKKGGADDEQDL"
BASE COUNT         1450 a         1850 c         2056 g         1452 t
ORIGIN      
        1 gcccaaagtg gaagaacgtg cctggtatgg gcctcgtttt gacagcgcaa attcgtaaac
       61 tcctataaaa cggacgcggg gtggaaaatt tataagggcc gtccgacttt tgggaaagcc
      121 cccgtctcta cgggtatctt cttccaacag gaaggtgaat taactgtcgg acgtcgatat
      181 cttcgaaaac ttctcctggg gatacgaggc tgatgagggg cttttcttcc tgttcgtttg
      241 cagtctgcct tcttggaact gccaattcgg gcgaagcatc ccaggtcgat tacctcgcag
      301 gatttgtcag gctgtatacc gagggcttcg accaactcca gatgaagggc acatcagtag
      361 gattaatgaa gtgacgcaaa gcaggaaacg cgcgtcctgc catcccttcg agcccccatg
      421 ggatggcagc atatcagccg ttccggccta cacccctgct tcagcccggt tcagcttctc
      481 caacgcccag aggaaggtct cgcgcaaatc cggcggtaac tcctcctcca gcgcgtctcg
      541 caaggcttgt tcagcccccg gggtttggtc gttcgccatc gcgaccgcca gattccgccg
      601 gtaacccgcg tactttgcgc gcagcactgg aatgcccttg tagcgcgctt tccatgccgc
      661 tgcgtctaag gaaaactctt ctaacagatc cggagatgct gaaattagcg cttgggccga
      721 agcggcgcct aagcccccct gattgagcgg gcagacgctc tggcaggtat cgcacccgaa
      781 gactcgccgc ccgagcgtgg ggcgcagctc cgctggtatg gctccgcggt gttcgatagt
      841 caggtaactc agacagcggc gggcatccac ggagcggttg gggcgcagcg catgggtggg
      901 gcaggcgtcc acgcataaat tacagttccg gcatgggtcg cccgggagcg ctgaatcggg
      961 ggtcagttcc aggtcggtca gcagctcccc aagatacaag taagagccgt aaccgggcgc
     1021 actcaagagc gaattgcgcc cgacccagcc caggccagcc tgcctggcaa agtccttttc
     1081 aagcatcggc gcgctgtcca cgcaagcccg ctgcttcaca gcgcgctgag ccagctccga
     1141 aaggcgcgcg cccagtagtt ccagtttatc cctgaccact ttgtgatagt ctggctgcag
     1201 cgcgtaagcc gcgatgagac ctcttccgtt cggggcgtcc tcagcaggcg gcataggcgc
     1261 ggggtagggc aaacccacgc aaatgacgga gcggctttcg ggcaggaggc tggcggggtc
     1321 ggcgcgcgcg ttcaggcttt cgggtctggc gaggtaaacc atcccggcgt ggcagcccgc
     1381 ctcaatccag ccctgaaacg cagggtaggt ttccggttgt gccaggcggg cgatgccgca
     1441 aacgacaaag ccgagcttga gggcctcggc tttgatttct gcttccagtg gcatggcgcg
     1501 ctccactaag ggcagcttaa ggcgtcacaa tgttgtattc aaaatagaag ctggagaagg
     1561 acgtgcctgc cgcgttgacg atggcccaat cgccgcgcac ctttccggca gttgacggcg
     1621 cggtgaactt catggtcaag gtgacggttt tgctgggcgg cacttcgtag ggcatgctta
     1681 tggtttcgct ggcgccaagc cggtcgccgc tgatgaactt aatcgcatag gcgctggtcc
     1741 aggttgtggt gccggtgttc atcagagtca ggaccacatc aaagggcagc ccggccgtaa
     1801 tattggaatt gtccgccgga ttgctggaga cccacacgcc tgcgtctcgc ccgccgggaa
     1861 cagcggtggc ggtggcgccg gcagccggcg tgttggtcgg cgggaatgtg gccgtgggcg
     1921 gaacgggggt gtaagtcggg gttggcagcg gtgtgggggt atccgtcggg cgggcgatgg
     1981 ctgtgcgcgt aaaatccacc tctacggtct gggcaatgct ggtcataatc tggtcggggc
     2041 tgggggtagg ggtgctcttg gagcaagccg aaagcagcag cattgaaatc atcacagcgg
     2101 agaaaaatac gctcgaaatt gcttttttat tcatgtagaa ctccatccag cgcgaaattc
     2161 cctgtaacag gcgcatgatt ttctattgta gagcttcagc caaatttgtc aagtcagacc
     2221 cgatatcggc gaggtacttt tgagcgcgga attgcgctcg attttggcag ttcttttcat
     2281 cagggaagcc ttcagactca cgagaaagga gctcgcgagg aaacatgggt ttagcaaatg
     2341 cgcacggaca tgaacagatg caggatgtca tccgcctccg agccgcgagc gtccgctgag
     2401 ttcaagaatt ggagccatgg gaaaacccct tttcaaagga agattgagca cccaacctac
     2461 cacaggggat agaaaagcgg gctggcaatt agccagcgcg ccgggtactg tgctgcgcgc
     2521 cgaaacgcgc cgttgctgct ctcgctccag gcggcgaaga agcgcggagc ccggaagccg
     2581 gtccgcgcaa gcaaggaaga gtcagcgctg cgggccgcca gcctacagcc tgccagcctc
     2641 aaccatattc aacgggtatg gtccggttct attgccacgg cttttctgcc ttatggggag
     2701 cgcaggttag cgtctctgtc tatggtgtaa attcattgtg tgggagcgca gggcctgagc
     2761 tgcgtctttg gattattttc tcttttttgc ttccacaaag tagggacata cgcgcttttt
     2821 tcccccgcgc ttgaggatta acccgccagc tctgattcag gatgcgcgtt tgagcggcgc
     2881 aggtccgggg gaaaggcagc ttattcgagg ccaggcggat ggttgcaatg tccaggtaaa
     2941 aaagcgtgca gcggtgcagg ttcaatcacc cctgggaaac gcggttctgc cgggctgacg
     3001 aaaaaccccg cccgaaaggg cggggtttga gcacaaacag gcgggcttat ctgcgcacca
     3061 gttcgcgcaa aaagagcttg aaggagaatt tactgccggt aagcaggttt tgcgagcgga
     3121 ataggttcga tacgccttta accaggaaga agtcggtcag cgccaaaagc cccagcgcca
     3181 gccctatttg ccagcctggc acgccgccga ttgtcaggcg ggtcatcatc gcgacaggcg
     3241 cggtgggagg aagcaggctg agcagggttg ccaacccgcc gtggggtttt tggatcaggc
     3301 tgttgattgc taccagcgtc agaatgagcg gcagcgccac gaaaaaggtg gactggctgc
     3361 cctcgcgcag gttgggagcc atcgcgccga tgcctgccat caagctgccg tagaagatga
     3421 acccgacgat aaagaacagg attccgtagg cgtagagcga ggctggaagg ttaatcgcta
     3481 tcggcaggga gaaagtctgc ccggttaccc gcagcagcac cagcgcggcg ccaaaccaga
     3541 gcgccaactg cagcaggctg gccagcccca gacccgccag cttgccggca aacaggttgg
     3601 tgggctttac cgaggaaagc agcacttcca ttacccggtt gtctttatcc ttcccaactg
     3661 tgttcaacag catgctggag gtgatgaaca cgatcatata gaagaacagc gtgacgccgt
     3721 aaggcacaaa aaagttgagc ggatcttcgg tgtcgcgcgt ttcagcaatg gcggggttga
     3781 cctcttccag cacgaacgag accggattcg tgtacttaag atacatatcc tggtccctgc
     3841 cgagtaagtt gtacgtaagc aggttttcca tcgcagcggt gtcctcgaag ccggtgagcg
     3901 ggttgacgtc ttcgcggacc agggtgacct tgccgctttg caggtagtcc tgtgaaattt
     3961 cgaaatagcc tttcagcttg ccttcctgca gctgctgccg ggcttcttcc gcggagggga
     4021 tgagcacaat gctgccgccg gtaacccaat ccggggcttc catcaccagt ccgctgtggt
     4081 cgaccgcgcc gtaaggcagg gcggagttgg tttgcgagcc ggtaaacacc tgcgtgaggc
     4141 tttgggattg cgactcggtc aagctgccca ggatccacag cacaagcgcc ggaatgagcg
     4201 ggacaaggat aagcgtgagg agaaatgaac ggcggctgat ggtcgtcagg aactcatgtc
     4261 tgaaaacgct ccaggttttg ttcactgcgc gcctgctttc tttaatagct ggctgagctt
     4321 gatgcgcctg gagtactgca gcatacccat gcggaaagct ttacccgcca gccagatgac
     4381 aagcacggaa aacagcacca gcatcgcgct gaccagcgcc agttcccaga tgggcacttg
     4441 cgtgaacgcc atacgcaggg tcatcgccac gggcgcgctg agcgggaagt aacttagcgc
     4501 ccgcgcgaaa gcgccgttcg ggtgcatcat cagcaccgag aagaagtaat acggaatgag
     4561 gatggggaag gtaaccagac cggtcacctg ggcagcttcc tgcgaatccg tcaccgtcgc
     4621 gccaaggatt gccatcagcc cggcggtgaa gacaaaacag ggcagcagca aaagcaagct
     4681 gagcagcaaa ttcggccacg aaatctgcaa atcccgcagg aaggggatgc gctcgcccag
     4741 ggcgagcaca aagactgccg cgagcagcac ccagaacagg atttgcgtca gaccgacgct
     4801 gaggtttccg ataatcttgc ctgccatcaa ttcattcggg gagactgatg tcaccataat
     4861 ctcgatggtg cggttttctt tttcctctac caccgcctgc agcagataac cgccgctcga
     4921 gattacgaca atgacaaaaa gcaggcccac caccagcggt acgaggattt tggagatttc
     4981 gttctcgcct gtgcttcggg ttccatccag ggaaaccaag gtgtacaggc tgccggcttc
     5041 caggcgggct tggttgggaa tgtccattcc ggctaaaagg ttctggctca gcaggctgcg
     5101 gatttggccg cggattgtgc tggtcagcgg ttcgttgaag gaatacttga tctgaaaagt
     5161 cgattcatag ccttccggga taacaaagta ggcttggatt tcgccgcgtt ccgctgaggc
     5221 gcgcgcttcc tcctccgagc tgaaggggat catcgggatg accggctcga acaaagacgg
     5281 cttttcaggg gcggtgaagg gaattgtgat taacttcgct tcgtccacgt agcccaccgg
     5341 cagcttgctc atcgacgaga gcgaaaccgc aactgcgaaa agcgtgacaa ggactattcc
     5401 caccggcacg ctgagcagcg cgatccagaa acgcggccgg gcgacatggt gggtatattc
     5461 gtatttggct acctcaaagg tcttgctcat cgtcagcccc tccttttttt accaccttga
     5521 tgaagatttc atcgagagag ggcaccgcga tctcgaaagc attaagctga atgccttgcg
     5581 aaaccaacgt gttcagaata tcctgcggtt ttacgccagg ctggggttca agttttgtca
     5641 tggtattcag ccgttcgatc ttccgcaccc cggggattgc cggggggagt tccatgctgg
     5701 tgttgatgcg cacatcgtcc gtggcgaagc tttcatagat ctgctgcaga ccgccgtaaa
     5761 gcagcacttc gcccttgtcg accagcacga tcttatcgca gagctgctcc acctggttca
     5821 tctggtgggt gcacatgatg atcgttttgc cggcgtcgcg ctgttgtttg aggatgtctt
     5881 tgaccaattg ggtgttgacc gggtcgagcg agctaaaggg ctcatcgatg ataatcactt
     5941 gcgggtcgtg gatcagcgtg gcaatcagtt gggctttctg ctgcatgccc ttgctcagtt
     6001 ccttgacttt tttctttttg tgagcgctca ggtcgaactc ctccaggtag gcaggcagct
     6061 tttgtttaat ttgctgtggg ctcatgcctt tcagggtggc aaggtattcc agacatttat
     6121 ctaccaggat gtcctgatac aagccccgct cttcgggcag gtaaccaatc tggttctttt
     6181 tggcttcgtt catcgggccg ccaaggatgg ataccgagcc ggtgtctggt tggtaaatat
     6241 ccaggatgat gcggatgctg gtggttttgc cggagccgtt gggacccagg agaccgaata
     6301 tctcccccgg cgctacttcg aaggatatat ctttgacagc ttgcgtatcc ccgaagcgtt
     6361 tgctgatgtg ctcaacatga atagactgca tggttcatct ccaatccgat tttcttatta
     6421 gcggagtaat catacccgat gaaaagcttg tgaaacagag aaacatgatg ttaagcataa
     6481 atccggagga ttttgacaac ccaagaaaat atggggttaa tgggggtgtc attctgaacg
     6541 atgggaagac tctcttgtgg gaaaaacaga tccttggctg gcgctcagga gaataagccg
     6601 tttaagctgg gtgcagcggc gcggggccga acccaatgcc ggctccacaa acccgttggc
     6661 caaggcttgt cgggttgcgc tggcgctcca cccgacctac gatcacgttt tgtcgggttg
     6721 cgctggcgct ccacccgacc tacgagcacg gtctgtcggg ttgcgctggc gcttcacccg
     6781 acctacggct ccacccgacc tacgagca
//
LOCUS       DAOQAD010000095         6786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_13685_length_6786_cov_4.806269, whole genome shotgun sequence.
ACCESSION   DAOQAD010000095 DAOQAD010000000
VERSION     DAOQAD010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13685_length_6786_cov_4.806269"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PKL60_07235"
     CDS             complement(<1..930)
                     /locus_tag="PKL60_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNW95963.1"
                     /translation="MATKKWVYLFDEVEQAEAYAGSWDGVRGLLGGKGSNLAEMVRIG
                     VPVPPGFTITTEACNAFQETNQFPEGMWQQTLDALKQVEKKAGKVFGDPKNPLLVSCR
                     SGAKFSMPGMMDTVLNIGMNDETARGMIALTGNERFVADSYRRLVQMFGSVVLGIADE
                     AFDEVLDSYKARKGVKLDTELGAADWQYLTEKFKEVVKREMGFDFPQDPLVQMEYAIK
                     AVFGSWNSKRAIDYRRREHIPDTLGTAVNIQTMVFGNMGDDSGTGVAFTRDPQTGENK
                     MFGEYLLNAQGEDVVAGIRNTSKIEGLKAEIPDA"
     gene            1233..1601
                     /locus_tag="PKL60_07240"
     CDS             1233..1601
                     /locus_tag="PKL60_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95964.1"
                     /translation="MLEGRTRRCASQEANAVFASIRLNLADEQTENMLIKLLSWQWPD
                     GAGTATSARKPRIPLSGRPGRPCALLTWKAYTGSSPALKEAIAQAVQFFLQKRLFLRE
                     SSGEPMQPGFTRLHQPPYWK"
     gene            complement(1862..2401)
                     /locus_tag="PKL60_07245"
     CDS             complement(1862..2401)
                     /locus_tag="PKL60_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNW95965.1"
                     /translation="MDALESIYKRRSIRKFTDVPVEDDLIEQLLRAAMAAPSAMNMKP
                     WRFVVVREPERLERLRKVLPFGKMNASAAISVCGDLRSMRRVVTENFWQQDCSAATEN
                     LLIAASALGLGAVWCGVTPLARLQKAVASVLELPEGVLPLNVVLIGYPAEEKPARTQY
                     DPDLVFLDSYGTPWKKESN"
     gene            complement(2410..3171)
                     /locus_tag="PKL60_07250"
     CDS             complement(2410..3171)
                     /locus_tag="PKL60_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNW95966.1"
                     /translation="MKIQLEHIAMEVESLGTGKSIVLLHGLGLNHSIWQNIAGLYKEQ
                     AQFILPDLRGHGGSELGAADGTIEQYADDILELLDKLELEKVVLGGHSMGGYVALAFA
                     EKYPQRVAGLVMIATNAGPDSDARRAQRLAEVENIRAVGTAEFAEGLSLRLSDRSFVQ
                     NVSREIIVNTDPEGLCNAIIAIARRPDRLHVLTGLKAPILIAAGAEDQIVPQAAARLM
                     ARSNPNAKFVILPEVGHMPMLSAPRTLGALLIVTG"
     gene            complement(3168..4676)
                     /locus_tag="PKL60_07255"
     CDS             complement(3168..4676)
                     /locus_tag="PKL60_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNW95967.1"
                     /translation="MSLFPDERTRRIFDAVLNSLDAPMPVYLVGGAVRDRLLGLPVND
                     LDFVVPSHSLQLAGAVRRKLGAAGFTLDDKRQTARLILGQGKTSELILDFVSFTGGSL
                     YEDLSNRDFTINTLSVALDQPDVLIDLLGGERDLSEKCLRAASASSMELDPLRVLRGV
                     RLALAYQLSIEPETLALMQNSVTSLERVSGERVRDELFKILEHKDPETAFRQMDALGL
                     TEQVFPELRALKQVPAYPPHVHDLWTHTLQVMRYLRDVWNFLQTGEFPETNPFLGETC
                     AALAPYTQSLREILSKPVQANRPRWSLLLLAALYHDAGKPAAQTTDATGRVRFIGHDR
                     LSAELVRIRAQALMLGRDEVYYLQQLAANHMRLHFFSREGTQLSDRACYRYFRDMNEF
                     GVDAVLLSLADMLAAYESTLASANWQRELQAGITLLDAWFSRRQQLVDPKLLLDGDDL
                     QREFHLAPGALIGKLLAQLREAQAGGEVCTRAEALVFVEKYLRTKDAKERFQ"
     gene            complement(4660..5217)
                     /gene="hpt"
                     /locus_tag="PKL60_07260"
     CDS             complement(4660..5217)
                     /gene="hpt"
                     /locus_tag="PKL60_07260"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNW95968.1"
                     /translation="MQDYQNFLSEVLISEEQLCARIKELGAEISRDYAGKELLVVCIL
                     RGGVMFMTDLVRQISIPLAFEFMAVSSYGSGARRSSGDVRITLDLNTNIAGRHVLIVE
                     DIIDSGRTLASVIDLLKTRDPASLYVCTLLDKVSRREVEVPVRYRGFAIEDKFVFGYG
                     LDMDDYYRNLPFIGVVDLKKYESLS"
     gene            complement(5228..5800)
                     /locus_tag="PKL60_07265"
     CDS             complement(5228..5800)
                     /locus_tag="PKL60_07265"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNW95969.1"
                     /translation="MAHQYGSVEVITGSMFCGKTEELIRRLRRATIARQKVQVFKPII
                     DDRYAFAKVTSHSGTDFDALPIGSSGEILAHLEPGTTVVGIDEAQFFDNGIVDAVNQL
                     ADRGIRVIVTGLDTDFRGEPFGCMPALIAKAETVDKLNAICMVCGEPASRTQRLVNGK
                     PAHYNDPIVIVGASEMYEARCRRCHQVPRD"
     gene            complement(5873..6352)
                     /gene="rpmE"
                     /locus_tag="PKL60_07270"
     CDS             complement(5873..6352)
                     /gene="rpmE"
                     /locus_tag="PKL60_07270"
                     /inference="COORDINATES: protein motif:HMM:NF000612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNW95970.1"
                     /translation="MKTKIQPKYYPQAKVTCASCGATWTTGSTVPEIRTEVCSNCHPF
                     YTGQQARLLDREGQVDRFYKRLQVRQDYVSDKKAMAEAKTSLNRPLTDLNLSTKAIAA
                     LAAAELTTVGHVLTRLEGGEKALLEVEGFGRKSLIDLKKQLRQLGYELPAAASEIEV"
     gene            complement(6374..6634)
                     /gene="rpmA"
                     /locus_tag="PKL60_07275"
     CDS             complement(6374..6634)
                     /gene="rpmA"
                     /locus_tag="PKL60_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNW95971.1"
                     /translation="MAHKKGGGSSRNGRDSQGQRLGIKKYGGEHVISGNIIVRQHGTK
                     VHPGWNVGCGRDYTLFATAEGTVRYETLPNGRKKVHVVVAAE"
BASE COUNT         1444 a         1988 c         1850 g         1504 t
ORIGIN      
        1 agcatccggg atttcggctt tcaaaccttc aattttggag gtgttgcgga tgccggcgac
       61 cacatcttcg ccctgggcgt tgagaaggta ctcgccgaac atcttgtttt cacctgtctg
      121 cgggtcgcgc gtgaacgcta cgccagtgcc ggagtcgtcg cccatattgc cgaacaccat
      181 ggtctggata tttacagccg tgccgagtgt gtcggggata tgctcgcggc ggcggtaatc
      241 aatcgcgcgt ttggagttcc aggaaccgaa gacggctttg atggcatact ccatctgcac
      301 cagcggatcc tgcgggaagt cgaagcccat ttcgcgcttg acaacctctt tgaatttttc
      361 agtcaggtac tgccagtctg ccgcgcccag ttcggtatcc agctttacac ccttccgggc
      421 tttgtagctg tccagaactt catcaaaggc ttcgtcggcg atgccgagta caacgctgcc
      481 gaacatctga accaggcggc ggtacgagtc cgccacaaag cgctcattgc cggtcagcgc
      541 gatcatgccc cgcgcagttt cgtcattcat gccgatgttg agaacggtgt ccatcatacc
      601 gggcatggag aatttggcgc cggaacggca ggaaacaagc agtgggtttt tggggtcgcc
      661 aaaaaccttg ccggctttct tttccacttg cttcagggcg tccagggttt gttgccacat
      721 tccctcgggg aactgattcg tttcctgaaa cgcattacag gcttcggttg taatcgtgaa
      781 accaggggga accggcacgc caatacgtac catctcagcc aggttggaac ccttgccgcc
      841 caaaagtccg cgcacgccgt cccaactgcc cgcgtatgct tccgcctgct cgacctcgtc
      901 aaacaggtat acccatttct ttgttgccat acagcctcct aagctgaatc gtaaaaaaaa
      961 tacgtaatct gattgtgata tctctaccgt gaaataatcc aacgaaaaat tttaccatac
     1021 ttccccccgt tctggctttc catatagagt cttggaagag caaaggaata atgtccaaaa
     1081 atagaaatac cctcattaaa ttaggagctg tgtaatcctt attcgattcc tccaggttct
     1141 tttacaaaga aatatgattc cgcggacaca caagggaaca gcagtctttc ccctcttact
     1201 cattgctcca gcggctgaac tgatgcttcc gcatgctgga gggacgtacc cggcgctgcg
     1261 ccagccagga agccaacgca gtgtttgcct ccatccggct caacctggca gatgaacaga
     1321 ctgagaacat gctaataaag cttttgagct ggcagtggcc ggacggggct ggaactgcga
     1381 caagcgcccg gaagcctcgc attcctctat ccgggagacc tggacgccct tgcgcgctgc
     1441 tgacctggaa agcatacacc ggcagcagtc cggccttgaa agaagccatc gcacaggccg
     1501 tgcagttctt tttacagaaa cggctgttct tgcgggaaag cagcggggag ccgatgcagc
     1561 ccgggttcac gcgtctgcac cagcctccct attggaaata ggacattctg gtggggctgg
     1621 agctgatgcg ccaggctggg aaactggagg acccgcgctg cgagccggcc ttaccgttgg
     1681 tagaggcaaa aatgcgccct gacggcggct tttacgcgga ggagaagcag tttcaggcgt
     1741 ccaatcccgc tgcctggcgt tacccctcct tagactgggg cggaccgtcc gctaaaaaga
     1801 tgaacatttg gctgactgtg cgggacttgg ctgttttgaa ggccgccggg aagattcgct
     1861 tttagttact ctcttttttc cagggcgtgc cgtacgaatc caggaaaacc aaatccgggt
     1921 cgtactgcgt tcgggcgggc ttttcttccg ccggatagcc aatcagaacc acattgagag
     1981 gcagcacgcc ttcgggcagt tcgaggactg aagcaacggc tttttgcagc ctggcgagcg
     2041 gcgtgacccc acaccatacc gcccccagac caagcgcaga agccgcgata agcaggtttt
     2101 cggtggcagc gctgcagtct tgctgccaaa agttttcggt gacgacgcgc cgcatagagc
     2161 gcaggtcgcc gcaaacgctg atagcggcgc tggcgttcat cttcccaaag ggcagcactt
     2221 tgcgcaggcg ttccaggcgt tcaggttccc tgacgaccac aaaacgccac ggcttcatgt
     2281 tcatcgcgga aggcgcggcc atggcggcac gcaagagctg ctcaattaaa tcgtcttcca
     2341 ccggaacgtc cgtgaatttg cggatgctgc ggcgtttgta aatactctcc agcgcgtcca
     2401 tgcctaccct caaccggtga cgatgagtaa ggcgcccagt gtgcgtggag ctgacagcat
     2461 cggcatatgc cctacctccg gtaaaatcac gaatttggcg ttcgggttgg agcgcgccat
     2521 caggcgcgcc gctgcctgcg gaacaatctg gtcttcagcg cctgccgcaa tcaaaatggg
     2581 ggcttttaac ccggtcagta catgcaggcg gtctggtcgg cgggcaatcg cgatgattgc
     2641 attacaaagg ccttcagggt cggtgttgac aatgatttcc cgcgaaacgt tctgaacaaa
     2701 cgaacgatcc gacaacctca ggcttagccc ttcagcaaat tcagcggtgc cgactgctct
     2761 gatgttttcc acttccgcca ggcgctgcgc tctgcgggca tcgctgtccg gacccgcgtt
     2821 ggtggcaatc atcaccaggc cagccacccg ctgggggtac ttttcggcga aggccagggc
     2881 gacgtaaccg cccatggaat gcccgcccaa aacgaccttc tccagctcaa gcttgtctaa
     2941 caattccagg atatcatcgg catactgctc gattgtgccg tcagcagccc caagctcact
     3001 gccgccgtgc ccgcgtaaat cgggcaggat gaactgcgcc tgttccttgt acagcccggc
     3061 aatgttctgc cagatgctgt ggttcagccc aagcccgtgc agcagtacaa ttgatttgcc
     3121 cgttcccagg ctttcaactt ccatcgcgat atgctcaagt tgaattttca ctgaaacctc
     3181 tcctttgcat ctttggtgcg tagatatttt tccacaaaaa ccagggcttc agctctggtg
     3241 cagacttcac cgcccgcctg agcttccctg agctgcgcga gcagcttacc aatcaaagcg
     3301 ccaggcgcca ggtggaattc ccgctgcaaa tcgtcaccgt ctaacagcaa cttggggtcc
     3361 accagctgct gcctgcgcga gaaccaggca tccaagagag tgattcccgc ctggagttcg
     3421 cgctgccagt tcgcggacgc aagcgtgctt tcatatgccg ccagcatatc cgccaggctg
     3481 agcagtaccg cgtccacgcc aaactcgttc atgtcccgga aataacggta gcaagcccgg
     3541 tcgctcagct gcgtgccttc cctggagaag aaatgcaggc gcatgtggtt tgcagccagc
     3601 tgctgcaggt aatagacttc atcgcgcccc agcatcagtg cctgcgcgcg tatcctgacc
     3661 aattcagcgc tgaggcggtc gtgcccgata aagcgcaccc gacctgtggc gtccgtcgtc
     3721 tgcgccgcgg gtttgcccgc gtcatgatag agcgccgcca gcagcagcag actccagcgc
     3781 gggcggttgg cttgtacagg tttggacaga atttcgcgca ggctctgcgt gtaaggcgcc
     3841 agggctgcgc aggtttcccc caaaaatgga tttgtttcag ggaattcgcc ggtctgtaga
     3901 aaattccata catcccgcag gtagcgcatc acctgcaacg tgtgtgtcca caggtcgtgg
     3961 acgtgcggcg gataggcagg cacctgtttt agcgcacgca gctccgggaa tacttgttct
     4021 gtaagcccca aagcatccat ctgccggaaa gctgtttccg ggtccttatg ttccagaatc
     4081 ttgaacagct catcgcgtac gcgttcgccg gagacgcgct ccaggctggt gacagaattc
     4141 tgcatcaacg cgagtgtttc gggttcgatg ctgagctggt aggccagcgc caggcgtacg
     4201 ccccgcagca cacgcaaagg gtccagttcc attgagctgg cggaggctgc ccgcaggcat
     4261 ttctcgctca ggtcgcgttc ccctcccagc aagtcaatca ggacgtccgg ttgatccagt
     4321 gccacggaga gggtgttgat ggtgaaatcc cggttggaca ggtcctcata caggctgccg
     4381 ccggtgaagc tcacaaaatc caggatgagt tcggaggttt tcccctgacc gagaatcagg
     4441 cgcgcggtct ggcgtttatc atccagcgta aaacccgccg cgcccaattt gcgccgcaca
     4501 gcgccagcca attgcaggga atgggacgga accacaaaat caagatcgtt cacgggcagc
     4561 ccaagcagcc tgtcccgtac ggccccgccc accagatata caggcatagg cgcatccagg
     4621 ctgttcaaaa ccgcgtcgaa aatcctgcgg gtgcgttcgt caggaaagag actcatactt
     4681 cttaaggtca accactccga taaatggcag gtttcgatag taatcatcca tatcgaggcc
     4741 gtaaccgaac acgaatttgt cctcgatcgc gaacccgcgg tagcgcacag gcacctccac
     4801 ctcacggcgg gaaactttat ccagcagggt gcaaacatat aaggaggcgg gatcgcgggt
     4861 tttcagcagg tcaatcacgg atgccagcgt tctgccgctg tcgataatgt cttcaacaat
     4921 cagaacatga cgccccgcga tgttggtgtt caggtcgagt gtgatgcgca catcgccgga
     4981 agagcgccgc gcgccgctgc cgtaagaaga aaccgccata aactcgaatg ccaatgggat
     5041 gctgatctgg cgcaccaggt cggtcatgaa catcacccca ccgcgcaaga tgcaaaccac
     5101 gagcagttct ttgcccgcgt aatcgcgcga gatttccgcc cccaattcct ttatcctcgc
     5161 gcagagctgc tcctcagata tcaatacttc gctcaaaaaa ttctggtaat cctgcataca
     5221 cagcctctca atccctcggc acctgatgac agcgccggca gcgcgcttcg tacatttcgg
     5281 acgcgccgac aatcacaatc gggtcgttgt agtgcgctgg tttcccgttc accaggcgct
     5341 gcgtccgcga agccggctcg ccgcatacca tgcaaatcgc gttaagtttg tccaccgttt
     5401 ccgctttggc aatcaacgcc ggcatacagc cgaaaggttc cccccggaag tctgtgtcca
     5461 gacccgtgac aatcacgcgg ataccgcggt cagccagctg gttgaccgcg tccacgatac
     5521 cgttgtcaaa gaattgggcc tcatcaattc ccacgaccgt tgttccgggt tccagatgcg
     5581 cgaggatttc accggaggat ccaatcggca gcgcgtcaaa atccgtgccg gagtgagaag
     5641 tgactttcgc gaaggcgtag cgatcgtcga tgatcggttt gaagacctga accttctggc
     5701 gcgcgatggt tgcccgcctg agccgtctga taagttcttc ggttttccca cagaacatcg
     5761 agcctgtaat aacttcgact gaaccgtatt ggtgcgccat gcgtcctcca aaagaaaaag
     5821 gcaggtacaa aacctgcctt taagtttacc ggataaactc cgtctgtgcg agttaaacct
     5881 caatttcaga ggcagccgcg ggcagttcat agcccagctg gcgcagttgt ttcttgaggt
     5941 caatcagcga ttttctgccg aagccttcga cctcgagcag agccttttcc ccgccttcca
     6001 gccgggtgag aacatgcccc acggtggtca actcggcggc tgccagcgcg gcaatcgctt
     6061 tcgtggaaag attgaggtca gtcagagggc gattcaggga agttttggct tcagccatgg
     6121 cctttttgtc cgaaacgtaa tcctgccgga cctgcagacg cttgtagaag cggtccacct
     6181 gaccttcgcg gtccaaaagg cgtgcctgct gacccgtgta gaaggggtgg cagttggaac
     6241 agacttccgt acgaatttca gggaccgttg aaccggtcgt ccaggtagca ccacaggaag
     6301 cgcaggttac ctttgcctga ggatagtatt tgggttgaat tttggttttc attttttacc
     6361 tttgcagcgc tgtttattct gcggcaacaa cgacatggac ctttttgcgg ccgttaggaa
     6421 gggtttcata ccggactgta ccttccgcgg tggcaaaaag cgtgtagtcc cgcccacagc
     6481 ccacgttcca tccgggatgg accttggtgc catgctggcg gacaatgata ttgccgctga
     6541 tgacgtgttc gccgccatat ttcttgatgc cgaggcgttg tccctggctg tcacgaccgt
     6601 tgcgggatga cccgccgcct tttttatgtg ccatcttgtc ctactccatc gaaattgatt
     6661 ccaccagcag tctggtgtac ttttggcggt gaccggtgtg ccggcggtaa gcaatcttgg
     6721 gtttgtattt gaacaccagg attttctcac ccttcaactg ctccactaca gtggtcttca
     6781 ggctgg
//
LOCUS       DAOQAD010000096         6694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_13951_length_6694_cov_5.486971, whole genome shotgun sequence.
ACCESSION   DAOQAD010000096 DAOQAD010000000
VERSION     DAOQAD010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13951_length_6694_cov_5.486971"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PKL60_07280"
     CDS             <1..955
                     /locus_tag="PKL60_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNW95972.1"
                     /translation="NPDAKTFHFIGKDNIPFHAVIWPAELIGFRDGIDKAYGTQDPQP
                     LVLPYDVPANEFMNLEGRKISGSKNWAVWGLDFLERYDPDALRYYLTTAMPESRDTDW
                     DWEEFFQRNNNELVATWGNLVNRVLAFTYKHWEGVIPQPGELRESDLTLLATIRAGFT
                     GVSEKMERVQLRDALSEVMSLATEVNKYLDVHAPWFEIKTDKAAAAKSVFTAIQAIDW
                     LKLMFAPFIPFTSEALHQILGYQQPLFGSQSTRSVVDRLGAHEILEYRPAQSLSGSGN
                     DLWQPSDLMGGRPFHQPQPLVKKLDHSIVEEERARLRQSGS"
     gene            complement(952..1659)
                     /locus_tag="PKL60_07285"
     CDS             complement(952..1659)
                     /locus_tag="PKL60_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HNW95973.1"
                     /translation="MNATHPVIVLICANTEWKAVMGLLRPEECGSTPFGGIFAAQSGR
                     HNLLYAHSGWGKTATAAACQHLIDTRHPRLMINLGTCGGLAGRAQVGQILLAEETAMY
                     DIVESMSDYTTALERYRTRAELGWLPGSLPPGVCKARIVSADQDIQTQNFDLIADTFH
                     APAADWESAAFAWTAAKNQTPWLVLRGVSDLVSKETAETYQNVGLWRERTGRIMADLL
                     NLLPWFLDCFPSETETA"
     gene            complement(1669..2424)
                     /locus_tag="PKL60_07290"
     CDS             complement(1669..2424)
                     /locus_tag="PKL60_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNW95974.1"
                     /translation="MERFDRTGTEFIWNSSYGHMDATPEKQGAPFPDYLPLNLPETGL
                     IPLPDPARIELGHADLRQIIEQRRSVRRYDENAALSLEELSLLLWLTQGVKKLAEKTG
                     MSLRTVPSAGARHPFETFLAVRRVEGLENGVYQFIANKHALLPIISGEVILDQIGAAT
                     GNQAQVLTAAVTFIWTALPYRTSFRYGARAYRYLFLDAGHVCQNLYLAAESIGCGVCA
                     IGAYDDEAVNTALHLDGQEQFVIYMASLGKKAA"
     gene            complement(2851..4713)
                     /gene="uvrC"
                     /locus_tag="PKL60_07295"
     CDS             complement(2851..4713)
                     /gene="uvrC"
                     /locus_tag="PKL60_07295"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNW95975.1"
                     /translation="MEISEHVNSILSTLPDKPGCYLMKDAAGTIIYVGKAINLKNRVR
                     SYFHESADHSYKTRQMVRKIADIDIIVVNSELEALILEMNLIKEHRPFYNVRLKDDKR
                     YPYIKIHWADDFPKLTITRLMVEDGSRYFGPYTSVWAVHQTLDILRKIFPYLTCAREI
                     TGKDPRPCLYYDIKLCSGPCIGAIDQSSYRQMIDDLCKFLEGKTEPVVKRLQEEMQAA
                     SENLNYERAAALRDQIQSIEHVVERQKIISDNQKDTDIIALARTNGEACVQIFFVRSG
                     KLIGREYFILEGTEDEKNEAILKEFIKQFYSQAAFIPKRVLLPTEVEEARIIKEWLNT
                     RRSGEKIEIQVPKRGTNKSLVDMATENAVETLKALETRWKADKDRQHQALAELQAALN
                     LPEPPNRIECYDISNTMGTASVGSMVVFEQGVANKKLYRRFNIRSVEGPDDFASMEEV
                     LRRRFSHYHSAQAEKELPGKTPDLSFSILPDLLIVDGGKGQLGRALKVINEQGLDGRF
                     ALVGLAKQEEELFLPGQSDSVRLEERSQGLYLIQRIRDEAHRFAITAHRSRRGKIGLM
                     SRLDTIPGLGPARRKELILRFGSIEAILAAKPEEIAGIKGINLELARQIKAQLE"
     gene            4794..6524
                     /gene="mutL"
                     /locus_tag="PKL60_07300"
     CDS             4794..6524
                     /gene="mutL"
                     /locus_tag="PKL60_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNW95976.1"
                     /translation="MTIRILPETIASQIAAGEVVERPASAVKELIENAIDAGATAIAI
                     ETSEAGKRLIAVADNGSGIPADELPLALARHATSKLQSAEDLFEIHTLGFRGEALASI
                     GSVSRLTLTSKPEAQLAGYRIRVDGGLMGPSGSVPAPTGSEVRVEDLFFNVPARMKFL
                     KSDSTENRQIIGLITRYALAYPHIRWSLTQDQRAAFKTTGSGSQMEILQELFGAQDSK
                     QLLPIAFSEREIGINGFVSSLSLNRSNRKDITFFVNGRWVQDAALTAAVIKAYNTLLM
                     VGRYPVAVLFIELPAKQVDVNVHPSKAEVRFRDADQVFSALQRAIRRGLLAYSPVPEI
                     NPVSLWGRQTGAETGSADFAPASAAAVWGGVDAAPEAARPQSYLPMERLPLLRLIGQV
                     AATYLIAEGPDGLYLIDQHAAHERVLFDQLMAQYHAAAIPVQALVQPAAVEFPAEKAQ
                     TLAQQLPVLRELGFEVEEFGPNAFLVRAVPAVFAAGDPKAVLQALVEDFEEDEEPLAA
                     EIEAKIAGRVCKRLAVKAGKILSESEQKALLLNLEASQSPRTCPHGRPTMIHLSAALL
                     ERQFGRTGAI"
BASE COUNT         1379 a         1834 c         1992 g         1489 t
ORIGIN      
        1 caaccctgac gcgaaaactt ttcatttcat cggcaaggac aatataccct tccacgcggt
       61 catctggccc gcagaactca tcggattccg cgacggcatt gacaaggctt acggcacaca
      121 agaccctcag ccgctggtgc tgccctatga tgtgccggcg aatgagttta tgaacctgga
      181 aggacgcaaa atcagcggca gcaagaactg ggcggtttgg ggattggatt tcctcgagcg
      241 ctacgacccg gacgcgctgc gctactatct gacaaccgcc atgcccgaaa gccgggatac
      301 cgactgggac tgggaagagt ttttccagcg caacaacaac gaactggttg ccacctgggg
      361 caacctggtg aaccgcgttt tggcgttcac ttacaaacat tgggagggcg tcattccgca
      421 gcctggtgaa ctgcgcgaaa gcgacctcac gctgctggcg acaatccggg cgggctttac
      481 gggcgttagt gagaagatgg agcgtgtgca gctgcgcgac gcgctctcgg aagtgatgag
      541 cctggccacc gaggtcaaca aatacctgga cgtgcacgcg ccctggttcg aaatcaagac
      601 cgacaaagcc gcagcagcca aatccgtctt taccgccatt caggccatcg actggctgaa
      661 actgatgttt gcccctttta tcccgtttac ctccgaagcg ctgcaccaga ttctgggtta
      721 ccagcagccg ttgttcggca gccagagcac gcgcagcgtg gtcgaccggc taggagcgca
      781 cgaaattctg gaatatcgac ccgcgcagtc cctgagcggc agtgggaacg acttatggca
      841 gccctcggat ctgatgggcg ggaggccgtt ccaccaaccc cagccgctgg tgaagaaact
      901 ggaccacagc attgtagagg aagaacgcgc ccgcctgcgg cagagcggta gttaggctgt
      961 ctctgtttcc gaagggaagc agtcgaggaa ccatgggagc aggttcaaca ggtcggccat
     1021 gatgcggcct gtgcgctcgc gccagagccc cacattttga taggtttccg ccgtttcttt
     1081 gctgaccaga tcactgacgc cgcgcaggac aagccatggc gtctgatttt tcgcggcagt
     1141 ccaggcgaaa gctgctgatt cccagtccgc ggctggcgcg tgaaaagtat ccgcgataag
     1201 gtcaaaattc tgcgtctgga tatcctggtc ggcggaaaca atgcgcgctt tgcacacgcc
     1261 tggggggagg gagccgggaa gccagcccag ttccgcgcga gtgcggtagc gctccagcgc
     1321 ggtggtgtaa tccgacatac tttccacgat atcgtacatt gccgtttcct ccgccagaag
     1381 gatttggccg acttgagcgc gcccggccaa tcctccgcag gtgccgaggt taatcatcaa
     1441 gcggggatgg cgagtgtcta ttaagtgttg gcaggctgcc gcggtcgcgg ttttccccca
     1501 gccgctgtgc gcatagagca ggttgtgccg accagactgc gccgcgaata tccccccgaa
     1561 gggtgtagag ccgcattctt ccggacgcag cagccccatc accgccttcc attccgtgtt
     1621 cgcgcaaatc aggacaataa cgggatgtgt ggcgttcata gcgctggatc aagcggcttt
     1681 tttgcccagg gaagccatat aaatgacgaa ttgttcctgg ccgtccagat ggagggcagt
     1741 gtttacggct tcgtcgtcgt atgcgccaat cgcgcaaact ccgcagccga tactttctgc
     1801 tgccagatac aggttctgac agacatgccc ggcgtccaga aaaaggtagc ggtaggcacg
     1861 cgcgccgtag cgaaagctcg tgcggtaggg cagggcagtc cagatgaagg tcacggcggc
     1921 tgtcaggacc tgggcctgat tgccggtcgc cgcgccgatt tggtcaagga ttacctcacc
     1981 tgaaatgatg ggaaggagcg cgtgcttgtt cgcgatgaac tgatagaccc cattctcaag
     2041 gccttccacg cgccggactg ccagaaaggt ttcgaagggg tgtcgcgcgc cggcggaggg
     2101 tacggtgcgc agcgacatgc ccgtcttttc cgcgagcttc ttcacgccct gagtgagcca
     2161 cagcagcagg gataattctt ccaggcttag cgccgcgttc tcatcgtagc gccttaccga
     2221 gcggcgctgt tcgatgattt ggcgcaggtc ggcatgcccg agttcgattc gggctggatc
     2281 aggaagcgga atcaggccag tttcaggcag atttaaaggc aaatagtcgg ggaaaggagc
     2341 gccttgcttt tccggtgtcg catccatgtg tccgtaagag gaattccaaa taaattccgt
     2401 gccggtcctg tcaaatcttt ccatagtaac ctgccatgcg cgctcgtcaa gaataaccca
     2461 acgagttagc gcaccaatat ccttgcatta aatactcttt ttgctgtgtc aggttttgtc
     2521 ctgtaattcc cagtcttccg ggacggtctt tgcccaggta attgatccgc caatcgcaac
     2581 ctggattgct tgggctccct tgattgtgtt cgggtcaagg tcgtactggt tggctatgcc
     2641 ctgggctaat tcaatgatat cccgatctcc agtccatgtg ccgtccctgt attctgcttc
     2701 gagggtctaa ttcacatggt cctaactcgg taatactacg attctcatta aaaacgaacg
     2761 agcgacagga ccatagcaag gataatggtc agcgcgagga ctgccatgac gatgcgggaa
     2821 gtgtttgctt gggaatgttt ttttgccata ttactccaat tgcgctttga tttgacgcgc
     2881 cagttccagg ttgatgccct tgattcccgc gatttcctcc ggcttggcag ccaggatagc
     2941 ctcgatcgag ccgaaacgca ggataagttc ttttctccgc gctggcccga gcccgggaat
     3001 agtatccagg cgcgacatca gaccgatttt accacgccgg ctgcgatgcg cggtaattgc
     3061 gaagcggtgg gcttcatcgc ggatgcgttg aatgaggtag aggccttggg agcgctcttc
     3121 caggcgcacc gaatcgctct ggccgggcag gaagagctcc tcctcctgtt tggccagccc
     3181 gacgagcgcg aagcggccgt ccaatccctg ctcgttgatt acctttagcg cgcgcccgag
     3241 ctgacctttg cctccgtcca caatgagcag gtcgggcagg atgctaaagg ataagtcagg
     3301 ggtttttccg ggcagttctt tttcagcttg ggcgctgtgg taatggctga aacgccggcg
     3361 cagcacctct tccatcgagg caaaatcgtc agggccttcc acgctgcgga tgttaaagcg
     3421 ccggtagagc tttttgtttg ccacgccctg ctcgaacacc accatactgc ccacgctggc
     3481 ggtgcccatg gtgttcgaga tgtcgtaaca ttcaatccgg ttggggggct cgggcaggtt
     3541 cagcgcagct tgcagttccg cgagcgcctg gtgctgacgg tctttgtccg ctttccagcg
     3601 ggtctccagc gcttttaagg tttccacagc gttttcggtc gccatgtcta ccagactttt
     3661 attcgtgccg cgtttgggca cttggatttc gattttctcg ccactgcggc gcgtgttcag
     3721 ccattctttg atgatgcggg cttcctcaac ctcggtcggc agcagtacgc gcttggggat
     3781 gaaggcggcc tgtgagtaaa actgcttgat gaattccttc aggatagctt cgttcttctc
     3841 gtcctcggtg ccttccagga tgaagtactc gcgcccgatc agtttgccgg aacgcacaaa
     3901 gaaaatttgc acgcaggctt cgccgttggt acgcgccagc gcgataatat cagtgtcctt
     3961 ttgattatcg gaaataatct tttgccgctc gaccacatgt tcgatactct ggatttggtc
     4021 gcgcagggct gcggcgcgct cgtagttcag gttttcagag gctgcctgca tttcttcctg
     4081 caggcgcttc accaccggtt cggttttgcc ctccaggaat ttgcacaggt cgtcgatcat
     4141 ctggcggtag gaagactgat ctattgctcc gatacaaggc ccggagcaca gtttaatatc
     4201 atagtacagg caggggcggg ggtctttgcc ggtgatttcc cgggcgcaag tcagataggg
     4261 gaaaattttc cgcaggatat ccagcgtctg gtgcactgcc caaacggagg tatacgggcc
     4321 aaagtagcgc gagccgtctt ccaccatcag gcgggtaata gtcagtttcg ggaagtcatc
     4381 cgcccagtgg attttgatgt aagggtagcg cttatcatcc ttgaggcgca cattataaaa
     4441 ggggcggtgt tccttgatca ggttcatttc aaggatcagc gcttccagct ccgaatttac
     4501 cacgatgatg tcgatatccg cgattttgcg caccatctgc cgggtcttgt aggaatggtc
     4561 ggcggactcg tggaagtagg agcgcacgcg gtttttcagg ttgatggctt tcccgacgta
     4621 gatgatagta cccgcggcgt ctttcatcag gtagcagccg ggtttatctg gcagggtgct
     4681 caggatactg ttaacgtgtt cagaaatttc catgaccagt ctattttaac tgaaatatca
     4741 accccggaat tcaagaggtt ctgcgaaaag gtataattcc ggcaggagaa aagatgacca
     4801 tcagaattct tcctgaaacg attgcttccc agattgccgc gggtgaggtg gtggaacggc
     4861 cagcttccgc ggttaaggaa ttaatcgaaa acgcgattga cgctggcgcc accgcgattg
     4921 cgatagaaac gagcgaggct ggcaagcgtt tgattgcggt ggcggataac ggctcgggta
     4981 tcccggcgga cgagcttccg ctcgctttgg ccaggcacgc caccagtaaa ctgcagtccg
     5041 ctgaggattt gttcgaaatc cacacgctcg gcttccgcgg agaggcgctg gcctccatcg
     5101 gttccgtctc gcgcctgacg ctgacctcca aacccgaagc gcagctggcc ggctaccgca
     5161 tccgcgtaga cggcgggctt atgggtccat cgggttcggt gcccgcgccc actggcagcg
     5221 aggtgcgggt tgaagatttg tttttcaatg tgcctgcccg catgaaattc ctgaagtcgg
     5281 acagcacgga aaaccgtcag ataattggtc tgataacccg ctacgcgctg gcttatccgc
     5341 acatccgttg gagccttacc caggaccaac gtgcggcgtt caagactaca ggcagcggca
     5401 gccagatgga aatcctgcaa gagctatttg gcgcgcaaga cagcaaacag cttctgccga
     5461 ttgccttttc agaacgggaa attggcatca acggctttgt cagcagcctt tcgctgaatc
     5521 gttcgaaccg caaagatatc acattttttg ttaacggacg ctgggtgcag gacgcggcgc
     5581 tgaccgcggc ggtaattaaa gcctacaata ccctgttgat ggtcgggcgc taccctgtcg
     5641 cagtgctgtt tatcgaactg ccggccaaac aggtggacgt aaacgtgcat ccctccaagg
     5701 cggaagtacg ttttcgggat gctgaccagg ttttcagcgc gctgcagcgg gcgattcggc
     5761 gcggactgct ggcgtattcc ccggttcctg aaatcaatcc ggtgagtttg tggggcaggc
     5821 aaacaggggc tgagaccggc agcgcggatt tcgcccccgc ttcagcagcc gctgtgtggg
     5881 gaggcgtgga tgcagcgccg gaagcggccc gcccgcaaag ctatctgccg atggaacgcc
     5941 tgccgcttct gcgcctgatc gggcaggtgg cggccactta cctgattgca gaaggacccg
     6001 acggcttgta cctgattgac cagcacgccg cgcacgaacg cgtgctcttc gaccagctga
     6061 tggcgcagta ccacgctgcg gcaattccag tgcaggcgct ggtccaaccg gctgcagtgg
     6121 aattcccggc ggaaaaggcg cagaccctgg cgcagcagct gcccgtcctg agggaattgg
     6181 gctttgaggt tgaagaattt ggcccgaacg cgtttctcgt gcgggctgtg ccggctgttt
     6241 ttgcggcagg cgacccgaaa gcggtgctcc aggcgctggt ggaggatttt gaggaggacg
     6301 aggaacccct cgctgcggaa attgaggcaa aaatcgctgg gcgagtatgc aagcgtctgg
     6361 cggtcaaagc cggcaagatc ctttcagaga gcgagcaaaa agcgctgttg ctgaacctgg
     6421 aagccagcca atcgccgcgc acctgcccgc acggtaggcc gaccatgata cacctgagcg
     6481 ccgcgctgct ggaaaggcag ttcggtcgga ctggcgcgat ataggatatg gggaatatct
     6541 gcctggataa gtgatgaagc ttcgctatgg gggaataatg agtaaagatt tgaaggattt
     6601 gtgcccgatt tttgacttgc cggaaaaacc agaaatggta taataccaat cctgcggggc
     6661 gtggctcagc ccggatagag cgcacggttc ggga
//
LOCUS       DAOQAD010000097         6649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_14092_length_6649_cov_4.781620, whole genome shotgun sequence.
ACCESSION   DAOQAD010000097 DAOQAD010000000
VERSION     DAOQAD010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14092_length_6649_cov_4.781620"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..814
                     /locus_tag="PKL60_07305"
     CDS             68..814
                     /locus_tag="PKL60_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNW95977.1"
                     /translation="MITLGIDIGGSATKGALVDTETGKMVSERVRFETEAPVKPREVA
                     ALVEKIRKKLGYHGIIGAGYPGVVKKGVAMTAANLHKNWIGTDVAALIYNQTGQITTV
                     LNDADAAGLAEMRFGVEEARLFKTVLFLTVGTGIGSALFVDGHLLPNTELGHLKIRGK
                     DAEHRASDAVRQREGLSWKKWGKAFTELLQTYEFLFNPDLFILGGGISSKYESYAEFL
                     KIDTKILPAKLENTAGIIGAGMAAQESSVL"
     gene            complement(817..1230)
                     /locus_tag="PKL60_07310"
     CDS             complement(817..1230)
                     /locus_tag="PKL60_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNW95978.1"
                     /translation="MAYVITNLCLRDGGCVVACPVACIVPGKPEEQYPLYYIDPNTCI
                     DCGACIPECPYGAIFTLHDVPQAFTAAGGEVLSAPVGTPGYENPLDTFTYEGVPVHIP
                     ATRVLAPGEIIDLTEFIRINRDFFENGPGFEALLF"
     gene            1290..2225
                     /locus_tag="PKL60_07315"
     CDS             1290..2225
                     /locus_tag="PKL60_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNW95979.1"
                     /translation="MPQPKRVPSIVLLVFSILAVSTAAILIRLVNDQASALVIAAYRL
                     SFSALLSGALYLTGKHDTKNLNRRNLMWMALSGAFLAAHFAAWISSLELISVSSSVIL
                     VTTTPLWVALLSPLVLKEKVRPVFYVGMAFALLGGLTIALSSSCTAAGSGLVCAPGPL
                     GGSPQTLKGMFLALLGAWMAAGYMLIGRKIRAQTDNSQYTLIVYASAALILVFASVFR
                     GEKLFGYSPRVYIVFLAMALIPQLLGHSVLNYALKFLPATLISMALLGEPIGSTILAV
                     LFLKEIPSTMEIIGGILILIGITVSVLPSKPANAA"
     gene            complement(2212..3774)
                     /gene="malQ"
                     /locus_tag="PKL60_07320"
     CDS             complement(2212..3774)
                     /gene="malQ"
                     /locus_tag="PKL60_07320"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNW95980.1"
                     /translation="MQTTRFSGVLLHPTSFPGPDGIGDLGPEAYRWVDFLKGSGCQMW
                     QILPLGPTGYGDSPYQCFSAFAGNPFLVSPLLLLEDGLLSLEDLRDRPRFPLERVDFG
                     PAIIWKNTILDRTYQNFQLLTRPSHLVDDFESFRAENRAWLEDYSLFMAIKESQNGEP
                     WNAWPRALKFREEAALAKFAYEHSSRIEKHQFSQFLFFRQWKALRQYAHEQGIQIIGD
                     IPFVIAQDSADVWADPKLFLLDAELNPTVVAGVPPDYFSADGQLWGNPLYDWPVHKAS
                     GYQWWKDRMASVLGLVDRIRLDHFRGFAAAWNVPFGNLTAREGHWEKGPGIELFEELQ
                     RSFPNLPIIAEDLGVITPDVEEMRDKFELPGMKILQFAFTGDPEDDFLPHHYPVNCFA
                     YTGSHDNNTAKGWYTQASAREQDFCRRYLNVSGEDISWSMIRAIWQSVARYVVAPMQD
                     LLSLGAEARMNLPGSQSGNWSWRMLPNALTEPLKQRLWEINLLYSRLSAPEKERYTAK
                     LNAELNGVVKPH"
     gene            complement(3779..5245)
                     /locus_tag="PKL60_07325"
     CDS             complement(3779..5245)
                     /locus_tag="PKL60_07325"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNW95981.1"
                     /translation="MSAQPSIRVLFASAEASPLAKVGGLGDVAGALPAALKEASSSAL
                     DIRVFLPFHAEIRQKNPPLGALGTFEVPYQAGSFPVELYVTALQGIPVYLLDSELFNH
                     NSPIYHGDPALDGRKYAAFSIALLEAARFLHWQPHILHANDWHTALAVTALKTLYQDD
                     PFLADTRSLLTIHNLPFNGYGSESAMTELGFPAGTDPDLPDWALLTPLPLGISAADRV
                     VTVSPSYAHEIQTLEFGCGLHEYLQKNNRKLTGILNGIDTKVWDPSVDPFIPFRFNLS
                     DLSGKAQNKAALQRELKLEVNPEIPLLTVVSRFDYQKGIGTILDAIPGNVRKSWQMVL
                     LGTGAPDLEARAADLMRQFPAKVVSLLRHDEAAAHKLYAAGDLFLMPSLYEPCGLSQM
                     IAMRYGNIPVARATGGLQDSIIDFHKDPSRATGFLFTEKTYEGLVNSLNSAIMVFHDK
                     PAWAQLIGNAMRNNFSWNSSAQNYWQIYQELTNSLTKR"
     gene            complement(5253..>6649)
                     /locus_tag="PKL60_07330"
     CDS             complement(5253..>6649)
                     /locus_tag="PKL60_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNW95982.1"
                     /translation="ALCDMAKKYDVPVHFHVSETLSEVENLRQEQGMPVIPYVRKFGM
                     LETKLIAAHCVHVDEGEIRSMQHAGAGIAHNPSSNLKLASGFAPIARMLELNANVGIG
                     TDGTASNNDLDMIDEIRLASFVAKTVAGDPTALPARQALAMATSIGAKALHIADITGS
                     LEKGKRADLILLDISPLHNQPRFRRDSENIYAQIIYAAKSTDITDVMVDGRWLMRERE
                     LLTLDEKELIRQARLVAEDIDRFLKNREESVLSKLIAIGGAAEEESFEVQAKVRVENL
                     EPILAALESPEIQIVRTRHYHEYDSYFTFEDASQGRLRYREDEFIGKNGEITSVRSRL
                     TLLGEPESALQNVHDGPFANEKIMLSRSRYLAPAVHSLRFYNEYFKPSELVEIEKNRL
                     RYLVKFRDTEFFINLDKITEPKLGKFVEIKSRTWSRQDAQNKTLLMRELLKTLGLGSL
                     PLVTKDYIHLVEEL"
BASE COUNT         1475 a         1788 c         1870 g         1516 t
ORIGIN      
        1 gcacgtcttt tcgattacat ttgaagaaca tcttaacaat gcctgtccct aattgctcgg
       61 aggaagaatg attacattag gtattgatat tggcggctct gccacaaaag gagccttggt
      121 tgataccgag actggaaaga tggtttctga acgggtccgc tttgaaacag aagcgccggt
      181 caagccaaga gaagttgccg cactggtgga gaaaatccgg aagaagctgg gttatcacgg
      241 catcatcggt gccgggtatc ccggcgtcgt aaaaaagggc gtggcgatga ccgcggcgaa
      301 cctgcacaag aattggattg gcaccgacgt cgcggccctg atttacaacc agaccggtca
      361 gattactacg gttttaaacg acgctgacgc agctggcctg gcagagatgc gtttcggggt
      421 ggaagaagcc cggctcttca aaaccgtact ctttcttacg gtcggaacag gcatcggctc
      481 cgcgcttttc gtggacggac atctgctgcc caatactgag cttggtcatc tcaaaatccg
      541 tgggaaggac gccgagcacc gtgcttccga cgcggtcagg caaagggaag gcttgagctg
      601 gaaaaaatgg ggaaaggctt tcactgagct gctgcagact tacgagttcc tgtttaaccc
      661 ggaccttttc atcctgggcg gcggaatcag ctcaaaatat gagagctatg cggaattcct
      721 gaaaattgac accaagattc tgcctgccaa gctggaaaat accgctggga ttatcggcgc
      781 ggggatggca gcccaggaat cgtcagtttt gtaagctcag aaaagcagcg cttcgaaacc
      841 aggcccgttt tcgaagaaat cccggttaat ccggatgaat tcggttaaat cgatgatttc
      901 tccgggggcg aggacgcgcg tggccggaat gtgcaccggt acgccttcgt aggtaaaggt
      961 gtccaggggg ttttcatacc cgggcgtccc caccggcgcg ctgagcacct caccgccggc
     1021 cgcggtaaag gcctgcggca cgtcgtgcag ggtaaaaata gcgccgtacg gacactcagg
     1081 aatgcatgcc ccacaatcga tgcaagtgtt gggatcgatg tagtaaagcg gatattgttc
     1141 ctcaggtttt ccaggaacaa tacaggcgac cgggcaggca accacgcacc cgccgtcccg
     1201 caaacataaa ttggtaatca catacgccat atttcactcc agcggtaaaa tcatcgcatt
     1261 agtttaacac agttccaaaa ggttaccagg tgcctcaacc caagcgcgta ccttctattg
     1321 ttctgctggt cttcagtatc ctggctgttt ccacagccgc aatcctcatt cgcctggtga
     1381 atgaccaggc ttcggcgctt gttattgctg cgtaccgcct cagtttctca gcgctgctta
     1441 gcggagcgtt gtacctcact ggcaagcatg acacgaagaa cttgaatcgg cgcaacttga
     1501 tgtggatggc gctctccgga gctttcctgg ccgcgcattt cgcagcctgg atcagctcgc
     1561 tggaattaat cagtgtcagc agctcggtaa tcctggtcac caccacgcca ctgtgggtcg
     1621 ctttgctctc tcctctggtg ctcaaagaga aagtccggcc ggtgttttat gtggggatgg
     1681 cttttgcctt gctagggggc cttaccatcg cgctcagcag ctcctgcact gccgcaggct
     1741 ccgggctggt ttgcgcgccg ggcccattgg gcggcagccc gcaaacgctg aagggcatgt
     1801 ttttggcgct gctgggggca tggatggctg cagggtatat gttgattggc cggaaaattc
     1861 gtgcgcaaac tgacaattcc cagtacacgc tgattgttta cgcgagcgct gcattaatcc
     1921 tcgtttttgc gtctgttttt cgcggtgaga agctttttgg gtactctcca cgcgtatata
     1981 tcgtttttct ggctatggcg ctcatccccc agttgctcgg gcactcggtt ttgaactatg
     2041 ccttgaaatt cctccctgcc acgctgattt cgatggcgct tctgggcgaa ccgattggat
     2101 cgaccattct ggcagtcctt ttcttaaaag aaataccgtc aacgatggaa atcattggcg
     2161 gtatcctgat tttaataggt attacggtct cggtgctgcc ttcaaaaccc gctaatgcgg
     2221 cttaaccaca ccgttgagct cggcattcag cttggcggtg tagcgttctt tttccggcgc
     2281 ggataggcgg ctgtaaagta agtttatttc ccacaggcgc tgctttaagg gctcggtcag
     2341 tgcgttgggc agcatccgcc aggaccagtt tccgctctgt gagcccggca gattcatgcg
     2401 agcttcggcg cccagggaaa gcaagtcttg cattggcgcg actacgtacc gcgcgacgga
     2461 ctgccagata gcccggatca tggaccagga gatatcctca ccggaaacgt tcagatagcg
     2521 gcggcaaaaa tcctgttcgc gcgcggaagc ctgcgtgtac caacctttcg cggtgttgtt
     2581 atcgtgcgaa ccggtgtacg cgaaacagtt cacggggtag tggtgcggca gaaagtcgtc
     2641 ttcggggtcc ccggtaaagg caaactgcag gattttcatg cccggcagct cgaatttgtc
     2701 gcgcatttct tccacatccg gggtgataac gcccaggtct tcagcaatga tgggcaaatt
     2761 cgggaaagag cgctgcagct cctcaaacag ctcaattccg ggtccctttt cccaatgccc
     2821 ctcgcgggca gtgagattgc caaagggcac attccaggcc gcggcaaagc cccggaagtg
     2881 gtccagccgg atccggtcga ccagacccag gacggatgcc atgcggtctt tccaccattg
     2941 atagccgctg gctttgtgta ccggccaatc gtagagtgga ttaccccaca gctgaccgtc
     3001 agcagaaaaa taatccggcg ggacgcccgc gacgacggtg ggattaagct cggcatccag
     3061 caggaaaagc ttcgggtccg cccaaacgtc agcgctgtcc tgcgcaatga caaaggggat
     3121 gtcgccgata atctggattc cttgttcgtg ggcgtactgg cgcagcgctt tccattggcg
     3181 gaagaagagg aactggctga actgatgctt ttcgatccgg gaagaatgct cgtaggcaaa
     3241 ctttgccagc gctgcctctt cgcggaattt caaagctctg ggccaggcgt tccacggctc
     3301 gccattctga ctttctttaa tcgccataaa gaggctgtaa tcttccagcc aggcccggtt
     3361 ttcggcgcgg aagctctcaa agtcatccac caaatgcgaa ggcctggtga gcagctgaaa
     3421 attctgataa gtccggtcga gaatggtgtt tttccaaatg atggcggggc cgaaatcaac
     3481 ccgttcgagc gggaacctgg gacggtccct caggtcctcc agcgacagca atccgtcctc
     3541 cagcagcagg agcgggctta caagaaaggg gttgccggcg aacgctgaaa aacactggta
     3601 gggagagtcc ccatatccgg tcggtccaag cggcagaatc tgccacatct ggcagccgct
     3661 gcctttgagg aagtcaaccc aacgataagc ttcggggccc aaatcgccga tgccgtccgg
     3721 tcccggaaag ctggtagggt ggagaagcac gccagaaaaa cgagtagtct gcataccatt
     3781 acctctttgt caatgagttc gtcagttcct gatatatttg ccagtaattc tgagcggagg
     3841 aattccagga gaaattattc cgcattgcat tcccaatgag ctgcgcccag gccggcttat
     3901 cgtggaaaac cataattgcc gagtttagcg agtttaccaa gccctcgtag gttttttcgg
     3961 tgaacaagaa accggttgcc cttgacgggt ctttgtggaa gtcgataatg ctgtcttgca
     4021 ggccgccggt ggcgcgcgca accggaatat tcccgtagcg catcgcaatc atttgtgaga
     4081 gtccacaggg ctcgtacagg gacggcatca ggaagaggtc ccccgctgcg tatagtttat
     4141 gcgccgcggc ctcatcatgt ctcagcagac tgacaacctt cgcgggaaat tggcgcatca
     4201 ggtcggcagc tctggcttcc aggtcaggag cgccggtacc cagaagcacc atctgccagg
     4261 atttccgcac attcccggga atcgcgtcga gaatcgttcc aattcctttt tggtaatcaa
     4321 agcggctgac cacggtgagc agggggattt ccggattgac ttccagcttc agttcacgct
     4381 gtaaggcagc tttattttgg gctttcccgc tcaggtcgga aagattgaaa cgaaacggga
     4441 tgaacgggtc gacagatggg tcccaaacct tggtgtcaat accgttcaaa atccccgtga
     4501 gtttccgatt gtttttctgc agatattcgt gcagcccgca gccaaattcg agtgtctgga
     4561 tttcgtgggc ataactcggc gaaacggtca caacacggtc cgccgcggat atgccaagcg
     4621 gcaaaggggt gagcagagcc cagtccggca ggtcaggatc agtccctgca gggaaaccga
     4681 gctcagtcat cgcggattcc gagccgtacc cattgaacgg caggttgtga atggtcagca
     4741 gactcctggt atcagccagg aacgggtcgt cctgatacag ggtttttagg gcggtaacag
     4801 ccagggcggt atgccagtcg ttcgcgtgaa gaatatgagg ctgccaatgc agaaagcgcg
     4861 cggcttccaa cagggcgatg gaaaaagcag catattttcg accgtccagc gctgggtccc
     4921 catggtaaat cggggaattg tggttgaata attcgctatc cagcaggtaa accggaatac
     4981 cctgcaacgc tgtcacgtac agctctactg ggaatgaacc agcctgatag ggtacctcaa
     5041 acgtacccag ggcgccgagc ggcgggtttt tttgcctgat ttcggcatgg aagggtaaaa
     5101 aaacacggat atccagcgcg ctggaggatg cttcctttaa agctgcgggc agcgccccgg
     5161 caacgtcgcc caatcccccg actttagcca aaggggaggc ttcggcgctg gcaaacagga
     5221 cccggatgct tggctgcgcg ctcatcgcga acctaaagtt cttctaccag atggatgtaa
     5281 tctttggtga ccagcgggag ggaacccaag cccagggttt tcaacagttc gcgcatgagc
     5341 agtgtcttgt tctgcgcgtc ctgccggctc caggtgcggc tcttaatctc cacaaatttg
     5401 ccaagctttg gttcggtaat cttatccagg ttaataaaaa actccgtgtc gcggaatttc
     5461 accagatatc tcaggcggtt tttctcaatc tcgaccagct cgctgggttt aaagtactcg
     5521 ttatagaagc gcaggctgtg caccgcggga gcaaggtaac gcgaacgcga gagcataatc
     5581 ttttcgttag caaaaggtcc atcgtgtacg ttctgcagtg cgctctcagg ctcgccaagc
     5641 agtgtcaggc gagaacgcac gctggtgatc tcaccattct ttccgataaa ctcatcttcc
     5701 cgatagcgca gtcttccttg cgaagcatct tcaaaagtga agtacgagtc gtattcgtgg
     5761 tagtggcggg tacgcacaat ctgaatttcc gggctttcga gcgcggccag aatcggttcc
     5821 aggttttcga cccggacttt tgcctgaact tcaaagcttt cttcctccgc cgcgccgccg
     5881 atggcaatca gcttggagag cacggattct tcacggttct tcaggaagcg gtcgatatcc
     5941 tcggcaacga gacgagcctg gcggattaac tccttttcgt ccaatgtcag gagctcgcgc
     6001 tcacgcatca gccaacgtcc atcgaccatc acatcggtta tatccgtgga tttggcggcg
     6061 tagataattt gcgcatagat attttcagaa tcccgacgaa aacgcggctg gttgtgcaaa
     6121 ggggaaatat ccagcaaaat caggtcagcg cgtttcccct tttcaaggct gccggtgata
     6181 tccgcgatgt gcagcgcttt cgcgccaatg gatgtagcca tagccaaagc ctgccgcgct
     6241 ggcagcgcag tagggtcgcc agccacggtc ttggcgacga agctcgccag ccgaatctcg
     6301 tcaatcatgt ccaggtcgtt gttggaagct gtgccgtctg taccaatgcc gacgttcgcg
     6361 tttagctcca gcatgcgcgc gataggggca aatccggaag caagctttag attggaggag
     6421 ggattgtggg cgatgccggc gcctgcgtgc tgcatgctgc ggatttcgcc ttcgtccaca
     6481 tgcacgcaat gggcagcaat cagtttggtt tccagcatcc cgaacttccg cacatatggg
     6541 ataaccggca taccctgttc ctgacgcagg ttctctacct ccgagagcgt ctccgacaca
     6601 tggaaatgca caggcacgtc gtactttttc gccatgtcgc acaaggcag
//
LOCUS       DAOQAD010000098         6596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_14244_length_6596_cov_7.285889, whole genome shotgun sequence.
ACCESSION   DAOQAD010000098 DAOQAD010000000
VERSION     DAOQAD010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14244_length_6596_cov_7.285889"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..1284)
                     /locus_tag="PKL60_07335"
     CDS             complement(349..1284)
                     /locus_tag="PKL60_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95983.1"
                     /translation="MKTIKTTKLLALSLLISALLLGACQAGGTAEPVKTTPQAAPLAI
                     PLDLEVKGTVFEVIQGGYSFEAPAGSDVSISGPGALISALDSSLLFSLNGINAMSTEK
                     SDEEILETLLETLFSGKQSSYKQSELMSSAVEGYEGAAYDFTGKFLGSQVEGRALVIK
                     PDAKRYVSMIGMALVEEDPELWKTTGKDAFNFLLGRYQILPADRLAIADLCPISPNAD
                     YGMSPDNAVKVGGGLLSGPLRERAYLDNLLGSTGEPVTYVWARSVETPDSIVDEYTLT
                     VGKETITLYLDQYAFGVINAPRGLGCMGAFPISEP"
     gene            complement(1295..3043)
                     /locus_tag="PKL60_07340"
     CDS             complement(1295..3043)
                     /locus_tag="PKL60_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNW95984.1"
                     /translation="MRQTLLTNDQLLHLQREKEVLNEAQSRLSAFELPQEALDALEKA
                     ARQLDELFLIVVVGEFNSGKSALINALLGERVLKEGVTPTTARVTLVRWGEQPGEQIV
                     DEGFAIFTYPLEMLKELNIVDSPGTNAIIRRHEILTNEYVPRSDLVLFTTSADRPLTE
                     SERLFLEKILSWGKKIVLVVNKADILENREAVEEVRTFVSGHTAGILGAEPQLFVVSA
                     KLGQKALLAETQEQKALLRTESGMDALETYITETLDDRARLGLKLKNPLGVAEKVLRQ
                     AQELNQAQAQELEADSQLSADLEGMLETYSKALQSETAPRLAEVENVLQRFETRGQDF
                     FDDTLRLTNIANLTKSEKIKTKFEQDVLADVPQEIDEKVRAMIDWLVDKDLNVWYQVM
                     GALERRQAAARANLDSSHLSAQAERRKDLMESVGQTIRGIVNSYNRKKEAEELSSFVE
                     GAVAQTALFEVGAVGLGALVATALFSSAMDVTGIVAAGTLAILGLFVIPYKRKQVKQK
                     FKEKMEELRTNLMSTLSAAFSRESGLAIQRLNDKISPYTAAVRADQQKIRSDSESLSG
                     LDNLLKETRDAIQKVL"
     gene            complement(3147..3611)
                     /locus_tag="PKL60_07345"
     CDS             complement(3147..3611)
                     /locus_tag="PKL60_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNW95985.1"
                     /translation="MKEHVRNSVKAVIIIGGKILVLQKMDGLSLYSVLPGGGQKKGET
                     LPEALQRECLEEINAQVNVGELLFVRDYISDKHEFAASSAHVHQVEFLFACELLGDYQ
                     PGSGSEPDRGQQQVLWVPLDALAEANFYPRALLPYLRGPGAPNRPVYLGDVN"
     gene            complement(3631..4263)
                     /locus_tag="PKL60_07350"
     CDS             complement(3631..4263)
                     /locus_tag="PKL60_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95986.1"
                     /translation="MGAKLTPKVILEGTRLTFKTEIALRLNEHPRFVGPRKYRYHSPL
                     ISAEWCAFTNFPWGRGLINYQPEEEAQALETYRTWARLFELQRYYSWVVDRFHLSTQA
                     YQLQTYGKCPDFGWLEERLLPLNFRLIFLTRSPESFAQAREERLKVSGNPRQYDDLGI
                     FQREQELLESLVLRSRLPCLRVDITDNDLEAAVERVVAWMDASGGLSMPD"
     gene            complement(4395..6443)
                     /locus_tag="PKL60_07355"
     CDS             complement(4395..6443)
                     /locus_tag="PKL60_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95987.1"
                     /translation="MENNSPTKRIERCGLRAWGMENWLFAAGLALYLLTRFIQLDKFP
                     IFFFTDEAVQTMSAVDLIRRGFRDSAGRLFPVYFENGGQYNLSLSVYLQLLPALLPRS
                     VWLTRGVCALLTLGFPLSVGLALRDYFRARVWWLAPVFIAAVPAWFLHSRTAFETSLG
                     VSMFSLFLFFYLGYRLRNRKLLPLALIFGALAFYSYSPLQLVVVLSGAALLIADWRYH
                     FQDKRVLLRGLLTLLVAALPYALFRINHPDALAQHLTQLQSYWVSGASLGAKAGAYLR
                     NYLRGLDPRYWFFPNGADLIRHQMKGMGHFPLLSLPLVFLGIVQAVRGVRQPEKRAIL
                     IALLAAPGGAALVGPAITRLLVMVVPLSYLALLGTDFVLDWLRPGEIIRKLWLPVVCG
                     LCAASSLFITWDALRNGPTWFENYGMYGLQWGGQTLFDEVGSFMRANPDKEIILSPNW
                     ANGTDVVARFFLGDPLPIIVGDIAPYTLYQLPLDKNKVFVMPREEFEGLPKTGKFTDI
                     EVLKVLPYPNGKPGFYFVSLRYAEGIEEIFAEEQAERSRPKSAVVELLGQQVSVQYPL
                     LDINDIYQAFDGDDATLIRSFEANPLVIVLEFPEPVSLSRVSALVGNAASRLELELSP
                     ADGSGTLRYSAETAGFAQVSPLSIPFDAPVSVKALRVSLLSVNEGEPAHVHLWELVLE
                     "
BASE COUNT         1321 a         2041 c         1862 g         1372 t
ORIGIN      
        1 ccgacaagct ttggccggcg ggtttgtgca gccgtcatcg ggttcgctcc tgcgcttttg
       61 cacccagcat aaaccgctcc tcatcctggg cgccagcgaa ggatctggtt ctctcacaag
      121 agtcgtaggt cgggtggagc ggcagcgcaa cccgacaaaa cgtggtcttg ggtcgggcgg
      181 ggcggcagcc caatccgaca gaacgtggtc ttgggtcggg tggagcgcca gcgcaacccg
      241 acaagctttg gccggcgggt ttgtgcagcc gtcatcgggt tcgctcctgc gccgccgcac
      301 tccacctaac gctgcatcgg tttcccaacg accatcccgc atcgctgtct acggctcgct
      361 aatcggaaaa gcccccatgc agcccagccc gcgcggggcg ttgatgaccc caaacgcgta
      421 ctggtccaga tacagcgtga tcgtttcctt gccgaccgtc agggtgtatt cgtccacaat
      481 gctgtcgggc gtttccaccg agcgcgccca cacataagtc accggttcgc cagtggagcc
      541 aagcaggttg tccaggtaag cccgctcgcg cagcggaccg ctcagcaatc cgccgcctac
      601 cttcaccgca ttatccggag acatgccata atccgcgttc ggcgagattg ggcacaagtc
      661 agcgatagct aatctatcag caggcaggat ctggtagcgc ccgagcaaaa aattgaaagc
      721 gtctttgcca gttgttttcc atagctccgg gtcttcttcc accagcgcca tcccaatcat
      781 ggagacatag cgcttggcat ccggtttgat taccagcgcc cggccttcca cctgggaacc
      841 gagaaacttg cccgtaaaat cataggcagc cccttcgtaa ccctccaccg cagagctcat
      901 cagttcggac tgcttgtagc tgctttgctt gccgctgaaa agggtttcca gcagggtttc
      961 cagaatttcc tcgtcggact tttcggtgga cattgcgttg atgccgttga gtgagaaaag
     1021 cagcgacgaa tccagcgcgc tgatgagcgc gcccgggcca gaaatgctga catcactgcc
     1081 cgccggggct tcaaagctgt atccgccttg aatcacttca aaaacggtcc cctttacctc
     1141 caggtccagc gggattgcta atggcgcggc ttgcggggta gttttgaccg gctccgcggt
     1201 accgcccgcc tggcatgcgc caagcaggag cgcgctgatg agcaggctga gtgccaggag
     1261 ttttgttgtt ttaattgttt tcattgcggc gtatttacag gaccttttga attgcgtccc
     1321 gcgtctcctt caatagatta tccaacccgg aaaggctttc tgagtcggat ctgattttct
     1381 gctggtcagc tctcactgcc gcggtgtaag gcgatatttt atcgttcagc cgctgaattg
     1441 ccaacccgga ttcacggctg aatgccgcgg agagggtgct catcaggttc gtgcgcagtt
     1501 cttccatctt ttctttgaat ttctgcttga cctgcttgcg cttgtaggga attacgaaca
     1561 atcccagaat cgccagcgtg cccgctgcga caataccggt cacatccatc gcggaggaaa
     1621 agagggccgt ggcgaccagc gcgcccaagc ccaccgcgcc cacttcaaac aaggcggtct
     1681 gcgccaccgc gccttccacg aacgagctca gctcctccgc ttcttttttg cggttatagc
     1741 tgttcacaat gccgcggatg gtctgcccga cgctctccat caggtccttg cggcgctcgg
     1801 cttgcgcgct caggtgcgag ctgtccaaat tcgcgcgggc tgccgcttgc cgccgttcca
     1861 aggcgcccat cacctggtac cagacgttca ggtctttatc caccagccag tcaatcatgg
     1921 cgcgcacttt ttcgtcaatt tcctgcggca catccgccaa cacatcctgc tcgaatttcg
     1981 ttttgatttt ctcgcttttg gtcaggttgg cgatattggt caggcgcagc gtgtcgtcaa
     2041 aaaagtcctg cccgcgcgtc tcaaaacgct gcagcacgtt ttcgacctcc gccaggcgcg
     2101 gcgctgtttc ggactgcagc gccttgctgt aggtctcaag catgccttcc aggtccgcgg
     2161 acaattggct gtccgcctcc aactcttggg cttgcgcctg gttcagttcc tgggcctggc
     2221 gcagtacctt ttcggctacc cccagcgggt tcttcagctt caaacccagc ctggcacggt
     2281 cgtcgagcgt ttcggtgatg taggtttcga gcgcgtccat gccgctttcc gtgcgcagaa
     2341 gcgctttttg ttcctgcgtc tcagccagca gcgccttttg gcctaactta gcggagacga
     2401 cgaaaagctg cggttcggcg ccaaggatac cggccgtatg cccggaaaca aacgtgcgca
     2461 cttcctccac cgcttcccgg ttctcgagaa tatcggcttt gttcaccacc aacacaattt
     2521 tcttacccca ggagaggatt ttctccagga agaggcgctc actttcggtc agcggccggt
     2581 ccgccgaggt tgtaaacagc accaggtcgc tgcgtgggac atactcattg gtcaggattt
     2641 cgtgccggcg gatgatggca ttcgtaccag gggaatcgac aatgttcagt tccttcagca
     2701 tctccagcgg gtaggtgaaa atggcgaaac cctcatccac gatttgctcg ccaggctgct
     2761 cgccccaacg caccagcgtc acgcgggccg tggtaggcgt cacgccttcc ttgagaacgc
     2821 gttcacccag cagcgcgtta atcagcgcgc tcttgcccga gttaaactct cccaccacga
     2881 caatcaggaa cagctcgtcc aactggcgcg cggctttctc cagcgcgtcc agagcctcct
     2941 gcggcagctc aaaagcagac agccggctct gggcttcgtt cagcacctct ttttcgcgct
     3001 gcaagtgcag cagttggtca ttggtaagca gggtctggcg catggtggtc tccttactgg
     3061 cgcggcacat aaaaccgctg aactgaatta tacctttggc ttgctctgat gaaggctgtg
     3121 cgcaatccag cccgtttggc agcggctcaa ttgacatccc ccagatagac cggtcggttt
     3181 ggcgcgccgg ggccgcgcaa gtagggcagc agcgcgcgcg gataaaagtt cgcttcggcc
     3241 agcgcgtcca gcggcaccca cagcacctgc tgctgtccgc ggtctggttc gctgccgctg
     3301 ccgggttggt aatctcccag aagctcacag gcaaacagga attcaacctg gtgtacatgc
     3361 gcggagctcg cggcgaattc gtgtttgtcg ctgatgtaat cccgcacgaa aagcagctcc
     3421 cctacgttca cttgcgcgtt aatctcctcc aggcactcgc gctgcagcgc ttcgggcagc
     3481 gtttcgcctt ttttctggcc tccgccgggc agcacgctat acaggctgag cccgtccatc
     3541 ttttgcagga ccaggatttt tccgccgatg atgatgactg ccttgactga attcctgaca
     3601 tgttctttca tgtttaacct cataagcccc tcaatccggc atgctcaatc cgccgcttgc
     3661 gtccatccaa gccactacgc gttccaccgc tgcctccagg tcattatccg taatatccac
     3721 ccgcaggcag ggcagccggc tgcgcaaaac caggctctca agcagctcct gttcgcgctg
     3781 aaagattccc aggtcgtcat actggcgcgg attgccggag actttcaggc gttcttcgcg
     3841 cgcttgcgca aaagactccg ggctgcgggt gaggaatatc aagcggaagt tgagcggcag
     3901 caggcgctct tccagccagc caaagtcggg acatttgccg taggtctgca gctggtaggc
     3961 ctgcgtggag aggtggaaac ggtcaaccac ccaggaatag tagcgctgaa gttcgaacag
     4021 gcgcgcccag gtgcggtagg tttccagcgc ctgggcttct tcttccggtt gataatttat
     4081 caagccgcga ccccagggaa aattagtaaa cgcacaccat tccgcggaga tgagcggtga
     4141 gtggtagcgg tacttgcgcg ggccgacgaa acggggatgt tcgttcaagc gcaaggcgat
     4201 ttccgtcttg aaggtcaggc gcgtgccttc caggatgact ttgggggtta gtttagctcc
     4261 catggggctc ctttgtgttt cctgtcaagt ctatcttcca ccaaaaggta ttgcgcaaat
     4321 gccggacctc tgaacaggct tgcccgctct gcgctgtccg gttttcattt cactgcgctg
     4381 ggcctgaagc gcgctcattc cagcaccagt tcccacagat gcacatgcgc gggttcgcct
     4441 tcgttcacgc tcaacagcga aaccctgagc gctttcacgc tcactggcgc gtcaaagggg
     4501 atgctgagcg ggctgacctg cgcgaagccg gcagtctccg cgctgtagcg cagtgtcccg
     4561 ctgccgtccg cggggctcag ctcaagctcc agccggctgg cagcgttccc caccagcgcc
     4621 gaaacccggc tgaggctgac aggctctggg aactccagta caatgaccag cggattggct
     4681 tcgaagctgc ggatgagggt ggcgtcgtcc ccatcaaagg cctggtagat atcgttgatg
     4741 tccaacagcg ggtactgcac cgacacctgc tgccccagga gctccacgac tgcgcttttg
     4801 ggtcggctcc gctccgcttg ttcttccgcg aagatttcct caatgccctc cgcatagcgc
     4861 agggagacga agtaaaagcc tggtttccca ttggggtagg gcagcacctt aaggacctcg
     4921 atatcggtaa atttaccggt cttgggcagc ccctcaaact cttcccttgg cattacgaag
     4981 actttgtttt tgtccagggg cagctggtag agggtgtaag gcgcgatgtc ccccacgatg
     5041 ataggcagcg ggtcccccaa aaagaagcgc gcgacgacgt cggttccgtt cgcccagttc
     5101 gggctgagga taatctcctt gtccgggttc gcgcgcataa agctgccaac ctcgtcgaaa
     5161 agcgtctgcc cgccccactg cagaccatac atgccgtaat tctcgaacca ggtaggccca
     5221 ttgcgcagcg catcccaggt gatgaacagg ctggaggccg cgcacaaccc acagaccaca
     5281 ggcagccaaa gcttgcgaat aatctccccg ggccgcagcc agtcaagcac aaaatctgta
     5341 ccaagcagcg ccaggtagct gagcggcact accatcacca gcaagcgtgt aatggccggc
     5401 ccaaccagcg cggcgccgcc gggcgctgcc aaaagcgcaa tcaaaatcgc gcgcttttca
     5461 ggctggcgca ccccacggac ggcctgcaca attcccaaga acaccagcgg caggctaagc
     5521 aagggaaaat gacccatgcc cttcatttgg tgccggataa ggtccgcccc gttcgggaaa
     5581 aaccagtagc gcggatccag cccacgcagg tagttgcgca ggtacgcgcc tgcttttgcg
     5641 cccagggaag cgcccgacac ccagtaagac tgcagctgcg tcaggtgctg tgccagcgcg
     5701 tcgggatgat tgatccggaa gagcgcgtag ggcagcgccg cgaccaggag ggtaagcagc
     5761 ccgcggagca ggacgcgctt gtcctgaaag tggtagcgcc aatccgcgat gagcagcgcg
     5821 gcgccgctga gcactactac cagctgcagc gggctgtagg aataaaatgc cagcgccccg
     5881 aaaatgagcg cgagcggcag cagcttgcgg ttcctcagcc tgtagccgag gtagaagaaa
     5941 agaaacaggc tgaacatgga tacgcccagg ctggtttcaa aggccgtgcg cgaatgcagg
     6001 aaccaggccg gcacagcggc gataaacacc ggcgccagcc accagacccg cgctctgaaa
     6061 taatcccgta aggcaagccc cacgctgagc gggaaaccga gtgtgagcag cgcgcaaacc
     6121 ccgcgcgtga gccatacgga gcgcgggagc agcgccggca gcagctgcaa gtacacagac
     6181 aggctgaggt tgtattgccc gccgttctca aagtacactg ggaacaggcg tccggcgctg
     6241 tccctgaacc cgcgcctgat aaggtctacc gcggacatcg tctgtacggc ttcgtcggtg
     6301 aagaagaaaa tcgggaattt atccagctgg atgaagcgcg tcagcaggta gagcgccaga
     6361 ccggcggcaa acagccagtt ttccattccc caggcgcgca agccgcagcg ctctattcgt
     6421 ttcgtcgggg aattgttctc cataccctca gattatagca tcccaggctt tcaggttccc
     6481 ggttccctca gcctctgccc gccgcgcctg gaaacttgct ataataacgc gtaacgcaat
     6541 gaaaaaagga agtaattatg acgcctgaca gcatcacgcc ggaaatgttt gccaaa
//
LOCUS       DAOQAD010000099         6583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_14284_length_6583_cov_5.138327, whole genome shotgun sequence.
ACCESSION   DAOQAD010000099 DAOQAD010000000
VERSION     DAOQAD010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14284_length_6583_cov_5.138327"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKL60_07360"
     CDS             <1..360
                     /locus_tag="PKL60_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW95988.1"
                     /translation="DSSAHYTDGRRGLTGKALILPPVPGKKTPSEFERQWQLAASLLV
                     KAERIVVFGFAFNPYDVEILELFRNYSAEVKSVLLIDIKPNVDTCRYIWPYASITTLQ
                     PPPESERDIREWLSKVA"
     assembly_gap    589..688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            963..1643
                     /locus_tag="PKL60_07365"
     CDS             963..1643
                     /locus_tag="PKL60_07365"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNW95989.1"
                     /translation="MSSENWSELLFQALESAPNPEKAPAMEAYMKHNFRYLGLPSPRL
                     MELAGPHLKAASQQPGIDWDFVQAAWQKPYREAQYCACAYLSRVRARLTAEDLPALKR
                     LVQTKSWWDSVDTLSGIIGSLVLRCPALKQDILAWSLDQDLWTRRAAIIHQLEYKKDT
                     DTALLEQILLNNLGTREFFINKAVGWSLRQYSRTDPAWVAAFVRAHEPELANLSKKEA
                     RKYLPPET"
     gene            complement(1727..2437)
                     /locus_tag="PKL60_07370"
     CDS             complement(1727..2437)
                     /locus_tag="PKL60_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNW95990.1"
                     /translation="MSDLLSEDQPACLERVCAQVIQCRACPRLVSWREQVAAQRRRAF
                     LEQTYWGKPVPGLGDPRARLLAVGLAPGAHGSNRTGRMFTGDASGDFLFPALFRAGFA
                     SSPRSEGVGDGLRLRDLYITAVCHCAPPGNKPAPEEIRACQTWLQAELNCLPGLKGIV
                     LLGKVALEGVLALPMYAGVRRKEVPFGHGAFYAPEGLPWLLCSYHPSRQNTQTGKLTP
                     EMFDSIWQQAGRLLSGEA"
     gene            complement(2613..3902)
                     /gene="eno"
                     /locus_tag="PKL60_07375"
     CDS             complement(2613..3902)
                     /gene="eno"
                     /locus_tag="PKL60_07375"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNW95991.1"
                     /translation="MDTTIEYVNAMEVLDSRGNPTVEVEVILGDGSFGRAIVPSGAST
                     GVHEALELRDGDKSRYGGKGVLDAVDHVNDEIADELIGWDAIEQKAIDQLLLEMDGTP
                     NKSALGANAILGTSLAVAKAAAGSLGLPLYRYLGGVYAHVLPVPMMNILNGGAHTGWQ
                     STDVQEFMAMPLGAETFAEGLRWGAEIYQSLKGVLKSKGYITLVGDEGGFAPQLKANE
                     EAVELILEAITKAGYKPGEDVCIALDPAASELYDEDTRLYNLRKEGRKLTSDQMVEFW
                     ADWVRKYPIVSLEDGFAQDDWDGWKNFTARMGQRLQIVGDDLLVTNPERVARAIEEKA
                     CNALLVKLNQIGSLTETMQAVSMVQTHGWKAVCSHRSGETEDSTIADLAVAMNMGQIK
                     TGAPARSDRVAKYNQLLRIENELEDTARYAGWSALAR"
     gene            complement(3969..5606)
                     /locus_tag="PKL60_07380"
     CDS             complement(3969..5606)
                     /locus_tag="PKL60_07380"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNW95992.1"
                     /translation="MTTKVIFFTGGVLSSVGKGVTAAAIGRLLKARGFNITIQKLDPY
                     LNVDPGTLSPYQHGEVFVLDDGAETDLDLGHYERFCDLRLTRNSTVTTGQVYQEVLDR
                     ERRGDYLGGTIQVIPHITNEIKRRIALVGKASNADIALVEVGGTVGDIEGQPFLEAIR
                     EMRNDLGRGNTAFVHLTWLPYLKATEETKTKPTQHSVRELRSIGILPDMIVARSDYPV
                     ERTLCAKIAQFCDVPVEAVIPLETADLLYRIPLVLEEMRVAEILLNRLNLTPKQEPDL
                     DKWRWMVAELGRKKPAVRIGLVGKYVELHDAYLSVREAIKHAALYAGVDVEMDWISAT
                     DLEKGIGLERLENVDGILVPGGFGSRGVEGKILAAKYAREHKLPYLGLCMGMQVMVIE
                     YGRSVLGFSDANSSEFDKLSAHPVIDLMPEQTNLEETGGTMRLGLYPCDIQPGTLAAR
                     LYQAEHVNERHRHRYEVNNDFRALYEQNGMVFSGLSPNRVLVEIAELPGHPFMIGCQF
                     HPEFLSRPTAPHPLFAGFIAAVCQRCGLPVEEHFPNS"
     gene            complement(5719..6504)
                     /locus_tag="PKL60_07385"
     CDS             complement(5719..6504)
                     /locus_tag="PKL60_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNW95993.1"
                     /translation="MESPIEQASSTQSSLKPKQKFEGKVMKTSLQGAILDIGEKVPGF
                     LHISQVINTAKPDAVVKNLEDVIKVGDTVSVWVRRILKDHVELTMHEPLGLEWREIQP
                     DMVLHGKIVRLESFGVFVELGAERPGLVHVSEISHNYVRVPSEVVKIGDEVDVKVLEV
                     DKRKKQIKLSMKALQEAPEPEETEQKRPARKGKARQEVEVEVPAEEVIPDPTFMEIAL
                     RQAMDRAEKRKPQISNRVKRAKDLDSEQDDIFDRTLKGKQNKD"
BASE COUNT         1345 a         1989 c         1732 g         1417 t
ORIGIN      
        1 gactcaagcg ctcactacac ggatggccga cgaggcttga ctggaaaggc cctgatcctc
       61 ccgcctgttc ccgggaagaa aacgccgtcg gaattcgaaa gacaatggca gttggccgca
      121 agccttctcg tgaaagcaga gcgaatagtg gtgtttggat ttgccttcaa tccgtacgat
      181 gttgagatac tcgaactctt cagaaactac agcgcagaag tcaagagcgt tctgcttatc
      241 gatattaagc ctaacgttga cacctgcagg tacatctggc catatgcatc catcactact
      301 ctacagccac cgcctgaatc cgaacgcgac attcgcgagt ggcttagtaa agtagcgtga
      361 ccttgacctc gccatctacc acgcgcgtcc agcggacttg actttcgggc tcgccactga
      421 ctcgccagct gttagcaaac cttaatgagt caaaaccttc agtttcgcca ctccaatttg
      481 agttaccagt accttaggaa ccactgaagg aaaaataacg gactttaatc accccggtct
      541 cagaccacgc gcgaaattct gccgagagat atccgatatc cggcatccnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tttcaggaga cctacggtca agcccttcgc
      721 tcggtcatga gaccttcgcg aacgtcatcc ccgatcacgc cggtctcaga ccgcgcgcga
      781 aattctgccg agaactatcc gatatccggc atcccctttc aggagaccta cggtcaagcc
      841 cttcgctcgg tcatgagacc ttcgcgaaca tcagtttctt attctcatga acagccagag
      901 gacctcaacg cgtataattt agatcagaaa accctcaatc cccacacgga aaagagaacg
      961 ccatgagcag cgaaaactgg agcgagttat tatttcaagc ccttgaatcc gcgccaaacc
     1021 ccgaaaaagc cccggccatg gaagcctaca tgaagcacaa tttccgttat ctcggtctgc
     1081 cctcgccgag gttgatggag ctggccggtc cgcacctcaa agccgccagc caacaacccg
     1141 gaattgactg ggatttcgtt caagctgcct ggcaaaaacc ctaccgcgaa gcgcaatatt
     1201 gcgcctgcgc ttatctcagc cgcgtccgcg cgcgattgac tgcggaagac ctgccggccc
     1261 tgaagcgcct ggtgcagacc aaatcctggt gggacagcgt ggacactttg agcgggatta
     1321 ttggcagcct ggtgctgcgc tgcccggcgt tgaagcagga catacttgcc tggagcctgg
     1381 accaagattt atggacccgg cgcgcggcca tcattcacca attagagtac aaaaaagata
     1441 cggataccgc cctgctggag caaatcctcc tgaacaacct cggcacgcgc gaattcttca
     1501 tcaacaaagc cgtcggctgg agcctgcgcc agtacagccg cacggaccca gcgtgggtgg
     1561 cggcctttgt ccgcgcgcac gaacccgagc tggccaacct gagcaaaaag gaagcgcgga
     1621 aatatctgcc gccggaaact taatcccccc gtcgtttttt tccgactccc cacgcctgtt
     1681 caaagaaggc gtcccgacct gaaggcaacg cgcatcccca actgcttcag gcttctcccg
     1741 aaagcagccg cccggcttgc tgccagatcg agtcgaacat ttcgggtgtc agcttgccgg
     1801 tttgggtgtt ttggcggctg ggatggtacg agcacagcag ccagggcagc ccctccggag
     1861 cgtaaaaagc gccgtgccca aacgggactt ctttccgccg cacacccgcg tacatcggca
     1921 gcgccaatac gccttccaat gcgactttcc cgagtaaaac tatccccttt aatccgggca
     1981 ggcagttcag ttcagcctgc agccaggttt gacaggcgcg gatttcctca ggcgcgggtt
     2041 tattaccggg cggcgcgcag tggcagaccg ccgtgatata caggtcccgc aggcgcaagc
     2101 cgtccccgac cccctcagag cgggggctgc tggcaaaacc agcccgaaac agcgccggga
     2161 acaggaagtc cccggaagcg tcgcccgtga acattctgcc ggtgcggttg gagccgtgcg
     2221 cccccggcgc cagcccgacc gcgagcaaac gcgcgcgcgg gtcccccaag ccgggtacgg
     2281 gtttccccca ataggtctgc tccaaaaacg cgcgccggcg ctgcgcggcc acttgctcgc
     2341 gccaggagac caggcggggg caggcccggc attggatgac ctgcgcgcaa acccgttcca
     2401 aacaggccgg ctgatcctct gaaagcaaat cgctcatgcg cacgattata accggccgaa
     2461 tcgctaaaca gcttgccctg gcaactgttt cgacctgaaa ggattattca agattgccgg
     2521 gcaatggtca ttagggtttc gttcttgttc agcccttgca tgaaaaaacg gccgccccca
     2581 gtggacggcc gttcattcgg caaatgcgct gtttagcgcg cgagagcgga ccaacccgcg
     2641 tagcgggcgg tatcttccag ctcattctcg atgcgcagca gctggttgta cttggccacg
     2701 cggtcggagc gtgcgggcgc gccggtctta atctgaccca tgttcatggc gacagccagg
     2761 tcagcaatcg tggagtcttc ggtctctccg gaacggtgcg aacaaactgc tttccagccg
     2821 tgggtctgca ccatgctgac cgcctgcata gtttcggtca gggagccaat ctggttcagc
     2881 ttgacgagca gcgcgttgca ggctttttct tcaatcgcac gggcgacgcg ttcggggttg
     2941 gtcaccagca ggtcgtcccc cacaatttgc aggcgctgcc ccatcctggc ggtaaagttc
     3001 ttccaaccgt cccagtcatc ctgcgcgaag ccgtcttcca gggagacaat gggatacttt
     3061 ctcacccaat cagcccagaa ttccaccatc tggtcgctgg tcagcttgcg gccttccttg
     3121 cgcaggttgt aaagtctggt gtcttcatca taaagttctg aagcagccgg gtcgagcgcg
     3181 atgcagacat cctcgccggg tttgtagccg gctttggtga tggcctccaa aatcagctca
     3241 acggcttcct cgttggcttt cagctgcggc gcgaagccgc cctcatcgcc caccagggtg
     3301 atataaccct tggatttgag cacgcctttc agggactggt agatttccgc gccccagcgc
     3361 agcccttcgg cgaaggtttc agcgcctagc ggcatagcca tgaattcctg gacgtcggtg
     3421 gactgccagc cggtatgcgc gccgccgttc aggatgttca tcatcgggac gggcagcaca
     3481 tgcgcgtaca cccctcccag atagcggtac aacggcagcc cgagcgagcc ggctgcggct
     3541 ttggcgactg ccaggctggt gccgaggata gcgttcgcgc ccaaagcgga tttgttcggc
     3601 gtgccgtcca tttccagcag cagctggtca atcgcctttt gctcaatcgc gtcccaacca
     3661 atcagttcgt ccgcgatttc atcattcacg tggtcgacag cgtccaatac gcctttgccg
     3721 ccgtagcggg atttatcgcc atcgcgcaat tccaaagctt cgtgcacgcc ggtggaagcc
     3781 ccggaaggca caatcgcccg tccgaagctg ccgtctccca ggattacttc cacttctact
     3841 gtcgggttgc cgcgggagtc caaaacttcc atcgcgttta catattcgat cgttgtgtcc
     3901 ataggtctga tcctctctgg tatccaaaat gaattgccta agtatactcc aatagtttga
     3961 gaaaattttc acgagttagg gaaatgctcc tcaacgggca gcccacaccg ctggcatacc
     4021 gcggcgatga acccggcgaa tagcgggtgc ggcgcggtcg ggcgtgagag gaattccggg
     4081 tggaactggc agccaatcat gaacggatgc cctggcagct ccgcgatttc caccagcact
     4141 cggttcgggg aaagccccga gaacaccatc ccgttttgct cgtacaaagc ccggaaatca
     4201 ttgtttacct catagcgatg gcggtggcgc tcgttcacgt gttcagcctg gtacaagcgg
     4261 gcagccaatg tccccggctg gatatcgcag gggtacaaac caagccgcat cgtgccgccg
     4321 gtctcttcca gattggtttg ttcgggcatc aggtcaatca ccgggtgcgc gctgagcttg
     4381 tcaaactcgg aggagttcgc gtcgctgaat cccaacacgg agcgcccata ctcaatcacc
     4441 atcacttgca tccccatgca caagcccagg taaggcagct tatgctcgcg ggcatatttc
     4501 gccgccagga ttttcccttc caccccgcgc gaaccaaaac cgcccggcac gagaatgccg
     4561 tccacgttct ccaggcgctc caacccgatg cctttttcca ggtcggtggc ggaaatccaa
     4621 tccatctcca catccacgcc ggcatagagc gcagcgtgct tgatggcttc ccgtacgctc
     4681 aggtaagcgt cgtgcaattc cacgtatttc ccgaccaggc caatcctgac cgcgggcttt
     4741 tttcttccca gctcagccac catccagcgc cacttgtcca ggtcaggctc ttgcttgggt
     4801 gtcaggttca gccgattcag caggatttcc gcgacccgca tctcctcaag cacgagcgga
     4861 atgcgataaa gcaagtcagc ggtctccaga gggatgaccg cctcgacagg cacatcgcag
     4921 aactgcgcga tcttggcgca caaagtccgc tccaccgggt aatcgctgcg cgcaacaatc
     4981 atatccggca ggatgccgat ggaacgcagt tcgcgcaccg aatgttgggt cggtttggtc
     5041 ttggtttctt ccgtcgcctt caggtagggc agccaggtca ggtgcacgaa ggcagtatta
     5101 ccgcgcccca ggtcgttgcg catttcacgg atggcctcca gaaagggctg accctcgata
     5161 tccccaaccg tgccgccaac ttccacaagc gcgatatccg cgttggacgc tttgccgacc
     5221 agggcgatgc gccgtttgat ttcattggta atatgcggga taacctgaat cgtaccgccc
     5281 agatagtccc cgcgccgctc ccggtccaga acctcctgat agacctgtcc ggtggtaacc
     5341 gtgctgttgc gggtcaggcg caggtcgcaa aagcgttcat agtgccccag gtccaggtca
     5401 gtctccgcgc cgtcatccaa cacaaagacc tctccgtgct gataagggct gagcgttccg
     5461 gggtccacgt tcaggtaggg gtccagtttt tgaatggtaa tgttgaatcc gcgcgctttc
     5521 aacagccgtc cgattgccgc cgcggtaaca cccttgccaa ccgatgagag cacaccccct
     5581 gtgaagaaga taactttggt ggtcatccgg cccctccaat catgctttat cgcatttaaa
     5641 attttcgggg agggttgttt tgcggaccct ccccggttca ttcaatctaa cttaggctgt
     5701 gtaatggcaa gcagcatgct aatccttatt ttgcttccct ttaagcgtgc ggtcaaaaat
     5761 atcatcctgt tcagaatcca gatctttggc gcgtttgacg cggttggata tctggggctt
     5821 gcgtttttcc gcgcggtcca tcgcctgccg taacgcgatt tccatgaagg tcggatccgg
     5881 aataacttct tcggcgggca cttcgacttc gacttcctgc ctggctttgc ccttcctggc
     5941 ggggcgtttc tgttccgtct cttcgggctc gggggcttcc tgcagagctt tcatgctcag
     6001 tttgatttgc ttcttgcgct tgtcgacttc cagaaccttc acgtccactt cgtcgccaat
     6061 cttgacaact tcggagggga cgcgcacata attgtgcgaa atctcgctga cgtgcaccag
     6121 acccgggcgc tcggcgccga gttctacgaa gacgccaaaa ctttccaaac gcacgatctt
     6181 tccgtgcagc accatgtcgg gttgaatttc gcgccattcc aggccgagcg gttcgtgcat
     6241 ggtcagttcg acatggtctt tgaggatacg gcgcacccag acgctgactg tatcgcctac
     6301 cttgataacg tcttcaaggt ttttgacgac cgcgtccggc tttgcggtgt tgatgacctg
     6361 agaaatatgc aggaagcccg gcaccttttc accgatatca aggatcgcgc cttgcagcga
     6421 ggttttcatc acctttcctt cgaatttttg tttcggtttc aggctggatt gtgtagagct
     6481 tgcttgttcg atgggacttt ccatagtatt tctccgggag aattagttca cgattggcaa
     6541 gtatactcta cgagataaac ctttgtcaaa tctcaattgt ggg
//
LOCUS       DAOQAD010000100         6558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_14357_length_6558_cov_6.452407, whole genome shotgun sequence.
ACCESSION   DAOQAD010000100 DAOQAD010000000
VERSION     DAOQAD010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14357_length_6558_cov_6.452407"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PKL60_07390"
     CDS             <1..1041
                     /locus_tag="PKL60_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW95994.1"
                     /translation="GAPIAALAFGLVPLANSLPLFVACTVTTILAMAFWRTPVVALMP
                     DVTPSRLRSQANGIINFMGGLGLVLGTSIGAMLVKQNQAYPFWFGSLLVLGAAALVLI
                     FVREPKVYENNPNVEKPNMWKSLKELFTSKHGDAIRIFLAIFFWFIAYNAIEAFLSLY
                     GINHLGLDESGAGKLITSVGFSFLLFSIPAGFIGAKLGRRKTISIGLLIMMLVMLAVY
                     FLPKEMLLQTHGQLPILGDLMNLTILLLVAGIGWACININSLPVVVDLTDPIHIGTYT
                     GLYYLFSTLAAIAGPNINGWIVKLTGNNYASIFIIGPIFMAAAFAMVFGMHDGEAKAR
                     PEPAGSAAPEQN"
     gene            1132..2172
                     /locus_tag="PKL60_07395"
     CDS             1132..2172
                     /locus_tag="PKL60_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNW95995.1"
                     /translation="MSKRVITLLSLLIVAGMLLAACGTTATEAPAAKIKVCQITDTGG
                     IDDKSFNATAWKGIEDAVAQLGIEGKYLESKEVADYEKNLNAFVEEKCDLIITVGFLI
                     GDATKATAEANPDIKFSIVDYAYDPAIPNVVGQVFNTDEAAFLAGYLAAGVTKTGKVG
                     TFGGLPIPTVTIFMDGFARGVAKYNEVKGTNVEVLGWDPANPDSGLFSMSFDDQQKGR
                     ELAVSLMDEGADIIMPVAGPVGLGAAAAIKERGNAYLIGVDSDWFLTSPEYADITLSS
                     VMKLMDATTMQVIKSVIDGTFQGGVVVGTLANNGVALAPLHNLESLASAELLAEIEQL
                     KADIIAGTLSVK"
     gene            2278..3837
                     /locus_tag="PKL60_07400"
     CDS             2278..3837
                     /locus_tag="PKL60_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW95996.1"
                     /translation="MAPILELRGITKQFPGVLANDHIDLALNKGEILALLGENGAGKS
                     TLMNILYGLYQPDEGEILINGEPVKITSPTDAINQGIGMVHQHFMLIPVFTVTENVML
                     GDESVKTGGFLDRPKAAQRIREISSSYKLEVNPGDYVRDLPVGVQQRVEIIKLLYRNA
                     EILIFDEPTAVLTPQEADELFNIMRSLVAQGKSIIFITHKLREVLAISDRVLVIRRGK
                     VVGETTPEVADKNSLAAMMVGREVNLVVKKKETIPGDVVCSVQDLVVADKFMNVVVDD
                     VSLDIRAGEILGIAGVQGNGQTELVEAITGLTKLASGRITLLGQDISKAAPRRITELG
                     SAHVPEDRQADGLVLPFSVAENLVLCTYHLQPFSRGIILQYDTILKNAEKLIQDFDIR
                     TPSPLTPVGSLSGGNQQKVIIARELSRPIQFLVASQPTRGLDVGSIEYIHKRIEQKRE
                     EGCAVLLVSPELDEIMELSDRIAVMYRGKILAVVQNKGVSKEYIGLLMAGVVPEEPLQ
                     VSEETEIEATL"
     gene            3849..5123
                     /locus_tag="PKL60_07405"
     CDS             3849..5123
                     /locus_tag="PKL60_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95997.1"
                     /translation="MSEAQAEKKDLGQALLKEINPPPEEEHKKGFWKSISIPALAILT
                     GLLIGAFMIAATSTTVWAAFGESIWRGFGTAFKEIGTAYQALFTGALGDPVRIVRALG
                     SGDAKEIRSALNPFLESLVQSTPYIFTGLAVAVGFRSGLFNIGVEGQLFIGAAAATYV
                     GYAVTGLPGFIHMPLALLAGALGGAFWAFIPGFLKAKTGGHEVINTIMMNWIAFRLTE
                     WLLSGPMSRPGSGGMPLSPLIQKSAEIPQFFQAPIRFHLGFFIALAVAWLINWLLFKT
                     TWGLNLRTVGTNPRAAKYAGLSTTKSIITAMLISGGLAGIAGAVQILAVNHSMALGLS
                     SGYGFDSIALALIGNNTPLGVILASLLFGTLRNGATRMMVVSAIPIDIVDVIQAIILM
                     FVAAPAIIRAIYHLKQPKKEETTIFVSGWGGK"
     gene            5120..>6171
                     /locus_tag="PKL60_07410"
     CDS             5120..>6171
                     /locus_tag="PKL60_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95998.1"
                     /translation="MMTTITQTIKHRRISEITPARKVSMGIVEILIGILIYILFAAAL
                     APELQTTFNMTPGGINVGTMADWVLPSRATLLVFTLICIGIGAFQLIKGFGRATNGMV
                     GLCGLLFVMAFLIWQASGKSLNLSGMLSSAVLLAVPITLAAFSGILAERSGTINIAIE
                     GMMLMSSMFAALIGSLTNSAVLGLLGGVLSSVALAMLHAVLCIKYKINQTVSGTVINI
                     FSAGMTAFISQKFLQKYQNLNAPPMLSRFPVPLLSRIPFIGPIVFNQNLFVYLMLVIL
                     VVLQLALFSTRWGLRLRSVGEHPKAADTLGIDVIRTRYVGMLLSGVVAGIAGAFFTLG
                     SVGRFDEGMTAGKGFIG"
     assembly_gap    6172..6181
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6182..6363
                     /locus_tag="PKL60_07415"
     CDS             <6182..6363
                     /locus_tag="PKL60_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW95999.1"
                     /translation="AGLLFGFADAIGSKLSLLGSAIPPQVMGMAPYLITMIVLAGFIG
                     KGQAPAAEGKPYEKE"
BASE COUNT         1342 a         1957 c         1813 g         1436 t
ORIGIN      
        1 ggcgcgccga ttgccgcgct ggcttttggt ctggtcccgc tggccaattc cctgccttta
       61 ttcgtagcct gcaccgtcac gaccattctg gcgatggcct tctggcgcac gccggtggtc
      121 gcactgatgc cggacgtcac gccttccagg ctgcgctccc aggccaacgg gatcattaac
      181 ttcatgggcg ggctggggct ggtgctgggc acttccattg gcgcgatgct cgtcaagcag
      241 aaccaggcct atcccttctg gttcggctcg ctgctggttt taggcgcggc cgcgctggtg
      301 ctcatcttcg tgcgcgagcc caaggtgtac gaaaacaacc ccaacgtgga aaagccgaat
      361 atgtggaaga gcctcaagga gcttttcacc agcaagcatg gggacgcgat tcgcatcttc
      421 ctggccatct ttttttggtt catcgcctac aacgccatcg aagccttcct ttcgctctac
      481 ggcatcaacc atctcgggct ggatgaatcc ggcgcgggca aattgattac cagcgtgggc
      541 ttctcgttcc tgctcttttc catccctgca ggcttcatcg gcgcgaaact cggacgccgc
      601 aagaccatca gcatcggtct tctcattatg atgctggtta tgctggctgt gtatttcctc
      661 cccaaggaaa tgctccttca gacccacggc cagctgccta tcctgggtga cttaatgaac
      721 ctgaccatct tgctgctggt ggcaggcatt ggctgggcct gcatcaacat caactcgctg
      781 cctgtggttg tggacctgac cgacccgatt cacattggca cctacaccgg cttgtattac
      841 ctcttttcca cgctcgccgc gattgcaggt ccgaacatca acggctggat tgtcaaactg
      901 accggcaaca attacgcctc catttttatc atcggaccca tttttatggc ggccgcgttc
      961 gccatggtat tcgggatgca cgatggtgag gcaaaggccc ggcccgagcc tgccggcagc
     1021 gccgcgcctg agcaaaactg atatcttgca ttgtattcct gaggcaagta tacttaagac
     1081 agtgcaaacc ggttttccgg aaactaaatg tgtattgagg agaaacacac aatgagtaaa
     1141 agagttatca ccctgctgtc ccttttaatt gtggctggca tgcttttggc tgcttgcggc
     1201 accaccgcga ccgaagcccc cgccgcgaaa atcaaagtct gccagattac tgatactggc
     1261 ggcattgatg acaaatcctt caacgccact gcctggaaag gcatcgaaga tgctgtcgcc
     1321 caacttggca tcgaaggcaa atacctcgag tccaaagaag tcgctgacta cgaaaagaac
     1381 ctgaacgcgt tcgtcgaaga aaaatgcgac ctgatcatca ccgtcggttt cttaatcggc
     1441 gatgcgacca aagccaccgc tgaagccaac cccgacatca agttctccat cgttgactat
     1501 gcttacgacc ccgctatccc caatgtcgtc ggtcaggtgt tcaacaccga cgaagccgcc
     1561 ttcctggctg gctatctcgc cgctggcgta accaagaccg gtaaggtcgg taccttcggc
     1621 ggtctgccca tccccaccgt caccatcttc atggatggtt tcgccagggg cgttgccaag
     1681 tacaacgaag tcaagggtac gaacgtggaa gtcctcggct gggatcccgc caatccggac
     1741 tccggcctct tcagcatgtc cttcgatgac cagcagaaag gccgcgaact cgccgtctct
     1801 ctgatggacg aaggcgctga tatcatcatg cccgtcgccg gaccggttgg tttgggcgca
     1861 gctgccgcga tcaaagaacg cgggaacgcc tacctcatcg gtgtcgactc tgactggttc
     1921 ctgaccagcc ccgaatacgc cgacatcacc ctgagctccg tcatgaaact gatggatgcc
     1981 accaccatgc aggtcatcaa atccgtcatc gacggcacct tccagggcgg cgtcgtggtc
     2041 ggtaccctgg cgaataatgg tgttgcgctc gccccgctgc acaacctcga gagcctcgct
     2101 tccgcggaac tcctggctga aatcgagcag ctcaaggccg acatcatcgc tggaaccctt
     2161 tccgttaagt aaagcacccc acagcagtac ccggcgggct ggcaaattgc cagcccgctt
     2221 tttctatccc ctgtggtaag attaagcatt aaatcttcct ttgaaaaggg gtttcctatg
     2281 gctccaattc ttgaactcag gggtatcacc aaacaattcc cgggcgttct cgcgaatgac
     2341 catatcgatt tagcgctgaa caaaggggaa atactggcgc tgctgggcga aaacggcgcg
     2401 gggaaatcca ccctcatgaa catcctgtat gggctctatc agcccgatga aggcgaaatc
     2461 ctgattaacg gcgaacccgt caagattacc tcaccaaccg acgccatcaa tcagggcatc
     2521 ggcatggtcc accagcattt catgctgatt ccggtcttca ccgtgaccga gaacgtgatg
     2581 cttggggatg aatccgtgaa aaccggcggc tttttggacc gccccaaagc tgcccagcgc
     2641 atccgggaga tttccagcag ttacaagctg gaagtgaacc cgggcgatta cgtacgcgac
     2701 ctgccggtcg gcgttcagca gcgcgtggaa attatcaagc tgctctaccg caacgcggaa
     2761 atcctgattt ttgacgagcc gaccgccgtc ctgaccccac aggaagcgga tgaacttttc
     2821 aacatcatgc gctcactggt ggcgcaagga aagtccatca tcttcatcac gcacaaattg
     2881 cgcgaagtgc tggcaatctc cgaccgcgtg ctggtcatcc ggcgcggaaa agtggtgggc
     2941 gaaaccaccc cggaagtcgc ggacaagaac tcgctggccg ccatgatggt cgggcgggaa
     3001 gtcaacctgg ttgtcaaaaa gaaagaaact atccctggtg atgtggtttg ttctgttcag
     3061 gacctggtcg tggcggataa gttcatgaac gttgtcgtgg acgacgtctc cctggacatc
     3121 cgcgcgggag aaatccttgg gattgccggc gtccagggca acggccagac cgagctggtg
     3181 gaagcaatca cgggcctgac caaacttgcc tccggcagga tcacgctgct cgggcaggat
     3241 atatccaagg cggctccccg ccggatcacc gagctcggtt ccgcccacgt cccggaggac
     3301 cgccaggcgg acgggttggt gctgcccttc tccgtggcgg aaaacctggt cctgtgcaca
     3361 taccatctcc agcctttttc caggggcatt atcctgcagt acgatacgat tctcaagaat
     3421 gccgaaaaac tgattcagga ttttgatatc cgcaccccca gcccgctcac tcccgtgggc
     3481 tcgctctccg gcggcaacca acagaaagtg atcatcgcgc gcgagctttc ccgaccgatt
     3541 cagttcctgg tcgcctccca gccaacccgc ggtctggacg tcggctccat tgaatatatc
     3601 cacaaacgta tcgagcagaa acgcgaggaa ggctgcgcgg tcttgttggt ctcgcctgag
     3661 ttagacgaaa tcatggagct ctcggaccgc attgccgtga tgtaccgcgg caagattctg
     3721 gcggtggtcc aaaacaaagg cgtcagcaag gaatatatcg gcctgctgat ggccggggtg
     3781 gtacctgaag agccgctgca ggtttccgaa gaaactgaaa tcgaagcaac cctttaagag
     3841 gtggtcgcat gagtgaagca caagcagaaa agaaagattt aggtcaggct ctcctgaaag
     3901 aaatcaatcc gccgcccgag gaagaacaca agaaaggctt ctggaagagc atttccattc
     3961 ccgcgctggc tatcctgacg ggcttgctta tcggcgcgtt catgattgca gccaccagca
     4021 caaccgtgtg ggcggctttc ggtgaatcca tttggagagg ttttggcaca gcattcaagg
     4081 aaattgggac agcttatcag gcgctcttca ccggcgcgct cggcgacccg gtgcgcatcg
     4141 tacgggcttt aggctccggc gacgcaaagg aaatccgctc cgcgctgaac ccattcctgg
     4201 aaagcctggt gcagtccacg ccgtacatct tcaccggtct ggcggtagcg gtcgggttcc
     4261 gcagcggttt gttcaacatc ggcgtggaag ggcagctctt catcggcgcc gccgcggcca
     4321 cctacgtggg ttatgccgtc accggcttgc ccggcttcat ccacatgccg ctggcgctgc
     4381 tggccggcgc actcggaggc gctttttggg cgttcatccc cggcttcctg aaagcgaaaa
     4441 cgggcgggca tgaggtgatt aacacgatta tgatgaactg gatagccttc cgcctgacgg
     4501 aatggctgct atccggtcca atgtcccggc cgggctcggg cggtatgccc ctcagcccgc
     4561 tcatccaaaa gtctgccgaa attccgcagt tcttccaggc gccaattcgt ttccacctgg
     4621 gtttcttcat cgcgctggca gtcgcctggt tgattaactg gctgctcttc aaaaccacct
     4681 ggggcttgaa tctgcgcaca gtcggcacga acccgcgcgc ggcaaaatac gccgggttga
     4741 gcaccaccaa atccatcatc accgcgatgc tcatttccgg cggattggcc ggtatcgccg
     4801 gcgcggtgca aattctggcg gtcaaccaca gcatggcgct cggcctttcc tccggctacg
     4861 gcttcgacag catcgcgctg gcgttgattg ggaacaatac cccgctgggc gtcatcctgg
     4921 cctcgctgct ctttgggacc ctgcgcaacg gcgccacgcg catgatggtg gtttccgcca
     4981 tcccgattga catcgtcgac gtcatccagg ccattatcct gatgtttgtc gcggctcccg
     5041 caattatccg ggcgatttat catctcaagc agccaaaaaa ggaagagact accatcttcg
     5101 tcagcggttg gggaggtaaa tgatgaccac tataacgcaa acgattaagc accgacgtat
     5161 ttcggaaatc acacccgctc gcaaagtgag catggggatc gttgagattc tcatcggcat
     5221 tctgatttac atcctgtttg ccgccgcgct tgcgccggaa ttacagacca cctttaatat
     5281 gactcccggc ggaattaacg ttggcacgat ggccgattgg gtcttgccct cgcgcgctac
     5341 gctgctggta ttcacgctga tttgcatcgg gattggcgct ttccagctca tcaagggttt
     5401 cggacgcgcc accaacggca tggtcggctt gtgcgggctg ctctttgtga tggcattcct
     5461 gatttggcag gccagcggaa aatccctgaa cctctcgggc atgctttcca gcgcggtgct
     5521 gttggctgtg ccgattacat tggcagcttt ctccggcatc ctggcggaac gctccggaac
     5581 catcaacatc gccatcgaag ggatgatgct gatgtcctcc atgttcgccg ccctgattgg
     5641 cagcctcacc aacagcgcgg tcctcggtct gctgggcggc gtgctctcct cggtcgcgct
     5701 ggccatgctg catgcggtgc tgtgcatcaa atacaagatc aaccagacgg tatccggcac
     5761 ggtcatcaac attttctcgg ctggcatgac cgcctttatc tcgcagaaat tcctgcagaa
     5821 ataccagaac ctgaacgccc cgccaatgct ctcgcgcttt ccggtgccgc tgctctcgcg
     5881 catcccattc atcgggccga ttgtattcaa ccagaacctt tttgtgtacc tgatgctcgt
     5941 gattttggtg gtgctgcagc ttgcgctctt ctccacccgc tggggactca ggctgcgctc
     6001 ggtaggagag caccccaagg ccgcggatac gctcggcatc gatgtcattc gcacgcgcta
     6061 cgtcggcatg ctgctgagcg gcgtggtggc gggcattgcc ggagcgttct ttacgcttgg
     6121 ctccgtcggg cgctttgatg agggtatgac cgccgggaaa ggcttcattg gnnnnnnnnn
     6181 ngggccggcc tgctctttgg cttcgcagac gcgattggct ccaaactctc cctgctcgga
     6241 agcgccattc cgccccaggt catgggcatg gcgccttacc tgattactat gattgtgctg
     6301 gcaggcttta ttgggaaagg acaggcaccc gctgcggagg gcaagccgta cgaaaaagag
     6361 tagcgtgttc catcacagac gcccccgcct ttgtgcgggg gtgttttgtt ctggtagaac
     6421 atttgcatga cctgtcagtg agcggcagcg caacccaaca gaacgtggcc gtaggtcggg
     6481 tggagccgta ggtcgggtgg agcgtcagcg caacccgaca aaacatgctc gtaggtcggg
     6541 tggagccgta ggtcgggt
//
LOCUS       DAOQAD010000101         6470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_14645_length_6470_cov_6.250507, whole genome shotgun sequence.
ACCESSION   DAOQAD010000101 DAOQAD010000000
VERSION     DAOQAD010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14645_length_6470_cov_6.250507"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..1030)
                     /locus_tag="PKL60_07420"
     CDS             complement(209..1030)
                     /locus_tag="PKL60_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNW96000.1"
                     /translation="MPEILANKVRLYYELHGPEDGEVLVLSNGIMMSTGSWFNQTRAL
                     AAHMRVLLYDCRGMWRSEHPAGPYSLEQHADDLAALLDGLGIDQAHIGGISYGSEISM
                     VFALKYPEKTRSLIVIDGVSEIKPMLAAQTYPWQLAAEHNDPEMLYWTSVHLNFSAEY
                     VAKTRRSAGEMIKNYGALDKVSFVELMKAFYALDITNELPKIKCPTLVVVGGDDLIKG
                     REYAQIIVDQIQGAEYVVVPGSGHALCLDKPDPLNTLLIGFVLKNQKPKDKQGGS"
     gene            complement(1045..1464)
                     /locus_tag="PKL60_07425"
     CDS             complement(1045..1464)
                     /locus_tag="PKL60_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HNW96001.1"
                     /translation="MSDCKLETKTFYQAIENEVLIGSRCLNCGHISIPQRYICSRCHS
                     TQAERIEFIGTGKLAAFTVIYVPATEMVKAGYDSKNPYMVGIVTLDEGPRISAQILGM
                     DLTDPAKIKIGTPLKMTVVERGPEGQAKKFLAFEPIQ"
     gene            complement(1466..2731)
                     /locus_tag="PKL60_07430"
     CDS             complement(1466..2731)
                     /locus_tag="PKL60_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNW96002.1"
                     /translation="MTGKKLARGVAVAGVGMSKFGVFPDKSSRDLFVEAYQELRSSVE
                     KGLPPKDIEAFYLGNFSSDQFEKQGHLAPIVASWIGISPVASARMEDACASGGAALRQ
                     AILAIASGQYDVVLVGGLEKMTDLPTAAVTDALASAADTMFEIPAGFTFPGFYAAMAT
                     AYMDKYQADPDAFLCVGIKNHENGALNEKAQFNQTIKDVMAGKKAKAEQKGLPVPTWE
                     NEMDFMHDPKANPMIAWPLRLFDCSPVSDGAAAVLLVSEELASKYTDHPITIAGIGQA
                     SDGALAERDSLSEVPSASKAAAQAYEMAGISAADIDFAEVHDCFTIAEIMASEDLGFF
                     PRGEGWKAAMEGKTSRQGEHPINTSGGLKSKGHPVGASGVAQVVEVWKQMHGTAGARQ
                     LPGELRYALTHNVGGTGQTTVVHIFEKRS"
     gene            complement(2746..3057)
                     /locus_tag="PKL60_07435"
     CDS             complement(2746..3057)
                     /locus_tag="PKL60_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96003.1"
                     /translation="MQDLIGTWAQIEGQPYPGLAFTFQPDGSFSATYELMSITSSGTY
                     KTEGELIEMNQTRHSFGLLGLFIGRYKVEGSRLLLNVVAAGAQERPADLNGAVVYEKV
                     A"
     gene            complement(3081..4400)
                     /locus_tag="PKL60_07440"
     CDS             complement(3081..4400)
                     /locus_tag="PKL60_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNW96004.1"
                     /translation="MAALDEYRAKRVSLEEAVSRIQSHQTVCVAMAGAEPPGLLEELG
                     RQAPRLEDVTTWTCLPMRGYDAFLKPEMEGRIFNENWFYGAPDRKAHPEGRVSYTPNN
                     LHSAAVDKLHATKNHVDVYIGTATPPNPEGYLSLSLGNVYEKDMVEVADLVILEVNEN
                     MPWTHGDTLVHISQVDLAVENNAALPMLINEEPTEIERLIGGHIADLIEDGATLQLGI
                     GGIPNAIASFLGSRKDLGLHTEMFVDGMVDLFQAGVVTNRRKTLHAGKMVATFALGSQ
                     KLYDFINHNPDVEILRGKYVNDPYVIGQNYRMISVNTAIQVDILGQVCSQSLGVRQFS
                     GTGGQADTHRGAQLSEGGRGIIALRSTAKNGTISTIVPTLAPGSGVTVTSQDVDTIVT
                     EYGIAELRGLSVRKRMEALIKIAHPDFRESIREEAHRLGIVPDKRYH"
     gene            complement(4415..5422)
                     /locus_tag="PKL60_07445"
     CDS             complement(4415..5422)
                     /locus_tag="PKL60_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNW96005.1"
                     /translation="MARYATILGTGHYIPENEISNATFAEWMGAVSPKLAEVVGKFEV
                     SSGIKTRFYAPEDWATSDLAVEAGKKALEDAGITPEQVDLVILGTDSPDYITPATSVV
                     VQYKLGCVNAGTWDVGCACASFPTALAQAAGMIATNPNLKYILVIGAYMMHKLADVKH
                     DITSFFYGDGGGAAVVGVSDKPGFVSCSIKADGSYHKHWGIYSGGTYEPATVESVEAG
                     RTTVKLLTPFPASVNHEGWPARARECAANGGFKIEDVDFVIFTQVNVNSIKLVMENLG
                     LPLEKAHWVMDKWGYTGSACLPIAFDDARKLGKIKSGDLVLFVGSGVGYNQAATAYIM
                     P"
     gene            complement(5452..6210)
                     /locus_tag="PKL60_07450"
     CDS             complement(5452..6210)
                     /locus_tag="PKL60_07450"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HNW96006.1"
                     /translation="MRLKDRVCVVTGGAAGIGKATALKFAQEGAKVVICDVNEEAGSA
                     FVKELGEAASFYKVNVTNRQEVQAWIDDVAAKYGRIDVLINNAGITRDAQFVKFKDGE
                     LVGQMSEEAFDMVIAVNLKGVFNCTQAVAPYMIKGGGGVIVNASSVVGLYGNFGQTNY
                     AATKFGVIGMTKTWARELGKYKIRVNAVCPGFILTDMVLKMPENVLAAMAAKSPLGRL
                     GTPEEIANTYLWLASDESSYVHGAALSVDGGVIP"
BASE COUNT         1290 a         1843 c         1800 g         1537 t
ORIGIN      
        1 ccttggttgg cagcacaatg ccgactgcca gtttttcacc agcggcgggt tcttcagcgg
       61 gggcttcagt tttcgcgcag gcgcccaaca ccatcgatgc gatgatgagg atcgagaaaa
      121 caaacattaa cgactttttc attctaagta ttctccttgt aagattgtgg tttgtcgttg
      181 tttcctaaca ggtttaagca tttcagtctc aggaacctcc ttgtttatcc ttgggttttt
      241 ggtttttcag cacaaaaccg atcagcagcg tgttcagcgg atcgggctta tccagacaca
      301 gggcatgccc tgagcccggc acaaccacat attctgcgcc ctggatttga tctacgatga
      361 tttgggcata ctcccgcccc ttgatcagat cgtccccccc caccacaacc agcgtggggc
      421 acttgatttt gggcagctcg ttggtgatat ccaacgcgta aaaggctttc attaactcaa
      481 caaaggacac cttgtccagc gcgccgtagt ttttaatcat ctctccagcg ctgcggcggg
      541 tcttggccac atactccgcg gaaaagttca ggtgcacgga agtccagtag agcatttcgg
      601 ggtcattgtg ttctgctgcc agctgccatg gataggtctg cgccgccagc atgggtttga
      661 tttcagagac cccgtcaatc acaatcagag accgggtttt ttcaggatat ttgagcgcaa
      721 aaaccatgct gatttctgag ccgtaagaaa ttccgccgat gtgcgcctgg tcaatcccca
      781 gaccatccag cagagctgcc aggtcgtcgg catgctgttc cagcgagtac ggtccggctg
      841 gatgttcgga gcgccacatc ccgcggcagt catagaggag cacgcgcatg tgcgcagcca
      901 gcgcgcgcgt ctggttaaac caactgccgg tgctcatcat gataccgttc gagagcacaa
      961 gtacttcccc gtcttcgggg ccgtgcagtt cgtaatacag ccgtacctta ttggcaagaa
     1021 tttctggcat tctttgtctc tgttttactg gatcggttca aaagcaagga acttttttgc
     1081 ttgtccctcg ggaccgcgtt caacgactgt catttttaac ggcgtaccaa tttttatttt
     1141 agcagggtct gtcaaatcca ttcccaggat ttgggcgctg atacgcggac cctcgtcaag
     1201 cgtgacaatc ccgaccatat atgggttttt gctgtcatat ccggctttga ccatctccgt
     1261 agcgggcaca taaatgaccg tgaaggctgc caacttacca gtcccgataa actcgatccg
     1321 ttcggcctgg gtagaatggc agcgcgagca aatgtagcgc tgggggatgc tgatgtgtcc
     1381 gcagttcagg cagcgcgaac cgatgagcac ctcattttca atcgcctgat agaaagtttt
     1441 ggtttcaagt ttgcagtcgc tcatctcagc tcctcttttc aaagatatgc actactgtgg
     1501 tctgaccggt cccgccaaca ttatgcgtta gcgcgtagcg gagctcaccg gggagctgac
     1561 gcgcaccggc agtaccgtgc atctgcttcc agacttccac aacctgcgca acgccggagg
     1621 cgcccaccgg atgacccttt gatttcaggc cgccgctggt attgatagga tgctcgccct
     1681 ggcgcgaggt tttcccttcc atggctgctt tccagccttc cccgcgcggg aaaaagccga
     1741 gatcctcgct cgccatgatt tccgcgatgg taaagcagtc gtgcacttcc gcgaagtcaa
     1801 tatccgcggc gctgatgcct gccatctcgt aggcttgcgc ggcagctttg gacgcagagg
     1861 gtacctcgga gagcgaatcc cgttccgcca gagcgccgtc gctggcctga ccaatgccgg
     1921 cgatagtaat cgggtgatcg gtgtattttg aggcgagctc ttcgctgacc agcagaaccg
     1981 cggcggctcc atccgagaca ggggagcagt caaacaagcg cagcggccag gcaatcatcg
     2041 ggttggcctt cggatcgtgc atgaagtcca tttcgttttc ccaggtaggt acgggcaggc
     2101 ctttttgttc agccttggcc tttttgcctg ccataacatc tttaatggtc tggttaaact
     2161 gagctttttc gttcagcgcg ccgttttcat ggttcttgat gcccacacac aggaaagcat
     2221 cggggtcggc ctgatattta tccatatagg cagtcgccat cgcggcgtaa aaaccaggga
     2281 aagtgaatcc ggctggaatt tcgaacattg tatccgcggc agaggcaagc gcgtctgtta
     2341 ccgcagcggt cggcaggtca gtcatctttt ccagaccgcc taccagcacc acgtcatact
     2401 gcccggaggc aatcgccagg atggcctggc gcagcgccgc gccgccgctc gcacaggcat
     2461 cttccatgcg cgcgctggca accggcgaaa tgccaatcca gctggcgaca atgggagcca
     2521 gatgcccttg tttctcgaac tggtcagagc tgaagttccc cagataaaaa gcttcgatat
     2581 ctttgggggg caggcctttt tcaacggagg agcgcagttc ctgataggct tccacgaaca
     2641 ggtcccgtga agatttatcc gggaacaccc cgaatttact catgccaact ccggcaactg
     2701 ccactccgcg tgctaatttt ttcccggtca tgctgtgtcc tttacttatg ctactttttc
     2761 gtagacaacc gcgccgttca ggtcagcggg gcgttcctgc gcccccgcgg cgaccacgtt
     2821 caacaataaa cgactgcctt ccactttata acgcccgatg aacaagccaa gcaatccgaa
     2881 actgtgccgg gtctgattca tctcaatcag ttcgccttcg gtcttatagg tgcctgagga
     2941 agtgatgctc atcagctcgt acgttgcgct gaaacttcca tcgggctgga aagtgaaggc
     3001 aagcccggga tagggctgcc cttctatttg tgcccatgtg ccaatcaaat cctgcatact
     3061 gttctcctgt ttcgccgcgc ttagtgatag cgtttgtcgg gcacgatgcc cagacggtgc
     3121 gcttcttcgc gaatggactc gcggaaatcc ggatgcgcaa ttttgatgag ggcttccatg
     3181 cgcttcctga cgctcagccc gcgcagctct gcgatgccgt actcggtcac tatagtgtcc
     3241 acgtcctggc tcgtaacggt cacgccggaa cccggcgcga gggtcgggac aatggtggaa
     3301 atggtgccgt tcttggcggt ggagcgcagc gcaatgatgc cgcgcccgcc ttcggacaat
     3361 tgcgctccgc ggtgggtgtc cgcctgaccg cctgtgccgc tgaactgacg cacgcccagg
     3421 ctctgggagc atacctgccc caggatatcc acctggatgg cggtattaac actgatcatg
     3481 cggtagttct gaccgatcac ataggggtcg ttgacgtatt tgccgcgcag aatttcaacg
     3541 tcggggttgt ggttgataaa gtcgtacagc ttctggctgc ccagcgcgaa ggtggcgacc
     3601 atcttgccgg cgtgcagggt cttgcggcgg ttggtgacaa ctccagcctg gaaaaggtcc
     3661 accattccgt ccacgaacat ttccgtgtgc agaccaaggt cttttcgtga gcccaggaag
     3721 gacgcgattg cgttggggat gccgccgatg ccgagctgca aggtggcgcc gtcttcaatc
     3781 aaatccgcga tgtgaccgcc gatgagacgt tcgatttcgg tgggttcctc attaatcagc
     3841 attggcagcg ctgcgttgtt ttccactgcc aggtccacct gggagatgtg taccagcgtg
     3901 tcgccgtgtg tccagggcat gttttcgttg acttccagga tgaccaggtc ggcaacttcc
     3961 accatatcct tctcgtacac gttgcccagg gaaagcgaga ggtagccttc cgggttcggc
     4021 ggggtggcag tgccgatgta aacgtccaca tggtttttgg tggcgtgcag tttatccacc
     4081 gcggcggaat gcaggttgtt gggcgtgtac gatacgcgcc cttcggggtg cgccttacgg
     4141 tccggcgcgc cgtagaacca gttttcgtta aagatgcggc cttccatttc cggcttcaga
     4201 aacgcgtcgt acccacgcat tggcaggcaa gtccaggtgg tcacgtcttc caggcgcggc
     4261 gcttgtctgc ccaattcctc gagcaaaccg gggggttcag cgccagccat agccacgcag
     4321 accgtctggt gcgattgaat tctggagacg gcctcttcca ggctgactcg tttagcgcga
     4381 tattcgtcca gggcagccat aactcctcgt agactcaggg catgatgtaa gcggtcgcgg
     4441 cctggttgta acccacgccg gagccgacaa agaggaccaa atcgccggat ttaatcttgc
     4501 ccagtttgcg ggcgtcgtcg aacgcgatcg gcagacaagc ggaaccggtg taaccccatt
     4561 tgtccatcac ccaatgcgct ttttcaagcg gcaggccgag attttccatc accagtttga
     4621 tgctgtttac gttgacctga gtgaaaatga caaagtcgac gtcctcaatt ttgaatccac
     4681 cgttcgccgc gcattcgcgc gcgcgcgccg gccaaccttc gtgattgacc gatgccggga
     4741 aaggcgtgag cagcttcacc gtggtgcgtc cggcttccac gctctccacc gtggcgggtt
     4801 cgtaggtgcc gccggaatag atgccccagt gcttgtggta agagccgtcc gctttgatgg
     4861 agcaggacac aaagccgggc ttatccgaaa cgccaaccac tgccgcgcct ccgccgtcgc
     4921 cgtagaagaa ggaagtgatg tcgtgtttca catcagcaag cttgtgcatc atgtaggcgc
     4981 cgattaccag gatgtatttc aggttcgggt tggtggcaat catcccggca gcctgcgcca
     5041 gcgcggttgg gaaagacgcg cacgcgcagc ccacatccca ggtgccggca ttgacgcagc
     5101 cgagcttgta ctgcacgacg accgaagtgg ccggggtgat gtaatccggg ctgtctgtgc
     5161 caagaatcac caggtcaact tgttctggag tgatgccggc gtcttcgagc gccttttttc
     5221 cggcttcgac tgccaggtcg gaggtcgccc aatcctccgg ggcatagaaa cgggttttga
     5281 tgccgctgct tacttcaaat ttgcccacca cttcggcgag tttggggctg accgcgccca
     5341 tccactccgc gaaggtggcg ttcgagattt cgttttcggg gatgtaatgc cctgtgccaa
     5401 gaattgtcgc gtaacgtgcc attgttgact cctgtcttta tcaccgatgc tttaggggat
     5461 gaccccgccg tccacgctca gggcagcccc gtgaacgtag gaggactcat cgcttgcaag
     5521 ccacaggtaa gtgttggcga tttcttccgg tgtgcccagg cgcccgagcg gggatttggc
     5581 cgccatggct gccagcacat tttcgggcat cttcaggacc atatcggtga ggatgaagcc
     5641 cgggcagacc gcgttgacgc ggattttata cttccccagc tctcgcgccc aggtcttggt
     5701 catgccaatg acgccgaatt tggtggcggc atagttggtc tgaccgaaat tgccgtagag
     5761 accgaccacg gaggaggcgt tgacgatgac gccgccgccg cccttaatca tgtacggcgc
     5821 gacagcctgc gtgcagttga agacgccttt caggttgacc gcgatgacca tgtcgaaagc
     5881 ttcttcgctc atctgtccga cgagttcgcc atccttgaac ttgacgaatt gcgcgtcgcg
     5941 cgtgatgccg gcattgttga tgaggacatc gatgcgcccg tattttgccg ccacgtcgtc
     6001 aatccaggcc tgcacttcct gccggttggt cacgttcact ttgtagaagc tggcggcttc
     6061 gccaagttct ttcacgaacg cgctgccggc ttcttcgttg acatcacaga tgacgacttt
     6121 cgcgccttcc tgagcgaact taagcgcggt agcttttcca atccctgcgg cgccgccagt
     6181 gacaacacaa actctatcct tcaaacgcat gctgatctcc tttaatacgc gggaataccc
     6241 aaatttaaat ctgttaatca ttttttaaca caaagggatt acagggcggt tacaaagcgg
     6301 taacaaaact gagtttttct gagtgaattc catctgtttg ttgggattaa aggcgccgca
     6361 atgaacctga tttcatcgca tgtgaggctt ggaacgcagg gattgtaaag atcccgtcgt
     6421 tgcgagcccc cgccgcgtga atcgcgccaa ggctaaaacc acattgaggg
//
LOCUS       DAOQAD010000102         6457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_14689_length_6457_cov_7.385973, whole genome shotgun sequence.
ACCESSION   DAOQAD010000102 DAOQAD010000000
VERSION     DAOQAD010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14689_length_6457_cov_7.385973"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            457..1059
                     /locus_tag="PKL60_07455"
     CDS             457..1059
                     /locus_tag="PKL60_07455"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNW96007.1"
                     /translation="MAEQKFLIVMRSGPIPGSSYFIEKDEVFLGRDLTNDLPVPDPEI
                     SRRHARFLRRGDAIYIEDLGSTNGTFLNGVRIAGPQQLNDGDLITLAENTVMSFEVKG
                     AATEPLKVGAAKAEPALYTPEAAAPAPAAYQPVSAPVPQQPIAPTPTPVRPKKRMGWC
                     LIVLLVLLALVLIVGVVLVFMPASWWCALSFNQLPGCPIY"
     gene            1306..3882
                     /gene="gyrA"
                     /locus_tag="PKL60_07460"
     CDS             1306..3882
                     /gene="gyrA"
                     /locus_tag="PKL60_07460"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNW96008.1"
                     /translation="MSEETELPEVPEDNSAGNLEHVDIDSQMRTAYLDYAMSVIVARA
                     LPDVRDGLKPVHRRILYAMLDMGNRSNAPYKKSARIVGEVLGKYHPHGDSAVYDAMAR
                     MAQDFSMRYPLVDGQGNFGSIDGDSPAAMRYTEARLAKTAEELLLDLDKQTVDFVDNF
                     DGSLQEPEVLPARLPNLLMNGSNGIAVGMATNIPPHNLRELTAALNILIDRYDEIDEV
                     SVEDLMQAVPGPDFPTGGIIVGGEGIRQAYSTGRGKLTVRGVAKIEEIRAGRFAIIIT
                     EIPYQLNKTSLIERIAELVRAGKLDAISDMRDESDRRGLRIVIELKRGTQPKKVLNQL
                     YKFTPLQSTFGVQLLALVDGEPRMLSLKRALFHYLEHRQVVITRRALFELDKAKKRAH
                     ILDGLLIALANLDAVIQTIRSSPDADTAKTNLMERFTLTEIQAQAILDLQLRRLAALE
                     RQKIEDEHKELMLQIADLEDLLAHPRKILEVIRTDLNEVAEKFGDERRTRIDHDLEND
                     LSDEALVQDTPVFVSITRRGYIKRVSDGAYRAQGRGGRGVMGQTMRDEDEVHFFVRSN
                     TLATLLFFTDKGKVYSERVFELPEESRQGKGIALVNIINLEPDENVTALLSVPDFKQA
                     DFCTMATRRGRVKRIALAEFANVRPSGLIAIKLEEGDHLGWVCLTSGKDDVLLVTRGG
                     KALRFSEQEIRPTGRTTMGVSGIHMRAQDALAAMDVVEPGAYLLVLTEKGHGKRTPLE
                     EYMPKSRATLGVATISPTALARIGQIAEALVVQESDEITLISSAGIVLRMKAVNVSVQ
                     GRATQGVKVMVLDEGDSLAAVARIPALTANPDADADADADLNGDANPPASEGAQPDIS
                     AE"
     gene            complement(3858..4610)
                     /locus_tag="PKL60_07465"
     CDS             complement(3858..4610)
                     /locus_tag="PKL60_07465"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNW96009.1"
                     /translation="MDSGPLPRAFRHILLPETGSTNDDCLKLADAGAPDGTLVRALHQ
                     TGGRGRGSRSWFSRPGASLTFSVLLRPEAREYAHLTRFALLGCLALARALSRNFSAEA
                     QIKWPNDVLLSGKKVCGVLTEALWQDQLPSALVLGMGVNLTDEALPPQSETIFPAGSL
                     AGQAGIQVSAQALLEVLLPELARAREELPCPGFLAEVNARLACRGEWVSLRSNADAPF
                     RARPISVEADGSLWAEGEDGRMARFYSAEISG"
     gene            complement(4618..6165)
                     /locus_tag="PKL60_07470"
     CDS             complement(4618..6165)
                     /locus_tag="PKL60_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNW96010.1"
                     /translation="MLPDYRVRQRDYLLEISRALTEELDLDALLLHILRIAIEMLTGH
                     AGFIALKDDKRGWHIAVHEGIAEALLNYLQRWLEAQPVEGEDTSEAHVPEINRMLNDI
                     SMGMLTGVGINMIFQKQVIGQIYVFRNYRGLFSTNDRMLLTSFANQAAIAVRNARLYN
                     ETREQSLRLEALLDSAADGILILTPDLKVERMNKALQRLLNTSEEACIGKPSAEVIRW
                     AKPPQGTRLETAVASGWAQNGQNVFYLEGDLVREGLREPLPVGISYGPLFSADDALMN
                     IIASVRDITRFRTAEEMKTSFISVVSHELKTPIALIKGYVSTLRRDDVEWDREIVSES
                     LAVIEEEADRLTEMVEDLLDATRLQAGGLTLKKSELSIPQLARFLADRYTAQTDQHSF
                     VVDFPPDFPALALDEERIRQVLTNLLNNAMKYSPDGEIRISGKLRPKDVVVCVSDRGP
                     GIDPRDLPYVFERFYRSDEAAKTTKGTGLGLYLCREIIRAHEGKIWVDETYKEGSRIC
                     FSLPRDQ"
BASE COUNT         1315 a         2029 c         1876 g         1237 t
ORIGIN      
        1 ttgtggggct gtcgttgggt ttcgctcctg cgtcgctgca cccaacctac aaactaagta
       61 ccatcctttc tcaggtctgt catcctggcc gccagcgaaa aaccttcttg aaggctggca
      121 ggctgtcatt ctgagcgcca gcgaagaatc tatcttacaa catcgtagat tcttcacttc
      181 gttcagaacg acaactgttt ttcgtcaact tgagcgcgga ggcctgactg cggatgttgg
      241 gttgcgctcc tgcgtcgctg cacccaacct acaaactaag taccatcctt tctcaggtct
      301 gtcatcctgg ccgccagcga agaatctcgc cgccgaaacg ggcagctcct tcacaggctc
      361 aggataaccc gccctccccc ctgattgaca tccgcgccca aaatcagtaa actataactg
      421 ttaagcattc cgacccctga cccaaaggag cacaccatgg cggaacagaa attccttatc
      481 gttatgcggt ccggccccat ccccggctcc agctatttca tcgagaaaga cgaagtcttc
      541 ttaggccgcg acctgaccaa cgacctgccc gttccggacc cggaaatctc ccgccggcac
      601 gcccgcttct tacgccgcgg ggacgcaatc tacatcgaag acctcggctc caccaacggc
      661 accttcctga acggcgtgcg tattgccggc ccccagcagc ttaacgacgg cgacctgatc
      721 accctggcgg aaaatacggt gatgagcttt gaagtgaaag gcgctgctac cgaaccgctg
      781 aaagtaggcg ccgccaaagc cgagcccgct ctctataccc ccgaagccgc agcccctgcc
      841 ccagctgcct accagccggt ttccgcgccc gtgccgcagc agccgattgc cccgacccca
      901 acccccgtgc gcccgaagaa gcgcatgggc tggtgcctga ttgtgctgct cgtcctgctg
      961 gcgctcgtgc tgattgttgg cgtagtgctg gtattcatgc ccgcctcgtg gtggtgcgcg
     1021 ctttccttca accaactccc cggttgcccg atttattgag cgccttcgca taaacatctc
     1081 cgcaggctga tgaattcatc agcctctttt tttgtcgccg cggactcttc gcaaaatttc
     1141 agcaaaaaat cacagcttgc ctccgcctca gggttttccc attaaccgcg cctgcattgg
     1201 aaaaaactat ggatttttgg cgctaaagta aaaagccggc tttcagggta taatagtaag
     1261 gttgccgggc aaaccaaaat ccgccgggca gcatcaggat gaggaatgag cgaagaaacc
     1321 gaactccccg aagtgccgga ggataacagc gccggaaacc tggagcatgt ggatatcgac
     1381 agccagatgc gcaccgccta tctggattac gccatgagcg tcatcgtcgc gcgcgccctg
     1441 ccggatgtgc gcgacggcct gaaaccggtc caccgccgca ttctctacgc catgctggac
     1501 atgggcaacc gctccaacgc gccctacaaa aaatccgcca gaatcgtcgg cgaggtgctg
     1561 gggaaatacc atccccacgg cgactcggct gtgtacgacg caatggcgcg catggcgcag
     1621 gacttttcca tgcgctaccc cctcgtggac ggtcagggca actttggctc catcgacggc
     1681 gactcaccgg ctgccatgcg ttacaccgaa gcccgcctgg ccaagactgc cgaagagcta
     1741 ctgctggacc tggataaaca aaccgtggac ttcgtcgata atttcgacgg ttcactgcag
     1801 gagccggaag tgctgcccgc gcgtttgccc aatctgctga tgaacggctc caacggcatc
     1861 gcggttggca tggccaccaa tatccccccg cacaacctgc gcgaactcac tgccgccctc
     1921 aacatcctca tcgaccgcta tgacgaaatc gacgaggtca gcgtggagga cctgatgcag
     1981 gcggtccccg ggccggactt cccgaccggc ggcatcatcg tcggcggcga gggcatccgc
     2041 caggcataca gcaccgggcg cggaaaactg accgtgcgcg gcgtggcaaa gattgaggaa
     2101 atccgggcag ggcgctttgc gattatcatc acggaaatcc cctaccaact gaacaaaacc
     2161 agcctgattg aacgcatcgc cgaactggtg cgcgccggca aactcgacgc aatttcggac
     2221 atgcgcgacg aatccgaccg gcgcggttta cgcatcgtga ttgagctcaa gcgcggcacc
     2281 cagcccaaaa aggtgctcaa ccagctttac aaattcaccc cactgcagtc caccttcggc
     2341 gtgcagctgt tggccttggt ggacggcgaa ccccgcatgc tttcgctcaa acgcgcgctc
     2401 ttccattatc tggaacaccg tcaggtggtc atcacccggc gcgcgctctt cgagttggac
     2461 aaagccaaaa aacgcgccca catcctggac gggctgctca tcgcgcttgc caacctggat
     2521 gcggtcatcc agaccatccg ctcctccccg gatgctgaca ccgccaagac caacctgatg
     2581 gagcgcttca ccctgacgga aatccaagcc caggccatcc tggacctcca gctgcgccgg
     2641 ttggccgcgt tggaacgcca gaagattgaa gacgaacaca aagaactgat gctgcaaatt
     2701 gccgacctgg aagatttgct ggcccatccc cgcaaaatcc tcgaagtcat ccgcacagac
     2761 ctgaacgagg ttgccgaaaa attcggcgat gagcgccgca cgcgcatcga ccacgacctg
     2821 gagaacgacc tttctgacga agccctggtg caggacacgc cggtgttcgt ctctatcacc
     2881 cggcgcggct atatcaagcg cgtgtccgac ggcgcctata gggcgcaggg gcgcggcggg
     2941 cgcggcgtga tgggacagac catgcgggac gaggacgagg tacacttctt tgtgcgcagc
     3001 aacacgctgg ccaccctgct gttcttcacg gacaagggca aagtgtactc cgagcgcgtg
     3061 ttcgagctgc cggaagaaag ccggcagggt aaaggcatcg cgttggtcaa catcattaac
     3121 ctggaacccg acgagaacgt caccgcgctg ctttccgtgc cggatttcaa gcaggctgac
     3181 ttttgcacca tggccacccg ccgcgggcgc gtcaagcgca tcgcgctggc ggaattcgcc
     3241 aacgtgcgtc cctccgggct tatcgccatc aaattggaag aaggcgacca tttgggttgg
     3301 gtctgcctca ccagcggcaa ggacgacgtg ctgctcgtca cgcgcggcgg gaaggcgctg
     3361 cgcttttccg agcaagaaat ccgtccgacc ggccgcacca ccatgggcgt cagcggcatt
     3421 cacatgcgcg cccaagacgc gctggctgcc atggacgtgg tggaaccggg cgcttacctg
     3481 ctggtgctga ccgaaaaagg gcacggcaag cgcaccccgt tggaagaata catgccaaaa
     3541 tcccgcgcga ccctcggcgt cgccacgatt agtccgactg cgttagccag aattggtcaa
     3601 atcgcggaag cgctggttgt gcaggaatca gacgaaatta ccctgatttc ttctgccggg
     3661 attgtgctgc gcatgaaagc cgtcaacgtt tcggtgcaag gccgggcaac gcagggtgtc
     3721 aaagtgatgg tcctggacga aggggacagc ctggcagcag tcgcgcgcat ccctgcacta
     3781 actgccaacc cggatgcgga tgcggacgcg gacgccgacc tgaacgggga tgcgaaccct
     3841 cccgcgagcg agggcgctca gccggatatc tcggcggagt aaaagcgcgc catccggccg
     3901 tcttcccctt ccgcccataa actgccgtct gcttcgacgg atatcggacg cgcccgaaag
     3961 ggcgcgtctg cgttcgaacg caagctcacc cactcccccc ggcaagccag gcgcgcgtta
     4021 acctccgcaa ggaatccggg gcagggcagt tcttcccgcg cgcgggcaag ctcgggcagc
     4081 agcacctcca gcaaggcttg agcggagacc tgaatgccgg cctgtcctgc caggcttccg
     4141 gccgggaata tggtttcgga ttggggcggc agcgcttcgt cggtaaggtt tacccccatc
     4201 ccgagcacaa gcgcgctggg aagctggtcc tgccagagcg cttcggtcag caccccgcat
     4261 acctttttcc ccgaaaggag cacatcgttc ggccacttaa tttgggcttc agcggagaaa
     4321 ttgcgggaaa gcgcgcgcgc gagcgccagg cagcccagca gcgcgaagcg cgtcaggtga
     4381 gcatattccc gcgcttcggg gcgcagaagc acgctgaagg tcagcgaagc ccccgggcgg
     4441 gaaaaccagc ttctgctgcc gcgtccgcgc ccgccagttt gatgcagcgc gcgcaccagc
     4501 gtgccgtccg gagcgccggc atccgccagt ttaaggcagt cgtcattggt ggagccggtc
     4561 tccggaagga gaatgtgtcg gaatgcgcgc ggcaggggtc cggaatccat cgcagcctta
     4621 ttggtcgcgc gggagcgaaa aacagatgcg agagccttct ttataggtct cgtccaccca
     4681 gattttgccc tcatgcgcgc ggatgatttc ccggcacaga tataaaccca acccggtccc
     4741 tttagtggtc ttggcggctt cgtccgagcg gtagaagcgc tcgaacacat atggcaaatc
     4801 gcgcggatca atgcccgggc cgcggtcgct gacgcacacc acgacgtcct tcgggcgcag
     4861 tttgccgctg atgcggattt cgccgtccgg gctgtacttc attgcgttgt tcagcaggtt
     4921 ggtcagcacc tgccggatac gctcttcatc cagcgcgagc gcgggaaaat caggcgggaa
     4981 gtccaccaca aagctgtgct ggtccgtctg agcggtgtag cggtcggcca aaaagcgcgc
     5041 cagctgcggg atggaaagct cggatttctt cagggtcagc ccgcctgcct gcaggcgcgt
     5101 cgcgtccagc aggtcttcca ccatttcggt caggcggtca gcttcttcct cgattaccgc
     5161 caggctctcg gagacaattt cacggtccca ctccacgtca tcccgccgca gcgtgctgac
     5221 gtaacctttg atgagcgcga taggggtctt gagctcgtgg ctgacaacag aaatgaagct
     5281 ggtcttcatc tcttccgcgg tgcggaagcg cgtgatatct cgcaccgagg cgatgatgtt
     5341 catcagtgcg tcatcggcgg aaaaaagcgg cccgtagctt atcccgacag gcagcggctc
     5401 gcgcaaacct tcccggacca ggtcgccttc caggtagaaa acattctggc cgttctgcgc
     5461 ccagccgctg gcaactgccg tttccagtct ggtaccttgc ggaggctttg cccagcggat
     5521 aacctcggca gaaggcttcc caatgcaggc ttcctcgctg gtattcagca gccgctgcag
     5581 ggctttgttc atgcgctcca ccttcaagtc cggcgtgagg atcaggatgc cgtctgccgc
     5641 cgaatccagc agggcttcca ggcgcaggga ttgctcgcgg gtttcgttgt acaggcgcgc
     5701 attgcgcacg gcaatggctg cctggttggc aaagctggtc agcagcatgc ggtcgttggt
     5761 ggagaagagt ccgcggtaat tgcggaacac gtaaatttgc ccaatgacct gcttctggaa
     5821 aatcatgttg atgcccacgc cagtcagcat tcccatgctg atatcgttca gcatgcggtt
     5881 gatttcaggt acgtgcgctt cgctggtatc ttccccttcc acgggctgcg cttccagcca
     5941 gcgctgcagg taattgagca gcgcttcggc aatgccctcg tgcacagcga tatgccagcc
     6001 gcgtttgtcg tctttcagcg cgatgaagcc ggcgtggccg gtcagcatct caattgctat
     6061 ccgcaggatg tgcagcagca gcgcgtccag gtccagctct tcggtgagag cgcgggaaat
     6121 ctcgagcagg taatctcgtt ggcgtacgcg gtagtcgggg agcatggttc cattttaacc
     6181 gcaaatcatc aaaaagagcg gcgctgtaaa ggaaggtggg ctctggctgc gagcttaacg
     6241 cagtcgtagg tcgggtgaag cggcagcgca acccgacaga acgtggtcgt aggtcgggtg
     6301 aagcggcagc gcactccgac aagctttggc tgtcgggttt gtggagctgt cgttgggttt
     6361 cgctcctgcg tcgctgcacc caacctacaa actggcagtt tttcatccag tccgtggtcg
     6421 taggtcggct ggagaagcaa tggtcgtagg tcgggtg
//
LOCUS       DAOQAD010000103         6441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_14748_length_6441_cov_5.841685, whole genome shotgun sequence.
ACCESSION   DAOQAD010000103 DAOQAD010000000
VERSION     DAOQAD010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14748_length_6441_cov_5.841685"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="PKL60_07475"
     CDS             <1..1434
                     /locus_tag="PKL60_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW96011.1"
                     /translation="SNKYDRSQLNPYVWGVYDGYEDLYPELVDPDNGILKEYDNLANM
                     RQYALLFNYQMTAAGNPFILSGALDGSGIPPDFFSDIHVRKAFSYAVDWQPVIDTAYG
                     AQAIRAQGPIPMGEIGYDTALQPYPFDLALAEDELQLAFGGALWTNGFMMILPIWSNQ
                     AFNNLANQLKNNLESIDPAKIHIQITQFSYEEMIGYRNAGFTPLWWSGWLEDYHHPHN
                     WMMAYLLSDAPFGYGGIQHFPPALATLFESTINYCVAVSDPTEMIDCYQNIQGLSHTY
                     AAAMWGIQVKYTDYLRAEVRGYYYNPALIPMPIYELSKGAVPTTFTVEPGSQASLDFD
                     FPSGGSLNILLPAGALAEPIAIVFTPDTDVDERMPGGLFRSGKRFHLMVCQENQCDEP
                     YFFEDFVTLTLQYTDADVQSLIEDELYLYVWDGSYWVDIVQQCSAGGMQYIRDPSQNR
                     IGAPVCHFSRFVMNGEAHTQFLPVLRK"
     gene            1648..2301
                     /locus_tag="PKL60_07480"
     CDS             1648..2301
                     /locus_tag="PKL60_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNW96012.1"
                     /translation="MISTGDMLLRLAVSMLLGGAIGFERERDSQPAGLRTHMILVLGS
                     CLAMILSVNMSAAAGSDPARLAAQVVSGIGFLGAGAILRSGFNVKGLTTATTVWTTAI
                     IGLAVGYGYYWVGVFTTVLVLIVLTVVSLIEKKFIRKNVIRVINVDATDNPHIFRAVR
                     KEISRNAEQIVSYKTQKSLKSGHVRVEFVARLERAEKIEDLMETLSKIDGVRNIRIE"
     gene            2362..4005
                     /locus_tag="PKL60_07485"
     CDS             2362..4005
                     /locus_tag="PKL60_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96013.1"
                     /translation="MPGILHCLSGHDLGFLRSAADLWGLNLSAKDAPAYGRALASALA
                     QHPELEEIAAALPDESRAALAALQSRGGSLPWAAFTRQYGDLRQMGPARRAREKPHFF
                     PQSTTERLWYRALIGRDFFRQGDDLVELAYIPDEFLERLPVFPEDAEPSARLEPEKQV
                     SVERALFWQDRILDESCTLLAALRRFGTPEVLREAARPGIDWELLRCLLGSLGIIDRD
                     GKTPAPEARRFLEMPRGQSLQWLAAAWMNSIRFDELRLAPGLVCQGTWPHNPLPARRA
                     LLHQLAGLENGAWYAIDAFSAELRRENPEYLRAGGDANEWIILSAKDGRLFRRPEDWL
                     EVEGAWTGFLIRGLLFWLGFVELGFAAGAEEPAYFRKSNWCAALLSSREPLALPAENS
                     PAAVSSTGLISLNRGAPRTARYQLARFSEWLENGPERWQYLLTPASLSAAAEQGLNMR
                     HLSALLRKYAKPAPPPSLMGALARWETNGREASIERLWVLRLRSPEALQALRESPAAG
                     FLGEALGPASVIVHPGGLEKVRAALARLGTLSDVEELLK"
     gene            4002..4439
                     /locus_tag="PKL60_07490"
     CDS             4002..4439
                     /locus_tag="PKL60_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96014.1"
                     /translation="MTAEPARTPPGWLNALVRLMLLFHLASGVLRANGALAQKDLLLE
                     LGLPARLPGYLLAAGLLGALLSLIALVFTWRPGRLSLIALWAALTFAIGGYWVERLFL
                     WAPEQRGGSPLFKLGLQALSVAAAALYTYHIKKRSQNAHGPGN"
     gene            4423..5049
                     /locus_tag="PKL60_07495"
     CDS             4423..5049
                     /locus_tag="PKL60_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNW96015.1"
                     /translation="MDQEIEVKFSLANPGRLRQRVIDAGAKLLQARVFEQNLRFDTPE
                     MRLRERAQVLRLRADQAAHLTFKGPGQVREGVLERQEIEFEVSDFGAARRLLEALGYR
                     VYTSYEKYRETYRMGGVLIVLDELPFGSFAELEGPSAKEIHAAADALGLDWNKRLNTS
                     YMDLFYALKERLGLDAEALSFEAFRGLTLRPDDLGAQPADLRDGKDSL"
     gene            5046..5576
                     /locus_tag="PKL60_07500"
     CDS             5046..5576
                     /locus_tag="PKL60_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96016.1"
                     /translation="MKLWKTQSRDIILDRKPRLAVEKRHVELPDGTQNEDWLWLDAPE
                     YAVVTAVDANGRFVTLEQHPYAVHGPLPAPVEGLIRAGEEAEDAAKRLLLQQAGYSSP
                     DWVFLGDFITDAESGYGRGYYFLARLAVPAAEGADPLVRLLSREQLQDAVAEGQPRVL
                     TWQTAFSLALLRLFLR"
     gene            complement(5573..6415)
                     /gene="murI"
                     /locus_tag="PKL60_07505"
     CDS             complement(5573..6415)
                     /gene="murI"
                     /locus_tag="PKL60_07505"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNW96017.1"
                     /translation="MNAASSPSQPVALYDSGVGGLSVLRAAQAALPHERLLYFGDQAN
                     VPYGSRSLEELRAIAEASTRFLLSQGAKLIVIACNTASAAALKHLRAAFPDVPFVGME
                     PAVKPAAEQSLTRKVGVLATPSTFQGELYASVVERFAQDVEIFQSTCPGLVSQIERAR
                     LNTPKTRRILEEAVLPMLDAGADTLVMGCTHYPFVIPLIEKIAAGRARVIDPAPAVAR
                     QTARVLAERKLLNPSPEEGSLRFITSGEPAAFEYALARLLGLNAQAEAARWQGGELKL
                     IPRG"
BASE COUNT         1185 a         1910 c         2061 g         1285 t
ORIGIN      
        1 tcaaacaaat atgatcgcag ccaacttaac ccctatgtct ggggcgttta cgatggatac
       61 gaggacctgt acccggagct tgtggacccg gataacggaa tcctgaaaga atacgacaat
      121 ctggcgaata tgcgccaata cgcgctgctg ttcaattacc aaatgaccgc ggctggcaat
      181 ccctttattc tcagcggcgc gctcgacggc agcggcattc cgcctgattt cttctcggat
      241 atccacgtgc gcaaagcctt cagctatgcc gtggattggc agccggttat cgacactgcc
      301 tacggcgcac aggccatccg ggctcaggga cccattccca tgggggaaat tggttatgac
      361 accgccctgc agccttatcc gtttgacctc gcgctggctg aggatgagct ccaactggcc
      421 tttggtggtg cgctctggac aaacgggttt atgatgatcc tgccaatctg gagtaatcag
      481 gcttttaaca acctggctaa ccagttaaaa aataaccttg aatcaatcga cccagcaaag
      541 attcatatcc agattaccca attctcttat gaggagatga ttggttaccg caatgccggc
      601 tttacaccgc tttggtggtc gggttggttg gaagactacc accacccgca taattggatg
      661 atggcgtatt tgctttccga cgcgcctttt gggtatggag gcatccagca ttttccgcct
      721 gcactggcga ccctttttga atcaacgata aattactgcg tcgctgtttc tgacccaacc
      781 gagatgattg actgttatca aaatatccag gggctttcgc acacctacgc ggctgccatg
      841 tgggggatcc aggtcaaata taccgactat cttcgcgcgg aagtgcgcgg atattattac
      901 aatccggcat taatcccgat gccaatttat gaattgtcca aaggcgccgt gccgaccaca
      961 ttcacggttg agcccgggtc gcaggcatcc ctggattttg attttccatc cggaggcagc
     1021 ctgaacattc tgctgcccgc aggtgcgctg gcggagccaa tcgcgattgt tttcacgccg
     1081 gatactgatg tggacgaacg catgccggga gggttgttcc ggagtgggaa gcggttccat
     1141 ttgatggtct gccaggaaaa tcaatgcgat gaaccgtatt tctttgagga ctttgttacc
     1201 ctgaccctgc agtataccga cgcggatgtt cagagtctca ttgaggatga actctatctg
     1261 tacgtgtggg acggcagcta ttgggtggat atcgtccagc agtgcagcgc gggggggatg
     1321 cagtacatcc gtgacccttc ccagaacaga atcggagccc cggtttgcca tttttccagg
     1381 tttgtgatga acggagaggc gcatacccag ttccttcctg tcctgcggaa atgagccggg
     1441 cacctctccc tacaacgaac cgcttgactt acgccgcccg gtaaaaacag ggcggtgtat
     1501 ttcgttggaa ggtgtgtcaa gcaaaaggga gcaggccggt ttttcaaggc tcacctaagc
     1561 atgttacaat ttcgtatcaa agctggcgaa gccagccgcg aatgctcatc tctgctctta
     1621 tgattaagga ttgatgcgag gatctcgatg atatctactg gcgatatgct cttgcgtttg
     1681 gcggtctcca tgctgctggg cggcgcgatc ggcttcgagc gcgaacgcga cagccagccc
     1741 gcgggtttgc gcacgcatat gatcctggtg ttgggctcct gcctggcgat gatcctctcg
     1801 gttaacatga gcgctgccgc cggttccgac cccgcccgcc tggcggcgca ggtcgtctcc
     1861 ggcatcggct ttttaggcgc cggcgccatc ctgcgctccg gctttaatgt caaaggcctg
     1921 accactgcca ccacggtctg gaccaccgcg attatcggtt tggcagtcgg ttacggctac
     1981 tactgggtag gggttttcac caccgtgttg gtcttgattg tgctgaccgt ggtgagcctg
     2041 attgaaaaga aattcatccg caagaacgtc atccgcgtca tcaacgtgga cgctacggat
     2101 aacccgcaca tcttccgggc tgtgcgcaag gaaatcagcc ggaacgccga acagattgtg
     2161 tcgtacaaga cccaaaagag cctgaagagc gggcatgtgc gcgtggagtt cgtcgctcgc
     2221 ctggagcgcg ctgagaaaat tgaagacttg atggaaaccc tgtctaaaat tgacggggtg
     2281 cgcaacatcc ggattgagtg acgccgcgcg ccgcaaagat ctgggaggaa ggcaagtttt
     2341 tgatgtatac tccactgcac gatgcccggt atactgcact gcctgagcgg tcatgacctt
     2401 ggttttttgc gcagcgccgc cgatttgtgg ggtctgaacc tgagcgcgaa agacgcgcct
     2461 gcctacggac gggcgttggc ctccgcgctg gcccaacacc ccgagctgga agagattgcc
     2521 gctgccctgc cggatgagag ccgcgccgcg ctggccgcct tgcagagccg cggcggcagc
     2581 ctgccatggg cagcctttac gcgccagtac ggcgacctga ggcaaatggg cccggcccgc
     2641 cgcgcgcgcg aaaaaccgca cttctttccc cagtccacca ccgaacgcct gtggtaccgc
     2701 gccctcatcg gccgggattt ttttcggcag ggcgacgacc tggtggagct ggcctacatc
     2761 cccgacgaat ttctggagcg cctgcccgtc tttccggagg atgccgagcc ttccgcgcgg
     2821 ctggagcccg agaaacaagt cagcgtggag cgcgcgctct tttggcagga ccgcatcctg
     2881 gatgaaagct gcaccctgct cgcggcgctg cggcgctttg gcacgcctga ggttttacgc
     2941 gaagcagcgc gcccgggaat cgattgggag ctcttgcgct gcctgctcgg cagcctgggc
     3001 attatcgacc gggacggcaa gacccccgcg ccggaagcgc gccgcttttt ggagatgccg
     3061 cgcggccaaa gcctgcagtg gctggcagcg gcttggatga actccatccg cttcgacgag
     3121 ctgcgcctgg cgcccggcct tgtctgccag ggcacctggc cgcataatcc gctgccggcg
     3181 cgccgggcct tgctccatca gctggccggg ctggaaaacg gggcgtggta cgccatcgat
     3241 gcctttagtg cggagctgcg ccgcgaaaac ccggaatacc tgcgcgcggg cggggacgcc
     3301 aacgaatgga ttatcctcag cgccaaagac ggcagacttt tccgtcgacc ggaggattgg
     3361 ctggaggtcg aaggcgcctg gaccggcttc ctcatacgcg ggctgttgtt ctggctgggt
     3421 tttgtggaac tgggctttgc ggccggcgcg gaagaacccg cgtacttccg caaatccaat
     3481 tggtgcgccg cgctgctcag ttcgcgcgag ccgctggcgc tgcccgcgga gaacagcccc
     3541 gcggcagtca gctcaaccgg gcttatcagc ctgaaccgcg gtgcgccgcg gaccgcccgc
     3601 taccagctgg cgcgcttctc ggaatggctg gagaacggac ccgaacgctg gcagtacctg
     3661 ctcacgcccg cttccctttc cgccgccgcg gaacagggct tgaacatgcg ccacctgagc
     3721 gccctgctgc gcaaatatgc caaacccgcc ccgccgccca gcctgatggg ggcgttggcg
     3781 cgttgggaaa caaacgggcg cgaggcttcg attgagcgct tgtgggtgct gcgcctcagg
     3841 tcgcctgaag cgctgcaggc gctgcgcgaa agccctgccg cgggctttct gggcgaagcg
     3901 cttggaccgg cctccgtgat tgtgcacccg ggcgggctcg agaaggtgcg cgcagcgctg
     3961 gcgcggctgg gaactctcag cgatgtggag gagctgctca aatgaccgct gaacctgcca
     4021 ggacccctcc cggctggctg aacgcgctgg tgagactgat gctgctcttc catctggcga
     4081 gcggcgtttt gcgagccaac ggcgcgctgg cgcaaaaaga cctgctgctg gagcttggcc
     4141 tgcccgcgcg gctgcccgga tatctgttgg cggctggctt actgggggcg ctgctgagcc
     4201 tgattgcgct ggtattcacc tggcggcctg ggcgcctgag tttgattgcg ctgtgggccg
     4261 cgctgacctt tgccattggc ggctattggg tagagcgttt gttcctgtgg gcgcccgaac
     4321 agcgcggagg gagcccgctc ttcaagctgg gtctacaagc gctctccgtg gcagccgcgg
     4381 cgctttatac ttaccatatc aaaaagagga gtcagaacgc gcatggacca ggaaattgaa
     4441 gtcaagtttt cccttgccaa ccccgggcgt ttgcgtcagc gcgtgattga cgccggcgca
     4501 aagctcctcc aggcgcgcgt attcgagcag aacctgcgct tcgacacgcc tgaaatgcgc
     4561 ttgcgcgaga gggctcaggt gctgcggctg cgcgcggacc aggctgccca cctcactttc
     4621 aagggtcccg ggcaggtgcg cgaaggggtc ttggagcggc aggaaatcga gttcgaagtt
     4681 tccgattttg gtgccgcccg gcgcctgctg gaggctctgg gttaccgcgt ctataccagc
     4741 tacgaaaagt accgtgagac ttatcggatg ggcggcgtgc tcatcgtgtt ggacgagctg
     4801 ccgttcggct cctttgccga gctggaaggc ccctccgcga aggaaattca tgccgccgcg
     4861 gacgcgctgg gcctggactg gaacaaacgc ctgaacacca gctatatgga cttgttttac
     4921 gcgcttaagg agcgcctggg cctggacgcg gaggcgctca gcttcgaagc cttccgcggg
     4981 ctgacgctgc ggccggatga tttgggcgcg cagccggcgg atttgcgcga tggaaaggat
     5041 tcgttatgaa actctggaag acacaatcac gcgatatcat tctggaccga aaacccaggc
     5101 tggcggtgga aaaacgacat gtggaactgc ccgacggcac ccaaaacgag gactggctgt
     5161 ggctggacgc acccgaatac gcggtggtga ccgcggtgga cgcgaacggc cgcttcgtca
     5221 ccctggagca gcatccctac gccgtgcatg ggcccctgcc cgccccggtg gaaggcctca
     5281 tccgcgccgg ggaagaagcc gaggacgccg ccaaaaggct gctgctgcag caagccggct
     5341 acagctcccc ggattgggtc tttctggggg atttcatcac ggacgcggaa agcggctatg
     5401 ggcgcggtta ttacttcctg gcgcgcctgg cagtgcccgc cgcggaaggc gctgacccgc
     5461 tcgtccggct gctctcgcgc gagcaattgc aagacgccgt agcggaaggc cagccgcgcg
     5521 tgctcacctg gcagaccgct ttttcgctgg cgctgctcag gctctttttg cgttagccgc
     5581 gggggattag cttcagctcg ccgccctgcc agcgcgccgc ttcggcctgg gcattcaatc
     5641 caagcaaacg cgcgagcgcg tattcaaacg cggcgggttc tccgctggtg ataaagcgca
     5701 ggctgccctc ttcgggagag ggattcaaga gcttgcgttc ggccaaaacg cgcgcggtct
     5761 gtcgcgccac ggcaggggcg gggtcaatca cgcgggcgcg cccggcggcg attttttcga
     5821 tgagcgggat gacgaagggg taatgcgtgc agcccatcac cagcgtatcc gcgccggcgt
     5881 ccaacatagg caggacggct tcttccagga ttcggcgggt tttgggcgtg ttcaggcgcg
     5941 ccctttcgat ttggctcacc agcccggggc aggtggactg aaaaatttcc acatcctgcg
     6001 cgaagcgttc caccaccgaa gcatacagct ccccctggaa ggtggagggc gtggccagca
     6061 ccccgacctt gcgggtgagg gactgctcgg cggcgggctt gacggccggc tccatcccca
     6121 cgaagggcac gtccgggaag gccgcgcgca ggtgcttgag cgccgcggcg gaggcggtgt
     6181 tgcaggcgat gacgatcagc ttggcgccct gggagagcag aaaacgggtg gaggcttcgg
     6241 cgatggcgcg cagttcttcc agcgagcggc tgccgtaggg cacgttggcc tggtcgccga
     6301 aatagagcag gcgctcgtgg ggcagggccg cctgggccgc gcgcaagacg gagagcccgc
     6361 ccacgccgga gtcgtacagg gcgacgggct gggaggggga ggatgcggcg ttcatgccgg
     6421 atattgtagc acgggtgggg g
//
LOCUS       DAOQAD010000104         6386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_14934_length_6386_cov_5.913600, whole genome shotgun sequence.
ACCESSION   DAOQAD010000104 DAOQAD010000000
VERSION     DAOQAD010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14934_length_6386_cov_5.913600"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PKL60_07510"
     CDS             complement(<1..877)
                     /locus_tag="PKL60_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNW96018.1"
                     /translation="MKAPRAKTILLLLTAALALLSLHPGGVRAQAESGYDIIAEVNAL
                     RAARGLDPYTVDSWIMDYAQQHSQYQADNLNSTHRHSDGSYAWDVGLNENIAVGSAGY
                     MSPHIAVYEIWQDSVHLEPMIGYTSGAAGAGVALGADGSVYYTLNVRAGSSSTQNTPL
                     PGQTAQPAQATAPFIPLITNTPNPQGVIIHIVGPGESLWGIAVSYGVTMDDIRGLNGM
                     AAAETTIYEGQRLVIRTGVQTPLAPTPTAAGRQPDDSIPAQRHAHAHPNPHAHPFPHP
                     NRHTASSLARVLAGQP"
     gene            1059..1499
                     /locus_tag="PKL60_07515"
     CDS             1059..1499
                     /locus_tag="PKL60_07515"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNW96019.1"
                     /translation="MDQKNRVLFLCTGNSARSQMAEAFVRRYAGERVEAYSAGLEPKP
                     IHPLTYRVMDEINISLEGQYSKPVSEFLTKVHFHTLVTVCDDAEKNCPTVWPGLATRL
                     HWSFEDPAAFDGTDAEKLEKFRQVRDQIDRKVREWLELNEFRHE"
     gene            complement(1610..2587)
                     /locus_tag="PKL60_07520"
     CDS             complement(1610..2587)
                     /locus_tag="PKL60_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNW96020.1"
                     /translation="MASYLLTGAAGFIGACLADFLLREGHAVYGLDNLSPAYDLRMKH
                     YRLKSLQEKPNFHFIQDDITDKAVLERLDACMPAVDALINLAAVAGVRASMKDPWSYL
                     YTNTLGALNMLEYGARKGVKKFILASTSSLYGDEGTPPHAESAPSDHPLAPYAASKKG
                     AEAFAYAYHHLYGLDVSVLRYFTVYGPAGRPDMVMFRFCQWIAEGRPVYVTGDGEQSR
                     GFTYVDDIARGTMLALEPVGYDVFNLGGHEVITINRLIGMLEERLGRKADVRYIPTHP
                     ADVFSNVADVSKARARLGWEPQVGLEEGLSRLVEWYLRERAWAKDVETP"
     gene            complement(2587..3900)
                     /locus_tag="PKL60_07525"
     CDS             complement(2587..3900)
                     /locus_tag="PKL60_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNW96021.1"
                     /translation="MVKDEVMELINKRRANIAIIGLGYVGLPLAVTFANAGFNVTGID
                     PIQEKVDSINRGESYIMDVPNALVRKHVEAGRLRATTDYSLLAEIDAVSICVPTPLRK
                     TGDPDLSFIASASESLAPYLHRGMVIVLESSTYPGTTRELVLPKLSDCCGLKVGEDFF
                     LAFSPERVDPGRTDWTTYNTPKVVGGITPACTEVSAAWYAQALETIVPVSTAEVAEMT
                     KLLENTFRMINISMVNELAQMCDRLGIDVWEVIDAAATKPFGFMKFTPGPGMGGHCIP
                     VDPLYLSWKMKSINYTARFIDLASEIDSNMPRYVVAKIQDALNRYKKPLNGSKVLVLG
                     VAYKPNVNDLRESPAFDVIHLLQEKGAQVSYYDPYVPEVDYEFVRMNSEKDLAAAVEK
                     ADCVAIITNHSKVDYNLVFEKAQLIFDARNALKNIAKKSPKVVKL"
     gene            complement(3932..4561)
                     /locus_tag="PKL60_07530"
     CDS             complement(3932..4561)
                     /locus_tag="PKL60_07530"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNW96022.1"
                     /translation="MSAEERLQTIRAQVQRCRACPLAAGRTRAVPGEGPAGSAVMFIG
                     EGPGYHEDQQGQPFVGQAGRFLDELLGCAGLSRAEVFITNVVKCRPPNNRDPEPDELK
                     ACDEYLNAQIEAINPRVIVTLGRFSMGKFIEGGRISQIHGRPHKLDGRVVVTMYHPAA
                     ALHQPALKTTLLEDFGRLKQLLQQTETPPDLLSTPAAHKEAPPEQLSLF"
     gene            complement(4602..5123)
                     /gene="rimI"
                     /locus_tag="PKL60_07535"
     CDS             complement(4602..5123)
                     /gene="rimI"
                     /locus_tag="PKL60_07535"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNW96023.1"
                     /translation="MNPSLPIRLRRMTPADLPQVEALDRLCFRTPWPPGSFAYELRPD
                     AASICLVAECADVDGQNRIVGNVVIWLAADEAQVATLAVAPEQRGKGAARALLAAGLL
                     LAWKLGARKSVLEVRVSNEAALRLYYSLGYEAVSLRPGYYEDTHEDALLLSLAEIHPE
                     ALQKLLKPAESGF"
     gene            complement(5120..5788)
                     /gene="tsaB"
                     /locus_tag="PKL60_07540"
     CDS             complement(5120..5788)
                     /gene="tsaB"
                     /locus_tag="PKL60_07540"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNW96024.1"
                     /translation="MLLAIDTSTDWIGLALFDGTRILSEQTWRSQHYHTTELVPAIAE
                     LFARTRANKRELTGLGIALGPGSFTGLRIGMSVCKGMALALRLPVAGVPSLDILAAGQ
                     PVLRRPMIALLQVGRGRFAWARYRAANSHWSREGEVQVSDAREIAATITSPVYVCGEM
                     GEEERHILGRKWKTARLASPAQCLRRPAVLAELAWQRLLEGKADDAAALSPIYVHTLS
                     NVPE"
     gene            complement(5788..>6386)
                     /gene="tsaE"
                     /locus_tag="PKL60_07545"
     CDS             complement(5788..>6386)
                     /gene="tsaE"
                     /locus_tag="PKL60_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNW96025.1"
                     /translation="AMTNVQKPAAPEACRISPILRYNLRRLIYKKPMPILSPNTLEFF
                     SHSPEQTRRVGIRLGSLLKPGQLVCLEGDLGSGKTTLVQGIAQGWGSADPVSSPTYVI
                     INDYRHPEGTHLYHTDAYRLSGTALDIAVLDLPGMLERGVVVLEWADLIRSHLPAERL
                     WIQMQYMALEQRHLVFTPVGKAYASMCKELEKMIFGAS"
BASE COUNT         1161 a         1866 c         2051 g         1308 t
ORIGIN      
        1 caggttgccc tgccaaaacg cgggcaaggg agcttgcggt gtggcggttg gggtgtggga
       61 agggatgggc gtgcgggttg gggtgcgcgt gggcgtggcg ctgggcaggg atactgtcgt
      121 ctggctggcg gcctgcggca gtgggcgtgg gcgcgagagg ggtctggacg ccagtgcgga
      181 tgaccaggcg ctggccttcg tagatggtcg tttccgcggc agccatgccg ttcaggccgc
      241 ggatgtcatc catggtcacg ccgtacgaaa ccgcgatgcc ccacaggctt tcgcccgggc
      301 cgacgatgtg gatgatgacg ccttgggggt tgggcgtgtt ggtgatgagc gggatgaatg
      361 gcgcggtggc ctgcgcgggt tgggcggtct ggccgggcag cggggtgttt tgggtggaag
      421 agctgcccgc gcggacgttc agggtgtagt agacgctgcc atccgcgccc aaggccaccc
      481 ccgcgccggc cgcgccgctg gtgtaaccga tcataggttc caggtggact gaatcctgcc
      541 agatttcgta caccgcgata tgcgggctca tatatcccgc actgcccacc gcgatgtttt
      601 cgttcagccc gacgtcccag gcgtaactgc cgtcggagtg gcggtgcgtg ctgttcaggt
      661 tgtctgcctg atattgggaa tgctgctgag cgtaatccat aatccaggag tcgaccgtat
      721 aggggtccag accgcgggcg gcccgcagcg cgttcacttc ggcgatgatg tcgtatccgc
      781 tttccgcttg ggcgcgcacg ccaccggggt gcagagaaag gagcgctagc gctgccgtga
      841 gcagcaggag gattgttttt gcgcggggag ccttcatttt ttcaagcctc agattgtttt
      901 gattatagcc caagcgggcg ggagcaaaaa atgttggggc tggtcagacg cgctctgctc
      961 ttgatgcaaa aaggaagaca ggaaaccatc ggtgagcctt ttacaaggaa cggaaagagc
     1021 atcacgattg ggtaatattc tgagaaagag gtgaacgaat ggaccagaaa aatcgagttc
     1081 tttttttatg caccggcaac tcggcccgtt cccagatggc ggaggctttt gtccggcgct
     1141 atgcggggga gcgcgttgaa gcgtacagcg cggggttgga acccaagccc attcatccgc
     1201 tcacctaccg tgtgatggac gaaatcaaca tctcgttgga gggccaatat tccaagccgg
     1261 tcagtgaatt tctcacaaaa gtacattttc acacgcttgt gaccgtatgc gacgatgccg
     1321 agaaaaattg cccgactgtt tggccgggat tggcaacacg cctgcactgg tcatttgagg
     1381 accctgctgc ttttgacggg acggatgctg aaaagctgga aaaattccgc caggtgcgcg
     1441 atcaaattga ccgcaaggtc cgcgagtggc tggaactgaa cgaatttcgg catgaatagc
     1501 actgcgagag gacgccgcac ttctctgaat tgagccgggc gccccttcat ttcctcagat
     1561 taaagaagaa aaggctgcca atctgcagcc tggttggtct cagttcgcat cagggcgttt
     1621 ccacgtcctt agcccacgcg cgctcgcgca gataccattc caccagccgg ctcaagcctt
     1681 cctccaaccc cacctgcggc tcccagccca agcgcgcccg cgccttggaa acatccgcca
     1741 cgttgctgaa cacatccgcg gggtgcgtgg ggatgtagcg cacgtcggcc ttcctcccca
     1801 ggcgctcttc cagcatccca atcaggcggt taatcgtaat gacctcatgc ccgcccaggt
     1861 tgaaaacgtc gtaccccacc ggttcgagcg ccagcatcgt cccgcgcgcg atgtcgtcca
     1921 cgtaggtaaa tccgcgcgac tgctcgccgt cgccggtcac gtacaccggc cgtccttccg
     1981 cgatccattg gcagaagcgg aacatgacca tatccggccg tccggcgggc ccgtataccg
     2041 tgaagtagcg cagtacggac acgtccaggc cgtacagatg gtggtaagca tacgcgaaag
     2101 cctccgcgcc ctttttgctg gcggcgtacg gcgcgagcgg gtggtcgctg ggggcgcttt
     2161 cggcatgcgg gggcgtgcct tcgtctccat acaggctgct ggtggatgcc aggatgaact
     2221 ttttcacgcc tttgcgcgcg ccgtattcca gcatgttcag cgcgccgagc gtgttggtgt
     2281 acaggtagct ccaggggtct ttcatgctgg cgcgcacgcc agccactgcc gccaggttga
     2341 tcagagcgtc caccgccggc atgcaggcat ccaggcgctc cagaaccgct ttatcggtga
     2401 tgtcgtcctg tataaagtgg aagtttggct tctcttgcaa gctcttcagg cggtagtgtt
     2461 tcatgcgcag gtcgtacgcc gggctgaggt tgtccaggcc gtagacagca tgcccctccc
     2521 gcagcaggaa atctgccagg caggcgccga tgaagccggc agcgccggtg agcagatagc
     2581 tggccatcag agcttgacca ccttcgggct cttcttcgcg atgttcttga gcgcgttgcg
     2641 cgcgtcgaat atcagttggg ctttttcaaa gaccaggttg taatccacct tgctgtgatt
     2701 ggtgataatc gcgacgcagt cggctttttc caccgcggcg gccaggtctt tttcggaatt
     2761 catacgcacg aactcgtaat ccacttccgg cacgtagggg tcgtagtacg aaacctgcgc
     2821 gcccttctcc tgcagcaggt gtatcacgtc gaaagccggc gattcgcgca gatcgtttac
     2881 attcggtttg tacgctacgc ccaaaaccag tactttgctg ccgttcagag gctttttgta
     2941 gcggttgagc gcgtcttgaa tcttggcgac cacgtagcgc ggcatgttgg aatcaatttc
     3001 cgacgccagg tcgataaagc gcgcggtata gttgatggat ttcatcttcc aggagaggta
     3061 gagcgggtcc accggaatgc agtgaccgcc catccccggc cccggcgtga acttcatgaa
     3121 gccgaaaggc ttggtggcag cagcgtcaat cacttcccaa acgtcgatgc ccagccggtc
     3181 acacatctgc gccaactcgt tgaccatgga aatattaatc atgcggaagg tgttttccag
     3241 cagtttggtc atctccgcca cttccgcagt agaaaccggc acaatcgttt ccagcgcctg
     3301 ggcgtaccag gccgcgctga cttcggtaca ggccggggtg atgccgccga cgaccttggg
     3361 ggtgttgtag gtggtccagt ctgtccggcc ggggtccacg cgctcgggtg agaaggccag
     3421 gaagaagtcc tcgcccacct tgaggccgca gcaatcgctc agcttgggca gcaccagctc
     3481 gcgcgttgtg cccgggtagg tgctggattc cagcacgatg accatgccgc ggtgcaggta
     3541 cggcgcgagc gactcgctgg cgctggcgat gaaagacagg tcagggtcgc cggttttccg
     3601 caggggggtg ggcacgcaaa tggagaccgc gtcaatctcg gccagcaggc tgtagtcggt
     3661 ggtggcgcgc agccggccgg cttccacgtg cttgcgcacc agggcgttcg ggacgtccat
     3721 gatgtagctt tcgccgcggt tgatgctgtc caccttctcc tggatcgggt cgatgccggt
     3781 cacgttgaag cccgcgttgg caaaggtgac tgccagcggc agacccacgt atcccagacc
     3841 aatgatggcg atattggctc tgcgcttgtt aatcaattcc atgacttcgt ctttaaccat
     3901 atttcacctg gtttctgtgc atgttttttt tctaaaagag gcttaactgt tccggcggag
     3961 cttctttgtg cgctgccggt gtggatagga gatccggcgg cgtttcggtc tgctgcagca
     4021 gctgcttcag acggccaaaa tcttccagca gcgtggtctt cagggcgggt tggtgcagcg
     4081 ctgcggcggg atggtacatc gtgactacca cgcgtccgtc cagcttatgt gggcggccgt
     4141 gaatctggct gattctgccg ccttcgatga acttgcccat ggaaaagcgc cccagcgtca
     4201 caatcacacg cgggttgatg gcttctatct gcgcgttcag gtactcgtcg caggctttca
     4261 attcgtcagg ttccgggtcg cggttgttgg gcgggcggca tttgaccaca ttcgtaatga
     4321 acacctccgc gcgcgatagg ccggcgcacc ccagcagctc atccaggaac ctgcccgcct
     4381 gcccgacgaa aggctgccct tgctggtctt cgtggtagcc ggggccttcg ccgatgaaca
     4441 tcactgcgct ccccgcgggg ccttcccccg ggacggcgcg ggtgcggccg gcggccaggg
     4501 ggcaggcgcg gcagcgctgc acctgagcgc gaatggtttg taagcgttct tcagctgaca
     4561 ttgagtggct ctccgaaact gaattttacc ctaatacagg ttcaaaaacc gctctccgcg
     4621 ggttttaata gcttttgtaa agcttctgga tggatttccg cgagcgaaag cagcagcgcg
     4681 tcttcatgcg tgtcctcgta atagccgggg cgcaggctga ccgcttcgta gcccaagctg
     4741 tagtacaaac gcagcgcagc ctcgttgctc acgcgcactt ccaggacgga tttgcgcgcc
     4801 cccaacttcc aggccaggag cagcccggcg gccagcagtg cccgcgctgc gcctttcccg
     4861 cgctgctccg gagcgaccgc cagggtggcc acctgcgctt catccgctgc cagccaaatg
     4921 accacattcc cgacaatcct gttttgcccg tcaacgtcag cgcattcagc caccaggcag
     4981 atagaggctg catccgggcg cagttcgtaa gcgaacgaac cgggcggcca gggcgtgcgg
     5041 aagcacagcc ggtccagcgc ctccacctgg ggcaggtcag cgggggtcat ccggcgcaaa
     5101 cggatgggca gggaggggtt cattccggga cgttgctgag agtgtgcacg taaattggtg
     5161 agagcgcggc ggcgtcatcc gccttgcctt ccaggaggcg ctgccaggct aattccgcca
     5221 gcacagccgg gcggcgcaaa cactgggcgg ggctggccag gcgcgcggtc ttccatttgc
     5281 gcccgagaat gtggcgctct tcctcgccca tttctccgca cacatacacc ggggaagtga
     5341 ttgttgcggc gatttcgcgc gcgtccgaaa cctgcacttc gccctcgcgc gaccaatggc
     5401 tgttggcggc ccggtagcgc gcccaggcaa agcgcccgcg ccctacctgc agcagggcaa
     5461 tcatcgggcg gcgtaaaaca ggctgaccgg ctgccaggat atccagcgag ggcacccccg
     5521 ccacgggcag ccgcagcgcc agcgccatgc ctttgcacac gctcatcccg atgcgcaggc
     5581 cggtgaacga gcccggccca agcgcgatgc ccagaccggt cagctcgcgt ttgttcgcgc
     5641 gcgtgcgggc gaagagctcc gcaatggcgg gcaccagctc agtggtgtgg tagtgctggc
     5701 tgcgccaggt ctgttcggag aggatgcgcg tgccgtcaaa cagcgccagc ccgatccaat
     5761 cggtggaagt gtctatggct aaaagcatca ggaagctcca aaaatcattt tttccagctc
     5821 tttgcacata ctggcgtaag ctttgccgac cggggtgaag accaggtggc gctgctccag
     5881 agccatatac tgcatttgta tccacaagcg ctcggcgggc aggtgcgagc ggatcaggtc
     5941 cgcccactcg agcacgacca cgccgcgttc caacatgccg ggcaggtcca aaacggcgat
     6001 atccagggcc gtgccggaga ggcggtaggc gtcggtgtgg tagaggtgcg tgccctcggg
     6061 gtggcggtag tcgttgatga tcacgtatgt ggggctggag acggggtcgg cggagcccca
     6121 gccctgggcg atgccctgca cgagcgtggt cttgccggag ccgaggtcgc cttccaggca
     6181 gacgagctgg ccgggtttga ggaggctgcc gaggcggatg cccacgcgcc tggtttgctc
     6241 ggggctgtgg gagaagaatt cgagggtgtt agggctgaga attggcatag gtttcttata
     6301 gattaatcta cgcagattat accgcaggat gggggagatt cggcaggcct caggggcggc
     6361 aggcttttgt acgttcgtca ttgcga
//
LOCUS       DAOQAD010000105         6246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_15438_length_6246_cov_5.778226, whole genome shotgun sequence.
ACCESSION   DAOQAD010000105 DAOQAD010000000
VERSION     DAOQAD010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15438_length_6246_cov_5.778226"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PKL60_07550"
     CDS             complement(<1..579)
                     /locus_tag="PKL60_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96026.1"
                     /translation="MKKSLSLILSIGILLTLIGSLPSPAHAETQYRYASPEGSYSGDC
                     TIATPCSLQRAVDLTNGAAAGVETTIYLKSGTYVADTSRWYPEVIRITNSMNLVGRCD
                     FSSGSAVCIPENAPSTLSGETVRRIIMLQLSDPEVIHLFNLELVYGNGNEQQAGECPD
                     GGEGEARGCGGAIHALIGAYDANELKIENCVFQ"
     gene            complement(1191..3200)
                     /locus_tag="PKL60_07555"
     CDS             complement(1191..3200)
                     /locus_tag="PKL60_07555"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96027.1"
                     /translation="MKNIRRYLILFSLLAFALGTPASTQARSLTSAADTPGADFPEPQ
                     APDEVDLFPSAPERAYTAPAPAELEETSRQVNSFDCTPVTEVPAAECQALSALFGSTN
                     GAGWTTHTNWLETNTISTWYGVTVSSGHVTRLILGGNLLNGPLPTGLNALSSLQKLDL
                     HNNQLSGPIPVELANLSALQELELYGNSLSGTIPAELGSLTLLTSLTLGLNQFSGSIP
                     PELGNLTNLTRLWLYDNQLTGLIPAELGNLTKLTVLALGTNPLTGSIPPELSKLTQAW
                     SIAIEDCQLSGEIPSSFGGLTAMQYLHLDHNQLSGSIPGTLGNLANLKYLELQHNQLS
                     GSIPTTFGSLSNLSNLDLAYNRLSGSIPTQLGSLSHLTWLRLNNNELTGSIPYQLGQG
                     NFYQLDLGNNYLSGSIPVALGNLSDTLGLLLLRNNALSSAIPPELGNLTELVYLDLSG
                     NQLSGSVPASFTNLIDLCEPGDPSFPCSAGYELNLGYNRLSVPAPEPLASFLVLKDPN
                     WYQTQWQSKFIPNAFGGSLLTNDENALISVPAGAAGRDFYLEYTPLRAPGQPTTGFAY
                     AGHSFDLSAFDTAANPITSFSLPLTVWISYDEANLGGVLENELALFYWNSVSASWEDA
                     AKTCPGGIYTRNTSENWFSLPVCHFSEFALMGAGFNWLYLPVLLR"
     gene            complement(3367..4302)
                     /locus_tag="PKL60_07560"
     CDS             complement(3367..4302)
                     /locus_tag="PKL60_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNW96028.1"
                     /translation="MILQGAFIVIQQIFIIFILIGVGFLAAKFGILGEEGARQLTDLL
                     LLIVIPCVIIMAFQASFERSLIKNLLIAALLAALTHTAGILLSKLIFKNEAPDRKMML
                     AYSTVFYNAGFFSLPLLRALLGETGVLYGSVFVAVFNLFAWTYGVRMMNGGNSGSKWK
                     TFWNPGVISLLIALMLVLFNIRLPEILQTTLRYITGLHTPLAMMIIGCQFYMMRRSFN
                     LRDLGLWKTVLARNILVPLLMLALIFYLAQDKTLFFASVITASAPTATNTILFATKYR
                     QDVNTAVQTVILTTLITILSIPLMMALAMLLKGAL"
     gene            complement(5002..>6246)
                     /locus_tag="PKL60_07565"
     CDS             complement(5002..>6246)
                     /locus_tag="PKL60_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNW96029.1"
                     /translation="TTGGIMTFEQNLEKYADLIVTIGLGLKPDDKLIIYLNQDGLPLA
                     RLVSRKAYQKGVIDIRLEFTDDIMALDRYLYAPDKALEFFPQYRVEAIENLLKDGYNR
                     LSLLAEDPDLLKPVDPARIAAWQKTAGKASKPLQKYTMENKVKWCVVGVACPAWAKAV
                     FPELPAEEGMHKLWDNIFSATRADLADPVSAWKEHDAALKARKNFLNNARFDYLYYQG
                     PGTDLKVHLVQDHIWEGGSSRSQAGEVFMPNIPTEEIFSMPHADGVDGTLRATMPLSV
                     RGQIVDDFHFTFKDGRVVDFDAGTGKQILADLLEMDEGARRLGEIALVAANSPISNTG
                     ILFKNTLFDENASCHFALGAAYSENLKGATEREDAENRKLGMNDSLIHVDFMVGGRDV
                     TVTGVKPDGSQVVLLKDGEWQI"
BASE COUNT         1378 a         1608 c         1877 g         1383 t
ORIGIN      
        1 ttgaaagacg caattctcaa ttttgagctc attcgcgtca tatgcgccaa tcaatgcatg
       61 aattgctccg ccgcagccgc gagcttcccc ttccccgcca tccgggcact caccagcctg
      121 ctgctcgttg ccattgccgt agacaagctc caggttgaaa agatgaataa cctcaggatc
      181 ggaaagctgc agcatgataa tgcgccggac cgtttcaccg ctcagggttg agggcgcatt
      241 ttcggggata caaaccgcgc tgccgctgga aaaatcgcac ctgcctacca gattcatgct
      301 gttggtgatg cggatgacct ccgggtacca cctgcttgtg tcggcgacgt aggtcccgga
      361 tttgaggtag atagtcgttt ccacccccgc ggcggccccg ttggtgaggt caacggcgcg
      421 ctgcagcgag cagggagttg cgatggtgca atcccccgaa taagagccct caggagaggc
      481 gtaacggtat tgggtttcag catgcgccgg gctgggcagg gacccgataa gcgtaagcaa
      541 aatgccgatg ctaagtatca gcgacaagga ctttttcata tttacctcct gtgactaagc
      601 tgtgtaagaa ccgggctgca acgaaaaact caatcctgtg gcattcccgc tcgacgcgcg
      661 aatgtaaaga tcgtaacctt tcttgttgtt ttcctttttg ttttccgctg tcaggttcgc
      721 agtttttatt aagctctatt gtcggggagt gaattggtta accaaagtat agcacggata
      781 gccagcatat tctgcgtcaa atggtctaaa aaaattcccg gattgtgtat ttgcgacctg
      841 atgatgctct tatggcggca tgatttttcg ggcaacgctc acgcgggatt ccagctcgtg
      901 ctgttagtat gaaagccaag cggaccgaat gatcctttaa ggctcttaag ggaaatctgg
      961 caggtatgat gagacctggg ttcaatgata aattgtggta cccattaatc aaaaggaaca
     1021 ggtgggcagc cagcactcag gagactacga aaaaccactc aagaccctca tccgccttat
     1081 gggcaccttc cgccggtggg gaaggtcagg aacattcatc ctccctgcgg gaagatccgc
     1141 ttgttggggc aacggattca gagaaagcat ctccccagcc gtacggcagc ttagcgcaga
     1201 agcacaggca gatacagcca gttgaacccc gcccccatca gcgcgaactc actgaagtgg
     1261 caaacaggca gactgaacca attttcgctc gtgttgcgcg tgtaaatgcc gcccgggcag
     1321 gttttggccg cgtcctccca gctggcgctc acgctgttcc agtagaacaa agccagctcg
     1381 ttttccaaca cgccgcccag atttgcttca tcgtaggaaa tccaaactgt cagcggaagc
     1441 gagaagctcg tgatgggatt agccgctgta tcgaacgcgg acaaatcaaa gctatgcccg
     1501 gcgtaagcaa agccggtggt gggctggccc ggcgcccgca gcggggtgta ttccaggtag
     1561 aaatccctgc ctgctgcccc cgcggggacg cttatcaaag cgttttcatc attcgtgagg
     1621 agcgaacccc caaacgcgtt ggggatgaac ttggactgcc attgggtctg gtaccaattg
     1681 gggtccttga gcaccaggaa gctggccagg ggctctggcg ctggcacgct caggcggttg
     1741 taacccaggt tcaattcata gcccgcgctg caggggaaag acgggtcgcc cggctcgcac
     1801 aggtcgatga gattggtaaa gctggcaggc acgctgccgc tcaattggtt accgctgagg
     1861 tccaggtaga ccagctcagt caggttaccc agctccgggg gaatagcgct gctcagcgcg
     1921 ttgttccgca ggaggagaag ccccagcgtg tcggagaggt tgccgagcgc gaccggaatg
     1981 gagcccgaca ggtagttatt ccccaggtca agctggtaaa agttgccctg cccaagctgg
     2041 tagggaatac tgccagtcag ctcattgttg ttcaggcgca gccaggtgag gtgcgataga
     2101 ctgccaagct gggttgggat ggaaccgctg agtcggttat acgccagatc caggttggaa
     2161 agattcgaaa ggctgccaaa ggtagtcgga atggagccgc tgagctgatt atgctgaagc
     2221 tcaagatact tcagattagc gagatttccc agcgtacctg gaatggaacc gctgagctgg
     2281 ttgtggtcca gatgtaggta ctgcatcgcg gtcagcccgc caaaagagga tggaatctcg
     2341 ccgctgagct ggcagtcttc gatagcaatc gaccatgcct gggtcagttt gctcagctcg
     2401 ggggggatgg agccggtcag cgggttagtc cccagcgcca gcacggtcag tttggtcagg
     2461 ttgcccagtt ctgccgggat gaggccggtc agttggttgt cgtacagcca aaggcgggtc
     2521 aggttggtca ggttcccaag ttcaggaggg attgaaccgc taaactggtt aagtccaagc
     2581 gtgagggagg tgagcagggt caggctgccg agctcagcgg ggattgttcc gctcagggaa
     2641 ttgccataca gttccaattc ctggagagca gagaggttgg cgagttccac ggggattggc
     2701 ccgctgagtt ggttattatg gaggtcaagt ttttgcaggc tgctgagggc attcaacccg
     2761 gttggaagag gaccgttgag caggttgccg cccagtatca gcctggtcac gtgtcctgag
     2821 gaaacagtca cgccgtacca ggtgctgatg gtgtttgtct cgagccagtt tgtgtgagtc
     2881 gtccagccgg cgccgttggt actgccaaag agcgcgctga gcgcctggca ttccgcggca
     2941 gggacttcgg tgacaggtgt gcagtcgaag ctgttcacct gacgggaagt ctcttccaat
     3001 tcggctggtg caggcgcagt ataggccctt tctggtgcgg atgggaagag gtctacttca
     3061 tccggtgcct gcggttcggg gaagtccgcg cccggtgtat cagcggcgct ggtcaggctg
     3121 cgcgcctgtg tggaggcggg tgtgccaagg gcgaaggcta ataggctgaa gagaatgagg
     3181 taccggcgga tgtttttcat ttgtcgttcc tttcaacagg cgggagaagt ggtcgaaagc
     3241 agaattatac acgctggaat acagatgaag cggtaaatga acaaattctg cttttcaaac
     3301 gagatcgttg atgaaggcga ctgaattgcg cgctcggaat ggcggagggc aggaggagga
     3361 ggagatttat agcgccccct tcaacagcat ggccagcgcc atcatcagcg ggatgctgag
     3421 tatcgttatc agcgtggtca ggatgacggt ttgaaccgca gtgttcacgt cttgccggta
     3481 ctttgtggca aacagaatgg tattggtggc tgtcggcgcg gaagccgtaa ttacgctggc
     3541 gaagaacagc gttttatcct gcgccaggta aaaaatgagc gcgagcataa gcaacgggac
     3601 caggatattc cgcgccagca ccgttttcca taagcccagg tcgcgcaggt tgaaactgcg
     3661 cctcatcata tagaactggc agccgataat catcatagcc aggggtgtat gcagcccggt
     3721 gatatagcgc agcgtggtct ggaggatctc cggcaagcgg atgttgaaga gcacgagcat
     3781 gagcgcgatg aggagcgaga ttactccggg gttccagaaa gtcttccatt ttgacccgct
     3841 gttcccgccg ttcatcatgc ggacgccgta ggtccatgcg aagaggttga acacagccac
     3901 gaaaacggaa ccgtacaaaa cgcccgtttc tccaagcagc gcgcgcagca gagggagcga
     3961 aaagaaaccc gcgttgtaga aaacggtcga atacgccagc atcattttgc ggtccggggc
     4021 ttcgtttttg aagatcagtt ttgacagcaa aatgccggca gtatgagtca atgcggcaag
     4081 aagggcggct atcagcaggt ttttgatcag ggagcgctcg aaggatgcct ggaaagccat
     4141 gataattacg caggggatga cgatgagcag gagcaagtcg gtaagctggc gcgcgccttc
     4201 ttcgcccagg atcccgaatt tggcagcgag gaagccaacg ccgatgagaa tgaatatgat
     4261 gaagatttgt tggatgacga tgaatgcccc ttgcagaatc atgcgtgttc tcgcttttgt
     4321 gcttcggatg tgccgcgtgt tcgttctttt cagcgttggg ctgaaccacc cacgcgcgtg
     4381 cccgcgaatc ataacattaa ttggtcgaga atcggggaat cgcggattct atcgaggaac
     4441 agcgcaattg gaaagtatga gcgtcagcgt gagagcaagg cgaagcagcg atgcacattg
     4501 ggcacgctgt ctgccagttg aaggaacaaa ggcagaccgc cgcgccccca tcttgagagg
     4561 gttgttttca catgatttgc caacgggtgc tcgcgatgac gcgtgcttcg tcatcctgaa
     4621 tgtagtgaag gatctttctt ttccccgcca ggattctttg tctgcggctc agaatgacat
     4681 gttgcgcatt tcaacctgac aaatccaccg gtttgaaaaa cgcgagccgg cctgcgaact
     4741 gatttgctgc cgcgctcgtc ggaccatctc aaccgccagc tgcctgaccc cagcgaagtc
     4801 taaatgcaaa agaaggggct gtccaaaaag aacgggtttt ccttattagc cagctcaaag
     4861 tgggagagct tgccagttta ggccccgttt tgatagcgta aatccgtaaa cgcccataaa
     4921 aacgggcatc gagtgggaaa tcaaataggg ccgtccgact tttaggacag ccctttcagg
     4981 ttttttgcgc acaccaagcg gctaaatctg ccactccccg tctttcagca aaacgacctg
     5041 gctgccgtcc ggcttgacgc ccgtcacggt cacgtcccgc ccgccgacca tgaagtccac
     5101 gtggataagc gaatcattca tccccaactt gcggttttcg gcatcctcac gttcggtcgc
     5161 gcccttaagg ttctcactat aggcggcgcc cagggcgaaa tggcaggaag cattctcatc
     5221 gaacagggtg ttcttgaaca gaataccggt gttgctgatg ggagagttgg ctgccaccag
     5281 cgcgatttct ccgagccgcc tggcgccttc gtccatctcc agcaggtccg ccaggatttg
     5341 tttgccggta cccgcgtcaa aatctacaac cctgccgtct ttgaaggtga aatggaagtc
     5401 atccacaatc tgcccgcgca cgctgagggg catggtggcg cgcagggtgc cgtccacgcc
     5461 gtcggcgtgc ggcatgctga agatttcctc ggtggggatg ttgggcatga agacttcacc
     5521 ggcctggctg cgggaagaac cgccttccca gatatggtcc tgcaccagat ggactttcag
     5581 gtcagtgccg ggaccctggt aatacaggta gtcgaaacgg gcgttgttca ggaagttctt
     5641 gcgagctttc agggctgcgt cgtgttcctt ccaggcgctc acggggtctg ccaggtccgc
     5701 gcgggtggcg gaaaagatgt tgtcccacag tttgtgcatg ccctcctctg ccgggagctc
     5761 aggaaacacc gcttttgccc aggcggggca ggctacaccg accacacacc atttgacctt
     5821 attttccatg gtgtatttct gcaagggctt ggaggctttt ccggcggttt tttgccaggc
     5881 agcgatgcgc gccgggtcga ccggtttcag caggtccgga tcttcagcca gcagggagag
     5941 gcggttgtag ccgtccttca acaggttttc tatggcttcc acgcgatact gagggaaaaa
     6001 ctccagggcc ttatcgggag cgtacaagta gcggtccagt gccatgatgt cgtcggtaaa
     6061 ttccaggcgg atgtcgatga cacctttctg ataagctttc ctggagacca gccgcgccag
     6121 aggcagaccg tcttggttaa ggtagatgat tagcttgtca tccggtttca gtcccaagcc
     6181 gatagtgacg atcaggtcgg cgtatttttc caggttctgc tcaaaagtca ttattcctcc
     6241 ggtggt
//
LOCUS       DAOQAD010000106         6171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_15711_length_6171_cov_6.217135, whole genome shotgun sequence.
ACCESSION   DAOQAD010000106 DAOQAD010000000
VERSION     DAOQAD010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15711_length_6171_cov_6.217135"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PKL60_07570"
     CDS             <1..788
                     /locus_tag="PKL60_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNW96030.1"
                     /translation="IGVGATGPVDPCKGTILSPYTSPAWQYVPYTGLISAHFGLPCVL
                     ENDADAAALAEYWQGAGKGVKRLLAVTVGTGIGTALLIDGRIYRGMDGAHPEAGHHII
                     DPSGPLCYCGAKGCWESLASGDALTEYSRQIAAEDPQFLRELSLSAPELVTGAAVAKA
                     AERGSAKALAVMQREARYLAVGLLNLISAYVPEMIVLSGGVIENYALLRSEVEETMRK
                     NNLMVPADKVRIIPAKLGYYSGVIGGAYALLLRLQSEEKALVS"
     gene            791..1852
                     /locus_tag="PKL60_07575"
     CDS             791..1852
                     /locus_tag="PKL60_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNW96031.1"
                     /translation="MYADFPFITDILSQPEGVRNALKGYDPDSLNALSARYKAGEFDR
                     IILTGMGSSLFAAYAAWVRLAQAGLPAQWVDASELNSYAANMINSSTILWMISNSGKI
                     IEMTRLLNRREELGSPFILANTNDRDSPLGRAADLIVPIHTGNDITLATRSYMGTLAV
                     SQLMAYQLGGRPVDAAVEDLRYTADGLETYLNDLEWHIQELDQKLGEVKKLAILGRGA
                     SYATALEGALCFMEGPKLYAQGLATGQFWHGAVEMASPEFTLLMMEGEEQTSKEDEFL
                     AAKAARLGMKVFWFANESSALLPTVVLPENRGIGRTIAEIVPIQLISINVGKQTGYRP
                     GDFIHLTRTVNTKIEEFPG"
     gene            1945..2763
                     /gene="frlC"
                     /locus_tag="PKL60_07580"
     CDS             1945..2763
                     /gene="frlC"
                     /locus_tag="PKL60_07580"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: protein motif:HMM:NF007360.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HNW96032.1"
                     /translation="MKIGMFTMGYMRYPLERAFADAKRLGYDGIEIWGGRPHAYAPDL
                     KAGQLKELLGLRDKYNIEIIGYTPETNGYPYNPMIGTESMRRESIDYIKLNMDMAKAM
                     GGKFVLLSAAHAGYEATHAEIWERVIKTFTELAHYAEDIDIDIAFEPLTQYESNVIVT
                     CNDLVRALDTINSPRLVGMCDICPPFCNREPIMSYFDKLGDRLRHMHIIDSDGCSDTH
                     MMPGDGNIPLKQLFRAIEARNYQHFCTVELVMAYLNEPSLYSELAMERMRELLS"
     gene            2788..3636
                     /locus_tag="PKL60_07585"
     CDS             2788..3636
                     /locus_tag="PKL60_07585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNW96033.1"
                     /translation="MGEVIDIHTHPAFMEPICGSEELLEQRRQALGLYKTDTACLEHI
                     HNQMRCAGIDRLALLPLDLSTQENLLLVSNEEIRQLVDLDPQRFIGFASVDPYRPDAL
                     DALEHAFGDLGLAGLKLHPSRQNFSPTSDLLTPIYELCLRYNKPVIFHAGLSLEPNAS
                     MRYANPLEFEPVAARYPKLRFCLAHFGFPWVRETAALLLKYPNIYADTALLYFDSARE
                     FYEEVFTGMLGRYWIDRSLRHQVMFGSNNPRFEQLRMAEALRTLGWRSGTLDLVFGQN
                     ALEFLG"
     gene            3645..4073
                     /locus_tag="PKL60_07590"
     CDS             3645..4073
                     /locus_tag="PKL60_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNW96034.1"
                     /translation="MFFHKSNTVLTSQYNQFILITGEVAAAIKESGVRNGIVAVTSNH
                     TTAGIMVNEALECLESDIDDALQRLIPENHPYAHSRILRSYGSTAGNPTGHLKAMLTG
                     YHCLFPVVEGEILKGSAQEIYFMEFDGPSQRTYTITVQGE"
     gene            4124..5638
                     /locus_tag="PKL60_07595"
     CDS             4124..5638
                     /locus_tag="PKL60_07595"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNW96035.1"
                     /translation="MGIRYFLGVDVGTYESKGVLIDQDYNLVASKAVRHGLDNPQPNY
                     YEHDAEAVWWQDLCALTKGLIAESGIDPSQIACVGTSALGADCVPVDADCRPLRKAIL
                     YGIDSRAVGEMAFMDSYYGEEKLNALWGRPLCSSDVAPKILWIKNNEPEIYQRTHKFL
                     TATSYLTAKLTGKYVIDKFLINTFTPCYKPDGSVDEEHCQLYCRPDQLAECRGTMDIV
                     GRVTARAAAETGLAEGTPVITGTDDAGAEAISTGIFVPGDMMVMLGSSCYLIYCSDRL
                     VIDPRLWHDTFILPGAFSITGGTNNAGTVTRWFRDNIFFDKLALQEESGRNAYESILD
                     ELEGIPAGSAGLITLPYFAGERTPINDPAARGVIFGLTLQHTRGHLYKSALEGVGYSI
                     AQHLDIISEHQQPLTKIMAVGGGTQNKLWLQIIADISGHPIQTARITIGASFGDALLA
                     AVAAGNSASFAELGEKITPAEVYSPNPENQPVYARYRKLFDELYVRTKDLMRAL"
BASE COUNT         1407 a         1744 c         1754 g         1266 t
ORIGIN      
        1 gcattggcgt cggggcgacc gggccggtgg acccctgcaa aggcaccatt ctcagcccgt
       61 acacctcgcc tgcctggcag tacgtaccct acaccgggtt gatcagcgcg catttcggac
      121 tgccttgcgt gctggaaaac gacgccgacg ccgccgcgct ggcggaatac tggcaaggcg
      181 ctggcaaggg cgtcaaacgc ctgctcgcgg tgactgtggg taccggcatc gggaccgctc
      241 tcctcattga cggccggatt taccgcggga tggacggcgc gcacccggaa gccgggcatc
      301 atattatcga tccctccggc cccttgtgct actgcggcgc gaaaggctgc tgggaaagcc
      361 tggccagcgg agacgcgctg accgaatact cccgccagat tgcagccgaa gacccgcaat
      421 tcttgcggga actgagcttg agcgcgcctg agcttgtgac aggcgcggcg gtggcaaagg
      481 cggccgaaag aggcagcgcg aaagcgcttg ccgtcatgca gcgcgaagcc cgatatctgg
      541 ctgtcggttt gttgaacctg atttccgcct atgtgcctga gatgattgtg ctcagcggtg
      601 gggtcatcga aaattatgca ttgctccgct ctgaagtcga ggaaaccatg cggaagaaca
      661 atctcatggt tcccgcggat aaagtcagga ttatccctgc caaactcggg tattactccg
      721 gcgtgattgg cggggcatac gcgctcctgc tgcggctgca gagcgaagaa aaggctttgg
      781 tgagctaaag atgtacgcag attttccttt catcacggac atcctttccc aacctgaagg
      841 cgtgcgcaac gcactcaaag gctacgaccc ggacagtctg aacgcgctca gcgcccgcta
      901 caaggccggc gaattcgacc gcatcatcct gaccggcatg ggctcctctt tgtttgccgc
      961 atacgcggcc tgggtgcgat tggcgcaggc tggtttgccc gcgcagtggg tggatgccag
     1021 cgagctgaac agctacgcag cgaacatgat taattccagc actatcctgt ggatgatatc
     1081 aaattccggc aaaatcatcg aaatgacgcg cttgctgaac cggcgcgagg aactcggttc
     1141 gcctttcatt ctggcgaaca cgaatgatcg cgacagccct ttggggcgcg ctgctgacct
     1201 gattgtaccc atccacactg gcaatgacat cacgcttgcc acccgcagtt acatgggcac
     1261 gctggcggtc tcgcaattaa tggcttacca gcttggcggg aggccggtgg acgctgctgt
     1321 ggaggatttg cgttacacgg cggatggatt ggaaacctac ttgaacgatc tggaatggca
     1381 cattcaggaa ctcgaccaga agcttggcga agtgaagaag ttagccatcc tgggcagggg
     1441 cgcatcctat gccacggccc tggaaggcgc tttgtgcttc atggaagggc ctaaactgta
     1501 cgcgcaaggg ctggctaccg ggcaattctg gcacggcgcg gtggaaatgg ccagcccgga
     1561 attcaccctt ctcatgatgg aaggcgagga gcagaccagc aaggaagacg aattcctggc
     1621 tgccaaagcc gcaaggctcg ggatgaaggt gttctggttc gcgaacgaaa gctctgcttt
     1681 gctgccgacc gtggtgctgc ctgagaaccg aggcatcggt cggacaatcg cagagattgt
     1741 tcccatccaa ctgatttcca tcaacgttgg caaacaaacc ggctaccggc cgggagattt
     1801 tatccatctc acccgcacag taaacaccaa gatagaggaa tttcccgggt gaaggcgcgt
     1861 tccgcgggcg caagcgcgct gaggtaaaaa agcaacaatg cctgggtgca ttgtcatcaa
     1921 ctaacgatag aaaaggaagc tgaaatgaaa attggaatgt ttaccatggg gtatatgcgt
     1981 tatccgctgg agcgcgcttt cgcggacgcg aaacgacttg gatacgacgg catcgaaatc
     2041 tggggcggcc gcccacacgc ctacgcgcct gacctgaaag ccggccaact caaggagctg
     2101 ctcgggctgc gcgataagta caacatcgaa attattggtt acacccctga gaccaatggc
     2161 tacccgtata acccgatgat tggaacggaa tccatgcgtc gggaaagcat cgattacatc
     2221 aagctgaaca tggatatggc taaagcgatg ggcggcaagt tcgtcctgct ttctgctgct
     2281 cacgccggct atgaagctac ccacgccgaa atctgggagc gggttatcaa aaccttcacc
     2341 gagctggcgc attatgccga agacatcgac atagacatag cctttgagcc tctgacccag
     2401 tacgaatcca atgttatcgt gacctgtaat gacctggtgc gcgcgctcga taccatcaat
     2461 tccccgcgcc tggtcggcat gtgcgatatc tgcccgcctt tctgcaatcg cgaacccatc
     2521 atgagctact tcgataaact gggcgaccgc ctgcgccata tgcacatcat cgacagcgac
     2581 ggctgttccg atacccacat gatgcctggt gacggcaaca ttcctttgaa gcagcttttc
     2641 cgcgctatcg aagccaggaa ctaccagcat ttctgcactg tggaactggt aatggcctac
     2701 ctgaatgaac ccagcctgta ctctgaactg gcaatggaaa gaatgcgcga acttttgagc
     2761 tgacgctaac cagcgtggga gagatttatg ggcgaagtta tcgacatcca tacccatccg
     2821 gcttttatgg agccgatttg cgggagcgag gagctgcttg agcagcgccg ccaggctttg
     2881 ggcttgtata aaacggacac tgcgtgcctg gagcatatcc ataaccaaat gcggtgcgcc
     2941 ggcatagacc gcctcgcgct gctgccgctc gacttgagca cgcaagaaaa cctcctgctc
     3001 gtcagcaatg aggaaataag acagctggtg gaccttgacc cgcagcgctt catcggcttc
     3061 gcgtcggtgg acccctaccg cccggacgcg ctggacgcgc tcgaacacgc cttcggcgac
     3121 ctggggctgg ctggcctcaa gctgcaccct tcgcgccaga atttctctcc caccagcgac
     3181 ttactgaccc cgatttacga actttgcctg cgttacaaca aacccgtcat tttccatgcg
     3241 ggtttgagcc tggaaccgaa cgccagcatg cgctatgcca acccgttgga atttgagccc
     3301 gtcgccgcgc gctatcccaa actgcgcttc tgcctggcgc attttggatt cccctgggtg
     3361 cgggaaactg ccgcgctcct gttgaaatat cccaatatat acgcggatac tgctttgctg
     3421 tactttgaca gcgcgcggga attctacgaa gaggtattta cgggcatgct tggcaggtat
     3481 tggattgacc gctcgctgcg gcatcaggtc atgtttggct cgaacaatcc ccgctttgaa
     3541 cagctgcgca tggcggaagc gctgcgcacg cttggctggc gctcgggcac gctggacctg
     3601 gttttcgggc aaaacgcgct ggaatttctg ggttaaggag agctatgttc tttcataaaa
     3661 gcaataccgt gctcaccagt caatacaatc agttcatcct gattaccggg gaagtagccg
     3721 ccgcgataaa agagtccggc gtacggaacg gcattgtggc ggtgaccagc aaccatacca
     3781 ccgccggcat catggtgaac gaagctctgg aatgcctgga aagcgatatc gacgacgcgc
     3841 tgcagcggct tatccctgag aaccacccct acgcgcactc gcgcatcctg cgctcgtacg
     3901 gcagtactgc gggcaaccca accgggcacc tgaaagctat gttgaccggt taccattgtc
     3961 tgttcccggt ggtggagggc gaaatcctga aaggcagcgc tcaggagatt tacttcatgg
     4021 agtttgacgg accttcacaa cgcacctata ccatcactgt acagggcgag taaacaaaaa
     4081 aacataccgg acttttttta accggtaaca aggagtgagc gcaatgggaa ttcgttattt
     4141 tttgggggtg gatgttggca cgtatgaaag caagggcgtg ctgatagacc aggattacaa
     4201 cctggtggca agcaaagccg tcaggcacgg tctggacaac ccccagccga attattacga
     4261 acacgacgcc gaagctgtct ggtggcagga cttgtgcgcg ctaaccaaag ggctgattgc
     4321 cgaaagcggt attgacccat cgcaaattgc ctgcgtgggc accagcgcgt tgggcgcgga
     4381 ctgcgtcccg gttgacgcgg actgccgccc gctgcgcaaa gccatccttt acggcatcga
     4441 ttcccgagct gtgggcgaga tggcattcat ggatagctat tacggcgagg agaaactcaa
     4501 tgcgctgtgg ggccgcccgc tgtgttcctc agacgttgcc ccgaaaatcc tgtggatcaa
     4561 gaacaatgaa ccggaaatct atcagcgcac gcacaagttc ctcacggcta catcctatct
     4621 gaccgccaag ctgacgggca aatatgtcat tgacaagttc ctgatcaaca cttttacccc
     4681 ctgttataag ccggacggaa gcgtggacga ggagcactgc cagctctact gccggccgga
     4741 ccaattggcg gaatgccgcg ggacaatgga tatcgtcggg agggtgaccg cccgggcagc
     4801 cgcagaaaca ggcttggcgg aaggcacgcc ggtcattacc ggcaccgatg acgccggcgc
     4861 ggaagctatc agcaccggga tttttgtgcc cggtgacatg atggtaatgt tgggctccag
     4921 ctgctacctg atttactgct ccgaccgcct ggtcattgac ccgcgcctgt ggcacgatac
     4981 tttcattctg cccggagcat tcagcattac tggcgggacg aacaacgccg gcacggtaac
     5041 gcgctggttc cgcgacaata tcttctttga taagctggct ttacaggaag aaagcggccg
     5101 gaacgcctat gaaagcattt tggacgagct tgaaggcatc cctgcaggca gcgccggact
     5161 gattacactg ccgtactttg ccggagaacg cacacccatc aacgaccccg ccgcgcgcgg
     5221 cgttatcttt gggcttacgc tgcagcatac tcgcggacac ttgtacaagt ccgcgctgga
     5281 aggcgtcggg tacagcatcg cgcagcacct ggatatcatc agtgagcatc agcagccgct
     5341 gaccaagatt atggcggtgg gcggcggcac gcaaaacaag ctctggctgc agattattgc
     5401 cgatatcagt gggcatccga ttcaaactgc caggattacc atcggcgctt cctttgggga
     5461 cgccctgctc gcagcggtgg cagccggcaa cagcgccagc ttcgctgaat tgggtgagaa
     5521 aatcaccccg gcggaggtgt attccccgaa cccggaaaac caacccgtgt acgcgcgtta
     5581 tcgcaagctg tttgatgagc tttacgtgcg cacgaaggat ttgatgcgcg cgttgtaaag
     5641 ccgggtttgg tcaatagaaa gcagaagagc ggctggagaa aatttgtcac ttcgaggccc
     5701 tttgccagaa ggtttataag attaatatcc aggttattgg caaagggtcg tggcagtctg
     5761 cctgtccata cctccattac acagtgggct tattaatagt tcaacggtaa aaggcagatt
     5821 tccaaggtgt gcttcgctat cccacgtccg cttccagtca gtccaacact cgtgtaacct
     5881 tcccggcggg ggcttgcaac gacaaatcct tcggcaccgc gatgcaagaa acggtcaccc
     5941 gagtaggatg atccgggctc actacgggtc aacaaataag caggcagatt gccacgcccc
     6001 cttcaggtga gtctaaggtc cgtgtcatcc caacggcggg ggctcgctaa tgacaggcct
     6061 aaataccgcg ccccctactg ctgttcagac gttcgtataa tccaaacagc gggggctcac
     6121 actgacgaat ctgggtatgc cattaacata atcctcagtt cacagaaaaa c
//
LOCUS       DAOQAD010000107         6168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_15719_length_6168_cov_6.140029, whole genome shotgun sequence.
ACCESSION   DAOQAD010000107 DAOQAD010000000
VERSION     DAOQAD010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15719_length_6168_cov_6.140029"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(905..1591)
                     /locus_tag="PKL60_07600"
     CDS             complement(905..1591)
                     /locus_tag="PKL60_07600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNW96036.1"
                     /translation="MRTIQADEVIYSKRKTIAVTIFPDGKVVVRAPLHASQVVVERFL
                     RDKAGWIRSAQEQMRNAPPRAPAQRFEDGELLWYLGEQYPLELREKVPGGLAFVKGKG
                     FLLERAQKPRAEALLMAFYKRELRARVEELVRIYSARGAFRPGALRISSARTRWGSCS
                     QANVLSFSFRLALTPPACVEYVVVHELAHTREHNHSARFWQLVAQTLPEYKQPRDWLK
                     KNGALLPPIG"
     gene            1688..2272
                     /locus_tag="PKL60_07605"
     CDS             1688..2272
                     /locus_tag="PKL60_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96037.1"
                     /translation="MARPSRRFRAFPFLLLVCLLCAACTPDAGEALPLETYQDPGGRF
                     TLSIPKGWLAESAPDGSLLTLTPPDYSGTESELRILVFSALTNTVDPALHVEEATELF
                     QPFLEAYLDDTYEVINQGETKVDKAPALLFDYAKPFEESYLTGRLVMVAVPGYALAFL
                     GSAERSTWDGFLPTFRAILADFHLLSLSEGAPQP"
     gene            2269..3240
                     /locus_tag="PKL60_07610"
     CDS             2269..3240
                     /locus_tag="PKL60_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96038.1"
                     /translation="MKKRLLAVVLLPLLLLTSCNFPSPALPREDQPLYLPPAGQTLPP
                     PPTATPDPFARIKLGTEVVLPLGSLAFSPLASMDESGLPVTLSLGEDKAVMNNAEETL
                     FFSLTSESAGTNPDAAACLASLLARMQPDLPGLSASAAVDAAASGLTGLQTELSGTLL
                     GAPMTGKLAVFQVQSRCISLLGAAAAENSQALWQESGQYAFAALLGSLRVLPPGSVPA
                     CLTASDPEYAFSPEKPIRVGNVNLYDGVSRMEAYLNTLRGPNMEEISYIRLNPQFNAE
                     EEVVDAYQINYAGLSSPVILYFSIYSYETPLAPLGFSCEAAFPLRQP"
     gene            3328..4536
                     /locus_tag="PKL60_07615"
     CDS             3328..4536
                     /locus_tag="PKL60_07615"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW96039.1"
                     /translation="MPTSSRHHFTSQFWLMFAGLVLSSTGTTMIWPFMLIYASEKLGQ
                     PLTAVTSLMTINAIGALISAILAGSLADQVGRKLVMGIGLLGQAAVYLLYIPSKEFVL
                     FALLMGLSGFFVPLYRVGTDAMLADMFPPEDRAQAYALVRMGRNIGVALGPILGGLLL
                     VKSYNIGLFSAAATLAAFGLITFLFLKETRPTRTTSSAESLRDQLRVYKSALQNKFFF
                     RLIGAHTLMEICSTLVWAFLVVYLKQNFGIGEAQYSWLPTTNALMVVLLQVFVTRLTK
                     KRRSTSMLTLGAAFYTATMLIIALSSSFWGFWAAMVVMTVGELIIAPTATTFVSGIAP
                     ENQRGRYLGLFSLTWNLGMAIGPLSAGILTDQIGMRAPYFGGTLIGVLSVLAFLALDR
                     LYKKQTPAIR"
     gene            4624..5070
                     /locus_tag="PKL60_07620"
     CDS             4624..5070
                     /locus_tag="PKL60_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNW96040.1"
                     /translation="MAAEHRLTQVYVFGSRDTVQILHVAPAPGDVLFAVRHGNWEPYI
                     GAQDDHTLRWQAHQAGKIVIILPEEELPAPFSVRLTPQENRVLQLLAGGYTPAQICQM
                     MHITERSVRRYMNHLRDKFRARTLLHMLAKAVAMGMVRLNPESLED"
     gene            complement(5578..6066)
                     /locus_tag="PKL60_07625"
     CDS             complement(5578..6066)
                     /locus_tag="PKL60_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNW96041.1"
                     /translation="MKVSVDHDLCIGCGVCEGISPELFSLENTPTAEVLLSPVPEALQ
                     DAARQAAEDCPEAAILVEQDESRELFQESAERDIIVEEFADETREDVDLKLKEKKMKA
                     IVDKDLCIACGVCEGIAPEVFSLQREPYAEVLLDPIPAELEDAVEEAKEECSEGAITI
                     EA"
BASE COUNT         1248 a         1984 c         1581 g         1355 t
ORIGIN      
        1 tcgtagtcgt aggtcgggtg gagcggcagc gcaacccgac aaaacatggt cgtaggtcgg
       61 gtgaagcggc agcgcaaccc gacatgcttt ggccggcggc tttgtgcagc ccttaaaaaa
      121 agccttgaac attttctccc ggagcgggta aagcgttgtg agttcatcca ggagcagtcg
      181 taggtcgggt ggagcggcag cgcaacccga caaaacatcg tcgtatgtcg agtggagcgg
      241 cagcgtaacc cgacaagctt ggccggcggc tttgtgcagc cggcgttggg ttctgctcct
      301 gcgtcgctgc acccaaccta caaactgttc ttgcatagtc tccaaaccat aagagtttgt
      361 tgacatcctg gtctccataa ggaaattctt ctgctggccg ctactccgca cccaacgagt
      421 cgtaggtcgg gtggagcggc agcgcaaccc gacaagcttg gccggcggct ttgtgcagcc
      481 ggcgttgggt tccgctcctg cgccgctgca cccaacctac aaactgaaca gtaagatcct
      541 tcggtgccct caggatgata aacattttgt ccttgcgagc ccccgctgcc agcactacac
      601 aacaggcaga aaacccaacc agggggcgtg gcaatccgcc tgtaaatatg tgcttgcaat
      661 tctgagcgat gcgaagaacc cttacaccag taaaccgaga tccttctgtg ccctcaggac
      721 aacaaaggac ctgtacgcac ggcagagcat gccattctga gcaaagcgaa gaatttccct
      781 gtcagcaaaa ccagatcctt cggtaccctc aggatgacaa agttttactc tctaatgatg
      841 aacacccgcg ttaccggtga cagcagcccc cacgcaccgg tgtggacaac cctggctctg
      901 cccctcagcc aatcggcggc agcagcgcgc cgttcttctt cagccagtcg cgcggctgct
      961 tatactccgg cagcgtctgc gccaccagct gccaaaagcg cgcggagtgg ttgtgctcgc
     1021 gcgtgtgcgc taactcatgc accaccacgt attcaacaca tgccggcggc gtcagcgcca
     1081 accggaacga aaaactaagc acattcgcct ggctgcacga gccccagcgc gtgcgcgcgc
     1141 tgctaatccg cagcgccccg ggtctgaaag caccccgcgc ggaatagata cgcaccagct
     1201 cctccacccg cgcccgcagt tcgcgtttat agaaggccat caacagcgct tcagcgcgcg
     1261 gcttctgagc ccgctccagc aaaaagccct tacccttcac aaaagccagt ccaccgggca
     1321 ccttctcgcg cagctccagc gggtactgct cccccaggta ccacagcagt tcgccatcct
     1381 caaagcgctg cgccggcgct cgcggaggcg cgttccgcat ttgttcctgc gcggaccgaa
     1441 tccacccggc cttatcgcgc aaaaagcgct ccaccacaac ctggctggca tgtaagggtg
     1501 cccggaccac taccttccca tccgggaaaa tggtcacagc gatggttttc cgtttgctgt
     1561 aaattacctc atccgcctga atagttcgca tacccaggat tataaccaat ccccgcccgc
     1621 atccacaaaa gcccccgata cctatgcgcg cctgcgcgtt ctcctggttt tgcgatagaa
     1681 tcaacgcatg gcacgccctt cccgccgttt tcgcgccttt ccatttctcc tcctggtatg
     1741 ccttctttgc gctgcctgca cgccagacgc aggcgaagcc ctccccctcg aaacgtatca
     1801 ggacccgggc ggccgcttta ccctctccat ccccaaaggc tggctggcgg agagcgcccc
     1861 cgacggcagc ctgctcacgc tcacccctcc ggactacagc ggcaccgaat cggaattgcg
     1921 catcctggtt ttctctgccc tcaccaacac tgtggatcct gccttgcacg ttgaggaagc
     1981 aaccgaatta ttccagccct tcctggaagc gtatctcgac gacacttacg aggtcatcaa
     2041 ccagggcgaa accaaagtgg acaaagcccc tgccttgctg ttcgactacg cgaagccgtt
     2101 cgaagagagc tacctgaccg gccggctggt gatggtggcc gtgcccggtt acgcgctggc
     2161 cttcctcggc agcgctgaac gcagcacttg ggacgggttc ctgcctacct tccgcgcgat
     2221 tttagcggac ttccatttgc tttccctctc tgaaggcgcg ccccagccat gaaaaagcga
     2281 ttgcttgcgg ttgtgctgct gccactcctg ctgctcacca gctgcaattt cccctcgccg
     2341 gctctcccgc gcgaggacca gccgctctac cttccgcccg cgggtcagac gcttcccccg
     2401 ccgcccactg ccaccccgga tccgttcgca cgcatcaagt tgggcaccga agttgtgctc
     2461 ccgctgggtt cgctggcatt ttcacccttg gccagcatgg acgaaagcgg tttgcccgtg
     2521 acgctcagcc tgggggagga taaagccgtg atgaacaacg ctgaggaaac tctgttcttc
     2581 agcctcacca gcgaatccgc cggaaccaac cccgacgcgg cagcttgcct tgccagcctg
     2641 ctcgcccgca tgcagcccga cctgccgggc ctaagcgcga gcgctgctgt ggatgcggca
     2701 gcatccggcc tcaccggcct gcaaaccgaa ctaagcggca cgctcctcgg cgcgccgatg
     2761 accggaaaat tggctgtctt ccaggtccaa agccgctgca tcagcctgtt gggcgccgct
     2821 gccgcagaaa actcccaggc gctctggcag gaaagcggcc aatacgcctt tgccgcgctg
     2881 ctgggcagct tgcgcgtttt gccgcccggc tctgtgcccg cctgcctgac ggcctccgat
     2941 ccagagtacg ctttcagccc cgaaaaacct atccgcgtgg ggaatgtaaa cctgtatgac
     3001 ggcgtctccc gcatggaagc ctatttgaac accttgcgcg gtccgaacat ggaggagatt
     3061 agctatatcc ggctcaaccc gcaattcaac gctgaagaag aggtcgtaga cgcctatcaa
     3121 atcaactacg ccgggttaag ctcacccgtc atcctgtatt tttcgattta ctcctatgag
     3181 acgccgctcg cgccgctcgg tttcagctgc gaagccgcct tcccgctgcg ccaaccctga
     3241 atccgcacgc gaaaaacggg ctgcgcgcgg aatatttagt tcacttgtga taggatagag
     3301 gggttatttt acacaggtga acccttgatg ccaacttcct cccgccacca ctttacctcg
     3361 caattctggt tgatgttcgc cggtctggtg ctttcttcca ccggcaccac aatgatctgg
     3421 cctttcatgc tgatttacgc cagcgaaaaa cttggccagc cgcttactgc ggtcaccagc
     3481 ctgatgacca ttaatgcaat cggcgcgctc atctccgcca tcctggctgg gtcgctggcc
     3541 gaccaggtcg gccggaaatt agtgatgggc atcggtctgc tcgggcaagc ggcagtctac
     3601 ctgctctaca tcccttccaa agaatttgtg cttttcgcgc ttctgatggg tttatccggc
     3661 ttttttgtgc cgctttaccg ggtgggcaca gacgcgatgc tggcggatat gttcccgccc
     3721 gaagatcgcg cccaggcgta cgccctggta cgcatgggac gcaacatcgg agtggcgctc
     3781 ggcccaattc ttggcggcct gctgctggtc aagtcctata atattggcct cttctccgcg
     3841 gctgctactc tggctgcctt cggattgatt accttcctgt tcctgaaaga aacccgcccg
     3901 accagaacca catcctcggc ggaaagcctg cgcgaccagc tgcgggtcta caagagcgct
     3961 ctgcagaata agtttttctt ccggctgatt ggcgcgcaca ccctgatgga aatctgcagc
     4021 acgctggtgt gggctttcct ggtggtgtac ctcaaacaga acttcggcat aggagaggcg
     4081 cagtatagtt ggctgccaac caccaatgca ctgatggtcg tcctcttgca ggtctttgtc
     4141 accaggctca ccaaaaagcg gcgctccacc agcatgctca cgctcggagc ggccttttat
     4201 accgcgacga tgcttatcat cgcactcagc agcagcttct ggggcttctg ggcagcgatg
     4261 gtggtgatga ctgttggcga actgattatc gccccaaccg cgacaacctt tgtctcgggc
     4321 atcgcgccgg agaaccaacg cggacgctac ctggggctgt ttagtctcac ctggaacctg
     4381 ggcatggcaa tcggaccgct ctcagcaggc attctgacgg accagatcgg gatgcgcgcc
     4441 ccctatttcg gcggcacgct tatcggcgtc ctatccgtgt tggcttttct ggcgctggac
     4501 cgcctttaca aaaagcagac gcccgccatt cgctagctta gcgcgcgccc ctgcctgaat
     4561 tttccagata ttcttgcttg acaatcctct tttttccgcc gataatagaa catatattct
     4621 attatggctg ctgaacatcg ccttacacag gtttatgttt ttgggtcgcg ggataccgtg
     4681 caaatattgc atgtcgcgcc cgcacccggg gacgtcctct tcgcggtcag gcacggcaat
     4741 tgggaacctt acataggcgc gcaggacgat cacacgctcc gctggcaggc gcatcaggcg
     4801 ggcaaaatcg tgattatcct gcccgaggaa gaattgcccg caccgttttc cgttcgcctc
     4861 accccccagg aaaaccgcgt cttgcagctg ctggcaggcg gttatacccc cgcgcaaatc
     4921 tgccagatga tgcacatcac ggagcgttcc gtgcgccgct acatgaacca tctgcgggat
     4981 aaattccgcg cccgcaccct cctgcacatg ctcgcaaaag ccgtggcgat gggtatggtg
     5041 cgtcttaatc cagaaagcct ggaggactaa tcccctttaa atggaagagg ccgatagaat
     5101 cggcctctct tgcccccctc atcggtactg caaacatagc cacccttatt accggctgtc
     5161 cagaccggat tgcagttaac aaaatccgct gtctatgttt cccctatatg tacttttcat
     5221 tcagggcaac agcggaatag aactcgttca ttctggtatg aatatatcac aaaaccggca
     5281 aaatgggaaa aggaaaccgt acaaactgga taaacttcgc cgtaatatta tagttttctt
     5341 atgtcggggc ttggtccccc tcgaattaag cgcttccggg ctcattgtcg ttcagtcccg
     5401 ttccagataa tccccggtct catcccccgg catcttgaat ccattctctg gcagcgcggt
     5461 tcagatttct gccctggcga aaattcttaa gtacggcgcc cttcgccgcg cctctggcgt
     5521 tgaaagaaat aaaaacacgc cgccagtttc tggcggcgtg tcattgctct gggaagctta
     5581 tgcttcgatg gtgatggcgc cttcggagca ctcttctttg gcttcttcca ctgcgtcttc
     5641 caactcagca ggaatgggat ccagcagaac ttcagcatag ggttcacgct gcaggctgaa
     5701 aacctcagga gcgatacctt cgcaaacgcc gcaggcgatg cataaatctt tgtcaacaat
     5761 agccttcatc tttttctcct tcaattttag gtcaacatct tcgcgagtct catccgcgaa
     5821 ttcttccaca atgatatcac gctccgcgct ttcctgaaac agttcgcgcg attcatcttg
     5881 ctctaccagg attgccgctt cagggcaatc ctctgctgcc tgccgcgcag catcctggag
     5941 agcttccggg acgggcgaaa gcagcacttc ggctgtaggc gtgttttcca ggctgaaaag
     6001 ctcggggctt atcccctcgc atacgccgca gccgatacat aaatcatgat ctacacttac
     6061 tttcatacgt ttctttcact tgcagcattc tcgtgccagg cgggatagca ttcttcctgc
     6121 catccgctca gcgcttgcac cagtatatca cgacaatcct gccgcgcg
//
LOCUS       DAOQAD010000108         6058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_16161_length_6058_cov_6.916708, whole genome shotgun sequence.
ACCESSION   DAOQAD010000108 DAOQAD010000000
VERSION     DAOQAD010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16161_length_6058_cov_6.916708"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..972
                     /locus_tag="PKL60_07630"
     CDS             76..972
                     /locus_tag="PKL60_07630"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNW96042.1"
                     /translation="MKSSLRVMTLNLGGGLKNFSGTLETNASRSEALLTLIRQIKPDL
                     LCVQEIAQYVDGDGLKHSMVDLIREGAQFAHAYYGETLSMKRHMQVRKDLMVEGLFKD
                     WWDWSKGNAVFSRLPFARLGCGKLDGFPRNVPIFQPLTYEGTRDTDPRFAIIARVRQA
                     PFPYLVNLHLTTLVGERGDAPDPAVIQSARELRAEQILRVIDLVDANILKAGLPLILT
                     GDFNANPDEYALSTLLEAERGFIRLVPENPAATHPFAGEVDHFYISPAGMLREYRCQV
                     VDNKLAHRSSDHLPVVADLVFA"
     gene            997..2448
                     /locus_tag="PKL60_07635"
     CDS             997..2448
                     /locus_tag="PKL60_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96043.1"
                     /translation="MANPRYFGTDGIRGTAGTPPMSEDFVRSLGAVAARVLGGEKQSA
                     PLFVIGRDTRISGPGLQAALTEGFLASGAQVLDLGVLPTPGIAFLTRKLGADAGVVIS
                     ASHNPAPENGIKFLNAQGMKLPEEVELAIESLLGADPGNSLRAPRDGRHLREDYLQDL
                     LASQPGLDLSGKTLLLDCANGAAYEVAPELFKRLGARVLALNTAPDGLNINERAGSEH
                     LRADPSVFGSQVREAGADLGMAFDGDADRVIFVDENGRMVDGDHMLAILADFLLARGE
                     LLGETLVTTTMANGALAGYCTQKGIRLIETPVGDKYITGELLRLSLAQDAPGKLGLGG
                     EQSGHIILLDENHRTGDGIRTALFMLRVLAESGWRPLSALTGQIGKYPQLIASCNVAS
                     KPELAQLPALNALLTELRQGLPGLVRLNLRYSGTEPKCRLMLETDTSHTPAQVAAYAW
                     RVCELIQRETGTPAGAKIEVLNVSDGGLMPRPE"
     gene            2471..5017
                     /locus_tag="PKL60_07640"
     CDS             2471..5017
                     /locus_tag="PKL60_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96044.1"
                     /translation="MTRNLDFSFSADLAPRFNRLNRAVLEAEKAEEWQPAIAGMTRFL
                     WEVEDFVRRRPDLLAEDLPTSSRVLSMLLTLAATGTQGRLELYQPKDEGTRAYRRQLD
                     EDYLPSSAEMRRLAIRIARAYLSAPVFSSLSEDIRVEILPLLESLDEAQDPDRFMTYR
                     VVQIGNIYERLFALRVRTSEPLLVGTRTRAGLLREIYDRKYLRFGTSGVRGRWQNDFT
                     EKRARQVVQAICDFMNNRGVPAFVGAEDLAGKRVVIGHDTRRNSDLVTRWVAETCLAN
                     GFRVDIGNRDVPTPALAFYETDVLPPEEVAGLIICTASHNPPEWQGIKFNPRLGYPAP
                     TNVTDYIAFRINELQLEDQAGGAAELEDAEARGLVTGFDPLDQYVRWIKNNGSGNQRI
                     PIDFDRIRRFFADKHVVVDEMHGCGRGYLTRLLGETGVRHTVVHAEVDPELGGQDYAN
                     PEEPFNYLLKQTVAESGAHLGMGMDTDADRYGIVDKGGVYFRPNQILTMLVRYLGVDR
                     GLTGRVIATQTGSPLIEPLAGMIPGNAENEPAAGALPGYVGQRIYKCRVGDISSRALK
                     HAFLVPVGIKYIEEIRRMDNRYNTLKTLPENWRDRILIGGEESSGLTSRGHVTDKDGP
                     WANILIMDMLAYYGTRAENPLCTLKELWEDTVRMPGLWETFGTSSDPTSHAGRADVDA
                     PLEAKEGFINYYLDLALREDPEKLRLAGLKITYLGGIRYELVEMQLEDEHGDDHHYLR
                     VRASGTEPINRIYIESSSRETGQAMMREALRRLELITMDCLKNAHSPWHLVDMLTQTS
                     LSPDLLALVQETIRSRGWEMSDLLSKIERLSVTLEKRNRKVIGQWQQALK"
     gene            5228..>6058
                     /locus_tag="PKL60_07645"
     CDS             5228..>6058
                     /locus_tag="PKL60_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNW96045.1"
                     /translation="MKIVTDSGCDLTKEQCLELGVTMLPLKVQLGDRTYLSSVDLSAE
                     EFYELLDQTEQMPLTSTPSVGDFEDAYRKLAETDREILSIHLSSGLSSTCNAAQVAAS
                     QVDASVTVIDTLTLSSGTGWQVEAAARAVKSGWSKEQILALLKQIQAATNAIFTLPDL
                     KYLIAGGRISHLKGLLASLLGIKPVIEVNKVDGKYYDIGKKRSFLKAVQEIPALILKK
                     YGEGTALRAQIVHASNPEGGEMLREAMNKLFRCEWLADCAIGPALGAHTGRGLVGLMH
                     A"
BASE COUNT         1237 a         1806 c         1873 g         1142 t
ORIGIN      
        1 cagcgctgac gcgggctgaa aggcgaggtt aatcttggta taatcctcgc agaaagctga
       61 aaaaggaagc gactgttgaa atcatctttg cgcgttatga ccctgaacct ggggggcggc
      121 ctgaaaaact tcagcggtac gctggaaacc aacgccagcc gcagcgaagc cctgctcacg
      181 ctcatccgcc aaatcaaacc ggacctgctc tgcgtgcagg aaattgccca atatgtggac
      241 ggcgacggcc tgaaacacag catggtggac ctcatccgcg aaggcgcgca gtttgcccat
      301 gcctactatg gcgagacgct ctcaatgaaa cgccatatgc aggttcggaa agacttgatg
      361 gtggaagggc tgtttaagga ctggtgggac tggtccaaag gcaatgcggt tttttccaga
      421 ctgccattcg cgcgcctggg ctgcgggaag ctggacggct ttccgcgcaa cgttcccatt
      481 tttcagccgc tgacctacga gggcacgcgc gacaccgacc cgcgctttgc gattatcgcg
      541 cgcgtcaggc aggcgccttt tccttatctg gtcaatctgc acctgaccac actggtgggc
      601 gagcgcggag atgcgcccga ccctgccgta atccaatcag cgcgcgaact gcgcgcggaa
      661 caaattctgc gggtgataga cctggtggat gccaatatcc ttaaagccgg cctgcccctc
      721 atccttaccg gcgattttaa cgccaaccct gatgagtacg cgctttccac gctcctcgag
      781 gcggaaagag gctttatccg ccttgtgccc gaaaacccgg cagccacgca tccgttcgcg
      841 ggagaggtgg accattttta catctctccg gcgggcatgc tgcgggaata ccgctgccag
      901 gtggtcgata acaagctggc gcaccgcagt tcagaccatc tgcctgtcgt ggcggatctg
      961 gtattcgcct aactcctttg gaactggact ttttatatgg ctaaccccag gtattttgga
     1021 acagacggca tccgcggcac agcaggcacg ccgcctatgt cggaagattt tgtgcgctcg
     1081 ctcggcgcgg tagccgcgcg cgtgttgggt ggagagaagc aaagcgcgcc cctcttcgtc
     1141 atcggccgcg atacgcgcat atccggcccc ggcttgcaag ccgcgctgac cgaaggattc
     1201 ctcgcgagcg gcgcgcaggt gctggatttg ggcgtgctgc ctacccccgg cattgccttt
     1261 ctgacgcgca aactgggcgc ggacgccgga gtggtcatct cggcttcgca caaccccgcg
     1321 ccggagaacg gcatcaaatt cctgaacgcg caaggcatga aactgccgga agaggtcgag
     1381 ctcgccattg aatcgctgct gggcgcagac cccgggaaca gcctgcgcgc gccgcgggac
     1441 ggcaggcatc tgcgcgaaga ttacctgcag gacttgcttg ccagccaacc cgggctggac
     1501 ttgagcggca aaaccctgct gctggactgc gccaacggtg cggcttatga agttgccccc
     1561 gagctgttta agaggttggg cgcgcgcgtg ctcgcgttga acaccgcccc cgatggtttg
     1621 aatatcaacg agcgcgccgg ctcggaacat ctgcgcgcgg acccctccgt atttggcagc
     1681 caggtacgcg aagctggcgc cgacctgggt atggcttttg acggcgatgc cgaccgcgta
     1741 attttcgtgg atgaaaacgg caggatggtg gacggcgacc atatgctggc cattctggcg
     1801 gatttcctcc tggcgcgcgg cgagcttttg ggggaaacgc tggtaaccac cacgatggcc
     1861 aacggcgccc tggcaggtta ctgtacgcaa aaaggcatcc ggcttatcga aaccccggtg
     1921 ggcgacaaat atatcaccgg ggagctgctg cgcctgagcc tcgcgcaaga cgcgccggga
     1981 aagctgggcc tgggcggaga gcagtccggg catatcatcc tgctggacga gaaccaccgc
     2041 accggcgacg gcatccgcac cgcgttgttc atgctgcgcg tgctggcaga aagcggctgg
     2101 cggccgctct ccgcgctcac cggccaaatc ggcaagtatc cgcagttgat tgcttcctgc
     2161 aatgtggcca gcaagcctga actggcgcag ctgccagcgc tcaacgcgct gctcacggag
     2221 ctccggcagg ggctgcccgg cctggttcgc ctgaatttgc gctactccgg aacggagccg
     2281 aaatgcaggt tgatgctcga gaccgacact tcccacactc ccgcccaggt ggcggcttat
     2341 gcctggcggg tgtgcgagct catccagcgc gagacgggca ctcccgccgg cgcgaaaatt
     2401 gaagtattga atgtttcgga cggcggtttg atgccgcggc cggagtgaaa ttcccaatga
     2461 ggagaaatgt atgacccgga acctggattt cagcttcagc gcggatttgg cgccgcgctt
     2521 taaccgcctc aaccgcgccg tcttggaggc agaaaaagcg gaagaatggc agccggctat
     2581 cgccgggatg acccgctttt tgtgggaagt ggaagatttt gtgcggcgcc gccccgatct
     2641 gctcgcggaa gacctgccga cctcctcgcg cgtgctcagc atgctcttga ccctggcggc
     2701 tactggcacg caggggcgcc tggaacttta ccagcctaag gacgaaggaa cccgcgccta
     2761 ccggcggcag ctggatgagg attacctgcc ttccagcgcg gaaatgcgcc ggcttgccat
     2821 ccgcattgcc agggcgtacc tgagcgcgcc ggtattttcc agcctgagcg aggatatccg
     2881 cgtggaaatc ctgccgctgc tcgagagcct ggatgaggcg caggacccgg accgctttat
     2941 gacctaccgc gtcgtccaga ttggcaatat ctatgagcgc ttattcgcct tgcgggtgcg
     3001 taccagcgag cccttgcttg tgggtacgcg cacgcgcgcc ggcttgctgc gggaaatata
     3061 cgaccgcaaa tacctgcgtt ttggtacctc gggcgtgcgt gggcgctggc agaatgactt
     3121 caccgaaaag cgcgctcgcc aggtggtgca ggctatctgt gattttatga ataaccgcgg
     3181 cgtacccgcc tttgtgggcg cggaagacct ggcgggcaaa cgggtggtca tcgggcacga
     3241 tacccgccgt aattccgacc tcgttacgcg ctgggtggcg gaaacctgcc tggccaacgg
     3301 gttcagggtc gacattggca atcgcgatgt gcccaccccg gcgctggcat tctacgagac
     3361 ggatgtgctg ccgcctgaag aggtggccgg cctgattatc tgcaccgcca gccacaatcc
     3421 gcccgagtgg cagggcatca aattcaaccc ccgcctgggt tatccggcgc ccaccaacgt
     3481 gacggattac atcgccttcc gtatcaacga actgcagctg gaagaccagg ccggcggcgc
     3541 ggcggagctg gaagacgcgg aagcgcgcgg acttgtgacc ggctttgacc cgctggacca
     3601 gtatgtgcgc tggattaaga acaacggcag cggcaatcag cgcatcccga ttgattttga
     3661 tcgcatccgg cgcttctttg ccgataagca cgtggtcgtg gatgaaatgc acggctgcgg
     3721 gcgcggctat ctgacgcgcc tgttgggcga aaccggcgtg cgccacaccg tcgtgcatgc
     3781 ggaagtggac cccgagttgg gtgggcagga ctatgccaac ccggaagagc ccttcaacta
     3841 cctacttaag cagaccgtgg cagaaagcgg tgcgcacctg ggtatgggca tggatacgga
     3901 cgccgaccgc tacggtatcg tggataaggg cggggtgtac tttcggccaa accaaatcct
     3961 gaccatgctg gtacgctacc tgggcgtgga ccgcgggctg accgggcgcg taatcgccac
     4021 gcagactggc tcacccttga ttgagccgct ggcaggcatg attcccggga atgccgaaaa
     4081 cgaacctgcc gcgggcgcgc tgcccggcta cgtgggtcag cgcatctaca agtgccgcgt
     4141 gggcgatatc tccagccgcg cgctgaagca tgcttttttg gtgccggtcg gcatcaaata
     4201 tatcgaagaa atccggcgca tggataaccg ctacaacacc cttaagacgc tgcctgagaa
     4261 ctggcgcgac cgcatcctga ttggcggcga ggaatcctcg ggcttgacct cgcgcgggca
     4321 cgtaaccgac aaggacggcc cctgggcgaa tatcctgatt atggacatgc tggcgtacta
     4381 cggcacccgc gcggaaaacc cgctgtgcac cctcaaagaa ctctgggagg ataccgtgcg
     4441 gatgcccggc ctgtgggaga ctttcgggac ttcctccgac ccgacttcgc acgcgggcag
     4501 ggccgacgtg gacgcgccgc tggaggctaa agagggcttc atcaactatt acctcgacct
     4561 ggcgctgcgc gaggaccctg aaaagctgcg cctggcaggc ttgaaaatca cgtacctggg
     4621 cggcatccgc tacgagctgg tggaaatgca gctggaggat gagcacggcg atgaccacca
     4681 ctacttgcgc gtgcgcgcct cggggacgga accgattaac cgcatctaca tcgagtccag
     4741 cagccgggaa accgggcagg ccatgatgcg cgaagccctg cggcggcttg agctgattac
     4801 gatggactgc ctgaaaaacg cgcattcgcc ctggcatctg gtggatatgc tcacccagac
     4861 ctcgctgagc cctgacctgc tggcgctcgt acaggaaacc atccgcagcc gcggctggga
     4921 gatgagcgat ctgctttcga aaattgagcg tctgagcgtt accctggaaa aacgcaaccg
     4981 caaggtcatc ggtcagtggc agcaagcgct taaatgagtt tgcgacacgc gagccacagc
     5041 cccccgggaa cgggggctgc ggccattttc gccggcctta tttgttatcg ttgcgtgcta
     5101 taatttagag accacaaacc cattacgatt tccgtcaacc caaatggcag aatccccgac
     5161 acttacggcg gcgacgatgc tcgcggatag gcgaattttg catacgactt gaaaacgagg
     5221 aaaagaaatg aaaattgtta cagacagcgg ctgcgacctg accaaagagc agtgcctgga
     5281 attaggggtt acgatgcttc ctttgaaggt tcaacttggc gaccgcactt acctcagcag
     5341 cgtggatttg agcgctgagg agttctacga actgttggat cagactgagc aaatgccgct
     5401 cacatccacg ccttctgtgg gagactttga ggatgcttac cggaaactgg ctgagactga
     5461 ccgcgaaata ctatcgattc acctttcttc cggattgagc agcacctgca atgccgcaca
     5521 agtagctgcc agtcaggtgg atgcttccgt gactgtcatc gacacgctca cactctcctc
     5581 cgggacgggc tggcaggtgg aagctgcggc gcgcgcggtg aaatccggct ggagcaagga
     5641 gcaaatcctg gcgctgctca agcagattca agccgcgacc aacgctatat tcaccttgcc
     5701 tgaccttaag tacctgattg ccggcggacg catcagccat ttgaaaggcc tgctggcttc
     5761 cctgctcggc atcaaaccgg ttatcgaggt caataaggtt gacggcaagt actacgacat
     5821 cggcaagaag cgctctttcc tgaaggctgt gcaggaaatc ccggcgttaa ttctgaaaaa
     5881 gtacggcgaa ggcaccgcgt tgcgcgccca gatcgtgcac gcctccaacc cggaaggcgg
     5941 cgagatgctg cgcgaagcca tgaacaagct gttccgctgc gaatggctgg cggactgcgc
     6001 gattggcccg gctctgggcg cgcataccgg ccgaggcctg gttgggctga tgcacgcg
//
LOCUS       DAOQAD010000109         6010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_16331_length_6010_cov_5.687154, whole genome shotgun sequence.
ACCESSION   DAOQAD010000109 DAOQAD010000000
VERSION     DAOQAD010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16331_length_6010_cov_5.687154"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..2154)
                     /locus_tag="PKL60_07650"
     CDS             complement(274..2154)
                     /locus_tag="PKL60_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW96046.1"
                     /translation="MKGDHKPTPYPSGSKQQTENANGHFSIKNFFKALRNASNAFKLV
                     WAASKPNAVFNFGLTPIAALMPAGQAWVGKLIVDKILGVVNAGTGWQAGLREVLPVVL
                     LELVLVLVSAVVSQARLLSNRMMSLQLTTHVNTLIIQKAISLDLQFFEDPVFYDVLQN
                     ARRQSDSAALSIVTSTMQIAQQTITLVSLIALLVRFSPFLALIVFAAAIPSFLSDTQY
                     AEMSYRMIYKRAPEMRLLSYLEMLLTGSETFKEIKLFGLGQPLLERFKALFIRFFNED
                     MTVARKRTLAGLAWGVLSSLVYYGSYVWVIYRTIIQAVTLGDMTMFLSIFRQSQNSIS
                     SLLDNFNSIYENNLYLDNLLNYLKITPALSNPAEGVAAPVTIKEGIEFRHVSFKYPGS
                     DKFVLKDVNLFIPPGESIALVGLNGAGKTTLVKLLTRLYDPTEGAILLDGRDLREYDL
                     NSLYQRFGVIFQDYARYQFSVRENIGFGQIDEVDNLEKVREAAERGGADKVVEELPDG
                     YETILGRRWEKGLELSGGQWQKIALARAFMRDAEVMILDEPTSALDAEAEYEIYKRFR
                     ELMHGRIAVLISHRFSTVRMADRIVVLSEGRVLEVGSHAELMQSGGPYAHLFNLQAEG
                     YR"
     gene            2288..2896
                     /locus_tag="PKL60_07655"
     CDS             2288..2896
                     /locus_tag="PKL60_07655"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNW96047.1"
                     /translation="MNWIALALAAFLCGAIPFSVWLSWLFYRLDPRTYGDGNPGAMNT
                     FHTGKAWLGILVLLLDVSKAVLPVGYAYRALGVRGIPMAVIAIAPMLGHVFSPFLRFR
                     GGKALACALGVWIGLGGWTIAGAAVLGALLGKALFTDDGWSGLTGLVLVLPAIFIFRA
                     DKLLLLVWLLQAVLLAWTLRGALAKRPQLKPWLKRLFMGSKP"
     gene            2898..4061
                     /locus_tag="PKL60_07660"
     CDS             2898..4061
                     /locus_tag="PKL60_07660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW96048.1"
                     /translation="MIDSYFSRDLIVHFILFQGILLVVLLSNLYLLRKARRGKAPDAA
                     PFVSILVPARNEEANIARLVESLLAQDYPDYEILVLDDNSSDATPKILRALTEANPKL
                     TVLSGEPDPDGLTGKNRACAQLASRAAGELLFFTDADTVHQPGALRELVACLQRENAD
                     FLTGFPKQELGTWGERLLVPFFSWAVLNFISLGLAYCLRWKALAVGIGQVMLFRREAY
                     EQIGGHAALGEEIVDDRALARNIIAAGRRWRAVHLSDLVSCRMYTNAKAALEGFTKNY
                     FAVFDFAVLPYVFVYLYLLLMAWLPLVVLLAKVFGAAPDANFAHLALCVGLSVLLWLL
                     PFLEARIPAGLALLYPLLAAASTLTAFRSLVMSLSGRLTWKGRPLAKQRWKWF"
     gene            complement(4106..5551)
                     /locus_tag="PKL60_07665"
     CDS             complement(4106..5551)
                     /locus_tag="PKL60_07665"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNW96049.1"
                     /translation="MNIRLSTGKLLPIEIHKIKIVQETSLPPIRQRQEAIQTAGFNTF
                     LLRSRDIFLDMLTDSGTNAMSDRQLAAMMMADDAYAGSESFYRLEESAREVLGFQFVM
                     PVHQGRAAEHLLAKAFVQPGSVVLMNYHFTTSKAHVDLAGGKVLELCADEALNTSSSN
                     QFKGNMDLGKLEAAIREWGRERVAYVRMEASTNLIGGQPFSMQNLKDVKALASRHGIP
                     LVFDGSLISENAYFIQQREPEFADWSVQEIILEMMRSVDIFYMSGRKSAAVRGGLIAT
                     NSKEVFDKLLVWLPVYEGFATYGGMSTKEVEAMAVGLREMTAQSVASSAADFIAYFVE
                     RLLELGVPVVTPSGGLACHVDARRFIPQVPQSLYPAGALAAAVYLVSGVRGMERGSVS
                     MDRDAQGRDVPADMELLRLALPRRTFTLSHIEYTADRLKWLFAHRELIGGLRFSEEPP
                     VLRFFFGRLQPVDGWVGNLVDAFRAEFGEGC"
BASE COUNT         1191 a         1844 c         1700 g         1275 t
ORIGIN      
        1 acctacgacc acgtcccgtc gggttgcgct ggcgctccac ccaacctacg accacgtccc
       61 gtcgggttgc gctgccgctc cacctgacct acgacctgcc gctccaccca acctacgact
      121 gatgcatcct taagaagcat cgcggatgac aatccacaag catcttggga tgcctcgccc
      181 atccttctaa cgaaggtacg gttaccaaaa aaaccaggcg catatcccca aacttctggt
      241 cggcggctgt gcccacctaa gcaaattcag gatttaccgg tatccttccg cctggaggtt
      301 aaacaggtgc gcgtaaggtc cgccgctctg catcagctcc gcgtggctgc ccacctccag
      361 aacccgccct tccgaaagta ccacaatccg gtccgccata cgcacggtgg aaaaccgatg
      421 cgaaatgagc acggcaatcc gtccgtgcat cagttcacgg aaacgcttgt agatctcata
      481 ctccgcttcc gcgtccaggg cggaggttgg ttcgtccaga atcatcactt ctgcgtcgcg
      541 cataaacgca cgcgccaggg caatcttctg ccactgcccg ccggagagct ccaagccctt
      601 ttcccagcgc cggcccagga tggtttcata cccgtcgggc agctcctcga ccaccttatc
      661 cgcgccgccc cgttccgctg cctcgcgcac tttttcgagg ttatccacct catcaatctg
      721 cccgaaacca atgttctcgc gcacgctgaa ctgataccgc gcgtaatcct ggaagatgac
      781 tccaaagcgt tgatacaaac tgttcaggtc gtactcgcgc aggtcgcgcc cgtccaaaag
      841 gatcgcgcct tccgttggat cgtacagccg ggtcagcagc ttcaccagcg tggttttgcc
      901 ggcgccgttc aaacccacca acgcgatgct ttcccccggc ggaatgaaca ggttgacatc
      961 ctttagcaca aacttatctg agcccggata cttaaaggaa acgtggcgga actcaatgcc
     1021 ttccttgatt gtcaccggag ccgcaacccc ttcggcgggg ttggaaagcg cgggggtgat
     1081 tttcaggtag ttcagcaggt tatccagata cagattgttc tcgtagatac tgttaaagtt
     1141 gtccagcagg gaactgatgg agttttgcga ctgccggaag atagacaaaa acatggtcat
     1201 atctcccaag gtgaccgctt gaataatcgt ccggtagata acccacacat agctgccgta
     1261 ataaaccagg ctggaaagca cgccccaagc caggcctgcc aacgtgcgtt tccgggcgac
     1321 cgtcatatcc tcgttgaaaa agcggatgaa gagggcctta aagcgctcga gcagcggctg
     1381 ccccagcccg aaaagcttga tttccttgaa agtctcgctg cccgtcagca gcatttccag
     1441 gtacgagagc agcctcatct ccggcgcgcg cttgtaaatc atgcggtaag acatctcggc
     1501 gtactgcgta tccgaaagga agctggggat agccgccgcg aagacaatca acgccaggaa
     1561 cgggctgaag cgcaccagca aagcaatcaa ggaaacgagt gtgattgtct gctgcgcgat
     1621 ttgcatggtc gaggtaacaa tcgagagcgc cgcgctgtcg gattgacggc gggcgttctg
     1681 caggacgtca tagaaaaccg ggtcctcgaa aaattgcagg tccaggctga ttgctttctg
     1741 gataatcagg gtgttgacat gcgtggtcag ctgcaggctc atcatccggt tggaaagcag
     1801 gcgcgcctgg ctcaccacgg cgctgaccag cacgagcaca agctccagca gcaccaccgg
     1861 aagcacttcc cgcaggccgg cttgccatcc tgtgcctgcg tttaccacac caagaatctt
     1921 atcgacgatg agtttgccaa cccaggcttg ccccgcgggc atcagcgccg cgatgggcgt
     1981 caaaccgaaa ttgaacacag cgtttggttt gctggcagcc caaactaatt tgaacgcgtt
     2041 ggacgcattg cgtagcgctt tgaagaagtt tttgatgctg aaatgcccgt tggcgttctc
     2101 tgtctgctgc ttgctgccgg aggggtaggg tgttggtttg tggtcgcctt tcatgttttc
     2161 tccctgtcgc tgcattactt gcccgaacag atggtcatta taccgccttg aacggactgc
     2221 ccgccgttct tgcgttcaag cgcattcccg tgaataaacc gacctgattt cggatacaat
     2281 tttccctatg aactggatag cgcttgctct ggcggctttc ttgtgcgggg ccatcccttt
     2341 ctcggtgtgg ctcagctggc ttttctaccg cctggacccg cgcacctacg gggacggaaa
     2401 cccgggcgcg atgaacacct tccacacggg caaggcctgg ctggggattc tggtcctgct
     2461 gctggatgtc agcaaagctg tgctgccagt cggatatgcc tatcgcgcgc tcggcgttcg
     2521 cggcatcccc atggccgtaa tcgccatcgc gcctatgctc gggcacgttt tcagcccttt
     2581 cctgcgcttt cgcggcggca aagccctggc atgcgcgctg ggggtttgga ttggtttggg
     2641 cggctggact atcgcgggcg cggcggtgtt gggggcgctg ctggggaagg ctctcttcac
     2701 agatgacggc tggtccggct tgaccggcct ggttttggtc ctgccagcca tctttatctt
     2761 ccgggcggat aagctgctgc ttttggtttg gctgctgcaa gctgttctgc tggcctggac
     2821 cctgcgcggg gctctggcta aaagaccgca gctcaagccc tggctcaaac ggcttttcat
     2881 gggctccaaa ccgtaagatg atagacagct acttctcccg ggacctcatc gtgcatttca
     2941 tcctttttca gggcatcctg ttggtggtgc tgctcagcaa cctctatctg ctgcgcaaag
     3001 cccggcgggg gaaagcgcca gacgcggcgc ctttcgtttc catcctggtg cctgcccgca
     3061 acgaagaagc caatatcgcc cggctggtag aatccttgct cgcgcaggac tatccggatt
     3121 atgagatcct cgttctggac gacaattcca gcgatgccac ccccaaaatc ctgcgggcgc
     3181 ttacggaagc caacccgaag ctgaccgtcc tttcgggcga gcctgacccg gacggcctca
     3241 ccggtaagaa ccgggcttgc gcgcagcttg ccagccgggc agcgggcgaa ctcctgttct
     3301 ttaccgacgc ggacaccgtc catcagcctg gggcactgcg ggaattggtg gcgtgcctgc
     3361 agcgcgaaaa tgccgacttc ctgacaggct tcccgaaaca ggaactcgga acctgggggg
     3421 aacgtctgct ggtacccttc ttctcctggg ctgtgctgaa tttcatctcg ctcgggctgg
     3481 cgtactgcct gcgctggaaa gccctggcag tcggcattgg tcaggtaatg ctcttccgac
     3541 gcgaagccta cgagcagatt ggcgggcatg ccgcgttggg tgaggaaatt gtggatgacc
     3601 gggcgcttgc gcggaacatc atcgccgcgg gcaggcgctg gcgcgccgta cacctttcgg
     3661 acctggtcag ctgccggatg tataccaacg cgaaagccgc cctggaaggg ttcaccaaga
     3721 actacttcgc ggtctttgac tttgccgtgc tgccctatgt gttcgtctac ctatacctgc
     3781 tgctgatggc ctggcttccg ctggtcgtct tgctcgcgaa agttttcggc gcggcgccgg
     3841 acgcgaactt cgcgcatctg gcgctgtgcg tgggtctatc cgtgctgctc tggctgcttc
     3901 cattcctgga ggcgcgcatc cccgcggggc tcgcgctgct ctaccccttg ctagccgctg
     3961 ccagcacgct gaccgctttc cgctcgctgg taatgagcct gagcgggcgc ctgacctgga
     4021 aaggacgccc gcttgccaaa cagcgctgga aatggtttta ggttttgttt cgaatcggaa
     4081 cacacccggt tcgtggttga tgaaactaac agccctcccc aaactccgcg cggaaagcat
     4141 ccactagatt tcccacccaa ccgtccaccg gctgcagcct gccaaagaag aagcgcagca
     4201 caggcggctc ctcgctgaag cgcagcccgc caatcaactc gcggtgcgcg aagagccatt
     4261 tcagtcggtc agccgtgtat tcaatatgcg aaagcgtgaa ggtgcgccgc gggagcgcca
     4321 gcctcagcag ctccatatcc gccggcacat cgcggccctg cgcatcccgg tccatcgaaa
     4381 cgctgccgcg ctccattccg cgcacgcccg aaacgaggta tactgccgcg gctagcgcgc
     4441 cggccgggta caaagactgc ggcacctgcg ggatgaagcg ccgcgcgtcc acgtggcaag
     4501 ccagcccgcc ggagggcgtc accactggca cgcccaactc cagcagccgc tccacaaagt
     4561 aggcgataaa atccgcggcg ctgctggcca ccgactgagc ggtcatttcc cgcaggccga
     4621 ccgccatcgc ttccacctcc ttcgtggaca ttcccccgta ggtggcaaag ccctcatata
     4681 ccggcagcca gaccagcagc ttgtcgaaaa cctccttgga gttggtcgca atcagaccgc
     4741 cgcgcaccgc ggcgcttttg cgcccggaca tgtagaagat atccacgctg cgcatcattt
     4801 cgagaataat ttcctgcacg ctccaatcgg caaactcggg ttcgcgctgc tgaataaagt
     4861 aggcgttttc agaaatcagg ctgccgtcga acaccagcgg gattccgtgc cgagaagcga
     4921 gcgctttgac gtctttcagg ttttgcatcg agaagggctg gccgccaatc aggttggtgg
     4981 aggcttccat gcgcacgtac gccacgcgct cgcggcccca ctcgcggatg gcggcttcca
     5041 acttgcccag gtccatgttg cccttgaatt gattgctgga gcttgtgttc agcgcttcat
     5101 ccgcgcaaag ctccagcacc ttgccgcctg ccaaatccac atgcgctttg gaggtggtga
     5161 agtggtaatt catcaacacc acgctgcccg gctgcacgaa ggccttagcc agcaagtgct
     5221 ccgcggcgcg gccctggtgc accggcatca caaactgaaa gcccagcacc tcacgggcgg
     5281 attcttccag gcggtaaaaa ctctcgctgc ccgcgtaggc atcgtccgcc atcatcatcg
     5341 ccgccagctg gcggtcgctc atcgcgttcg tgccgctgtc ggtcagcata tccagaaaga
     5401 tatcgcgcga gcgcagcagg aaagtgttga aaccggcggt ctggatggct tcctgacgct
     5461 gccggatggg cggcaggctg gtctcctgaa cgatttttat tttgtgtatt tcaatgggca
     5521 gtaattttcc ggtagataaa cgaatattca tgttcttttc cttatcagtt tgggggatta
     5581 ataacggaaa gggaacccgc cgcctgggca ggtttggaag gataaaaaag ggacaccccc
     5641 ggcgttaacc agccggaaat gcccctaaac gtaataatag gtatggatga ttttcttcag
     5701 ggaaagcgtt gaggggttaa taaaaactca ccatgcgccc gattttatca taaagacagg
     5761 aaaagcaagc ctatttggca gaattcgccg ggggttggca gaattcgccg ggggttaaag
     5821 ttggcgggct gggcggaagg gcgagggcgt tgcccgcggc accttttcca ggtaatggtc
     5881 ccagaatcgg gctgcggtcc gggtcacacg gcagaaaata atccggtctg ctaccgttct
     5941 catccgcctc ttgtgccgat gcccgcggct ccggttgcca ggctgtagtt attccgccgc
     6001 cgctattcgc
//
LOCUS       DAOQAD010000110         5964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_16521_length_5964_cov_5.455069, whole genome shotgun sequence.
ACCESSION   DAOQAD010000110 DAOQAD010000000
VERSION     DAOQAD010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16521_length_5964_cov_5.455069"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1081
                     /gene="rpsB"
                     /locus_tag="PKL60_07670"
     CDS             224..1081
                     /gene="rpsB"
                     /locus_tag="PKL60_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNW96050.1"
                     /translation="MSPAISMKALLESGVHFGHRTNKWNPKMRPYIFTERNGIHIIDL
                     QQTVKLLDEAFNVVRDAVAAGGTILFVGTKRQAQETIQEEAERCGMPYVNQRWLGGTL
                     TNWVTIQQRIVELTRLEKLVESGEINQLTKKEGLLIQREIARLETRLSGVRNMKRLPN
                     LVFIVDIEREETAMKEAYIKEIPIIAMVDTNCNPSMIDYVIPSNDDAIRAIKLIVGRI
                     ADAAEEGRMMRKEEEVEEVQPESRSVISRKIDEDIELGDDELLGDATRAKIPAKEEPE
                     TGETPAVEA"
     gene            1139..1759
                     /gene="tsf"
                     /locus_tag="PKL60_07675"
     CDS             1139..1759
                     /gene="tsf"
                     /locus_tag="PKL60_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNW96051.1"
                     /translation="MQITIDMIKELREKTGCGIMDCRSALEKSNGNMEQAMMELREKG
                     LKTAEKRADRVASEGRLEVYVHSDGRLVVMVELNCETDFVAKTASFQELAHEIALQIA
                     AAQPTYISEADIPAEVIEAETKAVAERVRAEGKPEAIIPKIVDGYMKKFKDEKVLLNQ
                     KYIRDESKTVADLITDKVSSLGENLIVRRFVRWALGETTQPQESAE"
     gene            1815..2546
                     /gene="pyrH"
                     /locus_tag="PKL60_07680"
     CDS             1815..2546
                     /gene="pyrH"
                     /locus_tag="PKL60_07680"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNW96052.1"
                     /translation="MTEQGTAPKYKRILLKLSGESLAGPSGFGIDPKEAAHIANLVKR
                     VHALGVDVAIVIGAGNLWRGATGENSGMDRATADYMGMLATVINALALMDALERVEVI
                     TRVQSAIEMPTVAEPYIRRRAIRHLEKGRVVIFGGGTGNPFFSTDTAAALRATEIGAD
                     VVIKATKVDGVYDSDPKTHPEAVKFDQLSYMETVSRRLSVMDTTAVTLCMENDIPILV
                     LNLWEPEALIRGILGESVGTLVSCD"
     assembly_gap    2702..2801
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3196..4413)
                     /gene="gguB"
                     /locus_tag="PKL60_07685"
     CDS             complement(3196..4413)
                     /gene="gguB"
                     /locus_tag="PKL60_07685"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNW96053.1"
                     /translation="MSENPNIQEERAPSFLAEMVGLLKSNISQYFMFIALIVIMIFFT
                     ITTDGVFISSRNISNLINQMGYIAVLAVGMTLVIVIRHIDLSVGYLAGFLGAFAAIAM
                     TAWGLPVYAVIPVVLILGIASGLLTSTPIAKLGVPAFVSTMAAGLIFRGALLLVTMGT
                     GTIIIKDKVFNAIGNGFIPDIPDFGFLDNNHKVTLLVGALGIILYILSAIKGRKTKLS
                     YNFKVPSVPMFIVELVLISAIIGYVSWVLSGYNGLSWSLVVVLLVVAVFTFITTKTPL
                     GRHIYAVGGNPEAAELSGISVSKITYIVFGAMGMLASLSGILYASRLQSATTTAGLGF
                     ETDAIAAAFVGGVSAAGGVGTIPGSIIGALVMMSLTSGMNLMGVDISIQYIVRGAVLV
                     GAVIVDRATRKGK"
     gene            complement(4425..>5964)
                     /gene="gguA"
                     /locus_tag="PKL60_07690"
     CDS             complement(4425..>5964)
                     /gene="gguA"
                     /locus_tag="PKL60_07690"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNW96054.1"
                     /translation="KQEKLMEDSILEMKSITKEFPGVKALDGVSFSVARGEIHCLVGE
                     NGAGKSTLMKVLSGVHPFGSYSGEIFIDGELQKFNGIRDSEKAGIAIIYQELALVPEL
                     SVYENIFLGNEIRNGFIIDTNETIKEADEILKKVGLDVSPEVKVKTLGVGKQQLIEIA
                     KALKKEVKILILDEPTAALNETDSDNLLELIKGLKQQGVTCILISHKLREVLSIADTI
                     TVLRDGKTICTLDNKEHQVTDRVLIKHMVGRSIDNVFPRREKIEPGAEVLRLEHWNAY
                     SPSLGRDILKDVNISVRAGEIVGIAGLMGSGRTELAKSIFGNPDHYRVSGTLWVKGDE
                     QKFRSPGDAIHSHLAYVTEDRKGNGLILIDDVRHNITLANLREIATRGVINENEEIVI
                     ASEYKQSLNIKTPSIEQKVRNLSGGNQQKVSLGKWLFVKPGVMILDEPTRGIDVGAKY
                     EIYTIMNSLVDQGMSIIMISSELPEILGMSDRVYVMSAGKIKGELNADEFSQENIMAM
                     AVDY"
BASE COUNT         1407 a         1651 c         1618 g         1188 t
ORIGIN      
        1 taattaggac atttctattt tgctacttta ggacattatc atgttgctct tacactcctg
       61 cctccgcttt tccgcttgac tttaatctgt gttatactat gggacgctcg ggcaatagcc
      121 cgggacacta atcacacacg ccacggcata ggcgctcccg cgtgcccgca agggttcgga
      181 gctgccgtaa agccgggcgg aggcataacg caaaggagta tttatgtcac ccgctatatc
      241 catgaaagcg ctcctcgaga gcggcgtcca tttcggacat cgtaccaaca aatggaaccc
      301 caaaatgcgt ccgtacatct tcaccgaacg caacggtatc catatcatcg acctgcagca
      361 aacggtcaaa ctgctggatg aagccttcaa cgtagtgcgc gacgctgtcg ctgccggcgg
      421 caccatcctc ttcgtcggca cgaaacgcca ggcgcaggaa accatccagg aagaagccga
      481 acgctgcggc atgccctacg tcaaccagcg ctggctgggc ggcaccctga ccaactgggt
      541 caccatccaa cagcgtatcg ttgaacttac ccgcctggag aaactggtgg aaagcggcga
      601 aatcaatcag ctgaccaaaa aagaaggcct gctcatccaa cgcgaaatcg cccgtctgga
      661 aacccgcctg agcggcgtcc gcaacatgaa gcgcctgccc aacctggttt tcatcgtgga
      721 catcgaacgc gaagagaccg ccatgaaaga agcgtatatc aaggaaatcc cgatcattgc
      781 gatggtggac accaactgca atcccagtat gattgattac gtcattcctt ccaatgacga
      841 cgccatccgc gccattaaac ttatcgttgg tcgcatcgcg gacgccgccg aagaaggccg
      901 catgatgcgc aaagaagagg aagtggaaga agtacagccc gaatcccgct ccgtaatttc
      961 ccgcaaaatc gatgaagaca tcgaactggg tgatgacgag ctgctcgggg atgcaacccg
     1021 cgccaagatt cctgccaaag aagagcccga aaccggcgaa acgcccgcag tggaagcctg
     1081 aaccgaacca agaaataaac cagacgcgag actgcttgcg tattgagtaa ggataactat
     1141 gcaaatcacg attgacatga tcaaagaact ccgcgagaaa accggctgcg gaattatgga
     1201 ctgccgctct gccctggaga aatccaacgg caatatggaa caggcaatga tggaactgcg
     1261 cgaaaaaggc ctgaaaacgg ccgaaaaacg cgccgatcgc gtcgcctcgg aaggccgcct
     1321 ggaagtgtac gtccacagcg acggccgcct ggtggtgatg gtcgagctca actgcgaaac
     1381 cgatttcgtc gccaagaccg cttccttcca ggagctggcc cacgaaatcg ccctgcagat
     1441 tgctgccgcc caacccacct acatctccga agcggatatc cctgctgaag tgattgaggc
     1501 cgaaaccaag gccgttgccg agcgcgtgcg cgctgaaggc aagcccgaag ccatcatccc
     1561 caagatcgtg gatggctata tgaaaaagtt caaggacgag aaagtcctcc tgaaccagaa
     1621 atatatccgc gacgaatcca agactgtggc agacctgatt acggacaaag tcagctccct
     1681 gggagaaaac ctgatcgtgc ggcgctttgt gcgctgggcg ctgggagaaa caacccagcc
     1741 gcaggaaagc gctgaatagc agcaaaccag agccaaccaa aagttggctc tatttttagc
     1801 agcaaggagg cagcatgacc gaacaaggca cggcacccaa atacaagcgg atactcctga
     1861 aactctccgg cgagtcgctg gcggggccat ccggttttgg gattgacccc aaggaagcgg
     1921 cacacatcgc caacctggtg aaacgcgtgc acgccctggg ggtagacgtc gcgattgtga
     1981 ttggcgccgg caatctctgg cgcggggcca ccggcgagaa cagcggcatg gaccgcgcga
     2041 ccgcggatta catgggcatg ctcgccaccg tcatcaacgc gctggccttg atggatgcgc
     2101 tggagcgcgt cgaggtcatc acccgggtgc agtccgccat tgaaatgccg actgtagctg
     2161 agccctacat ccgccggcgc gccatccgcc acctggaaaa aggccgcgtg gtcatctttg
     2221 gcgggggaac gggcaacccc ttcttctcca ccgataccgc cgccgccttg cgcgcgactg
     2281 aaattggcgc ggatgtggtc atcaaagcca ccaaagtgga cggcgtatac gacagcgacc
     2341 ccaagacgca ccccgaggcg gtgaagtttg accagcttag ctacatggag acggtcagcc
     2401 gccgcctgag cgtgatggac acgacggctg ttacgctgtg catggagaac gatatcccga
     2461 tcctggtgct gaacctgtgg gagcctgaag cgcttatccg cggcatttta ggcgagagtg
     2521 tcggcacgct ggtcagctgt gattagctga cgaggtttcg ctcccgcgcc tctgcgctca
     2581 gcttaaacag cttggcctct tgagcataat cgtaggtcgg gtggagcggc agcgcaaccc
     2641 gacgggacgt aggcgtagat tgggtggaat ggcaggtcgt aggtcgggtg gagcgtcagc
     2701 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcgggtgga gcgtcagcgc
     2821 aacccgacgg aacgtaggcg taggtcgggt ggagcggcag gtcgtaggtc gggtggagcg
     2881 gcagcgcaac ccgacggaac gtaggcgtag attgggtgga atggcaggtc gtaggtcggg
     2941 tggagcgtca gcgcaacccg acggaacgta ggcgtaggtc gggtggagcg tcagcgcaac
     3001 ccgacggaac gtaggcgtag attgggtggg gcggcagcgc aacccgacaa gcccaggtag
     3061 gttcgtgtat tgccgttggg ttgcgctcct gcgtcgctgc gcccgaccta cacgctgcgc
     3121 tcgaattgcg cgcatttttc aacaaaaaag cagcagcctt tcggctgctg cttttcaata
     3181 caaatccaaa aaattctatt ttcctttgcg ggtagcccga tccacaatca ccgcgccaac
     3241 cagcacagcg ccgcgcacga tatactgaat ggaaatatcc acgcccatca ggttcatgcc
     3301 gctggtcaaa gacatcatta ccagcgcacc gatgatggag ccgggaatgg ttccgacgcc
     3361 gcccgcggcc gagaccccgc cgacgaaggc cgccgcgatg gcatcggtct caaagccgag
     3421 gccggcggtg gtcgtcgcag actgcagacg ggaggcgtac aggatgccgg aaagcgaagc
     3481 cagcataccc atcgcgccaa acacgatgta ggtaatcttg gaaacgctga taccgctcag
     3541 ttcggctgct tcggggttgc cgccaaccgc gtaaatgtgg cgcccgagcg gggtcttggt
     3601 cgtaataaat gtgaacaccg ctacgacgag cagcacaacc accaggctcc acgacagacc
     3661 attgtagccg gagagcaccc aggagacata cccgataatc gctgagatga gcaccagctc
     3721 aacaataaac atcgggacgc tgggcacttt gaagttataa gaaagcttgg tcttgcggcc
     3781 tttaattgcg ctcaggatat agaggatgat tcccagcgcg ccgaccagca aggtcacctt
     3841 gtggttgtta tccaaaaaac caaaatcggg gatatccgga ataaacccgt tgccaatcgc
     3901 gttgaagacc ttgtctttga tgataatcgt gccggttccc atcgtcacca gcagcagcgc
     3961 gccgcggaaa atcagcccgg ctgccatggt agagacgaag gcaggcacac ccagtttggc
     4021 aataggcgtt gaggtcagca gaccggaggc gatgccgagg atgagtacca cggggatgac
     4081 cgcgtacacc ggcagtcccc aggcagtcat cgcgatcgcg gcaaaagcgc cgaggaaacc
     4141 cgccaggtac ccgaccgaaa ggtcgatatg gcggatgacg atgaccaggg tcatgcccac
     4201 agccaacacg gcaatgtacc ccatctgatt aatcaggttg ctgatattac gggaggagat
     4261 aaaaacaccg tcagttgtaa tcgtgaaaaa gatcatgatt acaattagcg cgatgaacat
     4321 gaagtattgg ctgatattgg atttcaacag cccgaccatt tcggcaagga atgaaggtgc
     4381 acgttcttct tgaatgttgg gattttctga catcttgtgt ctcctcaata atcaactgcc
     4441 atagccataa tattttcctg cgagaattca tcggcgttga gttcgccttt aattttcccc
     4501 gcggacatca cataaacccg gtcgctcatc cccagaattt ccgggagctc ggaggagatc
     4561 ataatgatgc tcatcccctg atccacgagc gaattcataa tggtatagat ttcatatttc
     4621 gcgccgacgt cgataccgcg cgtgggttca tccaggatca tcacccccgg cttcacgaac
     4681 agccacttcc ccagcgagac cttttgctgg ttgccgccgc tcaggttgcg aactttctgt
     4741 tcaatggagg gggtcttaat gttgaggctt tgcttgtatt ccgaagcaat cacaatctct
     4801 tcattttcat taatcacgcc ccgggtcgca atttctctga ggtttgccag cgtgatgtta
     4861 tggcgcacgt cgtcaatcag aatcagaccg ttgcccttgc gatcctcggt cacataagcc
     4921 aggtgggagt gaatcgcgtc tcccggactt cggaatttct gctcatcccc ttttacccac
     4981 agcgtacctg agacccggta atggtccgga ttgccaaaaa tgctcttggc aagctcggtg
     5041 cgtccggagc ccatcaaacc ggcgatgccg acaatttcgc cggcgcgcac gctgatgttg
     5101 acatccttga gaatgtcgcg tccgagagat ggcgaatacg cgttccaatg ctccaggcgc
     5161 agcacttccg ccccgggttc gattttctcg cggcggggga agacgttatc aatggagcgc
     5221 ccgaccatat gcttgatcag aacgcggtcg gttacctggt gttccttgtt gtccaaagtg
     5281 cagatcgtct tgccgtcccg caggacggta atggtgtcgg cgatggaaag cacttcgcgc
     5341 agcttatggg aaatgagaat gcaggtgacg ccttgctgct tcagcccctt aatcagctcc
     5401 agcaggttgt cgctgtcggt ttcgttcagg gcagcagtcg gttcatccag aatgaggatt
     5461 ttgacctctt tcttgagcgc cttcgcaatt tcaatcaact gctgcttccc tacgcccagc
     5521 gtcttaacct tgacctccgg agaaacgtcc aaaccaactt tcttgaggat ttcatccgct
     5581 tctttgatgg tctcgttggt gtcaatgatg aagccgttgc ggatttcgtt gcctaaaaag
     5641 atattttcat aaacggataa ttccggaact aacgccagtt cctggtaaat aatcgcaata
     5701 ccagcctttt cactgtcgcg aatgccgtta aatttttgca gctcaccatc gatgaatatt
     5761 tcacctgaat aggagccaaa aggatgtacg ccgctgagga ctttcatcag ggtcgacttt
     5821 ccggctccgt tttcgccaac caggcagtgg atctcgcctc tggccacgct gaaagatact
     5881 ccgtccagcg ctttaacccc aggaaactct ttggtgatgg acttcatttc gaggatcgaa
     5941 tcttccatga gcttctcctg ctta
//
LOCUS       DAOQAD010000111         5951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_16574_length_5951_cov_4.772727, whole genome shotgun sequence.
ACCESSION   DAOQAD010000111 DAOQAD010000000
VERSION     DAOQAD010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16574_length_5951_cov_4.772727"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="PKL60_07695"
     CDS             <1..1575
                     /locus_tag="PKL60_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNW96055.1"
                     /translation="TEIGFSHFASLGNEMDVSEADMLAYFGDDPDVRVIAVYLEGVQD
                     GERFIAEARRVSRIKPIVMLKAGRNNAGARAVSSHTGSLAGSYAAYQAVFKQTGVIEV
                     ETLAELFNVAWALGTQKLPKGKRTAITTNAGGAAALLADNLSANGAELAELSADTQAK
                     LRSKLNPSAQVANPVDMLGGAEPPDYLWSLEQMMRDENVDVLAPVLVPQALVDPLGVA
                     QAWVKAASQTPKTMLTCLMGEHSVREARACLNKAGVPVYQYPDQIGPVLRAMSAYGEY
                     LRKPVFIPVEAKKAHKNTVRAVLEANSGKLSLGEFETRAMLAAYGITNVPGDLAVNGE
                     QAVEIAANIGFPVALKIVAEGVLHKSDVGGILLNLSDGESVRAGFEALLARIRAQFPQ
                     TGIAGAMVEKMAPKGLEVIVGMRRDPTFGPMMMFGMGGTLVEQVKDIAFRVAPLSRGD
                     IAEMIDATIAGKLLGGVRGSARADIEAVEGVITALSQLALDFPEIEEIEINPLVVYPH
                     GQGALALDSRALLRQA"
     gene            complement(1588..1660)
                     /locus_tag="PKL60_07700"
     tRNA            complement(1588..1660)
                     /locus_tag="PKL60_07700"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1625..1627),aa:Thr,seq:cgt)
     gene            complement(1695..2894)
                     /gene="dinB"
                     /locus_tag="PKL60_07705"
     CDS             complement(1695..2894)
                     /gene="dinB"
                     /locus_tag="PKL60_07705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNW96056.1"
                     /translation="MERKILHLDLDAFFCAVEELLDPSLKGKAFAVGGSPKSRGVVSS
                     CSYPARLRGVHSAMPMGRALALCPELIVVRHRFGVYSETSDKVMDILRDYTPLFQQVS
                     VDEAFLDVSDLPQPIAAIARDMQQRIDRELHLPCSIGAATNKLVAKVANDFGKSHVRT
                     GQAPRQITVVPPGTEADFLAPLDVQALWGIGPKTAQRLHSRGIHSIGQLAELPLPELE
                     HFFGKNAAEMQESARGIDRSPVHSGHEVKSVSNELTFARDTSDLDQLLETLRRLSDKV
                     GYRMRQARLMGGVVQIKLRYEDFTTLTRQRALPTPTNLDDEIFGAAEKLLREALAPGR
                     EVRLIGVGVSQLSEPYQQLDLWDSDRSQKEQLALAIDALKDKYGGNIITRASQVKKHP
                     QERQEKD"
     gene            complement(2931..4841)
                     /gene="gyrB"
                     /locus_tag="PKL60_07710"
     CDS             complement(2931..4841)
                     /gene="gyrB"
                     /locus_tag="PKL60_07710"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNW96057.1"
                     /translation="MEEKKHYNAGDIQILEGLEAVRRRPGMYVGGTDIKALHHLIYEV
                     VDNSIDEALAGECDRITVTIHKDESVTVRDNGRGIPVDIHPEKGISALQVVMTVLHAG
                     GKFGGGSYKVSGGLHGVGVSAVNALSEWCEVTVNREGKTYFQRYERGIPQSEVKQIGT
                     YHGSETGTSTTFRFDREIFKGDLSYRFDALISRFREMAFVTRGVTIGLIDERDNREMT
                     FHFEGGIASFVRYLNRTKEVLHEPIYGEKEIDGVGVEFVVQYTDVFAEAVYTFANTIN
                     TIDGGTHLTGLRAAVTRSINDYARKSGLLKDADGNFSGDDTREGMTAIVSIKHPDPQF
                     ESQTKVKLMNPEVQTYVQQVVGDTFTSFLEQNPAAGKAIVQKCLTSARARDAAKKARD
                     LVIRKSALESMTLPGKLADCSERDPSKTELYIVEGDSAGGSAKQGRDRHFQAILPLRG
                     KILNTERARLDKILGNREVKSLISALGTSIGESFDISGLRYNRIVIMTDADVDGAHIR
                     TLLLTFFFRFMLPLIEEGHLYIAQPPLYRISAKNQISYAYSDAEKDSMVARLGGSSKV
                     GISRYKGLGEMNPEQLWETTMNPENRTLLLVTVEDAVEADRTFDMLMGTLVPPRRRFI
                     QTHAKEVRNLDI"
     gene            complement(4860..5846)
                     /locus_tag="PKL60_07715"
     CDS             complement(4860..5846)
                     /locus_tag="PKL60_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96058.1"
                     /translation="MDSRFVLRKQPNVFKAIGAGFKTLGEHLYLLTIPLILDIALLFA
                     PRFTVSDLVSPYLTQLDSLAEASAEIKQLWTELSASLGTFFQSYSLTSALRSYPLGVP
                     SLLSSRVFMDNPFGKLSEIAVSSEGQALLLLLLFGVLGAVLGAVNFYFCSLPTYQEAR
                     KQDAKAPLKSVAGLLLVPLLFWAALLALAIPVSLVVSVLSMLHPFLGLLAYFFVAMTL
                     ISMLLPVLFAPHAVLVLKQNLWQGIRTSIRLLRPYYSATSLFVILAVLASYLTNLLWQ
                     SPGTDSPMVFVGIFGHALVSTALLIGSFHYFIRVLELSQEHELAQQAPESSL"
BASE COUNT         1285 a         1587 c         1821 g         1258 t
ORIGIN      
        1 acggaaatcg ggttttcgca ctttgccagc cttggcaacg aaatggacgt gtctgaagcc
       61 gatatgctgg cctactttgg cgacgatccg gacgtgcgcg tcatagcagt ctatctggaa
      121 ggcgtgcaag acggtgagcg tttcatcgcg gaagcccggc gggtatcacg gattaagccc
      181 atcgttatgc tgaaggcagg caggaacaac gccggcgcgc gggcggtaag ctcacacacc
      241 ggctccctgg cgggttcata cgcggcttac caggctgtat ttaagcaaac cggcgtaatt
      301 gaggttgaaa ccctggcgga attgttcaat gttgcctggg cgctgggcac ccaaaagctg
      361 cccaaaggca agcgcacggc catcacgacc aacgcgggcg gcgccgcggc tctgctggcg
      421 gataatttgt ccgcgaacgg cgcggaactg gctgaactta gcgcggacac ccaggcgaaa
      481 ttgcgcagca aactgaaccc gagcgcgcag gtggcaaacc cggtggacat gcttggaggg
      541 gcggaacccc cggattatct ctggagtctg gaacaaatga tgcgcgatga gaatgtggat
      601 gtacttgcgc ctgtgctggt gccccaggcg ctggtggacc cgctgggcgt ggcgcaggcc
      661 tgggtgaaag cagcctcgca aacaccgaag accatgctta cctgcctgat gggcgagcac
      721 agcgtgcggg aggcccgggc atgcctgaac aaagccggcg tgccggtgta ccagtacccc
      781 gaccagattg gtccggtgct gcgcgcgatg tccgcgtacg gtgaatattt acgtaagccg
      841 gtttttatac ccgtggaagc caaaaaagcg cacaaaaaca cggttcgcgc tgtactcgag
      901 gctaattcag gcaaattgag cctgggtgag tttgaaaccc gcgctatgct cgcggcatac
      961 ggaatcacga atgtgccagg cgatttggcg gtgaatggag agcaagccgt tgaaattgcg
     1021 gcgaacatag gctttcctgt cgctctgaag attgtggcgg agggcgtgct gcacaaatct
     1081 gacgtgggag ggattctgct gaatctaagc gacggggaga gcgtgagagc gggctttgag
     1141 gcgctgctgg ctcgcattcg tgcgcagttc ccgcaaaccg ggatcgcagg cgcgatggtc
     1201 gaaaaaatgg cgccgaaagg cctggaagtt atcgtgggga tgcgccggga ccctacgttc
     1261 ggtccgatga tgatgttcgg catgggcggc acgttggtgg aacaggtcaa ggacattgcc
     1321 ttcagggtgg cgccgctctc ccgcggggat attgctgaaa tgatagacgc gacaatcgcg
     1381 ggaaagctgc tgggaggcgt gcgcggaagc gcccgggcgg atattgaggc ggtggaaggc
     1441 gtgataaccg cgctcagcca gctcgcgctg gacttcccgg agattgagga gattgaaatt
     1501 aatccgctgg tggtgtaccc gcacgggcag ggcgctctcg cgctggacag ccgcgcgctg
     1561 ctgcggcagg cctgattaaa gtaaaaaagc caacgggggg attcgaaccc tcgaccgggc
     1621 gcttacgaag cgtctgctct accgactgag ctacgttggc aatttgccgc attataccaa
     1681 aacctggcaa tatttcaatc tttttcctgc ctttcttggg ggtgtttttt tacctggctg
     1741 gcgcgggtga taatattgcc gccatattta tccttgagcg cgtctatcgc caatgccagc
     1801 tgctcttttt ggctgcggtc gctgtcccac aggtcgagct gctggtaggg ttctgaaagt
     1861 tgactcaccc ccacgccgat caaacgcacc tcacggccgg gggcaagagc ttcccgcagt
     1921 aatttttcag cggcgccaaa gatttcatcg tccaggttgg taggcgtggg gagcgctctt
     1981 tggcgcgtga gggtggtaaa gtcttcgtag cgcagcttaa tctgcaccac accgcccatc
     2041 aggcgagcct ggcgcatgcg gtaaccgacc ttatccgaaa gacgccgcag ggtttccagc
     2101 agctggtcca ggtcgctggt atctctggca aaggtaagct cattgctgac agatttcact
     2161 tcgtgtccgc tgtgtaccgg tgagcggtcg attccccgtg cgctttcctg catttccgcc
     2221 gcgtttttgc caaagaaatg ttccagctct ggcagcggca gttcggcgag ctgcccgatg
     2281 ctgtgaattc cgcgggaatg cagccgttgg gcggttttcg gtccgatgcc ccacaaggct
     2341 tgtacgtcca gcggcgcaag aaaatcggct tctgtacctg gggggacaac tgtaatctga
     2401 cgcggcgcct gaccggtgcg cacgtggctc ttgccgaaat cgttggcgac tttggcgacc
     2461 agcttgtttg tggctgcccc gatggagcag ggcaggtgca gctcgcggtc gatgcgctgc
     2521 tgcatgtccc gcgcgatagc agcgatgggc tgcggcaggt cgctgacgtc caaaaacgct
     2581 tcgtccacgg aaacctgctg aaagagcggc gtatagtccc gcaggatgtc catgaccttg
     2641 tcagaagttt cgctgtacac cccaaagcgg tggcgcacaa caatcagctc agggcaaagc
     2701 gccagcgcgc gccccatggg catggcggaa tgcacgccgc gtagccgggc cgggtaggaa
     2761 caggaggaaa ctacgccgcg ggatttggga ctgccgccta cagcaaaggc tttgcccttc
     2821 agggagggat ccaacagctc ttcaaccgcg cagaaaaacg cgtccaagtc cagatgcagg
     2881 attttgcgtt ccataggctg tcattccaaa gcgcaggccg cgcgcgcaga ttagatatcc
     2941 aggttgcgca cttccttggc gtgggtctgg ataaagcgcc ggcgcggggg tacgagcgta
     3001 cccatcagca tgtcgaaggt gcggtcagct tccaccgcgt cttccacagt aacgagcagc
     3061 agcgtccggt tttcggggtt catggtcgtt tcccacagtt gttccgggtt catttcaccc
     3121 agacctttat accgggaaat accgactttg gaggaaccgc ccaacctggc caccatgctg
     3181 tctttttccg cgtcggaata cgcatagcta atctggtttt ttgcggaaat ccggtagagc
     3241 ggaggctggg caatgtagag gtgcccttct tcaatcaagg gcagcataaa gcggaagaag
     3301 aatgtgagca gcaaggtgcg aatgtgcgcc ccatccacgt cagcatccgt cataataacg
     3361 atgcggttat agcgcagacc ggaaatgtcg aaggactcgc cgatgctggt gcccaaggcg
     3421 gagatgagcg acttgacttc gcggttgccc aggattttgt caaggcgcgc ccgctccgtg
     3481 tttaaaatct tgccgcgcaa aggcaggatg gcctgaaaat gccggtcgcg tccttgtttg
     3541 gcggagccgc ccgcggaatc gccttccacg atgtacagct cggtctttga ggggtcgcgt
     3601 tcggagcaat ccgccagctt gccgggcagg gtcatggatt ccagcgcgga tttgcggata
     3661 accaggtcgc gggctttttt ggcggcgtcg cgtgcgcggg cagaagtaag acatttctgt
     3721 acaattgcct tgccagcggc cgggttttgt tcgaggaagc tggtgaaagt atcgcccacg
     3781 acttgttgga cgtaggtctg aacttccgga ttcatcagtt tgactttggt ttggctctcg
     3841 aactgcgggt cgggatgttt gatgctgacg atggcggtca tgccttcgcg cgtgtcgtcg
     3901 ccgctgaaat tgccgtccgc gtccttcaac aagccgcttt tgcgggcgta atcgttgatg
     3961 ctgcgggtta ccgcggcgcg caggcctgtc aggtgggttc cgccgtcaat ggtattgatg
     4021 gtgttggcaa aggtgtacac tgcttctgca aaaacatccg tatactgaac cacgaattcc
     4081 acgccgacgc cgtcgatttc cttctcgccg tagatgggtt catgcagcac ttccttggtg
     4141 cggttcaggt agcgcacaaa ggaagcaatt ccaccctcaa agtgaaaggt catttcgcgg
     4201 ttgtcgcgct cgtctatcag accaatggtc acgccgcgcg tgacaaaggc catttcccgg
     4261 aagcgtgaaa tcagcgcgtc gaagcggtag gaaaggtcgc ccttgaagat ctcccggtca
     4321 aagcggaaag tggtgctggt gccggtttca ctgccgtgat aggtcccgat ttgcttgacc
     4381 tcggactgcg ggatgccgcg ttcgtagcgt tgaaaataag tttttccttc gcggtttacg
     4441 gtaacttcgc accattcgga aagcgcgttc accgcggaga cgcccacacc gtgcaaaccg
     4501 cccgagactt tgtagctgcc gccgccgaat ttgccgccgg catgcaggac ggtcatgacc
     4561 acctgcaggg ctgagatgcc tttttcaggg tggatatcaa caggaatacc gcgtccatta
     4621 tcccgcacgg tcacgctttc gtctttgtga atggtgacag tgatccgatc gcattccccg
     4681 gccagggctt catcaatcga gttgtcaact acctcgtaga tcaggtgatg cagcgctttg
     4741 atgtccgtac cgccaacgta catacccggg cggcggcgga ctgcttccag cccttccagg
     4801 atttggatat cgcctgcgtt gtagtgtttc ttttcttcca cgtttttctc caaatgtgtt
     4861 cagagagatg attccggcgc ctgctgcgcg agttcgtgct cctggctaag ttccagtacc
     4921 cgaataaagt aatgaaaact gccaatcagg agcgcggtgc tcaccagcgc gtggccgaag
     4981 atcccgacaa acaccatcgg ggaatctgtg ccgggggact gccagagcag attggtcagg
     5041 taagacgcga gcaccgccag gatgacgaac agcgaggtcg cggagtagta aggacgcaac
     5101 aggcggatgc ttgtgcggat gccctgccag aggttttgtt ttagcacaag cacggcgtgc
     5161 ggggcaaaca agactggcag gagcatggat attaaagtca tggctacaaa aaagtacgcc
     5221 agcaatccca ggaacggatg cagcatgctc aacacactca ccaccaggct gactgggatc
     5281 gcgagggcca acagagccgc ccagaacagc agtggcacta ataagaggcc ggcgacggat
     5341 ttgagcggag ctttggcgtc ctgttttcgc gcttcttgat aagtgggcag cgaacaaaaa
     5401 taaaaattga ccgcgcccaa gacagcacca agcacgccaa agagcagcag cagaagaagc
     5461 gcctgccctt cagagcttac agcaatttct gaaagcttgc caaaagggtt gtccatgaag
     5521 acacggctgg aaagcaggct gggaacgccg agcggatagg accgcagggc tgaggtcagg
     5581 ctgtaagact gaaaaaaagt gcccagcgat gcgctcagtt ccgtccaaag ctgcttgatt
     5641 tcagcgctgg cttcagccaa agaatcaagc tgcgtcagat atgggctgac gagatcggaa
     5701 acggtgaagc gcggcgcgaa gagcagggca atatccagga tcagtggaat ggtaagcagg
     5761 tagagatgct caccgagcgt tttaaacccg gctccaatcg ccttgaaaac gttcggctgt
     5821 tttcgtagaa caaatctgct atccataacc ttttaatttt accaccattt ctgttgctgt
     5881 ctttttataa aagcagaggt gtaagagcaa catgataatg tcctaaagta gcaaaataga
     5941 aatgtcctaa t
//
LOCUS       DAOQAD010000112         5852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_16974_length_5852_cov_5.462308, whole genome shotgun sequence.
ACCESSION   DAOQAD010000112 DAOQAD010000000
VERSION     DAOQAD010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16974_length_5852_cov_5.462308"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(517..756)
                     /locus_tag="PKL60_07720"
     CDS             complement(517..756)
                     /locus_tag="PKL60_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96059.1"
                     /translation="MSRLAISLYTLKEVVADLGPIFATKDVSEDPRMKNAHINLVDHP
                     QYHGFVGGALSDHRAQLKIDIVGEDPKRGTRWKKI"
     gene            complement(1059..1832)
                     /gene="surE"
                     /locus_tag="PKL60_07725"
     CDS             complement(1059..1832)
                     /gene="surE"
                     /locus_tag="PKL60_07725"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNW96060.1"
                     /translation="MKILVTNDDGVTAPGLLALAQALRGLDAEITVLAPDHNWSASGH
                     VKTMHRPLRLSATILADGSQAWKSDGAPSDCVALAMMGALESDFDLVVSGINPNANIG
                     LDVTYSGTVTAAMEASIWGKPGFAISLDAPEYHEGPLDFRSAAETAARLVWQLLPEAN
                     GKQLAPIFNINIPYRPDGDYAGVMLTRQGVRIYRDVLEKRLDPRGRPYYWIAGDPPSG
                     VKEDGTDYGALQEGYVSITPLKMDMTDFAALEKLRSVRV"
     gene            complement(1849..>5852)
                     /locus_tag="PKL60_07730"
     CDS             complement(1849..>5852)
                     /locus_tag="PKL60_07730"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96061.1"
                     /translation="NLLAGDQHKRTISKMVSVVDEINALEPSFEALSAAALRAKTDEF
                     RARLSDGESLDALLPEAFAAVREASKRTLGMRHYDVQLICGINLHQGSISELRTGEGK
                     TLAATLPLYLNALEGKGAHLITVNDYLARRDGRWMGAIYHLLGLQVGILQMSNSADGS
                     QLAYLYDPDEHSLREESNLLRPVRRSQAYLADITYGTNSEFGFDYLRDNITMSWEGRV
                     QRGHHYAIVDEVDNILIDEARTPLIISGPSHEDAEYYIRMAQVARALNPEDYEVDEKD
                     RTVTLTESGVAHVEELLGTPLSDPDRPEDISPEQARLMGFLEQSLRAQYLFHRNKEYI
                     VQNGEVIIVDEFTGRMMPGRRWSDGLHQAVEAKEGVKVQAENVTHATITIQNYFRMYS
                     KLGGMTGTALTEKEEFYRIYGLDVLAIPTNLEYRASRPETGLSELEARDEEGYRYSYY
                     ANSADPQKKPLFFKRKDYPDVIYRTAEAKLRAIVLEIIRFHVLGRPQLVGTTSVESSE
                     RLSDRLAAEPVRRLLQTVLIREAWRQKVNPKGDDFSAPKELAVLNEPLTKLRMPELRK
                     LSAQYGIETLDLADNSNRALLLDALHLADADWERLQPLFDAGIPHQVLNARKHTEESI
                     IIAGAGAFGAVTIATNMAGRGVDIKLGGEIPESLLVQVNLVLAAIAKIDPYSLTMEQR
                     LDAVQTLNGDLSEEQREAVERFKVYMADMERVRALGGLHVIGSERHEARRIDNQLRGR
                     AARQGDPGSSRFFLSLEDELMRLFGGNQVDTLLTRFKFDENMPIEANLIGRLVEQAQT
                     RVEGANFDVRKHLLEYDDVLNAQRTRIYAQRDLIFNKADLREDVTEMLRTELGPRVKA
                     GVEDPEGPWKLLAYLEDVQPNLNTPWVSFPSFPYKLALQQLGKPTDEADLTRRILALA
                     EEAVTAEHTHLLGNINALLDKSEQTLKNQIAERSEALDAYLQTFDPDEPHDLQSEIST
                     LLQVPVRLPQAQQKALLEDSESMKAPLLNALKTGLMLTLTRRLLLTLERRFGETWALK
                     ATELATQPWPDVRKQILDQVNLMTERRAKRLLGPDGEIARDLAANAEHLTLALDDEIE
                     RMRLLQLTTQGTRVTFDAKSHRRQLTGTLRMTYIFSLANQLETIPLKDLSEQVLAHLE
                     EAETVFVTVFGNAEWEHLRANNFTLESLPQNTRDSLSAQLGAEKLAGLQTLPLEEIPE
                     EEKPIVVQELGRAAQNRIYRQLLLGTITETWVEYLTKMEALRVSITMESYAQRDPLVQ
                     YKSQASTMYTELFSEVRQTVISRMFRYRPNLPADFSGTLTGAGVPAPVPGGGGQTAPQ
                     ANAAQKQKRRKRH"
BASE COUNT          998 a         1667 c         1882 g         1305 t
ORIGIN      
        1 tttcttcaaa acgcagagaa tgagcgctag tctgtcatcc tgaatggagt gaaggatctg
       61 tgctttgttg cctttgagat tcttcgctgc tccaagaatg ataaggattt ttgtcatcct
      121 gaacggagtg aagaatctgt gctttgttgc ctttgagatt ctcagcttcg ctcagaatga
      181 ccgcttcccc gttatcgcgt gcgcacgcgc gcggtctgga taccggcgcg agcagggttg
      241 gaaggataaa aattcgtcat cgcgagcgca gcgcggcgat ctccctgttt gctccctgta
      301 agcctgtagg cagattgcca caccgttttg ccttagatcc cggcaggtta ggcttcacga
      361 tagggggcaa aacggttcgc aacgacgagt tatttgttat cgtgagcgca cgcgcgcggt
      421 ctggagaccg gcgcgagcgg ggggcttcgc tcagaatgac cgcttccccg ttatcgcgtg
      481 cacacgcgcg cggtcaggag accggcgcga gcagggtcag attttcttcc aacgagtccc
      541 acgtttgggg tcttcgccaa cgatatcaat ctttaattgt gcgcggtggt cgctcaaagc
      601 tccacccaca aatccatgat attggggatg atctacaaga tttatgtgag catttttcat
      661 tcgtggatct tctgaaacgt ctttggtggc gaaaattgga ccaagatctg caacaacttc
      721 tttcaacgtg tacaatgaaa tcgctaatct actcatcctg ccctctttca tttgtctgaa
      781 taacttaaca ggcttcctta gtggtctatg aacaaaaata tgtttattac aatcctctgt
      841 tctagcatgg tctcctgact atgcaaggaa agtgaccttt agttatcgaa aaagctattg
      901 gctatcttat taatgttcag tgcaattctg atgtaatacg acctttggcc acacctctcg
      961 cgcggtctgg agaccggcgc gagcagggga aaatgaatag attcttcgct tcgctcagaa
     1021 tgactgatca gcttaccaac caggtcagaa cgcgccccct acacccgcac gctccgcagc
     1081 ttctccagcg cggcaaagtc ggtcatgtcc atcttcagcg gggtgatgga cacatagcct
     1141 tcctgcagcg cgccgtagtc cgtgccgtct tccttcacac ccgaaggcgg gtcgccggca
     1201 atccaatagt aaggtctgcc gcgcgggtcc aggcgtttct cgagcacgtc ccggtaaatg
     1261 cgtacgccct ggcgggtgag catcacgcca gcgtagtccc catccggccg gtaagggatg
     1321 ttgatgttga aaatcggcgc aagctgtttc ccgtttgcct cgggcagcag ctgccagacc
     1381 aggcgcgcgg cggtctccgc cgcggaacga aagtccagcg gcccctcatg gtattcgggc
     1441 gcgtccagtg agatggcaaa acccggtttg ccccaaatcg aggcttccat cgcggcggta
     1501 acggtgccgg agtaggttac gtccagcccg atgttggcgt tcgggttaat cccggagacg
     1561 accaggtcaa aatcgctttc cagcgcgccc atcatcgcca gcgccacgca gtcggatggc
     1621 gcgccgtcgg atttccaggc ctgggagcca tccgccagga tggtcgcgga gagcctgagg
     1681 gggcggtgca tggttttgac gtgcccggaa gccgaccagt tgtggtccgg ggcgagcacg
     1741 gtaatttccg cgtccagccc gcgcagggcc tgggctaagg ccagcaagcc gggggcggtc
     1801 acgccgtcat cattggtaac cagaattttc atgccttcct ccgtacggtt aatgacgctt
     1861 gcgccgtttt tgtttttggg cagcgttggc ctgaggcgcc gtttggccgc cgcccccagg
     1921 cacaggcgca ggaacccccg cgccagtcaa cgtgccgctg aagtctgccg gcaggttcgg
     1981 gcggtagcgg aacatgcggc tgatgacggt ctgacgaacc tctgagaaga gctccgtgta
     2041 catcgtgctg gcttgtgact tgtattgcac cagcgggtcg cgctgggcgt aggattccat
     2101 ggtaatggac acgcgcaggg cttccatctt cgtcaggtat tccacccagg tctcggtgat
     2161 ggtgccgagc agcaattggc ggtagatgcg gttctgggca gcgcgcccca gctcctgcac
     2221 cactatcggt ttttcctctt cggggatttc ctccagcggg agggtttgca gcccggctaa
     2281 tttttccgcg ccgagctgag cgctgagcga gtcgcgcgtg ttttgcggca gggattcaag
     2341 cgtgaagttg ttcgcgcgca ggtgttccca ttcggcattc ccaaacacgg tcacgaagac
     2401 ggtttcagct tcttccaggt gcgccaaaac ctgttcgctc aggtctttga gcggaattgt
     2461 ttccagctgg ttggcgagtg agaagatgta ggtcatgcgc agggtgcctg tcagctgccg
     2521 gcggtgggat ttggcgtcaa aggtgacgcg cgtcccctgg gtggtcagct gcagcaggcg
     2581 catgcgctca atttcatcat ccagcgccag cgtcaggtgt tcggcatttg ctgccaggtc
     2641 ccgcgcgatt tcgccatccg gtccgagcag gcgtttggcg cggcgctcgg tcatcaggtt
     2701 cacctggtcc aggatttgct tgcgcacatc cggccaaggc tgagtggcca gctcggtggc
     2761 tttcagcgcc caggtctcgc caaagcggcg ttccagggtg agcagcaggc ggcgcgtgag
     2821 cgtgagcatc aggccggtct tcagcgcgtt caagagcggc gctttcatgc tctcggagtc
     2881 ttccagcaga gccttctgtt gggcctgggg caggcgcacg ggcacctgca gcagagtgga
     2941 aatctcggac tgcaagtcgt gcggttcatc cgggtcgaag gtttgcaggt aagcgtccag
     3001 cgcctcgctg cgctcagcga tttgattctt gagggtctgt tcggatttgt ccagcagggc
     3061 gttaatgttg ccgagcaggt gggtgtgctc cgctgttaca gcctcttccg ccagcgcgag
     3121 gatgcggcgg gtcaggtcgg cttcgtcggt gggcttgccc agctgctgca gcgccagctt
     3181 gtaggggaag gacgggaaac tgacccaggg cgtgttcagg ttgggctgga cgtcctcgag
     3241 atatgccagc agcttccagg ggccttcggg gtcttccacg ccggctttca cgcgcggacc
     3301 gagctcggtg cgcagcattt cggtaacgtc ctcgcgcagg tcggctttgt tgaatatcag
     3361 gtctcgctgg gcataaatcc gggtgcgttg ggcgttcagg acgtcgtcat attccagaag
     3421 atgtttgcgg acgtcaaagt tggcaccttc cacgcgggtt tgggcttgct ccaccaggcg
     3481 gccaatcagg ttggcttcga tgggcatgtt ctcgtcaaac ttgaagcgtg tcagcagcgt
     3541 gtccacctgg ttgccgccaa acaggcgcat cagctcgtct tccagcgaga ggaagaaacg
     3601 cgaggagccg gggtcgccct ggcgggcggc gcggccgcgc agctggttgt cgatgcggcg
     3661 ggcttcgtgg cgttcggagc cgatgacgtg caaaccgccc agggcgcgca cgcgctccat
     3721 atcggccatg tagactttga aacgctcgac cgcctcgcgc tgttcctccg acaggtcgcc
     3781 gttcagggtc tggaccgcat ccaggcgctg ttccatcgtc aggctgtagg ggtcaatctt
     3841 cgcgatggct gccaggacca ggttcacctg caccagcagg ctctccggga tttccccgcc
     3901 gagtttgatg tccacgccgc gcccggccat attggtcgca atcgtcaccg cgccaaaggc
     3961 gccggcgccg gcgatgatga tcgattcttc ggtgtgtttg cgcgcattca agacctggtg
     4021 cgggatgccc gcgtcgaaca agggctgcag gcgctcccaa tccgcgtcag caagatgcag
     4081 cgcatccagc agcagcgccc ggttagagtt gtctgccagg tccagcgttt cgatgccgta
     4141 ctgcgcggaa agtttgcgca gttcgggcat gcgcagtttg gtcaggggtt cgttcaggac
     4201 ggccagctcc ttgggcgcgc tgaagtcatc acccttgggg ttgaccttct gccgccaggc
     4261 ctcgcgaatt aaaacggtct gcagcaggcg ccgcaccggt tccgccgcca ggcggtccga
     4321 gagccgttcg gagctctcca cggaggtggt gccaaccagc tgcgggcgcc cgaggacgtg
     4381 aaagcggatg atttccagca cgatggcgcg cagtttggct tccgcggtgc ggtaaatcac
     4441 atccgggtag tccttgcgct tgaagaagag cggctttttc tgcgggtccg cgctgttggc
     4501 gtagtaggaa tagcggtagc cttcttcgtc gcgggcttcc aactcactca ggccggtctc
     4561 ggggcggctg gcgcggtatt ccaggttggt agggatggca agtacatcca ggccgtagat
     4621 gcggtagaac tcttccttct cggtcagggc tgtaccggtc atgccgccca gcttgctgta
     4681 catgcggaag tagttttgga tggtgatggt ggcgtgggtc acgttttctg cctggacctt
     4741 cacgccttcc ttggcttcca ccgcctggtg cagcccgtcc gaccagcgtc ggccgggcat
     4801 catgcggccg gtaaattcgt ccacgatgat gacttcgccg ttctggacga tgtattcttt
     4861 attgcggtgg aacaggtact gcgcgcgcag gctctgctcc aaaaagccca tcaggcgggc
     4921 ctgttccggg ctgatatcct ccgggcggtc gggatcgctg agcggcgtac cgaggagctc
     4981 ctcgacgtgc gccacgccgc tttccgtcag cgtgaccgtg cggtcctttt cgtccacctc
     5041 gtagtcctcg gggttgagcg cgcgcgccac ctgcgccatg cggatgtaat actctgcgtc
     5101 ctcgtgcgag gggccggaga tgatgagcgg ggtgcgggct tcgtcgatga ggatgttatc
     5161 cacttcatcg acgatggcat agtggtgccc gcgctgcacg cggccctccc agctcatggt
     5221 gatgttatcg cgcaggtagt cgaagccgaa ctcgctgttc gtgccgtagg tgatatccgc
     5281 gaggtaagct tgcgagcggc gcaccgggcg cagcaggttg ctttcctcgc gcagggaatg
     5341 ttcgtccggg tcgtacaggt atgccagctg gctgccatcg gcagagttgg acatttgcag
     5401 gatgccgacc tgcaggccca gcagatggta aatcgcgccc atccagcgcc cgtcgcggcg
     5461 ggccaggtag tcgttgacgg tgatgaggtg cgcgcccttg ccttcgaggg cgttcagata
     5521 gagcgggagc gtggcggcca gggtcttgcc ctcgcccgtg cgcagctcgg agatgctgcc
     5581 ctggtgcagg ttgatgccgc agatgagctg cacgtcgtaa tggcgcatgc cgagcgtgcg
     5641 cttgctggct tcgcgcacgg cggcaaaggc ttccggtagg agcgcgtcca ggctttcgcc
     5701 gtcggagagg cgcgcgcgga attcgtctgt cttggcgcgc agggctgcgg cgctgagggc
     5761 ttcgaaggaa ggttcgaggg cgttgatctc gtcgactacg gagaccattt tggagatggt
     5821 gcgtttgtgt tggtcgccgg cgaggaggtt gg
//
LOCUS       DAOQAD010000113         5839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_17026_length_5839_cov_5.901798, whole genome shotgun sequence.
ACCESSION   DAOQAD010000113 DAOQAD010000000
VERSION     DAOQAD010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17026_length_5839_cov_5.901798"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(365..1693)
                     /locus_tag="PKL60_07735"
     CDS             complement(365..1693)
                     /locus_tag="PKL60_07735"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HNW96062.1"
                     /translation="MQPLNLPGETARAYIERDSQVISPSYPRGYPFVMDHGRGTEVWD
                     VDGNRFLDFEAGIAVASTGHSNPRVVKAIQEQAEKFIHISSDFYHDKWITLAEKLDSL
                     APWKGPSCCFMTNSGTESVEAAIKLARYHTGSTQFIGFLGAFHGRTLGAVTFTASKAN
                     YKGGFFPLMNGVVHVPFPNPYRPLLQRRPGMGDGETVVHYIEEEILGHLVPGDDVAGI
                     LIEPVQGEGGYIFPPDDFFPALRDLCDRHDILLIVDEVQSGMGRTGKWWAIEHFGVEP
                     DILLTAKGIASGMPLGAMVTKKHVMDWPKGSHGNTYGGNPISCAAALATIQLIEEEYL
                     ENTRVVGAYCKDVLEELQARHPSIGDVRGKGFMLGVEFVLDRETKQPAEKLRDDIVNK
                     AFERGLMTLGCGKSVIRITPPLSTSKSEVDEAMLILDEAIGLAEKAHQLI"
     gene            1892..3262
                     /gene="yvcK"
                     /locus_tag="PKL60_07740"
     CDS             1892..3262
                     /gene="yvcK"
                     /locus_tag="PKL60_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HNW96063.1"
                     /translation="MTPPKNPAIIQTMNDSGSKDRQKHQLWKKLKEEARWLLPGTGFK
                     RWILLTILGSMLLGLGLAVLILGYYRSAANPTMLPILAFLSLRWLDRPIRFVLFGGLG
                     LAMIVVGIWGANRSLLKPFQRSGRPLIDTIQSYRRREKGMRVVVIGGGHGLATLLRGL
                     KEYTHNITAIVTVADNGGSSGELKRDMGILPPGDIRNCLSALSSDEALLSQVFQYRFQ
                     TGTGLTGHSFGNLFISALSEITGSFEEAVAESGRVLAIYGQVLPATISNVELIGEIQD
                     PQTQQLIRVSGESELTNTPGSIKRVWLDPANTPAYPPAVQAILSADLIILGPGSLYTS
                     LLPNLLVTGLADALEASRAQKYFVCNVATQRGETDGFNASDHLHVIEQHIGRDLFDLV
                     VCNRSFRGKLEQDIEWVIADVPLHENYHVYEADLVDDLYPWRHDSKKLARAVVNLFEE
                     RTGPLS"
     gene            3363..4409
                     /gene="gap"
                     /locus_tag="PKL60_07745"
     CDS             3363..4409
                     /gene="gap"
                     /locus_tag="PKL60_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNW96064.1"
                     /translation="MKTKVGINGFGRIGRQVLKAIMERAGDELEVVAINDLFDAQTNA
                     HLFKYDSNYGAFPGTVEVVDGNLVIDGKTIKVFAEKDPGNLPWKDLGVDIVVESTGIF
                     TNARSENGKVGADAHILRGGAKKVIISAPAKGEDLTIVLGVNEEKYDPAVHHILSNAS
                     CTTNCLAPAAKIVHDKFKILNAVMTTIHSYTNDQVILDQGHKKELRRSRAAGLNIIPT
                     TTGAAKAVALVIPELKGKFDGYSLRVPTPTVSIVDFVATIEKPTTKEELNAAFVAAAN
                     GPLKGILGVTTGEYNQPLVSSDFKGDPRSSIVDLPNNMVMGGNLIKVVTWYDNEWGYS
                     CRTADLAKLVAKSL"
     gene            4496..5671
                     /locus_tag="PKL60_07750"
     CDS             4496..5671
                     /locus_tag="PKL60_07750"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNW96065.1"
                     /translation="MFNKKLVTDLDVKGKKVLVRVDFNVPIKEGKVKDATRITAALPT
                     IQYLLDHGAAVILCSHLGRPKDAPDPAYTLKPVADYLATLISAPVKFAQDCIGPVAEA
                     ASAALKPGEVLVLENTRFHAEETKNDPAMSKALASLADLYVNDAFGSAHRAHASTAGV
                     AEYLPSAAGFLMEKEIRYLGNAIANPTRPFVAILGGAKISDKIGVIENLLKKADKILI
                     GGGMANTFLAAQGYQLADSLVEAEVLDQARALLAKSEGKLLLPRDLVVADAFDADANK
                     QTLPLGDVPAGWRVLDIGPETVKAFAAEIAKAGTVVWNGPMGVFEFPRFAEGTFAVAK
                     AVAESSAVSIIGGGDSVAAINQSGLSDKITHISTGGGASLEMLEGLELPGVALISDK"
     gene            complement(5660..>5839)
                     /locus_tag="PKL60_07755"
     CDS             complement(5660..>5839)
                     /locus_tag="PKL60_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96066.1"
                     /translation="GRLPHPLLRISTLVEFQQRIALPAGIPSGRLINPAHIEINKAAP
                     ETEAASFKNKRALLV"
BASE COUNT         1253 a         1765 c         1602 g         1219 t
ORIGIN      
        1 gttgggtttc gctcccgcgc ttctgcaccc ggcttatgcg gcttgtcatc ctgagcgttt
       61 cgttctgtca ttctgagcgc agcgaagaat ctctatgcca aaacgaccag ttccttcgtg
      121 ggctcaggcg cacaaacaaa ggcgcgcagg gcagcgaacg attcctcgtt tcaagccccc
      181 aattccgcat taagcctgaa aaagttcgtc gcgctgagcc cccgcaggaa tcatttcctg
      241 agggtggaaa ccacgaggag ggctctcgcg gtatcctcca aaccggattt ctttgcctga
      301 gggttgcaat cgaaaaaaag cgcggccgtc tgcagccgcg cctgtgtttt cgtctgattt
      361 tcccttagat aagctgatgc gctttttccg ccaaaccaat cgcttcgtcc aaaatgagca
      421 tcgcttcatc cacctcagac ttgctggtgg aaagcggtgg ggtgatgcga atcacgctct
      481 tcccgcagcc cagggtcatc agcccgcgct cgaaggcctt attgacaata tcgtcacgta
      541 atttttccgc cggctgcttt gtttcccggt ccagcacaaa ttccaccccc agcataaaac
      601 ccttgccgcg tacgtcccct atgctgggat gcctggcctg cagttcctcc agcacgtctt
      661 tacaatacgc gcctaccacg cgggtgtttt ccaggtattc ttcttcaata agctggatgg
      721 tggccagggc ggccgcgcag gaaatcgggt tgccgccgta ggtattcccg tgcgagccct
      781 ttggccagtc catcacgtgt ttttttgtaa ccatcgcgcc caggggcatg ccggaagcga
      841 tgcccttggc ggtcagcaga atgtccggtt ccacgccaaa atgctcaatc gcccaccatt
      901 tgccggttct gcccatcccg gattgcactt catccacaat caggaggatg tcatgccggt
      961 cgcacaggtc gcgcagcgcc gggaaaaaat catcaggcgg gaagatgtaa ccgccctccc
     1021 cctgcaccgg ttcaatcaag atgccggcca cgtcgtcacc gggcaccagg tgccccaaaa
     1081 tttcctcttc aatatagtgc accacggtct cgccgtcccc catgcccggg cggcgctgca
     1141 gcagcgggcg gtatgggttg gggaagggca cgtgcaccac gccgttcatc agcgggaaaa
     1201 agccgccttt gtaattggct ttgctggcgg taaaagtaac cgccccaagt gtgcgtccgt
     1261 gaaaagcgcc caggaagccg atgaactggg tgctgccggt gtggtaacgc gccagtttaa
     1321 tcgccgcttc cacactttcg gtgccggaat tggtcataaa gcagcaggag ggacccttcc
     1381 agggcgcgag ggaatccagt ttctccgcca gcgtaatcca cttgtcatgg taaaaatcgg
     1441 atgagatatg aatgaatttt tcagcttgtt cctggatggc tttcaccacg cgcggattgc
     1501 tgtgaccagt ggacgcaacc gcgatgccgg cttcaaaatc gaggaagcgg ttgccgtcca
     1561 catcccagac ctcggttcct cgcccgtggt ccatcacaaa ggggtacccg cgcgggtaag
     1621 agggggaaat aacctggctg tcccgttcaa tgtaggctcg cgccgtttct cctggtaagt
     1681 tcaaaggctg catagctggc tctcctttca gcagaaaagt aaacaaaaag aaccaaagct
     1741 ggtgagaaag gcttggttct tcttcaggaa atcatgcaaa tagtatacca taggccgccg
     1801 gcaaactgcc gcatccgttc gcgcgcgggc gtccacgcat ccgtccgcgc atccgtccgc
     1861 gcgcgggcgt cctatcaggg ctgcgcggta gttgacaccc cccaaaaatc ctgctatcat
     1921 ccaaacaatg aacgattccg gcagcaaaga caggcaaaaa catcagctat ggaagaagct
     1981 taaggaagaa gcccgttggc tccttcccgg cacaggtttc aaacgctgga ttctactcac
     2041 catcctgggc tcgatgctgc ttgggctggg gttggcggtg cttatcctgg gttactaccg
     2101 ctccgctgcc aacccgacca tgctacccat cctggcattc ctctccctgc gctggttgga
     2161 ccgtcccatc cgcttcgtcc tgtttggcgg gctgggattg gcgatgattg tagtcggcat
     2221 ctggggcgcc aaccgctcct tgctgaagcc tttccagaga tccggccgcc cgctgataga
     2281 taccatccag tcttaccgcc gacgcgagaa aggcatgcgc gtggtggtca ttggcggagg
     2341 tcacggcctg gcgacgctgc tgcgcgggct gaaggaatac acgcacaaca tcaccgccat
     2401 cgtcaccgta gcggataacg gcggctcttc cggtgaattg aaacgcgata tgggcattct
     2461 ccctcccggc gacatccgca actgcctgtc ggcgctcagc agcgatgaag ccctgctctc
     2521 ccaggtgttc caataccgct tccagaccgg caccggtctg accgggcatt cgttcggcaa
     2581 cctcttcatc tccgcgctga gcgagattac cggaagcttt gaggaagccg tagccgaatc
     2641 cgggcgggtc ctggcgattt acggccaggt cctgcctgcc accatttcga atgtggagtt
     2701 aattggcgaa attcaggacc cacagactca gcagcttatc cgcgtaagcg gcgaatccga
     2761 gttgaccaac acgcccggca gcatcaagcg cgtctggctg gacccggcca acacccccgc
     2821 ttatccgccg gctgtgcaag ccatcctcag cgccgacctg attatcctcg ggccgggcag
     2881 cctgtatacc agtctgctgc cgaacctgct ggtcaccggc ctcgcagacg ccctggaagc
     2941 cagccgcgcg cagaaatact tcgtgtgcaa cgtcgccacg cagcgcggcg aaacagacgg
     3001 tttcaacgcc tctgaccatc tccatgtcat tgaacaacac attggccggg acttgtttga
     3061 cctggtggtt tgcaaccgca gtttccgcgg caagctcgag caagacatcg aatgggtgat
     3121 tgccgacgtg cccctgcacg aaaattacca cgtctacgaa gccgatttgg tcgacgattt
     3181 gtatccctgg cggcatgatt ccaagaaatt ggcgcgcgcg gtggtaaact tattcgaaga
     3241 gcgcaccgga ccgctgtcct gattgtttca accttagact ctgctaagaa tttcttttct
     3301 aatcgaccgc gcaggtggta aaataaattc gttaacactc aatattccat ggaggatttt
     3361 ctatgaaaac aaaagtaggt attaacggct ttggtcgtat cgggcgccag gtcttaaagg
     3421 cgatcatgga acgtgctggt gacgaactgg aagtggtcgc catcaacgat ttgttcgatg
     3481 cccagacgaa cgcgcatctc ttcaaatacg actccaacta cggcgctttc cccggcaccg
     3541 tggaagttgt ggacggcaat ctcgtcattg atggcaagac catcaaagtc ttcgcggaaa
     3601 aagacccggg caacctgccc tggaaagacc tcggcgtgga tattgtggtt gaatctaccg
     3661 gtatcttcac caatgcccgc tctgaaaacg gtaaggtcgg cgcggatgcg catatcctgc
     3721 gcggcggcgc caagaaagtc atcatctccg cgcccgccaa aggcgaggac ctcacaatcg
     3781 tcctgggcgt caatgaggaa aagtacgacc cggctgtgca tcacatcctc agcaacgcct
     3841 cctgcaccac caactgtctg gctcccgccg ccaagattgt gcatgataaa ttcaaaatcc
     3901 tgaacgcggt catgaccacg atccactctt acaccaacga ccaggtcatt ctggatcagg
     3961 gccacaagaa ggaattgcgc cgctcccgcg ctgccggtct gaatattatc ccgaccacta
     4021 ccggcgcagc caaagctgtc gcgctggtta tccccgagct gaagggcaaa tttgacggct
     4081 attccctgcg cgtcccaacc cccacggtct ccatcgtgga ttttgtcgcg accatcgaga
     4141 aacccaccac caaagaagaa ctgaacgctg ccttcgtggc ggctgccaac ggcccgctga
     4201 agggcatcct gggcgtcact accggcgaat acaatcagcc gctggtctcc tccgacttca
     4261 agggcgaccc ccgctcctcg attgtggacc tgcccaacaa catggtgatg ggcggcaacc
     4321 tcatcaaagt cgttacctgg tacgataatg aatggggtta ttcctgccgt actgccgatt
     4381 tggccaaact cgttgccaaa tccttgtagt ccgctgcccg ggcactgaag atgagagtgc
     4441 gcgaagctgt tctgaaaaga cagtggagcg cactttttta accaaggagg ctttgatgtt
     4501 taacaagaaa ctggtcaccg acctggacgt gaaaggcaaa aaagtgcttg tacgcgtgga
     4561 cttcaacgtc ccgattaagg aaggcaaagt gaaggatgcc acccgcatca ccgccgcgct
     4621 gcccaccatt caatacctgc tcgatcacgg cgctgccgtc atattatgtt cccatctcgg
     4681 ccgcccgaag gacgcgccag accccgccta cacgctgaaa cctgtggcgg attacctggc
     4741 cactctcatt tccgcgccgg tcaaattcgc gcaagattgc atcggcccgg tggccgaagc
     4801 cgcctctgcc gcgctgaaac ccggcgaagt gctggtgctc gagaataccc gcttccatgc
     4861 cgaagaaaca aaaaacgacc ccgccatgtc taaggcgctg gcttcgctgg cagacctgta
     4921 cgtcaacgac gctttcggct ccgcgcaccg cgcgcatgcc tccaccgccg gcgtggcgga
     4981 atacttgccc tcggctgccg gctttttgat ggaaaaggaa atcaggtatc tcggcaacgc
     5041 tatcgccaac ccgacccggc cttttgtggc catcctgggc ggcgccaaaa tcagcgataa
     5101 gatcggcgtg attgagaacc tgctcaagaa agccgacaaa atcctgattg gcggaggtat
     5161 ggcaaacacc ttcctggctg cccagggcta ccaactggcg gactccttgg tggaagcgga
     5221 agttttggac caggcccgcg cgctgctggc aaaatctgaa ggcaagctgc tgctgccccg
     5281 cgatttggtc gtcgcagatg cctttgacgc ggacgccaac aaacagaccc tgccgctggg
     5341 ggatgtgcca gccggctggc gtgtgttgga tatcggcccg gaaaccgtga aagcctttgc
     5401 agccgaaatc gccaaagccg gtacggtagt ctggaacggc cctatgggcg tgtttgagtt
     5461 cccgcgcttc gcggagggca cttttgccgt ggcgaaagcg gtggcggaaa gcagcgccgt
     5521 cagcatcatc ggcggcgggg attccgtggc cgccatcaac caatccggcc tgagcgataa
     5581 aatcacgcac atctccactg gcggcggcgc ctcgttggaa atgctggagg ggcttgagct
     5641 ccccggcgtg gcgttgatat cagacaagta aagcgcgttt gtttttgaat gaagccgcct
     5701 ccgtttcggg ggcggcttta ttgatttcga tgtgggcagg attaatgagc cgtcccgagg
     5761 gaatgccagc cggaagcgcg attcgctgct ggaattccac gagtgtggaa atcctaagca
     5821 gggggtgcgg caatctgcc
//
LOCUS       DAOQAD010000114         5832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_17062_length_5832_cov_6.728752, whole genome shotgun sequence.
ACCESSION   DAOQAD010000114 DAOQAD010000000
VERSION     DAOQAD010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17062_length_5832_cov_6.728752"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..1042)
                     /locus_tag="PKL60_07760"
     CDS             complement(227..1042)
                     /locus_tag="PKL60_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNW96067.1"
                     /translation="MNSLKKILLPFVIIALMLVSSVLLWSAVSSRPASAQAVYQTPTP
                     DAEGRIYYTVEEGDTCTRVFLLTNVSIDDIIRLNGLTEDCNLITGTKILLGTVAAPEL
                     TQTAMPSPTIAPELITPTPTPEPGFAKVCIVLFNDLDGTGMRTEGETYLYGGVVSIND
                     RLGKVSVTGTTVAGNPDEVDPLCFENIPEGSYNITVAIPDGFNATTITNYPLEVKAGE
                     QATIDFGAQVATAAPGEDAAGASVKSPILGIVGIVVLAAGIGLGFVMWRQRKV"
     gene            1110..1925
                     /locus_tag="PKL60_07765"
     CDS             1110..1925
                     /locus_tag="PKL60_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96068.1"
                     /translation="MSAPRGGRIGYNKAMKSLRACFLCLLAAALLLGACLPQQAAPTP
                     GPTLPPTRTAQPSPTIDWFPDTPTPTQRVYEVTPNPLEGLTPPQYAQVLYEEDFSGGE
                     QWKESSGADGNIRYGSGALALALTGKKAEISSLSKIMLPDSFYLELTLESALCGANDS
                     FGILFWRFSELGTYRMLYRCDGQARLERAIYDGVSVLQNWTALRRFQPNAPALNRLGI
                     WADQGTLYFFVNDTFQFSAEGRKGLSGALGVFASASGEQAATFNVTSLRITQP"
     gene            2091..3104
                     /locus_tag="PKL60_07770"
     CDS             2091..3104
                     /locus_tag="PKL60_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNW96069.1"
                     /translation="MPETLKIIIPMAGLGSRLRPQTWSRPKPLISLAGSTVLDHMLSM
                     FKTVPDSEHVEYVFIMSPGQRELIHAHMQKFHPRKQVHYVEQPEKKGQSDALWHAREY
                     LKGRLLVVFSDTLIENSFAFLNNEKLDSVAWVKAVPDPRRFGVAETGALGLVRRLIEK
                     PNDMNNNLAVVGCYYFKEGETLLAAVEEQFRRNISLKGEYYLADALNIYLEQGAVMRT
                     EQVSVWLDAGTSEALLDTNRYLLEHGRANVQQTIYQDDCVILPPVFIHADARVSTSII
                     GPFASIGAGAVIQNSSIRDSILSPGVHITNTHLEGSILGHDVVITGMMGKFNLGDNSW
                     VSW"
     gene            3131..4297
                     /locus_tag="PKL60_07775"
     CDS             3131..4297
                     /locus_tag="PKL60_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNW96070.1"
                     /translation="MTKSVLARRVVELKPSGIRKFFDIAATMQDVISLGIGEPDFTTP
                     APIIRAGIASLERGDTHYTSNHGILPLREAVAQHLNKLYGVSYNPNDEIVLTVGVSEA
                     LYLTFTALLDPGDEVIIPTPCFVSYQAEVQLAGGVAVEVPTSMADNFEPCPEVIEKAI
                     TPRTKAIFIGYPNNPTGAVATRETLSKIAQLAEKYDLMVVSDEIYDRLVYEQEHVCFA
                     ALPGMWERTILLGGFSKAYAMTGWRIGYVAAPAQIASGLVRIHQYSVMSAPTISQYAA
                     LEALRVGEPYVQEMREEYNRRRLLICSGLNAMGLKTSKPGGAFYVFPDITSSGMDDEE
                     FAEKLLYEEHVAVVPGRSFGDSGIGFVRCSYATEYRCIEIALERMAAFLKRHRA"
     gene            4494..5780
                     /locus_tag="PKL60_07780"
     CDS             4494..5780
                     /locus_tag="PKL60_07780"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNW96071.1"
                     /translation="MSGQTNAFEMAQQQFDKVADQLGLDEQVRQILRWPAREFHMRLP
                     VRMDDGKIRVFEAYRVQHNDARGPNKGGLRFHPAETADTVRALSMWMTWKCAVADIPL
                     GGGKGGVVVDPATLSVAEKERLCRAYVRAMYRNIGPRQDVPAPDVGTTPQMMGWMMDE
                     YSRIVGQYTPGVITGKPVGGGGSLGRTEATGFGVIYTVREAMKHLGLTPSECTVALQG
                     FGNVAQYAAVGFKEILGGKVVCVSCWDRDDKVSYTFSKEDGVDPWFLKSITDQYGTIN
                     KEKAREAGYRIEDGSAWISKPVDVLIPAALEGQINAETVRLINPSVKILAEGANGPTT
                     PEADVVLNERGIFIVPDFLCNSGGVTVSYFEGVQNDMNFYWTREEVLDKLNNNMTTAF
                     KGVLDMSLQQNVSMRDAAYMVAIHKVVKAMELRGWI"
BASE COUNT         1235 a         1683 c         1739 g         1175 t
ORIGIN      
        1 ctttcctctt gtccactcag ctcactacac cttgcgctgg cgccacatca caaagtcgta
       61 ggtcgggtgg agcggcagcg caacccgaca agccttggcc ggcaggcctg tgcaacggtt
      121 ttagggttcc gctcctgtgc ccctgcttcc aacctgaatg ctgcctgaag acgcaaaaca
      181 aaagccggct gtacccagcc ggctttcctc ttgtccactc agctcactac accttgcgct
      241 ggcgccacat cacaaagccg agaccaatcc cggcggccaa taccacgatt ccgacgatcc
      301 ctaagattgg agacttgacg ctggctcctg cggcgtcttc gccaggcgcg gccgttgcca
      361 cctgagcgcc aaaatcaatg gtcgcctgct cgccggcttt cacttccagc ggatagttgg
      421 taatcgtcgt ggcgttaaat ccatcgggaa tggccacggt aatgttgtag ctgccctccg
      481 gaatattctc aaaacacagc gggtcgacct cgtccgggtt tccggccact gtggtgccgg
      541 tgaccgaaac cttgcccaag cggtcattga tacttaccac gccgccgtaa aggtaagttt
      601 ccccttccgt gcgcatgccg gtgccgtcca ggtcgttaaa gagcacaatg caaactttgg
      661 caaagcccgg ttcgggggtg ggcgtggggg taatcaattc cggagcaatg gtaggcgagg
      721 gcatggcggt ttgggtcagt tccggggctg ccaccgtccc gagcaggatt ttggtgcccg
      781 tgatgaggtt gcaatcttcg gtcagcccgt tcaagcggat gatatcgtcg atggagacgt
      841 tggtcagcag gaacacgcgc gtgcaggtat cgccttcttc cacggtgtaa taaatgcgcc
      901 cctcggcatc gggtgtgggc gtctggtaca cggcttgcgc gctggccggt cgggaactga
      961 cggcgctcca cagcagaacg ctggaaacga gcattagggc gattatgaca aagggaagga
     1021 gaatcttttt caagctgttc attttcacct ctcattcgga tattaccatt atacccagtt
     1081 atgcggcacg tcaaaactga gcgctgcctg tgagcgctcc gcgcggcggc aggattgggt
     1141 acaataaggc catgaaatcc ttgcgcgcgt gcttcctatg cttgctggcc gcggctttgc
     1201 tgctgggcgc ctgccttccc caacaggctg caccgacacc gggcccaacg ctgcctccga
     1261 cccgcaccgc ccagcccagc ccgaccatcg actggttccc ggacactccc acgccaaccc
     1321 agcgcgttta cgaggtcaca cctaatccat tggaagggct gacgcccccg cagtacgcgc
     1381 aggtcttgta cgaggaggac ttttcgggtg gggagcagtg gaaggaaagc tccggggcgg
     1441 acggtaacat tcgctacggc agcggcgcgc tcgcgctggc actcacaggt aaaaaagcag
     1501 aaatcagcag cctgagcaaa atcatgctgc ccgacagctt ctatctggaa ctgaccctgg
     1561 aatctgcgct gtgcggagcg aacgacagct ttggaatcct gttctggcgc ttcagcgagc
     1621 tgggcacgta ccgcatgctc taccgctgcg atgggcaggc gcgcctggaa cgcgcaatct
     1681 acgacggcgt ctcggtcctg caaaactgga cggcgctgcg gcgcttccag cccaacgcgc
     1741 ccgcgctgaa ccgcctgggc atctgggcgg accagggcac gctttacttt ttcgtgaatg
     1801 acacctttca gttcagcgcg gagggtcgca agggcttgag cggcgctttg ggggtctttg
     1861 cctccgcttc cggggaacag gcagccacct ttaacgtcac cagcctgcgc atcacccagc
     1921 cttaatcccc cggtttggcg cggatacagg tataatcaaa ccaagcctga agggcaaagc
     1981 agtccgccgc aggaatgcac ggactggctg aatactggta tgaggtcggt tttgaaaatg
     2041 caacaagcgc ttactggcag gacaccatcg ctctttctgg agattcccct atgcctgaaa
     2101 cattaaaaat catcatcccc atggcgggtc tgggcagccg gctgcgtccg caaacctgga
     2161 gcaggccgaa accgctgatt tcgctggcag gcagcaccgt gttggaccat atgttgagta
     2221 tgtttaaaac ggtgccggat tccgagcatg tggagtatgt ttttattatg tcccccgggc
     2281 agcgcgagtt gattcacgcg cacatgcaaa agtttcaccc gcgcaagcag gtgcactatg
     2341 tggaacaacc ggaaaagaag ggtcagtccg acgcgctctg gcacgcccgc gaatacctga
     2401 agggtcggct gctggttgtt ttttcggata cgctgattga aaattccttt gcgttcctca
     2461 ataacgaaaa gctcgattcc gtcgcctggg tgaaggcggt tccggacccg cggcgctttg
     2521 gcgtagcgga aaccggcgcg ctggggttgg tgcggaggct gattgaaaag ccaaatgata
     2581 tgaacaacaa tctggcagtg gtcggctgct attacttcaa ggaaggcgag acactgctgg
     2641 cggccgttga agaacaattc cggcgcaaca tctcactcaa aggggagtat tacctggcgg
     2701 acgcgctgaa catttacctg gagcagggag ccgtgatgcg taccgagcag gtcagcgtct
     2761 ggttggacgc gggcacttcc gaagccctgc tggataccaa ccgctatctg ctggaacacg
     2821 gacgcgcgaa cgtccaacag accatctatc aggatgactg tgtgattctc ccgccggttt
     2881 tcattcacgc cgacgcgcgc gtgagcacgt ccatcatcgg tccgttcgct tccatcggag
     2941 cgggcgcggt catccagaac agcagtatcc gcgattccat tctcagcccc ggtgtgcata
     3001 tcaccaacac tcacctggaa ggttccatcc tggggcacga cgtggtcata acgggcatga
     3061 tgggaaagtt taacctggga gataattcct gggtcagctg gtaagcaagc aatagaaaaa
     3121 aggagaattc atgaccaaat ctgtacttgc caggcgggta gttgagttaa aaccctcggg
     3181 gattcgcaaa ttctttgata tcgctgccac catgcaggat gtcatttccc tgggcattgg
     3241 cgaacccgac tttaccacgc cggcgccgat tatccgcgcg gggattgctt ctttggagcg
     3301 cggagacacc cactatacct ccaaccacgg gattttgccg ctgcgcgaag cggtggcgca
     3361 gcacctgaac aagctgtacg gcgtctcata caacccgaat gacgaaatcg tcctcaccgt
     3421 aggcgtatcg gaagcgctgt atctcacctt tacggcgctg ctggaccccg gcgatgaggt
     3481 gatcatcccg acgccctgct ttgtgtccta ccaggccgag gtgcagctgg ccggcggcgt
     3541 ggcggtggaa gtcccgacca gtatggcgga taattttgag ccctgccccg aagtgattga
     3601 aaaggccatc accccgcgca ccaaggctat ttttattggc taccccaaca acccgaccgg
     3661 cgcggtcgcc acgcgggaga cgttgagtaa gatcgcccaa ctggcggaaa aatatgacct
     3721 gatggtcgtt tcggatgaaa tttacgaccg cctggtttac gaacaggagc atgtatgttt
     3781 cgcggcgctg ccagggatgt gggagcgtac catattattg ggcggtttct ccaaagccta
     3841 tgccatgacc ggctggcgca ttggctacgt ggcggccccc gcccaaattg cctccggcct
     3901 ggtgcgcatc caccagtatt cggtcatgtc cgcgccgacc atctcgcagt acgccgcgct
     3961 ggaagcgctg cgcgtaggtg agccgtacgt gcaggaaatg cgcgaggaat acaaccgccg
     4021 gcggctgctc atctgcagcg gcttgaacgc gatgggcttg aaaaccagca aaccgggcgg
     4081 cgctttttac gtcttcccgg atatcacttc cagcgggatg gacgatgaag agtttgcaga
     4141 gaagctgctc tacgaggagc atgtggcagt cgtgccgggg cgctccttcg gggattccgg
     4201 catcggcttt gtgcgctgtt cctatgccac ggagtaccgc tgcatcgaaa ttgcgctgga
     4261 gcgcatggcc gccttcctga aacgccaccg cgcgtgagcc tgacgcggct gtgcttgacg
     4321 caatactttt aatgcagtaa agtaaaagca gcaggctctt gaggaacgaa tgacgcttct
     4381 tccagaagat accaggaaat cattacgggc acccggcgcg gctccggttt gcccgcaatt
     4441 cttttaagag aatgccgcgc ggtattataa aaaatcgatt aacaggagag aaaatgtcag
     4501 gacaaaccaa tgcatttgag atggcacagc agcaatttga caaagttgcc gaccagcttg
     4561 ggctggatga gcaggttcgt cagattctgc gctggcctgc ccgcgaattc catatgcgcc
     4621 tgccagtgcg tatggatgac gggaaaatcc gggtgttcga agcctaccgc gtgcagcaca
     4681 acgacgcccg cgggccgaac aaaggcggtt tgcgctttca cccggcggaa actgccgaca
     4741 ccgtgcgcgc gctttccatg tggatgacct ggaaatgcgc tgtcgccgat atccccctgg
     4801 gcggcggcaa gggcggcgtt gtggtggacc ccgccacgct ttcggtcgcg gaaaaagaac
     4861 gcctgtgccg ggcttatgtg cgtgctatgt accgcaacat cggtccgcgc caggacgtgc
     4921 ccgcgccgga cgttggcacc accccccaga tgatgggctg gatgatggac gaatattcgc
     4981 gcatcgtcgg gcagtacacc cccggcgtaa tcaccggcaa gcctgtaggc ggcggcggtt
     5041 cgctcgggcg gaccgaagcg accggttttg gcgtcattta caccgtgcgc gaggcgatga
     5101 aacacctggg cctcacccct tcggaatgca ctgtcgcgct gcagggcttt ggaaatgtgg
     5161 cgcagtacgc cgcggtcggc ttcaaggaaa tcctgggcgg caaggttgtg tgcgtttcct
     5221 gttgggaccg cgacgacaaa gtttcgtaca ccttcagcaa ggaagacggc gtggacccct
     5281 ggttcctgaa gagcatcact gaccagtacg gcacgattaa caaggaaaaa gcacgcgaag
     5341 ccggataccg gattgaagac ggcagcgcct ggattagcaa gccggtggac gtgctcatcc
     5401 ccgccgcgct ggaaggccag attaacgcgg aaaccgtgcg gctcatcaac cccagcgtga
     5461 agatcctggc ggaaggcgcc aacggaccga ccacccccga agcggacgtg gtgctgaacg
     5521 agcgcggcat tttcatcgtg ccggacttct tgtgcaacag cggcggcgtg accgtctcgt
     5581 actttgaggg cgtgcagaac gacatgaact tctactggac gcgcgaagaa gtgctggaca
     5641 agctgaacaa caacatgacc accgccttca agggcgtgct ggatatgtct ttgcagcaga
     5701 acgtgagcat gcgcgacgcg gcgtatatgg tggccatcca caaggtggtc aaggccatgg
     5761 aactgcgcgg ctggatttag gattttgcta cgttgagatg cgagcagccc gaccggaaga
     5821 tcgggctgct tg
//
LOCUS       DAOQAD010000115         5801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_17191_length_5801_cov_5.982423, whole genome shotgun sequence.
ACCESSION   DAOQAD010000115 DAOQAD010000000
VERSION     DAOQAD010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17191_length_5801_cov_5.982423"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..2617
                     /locus_tag="PKL60_07785"
     CDS             143..2617
                     /locus_tag="PKL60_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNW96072.1"
                     /translation="MLHYLSREQEIQVETWLSQLSLPEKISLLSGADNWSVAAVPRLG
                     IPALIVSDGPHGVRADRASSRRPAGATNAYPTGVGIAATWNRELTRKLGAALAEESRE
                     MGVDVLLGPCVSIMHSPLGGRNFETYSEDPFLAGEIGLNWVLGLQQKGVGASVKHFAG
                     NDQERERMRLNVIISERALREVYLEPFEKIVRRAQPWTVMAAYVRLNGTYASENSWLL
                     RSVLKEEWGFEGAVISDWFATHSAAAALNAGLDLEMPGPALWRGGHLKEAVDTWQVSE
                     DTLDEAVRRVLILIARCRPGQVPPRPGAVDTAQRRALARELARQSITLLKNEGGLLPL
                     PTSAKKIAVIGLNASTRVTGGGSSFVKGPYWVTPLEGLRQALGPEVELVYEPGADNRV
                     LPQVIESSFFSQVDGSAGLLASLYNNPDFEGAPLRKTDPALDSWWGGMGPAEGKINAR
                     CFSGTWEGLYTAAASGETPLFLANNGHAKLFIDDQLVVENHSGDVIPDYGNFSSVLVG
                     GSIGLEAGRQYRLKVEYSFETHAGFPFLQLAHLPPYVPEDGQARAVRAAAEADLAIVF
                     AGNPDGYETEGADRPSMRLPGSQDELIAGVVSANPSTIVVVHAGAPLEMPWAEQVPAI
                     LWAYYPGQEGGNALADLLTGAESPSGKLPMSIPYRLEDDPTFVNYPGDQSLWYGEGIF
                     IGYRSYDLRKMDVRFPFGHGLSYTRFEYSDLYCPESFHPGEKVEISFSLRNSGRQNGA
                     EVAQVYVRDVQSSVARPVRELNGFSRVQLRAGEETRVSIQLDARAFSYYDQDRHCWTM
                     EPGYFEIEVGSSSRDIRLSGSLRLEA"
     gene            complement(2740..3501)
                     /locus_tag="PKL60_07790"
     CDS             complement(2740..3501)
                     /locus_tag="PKL60_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNW96073.1"
                     /translation="MNSETQSNNTTEKRVAILIDGDNAQSSLIEQIVVESARFGNATI
                     RRVYGDWTTSNMKSWKDILNSYAFQPIQQFRYTIGKNATDSAMIIDAMDILHKDQVDG
                     FCLVSSDSDYTRLATRIRESGKMVIGIGKQSTPRAFVSACDVFIYTENLEEREKQARK
                     AAQKQQKMEQLTQADLELIEKVNQAIDMAGSDDDGWSMLSEVGTALRRIDPGFDPRTY
                     GAKQLAQLLRARKTFFDLKKPAGKSLILIRPKEEQ"
     gene            complement(3637..4104)
                     /gene="msrA"
                     /locus_tag="PKL60_07795"
     CDS             complement(3637..4104)
                     /gene="msrA"
                     /locus_tag="PKL60_07795"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNW96074.1"
                     /translation="MRKEAFFAGGCFWGLEARFQCVRGVVETQVGYSGGTTKEPTYRQ
                     VCQDTTGHAETVKVTYETDEISLQQLLELFFSYHDPTNCCSQGLDFGSQYRSMVFYTS
                     ADEKETIEQVIAFLETSGVFDRPVVTQVEMLESFYPAEEYHQNYYRKHGVSVC"
     gene            complement(4112..4522)
                     /gene="msrB"
                     /locus_tag="PKL60_07800"
     CDS             complement(4112..4522)
                     /gene="msrB"
                     /locus_tag="PKL60_07800"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNW96075.1"
                     /translation="MEKIRKADEEWRALLTEEQYRVTRRKGTEAAFCGVFYDHKQDGL
                     YQCVCCGLELFSSDSKFDSGTGWPSFFQPVSAERVEQVLDLSHGMVRQEVRCARCEAH
                     LGHVFRDGPPPTGLRYCLNSAALRFVPEKKQIRD"
     gene            complement(4645..>5801)
                     /locus_tag="PKL60_07805"
     CDS             complement(4645..>5801)
                     /locus_tag="PKL60_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNW96076.1"
                     /translation="AIEEFLVGASPLTRTDFDRDQYSREKILIQCLDAWRLNPRARLI
                     PLYNSPEHHASRRESMHTQYYVGCDLGGTNIKAGLVDMTTGRVIASKDTKTLSYLGHE
                     AVIGRIANLITEMVAEAGFEWSQIGGVGLSAPGRLDIEQGLTKFIPNLPGHWIDVPIK
                     ALLEGYLNVPVYIMNDVRAITYGEWAFGAGKGVDCMLCLAIGTGIGGGVVMNNQLVLN
                     QGGTAGELGHITVDFHGPKCGCGNYGCVETFASGPAIAAMGLKAVVQGQATKIGELVN
                     YDLNKITAHVIATAAEAGDPVALEIWNTAGAYIGVAIMNAALVVGPKRVVVTGGVAAA
                     GELLLRPIREELQRRMLIMPADQVEVVPGELGNDAGIIGLAVWAAHKGDL"
BASE COUNT         1166 a         1729 c         1663 g         1243 t
ORIGIN      
        1 cacagctcca aaacttaatc agggcctttc tattttgcta tttcaggaca gtatgccttt
       61 gcgcattttc ccctcccagc cgtatattgg acagtcggac aggtataata caaacagctc
      121 acagcgcact ctggaggtca gcatgcttca ttatctttcc cgtgaacaag aaatccaggt
      181 ggaaacctgg ctcagccagc tcagcctgcc cgaaaagatc agcctgcttt ccggcgcgga
      241 taactggtcc gttgccgctg taccccgcct gggcatcccc gcgctgattg tcagcgacgg
      301 accgcacggt gtgcgcgctg accgtgccag cagccgccgc cctgccggcg ccaccaacgc
      361 ctacccgacc ggggtcggta ttgccgccac ctggaaccgc gagctcaccc gcaagcttgg
      421 cgcagctctg gctgaggaat cccgcgaaat gggcgtggac gtccttctgg gcccctgcgt
      481 cagtatcatg cacagccccc tcggagggcg caactttgaa acctattccg aagacccatt
      541 cctggcgggg gaaatcggcc tgaattgggt tctgggactg cagcagaagg gtgtgggcgc
      601 ttccgttaag catttcgccg gcaatgacca ggaacgcgag cgcatgcgtc tgaatgtgat
      661 tatctccgaa cgcgcgctgc gcgaggttta cctggagccc tttgagaaaa tcgtgcgccg
      721 cgcccaaccc tggacggtca tggcagcgta cgtccgtctg aacggcacct atgcctcgga
      781 aaactcctgg ctcttacgtt ctgtgctgaa ggaagagtgg ggctttgaag gcgcggtcat
      841 ctcggattgg ttcgcgaccc actccgccgc tgccgcgctg aacgccgggc tggacctgga
      901 aatgcccgga ccagcgctct ggcgcggagg ccacctgaaa gaagccgtgg atacctggca
      961 ggtatcggaa gacacgctgg atgaggctgt gcgtcgggtg ctcattttga ttgcgcgctg
     1021 ccggcccggt caggttccgc ccaggcccgg cgcggtggat accgcccaac gccgcgcgct
     1081 ggcaagggag ctcgcgcggc agtccatcac gctgctgaag aacgagggcg gcctcttgcc
     1141 gctgccaaca agcgcgaaaa agattgccgt gatcgggctg aacgccagca cccgcgtaac
     1201 tggcggaggc agctcctttg tgaaaggacc ttattgggta accccgctgg aaggtttgcg
     1261 ccaggcgctc ggtccggagg tggagcttgt atacgagccc ggcgcagata accgtgtact
     1321 cccccaggta attgaaagca gcttcttctc gcaagtggac ggcagcgctg gattactggc
     1381 aagcctgtat aacaatccgg attttgaggg cgcgcccctg cgaaagactg accctgcctt
     1441 ggattcgtgg tggggcggca tgggacccgc ggaagggaaa attaacgcgc gctgcttcag
     1501 cggcacctgg gaaggccttt acactgccgc cgcaagcggc gaaactccgc tctttctggc
     1561 caataacgga cacgcgaaac tattcatcga cgaccagctt gtcgtggaaa accacagcgg
     1621 agacgtcatc ccggattacg gcaacttcag ttccgtgctc gtcggcggtt cgatcggcct
     1681 ggaagccggc aggcagtacc gcctgaaagt ggaatactcc ttcgagactc acgctggttt
     1741 cccgttcctg cagctggcgc acctgccccc ttacgtcccg gaagacgggc aggctcgggc
     1801 tgtgcgggct gccgcggaag ccgacctggc gattgttttc gccggcaatc cggacggata
     1861 cgaaacggaa ggcgcggacc gcccgtctat gcgcttgccg ggcagtcagg atgaattgat
     1921 tgccggggtc gtcagcgcca accccagcac cattgtcgtg gttcacgccg gcgccccgct
     1981 tgaaatgccc tgggcggaac aggtccccgc gattctatgg gcttactacc cgggtcagga
     2041 aggcggcaac gcactcgcgg acctgctgac aggcgcggaa tccccctccg gaaagcttcc
     2101 gatgagcatc ccctacagat tggaagacga cccgaccttt gtcaattacc cgggcgacca
     2161 atccctttgg tacggagaag gcatttttat cggctaccgc agctacgacc tgcgaaagat
     2221 ggatgtgcgc ttcccattcg ggcatgggct ttcctatacc cgttttgaat acagcgacct
     2281 atattgccct gagagttttc atccgggtga aaaagtcgaa atcagctttt cgctccgcaa
     2341 cagcgggagg caaaatggcg cggaggtcgc gcaagtctat gtgcgggatg tgcaatccag
     2401 cgtggcgcgg cctgtgcggg agctgaacgg cttcagccgc gtccaactga gagccggcga
     2461 ggaaacccgg gtgagcatcc aactggacgc gcgcgccttc agttactacg accaggacag
     2521 gcactgctgg acgatggaac cgggctactt tgaaattgaa gtgggcagtt cctcgcgcga
     2581 tatccgcctg agtggcagcc tgcgattgga ggcttaagca tctcttccat taccatgtag
     2641 catgcaaggg ctgcttcctg cccccccaga aagctgtttt cagaaggaaa caagacactc
     2701 tacaaaaaag agttcccgta tggagctctt tttcgtgatt tactgttctt ctttcggtcg
     2761 gatcaaaatc aaggatttcc cggctggctt tttcaggtcg aagaaggttt tgcgggcgcg
     2821 cagcagctgg gcaagctgtt tggcgccgta ggttcgcggg tcaaaacccg ggtcgatccg
     2881 acgcaaagcg gtgccaacct ccgaaagcat tgaccagccg tcgtcatccg agccagccat
     2941 atcaatcgcc tgattgacct tctctatcag ttcaagatcg gcctgggtca actgctccat
     3001 tttctgttgt ttctgggcag ccttgcgggc ctgtttctcg cgttcttcca ggttttcggt
     3061 gtatatgaac acatcgcagg cggagacaaa ggcccgcgga gtggactgtt tcccaatccc
     3121 gatgaccatt ttgccacttt cgcggatgcg cgtggccaga cgggtgtaat cgctgtcgct
     3181 ggagaccagg caaaaaccgt ccacctggtc tttgtgcagg atgtccatcg cgtcgataat
     3241 catggcggag tcggtggcgt tcttcccaat ggtgtagcgg aattgctgga tgggttgaaa
     3301 tgcgtacgaa ttcaggatgt ccttccagga cttcatgttc gaggtggtcc aatccccata
     3361 gacccggcgg atggttgcgt tgccaaagcg ggccgattcc accacgatct gctcaattaa
     3421 gctggactgc gcgttatcgc cgtcgatcag gatagcgacg cgcttttcag tagtgttgtt
     3481 gctttgtgtt tcagagttca tacaaccatt ataccctgta tacccctgcc ccgctgagcg
     3541 ttctcggccg ggaaattaag ctgactgcgc tctcaactcc ccgggttttc tcgtgaatgt
     3601 ctatgaaaaa gccgcggtct ctgccgcggc tctggattaa caaacgctga ccccatgttt
     3661 ccggtaatag ttctggtggt attcctccgc cgggtaaaag ctctccagca tttccacctg
     3721 cgttaccacc ggccggtcaa atacgccgga ggtttccagg aaggcgatga cctgttcaat
     3781 cgtttctttt tcatccgcgg aagtgtaaaa caccatcgaa cgatactgcg agccaaaatc
     3841 cagaccctgc gagcagcagt tagtcgggtc gtgataggag aagaagagtt ccaggagttg
     3901 ttgaagactg atctcgtccg tctcgtaggt tactttcacg gtttcggcgt gaccggtagt
     3961 atcctggcaa acctgacggt aggtcggttc ttttgttgtg ccgccggaat acccgacctg
     4021 ggtttccacc acgccgcgca cgcactgaaa cctggcttcc agtccccaaa aacatccccc
     4081 cgcgaaaaat gcttccttcc tcatcagctt cttaatccct tatttgtttt ttttctggca
     4141 caaagcgcaa ggctgccgag ttcaaacaat aacgcaaacc tgtaggcggc ggtccgtccc
     4201 ggaagacatg ccccaggtgg gcctcacagc gcgcgcagcg cacttcctgg cgcaccatcc
     4261 cgtggctgag atccagtacc tgttcgacgc gttctgccga tacaggttgg aaaaaagacg
     4321 gccagccggt tcccgaatcg aacttgctat cggaagaaaa cagctccaga ccgcagcaga
     4381 cacactggta gaggccgtcc tgcttgtggt cgtagaacac gccgcagaaa gcggcttccg
     4441 tgcccttccg gcgtgtcacg cggtactgct cttcggttag cagagcgcgc cattcttcat
     4501 cggctttgcg aatcttttcc ataacaatct gttgtgaata taacacggaa aaaggagctc
     4561 aaacgctcct gcacatccct agtacagagt tgttaacata aaaagctgcc atttctggca
     4621 acttgtgaaa tattgaacag aggcttacag gtcgccctta tgcgccgccc agacagccag
     4681 cccgataatg ccggcgtcgt tcccgagttc acccggcacg acttccacct ggtcagccgg
     4741 cataatcagc atccgccgct gcagctcctc gcggatcggg cgcaggagca gttcgccggc
     4801 agcggccacg ccgcccgtca cgaccacgcg cttgggccca accaccagcg ctgcgttcat
     4861 aatcgccacg ccaatatatg cgccggcggt attccagatt tccaatgcca ccgggtcgcc
     4921 ggcctccgcc gcggtcgcga tgacatgcgc ggtgatttta ttcaggtcgt aattcaccag
     4981 ctcgccaatt ttggtcgcct gcccctgcac gaccgctttc agccccatcg ccgcgatggc
     5041 tgggccggac gcgaaggttt ccacgcagcc gtaattgccg cagccgcact tgggaccgtg
     5101 aaaatccacc gtgatgtgcc ccagttctcc ggctgtgccg ccctggttca ggacgagctg
     5161 gttgttcatt accaccccgc cgccaatgcc ggtgccgatg gccaggcaaa gcatgcagtc
     5221 cacgcctttg ccggcgccaa aggcccattc gccataggta atcgcgcgca cgtcgttcat
     5281 gatatacacc ggcacattca agtagccttc cagcagcgct ttaatcggca cgtcaatcca
     5341 gtgcccgggc aggttgggga taaatttagt gagcccctgc tcgatatcca gccggccggg
     5401 ggcggatagg cccacgccgc caatctggga ccattcaaag cctgcttccg cgaccatctc
     5461 ggtaatcaag ttcgcgatgc ggccgatgac cgcctcgtgc ccgaggtacg agagcgtttt
     5521 tgtgtctttg gaggcgatca cccggccggt ggtcatatcc accagtccgg ctttgatatt
     5581 cgtgccgcct aaatcgcaac cgacgtagta ttgtgtgtgc atgctttctc tcctgcttgc
     5641 atggtgttcg ggtgaattat acaacgggat gaggcgggcg cgggggttga ggcgccaggc
     5701 gtcgaggcat tggatgagga ttttttcgcg ggagtactgg tcacggtcga aatcggtgcg
     5761 ggtgaggggg gaggcgccga cgaggaattc ctcgatcgcg c
//
LOCUS       DAOQAD010000116         5799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_17200_length_5799_cov_6.809889, whole genome shotgun sequence.
ACCESSION   DAOQAD010000116 DAOQAD010000000
VERSION     DAOQAD010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17200_length_5799_cov_6.809889"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKL60_07810"
     CDS             complement(<1..626)
                     /locus_tag="PKL60_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96077.1"
                     /translation="MSNSNKKNCHILVPILLTLMLAGCQAGTAVPLPSETAALPTAAP
                     RLPTPTATSTPTQTPTATAVPIPAAELADLNGLELTVLYPWINESEAAFNSLVENFNT
                     NNAWGLRVVPGSGGSLSAVADGLAAGELDANLVIGNGFDLAPAAKSGTLISLTPYMRD
                     AEWGLSGVYPPEFVFADLAPNPDDETDWGYLPIAYQPALIYYNQSWGE"
     gene            complement(647..740)
                     /locus_tag="PKL60_07815"
     tRNA            complement(647..740)
                     /locus_tag="PKL60_07815"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(704..706),aa:Sec,seq:tca)
     gene            882..2333
                     /locus_tag="PKL60_07820"
     CDS             882..2333
                     /locus_tag="PKL60_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNW96078.1"
                     /translation="MTAELNHTPDWVKNAVFYQIFPDRFAWSPRVEKPNNFESWDSAP
                     TAYGFKGGDLLGVAEKLDYLEDLGINAIYFNPIFQSASNHRYHTHDYFQVDPLLGGNK
                     AFKELLTEAHKRGIRVILDGVFNHSSRGFFQFNHILEVGEKSPYLDWFFVHGFPLHAY
                     EGSPNYDCWWSLPGLPKFNTNNPQVRKYILSIARHWLEEGIDGWRLDVPFEIDDDDFW
                     REFREVVKTANPDAYIVGEIPTEAQRWMRGDMFDAVMNYQLTRACLAFFGGSKMDSVL
                     AEGMMGFNVPEPMDAAQFARRTRELLRIYPQEFAYAQLNLLDSHDMPRFLSLVSEDTQ
                     RLYLAYLFMMTYPGAPCIYYGDEIGLTGGIDPMCRKSFPWDESAWNTGLRDKIRELTL
                     LRHAMPVLRTGEYLPLLTEGSLLVYARKDSEDAVIVAINLENSPQTVTLRVDSLLAEE
                     SLLQDQLSEKQVEVKAGEIRDLTIPRMSALILA"
     gene            complement(2387..3043)
                     /locus_tag="PKL60_07825"
     CDS             complement(2387..3043)
                     /locus_tag="PKL60_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNW96079.1"
                     /translation="MIKSSFESAPMVNTDMSEQQKLSQPENQETPMESGGSFIQRLLK
                     LDARLSAWLVVEEKPGLLRSLYIFLGHSCDSWYWLIFLSLLLFFGSVEVRARTLFWMV
                     GLIGLAVFVLALKFIIRRPRPEGEWGQIYRVSDPHSFPSGHAARAAGIAVMVSTAAAP
                     GLIAGMAAWALGVGLSRVALKLHYLSDVVVGWIIGILCGLAAARFFPWIEPWLLGLLG
                     "
     gene            3178..3855
                     /locus_tag="PKL60_07830"
     CDS             3178..3855
                     /locus_tag="PKL60_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNW96080.1"
                     /translation="METIHLAVLWDMDGTILDTLDLHYLTWKEAFSDRPTRFSRELFR
                     ANFGGNNRSTVPIYLGYEPDEAYYQHVVALKENLFREQMAEKTQLFPGILDWFAYFAR
                     LGAKQAIASSAMLENITKVVDAFGIRKYFDALVPGEDLPSKPAPDIFLRAAQLLGAPP
                     ARCVVFEDSIHGINAAKAAGMVSVGVAGTTRFAPGDADFLLDDYTQDPAALTSKILAL
                     MDSRLPA"
     gene            complement(3852..4619)
                     /locus_tag="PKL60_07835"
     CDS             complement(3852..4619)
                     /locus_tag="PKL60_07835"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNW96081.1"
                     /translation="MSDQTPLENQTPPEAPAQTRRVALELNKPIVTYVLMGVIALFFI
                     AQMLSTQFLGYDLPAALLSKSSSAILAGQLWRLITPVLLHGSILHVGLNLYALWILGR
                     QLEGIYGHWRFLILFLIAGFGGNVLSFVLSPYNSLGSSTGIFGLLAAEILLILQNRQF
                     FGPRARAMLSNLVFIFFLNIAIGLAPGSNIDNFGHLGGAIAGGLFAFLAGPKWELRFS
                     QTAPRVQDTRQKNDVVIAVLIVAVGFALIAAIPFLQF"
     gene            complement(4616..5242)
                     /gene="gmk"
                     /locus_tag="PKL60_07840"
     CDS             complement(4616..5242)
                     /gene="gmk"
                     /locus_tag="PKL60_07840"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNW96082.1"
                     /translation="MTPPVFHFGPEPFTPLLIVISGPSGIGKDAVVQALRQENPSTHF
                     VVTMTSRPPRPEERDGVDYFFVSRQEFESLIASGELLEHARVYSDYKGIPREQVRRAL
                     QSGKDVIMRLDVQGAMTIRSLCPEAVLIFLTANSREEWIARLAGRCSESPEECMLRIQ
                     TAEQEYEKLDAFDYIVVNADCQLHETVDAIQKIISAEHHRVHPRQVCL"
     gene            5518..>5799
                     /locus_tag="PKL60_07845"
     CDS             5518..>5799
                     /locus_tag="PKL60_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNW96083.1"
                     /translation="MAKAVEINGSPTLDKGRTAFMLAPFMQGMRSAGAEISLYYASKM
                     DVPTCTCGKMACWNIHPGVCVFQDEMDALLEALKSAEFLVFGVPVFIQLP"
BASE COUNT         1298 a         1701 c         1615 g         1185 t
ORIGIN      
        1 tcctcgcccc agctctggtt gtagtaaatc agggcgggct gataggcgat cggcaggtag
       61 ccccagtccg tttcatcgtc cggattagga gccaggtctg caaagacgaa ctccggcggg
      121 taaaccccgc tcagacccca ttcagcgtcg cgcatgtatg gagtgaggga aatcagcgtg
      181 cccgatttcg ccgcgggcgc gaggtcaaag ccgtttccga taaccaggtt ggcatccagt
      241 tcgcccgcgg ccagtccatc cgcaaccgcg gataagctgc cgcccgaacc cgggacgacg
      301 cgcagccccc aggcgttgtt ggtattgaaa ttttctacca ggctattgaa ggctgcttca
      361 gactcattaa tccatgggta cagcacagtt aattccagtc cgttcaggtc tgccagttcg
      421 gcggcaggga ttggcaccgc cgtggcggtg ggcgtttggg tcggcgtgct ggtggcagtc
      481 ggcgtgggga gcctcggcgc ggcggtcggg agcgcagccg tctcggaagg cagcggcacc
      541 gccgtgccag cctggcagcc tgccagcata agcgtcaaaa gtatcggtac caggatatgg
      601 caattttttt tattagaatt cgacacagtg caccttaaat ttcaggcggg aatggatggg
      661 agtcgaaccc accacggctc gcagcgcgac ccgacactga ttttgaagac caggaagccc
      721 accgggacct aaccattccc acggctaagc atatccgaaa tggaagaggc ttgtcaagct
      781 gcccgttccg gtttgaaatg ccagaatccg taactggcag ccgcattttc gcggcagtct
      841 attgtataat gtgcatccga ctcaaattac agaggaaatc gatgaccgca gaattaaacc
      901 acacccctga ttgggtaaaa aatgccgttt tttatcaaat cttccccgac cgcttcgcct
      961 ggagccccag ggtggaaaaa ccgaataact ttgaatcctg ggacagcgcc ccgaccgcgt
     1021 acggctttaa aggcggggac ctgttaggcg tggcggagaa attggattat ctggaagacc
     1081 tgggtatcaa cgccatctac ttcaacccga tttttcaatc cgccagcaac caccgctacc
     1141 acacccacga ttatttccag gttgacccgc ttttgggggg gaacaaagcc ttcaaagaac
     1201 tgctgaccga agcgcataag cgcggtatcc gcgtcatttt ggacggcgtt ttcaatcatt
     1261 ccagccgcgg cttctttcag ttcaaccata tcctggaagt cggagagaag tccccttacc
     1321 tggattggtt ctttgtgcac ggcttcccgc tgcatgccta tgaaggcagc ccgaattatg
     1381 actgctggtg gtcgctgccc gggctgccca agttcaacac caataacccg caggtgcgca
     1441 aatatatcct cagcatcgcc cgccattggc tggaggaagg cattgacggc tggcgcctgg
     1501 acgtcccctt tgaaattgac gacgacgact tctggcggga attccgcgag gtagtcaaga
     1561 ccgccaaccc ggacgcttac atcgtcggcg aaatcccgac agaagctcag cgctggatgc
     1621 gcggcgatat gttcgacgcg gtgatgaatt atcagctgac gcgcgcctgc ctggcttttt
     1681 ttggcggcag caaaatggac agcgtgctgg cggaaggcat gatgggtttc aatgtaccgg
     1741 agccgatgga cgcagcccag ttcgccaggc gcacccggga actgctcaga atctacccgc
     1801 aggaattcgc ctacgcgcag ctcaacctgc tcgacagcca cgatatgccg cgcttcctga
     1861 gcctcgtcag cgaggacacc cagcgcctct acctggctta tctcttcatg atgacctacc
     1921 cgggcgcccc gtgcatttac tacggcgacg aaatcggcct gaccggcggc atcgacccga
     1981 tgtgccggaa gtccttcccg tgggatgaaa gcgcctggaa cacaggcctg cgcgataaaa
     2041 tccgcgagct gaccctttta cggcatgcca tgcccgttct gcgcacgggc gagtatctgc
     2101 ccctgctcac cgagggttcg cttctggtct acgcgcgcaa ggacagcgaa gacgctgtca
     2161 tcgtcgcgat taacctggag aacagccctc aaaccgtcac tttgcgcgtg gacagtttgc
     2221 tggcggagga gagcctgctg caagaccagc tcagcgagaa acaagtggaa gtgaaagccg
     2281 gcgaaatccg ggacctgacg atccccagga tgagcgcgct cattctggcc tgacttcttg
     2341 cataggaaaa cgaagcgcgc ggagattaaa ctctccgcgc gtttttttat cccagcaaac
     2401 ccagcagcca gggttcgatc catggaaaga accgcgccgc tgccaaaccg cataagatgc
     2461 cgatgatcca accgaccacc acatccgaga ggtaatgcag cttcaacgct acgcgggaaa
     2521 gccccacgcc caacgcccag gcggccatcc cggcaatcaa ccccggagca gccgccgtgc
     2581 tgaccatcac cgcgatgccg gccgcgcggg cggcgtgccc ggacgggaag gaatgcgggt
     2641 cgctgacccg gtaaatctgc ccccattccc cttccgggcg cgggcggcga atgatgaact
     2701 ttagcgccaa cacgaatacc gccaatccga tcaaaccaac catccagaac agggttcgcg
     2761 cacgcacttc cacgcttccg aagaaaagca gcaagctgag aaaaatcaac cagtaccatg
     2821 aatcgcagct gtgccccaaa aagatgtaca gcgagcgcag caaaccaggc ttctcttcca
     2881 cgaccagcca ggcggaaagg cgcgcgtcca gcttcagcaa acgctggatg aaggaccccc
     2941 cgctttccat aggggtttcc tggttttctg gttgggatag tttctgctgt tcggacatat
     3001 ctgtattgac cattggcgcg gattcaaatg aggattttat catacgctcc atctcaccgg
     3061 ataataacgt ttatccccgg attccgcctg cccggctacc cctcaaacgc gtccctttga
     3121 ccagacgcgc actttgggta tgttatgatt acgcaactat attgcttgag gtgcgcattg
     3181 gaaacgattc atctggctgt tttgtgggac atggatggca ccatcctgga cactcttgac
     3241 ttgcattacc tgacctggaa agaagccttc agcgaccgcc ccacacgttt ttcccgcgaa
     3301 cttttccgcg ctaactttgg tgggaacaac cgcagcaccg tcccgattta tctgggttac
     3361 gagcccgatg aagcctatta ccagcatgtg gtcgcgttaa aagaaaacct gttccgggag
     3421 caaatggctg aaaaaacgca gctcttcccc ggcatccttg attggtttgc ctactttgcc
     3481 aggctcggcg cgaagcaagc catcgcctcc tccgctatgc tggagaacat cacgaaagtg
     3541 gtggacgcgt tcggtattcg gaaatacttt gatgctttgg tgccggggga agacttgccc
     3601 tccaaacccg ccccggatat tttcctgcgc gccgcccaat tactgggcgc tcctcccgcc
     3661 cgctgcgtgg tcttcgaaga ctccattcac gggattaatg ccgccaaagc tgccggcatg
     3721 gtcagcgttg gtgtggcagg caccacccgt tttgcccccg gggatgcgga ctttctcttg
     3781 gatgactata cgcaagaccc cgccgccctc accagcaaaa tcctggcctt gatggactcc
     3841 cgcctgcccg cttaaaactg aagaaaagga attgccgcaa tcagagcgaa tccgacggcg
     3901 acaatcagca cagcgatgac cacatcgttt ttctggcgcg tgtcctgcac gcgcggggcg
     3961 gtctggctga accgcagctc ccatttggga cctgccaaaa acgcgaacaa gccgccggca
     4021 atcgcgccgc ccagatgccc aaagttatca atattcgagc cgggagcaag cccgatggcg
     4081 atgttcagga agaagataaa caccaggttg ctcagcatcg cgcgggcgcg cgggccaaaa
     4141 aactgccggt tttgcaggat gagcaagatc tccgccgcga gcagcccgaa aatgcccgtg
     4201 gaagagccca gagagttgta cggtgaaagc acaaatgaaa gcacgttccc gccaaacccc
     4261 gcaatcagga acaaaatcag gaagcgccaa tgcccgtaaa tgccttccaa ctgtcgtccc
     4321 agaatccaca gcgcgtatag gtttaaaccg acgtgcagga tggagccgtg cagcaggacg
     4381 ggcgtgatta gccgccaaag ctgacctgcc agaatggcgc tgctgctttt gctgagcaag
     4441 gcggccggca ggtcatatcc caggaactgc gtcgaaagca tctgcgcgat aaaaaacagg
     4501 gcaatcaccc ccatcaagac ataagttacg atgggtttat tcagctcaag cgccaccctg
     4561 cgcgtctgcg ccggggcctc aggcggcgtc tggttttcca gcggggtttg gtcgctcaca
     4621 ggcacacctg ccgggggtgc acgcggtggt gttccgcgct gatgatcttt tggatggcgt
     4681 ccacggtctc gtgcagttga cagtccgcgt tcaccacgat gtaatcgaag gcgtccagct
     4741 tttcgtattc ctgctctgcg gtctgaatgc gcagcatgca ttcctcaggg gattcgctgc
     4801 agcgccctgc caggcgcgcg atccattcct cccgcgagtt ggcagtcaga aaaatcagca
     4861 cagcctcagg gcatagggag cggatggtca tggcgccctg cacgtccagg cgcataatta
     4921 catccttgcc ggactgcaga gcccggcgca cctgttcgcg cgggatgcct ttgtagtccg
     4981 agtatacccg cgcgtgttcc agcagctcgc cagaggctat caagctttca aattcctggc
     5041 gggaaacaaa gaaataatcc acgccgtcgc gttcttccgg gcgcggaggg cggctggtca
     5101 tggtgaccac gaaatgcgtg ctggggtttt cctgccgcag ggcctgtacc acggcgtcct
     5161 tcccaatccc ggaagggccg gaaatgacga tgagcagcgg cgtaaagggt tcagggccaa
     5221 aatgaaaaac aggtggagtc atggattttc ccgtattctg cgaatttgtg ccttgattat
     5281 aagccatctg gcctgaatgc aaagaaaacc tgtagacagg aaggtgtccg atttggggtt
     5341 ttcctgattg cgggctatac aaaatttaga gggcaggcca gtttctacag cgcgttcaaa
     5401 accagtctgc ccatcgctgc ttgcgccgcg cgtcggttta tcattcttga gagtaatttc
     5461 ctggggttcg cgaccgcgag agcagcgcgt tgaaagcgtt cagaaaaagg agtctacatg
     5521 gcaaaagcag tcgagattaa tggcagccca accttggata agggtagaac tgccttcatg
     5581 ctcgccccgt tcatgcaagg catgcgctcg gcaggcgcag aaatctcgct ttattatgcc
     5641 agcaaaatgg atgtgcctac ctgcacctgc gggaagatgg cttgctggaa tatacacccc
     5701 ggcgtgtgtg ttttccagga tgagatggac gcccttttag aagcgctgaa gtccgcggaa
     5761 tttctcgttt ttggggtgcc ggtattcatc cagctccct
//
LOCUS       DAOQAD010000117         5714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_17581_length_5714_cov_4.730164, whole genome shotgun sequence.
ACCESSION   DAOQAD010000117 DAOQAD010000000
VERSION     DAOQAD010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17581_length_5714_cov_4.730164"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKL60_07850"
     CDS             <1..297
                     /locus_tag="PKL60_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNW96084.1"
                     /translation="VGPGVPVYEGMLVGETPRPQDLSINVAKKKHLTNMRQSIRDLED
                     RLNPPRVLSLDEMIEYIGPDELLEVTPQSLRMRKRILQTHDRLRYEKTGEEEED"
     gene            complement(316..1191)
                     /locus_tag="PKL60_07855"
     CDS             complement(316..1191)
                     /locus_tag="PKL60_07855"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNW96085.1"
                     /translation="MARIRFISQSILQVGNILGECPLWHPQEHALYWLDIDAGLLQRY
                     RPALKQLEVFDLGKRAGSFGFRKDGGLVIATESGFAFWSERDGLSEDFAPVYPQGSPN
                     MMNDGRVDSQGRFWAGSKGPVGSSSLFCLAAHGKYTRVLDKLTIANGIDWSPDGKYCY
                     FTDSGEQAIYRFTIKEGQLRAKEVIFSTIGRGEPGTPDGLCVDAAGNIWSAIWDGSKV
                     VQLSREGETLQEIYLPISRPTSLTFGGSDLQDLYITSASVELSPEQLSNQPQAGDVFC
                     CRVEIPGRPPHFYAG"
     gene            1371..3878
                     /locus_tag="PKL60_07860"
     CDS             1371..3878
                     /locus_tag="PKL60_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96086.1"
                     /translation="MRSAIKKPHFFTACLLFLTGTEGLLFLGLSLPKFQDLLSSNPRA
                     AALLAAWAAGALSAAILGIAAFCGHAGAQSVIEKLKRAFSSKKKWSVWFVPAGLSVYL
                     LASTLMIRIWGANNPFLIRLAEPLGRWFFLSVLQALLVFFFISEQKFSRGYKRTAAFI
                     LAAIALVWLLVLSTRIGLEPDNRYWNVAGVPVLLNQLGLILFLALFGIFLYGLLTRKP
                     AKNPRRREILLDLLACLILWIGAAWFWHQAPFSNSFFAEGKFPPNQDFYPYSDAALTD
                     LGGQYMLIGKGLEYPYFTEKPLYALFLGLLHKFAGQSYLTVSSWQMICFAVLPVLLYL
                     LGKQLHDRLFGLSLALFSVAKEYNAIFSTFKISVSNSRLYLSEFPTLIFMVALAIALV
                     AWFKHSGKKNPWPLLAGGALGLAAMVRTNPLFLIPVILLFGFWIYRRNLKRYWGSALL
                     FLCGILLVIGPWLAYNRIKYGTDPFSYKISAVIQTRFQQQVQQPAPTGLDQHDISLLG
                     EQPVIPSDTQPDEGAPMSARLGAAGFTLGHFLNNEIKALFTLPFTVYPLEITPVLDLP
                     YWEEPVSWRGEMPLSTVLAFALNLALIGIGIAASWKHNSAAGLVPLILNIGYYASNAL
                     GRTSGSRYLLPVDWTLYFYFLLALVLLYESGTHLRSTLPEEQKPALDQLSGQKRRPLP
                     WTVSAGLLLILGASIPIINLSFPELYTHQVQSETKNALLSSPLFNSNKDLTAQSELLV
                     KKRSAQLFKGRMLYPRYRDFVLNGKEGLILTVLRPELTEVFFSFDTDDLTYLEAGQEI
                     IVLGCQREGYVEAFTAYFPEQGLTMRSNNPDFISSCP"
     gene            3984..4793
                     /locus_tag="PKL60_07865"
     CDS             3984..4793
                     /locus_tag="PKL60_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96087.1"
                     /translation="MNADKQTKTPPEDYVLRLFSRKSLFPWLLVLACMLAGALLGALL
                     SLALPPVYEARALVSTNLELVQDANITEIMLDAEINHIGELVFHPEVVNALIEKEAVQ
                     GNMLTLEQLKDSTSVERQLMNTVIKVRSPDPELAARIASEWAEILFTRLNEAYTHAVE
                     LSSAKMQYNSIRDCLTAQPKAPKSFCATLTADNTRADLERLTQIIIAESPQTLGLTVA
                     LNVSQYQPAALPGEPLYYSRGALILGGALAGLAAGFALAELTPGLKRNDEA"
     gene            4783..>5714
                     /locus_tag="PKL60_07870"
     CDS             4783..>5714
                     /locus_tag="PKL60_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96088.1"
                     /translation="MKPKLSARLKGVFWALLLAALPFTSFPLISRLSGGTSVAPLSAV
                     FLLILALFFFLPDFFRRPAVPRQALPLLLFFLSALASSALAVFLPFESFRGAAVWRNA
                     LEGILTIGLGIAFYLVTISVVSDKQALKTTLKWIYLGGALAILASLAQAACWYALGTY
                     PAVLKNFQALISSSGILYKARVTGLAFEPSWLANQLNMLYLPLWIGLSVKGYSVFRRK
                     LFGLFSVENILMGMGLLVLFLSFSRIGWLTTLAMAAFLVFRAANKLMNKYLDKRSLRT
                     GRESSKLRHFFAKLGLWMSLLLVFVLILLLLGVFL"
BASE COUNT         1205 a         1576 c         1542 g         1391 t
ORIGIN      
        1 gtgggaccgg gcgttccggt ctatgaaggc atgctggtag gcgagactcc tcgccctcag
       61 gatttatcca taaacgtggc aaaaaagaag cacctgacca acatgcgcca gtccatccgg
      121 gacctggaag atcgattaaa cccgccgcgc gtgctcagtc tggatgaaat gattgaatac
      181 atcggaccgg acgaactgct ggaagtgaca ccccaaagcc tgcgcatgcg caaacgcatt
      241 ctgcagaccc acgacaggct gcgctatgag aaaaccggcg aagaagagga agattagttc
      301 cgcgcagggt tgatttcagc cggcatagaa atgcggcggt ctgcccggta tttccacgcg
      361 gcagcaaaaa acgtcgcctg cctggggctg gttagaaagc tgttcgggac tcaattctac
      421 agaagctgag gtaatgtata agtcctgcag gtccgacccg ccaaatgtga gcgaagtcgg
      481 cctgctgatt ggcagataga tttcctgcag ggtttcccct tcgcgcgaaa gctggacgac
      541 tttggagccg tcccaaatgg cggaccaaat attcccggcg gcatccacgc ataatccgtc
      601 gggggtgcca ggctcaccgc gtcctatggt ggaaaaaata acttcctttg cgcgtagctg
      661 tccttccttt atggtgaagc ggtagattgc ctgctcgcct gagtcggtga agtaacaata
      721 cttaccgtcc gggctccaat cgatgccgtt cgcgatggtt aatttgtcca gaacccgggt
      781 gtactttcca tgggctgcca ggcaaaagag ggaggaagaa ccgaccgggc ctttgctgcc
      841 tgcccagaaa cggccctggc tgtccacgcg gccgtcgttc atcatattcg ggctgccttg
      901 ggggtaaaca ggcgcgaaat cctcagaaag accgtcccgt tcggaccaaa aagcaaaacc
      961 ggattcggtt gcgattacca atccgccgtc ttttcgaaag ccaaaactgc cggcgcgttt
     1021 cccgaggtcg aaaacttcaa gctgtttgag cgcaggcctg tagcgctgca gtaatcccgc
     1081 gtcgatatcc agccagtaca gagcgtgctc ctgtggatgc caaagcgggc attcccccag
     1141 gatgttgccg acctgcagga ttgattgaga aatgaaacga atgcgcgcca tagttcctcc
     1201 aagtcaattg taacaaaagc gcaccagcct gcccgaattg agctgattat cagcagcttc
     1261 gaactgagac aacgaacccg cagctttctg agttggtcca caaagcgcag actccatcct
     1321 gccgtataat tacggcactg acgagcgaaa atttgctgag gatgccggga atgcgctctg
     1381 cgatcaaaaa accacacttt ttcactgctt gcctgctttt tctgacaggt acagaagggc
     1441 tgctcttcct gggcctttct ttgccgaaat ttcaggattt gctcagctca aacccccggg
     1501 ctgcagcgct tcttgccgct tgggcggcag gcgcgctgag cgcggcaatc ctcggcatcg
     1561 cggcgttttg tggtcacgcc ggcgcgcaga gcgtgatcga aaaactaaag agggctttct
     1621 catccaaaaa gaaatggtct gtctggtttg ttccagccgg actgtctgtg tacctgctgg
     1681 cttccacact catgattcga atttgggggg caaataaccc gtttttaatc cggcttgctg
     1741 agccgttggg acgctggttt ttcctgtccg tgctgcaggc gcttctggtt ttctttttta
     1801 ttagcgagca aaagttctcc cggggttaca aaaggaccgc ggcattcatt cttgccgcga
     1861 ttgcgctggt ctggttgtta gtccttagca cgcgcatcgg gctggaaccc gataaccgct
     1921 actggaatgt ggccggcgta cccgtgctcc tcaatcagct tggcctgatc ctgttcctgg
     1981 cgctcttcgg catcttcctt tatggtttgc tgacgcgcaa gccagccaaa aatccgcgca
     2041 ggcgcgaaat tctactggat ttgctggcgt gcctgatatt gtggattggc gctgcctggt
     2101 tctggcacca ggcgcctttt tccaacagtt ttttcgcgga agggaaattt ccgccaaacc
     2161 aggactttta cccatacagc gacgcggctc tgaccgattt gggcgggcag tatatgctga
     2221 ttggcaaggg gctggagtac ccatatttca ccgaaaagcc gctctacgcg ctatttttag
     2281 gacttctgca caagtttgcc gggcaaagct acctgaccgt cagcagctgg caaatgattt
     2341 gttttgcagt gctgccagtg ctgctttacc tgctggggaa acaattgcac gaccgcctgt
     2401 ttggtctttc ccttgcgctg ttttccgtcg ccaaggaata caacgccatt ttttcgacct
     2461 tcaagatatc ggtttccaat tcacgactgt acctctcgga attccccacc ctgatattca
     2521 tggtcgcttt ggcaatcgcg ctggttgcct ggttcaaaca cagcggaaaa aagaatccat
     2581 ggccgctcct tgccggcgga gctttgggtt tggctgcgat ggtgcgcacc aatccgctgt
     2641 tccttatccc ggtcatcctg ctctttggat tttggattta ccggcgcaac ctgaagcgct
     2701 attggggttc ggcgctgctg ttcttatgtg ggattttgct ggtaatcggt ccgtggttgg
     2761 catacaaccg tattaaatac ggcacggacc cgttttcgta caaaatcagc gcggtcattc
     2821 agaccagatt ccaacaacaa gtgcagcagc cggcgcctac cgggcttgac caacacgata
     2881 tcagcctcct gggtgagcag ccggtaatcc catcggacac acaacctgac gagggtgcgc
     2941 cgatgagcgc aaggctgggt gcagccgggt ttacgctggg gcacttcctc aacaacgaaa
     3001 tcaaagcgct ttttacgctg ccgtttactg tctacccgct ggaaattacc cctgtgctgg
     3061 atttgcccta ctgggaggaa ccggtaagtt ggcgcgggga gatgcccttg tcaaccgttc
     3121 tggcgtttgc cttgaacctg gcgctcattg gaattgggat agcagcgagt tggaagcata
     3181 acagcgcggc ggggctggtc ccgttgatat tgaatattgg ctactatgcc tcaaacgcgc
     3241 tcggacgaac ttcaggcagc cgctacctgc ttccggtgga ctggacgctg tatttctatt
     3301 ttctgcttgc ccttgtgctg ctgtacgaga gcggtacgca cctgcgcagt acccttcccg
     3361 aggaacaaaa acccgcatta gaccagctca gcggacagaa acgccgcccc ttgccatgga
     3421 cggtttctgc cggtttgctc ttaatcttag gcgcctcaat cccaatcatc aacctcagct
     3481 ttccggaact gtatacccat caggtgcaaa gtgagactaa aaacgcgctc ctctccagcc
     3541 cgcttttcaa ttcgaataaa gaccttacgg cacagtcgga attgctcgta aagaaacggt
     3601 cggcgcagct attcaaggga cgtatgttgt acccacgata cagagatttc gttctgaatg
     3661 gaaaagaagg tctcatcctg acggtactgc gcccggaatt gaccgaagtg tttttctcat
     3721 tcgacactga cgaccttact tacctggagg cagggcagga aatcatcgtg ctgggctgcc
     3781 aacgggaagg ttatgtggaa gcttttacgg cttatttccc ggagcagggg ctgactatgc
     3841 gctcgaataa ccctgatttt atcagcagct gtccgtaagc ttgctagtgg actgttccgc
     3901 gggctcaagg cgaggagctg ccaggcagct gtgttaaaat aacctgcagt ttatttcatc
     3961 tcatcgaaag cagtggattt gcgatgaacg cggacaaaca aactaaaacc ccccctgagg
     4021 attacgtctt gcgccttttc agcaggaaaa gcctttttcc ctggctgctg gtgctcgcgt
     4081 gcatgcttgc cggcgctttg cttggcgctt tgctgagcct ggcgctgccg cccgtctacg
     4141 aagccagagc gctggtcagc acaaatctgg aattggttca ggacgcgaac atcaccgaaa
     4201 ttatgctgga cgcggaaatt aatcacatcg gcgaattggt tttccatcct gaggttgtga
     4261 acgcgctgat tgaaaaagaa gccgtccagg gcaacatgtt gacgcttgaa caactgaagg
     4321 atagtacctc cgtggagcgg cagttgatga atacggtcat caaagtgcgc agcccggacc
     4381 cggagctcgc ggcgcgcatc gcctccgagt gggccgaaat cctgtttacg cgcctgaacg
     4441 aggcctacac ccacgcggta gagctctcca gcgcaaaaat gcagtacaac agcatccgcg
     4501 actgcctgac cgcgcagccc aaagcgccaa aatctttttg cgctaccctg actgcggata
     4561 acacccgcgc ggatctggag cggctgaccc aaattatcat cgcggagagc ccgcaaaccc
     4621 tcggcctgac agtagccttg aacgtcagcc agtaccagcc tgccgcgctg cccggcgaac
     4681 cgctgtacta ttccaggggc gccctaatcc tgggaggagc tctggcaggt ctggcagccg
     4741 gcttcgcgct cgctgaactc actccagggc tgaaaagaaa cgatgaagcc taagctatct
     4801 gcgcggctga aaggtgtttt ttgggcgctg ctgctggcag cactgccctt taccagcttt
     4861 ccgcttattt cacgtctatc gggcggtact tcggtcgcgc cgctttcagc cgtttttttg
     4921 ttaatcctgg cgctgttctt tttcctcccc gattttttcc gcagaccagc cgtcccgcgg
     4981 caagctttgc cgctgctgct gttcttcctg agcgcgcttg catcctcggc attggcggtt
     5041 tttctcccgt ttgagtcatt tcgcggggca gcagtgtgga gaaacgcgtt ggaagggatc
     5101 ctcaccatcg gtttaggcat tgccttttat ctcgttacca tttccgtcgt aagcgataag
     5161 caggcactga agaccacgct aaaatggatt tacctcgggg gagcgctggc catccttgcc
     5221 tcgctcgcgc aagctgcatg ttggtacgcg ctgggaacat atccagcggt attgaaaaac
     5281 ttccaggcgc tgatttccag cagcggcatt ctttacaagg cgcgcgtaac cggcctggct
     5341 ttcgagcctt cttggctggc caatcagctt aatatgctct atctaccctt gtggattggc
     5401 ttgtccgtca agggttactc ggtttttcgc cgcaagcttt ttggtctgtt cagcgtggaa
     5461 aacatcctga tgggcatggg gctgcttgtc ctgtttctca gtttcagccg cattggatgg
     5521 ttgacaacgc tggcgatggc cgcatttttg gtttttcgtg ctgcaaacaa gctcatgaat
     5581 aagtacctgg ataagcgctc gttaagaaca ggacgcgaga gcagcaaact ccgccatttc
     5641 ttcgccaaac ttggtctctg gatgagcttg ctgctggttt tcgtgcttat cctgctcttg
     5701 ctgggggtgt tcct
//
LOCUS       DAOQAD010000118         5591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_18159_length_5591_cov_6.186958, whole genome shotgun sequence.
ACCESSION   DAOQAD010000118 DAOQAD010000000
VERSION     DAOQAD010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18159_length_5591_cov_6.186958"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1949)
                     /locus_tag="PKL60_07875"
     CDS             complement(<1..1949)
                     /locus_tag="PKL60_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW96089.1"
                     /translation="MKKFSKFLSYLLVFVMLGVAIAACAPEQAPTETPTEPTETATEV
                     ATPEETAVVFEEQPFGENLPTAPTIMTPLVVAYNEFSQKFSPFFADTGYDMDVASLTQ
                     ITLLTTDRQGGIIYNAIEGETVPYNGVDYLYKGPADVKVEYDEATDTTKYTARLRVGM
                     KFSDGTPVTADDLIFSYYTFLDPSYVGSTTLSSYDIVGLKDYQTQTTSEVYDKYVALF
                     DEAYAAGPDHVWAATDAWTEEQHADVWPKLIEAIKAEIQGIYDEVWASYGTDDFTAKY
                     FNGKTAEEIKTSGADVAYAMRMWGFGSLKCTDEVAEGADPVAPADCVGPVVFVGAGTG
                     TEWDLTTTFPTMDDYYTELMAAYGNDVAAAFPYESVAGTDVLGNARSAFVGFWGPKDE
                     SMGGKGVPNISGIKKIDDYTVEVTTNGFEAPAVYNLLGIQVSPMHYYGDAAKYDYENN
                     KFGFDFGDLSKQESLTATPMGAGPYKFIKYDNKVVYFEANEYFYRGCPKIKEIQFKET
                     VSAEVASSLQTGTADAGEMTGSRARFEEVASYNSNGEITGDVITTSKVDNLGYGYIGI
                     NADTVNVAGEPGSDASKNLRKGLATVLAVYRDVAINSYYGEAASVINYPISNTSWAAP
                     QPTDEDYKVAFSTDVDGNPIYTAEMT"
     gene            complement(2055..2591)
                     /locus_tag="PKL60_07880"
     CDS             complement(2055..2591)
                     /locus_tag="PKL60_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96090.1"
                     /translation="MRRFTKDFELVTTVDEKGREKRSAEYKGSYFYFGVDAADYKRFR
                     TLAWALIPVMIALQIGAGFVANRGMYQMYIAVPYVLCFFPLVYLSAGALRLPRETAHL
                     RRDQAELTVERIKRSGTTLMICNGLVILAELVYLIFFNHGAKPGRELLFLSLVILTGG
                     VNVSLRLKARAISVNAEE"
     gene            complement(2625..4481)
                     /locus_tag="PKL60_07885"
     CDS             complement(2625..4481)
                     /locus_tag="PKL60_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNW96091.1"
                     /translation="MKPLTERASAGFGAKALLALLILGLAAGCGKTPAATSTQQTPPT
                     PLPPTPTATAVPPKVLTVCLAEEPVGLYRYDGHSGSAKASIFAALYDGPFEPGADGAY
                     SESILSAYPSIENGGISLVPTAVQPGDMVVDAYGELSVFKAGIFARPAGCGNSACAVT
                     WESGEFQMDLTRVEFNLRPDALWSDGEPLTAGDAVLSYQLAKAAALPAGAWALDRTDS
                     FIAENEHTVIWQGVPGFSPRELAPFFWLPLPAHQLASADPAALAQDPQAAREPLGWGA
                     YRLVHWDSSRSLALERNPHYFGRTESQAAFDQLIFRFIPDREEALAGLANGTCDVLDK
                     SYHLEALPKERLNALAATADLHWEDWAPVEQLVFGIQPASYDDGYAYWSDDRPDFFSD
                     ARTRTALLACLDPQTLAEQVLADWLPEDSDLTRLQLPAVEADPAALLDEVGWKDLDAD
                     PATPRAAQGVPGVSEGTPLSLRLTTGQRWLHQKAAALIVQKLGACGVGVEWRALPLEE
                     LYQPGPEGVLFGRKFDLALVSWQTGSEPLCSLYRAKAVPSEANFWVGTNLAGFADPAF
                     EAACADAQQGDGLAPAYPAAALLPHLSLWVSRPGLASSENLLWSQLESFNAD"
     gene            complement(4465..5451)
                     /gene="rsgA"
                     /locus_tag="PKL60_07890"
     CDS             complement(4465..5451)
                     /gene="rsgA"
                     /locus_tag="PKL60_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNW96092.1"
                     /translation="MASDQTPSRNATNLLQGLVIRSQAGFMSVRTAEGDVLCRVRGRL
                     RRGRAEGDLVAVGDQVLIETHEDGSGSIAQVLPRKSVLSRRLSGVNYEYQQILLANPD
                     QVVLVFACAQPDPSLRMLDRFLVVSEKQKIPAVVVANKLDLVPPERARELFEVYRRIG
                     YDLIYTSTLTGEGIPELKSRLQGKLNSLAGPSGVGKTSLLNSVQPGLALQVGSVSRGL
                     QGKGMHTTQVKQLFPLDGGGYLADVPGLRTLALWDVQGEELDAYFREIAPLVPECQFN
                     DCTHSREPGCAVRSAAESGRIDPRRYDSYLRLRFGGKLVQAEDEEIDFETFD"
BASE COUNT         1114 a         1657 c         1697 g         1123 t
ORIGIN      
        1 gtcatttcag cggtgtagat gggattgccg tcaacgtcgg tcgagaacgc aaccttgtaa
       61 tcctcatcgg tcggctgagg cgcagcccag gaggtattcg agattgggta gttgatgaca
      121 gaggcagcct caccatagta gctgttaatc gcgacatcgc ggtaaacagc caggaccgtg
      181 gccagaccct tgcgcaggtt cttggaggca tcggagccgg gttcgccagc aacgttcacg
      241 gtgtcggcat tgataccaat gtagccgtaa cccaggttgt caaccttgct ggtggtgatg
      301 acatcaccag tgatttcgcc gttggagttg taagaagcaa cttcttcgaa gcgggcgcgg
      361 gaaccggtca tttcgccggc gtcagcagtg ccagtctgga gggaggaagc aacttcagcc
      421 gaaactgttt ccttgaactg gatttccttg atcttggggc agccgcggta gaagtactcg
      481 ttcgcttcaa agtagacaac tttgttgtcg tacttgatga acttgtaggg gccggcgccc
      541 atgggggtcg cggtcaggct ttcctgtttg gacaggtcgc caaaatcgaa accgaacttg
      601 ttgttctcat aatcgtactt ggcagcatcg ccgtagtagt gcataggaga aacctgaatg
      661 ccaagaaggt tgtagactgc cggggcttcg aagccattgg tggtaacttc cacggtatag
      721 tcgtcaatct tcttgatacc ggaaatgttc gggacgcctt taccgcccat gctctcatcc
      781 ttggggcccc agaaaccgac aaatgcggat cgggcattac caagaacatc ggtgcctgca
      841 acggactcgt aggggaaagc cgcggcaacg tcgttgccgt aggcagccat gagctcggtg
      901 tagtagtcgt ccatggtcgg gaaggtagtg gtcagatccc actcagtacc agtgccagcg
      961 ccaacgaaga ccacagggcc aacgcaatca gcaggggcta ccggatcggc gccttcagca
     1021 acttcgtccg tgcatttgag ggagccgaaa ccccacatgc gcatggcata cgccacgtcc
     1081 gcgccggagg tcttgatttc ttccgcggtc ttgccgttga agtatttggc ggtgaagtca
     1141 tcggtgccgt agctggccca gacttcgtcg taaatgccct gaatttcggc cttgatggct
     1201 tcgatcagtt tcggccagac atcagcatgc tgttcttcgg tccatgcgtc agtggcagcc
     1261 cagacatggt cggggccggc ggcgtaggct tcgtcaaaca acgccacata cttgtcatag
     1321 acttcgctgg tggtctgggt ctggtaatct ttcaggccca cgatgtcata ggatgacagg
     1381 gtggtggagc caacgtaaga gggatccagg aaagtgtagt acgagaagat gagatcatcg
     1441 gcggtcacgg gggtgccgtc ggagaatttc atcccaacgc gcaggcgggc ggtgtatttg
     1501 gtcgtatcgg tggcttcatc gtattccact tttacatccg cggggccttt gtacaggtag
     1561 tccacgccgt tgtaaggcac agtttcgcct tcgatagcgt tgtaaatgat accgccctgg
     1621 cggtcggtcg tcagcagagt gatctgagtc aagcttgcga cgtccatatc atagccggta
     1681 tcggcaaaga acgggctgaa tttctgcgag aactcgttgt aagcaaccac aaggggggtc
     1741 ataatcgtgg gagcggtggg caggttttcg ccgaaaggct gttcttcgaa aacaacagcg
     1801 gtttcttccg gagtagcaac ttcggtcgct gtctcggtgg gttctgtcgg ggtttctgtg
     1861 ggtgcttgtt cgggcgcgca agcagcgatc gccacgccaa gcatcacgaa gaccaacagg
     1921 tacgaaagga atttactgaa ctttttcatt tctttctcct atttctccta gtttgaatag
     1981 agatccattg gattatagtg ctgataaatt tttccgtcaa tgcatcctct gaagattcat
     2041 atcaaattac aatcctattc ctcggcgttg acagaaatcg cgcgggcttt gagccttaat
     2101 gacacattca ccccgcctgt caggatcacc agcgacagga acagcagctc tctgccaggc
     2161 ttagctccat gattaaagaa aattaaataa accagctcag ccagaatcac cagcccgtta
     2221 catatcatta acgtcgtgcc ggagcgcttg atacgctcca cggtgagctc ggcctggtcg
     2281 cgccgcagat gcgcggtctc acgcggcagc cgcagagcgc ccgcgctcag gtacaccagc
     2341 gggaaaaagc acaggacgta cgggacggcg atgtacatct gatacatgcc gcggttggca
     2401 acgaaacccg ctccaatctg caaggcaatc atcacaggga tgagcgccca ggcgagcgtc
     2461 cgaaagcgct tgtagtccgc ggcatccacc ccaaaataga aatagctgcc tttgtattca
     2521 gcgcttcgtt tctcgcggcc tttttcgtcg accgtggtca cgagttcgaa atctttggta
     2581 aatcgtctca cttgcgctcc attgcattcg tattactcgc ggaactaatc cgcattaaag
     2641 ctctccaact ggctccaaag caggttttca ctggaggcca acccggggcg gctcacccac
     2701 aggctgaggt gcggcagcag cgcggcagcc gggtaggcag gcgccagccc atcgccctgc
     2761 tgcgcgtccg cgcaggcggc ctcgaaagcc gggtcggcaa agcccgccag gttggttccc
     2821 acccaaaagt tcgcctccga ggggactgct ttggcgcggt acaggctgca aagcggctcg
     2881 gagcctgtct gccaggagac aagcgccaaa tcaaacttgc gcccgaagag cacgccctct
     2941 gggcccggtt ggtaaagctc ctccagcggc agcgcccgcc attccacccc cacgccgcag
     3001 gcgcccagtt tctgcacgat cagggcggca gctttttggt gcagccatct ctgaccggtg
     3061 gtgagcctga ggctgagcgg ggtgccctca ctcacgcccg gtacgccttg cgccgcgcgc
     3121 ggcgtagcag ggtccgcgtc caggtctttc cagccgactt cgtccagcag cgcggcgggg
     3181 tccgcttcca cagccggcag ctgcaggcgc gtcaggtcgc tgtcctccgg cagccaatcc
     3241 gccaacacct gttcggccag ggtttgcggg tccaggcagg ccagcagcgc ggtgcgcgtg
     3301 cgcgcgtcgg agaagaaatc cggccggtcg tccgaccaat aagcgtagcc gtcgtcgtaa
     3361 ctggcgggct ggatgccaaa caccagctgt tccaccggcg cccaatcctc ccagtgcagg
     3421 tcggcggtgg cggcaagcgc gttgagccgc tctttgggca gggcctccag gtgataggac
     3481 ttatcgagca cgtcgcacgt gccgttggca agccccgcaa gcgcttcttc gcggtcgggg
     3541 atgaaccgga aaatcagctg gtcaaaggca gcctggcttt ccgtcctgcc aaagtaatgc
     3601 ggattgcgct caagcgcgag gctccggctg ctgtcccaat gaaccagacg gtaggcgccc
     3661 cagcccaaag gctcgcgcgc ggcctgcgga tcctgggcca gcgccgccgg gtccgcgctg
     3721 gcaagttggt gcgcgggcag cggcagccag aagaacggcg caagctctcg cggggagaag
     3781 ccgggtacgc cctgccaaat tactgtgtgc tcgttttccg cgataaaaga atcagtgcgg
     3841 tccagagccc aggctcccgc gggcagggct gcggctttgg caagctggta agacaatacg
     3901 gcgtccccgg cagtgagcgg ctcgccgtct gaccaaagcg cgtccggccg cagattgaac
     3961 tccacgcggg tcagatccat ctggaattca ccgctctccc aggtcacggc gcaggcgctg
     4021 ttcccgcagc cggcgggccg agcgaaaata cccgccttga acacgctcag ttcgccgtac
     4081 gcgtccacca ccatgtcgcc gggctgtact gcagtcggaa caaggctgat gccgccgttt
     4141 tcaatgctgg gataggcgct caggatggat tccgaataag ccccatcggc gcccggttca
     4201 aacgggccgt cataaagcgc cgcgaaaatg cttgctttcg cgctgcccga atgtccgtcg
     4261 taacgataca ggcccacggg ttcctctgcc aggcaaaccg tcagcacctt tggcggcacg
     4321 gccgtcgcgg taggcgttgg gggcagcggc gtagggggtg tttgctgtgt ggaagttgcc
     4381 gccggtgtct ttccgcagcc agccgccagg cctaaaatca acaacgcgag cagagctttc
     4441 gccccaaagc ccgcagacgc gcgttcagtc aaaggtttca aaatcaatct cctcgtcttc
     4501 agcctgcacc agtttcccgc caaagcgcag gcgcaggtaa gagtcgtaac ggcgcgggtc
     4561 aatccggccg ctttccgccg cgctgcgcac ggcgcagccg ggttcgcggc tgtgggtgca
     4621 gtcgttgaat tgacattcag gcaccagcgg agcaatctcg cggaaataag cgtccagttc
     4681 ctcgccctgg acatcccaca gcgccagggt gcgcagcccc gggacgtctg ccaggtaacc
     4741 gcccccgtcc aacggaaata gctgcttgac ctgggtggta tgcatgcctt tcccttgcag
     4801 gccgcggctg acggagccca cctgcagcgc caggccgggc tgcacgctgt tgagcaggct
     4861 ggttttccct acgcccgaag gacccgccag ggaattgagc ttgccctgca ggcggctttt
     4921 cagttcaggg atgccttcac cggtcagagt ggatgtgtaa atcaggtcgt agccgatgcg
     4981 gcgatagacc tcaaagagct cccgggctcg ttcgggcggc accaggtcga gcttattggc
     5041 tacgaccact gccgggattt tctgtttttc tgatacgacc aaaaagcggt ccagcatgcg
     5101 caggctcggg tccggctgcg cgcaggcaaa caccagcacc acctggtcgg ggttcgccag
     5161 caggatttgc tgatactcgt aatttacgcc ggaaagccgg cgcgagagca cagatttgcg
     5221 cggaagcacc tgcgcgatgc tgccgctgcc gtcctcgtgg gtttcaatca gcacctggtc
     5281 cccgacggct accaaatccc cctccgcgcg cccgcgccgc agcctgccgc gcacgcggca
     5341 gagcacatcg ccctcagcgg tgcgcacgct catgaagcca gcctgagaac ggatcaccag
     5401 cccttgcagc aggttcgtgg cattcctgga tggggtctgg tcagacgcca tacgcttggt
     5461 tacggctccg gggtttcggt ggcagggata tacggggatt cttcccaggg catggtgccg
     5521 ccggcattgg tgtagttgga gaagtagagc tgcacgaccc ggcggaaaag cggcgccgcc
     5581 gcaagcgcgg c
//
LOCUS       DAOQAD010000119         5515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_18539_length_5515_cov_6.631136, whole genome shotgun sequence.
ACCESSION   DAOQAD010000119 DAOQAD010000000
VERSION     DAOQAD010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18539_length_5515_cov_6.631136"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..1232
                     /locus_tag="PKL60_07895"
     CDS             252..1232
                     /locus_tag="PKL60_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNW96093.1"
                     /translation="MAQKILGYIQLVWTCDHCGTQNPGAIKSCTSCGAPQPPDVQFSK
                     VDPETFDFIKDEALIRMAQSGPDKHCPYCGTRNLSEAQRCIQCGSDITVGAKVRESGS
                     ILEKPTPPQPSAPARKLSPVALILLLVGIGLLCGLMFLFISKLTRTDQVSAVVSGGTW
                     QRSLQILAYQQVSESDWESAVPAGAQIESCSMQYRYDSDTPRANATEVCGEPYTIDTG
                     TGIGQVVQDCVYRVYENYCTYTSLDWVPIDTLVVSGQDLNPYWPSTALSETQRYGTQD
                     ERYTITFLSGADTYTYTTSDADLYMLAQPGSAWTLEVNQYGSVASAEPAR"
     gene            complement(1650..4142)
                     /locus_tag="PKL60_07900"
     CDS             complement(1650..4142)
                     /locus_tag="PKL60_07900"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HNW96094.1"
                     /translation="MDINGSDFYFGRQYDLVSKLALEDKPVFYDPTDLTTHAVITGMT
                     GSGKTGMGIILLEEAALQGIPAILIDPKGDLTNHLLHFPSLLPSDFAPWIDTDAAARE
                     GKTVQQAAEDASTSWSKGLSDWGIDKARLEKLAKAVDYAIYTPGSDSAIPVSILSSLK
                     CPDISWNENKEMLRENIASTVTALLELVGFKNIDPVRSREHILLANIFENSWSAGKDL
                     DLESLILQTQNPPFEKLGVFPVSKFYPENDRFELAMLLNNFLAAPAFESWLQGQPLDI
                     GSFLYSPDGKPRHSIFYLAHLADEERMFFITLLYSAIETWMRTQSGTTNLRALVYFDE
                     IVGYLPPIASPPSKPIILRMLKQARAFGVGLVLATQNPIDLDYKALSNTGTWIIGKLQ
                     TDQDKQRLLDGLDSLTGGLDRSYFDKTISALGKRVFVLHNVHAKAPLVFTTRWAMNYL
                     PGPITRNKLDDLNRMVGAEAVLASTAASGTASAPAGITAAEVKGGAREDQPGLSVEPR
                     LSSRVAVHYLPFNRNLSEALREASTQLGGTAANPLYHYQPVLLGQATVYFANRTYNLD
                     TQTKISVQIPKLEGRGLVRWEDYLSEPIDSSALDSSPLPNASFGDLVYPLDDEQNINT
                     LSRDFVEWVYRSQTLKLFSNDTLKLVSHPGESREAFEARCAAASSGSSSEGVEKIRDK
                     YAKLKKTIEDKKLREELELEKDQTVLKQRRGEETIKGLENVAKLFTGRKSNLSSSMSK
                     RRMTATAKANVQESEEMIRKYEAELADLDAKMEKEIKDYQAKGTQSAGTIREVSVAPL
                     KKDIVVELFGLAWKPVYAFKKGSDWLLVSGHQ"
     gene            4330..4818
                     /locus_tag="PKL60_07905"
     CDS             4330..4818
                     /locus_tag="PKL60_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNW96095.1"
                     /translation="MFRPMRRVAQQLPEGEALEILKNGSSGVLAVAGDEGYPYAVPLS
                     YAYSEGKLFFHVARSGHKLDAIARNDKVSFCVVAQDKVVPENFTTRYRSVVVFGRARV
                     LTDDAEKRRALRLLVEKYSPGFLAEGEDEITSAWERFHALELRIEHMTGKAAKDLIAA
                     AM"
BASE COUNT         1105 a         1515 c         1685 g         1210 t
ORIGIN      
        1 acccgaccta cgacttctct gctcaaaagg atacatttcc tctacccaat ccccacggcg
       61 atcggaggtt tcccaccagg tttctctcct gagtccctgc gctcaaccta cgacgctcac
      121 aagatatatc cgcatgttcc ttcaatcttt ctgaatttcc aacgtttgct tccagaaatt
      181 aaacatcgct ggagcggctt gatataatag agatgagcgc gcgagcactc cgaaacagga
      241 gaagagccgg catggcacaa aaaatactcg gttacatcca attggtttgg acctgcgatc
      301 actgtggaac gcaaaatccc ggcgccataa aatcctgtac cagctgcggc gctcctcaac
      361 caccagacgt gcagttcagc aaggtggacc ccgaaacctt tgacttcatc aaggatgaag
      421 cccttatccg catggcgcag agcggaccgg acaagcactg cccctactgc ggcacccgca
      481 acctgtccga agcgcagcgc tgcatccagt gcggcagcga catcacggtc ggcgccaagg
      541 tgcgcgaaag cggcagcatc cttgaaaaac ccactccccc ccagcccagc gccccagcgc
      601 gcaaactctc ccccgttgca ctgattttgc tcctcgtcgg gataggcttg ctctgtgggc
      661 tgatgtttct gttcatcagc aagctgaccc gtacggacca ggtcagcgcg gtggtcagcg
      721 gcggaacctg gcagcgcagt ctgcaaattc tggcgtacca gcaggtgagc gaaagcgatt
      781 gggaaagcgc agttccggcc ggcgcgcaaa ttgaaagctg cagcatgcag taccgctatg
      841 attccgacac gccgcgggcg aacgccaccg aagtatgcgg tgagccctac accatcgaca
      901 ccggcaccgg tatcgggcag gtggtgcaag actgcgttta tcgggtctac gagaattact
      961 gtacctatac cagcctggac tgggtgccaa tcgatacgct ggtagtttcc ggacaggacc
     1021 tgaaccccta ctggccttct accgccctga gcgaaaccca acgctacggc acacaggacg
     1081 agcgttacac gattaccttc ctttccggcg cggataccta cacgtacacc acctcggacg
     1141 ccgacctgta catgctggcg cagccgggca gcgcctggac gctggaagtg aaccagtatg
     1201 gttcggttgc ctcggcggag cccgcgcggt aaacgaatgc tttcctgcct gtcgccggaa
     1261 attgactcgc tgtaatcatt agcctttgcg ggcgagtttt tttatcccat agtgagcgaa
     1321 aagagggcct ccaaagccgc ctcgcagcgt tattttcccc catgcttagg ccctggtcga
     1381 aaaaccaatg gaaacagggc aggcttaact tcacgccggt gcaaatgcat aggctgcccc
     1441 acagctcagg cgctgtgcaa aaaaagacga ggctgtccaa atatgaacag gtttttctgt
     1501 ttagacagtc ctaagtggga gggtttgcct ggctccgatc tcgtattgat agcgcaaatc
     1561 cgcgaacgcc cagaaaaaag ggcacgcagt gggagaacca aaggggctgt ccgacttttg
     1621 ggacagcccg tctgcggtgt tctgcaagct tactgatgtc cggagaccag cagccagtca
     1681 ctgcctttct tgaaagcgta aaccggtttc cacgccagcc cgaagagctc cacgacgata
     1741 tcctttttga gcggtgcgac gcttacctcg cgaatggtac ccgcggactg cgtgcctttg
     1801 gcctgataat ccttaatctc cttctccatc ttcgcgtcca ggtccgccag ttcagcttcg
     1861 tatttgcgga tcatctcctc ggattcttgc acgttggctt tggcggtagc ggtcatgcgc
     1921 cgtttgctca tggaggagct caaattgctc ttgcgcccgg tgaagagttt cgcgacgttt
     1981 tccaggccct tgatggtctc ttccccgcgg cgctgcttga ggacggtctg gtctttctcc
     2041 agctccaatt cttcgcgcag cttcttatcc tcgatggtct ttttcagttt ggcgtacttg
     2101 tcgcggattt tttccacgcc ttccgaactg ctgccgctgc tggcggcagc gcagcgcgct
     2161 tcgaaagctt cccggctttc cccgggatgg gagaccagct tgagggtgtc gttgctgaaa
     2221 agtttgaggg tttgcgagcg gtagacccat tcgacgaaat ccctggacag cgtgttgatg
     2281 ttttgctcgt catccagcgg atagaccagg tccccaaagc tggcgttcgg gagcgggctg
     2341 ctgtccagcg cggaactatc gattggttcg ctcaggtaat cctcccagcg caccagcccg
     2401 cggccttcca atttggggat ttgcacgctg attttggtct gcgtgtccag gttgtaggtg
     2461 cggttggcaa agtaaaccgt cgcctgcccc aaaaggaccg gctggtaatg gtaaagcggg
     2521 ttcgcggctg tgccgcccag ctgcgtgctg gcttcccgca gggcttcgga gaggttgcgg
     2581 ttgaagggca ggtagtgcac agccaccctg gaggacaggc gcggctccac gctcaggccc
     2641 ggctggtcct ccctggctcc gcctttcact tccgcggcgg taataccggc tggagcgctg
     2701 gctgtaccgc tcgcggcggt ggaggcaagc accgcttccg cgccgaccat gcggttcagg
     2761 tcgtccagct tgttgcgcgt gattgggccg ggcaggtagt tcatcgccca gcgcgtggtg
     2821 aacaccagcg gcgctttggc gtgcacgttg tgcagcacga agacgcgttt gcccagcgct
     2881 gagatggttt tgtcgaaata gctgcggtcc agcccaccgg tcaggctgtc caacccgtcc
     2941 agcaggcgct gtttatcctg gtcggtttgc agcttgccga tgatccaggt gccggtgttg
     3001 gagagcgctt tgtagtccag gtcgatgggg ttttgggtgg ctaataccag gcccacgcca
     3061 aaggcgcgcg cctgtttgag catacgcagg atgatgggct tgctgggcgg cgaggcaatc
     3121 gggggcaggt agcccacgat ttcatcaaag tacaccagcg cgcgcaggtt ggtcgtaccg
     3181 gattgcgtgc gcatccaggt ttcaattgcg gagtagagca gggtgatgaa gaacatgcgc
     3241 tcttcatccg ccaaatgtgc caggtagaag atgctgtggc ggggtttgcc gtcgggggaa
     3301 tagaggaatg agccgatatc caaaggctga ccctgcagcc agctttcgaa ggccggggcg
     3361 gccagaaagt tgttcagcag catcgccagc tcaaagcggt cgttttccgg gtagaacttg
     3421 gataccggga acacgcccag cttttcgaag ggcgggtttt gggtttggag aatcaggctt
     3481 tccaggtcca ggtccttgcc ggcgctccag gaattctcga agatgttcgc gagcaggatg
     3541 tgttcgcgcg agcgcacggg gtcgatgttc ttgaagccga ccagctccag cagcgcggtg
     3601 accgtgctgg cgatgttctc gcgcagcatt tccttgtttt cgttccagct gatatccggg
     3661 cacttgagcg aggagaggat gctgaccggg atagcggagt ccgagccggg ggtgtagatg
     3721 gcgtaatcga ccgcctttgc cagcttttca aggcgggctt tgtcgatgcc ccaatccgag
     3781 agccccttgg accaggaggt tgaggcgtct tcggcggctt gctggacggt tttgccttcc
     3841 cgcgcggcgg catccgtgtc aatccagggc gcaaaatcgg agggcagcaa gcttgggaag
     3901 tgcagcaggt ggttggtcag gtcgcctttg gggtcgatca ggatggcggg gatgccctgc
     3961 agggcggctt cctccaggag gatgatgccc ataccggtct tgccggagcc ggtcatgccg
     4021 gtgatgaccg cgtgagtggt caggtcggtt gggtcgtaaa agacgggttt gtcctccagg
     4081 gcaagtttgg aaacgaggtc gtactggcgc ccgaaataga aatctgaacc gttgatatcc
     4141 atagcgcagc tcccatgatt ggtttattgg ctaagtatac tgcaaacggg caggggagat
     4201 aggcgttttt gggaggatgg atggaaagca agctgcaggg gcaaggcatc agtggcacgc
     4261 cagaccgagg acatgcaggg taaaattagg ctttgggcgc cggaggagcc ggccgggaga
     4321 ggagcaggta tgttcagacc aatgcggaga gtagcacagc agcttccgga gggtgaagcg
     4381 cttgagattt tgaaaaacgg cagttcgggc gtgctggcgg tcgcggggga tgagggctac
     4441 ccgtacgcag tgccgctgag ctacgcgtac tctgagggca agctgttctt ccacgtggcg
     4501 cgctccgggc acaagttgga tgccatcgcg cgcaatgaca aggtctcgtt ttgcgttgtc
     4561 gcgcaggaca aggtggttcc ggagaatttt acgacgcgtt accgcagcgt ggtcgtcttc
     4621 ggccgggcaa gggtgctgac ggatgatgcg gagaagcgcc gcgcgctgag gctgctggtg
     4681 gagaagtatt cgccggggtt cctcgcggaa ggggaggacg aaatcacaag tgcctgggag
     4741 cgcttccacg cgctggagct gcggattgag cacatgaccg ggaaagcggc caaggatctg
     4801 atagcggcag ccatgtagtc gttgtggaga ggcatcctgt ggcagcgcct ttgcctcacc
     4861 aaaagcacat gccaaggata ctgcgccctt atcacgggat tattctcacg gatctcagga
     4921 gcgtcctttg ctgatgtttg gagacctggc ggtcggcctc cgcgcgcggt cgggaggccg
     4981 gcgcgatctc agggtatcag gatgacaacg gattcgtcat tgcgaaccgt tttgccgtta
     5041 aaaccataac cttttgattt ctgactctca ggcaaaacgg tgcggcatag cggagcaccc
     5101 cttgtgggat ctgccttttc ttgattagct gggagatcgc ttcgttcctc gcgatgacga
     5161 atgaaatgct ctctccggtc tcctgacccc gatagaacaa ggtgttggaa aagatattcc
     5221 ttcgctgatg acccgtacct gtaagggtat caggatgaca acggattcgt cattgcgaac
     5281 cgttttgccg ttcgctcttg agcctatgcc ttccttggct ctcaggcaaa acggtgtggc
     5341 aatctgcacg aaacttgggt caaaattaca ctctatgaag caatgggaga attatttatc
     5401 gactacggcc atgcatagtg ggtgaaatgt cgattgatta acatatcggg agatcgcttg
     5461 gtgcctcgcg atgacgaatg aaatgctctc tccggtctcc tgaccccgat agaac
//
LOCUS       DAOQAD010000120         5504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_18581_length_5504_cov_5.842356, whole genome shotgun sequence.
ACCESSION   DAOQAD010000120 DAOQAD010000000
VERSION     DAOQAD010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18581_length_5504_cov_5.842356"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PKL60_07910"
     CDS             <1..422
                     /locus_tag="PKL60_07910"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNW96096.1"
                     /translation="TEPMYGQPGHAYVYFTYGMHWLLNAVTDAPGTPAAVLIRAIFPL
                     DGTARMAELRPMLVGQPGWLNGPAKLAQALAIDGKLNEADLCSAQSGLYIEEGIRVPP
                     GALRTAARVGINAVPEPWRSLPWRWQLDPDTLREALA"
     gene            492..947
                     /locus_tag="PKL60_07915"
     CDS             492..947
                     /locus_tag="PKL60_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNW96097.1"
                     /translation="MMLGKKKTVPNFIAEPSAERVTSVIGPGITWTGDLRGKGGIRIE
                     GTVDGEIAVQGLVVIGESGKVNCRTLQAERLIVAGAVKGNISCGKLEIRATGRIWGDV
                     VTTSFSSEDGAFLRGQMRMEDKVEITGTGADPHSEALENQPVETLPKEE"
     gene            complement(949..1416)
                     /gene="dtd"
                     /locus_tag="PKL60_07920"
     CDS             complement(949..1416)
                     /gene="dtd"
                     /locus_tag="PKL60_07920"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNW96098.1"
                     /translation="MRAVVQRVSRGQVNVENKLVASIQRGLVVLLGVSPTDTGADAQA
                     LAEKVANLRIFEDAVGKMNLSCLDVNGEILAVSQFTLYADTRRGRRPSFTGAAKPELA
                     EPLCARFVECLRAMGLNVQTGVFGAHMLVDLRNDGPVTILLEQPEPERFSWNF"
     gene            complement(1589..1837)
                     /locus_tag="PKL60_07925"
     CDS             complement(1589..1837)
                     /locus_tag="PKL60_07925"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNW96099.1"
                     /translation="MGLFITILVGIIAGYIAAKLMKIDVSFWIAMLLGIAGSILGGWI
                     TSLLLGVDLTTGFNLTTILVSIGGAVLVVLIYRLLKKK"
     gene            complement(1880..2320)
                     /locus_tag="PKL60_07930"
     CDS             complement(1880..2320)
                     /locus_tag="PKL60_07930"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNW96100.1"
                     /translation="MAKFEREVEIDAQVDKVWQVLTNTAYWPQWFPGIDSIANVSAVQ
                     VGGTFEWVDDGKTGTGEFLEVDPHKKLVVLTRMGNDKDKHSFELRPTGGFLGLAADEC
                     KLEYKLDNLSGAGILGNFITGGNPKDALRVKKATTALRKLVESL"
     gene            2472..3611
                     /locus_tag="PKL60_07935"
     CDS             2472..3611
                     /locus_tag="PKL60_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNW96101.1"
                     /translation="MTGPDIEEPTYVDTPTLLEEMLADLRRTSALAVDTESNSLHAYQ
                     EQVCLIQISTREHDYLVDCLALSDLSPLGAVFADANTQKVFHAGDYDLTCLKRDYAFQ
                     FANVFDTMLAASALGEESLGLAGLLEKYLGVQIDKKYQRADWGKRPIKAEMLRYAQMD
                     SHYLLGLRDSLVPRLEALGRLSIVLEDSAALASQTPAMKNHAEDFWRVRGINGMKPPA
                     LSLLKQLNHLRESLAKNQNLPPFKIMGDAALVEIANTQPHYIEELSLLPSLSAGQVRR
                     YGKELMRVIEQWRKRPGKVSRPRSQGPDDAVLRRRELIGEWRKQSGLKQGLPSNVILP
                     RDLLEKIAQADVEDLEALRCLMLESPSRFEMYGEDIFKLLKEEHP"
     gene            3608..5005
                     /locus_tag="PKL60_07940"
     CDS             3608..5005
                     /locus_tag="PKL60_07940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNW96102.1"
                     /translation="MKLHILGAAQTVTGSMYLLEHNGRKLLLECGMFQGKRDETYQVN
                     LNFPFKPAEIDAMILSHAHIDHSGNIPNLVRQGYQGWIYATNASADLADIMLRDSGHI
                     QESDLQYVNKKRRNKGQAPMEPLYTQRDAIEAMPQFRSVWYKQAFEPIPGVTAHLFDA
                     GHILGSSAVALDYQENGQSKRFWFSGDIGRLDAPILQDPVLPEKANTLLMECTYGDKK
                     HERLEDAYDEFVDAINRTVRRGGKVIVPAFAVGRTQNLSYLLSEAIDNGDIPDVPVVI
                     DSPLAANASDLYRKHTECFDDETWKYIAEHRMQGLDFDNLIFTRSVDESKALNEYKGP
                     MVIISASGMAESGRIQHHLANNIEDGRNTILIISWQAPDTLGRRLADQERRVRIFGDE
                     YYRKAEVVTIGGLSAHAGQDLLVQYALASKDTLQQLILVHGEEKAASALMEKLAEHPE
                     LPKPAYPVKGAEFEL"
     gene            5033..5123
                     /locus_tag="PKL60_07945"
     tRNA            5033..5123
                     /locus_tag="PKL60_07945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5067..5069,aa:Ser,seq:cga)
BASE COUNT         1239 a         1581 c         1633 g         1051 t
ORIGIN      
        1 gcacagaacc gatgtacggg cagcccgggc acgcttatgt gtactttacc tacggtatgc
       61 actggctgct gaacgcggtg acagacgcgc ccggcacccc tgccgcggtg ctcatccgcg
      121 cgattttccc gctggatggg actgcgcgca tggcagaact gcgcccgatg ctggtcggcc
      181 agccgggctg gctgaacgga cccgccaagc tggcgcaggc gcttgcgatt gacgggaagc
      241 tgaacgaggc agacctatgc tctgcgcaaa gcggcttgta catcgaagag ggcatccgcg
      301 taccgcccgg agcgctgcga acggccgcgc gggtggggat taacgcggtg ccggaaccct
      361 ggcgcagcct gccctggcgc tggcagctgg acccggacac cctgcgcgag gcgttggcct
      421 gaagcagccc ggaaaaaaaa gacagccgaa gctataataa ggagcagacc ccgaaaagga
      481 agcagtacca tatgatgtta ggaaaaaaga aaactgtccc aaatttcatc gccgaaccct
      541 ccgcggaacg ggttacttcc gtcattgggc cgggcatcac ctggaccggg gacttgcgcg
      601 gcaagggcgg catccgcatt gaaggcacgg tggacgggga aatcgcggta caggggttgg
      661 tggtcatcgg cgagagcgga aaagtgaact gccgcacgct ccaggcagag aggttgattg
      721 tggccggcgc ggtcaaaggg aatatttctt gcggcaagct cgaaattcgc gccaccggcc
      781 gcatctgggg cgacgtggtc actaccagct ttagcagtga ggacggggct ttcctgcgcg
      841 ggcaaatgcg catggaagac aaggtcgaaa tcaccggcac aggcgcggac ccgcactcgg
      901 aagcgctgga aaaccagccg gtcgaaaccc tcccaaaaga agaataactc agaaattcca
      961 ggaaaagcgt tccggttcgg gctgctccag caggatggta accgggccgt catttctcag
     1021 atccaccagc atgtgcgccc cgaaaacgcc ggtttgcacg ttcagtccca tcgcgcgcaa
     1081 gcattccacg aaacgcgcgc acaagggctc agccagttcg ggtttggcgg cgccggtgaa
     1141 agacggccgg cgtccgcggc gcgtatccgc gtagagcgta aactgcgaga ccgcaaggat
     1201 ttccccattc acatccaggc aggaaaggtt cattttcccg acagcgtctt caaaaatgcg
     1261 caggttggcg accttttccg ccagcgcctg ggcgtccgcg ccggtgtctg tcggtgaaac
     1321 gcccaacagc accaccaggc cgcgctggat gctggcgacc agcttgttct ccacgtttac
     1381 ctgcccacga ctgacccttt gaacgactgc gcgcatgagc ttcctcctgt gtttttgctc
     1441 tgattatacc cggctgaaaa aggagcgctg gctgaagaac gcaaaaatcc gggagcttgg
     1501 agcggtaccg ggcgctaaca gaaaagcatc cgcgcttgcg gcgcggatgc tttttgtgcg
     1561 tattaaaaag cgaaattgat tcgcgcgttt actttttctt caacaggcgg tagatcagca
     1621 cgaccagaac tgcgccgcca atcgaaacaa gaatggtggt caggttaaag ccagttgtca
     1681 ggtccacgcc caggagcaga ctggtaatcc agccgcccag aatactgccg gcgatgccca
     1741 acagcatggc gatccagaag ctgacgtcga ttttcatcag ttttgctgcg atgtagccag
     1801 caataattcc gaccaggata gtgataaaca gacccatttc aatctccttc taatgtttgg
     1861 atgaatatcc aaaagccctt tacaggcttt cgaccagttt ccgcaaggcg gttgtggctt
     1921 tcttgacccg cagcgcgtcc ttggggttgc cgccagtgat gaaattacca agaattccgg
     1981 cgccgctaag attgtccagt ttgtattcga gcttgcactc atccgcggca agccccaaaa
     2041 agccgccggt cgggcgcagt tcaaagctgt gcttgtcttt gtcgtttccc atccgggtca
     2101 ggacgaccaa tttcttgtgg gggtcaactt ccaaaaactc gcctgtgccg gttttgccat
     2161 cgtccaccca ctcaaaggtg ccgccaacct gaaccgcgct gacgttcgcg atggagtcaa
     2221 ttccggggaa ccactgcggc caatacgcgg tattggtcag tacctgccat accttatcaa
     2281 cctgagcatc tatttcaact tcgcgttcaa attttgccat aatggttacc tccatgacaa
     2341 agttagatag cctgaatata acacttaatt tccagggaaa gattgggcgt ttaatgtttc
     2401 cacaggctgc gtaaggagcg ccagttgata cgcgggaaac gcggctccgc ggcggaggtt
     2461 ataatacccg catgaccggg cctgacattg aagaacctac ttatgtggat acccccacgc
     2521 tgttggagga aatgctggcg gacctgcgcc gaacgtccgc gctggcggta gacaccgaat
     2581 ccaacagcct gcacgcctat caggagcagg tctgtttgat tcagatttcc acgcgcgagc
     2641 atgattatct ggtcgactgc ctggcgctct ccgacctcag cccgctggga gcggtattcg
     2701 cggacgcgaa cacgcaaaaa gtgttccatg ctggcgatta cgacctgacc tgcctcaagc
     2761 gcgattatgc ctttcagttc gcgaacgtct tcgacaccat gctggcggct tcagcccttg
     2821 gggaagaaag cctcggcctg gcgggcttgc tggaaaaata cctgggcgtc cagatcgaca
     2881 agaaatatca gcgtgctgat tggggcaagc gccccatcaa ggcggaaatg ctgcgctacg
     2941 cccagatgga ttcccactac ctgctgggct tgcgcgatag tctcgttccc aggctggaag
     3001 cgctgggacg cttgtctatc gttttggaag acagcgctgc tctggccagc cagacccccg
     3061 cgatgaagaa ccacgcggaa gacttttggc gcgtgcgcgg catcaacggg atgaagccgc
     3121 cagcgctcag cctgctgaaa cagcttaatc acctgcgcga aagcctggca aaaaaccaga
     3181 acctgccgcc cttcaaaatc atgggcgatg ccgcgcttgt ggaaatcgcg aacacgcagc
     3241 cccactatat cgaggaactg agcttgctcc cgtcgctctc cgccgggcag gtgcgccgtt
     3301 acggcaagga attgatgcgc gtcattgagc agtggcgcaa gaggcccggc aaggtcagcc
     3361 ggccgcgcag ccaggggcct gatgacgcgg tgctgcgccg gcgggaactg atcggcgagt
     3421 ggcgcaaaca aagcggcctg aaacagggct tgccatccaa tgtcattctc ccgcgcgacc
     3481 tgctggagaa aattgcccag gcggacgtgg aagacctgga agcgctgcgc tgcttgatgc
     3541 ttgaaagccc cagccgcttt gagatgtatg gagaagatat atttaagctg ctcaaggagg
     3601 agcatccatg aaactgcata ttttgggcgc ggcccaaacc gtcaccggtt ccatgtacct
     3661 gttggagcat aatgggcgca agctgctgct ggagtgcggc atgttccagg gcaagcgcga
     3721 cgaaacctac caggtgaacc tgaactttcc gttcaagccg gctgaaattg acgcgatgat
     3781 actctctcat gcgcatatcg accacagcgg caacatcccc aacctggtgc gccagggtta
     3841 tcagggctgg atttatgcca cgaacgcgag cgcagacctg gcggatatca tgctgcgcga
     3901 cagcggccat attcaggaaa gcgacctgca gtatgtcaac aaaaaacgcc ggaataaagg
     3961 gcaagctccg atggagccgc tctacaccca gcgggatgcc atcgaagcga tgccgcagtt
     4021 ccgctcggtg tggtataagc aggcctttga gcccatccca ggcgtgaccg cgcacctctt
     4081 cgacgcgggg cacatcctcg gttcatctgc ggtggcgctg gattaccagg aaaacggcca
     4141 aagcaagcgt ttttggttct cgggcgatat cggcaggctg gacgcgccca ttctgcagga
     4201 cccggtgctg ccggagaaag ccaacacgct cctgatggag tgcacctacg gcgataagaa
     4261 gcacgaaagg ctggaggacg cttacgacga gtttgtggat gccatcaatc ggacagttcg
     4321 gcgcggcggc aaggtaatcg tgcccgcttt tgcggtgggg cgcacgcaga acctgagcta
     4381 tttgctcagc gaagccattg ataacggtga tattccggac gtcccggtcg tcatcgacag
     4441 ccccctggcg gcaaacgcct cggatttgta ccgcaagcac accgaatgct ttgacgacga
     4501 aacctggaag tatattgccg aacaccgcat gcagggcctg gactttgata acctgatttt
     4561 tacgcgcagc gtggacgaat ccaaggcact gaatgagtat aagggaccca tggtgattat
     4621 ttcggcttcg ggcatggcgg aaagcgggcg catccagcac cacctcgcca acaatatcga
     4681 agacggccgc aatacgatcc tgattatctc ctggcaggct ccggatacct tgggcaggcg
     4741 cctggcggac caggaacgca gggtgcgcat tttcggggac gagtattatc gcaaggcgga
     4801 ggtcgtcacc atcggcgggc tcagcgctca cgccgggcag gatttgctgg tgcaatacgc
     4861 gctggcaagc aaagacacgc tccaacagct catcctggtg cacggcgaag aaaaagccgc
     4921 aagcgccctg atggaaaagc tggctgagca cccggaactg cccaagccgg cttaccctgt
     4981 caaaggggcc gagttcgagc tctgaaaacg ggtataatca tggcacaatc gcggagagat
     5041 gccagagcgg ttgattggga cggtctcgaa aaccgttatg gtccttgtgg ccatcgtggg
     5101 ttcgaacccc actctctccg ccagttttcc tcgaaccccc ggcctcgcgc gcggggtttt
     5161 tttccaagga agcatgatgg aaattacagc gcttgaccaa agctggaagg gcaagcgcac
     5221 accctttcat tctgtcagcc caaatcctcg cgcggtcatg gagacagaac gtccgccagg
     5281 ctgggaactc tcgcggcggt ggggcagcac ccaaaagccc gcgctgcggc agggaaacgg
     5341 cgggagggcg tccgtgctgt ctcggcggca ctcttcgtta tgctgtggag agtaaaaccg
     5401 gatgtcggcg ttggaagcgc cctgcctgga cgcccttgta caggtatgcg cacccgggaa
     5461 aggaatagga cagcgagcct ttcacctcgc gggctctgta tgag
//
LOCUS       DAOQAD010000121         5447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_18849_length_5447_cov_6.193991, whole genome shotgun sequence.
ACCESSION   DAOQAD010000121 DAOQAD010000000
VERSION     DAOQAD010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18849_length_5447_cov_6.193991"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1467)
                     /locus_tag="PKL60_07950"
     CDS             complement(130..1467)
                     /locus_tag="PKL60_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNW96103.1"
                     /translation="MANHPRQRNAGPVAALSLMFALLALTSCASGLQGVDLPWQQNTP
                     TLTPNTAVPQLTETPALTDASPSAPTPQTAPDSLVVWLPAEMDPTGKSQAASLLNARL
                     AAFAESEGIRITVRLKSLSGSAGLLDTLTAASSAAPQALPDLILLPRRDLETAALKSL
                     VMPLDALTSIVDDEDWFPYAHEMSLIQGVVYGIPFVGDPLALIARAGAESTLPAEWQA
                     AAVPGSLFVFPADDPQALVPLTLYMALGGGVSGNQPRPNLDEETLVKMLTLLAEGRQS
                     GSIRADVTQWQSFQQSWESFLEGGGDRTAAPLSLLLALYAQGTETPETLPKLLDTGFT
                     LSSGWVWALSTADPDRGILAVRLAEYLVEPGFLGTWSEAFGRVPARPSALASWQNEAL
                     RPSLLQQSLSARFVPENNILTSIAPALRSAVLAVLRDNLSPEDAAKQAVESLK"
     gene            complement(1553..2587)
                     /locus_tag="PKL60_07955"
     CDS             complement(1553..2587)
                     /locus_tag="PKL60_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNW96104.1"
                     /translation="MTNTQSKNPRDEALLALIRKLVETPGPSGQEGLIRAAVLEEITG
                     HVDEIRTDALGNLIARVGSKQPGGLRVMVSAHIDEIGLMVTHVDANGFARFISIGGVS
                     ALTCIGGRVLFMNGARGVIGCERLEPNSAPTLEKLFIDTGALSQETCPVRVGDVCGFE
                     RPFLDMGAHLVAKSMDDRVSAAVAIQALRALKKSPHEVYFVFSVQEEVGLRGATTAAY
                     GVNPDVGLAVDVTRTGDTPRTPKMEVSLDKGPAIKVRDSSFIADPRVVNWMVAAAKAG
                     KIPYQLEVLEAGGTDGRAIQLAHAGVPAGCLSIPCRYIHSPSEMVSYADVENAVKLLT
                     RLLSEEINLG"
     gene            complement(2589..3686)
                     /locus_tag="PKL60_07960"
     CDS             complement(2589..3686)
                     /locus_tag="PKL60_07960"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96105.1"
                     /translation="MSPTKKNTQTNRKRIGKRQLDLLAELCNALAVSGDEQEVREIVL
                     REVKPLCQQVEVDALGNVLAARMGSGQDRLRVMIAAHMDEVGFMLTQSDGDGQYSFSI
                     VGGIDARQLAGKPVLVGREHIPGVIGAKAVHLTTPEERKTALKLEQLRIDLGPGGAGL
                     AKAGDRAGFATRLSAIGPSIRAKALDNRLGVFNLIELLRSAPENIDLLAAFTVQEEVG
                     LRGARVAAYRFNPQIGIAVDSTPANDLPSYDEDGENTRYNTRLDHGPAIYLMDGATIA
                     DRRLADWLMKTAAEEGISYQIRQPNPGGTDAGAIHKTRAGVPSISVSVPQRYCHSAAG
                     LIRISDWEDTLRLLSAALRRLDSKILTPLEA"
     gene            complement(3689..4747)
                     /locus_tag="PKL60_07965"
     CDS             complement(3689..4747)
                     /locus_tag="PKL60_07965"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNW96106.1"
                     /translation="MTIDLLPHLKQMLSAPGLSGYEDPIRDIVRAAWQPFCEEISVSR
                     VGSLHALKRGDGPEPRPRVMLSAHMDAIGLMVTGIAEGLLRFTQVGGIDPRILPGQMV
                     TVHGRQDLPAMVVMPPAHLLPPEAGSGVVALPYLLIDTGLRAADVEKLVRVGDIVSFA
                     QEPIELSGGALAGHTLDNRVSVAAVTHCLDILSRRKHSWDVWAVASSQEEIGVTGAFT
                     SPVEVQPDFAIAIDVTFAQSAGSADWRSFPLGEGITLAWGPNIHPALYDAITRTADEL
                     DIPFTREAAPGMTGTDAYAIQVVQSGIPCLLIGIPLRYMHTPVETVSLKDVRRAGRLM
                     AEFISALPLDFMQTLEWK"
     gene            complement(4767..5156)
                     /locus_tag="PKL60_07970"
     CDS             complement(4767..5156)
                     /locus_tag="PKL60_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96107.1"
                     /translation="MHPSFADPEQRLDRLSRQTRTRYMLILMILITIPCYILGIVLLR
                     VNRGPFASTPTPVNSPTITRTATITLTPYLTRTPTVTPTITQTLTPTLTHTPTPTPTY
                     TNTPELTEPPVSTNTPGPEITPTGSEP"
     gene            5278..5400
                     /locus_tag="PKL60_07975"
     CDS             5278..5400
                     /locus_tag="PKL60_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96108.1"
                     /translation="MASKDKAGKEERKKPKMDIKEKRKAKIEKKKAGTVITPNA"
BASE COUNT         1070 a         1626 c         1701 g         1050 t
ORIGIN      
        1 gggcgggaat gttaaaaccc ggcagcgcaa ggaccagggc gaccaatccg agcaccactg
       61 ccatgagaat caggattaat atcgcctggt tggtacgccc gcggggaagt ttcttggggg
      121 tattagcgct cattttaaac tctccacagc ctgtttcgcg gcatcttccg ggctgagatt
      181 atcgcgcagt acggcgagca ccgcgctgcg caaagccggg gcaatgctgg tcagaatgtt
      241 gttttcgggg acaaagcgcg cgctgaggga ttgctgcagc agagacggac gcaaagcctc
      301 gttttgccag cttgcaagcg cggaaggccg cgcaggcacg cgcccaaagg cttcactcca
      361 ggtgcccaga aagccgggtt ccaccaggta ttccgccagc cgaaccgcta aaatcccccg
      421 gtcagggtcc gcggtgctca gtgcccagac ccagccgctg ctgagcgtaa aaccggtgtc
      481 cagcagcttt ggcagcgttt cgggggtttc cgttccctgc gcgtaaagcg ccaggagcag
      541 gctcagcggc gcggcggtac ggtcgccgcc gccttccaaa aagctctccc aggactgctg
      601 gaagctctgc cattgggtca catctgcgcg gatactgccc gactggcgcc cttccgccag
      661 caaggtgagc atcttcacca gcgtttcctc gtccaggttt gggcgcggtt gattgcctga
      721 gacgccgccg cccaaagcca tatacagcgt aagcggcacc agggcttgtg ggtcgtccgc
      781 tggaaaaaca aacagacttc ccggcacagc agcagcctgc cactccgccg gcagcgtgct
      841 ttccgccccc gccctggcaa tcagcgcaag cggatccccc acaaagggga tgccgtaaac
      901 cacgccctgg atcaggctca tttcgtgagc gtaggggaac caatcttcat catccacgat
      961 gctcgtgagc gcgtccagcg gcataaccag cgactttagg gcggctgtct ccaggtcccg
     1021 ccggggcagt aggatcaggt cgggcagcgc ctgtggggca gcggaactgg ccgcggtgag
     1081 cgtatccagc agcccggcag aacccgagag gctcttcagc cggacggtga tgcgaatgcc
     1141 ctcagattcc gcgaatgccg ccaggcgcgc gttcagcaaa gaagcagcct gtgatttgcc
     1201 ggtcgggtcc atctcggcgg gcagccacac gaccaacgaa tcgggcgcgg tctgtggggt
     1261 cggggcggaa gggcttgcat cggtaagcgc cggggtttcc gtgagctgcg gtacggcggt
     1321 gttcggtgtc aaggttggcg tgttttgctg ccagggcaaa tccacaccct gaagcccaga
     1381 cgcgcaggag gtcagcgcaa gcagcgcgaa catcaggctg agcgcggcaa ccggaccagc
     1441 gtttctttgg cgaggatggt ttgccatgtt taaaccgtcg ttcgaggggg taatctcagg
     1501 tttcatcttg cttttggtta taccaccaag tcagagccgc cccttccagc ccttatccca
     1561 gattgatttc ctcgctcagc aagcgcgtga gcagtttcac cgcgttttcc acatcagcgt
     1621 acgagaccat ttcagaagga gaatggatat agcggcaggg gatcgaaagg cagcctgccg
     1681 gcacgcctgc gtgcgcgagc tgaatcgcgc gcccgtcggt gccgcccgct tccaaaactt
     1741 ccagctggta ggggattttg ccggctttgg cagccgcgac catccagttc accacgcgcg
     1801 ggtcggcgat aaatgaagag tcgcgcactt taatcgcggg acccttgtcc agcgacactt
     1861 ccatcttggg tgtgcggggg gtatctccgg tgcgggtgac gtccaccgcc agcccaacat
     1921 ccgggttgac gccgtaggcg gccgtggtcg cgccgcgtaa gccgacttct tcctgcacgc
     1981 tgaacacaaa atacacttca tgcgggctct ttttcagcgc gcgcagggct tgaatggcaa
     2041 ccgctgcgga tacgcggtca tccatggatt ttgccaccag atgcgcgccc atatccagga
     2101 aaggacgctc aaagccgcat acatcgccca cgcgaaccgg gcaggtttcc tgggagagcg
     2161 cgccggtatc gatgaagagc ttttcaagcg tcggagcgct gttcggttcc aggcgttcac
     2221 agccaatcac gccccgcgcg ccgttcatga agagcacccg cccgccgatg cacgtaagcg
     2281 ctgagacgcc gccaatggag ataaagcgcg cgaagccgtt cgcgtccaca tgcgtgacca
     2341 tcagaccaat ttcgtcgata tgcgcggaaa ccatcacccg cagaccgccg ggttgtttgc
     2401 tgccgacccg ggcaatcagg ttgccgagcg cgtccgtgcg gatttcgtcc acgtgtcccg
     2461 tgatttcctc cagcactgcc gcccggatca ggccttcctg cccggaaggt ccgggggttt
     2521 ccacgagttt ccggatgaga gccagcagag cttcatcgcg cgggtttttg gattgggtat
     2581 tggtcatctt atgcctccaa tggcgtgaga atttttgaat ccaggcggcg cagagccgcg
     2641 ctgagcaaac gcagggtgtc ttcccagtcg cttatgcgaa tcaacccggc cgcggagtgg
     2701 cagtaccttt ggggtacgga aacggagatg ctgggcacgc cggcgcgggt cttgtgaata
     2761 gcccccgcgt ccgtaccgcc cgggttgggc tgccggattt gataagagat gccttcttcc
     2821 gcggcggtct tcatcagcca atccgccagg cgcctgtccg cgatggtcgc gccgtccatc
     2881 aggtaaatcg cggggccgtg gtccaggcgg gtgttgtaac gcgtgttttc gccgtcctca
     2941 tcataagacg gcaaatcatt ggcgggtgtg gaatcgaccg cgatgccaat ctgcggattg
     3001 aagcggtaag ccgccacgcg cgcgccgcgc aaacccacct cttcctgcac agtgaaagcc
     3061 gccaacaaat cgatgttttc tggcgcgctg cgcagcagtt cgatcagatt gaacacgccc
     3121 aggcggttat ccagcgcttt ggcgcggatg ctcgggccaa ttgcgctcag gcgcgtggca
     3181 aagcccgccc ggtcgcctgc tttcgccagt ccggcgccgc caggacctaa atctatgcgc
     3241 agctgttcca gcttgagggc tgttttgcgc tcttccggcg tggtcaggtg cacagctttc
     3301 gcgcctatga ccccggggat atgctcccgc ccgaccagga cgggtttgcc tgccaactga
     3361 cgcgcatcaa tcccgcccac gatggagaag ctgtactgtc catcgccatc gctttgcgtg
     3421 agcatgaagc cgacctcgtc catgtgcgcg gcgatcatca cccgcagcct atcctgaccc
     3481 gagcccatgc gcgcagccag gacgttcccc agcgcgtcca cttccacctg ttgacacaaa
     3541 ggtttcactt cgcgcaggac gatttcgcgc acttcctgct catcgccaga aacagccagg
     3601 gcgttgcaca gctcggccag caggtcgagc tgccgttttc caatccgttt ccggttggtt
     3661 tgggtattct ttttcgtcgg gctcatgttc acttccattc cagagtctgc ataaaatcca
     3721 gcggcagcgc gctgataaat tccgccatca aacgccccgc gcggcgcaca tccttgagag
     3781 aaaccgtctc cactggcgtg tgcatatagc gcagcgggat gccaatcagc aggcagggga
     3841 tgccggactg gacgacctga atggcgtaag cgtcggtgcc ggtcattccg ggcgctgctt
     3901 cgcgcgtgaa ggggatatcc agttcatccg ccgtcctggt gattgcatcg tacagcgcgg
     3961 gatggatgtt ggggccccat gccagggtga tgccctctcc caaggggaag ctgcgccagt
     4021 cagcgctgcc cgcgctttgg gcaaaggtta cgtcaatcgc gatggcaaag tccggctgga
     4081 cctcaaccgg ggaagtaaat gcgccggtaa caccaatttc ctcctggctg gaagctaccg
     4141 cccacacgtc ccagctgtgc ttgcggcggg agaggatatc caggcagtgc gttaccgccg
     4201 ccacggaaac gcggttgtcc agggtgtgcc ctgccagcgc gcctccgctc aattcgatgg
     4261 gttcctgggc aaaggagacg atatcgccca cgcgcaccaa tttttccaca tctgcggcac
     4321 gcaggccggt atcaatcagc agatacggga gcgcgaccac cccgctgcca gcttcgggcg
     4381 gcagaaggtg ggccggcggc atcaccacca tcgccggcag gtcctgccgt ccatgcacgg
     4441 taaccatctg gccgggcagg atgcgcgggt caatgcctcc gacctgcgtg aagcgcaaaa
     4501 ggccctcagc gatgccggtc accatcagcc cgatagcgtc catgtgcgcg gagagcatca
     4561 cacgcgggcg gggttcgggg ccgtcgccgc gtttgagggc gtgcaagctg cccacgcggc
     4621 tgaccgaaat ttcttcgcag aagggctgcc aggcagcccg gacgatatcc cggatcgggt
     4681 cttcatagcc cgacaggccg ggcgcggaca acatttgttt cagatgcgga agcaagtcga
     4741 tagtcatggc actctcttca ggtagtttac ggctcagagc cggtaggagt aatttccgga
     4801 cccggtgtgt tcgtgctgac aggcggctcg gtcagctccg gggtgttggt gtaggtgggc
     4861 gtaggcgtgg gcgtgtgcgt cagcgtgggg gtgagcgttt gcgtgatggt aggggtaacc
     4921 gttggcgtgc gcgtcaggta aggcgtcagc gtgatggtgg ctgtgcgcgt gatggtcggg
     4981 gagtttactg gcgtgggggt ggaggcgaac gggccgcgat tgacgcgcag gagcacaatc
     5041 ccgaggatgt aacacggaat ggtgatcaga atcatcagga ttagcatata gcgcgtccgg
     5101 gtttgccggg aaagcctgtc caatcgttgc tccgggtcag caaaacttgg gtgcacggcg
     5161 ctgacctggg gttaatcata ccatcagcaa gcgaaatatt tttgatttgc gctataatag
     5221 acatagtcgc gacagaattg cgatggtagc agcgatagta tgagaaggag gcattcaatg
     5281 gcaagtaaag ataaagccgg caaggaagaa cgcaagaagc ccaagatgga tataaaggaa
     5341 aagcgtaaag ccaagataga gaagaaaaag gctggaacgg tcattacgcc caacgcttag
     5401 tcacacgcaa ccagaccagg catctacaaa ggtgcctggt agttttt
//
LOCUS       DAOQAD010000122         5433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_18921_length_5433_cov_5.549275, whole genome shotgun sequence.
ACCESSION   DAOQAD010000122 DAOQAD010000000
VERSION     DAOQAD010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18921_length_5433_cov_5.549275"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..649)
                     /gene="coaD"
                     /locus_tag="PKL60_07980"
     CDS             complement(125..649)
                     /gene="coaD"
                     /locus_tag="PKL60_07980"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNW96109.1"
                     /translation="MTKVLFPGTFDPIHYGHIDIARRAARLFDEVVVAVYDKPIKSLL
                     FSPEERIALVRRAFEGLPNVRVTGYQVLTVTYARQIGAQAIVRGLRVFSDFEYEFRMA
                     LANKRLEPDIDVIALITTEQNTFLSSSTVKEIASLEGDVSSMVPDFVQAALNEKFSLL
                     GRNQTLVPGTSLKD"
     gene            complement(649..3126)
                     /gene="recG"
                     /locus_tag="PKL60_07985"
     CDS             complement(649..3126)
                     /gene="recG"
                     /locus_tag="PKL60_07985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNW96110.1"
                     /translation="MASLIEKYAKIFQLEASKGYQDKAVMGGLQRLSPAWLSDAREAG
                     LPETLIAGVAGKLSSYHAQNAAARRQTLLEIGSLLQIPGMRSLPPPPVEEIVVPARVP
                     QTEQENKPAKKSENQASRKLSSAALPDEGSSGLNAPVIKIRGIGTKQAKNLEKLNVFT
                     IKDLLYLFPRRYDDYSQLKSIQQLRYGEEVTILAKVVDMRSNRTSRGKLISEVIVSDA
                     TGMLRLVWFNNPYLERSLQVGMFLTISGKVETSSGRLAMFHPDYESVDQQQLNTNRIV
                     PVYPLSRQVTQRWLRKTQFNVVQYWAARVPEFLPDLVRENAQLIPLHEAITQIHFPDT
                     HERLRLAQKRFAFDEIFLLQLGVIRQKSQWQRLSGRAYTVSDAWLAERVRGLPYALTN
                     AQGKALAEIRKDVGSPHPMNRLLQGDVGSGKTIVAVLGMSIVIEGGAQAALMAPTGIL
                     AEQHYRTLLRLLTEPDSQHSAYLAPEQVRLLTGDTAAAERRQILKGLQSGEVKLLVGT
                     HALIEDPVAFRDLQMVVVDEQHRFGVQQRAALRAKGENPHLLVMTATPIPRSLQLTVF
                     GDLDVSVMDEMPAGRLPIQTRIIYPYERHTVYELIEEQVQAGHQAFIIYPLVEQGEND
                     EVRAAVEEQQRLQAEAFPHLRVGLVHGRMKPADKDAVMLAFRNKEFDILVSTSVVEVG
                     VDIPNATMMVIEGANRFGLAQLHQFRGRVGRGDAQSFCILIPESDSAAENERLAVMTQ
                     TNDGFVLAEKDLQQRGPGDFLGTRQAGFVDLKMANLADVRLIQQARTIAEQVLEQDPE
                     LERDENQALKRAVENFWPSADGNGDVS"
     gene            complement(3395..4990)
                     /locus_tag="PKL60_07990"
     CDS             complement(3395..4990)
                     /locus_tag="PKL60_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNW96111.1"
                     /translation="MFEAGFIWLTTNQQYVNSLNVFPVPDGDTGTNMVLTMQAALAEA
                     ESKGTKNVGEMAHTFAQGALMGARGNSGVILSQIWRGFSRALDHYDHMDSALFVRALQ
                     EARSTAYKGVVRPVEGTILTVIKDMTAAVENTAGSDTKLSDLLVTIVKAGDESVKRTP
                     ELLPILKQAGVVDSGGTGLFYIFEGMLRLLQGQPLDVSNAVVKPLSELELANTLETVE
                     EGQDYEVVIDFTPRPQFSLESYYNGLSDIGTSIQVGEGDGIYRMHIHVPLDKRYEPIA
                     YTEDLGIVKKIAMENLQDQMNSKKEESAKIKFAPVAPGQVAVVAVSPGEGLTRIFASL
                     GAAAVVSGGQTMNPSVKEIVAAFENLPTDKVIILPNNKNIILAAQNAAKVSVKQIAVI
                     PTRNAPQGFAAMLRLLPEGSLEENEESMKEAISEVHTGEITTATRDVTIDDVHVEKDS
                     VIALLDGKLIISTHSIDDALSQLLTAAQVENFERITLFYGEDMTLPYVNQLADKINEK
                     YPEHEVEVHDGGQPYYQLIVAIE"
     gene            complement(5112..>5433)
                     /locus_tag="PKL60_07995"
     CDS             complement(5112..>5433)
                     /locus_tag="PKL60_07995"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNW96112.1"
                     /translation="ILPQAIKSVARHAALQSYGVISLAPANIFEAVGQLLRKNNSYGI
                     NVSADGEGLYLDINLVIEYGLRIKTVTDSVAEAVKYNVEKTMGIPVIRVNVYVRGLRI
                     SNPD"
BASE COUNT         1108 a         1571 c         1493 g         1261 t
ORIGIN      
        1 catgaattgc gtgcggatgc gcaccttttt tcaggcacgt ttttaggcgg aactttcagg
       61 cacgttttag gcggaacaaa tatgttcaag ccgcggcgct ccgaacgcgc ggttcccatc
      121 aagctcaatc cttgagcgag gtgcctggca ccagcgtctg gttgcgcccc agcagcgaaa
      181 acttctcatt aagcgccgcc tgaacaaaat ccggcaccat gctggaaacg tcgccctcca
      241 gcgaagcaat ttccttgacg gtactggaag acaaaaaagt attctgttcc gtcgtgatga
      301 gcgcgataac gtcaatatct ggctcgaggc gtttgttggc cagcgccatt ctgaactcgt
      361 actcaaaatc cgaaaagaca cgcagccccc ttacgattgc ctgagcgcca atctgcctgg
      421 cataggtcac cgtcagcacc tggtaacctg tcacacggac gtttggcagc ccttcaaagg
      481 cccgcctcac cagcgcgatg cgctcctcgg ggctgaacag aagtgatttg atgggtttgt
      541 catagacagc cacgaccact tcgtcgaaca gacgtgctgc gcgtctggcg atgtcaatat
      601 gcccgtagtg gatggggtcg aaagtcccgg ggaatagtac tttggtcatc agctcacgtc
      661 tccattgccg tccgcggacg gccagaaatt ttccacagcg cgtttgagcg cctggttttc
      721 atccctttcg agttcagggt cttgctccag cacctgctcc gcgattgtgc gggcctgctg
      781 aatcaggcgc acgtccgcca ggtttgccat cttcaaatcc acaaagccgg cctgccgggt
      841 acccaaaaag tcgcccggtc cgcgctgctg gaggtctttt tccgccagca caaagccgtc
      901 gttggtctgg gtcatcacgg caaggcgttc attttccgcg gcgctgtcac tttccgggat
      961 caggatgcag aaagattgcg cgtccccccg cccgacccgg ccgcggaatt ggtgcagctg
     1021 cgccaaacca aagcggttcg cgccctcaat caccatcatc gtggcgttgg gaatgtccac
     1081 gcctacctcc accacggaag tcgaaaccag gatgtcaaat tccttgttgc ggaaggcaag
     1141 catcaccgcg tctttgtccg ccggcttcat cctgccgtgt accaggccga ccctcaggtg
     1201 cgggaaagcc tccgcctgca aacgctgctg ttcttccacc gcagctctga cttcatcgtt
     1261 ctcaccctgt tctaccagcg ggtagatgat gaatgcctga tgcccggcct gcacctgctc
     1321 ttcaatcagc tcgtagacgg tgtgcctttc ataagggtaa ataatgcggg tttggatggg
     1381 gagcctgcca gcgggcattt cgtccatcac gctcacatcc aggtcgccaa agaccgtcag
     1441 ctgcagcgag cgcgggatgg gcgtggcggt catcaccaac aggtgcgggt tttcgccctt
     1501 cgcgcgtaaa gctgcccgct gctgcacgcc aaagcggtgc tgctcatcca caacgaccat
     1561 ctgcaggtcc cggaaagcaa ccgggtcctc gatcaaagca tgcgtgccca ccagcaactt
     1621 tacctccccg gactgtaggc ctttcaagat ttggcggcgc tccgccgcgg cggtatcgcc
     1681 agtcaacagg cggacttgtt ccggcgccag atacgcggaa tgctgtgagt ctggctcggt
     1741 gagcaggcgc aggagggtgc gatagtgctg ttccgccagg atgccggttg gcgccatcaa
     1801 cgccgcctga gcgccgcctt ctatcacgat ggacatcccc aacacggcaa cgatggtctt
     1861 cccggagccg acgtcgcctt gcagcaaacg gttcatcggg tgcggactgc cgacgtcttt
     1921 gcggatttcc gccaaagctt tcccctgtgc gttcgtgagc gcatagggca gcccgcggac
     1981 gcgttccgcc agccaggcat cgctcacggt gtatgccctg ccgctcaggc gctgccactg
     2041 gcttttttga cggatgacgc ccaattggag caggaaaatt tcatcaaacg caaagcgttt
     2101 ctgggccaaa cgcagccgtt catgcgtgtc cgggaaatgg atttgcgtga tagcttcatg
     2161 aagggggata agctgtgcat tctcgcgcac caggtcgggc agaaattcgg gcaccctggc
     2221 tgcccagtat tgcaccacgt taaactgcgt cttgcgcagc cagcgctgcg taacttgcct
     2281 gctgagggga taaaccggca caatccggtt ggtattcaac tgctgctggt ccacagattc
     2341 ataatccggg tggaacatcg ccagtcttcc actgctggtt tccaccttcc ccgagattgt
     2401 gaggaacatg cccacctgaa ggctgcgctc cagataagga ttgttaaacc agaccagccg
     2461 cagcatacct gtggcatccg aaacaatcac ttcactgata agcttgcccc tgctggtgcg
     2521 gttggatctc atatccacga ccttcgccag gatggtcact tcctcgccgt aacgcagctg
     2581 ctggatggac tttagctgtg agtaatcatc gtatcggcgc ggaaaaagat acagcaaatc
     2641 tttgatggtg aaaacgttca atttttccag gtttttcgcc tgctttgtgc caattccccg
     2701 gattttgatg accggcgcgt tcagcccgga gctgccttca tcgggcagag cagcgctgct
     2761 caacttgcgc gacgcctggt tttcggactt tttggcaggc ttgttttcct gttcggtctg
     2821 cggcacgcga gcagggacga caatttcctc cacgggcggt gggggaaggc tgcgcatgcc
     2881 ggggatttgc agcaggctgc caatctccag cagcgtttgc ctgcgggcgg ctgcgttttg
     2941 cgcgtggtag ctggagagct tcccggcgac cccggcaatc agcgtttcag gcaggcctgc
     3001 ttcacgcgcg tcggaaagcc aggcagggct caagcgctgc aggccgccca tcaccgcctt
     3061 gtcctggtaa cccttgctgg cttccagctg gaaaattttc gcgtattttt cgataagact
     3121 cgccatacct ggggataatc atacccgatt atgcttttgg attcccagtt gttttatcca
     3181 aatctggcag cacaggagaa ccaacgcaaa aaagaagctg tccaaaaaga acaggttttc
     3241 ctttttggac agccctaagg gagagaatga gccggttcag acttcgtttg gatagcgcaa
     3301 attcgcacaa gctcatacaa attggcgcgg tgcgaggaaa accaaaggag ctgtccgacg
     3361 tttgggacag ccccgtctca ggaactccac gaccctactc aatcgccacg ataagctgat
     3421 agtatggttg gccgccgtcg tgcacttcta cttcgtgttc gggatatttc tcgttgattt
     3481 tatccgccaa ttggttgacg tatgggaggg tcatatcctc gccgtagaac agcgttatgc
     3541 gctcaaaatt ctccacctgc gcggcggtga gcagttgtga aagcgcgtcg tctatcgagt
     3601 gcgtggagat gatgagcttg ccgtccagca gggcgatgac gctgtctttc tcgacatgca
     3661 catcgtcgat ggttacatcg cgggtcgcgg tagtaatctc gcccgtatgc acttcgctga
     3721 tcgcttcttt catggattct tcgttttcct ccaggcttcc ttccgggagc aagcgcagca
     3781 tggcagcaaa gccctgcggc gcgttgcgcg tggggatcac ggcgatttgt ttcacgctca
     3841 ccttggcagc gttttgcgcc gccagaatga tgtttttatt gttcggcaga atgattactt
     3901 tgtcagtcgg gaggttttcg aaagcggcga cgatttcttt cacgctcggg ttcatcgtct
     3961 gtccgccgga aacgaccgcg gcggcgccga ggctggcaaa aatacgggtc aatccctctc
     4021 ccggagaaac tgccaccacc gccacctgcc cgggcgcgac gggagcgaac ttgatcttcg
     4081 cgctttcttc tttcttggaa ttcatctggt cctgcaggtt ttccatcgcg attttcttga
     4141 cgatgcccaa atcttcggtg tacgcgattg gctcgtatcg tttatccagc gggacgtgaa
     4201 tgtgcatgcg gtagatgccg tcgccttcgc ccacctgaat gctcgtgccg atatcgctca
     4261 aaccgttata gtagctctcc agcgagaact gcgggcgcgg ggtgaaatcg ataaccacct
     4321 cgtagtcctg tccttcttcg acggtttcca acgtgttggc aagttccagt tccgaaagcg
     4381 gcttgaccac cgcattagaa acatccagcg gctgcccttg taagaggcgc agcatgccct
     4441 caaaaatgta gaacaagccg gtaccgcccg agtcaacaac cccggcctgc tttaatatcg
     4501 gcagaagttc aggcgtgcgt tttacgctct catcgccagc cttcacaatg gtgaccagca
     4561 aatcgctcaa tttggtatcg ctgcctgcgg tgttctccac tgccgcggtc atatccttaa
     4621 tgaccgtcag gatggttccc tcgaccggcc gaacaacgcc tttgtacgct gtgctgcggg
     4681 cttcctgcag cgcgcggaca aacagggcac tgtccatatg gtcataatgg tccagcgcgc
     4741 gcgaaaaacc acgccagatt tgggagagga tgacgcccga gttgccgcgc gcgcccatca
     4801 gcgcgccctg ggcaaaggta tgcgccattt cgcccacatt ctttgtccct ttggattcgg
     4861 cttccgcgag ggctgcctgc atggtcagca ccatgttggt gccggtgtcg ccgtcgggga
     4921 ccgggaagac attgagggaa ttcacgtact gctggttggt tgtcagccag atgaaaccgg
     4981 cttcgaacat taattttaat ccttcgccat ccaaagggga ctgaaccagg cggtgccgct
     5041 cagcctcaga tagaatggct atcgactttc cattcataag acctcgataa taattgattg
     5101 ataataagaa cctaatccgg attgctgatt cttaagccgc ggacgtacac gttaacccgg
     5161 ataacaggga tgcccatggt tttttcgacg ttatatttga ccgcttcagc gactgaatcg
     5221 gtaacagttt tgatgcgcaa gccgtattca attaccaggt tgatatccag gtagaggcct
     5281 tcgccgtccg ctgagacatt gatgccgtag gaattgtttt tgcgcagaag ttgaccaaca
     5341 gcttcgaaga tattggccgg ggcaagactg atgaccccgt aagactgcaa agcggcatgc
     5401 cgggcaacgg attttatcgc ctgtgggaga atg
//
LOCUS       DAOQAD010000123         5206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_20113_length_5206_cov_5.422442, whole genome shotgun sequence.
ACCESSION   DAOQAD010000123 DAOQAD010000000
VERSION     DAOQAD010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20113_length_5206_cov_5.422442"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PKL60_08000"
     CDS             <1..808
                     /locus_tag="PKL60_08000"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNW96113.1"
                     /translation="LSTTIEAYKPLQEKNFTTYQNHYGELSLRLEVNSYRKIRWLSAE
                     TMGTGVVDLPPSFLETCGCWLTFSPDYVDRLRDEKLWSADANQYGAGWAALKQRILQR
                     DGYRCRACGTSGDGSKLHVHHIQPFKTFEDPDKANAPANLITLCPACHQQAEITVRLR
                     SGLAGAAYALGNLAPLMVMCDREDLGMLSEARSVLAQGGPAILVYDNVPGGIGLSERL
                     YERQEFWISKAAEMIADCQCQDGCPACVGPIGEEGHGGKQEALALLSGLV"
     gene            810..2075
                     /locus_tag="PKL60_08005"
     CDS             810..2075
                     /locus_tag="PKL60_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNW96114.1"
                     /translation="MDFGSLSEKLKKMGVSLGMQPEPTSARKTRKLIEQVVEGREIQT
                     NFGSLFSSNHAYPEDYRLGNQALLPARPTQGIARWARTPHLAETSIENFIFLDTETTG
                     LSGGTGTMAFMVGVGRYRGGRFVMEQFFLRGPAEEAALLAALEEFCRDMAAVVTYNGK
                     SFDIPILNARYILQGFTSPFEDLPHLDLLHLTRRIWKARLEQCNLGNIEDKIFGLSRD
                     ANEVPGYLIPEYYTQYLREGDAQPLKGIFVHNEQDVVSLAALFALFTDVLHDPAAWQN
                     DSSQDLTSLGRLFEKLGDADSALSLYEKGRRVGGSPEPRVEPLLAQARLLKRQNRYQE
                     AEALWLQAAESGALEALEELAKFHEHQRGNPQIALEFTEQALSVMSRFSPEARSAQRE
                     AAFLHRRERLRSKLSRQRFESGGQKAVSD"
     gene            2637..3392
                     /locus_tag="PKL60_08010"
     CDS             2637..3392
                     /locus_tag="PKL60_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNW96115.1"
                     /translation="MSGHSHWSTIKRKKGAADAKKGAVFTRLAREIALAAREGGGDPS
                     MNVSLYLAIDRARSSNMPKDSIERAIKRGTGEDKSAAELEELTYGGTLGHGIVAMIEC
                     VTENRNRTVAELRHILTRAGGNLADTSSISWQFDRKAVFSVKSDGKNFDKVFEAALEG
                     GADDVLEDDGYIEIVGSVESFKTIGDSLKKFNIKPEEAGLRMVAQQEMELDKEKTISI
                     LRTIEALEESDDVQNVYSNLKITEEALADLEKE"
     gene            3399..3905
                     /gene="ruvC"
                     /locus_tag="PKL60_08015"
     CDS             3399..3905
                     /gene="ruvC"
                     /locus_tag="PKL60_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNW96116.1"
                     /translation="MLVLGIDPGIAITGYGLVTVDPAGSPALVSFGVIDSTAVSSTPS
                     RLLFLYEHLREILAEYQPTHAAMEKIFFQKNLKTVSAVSEARGVISLCLEQGRLPLAE
                     YTPNEVKLSVTSYGNAHKPQVQEMVRILLNMDAIPKPDDAADALAVALCHIGYLNFSS
                     SAADKGEA"
     gene            3902..4714
                     /locus_tag="PKL60_08020"
     CDS             3902..4714
                     /locus_tag="PKL60_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNW96117.1"
                     /translation="MITSLTNPGIVAARKLLLPKERKSTGNFLAEGLRVVAQAFDSGA
                     QIQQIICSPELLVSEFGQGLVEKARTRGIPILEVSQAVFASLARKDTPQGIAAVIAQR
                     WFPLDESAPVETDLWVALQAVQNPGNLGTVLRTCDAVGAKALLLLDDCTDPYDAAAVK
                     ASMGSIFTVRLMKSRLSEFQTWALSRPDLSIFGSSDKGAEDYTLARYSLPALVLMGSE
                     RQGLPAEYLALCRQTVRIPMEGACDSLNLSIAAGIILYEIYNQNRKRRIEPL"
     gene            4711..>5206
                     /gene="ruvA"
                     /locus_tag="PKL60_08025"
     CDS             4711..>5206
                     /gene="ruvA"
                     /locus_tag="PKL60_08025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNW96118.1"
                     /translation="MIASISGKVQSKLADSLILDVQGLGFEVTVTKSVLSEVEPADIV
                     FLHTYLVVREDLLALYGFRTMEEKQFFLHFLGVEGIGPRLAMAILSTLSLDIIRTAII
                     SEQPDLFSRVPGIGKKTAQKILIHMQGRISAEAGFDLRKPTSSAEDEALEALESDLHQ
                     HIHLE"
BASE COUNT         1211 a         1463 c         1471 g         1061 t
ORIGIN      
        1 tttgtccacc accatcgaag catacaagcc cttgcaggaa aaaaacttca cgacctatca
       61 aaaccactac ggggaactca gcctgcgtct ggaagtaaac agctaccgca aaatccgatg
      121 gctcagcgcg gaaaccatgg gcaccggtgt ggtcgatttg ccgcccagct ttttggagac
      181 ctgcggctgt tggctgacct tcagcccgga ttatgtagac aggctgcggg acgagaagct
      241 ctggagcgcg gacgccaacc agtacggcgc gggttgggca gcgctgaagc agcgcatcct
      301 gcagcgcgac ggctaccgct gccgggcctg cggaacgagc ggagatggga gcaagctgca
      361 cgtgcatcac atccagccgt tcaaaacctt tgaggacccc gacaaagcca acgcaccggc
      421 aaacctcatc acactgtgtc cagcctgcca ccaacaagct gaaattactg ttcgcttgcg
      481 cagcgggctg gctggcgcgg cgtacgcgct cggcaatctg gctccgctga tggtgatgtg
      541 cgaccgcgag gatttgggca tgctttccga ggcgcgttcg gtgctggcgc aaggcggtcc
      601 agccatcctg gtgtacgata atgtgccggg aggcatcggc ctgtccgaac gcctgtacga
      661 acggcaggag ttttggattt ccaaagccgc tgaaatgatt gccgactgtc agtgccagga
      721 cggatgcccg gcttgcgtgg gacctatcgg cgaggaaggg cacggcggca agcaggaagc
      781 gctggcgctg ctaagcgggt tggtgtgaga tggattttgg ttccctgagc gaaaaactaa
      841 aaaagatggg cgtcagcctg ggaatgcagc ctgagccaac ttctgcccgc aagacccgca
      901 aactcattga gcaggtagtg gaaggccggg aaatccaaac gaactttggc agcctgttct
      961 cttccaacca tgcgtaccct gaagattacc ggcttggaaa ccaggcgctg ctgcctgcca
     1021 gaccgactca aggcatcgcg cgctgggcac gcaccccgca tttggcggag accagcattg
     1081 agaatttcat ctttttagac acggaaacca ccggtctttc aggcgggacc ggcacgatgg
     1141 ctttcatggt cggcgtcggc aggtaccgcg gcggcagatt cgtaatggag cagtttttcc
     1201 tgcgcggacc agctgaagaa gccgcgcttc tggcggcatt ggaggaattc tgtcgggaca
     1261 tggcagccgt ggtaacctat aatggaaaat ccttcgatat tcccatcctg aacgcccgct
     1321 acattctgca gggtttcacc agccccttcg aagacctgcc gcacctggat ttgctgcacc
     1381 tgacccgccg aatctggaaa gcgcgtctcg agcaatgtaa tctgggcaac attgaggaca
     1441 aaatttttgg cctcagccgc gacgcgaacg aagtgcccgg gtatctcatc ccggagtact
     1501 acacgcaata tttgcgcgaa ggagacgcgc agccgctcaa aggtattttc gtgcacaacg
     1561 agcaggacgt cgtcagcctg gcagccctct tcgcgctgtt caccgacgtc ctgcacgacc
     1621 cggcggcctg gcaaaacgac agcagccagg accttaccag tcttgggcgc cttttcgaaa
     1681 agctgggaga cgcagacagc gcgctttcgc tgtacgaaaa agggcgcagg gtgggcggct
     1741 cgcccgaacc ccgcgtggag ccgctgctgg cgcaggcgcg cctgctcaaa aggcagaacc
     1801 ggtatcagga agccgaagcg ctgtggctcc aggcagctga gtcaggcgct ttggaggctt
     1861 tggaggaact cgccaaattc cacgagcacc agcgcggcaa cccccaaatt gcgctggaat
     1921 ttacggagca agccctgagc gtcatgtccc gcttcagccc ggaagcccgc agcgcacagc
     1981 gagaggcggc ctttctccac cgccgcgaac gccttcggag taagctgagc cggcagcgat
     2041 ttgaatctgg cggacagaaa gctgtatccg actaatactt ttccggcggg cacaagcttt
     2101 acggcccgca gagtttctca acccgcggct tcgactttgt ttgtccaggc gaaacagaaa
     2161 tgtcctgatt cgtggatgaa gtcgtcagga tgggttttcc ctcgaaacta tgccgcttat
     2221 ttcttcttat agatcatgtt tcacgattgc accctggacc ttccccagcg gcaaggggct
     2281 ttattttgcc tcgctcaagc tgctggaaaa gggaaatacg tttggaatag cgcacagctt
     2341 ctatcaaagt tcacccccgt cttaataata gttaattagg ataaactaat aaatttcgtt
     2401 cgctgggaag gggagtgaga gggaaaacgc ggcgataacc ttgtgtatca ggtcattctc
     2461 attccattgt aaaaaaactt tgagggatcg aatgaggatt tcttagctcc tgatctaaat
     2521 caggacattt ctattttgct actttagtct ttgactttgt ctatacgccc gaatcgccag
     2581 gtggtataat aaattgttct gaagtaaacc ctagttaaaa gcgaggagat tttattatgt
     2641 ctggacattc gcactggtcc acaatcaagc gcaaaaaagg cgcagccgac gcaaaaaagg
     2701 gcgcggtatt cacgcgtttg gcacgtgaaa tcgcactggc tgcccgtgaa gggggcggtg
     2761 acccctccat gaacgtcagc ctttatctcg caattgatcg tgccaggtcc agcaacatgc
     2821 ccaaagacag catcgaacgc gccatcaaac gcggcaccgg tgaagataaa tccgcagccg
     2881 aactggaaga attgacttat ggcggaaccc tcgggcacgg catcgtcgcc atgattgagt
     2941 gcgttaccga aaaccgcaac cgcaccgtcg cggaactgcg ccacatcctc acccgagccg
     3001 gcggaaacct ggcggatacc agctccatca gctggcagtt tgaccgcaaa gcagtttttt
     3061 cggtcaaatc tgacgggaaa aacttcgaca aagtgttcga agccgcgctg gaaggcgggg
     3121 cggacgatgt ccttgaagac gatgggtaca tcgaaatcgt tggaagcgtg gaaagtttca
     3181 aaactatcgg tgatagtctg aagaaattca atatcaaacc ggaagaagcc gggctgcgca
     3241 tggtcgccca gcaggaaatg gaactggaca aagaaaaaac catcagcatc ctgcgcacca
     3301 ttgaagcgct tgaggaatcc gacgacgttc agaatgtcta ttcgaacctc aagattaccg
     3361 aggaagcgct cgcggatctt gagaaagaat aaagcccgat gctggtgttg ggtatcgacc
     3421 cgggtattgc cattacaggc tatggtctgg taacagtgga tccggcaggc agcccagcgc
     3481 tggtaagctt tggtgtcatt gattcaacgg ctgtgagttc aactcccagc cgtttgcttt
     3541 ttctgtacga acatctgcgc gaaatcctgg ccgaatacca acctacgcat gccgccatgg
     3601 agaaaatctt cttccagaaa aacctgaaga cggtttcggc tgtcagcgag gcgcgcggcg
     3661 taatcagttt atgcctggaa caaggccggc tgccgctcgc ggaatacacc ccaaacgagg
     3721 tcaaactctc ggtcacctct tacggcaatg ctcataagcc gcaggtacag gaaatggtgc
     3781 gcatcttgct gaatatggac gcgatcccaa agccggacga cgccgctgac gccctggcgg
     3841 tggcgctgtg ccacattggc tacctcaatt tttccagcag cgcggccgac aaaggcgaag
     3901 catgattacc agcctgacta atcccggcat tgttgccgcg cgcaagctcc tgctcccaaa
     3961 agagcggaaa agcacgggaa atttcctggc ggaaggtctg cgggtagtcg ctcaggcgtt
     4021 cgactcaggc gcgcaaatcc agcaaatcat ctgctcgccg gagctgctgg tgagcgaatt
     4081 tgggcagggt ctggtggaga aagcgcgaac gcgcggcatc ccaatccttg aggtcagcca
     4141 ggctgtcttc gcatccctgg cgcgtaaaga caccccccag ggcattgccg cggtaatcgc
     4201 tcagcgctgg ttccctctgg acgaaagcgc acccgtggaa accgacctgt gggttgccct
     4261 tcaggcggtg cagaatcccg gcaacctggg gactgtgctg cgcacatgcg acgcggtcgg
     4321 tgcgaaggcg ctgctgctcc tggatgactg cacggaccct tatgatgccg ccgccgtcaa
     4381 agcctcgatg ggatcaatct ttaccgtgcg attaatgaaa tccaggcttt ctgagtttca
     4441 gacctgggct ctcagccgtc cagacctctc catcttcggg tcatcggata agggcgccga
     4501 ggattacacg ctcgcccgct actccctgcc ggcgcttgtg ttgatgggca gcgaacggca
     4561 gggcttaccc gcggaatatc tggcgctatg caggcagacc gtccgcatcc ccatggaagg
     4621 cgcctgtgat tccctcaacc tttcgattgc cgcaggtata attctctatg agatttacaa
     4681 tcaaaatcgg aaacgcagaa tagagccttt atgatcgcgt ccattagcgg aaaagtccaa
     4741 tcgaaactcg ctgacagcct gatcctcgac gtgcaggggt tgggttttga ggttacggtt
     4801 accaagtcag tactctccga agtagagccg gctgacattg tcttcctgca cacctatctg
     4861 gtggtacggg aggatctgct ggccctctac ggctttcgga caatggaaga aaagcagttt
     4921 ttcctgcact ttctcggcgt ggaaggcatc ggaccgcgcc tggcgatggc aatcctttcc
     4981 acgctttctc tcgatattat ccgcacggca attatcagcg aacagcctga ccttttcagc
     5041 cgcgtgccgg gcattggcaa gaagaccgcg cagaaaatcc tgatccacat gcagggccgc
     5101 atcagcgcgg aggccggctt tgacctgcgc aaacccacca gcagcgccga agacgaggcc
     5161 ttggaagcgc tggagtccga cctgcaccag cacatccatc tcgaaa
//
LOCUS       DAOQAD010000124         5196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_20158_length_5196_cov_6.640148, whole genome shotgun sequence.
ACCESSION   DAOQAD010000124 DAOQAD010000000
VERSION     DAOQAD010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20158_length_5196_cov_6.640148"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..780)
                     /locus_tag="PKL60_08030"
     CDS             complement(136..780)
                     /locus_tag="PKL60_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96119.1"
                     /translation="MKDFRNSWDVMVKALSLWWEDWANQVLVSLSAVLLSLTVVLAVP
                     AYIGVLEQARDLTHGLRTGIAGMWLGFRRELRRSLPWGLLNLAAAALFGFTLWFYANS
                     SFVFAPTLVVLITVIAALGFIWQYFALACYTLQEEKKIRLAWKNAGALMLTQPWLVLL
                     TGLLGAGLSFVSVRYFIPLALGSPALIALLGLTAVQKTLESGTGTSQTDGKGRI"
     gene            871..1410
                     /locus_tag="PKL60_08035"
     CDS             871..1410
                     /locus_tag="PKL60_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNW96120.1"
                     /translation="MLKIEDSLLMIIDAQGNLARVVAESQPAISRIQTLIRAASILEL
                     PLLLTAQSPHKIGHTIEEIASLLPEQPELARATFSVLADEGCRQALEASGRRQLLLCG
                     FETHICVYQSAADLLAEGYDVAVIADAVSSRHMSDKQTALHEISRLGGCILSTEMALF
                     ALLRKAADPRFKAISALIK"
     gene            1655..3034
                     /locus_tag="PKL60_08040"
     CDS             1655..3034
                     /locus_tag="PKL60_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNW96121.1"
                     /translation="MTTETRNKRPKKTGRTILTVFIILALLVGAFFIIRYRQQQNAQA
                     AISDLEVVAFERKTLQSSINGTGTVQPAQDALLVWQTSGRVGELMVSIGSQVQQGDVL
                     AALDENDLPLDVLQARMDKLNVEQALANLESAAALSREQLKADISSAQASLVSLNIEL
                     SQLQERECTSWRLSNLQTDFDDAQTAYRDNPTELNLRVLQTAQSALDFCAPETIARQI
                     STLTDKISLQEQTIASWQADLEKIKDGPDPLERKKLELQLAIAEKRLESQFIKAPFTG
                     TVTSVSAVKGALVSAGTQAVRLADLSTLQLKVPVSEVDIPNVAVGQKASMTFDAFFNQ
                     SFSGQVLEIAGAAENQAGVMNYMVTISIDHGQETIKPGMTAGVVIQTAELPDVLVLPI
                     QAVVNKDGKDIVYVMRGGQPETVQVQVGAYSDGLAEILSGDLEEGEMVVLNPPTSLLD
                     SFPRLSGSR"
     gene            3039..3743
                     /locus_tag="PKL60_08045"
     CDS             3039..3743
                     /locus_tag="PKL60_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNW96122.1"
                     /translation="MTDAPQQGPVIWVRDLKKVYKMGDVEVQALRGIDLEVQPGEFIA
                     IMGPSGSGKSTLMNMIGCLDSPSSGEYWLDGQLVSDLIDDELAAVRNKKIGFVFQNYN
                     LLPRANALQNVELPLRYSADKTNLWERSAVALEKVGLADRMTHKPKELSGGQQQRVAI
                     ARAIVNEPAIILADEPTGNLDSKSGQEIMELLLSLNALNGTTLVIVTHDPGVAAQAGR
                     IVHLKDGLVNREESAL"
     gene            3740..4972
                     /locus_tag="PKL60_08050"
     CDS             3740..4972
                     /locus_tag="PKL60_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNW96123.1"
                     /translation="MKFLQALKEAVSSISSNRTRTFLTILGIVIGVGAVISLMAIGQG
                     AQQTITGQIESIGTNVIYVMKGNMTEDVTNPRDLTLLDVKALENRNRAPNIEAVSPVL
                     NANSAVKYGGESESANIIGSNTAYQKIMNLEIAEGAFFTDTEVTSGASVVVIGPELAQ
                     RLLGRNTNVVGTSLRINGQPFRVVGVTVAKGGSQFNNPDLNVYMPITSMQLRVRRGDS
                     ANQVQFIIVQARSSESMDAAISETSAVMRASHRLNPRQPNDFTITNQDDFLSIASAIT
                     SVLTIFLSGIAAISLLVGGIGIMNIMLVSVSERTQEIGLRKALGARKADIKLQFLTES
                     AMLSLIGGLLGIGLGWLLSSVVGTIAARSGTPLVPAIGLGSVLLATLFSTAIGIFFGL
                     YPASRAASLEPVEALRHE"
BASE COUNT         1191 a         1598 c         1427 g          980 t
ORIGIN      
        1 tctgcgtgta ccagtaatag tctaatggag cccagcaaag catctttttc aagaaaagcc
       61 agattcttca ctgcgttcag gatggcaggc agtttgcccc gcgaggacga gtttcatccc
      121 cttccgaatc gaaacctaaa tcctaccctt cccgtccgtt tggctcgttc ccgttccgct
      181 ttccagcgtc ttctgcacag ccgtcaaccc caacagcgcg attaacgccg gcgagcccaa
      241 cgccaggggg atgaaatagc gcacggagac gaaactaagc cccgcgccaa gcagcccggt
      301 gagcaaaacc agccacggct gcgtcagcat cagcgccccg gcgttcttcc aggctaaccg
      361 aatcttcttc tcctcctgca gcgtgtagca cgccagcgca aagtactgcc agatgaagcc
      421 cagcgccgcg atgaccgtaa tcaaaacgac cagcgtcggc gcgaagacaa agctgctatt
      481 ggcgtaaaac cagagcgtaa aaccaaacag cgccgcggct gccagattca acaaacccca
      541 gggcaggctg cgccgcagct cccgccggaa gcccagccac atgccggcaa tgcccgtgcg
      601 caagccgtga gtcaggtcgc gcgcctgttc cagcacgcca atataggccg gtacagccag
      661 caccaccgtc aggctcagca gcaccgcgga gaggcttacc aacacctggt tggcccaatc
      721 ctcccaccac aagctcagcg ctttgaccat cacatcccaa ctattccgga aatccttcat
      781 agctcctcgc tgggctgatt ataatatacg cggacgcgct gcccaaaaaa accggccagg
      841 cagcgccaac accaaccggg agaagcaaat atgctcaaaa tcgaggacag cctgctcatg
      901 attatcgacg ctcagggcaa cctggcgcgc gtggtggccg aaagccagcc tgccatcagc
      961 cgcatccaaa cgctgattcg cgccgcgtcc atcctggagc tgccgctgct gcttaccgcg
     1021 cagtccccgc acaaaatcgg gcacaccatc gaagagattg ccagcctgct gccagaacaa
     1081 cctgagctgg cgcgcgccac tttcagcgtg ctggctgatg aaggctgccg ccaggcgctt
     1141 gaagccagcg gacgccggca gctgctgctg tgcggatttg aaacgcacat ctgcgtctac
     1201 caaagcgccg ccgacctgct ggccgagggc tacgacgttg cggtcatcgc ggatgccgta
     1261 tcttcccgcc atatgagcga caagcaaacc gctctgcacg aaatctccag gctcgggggg
     1321 tgcatcctct caaccgaaat ggcgctcttt gcactcctgc gcaaagctgc cgacccgcgc
     1381 ttcaaggcga tttcagcgct gattaaataa gctgcgggtg ccgaaacctc tcagaccata
     1441 ggccgcaccc tggttttttt cacccccggg aactggcttt ttcaggcgtg tgccatgctt
     1501 gcccacagcc cacccgtctc tacgcccaag tcatccgccg cgctcccttc cagccaactt
     1561 cgctttgaaa ttgtagaaac ttcttagata gttcttatgg taagtccaat tttgaaatgg
     1621 tatagttaga acagttttca ccctggaggt atctgtgaca actgaaacta ggaacaaacg
     1681 ccccaagaaa acgggccgga ctatactgac tgtgtttatc atcctggctc tgcttgtggg
     1741 agctttcttc attattcgct ataggcagca gcaaaacgcg caagctgcca tcagcgacct
     1801 ggaagtggtt gcttttgaac gcaagacact gcaaagcagt atcaacggca ccggcacggt
     1861 ccaacctgcc caggacgcgc tgctggtctg gcagaccagc gggcgcgttg gcgagctgat
     1921 ggtcagcatt ggcagccagg tacagcaagg cgacgtcctt gcagcgctgg atgaaaatga
     1981 ccttccactg gatgttctgc aggcacgcat ggataagttg aacgtcgaac aggcgctcgc
     2041 gaatttggaa tctgccgcgg ctctcagtcg cgaacaactc aaagcggata tcagcagcgc
     2101 gcaggccagc ctggtttcgc tcaatatcga gctctcccag ctgcaggagc gggagtgcac
     2161 ctcctggcgg ctgagcaacc tgcaaaccga ctttgacgac gcgcagacag cctaccggga
     2221 taacccgaca gaactgaact tgcgcgtgct gcagacagcc caaagcgcgc tggacttttg
     2281 cgcgcctgag acaatcgcgc gccagattag caccctgacc gacaaaatct ccctgcagga
     2341 acagacgatt gcctcctggc aggcggacct ggagaaaatc aaagacggtc cggacccgct
     2401 tgagcgcaag aaactggaat tacagctcgc cattgctgaa aaacgcctgg aatcacaatt
     2461 catcaaggcg cctttcacag ggacggttac ttccgtcagc gcggtaaaag gcgcgctggt
     2521 cagcgcgggc acccaggcag tgcgtctggc ggacctgtcc accctccaac tgaaggtgcc
     2581 ggtttccgaa gtggacatcc cgaatgtagc tgtcggtcag aaagccagca tgaccttcga
     2641 cgcgttcttc aatcaaagct tttcgggtca ggtgctcgaa atcgcaggtg cggctgagaa
     2701 ccaggctggc gtgatgaatt acatggtcac catctccatc gaccacgggc aggaaaccat
     2761 aaaacccggc atgaccgcgg gagtcgtcat ccaaaccgct gaattaccgg acgtactggt
     2821 cctcccaatt caggcagttg tcaataaaga cggcaaagac atcgtctacg tgatgcgcgg
     2881 cggtcagcct gaaaccgtgc aagtgcaggt cggcgcatat tccgacggcc tggccgaaat
     2941 cctctccgga gacctggaag aaggcgagat ggtggtgctc aatccgccca ccagtctgct
     3001 ggatagtttc ccgcgcctgt ctggaagccg gtagcgctat gactgacgcc ccacaacaag
     3061 gacccgtaat ctgggtcagg gacctgaaaa aggtttacaa aatgggcgat gtggaagtgc
     3121 aggcgctgcg cggcattgac cttgaagtcc agcccggtga gtttattgcc attatgggac
     3181 cttccggctc cgggaaatca accctgatga atatgattgg ctgtctggac tccccttcca
     3241 gcggtgaata ctggctggat gggcagcttg tctccgattt gattgacgat gagctggcag
     3301 ccgtgcgcaa caagaaaatt ggctttgtat tccagaacta caacctgctc ccgcgcgcga
     3361 atgccctgca aaacgtggaa ctgccgcttc gttacagcgc tgataagacc aacctgtggg
     3421 aacgctccgc cgtggcgctc gaaaaagtgg gcttggcaga ccgcatgacc cacaagccca
     3481 aagagctttc aggcgggcag cagcagcgcg tggccattgc ccgcgcaatc gtgaacgaac
     3541 cagccatcat cctggcggac gaacccacag gcaatctcga ttccaaatcc ggacaggaaa
     3601 taatggaact gctcctgagc ctgaacgccc tcaacggcac caccctcgtc atcgtcacgc
     3661 acgacccggg cgtggcagcc caggccgggc gcatcgtcca tcttaaggat gggctggtga
     3721 accgggagga gagcgctcta tgaaattcct ccaggcacta aaagaagcgg tttccagcat
     3781 cagctccaac cgcacgcgca cgttcctgac cattctcggc atcgtcattg gcgtcggagc
     3841 ggtcatttcc ctgatggcaa tcggacaggg cgcccagcaa accatcaccg gtcagattga
     3901 gtccatcggt accaatgtga tttacgtgat gaagggcaac atgaccgagg acgtcaccaa
     3961 tccgcgtgat ttgaccctgc tggacgtcaa ggcgcttgag aaccgcaacc gcgcgccgaa
     4021 catcgaagca gtcagccctg tgctgaacgc gaacagcgcg gtcaagtacg gcggggagag
     4081 cgaaagcgcc aacatcatag gctccaacac cgcctaccag aagatcatga acctggagat
     4141 tgctgaaggc gcgttcttca cggatacaga agtgacatcc ggcgcttcgg tggtagtcat
     4201 cgggccggaa ctcgcccagc ggctgctcgg gcgcaatacc aacgtggtcg gtaccagtct
     4261 gcgcatcaat gggcagcctt tccgcgtggt gggtgtaacg gtcgccaaag gcggcagcca
     4321 gttcaacaac cctgatttga acgtctatat gccgataacc tccatgcaac tgcgcgtgcg
     4381 ccgcggcgat tccgccaacc aggtgcagtt tattatcgtg caggcgcgca gcagcgagag
     4441 catggacgcg gccatctcag aaacaagcgc ggtcatgcgc gcatcccacc gcctgaaccc
     4501 gcgccagccg aacgatttca ccatcaccaa tcaggatgat ttcctttcga ttgccagcgc
     4561 gattaccagc gtgctcacaa tcttcctgag cggcatcgcg gccatttcgc tgctggtcgg
     4621 cgggattggg attatgaaca tcatgctcgt gtctgtctcc gagcgcaccc aggagatagg
     4681 cctgcgcaaa gccctggggg cacgcaaggc ggatatcaag ctgcagttcc tgaccgaatc
     4741 ggccatgctg agcctgatcg gtgggctgct gggaatcggc ctggggtggc tgctctcctc
     4801 ggtagtcgga actatcgccg cgcgctcagg cacgccgctc gtgccggcca tcggattggg
     4861 ttccgtcctg ctggccacac tcttctccac cgcgatcggg attttctttg gcttataccc
     4921 ggccagcaga gctgccagcc ttgagccggt ggaagcgctg cgccacgagt aaaaacgcag
     4981 aggcgggggg agtatcgcga gaaattctcc atgatcccga gcttgcgaaa cggctccgaa
     5041 tttcattgag agagagattt ctgcagtcgc cgccgctcca tcacaatgac gaatctttgg
     5101 cagctttatc attggtcatc gcgagtttct tcacgcaagt tacaccgccg gacaaaggcg
     5161 gaagatgctg ccagctgggt tcaatcgggc tgcccc
//
LOCUS       DAOQAD010000125         5107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_20643_length_5107_cov_5.986342, whole genome shotgun sequence.
ACCESSION   DAOQAD010000125 DAOQAD010000000
VERSION     DAOQAD010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20643_length_5107_cov_5.986342"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKL60_08055"
     CDS             <1..195
                     /locus_tag="PKL60_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96124.1"
                     /translation="PTTTLCRVALPLHPTYDYALLGWAASPPDLRPRSVGLRCRSTRP
                     TTRVLSGCAAAPTDLRPRTG"
     gene            complement(306..635)
                     /locus_tag="PKL60_08060"
     CDS             complement(306..635)
                     /locus_tag="PKL60_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNW96125.1"
                     /translation="MIEKVYTMSTRTEHVIEPVLTDGYINYMHMVLLKDEALPTHYTN
                     ANIYMTVVTGTMHLSLSDGEFRVYPSGSVVNVPFKTKMIAQNRGEETLELIVVKSPAP
                     GSEIYKS"
     gene            839..1615
                     /locus_tag="PKL60_08065"
     CDS             839..1615
                     /locus_tag="PKL60_08065"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNW96126.1"
                     /translation="MPKPNRKDACTMTFEINSAGLYLAITILWAAMRLIRGLTTKQFD
                     LKRELAVTLLFVFLCLLSYQLFEPFRFNLERNLRANFIPIVNSLRMIQRADTARYPPL
                     IRIVLFLLVGNLVAFVPIGILAAVLFKDLRSGWKMLLLGAAFSLVVETLQVLLAVRVF
                     DVDDILLNALGTWLGYGVFRLLYLLTPFQKLFDAIASKQRKGALRFFVFASLSLGAVF
                     LGFVYWGWHAAENAGEYVLGEPEGRTLLQWFRWLFSRGYD"
     gene            complement(1762..3342)
                     /locus_tag="PKL60_08070"
     CDS             complement(1762..3342)
                     /locus_tag="PKL60_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNW96127.1"
                     /translation="MTFDVIVVGAGASGLTAAAYLAKHGRRVLLCEKEAACGGLVNSF
                     TRDGFTFDGGARALEDAGVLFTMLRQLGLQIEFVKNHISLGIEDQMLDMESETSIESY
                     AGMLKKLYPESAADIDIIARDLRLITRAMDIQYGINNPLFLDPKTDGKYFATRVFPWM
                     FKYALNVGKVQSKNQPVVPYLRQFTSNQALLDIISQHFFADTPAYFALSYFKIFQEYY
                     YPKGGTGVFIEKMAEYIQKHGGTIRTNTEIASIDPMGKTVTTSSGEQIAYRQLLWTAD
                     QKLLYNISRLDEQAHPELAAALNERKAYLADKTGSDSIFSLFVTTNQEPGYFKGIASG
                     HVFYTPSRAGQSQAGPLPLEGTPAEIQAWLEKYLALTTYEIAIPALRDPDLAPPGKTG
                     LIISVLCDYALTKRIADQGWYEEFKQLAARVMVENLDRTLYPGLAGALSQAFSATPLT
                     IQKVTGTTDGAITGWSFRNQPIPAENRLTHIANASSTILPDVSQAGQWTYSPAGLPVS
                     WITGKVAADKIQKRLGKR"
     gene            complement(3332..4267)
                     /locus_tag="PKL60_08075"
     CDS             complement(3332..4267)
                     /locus_tag="PKL60_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNW96128.1"
                     /translation="MKLLPDELQYIQNGLKKQNRSDARLDGKTCVVTGATSGVGYQSA
                     RRLAQAGARVVMLCRNAEKASLVQDELSREYQAESDVFIADMRELAQVSRAAKQIAGK
                     YPKINVLVNNAGVFNRRRRLTPDGHEEIFGVIHLAAFVLTAGLIPNLKAAAPARIIDV
                     NSEEHRFGGLKMNDLNWSRRLYIPLQAYGAAKIAQLLTGLELAGRLRGSGVSVYIMHP
                     GAVRTNIGMNNDFLYRFYNHYILGLFLIDPAVSGEAVHYLAAAPELQGVTGNFYNQTI
                     EEKPASYVLSERLRREIWDYSETVARSYLGDAYDI"
     gene            complement(4275..4742)
                     /locus_tag="PKL60_08080"
     CDS             complement(4275..4742)
                     /locus_tag="PKL60_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNW96129.1"
                     /translation="MTSEDLTTPTPLPPLTEELVLTLWSRTYNTSGKPDWSHIFPYYT
                     EDIVFQDPIQRVVGKPAFVAMCNRLTKRCQELKMDITSIASSENVIFMQWTMTMVFRK
                     TPSTPIYGCTRLSLNAAGLIAQQRDYYDLWGDIFANVPGMKRAYPSFMRKHFG"
     gene            complement(4911..>5107)
                     /locus_tag="PKL60_08085"
     CDS             complement(4911..>5107)
                     /locus_tag="PKL60_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96130.1"
                     /translation="RSGGAAAQPDKLWPAGLWSRRWVSLLRRCTQPTNWLSAAKNLNS
                     NKEQILPSVQDDNHPVIPLP"
BASE COUNT          985 a         1387 c         1525 g         1210 t
ORIGIN      
        1 cctacgacca cgctctgtcg ggttgcgctg ccgctccacc cgacctacga ctacgctctg
       61 ttgggttggg ctgcttctcc acccgaccta cgaccacgtt ctgtcgggtt gcgctgccgc
      121 tccacccgac ctacgaccag ggtcttgtcg ggttgcgctg ccgctccaac cgacctacga
      181 ccacggactg gatgaaaaac tgccagtttg taggttgggt gcagcgacgc aggagcgaaa
      241 cccaacgacg gctctacaaa accgccggcc aaagcttgtc gggttgcgct gccgctccac
      301 ccgacctacg acttgtaaat ttcgctcccg ggcgcggggg atttgaccac gatcagttcc
      361 agcgtttcct cgccgcggtt ctgggcgatc atcttggtct tgaacgggac gttcaccacg
      421 ctgccgctgg gataaacccg gaattcgccg tcggaaaggg aaaggtgcat tgtgccggtc
      481 acaacggtca tgtagatatt cgcgttggtg tagtgggtcg gcagcgcctc gtctttcagc
      541 aggaccatgt gcatgtaatt aatgtatccg tccgtgagca cgggttcgat gacatgctcg
      601 gtacgggtgc tcatcgtgta aactttttcg atcattctcg gctccttgtt ttgggttgtg
      661 atgagcattc tacaataaag cgctcgggat tgggattttg gatctttttt gcacagcctt
      721 tcaggcgcgg ggtttgtccg gacacgcttc ctgcgaaggg cgaagcaacc catgcgaatc
      781 tactttggta acctaaagca tcatcctcag aaaacagccc caaccgcgcg atgatataat
      841 gcccaaacca aaccggaaag atgcgtgcac gatgaccttt gaaatcaaca gcgctggctt
      901 gtacctggcc attaccatcc tatgggctgc catgcgcctc atccgcggat tgacaaccaa
      961 acagtttgac ctgaagcgcg agctggcggt cacgctcctg tttgtgttcc tgtgcctgct
     1021 ctcctaccag ctgtttgagc cctttcgctt caacctggag cggaatctca gagccaactt
     1081 tatacccatt gtcaacagcc tgcgcatgat tcaacgcgcg gacactgccc gctacccgcc
     1141 gctcatccgc atcgtcctgt ttctactggt tggcaacctg gtggcgttcg taccaatcgg
     1201 aatcctcgcg gcagtcctct tcaaggattt gcgctccggt tggaaaatgc tgctgcttgg
     1261 cgccgctttc tcgctggtgg tcgagaccct gcaggtgctg ctggcggtgc gggtgtttga
     1321 cgtggacgac atcctgctga acgcgctggg gacctggctc ggatacgggg ttttccggct
     1381 gctctacctg ctgacgcctt tccagaagct gttcgacgcg attgcttcca agcaaaggaa
     1441 gggggcgctg cgctttttcg tattcgccag cctgagcctc ggtgcggttt ttctcggatt
     1501 tgtgtattgg ggctggcatg ccgccgaaaa cgcaggcgaa tacgtgctgg gagagccgga
     1561 gggccgcacc cttctgcaat ggttccgctg gctgttctcg cgcgggtacg actaagctca
     1621 gccttatttt atccccttac cagatgattc agacctgccg tccggaagtt aacacggcta
     1681 atgtttcact ctactccccg gataagcttc tacccggaag tccgccagct aatcagcaaa
     1741 caaatacccc gcaggcgcct tttacctctt gcccaggcgt ttttgtattt tgtccgcggc
     1801 gactttgcct gtaatccacg agacgggcag cccggccggg ctgtaggtcc actggccggc
     1861 ctgggaaaca tccggcagga tggtgctgga ggcgttggcg atgtgggtca gccggttctc
     1921 cgccggaatg ggctggttgc ggaaggacca gccggtaatc gcgccgtcag ttgtgccggt
     1981 gaccttttgg atggtgaggg gcgtggcgct aaaggcttga gaaagcgcgc cggccaggcc
     2041 gggatagagc gtccggtcca ggttttccac cattacgcgc gccgcaagct gcttgaactc
     2101 ctcgtaccag ccctggtcgg caattctttt ggtcagggcg tagtcacata agacgctgat
     2161 aatcagcccg gtttttcctg gaggggccag gtccgggtcg cgcagggcgg ggatggcaat
     2221 ctcgtaggtg gtcaaggcca ggtacttttc gagccaggct tggatttcag ccggagtgcc
     2281 ttccagcggc agcggtccgg cctgggactg gcctgcgcgg ctgggggtgt aaaaaacatg
     2341 cccgctggcg atgcccttga aatagccggg ctcctggttg gttgtgacga acagcgagaa
     2401 aatagagtcg ctgccggttt tatccgccag gtaagctttg cgctcgttca gggccgctgc
     2461 taattcgggg tgcgcttgct cgtccagcct ggagatgttg tacaggagtt tctggtcggc
     2521 tgtccacagt aattggcggt aagcaatctg ttctccgctg gaggtcgtca cagtcttccc
     2581 cattgggtcg atacttgcga tttcggtgtt cgtccgaatt gttccgccgt gcttttggat
     2641 atattcggcc atcttttcga tgaaaacgcc cgttccacct ttcgggtagt aatactcctg
     2701 gaaaattttg aaatagctga gcgcgaagta agccggagta tctgcaaaaa agtgttggga
     2761 gatgatatcc agcagcgcct ggttggaagt gaactggcgc aggtaaggaa ccaccggctg
     2821 atttttggac tggactttac caacgttgag ggcgtatttg aacatccagg gaaaaacgcg
     2881 cgtggcaaag tacttgccgt ctgttttggg atccaggaaa agcgggttgt tgataccgta
     2941 ctggatgtcc atcgcgcggg tgatcagccg caggtccctg gcaataatgt cgatatccgc
     3001 cgcggattcc gggtagagct ttttcagcat tccggcgtaa ctctcgatgc tcgtttcgga
     3061 ttccatgtcg agcatctggt cttcaattcc cagcgagatg tggtttttga cgaattcaat
     3121 ttgcagaccg agctggcgca gcatggtaaa cagcactccg gcgtcttcca gggcgcgcgc
     3181 tccgccgtcg aaggtgaaac catcgcgcgt aaaggaattc acaagcccgc cgcaggcggc
     3241 ctctttttcg catagcaaaa ccctgcggcc gtgtttggcc agatacgcgg ccgcggtaag
     3301 gccggaagcc cccgcgccaa ccacgataac gtcaaatgtc ataagcatct cccagataac
     3361 tccgcgcgac tgtctcgctg taatcccaaa tttcccggcg caagcgctcg gagagcacgt
     3421 agctggccgg cttttcttca attgtctgat tgtaaaaatt acccgtcacg ccctgcaatt
     3481 caggcgcggc ggcaagataa tgcactgctt cccccgagac cgcagggtct atcagaaata
     3541 aaccgagaat gtagtggtta taaaaacgat agagaaaatc gttattcata ccgatgttgg
     3601 tgcgcacagc gcccggatgc atgatgtaca cgctgacgcc gctgccgcgc aagcgcccgg
     3661 ccagttccag cccggtcagc agctgagcaa tcttggcggc tccataggct tgcaggggga
     3721 tgtagaggcg tctggaccag ttcaggtcgt tcattttcag cccgccaaag cggtgctctt
     3781 cggagttgac gtcaatgatg cgcgcagggg cagcggcttt caggttgggg atgagcccag
     3841 cggtaagcac aaaggccgcc agatggatga ctccaaaaat ttcttcgtgc ccgtcggggg
     3901 tcaggcggcg gcgcctgttg aacacgccgg cgttgtttac cagcacgttt atttttggat
     3961 atttcccggc gatttgcttc gctgcccgac tgacctgggc tagctcgcgc atatcggcga
     4021 tgaagacgtc ggattcggcc tggtattccc ggctcagctc atcttgcaca aggctggctt
     4081 tttccgcgtt gcggcagagc atgacgacgc gcgcgccggc ctgcgccagc ctgcgggcgc
     4141 tttggtagcc cacgccagag gtggcgccgg taacgacgca ggtttttccg tccaggcggg
     4201 catccgaccg gttttgcttt ttcaggccgt tctggatgta ctgcagttcg tcggggagca
     4261 atttcattct tgatttatcc aaaatgtttg cgcataaagc tcggatacgc gcgtttcatc
     4321 ccggggacgt tcgcgaagat atcgccccac aagtcgtagt aatcccgctg ctgcgcgatg
     4381 aggccggcag cgttcaggct gagcctggtg catccgtaaa tcggggtgct gggggtcttg
     4441 cggaacacca tggtcatcgt ccactgcatg aagatgacgt tttcgctgct ggcaatggaa
     4501 gtgatgtcca tcttgagttc ctggcagcgc ttggtgaggc gattgcacat tgccacgaaa
     4561 gcgggcttac ccactacgcg ctggataggg tcttggaaga caatgtcttc cgtgtagtaa
     4621 ggaaaaatgt gggaccaatc gggtttgccg ctggtgttgt aggtgcgcga ccagagcgtg
     4681 agcacaagct cctctgtgag gggagggagc ggggtggggg tggtgaggtc ttccgaagtc
     4741 atcacggctc gattctaaaa taagttcctc tttttgcatt ggtaaatggt acaagatgcg
     4801 taattatagc agtatcgggc gggaattcgg gttggaaaat cgggttggac tggggaagga
     4861 tgggattgtc gggggtgaaa aggagtttct ttgccggcgg catgtgccat tcaggggagc
     4921 ggaattactg ggtggttgtc atcctgaaca gagggaagga tctgttcttt gttgctgttg
     4981 agattcttcg ctgcgctcag ccagtttgta ggttgggtgc agcgacgcag gagcgaaacc
     5041 caacgacggc tccacaaacc cgccggccaa agcttgtcgg gttgcgctgc cgctccaccc
     5101 gacctac
//
LOCUS       DAOQAD010000126         5081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_20810_length_5081_cov_6.382610, whole genome shotgun sequence.
ACCESSION   DAOQAD010000126 DAOQAD010000000
VERSION     DAOQAD010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20810_length_5081_cov_6.382610"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..960)
                     /locus_tag="PKL60_08090"
     CDS             complement(268..960)
                     /locus_tag="PKL60_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNW96131.1"
                     /translation="MQPGRYLRNMLAISPGEQERLAGCRALVAGCGGLGGFVLEYLGR
                     LGVGNLRAVDPDSFSEGNLNRQLLSSSMNLGRPKALAAVQRMQAVNPLARVEGVQALL
                     TEENAAQLLEGCQVALDCLDSIPARRTLQKACARAGVPLVHGAIGGWLGQVCVVLPGE
                     NLLDQVYPDADAEHGEEYEQGALVFTAGMVAAVQAAEAVKLLLGKPGLRGELLVLDLL
                     NGSFERLRLAKG"
     gene            complement(964..1161)
                     /gene="thiS"
                     /locus_tag="PKL60_08095"
     CDS             complement(964..1161)
                     /gene="thiS"
                     /locus_tag="PKL60_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNW96132.1"
                     /translation="MISVNTREQPWRDGLNVQALLDEMGYVFRHIIVRVNGEYVPEEA
                     YARRLIADGDDVQVLHLIAGG"
     gene            complement(1158..3290)
                     /locus_tag="PKL60_08100"
     CDS             complement(1158..3290)
                     /locus_tag="PKL60_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNW96133.1"
                     /translation="MGKILKQIHYTPAKIERGYSNKSLYINVGDLRIEEKEVTGFMKE
                     KFVGGKGFDLWYLWNAVTPDTRWDSPENEIVMSVGPLGGITQYSGTGKTLVCAISPLT
                     GIPIDSNAGGYFGPLLKFAGWDALELQGKAEKDLIIFIDGNEGVISIEEGEEYPEDSH
                     LLAEELTTRFAGSEEDKVHVSVVSSGSAAKHVLMGMLNFSFYDPRRKTVRFKQAARGG
                     LGTILRDKHIKALVVKFKGIKPNLNNPADIAALNRIGVKYHKEMHDLDEKQNGMRKIG
                     TANTILVMNKYDLLPTRNFQTGGDPEAVKISPEVFITQYLTQGLHDGCWYGCTMSCAK
                     AADHFKLLTGPYAGQCVTVDGPEYECAAGLGANLGIFDPQAILEQNFYCDTYGIDLIS
                     YATTVAFIMECYEREQIGPEHTGGLDLRFGNAAASLELLHQMSRGEGFGLLAGLGIRR
                     LKQVFVERFGADPKLLEDIGMENKGLEYSEYLHKESLAQQGGFALTNKGPQHDEAWLI
                     FMDMVNNQIPTFEDKAYALFYYPMFRTWFGLCGLCKLPWNDIVPADNYKTDTPAKVPE
                     HVQNYVDIFNAVTGKKIDNDELIRQSERVYNFQRVFNLRMGSGLRKHDKAPYRSLGPV
                     TAEEYLSRQERYDKQLRELQGLDPETLSLEEKIAATRTWREKQFELLTDAVYKRRGWT
                     NDGVPTPEHLKEIGMDLPMLLEVVEKHL"
     gene            complement(3294..3692)
                     /locus_tag="PKL60_08105"
     CDS             complement(3294..3692)
                     /locus_tag="PKL60_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNW96134.1"
                     /translation="MKVLTHKQEICTGCRACELACSQAYFRTDDRLKSAIRINNNFNT
                     FHAIMCTQCGECINMCTADAIHRDKNGVVRIDRKKCVGCLGCVGFCSELSMFYSDDLL
                     VPFKCIACNICVKACPEGALEILVSQSKGD"
     gene            complement(3805..4755)
                     /locus_tag="PKL60_08110"
     CDS             complement(3805..4755)
                     /locus_tag="PKL60_08110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNW96135.1"
                     /translation="MTQPRCSIIIRSYNEEKHIARLLEGIARQSVQDAEIILVDSGSS
                     DRTVEIAQGFSAKLVHIAPEEFTFGRSLNRGIQAANGEFLVFASAHVYPVYPDWLEKL
                     LAPFADPAIALVYGKQRGGETTKYSEEQIFASWFPDTTQPYQDSAFCNNANAAVRRAL
                     ALEHPYNETLPGLEDLDWANRILQAGYKIHYAPEAVIVHVHNETPRGVYNRYRREAIA
                     FRYIYTHERFGFVDFVRLSSRNILRDMQNASRQKVLGRHFFSILWFRVCQFWGTYQGY
                     RHHGPLTWQLRQRFYYPNGKLEEKSGQGREVEPIPYETLK"
BASE COUNT          914 a         1470 c         1605 g         1092 t
ORIGIN      
        1 tcatagatgg actcatcggc tggcggagtg tagagcgggg ggtccgaagc ttgcgcggag
       61 tgcttttcgt aggtcgggtt gcgcattaca acctgactaa ttggcggttt ggagaacgaa
      121 agatggagac ctgacggtca aactccgcgc tcggtgcggt ttcactccga ttcgcttcgg
      181 ggataataaa caccagagcg atcaagctgc cctacgactt gatacaaata gatacaggag
      241 aagaaaacag agtttgcctg ggaagctcta ccccttcgcc agccgcagcc gctcgaaaga
      301 cccgttcagc aaatccaaca ccagcagttc cccacgcagg ccgggcttgc ccaacaaaag
      361 cttgaccgcc tcggccgcct gcaccgccgc caccatccct gccgtaaaaa ccagcgcgcc
      421 ctgttcgtat tcctcgccgt gctccgcgtc cgcgtccggg tagacctggt ccagcaggtt
      481 ctcgccaggt aaaaccacgc acacttgccc cagccatccc ccaatcgcgc cgtgcaccaa
      541 gggcaccccc gcccgcgcgc aagccttctg cagcgttctg cgcgccggaa tgctgtccag
      601 gcagtccagc gccacctggc agccttccag cagctgcgcg gcgttctcct cagtcaggag
      661 cgcttgcacg ccctccaccc gcgccagggg gttgaccgcc tgcatgcgct gcaccgccgc
      721 cagcgccttg ggcctgccca aattcatact ggagctgagc agttggcggt tgaggttgcc
      781 ctcgctgaag ctgtccgggt ccaccgcgcg caaattgccc acgcccagcc ggccgaggta
      841 ctccagcaca aagccgccca gcccgccgca gccggccacg agcgcgcggc accccgccag
      901 gcgctcctgt tccccggggc tgatggcgag catgttgcgc aggtagcgac cgggctgcat
      961 tgcttatccc cccgcaatga ggtgcagcac ctgcacgtca tccccatccg cgatcaggcg
     1021 ccgggcgtag gcttcttccg gcacgtactc gccgttgacg cgcacgatga tatgccggaa
     1081 gacgtagccc atctcatcca gcagggcttg cacgttcagg ccgtcgcgcc agggctgctc
     1141 acgggtattt acgctaatca taggtgcttt tccacgacct ccagcagcat cggcaggtcc
     1201 atcccgattt ctttcaggtg ctccggcgtg ggcacgccgt cgttcgtcca gccgcggcgc
     1261 ttgtagaccg cgtcggtgag cagttcgaac tgcttttcgc gccaggtgcg cgtggcggcg
     1321 attttttctt ccaggctcag cgtttcgggg tccagacctt gcagttcgcg cagctgcttg
     1381 tcgtagcgtt cctgccggga gaggtactcc tccgcggtta ccggcccgag cgagcggtag
     1441 ggcgccttgt cgtgctttcg caggccgctg cccatgcgca ggttgaacac gcgctggaag
     1501 ttgtaaaccc gttcggactg gcggatgagt tcgtcgttat caattttctt gccggtcacc
     1561 gcgttgaaga tatccacgta attctggacg tgctcgggga ccttggcggg cgtgtcggtt
     1621 ttgtagttat ccgccggcac aatgtcgttc cagggcagtt tgcacaggcc gcacaagcca
     1681 aaccaggtgc ggaacatggg gtagtagaac agggcgtagg ctttatcttc gaaggttggg
     1741 atttggttgt tgaccatgtc catgaaaatc agccaggctt cgtcgtgctg ggggcctttg
     1801 ttggtgagcg cgaagccgcc ctgctgcgcc aggctctctt tgtgcaggta ttcggagtat
     1861 tccagacctt tgttttccat gccgatatct tccagcagct tggggtctgc gccaaagcgc
     1921 tccacaaaaa cctgtttcag gcggcggata cctaaacctg ccaggaggcc aaagccttcc
     1981 ccgcgagaca tctggtgcag gagttccagc gaggcagctg cgtttccgaa gcgcaaatcc
     2041 agcccgccgg tatgctccgg gccgatttgc tcacgctcat agcattccat gatgaaagcg
     2101 accgtcgtgg cgtaggaaat caggtcgatg ccgtaggtgt cgcagtagaa gttctgttcc
     2161 aggatggcct gggggtcaaa gatacccagg ttagcgccca gcccggcagc gcattcatat
     2221 tccgggccgt ccacggtcac gcactgcccg gcgtaagggc cggtcagcag cttgaagtgg
     2281 tcggcggctt tggcgcaaga catggtgcag ccgtaccagc agccgtcgtg caggccctgg
     2341 gtcaggtatt gggtgatgaa cacctcgggc gagattttca ccgcctccgg gtctccgccg
     2401 gtctggaagt tgcgcgtggg cagcaggtcg tacttgttca tcaccaggat ggtgttggcg
     2461 gtgccgattt tgcgcatgcc gttctgtttt tcgtccaggt cgtgcatttc tttgtggtac
     2521 ttgacgccga tgcggttgag cgcggcgata tcggccgggt tgttcaggtt cggtttgatg
     2581 cctttgaact tgaccaccag ggctttgatg tgcttgtcgc gcaggatggt gcccaggccg
     2641 ccgcgcgcgg cctgcttgaa gcgcacggtc ttgcggcgcg ggtcgtagaa gctaaagttg
     2701 agcatgccca tcaggacgtg cttggccgcg ctgccggagg aaacgaccga gacgtgtacc
     2761 ttgtcttcct cgctgccggc aaaacgggtg gtcaattcct ccgccagcaa atgcgagtcc
     2821 tcggggtact cctcgccttc ctcgatggag ataacgcctt cattgccgtc gatgaagata
     2881 atcaggtcct tttcggcctt gccctgcagt tccagcgcat cccaaccggc aaacttgagc
     2941 agcggcccga agtagccgcc ggcgttgctg tcaatcggga tgccggtgag cggggagatg
     3001 gcgcatacca gggtcttgcc tgtgccggaa tactgtgtga tgcctcccaa cgggccgacg
     3061 ctcatcacga tttcgttctc cggcgagtcc cagcgggtgt cgggcgtgac ggcgttccag
     3121 aggtaccaca ggtcaaagcc tttgccgccc acgaacttct ctttcatgaa cccggtgact
     3181 tctttttcct cgatgcgcag gtcgcccacg ttgatgtaga gggatttatt ggaatagccg
     3241 cgctcaattt tggcgggggt atagtggatt tgcttgagaa ttttgcccat ttttcagtcc
     3301 cctttgctct gacttaccag gatttccaac gcgccttcgg ggcaggcctt cacgcagata
     3361 ttgcaagcga tgcacttgaa gggcaccagc aggtcgtcgc tgtagaacat ggagagctcg
     3421 gagcagaaac cgacgcagcc caggcagcca acgcacttct tcctgtcaat gcgcaccacg
     3481 ccgttcttat cgcggtggat ggcgtcggcg gtgcacatat tgatgcattc gccgcattga
     3541 gtgcacataa tcgcgtggaa ggtgttgaag ttgttgttga tgcggatggc ggatttgagg
     3601 cggtcgtcag tcctgaaata tgcctgggaa caggccagct cgcacgcgcg gcagcctgtg
     3661 catatctcct gcttatgggt gaggactttc atcaggagct tcctttccta agggtgatac
     3721 gcctattcta aggtgaggcg tgctttccgt caaccagagg gggcggggcg tgttttgggt
     3781 ttgagtcaat cggaaacagc agctttactt cagcgtttcg taagggattg gttccacctc
     3841 gcgcccctgg ccggattttt cttccagctt gccgttcggg tagtagaagc gctggcgcag
     3901 ctgccaggtg agcgggccgt ggtggcggta gccctggtag gtaccccaaa actggcagac
     3961 ccggaaccag agaatgctga agaagtgtct gcccaggact ttttggcggg aggcgttttg
     4021 catgtcccgc aggatattgc gcgaactgag gcgcacaaag tccacaaagc cgaagcgttc
     4081 atgcgtgtag atgtagcgga aggcaatcgc ttcgcgccgg tagcggttgt acacgccgcg
     4141 cggggtttcg ttgtgcacgt gcacgatgac cgcctcgggg gcatagtgga ttttgtagcc
     4201 ggcctgcagg atgcgattcg cccaatccag gtcttccaga ccgggcagag tttcgttgta
     4261 ggggtgttcg agcgcgagcg cgcggcgcac ggcggcgttg gcgttgttgc agaaggcgct
     4321 gtcctgatag ggttgggtcg tatccgggaa ccagctggca aaaatctgct cctcggagta
     4381 tttggtggtc tcgccgccgc gctgcttgcc gtagaccagc gcgatagcgg ggtcggcgaa
     4441 aggcgccagc agcttttcca gccagtccgg gtagacgggg tagacatgtg cgctggcgaa
     4501 gaccaggaac tccccgttgg cggcctgaat accgcggttg agcgagcgcc cgaaagtgaa
     4561 ttcctcaggg gcgatgtgga cgagtttggc ggagaagcct tgcgcgattt ccacggtccg
     4621 gtcgctggag ccagagtcga ccaggataat ttcggcgtct tgcacgcttt ggcgggcgat
     4681 gccttccagc aggcgggcga tatgcttttc ttcgttgtaa gagcggatga tgatggaaca
     4741 gcggggctgg gtcaagaggc acctgttttg tgttttttac cgggtgaagt actcgccgga
     4801 agcttattat acattgtttc cgctggctgt ccgcgcgcgc gggcttgcct tgtcagcgaa
     4861 cccgacagga catagtcgta gtcgcaggtc cggtggagcg gcagcgcaac ccgacagaaa
     4921 gtggccgtag gtcgggtggg gcggcggcgc aacccgacag aacatgatcg taggtcgggt
     4981 gaagcggcag cgcaacccga cagaccgtgg tcgtaggtcg ggtggagcgg cagcgcaacc
     5041 cgacagaacg tagtcgtagg tcgggtggag cggcagcgca a
//
LOCUS       DAOQAD010000127         5081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_20811_length_5081_cov_6.257262, whole genome shotgun sequence.
ACCESSION   DAOQAD010000127 DAOQAD010000000
VERSION     DAOQAD010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20811_length_5081_cov_6.257262"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..776)
                     /locus_tag="PKL60_08115"
     CDS             complement(183..776)
                     /locus_tag="PKL60_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96136.1"
                     /translation="MKTTGFTSDMKGSESMRLASWEAFIAVHPQRVLVLGNGGSGKST
                     LAQELSLRLKLPIVHLDRIFWLPGWQSVSPELFDERLREEINRETWIIDGNYTRTLEL
                     RLSRSQAVVFMDMPRMVCLAGVLARYFSFRGRTRPDLTPGCPEKVDWEFLRWIWNFNR
                     SHRAAYLERFASLDQPVVILRSRKAAARFLSGLENAA"
     gene            953..1285
                     /locus_tag="PKL60_08120"
     CDS             953..1285
                     /locus_tag="PKL60_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNW96137.1"
                     /translation="MEPNSNPLLSERDAARMALTFKGLADPTRLKILSVLLGGEIRVS
                     DLAERIQTSQTAVSHQLRVLRDLRFVSSRREGTQIFYRIDDEHIEDLFNKAFAHGKHL
                     SDREGEPR"
     gene            1282..3444
                     /locus_tag="PKL60_08125"
     CDS             1282..3444
                     /locus_tag="PKL60_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNW96138.1"
                     /translation="MKSIVIPLQGLDCVDCARGIEASLKRLPGVQDARLDFTRAELTV
                     EGDADPELLRESIRQLGYIPLAAHAKGPANLSFAGFWKTLSTNRTLRATLPGLALLAG
                     SLYLPRLGVNGTWRVVIQLAALLAAGFPIFLSGLRSLFLDRALTINFLLTLAVLGAVL
                     INETPEAFIILILFHLSEAMESYTNDHARAVLTEFADLAPKTAVRLAESGEEIVPIED
                     LALDDVIIVRAGERFPMDGVIVEGNSEINQAPITGESRLIPKGTGDPVISGTINGQGV
                     LKVRVSALARDTTVQRIVNLVLDARAVKARQEKFIDRFASIYTPVVVAVAVLTALLPT
                     LLFGQPLWNSAGGYGWLHRALSLLMIGCPCALVVSTPVTIISGLTRAARAGVVFKGGV
                     YLESLGQSKLAAFDKTGTLTLGKPVIRTVKAVDCEGTEQCEPCTDLLALASSLEYYST
                     HPLGQAVMSAANARSLQTRYGPAEKLESLNGRGQQGYVNGKLATVGSLPLFDAEHEVP
                     AELRQETLSAETQGQTTMLVCDGERVRGFLGAQDSLRPEAEAVIAALRQRGLRTVMLT
                     GDNAETAEQAAGLLGLDETHAGLLPDEKYKLLGELKQKYGQVLMVGDGSNDSPALASA
                     DIGIGMGGAGNAQVLETADVVLMQDELSQLPFAFDLSRYVNRLIRQNITISLGVKASV
                     AVLALLGLTPLWVAVLADIGITLLVTLNGMRAMRFQPA"
     gene            complement(3461..4684)
                     /locus_tag="PKL60_08130"
     CDS             complement(3461..4684)
                     /locus_tag="PKL60_08130"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNW96139.1"
                     /translation="MNARAAPGFLPGIFLFLVLCLGLSACSPQDAPPPLTPAALRQVS
                     PTPGPAQRQTQAAAAATPTPIPSPTPWAYATYTPTLPPADLAAAGINPLTGLPAQEKS
                     LLEERPVLVKIANWPQTLRPAAGLNQADLVFEYYIGFQMNHLTAVFHGRDCERVGPLA
                     PARMVDARLAEHYQGIVAYASADSTVEKIFAEVLPERAFARGFAPCPAICTYTDTTGE
                     NTFVDTAALRETAGEEEELKLAAERPGFTFDPRLSHWDADAETFSLQYADFSVMEWRF
                     DSSTDQYVLYQDFAGNGSIDITQSTDRTDGSRIAFENVLVLFANYIEYNPTMYDVDFR
                     EGDPNQRALLLRDGKLIHGTWSSADPFSPINLFSTGGAPLALKPGRTWIVFVGTNSKT
                     TQTSSAAWELIFSLQ"
BASE COUNT         1022 a         1464 c         1568 g         1027 t
ORIGIN      
        1 cgcgatgacg agaacaggca gatcgccgcg cccccttctt tcatttctga cgcgccatta
       61 ctcccagcag cgggggctcg cgatgacaag aacagacaga tcgccgcgcc cccttctttc
      121 atttctgacg cgccattact cccagcagca ggggctcgcg aggacaaaat aaaggctgcg
      181 aatcaggcag cgttttcgag cccagacagg aaacgcgccg cggcttttct gctgcgcaga
      241 ataaccaccg gctggtccag gctcgcgaag cgctcgaggt aagctgcgcg gtggcttcga
      301 ttgaagttcc agatccagcg caggaactcc caatccactt tctccgggca gcctggcgtg
      361 agatccggtc tggtgcggcc gcgaaagctg aagtatcggg ctagtacccc tgccaggcag
      421 accatacggg gcatgtccat aaaaaccaca gcctggctgc ggctcaggcg cagttcgagc
      481 gtgcgtgtgt aatttccgtc aatgatccag gtctccctgt ttatttcctc tcgcaagcgt
      541 tcatcaaaca gctccgggct cacggactgc caacccggca gccaaaaaat gcggtcaaga
      601 tgcacgatcg gcagcttgag cctgaggctg agttcctgcg ccagtgtgct tttgccgctt
      661 ccgccgttgc caagtaccag cacccgctgg ggatgaactg cgatgaaagc ctcccaactg
      721 gccaggcgca tgctctcgct gcccttcata tcagaggtaa agccggttgt tttcacctcc
      781 ggattttacc attgagcacg gcagggatta gcgcggcccc gaataatttt cggctgcttg
      841 ggatggaaat tgcccgcaaa tggtcatttc ctccaattgc ccttgcgctg aaaaccgaaa
      901 cgcgtataat atgcttatgt gttcatataa gcatattagg aaggcgtaaa acatggaacc
      961 aaactcgaat cctctccttt ctgaacgcga tgccgctcgc atggcgctga cctttaaggg
     1021 cctggctgac ccgacccgcc tgaaaatcct ttccgtcctg ctgggcgggg aaatccgcgt
     1081 ctcggacctg gcggagcgca tccaaaccag ccagaccgcg gtctcgcatc aactgcgcgt
     1141 cctgcgggac ctgcgctttg tctccagccg ccgcgagggg acacaaatct tctaccgcat
     1201 cgacgacgaa cacatcgaag acctgttcaa taaagctttt gcccacggca aacatctttc
     1261 agaccgggaa ggggagcctc gatgaaatcc attgtcattc ctctgcaagg cctggactgt
     1321 gttgactgcg cccggggtat cgaagccagc ctgaagcggc tgcccggcgt gcaggacgcg
     1381 cgcctggact tcacgcgcgc ggagctgacg gtggaaggcg atgccgatcc cgagctcctg
     1441 cgcgagagca ttcgccagct tggctacatc ccgctcgcgg cacacgcgaa aggacccgcg
     1501 aacctgagct tcgccggttt ctggaagacc ctcagcacca accgcacctt gcgcgccacc
     1561 ttgcccgggc tggccctgtt ggccggctcg ctctacctgc cgcgccttgg cgtgaacggc
     1621 acctggcgcg tcgtcatcca actcgctgcg ctgcttgcgg caggctttcc catttttctc
     1681 agcgggctgc gcagcttgtt tctcgaccgc gcgctgacga ttaactttct gctgacattg
     1741 gctgttctcg gcgcggtgct catcaacgaa actccggaag cttttattat cctgatcctg
     1801 tttcatttat ccgaggcgat ggaaagctac accaacgacc acgcccgcgc tgtgctgacg
     1861 gaattcgcag acctggcgcc taaaacggca gtgcgcctgg ctgaatccgg ggaggagatt
     1921 gttcctatcg aagacctggc gctggacgat gtcatcattg tccgcgctgg cgaacgcttc
     1981 ccgatggacg gggtaatcgt cgaaggaaac agcgagatta atcaggcgcc aatcaccggt
     2041 gagagccggc tgatccccaa agggacgggc gacccggtta tttccggcac tatcaacggg
     2101 cagggggtat tgaaagtgcg cgtgagcgcc ctggcgcggg atactaccgt gcaacgcatc
     2161 gtgaaccttg tgctggacgc gcgcgcggtg aaggcccggc aggaaaaatt catcgaccga
     2221 ttcgcgtcca tttatacccc tgtcgtcgtc gcggtggctg tgctgaccgc gctgctgcca
     2281 accctccttt tcgggcagcc gctgtggaac agcgccggcg gatacggctg gctgcaccgc
     2341 gcgctctcgc tgctgatgat cggctgcccc tgcgcgctgg tggtcagcac gccggttacc
     2401 atcatcagcg ggctcacccg cgccgcgcgt gccggcgtgg tatttaaggg tggcgtttac
     2461 ctcgagagcc tcggccagtc gaaactggca gcttttgaca agactggcac gctaaccttg
     2521 ggcaaacccg tcatccggac cgtgaaagcg gtcgattgcg aaggtacgga gcagtgtgag
     2581 ccttgcacgg acctcctggc gttagccagt tcgctggagt attacagcac gcatcccctg
     2641 gggcaggcgg tgatgagcgc ggcaaacgcg cgcagcctgc aaacccgcta tggacccgca
     2701 gaaaagcttg aatcgctgaa cggcagaggg cagcagggct atgtgaacgg aaaactggct
     2761 acggtgggca gcctgccctt gttcgacgcg gaacatgagg tgccggctga attacggcag
     2821 gaaacgcttt cagctgagac ccaggggcaa accaccatgc tggtctgcga tggcgaacgc
     2881 gtgcgcggtt ttttgggcgc gcaggacagc ctgcgcccgg aagctgaagc ggtcatcgcg
     2941 gcgctcaggc agcgcggact gcgcaccgtc atgctgaccg gggataacgc tgagacagcg
     3001 gaacaggcag ccggcttgct cggtttggat gaaacgcatg cggggctgtt gcccgatgaa
     3061 aaatataaac tgctgggcga actcaaacag aaatacggcc aggttttgat ggtgggggac
     3121 ggcagcaacg acagcccggc tctggccagc gcggatatcg gcattgggat ggggggcgcc
     3181 ggcaacgccc aggtgctcga gacggcggat gtggtcttga tgcaggatga gctcagccag
     3241 ctgcccttcg cctttgacct ctcgcgctat gtcaaccgcc tcatccggca gaacatcact
     3301 atcagcctgg gcgtaaaggc ttcggtggcg gttttggcgc tgttgggatt gacgccgctg
     3361 tgggtggcgg tgctggcgga tatcggcatt accctgctag tgaccctgaa tgggatgcgc
     3421 gcgatgcgct ttcagccggc ttagctcagg ttttccggcg ctactgcagg gagaaaatca
     3481 gctcccaggc ggcgctgctg gtttgggtgg tcttgctgtt ggttcccacg aagacaatcc
     3541 aggttctacc cggtttgagc gcgagggggg caccgccggt gctgaataaa ttgatcgggc
     3601 tgaaggggtc agcggaagac caggtgccgt ggatgagttt gccgtcgcgc agcagcagcg
     3661 cgcgctggtt ggggtcgccc tcgcgaaagt ccacgtcgta catcgtcgga ttgtattcga
     3721 tataattggc aaacagtacc agcacattct caaaggcgat gcggctgccg tccgtacggt
     3781 cggtggattg cgtaatatcg attgaaccgt tcccggcaaa atcctgatag agcacatact
     3841 ggtcggtact gctgtcgaag cgccattcca ttacggaaaa atccgcgtat tgcaggctga
     3901 aggtttcagc gtccgcatcc cagtgcgaga ggcgcgggtc aaaggtgaag ccgggccgtt
     3961 ctgccgccag ttttaattct tcttcttcgc cagccgtctc gcgcaaagca gcggtgtcta
     4021 cgaatgtatt ttcgccggtg gtatctgtat aggtgcaaat agcagggcag ggagcgaaac
     4081 cgcgcgcgaa agcgcgctcc ggaagtacct cagcgaaaat cttttccacg gtgctgtccg
     4141 cgctggcata ggcgacgatg ccctggtagt gttccgccag gcgcgcgtct accatgcggg
     4201 caggcgcgag aggacccacg cgctcgcaat ccctgccgtg aaagaccgct gtaaggtggt
     4261 tcatctgaaa gccgatgtaa tactcaaaca ccaaatccgc ctggttaagc ccggctgccg
     4321 ggcgcaaggt ctgcggccaa ttggcgattt tcaccagcac cggcctttcc tccaacaggg
     4381 atttttcctg cgcgggcaga ccagtgagcg gattgatgcc ggctgccgcc agatccgcgg
     4441 ggggcaaggt gggggtatag gtggcgtagg cccagggcgt gggcgatggg atgggtgtgg
     4501 gcgtcgcggc ggcagcggcc tgggtctggc gctgggctgg gccgggggtg gggctgacct
     4561 ggcgcaaggc ggcgggggtg agaggcggcg gtgcgtcttg cggcgaacaa gcggacagac
     4621 cgaggcagag caccaggaat aggaatatgc ctggcaaaaa tccgggggcg gcgcgtgcat
     4681 tcatgcctgg attttaacat gttcagcgtg cttgtgttca gggatgacaa gaagcctggc
     4741 gaagccgcag ggattcattt gcccggagct gcatttcttg tccaacgcat gccaatattg
     4801 acgaatgacg attgcaattc cttgtttgtc ggttcaaaag ggctgaaaaa aaggaaaagc
     4861 ctgctcgagg acactccaca cgagactttt acccgttgtt aacctggaat tgaaggctcc
     4921 ttaaaccccc tatgttaagc ttagacaaac ttgaaagata aagaagagaa aagaggaata
     4981 tgcgtaagtt tttggttgtt atcgttttac tcacggctac ggttctgagc gcgtgcggcg
     5041 gcaccagcgc atctggttcc ctcagcctcg ccggctccac c
//
LOCUS       DAOQAD010000128         4983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_21402_length_4983_cov_6.325081, whole genome shotgun sequence.
ACCESSION   DAOQAD010000128 DAOQAD010000000
VERSION     DAOQAD010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21402_length_4983_cov_6.325081"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PKL60_08135"
     CDS             complement(<1..459)
                     /locus_tag="PKL60_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96140.1"
                     /translation="MSIRARFTLLYNVILLVTLLVFGAALYGIQAGSTLNSLKKDLTY
                     SSDAVAINLVPMLLANLPGLGPYTSADIKTFESLSDADVIKNLREREIVRVLNAEGEL
                     IAAPYGVGEGGLPYSAQGYSELQKGNIWWESVRLNEKLLVLNRPVMVDGKL"
     gene            complement(459..1136)
                     /locus_tag="PKL60_08140"
     CDS             complement(459..1136)
                     /locus_tag="PKL60_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNW96141.1"
                     /translation="MPKILITDDDQKLLKMLKRTLTYENMDVLTASTGVEALEIVSQQ
                     KPDVLILDWMMPEMDGLELVQRLRDMNNRLPVLMLTARDAIEDRVDGLESGADDYLVK
                     PFAPSELVARVHALLRRIETRPDDTAVQYADLRLDPIQHEAWRGSRLLNLTVTEFNLL
                     HYFLRHPRQVLERSQILTEVWGYDFGGDSNVLEIYISYLRKKIETEGESRLIQTVRGI
                     GYVLREE"
     gene            1488..2234
                     /locus_tag="PKL60_08145"
     CDS             1488..2234
                     /locus_tag="PKL60_08145"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNW96142.1"
                     /translation="MESEEKPFPLIYAHRGASTLAPENTLAAFQLALLAGADGIELDV
                     MLSRDKELVVIHDATVDRTTDGTGKVADLPMAALRELDAGARFGGDFRGEKLPTLAEV
                     FEAVGGKMRINVELKNYAHPLDDLTSRVIALTERFQLEDSVLLSSFNPINLSKARRLN
                     PNICRGLLVSPGDRLMLGGVGRFFPYHALHPYYEDVAPELTADLHARGKQLNTWTVDD
                     PEALLRLRAWGVDGIICNNPSAARAVLEGR"
     gene            2234..4459
                     /locus_tag="PKL60_08150"
     CDS             2234..4459
                     /locus_tag="PKL60_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNW96143.1"
                     /translation="MKIRRALGLFLALSLLLSVLPIPARANSPSQAESLLADMSPAEK
                     VGQLFLITFNGSNISEGSLLWELISTYHIGGFVLRADHDNFSGTDTLVSAYNLIAAMQ
                     SAAWDKTQPSPDTNLGGDLPAYVPLFIGIEQNGGSSNQILSGLSDQPNPMTLGATWSP
                     SLAEQAGQVLGSELSALGFNLFLGPTLDVAETINMDTAAYSGADIYGADPYWVGEIGR
                     AYIAGLHAGSGKRLSVIARHFPGLGSADRPPDLEVATVRKSLEQLKQIELAPFFAVTS
                     AEDSSAQADGVMVSHIRFQGFQGNIRATTRPISFDATALAQLLAVEPLASWRAAGGLT
                     VSHSLGSKAVRSFFDPTGQSFDANTIAGTAFIAGNDLLYLNDFSNASDATPYATILQT
                     LDYFSGKYEEDPAFAQRVDEAVLRILNAKLRLYGEFSLEKVIPARAGLDQIGSSSEFA
                     FNIAREAVTLISPSQDYLNSLLPSPPDTYEYMVIFSDQRARKQCSTCPPVYELSANMF
                     QNTLMKLYGQAGSNQLLQNRLSSYSFSQLTELLDRKTELSDPYLPDNLKRATWVIFNI
                     QDLDAELPESSALQRILSERLDLIRDKKIIVFAYDAPYYLDATEISKLSAYYALYNTS
                     QSSVDVAARVLMQEIQPSGALPISLPAVGYDLIFETSPDPNQVIPITLVTPREMPTPE
                     AGTAAAEATPMPLFAVGETVRIQAGVIATTTNTLCRTAQLCSSPSAWRAIRRLSVSLR
                     R"
     gene            4399..>4983
                     /locus_tag="PKL60_08155"
     CDS             4399..>4983
                     /locus_tag="PKL60_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96144.1"
                     /translation="MQFTIRLAGDQAIIGQPQAVTKDGFASVEYRIERDGIFEVTASS
                     EPATTSGTLILNTQGGLAQLIMPTATPTLAPSPTPLPTTTPEASPTPEANAAADGSYP
                     KMDDWLLTIMVMSLGFGAAFASGQFWWGSRLWGVRAGVCALIGGFAAYLMLMLGLPSL
                     EALVREGGTWFVVEVALSGMLFGWLSALVWWYAKK"
BASE COUNT         1011 a         1524 c         1399 g         1049 t
ORIGIN      
        1 gagttttcca tccaccatga ccggacggtt cagtacgaga agtttttcat tcaggcgcac
       61 gctttcccac caaatattcc ccttctggag ctcggagtag ccctgggcgc tgtaaggcag
      121 accgccttca cccacgccgt aaggcgcggc aatcagctca ccttcagcgt tcagcacgcg
      181 tacaatctcg cgctcccgca ggtttttgat gacatcagcg tcggaaaggc tctcgaaggt
      241 cttaatgtcc gcggaggtat aaggtcccag acccggcagg tttgccagga gcattggaac
      301 cagattgatg gcaaccgcgt cgctgctgta cgtaaggtct ttcttaaggc tgttgagcgt
      361 gctgccggct tggatgccgt acagagccgc gccaaatacc agcagcgtga ccagcaggat
      421 aacattatag agcagggtga agcgcgcccg gattgacatt attcctcacg aaggacgtag
      481 ccaatcccgc ggacggtctg aatcagccgg ctttcccctt ccgtttcgat tttcttgcgc
      541 aggtagctga tatagatttc gaggacgttg ctgtcgccgc caaagtcgta gccccacact
      601 tccgtcagga tttggctgcg ttccaacacc tggcgggggt gccgcaagaa gtaatgcagc
      661 aggttaaact cggtcactgt caggttgagc aggcgcgagc cgcgccaggc ttcgtgctga
      721 atcgggtcca ggcgcaggtc ggcgtattgc actgcggtgt cgtcggggcg cgtttcaatc
      781 cggcgcagca aagcgtgcac gcgcgccacc agctccgagg gcgcaaacgg cttcaccaga
      841 tagtcatccg cgccgctctc caggccgtcc acccggtctt caatcgcgtc gcgcgcggtc
      901 aacatcagca caggcaggcg gttattcatg tcgcgcaggc gctggacaag ctccaagccg
      961 tccatctccg gcatcatcca gtccaggatc agcacatcag gtttttgctg gctgacgatt
     1021 tcaagcgcct ccacgccggt cgaggcggtg agcacgtcca tgttttcgta tgtaagtgtt
     1081 cgtttcaaca tcttcagtag tttttggtca tcgtccgtaa tcagtatttt tggcatcgcg
     1141 catcatcctg aatttgtctg tgattcttag atatataaca caatataccc gctccggctg
     1201 tgaaaattct gtgactgctt ttttcacagc tttctccaag ctattttttg gctgaatcac
     1261 agaatcagcg cttactatag aaacgtcaga acgacaggat gcagttcctc ctcaaaggaa
     1321 ccgcagcagc cagcatggat aactgactgc aaaaaaaaac agacgaacga tttcgatctt
     1381 tctcctccag atataagcag cggtaacgac gggccgctgc ttatatttta acaatgagct
     1441 gccatgggct ttagaacctt ccgcttcgca tgctataatt tattccaatg gaatccgaag
     1501 agaagccttt tcccctcatc tacgcccaca gaggagccag tacgcttgcg cctgaaaata
     1561 cgctggcagc ctttcaactc gcactgctcg cgggcgccga cggcatcgag ctggatgtga
     1621 tgctcagccg cgacaaagag ctggtcgtca ttcacgacgc gacggtcgac cgcacgacgg
     1681 acggcactgg caaagttgca gatctgccga tggctgccct gcgggagctt gatgccggcg
     1741 cgcgctttgg gggggatttc cgcggcgaaa aattgcccac gctggcagag gtttttgaag
     1801 ccgtgggcgg aaaaatgcgc atcaacgtgg aactcaaaaa ctacgcccac cccctggacg
     1861 accttaccag ccgcgtgatt gcgctcacgg agcgttttca gctcgaagat tccgtactgc
     1921 tctcctcttt taatccgatt aacttgagca aagcccggcg cttgaatccg aatatctgcc
     1981 gcgggctgct cgtttccccc ggggaccggc tgatgcttgg cggtgtcggg cgcttttttc
     2041 cctatcacgc cctccacccg tactacgaag acgtcgcgcc tgagctgacc gctgacttgc
     2101 acgcccgggg aaaacaattg aacacctgga ctgtagacga ccccgaagcg ctgctgcggc
     2161 tgcgcgcttg gggcgtggac ggcatcatct gcaacaaccc ctccgccgct cgcgccgttt
     2221 tggaaggcag ataatgaaaa tccgccgcgc gctcgggctt tttctggcgc tcagcctgct
     2281 gctttcggtg ctgcccatcc cggcgcgggc caactcgccc tctcaggcgg agagtctgct
     2341 ggcagacatg agcccggcgg aaaaagtcgg gcagctcttc ctgattactt tcaacggcag
     2401 caacatcagc gagggctcgc ttttgtggga gctcatcagc acctatcata tcggcggctt
     2461 tgtgctgcgc gcggaccacg ataacttctc cggaacagac acgctggttt cggcctacaa
     2521 cctgattgcc gccatgcaaa gcgcggcctg ggataaaacc caaccctcgc cggacactaa
     2581 cctgggcgga gacctgccgg cttacgtgcc gctctttatc ggcatcgaac agaatggggg
     2641 tagttccaac cagattttga gcggcctgag cgaccaaccc aatccgatga cgctcggagc
     2701 tacctggtcc ccttcgctgg cagagcaagc cgggcaggtg ctcgggagcg aactttccgc
     2761 gcttggcttt aatctgttcc tggggccgac cctggacgta gcggagacta tcaatatgga
     2821 taccgcagcg tactccggcg cggatatcta cggagccgat ccgtactggg tcggagaaat
     2881 tggccgggcg tacattgctg gcttgcacgc cggcagcggc aagcgcctga gcgtaatcgc
     2941 gcggcacttc cccgggctgg gcagcgccga ccggccgccg gacctggagg tcgccacagt
     3001 ccgcaagtcg ctcgaacagc tgaaacagat tgagctcgcc ccgttcttcg cggtgacttc
     3061 cgctgaagat tcaagcgctc aggcggacgg cgtgatggtt tcccatatcc gctttcaggg
     3121 ttttcagggc aacattcgcg ccacaacccg ccccatcagc tttgacgcga ccgcgctggc
     3181 acagctgctg gctgtggaac ctctggcaag ctggcgcgcc gccggcgggc tgaccgtcag
     3241 ccacagtctg ggcagtaaag ccgtgcgctc tttctttgac ccaaccggcc agagcttcga
     3301 cgcgaatacc atcgctggca ccgcgtttat cgccggcaat gacctgctct acctgaatga
     3361 cttctccaac gcgagcgacg caaccccgta tgcgaccatc ctgcagacgc tggattactt
     3421 tagcgggaaa tatgaggaag accctgcctt cgcgcagcgc gtggatgaag cggtcctgcg
     3481 catcctgaac gccaaattgc ggctttatgg cgagttctcc ctcgaaaagg tcatcccggc
     3541 ccgcgccgga ctggaccaga ttggaagctc ttccgagttt gccttcaaca tcgcccgtga
     3601 agctgtcacg ctcatatctc ccagccagga ttacctgaac agcctgcttc ccagcccgcc
     3661 cgacacttac gaatacatgg tcatcttcag cgaccagcgc gcccggaagc agtgcagcac
     3721 gtgcccgccc gtttacgaac tctctgccaa catgttccag aacacgctga tgaagctgta
     3781 cgggcaggct ggcagcaacc aactcctgca aaaccgcctg agctcctaca gtttcagcca
     3841 gcttacagag ttgctggacc ggaagaccga gctctccgac ccgtacctgc cggataatct
     3901 caaacgcgcg acctgggtga ttttcaatat ccaggatttg gacgcggagc tgccagaatc
     3961 atccgcgctg cagcgcatcc tttcggaacg cctggacctg atccgtgata aaaaaatcat
     4021 cgtttttgca tacgacgcgc cgtactacct ggacgcgacc gaaatttcca agctatccgc
     4081 ctattacgcg ctctacaaca ccagccaatc cagcgtggat gtggccgcgc gcgttttgat
     4141 gcaggaaatc cagccttccg gcgcgctgcc catctctttg cctgccgtcg gatacgactt
     4201 aatcttcgaa acctccccgg atccaaacca ggtcatcccg attactttgg tcaccccgcg
     4261 cgaaatgccc acgcccgaag ctggaacggc ggccgcagag gccaccccga tgccgctgtt
     4321 cgcggtgggc gagacggtcc ggattcaagc tggcgtgatt gcgaccacaa ccaacacctt
     4381 gtgccggacg gcacagttgt gcagttcacc atccgcctgg cgggcgatca ggcgattatc
     4441 ggtcagcctc aggcggtgac taaagacggc tttgcctcgg tggaatatcg gattgaacgg
     4501 gatggaattt ttgaggtaac cgccagcagc gaaccggcga ctacctccgg aaccctcatc
     4561 ctgaacacgc agggcggtct ggcgcagttg attatgccta cagccacgcc tacgcttgcc
     4621 cccagcccaa cccccctgcc gacaaccacg cccgaggcaa gccccacccc tgaagcgaat
     4681 gcggcggccg acggcagcta cccgaagatg gatgactggc tgctgaccat tatggtgatg
     4741 tctttgggtt ttggcgcggc tttcgccagc gggcaattct ggtggggatc caggctgtgg
     4801 ggtgtgcgcg cgggggtttg cgccttaatc ggggggttcg cggcttacct gatgctcatg
     4861 ctgggcttgc cctcgctgga agcgttggta cgtgagggcg gcacatggtt tgtggtcgag
     4921 gtggcgctga gcgggatgct gtttggctgg ttgagcgcgc tggtgtggtg gtacgcgaaa
     4981 aaa
//
LOCUS       DAOQAD010000129         4796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_22642_length_4796_cov_5.594389, whole genome shotgun sequence.
ACCESSION   DAOQAD010000129 DAOQAD010000000
VERSION     DAOQAD010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22642_length_4796_cov_5.594389"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="PKL60_08160"
     CDS             <1..2083
                     /locus_tag="PKL60_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNW96145.1"
                     /translation="AQAQGGRPQTFGGLKIASCPTHTAIACVLKTILPEMPFIGKISA
                     LRTRMNMTPEQPDWLAQILHFGSADYLSNPRPRLGELVRLRLLTPLGAPIEQVVLRTI
                     PNGEQQFTDMRPGPEQAGMQAWEADLRVNEPRVPYRFAIRTREVVWWLNAVGIGQGVP
                     FSLFDFKLLADTPAIPWLSGSVFYQIFPDRFENGDPTNDPHHEALPYRNWRRDTLPWG
                     QPAPREAGLLPFYGGDLAGITQRLDYLERLGVNAVYLNPIFSAYTNHRYDVADYEHVD
                     PVLGGDAALQTLSEAFAQRGIRYILDIVPNHCGIGHPWFQAAQADPNSPEREYFFFGE
                     SPEDYVSWLGFGSLPKLNYASARLRELMYRGEDSVMRRWLRPPWNASGWRVDVGNMLG
                     RQDAQQLDREILPEIRRAVKETNPEAYLVAENFFEAASQLQGEGWDGVMNYSGFTDPL
                     LHWLRPYQQDAVHWQGKLGGSERWPTELVLRTWQEHLAAVPWAIALQQLNLVDSHDTA
                     RILSELGGNKALSMLAAVLQFCFPGVPCLYYGDEIGLRDQEGFGSRACMPWDESKWDQ
                     ELFTFYRKLIALRKSSRVLAEGAFQILFGDADSLLFQRVLGTERVLVAAKRSQDAAPA
                     RALRLPQAGFGEKEMFRGLLGGGQAKAEGGVLDLPAMPQGAEIWLPDQADAFQPETSP
                     LSARNARFPTR"
     gene            2147..3151
                     /locus_tag="PKL60_08165"
     CDS             2147..3151
                     /locus_tag="PKL60_08165"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNW96146.1"
                     /translation="MDMLSARVPENTSAWGGTVPDLSICILTLNAKAYLRECLLSIAE
                     HAPLGFARGQASAARGNGMGQALTEEVPSFEYEIIVADNGSDDGTLEMLAEEFPYVRV
                     IRNNANIGFSKPVNQMLAAARGEFFLLLNPDTLLIEDLFTPMLDWMRANEGVGIAIPK
                     VLNADGSFQKQSRRGEARPAEVFGYFLWLGRLFPRNRALNGYLQSWLPEDEIAEVKAV
                     SGSCMFIRRAAWEQIGEFDERFFAYQEDSDYCLRARKAGWKVMYVPIARIVHYGGEGG
                     SKVRPANSILAWHRSYFLYYRKHFAREYFFLFNWFFYLMMGAKLVLALLKNLFRQLIN
                     "
     gene            complement(3333..4499)
                     /locus_tag="PKL60_08170"
     CDS             complement(3333..4499)
                     /locus_tag="PKL60_08170"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNW96147.1"
                     /translation="MIENFRYYNPTQIFFGSGTIAKINKLIPRKAKVMVLYGGGSIKK
                     NGVYEQVMAALKKREVLEFGGIEANPDYDTLMRAVRKIQKKHVNFLLAVGGGSVIDGV
                     KFASLAALYPGKDPWDILTGGGLSQAKEALPFGTVLTLPATGSEMNSNAVISRRSIQE
                     KLSFGSDLVFPRFSILDPETTYSLPQKQIRNGLVDAFVHVFEQYLTYPVGALVQDRQA
                     EAILMALRDHAPDALRVPPDYNARANFMWAATNALNHTLNRGVPEDWATHNIGHELTA
                     LYGLDHAETLAAVLPWLLWYKRKQKQAKLLQYGYRVLGIKTQKYDDRIDQTIEATAAF
                     FHSLGMPTTLTAYGIDPEEAAQKVQARMESHQWTFGEHQDISGKDAAAILRLSR"
BASE COUNT          898 a         1344 c         1558 g          996 t
ORIGIN      
        1 tgcgcaagct cagggcggca ggccccaaac ctttggcggc ttgaaaatcg caagctgccc
       61 tacgcacacc gcgatcgcgt gcgtattgaa aacgattctc cctgaaatgc cattcattgg
      121 taaaattagc gctttgagga cccggatgaa catgacccct gaacaacccg actggctggc
      181 gcaaatcctg cattttggca gcgccgatta cctttccaac ccgcgcccgc gcctgggtga
      241 gctggtgcgc ctgcgcctgc tcacgccttt gggcgcgccc atagagcagg tggtgctgcg
      301 caccatcccc aacggcgagc agcagttcac ggatatgcgc ccgggcccgg agcaggccgg
      361 catgcaggcc tgggaagcgg atttgcgcgt gaacgagccg cgcgtcccct accgtttcgc
      421 catccgcacg cgggaagtgg tctggtggct gaacgccgtc ggaatcgggc agggcgtgcc
      481 gttctcgctc ttcgatttca agctgctggc ggataccccc gccatcccct ggctttccgg
      541 ctccgtcttc tatcaaatct tcccggaccg ctttgagaac ggcgacccaa ccaacgaccc
      601 gcatcacgaa gcgctgcctt accgcaactg gcgcagagat accctcccat ggggccagcc
      661 cgccccgcgc gaggccgggc tgctgccttt ttacggcggc gacctggcgg gcatcaccca
      721 aaggctggac tacctggaac gcctgggcgt gaacgcggtc tacctgaacc cgattttcag
      781 cgcctacacc aaccaccgct acgacgtggc cgattacgaa cacgtggacc cggtgttggg
      841 gggcgacgcg gctctgcaaa ccctgagcga agctttcgcg cagcgcggta tacgctatat
      901 cctggatatc gtccccaacc actgcggcat cgggcacccc tggttccagg ccgcccaggc
      961 cgaccccaac agcccggagc gggagtactt cttctttggg gagagcccgg aggactacgt
     1021 ttcgtggctg ggttttggct ctcttcccaa gctgaactat gccagcgccc gcctgcgcga
     1081 actgatgtac cgcggcgagg acagcgtcat gcgccgttgg ctgcgcccgc cctggaatgc
     1141 cagcggctgg cgcgtggacg tgggcaatat gctcggccgg caggacgccc agcagctgga
     1201 ccgggaaatc ctgccggaaa tccggcgggc agtgaaagaa accaacccgg aagcctacct
     1261 tgtggcggaa aacttcttcg aggctgcctc ccaactgcaa ggcgagggct gggatggggt
     1321 gatgaactac tccggcttta ccgacccgct gctgcattgg ctgcgtccct atcagcaaga
     1381 cgcggtgcat tggcagggca agctgggcgg gagtgaacgc tggccgaccg aactggtttt
     1441 acgcacctgg caggaacatc tggcggctgt gccctgggcg attgccctgc agcagctcaa
     1501 tctggttgac agccacgata ccgcccgcat cctgagcgaa ctgggtggga acaaagccct
     1561 gagcatgctg gctgccgtgc tgcagttctg cttcccgggc gttccgtgcc tgtattacgg
     1621 cgacgaaatc gggctgcgcg accaggaagg ctttggcagc cgcgcctgca tgccttggga
     1681 tgaaagcaaa tgggatcagg aactgtttac tttttaccgg aagcttatcg ccctgcgcaa
     1741 aagcagccgc gtgctggcgg aaggagcttt ccagattctc tttggggatg ccgacagcct
     1801 gcttttccag cgagttttgg gcaccgagcg cgtgttggtg gctgccaagc gttcacagga
     1861 tgcggcgccg gcgcgcgcgc tcaggctgcc gcaggcgggc tttggcgaaa aggaaatgtt
     1921 ccgcgggctc ttgggcggcg ggcaggcaaa ggcggagggc ggcgttttgg atttgccggc
     1981 aatgccgcag ggcgcggaaa tttggctgcc ggatcaggcg gacgctttcc agccggaaac
     2041 ctcccctctc agtgcaagaa atgcgcgttt cccgacccga taaacgattg tataatcgtc
     2101 atagaagcaa atcaaacatg caaaaaaccg aaggggcatt gttctgatgg acatgctgag
     2161 cgcgcgagta cctgagaata cctcggcttg gggcggaacc gttccggacc tctccatttg
     2221 tattctcacc ctgaacgcca aagcctacct gcgggagtgt ttgctttcca tcgctgaaca
     2281 cgcgccgctt ggtttcgcgc gagggcaggc ttcggctgcg cgtgggaacg gcatggggca
     2341 agccctgacg gaagaggttc cctcgtttga atatgagatt atcgtcgcgg ataacggctc
     2401 ggatgacggt acactggaga tgctcgcgga agaattcccg tatgtgcggg tcatccgcaa
     2461 caacgccaac attggtttca gtaaaccggt caaccaaatg ctggctgcgg cgcgggggga
     2521 attctttctg ctgctcaacc ccgacacgct gttgatcgag gacctattca cgccaatgct
     2581 ggattggatg cgcgcgaatg agggcgtggg gattgccatc ccaaaggtgt tgaacgcgga
     2641 cgggagcttt cagaagcaga gccggagggg cgaggccaga cccgcggagg tgttcgggta
     2701 ttttttatgg ctgggcaggc ttttcccgcg gaaccgcgcg ctgaacggct acctgcaaag
     2761 ttggcttccg gaggacgaaa tcgcggaggt gaaggctgtt tcaggttcgt gcatgtttat
     2821 ccggcgagcc gcctgggagc aaatcgggga gttcgacgag cgcttttttg cctaccagga
     2881 ggacagcgat tactgcttgc gcgcccggaa ggcgggttgg aaggtgatgt atgtaccgat
     2941 tgcgcgcatc gtccattacg gcggggaggg cggcagcaag gtgcggccgg ctaactctat
     3001 cctggcgtgg caccgctctt actttctgta ctaccgcaag cacttcgcgc gggagtattt
     3061 ctttctgttc aactggtttt tctacctgat gatgggcgcc aagctggttt tggcgctgct
     3121 caaaaacctg ttccgtcagc tgataaatta agcggttgtc caaaaagaag gatttttcct
     3181 ttttggacag cccaaagtgg gagaacgtgc ctggtttatc ctcattttga gagcgcaaat
     3241 ccgccaacga ccataaaaac gggcacggag tgggaaatca aaaagggccg tccgactttt
     3301 gggacagccc ctttttttat gtggggggga gactaacggc tcaagcgcaa aatcgcggcg
     3361 gcgtctttgc cgctgatgtc ttgatgctcg ccgaaagtcc actgatggga ttccatgcgc
     3421 gcctgcacct tttgggcggc ttcctcgggg tcaatgccgt aggctgtgag cgtggtgggc
     3481 atgcccaggc tgtggaagaa agcagctgtg gcttcgatgg tctggtcgat gcggtcgtcg
     3541 tatttctggg tcttgatgcc gagcacgcgg tagccgtatt gcagcagctt ggcctgtttt
     3601 tgcttgcgtt tgtaccacag cagccagggc agcaccgccg ccagcgtctc ggcgtggtcc
     3661 aggccgtaga gcgcggtgag ctcatgcccg atattgtggg tggcccagtc ttccggaacg
     3721 ccgcggttga gggtgtggtt gagggcgttg gtggcggccc acatgaagtt ggcgcgagcg
     3781 ttgtagtcgg gcggcacgcg cagcgcgtcc ggggcgtgat ccctcaatgc catcaggatg
     3841 gcttccgcct gccggtcttg caccagggcg ccgaccggat aggtgaggta ttgctcgaag
     3901 acgtgcacga aggcgtccac cagcccgttg cggatctgct tttggggcag ggaataggtc
     3961 gtttcggggt ccaggatgga aaagcgcggg aagaccaggt cgctgccgaa ggagagtttc
     4021 tcctggatgc tgcggcggga aatcacggcg ttgctgttca tttcgctgcc ggtggcgggc
     4081 aaggtgagga cggttccgaa cggcagggct tctttggcct gggagagacc gccgcctgtc
     4141 agaatatccc aggggtcttt gccggggtag agggctgcca gagaggcgaa tttgacgccg
     4201 tcgatgaccg aaccgccgcc gaccgccagc agaaagttga cgtgcttttt ctggattttc
     4261 ctgaccgcgc gcatgagcgt gtcgtagtcc gggttggctt cgatgccgcc gaattccagc
     4321 acttcgcgct ttttgagggc tgccatcacc tgctcgtaga cgccgttctt tttgatggag
     4381 ccgccgccgt aaagcaccat cactttggct ttgcggggga tgagcttgtt gattttggca
     4441 atcgtgccgc tgccaaagaa gatttgggtg gggttatagt atcgaaagtt ttcaatcatg
     4501 gtgagtcctc ctggatgagc ctgggggtga tgatggcaca gttgaatcgt gggccctgtt
     4561 gtggattata tcccagaccg agcttccagg gggctgcgcg tggaggctgc gcgtggaggc
     4621 gcgtccggct gatttgcagg agaagcatcc ggagggaaag ggcaggatct ccttcgctgg
     4681 cgctccggat gacaaaacgg ggcgctcagg atgacaagcc gcataagctg ggtgcagatg
     4741 cgcgggggcg gaacctaacg actacttcac atagctgctg gccaaagctt gtcggg
//
LOCUS       DAOQAD010000130         4629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_23810_length_4629_cov_4.888500, whole genome shotgun sequence.
ACCESSION   DAOQAD010000130 DAOQAD010000000
VERSION     DAOQAD010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23810_length_4629_cov_4.888500"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..420)
                     /locus_tag="PKL60_08175"
     CDS             complement(58..420)
                     /locus_tag="PKL60_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96148.1"
                     /translation="MDPGHIADGLKTILRVLDALPPEHLPDLSRYQRGSEPILGPEPG
                     LLGREALINALDCLHAAVQTRLAEMEEAAFDEEMIIFGSQAHRRGWWALFFLFHHSYH
                     VGQLEIQRNLAGRTEKLI"
     gene            complement(336..545)
                     /locus_tag="PKL60_08180"
     CDS             complement(336..545)
                     /locus_tag="PKL60_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96149.1"
                     /translation="MITISEYAHILEREQDIIHLQTEGLGDTDLLVQPANGGNCMEWT
                     LGISPTDSRPSCAFWTRFRLSIYPT"
     gene            complement(591..1817)
                     /locus_tag="PKL60_08185"
     CDS             complement(591..1817)
                     /locus_tag="PKL60_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW96150.1"
                     /translation="MLSETNQLRRNIRIFSLYFLIFFAGMGFIQPFLSLHFRSIGFSG
                     VQVGALGTFSSLGLILTATQYGVFFDRSSRKRLILALSLFLLTLTLFFLPYLRGFGIV
                     LALYVLHRIVTSSSITATENLSYQVSSDSAANGKASFGKLRMWGSVGFGIAAVIGGWL
                     FGAKGILVNDQLFLAFMLVSLGFILFVMPTDFFRDGKTDQKQGAAAGLKGIVRLIFAD
                     RYLLLTVVALALTDPLSDGVRSFEPIFMKDLGLTESIIGAAVSLSCFTEVPIMLLGDR
                     WIQKLEVHQLVLFILIFDLTRRLLVWFFPFGWLVFVLNVLTAVSFTLRLITTIHLINL
                     RVPRQYTTAAVTFVNITLNGTSHMLSNALSGVVYDNFGGRPIYLVSACFCVISLVFAL
                     AARRAKAQESLPPALE"
     gene            2398..3534
                     /gene="dnaN"
                     /locus_tag="PKL60_08190"
     CDS             2398..3534
                     /gene="dnaN"
                     /locus_tag="PKL60_08190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNW96151.1"
                     /translation="MKAIVKQQQLAQAVGIVARAVTSRSPLPVLANILIQTDNGRLKL
                     SATNLELGISAWLGAKILDEGSLTVPARTFADLVGNLPSDDVTLTADLRTQSLNVHCG
                     TLNTDIKGISAEEFPPMSAADTGISLPLNITNFKEMIQKVVFAASHDESRPNLTGVHM
                     TVEGDYLVLAATDGYRISIASAILAQKPAQKLDALIPARALSELSRVATDGEEILQMN
                     FPVGRGQVVFHLKDVELISQLIEGNFPNYTAIIPPSFKTRTVLTTADLLKACRQTEII
                     AREGNFIARLDIQPEGSTEKTSQLEISAISEQTGSSEVIVDASVDGTPLLISFNVRFL
                     REVLEVIRTANVILETNAANTPAMIRPVGDDTFKHIIMPMNIQR"
     gene            4281..>4629
                     /locus_tag="PKL60_08195"
     CDS             4281..>4629
                     /locus_tag="PKL60_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96152.1"
                     /translation="MTLPQSGSDRYYSIYLALSQYPVLSGRIRELMRQELYQKGVTNP
                     TALNAEAVQNAVQSQEREGLRDPLTEEDSETWEYRKSAILNQLTDTYFAKFCGFEQLT
                     RLIQTALNERGVQA"
BASE COUNT         1087 a         1320 c         1296 g          926 t
ORIGIN      
        1 agccggaagc atcgctcatt gccgttatgc gcccccgata tgtagccgtt tcgtaattta
       61 gattagtttt tcagtcctgc cggcgaggtt gcgctgaatt tcgagctgcc cgacgtggta
      121 actgtgatgg aagaggaaga aaagcgccca ccagccgcgc cggtgcgcct gactcccaaa
      181 gatgatcatc tcctcatcaa acgcggcttc ttccatctcc gccaggcggg tttggacggc
      241 ggcatgcaag cagtccaggg catttatcaa cgcttcccgc cccaggaggc cgggttcggg
      301 tcccaggatg ggctcgcttc cgcgctgata gcggctcagg tcgggtaaat gctcaggcgg
      361 aagcgcgtcc agaacgcgca ggatggtctt gagtccgtcg gcgatatgcc cagggtccat
      421 tccatgcaat tgccgccgtt tgctggctgc accaacaggt cagtgtcgcc taatccttcg
      481 gtctgcagat gaataatgtc ctgttccctc tccaggatat gcgcgtattc gctaatggta
      541 atcatatttt tcctttcagt tggcaatctg gcaggcagtg tgctgccaaa tcattccagc
      601 gccggcggca ggctttcctg cgcctttgcc cgccgggcgg ccagcgcaaa gaccagggaa
      661 atcacacaga aacaggcgct gaccaggtaa atcggcctcc ccccgaagtt atcgtacacg
      721 acgccgctga gcgcgttgct gagcatgtgg gaggtgccgt tcagagtgat attaacaaag
      781 gtaaccgcgg cggtggtgta ctgccgggga acgcgcaggt ttatcaggtg gatggtcgta
      841 atcagtcgca gggtaaaact gaccgcggtc agaacgttca acacgaacac cagccagcca
      901 aaggggaaaa accacaccag cagccggcgg gtcaggtcaa agatgaggat gaacagcacc
      961 aattggtgga cttccagctt ctggatccag cggtcgccca ggagcatgat ggggacctcg
     1021 gtaaagcagc tcagcgaaac cgccgcgccg atgatgctct ccgtgagccc caggtccttc
     1081 atgaagatgg gctcaaaaga gcgcacaccg tcgctgagcg gatccgtcag cgcgagcgcg
     1141 accacagtga gcaaaagata gcgatccgcg aagatcagac ggacaattcc tttgagccct
     1201 gcggccgcac cttgtttctg gtcagttttt ccatcgcgga agaaatccgt gggcatcaca
     1261 aaaaggatga atccaaggga gacgagcatg aacgccagga agagctgatc gttcaccagg
     1321 atgcctttcg cgccgaagag ccagccgcca atcaccgcgg caatgccgaa accgaccgag
     1381 ccccacatgc gcaatttgcc aaaggatgcc tttccgttag cggcgctgtc ggaggagacc
     1441 tggtaagaga gattttcggt ggcagtgatg ctggagctgg tcacgatgcg gtgcagcaca
     1501 tagagcgcca gtacgatgcc aaagccgcgc aggtagggca ggaagaagag tgtcagggtg
     1561 agcaggaaca gggaaagcgc cagaatcaag cgtttgcggg agctgcggtc aaaaaatacg
     1621 ccgtactgtg tggcggtcag gatgaggccc agcgaactga acgtccccag ggcgcccacc
     1681 tgcacgccgc tgaagccaat cgagcggaaa tgcaggctga ggaagggctg gataaaaccc
     1741 atgccggcga agaagatgag gaaatataag ctgaagatgc gaatattcct gcgaagctgg
     1801 ttggtttcgg aaagcatgct gctccaataa tgacaggacg gtgcaaaacc cggtgttctg
     1861 tcggaaaatt atatcatcct ggcatgggaa agggctgaaa acacggctga atgggggctg
     1921 ggtgcaatcg ctaataaaag cttcttctgg gggctgcctg catgccggag accaggctgg
     1981 cgggtgtaga aaccttatat agcttcccct cggggctgcc ggcatgccgg aggccaggct
     2041 ggcaggcgcg gcgagcctgt agaggagtat tctcctcgcg cggattccca ggcaatccac
     2101 ctgcctagcc attcctgcgc agggacaggc agaagcttaa catttatccg aaaaaactcc
     2161 caagggatgc ttcgctcata catctgacac aagccgaagc gtgcagagct ggtgtaaagt
     2221 gcagtgccag gagttttccg ccggcttgaa atgcgcaagc cgcacctgaa aaacatcctt
     2281 atctaggcgg cgccccgcgg agagaaaacg ctaacctacg cgttcatctg gcaatctgcc
     2341 cggcttcacg gtataattta accggttaat gaccatttgc atccgcggga ggaactcatg
     2401 aaagctatcg taaaacagca gcagttggct caagccgtcg gcatcgttgc cagagccgtc
     2461 acgtcccgca gcccgctgcc ggtgctcgcc aatatcctca tccaaaccga caacggccgg
     2521 ctgaagctct ccgccaccaa cctggaactg ggcatcagcg cctggctcgg cgcgaagatc
     2581 ctggacgaag gcagcctcac cgttcccgcc cgcacttttg ccgacctggt gggcaattta
     2641 cccagcgacg acgtcactct cacagccgac ctgcgcacgc agtcgctcaa cgtgcactgc
     2701 ggcacgctga acacggacat caagggtatc agcgcggagg aattcccgcc tatgtccgcc
     2761 gcggatacgg gcatcagcct gccgttgaac atcacgaact tcaaagagat gatccagaag
     2821 gtggtttttg ccgcctcgca tgacgaatcc cgcccgaacc tgaccggcgt gcacatgacc
     2881 gttgaggggg attacctggt gttggccgcc accgacggct accgcatttc cattgccagc
     2941 gcaatcctgg cgcagaaacc tgcccaaaag ctggacgcgc tcattccggc ccgggcgctt
     3001 tccgagcttt cgcgcgtggc aacagacggc gaggaaattc tgcagatgaa cttcccggtc
     3061 gggcgcggtc aggtggtctt ccacctcaag gacgtggaac ttatctcgca gctgattgag
     3121 ggcaacttcc ccaactacac cgcgattatc ccgccctcgt tcaaaacccg caccgtgctg
     3181 accacagccg acctgctgaa ggcctgccgg cagactgaaa tcatcgcgcg cgaaggcaat
     3241 ttcattgccc ggctggatat ccaacccgaa ggcagcaccg aaaagacctc ccaactcgaa
     3301 atttccgcga tttccgaaca aaccggctcc tccgaagtga tcgtggatgc ctccgtggac
     3361 ggcactccgc tcctgatatc cttcaatgtg cgcttcctgc gcgaagttct ggaagtcatc
     3421 cggactgcca atgtcattct ggagaccaac gctgccaata cccccgcgat gattcgtccg
     3481 gtaggggacg ataccttcaa acacatcatc atgccgatga acattcagcg ctaaaaggct
     3541 tgagaggaaa cctaccggac gaaaaaatcg tccggttttt tatgttccgg gcagcttttg
     3601 ggcagggagc gcggaaagct gcgtgaaccg ccggactggc aaacgttttc ctgtgacagg
     3661 tggttattcc aggaaagaaa attgcgcata gattaatgaa gcacagtaag actgaaaaga
     3721 aatggcttca tgcgggcaat tttgaaatgt cctgattcgg ggataaagtc atcaggtcgt
     3781 gtttttcctc gaaactaatc ccctgatttc ttcttctcca ggttgtttcc cgcttgccct
     3841 gagcctgacc cagcggtgag caactctgtt ttgcctcggt ctcggcgctg cggaagggaa
     3901 attcgtttta ttgacgtccg cgccctcact caaagatcaa gtcctccgcc ccggcgggag
     3961 ttcatcagga tcccaggtta aattgagtcc gcaagggaag gggtgtcagg agggaaagct
     4021 gctattcccc tgggtgtcag ggtatgatca ttcctttgta aaaaacttgg aggaatcgaa
     4081 ggaaaatttc ataactccta atctttatcc ggatatttct attgtgctgt ttcaggacat
     4141 gattcctttg ctcttccaca gttaaatata gcgtgccgtt gctttaataa tattgatgtc
     4201 cttctatatt attgccagcg gaccgctgta atttcttcca ggcgtgataa aatcaggcat
     4261 atccctccgc gaggcacccc atgaccttac cgcaaagcgg cagcgaccgc tactacagca
     4321 tttacctggc gctctcccaa tacccggtcc tgtccgggcg catccgcgag ctgatgcggc
     4381 aggagctgta ccaaaaaggc gtaacaaacc cgaccgcctt gaacgcggaa gcggttcaga
     4441 acgcagtcca atcccaggag cgcgaaggcc tgcgcgaccc gctcacggaa gaggactccg
     4501 aaacctggga ataccgcaag tccgcgattc tgaaccaact aacggacacc tacttcgcca
     4561 agttttgcgg gttcgagcag ctcacgaggc tcatccagac tgccctgaac gagcgcgggg
     4621 tccaggcgc
//
LOCUS       DAOQAD010000131         4521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_24674_length_4521_cov_6.328482, whole genome shotgun sequence.
ACCESSION   DAOQAD010000131 DAOQAD010000000
VERSION     DAOQAD010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24674_length_4521_cov_6.328482"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /gene="lepB"
                     /locus_tag="PKL60_08200"
     CDS             <1..570
                     /gene="lepB"
                     /locus_tag="PKL60_08200"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNW96153.1"
                     /translation="PELSSPDPVPNKTHKTNFWIDTLETIVLALVLFLAINAVSSRVR
                     VENISMKPTLQPGYLLVVNKLAYKLGEPKHGDIVVFDFKGAGQEDYIKRIIGLPGDQV
                     VVENGVVSVNGHPLTEPYISEMPRYSGSWSVPEDSYFVLGDNRNNSSDSHQWGYVNEA
                     DLVGKAVFIYYPFNAIRSLVITDLVQAAN"
     gene            650..940
                     /locus_tag="PKL60_08205"
     CDS             650..940
                     /locus_tag="PKL60_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HNW96154.1"
                     /translation="MKPTLPADPETCPRCMVGHIQRKRIPYASVIQGQLLTIPHFPAW
                     VCDVCHAYMYDPAAMSRLEASLSAGQLPKKASRTRQAAPTSHPSADKPLESL"
     gene            1103..2179
                     /locus_tag="PKL60_08210"
     CDS             1103..2179
                     /locus_tag="PKL60_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HNW96155.1"
                     /translation="MSAVLLSRIIGAVVFGIIGFFSGTPIHNYLISFWASYPFTNGIT
                     VAICIILFMLIGYIISPWISVKPVLAISKSLGKSSPQAFFSGLIGLIISLIISALLAY
                     PVSLLPEPFGQFMPLAMVVVLGYLGMTLFYNRNQEFLSIFRSFSNKMTTKGSRTQVDG
                     EHRVLVDTSAIIDGRIADIAKTGFLTGTLIVPRFVLNELQYVSDSADSLRRQRGRRGM
                     DVLTELQHDEKSTVVISDIDVEGVREVDDRLVVLARQMRCPILTNDYNLNRIAELQGV
                     KVLNINDLANAVKVILLPGESFDVRVIQEGKEFNQGVGYMDDGTMVVVENGQRYLNKE
                     INVTVTKILQTSAGRMIFAKREVE"
     gene            2196..3596
                     /gene="cysS"
                     /locus_tag="PKL60_08215"
     CDS             2196..3596
                     /gene="cysS"
                     /locus_tag="PKL60_08215"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNW96156.1"
                     /translation="MTLRIYNTLTREKEDFRTLEPGKVRMYVCGPTVYDRAHIGHAMS
                     ALVFDVVRRYLEYSGYEMRFVMNFTDVDDKIIDRARQSGADPFQLAQGYIEDYKRNLE
                     ALNVKPATVNPRATQEMAHILEMIQALVEKGCAYAVDGDVYYRVESDPDYGKLSGRRL
                     DSMNAGARVQVDERKEHPMDFALWKAAKEGEPAWDSPWGKGRPGWHIECSAMNMHHLG
                     EQIDIHGGGNDLIFPHHENEIAQTEALTHKPFATYWMHNGMLTLKSEKMSKSLGNMVL
                     IDEFLAEHPGDVMRLLVLNGSYRSPLTYNDEVLAQTEQAYKRLLSAKKAADPDSPGLD
                     PDTAAALEADAEKTRKQFREAMDDDFNSSAALAALFELVRAINQARADSASQAQLAPA
                     VRVFEELTGVLGLQLNEEAGASTKADAFIDLLVALRTELRAQKLWALADKVRVELKAL
                     GVVLEDGKNGTSWTWE"
     gene            3599..4336
                     /gene="rlmB"
                     /locus_tag="PKL60_08220"
     CDS             3599..4336
                     /gene="rlmB"
                     /locus_tag="PKL60_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNW96157.1"
                     /translation="MREWITGRNPVYEVLRAKRRHVFRLVLARGIKMEGQLPEILRLA
                     KLRGLAAEEAPRDKLDALSSHHQGVALEASAYPYAALEDLIQHARNQSEPPFFLLLDL
                     IQDPQNLGTLLRTAEAVGVHGVIMPSAQSAQITPTVVHASSGAAEHLLVAQYNLTQAI
                     GKLKTLDIWVYGLDEDPRSKTPSQLRMDGGLALVVGNEGAGLRDLVRRSCDELLRLPM
                     RGKIESLNAAAAGSIALYLARQARDEA"
BASE COUNT         1024 a         1314 c         1266 g          917 t
ORIGIN      
        1 cctgaattaa gcagcccgga ccccgtccca aataaaaccc acaagacaaa cttttggatc
       61 gatacgctcg aaacaattgt ccttgccctg gtgctctttc tggcgatcaa cgcggtcagt
      121 tcgcgcgtgc gggttgaaaa tatcagcatg aagcccaccc tgcagcccgg ctacttattg
      181 gtggtcaaca agctggccta taaacttggc gagcccaaac acggcgatat cgtcgttttt
      241 gatttcaaag gcgccggtca ggaagattac atcaaaagaa tcatcgggct gcccggcgac
      301 caggttgtgg tcgagaacgg cgtcgtaagc gtgaacggac acccgctgac cgaaccatac
      361 atatccgaga tgccgcgcta ttccggcagt tggagcgttc ccgaggattc ttacttcgtc
      421 ctgggcgata accgcaacaa ctcctccgat tcccaccaat ggggatacgt caacgaggcg
      481 gacctggtcg ggaaagccgt attcatctac tatcccttca atgccatccg ttccctggtc
      541 atcacggacc tggttcaggc ggcaaattaa acccgggctc cacacaacca gtatcttttt
      601 atcaatacat tgacaaacgc agcatccaaa taggcgtata attaccccga tgaaaccaac
      661 gctacctgcc gaccctgaaa cctgcccccg ctgcatggtt ggacacatcc aacgcaagcg
      721 gattccttac gcgtctgtca tccaaggcca gctgctgacc attccgcact ttcccgcctg
      781 ggtctgcgac gtttgccacg cgtatatgta tgacccggcc gccatgagcc gcctggaggc
      841 ttccctcagc gcgggccaac tgcccaagaa agcttcccgg acgcgccagg ccgcgcccac
      901 ttctcaccct tcggcggata aacctctgga aagtctctaa accccaaaag cgcagggggt
      961 accgcggtat ttaagcaaac ctctctatga gttgtgtaag aaaacgccgg ttttgttgac
     1021 ttagcgcact gacagtgcta tcatcttgaa acaggactca tacttttttt attcagtacc
     1081 ttaataaagg agggtttata ctatgagcgc ggtcttatta tctcggatta ttggcgcagt
     1141 tgtttttgga attattggct ttttttccgg cacaccgatt cataattacc ttatctcttt
     1201 ctgggcgtct tatccgttca ccaacggaat cacggtcgcg atttgcatta ttctcttcat
     1261 gctgattggc tacattatca gcccgtggat tagcgttaag cccgtcctgg ctatcagcaa
     1321 gagtcttgga aaatcatccc cacaggcatt tttcagcggg ttgattggtt taattatcag
     1381 cctgattatt tccgcgctgc tcgcctaccc ggtttccctt ctacccgaac cattcgggca
     1441 gtttatgcct ttggcgatgg tggtggtgct gggctacctt gggatgaccc tcttctacaa
     1501 ccggaatcag gaattcctga gcatcttccg gtctttctcc aacaaaatga ccaccaaggg
     1561 ctcgcgcacc caggtggatg gcgaacaccg cgtgctggtg gataccagcg cgattatcga
     1621 tggacgcatc gccgacattg ccaaaaccgg ttttctcaca ggcactttga ttgtgccgcg
     1681 ctttgtgctc aacgaactcc agtacgtctc cgactccgcg gattctctgc gccggcagcg
     1741 cgggcggcgc gggatggatg tgctcaccga gctgcagcac gacgaaaaat ccaccgtggt
     1801 catcagcgac attgacgtcg aaggcgtgcg agaagtggac gaccgcttgg tcgtgttggc
     1861 gcgccagatg cgctgtccca tcctgacaaa cgactacaac ctgaaccgca tagccgaact
     1921 gcagggcgtg aaggtgctga atatcaacga ccttgccaac gcggtcaaag tcatcctcct
     1981 gcccggcgag agctttgacg tgcgcgttat ccaggaaggc aaggaattca accagggcgt
     2041 cggctacatg gacgacggca cgatggtggt ggttgaaaac ggtcagcgtt acctcaacaa
     2101 ggaaatcaac gtgacggtca ccaaaatcct gcaaacatcc gcgggacgca tgattttcgc
     2161 gaaacgcgaa gtggaataac tgcgcgagga aggccatgac ccttcgaatt tacaatacgc
     2221 ttacccgcga aaaagaagat ttccgcacgc tggaacccgg caaagtgcgc atgtacgtgt
     2281 gcggacctac ggtctacgac cgcgcgcaca tcggacacgc catgtccgcg ctggtgttcg
     2341 atgtcgtccg ccgctacctg gaatattccg gctatgaaat gcgctttgtg atgaacttta
     2401 ccgacgtgga cgataaaatc atcgaccgtg ccaggcagtc cggcgcagat cccttccagc
     2461 tcgcgcaagg ctacatcgag gactacaagc gcaacctgga ggctctgaac gtcaaacctg
     2521 caaccgtcaa cccgcgcgcg acgcaggaaa tggcgcacat cctggaaatg attcaggcgc
     2581 tggttgaaaa aggctgcgct tacgcggtgg acggtgatgt gtattaccgt gtggagtccg
     2641 acccggacta cggcaagctc tccgggcgcc gcctggatag catgaacgcc ggcgcgcgcg
     2701 tccaggtgga cgagcgcaag gagcacccga tggactttgc cctgtggaag gccgccaagg
     2761 aaggcgagcc cgcctgggac agcccctggg gcaaaggacg cccgggctgg catatcgaat
     2821 gctcggccat gaatatgcat cacctgggag agcaaatcga catccacggc ggcggaaatg
     2881 acctgatttt cccccaccac gagaatgaaa tcgcccagac cgaagcgctg acccataagc
     2941 cattcgccac atattggatg cacaacggca tgctcaccct caagagcgag aaaatgtcca
     3001 aatcattggg caacatggtc ctgattgatg aatttctggc ggaacacccc ggcgacgtga
     3061 tgcgcctgtt ggtgctaaac ggctcctacc gcagcccgct cacctacaat gatgaagtgc
     3121 tcgcgcagac cgaacaggcg tacaaacgcc tgctcagcgc gaagaaagcc gccgaccctg
     3181 attctcccgg gctcgacccg gacacagcag ccgcgctgga agccgatgcg gaaaagacgc
     3241 ggaaacagtt cagggaagcc atggacgacg acttcaacag ttccgcagcg ctggccgccc
     3301 tgttcgaact cgtccgggct attaaccagg cgcgcgcgga tagcgcgagc caggcgcagt
     3361 tagctcctgc tgtacgcgtc tttgaggaac tgaccggcgt tttggggctg cagctgaacg
     3421 aagaagccgg cgcgagcacc aaagcggatg ccttcatcga cttgctggta gcgctgcgca
     3481 cggagctgcg cgcgcaaaaa ttatgggcgc tggccgataa ggtgcgcgtg gaactgaaag
     3541 ctctcggtgt ggtgctggaa gacggcaaaa acggcacgag ctggacctgg gagtaggctt
     3601 gcgcgagtgg attaccggcc gcaacccggt ctacgaagtg ttacgcgcaa agcgcaggca
     3661 tgttttccgc cttgtgctcg cccgcggaat aaagatggaa ggccagcttc cggagatttt
     3721 gcggctggcc aaactgcgcg gcctggcggc ggaggaagcc ccccgcgata aattggacgc
     3781 gctttccagc catcaccagg gcgtggcatt ggaagccagc gcttacccct acgccgccct
     3841 cgaggatttg atacagcacg ccaggaacca atcagagccg ccgttcttcc tcctgctcga
     3901 ccttattcag gacccgcaga acctgggcac gctgcttcgc acggcggaag cggtgggtgt
     3961 gcacggggta attatgccct cggcacagtc cgcgcagatt actccgaccg ttgtgcatgc
     4021 cagttcgggc gccgcggagc atttgttggt ggcgcaatac aacctgacac aggcgattgg
     4081 aaagttgaaa acgctggata tctgggtgta cggattggac gaagacccgc gctccaagac
     4141 tccttcgcag ctgcgcatgg acggcggatt ggcgctggtg gtcgggaatg agggcgcggg
     4201 gctgcgcgat ttagtgcgga ggagctgtga tgagctgctg cgcctgccta tgcgcgggaa
     4261 gattgagtcc ctcaacgccg cagcggctgg ctcgattgcg ctttaccttg cgaggcaggc
     4321 aagggatgag gcttgagcgg attcgcgaga cgcacgggca ctttatcgtg tttatcatcc
     4381 aaagcgccag ccagaaatat tctcttcaac atgttgctcg ggtaactgcg gcgcaggagc
     4441 gccccccaac gacggctcca caaacccgcc ggccaaagct tgtaggttgg gtgcagcgac
     4501 gcaggagcga aacccaacga c
//
LOCUS       DAOQAD010000132         4321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_26423_length_4321_cov_4.906001, whole genome shotgun sequence.
ACCESSION   DAOQAD010000132 DAOQAD010000000
VERSION     DAOQAD010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26423_length_4321_cov_4.906001"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PKL60_08225"
     CDS             <1..990
                     /locus_tag="PKL60_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNW96158.1"
                     /translation="PVMVDGKLSMILQVARPLTERDRSMEGLRNTLLSATLFTMIAAV
                     AIGWFFSGYVLKPIQKIAQTARQIGDEQDFSRRVDYQGPQDEVGQLAVTFNTMLSQLE
                     DAYEAKTRALEMQREFVADVSHELRTPLTTLRGNLSLLSHQPAVPKEEQADILKDMVD
                     ETERMIRLVSALLEQARADSVHGAAREKVDISALLEEAVRHVRQMDENRQISQSAPAG
                     LSLLTWRDGLKQVLLILLDNALKYTPGSVDVEAEAREDFVEIRVRDHGAGIPEEKLTH
                     VFDRFYRGGSASGTGFGLGLSIAKSLVDAMGGQLAIESRVGAGSTAIISLPNK"
     assembly_gap    1272..1371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1539..2879
                     /locus_tag="PKL60_08230"
     CDS             1539..2879
                     /locus_tag="PKL60_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HNW96159.1"
                     /translation="MSQITHVVKRNGTLVPFTPERITNAIYRAAVAVGGRDRKTSEEL
                     AAQVVALLEQNTPAGEPPTIEQIQDLVEKVLIENGHARVAKAYILYRDERARQREKRK
                     EPDQQPSSNIPWAKLWSVLDWACSHEVHTVAKLNAHIRAGSLHQIVTAAEAAYHEDVE
                     NAANLVLSRKDEVRMVFIAGPSSSGKTTTTIKLGEQLARENLRLVTLNVDHYFHDLEL
                     HPKDEFGDYDFETPQALDMDLINQHLSALFDGQEVRIPYYDFKTGKSIKDHTPMRLAK
                     NEIILIDSLHGLYPEMTASIPAEMKFKLYIEPLLQLKDSAGHYVRWTDIRLIRRMLRD
                     ASHRAYDPTRTLLHWHYVRSSELRHIIPYINSVDTIVNSAMPYELPLYKAKLGADFAR
                     WTDAYRDDPLHQDAYERAARVNALFAELDAVADDSIVPENSVIREFIGGGIYKY"
     gene            complement(2948..3616)
                     /locus_tag="PKL60_08235"
     CDS             complement(2948..3616)
                     /locus_tag="PKL60_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96160.1"
                     /translation="MKKPSKTLALCLFVFLLVGCTASAAPSATPQPSAIPTLAPSPTY
                     TTAPTESPTPEPSPTFTAAPTATNTLIAFPGFSHLFELNKAWIADNQTHFYFLNAQVN
                     MKLYGTIAGEYPFTCSPDPKYPNHMECVSDFTDWKKSWVEFVFFADEAHTQEVDRRTF
                     FTGLSDEARGIPEGCEAEFRIYDGKCYVAYTCYDALGNYLYSYNNYPPGGTFQGFTGP
                     CPGW"
     gene            complement(3624..>4321)
                     /locus_tag="PKL60_08240"
     CDS             complement(3624..>4321)
                     /locus_tag="PKL60_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96161.1"
                     /translation="EDPMKNTLSRLILFALMCTLLAACAGAAPQPTATPDATNTPTIP
                     PPTDTPAPTLTPTQTEAPTETATPTRTKVPFAGFMQLFSLYRAWSQDGKTYFYFLQSN
                     FPGTMYARADSYDLVCNPDPKYPLHMQCESSVEIFGQDEMTFTFFTDEDRQDLVHTQK
                     FFTGLVNDIVYYHNTDCPDRGKNIHCESEYRLYDGRCYYAHTCYDACGLYYSRDNLPD
                     VWNEFQGYTTPCN"
BASE COUNT          917 a         1157 c         1313 g          834 t
ORIGIN      
        1 ccggtcatgg tggatggaaa actctccatg attttacagg tcgcccgacc tctgaccgaa
       61 cgagaccgca gtatggaagg gctgcgcaac acgctccttt ctgccaccct gttcaccatg
      121 attgcggcgg tcgcgatcgg atggttcttc tccgggtatg ttcttaagcc gattcagaaa
      181 atcgcgcaaa ccgcccgcca gattggtgac gaacaggatt tttcccgccg ggtggattat
      241 caaggccccc aggatgaagt cgggcagctg gctgtgacgt tcaataccat gctctctcag
      301 ttggaagacg cctacgaagc caaaacccgc gcgctggaga tgcagaggga gtttgtggcg
      361 gacgtttcgc acgagctgcg cacaccgctg accaccttgc gcgggaacct gagcctgctt
      421 tcgcatcagc ccgcggttcc gaaggaggag caggcggaca ttctgaagga tatggtggac
      481 gaaaccgaac ggatgatccg cctggtcagc gcgctgttgg agcaagcccg cgcggattcc
      541 gtccacggcg ctgcccggga gaaggtggac atttccgctt tgttggaaga agcggtgcgc
      601 catgtgcgtc agatggatga aaaccggcag atctcgcaaa gcgcgccggc ggggctgagc
      661 ttgctcacct ggcgggacgg tttgaaacag gtgctgctga ttttgttgga taatgcgctg
      721 aagtacaccc cgggaagtgt cgatgtggag gccgaggcgc gggaagactt tgtagaaatc
      781 cgggtgcgcg accacggcgc tggcattcct gaggagaagc tgacgcatgt cttcgaccgc
      841 ttttatcggg gagggagcgc cagcggaacg ggctttgggt tggggctctc gattgcaaag
      901 agcctggtgg acgccatggg cgggcagttg gcgattgaga gccgcgtggg cgcgggctcc
      961 acagcgatta tcagcctgcc gaacaaatag aagagcgccc gcagggcgag ggagctctga
     1021 cccctcccca acatgcggag cgccgcagag tggatgagag aaagaatatc gtcccgcctt
     1081 cggatttcaa gcccgcgcct ttgtgtctat cagacgcaga ggcgaagcat ccttaaagga
     1141 ggatttcaga caagccggaa agtttggcag ggatacttcg ccgtacttta gatgatcgat
     1201 gggtggattt cacactccaa ggaaggcaca tcacaaggca gatatccgca tcagtctcgc
     1261 tgcgtccgct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naggaaggca
     1381 catcacaagg cagatatccg catcagtctc gctgcgtccg ctggtatccc ctcagaggca
     1441 tgacacgaca taccatcacg atgtcgatcc gcaaacgata gaacccccac gcgtgctata
     1501 atcccagcat cagctcaatt ctcaccgcgg aggagcgcat gagtcaaatc acccacgtcg
     1561 taaaacgcaa cggtaccctc gttcctttca cgcccgaacg catcaccaat gccatctacc
     1621 gcgcggctgt cgccgtcggc ggcagggacc ggaaaacctc agaagaattg gcagcccagg
     1681 tggtggcgct gctggagcag aacacccccg ctggcgagcc ccccacgatt gaacaaatcc
     1741 aggacctggt cgaaaaagtc ctgattgaga acgggcacgc ccgcgtcgcg aaagcctata
     1801 tcctttaccg ggatgagcgc gcgcgccagc gggagaagcg caaggagccc gaccagcagc
     1861 cctccagcaa catcccctgg gcaaaactgt ggagtgttct ggactgggcc tgcagccacg
     1921 aagtgcacac ggtcgcgaaa ttgaacgcac atattcgcgc cggcagcctg caccagattg
     1981 tgaccgccgc ggaagccgcc tatcacgagg acgtggaaaa tgccgccaac ctggtactca
     2041 gccgcaagga tgaggtgcgc atggtgttca tcgccgggcc ttcctccagc ggaaagacca
     2101 ccactaccat caagctgggc gaacaactgg cgcgcgaaaa cctgcgcctg gttaccttga
     2161 acgtggatca ttacttccac gacctggagc tgcaccccaa agacgaattt ggggattacg
     2221 atttcgaaac cccgcaagcg ctggacatgg acctcatcaa ccagcacctg agcgcgcttt
     2281 ttgacgggca ggaagtgcgc atcccatatt acgacttcaa gaccggcaaa agcatcaaag
     2341 accacacccc catgcggctg gcaaagaatg aaatcatcct gattgacagc ctgcacggct
     2401 tataccccga gatgacggcg tccatcccgg cggaaatgaa attcaagctc tatattgagc
     2461 cgctgctgca gctgaaggac agcgcggggc actacgtacg ctggaccgat atccgcctga
     2521 tccgccgcat gctgcgcgat gcctcgcacc gggcgtacga cccaacccgc accctgctgc
     2581 actggcacta cgtgcgcagc agcgaactgc gccatatcat tccttatatt aactcggtgg
     2641 ataccatcgt caacagcgcc atgccctacg aacttccgct ttataaagcc aagctcggcg
     2701 cggattttgc gcgctggacc gacgcgtacc gcgatgaccc gctccatcag gacgcgtatg
     2761 agcgcgcggc gcgcgtgaac gcgctctttg ctgagcttga cgcggtggcc gatgacagca
     2821 tcgtgccgga aaactcggtc atccgcgagt ttatcggcgg cgggatttac aaatactgag
     2881 ccgcagccgg ttctccgcat cacaaaacag cgcccgcagt tgcgggcgct gtgtgcatct
     2941 taggcgtcta ccaacccggg catgggccgg tgaagccctg aaaagttccg ccggggggat
     3001 agttattata ggaatagaga tagttgccga gcgcgtcata acaggtgtag gcaacatagc
     3061 acttgccgtc gtaaatccgg aattcggctt cgcagccctc agggatgccg cgggcttcat
     3121 cgctgagacc ggtgaagaag gtgcggcggt ccacttcctg cgtgtgggct tcatccgcaa
     3181 aaaacacgaa ctcaacccag gattttttcc agtccgtaaa atcggaaacg cattccatgt
     3241 ggttcgggta cttcgggtcg gggctgcagg tgaatggata ctcgccggcg atggtgccat
     3301 acagtttcat gttcacctgg gcgttcagaa agtagaaatg cgtttgatta tccgcaatcc
     3361 aggctttgtt gagttcaaaa aggtgtgaga aaccggggaa cgcaatcagc gtgttggtgg
     3421 ctgtgggcgc cgcggtgaag gtcgggctgg gttcaggcgt gggggattca gtgggcgcag
     3481 tggtataggt tgggctgggg gcgagcgtgg ggatagcgct gggctgcggt gtggcgcttg
     3541 gagcggcgct ggccgtgcat ccaaccagca aaaacacgaa caggcacagg gcaagggttt
     3601 tacttggttt tttcatcgtt cactcagttg cagggggtgg tatagccctg gaattcattc
     3661 cagacatctg ggaggttatc ccgtgagtag tacagaccgc aggcgtcgta acaggtgtgc
     3721 gcgtagtagc agcgcccgtc gtagaggcgg tattcggatt cgcagtgaat gttctttccg
     3781 cggtccgggc agtcggtgtt gtggtaataa acgatgtcat tcaccaatcc ggtgaaaaac
     3841 ttttgcgtgt gcaccaggtc ctgccggtct tcatccgtga agaaagtgaa ggtcatttcg
     3901 tcctgaccga agatttccac gctgctttca cactgcatat gcaaagggta cttcgggtca
     3961 gggttgcaga ccaggtcgta gctgtcggcg cgggcgtaca tggtgcctgg aaaattggac
     4021 tgcaggaagt agaaataggt tttgccatcc tgggaccagg cccggtagag gctgaaaagc
     4081 tgcatgaagc ctgcaaaggg gaccttcgtg cgggtggggg tggcggtttc ggtcggcgcc
     4141 tctgtttggg tcggggtcag ggtgggtgcc ggggtatccg tgggcggggg aatggtcggt
     4201 gtgttggtcg cgtctggcgt ggcggtcggc tgcggtgcgg cgcctgcgca ggcagccaag
     4261 agcgtgcaca tcagcgcgaa tagtatgagc ctggaaaggg tgtttttcat cgggtcttcc
     4321 t
//
LOCUS       DAOQAD010000133         4282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_26807_length_4282_cov_5.409274, whole genome shotgun sequence.
ACCESSION   DAOQAD010000133 DAOQAD010000000
VERSION     DAOQAD010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26807_length_4282_cov_5.409274"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PKL60_08245"
     CDS             <1..993
                     /locus_tag="PKL60_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNW96162.1"
                     /translation="STLMPNISPAIAGQWGSALAMAGEMFGISVEVYMVNASYLAKPY
                     RRILMQTYGAQIFPSPSEHTKIGRGYLSKNPNHPGSLGIAISEAVEVAAESNGEKKYG
                     LGSVLNHVLLHQTVIGEEALKQMDLAGEYPDVVIGCVGGGSNFSGLSFPFLRANLRDG
                     RKTRFVAVEPTSTPSLTRGVYGFDYGDSFGIVPILKMYTLGSDFIPDPIHAGGLRYHG
                     MSPIVSALYHAGLLEAVAVGQRDVFAAAVQFARAEGILPAPESAHAILAAVNEAIDAR
                     DKNEKRVILFNLSGHGFLDLSAYDQYLHNGMISGSVPEAQLEGLAERLPKVKMP"
     gene            1347..2996
                     /locus_tag="PKL60_08250"
     CDS             1347..2996
                     /locus_tag="PKL60_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96163.1"
                     /translation="MEEPQAKHQLQPASTPTESKAKGIRILLLILAIAAFFLFAIPNA
                     KASDDLAMVQVFEPDESALFPVIMNMARPKGDFVIFVKQFIVYERYNKGFPFYGPSAL
                     ILLLLRGLGLGDNIPVNFLALRQLVSVLPMLCGLLLLVWLQDRFRTYRSVLLFLFLLI
                     TPAVVQNGFWWHPDGLTILLSALVLILLFKDQLSFGKYFNWAAAACGALTAAKTVGVF
                     FFLTIAALLVWGLVEKRVTPGKVVFLGFRFIAIMGAAFLVSNPFLFSEWGRAGYLSMM
                     RAQMSSLSSGYGVVYAKGISAAWPVMKENFGSALFLLASLGICVYGIAANKARLLNVL
                     ILTWFIPLSLYVTVFSHFKYQYWLPVALPLFSCWILVFPEKIRGFTPKDWRGAVRIGL
                     ALLFLSQFFSFALQSQSMFSARVHRAENNPYIQFSNTVAEKLAPVRGVPLRVYFDYRL
                     YVPDTPGWKLSTTYVPLSYAQIAAGDFDILLLAAQRVRDYLSPEAEGVDPALFAESQH
                     FYRDVEQRTIEGFTFLGGDNAGLVFIRQGLCAQYFPGLPCE"
     gene            3020..4237
                     /locus_tag="PKL60_08255"
     CDS             3020..4237
                     /locus_tag="PKL60_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96164.1"
                     /translation="MNTEPKKFDRVLTDFILRNLNLFFIVGISLLTLVIRNEFRGWQS
                     GDYLQHLLPWAKYLEAHGGFAGIASGPPNCNYPVTYQYILAFLTYLPGSYLAKIKVVS
                     VFFDFVSAAFVMLIVREIGGYAKKSFVPWLAYALALLIPTPILNSAAWGQSDSIYTAF
                     LVISLYFLIREKYPLAFIAFSLAFLFKLQAVFFFPVFLLLYLKNRKFSLLHFLIIPAV
                     IAVLSLPAIIIGKPLAEIFGVYIGQAQSYPKTTLNFPNMYFFLPDLHWQFAVPGILLT
                     IALILTLYYVLLKRERVVLAGGTLLEVSLLTVMICAYCLPSMHQRYLFTGDILAVIYL
                     FTRKERAYIPLVIWLISVNGYLWYLYGFGPLVEQRYLAMAYLWIMVLIARDLVFRPLN
                     PPQSLKEDNPPNL"
BASE COUNT          839 a         1301 c         1127 g         1015 t
ORIGIN      
        1 tccacgctga tgccgaacat ctctccggcc atcgccgggc agtggggttc cgcgctggcg
       61 atggccggag agatgttcgg catcagcgtg gaagtctata tggtgaacgc ctcttacctg
      121 gccaaaccct accgccgcat tctcatgcag acctacggcg cgcagatttt ccccagcccg
      181 tccgagcaca cgaaaattgg gcggggttac ctgtcaaaga accccaatca ccccggttcg
      241 ctcggcatcg ccatctccga ggccgtggaa gtggcagccg aatccaacgg cgagaaaaag
      301 tacgggcttg gctccgtgct gaaccacgtg ctgctgcacc agacggtcat tggtgaagaa
      361 gcgctgaagc agatggacct ggccggcgaa tatccggacg tggtcatcgg ctgcgtcggc
      421 ggcggctcca acttctccgg gctcagcttc ccgttcctgc gcgccaacct gcgggacggc
      481 cgcaagaccc gctttgtcgc agtcgagccc acctccaccc cttcgctgac gcgcggcgtg
      541 tacggctttg actacggcga cagcttcggg attgtgccga tcttgaaaat gtacacgctc
      601 ggcagcgact tcatccccga ccccattcac gccggcggct tgcgctacca cggcatgtcc
      661 ccgatcgtta gcgcgctgta tcacgccggc ctgctggaag cggtggcggt cggtcagcgc
      721 gacgtgttcg cggcggcggt ccagttcgcc cgcgcggaag gcatcctgcc cgcgccggag
      781 tccgcgcacg cgattctcgc ggctgtcaac gaggcaattg acgcgcgtga caagaacgaa
      841 aagcgcgtaa tcctgttcaa cctttccggt cacggcttcc tggacctctc ggcttatgac
      901 caatacctgc acaacgggat gatcagcggg agcgttccgg aagctcagtt ggaaggtctg
      961 gcggagcgcc tgcccaaggt gaagatgccg taactcaacc atgctccgaa aaagcgaggt
     1021 tgagcccaac cgtacttatt tgtttgatag ggcaggagcg cttcatgcgt caagcgttga
     1081 accgaaacat tagcgctctc cagactatgc agcccgataa tacgcaactc atcggccagg
     1141 cgggtccatt tccttttgca cggcccgccg gccgactcag cctttgacac gaataatcca
     1201 aagtcgaagc cagaaacttg ttgtgcgaaa aattggtgga aaacaatccg ctctaccaat
     1261 acacgtttat aatgcactgt aagccggagg aaaaatcctt tttccggaaa actacagagt
     1321 gaacccattg gtcaggagaa agttggatgg aggaaccaca agcaaagcac caattacaac
     1381 cagcctccac acccacagaa agcaaggcca aaggcattcg gattctcctc ctcatcctgg
     1441 caatcgcggc gttttttctc tttgccatcc caaacgcaaa agcttccgat gacctggcga
     1501 tggtccaggt ctttgagcca gatgagagcg ctctgtttcc ggttattatg aatatggcgc
     1561 ggcccaaagg tgactttgtc atattcgtaa aacagttcat cgtctatgag cgctacaaca
     1621 aaggcttccc gttttacggt ccctccgcgc ttatcctttt gctgctgcgc gggctgggct
     1681 tgggcgataa tatccctgta aactttctgg cactgcgtca gctagtcagc gtcctgccaa
     1741 tgctgtgcgg tctgctgctg ctggtctggc tgcaggaccg cttccggacc taccgttccg
     1801 tgctgctctt tctgttcctg ctcatcacgc cggctgtcgt ccaaaacggc ttttggtggc
     1861 acccggacgg ccttactatc ctgctttccg ccctggtcct catcctcctt ttcaaagacc
     1921 agctcagctt cgggaagtac ttcaattggg cagcagcggc ctgcggagcg ctgacagccg
     1981 ccaaaacggt gggcgtattc tttttcctca ccattgccgc gctgctcgtt tgggggctgg
     2041 tagaaaaacg cgtgactccg ggcaaagtag tttttctggg cttcaggttc atagccatca
     2101 tgggcgccgc gttcttggtc agcaacccct tcctgttctc tgaatggggg cgggcaggct
     2161 atctaagcat gatgcgcgcg caaatgtcct ccttaagctc cgggtacggc gtcgtctacg
     2221 ccaaaggcat ctccgcagcc tggcctgtga tgaaggaaaa tttcggcagc gcgctcttcc
     2281 tactggccag cctggggata tgcgtgtatg gaattgccgc caataaggcc cgcttgctga
     2341 acgtcctcat ccttacctgg tttattccgc tcagcctgta tgtgaccgtc ttcagccact
     2401 tcaagtatca atactggctg ccggtggcct tgcccttgtt ctcctgttgg attctggtgt
     2461 tccctgaaaa aattcgcgga ttcacgccaa aagactggcg cggcgccgtt cggattggcc
     2521 ttgcgctgct atttctgtcg caattcttca gcttcgcgct gcagagccag agcatgttct
     2581 ccgcccgcgt acaccgcgct gaaaataacc cctatatcca attttccaac accgtcgcgg
     2641 aaaagcttgc tccggttcgg ggcgtgccct tgcgcgtgta cttcgattac cgcctttacg
     2701 tccccgatac gcccggatgg aagctcagca caacctacgt cccgctcagc tacgcgcaaa
     2761 tcgccgcggg tgatttcgat attctcctgc tggcggctca gcgcgtgcgc gattacctca
     2821 gcccggaagc ggaaggggtg gaccccgcgc tcttcgctga aagccagcac ttctaccggg
     2881 acgtagaaca gaggactata gagggcttta cttttctggg cggggataac gccggcctgg
     2941 ttttcatccg gcaggggctt tgcgcgcagt atttcccggg cctgccttgc gagtgagact
     3001 gttaacttaa cgaggaatca tgaacaccga accaaagaag tttgaccgcg tccttaccga
     3061 tttcatcctt agaaatctga acctgttttt tattgtgggg atttctttgc tgacgctggt
     3121 cattcgcaac gagttccgcg gctggcagtc cggggactat ctacagcacc ttcttccctg
     3181 ggcgaaatac ctcgaagcgc acggcggctt cgccggcatc gcttccggcc cgcccaactg
     3241 caactacccg gtgacctatc agtacatcct ggcattcctg acttacctgc ccggttctta
     3301 ccttgccaag atcaaagtgg tttcggtctt cttcgatttt gtcagcgccg cctttgtgat
     3361 gctcattgtc cgggaaattg gcggctacgc caaaaagagc tttgtcccct ggctggccta
     3421 cgcgctcgcg ctgctgattc ccacgcccat cctgaacagc gcggcctggg ggcagtccga
     3481 ttcgatctac accgctttcc tggtgatttc gctctacttc ctcatccggg aaaaataccc
     3541 gctggcattc atcgccttca gcctggcgtt tttgttcaag ctgcaagcgg tcttcttctt
     3601 cccggtattt ctcctgctct atctgaaaaa ccgtaagttc agcctcctgc acttcctgat
     3661 tatcccggct gtcattgctg tgctcagcct gcccgcgatt atcattggca agccgctggc
     3721 ggaaattttc ggggtatata tcggtcaggc ccagtcgtat ccaaagacaa ccttgaattt
     3781 tcccaatatg tatttcttcc tgccggattt gcactggcag ttcgctgtcc ccgggattct
     3841 gctgaccatc gcgctgatcc tgaccctcta ttacgtgctc ctaaaacgag agcgcgttgt
     3901 gctcgcgggc gggacgctgc tcgaagtcag cctgttgacc gtgatgattt gcgcctactg
     3961 cctgccctca atgcaccagc gctacctctt cactggggat atcctggcgg tgatatacct
     4021 ctttacccgc aaagaacgcg cctatatccc cctggtcatc tggctcatca gcgtcaacgg
     4081 ctacctgtgg tacctctatg gttttggccc gctggtggag cagcgctacc tggcaatggc
     4141 gtatttgtgg atcatggtgc tcatcgcccg cgatctggtc ttcaggccgc tgaacccacc
     4201 ccaaagcttg aaagaggaca acccgcccaa tttgtaggat taccatacat cttggacagt
     4261 gaaggataag aaaaggaaag ca
//
LOCUS       DAOQAD010000134         4155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_27968_length_4155_cov_6.295366, whole genome shotgun sequence.
ACCESSION   DAOQAD010000134 DAOQAD010000000
VERSION     DAOQAD010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27968_length_4155_cov_6.295366"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..1355)
                     /locus_tag="PKL60_08260"
     CDS             complement(363..1355)
                     /locus_tag="PKL60_08260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNW96165.1"
                     /translation="MSVRAAKGDLLHDRYRIDRIIGQGGFGSIYLAEDTRLSGRFCAV
                     KQVGYDASLPPKLLEESREQFLKEATVLARLDHPNLPKVSDYFSIGDDDYLVMDYVPG
                     DDLRALVSKAAAEKRFLDEEDVLNWAVQIGDALEYLHAQQPPIVHRDIKPSNLKLTPT
                     GLVKLVDFGLVKLLAPGEVTITIMQGQGSAIYTPLEQYGGDSLHTDRRADIYAFGGTL
                     YHLLTNQAPVNVRDRFLDPKSLPAPRSLNPAISARVEEAVLWALQLHPDDRPADVRTF
                     LKALTGESTNHLRGGLVGNGAKRAERLGEADWRLVAVSAGLGFLTLLLTLLRNL"
     gene            1486..2130
                     /locus_tag="PKL60_08265"
     CDS             1486..2130
                     /locus_tag="PKL60_08265"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNW96166.1"
                     /translation="MEYAVLLAQSGPLSGQQWAVRGMLTIGRDEGCQVVIPDRQVSRQ
                     HARLSPRDNAVFLEDLNSKNGTYLNGQLLTAPTQLRESDEIRVAFAQTFLFLSSEATL
                     PLSELPGEYLQLFTLQVDADARRVWVRGEEITPPLSAQQFSLLNTLFSKSGEVVSRED
                     LIRAVWGDETPWVTEQAFDALVRRLRDRINQIDPDYDYIVTVRGHGLRLENQPH"
     gene            complement(2127..3491)
                     /locus_tag="PKL60_08270"
     CDS             complement(2127..3491)
                     /locus_tag="PKL60_08270"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HNW96167.1"
                     /translation="MPKTTVPCPRCRTPITAEITRLFDLSSDPQAKEKLLSGSANLIN
                     CPVCHYQGVYPSPIVYHDADKELLLTYFPPEANLPVPEQEKIIGPLIKKVVDTLPPEK
                     RKGYLFRPQTMLTQQLLFETILKADGITPEMMKAQQEKLTLISQLAAAAPEKLPELIA
                     QNDSKIDEQVFMLVSRLAEASAAGGDQEGVQILVNLQRGMLEHSSYGRSLAEQARESQ
                     AAVKSLQELSQKGLTRESLLDLIIESANSDARLTTLVTMARGGLDYTFFEQLSAKIDA
                     SSGEEQAKLSALREKLLQLTAAVDAALKAQMDQAQALLEEILQSDNIEEAAEKALPRV
                     DQAFADAVNHAAERAQADKDQAKLEKLMRVIAVVQAASQSGAALQLIEILLQAENETA
                     RQQIFEKAGDAIDAEFLQMLNELIGQMEKNHEQPEAVARLKDISREALRFSMQRNFAK
                     ENPA"
BASE COUNT          847 a         1200 c         1163 g          945 t
ORIGIN      
        1 cgctgagcgg gatgctgttt ggctggttgg gcgcgctggt gtggtggtac gcgaaaaaaa
       61 cggctgtgcg ggggaagtaa tttgtagaaa tcccgaagcc tgcagcgcaa ggttgtaaag
      121 cagcgtgtgc ggaagtttgg tctttgcgaa agcctgatta ccggtttgcc attgcgctga
      181 ctcgctttgt cattctgaac gcagtgaaga atctggtctt tccatagcaa gacctaccgc
      241 agtctcaggc tgacgaggcg ttctgtgatg gtgtacgagg agagacaatc tccctgccag
      301 cccacgggtc gatttcctgc ccagcgggca gcgcttcttc ccgcgcgacg tcgaccccca
      361 gcctacaggt tccgcagcaa ggtcagcagc agggtcagga atcccagccc ggcagaaact
      421 gccaccaggc gccaatccgc ctcgcccagg cgctcggcgc gcttggcgcc gttgccgacc
      481 aagccgccgc gcagatggtt ggtggattcc ccggtcaggg ctttcaaaaa ggtgcgcaca
      541 tccgcagggc ggtcatccgg atgcagctgc agcgcccata aaaccgcttc ttccacccgt
      601 gcggaaatgg cagggttcag agagcgcggc gcgggcaggc ttttcgggtc cagaaaacgg
      661 tcgcgcacat tgaccggcgc ctggttggtg agcaagtggt agagcgtccc cccgaaagcg
      721 tagatatccg cccgccggtc ggtatgcagg ctgtcaccgc cgtactgctc cagcggggtg
      781 taaatagcgc tgccctgccc ctgcataatg gtaatcgtca cttcccctgg cgccagcagc
      841 ttgaccagac caaagtccac cagctttacc agcccggtag gggtgagctt cagattggag
      901 ggttttatat cccgatgcac aataggaggc tgttgagcgt gcaggtactc cagggcatcc
      961 ccaatttgga cagcccaatt caaaacatct tcctcgtcca aaaagcgctt ctcggccgcc
     1021 gctttggaca ccagcgcgcg caggtcgtcc cccggtacgt aatccatcac cagatagtcg
     1081 tcatcgccga tggaaaagta atccgaaacc ttgggcaggt tgggatggtc cagcctggca
     1141 agcacagtgg cttccttcag gaactgttcg cgggattcct ccagcaactt gggcgggagc
     1201 gacgcatcat agccgacttg cttgaccgcg cagaaacgcc cgctgaggcg ggtatcttcc
     1261 gccagataga tgctgccgaa tccgccctga ccaatgatgc ggtcaatgcg atagcggtca
     1321 tgcaaaaggt cgcctttggc ggcgcgaacg gacatgggtg cttctctttc caaaactacc
     1381 tgcgtgctcc cttctttcgg cgtctgggag tatttggcac ggagccacgc aaaatcgcgt
     1441 ggattataat aaagaattat agcgcataag tctgaagggt tccgaatgga atatgctgtt
     1501 ctgcttgcac aatcaggtcc gctctccggc caacagtggg ctgtccgcgg catgctgacg
     1561 atcggccggg atgaaggctg ccaggtggtc atcccagacc ggcaggtctc ccgccaacat
     1621 gcccgccttt cgccgcgcga taacgccgtc ttcctcgaag acctgaacag caaaaacggc
     1681 acctacctga acggtcagct tctcaccgcc ccgacgcagc tgcgcgaaag cgatgaaatc
     1741 cgggttgcct tcgcccagac tttcctcttc ctctcatccg aagccactct gccgctctcg
     1801 gagctgccag gcgagtacct gcagctcttt accctccagg tagatgcaga cgcccggcgg
     1861 gtttgggtgc gcggggagga gattacgccg cccttgtccg cgcagcagtt cagcctgctg
     1921 aatacgctct tcagcaaaag cggagaggtg gtcagccgcg aggacttaat cagagcggtg
     1981 tggggcgatg aaaccccctg ggtgacggaa caagcctttg acgcgctcgt ccgccgcctg
     2041 cgcgaccgaa ttaatcaaat tgaccccgac tatgactata tcgtcacggt tcggggtcac
     2101 ggtttacgtc tggaaaatca gccgcactaa gcggggttct ctttggcgaa gttgcgctgc
     2161 atggagaagc gcagcgcctc ccggctgata tctttcaaac gcgccactgc ttccggctgc
     2221 tcatgattct tttccatctg cccgatgagc tcgttcagca tctgcaggaa ttccgcgtca
     2281 atcgcgtcgc cggctttttc aaaaatctgc tggcgggcag tttcattttc cgcctgcagc
     2341 agaatctcaa tcaactgcag cgccgcgccg gactggctgg cagcctgcac caccgcgatg
     2401 acccgcatca atttctccag cttcgcctgg tctttgtctg cctgggcacg ctcagcggca
     2461 tggttgaccg cgtccgcgaa agcctggtcc acgcgcggca gggctttttc cgccgcctct
     2521 tcgatgttat ccgactgcag gatctcctcc aaaagcgcct gcgcctggtc catctgcgcc
     2581 ttcagcgcgg catccaccgc cgcggtcaac tgcaagagct tttcccgcag tgcgctcaat
     2641 ttggcctgct cctcgccgct ggaagcgtca attttggcgc taagctgttc gaagaaggtg
     2701 taatccaggc ctccgcgcgc catcgtcacc agcgtggtca gccgcgcgtc agaattcgcg
     2761 gattcgatga tcaggtccag caacgattca cgcgtcaggc ccttttgact gagttcctgc
     2821 aggcttttga cagcagcctg agactcgcgc gcctgttcag ccaggctcct gccgtagctg
     2881 gaatgttcca gcatcccgcg ctgtaaattt accaggattt gcacgccttc ctggtcgccg
     2941 ccagcagcgg aagcttccgc caggcgcgag accaacataa agacctgctc atcgattttg
     3001 ctgtcgttct gtgcgatgag ttccggcagc ttctccggcg cggcagccgc cagctgcgaa
     3061 atcaacgtca gcttttcctg ctgggctttc atcatctcag gtgtgatgcc gtcagccttc
     3121 aggatggttt cgaataatag ctgttgggtc agcatcgtct gcgggcggaa cagataacct
     3181 tttcgctttt caggcggcag ggtatccaca acctttttta tcaagggtcc gatgattttt
     3241 tcctgctccg gcacgggcag gttggcttcc ggggggaagt aggtcaacag cagttcttta
     3301 tccgcgtcgt ggtatacaat cggagagggg tatacgccct gatagtgaca cacagggcag
     3361 ttaatcaggt tggcggaccc ggaaagcagc ttttctttgg cttggggatc gctgctcaaa
     3421 tcaaacaggc gggttatctc ggcagtgatt ggagtgcggc agcgcgggca tgggacagtt
     3481 gttttcggca tatgagattc gtttccttta ttttcaattc attggattat aacatccgct
     3541 cctttctctt ccgggctgtg tatagcagcg ttggggtaag attgcctcgc gatctaacgc
     3601 ttccacttca atgcccggcg ggtctgcatg gacggacgcg gcacaccatt cgatctgctg
     3661 ccagacaagc ctgctcagcc tcaccggaca aaccaatcca gggctttcct ggcattataa
     3721 gctttactga ttgacgtttc ctttgatttg tgttatctaa ttaagaggtg ttaatctgtt
     3781 attaacacgc atacccgcca agaatatttc cagttattcc gccacgcgga gccacttaac
     3841 aagggattta ttggaatatg ctaaatcctt gacatagaac tccggtttgg ggggataata
     3901 tgaaatagat tggttcgcat agaagggagt gagaaatatc cgtagagcaa caatagtttt
     3961 gcgcgttagt cacctattcc gattcaaaat tacttaggag aatgagcatg aaagtcagaa
     4021 gatttgtttg gatatttgtg agcgttattc tcgcagccag cctcgccctt gcaggctgcg
     4081 ccacccagca ggctgcaacc gaagaacccg ccgctggtga aaaactggca gtcggcattg
     4141 tgctgccaac caagg
//
LOCUS       DAOQAD010000135         4087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_28648_length_4087_cov_6.272321, whole genome shotgun sequence.
ACCESSION   DAOQAD010000135 DAOQAD010000000
VERSION     DAOQAD010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28648_length_4087_cov_6.272321"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKL60_08275"
     CDS             <1..456
                     /locus_tag="PKL60_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96168.1"
                     /translation="LVEELKSAEFLVFGVPVYIQLPSAFQSVLNRLVPMMDPGSAVFV
                     NGRTRAKLRAEYALRRTALVAVGGWWEVENLALVDRIITELSADSGVIYAGAVLRPHA
                     YALQSKRVTDEAKKEVSEAVKQCGQDLVATGGMNPPLLEMVSRPLIPWE"
     gene            complement(463..1725)
                     /locus_tag="PKL60_08280"
     CDS             complement(463..1725)
                     /locus_tag="PKL60_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96169.1"
                     /translation="MTQLTFRLTLTLGLLLLLLSSSSAALWDSTQRMREFSREVEFDF
                     TEWTAQALFQKASFAALRAEKFLNADQQNLLLQTYLEQVGATAQWSAQLDQAVSQPKL
                     ANRAAVIASTRQELTRAKIRLAQLSPLTESVVQSRTESLLLELGFGLGGQVLPPVLYQ
                     VTDLPLNLIVSPREEIRTLFSTNLIAGLDTLQKEAIESGIYEKYKVSALVEPIGGLGA
                     YPTMVMRTTDFNWLTETVAHEWIHNYLSFTPLGMRYDASAQMRTINETVASLVGKEIS
                     RQMIARDFPQYTLPLPGPRMRSRLPEESQEDAGAFNFRAEMRTTRVRVDELLAEGKVD
                     EAEAYMEARRQFFWEQGYQLRKLNQAYFAFYGSYNDQPGGGASGSDPVGPLVRRLWAR
                     SPDLKSFIAAVRGVRSFEDLQGVLAEQP"
     gene            1856..2317
                     /locus_tag="PKL60_08285"
     CDS             1856..2317
                     /locus_tag="PKL60_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HNW96170.1"
                     /translation="MHKSDYLNVLQQRWQEFLDSIAGLSPEQMKLPGVCGTWSVKDII
                     GHVTSWEQETLSTLPAILAGGSAPVYSQLYGSIDALNAYMVLGKNRQDLDEVLENLHK
                     VHAKLLKFLEEVDPAQFSEETPFLVRLSMDTSEHYPEHGAAVLSWRAKQGL"
     gene            2322..2696
                     /locus_tag="PKL60_08290"
     CDS             2322..2696
                     /locus_tag="PKL60_08290"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNW96171.1"
                     /translation="MQNAQDAPKTIPEYIAALPAEKQAVLNIVLEAVRAAAPGAQEKL
                     SYGMPAFFLDGILVYVGMQKRHVGFYPTPEAVAFFAGELRGFKTSKGAVQFPLDQPVP
                     VELVKKIVRHRIEVNRAAIAAA"
     gene            2788..>4087
                     /locus_tag="PKL60_08295"
     CDS             2788..>4087
                     /locus_tag="PKL60_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96172.1"
                     /translation="MKKLSTNTIIILIIILLALLCACIACIFTITGSTLLNRWGFDLE
                     TSLPTQPGSVEQTPLPELLQEALDEDIPQSDLVLAQANLAALKEEVVPINDPVDLAER
                     LGGKTDVPSTLPDLAAPYEVGDRKTFWVSNSDTNENFQVEAVLRFRGDNIYFWIEDGV
                     SYRESDLTRLAETFDQEIIPTNREFFGMEWNPGVDNDPRFYVLYAGGLGSSIAGYFSS
                     ADEVHPEAHPYSNAHEMFLMNSDAVSLWEDYIYGTMAHEFQHMIHWYQDRNEETWVNE
                     GFSMLAEHVNDYDAGGFDYDYLLSPDLQLTDWGAETSENGPHYGASYLFMTYFLDRFG
                     EKATKALVAHKENGFISLEAVMRELNIINPSTGAVYSADEVFMDWAAANYLLDPNLEN
                     GRYDYRSYSPSHARASSSLSACPASIISDVFQYGVDYIEIS"
BASE COUNT          891 a         1196 c         1157 g          843 t
ORIGIN      
        1 cttgtagaag agctgaagtc cgcggaattt ctcgtttttg gggtgccggt atacatccag
       61 ctcccttcgg ctttccaatc cgttctgaac cgcctcgtgc cgatgatgga cccgggctca
      121 gctgtcttcg taaatggcag gacccgcgcc aagctgagag cggaatacgc gctcaggcgc
      181 acagcgctgg tcgcggtggg tggttggtgg gaagtggaaa acctggcgct ggtcgaccgc
      241 atcatcactg aattatcagc ggatagcggc gttatctacg ctggcgcggt tctgaggccg
      301 cacgcctacg ccctgcaaag taagcgggta acagacgaag caaaaaagga agtgtcagaa
      361 gcggttaagc agtgcggtca ggacctggtc gccaccggcg gaatgaaccc cccgctgctc
      421 gagatggttt cccggccgtt gataccctgg gagtgaaaga cgctaaggct gctctgccag
      481 cacgccttgc aggtcttcaa aagaacgcac cccacgcacg gcagcgatga agcttttcag
      541 gtcggggctg cgtgcccata atctgcgtac cagcggtcct accgggtcgc tgccggaagc
      601 gccgccgccg ggctggtcgt tataagagcc gtaaaacgcg aagtatgcct ggttcagctt
      661 gcgcagctgg taaccctgct cccaaaagaa ttgacggcgg gcttccatat aggcttcagc
      721 ctcgtccact ttcccctcgg ccagcagttc atccacacgc acgcgcgttg tgcgcatttc
      781 cgcacgaaaa ttaaacgccc cggcatcctc ctggctttct tccggcaggc gcgagcgcat
      841 tctcggaccc ggcagcggga gagtatactg agggaagtcg cgcgcgatca tctgccggct
      901 gatttccttt ccaaccaggc tggcaactgt ctcgtttata gtgcgcatct gcgcgctcgc
      961 atcgtagcgc atacccaggg gtgtaaaaga aaggtaattg tgaatccact catgcgcaac
     1021 ggtctccgtc agccagttga aatctgttgt gcgcatgacc atggtcgggt aagctcccag
     1081 cccgccaatc ggttcgacca atgcggaaac cttgtatttc tcgtagatac cgctctcgat
     1141 ggcttccttt tggagcgtgt ccaacccggc gatgaggttg gtggagaaga gtgtgcggat
     1201 ttcctcccgc ggggagacaa tcaggttcag cggcaaatcc gtcacctggt agagcaccgg
     1261 tggaagcacc tgcccgccca gcccaaaccc gagctcgagc agcaggcttt ccgtgcggga
     1321 ctgcaccaca gattcggtca gcggggaaag ctgcgccagg cgtattttcg cgcgcgtcag
     1381 ctcctggcgc gtgctcgcga tgactgccgc gcggttcgcg agcttcggct gggaaactgc
     1441 ctggtcaagt tgggcgctcc actgggcagt tgcgcccacc tgttccaggt aggtctgcaa
     1501 aagcaggttt tgctggtccg cgttcaggaa tttttccgct cgcagcgctg cgaacgatgc
     1561 tttctggaaa agcgcttgcg ctgtccactc ggtgaagtcg aattcgactt cacggctgaa
     1621 ttcccgcatc cgctgggtag agtcccaaag cgcggcggaa gaggaggaga gcagcagcaa
     1681 tagcagccca agcgtgagcg tgagcctgaa agtaagctgt gtcaaattca cctggtacga
     1741 agttttcacg cacccatttt agcacgccag ctcaggcgcg cttctcagaa gcttacctca
     1801 cgcctgaaat caggctgcaa aacagtataa tttgtcacga accagaaagg gactcatgca
     1861 caaatctgat tacctgaatg tgctccaaca aaggtggcag gaattcctgg attcgattgc
     1921 cgggctcagc ccggagcaaa tgaagctccc gggagtttgc ggcacctggt cggtcaaaga
     1981 catcatcggg catgttacaa gctgggagca ggagacactc tccaccctgc cggcgatctt
     2041 ggcgggcggg tcagcgccgg tctattccca actctacggc agcattgacg cgctgaatgc
     2101 ctatatggtg ctgggtaaaa accgacagga cctggacgaa gtgctggaaa accttcataa
     2161 agtccacgcg aaactgctaa aattcctgga ggaagttgac ccggcgcagt tttctgagga
     2221 aaccccgttt ctcgtccgcc tgagcatgga tacctccgag cactacccgg agcacggcgc
     2281 tgccgtcctt tcctggcggg caaagcaagg gctgtaagca tatgcaaaac gcccaggatg
     2341 cacccaagac gatacccgaa tacatcgctg ctcttccagc tgaaaaacaa gccgtgctga
     2401 acatcgtctt ggaggcagtc cgggcagccg ccccgggcgc gcaggaaaag ttgtcttacg
     2461 ggatgccagc tttctttctg gacggtattc tggtgtacgt cggcatgcaa aagcgccacg
     2521 tcggcttcta cccgacgccg gaagccgtgg cgttcttcgc cggagagctg cgcggcttca
     2581 aaacttcaaa gggagcggtg caattcccgc tcgaccaacc cgttccggtc gagctggtga
     2641 agaaaattgt ccggcaccgc atcgaggtta accgcgctgc aattgccgcg gcttgactgc
     2701 gcacaggccg atcggggctg cagctgcccg tttgcagtat aatttcacgc aaggtgctaa
     2761 ggaatagcag cagccaggga gccatgcatg aaaaagcttt ctaccaacac catcatcatc
     2821 ctgatcatta tcctgctggc gcttttgtgc gcctgtatcg cctgcatttt caccatcaca
     2881 ggcagcacgc tgctcaaccg ctggggcttc gaccttgaaa ccagcctgcc tacgcagccg
     2941 ggctccgtgg aacagacacc cctgcctgag ctgctgcagg aagcgctgga cgaagacatc
     3001 ccacaatcag atttggttct ggcacaggcc aacctggccg cgctcaaaga ggaagtcgtt
     3061 cccatcaatg acccggtgga cctggcggaa cgccttggcg ggaaaaccga cgtgccttcc
     3121 acgctgcccg acctggccgc gccgtatgag gtcggagacc gtaagacctt ttgggtctcg
     3181 aactccgaca ccaacgaaaa cttccaggtg gaagccgtct tgcgcttccg cggcgacaat
     3241 atttatttct ggattgaaga cggcgtttcc tatcgcgaat ccgacctgac gcgcctggca
     3301 gaaacttttg accaggaaat catcccgacc aaccgcgaat tcttcgggat ggaatggaac
     3361 ccgggcgtgg ataacgaccc gcgtttttac gtgctgtacg ccggcggctt gggctcgtct
     3421 attgccgggt atttctcttc agcggatgaa gtgcacccgg aagcgcatcc ttattccaat
     3481 gcgcacgaaa tgttcctgat gaattctgat gcggtaagct tgtgggaaga ttatatctac
     3541 ggcacgatgg cgcacgagtt ccagcatatg attcactggt accaggaccg caacgaggaa
     3601 acctgggtca acgaaggctt ttccatgctg gcggagcatg taaacgacta tgacgcgggc
     3661 ggttttgatt acgattacct gctgtctccc gacctgcagc ttacggattg gggcgctgaa
     3721 accagcgaaa acggtccgca ttacggcgcc tcctacctct tcatgaccta cttcctggac
     3781 cgcttcggag aaaaagccac caaagcgctg gtcgcgcaca aagaaaacgg ctttatcagc
     3841 ctggaagcag tgatgcgtga actcaacatc atcaaccctt ccacgggcgc ggtctacagc
     3901 gctgacgaag tctttatgga ctgggctgcc gccaactacc tgctggaccc aaacctggag
     3961 aacggccgct acgattaccg cagctattcg ccttctcacg cgcgcgccag cagcagcctg
     4021 agcgcctgcc ctgcatctat tatcagcgat gtgttccaat acggcgtgga ttacatcgaa
     4081 atcagct
//
LOCUS       DAOQAD010000136         4071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_28807_length_4071_cov_5.611056, whole genome shotgun sequence.
ACCESSION   DAOQAD010000136 DAOQAD010000000
VERSION     DAOQAD010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28807_length_4071_cov_5.611056"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1082)
                     /locus_tag="PKL60_08300"
     CDS             complement(177..1082)
                     /locus_tag="PKL60_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNW96173.1"
                     /translation="MDSQQRIKQTNFSVGLGLGANIVLALVKSIVGIIGSSPALLADG
                     INSSSDVVYYLVVSVFVRAANKPADDEHPYGHTQYESVGALVVGAFVITTALTIFWNA
                     IDTLVQVLQGKSEFTGGSPLALYVALGTVLIKIALFLITARVGKITNNETVIALASDH
                     RNDILAASAATLGIFMGRLGYLWMDPAAGGLVAFFILRTGISIVRESTAALMDTVPGK
                     PLNDKVMGLVRAVPGVQEVDNILAHRFGQYYIINLTISVSDQISVHDGDMIASQVEKA
                     LLDSLPDLRSVHVHYHPRAQFRGEL"
     gene            complement(1208..2203)
                     /gene="trpS"
                     /locus_tag="PKL60_08305"
     CDS             complement(1208..2203)
                     /gene="trpS"
                     /locus_tag="PKL60_08305"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNW96174.1"
                     /translation="MPPISLTGIKPTGNPHIGNYFGMYKSALALTQKYLGMYFVADLH
                     ALTTTPDRKELQGLIYEVAASWIALGLDVEKSIFFRQSDIPEIPECTWLLSCVTPKGL
                     LNRAHAYKAAVDKNLEAGRDPDDGVNAGLYNYPLLMAADILLYNSDVVPVGLDQKQHI
                     EIARDIAENFNRIYGKTLKLPEGLISEDVQSVPGIDGRKMSKSYNNTLPIFAPEKDLR
                     KQIMRIVTDSTPPEEPKNPDTCNLYQIYRLFAPPERLAEVRGLYLQGGLAYGALKAEL
                     AELMLAYFAEARAKYTALIADRAYLDQVLAQGAERARAIARPVLLRMRKAVGIDA"
     gene            2464..3195
                     /locus_tag="PKL60_08310"
     CDS             2464..3195
                     /locus_tag="PKL60_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96175.1"
                     /translation="MKHLLRIILALGAVLFLGSMIVSPTQAQGSGSIWTTRNTCGDPD
                     QDVNLYEVGETVYINGNNFDPGSYPWDIVGQPGGASGDPGQTVASGTVTVGDDGAFCF
                     GAYTVAADDWGTYNVSVGNKSDNYRVNQQPTSTLPPTATQTATNTPTPTTTATPTTTA
                     TPTQTPTGTLTPSVTPTNTLTPTITLTPTITLTPTEELPTPTPVPTEAPRTGGLVGPG
                     ILVAAGTLFALVSAGSFIMLKKKGE"
     gene            3188..4069
                     /locus_tag="PKL60_08315"
     CDS             3188..4069
                     /locus_tag="PKL60_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96176.1"
                     /translation="MNKLNRKLIRGIGLGSGVIAIILGILLFRQYHPQVFNSTPVTAV
                     PVDPFLAASLSGMAEYNSAHSTPASSREPGTKAYRIPNAEPVPSLTPPWMEFSGLSLL
                     GSPVLMQFMPTCGDGEVVLPEFQVLPWRPDILTSGEFDLSMGTAVSWEHLGYTGLWMH
                     SGLDWLGHPLSAFALQEYLERHESGRLNTPEEFDLKAAECLIGSKVMIQIGGEQVTGK
                     VSAVARVPAEGVPEVSGHVMDLVPFLAETYPESGFAGLEEGSLVLYFCGRQLSTEAAE
                     MTAGYYSQTRIIVGITP"
BASE COUNT          872 a         1095 c         1213 g          891 t
ORIGIN      
        1 aatttgcctt attgctggca gggagatcgc ttcgtgcctc gcgatgacga aaaaatgctg
       61 cggctgcttg cgcgcgaagt ttgtaggttg ggtgcagcga cgcaggagcg aaacccaacc
      121 gcaacagtct ggaaacctca ggcaaccagc ggactgtgca ggcagcggtg gagggcttaa
      181 agctcgccgc ggaactgcgc gcgcgggtgg taatgcacgt gcacgctgcg caagtccggg
      241 agactatcca acagggcctt ttccacctgg ctggcaatca tgtccccgtc gtgcacgcta
      301 atctggtcgc tcacgctgat ggtcaggttg attatgtaat actgcccgaa gcggtgcgcg
      361 aggatattat caacttcctg cacgccggga actgcgcgca ccaatcccat taccttgtcg
      421 ttcaagggtt tgccgggaac ggtgtccatc agcgcggcgg tcgattcccg cacaatggaa
      481 atgccggtgc gcaggatgaa gaacgcgacc aacccgccgg cagcggggtc catccaaagg
      541 tagcccagcc tgcccatgaa gatgcccagc gtggcagccg aagcagccag aatatcgttg
      601 cggtggtccg aagccagcgc aatcacagtc tcgttgttgg ttattttgcc gacgcgggcg
      661 gtaatcaaaa acagcgcgat tttgatgagg acagtgccca gcgcaacgta gagcgccagc
      721 gggctgccgc cggtaaattc gcttttgcct tgaagcacct ggaccagggt atcaatggcg
      781 ttccaaaaga tggtgagcgc ggtggtaatc acgaacgcgc ccacgaccag cgcgccaacg
      841 ctctcgtact gggtgtgccc gtaggggtgc tcgtcgtccg cgggtttgtt ggcggcgcgc
      901 acaaaaaccg acaccaccag gtaatacacc acatccgagc tggagttgat gccgtcagcc
      961 agcagcgccg gcgaactgcc gatgatgcct acgatggact tgaccagcgc gaggacgata
     1021 ttcgcgccca ggcccaggcc gaccgaaaaa ttggtttgtt tgatacgctg ttgactatcc
     1081 atcactctgg tttcctctgg gcggggttcg gtttggtgcg tttccgcgcc gtactgctag
     1141 ccgcatgtac tccgcttatt tgcttgagag cattcaaccg cttcctggtc cggaattttt
     1201 ccctgcctta cgcgtcaatc ccgacggctt tacgcatacg caggagcact ggcctggcaa
     1261 tggcgcgtgc tctctctgcg ccttgagcca atacttggtc cagataggcg cggtccgcga
     1321 tgagcgcggt gtacttggcg cgggcttccg cgaagtaggc cagcatcaac tccgccagct
     1381 cagctttgag cgcgccgtaa gcaaggccgc cctgcaggta cagcccgcgc acttctgcca
     1441 gccgctccgg cggggcaaac aggcggtaga tttggtagag gttgcaggta tccgggtttt
     1501 taggttcctc gggtggggtg ctgtcggtga cgatgcgcat aatctgcttg cgcaggtctt
     1561 tttccggggc gaaaatgggc agggtgttgt tgtaactctt gctcattttg cgcccgtcaa
     1621 tgcccggcac agactgcaca tcctcggaaa tgaggccctc gggcagtttc agcgttttgc
     1681 cgtagatgcg gttgaaattc tcggcaatgt cgcgcgcgat ttcgatatgc tgcttttggt
     1741 ccagccctac cggcacgacg tccgagttgt agagcaggat gtcagccgcc atcagcaacg
     1801 ggtaattata gagcccggca ttcacgccgt catcggggtc gcgcccggct tccaggtttt
     1861 tgtccacggc ggctttgtag gcgtgcgcgc ggttcagcag tcctttgggc gtgacgcagc
     1921 ttagcagcca ggtgcattcg gggatttcgg ggatgtcgga ctggcggaag aagatcgatt
     1981 tttccacgtc gagccccagg gcaatccaac tggcggcgac ctcataaatc agcccctgca
     2041 gttctttgcg gtcaggcgtg gtcgtcagcg cgtgcaggtc cgcgacaaaa tacatgccca
     2101 ggtatttttg ggtgagggca agcgcggatt tgtacattcc gaaatagttg ccgatgtgtg
     2161 ggttgccggt gggtttgatg ccggtcagag agatgggtgg cattaatgaa ttcctcagtg
     2221 aataattgaa gcctaattat aacccaggtt ttaaagctgc ttgacgttga agccagcgct
     2281 gaaaaggaac aggtcgaaaa caagcggaaa ttgaccgtag aaaggaatgc agatattgga
     2341 gatttcagca atttctttat atgcattctt gattttatag ctgtatctgt gataatatta
     2401 aattgttcta cgaaatttcg ttgtccgcca acaaaggttg tagccaagaa aggaatgcca
     2461 aggatgaaac atctactgag aattattctg gcgcttggcg cggttttatt cctcggatcg
     2521 atgatagttt ccccaactca agcacagggc agcgggtcca tctggaccac tcggaacacg
     2581 tgtggagacc ctgaccagga tgtaaacctc tatgaagttg gtgagactgt ttatattaac
     2641 gggaataatt ttgacccggg ctcttatcca tgggacattg tcgggcagcc cggtggggct
     2701 tctggcgacc ccggacagac ggtggcctca ggcactgtga ctgtggggga tgacggcgca
     2761 ttttgttttg gtgcgtatac agtagcagcc gatgattggg gaacgtacaa tgtcagcgtt
     2821 ggcaacaaaa gcgataatta tcgcgtcaat caacaaccta catccacgtt acccccaacc
     2881 gccacgcaga cggcaacaaa cacgcccacg ccgaccacaa ccgccacgcc gaccacaact
     2941 gccacgccta cccaaacacc cacgggcacc ttaacaccca gcgtaacccc gactaataca
     3001 ctaaccccga ctattacact aacaccgact attacactaa caccgactga agaattgcca
     3061 acccccacgc cggtcccaac cgaagctccc cgcacaggcg gacttgtagg tccaggcatt
     3121 ttggtagcag ccggaacgct ctttgctttg gtctcagccg gctcattcat catgctgaag
     3181 aaaaagggtg aataaactca acagaaaact gattcgaggc attggtctcg gctcgggcgt
     3241 aatcgcgata atcctgggga ttttactgtt caggcagtat catccccagg ttttcaattc
     3301 cacgccggtg acagcggtgc ctgttgaccc ctttttggcc gcttctcttt cagggatggc
     3361 ggaatacaac agtgcccatt caacgccagc cagctcgcgg gagccaggga cgaaagccta
     3421 ccgaatccca aacgcggaac ccgttccgag cctgacgcct ccctggatgg aattttcggg
     3481 gcttagtctg ctagggagtc cggttttgat gcagttcatg cccacctgtg gggatggcga
     3541 agtcgtgctg ccagagtttc aagtgcttcc gtggaggccg gacatcctga ccagcggtga
     3601 atttgacctt agcatgggga cagcagtctc ctgggagcac ctggggtata ccggcctgtg
     3661 gatgcactcc gggctggatt ggttgggaca cccgctaagc gcctttgccc tgcaggaata
     3721 cctggagcgg cacgagagcg ggagactgaa cacgcccgag gaatttgacc tgaaagccgc
     3781 agagtgtttg attgggagta aggtgatgat tcagattggc ggggagcagg tgacgggtaa
     3841 agtttcagcg gtggcgcgcg taccggcgga gggggtgccg gaagtctcgg ggcacgtgat
     3901 ggacctggta ccttttctgg ccgagactta cccggagagc ggctttgccg ggcttgaaga
     3961 ggggagcctg gtgctgtact tctgcggccg gcagttgagc acggaggctg ccgaaatgac
     4021 ggccgggtat tactcgcaga cgcggattat tgtggggatt acgccgtgag g
//
LOCUS       DAOQAD010000137         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_30648_length_3907_cov_5.759086, whole genome shotgun sequence.
ACCESSION   DAOQAD010000137 DAOQAD010000000
VERSION     DAOQAD010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30648_length_3907_cov_5.759086"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..955
                     /locus_tag="PKL60_08320"
     CDS             260..955
                     /locus_tag="PKL60_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96177.1"
                     /translation="MPRPPLNIPTLYAGFNAPATSFDCGLRCAPFNPRGIPFCCDICC
                     AVPVAYHEEWEYLRPRTALWHPWRGDECSSEPEDPARLEADTPAHMRLLACLGPQACE
                     REYRSITCRQFPFFPYITADDRFIGLAYHWDFEPTCWLISHLEAVSPQFRAEFVQTYD
                     QLLLDCPEDYDSYYYLSEEMRAAFLQARRRIPLLHRADGFYLLSPRSGRLSRADPAKY
                     RRFGPYRNEPAGA"
     gene            1000..1653
                     /locus_tag="PKL60_08325"
     CDS             1000..1653
                     /locus_tag="PKL60_08325"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNW96178.1"
                     /translation="MTFFSDPLTFKLAGLVSVLALLIGSLIAGFSYRGKDGERYSPLN
                     HFISELGEEGVSAKAGWFNISLMVSGLSLLVACISLGLILPGLLSRIAGVIGALCALS
                     LTLVGLFPLNLYKQHTASAMAFFRAGLAMLILFTVVIITQAEGAVRIPRFYGLAGAPA
                     IAAFACFLSLTLRRGKEAKALEAPAEKARPRVSGLSIAEWAIFLSMALWFAVLAFAL"
     gene            1830..1988
                     /locus_tag="PKL60_08330"
     CDS             1830..1988
                     /locus_tag="PKL60_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96179.1"
                     /translation="MFIAQTYPSKGLWGLNFGPARITGYVNGIPSASRMVNAGVMLVR
                     LYLPALMK"
     gene            complement(2073..2702)
                     /locus_tag="PKL60_08335"
     CDS             complement(2073..2702)
                     /locus_tag="PKL60_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96180.1"
                     /translation="MKKIGVLLFVISIVVALPVGAAASPAREEAVNAQVTLVSTVTVS
                     PYQPFQEGAFHVQEAFTGSMGTYFVYYIDDMFADMVQLVGVAWNPGAMCGYDSAQLKV
                     TCSSATAITFVTIDIDMAYTVSDYTGNVIWMGWAGNYSGYTMDYTIALNYPSPLVYLT
                     YYGDDAPVSITPTQITWHKVSATTQIQMVGYAAFSDARVYASFLPIVVR"
     gene            complement(2833..3189)
                     /locus_tag="PKL60_08340"
     CDS             complement(2833..3189)
                     /locus_tag="PKL60_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96181.1"
                     /translation="MEKTILEDKNIELHLAEMPKHSLEMMDGDSSEVSLRDKYQCSRL
                     ILLLPKGAENLTGSNAVIGGASGDWELPALVAQSALLTCIPAWMSSVAAEGTAQGQGG
                     GAAPPAGYAFALQGSQ"
BASE COUNT          784 a         1206 c         1046 g          871 t
ORIGIN      
        1 ttgcgagccc ccgctgccgg aatttcccga taagcagaaa gcccaagaag ggggcgtggc
       61 aatctgcctt tgtttttaca gttgcccgct ttgttcgaat tcccctcttc gccggttcga
      121 acacccgcct tgggcaataa cgggcagcct gtcctcctga gccgatgatg aaggatcccg
      181 ccagtcgaaa gatgcctcgc tttcgctcag catgacacaa aaagccctct ccccgacagg
      241 ttcctgatag aatcaggcta tgccccgccc gcccctcaat atcccgaccc tctacgccgg
      301 cttcaacgcg cccgcgacaa gcttcgactg cggcttgcgc tgcgctcctt tcaacccgcg
      361 cggcatccca ttctgctgcg atatctgctg cgcggttcca gtcgcctacc acgaagaatg
      421 ggagtacctg cgcccgcgca cagcgctctg gcacccctgg cgcggggatg aatgcagctc
      481 cgaacccgag gaccccgccc gcctggaagc cgacaccccc gcgcatatgc gcctgctggc
      541 ctgcctgggc ccgcaagcct gcgagcgtga atatcgcagc atcacctgcc ggcagttccc
      601 cttctttccc tacatcacag ccgatgaccg cttcattggc ctggcttacc actgggattt
      661 cgagccgact tgctggctca tcagccacct ggaagcggtc agcccgcagt tccgcgcgga
      721 gttcgtgcaa acctacgacc agctcctgct ggattgcccc gaggattacg actcgtacta
      781 ttacctctcc gaagaaatgc gcgcggcatt cctacaagcc cgccggcgca tccccctgct
      841 gcaccgcgcg gacggcttct acctgctcag cccgcgctca ggcaggcttt cccgcgcgga
      901 cccggctaaa tatcgccgct ttggccccta ccggaatgag cccgccgggg cttgagtttg
      961 gggtatattg ttatcagatc tttctaaagg agacagtctg tgacattttt ctctgatccg
     1021 cttactttca aacttgctgg cctggtcagc gtgctggcac tgcttatcgg ttctctcatc
     1081 gccgggtttt cttaccgcgg caaagacggc gagcgctact ccccgctcaa tcatttcatc
     1141 tccgagctcg gcgaagaggg cgtctccgcc aaggccggct ggttcaacat cagcctgatg
     1201 gtcagcggcc tgagcctgct ggtggcctgc atctcgctgg gcctcatcct gccgggcctg
     1261 ctctcccgga tagccggcgt gattggcgcg ctgtgcgccc tcagtctgac gcttgtgggt
     1321 ctcttcccgc tcaaccttta taaacagcat accgcttctg ccatggcgtt tttccgcgcc
     1381 ggcctggcga tgttaatcct cttcacggtt gtcatcatca cccaagcgga aggcgccgtg
     1441 cgcatcccgc gcttctacgg cttggcgggc gcgcctgcca ttgccgcgtt cgcgtgcttc
     1501 ctctcgctga ctctacgccg cggcaaggaa gccaaggctc tggaagcccc cgcggaaaaa
     1561 gcccgtccgc gcgtcagcgg cttgagcatc gcggaatggg cgatcttcct gagcatggcg
     1621 ctgtggtttg ccgtgctggc tttcgcgctc taagaagttt ctacctctca cccacaaagt
     1681 agcgcaccct ccgaggaggg tgcgctttgc acctccgaaa aatctgccgc ctaagcgctt
     1741 acttgcgcgc ctacagcact cccaccaacc tgcccgttgt gcgcgtctag cggctggaca
     1801 gcggccgggt caggaatatc tcgccggaaa tgttcatcgc ccaaacttac ccgtcaaaag
     1861 ggctgtgggg gctgaatttc ggacccgcgc gcatcacagg gtatgtgaat ggcatccctt
     1921 cggcctcgcg catggtcaac gccggggtta tgctcgtgag gctgtacttg cccgccttga
     1981 tgaagtaagc tgctttccac ggggagctgg tttctcagct ccccgttttt tgtttatcgg
     2041 acgcgctggt gagggaaaag ttatcagccg gctcagcgca caacaatcgg cagaaaagag
     2101 gcataaacgc gcgcgtcgga aaaagccgcg taacctacca tctggatttg cgtggtagcg
     2161 ctgaccttat gccaggtaat ttgcgtcggg gtgatgctga ccggagcgtc gtcgccgtaa
     2221 taggtcaggt ataccagcgg gctgggatag ttcagcgcaa tcgtgtaatc catcgtgtaa
     2281 cccgagtaat ttccggccca acccatccag atgacattcc cagtgtaatc gctgaccgtg
     2341 taagccatgt cgatatcaat cgtcacaaaa gttattgcgg tggcggagct gcaggttacc
     2401 tttaattgcg cggagtcata cccgcacatt gcaccggggt tccaggcaac accgaccagt
     2461 tgtaccatgt ccgcgaacat gtcgtcgatg taatacacga aataggtgcc catacttccg
     2521 gtaaaggcct cttggacgtg gaaggcacct tcctgaaagg gctggtacgg ggaaaccgta
     2581 acggtggaaa ccagcgtgac ctgcgcgtta accgcctctt cccgcgccgg gctggctgcg
     2641 gcaccgacgg gcagcgccac tacgatgcta atgacaaaaa gcagaactcc gattttcttc
     2701 atttttcctc ctgaaaagtg ccctcataca aaaacgtgcc taaagttgca aaacaaatct
     2761 gatattttct acatcgtgca gttcccgcgc ccaagcgtat agaagggtgc gcggtcaatc
     2821 ccaagcattg attcattggc tgccttgtag tgcaaaggca tatcccgcgg gtggcgcagc
     2881 gccgccaccc tgaccctgtg ccgtgccttc tgcggcgaca gagctcatcc acgccggtat
     2941 acaggtcaga agagcagact gtgcaaccaa agcaggtagt tcccaatccc cgctggcacc
     3001 cccgatgacc gcgtttgagc cggtgaggtt ttccgcgcct ttggggagca gtaagattag
     3061 ccgggagcac tggtacttat cccgcaagct tacctcgctg gaatccccgt ccatcatctc
     3121 gaggctgtgt tttggcattt ccgctaaatg gagttctatg tttttgtcct ccaggatagt
     3181 cttctccata tggacgcttc gctgatggtg tctgctaatt ctgaattctg agtctgcgct
     3241 aacgagttga agaggcgcaa aggcacgcta caaagaaacc actaatctct ggactttgca
     3301 ttgttcgtgt tatttttggt gaccgaactg ctgcataaag tatagacggg tgaacaggca
     3361 atgtaaagcc ccaattttat cctgtggaag agtagatgag aatgtcttgt aaaccgaggc
     3421 ggtttgaaaa gcgcagcatc ttttggctgg cggggtcctt cgcctgcggc tcagcaaggc
     3481 ggagtgccca tccttgagcg gattactttg tcagtgagta taggagaacg agtttttgtc
     3541 attctgagcg cagcgaagaa tccaggcaag aaatgcagat ccttcgcctg cggctcaaaa
     3601 tgacaaaccg tttatgctgg gtgcagaagc gcgggagcga aacctaacaa caatatccaa
     3661 aaacgaaacc cgctgcatgt ttctcaccat ccgtggaatg tcggattgcg ctgccgctcc
     3721 acctgaccta cgaccacgtc ccgtcgggtt gcgctggcgc tccacccaaa ctacgaccat
     3781 gttctgtcgg gttgcgctgc cgctccaccc aacctacgac caccttctac cgctcataat
     3841 gacagtttgt aagttgggtg cagcgacgca ggagcgtaac ccaacatccg caggcagacc
     3901 tccgcgc
//
LOCUS       DAOQAD010000138         3893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_30814_length_3893_cov_5.316571, whole genome shotgun sequence.
ACCESSION   DAOQAD010000138 DAOQAD010000000
VERSION     DAOQAD010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30814_length_3893_cov_5.316571"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PKL60_08345"
     CDS             complement(<1..978)
                     /locus_tag="PKL60_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HNW96182.1"
                     /translation="MGEELINNKNQSPKYIQLREILRSRIEEGVYLPGTAIPSESELV
                     DEFGIHRLTVRNAINSLTLEGQLKPVQGKGVFVVGKKIRHELDSMSGFRQKIREQGGT
                     SSVKMLNKVVRPAGAKFARLLEIQPEDEIFFVRRLYSINGEPYSIEDVYIPKALLPDL
                     EFMDLEVFSLFDIMEFHGHTYDHGWQTLTAYWLDAKDARWLKIDPGTPVLMFECVTRN
                     KSGKVIEYNQSFTRSEKAAFSVNFSTSFKNKYLPGSLKPGGSRYRGTEPLLVGVDLGA
                     TNIRAGLVTQNGEALDVRSSRLGSDRREDTGARATIELVETLLKENGSPG"
     gene            complement(963..1754)
                     /gene="frlD"
                     /locus_tag="PKL60_08350"
     CDS             complement(963..1754)
                     /gene="frlD"
                     /locus_tag="PKL60_08350"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HNW96183.1"
                     /translation="MSMRVAAIGDNCIDVYPKLGRQYPTGNAVDFAVNIRKLGFPTSL
                     ISVTGSDQNGRWMLDTLKAEGIDISHVRVGNGATAITYMDMNGKDRVHGQYVEGVLAD
                     LAFTPEEVDFAARHDLVHSAFWGKADGNLPGLKAHGALISFDYATQKDDPLVARTLPY
                     VDYAFFSLKPAEAEAFLKDAWKQGPKVVIGTFGMDGSAAYDGERFYYFGIYPARVENT
                     IGAGDAYMAGFLAAVLRGKSLDEALRSGAKVAAEVVEVFEPWVKN"
     gene            complement(1766..2557)
                     /gene="frlD"
                     /locus_tag="PKL60_08355"
     CDS             complement(1766..2557)
                     /gene="frlD"
                     /locus_tag="PKL60_08355"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: protein motif:HMM:NF007321.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HNW96184.1"
                     /translation="MKIAAVGDNSTDVYLQQGETYPGGNTVNVAVNIIRLGGEASYVG
                     FIGADEYGAKLKKALAAKGVDISHLSTKPGTSPYTEITLDNGNRVFGKYDEGVMEHFF
                     LSEEDKAFMCQHDLLHTSVWSRIENDLKELRECGLVISFDFSDQPQHECVDIAMPYVD
                     YAFFSFQHDSPELREFMQKCWSKGPKYVVATLGENGSLGYDGSAFVEQGIIDVPVVDT
                     MGCGDSFIAGFLYGALQGLDLKSCLQSGAESAAVTIQVNGAWPLE"
     gene            complement(2561..3289)
                     /locus_tag="PKL60_08360"
     CDS             complement(2561..3289)
                     /locus_tag="PKL60_08360"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNW96185.1"
                     /translation="MDKYESRPRDRIAEQIECYIIEHNLKPGDALPSERQLCEAWQCN
                     RMTFRAAAKRLISAGILASTPLVNYYIPEPRLVRNLQDLGSFTEWVNSLGKTLKNRCL
                     SQAVLSASPRMTERLGLTKGSEVLQIVRVRLIDAEPISVDTSIIPLERFPGIENEDFE
                     NLSIYGLYKEKYGVELGSGTEDISLTFADEWEAVQLEIPEGSACYFLNGVTYNAQNVP
                     IELFKSVTRTEKIKFISELRKEES"
     gene            complement(3310..>3893)
                     /locus_tag="PKL60_08365"
     CDS             complement(3310..>3893)
                     /locus_tag="PKL60_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNW96186.1"
                     /translation="AEEYRQKSIRYFTRSMEDALEFGTNRLFVTSGWGLLDEPREEAW
                     KRSIDSMRVLAGRAEELGVQMIVEQLQPYESNLINSCADIDRMLKEVGSEQVQCCVDL
                     VAMAVVGEELQTFFDVLGSRVQHIHLADGNPSGHYVLGDGNLPIKGYIDTLERNDYTG
                     NLTLEINDSIYWDDPHTSIERTVQVLRKMLPET"
BASE COUNT          780 a         1153 c         1043 g          917 t
ORIGIN      
        1 cccgggcgaa ccattttcct ttagcagcgt ttccaccagt tcgatggtcg cgcgggctcc
       61 ggtgtcttcc cgcctatcgc tgcccaaccg gctgctgcgc acgtcgaggg cctccccgtt
      121 ctgggtgacc agacctgcgc gaatattggt cgcgccaaga tccacgccaa ccaacagcgg
      181 ttctgtgccg cggtagcggc tgccgcccgg cttcaggctg ccgggcaggt atttgttctt
      241 gaatgaggtg gaaaagttaa cagaaaaagc cgctttttca ctgcgggtaa aagactggtt
      301 gtattcaatg accttgccgg atttgttgcg cgtcacgcac tcaaacatca gtacgggggt
      361 gccggggtca atcttcagcc agcgggcgtc tttcgcgtcc agccaataag ctgtcagcgt
      421 ctgccagccg tgatcatagg tgtgaccgtg aaattccatg atgtcaaaga gcgagaacac
      481 ttccaaatcc atgaattcca ggtcgggcag cagcgctttg ggaatgtaga catcctctat
      541 ggaatacggc tcgccgttga tggaataaag ccggcggacg aagaagattt catcttccgg
      601 ctgaatttcg agcagcctgg cgaattttgc cccggccggg cggacaacct tgttgagcat
      661 ttttacggag cttgtgccgc cctgttcccg gattttctgg cggaaaccgg acatggaatc
      721 cagttcatgg cgaatctttt tcccgaccac aaagacgcct ttgccctgca cgggtttcag
      781 ctgcccttcc agcgtaagcg agttgatcgc gttgcgcacc gtcaggcggt ggatgccaaa
      841 ctcgtccacc agttcgctct ccgagggtat cgccgtgccg ggcaaataca caccctcctc
      901 aatccgggag cgcaggattt cccgcagttg gatgtacttc gggctctgat ttttgttgtt
      961 gattagttct tcacccacgg ttcaaaaacc tccaccacct ccgcagcgac ctttgcgccg
     1021 ctgcgcaagg cttcatccag gctctttccg cgcagcaccg ctgccagaaa gccggccatg
     1081 tacgcgtctc cagcgccaat cgtgttctcc acccgcgcgg ggtaaatacc aaaatagtag
     1141 aagcgttcgc cgtcgtaggc ggcgcttcca tccatcccaa aggtccctat gacaactttg
     1201 ggtccctgct tccaggcgtc tttcaggaaa gcttcggctt cagccggctt gagtgagaag
     1261 aaggcgtaat ccacataggg cagcgtgcgc gcgaccaggg ggtcgtcttt ttgggtggcg
     1321 tagtcaaagc tgataagcgc gccgtgtgcc ttaagcccgg gcaaattgcc atccgcctta
     1381 ccccaaaaag cgctgtgcac caggtcgtgc cgggcggcaa agtccacttc ttctggcgtg
     1441 aatgccaggt ccgccaaaac gccttccacg tattgcccgt gcacccggtc ttttccgttc
     1501 atgtccatat aggtaatcgc ggtcgcgccg ttccctaccc gcacatggga gatatcaatg
     1561 ccctccgcct tcagcgtatc cagcatccag cggccgtttt gatcgctgcc ggtaacgctg
     1621 atcagagaag tcggaaagcc gagtttgcga atgttcacgg caaagtccac ggcgttgccg
     1681 gtagggtact gcctgcccag tttggggtag acatcaatac aattatcacc aatcgctgcg
     1741 acgcgcatgc tcatctcctc cgtctttatt ccaaaggcca ggcgccgttg acctggatgg
     1801 ttaccgccgc gctctcagcg ccgctttgca ggcagctctt cagatccaga ccctgcagtg
     1861 cgccgtacaa gaagccggcg atgaaagagt cgccgcagcc cattgtatcc acaacgggca
     1921 cgtcgataat gccctgctcc acgaaagcgc tgccgtcata gcccaggctg ccgttctctc
     1981 ccagggttgc caccacgtat ttcggccctt tgctccaaca tttttgcata aattcgcgca
     2041 gttcagggct gtcgtgctgg aaggagaaga aggcgtaatc cacataaggc atggcgatat
     2101 cgacgcattc atgctgcggc tggtcggaaa aatcaaatga aatcaccagg ccgcactcac
     2161 gaagttcttt caggtcgttc tcgatgcggc tccagacact ggtatgcagc aggtcatgct
     2221 ggcacataaa ggctttgtct tcttccgaaa ggaaaaaatg ctccatcaca ccttcgtcgt
     2281 atttgccgaa gacgcggttg ccgttatcca gcgtgatttc cgtataaggc gaggtgcccg
     2341 gcttggtgga gaggtgcgat atatccacgc ctttggcagc cagcgctttt ttcagtttcg
     2401 cgccgtactc gtccgcgccg atgaaaccca cataggaagc ctctccccca agccgaatga
     2461 tgtttaccgc aacattcacc gtgttgccgc caggataagt ttcaccctgc tgcagataga
     2521 catccgtcga gttatcgcca actgccgcaa ttttcatggt tcagctctcc tcttttcgta
     2581 attcgctgat gaacttaatt ttctccgtcc gggtgacaga tttgaatagc tcaatgggca
     2641 cgttttgcgc gttataggtc acgccgttca ggaagtaaca cgcgctgcct tccggaatct
     2701 cgagctgcac cgcttcccac tcgtccgcaa aggtcaggct gatatcttcc gtgccggagc
     2761 cgagttctac gccgtatttt tctttataca agccatagat cgagagattc tcaaaatctt
     2821 cattttctat cccgggaaat ctctccagcg gaatgattga ggtatccact gagatcggtt
     2881 ccgcgtcgat gaggcggacg cgcacgatct gcaggacctc gcttcctttg gtcagaccca
     2941 ggcgctcagt catgcggggg ctggcagaga ggaccgcttg gctcaggcat cgatttttca
     3001 gcgttttccc caggctgttc acccattccg taaaggagcc caggtcctga agatttcgga
     3061 ccagacgggg ttcggggatg taataattca ccagcggcgt gctcgccaaa atgccggcgg
     3121 agatgagccg tttcgctgcc gcacggaagg tcatacggtt gcactgccag gcttcgcaca
     3181 gttggcgttc gctgggcagc gcgtcccctg gcttcaggtt gtgctcgata atgtagcact
     3241 caatctgttc agcaatccgg tctcttggcc tgctttcata cttgtccaca tgcacctctt
     3301 taccggggat taagtttcgg gaagcatttt gcgcagaacc tgcacggttc tttctatgga
     3361 ggtgtgcggg tcatcccagt aaatggagtc gttaatctcc agcgtcaggt ttccggtgta
     3421 gtcgttgcgc tccagggtgt cgatataccc cttgatcggc aggttgccgt ccccgaggac
     3481 atagtgcccg gatgggttgc cgtccgcgag gtggatatgc tgcacgcggc tgccgagcac
     3541 atcaaagaag gtctgcagtt cttcgccgac cacggccatg gccaccaggt ctacgcagca
     3601 ctgcacctgt tccgacccca cttccttgag catccggtct atatcggcac agctgttaat
     3661 gaggttggat tcgtagggct gaagctgttc gacaatcatc tgcacgccca gttcctccgc
     3721 ccgcccggcc agcacccgca tcgagtcgat ggaacgcttc caagcctctt cgcgcggttc
     3781 gtccagcagt ccccagcccg aagtcacaaa caggcggttg gtaccaaatt ccagcgcgtc
     3841 ttccatggag cgggtaaagt agcggatgct cttctggcgg tattcctcag cac
//
LOCUS       DAOQAD010000139         3854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_31256_length_3854_cov_5.248750, whole genome shotgun sequence.
ACCESSION   DAOQAD010000139 DAOQAD010000000
VERSION     DAOQAD010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31256_length_3854_cov_5.248750"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PKL60_08370"
     CDS             <1..717
                     /locus_tag="PKL60_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNW96187.1"
                     /translation="GSTTVQPLAEKLAESFMSTNPDVQIDVQGGGSSVGVKAAGEGTS
                     DIGMASREIKQEELTQFPNLQVHVIARDGIAVVVHPDNPVSDLSLDQIRDIFSGKIVN
                     WSEVGGADAAITVAAREEGSGTRDAFMEMVMGKDALIVDSAILQSSNGAIRTVVSTTP
                     NVIAFLSFGYLDESTKAVSIGGAAATEENAASGVYPVVRPLNLLTNGAPEGLVKAFLD
                     FVLSPEGQKMVREEGYIPVK"
     gene            803..1696
                     /gene="pstC"
                     /locus_tag="PKL60_08375"
     CDS             803..1696
                     /gene="pstC"
                     /locus_tag="PKL60_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNW96188.1"
                     /translation="MQTKLRVDRAVRTILLISASFSILIVVSIFVFIFRSSLPAFREI
                     GLQGLLGELTWRPTNGSFGILAMVVGSVMVTLMALVIGVPLAIAAAVFLAEIAPRGVQ
                     AVVRPAVQLLAGIPSVVYGLFGMVVLVPLIRRIPVAGNSGYGLLSAAIVLAVMILPTI
                     TSISEDAIRAVPRSYKEASLALGATHLQTIFQVLLPAGKSGIVAALILGIGRALGETM
                     AMIMVIGNSVILPSPLDDNPLTIFLSQARTLTGNIAVEINYASGLHASALFATGVVLF
                     VLIMVVNISAHAILNRSNSAA"
     gene            1710..2645
                     /gene="pstA"
                     /locus_tag="PKL60_08380"
     CDS             1710..2645
                     /gene="pstA"
                     /locus_tag="PKL60_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNW96189.1"
                     /translation="MNAPEMTSTRPTELPSSRTSANLRKRQAAQKFGFGLLWLASLVV
                     ILILLVVVGYVFLTGFKSLSWEFLTTAPKGGLSGEGGISTTILTTIYLVGLILVIAAP
                     LGIGAAIFLIEYQEQTKTGGKFLRGLLQAANFGVETLAGIPSIIFGLFGYALFVSTLK
                     FGFSLLSAGLAGACLVLPTIIRTTQEALLTVPVSLREGSLALGATRWQTISKVVLPAA
                     IPGIITGIMLSVGRIISETAVFYVTLGGSYRLPNSLLSGGRTLSLHLYYLAMDTNAFE
                     KAMGTGAVLIVSIILINLVIDLISRKLSAGVRQNQ"
     gene            2642..3409
                     /gene="pstB"
                     /locus_tag="PKL60_08385"
     CDS             2642..3409
                     /gene="pstB"
                     /locus_tag="PKL60_08385"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNW96190.1"
                     /translation="MNAIKLETNAYSLFYGKFRALEDISLKIPEKQVTAIIGPSGCGK
                     STFLRTFNRMNDLIPGVRTEGQALLDGEDIFSMDVVALRQRVGMVFQKPNPFPKSIYD
                     NVAYGPRVNGIRDKKALDEIVETSLRGAALWDEVKDNLGKSGLALSGGQQQRLCIARA
                     LAVRPSVILMDEPAASLDPIATQRIEELIRQLVEEYTIVIVTHNMQQAGRVSDYTAFF
                     FTDESRTGKLVEFNLTRKIFTNPQDKRTEDYITGRFG"
     gene            complement(3539..>3854)
                     /locus_tag="PKL60_08390"
     CDS             complement(3539..>3854)
                     /locus_tag="PKL60_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96191.1"
                     /translation="EGSGVTCNALHPGIIPGTNVTRVMWLRFQTTREEGAQTIVNCCL
                     NPELAKVTGEYISIDRIARANPQALSLRFQETLWKKSLAWCGLDENGQSEFAVQSGNP
                     AS"
BASE COUNT          753 a         1106 c         1105 g          890 t
ORIGIN      
        1 ggctccacca ccgtgcagcc cctggctgaa aagctggctg agagctttat gagcacgaac
       61 ccggatgtcc agatcgatgt tcagggcggc ggttccagcg tcggcgttaa ggctgccgga
      121 gagggcacgt cggatattgg tatggcttcc cgcgagatta agcaagaaga actgacccag
      181 ttccccaacc tgcaggtgca tgtcatcgcc cgggacggta tcgcggtggt cgttcatccg
      241 gataatccgg tcagcgacct gagcctcgat caaatccgcg acatttttag cggcaaaatc
      301 gtgaactgga gcgaagttgg cggcgcggac gcggcgatta ctgtggctgc acgtgaagaa
      361 ggctccggaa cccgcgacgc tttcatggaa atggtgatgg gcaaggatgc tctgattgtg
      421 gacagcgcaa tcctgcagtc ttccaacggc gccatccgca ccgtagtttc aaccacaccc
      481 aacgtcattg ctttcctctc cttcggttac ctggatgaga gcacgaaagc cgtgagcatt
      541 ggcggcgctg ccgcgaccga agaaaatgcc gccagcggcg tttatccggt ggttcgcccc
      601 ctgaacctgc tcaccaacgg cgcccccgaa ggcctcgtca aagccttcct ggattttgtc
      661 ctcagccccg aaggccagaa gatggtgcgt gaagaaggtt acatccctgt aaagtaaatt
      721 cctgacaggt caagattcta cctggcgtcc tcgaagcctt ggggacgcca ggtttctgat
      781 aaaataaaac gctggagaag aaatgcagac aaaacttcgg gttgaccgcg ccgtcaggac
      841 gatcctgctc atttctgcat cgttttccat cctcattgtc gtttccatct ttgtgttcat
      901 tttccgctcc agcctcccgg ctttcaggga gattgggctg cagggtttat tgggggagct
      961 gacctggcgc cctaccaacg gcagctttgg catcctggcg atggttgttg gctcggtgat
     1021 ggtcacgctg atggcgctgg tcatcggtgt accgctcgcg attgccgcgg ccgtcttcct
     1081 ggcggaaatt gccccgcgcg gagtgcaggc agtggttcgc ccggctgtac agctcctggc
     1141 tggcattccc tcggtagtct atggtttgtt tgggatggtt gtgctggtgc cgctgatccg
     1201 caggatcccg gtggctggca attccggtta tgggctgctc tccgccgcga ttgttctggc
     1261 ggtgatgatc ctgcccacca tcaccagcat ctccgaagat gccatccggg ccgtcccgcg
     1321 ttcgtacaaa gaagcctcgc tcgccttggg agctacccac ctacagacca tctttcaggt
     1381 gcttttgccc gctggtaagt ccggcatcgt cgcggcgttg atccttggga ttggcagagc
     1441 cctgggcgag acgatggcaa tgattatggt gattggcaat tctgtcatcc tgccctcgcc
     1501 gctggacgac aaccccctga cgatcttcct cagccaggcc cgcacgctca ccggcaatat
     1561 agccgtggaa atcaactacg cctccgggct acatgccagc gccctgtttg caactggcgt
     1621 tgtgctgttt gtgcttatta tggttgtgaa tatatccgct cacgcgattt tgaatcgcag
     1681 caattcagcg gcctgaggat gcgaatacga tgaacgcgcc ggaaatgact tcgaccaggc
     1741 cgacagaatt gccttccagc agaacgtccg cgaacctgcg caagcgtcag gcagcccaga
     1801 agttcggttt cgggcttttg tggctggcca gcctggtggt tattctcatt ctgctggtcg
     1861 tggttgggta tgtgtttctc accggcttca aatccctgag ttgggaattc ctcaccacgg
     1921 cgccaaaagg cgggctctcc ggtgaaggcg gcatttccac cacgattctc accacgattt
     1981 atctggttgg tctgattctg gtaattgccg cgccgctcgg catcggggcg gccatcttcc
     2041 tgattgagta tcaggagcag accaagactg gcggtaaatt cctccggggg cttttacagg
     2101 cagccaactt tggcgtggaa acgctggcag gcatcccctc tatcattttt ggcttattcg
     2161 gttacgcgct gtttgtctcc acgcttaagt ttggattttc gctgctttcc gcggggctgg
     2221 ccggcgcttg cctggtgctg ccaaccatca tccgcacgac gcaggaagct ttgctgaccg
     2281 tcccggtgag tttacgcgag ggcagcctgg ccctgggcgc cacgcgctgg caaaccatca
     2341 gcaaggttgt tctgccggct gccattccgg gcatcatcac cggcatcatg ctgagcgtcg
     2401 ggcgcatcat cagtgagacg gcggtctttt acgtcacgct gggcggcagc taccgcctgc
     2461 cgaattcact gctttccggc gggcgcacgc tctcgctgca tttatactac ctggcgatgg
     2521 ataccaacgc ttttgaaaaa gccatgggaa ccggcgcggt tctgatcgtc tccatcatcc
     2581 tcatcaatct ggtcatcgac ttaatttcca gaaaacttag cgccggagtg cgtcagaatc
     2641 aatgaacgcg attaaacttg aaaccaatgc ttacagtctg ttctatggga aatttcgggc
     2701 gttggaggat atcagcctga aaatccctga aaaacaggtc acagccatca ttggaccttc
     2761 cggctgcggt aagagcacat ttctgcgcac tttcaaccgc atgaacgacc tgattcccgg
     2821 cgtccgcacc gaaggtcagg cgctcttgga cggggaagat attttttcca tggatgtggt
     2881 cgcgctcaga cagcgggtgg gcatggtttt ccagaagccg aacccctttc caaaatcgat
     2941 ttatgacaac gtggcctatg ggccgcgtgt caacggcatc cgcgacaaaa aagctctgga
     3001 cgaaatcgtg gaaaccagcc tgcgtggagc cgcgctatgg gatgaagtga aagacaacct
     3061 gggcaagtcc gggctcgcgc tttcaggtgg gcagcagcag cgtttgtgca tcgcgcgggc
     3121 gctggcggtg agacccagtg tcattctgat ggacgagccg gccgcttcgt tggaccccat
     3181 cgccacgcag cgcatcgagg aattaatccg ccaactggtg gaagagtaca ccatcgtgat
     3241 tgtgacgcac aacatgcagc aggccgggcg cgtttcggat tacactgcat tcttcttcac
     3301 ggatgagagc cgcacgggca agctggtgga atttaacctg acccgcaaaa tttttaccaa
     3361 cccgcaggac aaacgcaccg aggattacat caccggcagg ttcgggtaag taatttcgcg
     3421 cctggctgaa cccaggcaca acagggcgga ccggactgcc tgaatagcct tccatcctgc
     3481 cgggatttac atccgtggga ttggctgtgt ttttccccca ctttttatat cggcggcatt
     3541 aagatgccgg gtttccgctt tggacggcaa actctgactg gccattttcg tccagcccgc
     3601 accaggccag gctctttttc cacagggtct cctggaagcg cagtgagagc gcctgcggat
     3661 tggcgcgcgc tatccggtcg atagagatat attccccggt gactttggcc aattccgggt
     3721 taagacagca gttcacgatg gtttgcgcgc cttcttcgcg cgtggtctgg aagcgcagcc
     3781 acatcacgcg ggtcacgttg gttccgggga tgatgcccgg atgcagcgca ttgcaggtca
     3841 cgccgctccc ctcc
//
LOCUS       DAOQAD010000140         3528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_35592_length_3528_cov_7.964584, whole genome shotgun sequence.
ACCESSION   DAOQAD010000140 DAOQAD010000000
VERSION     DAOQAD010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35592_length_3528_cov_7.964584"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PKL60_08395"
     CDS             <1..334
                     /locus_tag="PKL60_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     C"
                     /protein_id="HNW96192.1"
                     /translation="KKEVIMTPDSITPEMFAKLVTLAALELTPEESEYLRTELNHQLA
                     AVKELSEIQLAEGVQPSLHGLDCQGSAPRPDMWLPFPNPAEILAQAPDSDGAYFRVPD
                     FSRGEGES"
     gene            331..1935
                     /gene="gatA"
                     /locus_tag="PKL60_08400"
     CDS             331..1935
                     /gene="gatA"
                     /locus_tag="PKL60_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNW96193.1"
                     /translation="MNSIQLSARQIAQKVRAGELSALEVLEDHLRQIQAVDGVPGSLQ
                     DLSLDPSEESKVHAFLEVSAQLAQRQAEQVQVRVQRGEDPGPLAGVPVSIKDIFCVQG
                     TTTTAASRILYNFRPPYTATTVERLQKAGALIIGKTNLDEFTYGSSSESSAFQPHPNN
                     PWNTQRVTGGSSGGSAASVAAREVPLSLGTDTGGSIRQPAAFCGVVGLKPTYGRTSRY
                     GLIAFGSSLDCPGPLARDVPDAALMLQVLAGTDPKDSTSALAPVPDYLAALQEGVRGL
                     RIGLSRDYDHISYPDTLTGEMSVQAIQPEMNSAVRDVAEALARLGAEIVEDVPMPNAR
                     HGIPAYFVISRVEAASNLHRFDGVKYGYRTSAPVRGLSELYRATRAEGFGLQPKLRIL
                     MGMYVSAAQHSAQYYQKALRVRSMIRSDFEQAFDPRGAYRLDALLTPTTPTTAFRRSE
                     VLGDSVLMQYSDQMTVAGNHAGIPAVSIPAGLDPLGLPIGVQFFGMDFREDLILRAAY
                     AYEQATQNAKWRGVIPQALRGSEVSA"
     gene            1932..3368
                     /gene="gatB"
                     /locus_tag="PKL60_08405"
     CDS             1932..3368
                     /gene="gatB"
                     /locus_tag="PKL60_08405"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNW96194.1"
                     /translation="MKTYEIVIGLETHIQLNTASKIFCACKADSWGAEPNTNICPVCS
                     GLPGVLPVLNAEAVRKGALLAAAMHAEINPVSYFDRKNYFYPDLPKGYQITQFDEPIG
                     KGGYMDLEMPDGTIRRVRIHKLHLEEDAGKTKIELGERLVDFNRCGVALVEMVTEPDL
                     RSGEEAAQYLTQLRQLLRWIGVSEADMERGHLRCDANVSIREAGSAVLNTKTEIKNVN
                     SIDAVRQAISEEAKRQIEAVEAGQRIQSYTLDWDADTGTLRKMRSKESEADYRYFREP
                     DLLPVVLSAEEIAQAQSSLPELPLARRARFIRDYQLSAYDADILTSERALSDYYEESV
                     SLCGGAAKVVSNWMMNDLSRLMNETGKSAAELRLRPADLAEIIQLVEGGKISTATGKS
                     LLAKVEAGGGKPAGIVEREGLGLIADEGALSSQIAAVLAESAAEVESFRAGKESLLGW
                     FVGQVMRRTGGKADPKRTRELLLEQLKR"
     gene            3418..3489
                     /locus_tag="PKL60_08410"
     tRNA            3418..3489
                     /locus_tag="PKL60_08410"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3450..3452,aa:Gly,seq:tcc)
BASE COUNT          716 a         1118 c         1081 g          613 t
ORIGIN      
        1 aaaaaaggaa gtaattatga cgcctgacag catcacgccg gaaatgtttg ccaaattggt
       61 cactctggcc gccctggagc tgacccccga agaaagcgaa taccttcgca cggagctgaa
      121 ccaccaattg gcagcggtga aagaactgag tgaaatccaa ctggcggaag gcgtgcagcc
      181 cagcttgcac ggcctggact gccagggcag cgccccgcgc ccggatatgt ggctcccctt
      241 tcctaatccg gcggaaatcc tggctcaggc gccggatagt gacggcgctt atttccgcgt
      301 cccggatttt tcgcgcggag agggggaatc atgaacagca tccagctcag cgcccgtcag
      361 attgcccaaa aagtgcgcgc gggggaactc tccgcgctgg aagtgctgga agaccacctg
      421 cggcaaatcc aggctgtgga cggcgtcccc ggcagcctgc aagacctaag cctggaccca
      481 tctgaagaaa gcaaggtaca cgcctttctc gaagtttccg cgcagcttgc ccaaaggcag
      541 gccgaacagg tgcaggtgcg cgtgcaacgc ggcgaggacc ccggcccgct ggccggcgtg
      601 cccgtttcca ttaaagatat cttctgcgtg cagggcacca ccaccacggc tgcctcacgc
      661 atcctgtaca acttccgtcc gccctacacc gccacaaccg tggaacgcct gcagaaagcc
      721 ggcgcgctga ttatcggcaa gaccaacctg gacgagttca cctacggctc gtcgagcgag
      781 tccagcgcgt tccagccgca ccccaacaac ccctggaata cacagcgcgt tacaggcggt
      841 tcgtccggcg gctcggcggc gtcggtcgcc gcgcgcgagg taccgctctc gcttggcacg
      901 gataccggcg gctccatccg ccaacccgct gccttttgcg gcgtggtcgg gctcaagccc
      961 acctacgggc gcacctcccg ctacggtctg atagccttcg gctcatcgct ggactgccct
     1021 ggcccgctgg cccgcgacgt cccggacgcg gctctgatgc tgcaggtttt ggcaggcacg
     1081 gacccaaagg acagcaccag cgcgctcgcg ccggtaccag attatctggc ggcgctgcag
     1141 gaaggcgtgc gcgggctgcg catcgggctc tcgcgcgatt acgaccatat cagctacccg
     1201 gacacgctca ccggcgaaat gagcgtgcag gccatccaac cggaaatgaa cagcgccgtg
     1261 cgagacgttg cggaagcgct tgccaggctt ggcgctgaaa ttgtggagga cgtgccgatg
     1321 ccaaatgccc gccacggcat cccggcctat ttcgtcatct cccgcgtgga agccgcttcc
     1381 aacctgcacc gcttcgacgg cgtcaagtac ggctaccgca caagcgctcc cgtgcgcggg
     1441 ctgagcgagc tctaccgcgc cacgcgcgcc gagggctttg gcttgcagcc caaactgcgc
     1501 attctgatgg gcatgtacgt gagcgccgcc cagcacagcg cccagtacta tcagaaagcc
     1561 ctgcgcgtgc gctccatgat tcgttctgac tttgaacagg cctttgaccc gcgcggagcc
     1621 taccgcctgg acgcgctgct cacgccgacc acccccacta ccgcgttccg ccgcagcgaa
     1681 gtgctgggtg actcggtgtt gatgcagtac tccgaccaga tgaccgtggc aggcaaccac
     1741 gccggcatcc ccgccgtcag catccccgcc gggctggacc cgctcgggct gcctatcggc
     1801 gtgcagttct ttggcatgga cttccgcgag gacttaatcc tgagggcggc ttacgcctac
     1861 gagcaggcca cccaaaacgc gaagtggcgc ggcgttatcc cgcaagcttt gcgcggcagt
     1921 gaggtgagcg catgaaaact tacgagattg tcatcggttt ggaaacgcat atccagctga
     1981 acaccgccag caagattttc tgcgcctgca aagcggattc atggggcgcg gagccaaaca
     2041 ccaacatttg cccggtttgc agcggtctgc ccggcgtgct gcctgtgctg aacgctgaag
     2101 ccgtgcgcaa aggcgcgctg ctcgccgctg ccatgcacgc ggaaatcaac ccggtctctt
     2161 actttgaccg caagaactat ttctacccgg acctgcccaa aggctaccag attacccagt
     2221 tcgacgaacc gattggcaag ggcggctata tggacctgga aatgccggat ggtaccattc
     2281 ggcgcgtgcg catccacaag ctgcacctgg aagaggacgc cggcaagacc aaaattgagc
     2341 ttggcgaacg cctggtggac tttaaccgct gcggcgtggc gctggtggag atggtcaccg
     2401 aacccgattt gcgctccggg gaggaagccg cccaatacct gacccaactg cgccagctat
     2461 tgcgctggat tggcgtgtcc gaggcggata tggagcgcgg gcatttgcgc tgcgacgcga
     2521 acgtttccat ccgggaagcc ggcagcgccg tgctgaacac caaaacagaa atcaagaacg
     2581 tcaactccat tgacgcggtc cgtcaggcga ttagcgagga agccaaacgc cagattgaag
     2641 ccgtggaagc cggccagcgc atccaatcct acaccctgga ctgggacgcc gatacaggca
     2701 cactgcgcaa gatgcgctcc aaggaaagcg aagccgacta ccgctacttc cgcgagccgg
     2761 acctgctgcc ggtggtgctc tccgctgagg aaatcgcgca ggcgcagagc agcctgccgg
     2821 agctgccgct tgcccgccgg gcgcgtttta tccgggatta tcagctttcc gcttacgacg
     2881 cggatattct caccagcgag cgcgctctct cagattacta cgaagaaagc gtgagcttat
     2941 gcggcggggc agcaaaagtg gtctccaact ggatgatgaa cgacctcagc cgcctgatga
     3001 acgagacggg caagagcgcc gccgagctac gcctgcgccc ggcggacctg gctgagatta
     3061 tccagctggt ggagggcggc aagatttcga cggctaccgg aaagagcctg ctggcgaagg
     3121 tggaagccgg cggcggcaag cccgccggga ttgtggaacg cgaaggcctg ggcctgatcg
     3181 cggatgaggg cgccctaagc tcgcaaatcg cggcggtatt ggcggagtcc gcggctgagg
     3241 tggagagctt tagagccggg aaggaaagct tgctggggtg gtttgtgggt caggtaatgc
     3301 gccgcactgg cgggaaagcc gaccccaaac gcacgcgtga gcttttgctg gagcagttga
     3361 agcgttaaga gcgccggcat tgacgccggc ctttgaattg cataaaatag attgcacgcg
     3421 ggcgtcgcca agtggtaagg cattagcctt ccaagctaac attcgccggt tcgaatccgg
     3481 tcgcccgctt ttttgtttta atttcctttt tagccggttc gaacaccc
//
LOCUS       DAOQAD010000141         3482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_36292_length_3482_cov_5.807412, whole genome shotgun sequence.
ACCESSION   DAOQAD010000141 DAOQAD010000000
VERSION     DAOQAD010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36292_length_3482_cov_5.807412"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..3286
                     /gene="uvrA"
                     /locus_tag="PKL60_08415"
     CDS             380..3286
                     /gene="uvrA"
                     /locus_tag="PKL60_08415"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNW96195.1"
                     /translation="MPSDNLRVIGAREHNLKNITVEIPRDKLVVLTGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFLGQMDKPDVDAIEGLSPAVSIDQKGTSHNPRSTVGT
                     VTEIYDYLRLLFARVGIPHCPVCGRPVQRQSAQEIVDTILALPEGSRLLILAPVVRER
                     KGTYQAVLEEISKSGFVRARVDGEVFSLDDKIQLDRYKKHTIEAVVDRLVISKPETED
                     DELAMRSRLTDSIETALRFGEGYVTVQNLSVEAPEDIQFSENLACPEHGSILIEIEPR
                     TFSFNTPHGACPTCQGLGSREEIDPDLLIPDKSLSLREGAIAALEWSGPREEGGYYWQ
                     MIEAIARENQFSLDTPVSQLSENDLNMILHGTNGQEIRVEFLGRNGRKSAFSTSFEGV
                     IGNLERRYRETQSEYIRMRISEFMGVKQCPTCQGKRLRKEALAVLVQDANIIDVTEWP
                     VNRTLAWVNDLASPDASPLSARQQAIAERILREIRARLGFLVDVGLDYLTLERSASTL
                     SGGEAQRIRLATQVGSRLMGVLYVLDEPSIGLHPRDNRRLLDTLKGLRDMGNTVLVVE
                     HDDETIRSADWVIDLGPGAGEHGGEVVAEGTPERIMAVPESLTGAYLSGRKQVPVPEE
                     RRPGNGQFLRVVGATENNLKNLSVNIPLGKFVCITGVSGSGKSTLLVDVLYNTLARQL
                     MNAHTQPGAHERIEGVQHLDKVINIDQTPIGRTPRSNPATYTGMFDDIRDLFSELPDA
                     KIRGYGKGRFSFNVKGGRCEACQGQGELKIEMQFLPDVYVPCEVCRGKRYNHETLQVR
                     FKDKSIADVLDLTVESALELFPAFPRIQRRLETLQAVGLGYVRLGQPATTLSGGEAQR
                     VKLSRELSRRATGRTLYVLDEPSVGLHAADVHKLIEVLQSLVSTGNTVVVIEHNLDII
                     KVADWVIDLGPEGGDRGGYLVAEGTPEQLCGSPASYTGQYLQKYVRQVHQRECV"
BASE COUNT          707 a         1118 c         1038 g          619 t
ORIGIN      
        1 gacgggtact ttcagtgagc ggaggggcga ggcatcctgc ggcagcgcct ttgctccgcc
       61 agacgcacac gccctggatg agtcgcccgt atcccaggat ttttcgcacg ggtctcttga
      121 atggaattaa cggatgtttg gagacctgac ggtcaaactt cacgcgcgcg gtctggaggc
      181 cggcgcgatc tgggtttgga gacctgacgc tcaagccccg cgcgcggtct ggaggccggc
      241 gcggccagag gacattatcc tgttgctctt acacacacat taacccgctg ctcttacact
      301 cattccaaaa agtgtatact tattcattgc ggcccaatca tagaacaaat ggtataatta
      361 tcggtcggag tgagaaccta tgccctcaga taacctgcgc gtcatcggcg cgcgcgaaca
      421 caacctcaaa aacatcaccg tggaaatccc gcgcgacaag ctggtcgtcc tgaccggcct
      481 gtccggttcc ggcaaatcct cgctggcatt cgacacgatc tttgccgaag gtcagcgccg
      541 ctatgtggaa tcgctctccg cttacgcccg ccagttcctc gggcaaatgg acaaacccga
      601 cgtggacgcc atcgaaggcc tctctcctgc cgtttccatc gaccagaagg gcacctcgca
      661 caacccccgc tccacggtcg gcacggtcac ggaaatctac gactacctgc gcctgctgtt
      721 cgcccgcgtg ggcattcccc actgcccggt ctgcggccgg ccggtccagc gccaatccgc
      781 gcaggaaatc gtggatacca tcctcgcgct gccggaaggc agccgcctcc tgatcctggc
      841 gccggtggtg cgcgaacgga aaggcaccta ccaggcagta ttggaggaaa tcagcaagtc
      901 cggttttgtg cgcgcgcgcg tggatggcga agtgttctcg ctcgacgaca aaatccagct
      961 ggaccgctac aaaaagcaca ccatcgaagc ggtggtggac cgcctggtta tcagcaagcc
     1021 cgaaaccgag gatgacgagc tggccatgcg ctcccgcctg acggactcga tcgaaaccgc
     1081 gctgcgcttc ggcgaaggct acgtgaccgt ccagaacctg agcgtggaag cgcctgagga
     1141 tatacagttc tcagaaaacc tggcctgccc ggaacacggc tccatcctga tagaaatcga
     1201 gccgcgcacc ttctcgttca acaccccgca cggcgcctgc cccacctgtc agggcctcgg
     1261 cagccgcgag gaaattgacc ccgacctgct catcccggat aagagcctct cccttcgcga
     1321 aggcgccatc gccgcgctgg agtggtccgg cccgcgcgag gaaggcggtt attactggca
     1381 aatgattgaa gccatcgcca gggaaaatca attcagcctg gatacccccg tcagccagct
     1441 ttccgaaaac gacctgaaca tgattttgca cggcacgaac gggcaggaaa tccgcgtaga
     1501 gttcctcggc cgaaacggac gcaagtccgc gttcagcacc tccttcgaag gcgtcatcgg
     1561 caacctggaa cggcgctacc gcgaaacgca gtctgaatac atccgcatgc gcatttcgga
     1621 gtttatgggc gtgaagcagt gcccgacctg ccagggcaag cgcctgcgta aagaagccct
     1681 ggccgtgttg gtgcaagacg cgaacatcat cgatgtaacg gagtggccgg tcaaccgcac
     1741 cctggcctgg gtgaacgatc tggcttcccc ggacgcctcc ccgctcagtg cgcgccagca
     1801 ggcaatcgcg gaacgcatcc tgcgcgaaat ccgcgcgcgc ctcggctttt tggtggacgt
     1861 cggcctggat tacctgacgc tcgagcgcag cgccagcacc ctctccggcg gcgaggccca
     1921 gcgcatccgc ctggcaaccc aggtgggctc caggctgatg ggcgtgctct acgtgctgga
     1981 tgagccctcc atcggcttgc acccgcgcga taaccgccgc ctcctggata ccctgaaagg
     2041 cctgcgcgac atgggcaaca ccgttttggt ggtggagcac gatgacgaaa ccattcgcag
     2101 cgccgattgg gtaattgacc tgggccccgg agcgggcgag cacggcggcg aagttgtggc
     2161 ggaaggcacg cccgaacgca tcatggccgt tcccgaaagc ctgaccggcg cctacctctc
     2221 cggacgcaag caagtaccgg tacccgaaga gcgccgtccc gggaacggac agttcttgcg
     2281 cgtggtcggc gcgaccgaaa acaacctgaa aaacctgagc gtgaacatcc cgctgggcaa
     2341 attcgtgtgc atcaccggcg tctccggctc cggcaagtcc acgctgctgg tggacgtgct
     2401 ctacaacacg ctcgcccggc agctgatgaa cgcgcacacc caacccggcg cgcacgaacg
     2461 catcgaaggc gtccaacatc tcgacaaggt gattaacatc gaccagaccc ccatcgggcg
     2521 caccccccgt tcgaacccgg ccacctacac cggcatgttc gacgacatcc gcgacctgtt
     2581 ttccgagctg ccggacgcga aaatacgcgg ctacggcaaa ggccgcttct cgttcaacgt
     2641 caagggcggg cgctgcgaag cctgccaggg tcagggcgag ctgaaaattg agatgcagtt
     2701 cctgccggat gtgtacgtcc cctgcgaggt ctgccgcggc aagcgctaca accacgaaac
     2761 gctgcaggtg cgctttaagg acaagagcat cgcggacgtc ctcgacctca cggtggaatc
     2821 cgcgttggaa ttattccccg cgtttccgcg cattcagcgc cggctcgaga cgctgcaggc
     2881 ggtcgggctg ggatacgtgc gccttggcca gcctgccacc acgctctccg gcggcgaagc
     2941 ccagcgcgtc aagctctcca gggagctttc ccgccgcgcc accggccgga cgctgtacgt
     3001 gctggatgag ccttcggtcg ggctgcacgc cgcggatgtg cataaattga ttgaggtgct
     3061 gcagtcgctg gtcagcacgg gcaacaccgt ggtggtgatt gaacacaacc tggatatcat
     3121 caaggtggcg gattgggtga ttgatttggg cccggaaggc ggcgaccgcg gcggatacct
     3181 ggtggcggag ggcacccccg agcagctgtg cggcagcccg gcttcctata ccggtcaata
     3241 cctgcagaag tacgtccgcc aggtgcacca gcgcgagtgc gtgtaaggca ctgtccggcc
     3301 cggcaggcgt atattagtca tcgcgacaaa tcgggcggca ttacgagccc gtgaagcgat
     3361 taccctgttg gtttgtcatc ctgagtgcag cgaaggatct ttcagttaat tcgtcatcgc
     3421 gaggaacgaa gcgattaccc tgttggtttg tcatcctgag tgcagcgaag gatctttcag
     3481 tt
//
LOCUS       DAOQAD010000142         3245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_40267_length_3245_cov_6.203762, whole genome shotgun sequence.
ACCESSION   DAOQAD010000142 DAOQAD010000000
VERSION     DAOQAD010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40267_length_3245_cov_6.203762"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1126)
                     /gene="galE"
                     /locus_tag="PKL60_08420"
     CDS             complement(116..1126)
                     /gene="galE"
                     /locus_tag="PKL60_08420"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNW96196.1"
                     /translation="MKILITGGAGYIGSTIASVLEDNGHTPIILDSLVTGRREFTRGR
                     IFYEADIADRAMVRQVFSEHPDIFATIHCAALIVVPESVARPYDYYHDNVVKSMTFFK
                     LLSELGYPRVVFSSSAAIYDVVPGFMVTEESPLRPLSPYSRTKYMMEMILKDFSSAYN
                     LKAIALRYFNPIGADPQMRSGMHIKNPSHVLGKLVQVALGQDSVFYLTGVDWPTRDGS
                     GIRDYIHVWDLARAHLNAVERFDQVIAEASEEDSQYCVINLGTGKGVTVRELVAAFEQ
                     AYGQRVPVSIQPPRPGDVAGAYANADKAARLLGWKAELPITQGISDALKWGKVRENII
                     HW"
     gene            complement(1151..2485)
                     /locus_tag="PKL60_08425"
                     /pseudo
     CDS             complement(1151..2485)
                     /locus_tag="PKL60_08425"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          706 a          907 c          991 g          641 t
ORIGIN      
        1 gcacttgttg ggtttcgctc ctgcgtcgct gcacccaacc tacatgctgc acccatccta
       61 gcactacatg ctgcacccaa ccaacacctg cacccaacct acaaccgccg tctgcttacc
      121 agtgaatgat attctcccgc accttgcccc atttcagcgc gtctgagatg ccctgggtga
      181 tgggcagttc ggctttccaa ccgagcaggc gggcggcttt gtcggcgttg gcgtacgccc
      241 cggccacatc ccccgggcgc ggcggctgga tggaaaccgg tacgcgctgc ccgtaggctt
      301 gctcaaacgc ggcgaccaat tcccggacgg tgacgccctt gcccgtcccg aggttaatca
      361 cgcagtactg cgaatcttcc tcggaagcct cggcgatgac ctggtcaaaa cgctcaaccg
      421 cgttcaggtg cgcccgcgcc aggtcccaga catggatgta gtcgcggata cccgagccgt
      481 cgcgcgtagg ccaatccacg ccggtgaggt agaagacaga atcctgcccg agcgcgacct
      541 gcaccagctt gcccagcacg tgcgaggggt ttttgatgtg catgccgctg cgcatttgcg
      601 ggtccgcgcc gatggggttg aaatagcgca gcgcgatggc tttcaggtta taagcgctgg
      661 agaagtcctt aagaatcatt tccatcatgt acttggtgcg ggaatagggg ctgagcgggc
      721 gcagcgggga ttcctctgtg accataaagc cgggcaccac gtcgtaaatc gccgcggagg
      781 agctgaacac tacgcgcggg tagcccagtt cgctcaggag cttaaagaag gtcatcgatt
      841 tgactacgtt atcgtgatag tagtcatacg ggcgggcgac ggattccggc acgacgatga
      901 gcgcggcgca gtggatggtg gcaaaaatat ccgggtgctc cgagaaaacc tggcgcacca
      961 tggcgcggtc ggcgatatcc gcctcataaa agatgcgccc gcgcgtgaac tcgcgccgtc
     1021 cggtgaccag ggaatccaga atgatgggtg tgtggccgtt atcttccagg acagaagcaa
     1081 tcgtgctgcc gatatatcct gccccgccag tgattagaat tttcattact ctccttatct
     1141 atatcctgcg tcaatactgc atgggttggc tggctgtcac tttgcgcacc gttaacaata
     1201 ccaccatccc gaccaacagg aacagcagga tggacccgac tgcccagtat aagccggcct
     1261 gttcggccgc aaactcgctc aggcccctgg ccagatagag gttgtgcgcc cggtacgaga
     1321 ccgtgctgaa cacaaagggt ccgacgaagg tggaagtgcg cccggcgacg gagaggaagc
     1381 cgtagaattc ggtggttttg cccgccggcg ccagttggct gaccattgtg cggctgactg
     1441 cctgcgctcc ggagagggag aaacccgcca cggcgccgat gacgaagaac atcacggtgg
     1501 acttgatgaa gaacagggcg gtcaacgcgg caatcagaat caggagcgag atgagaatgg
     1561 cctgcttgct gctttgcttg tccgaaattc tgccaaacaa gagcgcgccg ccagccccgg
     1621 cggcctggat cacaatcacg aacaggatca actgcacctg ccccatcccg aagagcgtcg
     1681 ctccgataat cgcggcaaag tccatcagca tcatgatgcc gtcgttgtaa atcaggaaag
     1741 cgactatata cttcagaaac tcgctgtagt gtttgatttc gcggaaagtc ttccccaact
     1801 tctggaacgc cagcccgaat actgtttcac ccgcggggat gacttccggc tcggtaatct
     1861 cgcgcacgtg caagaaggtg gggatggagg aaagcaggaa gaacagcgcg gtaatcagga
     1921 aagtgtaggg gatgaagctg ttgccgataa gctgcatggg caccagcacg ataatcaggc
     1981 aggctacgcc gcccagcatg ccgaccgccc agccttttcc ggagatgtag ccgatcgtct
     2041 caggggtgga gacgtccacc agcagcgcgt cgtaaaagac ttgcgcgccg cggtagccaa
     2101 tttccgccag gatgaagaac agcatgccga tgaagatatc gcccgggcgc acgaaaaaca
     2161 gcaaagcggt gaagaccacg gagatgctgg tgaagatgaa gagcaggcgc ttcttggagc
     2221 gtgaaaaatc ggcgatgctg ccgagcaccg gggagatcag ggcaacgagc acggcggcga
     2281 tgccgaccga ccagccccac aggcgggtgc cttccgcgcc gccgaccacc gcgtttttga
     2341 agtaagcgga atagactgcc agcaggacaa tggccgcgta ggcggagttg ccgaagtcgt
     2401 agaagtacca ggcgtgccgc gctcggcggg cgggcttggt ctggtcgagc aggtttttgg
     2461 gaggtgtttc catgaaggtg tcctttgagt ctctttgatg atgctctgaa ttatcgcaag
     2521 cttgatttta tcagactccc cgcggagttt tcctttttct tccccgaggg gatgggcttg
     2581 tttgacaccg ctagacgcga agcggccttg cttctagttc gtcaccgcga ggaacgaagc
     2641 ggtctcccgg ccggcaagaa ggcggatccc acagggaatg cctggctctg ccgcaccggt
     2701 ttgccgccgc gaacccgcca gtccgttact gagagaccct ttgcctctga gcggcaaaag
     2761 ataaattcgc gattaccagg caaagggtcg cggcagtccg cctttaatgc tgttaatttg
     2821 ggcaccgcag cgggtaatca ataactcaga agtaggttgg gtggagcggc agcgcaaccc
     2881 aacaatattc acctggcgag cgcatgcaac ttgttgggct gcgttcctgt gctccagctg
     2941 cagccaggga ttcctggatg attgtcatcc tgagtttccg aaggatctat tctataacgt
     3001 gaaagtggta ggaagattct tcacttcgtt cagaatgacc agaggtaggt tgggtggagc
     3061 gccagcttag cccaacagca gatgggtagg ttgggtggag cggcagctta gcccaacagc
     3121 agatgggtag gttgggtgga gcggcagcgc aacccaacaa agtaatcagg ttggcgcgta
     3181 ggtgcagctt gggcagcgga ggtttggtat ttttgttggg tttcgctcct gcgtcgctgc
     3241 accca
//
LOCUS       DAOQAD010000143         2879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_48142_length_2879_cov_4.897663, whole genome shotgun sequence.
ACCESSION   DAOQAD010000143 DAOQAD010000000
VERSION     DAOQAD010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48142_length_2879_cov_4.897663"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(506..1078)
                     /locus_tag="PKL60_08430"
     CDS             complement(506..1078)
                     /locus_tag="PKL60_08430"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNW96197.1"
                     /translation="MAYPVANPKVIQRARQVLALKPLFLDTETTGTGPGDVIIEVGIV
                     DLDGNALYDKLVNPGMPIPPESSAVNEITDEMVANADPWPAVWAEIEPILRMRAIGIY
                     NADFDMRLIRQSNAAHGIRTGLADAQAFCVMKLFAAYYGEWNPRYNGFKSQKLEFAGR
                     VCQLDLANTHHAIDDARLTAALLRYIASRG"
     gene            1258..2007
                     /locus_tag="PKL60_08435"
     CDS             1258..2007
                     /locus_tag="PKL60_08435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNW96198.1"
                     /translation="MTQPYQRFARHYHEGHYTQFARWVSETVFPHFQEALSFQPASLL
                     DLACGSGIFAARMAAKGLRVTGLDLSAQMLEIAREQSSAVTWVQADMSRFKLAERFDC
                     VTCFFDSLNYLLKAEELARTFQAASAHLNPGGYFIFDMNTIYGLAVSWQRFPYNLNQE
                     SEDYLELMGTAYDYELDIADVRFIMFEQEGELWARYEEIHRERGYHLDDILLMLEQAG
                     LQPGYVLGDPLSLRPLAESDGRVWITAWKPG"
     gene            2310..>2879
                     /locus_tag="PKL60_08440"
     CDS             2310..>2879
                     /locus_tag="PKL60_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96199.1"
                     /translation="MVTLIVGAGFSKWAANLPVAKELFDFAVEPFGVNEENKLCSIRA
                     IKASWDEAHPTDTAEAFIDFAMHQNDRTSKLVVWYVVRRLSEPYTWKEWHAGKWRRHV
                     LMLDENRKYSRPGVDEAQSFLLGILGTHLQGVITTNYDLLIEYALGTKGFNYGVKGEA
                     LVGRGAYPVSTWSNPVDLTGRVSLAKVHGS"
BASE COUNT          662 a          796 c          797 g          624 t
ORIGIN      
        1 cgctgctggg agtaatggcg cgtcagaaat gaaagaaggg ggcgcggcga tctgcatgaa
       61 agcgaacagc aagatccttc gctgcggcct gcgcgcttca gggtatcatg atgactttgg
      121 aattacggca gggagatcac ttcgcaagac ctgtaccctc tagggtatcg cgatgacaaa
      181 atgacgaagc tcgccttgca cgcccgcgat gacgaggagc gctaagtttg taggttgggt
      241 gcagcgacgc aggagcgtaa cccaacatcc acaggcagac ctgcacgctt aagctgacga
      301 aaaacagtta tcattctgaa cgaggtaaag aatctatctt ttgaaaagcc aaatctatcg
      361 ctcgcgctca ggatgacaag ccgtataagc tgggtgcaga ggcgcggtag cgaaaccaaa
      421 cggcggctct acaaacccgc tggccaaagc ttgtcgggtt gcgctgccgt tccacccgac
      481 ctacaggcat cgcaatgagg cccgctcacc cgcgcgaagc gatataccgc agcagcgctg
      541 ccgtcaggcg ggcgtcgtca atcgcgtggt gcgtgttcgc caggtccaac tgacagaccc
      601 gccccgcgaa ctcaagcttc tgggacttga acccgttgta acgcggattc cattctccat
      661 aatacgccgc aaaaagcttc atcacgcaaa atgcctgcgc gtccgcgagg cctgtacgga
      721 tgccgtgcgc ggcgttggat tggcggatga gccgcatgtc aaaatccgcg ttataaatcc
      781 caattgccct catgcgcagg atcggctcaa tttccgccca aactgccggc caggggtccg
      841 cgttcgcgac catctcatcc gttatttcgt ttaccgcgct ggattccggt gggatgggca
      901 tacccgggtt gaccagtttg tcatacaagg cgtttccatc aaggtccaca atccctactt
      961 caataatcac atctcccgga ccagtaccgg tggtttcggt atccaaaaag agcggtttga
     1021 gtgccagaac ctgtctggcg cgctgaatga ctttcggatt ggcgacggga tatgccatat
     1081 tgcggtcctt tccctgcgga atttttcatt taatatacca ttacctgact ttgtgatgga
     1141 cttgcattga aaatcagcgc caagcctgtc cggcgcattc gcgcggcaga gccagccctt
     1201 cgccccgacc aaccctgatg ttataatttt cctattccca aaccccaaag gaaccagatg
     1261 acacagccgt accaacgctt tgcccggcac taccacgaag gtcactacac tcaattcgcc
     1321 cgttgggtaa gcgaaacggt attcccccat ttccaggaag cgctttcctt tcaacccgcc
     1381 tcgctattgg atttagcctg cggcagcggt atcttcgctg cgcgcatggc tgcgaagggc
     1441 ctgcgcgtca ccggcctgga cctctccgcg cagatgctgg aaatcgcgcg ggaacaaagc
     1501 tccgcggtca cctgggtgca agcggacatg agccggttca agctcgccga gcgttttgac
     1561 tgcgttacct gcttcttcga cagcctcaat tacctgttga aagccgagga acttgcgcgc
     1621 accttccaag ccgccagcgc gcacctgaac ccgggcggct attttatctt cgacatgaac
     1681 accatctacg gactggcggt ctcctggcag cgcttcccgt acaacctgaa ccaggaaagc
     1741 gaggactacc tggagctgat gggcaccgcg tatgattatg agttggacat cgcggacgtg
     1801 cgctttatca tgttcgagca ggaaggcgaa ctctgggcgc gctacgagga aatccaccgc
     1861 gaacgcggct atcacctgga tgacattctg ctcatgctgg aacaggctgg gctgcagccg
     1921 ggctacgtcc tgggcgaccc gctgagcctg cgcccgctgg ccgaaagcga cgggcgcgtg
     1981 tggattaccg cttggaaacc aggttaggcg aagatgcgca ggggattgga ccaggcagaa
     2041 aaagttaggc gcgactcaca aaacttcgtc tttctcggag gggcgggggc ctgatcacgc
     2101 cggtctcaga ccgcgcgcgg tattgtaccg ataggtcgga tggatcagaa tcgaaacagc
     2161 tcaacttttc aatggttgga tatatactaa aaaattcatc tcctctaatc agagggtttc
     2221 agataatcaa gcagaattgc aatctatcat ccttgattgg ccaggtaata cagtatagtt
     2281 agtagatgac ttgaagtgag gcgtagttga tggtcactct catagtcggc gctggtttct
     2341 ccaagtgggc cgcgaatctc cccgtggcga aggagctgtt cgactttgcg gtcgagccgt
     2401 tcggcgttaa tgaggaaaat aagctctgct ccataagagc aatcaaggcc tcgtgggatg
     2461 aagcacaccc taccgacaca gcagaagcct tcatcgactt cgccatgcat cagaacgacc
     2521 gaacttcgaa gttggtggtt tggtacgttg tccgccgtct atcagaaccc tacacttgga
     2581 aagaatggca cgcgggcaaa tggcgccggc atgttctgat gctcgatgag aatcgtaaat
     2641 acagcagacc aggcgtggac gaagcacaga gtttcctcct cggcatattg gggacgcatt
     2701 tgcagggtgt catcaccacc aactacgatt tgttgattga gtatgcattg ggcaccaagg
     2761 gatttaatta cggcgtgaaa ggggaagccc tggtcggacg cggtgcttat ccggtgtcca
     2821 cttggtcgaa tccagtcgat ttgaccggtc gtgtatctct ggcgaaagtt catggcagt
//
LOCUS       DAOQAD010000144         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_51594_length_2753_cov_6.376946, whole genome shotgun sequence.
ACCESSION   DAOQAD010000144 DAOQAD010000000
VERSION     DAOQAD010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51594_length_2753_cov_6.376946"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..565)
                     /locus_tag="PKL60_08445"
     CDS             complement(158..565)
                     /locus_tag="PKL60_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HNW96200.1"
                     /translation="MEVPRYWRLKKQRYALVGETCPHCNAKIFPPRDICPECGGEAKT
                     PFTFSGQGEVYSYTHMASAPAGFEEQAPYTIALVKLTEGPMVTAQLTDLGDEPVRIGM
                     PVEMVTRKIRNDGDEHGIIVYGYKFRPVMLGPH"
     gene            742..1962
                     /locus_tag="PKL60_08450"
     CDS             742..1962
                     /locus_tag="PKL60_08450"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNW96201.1"
                     /translation="MPELSLTLPQARALTLAAQGLHEAASRPAVKSDILNAIRRIHNL
                     QLDTISVVARAHLHILWSRLGAFEPRWLEELHMEGALFEFWAHAACLLPIEDYPLYRS
                     LMRQQFIGWENIRQWGEANPAVIADILAHLRANGAVKSSDFAREGPRGKWWDWKVEKV
                     ALEYLYYKGDVMIASRRGFQRVYDLRERVLPDWDDARIPPYQDALHQLVLMSVKALGA
                     ARENWVAPYFYLPKRPVAAVLAELQAAGEILPARVAGLEGKPVYVHPHNLELLEQACA
                     GSLQAKTTTILSPFDPLISDRARTQLLFGFAYQLECYLPAAKRKYGYFSLPILHNGSL
                     VGRMDAKAHRKEKRLEVIQLFLEEGVRPEDALLAELARTLRAYASWQGLSDVSVTASE
                     PARAAEALNTCLRG"
     gene            complement(1973..2524)
                     /locus_tag="PKL60_08455"
     CDS             complement(1973..2524)
                     /locus_tag="PKL60_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNW96202.1"
                     /translation="MHALISTLPKPYEDQVLQIWDDLEKRFGCKYVRTAAVPHFTWQL
                     GERYDLDAVIPVLKTAAEALEPFDIVVQGVQHFVSQSPVVFLEIIKNAALVKTHLKLW
                     QLLQEHTAEPNLLYSPALWHPHITLAVQDLSWEDLPKALAFLQSRNIDWRVMIDHFSI
                     LRQEPDGAVTLEHHFQFGRGKVY"
BASE COUNT          567 a          789 c          790 g          607 t
ORIGIN      
        1 ttaaataccg ttcctcaaac agatgaggaa cggcttgaac aatttgagtt gaaactaagg
       61 attgtaaata ctttggtcga gaaggtctgc atccacaagg attggtcttt agaggttacg
      121 atccgtttaa acctcctcca gataattcgg cagcggccta gtgcggacca agcatcaccg
      181 gccggaactt atacccgtac acgataatcc cgtgctcgtc gccgtcattc cttatcttgc
      241 gggttaccat ctccaccggc atgccaatcc gcaccggctc gtcccccagg tccgtcagtt
      301 gggcggttac catggggcct tccgtcagct tcaccagcgc gatggtatac ggcgcctgct
      361 cctcaaaacc tgccggcgcg ctggccatgt gggtgtagga atacacctcg ccctgaccgc
      421 tgaaagtgaa aggggttttc gcttctccgc cgcattccgg gcaaatatcc cggggcggga
      481 atattttcgc gttgcaatgc gggcaggtct cacctaccag cgcgtagcgt tgctttttca
      541 gtctccaata gcgtggaact tccattttgt actccttaca gcctttactg ttcacagagt
      601 ttaacgattg cagactctca tttgctatca ggtttcgcgc ttcccgctgg aatttctctc
      661 aacgttgcca atcagcacgc atagagcacg cagaaagccc ggtttccgaa cgctcccgat
      721 ttttcgcgta caattaccgc tatgcccgag ctaagcctca cactccccca ggcgcgcgct
      781 ctaacgctcg ccgcccaagg cctgcacgag gctgccagcc gcccggctgt caaatccgac
      841 atcctgaacg caatccggcg catccacaac ctgcagctcg acacaatcag cgtggtggcg
      901 cgcgcgcacc tgcacatcct ctggtcgcgc ctcggggcgt ttgaaccgcg ctggctggaa
      961 gagctgcaca tggagggcgc gctgtttgaa ttctgggcgc acgctgcctg cctcctcccg
     1021 attgaagact acccgctcta ccgcagcctg atgcgccagc aattcatcgg ctgggagaat
     1081 atccgccagt ggggagaagc aaaccccgct gtcattgccg atattctcgc tcatttgcgc
     1141 gcgaacggcg cggtgaaatc ctcagatttc gcgcgtgaag gaccgcgcgg caagtggtgg
     1201 gattggaaag tggaaaaggt cgcgctggaa tacctgtact acaagggtga tgtgatgatt
     1261 gccagccgcc ggggttttca gcgcgtgtac gacctgcgcg agcgcgtttt gccggattgg
     1321 gacgacgcgc gcattccccc ttaccaggac gcgctgcacc agctggtatt gatgagcgtg
     1381 aaagctttgg gcgccgcccg cgagaattgg gtcgcgccat acttttacct gcccaagcgt
     1441 ccggtggccg ctgtcctcgc ggagctgcag gcagcgggcg agatcttgcc ggcgcgggtg
     1501 gcgggcttgg agggcaaacc ggtgtatgtg cacccgcaca acctggaact gctcgaacag
     1561 gcttgcgctg gctccttgca agcgaagaca accaccatcc tttctccttt tgaccccctg
     1621 atctcggacc gcgcgcgcac acaattgctt ttcggctttg cctaccaact ggaatgttac
     1681 ctgccggctg ccaagcgcaa gtacggctac tttagtctgc ctatcctgca caacggcagc
     1741 ctggtcggcc gcatggatgc caaagcgcac cgcaaagaaa aaaggctgga agtgattcaa
     1801 ctgtttttgg aagagggggt gcgccccgag gacgcgctgc tggcggagct ggcaagaacg
     1861 ctgcgtgcgt acgcaagctg gcagggctta tccgatgtga gcgtgactgc ctctgaaccc
     1921 gcgcgggccg ctgaagcgct caacacttgc ctgaggggtt gaaccgcgtg ctctaatata
     1981 ccttgccccg gccgaattga aagtgatgtt cgagggttac tgctccgtct ggttcctgcc
     2041 gtagaatcga gaaatggtcg atcatcacgc gccaatcgat gttcctggac tggaggaaag
     2101 ccagcgcttt aggcagatcc tcccaactca ggtcctgtac ggccaacgtg atgtgcggat
     2161 gccagagcgc cgggctgtag agcaggttcg gttccgcggt gtgttcctgc aggagctgcc
     2221 agagtttcag gtgtgttttt accagggcag cgttcttgat aatttccaga aagacaaccg
     2281 gggattggct gacgaaatgt tgtacgccct gcacgacaat atcgaaaggc tcaagcgctt
     2341 ccgcggcggt ttttagcacg gggatgaccg cgtccaggtc gtagcgctca cctaattgcc
     2401 aggtgaagtg cggcacagcc gccgtccgca cgtacttaca gccgaagcgt ttttccaggt
     2461 cgtcccaaat ctgcaggacc tggtcttcgt aaggtttcgg gagtgtagag ataagcgcgt
     2521 gcatggggcc gtcctttctt tatgggagga ttatatcaag tgagggtggt aacgatgaat
     2581 cgtcattctg gatgaaggga agaatcgcaa agatagatcc ttccctgctc tcaggatgac
     2641 aaaacctctg tcattctgaa ggaagtgaag aatctctcgc gtgggagaac cagatccttc
     2701 gctggcgctc aggatgacga gctgtttaag ctgggtgcaa aagcgcagga gcg
//
LOCUS       DAOQAD010000145         2730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_52211_length_2730_cov_5.191028, whole genome shotgun sequence.
ACCESSION   DAOQAD010000145 DAOQAD010000000
VERSION     DAOQAD010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52211_length_2730_cov_5.191028"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PKL60_08460"
     CDS             <1..785
                     /locus_tag="PKL60_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNW96203.1"
                     /translation="LQIHTVPLNIHWDGTNYIDLVDITPAEFYKKLASSKTLPTTSQP
                     NAEAFVQVYEQLLNQDYDILTLPISSKLSGTYYSAMMAAARFPLGRVEVIDTLFTSCP
                     LSAIVIKTARAAQKGASLLECSNLARDAARRTKVYFAVETLEYLHKGGRINTASAFLG
                     SALNLKPILSFEDGLIVPVERVRTSRKAHERLLELAEQTVGPNGKIEFLGLVSAAAPQ
                     ALEDLRQKAHKRFQIEEELAMDLSPVLGCHVGNGTVGISLMA"
     gene            835..2160
                     /locus_tag="PKL60_08465"
     CDS             835..2160
                     /locus_tag="PKL60_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNW96204.1"
                     /translation="MDFSEKTGIQVPKVLLPDKTVDHTRWAVIACDQFTSQPEYWEKV
                     QEFVGDAPSTLRMILPEVWLHSPDVQARIQATQETMRAYLRAGILTEHEGFVLVEREH
                     SGQIQTGLMVALDLETYDYNKGSQTLIRATEGTILDRLPPRMQVRRGAPLELPHILVL
                     YDDPGFKVLEAVLKAKTRLPLCYDFDLMFGSGHLQGWQISEPELIRSVLSGLSALIEP
                     GAYADKYQLPEGTPPLLFAMGDGNHSLATAKAIWEELKPSVGMDHPARYALVEIVNLH
                     DPAINFEAIHRVLFNAPPDFLQQMESAFSPDIRFRRGLSFDQLSEAVLTRDPQSQKIG
                     CIRAADLVLIELTRPSYNLPVGSLQEYLDRFLASHPETRIDYVHGDDTVRELAAQPGN
                     IGFLLPPISKEAFFKTVIVDGALPRKTFSMGHAKDKRFYMECRRISRES"
     gene            2222..>2730
                     /locus_tag="PKL60_08470"
     CDS             2222..>2730
                     /locus_tag="PKL60_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNW96205.1"
                     /translation="MPAKKTSSSQPNLSADQNMNDAKKAVLDKALNDIIKRYGEGSIM
                     RLGEAHQMQVESIPTGALSLDLALGVGGIPKARITEIFGPESSGKTTLCQHIVAECQQ
                     RGGTAAFIDMEHALDPVYAARCGVNIDQLLISQPDTGEQALEIAETLVRSGAVELVVV
                     DSVAALVPRS"
BASE COUNT          627 a          822 c          717 g          564 t
ORIGIN      
        1 ctttacagat tcataccgtc ccgctcaaca ttcattggga cggcaccaat tatatagatt
       61 tggtggatat tacgccggcc gaattttaca aaaaactggc ttcttcaaaa actttgccca
      121 cgacctcaca gcccaacgct gaagctttcg tgcaagtcta tgagcagctg ctgaaccagg
      181 attatgacat cctgacactg ccgatttcct ccaaattatc aggaacttat tactcggcta
      241 tgatggcagc cgcccggttt ccactgggaa gggtggaagt gattgatacg ctttttactt
      301 cctgcccgct gagcgcgatt gtaataaaaa cagcccgggc agcccaaaag ggcgcttccc
      361 tgctggaatg ctccaatctt gccagggatg cggcccgcag aaccaaggtc tacttcgcag
      421 tcgagacgct tgaatacctg cataaaggcg gacgcatcaa cactgcctcg gctttccttg
      481 ggtccgcgct caatctcaaa ccaattctaa gctttgaaga cgggttgatc gtcccggtgg
      541 aacgcgtcag aacttcccgg aaagctcacg agcgcctcct ggaactggcg gaacagaccg
      601 tagggccgaa cggcaaaatc gaattcctcg gcttggtctc agcagccgcg ccgcaagcgc
      661 tggaagacct gcgccagaaa gcccataagc gctttcagat tgaagaagaa ctggcaatgg
      721 acttgagccc tgtgcttggc tgccatgtgg gcaatggcac tgtcggcatt tctttgatgg
      781 cttgatacca ttctctgata caattggggt atctcttcta tatagaggtt cccaatggat
      841 ttttcagaga aaactggcat ccaagtccca aaagtcctgc tgcctgataa gacggtcgac
      901 cacacccgat gggcggtaat cgcctgcgat cagtttacct cccagccgga atactgggaa
      961 aaggtccagg aattcgtcgg cgacgcgcct tccaccctgc gcatgatcct ccccgaggta
     1021 tggctgcaca gcccggacgt gcaggctcgg attcaggcaa cccaggaaac catgcgcgcc
     1081 tacctgcggg ccggtatcct cacagaacac gaaggatttg tgctggtgga acgcgaacat
     1141 tccggacaaa tccaaaccgg attgatggtc gcgctggacc tggagactta cgactataac
     1201 aagggctccc agacgctcat cagagcgacc gaaggcacta ttctggacag gctgcctccg
     1261 cgcatgcaag tgcgccgcgg ggcgccgctc gaactgcccc acattctggt tttgtacgac
     1321 gacccggggt ttaaagtcct tgaagcagtc cttaaagcga aaacacggct gccgctctgc
     1381 tatgattttg atctgatgtt cggcagcggc cacctgcaag gctggcagat ttccgaaccc
     1441 gaattgatca gaagcgtgct ctcgggtctt tccgcgttga ttgaaccagg cgcgtacgcc
     1501 gacaaatacc agctccctga aggcacgccg ccgctgttgt tcgcgatggg cgacggcaat
     1561 cactcgctcg ccaccgcgaa agccatctgg gaagaactca aaccctcggt tggaatggac
     1621 caccctgcgc ggtatgcgct cgtcgaaatc gtgaacctgc acgatccggc gattaacttc
     1681 gaagccattc accgcgttct gttcaacgcg ccgccagact tcctgcagca gatggaaagc
     1741 gctttctccc ccgacatccg gtttcgccgc ggccttagtt ttgaccagct tagcgaagcg
     1801 gtgttgaccc gggatccaca aagtcaaaaa atcggctgca tccgcgctgc ggacctggtg
     1861 ttaatcgaac ttacccgccc gagctacaac ctgcccgtcg gcagcctgca ggaatacctg
     1921 gaccgcttcc tcgccagtca ccccgaaacc aggattgatt acgtccacgg cgatgacacc
     1981 gtgcgcgagt tggcagccca acccggcaat atcggtttcc tcctgccgcc catttcaaaa
     2041 gaggcgttct ttaagaccgt catcgtggac ggggcgctgc cgcgcaagac cttctcaatg
     2101 ggtcacgcca aggacaaacg cttctatatg gaatgccggc gcatcagccg cgagtcttga
     2161 cacatcgaac aattgtgcta tattaaaggt agttatcccc ataagtattc ctggagacat
     2221 tatgcccgcc aaaaagacca gctcatccca acccaacctg tccgcagacc agaatatgaa
     2281 cgacgccaaa aaagccgttc tggataaagc tctgaacgat attatcaaac gctacggtga
     2341 aggcagcatc atgcgcctgg gcgaggcgca ccagatgcag gtggaatcca tcccgaccgg
     2401 cgcgctctcc ctggacctgg cgctgggcgt cggcggcatt cccaaagccc gcattaccga
     2461 aatctttggg cctgagtctt ccggtaagac gaccctctgc cagcacattg tggcggagtg
     2521 ccagcagcgc ggaggcacag cggctttcat cgacatggaa catgctctgg acccggtcta
     2581 cgcagcccgc tgcggcgtca atattgacca gctgctcatc tcccaaccgg ataccggcga
     2641 acaggcgctc gaaatcgccg aaacgctggt gcgctccggc gcggtggaat tggtcgtggt
     2701 ggactcggtg gccgcgcttg tgccccgctc
//
LOCUS       DAOQAD010000146         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_53024_length_2702_cov_5.327163, whole genome shotgun sequence.
ACCESSION   DAOQAD010000146 DAOQAD010000000
VERSION     DAOQAD010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53024_length_2702_cov_5.327163"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..782)
                     /locus_tag="PKL60_08475"
     CDS             complement(216..782)
                     /locus_tag="PKL60_08475"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNW96206.1"
                     /translation="MKSHEILSRETVLKAHAFDVVRLGCRLPDGRERAYDLVDHANAV
                     TLLPVTAQGEALLVTQYRIGAEGMMLELPAGVMDPGEQPLESARRELREETGMDCGEL
                     APLGSFFMVAGYSNECMHAYLALDLREAPLEQDEDEFLELERLPLAELYRRAFAGQLT
                     DGKTMVTLMLALPKLVGRFPGLLAGLGG"
     gene            complement(964..1620)
                     /locus_tag="PKL60_08480"
     CDS             complement(964..1620)
                     /locus_tag="PKL60_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNW96207.1"
                     /translation="MSKKYRILTAVLLLLLGLLSACQRPYSNAPVATPQVIDSASTPL
                     PVDQQILNATMTAQAIMEKFNQPTVMVTNAQGTPVVATPAGILLPTNTPVPNPTATPL
                     PPTPALTRPLTHTVQSGETIYCLARRYDVDPAEMLALNNYNSWLNVGDVLDVPQSGSW
                     PTEDRAQLPHPDTWTVSPGETVYSIACEYGDVWPEAIIAVNGLTAPYELQPGQVLQIP
                     "
     gene            complement(1758..1907)
                     /locus_tag="PKL60_08485"
     CDS             complement(1758..1907)
                     /locus_tag="PKL60_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96208.1"
                     /translation="MKKVVRASGKATELYYFSTDLRKGESVEAVPAGKVVIELPSGLP
                     VLKKK"
     gene            2087..>2702
                     /locus_tag="PKL60_08490"
     CDS             2087..>2702
                     /locus_tag="PKL60_08490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNW96209.1"
                     /translation="MNTLYYGDNLEVLQRHIRDESVDLIYLDPPFNSNASYNVLFAQK
                     DGKQSSAQIQAFEDTWQWDETAIQTYTREVEKGGPIADALRAFNLILGDSNMMAYLTM
                     MAPRLKELHRVLKPTGSLYLHCDPTASHYLKVLLDMVFGAENFRNEIIWVRSNPKSHG
                     TVNFPNCTDTILRYTKTNKYFFKQPYGQHNPEYVKSAYKYKDEKG"
BASE COUNT          549 a          813 c          803 g          537 t
ORIGIN      
        1 caggcgatct gggcttcccg ggggaaatct ggtttccaaa gctcctccaa agctcgcgcc
       61 gctctcctga ccgcgtggaa gccggcccaa aactcgcgcc gctctcctga ccgcgcccgg
      121 aggttgacct ttaggtctcc agtgtttttt taccgacgat taaaactctc gtccgctctg
      181 cgggtattgc ccctcatcgg aaagtcaaat atcccctacc cgcccagccc cgccagcagc
      241 cccgggaagc gcccgaccag cttcggcagc gccagcatca gcgtcaccat cgtcttcccg
      301 tccgtcagct ggccggcaaa cgcgcgccgg tagagctccg cgagcggcag gcgctccagc
      361 tccaaaaatt catcctcgtc ctgttccagc ggcgcttcgc gcaggtccag cgccaggtag
      421 gcgtgcatgc actcattcga atagcccgcc accatgaaaa agctgcccaa cggtgccagc
      481 tccccgcagt ccatgccggt ttcttccctc agctcccggc gcgcgctctc caaaggctgc
      541 tcgccagggt ccatcacgcc cgccggcagc tccaacatca tcccctccgc cccgatgcgg
      601 tactgcgtca ccagcagcgc ctccccctgc gccgtcacgg gcagcagggt caccgcgttg
      661 gcgtggtcca ccaggtcgta cgcccgttcg cgtccatccg gcaagcggca gccgagcctc
      721 accacgtcaa aggcgtgcgc tttcaaaaca gtttcgcggg agagaatttc atgggatttc
      781 atgctgtcct ttcggttgtt atgggccggc gtctttggag agccagattt ggttgttctc
      841 cgctgatttt acccgaaagc cccccgcccg cgcgccggca agcctgccgg tcccgctgca
      901 cacccgggca aacaaaaacc ggagggcata cccgccctcc ggcatctcaa actcaaaaca
      961 cgcttacgga atttgcagca cctgcccggg ctgcagctca tagggtgcgg tcagcccgtt
     1021 cacggcgata atcgcctcgg gccacacgtc gccgtactcg caggcgatgc tgtagacagt
     1081 ctcgcccgga gaaacggtcc aggtatccgg gtggggcagc tgcgcgcggt cttcggtcgg
     1141 ccagctcccg ctctgcggca catccagcac gtcccccacg ttcagccagc tgttgtaatt
     1201 gttcagcgcc agcatctcgg ccgggtccac gtcgtagcgg cgcgccaggc agtaaatcgt
     1261 ctcgccggac tgcacggtgt gggtcagcgg gcgggtcagc gcgggcgtgg gcggcagggg
     1321 cgtggcggtc gggttgggca ctggcgtgtt ggtcggcagc aaaatgcccg ctggcgtggc
     1381 caccacgggc gtgccctgcg cgttggtgac catcacggtc ggctggttga atttttccat
     1441 aatcgcctgg gcggtcatcg tggcgttcag gatttgctgg tccacgggca gcggcgtgct
     1501 ggccgagtca attacctggg gggtggccac cggcgcattg gaataggggc gctggcaggc
     1561 agagagcagc ccgagcagca gcaagagcac ggcggtaagg atgcggtatt tctttgacat
     1621 gggtcatctc caatcatgcg cgaaaatctt acagcttagg atgcaagctt cgtgccaggg
     1681 tagagagaaa gaaaaccgac aggggcggcc tgccggttca aaagggcgtt gagcggaacg
     1741 cgccgcccgg gcgggaacta cttcttcttc aggacgggca gccctgaggg cagctcgatg
     1801 acgactttgc cggccgggac ggcttccacg ctttcgcctt tgcgcaggtc agtggagaag
     1861 tagtacagct cggtcgcttt cccactggcg cggaccactt tcttcatatg cagaaagtat
     1921 tccttaccct ttgagttttt gaagctgaat gccatgttaa ctcctttgtc aattggcttg
     1981 atgaatctag tttacagagg tttcgggcga atgtcaaccg gaagtagagg ggcggggaga
     2041 gggtataatt ccggatagga cgcgcgggag caggcgaggt ggagcgatga atacgctgta
     2101 ctacggggat aatctggaag ttttacaacg gcacatccgg gatgaatcgg tggacttgat
     2161 ttacctggac ccgccgttca attcgaatgc cagttacaac gtgctgttcg cgcagaagga
     2221 cggcaagcag tccagcgcgc aaatccaggc gtttgaggat acctggcagt gggacgagac
     2281 cgccatccag acctacacgc gcgaggtgga aaagggcggc ccgatcgcgg acgcgctgcg
     2341 ggcgttcaac ctgattttgg gcgacagcaa catgatggcg tacctgacca tgatggcccc
     2401 gaggttgaaa gaactgcacc gggtcctcaa gccgaccggc agcctgtatt tgcactgcga
     2461 cccgaccgcc agccactacc tgaaggtgct gctggatatg gtctttgggg cggagaattt
     2521 tcgaaatgag attatatggg ttcgctctaa tccaaaaagt catgggaccg tgaattttcc
     2581 aaattgcaca gacacaatat tgagatatac aaagaccaat aaatactttt ttaaacaacc
     2641 ttatggacag cataatcctg aatatgtcaa atcagcttat aaatacaaag atgaaaaagg
     2701 tg
//
LOCUS       DAOQAD010000147         2633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_55213_length_2633_cov_6.729635, whole genome shotgun sequence.
ACCESSION   DAOQAD010000147 DAOQAD010000000
VERSION     DAOQAD010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55213_length_2633_cov_6.729635"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..394)
                     /locus_tag="PKL60_08495"
     CDS             complement(164..394)
                     /locus_tag="PKL60_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96210.1"
                     /translation="MRKNQIRFEYQDKPVICPVCQSTRIAEILWGMPAFSEQLMEDLD
                     AGKVALGGCCVSEDDPVWRCADCRKDFYRKSS"
     gene            complement(471..674)
                     /locus_tag="PKL60_08500"
     CDS             complement(471..674)
                     /locus_tag="PKL60_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HNW96211.1"
                     /translation="MLPEISLGDRVRLRKAHPCGGFEWEVVRLGADIGLVCLTCGHRV
                     LLTRRELARRMKARLPKEEKDEG"
     gene            717..1358
                     /locus_tag="PKL60_08505"
     CDS             717..1358
                     /locus_tag="PKL60_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNW96212.1"
                     /translation="MLNLSTPELISRLVTLVIALTLHEYAHARAAVFFGDDTPRLAGR
                     LTLNPLRHLDPLGSLMLVVAGFGWARPVPINPEQLRQRSPSALMLVSLAGPLSNMLLA
                     GLGGLVFLLLKLYAGTSLVRDTFFGKFLISFVLLNVTLCIFNLLPVAPLDGEKVLEYL
                     LPEPARKVLYAIRPYGMFILLALVFVLPMMGMDVFGTLIYAPVMAISRFLLGL"
     gene            1370..1711
                     /locus_tag="PKL60_08510"
     CDS             1370..1711
                     /locus_tag="PKL60_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNW96213.1"
                     /translation="MAYYCYLVECANGAYYAGWTLDPLRRVRQHNAGKGARYTRQNAP
                     VRLVYVELAPDRVSAMKRELQIKRLGHEGKQKLANRAEGNLAGEFLAAEREAEQEQAK
                     PAGRNQEGSGA"
     gene            1776..2372
                     /locus_tag="PKL60_08515"
     CDS             1776..2372
                     /locus_tag="PKL60_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNW96214.1"
                     /translation="MEAQRSVTKVRYKNTNCYFVSAQGGLLAFDAGWPGTYRAYKDGL
                     REQGWSVKDVRWLLVSHFHLDHAGLAGILVENGVEFVVFPEQVAAVAEMESLIARKAI
                     PYQAIDAAKIRRLELADSRAWLARIGIQGEVLHTNGHAEHSVSLLLDSGEAFVGDLTP
                     ESMVAEDDLLSQASWALLRAKGAHRIYPAHAQEFVLPG"
BASE COUNT          516 a          755 c          819 g          543 t
ORIGIN      
        1 gtcaacagcc ttgcatggtc gggggaccat gcaagaacgt cgatggtcat tctgagcgaa
       61 gtgaagaatc taatgagtcg gaaggataga ttcttcgctg atgcttagaa tgacacgacc
      121 aagattctgt gagtaatagc aacgtatggt atgaacagat tgctcaagac gactttcggt
      181 agaaatcttt acgacaatca gcacagcgcc aaacagggtc gtcctcactg acacagcagc
      241 cgcccaaagc gactttaccc gcgtccaaat cttccatcag ctgctcagaa aaggcaggca
      301 tcccccacag gatttccgcg atgcgcgtgg actggcacac cgggcagatg acaggtttat
      361 cctggtattc gaatcgaatt tgattttttc tcacgttcgt tcaagcccct tttcttttat
      421 ttttgtggaa agacaatgct gacctaggct tatcctatca tccccccccc tcacccctcg
      481 tccttctcct cctttggcag gcgcgctttc atcctcctgg caagctcgcg gcgggtcagt
      541 aatacgcggt gcccgcaggt gaggcacacc aggccgatat ccgcgcccaa acgcaccact
      601 tcccactcaa aacccccgca agggtgggct ttgcgcaggc gcacgcggtc cccaagcgat
      661 atttccggca gcatgagcct attatatccg catgatataa tcccggcaga aacgccatgc
      721 tgaacctctc gacgcccgaa ctgatttccc gcctggtgac cctggtcatc gcgctcacgc
      781 tgcacgagta cgcgcacgcc cgcgccgccg tgttcttcgg ggatgacacc ccgcgcctgg
      841 ccggccggct gaccctcaac cccctgcggc acctggaccc gctcggctcg ctgatgctgg
      901 ttgtggcggg cttcggctgg gccaggcccg tgcccatcaa cccggaacaa ctcaggcagc
      961 gcagtccttc ggcattgatg ctggtctcgc tggcgggtcc gctctcgaac atgctcctgg
     1021 cggggctggg cggcctggtg tttctgctgt tgaagttgta cgcaggcact tccctggtca
     1081 gggatacctt tttcggtaaa tttctaatca gcttcgtgct gctgaacgtc accctgtgca
     1141 tcttcaatct gctgccggtt gccccgctgg acggcgagaa ggtgctggaa taccttctgc
     1201 cggagcccgc gcgcaaggtg ctctacgcca ttcgcccgta cgggatgttc atcctgctgg
     1261 cgctggtgtt cgttctgccg atgatgggga tggacgtgtt cggcacgctc atctacgcgc
     1321 cagtgatggc gatttcgcgc tttttgctcg gattgtgagc aggttcccag tggcatatta
     1381 ctgctatctg gtggagtgcg caaacggcgc ctactacgcc ggctggacct tggacccgct
     1441 gaggcgcgtg cggcagcaca atgccggcaa gggcgcgcgc tatacccgcc agaacgcccc
     1501 ggtgcggctg gtatatgtgg agctggcgcc ggatagggtt tctgccatga agcgcgagct
     1561 gcaaatcaaa cggctgggtc atgaaggcaa gcaaaagctg gcgaaccgcg cggagggcaa
     1621 cctggcgggg gaattcctgg cggcagagcg ggaagcggaa caggagcaag cgaagccggc
     1681 agggagaaac caggaagggt ccggggctta ggcggtcggg cgggcagaca ggattgcttc
     1741 accgcagcct gtatggaaat gggtgggata cgcgcatgga agcacaaagg tctgtaacca
     1801 aggtacgata caagaacaca aactgttact tcgtgtcggc gcaggggggc ttgctggcgt
     1861 ttgacgccgg ctggcccggc acctaccgcg cgtacaagga cgggctgcgg gaacagggct
     1921 ggtctgtaaa agacgtccgc tggctgttgg tgagccactt tcatttggac cacgccgggc
     1981 tggctgggat tctggtggag aacggggtgg agttcgtcgt tttcccggaa caggttgcgg
     2041 cagtcgcgga gatggagagt ttgatcgcgc gcaaagccat accctaccag gcgattgacg
     2101 ccgcaaaaat tcgccggctg gagcttgcgg attcgcgcgc gtggctggcg cggattggca
     2161 ttcaggggga agtgctgcac accaacgggc acgcggagca cagcgtttcg ctgctgctgg
     2221 acagcggcga ggcgttcgtg ggggacctga cgcctgagag catggtggcg gaggatgacc
     2281 tgttgagcca ggccagttgg gcgctgctgc gcgcgaaggg cgcgcacagg atttaccctg
     2341 cccacgcgca ggagtttgtc ttgccagggt gatgaaagac ggcgctgttg gcgaaagctg
     2401 ccagaccgag ccgggatgag gccgatggcg ctttgactga cctccggggg tgtaggttgg
     2461 gtgcagcgac gcaggagcga aacccaacga caaactccaa tcactaaacc cgctggccgt
     2521 ttctcaccat ctgtggaatg ttgggttgcg ctccgattgt atcttttcga aactacatcc
     2581 ggtgtcgctc cacccaacct actacttcac caagcgaatt gtgttgggtt gcg
//
LOCUS       DAOQAD010000148         2541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_58298_length_2541_cov_7.290829, whole genome shotgun sequence.
ACCESSION   DAOQAD010000148 DAOQAD010000000
VERSION     DAOQAD010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58298_length_2541_cov_7.290829"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..1535)
                     /locus_tag="PKL60_08520"
     CDS             complement(327..1535)
                     /locus_tag="PKL60_08520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNW96215.1"
                     /translation="MIQARVKKLVKTRPVALIVLIFLAFIALGMPDGLLGVGWPSIRA
                     GFGQPLDAIGLLLFTMMVGYLSSSFLSGELTRRWGVGKVLIISCLVTGAGLIGYTLVP
                     RWWMMAALGVFAGLGAGAVDSSLNAYVAANFGPGLMQWLHASYGIGVTTGPLIMTAML
                     ARSGSWRPGYLVVGGFQLLLALSFALSLPLWNWKRADNAGEVEKAPAESASLKETLRQ
                     PRVWASMALFFFYVGSEVTLGTWVYSLLTEGRGIDPKLAGYFAGSYWFTFTLGRVLAG
                     VLTRKLKIQKLVVICIVAALAGTLVLGLNLGTWVNLAAVALVGFAFAPVFPGLMSGTP
                     ARVGRTHSNNTIGMQAAAGSLGGTGLTSLVGVLAQSFSLEVVPLVLLVFLSCLLGLYL
                     LFQRRESLSS"
     gene            complement(1551..2399)
                     /locus_tag="PKL60_08525"
     CDS             complement(1551..2399)
                     /locus_tag="PKL60_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNW96216.1"
                     /translation="MIKIITDSTCDIPPELLEKHDITVVPAYVLWNNEQFLDRVTLTA
                     EQFYARIESDPLRPTTSQPTSADFSRAFATAVENGAEEIICVTVSSAMSGTYQSAQNA
                     AKSAAVPVHVVDSKGPTMMVGWQALAAARALEAGKSAQEAVAAADQVRQNLVLVVGME
                     TLTYLSKGGRIGDAIFWLGNTLSVKPLVFINRQSGKVMPIALHRTHKGLVEGLYHKFQ
                     TSLSSLKNLHVAVLHGNSPEEAQKLAQRIREDLSPLELIVQMTGPVLGINTGPGALAL
                     CGYAES"
BASE COUNT          524 a          855 c          661 g          501 t
ORIGIN      
        1 cgctcctaaa tgtaaattgt tgatgaaggc ccgatgtcgc tccacccaac ctacgacctc
       61 gttctgtcgg gttgcgctga cgctccactc aacctacgcc tatgttctgt cgggttgcgc
      121 tgccgctcca cccaacctac gtctatattc ttttgggttg cgctgccgct ccacccgatc
      181 tacgcttacg ttctgtcggg ttgcgctgcc gctccgcccg atctacgctt acgttctgtc
      241 gggttgcgct gccgctccac ccgacctacg cctatgttct gtcgggttgc gctgctgctc
      301 cacctgacct gcgccttcgc tcgcgctcag gaggacaacg actcccggcg ctggaacagc
      361 aggtacagcc ccaacaagca gctcagaaat accagcagca ccagcggcac cacctccagg
      421 ctgaagctct gcgccaaaac gcccaccaaa ctggtcagcc ccgtgccccc caggctgccg
      481 gccgcggctt gcatgccaat cgtgttattg ctgtgcgtcc gccccacgcg cgccggcgtc
      541 ccggacatta agcccggaaa aaccggcgcg aaggcaaacc ctaccagcgc caccgccgcc
      601 agattcaccc aggtgcccag gttcagccct aataccagcg tgccagccaa cgcggcaaca
      661 atacagatga ccaccagctt ttggattttt aacttgcgcg tgagcacccc ggccaacacc
      721 cgcccaagcg tgaaggtgaa ccaatagctg ccggcaaagt agcccgccag cttcgggtct
      781 atgccgcggc cctcggtcag cagcgaataa acccaggttc ccagcgtaac ttcggagccc
      841 acgtagaaaa agaacagcgc catgctcgcc cacacgcgcg gctgccgtag ggtctccttc
      901 aggctggcac tttccgcagg cgctttttcg acctctccgg cgttatccgc tcgcttccag
      961 ttccagagcg gcaggctcag cgcaaaacta agcgccagca gcagttgaaa gccgcccacc
     1021 accaggtatc ccgggcgcca actgccggaa cgcgcgagca tcgcggtcat gatgagcggg
     1081 ccggtggtaa ccccgatgcc gtagctggcg tgcagccact gcatcagccc cggcccaaaa
     1141 ttcgcggcga cgtacgcgtt caggctggaa tccaccgcgc cggcgcccag cccggcgaac
     1201 acacctaagg ccgccatcat ccaccagcgc ggtaccagtg tgtaaccgat aagtcccgcg
     1261 ccagtaacca ggcagctgat aatcagcacc tttcccacgc cccagcgccg ggtcagttcg
     1321 ccgctcaaaa agctggagct cagatacccc accatcatgg tgaagagaag caaaccaatg
     1381 gcgtccaacg gctgaccaaa cccagcccgg atggaaggcc agccaacccc caacaaaccg
     1441 tccggcatcc cgagcgcgat gaacgccagg aagatcaata caatcagagc gaccgggcgg
     1501 gtcttgacca gtttttttac gcgggcttga atcatgccat tcctgtctgc ttagctttca
     1561 gcgtatccgc aaagcgccag tgcgcccggg ccggtattga tgcccaacac cgggccggtc
     1621 atctggacaa tcaattccag cggtgaaagg tcttcgcgga tgcgttgggc cagtttttgt
     1681 gcttcttccg gggagtttcc atgcaaaaca gccacatgca ggttttttaa actgctcagg
     1741 ctggtctgga atttatggta caagccctcc accaggcctt tatgggtgcg gtgcagcgcg
     1801 atgggcatca ccttcccgct ttggcggttg atgaacacca ggggctttac cgagagagtg
     1861 ttccccagcc aaaatatcgc gtccccaatg cgcccgcctt tgctaaggta agtcaacgtt
     1921 tccattccta ccacaaggac gagattctgg cggacctgat cggcagcagc cactgcctct
     1981 tgcgcgcttt ttccagcttc caatgcgcgc gccgccgcca gcgcctgcca gcccaccatc
     2041 atcgtgggac ccttggagtc gaccacgtgc acaggtaccg ctgccgattt cgcggcattt
     2101 tgcgcggatt ggtaagtacc gctcatcgcg ctgctgacgg taacgcaaat aatttcctca
     2161 gccccatttt ccaccgcggt cgcgaacgcc cggctgaaat ccgcgctggt gggttgggat
     2221 gttgtcggac gcagcggatc actttcaatc cgggcgtaaa actgctccgc agtgagcgtc
     2281 acgcggtcca gaaattgctc gttgttccat aaaacataag cgggaaccac cgtgatgtca
     2341 tgtttttcca gcagctccgg aggaatatca caggtgctgt ccgtaatgat ttttatcatc
     2401 gctcttctcc atcattttaa tagagcaatc ttaccccaga acccgcgaat caagcggatt
     2461 aagcaagggc tgccccgccc gcaggcagcc ggaagcatcg ctcattgccg ttatgcgccc
     2521 ccgatatgta gccgtttcgt a
//
LOCUS       DAOQAD010000149         2534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1271
            NODE_58536_length_2534_cov_4.633320, whole genome shotgun sequence.
ACCESSION   DAOQAD010000149 DAOQAD010000000
VERSION     DAOQAD010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854476
            Sequence Read Archive: SRR4236661
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:14:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,709
            CDSs (total)                      :: 1,677
            Genes (coding)                    :: 1,672
            CDSs (with protein)               :: 1,672
            Genes (RNA)                       :: 32
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58536_length_2534_cov_4.633320"
                     /isolate="AMR_MDS_1271"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PKL60_08530"
     CDS             complement(<1..686)
                     /locus_tag="PKL60_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNW96217.1"
                     /translation="MIASDVKLVYQRPLGFEERGTHYCPGCTHGVAHRLIAEVLEEMG
                     ELGHTIGVAPVGCAVFAYNYFAFDFVEAPHGRAPAMATGIKRSLPDRMVFTYQGDGDL
                     ASIGMGEIVAAAARGEQITTIFINNTNYGMTGGQMAPTTLPGQVTTSSPHGRDVERQG
                     YPIRTAEMLATLEGAAYVVRRSLHDAKNIRLAKKALRTAFEVQKQGLGFSMVELLSTC
                     PTNWGMTPADA"
     gene            complement(688..1812)
                     /gene="vorB"
                     /locus_tag="PKL60_08535"
     CDS             complement(688..1812)
                     /gene="vorB"
                     /locus_tag="PKL60_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNW96218.1"
                     /translation="MARELIKGNVAVAEAAVRAGLVSYFGYPITPQTELLEYLSARMP
                     ELGRAFVQAESELGAINMVYGAACTGRRTMTSSSSPGISLMQEGLSYIACTELPVVAV
                     NVVRGGPGLGNIAPSQGDYNQATRGGGHGEYHQLVLAPSSVQEAVDLTTLAFDLAEKY
                     RTIVMILMDGSLGQMMEPAELPPFQPLKTEVPDWAVNGVSGREKRVLTSINIDPPAQE
                     RTNIRLMTRWQEIEANEVRYKGYLLEDAEYVVIGYGTAGRIALSAVREAREKGIKVGL
                     LRPVTVAPFPVKAIDALVPGTKAFLVVEMNNGQMLEDVRLVVSGRKPIAFYGRMGGMV
                     PYPDDVLREIEALASGQISFDEHPRDRWLAQMRAEFGTGA"
     gene            complement(1802..2065)
                     /locus_tag="PKL60_08540"
     CDS             complement(1802..2065)
                     /locus_tag="PKL60_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNW96219.1"
                     /translation="MPAKGWVEINELYCKACELCITDCPSKVLALDLTRLSPKGYHPA
                     RMLHPEACTGCGICAVVCPDAAITVYRGTRPEKQAEGEVKYGA"
     gene            2320..>2534
                     /locus_tag="PKL60_08545"
     CDS             2320..>2534
                     /locus_tag="PKL60_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNW96220.1"
                     /translation="MMKKLTMAKILALMVILAFGCTPSQTQTSAPSPTFDILSNHTWL
                     MEGVVLNVLPYESKLIDFGEIKEQSDE"
BASE COUNT          504 a          759 c          716 g          555 t
ORIGIN      
        1 gcgtcggcgg gcgtcatgcc ccagttcgtc gggcaggtgg aaagcagctc gaccatcgag
       61 aagcctaagc cttgtttttg cacttcgaag gcggtgcgca gggctttctt cgccaagcgg
      121 atatttttgg catcgtgcag gctgcggcgc acgacgtacg cggcgccttc cagcgtggcc
      181 agcatctccg cggtgcggat gggataaccc tggcgctcca catcacgtcc gtgcggcgaa
      241 gaggtggtca cctgcccggg cagcgtggtt ggggccatct gaccgccggt catgccgtaa
      301 ttggtattgt tgataaaaat cgtggtgatt tgctctcccc tggcagcggc cgccacaatc
      361 tcgcccatgc cgatggaagc caggtcgccg tcaccctggt aggtgaagac catgcggtcc
      421 ggcagcgagc gtttgatgcc ggtcgccatg gccggggcgc gcccgtgcgg ggcttccaca
      481 aagtcaaaag caaagtagtt gtaagcgaac accgcgcagc ccaccggcgc cacgccgatg
      541 gtatgtccca gctcgcccat ttcttccagc acttccgcga tcaggcggtg agctacccca
      601 tgcgtgcagc cggggcaata atgcgtgccg cgctcttcaa aaccaagcgg gcgttggtaa
      661 accagtttca cgtctgatgc aatcatctca tgctcctgtt ccaaattcgg cgcgcatttg
      721 tgccagccag cggtcgcgcg ggtgttcgtc aaagctaatc tgaccggaag ccagcgcttc
      781 aatctctcgc agtacgtcat ccggataggg gaccatgccg cccattctgc cgtagaacgc
      841 gatgggcttg cggccggaga cgaccaggcg tacgtcctcg agcatctgtc cgttgttcat
      901 ttccaccacc aggaaggcct tggtgcccgg taccagcgca tcaatggctt tgaccgggaa
      961 gggcgccacc gtaaccggac gcagcagccc gaccttgatg cctttttcgc gcgcctcgcg
     1021 caccgcggag agcgcgatgc ggccggcggt tccgtagcca atcaccacgt actccgcgtc
     1081 ttccagcaaa tagcctttat agcgcacttc gttggcttca atttcctgcc agcgggtcat
     1141 caggcggatg ttggtgcgtt cctgcgccgg gggatcgatg ttaatcgagg tcagcacgcg
     1201 cttttcgcgt ccgcttaccc cgttcaccgc ccaatccggg acttccgtct tgagaggctg
     1261 gaaaggcggc agttcggcgg gttccatcat ctgccccagg ctgccgtcca tcagaatcat
     1321 gacaatggtg cggtattttt ccgccaggtc aaacgccagc gtggtcaggt ccaccgcttc
     1381 ctgcacggaa gagggcgcga gcaccaactg gtgatactcg ccgtgaccgc cgccgcgggt
     1441 agcctggttg taatctccct gggaaggcgc gatgttgccc aaccctgggc cgccgcgcac
     1501 cacattcacc gccaccaccg gcagctctgt gcaggcgatg taagacagcc cctcctgcat
     1561 caggctgatg cccgggctgg agctggaagt catggtgcgc cggccggtgc atgccgcgcc
     1621 gtataccatg ttgatggcac ccagctcgct ttccgcctgc acaaacgcgc gcccgagctc
     1681 gggcatgcgc gcggaaaggt attccaatag ttcggtctgg ggggtaatcg gatagccaaa
     1741 atacgagacc aagccggccc gcaccgcagc ttccgccacg gccacgttcc ctttaatcag
     1801 ctcacgcgcc atatttcacc tccccttccg cttgcttttc gggtctggtg ccgcgataca
     1861 cggtgatagc cgcgtccggg cagaccactg cgcagatacc gcagccggta caagcttccg
     1921 ggtgcagcat cctggctgga tgatagcctt tggggctgag tcgggtgaga tcgagtgcca
     1981 aaaccttgct ggggcagtcg gtgatacaca actcacaggc tttgcaatac agttcgttga
     2041 tttctaccca tcccttagcg ggcatgcgct tcctcctttt tatctggaaa aatttccctg
     2101 cccgatgggt gagatgagca gggttccatg tttagtatac cgtaaacccg cggcgcatgc
     2161 ggcaatcagg tgagcggcgg tagggaagat ttctacgatt ttcttcttga agaaaaagac
     2221 tattacactc ggcatgtttt cttacctctc ctggttaaaa attaataatt gacaggatta
     2281 ttatttggat aaatttgtac gaatccgcat ggagttttta tgatgaaaaa gctaacaatg
     2341 gccaaaatat tggccctgat ggtcatcctc gcatttggct gcacgccatc tcaaacacaa
     2401 acttccgcac ccagccccac tttcgatatc ctctccaacc atacctggtt aatggagggc
     2461 gtagtattaa atgtgctgcc gtacgaaagt aagcttatcg actttggtga gattaaagaa
     2521 caatctgatg aaat
//
